LOCUS       DAZPEU010000001        60420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPEU010000001 DAZPEU010000000
VERSION     DAZPEU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.001"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..322
                     /locus_tag="ACHU2L_000001"
     CDS             <1..322
                     /locus_tag="ACHU2L_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1355045.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACHU2L_000002"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACHU2L_000002"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR1355046.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACHU2L_000003"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACHU2L_000003"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR1355047.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHU2L_000004"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHU2L_000004"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR1355048.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACHU2L_000005"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACHU2L_000005"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR1355049.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACHU2L_000006"
     CDS             complement(3726..4955)
                     /locus_tag="ACHU2L_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1355050.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACHU2L_000007"
     CDS             complement(5019..5459)
                     /locus_tag="ACHU2L_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR1355051.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACHU2L_000008"
     CDS             complement(5564..6334)
                     /locus_tag="ACHU2L_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1355052.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACHU2L_000009"
     CDS             complement(6331..7257)
                     /locus_tag="ACHU2L_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1355053.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACHU2L_000010"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACHU2L_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR1355054.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHU2L_000011"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHU2L_000011"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR1355055.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACHU2L_000012"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACHU2L_000012"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR1355056.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHU2L_000013"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHU2L_000013"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR1355057.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACHU2L_000014"
     CDS             12964..13311
                     /locus_tag="ACHU2L_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR1355058.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACHU2L_000015"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACHU2L_000015"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR1355059.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACHU2L_000016"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACHU2L_000016"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR1355060.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHU2L_000017"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHU2L_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR1355061.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHU2L_000018"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHU2L_000018"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR1355062.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="ACHU2L_000019"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="ACHU2L_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHU2L_000020"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHU2L_000020"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR1355063.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHU2L_000021"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHU2L_000021"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR1355064.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHU2L_000022"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHU2L_000022"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR1355065.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHU2L_000023"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHU2L_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR1355066.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="ACHU2L_000024"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="ACHU2L_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR1355067.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHU2L_000025"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHU2L_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR1355068.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHU2L_000026"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHU2L_000026"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR1355069.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="ACHU2L_000027"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="ACHU2L_000027"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR1355070.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHU2L_000028"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHU2L_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR1355071.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /locus_tag="ACHU2L_000029"
                     /pseudo
     CDS             32769..33916
                     /locus_tag="ACHU2L_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="ACHU2L_000030"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="ACHU2L_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR1355072.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHU2L_000031"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHU2L_000031"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR1355073.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="ACHU2L_000032"
                     /pseudo
     CDS             complement(36348..36386)
                     /locus_tag="ACHU2L_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="ACHU2L_000033"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="ACHU2L_000033"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR1355074.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="ACHU2L_000034"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="ACHU2L_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR1355075.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="ACHU2L_000035"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="ACHU2L_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR1355076.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHU2L_000036"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHU2L_000036"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR1355077.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHU2L_000037"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHU2L_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR1355078.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHU2L_000038"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHU2L_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR1355079.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNPNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHU2L_000039"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHU2L_000039"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR1355080.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHU2L_000040"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHU2L_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1355081.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHU2L_000041"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHU2L_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR1355082.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHU2L_000042"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHU2L_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR1355083.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHU2L_000043"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHU2L_000043"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1355084.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHU2L_000044"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHU2L_000044"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR1355085.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHU2L_000045"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHU2L_000045"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR1355086.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHU2L_000046"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHU2L_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR1355087.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHU2L_000047"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHU2L_000047"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR1355088.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHU2L_000048"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHU2L_000048"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR1355089.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHU2L_000049"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHU2L_000049"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR1355090.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHU2L_000050"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHU2L_000050"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR1355091.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHU2L_000051"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHU2L_000051"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR1355092.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHU2L_000052"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHU2L_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR1355093.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHU2L_000053"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHU2L_000053"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR1355094.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHU2L_000054"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHU2L_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR1355095.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16732 c        14849 g        14366 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctaccca aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 caccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcgg attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgatg
//
LOCUS       DAZPEU010000002        58679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPEU010000002 DAZPEU010000000
VERSION     DAZPEU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.002"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHU2L_000055"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHU2L_000055"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR1355096.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHU2L_000056"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHU2L_000056"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR1355097.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHU2L_000057"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHU2L_000057"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR1355098.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHU2L_000058"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHU2L_000058"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR1355099.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHU2L_000059"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHU2L_000059"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR1355100.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHU2L_000060"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHU2L_000060"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1355101.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="ACHU2L_000061"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="ACHU2L_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR1355102.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="ACHU2L_000062"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="ACHU2L_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1355103.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="ACHU2L_000063"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="ACHU2L_000063"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1355104.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="ACHU2L_000064"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="ACHU2L_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR1355105.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="ACHU2L_000065"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="ACHU2L_000065"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR1355106.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="ACHU2L_000066"
     CDS             complement(15666..16703)
                     /locus_tag="ACHU2L_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1355107.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="ACHU2L_000067"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="ACHU2L_000067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1355108.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="ACHU2L_000068"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="ACHU2L_000068"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR1355109.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="ACHU2L_000069"
     CDS             19445..19885
                     /locus_tag="ACHU2L_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR1355110.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="ACHU2L_000070"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="ACHU2L_000070"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1355111.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21545)
                     /locus_tag="ACHU2L_000071"
                     /pseudo
     CDS             complement(20612..21545)
                     /locus_tag="ACHU2L_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="ACHU2L_000072"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="ACHU2L_000072"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR1355112.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="ACHU2L_000073"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="ACHU2L_000073"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR1355113.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="ACHU2L_000074"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="ACHU2L_000074"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR1355114.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="ACHU2L_000075"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="ACHU2L_000075"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR1355115.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="ACHU2L_000076"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="ACHU2L_000076"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR1355116.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="ACHU2L_000077"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="ACHU2L_000077"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR1355117.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="ACHU2L_000078"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="ACHU2L_000078"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR1355118.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="ACHU2L_000079"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="ACHU2L_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR1355119.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="ACHU2L_000080"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="ACHU2L_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR1355120.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="ACHU2L_000081"
     CDS             complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="ACHU2L_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR1355121.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="ACHU2L_000082"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="ACHU2L_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR1355122.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="ACHU2L_000083"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="ACHU2L_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR1355123.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="ACHU2L_000084"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="ACHU2L_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR1355124.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="ACHU2L_000085"
     CDS             complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="ACHU2L_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR1355125.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36827..37423
                     /gene="rsmD"
                     /locus_tag="ACHU2L_000086"
     CDS             36827..37423
                     /gene="rsmD"
                     /locus_tag="ACHU2L_000086"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR1355126.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37413..37682
                     /locus_tag="ACHU2L_000087"
     CDS             37413..37682
                     /locus_tag="ACHU2L_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR1355127.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37685..38044)
                     /locus_tag="ACHU2L_000088"
     CDS             complement(37685..38044)
                     /locus_tag="ACHU2L_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR1355128.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38185..38811
                     /locus_tag="ACHU2L_000089"
     CDS             38185..38811
                     /locus_tag="ACHU2L_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR1355129.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38885..41083
                     /gene="zntA"
                     /locus_tag="ACHU2L_000090"
     CDS             38885..41083
                     /gene="zntA"
                     /locus_tag="ACHU2L_000090"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR1355130.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="ACHU2L_000091"
     CDS             complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="ACHU2L_000091"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR1355131.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41651..42316
                     /locus_tag="ACHU2L_000092"
     CDS             41651..42316
                     /locus_tag="ACHU2L_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR1355132.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42389..42946
                     /gene="dcrB"
                     /locus_tag="ACHU2L_000093"
     CDS             42389..42946
                     /gene="dcrB"
                     /locus_tag="ACHU2L_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR1355133.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42950..44167)
                     /locus_tag="ACHU2L_000094"
     CDS             complement(42950..44167)
                     /locus_tag="ACHU2L_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1355134.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44301..45350
                     /locus_tag="ACHU2L_000095"
     CDS             44301..45350
                     /locus_tag="ACHU2L_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1355135.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45405..45992
                     /gene="acpT"
                     /locus_tag="ACHU2L_000096"
     CDS             45405..45992
                     /gene="acpT"
                     /locus_tag="ACHU2L_000096"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR1355136.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46103..47677
                     /gene="nikA"
                     /locus_tag="ACHU2L_000097"
     CDS             46103..47677
                     /gene="nikA"
                     /locus_tag="ACHU2L_000097"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1355137.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47677..48621
                     /gene="nikB"
                     /locus_tag="ACHU2L_000098"
     CDS             47677..48621
                     /gene="nikB"
                     /locus_tag="ACHU2L_000098"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR1355138.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48618..49451
                     /gene="nikC"
                     /locus_tag="ACHU2L_000099"
     CDS             48618..49451
                     /gene="nikC"
                     /locus_tag="ACHU2L_000099"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR1355139.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49451..50215
                     /gene="nikD"
                     /locus_tag="ACHU2L_000100"
     CDS             49451..50215
                     /gene="nikD"
                     /locus_tag="ACHU2L_000100"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR1355140.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50212..51018
                     /gene="nikE"
                     /locus_tag="ACHU2L_000101"
     CDS             50212..51018
                     /gene="nikE"
                     /locus_tag="ACHU2L_000101"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR1355141.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51024..51425
                     /gene="nikR"
                     /locus_tag="ACHU2L_000102"
     CDS             51024..51425
                     /gene="nikR"
                     /locus_tag="ACHU2L_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR1355142.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51545..51904)
                     /locus_tag="ACHU2L_000103"
     CDS             complement(51545..51904)
                     /locus_tag="ACHU2L_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1355143.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51901..52176)
                     /locus_tag="ACHU2L_000104"
     CDS             complement(51901..52176)
                     /locus_tag="ACHU2L_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR1355144.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52235..53359)
                     /locus_tag="ACHU2L_000105"
     CDS             complement(52235..53359)
                     /locus_tag="ACHU2L_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1355145.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="ACHU2L_000106"
     CDS             complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="ACHU2L_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR1355146.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56091..57158)
                     /locus_tag="ACHU2L_000107"
     CDS             complement(56091..57158)
                     /locus_tag="ACHU2L_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1355147.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57221..57334)
                     /gene="yhiY"
                     /locus_tag="ACHU2L_000108"
     CDS             complement(57221..57334)
                     /gene="yhiY"
                     /locus_tag="ACHU2L_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR1355148.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(57524..>58679)
                     /locus_tag="ACHU2L_000109"
     CDS             complement(57524..>58679)
                     /locus_tag="ACHU2L_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1355149.1"
                     /translation="WNWEISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTY
                     FGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNV
                     NTIISILNRRDNVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRI
                     MSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANH
                     HGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISC
                     RSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDS
                     KHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
BASE COUNT        13791 a        16263 c        14956 g        13669 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcac atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctctag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagctgcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcaacgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctacttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg ccccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaaca gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcagcac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag cgtgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg gaagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggccagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaaaatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcgg gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gcccttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgcgc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agcttgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcagattaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaacagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttctgccgc ttcgacaatc tctacggttt ccgcttcagc ctgccactct
    36301 tctgacgaaa cctcttcggc gttgacgtct tccggcaacg gcagctcttc acgttcgata
    36361 gccactggct ccggcgtttc ttctacgacc ggttccggct gtgcaacaac ttccgcttca
    36421 ggctgcgctt tttcactttc aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa
    36481 gtttccgcct cggcttcagt atgcgcctgc ggctgctctt caacggcttg ttcagaagcc
    36541 ttcgcaggct cttgcgcctg aacgatttct tctacaaccg gttgttcatt ctgaacttct
    36601 gtctcttttt ccggggtctg ctctttttga ccaaagccca gccaggaaaa aaagccacgt
    36661 cttttttctt tcgccatttg cgactacact cctcgctgtt gcttcatggc acagcgttaa
    36721 cgctatgtac atagcagcta aaaaaatgat gaaatagtct atcacttaac ttaattcaca
    36781 tcaccgcctg caagtatgtg ttatctggcg gattgagcaa tttatcatga aaaaaccgaa
    36841 tcattccggc agcggccaaa tccgcattat tggcgggcag tggcgaggcc gtaaactccc
    36901 ggttcctgat agcccaggtc tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa
    36961 ctggctggct ccggtcattg ttgacgccca atgtctggat tgcttcgccg ggagcggcgc
    37021 gctggggctg gaagcgttat cgcgctacgc tgcgggggca acgttgattg agatggatcg
    37081 cgtggtttct cagcagttaa ttaagaatct ggcgacacta aaagcaggca atgcacgcgt
    37141 ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc acaccgcata atatcgtgtt
    37201 tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg ataaatttac tggaagataa
    37261 cggctggctg gctgacgaag ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc
    37321 cactgttccg gcaaactggt cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg
    37381 gctgtatcaa cacgaagcac aaggagaaag tgatgctgat taatattggt cgtttgttaa
    37441 tgctctgcgt ttggggattt ttaatcctca atctggtgca tcccttcccg cgcccgctaa
    37501 atattttcgt taacgtggcg ctgattttta ccgtgctgat gcatggtatg cagctggcgc
    37561 tattgaaatc cactttaccg aaagatggcc cgcagatgac caccgccgaa aaggtgcgga
    37621 ttttcctttt cggcgtgttt gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat
    37681 aaccttattg ttcgccaaca aagctaacaa agcgcgttcc tttatagctc agcgtacctt
    37741 tatcgcccac tgtcagggcg tggtactgct gggcgtcgag gcgaaacgtc tgctccattc
    37801 ctccgctttg cggtttgaag cttgcctcat agcgcatact ggtgcctgcc ggagtcactt
    37861 cctgctggcg cgaacggcga tcgttaatcg gtttttcccg cttgttgctc accaccacca
    37921 gcttttgctg gagcggagcc atatcattat cagctttttc ccgtcgctgt tgcataaaac
    37981 gaaacgatgc ggcgacgaca attaagccaa tgataacaat aaagaaaaga ggtggtttac
    38041 tcatctttat ccctcatcgg aaaatgcgga attaagcata ccctgccagt tatggtgttg
    38101 tcatccgtcc accctcgcca ctaaactgga agcaagaccg taggcattct gcttacgaaa
    38161 aaataacgaa ttcaaggaac taagatgctt tggtcgttta tcgctgtctg tctttccgca
    38221 tggctatctg tggatgcatc gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg
    38281 ttaacccttc ttctcctgct gttactggcc tggcaagcac cgatgttcga cgccattagc
    38341 tatctggtgc tggcgggact gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca
    38401 cgtcaacgtc tgatgtacgc catcggcgcg tttttcctct cgcacctgct gtacaccatc
    38461 tatttcgcca gtcagatgac gctctctttc ttctggcctc tgccactggt gttgctggtt
    38521 ctgggtgcgc tgctactggc gattatctgg acgcggctgg aagagtaccg ttggcctatc
    38581 tgcacgttta tcggcatgac gctggtgatg gtgtggctgg caggtgaact gtggttcttc
    38641 cgtccgaccg ctccggcgct ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac
    38701 tttgtctggc tggggagcca ctatcgccga cgcttccgtg cggataacgc gattgctgcg
    38761 gcctgctact ttgccggtca cttcctcatc gtccgctcac tgtatctctg ataaaacttg
    38821 actctggagt cgactccaga gtgtatcctt cggttaatga gaaaaaactt aaccggagga
    38881 tgccatgtcg acacctgaca atcacggcaa gaaagcccct caatttgctg cgttcaaacc
    38941 gctaaccacg gtacagaacg ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc
    39001 aacgctctct gaaaacgtct ccggcacccg ctatagctgg aaagtcagcg gcatggactg
    39061 cgccgcctgt gcgcgcaagg tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt
    39121 gcaggtgttg ttcgccaccg aaaaactggt ggttgatgcc gacaatgaca tccgtgccca
    39181 ggttgaatct gcggtgcaaa aagcgggcta ttccctgcgc gatgagcagg ccgccgacga
    39241 gccacaagca tcgcgcctga aagagaatct gccgctgatt acgcttatcg tgatgatggc
    39301 aatcagctgg ggtctggagc agttcaatca tccgttcggg caactggcgt ttatcgcgac
    39361 cacgctagtt gggctgtacc cgattgctcg tcaggcatta cggctgatca aatccggcag
    39421 ctacttcgcc attgaaacct taatgagcgt agccgctatt ggtgcactgt ttattggcgc
    39481 aacggctgaa gctgcgatgg tgttgctgct gtttttgatt ggtgaacgac tggaaggctg
    39541 ggccgccagc cgcgcgcgtc agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc
    39601 cacgcgcctg cgtaacggtg agcgggaaga ggtggcgatt aacagcctgc ggcctggcga
    39661 tgtgattgaa gtcgccgcag gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt
    39721 tgccagtttt gatgaaagcg ccctaaccgg agaatccatt ccggtggagc gcgcgacggg
    39781 cgataaagtt cctgcaggag ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc
    39841 agaaccgggt gccagcgcca ttgaccggat tctgaaactg atcgaagaag ccgaagagcg
    39901 ccgcgccccc attgagcggt ttatcgaccg tttcagccgt atttatacgc cagcgattat
    39961 ggccgtcgcc ctgctggtaa cgctagtgcc gccgctgctg tttgccgcca gctggcagga
    40021 gtggatttat aaaggtctga cgctgctgct gattggttgc ccgtgtgcgt tagttatctc
    40081 aacgcctgcg gcgataacct ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa
    40141 aggcggtgcg gcgctggaac aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg
    40201 tacgctgacc gtcggtaaac cgcgcgttac cgcgattcat ccggcaacgg gtattagtga
    40261 atctgaactg ctgacactgg cggcggcggt cgagcaaggc gcgacgcatc cactggcgca
    40321 agccatcgta cgcgaagcac aggttgctga actcgccatt cccaccgccg aatcacagcg
    40381 ggcactggtc gggtctggca ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc
    40441 tgccgggaaa catcccgctg atgcatttgc tggtttgatt aatgaactgg aaagcgccgg
    40501 gcaaacggtt gtgctggtag tacgtaatga tgacgtgctg ggtatcattg cattgcagga
    40561 taccctgcgc gccgacgcta caactgccat cagtgaactg aacgcgctgg gggttaaagg
    40621 agtgatcctc accggcgata atccacgcgc agcggcggca attgccgggg agctggggct
    40681 ggagtttaaa gcgggcctat tgccggaaga taaagtcaaa gcggtgacca agctgaatca
    40741 acatgcgccc ctggcgatgg tcggtgatgg tattaacgac gcgccagcga tgaaagctgc
    40801 cgccatcggg attgctatgg gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc
    40861 attaacccat aaccacctgc gcggcctggt gcaaatgatt gaactggcac gcgccactca
    40921 cgccaatatc cgccagaaca tcaccattgc gctgggactg aaagggatct tcctcgtcac
    40981 cacgctttta gggatgaccg ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt
    41041 gctggtgacg gcgaatgcgt taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac
    41101 attgagcgcg atcggtcccc tcgcccctct ggggagaggg ttagggggtg aggaaaaggc
    41161 ggcatcgaag ccaatcagcc cctatcaacc gcctttacga atcaaataac gataaggcag
    41221 tccatccgtc tctttcgcaa ccagttcgtg ttccataaag gtacaaaacc caggaatatc
    41281 gcgggtagtg gccggatcgt cggcgataat cagcaacgtt tcgccaggct gcatattgcg
    41341 cacggttttg cgcaccatca tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt
    41401 gtggtcaggg ctggaaaaga gatcggtcat tttcttctca tcacttaaaa aaacggcgct
    41461 agtttacgcc ctgtgagtcc gtaagcaaac caggttaacg attgcgtgaa aattaaccat
    41521 tgcattgtca acgtaaagca gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt
    41581 atgttaaggt aacagacgtg tcgtacgtat tgggttccct caccccaatg gttaatcaaa
    41641 aaggtacaat atgaacgttt tctcgcaaac tcaacgctat aaggcgttgt tctggttatc
    41701 gttatttcat cttctggtga tcacctccag taactatctg gttcagcttc ccgtctccat
    41761 ttttggtttc cataccacct ggggcgcgtt tagctttccg tttatttttc ttgctaccga
    41821 cctgaccgtg cgtatttttg gcgcaccgct ggcccgacgc attatcttcg cggtaatgat
    41881 ccctgcgtta ttaatctcct acgtcatctc gtcgctattc tatatgggtt cctggcaggg
    41941 attcggcgca ctcgcccact tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat
    42001 ggcctacgcg ctggggcaaa tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg
    42061 ccgctggtgg ctggcaccga cagcgtccac actgttcggt aacgtcagcg acacgctggc
    42121 ctttttcttc attgccttct ggcgtagccc ggatgccttt atggctgaac actggatgga
    42181 aatcgcgctg gtcgattact gtttcaaagt gttaatcagt atcgttttct tcctgccaat
    42241 gtatggcgta ttactcaata tgctgttgaa aagactggca gataaatccg aaatcaacgc
    42301 tttgcaggcg agttaaaggt tcgttatcag agttgtgata agatggatga atgagccgtt
    42361 atggccgttt atcgaaagga agaagtcaat gcgcaatctg gttaaatatg tcggaattgg
    42421 cctgctggtt atggggcttg cggcctgtga tgataaagac actaacgcta cggcgcaggg
    42481 ttcggttgcg gaaagtaacg ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag
    42541 tttctcgctg ccagcggata tgaccgacca gagcggtaag ctgggaacgc aggccaataa
    42601 catgcatgtc tggtccgacg ccaccgggca gaaagcagtc atcgtcatca tgggcgatga
    42661 tccgaaagaa gatctggcgg tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga
    42721 tccgcagctg caagtggtaa ccaataaagc cattgagctg aaaggtcaca aaatgcagca
    42781 gttagacagt attatctccg cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa
    42841 cgtgggtaat caactgctga ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc
    42901 gcaaaccacc gcagaaaaca tcattaatac gctggttatt cagtaagttt taagatgatg
    42961 aggcggcctc agggacgtgt tccggaggcc gtttttttaa tcgccacgtc agtaataacg
    43021 cgattgcgac cagtcccgcc gccgccagat aaatcaccgg tacgcccgcc cagctcatca
    43081 ccagcccagc cagtggtcca gtcacgccaa gcgataaatc cataaatacg gtgtaagttg
    43141 ccagcgccgc cccctgattt tgctgcggaa ccgcttttac cgccactaca cccaatgccg
    43201 ggaacaccag cgaaaacccg gcccccgcca gtaagacgcc gattttcgcc atccacggca
    43261 tagtcgccac gccaaccagt agcaggccga ttatctcaac gctaaagcaa atcatcgcta
    43321 cgtttaagcc accgatacgg ttaatgccgt tagggaataa caaacgcgta ccgacaaacg
    43381 cacagctaaa cagcgtcagc gcgaaagccg caccgtccca acctttagcg tcataaaaca
    43441 gcgtgataaa ggtggcgatg acgccaaatc cggcggaagc cagtgccagc gccataccgt
    43501 acagccagac gcgcccaagc accgcgcgaa acggcagcgg tttgccttta ctggctttta
    43561 ccgtcggacg cgggatcgcc aacaaaatgg ccaccagcgc cacgcccata atgattaacg
    43621 ctaacgcctg caagccgccc cagtgataaa acacgacgcc taacggcgca cccatcgcca
    43681 tcgccccgta agtgacaatg ccgttccacg aaatcacccg cccgatatgc agcgagccaa
    43741 ccactccaac gccccatagg gtcgatcccg ttccggcaaa actttgccca atcccaagga
    43801 tgacgcgccc caggcaaagt aataacaggc tgatgacagg cagactggcg gttaatcctg
    43861 ccgtcagata ccccagaccg ctcaaaaagc agccgcataa accgaagacg acaatctttt
    43921 tgggtcccag caaatcggcg taacgtccgg catgagggcg gctcagcaag gtggcgaaat
    43981 attgcaggct gataaccaat cctgcccaga aggcgctaaa gcccatcaca tcatggacat
    44041 agcccggtaa tacagcgagc ggcaacccga tggtgaggta gctggcgaag ttaaacatga
    44101 caatggagac aatgcgcaaa ttcaggcgca atccgtttag cgcgggttcg gctacgggtt
    44161 cgggcatgag gatcaccaca tttttacaac agtgtttcat ttttaccatg tgctgacgtg
    44221 aaaatcagca gtaagaatca gaatattgct ggcgtgactc ccgcgctaca cttaacacaa
    44281 aaagtcacaa ggaagcccca atggaaaccc ctcaacccga taaaacgggc atgcacattc
    44341 tgctcaagtt ggcctcgctg gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca
    44401 ttgtgcagct gttactggcg ctgttttttg ccatcgtcct caacccgctc gttacctggt
    44461 ttattcgtcg gggagtacaa cgccccgttg ccattacgat tgtagtggtg gtgatgctga
    44521 tcgcactaac cgcgctggtc ggcgtactgg cggcatcgtt taacgaattt atctctatgc
    44581 tgccgaagtt taataaggag ctgacgcgca aactttttaa attgcaggag atgttgcctt
    44641 ttcttaattt gcatatgtcg ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg
    44701 ttaccttcac cacagcgcta atgaccgggc tttccggggc aatggcgagc gtgcttttgc
    44761 tggtgatgac cgtagttttt atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt
    44821 ttgcgctgaa taacccacag attcacatcg caggattaca ccgcgcactt aaaggcgttt
    44881 cgcattatct ggcgttaaag acgctactca gtttatggac aggtgtcatc gtctggctgg
    44941 ggctggcgct aatgggcgta cagtttgcgc tgatgtgggc agtactggcg tttttgctca
    45001 actacgtgcc caatatcggc gcggtaattt ccgccgtacc gccaatgatt caggtgctgc
    45061 tgtttaatgg tgtttacgaa tgtattctgg tcggcgcatt gtttttagtg gtccatatgg
    45121 tcatcggcaa tattttagaa ccacggatga tgggccatcg cctggggatg tccaccatgg
    45181 tggtatttct ttcattgtta atctggggat ggctgctcgg cccggtaggg atgctacttt
    45241 cggtgccatt aaccagcgtg tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac
    45301 tggcgatttt actggggccg ggcagaccga aaagtcggtt accgggatga ggcgacaagt
    45361 gatacgatac gcactttcat tttccattaa acgttggccc tgatatgtat cggatagttc
    45421 tggggaaagt ttcgacctta agcgcagctc cactgccacc gggtttacgc gagcaagcac
    45481 cgcaaggtcc acgacgcgaa cgctggctgg cggggcgtac attgctttcg cacacgcttt
    45541 ccccgctacc ggagatcatc tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc
    45601 cgctatggtt caacttaagc catagcggtg acgatatcgc tctgctgttg agcgatgaag
    45661 gcgaagtcgg ctgcgatatc gaagtgattc gcccgcgcgt caactggcgc tggctggcga
    45721 acgccgtgtt cagcctcggg gaacacgctg aaatggacgc cgtgcatcct gatcagcaac
    45781 tggaaatgtt ctggcgcatc tggacgcgca aagaagccat cgttaaacag cgtggcggca
    45841 gcgcctggca aatcgtcagc gtagatagca cctatcactc ctcgctgtca gtcagccatt
    45901 gtcagctaga aaatttaagc cttgcgatct gcacccccac cccctttacg ctcaccgccg
    45961 acagtgtgca atggatcgat tcagttaact gatccgccca cccgactgcc catctattga
    46021 tccagaacag gtaatcagta tgacgaatac ttaaaatcgt catacttatt tccgccatct
    46081 attttaatcc attggggtta ccatgctctc cacactccgc cgcactctat ttgcgctgct
    46141 ggcttgtgcg tcttttatcg tccatgccgc tgcaccagat gaaatcacca ccgcctggcc
    46201 ggtgaatgtc gggccactaa acccgcacct ttacacgcct aaccagatgt tcgcccagag
    46261 catggtttat gaaccattgg tgaaatatca ggcagacggt tcggtgatcc cgtggctggc
    46321 aaaaagctgg actcattcag aagatggtaa aacctggacc ttcaccctgc gtgatgacgt
    46381 gaaattctcc aacggtgaac cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt
    46441 gctcgataac cgtcaacgtc acgcctggct ggagctggca aaccagattg ttgatgttaa
    46501 agcactcaat aaaacagagc tgcaaattac cctgaaaagc gcctactatc ctttcctgca
    46561 agaactggcc ctgccccgtc ctttccgttt tatcgctccc tcgcagttta aaaaccatga
    46621 aaccatgaac ggaattaaag cgccgattgg caccggaccg tggattttgc aggaatcgaa
    46681 actaaatcag tacgatgtct tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat
    46741 taaaaagatc acctttaacg tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac
    46801 tggcgatatc gacctgctgt acggaaacga agggttatta ccgctcgata ccttcgcccg
    46861 ctttagccag aatccggctt accacaccca actgtcacag ccgatcgaaa ccgtgatgct
    46921 ggcgctcaat accgccaaag cccccaccaa cgagctggca gtacgtgaag ctcttaatta
    46981 cgcggtaaac aaaaaatcgc tgattgataa cgcgttatat ggcacccagc aggtcgccga
    47041 caccctgttt gccccttctg tgccttacgc taatctcggc ctgaaaccgc gccagtacga
    47101 tccgcagaaa gcgaaagagt tgctggaaaa agccggttgg acgctgcctg cgggcaaaga
    47161 catccgcgag aaaaatggtc agccgctgcg cattgaactg tcgttcatcg gcaccgatgc
    47221 gttaagcaaa tcgatggcgg aaatcattca ggctgatatg cgccagattg gcgcagatgt
    47281 ctcgctgatt ggcgaagaag agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg
    47341 catgattttc caccgcacct ggggcgcgcc atacgatcca cacgccttcc tgagttcaat
    47401 gcgcgtaccg tcgcacgctg acttccaggc acagcaagga ttagccgaca agccgctgat
    47461 tgataaagag atcggcgaag tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta
    47521 tcgcgacatt ctgacccgtc tgcatgacga ggcggtttat ctgcctatca gctacatctc
    47581 gatgatggtg gtatcaaaac cggagctggg taatatcccc tacgcgccga tcgccaccga
    47641 aattccgttc gaacagatta aaccggtgaa accttaatgt tgcgttacgt attacgccgc
    47701 tttctgctgc tgatcccgat ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc
    47761 ctcggtaccg gcgacccggc gctcgattat ctgcgtttat ctaacctgcc gccgacgccg
    47821 gagatgctgg cctctacccg caccatgctc gggctggatc agccgctgta cgtccagtac
    47881 ggtacctggt tgtggaaggc gctgcatctt gactttggta tctcattcgc cagccaacgc
    47941 ccggtactgg acgatatgct gaacttcctg cccgccacgc tggaacttgc aggtgcggcg
    48001 ctggtattaa ttctgctcac ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat
    48061 cgtttgccgg atttcgccgt gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc
    48121 tggctggcat ttttgctagt gatggcgttt tcggtgtatc tgcaatggct acccgcgatg
    48181 ggctacggca gctggcagca catcatttta cctgcggttt ccatcgcctt tatgtcgctg
    48241 gcgattaacg cgcgactgct gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc
    48301 acctgggcgc ggctacgcgg cctgaatgac aaacagaccg aacgccgcca tattctgcgc
    48361 aatgcctcgc tgccgatgat caccgccgtg gggatgcaca ttggcgaatt gattggcggg
    48421 acgatgatta tcgaaaacat ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg
    48481 atttttaacc gtgactatcc ggtgattcag tgctttacgc tgatgatggt ggtggttttt
    48541 gtggtctgta atttgattgt cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt
    48601 catgaaggag cgcacgcgtg aactttttcc tctcttcccg ctggtcggta cgcctggcgc
    48661 tgatcattat cgccctgctg gcgctgattg cgctcaccag ccagtggtgg cagccgtacg
    48721 atccacaggc aattgatttg ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg
    48781 gcaccgatca cttaggtcgc gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc
    48841 tcggttcggt aatggcctgc ctgctgctgg tgctgacatt agggctggtt attggcggca
    48901 gcgccgggtt gattggcggg cacgttgatc aggccaccat gcgcgtcgcc gatatgttta
    48961 tgaccttccc gacctcgatt ctgtcgttct ttatggttgg cgtgctcggc accgggctga
    49021 ccaacgtaat tatcgccatc gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca
    49081 gcttggtgat ttcactacgc caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg
    49141 gccatgtgcg ggtgtttgtc gatcatctgg caggcgcggt gatcccttcg ctgctggtgc
    49201 tggcaacgct ggatatcggc catatgatgc tgcacgtcgc ggggatgtct ttccttggcc
    49261 tcggtgtgac cgcgccgacc gccgaatggg gcgtgatgat taacgacgcg cgccagtata
    49321 tctggaccca gccgctgcaa atgttctggc cggggctggc gctgtttatc agcgtgatgg
    49381 cctttaacct ggtgggtgac gcactgcgcg atcatctgga tcctcatctg gtgacggagc
    49441 acgcacacta atgccgcaac agattgaact acgtaatatc acgctacagg ccgcgcagcc
    49501 gctggtacac ggtgtatcgt taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg
    49561 tagcggcagc gggaaatcat taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt
    49621 tcgccagacg gcgggggaaa ttttagccga tggcaaaccg gtttcgcctt acgccctgcg
    49681 cggcatcaaa attgccacca tcatgcagaa cccgcgcagc gcctttaatc cactgcacac
    49741 catgcacacc cacgcgcggg aaacctgcct ggcgttaggg aaacccgccg atgacgctac
    49801 gcttaccgct gccatagaag cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta
    49861 cccgttcgag atgagcggcg gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg
    49921 tgaatcaccg tttatcatcg ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc
    49981 gcgcatcctc gatctgctgg aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt
    50041 cacccatgat atgggcgttg tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg
    50101 taaaattgtc gaacagggcg atgtagaaac gctgtttaac gcccccaaac atacggtgac
    50161 gcgcagcctg gtttccgctc atctcgccct ctacggtatg gagctggcat catgacctta
    50221 cttaacgtct gcggcctttc ccatcactat gcccacggtg gatttagcgg aaaacatcaa
    50281 catcaggcgg tgctgaataa cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg
    50341 ctgggacgca gcggctgcgg aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct
    50401 ccctcacaag ggaatattag ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa
    50461 cgtaaagcct tccgccgtga tattcagatg gtgtttcagg actccatcag tgccgtgaat
    50521 ccgcgcaaaa ccgtgcgcga gatcctgcgt gaaccgatgc gccacctgct atcactgaaa
    50581 aaatccgaac aactggcgcg cgccagcgaa atgctgaagg cggtcgatct cgatgacagc
    50641 gttctcgaca aacgcccacc gcagttaagc ggcggccagc tccagctcgt ctgcctggca
    50701 cgcgcgctgg cggtcgaacc gaaattgctg attctggatg aagccgtttc taaccttgat
    50761 ctcgtgttac aggcgggcgt cattcgcctg ctgaaaaagc tacaacaaca gtttggcact
    50821 gcctgcctgt tcatcactca tgacttacgc ctggtggaac gcttttgcca gcgagtgatg
    50881 gtgatggaca acggacaaat cgccgaaacc caggcggtgg gagataaatt aaccttttcc
    50941 tctgacgccg gacgtgtgct acaaaacgcg gtattacccg cattccccgt gcgccgtcgc
    51001 acctcagaaa aggtttaacg ccaatgcaac gagtcaccat cacgcttgat gacgatttac
    51061 tggagacgct ggacagcctg agccagcgtc gtggttataa caaccgttcc gaagctatcc
    51121 gcgatattct gcgtagtgcc ctggcgcagg aagctaccca gcagcacggc acacaaggtt
    51181 tcgcggtgct gtcgtatgtg tatgaacacg aaaaacgcga cttagccagc cgtattgttt
    51241 ccacccagca tcatcaccac gacctctccg tcgccacgct acatgtgcac atcaaccacg
    51301 acgactgtct ggaaatcgcc gtgttgaaag gtgacatggg tgacgtgcag cattttgccg
    51361 atgacgttat cgcccagcgc ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag
    51421 attgagttat ttctaaaaca aggaaagaaa agatattaac tgaacttaat ttataaaatt
    51481 aagttcagtt gcatagatag atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta
    51541 aaatttataa atgattcaaa cgctcattca gtgtttcaat aatataggta ttaacagaga
    51601 cttgttgctg cgcagccgca ttattaaggc gttcactcag cgattctgga tagcgtaacg
    51661 taaaggtctt aatcttttct gtacgcgcat acggctcaat acccgcagct ttacaatcct
    51721 caagatactc tcgcagggaa agctctcctt ctttttgcag cccctgaata ctgtcagaga
    51781 caaaatcaca ataacctgac aagcccagga atttgccacg aaatgcattc agttctggca
    51841 cgtaggtaat aacagctggt tgcccggcaa tctccataga gtttggcgtc ttcagtttga
    51901 tcatggtttt acccctatac ttagtaacca gtcacacacg ctttctacag cgcctttatc
    51961 ggtatctggc gacggatgag gccgatggaa acacgcaacg ctcatattta gtatgaactt
    52021 gcaacgcgaa cctcttcctt ccttaatttc tccgcctaat gctttaacca gtgactctat
    52081 atcagaccac ttgatccctt gaggaacagg agttttaaat atttgatcca atgtattttt
    52141 ttgtttcttc cgtaaagcct gtaccttggt tctcatggca tgtccttatg acttcacttt
    52201 atgacatcac agtatgaagt caagtttccc ttccttacgc cattgtccca atcgtcttcc
    52261 tgaatcgcag cagtgcgatg gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact
    52321 gcggccagac gatttcgaat ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa
    52381 agtgtgtcgt cggcatggtc agcataatgt cctgcaccat ctgcggcata ctttcgcgcg
    52441 gcgtggatcc accggaaagc atttgcagcg gcagcagcac cagaatcacc agcagcccca
    52501 gttgcggcat tgaacgcgct atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca
    52561 gactgagcgc cacgcccagc ataaacagcg ggatcgagcc ttcaatcggt acgcccagta
    52621 cacctttcac catcagcacc agcgataatc ccgataccac cagcaccacc agccccatcg
    52681 accagatctt cgccatcatg atctcaaacg gcgttatcgg catcaccagt aaatgttcca
    52741 ccgtgccgtg ttcacgctcg cggatcagcg ccgatccggt caatacaatc gccagcatgg
    52801 taatgttgtt gatgatcgcc atcaccccgc caaaccacgc gggatcaagg ttcgggttaa
    52861 agcgcatccg ggtttccagc gataccaacg gttcgctgtt atcacggtag cgcgcgacaa
    52921 agctgttcac ttcaccgttg ataatattct ggatataccc attgccggta aatgcctggc
    52981 tcatgcgcgt ggcatcgacg ttcacctgaa tatccggctg gcgtccggcg aggacatcac
    53041 gctgaaaatt aggcggaata tttatcgcga aggtatagcg tccggcgtcc agtccggcat
    53101 ccatctcatc ggcggtgatc atctccggtg gcaaaaacca cggacgatag aagctgttaa
    53161 cgatccggtt cgataactgc gattgatcca tatcggcaat ggcgatcggc gcgaggttca
    53221 acgatcctgg cgtaacggtc gctgacgaat acaccgacac cgtaaacgag aagacaatca
    53281 gcgtcagcat cgctttatca ccgagcagac tgcgcaactc tttgataccc agattaaaaa
    53341 tattgcgtaa atggcgcatc atccctcctg ttttttcagc agcaggatgc ttaagcccat
    53401 caccagcggg atggctatca gcaacgggat aaaaagttgc cacaaatcag tcagatccag
    53461 cgctttcgag aacgttccgc gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc
    53521 gatccaacgt ccaggccctt ccagcgaagc taccggatcg atcatcccgg aaaactgtgt
    53581 cgccgggatc aatgtgataa tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa
    53641 ggtggagatc agcagcccca ttccagtggc aatgatgata tacagcagcg ccgccaaggt
    53701 gagcgtcagg aaactgcctt tatgcggtac gccaaacaca aacaccgaca ggccgcagag
    53761 caggaaaaag ttcagcatcc ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc
    53821 gctacgggtg gtgggggtca cgtaaaggtt gataatcgac cctagctctt tttcccgcac
    53881 cacgctaagg gcgcttagca ttgacgggat catcatcagc agaagcggga tcaccgccgg
    53941 aacaatcgct ggcaggcttt ttacgtccgg gttatagcga taacgcgtct caatgttcat
    54001 cagcccgctt tggctggcgg gtgtcgattg tcggctcgcc acatcctgaa gccaactctg
    54061 gtgcattgcc tgcacgtaac cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat
    54121 ccagacgccg agttccacag gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat
    54181 ctcaatggcc accgtgatat cgcccgcacg catccgacga tcaagctcgt cataactggt
    54241 gagcggcggc tgttcgataa agtaacggga accggagagg ttgagtgtcc acgcctggct
    54301 actgacggtc tggtcgcggt cgagcaccgc aaagcgcagg ttttccacat ccatactgat
    54361 gccgtaaccc attatcagca tcaggatcac cgttcccatc agcgccagcg tcgaacgtac
    54421 tggatcgcgt cgcagttcca gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct
    54481 aaatccctga cgcggcgcgt gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt
    54541 gctctgccct gccgcttcct gcaaataggc gataaatgcc tcttccagac tggcggctcc
    54601 gcgtttctca accagttcct gcggtgtacc gctggcaagc acttttccgg cgtgcatcag
    54661 tgagatgcgg tcgcaacgtt ccgcttcgtt cataaagtgg gtggagatga agatagtcac
    54721 tttgtcctgg cgcgagagat cgaccatcaa ctgccagaac atatccctcg ccaccggatc
    54781 gacaccagaa gtaggctcat cgaggattaa catctccggg cgatgaatca ccgccaccgc
    54841 cagcgaaagc cgctggcgaa tgccgagcgg caatgactcc ggcagaacat cttcaacgtc
    54901 gttgagctta aaacgctcgc tcatttcagc cactcgcgcg ggaatttccg cttccgggat
    54961 gtgaaacaaa cgggcatgta actccaggtt ttgccgcacg gtgagttcgt tatagagcga
    55021 aaacgcctgc gacatatagc ccacacgacg gcgggtatcg atatcttttg gatcaaccgg
    55081 ttgcccaaat agccacgcct caccttcgct ggcgggcagc agtccggtaa gcattttcat
    55141 ggtggtggat ttaccgcagc cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat
    55201 gcggaaattg acgtgatcaa cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc
    55261 ttcgatggca atctctgcgt tttcaggttg atacggtggg atcactaccg cctgatgcgc
    55321 ctggcgttgc gcttgcggta acagattaat aaatgcttct tccagcgtgg cgctttgcgt
    55381 ttgctgccgc agctcttcgg cgctgccggt tgccagcact tctccggcat tcatcgctac
    55441 cagccagtcg aagcgttcgg cctcttccat ataggcggtg gcgaccagca cgctcatatt
    55501 gctctgccgc tggcgaatac tgtcgatcag atcccagaac tgggcgcggg agagcgggtc
    55561 aacccccgtt gttggctcat caaggatcaa cagttccggg tcgtggatta acgcgcagca
    55621 cagcccaagt ttttgcttca tcccgccgga gagtttacct gccggacgat cgcgaaacgg
    55681 tgctaacccg gtgctggtca gcagctcatt aattcgcgct tcccgctccg ctttatcgtg
    55741 accgaacagg cgagcgaaaa aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt
    55801 tttgcccagc ccctgcggca tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg
    55861 gtcgtgcata tcgccaccca gcaccaccac attgccctgc tcaatgacgc gggcaccgga
    55921 aatcagtgac aataaactcg acttcccgac gccatccgga ccaatcagcc cgaccatgca
    55981 acgggcctga atatcgagag tgatattgtt cagcgcaacg gtttttccat aatgctggct
    56041 cacgcccgcc agttgcgcga caggcgggac gggaaccagt tccagatgcg tcattgcggc
    56101 aacctcacca cgaggtcgtc aggccacgga agttcttcat tcacccgcac ccacgctacg
    56161 cccggtaaac cggttttgac atattccaga tgctgctgga gtaattccgg tgggatacgc
    56221 gctttgacgc ggaacatcag tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg
    56281 aactgggcga cactggcgac aaaactgatg gttgcaggaa tacgcagatc tggcgcggca
    56341 tcgaggatca gccgggcttc accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg
    56401 aagaaggtca tatagacgtc gctgagatcg accatattca gcacccgacc gcctgccgcc
    56461 agcacttccc ctggctcggc aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt
    56521 tcactgtcat cgatatctgc ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga
    56581 gtttgcgcct gaatgatatt ggtgcgtgct gcttctatag ccgctttaga agccgaaacc
    56641 tgggctttcg ctgattccag cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc
    56701 agctgttgcg cagaaatagc ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg
    56761 gcaacagagt ccagttcggc ctggcgttga ttgaccagcg actgtgcggc acgagtttca
    56821 ctttgtcgtt gctccagcaa agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt
    56881 tgcgcgatgg cttccagtcg ctgttcctgc aacacgcgag tatccatctt cgccagcact
    56941 tcgccttcgc gaacaaactg gccttctttc accagaatgg tgtcgatacg cccggcaatt
    57001 ttgctggcga tatccacttc cgtcgcttca atgcgcccat tgctgacagc aaagccttcc
    57061 ggcacacccg ccgggcgcaa cagccaccac gccacgacgg cagccaccgc cagtgccccg
    57121 acaacccacc acgccagatg gcgcttactc ttatccataa tcgacccgcc ataatccctg
    57181 taagcaaacg acgcgtgtta caactgccgc cgaacgacgt tcagcggatt cttccatccg
    57241 ggataagaag gaaacgacaa atacgaccag cgcgcagagc attgagcgcc aacggggaag
    57301 gcttagtgaa aacgggtagt aatgttgtca tcatcagcat tcctggccgt aaatgaaaca
    57361 cgcatactca taagtgtgat ctataaaact ggctgacaaa acggtatcca tcctgttgga
    57421 tcgcatttca cgcctgttca gttgcaggaa cagagcattc ctttttacgt tttatcaaca
    57481 gtctcaacca gatagtaaca ttaatattca tgttgtctat ggttcagagc tgtaacattg
    57541 tgcgccttct aatcaaaggc ataagtccat ttctgttttt ttcatgctgg taagacttcc
    57601 agggagtggt tatctctggc cctgcaccag ggttaatttg gcaattatgc atgctgaaca
    57661 ttcttccagc atgtttactg tcacgggcgt taagacctaa aactgtcccc gtttcaccta
    57721 tgcgttctct cccaccattt ccgttatgtg caacaacttg atttatacct aacatttgtg
    57781 aacatgctaa tagtgcttca agtttaggtc tgtaatctgt cgaaacagaa aaataacgat
    57841 taaaggatcg acaagagata gcccggctag ttttaaactt tttgccatta attatagcat
    57901 gatgcttttt attaataatt tccgctaatt cgaccggatc gagagcattg gtcatttcat
    57961 caatagagac agttatcggt cctataatgt aacgttttcg ctgatcatca aaagttatcc
    58021 catgatgatt tgccattatt ttgtatttgg ggtcatagaa acatacagga taatctttta
    58081 tcccgtcgta ggtatcctca tcaagtggtt tcatgcgcgt gaagttttgc atatagttac
    58141 cattgcaatt cgtttcatga tttccagcta atacatgaac attttcattg atactcatta
    58201 tgcgttttaa caaatcgatg ataaattgat cgccacagat attactaaat cgatcgccgg
    58261 tttggtcgcc aagaaacgta catggcgtga ctgcgtcttt attaataacc aaataaggct
    58321 gaagactttc taaaacatta tctcttctgt tcagtatact gattattgta ttaacattag
    58381 tgtcaagttg ctcattaaag ttctccctta gcaatacgtt gagaacttca tcttctatat
    58441 taagcaactg gctaaggaaa ttttctcctt cagccatgat tcccaaatgt cctgttgcaa
    58501 aaagcgcgta caaaactgca ccgactgagc catcggtatc gccaaaatag gtcggcctgg
    58561 tacttgtggg atcaacaggt gaacaattgg agaaataggt tctgtcagcg aactggggtt
    58621 ttataattgt ttcgaattta atatcaaata cattgtattg cgatatttcc cagttccag
//
LOCUS       DAZPEU010000003        58547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPEU010000003 DAZPEU010000000
VERSION     DAZPEU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.003"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            120..2654
                     /gene="mrcB"
                     /locus_tag="ACHU2L_000110"
     CDS             120..2654
                     /gene="mrcB"
                     /locus_tag="ACHU2L_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR1355150.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2874..5117
                     /gene="fhuA"
                     /locus_tag="ACHU2L_000111"
     CDS             2874..5117
                     /gene="fhuA"
                     /locus_tag="ACHU2L_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR1355151.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5168..5965
                     /gene="fhuC"
                     /locus_tag="ACHU2L_000112"
     CDS             5168..5965
                     /gene="fhuC"
                     /locus_tag="ACHU2L_000112"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR1355152.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5965..6855
                     /gene="fhuD"
                     /locus_tag="ACHU2L_000113"
     CDS             5965..6855
                     /gene="fhuD"
                     /locus_tag="ACHU2L_000113"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR1355153.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6852..8834
                     /gene="fhuB"
                     /locus_tag="ACHU2L_000114"
     CDS             6852..8834
                     /gene="fhuB"
                     /locus_tag="ACHU2L_000114"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR1355154.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8869..10149)
                     /gene="hemL"
                     /locus_tag="ACHU2L_000115"
     CDS             complement(8869..10149)
                     /gene="hemL"
                     /locus_tag="ACHU2L_000115"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR1355155.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10315..10362
                     /gene="yadX"
                     /locus_tag="ACHU2L_000116"
     CDS             10315..10362
                     /gene="yadX"
                     /locus_tag="ACHU2L_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR1355156.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10374..11795
                     /gene="clcA"
                     /locus_tag="ACHU2L_000117"
     CDS             10374..11795
                     /gene="clcA"
                     /locus_tag="ACHU2L_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR1355157.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11819..11884
                     /gene="yadW"
                     /locus_tag="ACHU2L_000118"
     CDS             11819..11884
                     /gene="yadW"
                     /locus_tag="ACHU2L_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR1355158.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            11877..12221
                     /gene="erpA"
                     /locus_tag="ACHU2L_000119"
     CDS             11877..12221
                     /gene="erpA"
                     /locus_tag="ACHU2L_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR1355159.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12268..12891)
                     /locus_tag="ACHU2L_000120"
     CDS             complement(12268..12891)
                     /locus_tag="ACHU2L_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR1355160.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12929..13729)
                     /gene="btuF"
                     /locus_tag="ACHU2L_000121"
     CDS             complement(12929..13729)
                     /gene="btuF"
                     /locus_tag="ACHU2L_000121"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR1355161.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13722..14420)
                     /gene="mtnN"
                     /locus_tag="ACHU2L_000122"
     CDS             complement(13722..14420)
                     /gene="mtnN"
                     /locus_tag="ACHU2L_000122"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR1355162.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14504..16021
                     /gene="dgt"
                     /locus_tag="ACHU2L_000123"
     CDS             14504..16021
                     /gene="dgt"
                     /locus_tag="ACHU2L_000123"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1355163.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16151..17575
                     /gene="degP"
                     /locus_tag="ACHU2L_000124"
     CDS             16151..17575
                     /gene="degP"
                     /locus_tag="ACHU2L_000124"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR1355164.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17730..18886
                     /gene="cdaR"
                     /locus_tag="ACHU2L_000125"
                     /pseudo
     CDS             17730..18886
                     /gene="cdaR"
                     /locus_tag="ACHU2L_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(18975..19361)
                     /locus_tag="ACHU2L_000126"
     CDS             complement(18975..19361)
                     /locus_tag="ACHU2L_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR1355165.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19675..20499)
                     /gene="dapD"
                     /locus_tag="ACHU2L_000127"
     CDS             complement(19675..20499)
                     /gene="dapD"
                     /locus_tag="ACHU2L_000127"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR1355166.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20530..23202)
                     /gene="glnD"
                     /locus_tag="ACHU2L_000128"
     CDS             complement(20530..23202)
                     /gene="glnD"
                     /locus_tag="ACHU2L_000128"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR1355167.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23264..24058)
                     /gene="map"
                     /locus_tag="ACHU2L_000129"
     CDS             complement(23264..24058)
                     /gene="map"
                     /locus_tag="ACHU2L_000129"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR1355168.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24426..25151
                     /gene="rpsB"
                     /locus_tag="ACHU2L_000130"
     CDS             24426..25151
                     /gene="rpsB"
                     /locus_tag="ACHU2L_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR1355169.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25409..26260
                     /gene="tsf"
                     /locus_tag="ACHU2L_000131"
     CDS             25409..26260
                     /gene="tsf"
                     /locus_tag="ACHU2L_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR1355170.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26407..27132
                     /gene="pyrH"
                     /locus_tag="ACHU2L_000132"
     CDS             26407..27132
                     /gene="pyrH"
                     /locus_tag="ACHU2L_000132"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR1355171.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27424..27981
                     /gene="frr"
                     /locus_tag="ACHU2L_000133"
     CDS             27424..27981
                     /gene="frr"
                     /locus_tag="ACHU2L_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR1355172.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28073..29269
                     /gene="ispC"
                     /locus_tag="ACHU2L_000134"
     CDS             28073..29269
                     /gene="ispC"
                     /locus_tag="ACHU2L_000134"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR1355173.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            29458..30216
                     /gene="ispU"
                     /locus_tag="ACHU2L_000135"
                     /gene_synonym="uppS"
     CDS             29458..30216
                     /gene="ispU"
                     /locus_tag="ACHU2L_000135"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR1355174.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30229..31086
                     /gene="cdsA"
                     /locus_tag="ACHU2L_000136"
     CDS             30229..31086
                     /gene="cdsA"
                     /locus_tag="ACHU2L_000136"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1355175.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31098..32450
                     /gene="rseP"
                     /locus_tag="ACHU2L_000137"
     CDS             31098..32450
                     /gene="rseP"
                     /locus_tag="ACHU2L_000137"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR1355176.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32480..34912
                     /gene="bamA"
                     /locus_tag="ACHU2L_000138"
     CDS             32480..34912
                     /gene="bamA"
                     /locus_tag="ACHU2L_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR1355177.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35034..35519
                     /gene="skp"
                     /locus_tag="ACHU2L_000139"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35034..35519
                     /gene="skp"
                     /locus_tag="ACHU2L_000139"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR1355178.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35523..36548
                     /gene="lpxD"
                     /locus_tag="ACHU2L_000140"
     CDS             35523..36548
                     /gene="lpxD"
                     /locus_tag="ACHU2L_000140"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR1355179.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36653..37108
                     /gene="fabZ"
                     /locus_tag="ACHU2L_000141"
     CDS             36653..37108
                     /gene="fabZ"
                     /locus_tag="ACHU2L_000141"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR1355180.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37112..37900
                     /gene="lpxA"
                     /locus_tag="ACHU2L_000142"
     CDS             37112..37900
                     /gene="lpxA"
                     /locus_tag="ACHU2L_000142"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR1355181.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37900..39048
                     /gene="lpxB"
                     /locus_tag="ACHU2L_000143"
     CDS             37900..39048
                     /gene="lpxB"
                     /locus_tag="ACHU2L_000143"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR1355182.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39045..39641
                     /gene="rnhB"
                     /locus_tag="ACHU2L_000144"
     CDS             39045..39641
                     /gene="rnhB"
                     /locus_tag="ACHU2L_000144"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR1355183.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            39678..43160
                     /gene="dnaE"
                     /locus_tag="ACHU2L_000145"
     CDS             39678..43160
                     /gene="dnaE"
                     /locus_tag="ACHU2L_000145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1355184.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43173..44132
                     /gene="accA"
                     /locus_tag="ACHU2L_000146"
     CDS             43173..44132
                     /gene="accA"
                     /locus_tag="ACHU2L_000146"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR1355185.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44231..46372
                     /gene="ldcC"
                     /locus_tag="ACHU2L_000147"
     CDS             44231..46372
                     /gene="ldcC"
                     /locus_tag="ACHU2L_000147"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1355186.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46429..46818
                     /locus_tag="ACHU2L_000148"
     CDS             46429..46818
                     /locus_tag="ACHU2L_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1355187.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46883..48181
                     /gene="tilS"
                     /locus_tag="ACHU2L_000149"
     CDS             46883..48181
                     /gene="tilS"
                     /locus_tag="ACHU2L_000149"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR1355188.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48230..48490)
                     /gene="rof"
                     /locus_tag="ACHU2L_000150"
     CDS             complement(48230..48490)
                     /gene="rof"
                     /locus_tag="ACHU2L_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR1355189.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48477..48677)
                     /locus_tag="ACHU2L_000151"
     CDS             complement(48477..48677)
                     /locus_tag="ACHU2L_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR1355190.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48843..49388
                     /locus_tag="ACHU2L_000152"
     CDS             48843..49388
                     /locus_tag="ACHU2L_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR1355191.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49385..49807
                     /gene="arfB"
                     /locus_tag="ACHU2L_000153"
     CDS             49385..49807
                     /gene="arfB"
                     /locus_tag="ACHU2L_000153"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR1355192.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49821..50531
                     /gene="nlpE"
                     /locus_tag="ACHU2L_000154"
                     /gene_synonym="cutF"
     CDS             49821..50531
                     /gene="nlpE"
                     /locus_tag="ACHU2L_000154"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR1355193.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(50686..51510)
                     /locus_tag="ACHU2L_000155"
     CDS             complement(50686..51510)
                     /locus_tag="ACHU2L_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR1355194.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(51563..53281)
                     /gene="proS"
                     /locus_tag="ACHU2L_000156"
     CDS             complement(51563..53281)
                     /gene="proS"
                     /locus_tag="ACHU2L_000156"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR1355195.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(53392..54099)
                     /gene="tsaA"
                     /locus_tag="ACHU2L_000157"
     CDS             complement(53392..54099)
                     /gene="tsaA"
                     /locus_tag="ACHU2L_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR1355196.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(54096..54500)
                     /gene="rcsF"
                     /locus_tag="ACHU2L_000158"
     CDS             complement(54096..54500)
                     /gene="rcsF"
                     /locus_tag="ACHU2L_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR1355197.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(54618..55433)
                     /gene="metQ"
                     /locus_tag="ACHU2L_000159"
     CDS             complement(54618..55433)
                     /gene="metQ"
                     /locus_tag="ACHU2L_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR1355198.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(55473..56126)
                     /locus_tag="ACHU2L_000160"
     CDS             complement(55473..56126)
                     /locus_tag="ACHU2L_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR1355199.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(56119..57150)
                     /gene="metN"
                     /locus_tag="ACHU2L_000161"
     CDS             complement(56119..57150)
                     /gene="metN"
                     /locus_tag="ACHU2L_000161"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR1355200.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57338..57913
                     /gene="gmhB"
                     /locus_tag="ACHU2L_000162"
     CDS             57338..57913
                     /gene="gmhB"
                     /locus_tag="ACHU2L_000162"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR1355201.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            58277..>58547
                     /locus_tag="ACHU2L_000163"
     rRNA            58277..>58547
                     /locus_tag="ACHU2L_000163"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        13917 a        14417 c        16100 g        14113 t
ORIGIN      
        1 cctctccctc aagggagagg ggaccgatcg agcgcaaatt ttgagagata tcttcttctg
       61 tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca
      121 tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt ccggtcaaac
      181 aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat gatgactatg
      241 aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg
      301 gcaaacgcgg ctggctatgg ctactgctaa aactggctat cgtttttgcc gtgctgatcg
      361 ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc aaggtctggc
      421 aactgcccgc ggcagtttat ggccgaatgg tcaaccttga accagatatg accatcagca
      481 agaacgaaat ggtgaagctg ctggaggcaa cacagtatcg tcaggtgtcg aaaatgaccc
      541 gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt ccgtttgatt
      601 tcccggacag taaagaaggg caggtgcgtg cgcgtctgac ctttgatggt gatcatctgg
      661 cgaccatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt gatccgcgtc
      721 tgatcaccat gatctcctcg ccaaacggtg agcagcgttt gtttgtgccg cgcagtggtt
      781 tccctgattt gctggtggat actttgctgg cgacagaaga ccgtcatttt tacgagcatg
      841 atggaatcag tctctactcc attgggcgtg cggtgctggc aaacctgacc gcaggtcgca
      901 cggtgcaggg tgcgagtacg ctgacgcaac agctggtgaa aaacctgttc ctctccagcg
      961 agcgttctta ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg gacgcgcgtt
     1021 acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt cagagcggcg
     1081 acaacgaaat ccgcggcttc ccgctggcaa gcttgtatta ctttggtcgc ccggtagaag
     1141 agctaagcct cgaccagcag gcgctgttag tcggtatggt gaaaggggcg tccatctaca
     1201 acccgtggcg taacccgaaa ctggcgctgg agcgacgtaa tctggtgctg cgtctgctgc
     1261 aacagcaaca gattattgat caagaactct atgacatgtt gagtgcccgt ccgctggggg
     1321 ttcagccgcg tggtggggtg atctctcctc agccagcctt tatgcagctg gtgcgtcagg
     1381 agttgcaggc aaaactgggc gataaggtaa aagatctctc cggcgtgaag atcttcacta
     1441 cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc attccggcac
     1501 tgaagaaaca gcgtaagttg agcgatcttg aaactgcgat tgtggtcgtc gaccgcttta
     1561 gtggtgaagt tcgtgcgatg gtcggaggtt ctgagccgca gtttgcgggc tacaaccgtg
     1621 cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat ctgacggctt
     1681 taagccagcc gaaaatctat cgcctgaaca cgtggattgc cgatgcgcca atcgcgctgc
     1741 gtcagccgaa tggccaggtc tggtcaccgc agaacgatga ccgtcgttat agcgaaagcg
     1801 gcagagtgat gctggtggat gcgttgacac gttcgatgaa cgtgccgacg gtaaatctgg
     1861 ggatggcgct ggggctgcct gcggttacgg agacctggat caaactgggc gtaccgaaag
     1921 atcagttgca tccggttccg gcaatgctgc tgggggcgtt gaacttaacg ccaatcgaag
     1981 tggcgcaggc attccagacc atcgccagcg gtggtaaccg tgcaccgctt tctgcactgc
     2041 gttcggtaat cgcggaagat ggtaaggtgc tgtatcagag cttcccgcag gcagaacgcg
     2101 ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg gtacaacgtg
     2161 gtacgggtcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg aaaacaggga
     2221 ctaccaacaa taacgtagat acctggtttg caggcattga cggcagcacg gtgaccatca
     2281 cctgggtagg ccgtgataac aaccagccga ccaaactgta tggagccagc ggggcaatgt
     2341 cgatttatca gcgttatctg gctaaccaga cgccaacgcc gctgaatctt gttccgccag
     2401 aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc ggtggcatgc
     2461 gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagc gagatgcagc
     2521 agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag ccgcaacagc
     2581 aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatc aaggatatgt
     2641 ttggtagtaa ttaacatcta agcgtgaaat accggatggc gagttgccat ccggtaaaat
     2701 aacatcccat ctaagatatt aaccctttct tttcatctgg ttgtttatta acccttcagg
     2761 aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta tcttatcttt
     2821 ataataatca ttctcgttta cgttattatt cactttacat cagagatata ccaatggcgc
     2881 gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta gtagccacag
     2941 cggttagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa gacactatca
     3001 ccgttaccgc tgcacctgcg ccgcaagaaa gcgcatgggg gcctgctgca actattgcgg
     3061 cgcggcagtc agctaccggc actaaaaccg atacgccgat tcaaaaagtg ccacagtcta
     3121 tttctgttgt gaccgccgaa gagatggcgc tgcatcagcc gaagtcggta aaagaagcgc
     3181 ttagctacac gccgggtgtc tctgttggta cgcgtggcgc atccaacacc tatgaccacc
     3241 tgatcattcg cggttttgcg gcagaaggcc aaagccaaaa taactatctg aatggcctga
     3301 agttgcaggg caacttctat aacgatgcgg tcattgaccc gtatatgctg gaacgcgctg
     3361 aaattatgcg tggcccggtt tccgtgcttt acggtaaaag cagtcctggc ggtctgttga
     3421 atatggtcag caagcgtccg accaccgaac cgctgaaaga agttcagttt aaagccggta
     3481 ctgacagcct gttccagact ggttttgact ttagcgatgc gttggatgat gacggtgttt
     3541 actcttatcg cctgaccggt cttgcgcgtt ctgccaatgc ccagcagaaa gggtcagaag
     3601 agcagcgtta tgctattgca ccggcgttca cctggcgtcc ggatgataaa accaatttca
     3661 ccttcctttc ttacttccag aacgagccgg aaaccggtta ttacggctgg ttgccgaaag
     3721 agggaaccgt tgagccgctg ccgaacggta agcgtctgcc gacagacttt aatgaagggg
     3781 cgaagaacaa cacctattct cgtaatgaga agatggtcgg ctacagcttc gatcacgaat
     3841 ttaacgacac ctttactgtg cgtcagaacc tgcgctttgc tgaaaacaaa acctcgcaaa
     3901 acagcgttta tggttacggc gtctgctccg atccggcgaa tgcttacagc aaacagtgtg
     3961 cggcattagc gccagcggat aaaggccatt atctggcacg taaatacgtc gttgatgatg
     4021 agaagctgca aaacttctcc gttgataccc agttgcagag caagtttgcc actggcgata
     4081 tcgaccacac cctgctgacc ggtgtcgact ttatgcgtat gcgtaatgac atcaacgcct
     4141 ggtttggtta cgacgactct gtgccactgt tcaatctgta caatccggtg aatcccgatt
     4201 tcgacttcaa tgccaaagat ccggcaaact ccggccctta ccgcattctg aataaacaga
     4261 aacaaacggg cgtttatgtt caggatcagg cgcagtggga taaagtgctg gtcaccctag
     4321 gcggtcgtta tgactgggca gatcaagaat ctcttaaccg cgttgctggg acgaccgata
     4381 aacgtgatga caaacagttt acctggcgtg gtggtgttaa ctacctgttt gataatggtg
     4441 taacacctta cttcagctat agcgaatcgt ttgaaccttc ttcgcaagtt gggaaggatg
     4501 gtaatatttt cgcaccgtct aaaggtaagc agtatgaagt cggcgtgaaa tatgtaccgg
     4561 aagatcgtcc gattgtagtt actggtgccg tgtataatct cactaaaacc aacaacctga
     4621 tggcggaccc tgagggttcc ttcttctcgg ttgaaggtgg cgagatccgc gcacgtggcg
     4681 tagaaatcga agcgaaagcg gcgctgtcgg cgagtgttaa cgtagtcggt tcttatactt
     4741 acaccgatgc ggaatacacc accgatacta cctataaagg caatacgcct gcacaggtgc
     4801 caaaacacat ggcttcgctg tgggctgact ataccttctt tgacggtccg ctttcaggtc
     4861 tgacgctggg caccggtggt cgttatactg gctccagcta tggtgatccg gctaactcct
     4921 ttaaagtggg aagttatacg gtcgtggatg cgttagtgcg ttatgatctg gcgcgagtcg
     4981 gcatggcggg ctccaacgtg gcgctgcatg ttaacaacct gttcgatcgt gaatacgtcg
     5041 ccagctgctt taacacttat ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg
     5101 caacctttcg tttctaattt ctcttttggg gcacggattt ccgtgcccat ttcacaagtt
     5161 ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc gtaatatctc
     5221 ctttcgtgtg cccgggcgca cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa
     5281 agtgaccggt ctgattggtc acaacggttc tggtaaatcc actctgctca aaatgcttgg
     5341 tcgtcatcag cctccgtcag aaggggagat tcttcttgat gcccagccgc tggaaagctg
     5401 gagcagcaaa gcgtttgccc gcaaagtggc ttatttgccg cagcagcttc ctccggcaga
     5461 agggatgacc gtgcgtgaac tggtggcgat tggtcgttac ccgtggcatg gcgcgctggg
     5521 gcgctttggt gcggcagatc gcgaaaaggt cgaggaagct atctcgctgg ttggcttaaa
     5581 accgctggcg catcggctgg tcgatagtct ctctggcggc gaacgtcagc gggcgtggat
     5641 cgccatgctg gtggcgcagg atagccgttg tctgttgctc gacgaaccga cttcagcgct
     5701 ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc aggaacgtgg
     5761 cctgacggtc attgccgtgt tgcacgatat caatatggcg gcacgctact gtgattatct
     5821 ggtcgccctg cgcagtggtg aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg
     5881 cgaaaccctc gaaatgattt atggcatccc gatgggtatt ttgccgcatc cggcgggtgc
     5941 tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg ccgtcgactg
     6001 ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca cgcggcggct
     6061 attgacccca atcgtattgt ggcgctggag tggttgccgg tggaattact gctggcgctc
     6121 ggcatcgtgc cttacggcgt ggcggatacc atcaactatc gcctgtgggt cagcgaacca
     6181 ccattgccgg actcagtgat tgacgtcggt ttgcgtacag aacctaacct tgaactgctg
     6241 accgaaatga aaccgtcgtt tatggtctgg tcggcaggat atggcccttc atcagaaatg
     6301 ctggcacgta ttgcgccggg tcgcggattt aacttcagtg acggcaaaca tccgttggcg
     6361 atggcgcgta aatcgctgac ggaaatggca gatttactta acctgcaaag cgcagcggaa
     6421 acgcatttag cgcaatacga agactttatc cgcagcatga aaccccgctt tgtgaagcgt
     6481 ggtgcacgcc cgttactgct gacgacgctt atcgatccgc gccatatgct ggtcttcggt
     6541 ccaaacagct tgttccagga aattctcgat gagtacggca tcccaaatgc ctggcaaggg
     6601 gaaaccaact tctggggcag taccgccgtc agtatcgatc gtctggcagc gtataaagac
     6661 gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct aatggcaacc
     6721 ccgctgtggc aggccatgcc gtttgtccgt acagggcgct ttcagcgcgt acccgcagtc
     6781 tggttttatg gtgcaacgct ttcggcaatg cactttgtgc gtgttctgga taacgccatc
     6841 ggaggtaaag cgtgagtaaa cgaattgcgc ttttcccggc gttattgctg gcgctgttag
     6901 tgattgtcgc tacggcgctc acctggatga acttctcaca ggcgctgccg cgcagtcagt
     6961 gggcgcaggc cgcctggtcg ccggatattg acgtcatcga gcagatgatt tttcactaca
     7021 gcttgttgcc gcgtctggcg atttcgctgc tggtgggcgc gggcctgggg ctggtgggcg
     7081 tgctgtttca gcaagtactg cgtaacccgc tggcggaacc gacgacgttg ggcgttgcaa
     7141 caggcgcgca actggggatt accgtcacca cgctctgggc gatccctagc gcgatggcga
     7201 gccagtttgc tgcgctggca ggggcttgcg ttgttggctt aattgtcttt ggcgtcgcgt
     7261 gggggaaacg gctgtcgccg gtaacgctga ttttcgcggg gttggtagtg agcctttatt
     7321 gcggcgcaat caatcagtta ctggttatct tccatcatga ccagttgcaa agcatgtttc
     7381 tgtggagcac tggaacgctg acgcaaaccg actggggcgg tgttgagcgt ttatggccgc
     7441 agttgctggg cggtgtgatg ctgacgttgc tgctacttcg tccgttaacc ctgatggggc
     7501 ttgatgatgg cgtggcgcgc aatctcgggc tggccttgtc gcttgcgcgt ctggcggcgc
     7561 tgtcgctggc gattgtcatc agtgcgctgc tggtgaacgc tgtggggatt atcggcttta
     7621 tcgggttgtt cgcgccgctg ctggcaaaaa tgctgggggc gcggcgtctg ctgccacgac
     7681 tgatgctggc gtcgttgatt ggtgcgctga tcctctggct ttccgatcaa atcatcctct
     7741 ggctgactcg cgtgtggatg gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc
     7801 cgctgctact gtggctgctg ccgcgtttac gcagcattag cgcgccggat atgaaggtta
     7861 acgatcgtgt cgcgactgaa cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc
     7921 tgttgttgat ggctgtggtg gtggcgctgt cgtttggtcg tgatgcgcac ggctggacgt
     7981 gggcgagcgg ggcgttgctc gaggatttaa tgccctggcg ctggccgcga attatggcgg
     8041 cgctgtttgc gggcgtcatg ctggcggtag cgggctgtat tattcagcga ctgaccggca
     8101 acccgatggc aagcccggaa gtgctgggga ttagctccgg cgcggcgttt ggtgtggtgt
     8161 taatgctgtt tctggtaccg ggtaatgcct ttggctggtt gttacccgca ggcagtctcg
     8221 gggcggcggt gacgctgttg atcattatga tcgccgccgg acgcggtgga ttttccccac
     8281 accgcatgtt actggcgggg atggcgttaa gtaccgcgtt caccatgctt ttgatgatgt
     8341 tgcaggcaag tggtgacccg cgaatggcgc aagtgctgac ctggatttcc ggttcgacct
     8401 acaacgcgac cgatgctcag gtctggcgca ccggaattgt gatggtgatt ttgctggcga
     8461 ttactccgct gtgccgccgc tggctgacca ttttaccgct gggtggtgat accgcgcggg
     8521 cggtagggat ggcgctgacg ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga
     8581 cggcgaccgc gacgatgact attggaccgt tgagttttgt tggtttaatg gcaccgcata
     8641 ttgcgcggat gatgggcttt cgacggacga tgccacacat cgtaatttcg gcgctggtgg
     8701 gtggtttact gctggtgttc gctgactggt gtgggcggat ggtgctgttt ccattccaga
     8761 tcccggcggg gctgctgtcg acctttatcg gtgcgccata ttttatctat ttgctgagaa
     8821 agcagagccg ttaatagaat gcctcgcccg gtgggcgagg caaaattatc acaacttcgc
     8881 gaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt gcgccacaga
     8941 cataaagcct gcttcaaacg ctgacggtgc caggtaaaca ccttcatcca gcatcatatg
     9001 gaagaaacgc ttaaaacgct cgacgtcaca ggccatcaca tcctgatagc acgtcacgga
     9061 ctcggcgtcg gtaaagaaga taccgaacat accaccgacg tggttaacga ccagcggaat
     9121 tcccgcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca gctcatccag
     9181 cgtttcgtga atgcccggct gcgcgacttc attcagacag gcgaaacccg ctgccatcgc
     9241 aatcgggtta ccggaaagcg tacccgcctg atagaccgga cctgtcgggg ccagcgcatc
     9301 cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga tgatttttcc
     9361 gaggcaggtt aaatctggca ctacggcgaa ataatcctgt gcgccagcta gcgctacgcg
     9421 gaaaccggtc atcacttcat cgatgatcag caacgcgcca aattcgtcgc acagcgcacg
     9481 cagacctggc aggaactctg gcagcggcgg aacacagttc atattgcctg ccaccggctc
     9541 gacgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta cagaggccag
     9601 atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacgc ccggcgagtt
     9661 tggctggcct aacgtgagtg cgccagaacc ggctttcacc agcaggcagt cagcgtgacc
     9721 gtggtaacaa ccttcaaatt taatgatttt gtcgcgaccg gtaaaaccac gggccaggcg
     9781 gatggcgctc atcgtcgcct cggtgccgga gttcaccatg cgcaccatat ccatggtcgg
     9841 gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggtcggtg caccaaagct
     9901 taaaccacgc tcggcggctt caatcacggc attgcggatc gccggatggt tatggcccag
     9961 caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa catcgtacag
    10021 ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag taaaggcgcg
    10081 aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa gattttcaga
    10141 cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt ccatgttatt
    10201 cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa tcctgatttc
    10261 ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat tttcatggaa
    10321 cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat tggatgaaaa
    10381 ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga caactgattc
    10441 gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg
    10501 gcacgcttgt cgggctggct gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc
    10561 aacgtatggg ggcgctggta catactgctg ataattatcc gctactgtta accgtcgctt
    10621 ttctctgttc ggcggtgctg gcgatgttcg gctacttttt ggtgcgcaaa tacgcgcccg
    10681 aagcaggggg atcggggatc ccggaaattg aaggggcgct ggaagatcaa cgtcctgttc
    10741 gctggtggcg tgtattgccg gtgaaattct ttggcgggct ggggacactc ggcggaggca
    10801 tggggctagg gcgcgaagga ccaaccgtgc agatcggcgg caatatcagt cgcatggtgc
    10861 ttgatatttt ccgcctgaaa ggtgatgaag ctcgccatac gctgctggca accggtgctg
    10921 ctgcggggct ggctgcggcc tttaacgcgc cgctggcggg tattttgttt attatcgaag
    10981 agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt attggcgtca
    11041 ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt gacgtcggta
    11101 aactttctga tgcgccgctt aatacgctgt ggctttatct gatcctcggt attatttttg
    11161 gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg ctgcaccgtg
    11221 tgcacggcgg caatattacc aaatgggtac tgatgggggg ggcgattggc ggtctgtgtg
    11281 gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg attcctatcg
    11341 ctaccgcggg gaatttcagc atgggaatgc tggtgtttat cttcgtcgct cgggtcatta
    11401 ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg atgctggcgc
    11461 tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt ccgcaatatc
    11521 acctggaggc ggggacgttt gctattgccg gaatgggggc attactggcg gcatctattc
    11581 gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac cagctcattt
    11641 tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc ggcgggaaac
    11701 ctctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc
    11761 gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt attagataat
    11821 ggcgattatt attgggttag aatttgcccg attgccgatg tcgtttggag caaaatatga
    11881 gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa gttaaaagcc
    11941 tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcacc ggtggcggtt
    12001 gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc gatatgacca
    12061 tcgaaaaaca gggcgttggc ctggtggttg atccgatgag cctgcaatat ctggtcggcg
    12121 gttccgttga ctacaccgaa ggactggaag gttctcgttt catcgtgacc aacccgaacg
    12181 cgaaaagcac ctgcggttgc ggttcttcct ttagcatcta atctgttgtt catgtgccgg
    12241 ataaactatc cggcacatct tctctcttca acgcccattc tcatccagcg caaacgtcgg
    12301 tagcttaaga tgccaacgaa tagccgccag ccgaatcaat agcgtcacga ccatgcccat
    12361 catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc
    12421 gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga tttcgcggac
    12481 cagtacatca cgaatgatcc cgccgccaac gccagtaatg acgcccatac aaaccgcgat
    12541 taacggaccg gcttccgcat taaaggcttt attcacgcta atgccgacaa acaccgccag
    12601 accaacggcg tccagcaccg gcaacatcca ttttggtaag cgtcttggct ggcgcaccag
    12661 cacgatggtc agcatgctgg tgaccatggc aacgaccaga tcggtgggat ctttcaccca
    12721 aaataccggg ccgtgatcca gcgccatgtc gcgaattgtt ccgtcgccta ctgcggtaac
    12781 cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta acaaaacgcc
    12841 gtagatggca aataccgctg tgccgactat atccagccaa tagacgagca ttgttcaatc
    12901 cccactgagc acctgaaaag gtcaggcact aatctacctg tgaaagcgca ttacagagct
    12961 gttgtgctgc gaggataata cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa
    13021 taacgggaat tttgagctgt tcaccccagt attgtttgat tttaggaatt tggtccggtc
    13081 cgcctgtaat gacaatagcc tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg
    13141 gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg agtacctggt
    13201 tctgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc agaaaaacac
    13261 gttttttagg tttatcagca tattgcgctt tcaattgcac gtactgatcc agcagggatt
    13321 gcgcggcttg ttcggccttg tctggttgcg gactccaggg ggccagttga cgtaacgcat
    13381 tggcaatttg ttcaatgctt gtcgcatcga cccacatcac ttttattccc agcgaagcca
    13441 gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga tcgggtttca
    13501 gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aacctgctca atcttttgcg
    13561 cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg
    13621 caagttcagt gttggcggga gaaagcgtga tgacgcgcgg cgcggcgttg agccacagtg
    13681 gcgcgagaaa agacagggcg accagcgccc tgaacagtga tttagccatg tgccagtttc
    13741 tgcaccagtg actcaaccat caggctggac tgtttagcgg caacagccag gaactcatcg
    13801 aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac aacaaacggg
    13861 acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac agcaatggcc
    13921 tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg
    13981 ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc ctcagcggca
    14041 gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg accgtattca
    14101 taaccaaatg ccgtgacatc cgcgtcgtga taacgcgctt cgtccgagac aacgatatcg
    14161 cccactttca gcgttggtgc caggccaccg gcagaaccgg tgttaataat cacatctggc
    14221 ttgcagtgtt ccaacagcag agtggcaccc agcgccgcag cgactttacc gatgcccgat
    14281 ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca accgccgaga
    14341 ctgatagttt gacggttttc gattttgtca cgcagcagcg taacttcttc ttccattgca
    14401 ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaag gcatagttta
    14461 ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac agattgattt
    14521 ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca
    14581 tgagatcctg cggatcttcg agagcgatcg cgggcgtatc atcaactctc cggcaattcg
    14641 tcgtctgcaa caaaagaccc aggtttttcc actggagcgc aatgccgccg tgcgcacgcg
    14701 tcttacccac tcgatggaag tccagcaggt gggacgctac atcgccaaag aaattttaag
    14761 ccgtctgaaa gagcttaaat tgctggaagc atacggcctg gatgaactga ccggcccctt
    14821 tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc cgtttggtca
    14881 ttttggcgaa gcagcgataa atgactggtt tcgccaacgt ttgcacccgg aagatgccga
    14941 aagccagcct ctgactgacg atcgctgcag cgtggcggca ctacgtttac gggacgggga
    15001 agaaccgctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa
    15061 tgcacaaggc attcgtctgg tgcatacatt gatgcggatg aatctcactt gggcacaggt
    15121 tggcggtatt ttaaaatata cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca
    15181 tcactattta atgaaaaagc cgggttatta tctttctgaa gaagcctata ttgcccgctt
    15241 gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacgtgga ttatggaagc
    15301 tgccgacgac atctcctatt gtgtggcaga ccttgaagat gcggtagaga aaagaatatt
    15361 taccgttgag cagctttatc atcatttgca cgaagcgtgg ggccagcatg agaaaggttc
    15421 gctcttttcg ctggtggttg aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg
    15481 cagtacggaa gatcagtttt ttatgtattt acgggtaaac accctaaata aactggtacc
    15541 ctacgcggca caacgattta ttgataatct gcctgcgatt ttcgccggaa cgtttaatca
    15601 tgcattattg gaagatgcca gcgaatgcag cgatcttctt aagctatata aaaatgtcgc
    15661 tgtaaaacat gtgtttagcc atccggatgt cgagcagctt gaattgcagg gctatcgggt
    15721 cattagcgga ttattagaga tttatcgtcc tttattaagc ctgtcgttat cagactttac
    15781 tgaactggta gaaaaagaac gggtgaaacg tttccctatt gagtcgcgct tattccacaa
    15841 actctcgacg cgccatcggc tggcctatgt cgaggctgtc agtaaattac cgtcagattc
    15901 tcctgagttt ccgctatggg aatattatta ccgttgccgc ctgctgcagg attatatcag
    15961 cggtatgacc gacctctatg cgtgggatga ataccgacgt ctgatggccg tagaacaata
    16021 accagtcttt tgtaaagacg aacaataaat ttttaccttt tgcagaaact ttagttcgga
    16081 acttcgggct ataaaacgaa tctgaagaac acagcaattt tgcgttatct gttaatcgag
    16141 actgaaatac atgaaaaaaa acacattagc actgagtgca ctggctctga gtttaggttt
    16201 ggcgttatct ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca
    16261 gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt
    16321 agaaggtagc acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg
    16381 tgatgattct ccgttctgcc aggaaggttc tccgttccag agctctccgt tctgccaggg
    16441 gggccagggc ggtaatggcg gcggccagca acagaaattc atggcgctgg gttccggcgt
    16501 catcattgat gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac
    16561 ggtgattaaa gtccagctga gcgatggtcg taagttcgac gcgaagatgg ttggcaaaga
    16621 tccgcgctct gatatcgcgc tgatccaaat ccagaacccg aaaaaactga ccgcaattaa
    16681 gatggcggat tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt
    16741 tggtctgggc gagacggtaa cttccgggat tgtctctgcg ctggggcgta gcggcctgaa
    16801 tgctgaaaac tacgaaaact tcatccagac cgatgcagcg atcaaccgtg gtaactccgg
    16861 tggtgcgctg gttaacctga acggcgaact gatcggtatc aacaccgcga tcctcgcacc
    16921 ggacggcggc aacatcggta tcggttttgc tatcccgagt aacatggtga aaaacctgac
    16981 ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga
    17041 gctgaattcc gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag
    17101 ccaggttctg cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac
    17161 ctcactgaac ggtaagccga tcagcagctt tgccgcactg cgtgctcagg tgggtactat
    17221 gccggtgggc agcaaactga ccctgggctt actgcgcgac ggtaagcagg ttaacgtgaa
    17281 cctggaactg cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat
    17341 tgaaggcgct gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa
    17401 gacgggcact ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggtgcgaa
    17461 ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt
    17521 gctggcactg aacattcagc gcggcgacag caccatctac ctgttaatgc agtaatctcc
    17581 ctcaacccct tcctgaatac gggaaggggt tctccttaca atctgtgaac ttcgccacaa
    17641 ctccatacat cttcatcatc ctttgggcat ttgcacaatg ccgtacgtta cgtacttcct
    17701 tatgctaagc cgtgcataac ggaggactta tggctggctg gcatcttgat accaaaatgg
    17761 cgcaggatat cgtggcacgt accatgcgca tcatcgatac caatatcaac gtaatggatg
    17821 cccgcgggcg aattatcggc agcggcgatc gtgagcgtat tggtgaattg cacgaaggtg
    17881 cattgctggt actttcacag ggacgagtcg tcgatatcga tgacgcggta gcacgtcatc
    17941 tgcacggtgt gcggcagggg attaatctac cgttacggct ggaaggtgaa attgtcggcg
    18001 taattggcct gacaggtgaa ccagagaatc tgcgtaaata tggcgaactg gtctgcatga
    18061 cggctgaaat gatgctggaa cagtcgcggt tgatgcactt gttggcgcag gatagccgtt
    18121 tgcgggaaga actggtgatg aacctgattc aggcagagga gaatactccc gcacttctga
    18181 atgggcgcaa cggctgggga tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt
    18241 cgacagcggt cagcttggcg tggacagcgc aatggcggag ttacaacaac tgcaaaacgc
    18301 gctgactacg cccgagcgta ataatctggt ggcgattgtc tcgctaaccg aaatggtggt
    18361 gttgaaaccg gcgttgaact cttttgggcg ctgggatgca gaagatcatc gtaagcgagt
    18421 tgaacaactg attacccgca tgaaagagta cggccagctg cgttttcgcg tttcactggg
    18481 caactatttt accggtcctg gcagtattgc ccgatcctat cgtacggcga aaacgacgat
    18541 ggtggtgggt aaacagcgga tgccagaaag tcgctgctat ttttatcagg atctgatgtt
    18601 acctgtgtta ctcgacagtt tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct
    18661 ggcgcggctg aaagcgatgg acaataacgg cttgctgcga cgaacgctgg cggcgtggtt
    18721 tcgccacaat gtgcaaccgc tggcaacgtc aaaggcgttg tttattcatc gtaataccct
    18781 ggagtatcgg cttaatcgta tatcggaact gaccgggctt gatttgggca attttgatga
    18841 caggttgctg ctgtatgtgg cgttacaact ggatgaagag cggtagatta tgcgttaagg
    18901 ttgaggcggc gctggctcat tcccctcagc ccgaagggga gaggggaatg cccgagttaa
    18961 ccgtcaattc gggattattt acgcgttagt ttttccagat cggcttcaat ctcgctgatc
    19021 ttattggtta cgactgactc caggtgacgt aagtcgtcga ggatcttacg cttaagatca
    19081 acttcgctgc ggtcacgctg acagatttga tcaagctcat caatgatata ccgtagattc
    19141 gggctgattt cctggacttc tttataaccc tgacccacac catcagcgac gaccgtttta
    19201 cgctgacgcg gatatttaaa cttaacgctc ttggcgaaaa actcgccttt gtctttctgg
    19261 aaatagattt tcagaatatc gttgttggct tcctgccgga ggctgtaacg atcaatttct
    19321 tcaggattgg taatacccag acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag
    19381 tcagtaaatt gtgcttattt tagcatttgg cttggcccgc ccggctcgac tgtgatcgca
    19441 gaaagccttt ttcttccaga cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta
    19501 ttgtggcgat attccatggg atggattagc acatttatct tcgttttgcg aattacatga
    19561 cagtccgaaa caagatgaaa aagtatttaa aaaaggggga ggaagaatga gtaggacgaa
    19621 ttattctgag ccagaaagca tacgggcagc atcatgctgc ccgtatatgt actcttaatc
    19681 gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt caactttctt
    19741 aacgataacc gcacagtaga ggctgtattt gccatctttt gacggcagat tacctgaaac
    19801 aaccacagac cccgccggaa cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat
    19861 acgggtgctc tgaccaatgt atacgcccat agagatgacg gaaccttctt cgacaatcac
    19921 cccttcaacc acttcagagc gtgcgccaat gaagcagtta tcttcaataa tggtcgggtt
    19981 agcctgcagc ggctccagca cgccgccgat gcccacgcca ccggaaaggt ggacgttttt
    20041 accaatctgc gcacaagaac cgacggtcgc ccaggtatca accatggtgc cttcatcaac
    20101 atatgcgccg atgttaacgt aagacggcat cagcacggtg ttacgggcaa taaacgcacc
    20161 ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctggaaac gtgcttcgtc
    20221 gtagtcggcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc cttcgatcac
    20281 ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact gatgcgtcac
    20341 ccactgaccg tcaatttttt ccgctacacg cagtgcgccg gaatccagca gggcgatcac
    20401 ctgatttacc gcttcgcggg taacggtgtc tgcattggct ggcgtgatct cggcacggcg
    20461 ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct tttcatatca
    20521 gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct gatgcacttc
    20581 ctgctgcaac tcgttattaa gcgcacgccg gtcagcggtg gcaattatga ataaatcttc
    20641 tactcgctcg ccaatggttg taattcgggc accatgaagc gaaattccca gatcggcaaa
    20701 aattttcccg actcgcgcca gcagtccagg ttggtcgagg gcgatcagtt cgaggaacga
    20761 tttgcggtcg gtatgggtcg gcaaaaacgt tacttcggtt tcaacagtaa aatggcgtaa
    20821 tttggcgggt tggcgacggg gctgcggtgg ctgccagcta ctttgcgtca gcacctgctc
    20881 cagaccaaac cgaataacct catggcgatc tgcggacagc gggctgccat cgggttccag
    20941 cacgataaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac
    21001 acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg ggcggtccgg
    21061 gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca ataccagcgg
    21121 tttgcttaaa tcatgctgta ataaatggcg ggcatgccag gccagttgat ttgggctatg
    21181 gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat
    21241 gttatccatg cgcagtagtg ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat
    21301 atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg
    21361 caacagactt tgcttccagc tattccacag tgtttcgttg gtggcgcaaa tgtcagccac
    21421 tgtcaggcat accagatagc gcagacgatt ttccgtttga acttcttcgg caaactgctt
    21481 gatgacttca gggtcctgaa tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg
    21541 gcgaaccagc caggcaacca gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa
    21601 atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc
    21661 gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc agacgtccac
    21721 acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt tcagcatcac
    21781 gcggatagta tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac
    21841 gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca ttggcaatag
    21901 tccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacagttttc gtgcttgcgg
    21961 aatattacac agcggttgtt gcagatggcg acgggcatgg cgtaactggc gcagcgtggt
    22021 ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac gcaagatggc
    22081 ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg
    22141 aaactcatcg tcgagtggac gtggtttttc gtcggcggga agggcgagga tggcttcatc
    22201 gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc
    22261 cttcatcatc cgctcgaccg gttcgttgcc ttcaccactg taattcagac gctgagcgac
    22321 gctaagttgg cgatcgaata acagacgatt atcgtaacgg ctgacgacca gatgcagggc
    22381 aaagcgaata cgccacagta tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt
    22441 taagaagcca aacccgacca tttcatccag cgatgttgcg ccaaaatgac ggcgggccac
    22501 ccattgcaga gtgtgaatat cgcgcaagcc gccagggcta cttttgatgt ctggttcaag
    22561 gttgtagctg gtgccatggt aacgctgatg gcgctggttc tgttcttcaa ctttcgctgc
    22621 gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca gttcgaggaa
    22681 gagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga cggttaaatc
    22741 cgataaccct tccaacatgc actcttcaag cgtgcgcaca ctatgaccga cttccagctt
    22801 tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt ccgggagctt
    22861 tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta
    22921 gccaccgacg gcaaccaatg ccaggtcggc aatctggctg aatcccgctt caatccataa
    22981 tcgttgcagg agctggtcta tgaactcggt acgtgcctca atcaactgtt ctgcagagat
    23041 cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg cttttatccc
    23101 acagacggtt aattcatcac ggggccaggc gcatggattt tgcggttgac cgggcagggt
    23161 ggagagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc acccataaga
    23221 ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg tcgtgcgaga
    23281 ttatcgccgg gatggtgtca tccttgcgta gcgtcaaaat ttcgcagccg ttatcagtca
    23341 ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt accgtccagc
    23401 catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctca atggtgaacg
    23461 tcatccccgg tttcagcacg acgttggttt cacgggagtc atagtgcagc acctgcggtt
    23521 cttcatggaa gccgcgacca ataccgtgtc cgcaatattc acgaacgacg gagaagcctt
    23581 ctgcttcgac aaatttctga atcgccgcac cgatttcgcg cagattaatg cctggtttta
    23641 ccatgcgtag cgccaggtac aggctttctt gcgtgatgcg gcacaggcgt tcgcccatga
    23701 tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca tctttgatta
    23761 cggtgacatc aatgttaacg atatcgccat ctttcagcag cttagcatcg tccgggatac
    23821 cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg tgatagccga
    23881 ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc attacagatg cgatccagct
    23941 cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact tcggcagcca
    24001 gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag atagccatta
    24061 attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt caatggtatc
    24121 acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg aaggttttct
    24181 ataatagaaa attcgacgtc tgatgctgta cacagcgcca acaattattg gtgtccacga
    24241 cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac agactggacg
    24301 gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc
    24361 ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat atagaggttt
    24421 taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc
    24481 agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc
    24541 acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct gaactgaaca
    24601 agattgtttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct gcaagcgaag
    24661 cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc tggctgggcg
    24721 gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa gacctggaaa
    24781 ctcagtctca ggacggtact ttcgacaagc tgaccaagaa agaagcgctg atgcgcactc
    24841 gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc ggtctgccgg
    24901 acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa gcaaacaact
    24961 tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt gttgacttcg
    25021 ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg
    25081 caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa agcttcgtag
    25141 aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag aaaggcggca
    25201 agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga gcgaagacaa
    25261 cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta gtacacgttt
    25321 ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg tttaatgccg
    25381 ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc tggtaaaaga
    25441 gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga ctgaagctaa
    25501 cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa
    25561 aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg
    25621 catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg gtttccaggc
    25681 gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg ttgaagttct
    25741 gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa
    25801 cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc acggtgcgcg
    25861 tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac acatcgctat
    25921 gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg ctgaagtggt
    25981 agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc cgaaagaaat
    26041 cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg
    26101 tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag agcataacgc
    26161 tgaagtgact ggcttcatcc gtttcgaagt gggtgaaggc atcgagaaag ttgagactga
    26221 ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcaaaaa ggagccgcct
    26281 gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg gggctgcgct
    26341 gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca gtctcaggaa
    26401 agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa gttgagtggc
    26461 gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga tcgtatggct
    26521 caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat tggtgggggt
    26581 aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt gggcgaccac
    26641 atggggatgc tggcgaccgt aatgaacggc ttggcaatgc gtgatgcact gcaccgcgcc
    26701 tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga cagctacagc
    26761 tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca
    26821 ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga aattgaagcc
    26881 gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ccgctgatcc ggcgaaagat
    26941 ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa agagctgaaa
    27001 gtcatggacc tggcggcctt cacgctggct cgtgaccata aattaccgat tcgtgttttc
    27061 aatatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta
    27121 atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa gtatcgcggg
    27181 ggcgtaagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg ccagcgcgca
    27241 gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgctcggggc caaaatggca
    27301 aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag ggctatactt
    27361 agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc aaggattcgt
    27421 aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg cgtagaagcg
    27481 ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct gctggatggc
    27541 attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag cgtaacggta
    27601 gaagattccc gtactctgaa aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa
    27661 aaagcgatta tggcctccga tctcggcctg aacccgaact ctgcgggtag cgacatccgt
    27721 gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt
    27781 gaagcagaac aagcgcgtgt tgcagtacgt aatgtgcgtc gtgacgcgaa cgacaaagtg
    27841 aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc tcaggacgat
    27901 gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc agacaaagaa
    27961 gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt agatccttgc
    28021 ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt tcatgaagca
    28081 actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg tggtgcgcca
    28141 taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt
    28201 agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa
    28261 acttcttaaa acgatgctac agcaacaggg gagccgcacc gaagtcttaa gtgggcaaca
    28321 agccgcttgc gatatggcag cgcttgagga ggttgatcag gtgatggcag ccattgttgg
    28381 tgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc
    28441 caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa agcagagcaa
    28501 agcgcaattg ttaccggtcg atagcgaaca taacgctatt tttcagagtt taccgcaacc
    28561 tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt ccattttact
    28621 taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa caatgacgcc
    28681 cgatcaagct tgccgtcatc cgaactggtc gatgggacgt aaaatttctg tcgattcggc
    28741 taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta acgccagcgc
    28801 cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg tgcgctatca
    28861 ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa ttgcccacac
    28921 catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt gcaaactaag
    28981 tgcgttgaca tttgccgcac cggattatga gcgttatcca tgcctgaaac tggcgatgga
    29041 ggcgttcgaa caaggccagg cagcgacgac agcattgaat gccgcaaacg aaatcaccgt
    29101 tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga atttatccgt
    29161 actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc
    29221 gaacgcgagt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag gataatccgg
    29281 ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct gcgccatctg
    29341 atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg cttttttttg
    29401 tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa acgcgtgatg
    29461 ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg tcatgttgcg
    29521 atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg tgcctttggg
    29581 cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa caacggtatt
    29641 gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc gcaggaagtc
    29701 agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag tctgcaccga
    29761 cataacgtgc gtctgcgtat tattggcgat accagtcgtt ttaactcgcg tttgcaagaa
    29821 cgtattcgta aatctgaagc gctaacagcc ggaaataccg gtctgacgct gaatattgcg
    29881 gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc tgaaaaggtg
    29941 cagcaaggaa acctgcaacc agatcagata gatgaagaga tgttaaacca gcatgtctgt
    30001 atgcatgaac tggcccctgt agatttagta attaggactg ggggagagca tcgcattagt
    30061 aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt tctctggccc
    30121 gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt
    30181 ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat
    30241 cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg
    30301 ccggtggggt tcgccattgt aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga
    30361 cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta
    30421 ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca tcaaccgctg
    30481 gttgaaatct cactttgggc ttcgttgggt tggtggattg tcgcgctatt gctggtgctg
    30541 ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct tatttttggc
    30601 gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg gcactatgac
    30661 gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt atggggcgct
    30721 gactccggcg catatatgtt tggcaaattg tttggtaaac ataagctggc accgaaggtt
    30781 tctccgggta aaacctggca aggctttata ggtggactcg ctactgcagc ggtaatctca
    30841 tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact catttgctct
    30901 attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt taagcgtgaa
    30961 gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt agatcgtatt
    31021 gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt attcaggacg
    31081 ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc atcgttgcac
    31141 tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg cgttgtggtg
    31201 ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga actgataagc
    31261 tcggcaccga atatgttatc gccctgatcc cgttgggcgg ttatgtcaaa atgctggatg
    31321 agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat aaatctgtcg
    31381 gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt gctatctttg
    31441 cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt ggcgaaatag
    31501 cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta aaagccgtag
    31561 atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat aaaattggcg
    31621 atgaaagcac caccattaca gtagcgccat ttgacagcga ccaacggcgg gatgtaaagc
    31681 tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct tcgctgggga
    31741 ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcagcca aactcggcgg
    31801 caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa
    31861 cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc ttagcgttag
    31921 aaatcgaaag gcaggggagt cccttgtctt tgacattaat cccggagagt aaaccgggta
    31981 atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg ccagatgagt
    32041 ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg gacaaaacgt
    32101 ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac
    32161 tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca gcggaactcg
    32221 gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg ataattaacc
    32281 tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc gaaaagatca
    32341 agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc tcgattctgc
    32401 tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga gagttagtta
    32461 ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag
    32521 cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct
    32581 tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgtacag gcgacacggt
    32641 taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca actttgagga
    32701 tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac gtccgaccat
    32761 tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga agcaaaacct
    32821 cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg ccgatatcga
    32881 gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg ttaaagctgt
    32941 cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg aaggtgtgtc
    33001 agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg acgaactgat
    33061 ctctcatttc caactgcgtg acgaagtgcc gtggtggaat gtggtaggcg atcgtaaata
    33121 ccagaaacag aaactggcgg gcgatcttga aaccctgcgc agctactatc tggaccgcgg
    33181 ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgccagata aaaaaggtat
    33241 ttacgtcacg gtgaacatca ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag
    33301 cggcaacctt gccgggcact ccgctgaaat tgagcaactg actaagatcg agccgggcga
    33361 gctgtataac ggcactaaag tgaccaaaat ggaagatgac attaaaaagc ttctcggtcg
    33421 ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac
    33481 cgttaaatta cgcgtgaacg ttgatgcggg taaccgtttc tacgtgcgta agatccgttt
    33541 tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg
    33601 tgcatggttg gggagcgatc tggtcgatca gggtaaggag cgtctgaatc gtctgggctt
    33661 ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggacc aggttgatgt
    33721 agtctacaag gtgaaagaac gtaacactgg tagcttcaac ttcgggattg gttacggtac
    33781 tgaaagtggc gtgagcttcc aggctggcgt gcaacaggat aactggttag gtacaggtta
    33841 tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac
    33901 caacccgtac ttcaccgtag atggcgtaag cctcggtggt cgtctcttct ataatgactt
    33961 ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta cagacgtgac
    34021 gttgggcttc ccgattaacg aatataactc gctgcgtgca ggtctgggtt atgtacataa
    34081 ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact ctatgggtga
    34141 acatccgagc acctctgatc aggataacag cttcaaaacg gacgacttca cgttcaacta
    34201 tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa
    34261 cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag tgacgttaga
    34321 cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg gtcgtacccg
    34381 ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga acttctatgc
    34441 cggtggttcc agcaccgtgc gtggcttcca gtccaatacc attggtccga aagcagttta
    34501 cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg cgactcagga
    34561 cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca tggcggttgc
    34621 cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta actcggttcg
    34681 tacttcattc ttctgggata tgggtaccgt ttgggataca aactgggatt ccagccaata
    34741 ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg gtatcgcatt
    34801 acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt tcaaaaagta
    34861 cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt aagtgttctc
    34921 cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata agatcgccgg
    34981 gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt attgtgaaaa
    35041 agtggttatt agctgcaggt cttggtttag cactggcaac ttctgctcag gcggctgaca
    35101 aaattgcaat cgtcaatatg ggcagcctgt tccagcaggt agcgcagaaa accggtgttt
    35161 ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg
    35221 atctgcaggc taaaatgaaa aaactgcagt ccatgaaagc gggcagcgat cgcaccaaac
    35281 tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag gcttttgagc
    35341 aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt atccagactg
    35401 ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca aacgccgttg
    35461 cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag gttaaataag
    35521 taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa ctacacggtg
    35581 atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta
    35641 cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg tccgcggttg
    35701 tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg aagaatccct
    35761 acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc gcgcagaaca
    35821 ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaacgta tcgattggcg
    35881 ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt
    35941 gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg aacgtaacca
    36001 tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg
    36061 cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca cagattggtc
    36121 gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat cgcggcgcgc
    36181 tggatgacac tgttattggc aatggcgtga tcattgataa ccagtgccag attgcacata
    36241 acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg ggcagcctga
    36301 aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat atggaaatat
    36361 gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact gaaccaggcg
    36421 tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg
    36481 tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac
    36541 aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt cttacgattg
    36601 cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta tcttgactac
    36661 taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc gtttcccgtt
    36721 cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa
    36781 tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga ttttcccggg
    36841 tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta aaagcgtagg
    36901 aaaactggaa ccgggtgagc tgtactactt cgctggtatt gacgaagcgc gcttcaagcg
    36961 cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg
    37021 cctgacccgt tttaaagggg ttgctctggt cgatggtaaa gtagtttgcg aagcaacgat
    37081 gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct ttgtgcatcc
    37141 aaccgccatt gtggaagagg gcgcgtcaat tggcgcgaac gcacacattg gtcctttttg
    37201 tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc acgttgtcgt
    37261 gaatggtcat actaaaattg gccgcgataa tgagatttat cagtttgcct ccatcggcga
    37321 agttaaccag gatttgaaat atgctggcga accgacccgt gtggaaatcg gcgatcgtaa
    37381 ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg gattgacgaa
    37441 ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg
    37501 taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg ttgacgactt
    37561 cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg cgcatgtgat
    37621 ggttggcggc tgctccggtg tggcgcagga cgtccctcct tatgttattg cgcagggtaa
    37681 ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat tcagccgtga
    37741 ggcgattacc gctatccgca atgcgtataa gctaatttat cgtagcggta aaacgctcga
    37801 tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat ccggaagtga aagcctttac
    37861 cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgacca gcgtccatta
    37921 acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg tttaatccgc
    37981 gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg ttgccgggcc acgaatgcag
    38041 gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg cattgttgaa
    38101 gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaaa gcgttttggc
    38161 gaactgaagc cagatgtttt tgttggtatt gatgcgcccg acttcaatat tactcttgaa
    38221 ggtaacctca aaaagcaggg tatcaaaacc attcattacg tcagtccatc cgtctgggcg
    38281 tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct cgcatttctg
    38341 cctttcgaaa aagcgtttta tgacaaatac aatgtaccgt gccgctttat cggtcatacc
    38401 atggctgatg ccatgccgtt agatccagat aaaaatggtg cccgtgatgt gctggggatc
    38461 ccttacgatg cccactgtct ggcattgttg ccgggcagcc gtggtgcaga agtcgaaatg
    38521 cttagtgccg atttcttgaa aactgcccag cttttgcgcc agacatatcc ggatctcgaa
    38581 atcgtggtgc cgctggtgaa tgccaaacgc cgcgagcagt ttgaacgcat caaagctgaa
    38641 gtcgcgccag acctgtcggt tcatttgctg gatggaatgg gccgtgaggc gatggtcgcc
    38701 agcgatgcgg cactactggc gtcggggacg gcagccctgg agtgtatgct ggcgaaatgc
    38761 ccgatggtgg tgggatatcg catgaagcct tttaccttct ggttggcgaa gcggctggtg
    38821 aaaactgatt atgtctcgct gccaaatctg ctggcgggca gagagttagt caaagagtta
    38881 ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct gttggcgaac
    38941 gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca gatccgctgc
    39001 aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc gaatttgttt
    39061 atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg
    39121 cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc gattgccggg ctgaatgatt
    39181 ccaaaaagct gagcgaaaaa cgccgtctgg cgctctatga agagatcaaa gagaaagcgt
    39241 tgagctggag tctgggccgc gcggaacccc acgaaatcga cgagctgaac attcttcatg
    39301 cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca tattgcgccg gaatatgtgt
    39361 tgattgatgg taaccgctgc ccgaaattac cgatgcctgc gatggctgtg gtgaaagggg
    39421 atagccgcgt accagaaatc agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg
    39481 aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacat aaagggtatc
    39541 caaccgcttt tcatctggaa aaactggctg aatatggcgc gactgaacat catcgtcgca
    39601 gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt cgagattaag
    39661 taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg cacagcgact
    39721 actcgatgat cgatggcttg gccaaaaccg caccgctggt aaaaaaggcg gcggcgttgg
    39781 gtatgccagc actggcgatc accgatttca ccaacctttg cggtctggtg aagttctacg
    39841 gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaat gtccagtgcg
    39901 acctgctggg tgatgagtta acccacctga cggtactggc ggcgaacaat accggctatc
    39961 agaatctggc gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga
    40021 tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt tccggcgggc
    40081 gcatgggcga cgtcggacgc agtcttttgc gtggtaacag cgcgctggta gatgagtgtg
    40141 tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc cgcaccggca
    40201 ggccggatga agaaagctat ctgcacgcgg cggtggaact ggcggaagcg cgcggtttgc
    40261 ccgtcgtggc gactaacgat gtgcgcttta tcgacagcag cgactttgac gcacacgaaa
    40321 tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg cgtaactatt
    40381 cgccgcagca atatatgcgt agcgaagagg agatgtgtga gctgtttgcc gacatcccag
    40441 aagcccttgc caacaccgtt gagatcgcca aacgctgtaa cgtaactgtg cgtcttggtg
    40501 aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat ctggtcaagc
    40561 gtgcaaaaga aggcctggaa gagcgtctgg catttttatt ccctgacgag gaagaacgtc
    40621 ttaaacgccg cccggaatat gacgaacgtc tggagactga acttcaggtt atcaaccaga
    40681 tgggcttccc gggctacttc ctcatcgtta tggaatttat ccagtggtcg aaagataacg
    40741 gcgtaccggt agggccaggc cgtggctccg gtgcgggttc actggtggcc tacgcgctga
    40801 aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc cttaacccgg
    40861 aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc gatcaggtta
    40921 tcgaacacgt ggcggacatg tacggtcgtg atgcggtatc gcagattatc accttcggta
    40981 cgatggcggc gaaagcggtg atccgcgacg taggccgcgt gctggggcat ccgtacggct
    41041 ttgtcgaccg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg gcgaaagcgt
    41101 ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt aaggcgctga
    41161 tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac gccggtgggg
    41221 tggttatcgc gccgaccaaa attaccgatt ttgcgccgct ttattgcgat gaagagggca
    41281 aacatccggt cacccagttt gataaaagcg acgttgaata cgccggactg gtgaagttcg
    41341 acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg atcaacaagc
    41401 ggcgggcgaa gaatggcgag ccaccgctgg atatcgccgc gatcccgctg gatgacaaga
    41461 aaagcttcga catgctgcaa cgctcggaaa ccacggcggt attccagctt gaatcgcgcg
    41521 gcatgaagga cctgatcaag cgtctgcaac ctgactgctt cgaagatatg atcgcactgg
    41581 tggcactgtt ccgccctggt ccgttgcagt cagggatggt ggataacttt atcgaccgta
    41641 aacatggtcg tgaagagatc tcctatccgg acgtgcagtg gcagcatgaa agcctgaaat
    41701 cggtactgga gccaacctac ggcattatct tgtatcagga acaggtcatg cagattgccc
    41761 aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgccgtgcg atgggtaaga
    41821 aaaagccgga agagatggct aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg
    41881 gaatcaacgc cgaactggcg atgaaaatct tcgacctggt ggagaaattc gcgggttacg
    41941 gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg ttatggctga
    42001 aagcgcacta tccggcggag tttatggcgg cggtaatgac cgccgatatg gacaacaccg
    42061 agaaggtggt gggcctggtg gatgagtgct ggcggatggg gctgaaaatc ctgccaccag
    42121 atataaactc cggtctttac catttccacg tcaacgacga tggcgaaatc gtgtatggta
    42181 ttggcgccat caaaggggta ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata
    42241 aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa
    42301 accggcgggt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc
    42361 gcgcggcgct gatgaactcg ctgggtgatg cgttaaaagc ggcagatcaa cacgcgaaag
    42421 cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg gaacaaattg
    42481 aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat ggggaacgtg
    42541 aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa gagattgagc
    42601 gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt aaagtcatca
    42661 cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg
    42721 gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt actgacgccc
    42781 tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc ggacaggtca
    42841 gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg
    42901 aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg caaattgatg
    42961 accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg acaattccag
    43021 tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc
    43081 gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt tcggagcagg
    43141 tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt gattttgaac
    43201 agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc cgtcaggatg
    43261 agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac
    43321 tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg gcacgccatc
    43381 cgcagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt gacgaactgg
    43441 ctggcgaccg tgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg
    43501 gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc
    43561 gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg
    43621 ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct tatcctggcg
    43681 tgggcgcaga agagcgtggt cagtctgaag ccattgcacg caacctgcgt gaaatgtctc
    43741 gccttggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggc ggtgcgctgg
    43801 cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc gttatctcgc
    43861 cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agcgccgctg gcggctgaag
    43921 cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc atcatcccgg
    43981 aaccgctggg cggcgctcac cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac
    44041 tgctggcgga tctggccgat ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt
    44101 atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa aagggtcact
    44161 tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa ggattttcca
    44221 ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt ataaagatga
    44281 gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta tctggccaca
    44341 aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg gcgtgatttt
    44401 tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta atgaatatct
    44461 cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc aggatatgcg
    44521 gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg ccattcgtat
    44581 gcgtcagtac accaacgaat atcttgataa cattacgccg ccgttcacga aagccttgtt
    44641 tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg gcggcaccgc
    44701 atatcaaaaa agcccggttg gctgtctgtt ttatgatttt ttcggcggga atacccttaa
    44761 ggctgatgtc tctatttcgg tcaccgagct tggttcgttg cttgaccaca ccgggccaca
    44821 cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt atatcgttac
    44881 caacggaata tcgacgtcga acaaaattgt gggtatgtac gccgcgccat ccggcagtac
    44941 gctgttgatc gaccgcaact gccataaatc actggcgcat ctgttgatga tgaacgatgt
    45001 agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attctggggg ggatcccgcg
    45061 ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccactacgc aagcacaatg
    45121 gccggttcat gcggtgatca ccaactccac ctatgatggt ttgctctaca acaccgactg
    45181 gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg tgccgtatac
    45241 tcattttcat ccaatctacc agggtaaaag tggtatgagc ggcgagcgtg ttgcgggaaa
    45301 agttttcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc aggcttcact
    45361 gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta tgatgcatac
    45421 caccacctcg cccagttatc ccattgttgc ttcggttaag acggcggcgg cgatgctgcg
    45481 tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc attttcgcaa
    45541 agaggtccag cggctgcggg aagagtctga cggttggttt ttcgatatct ggcaaccgcc
    45601 gcaggtggat gaagccgaat gcaggcccgt tgcgcctggc gaacaatggc acggctttaa
    45661 cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt tgacaccggg
    45721 gatggacgaa cagggcaata tgagtgagga ggggatcccg gcggcgctgg tagcaaaatt
    45781 cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc tgtttctctt
    45841 tagtattggc atcgataaaa ccaaagcaat gggattattg cgtgggttga cggaatttaa
    45901 gcgctcttac gatctcaacc tgcggatcaa aaatatgctg cccgatctct atgctgaaga
    45961 tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc ataagctgat
    46021 tcgtaaacac gatctttccg gtttgatgtt gcgggcattc gatactttgc cggaaatgat
    46081 catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa ccattgcgct
    46141 ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac cgggcgtacc
    46201 gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg attttctact
    46261 gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc acggcgcgaa
    46321 acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat aacttgccag
    46381 agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat gctgggttta
    46441 aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa agctttctac
    46501 tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg
    46561 aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt tccgttcccg
    46621 ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat
    46681 gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga agccatccgt
    46741 gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg gctgccgttg
    46801 gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt
    46861 gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc tcacctcacg
    46921 ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc atcagttggt
    46981 gcagtggcgg acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc atcacggttt
    47041 aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac agtggcaggt
    47101 gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg ctggggattg aggcccaggc
    47161 gcggcaggca cgttatcagg catttgcccg taccctattg cccggcgaag tgctggtcac
    47221 cgcgcaacat ctcgacgatc aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg
    47281 ccctgccggg ctttcggcta tggcggaagt ttcggagttt gctggaacac ggcttatccg
    47341 cccgttgctt gcccgcacgc ggggggaact ggagcagtgg gcattggcgc atggtttacg
    47401 ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc tgcgcctgcg
    47461 cgtagtgccg ttattgcagc agcgttggcc gcattttgcg gaagcaacgg cccgcagcgc
    47521 cacactttgt gctgaacaag agagtctgtt ggatgaactg ctggcggatg atttagcaca
    47581 ctgccaaacg tcgcagggga cgctgcagat tgcgccaatg ctggcgatga gtgatgcccg
    47641 tcgcgcggcg attattcgcc gctggctggc agggcagaat gcaccgatgc cttcccgcga
    47701 cgcgctggtg cgcatctggc aggaagtggc gctggcgcgg gaagatgctt ccccctgttt
    47761 acgtttgggc gcctttgaaa tccgccgcta tcagtcgcaa ctgtggtgga ttaaatccgt
    47821 caccgggcaa agcgaaacca ttgtgctgtg gcagacgtgg ctacaaccgc tggaattacc
    47881 ggcggggctg ggaaccgtac agcttactgc gggaggcgat attcgccctc cgcgtgcaga
    47941 cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg catattgtcg ggcgcaacgg
    48001 cggacgtaag ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc tacgtgacac
    48061 cacgccactg ctgttttatg gagaaacgct gattgcggcg gcaggggtat ttgtgacgca
    48121 agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa cgcttagtta
    48181 agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat caggattcgc
    48241 ttaccaccac cgtaccgatt tcagggtggc taaagctggt aattttatcc agacgcagct
    48301 cgcgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg gagactaaat
    48361 cactggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc attaaatgat
    48421 gctggcaggc gagctcaaga ttatcgtaat catcacaatt aattggttga tacgtatcat
    48481 tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt ttccctgaca
    48541 gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac gcagcccagc
    48601 gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt gcgtattaac
    48661 tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg gatgcaagtc
    48721 taagcctacg aagataaact ctgtttcgca aggtgactat accacactca tttctgcaat
    48781 atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa aggagttttc
    48841 ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat ctcgaccgca
    48901 accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta
    48961 tgatgctgcg cttgctggcg tggctgaaat atgctgatga acgtctgcaa tttacccgtg
    49021 gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt
    49081 tgtggattga gctggggctg ccggatgagc gacgaattaa gaaagcctgc acccaggccg
    49141 cagaagtggc gctgtttacc tataatagtc gggcggcgca aatctggtgg cagcaaaatc
    49201 agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat gaacaactgg
    49261 cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt caggatggtg
    49321 ttatctggtt atcggatgat aagaataatc tggaagttaa cttaaccgcc tggcaacaac
    49381 cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg agatcaccgc
    49441 cattcgtgcg cagggcgctg gcgggcagca tgttaataag acctcaacgg ctattcatct
    49501 gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc tgctcgccgc
    49561 cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg aataccgcag
    49621 tcaggaactg aaccgcgaag ccgctctggc ccggctggtg gcagtgatta aagatttaac
    49681 aaaagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag agcgcaggct
    49741 ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtac gcagcggtcg
    49801 ggaataaaaa gaaggaatgg atggtgaaaa aagcgatagt gacagcgatg gctgtaatca
    49861 gcctctttac tctgatggga tgtaataatc gggccgaagt cgatacgctt tctccggcgc
    49921 aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctaccg tgtgccgatt
    49981 gcgaaggaat cgaaatctct ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc
    50041 gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa
    50101 ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat cgggcgaaag
    50161 gcgatgcgct ggagatgctc gatcgtgaag gcaatccaat tgaatcgcag ttcaactata
    50221 cgctggaacc ggcacaatcc agtttaccta tgacgccgat gaccctgcga ggcatgtatt
    50281 tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt ttcatggtag
    50341 cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt gaaaaaccgg
    50401 tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact ggtgcgccaa
    50461 ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat tgcagtagtt
    50521 tggggcagta aacctgctta aggcagaaac aaacgcaaaa ttgcctgatg cgctgcgctt
    50581 atcaggccta cagcatataa tgcaacattt tgaattagca cgattttgta ggccggataa
    50641 ggcgtttacg ccgcatccgg cattttgtgc acgaggctgg ctttatcact gagtcagccc
    50701 aacaaagcga cccgccttga tataaatccc aggcttcagg tgcccaacat actgtaactg
    50761 cgtttctggc ttaaacgcag agacatcacc aatacgcata tgcatcagat cggcagggct
    50821 aatccagccc gattgcgcca gtgtaagtgg atgcccggat ttggcaaatg catcagtgac
    50881 aaattccgaa caaaaccacg actttttgtc tccttcgcac acactgctta actgcgcttt
    50941 cgccaggtcg ctgacgcact gttggcgaaa atcctcggag aacggattca gtgagcacat
    51001 ctggcgagtc accataaagg gaataaattc gacaatgccg cgatagttat aaccgctatc
    51061 tttgatttta ttggcaaacg cggtgatatc tgtggcttgt tgcggggtaa gatccgggac
    51121 tcgtaagacg aaaagcttat cactatgctt catcgctttt ttaagggaaa caatctggac
    51181 gccagcgcct gtcgcttctg caacgttatt atcacccaga aagattgcaa cgtgactcac
    51241 agaggaagtg ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg aggagaacag
    51301 caaatcgccg ggtttgagat caggtgccgt tatttctttt attgattgtt cggtgaaaga
    51361 gctttgatgc tggaatttga cagcccatgt ttttgcctcg gcatctactg ctgtggctga
    51421 tgggtctggc tggctgatat caacggtaca agccgaaagt aaaagaaagc tggggagaag
    51481 caggcggcag tacgcctttg gtttatccat tttatacaat ccatgtaaaa aagggccctg
    51541 aaattcagga ccctttctgg catcagcctt taatctgttt caccagatat tcgacgatgt
    51601 caccagtctt aattaactgt ttctcgccgt tgcgacgata tttatattcg atatcgtcgt
    51661 tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt tccatatcag
    51721 caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata ccttgtgcac
    51781 gcagttcgct gtacagtttc tcagcaagct cttgtacgcg gaaggatttg tgcatgttca
    51841 tcggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg ccgcgttcgt
    51901 cgtagttctg ctcaatcgcc gcagctacca cacgcgttac cccgataccg tagcaaccca
    51961 tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt gcttcggagt
    52021 acttggtacc cagctggaag atgtgaccaa cttcgatacc acgtttgatc agcagcgtac
    52081 cctggccatc cgggcttgga tcgccagcca ccacgttacg gatatctgca acttccgggg
    52141 tagcgacatc gcgatcccag ttgataccga agtagtgttt accatcgatg ttagcaccag
    52201 cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc ggcatgttta
    52261 ccggacccag tgaacccgga ccggctttaa ccacagcacg aatttcttct tcggtcgcga
    52321 aagtcagcgg gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc agctcgtgat
    52381 caccgcgcac cagcagcgca accagcggga agctactgcc ttcaaccgct ttaaccagca
    52441 gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc gcgatggttt
    52501 tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc ggttctttcg
    52561 gcgcgatagc ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg gagaagacca
    52621 catcgtcttc accgctctgc gccagcacct ggaattcgtg agaggcgctg ccgccgatag
    52681 aaccggtgtc ggcctgtacg gcgcggaaat ccagccccat gcggctgaag attttgctgt
    52741 aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta tggaaagagt
    52801 aagcatcttt catcaggaat tcgcgggaac gcatgacgcc gaaacgcgga cgcacttcgt
    52861 cgcggaactt ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa gagctaagct
    52921 cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg aacggacgct
    52981 cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga ccactctctt
    53041 gccacaaatc ggctggctga accaccggca tcgacacctc gatcgcaccg gcgttgttca
    53101 tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc ggcagccagg
    53161 tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc tgatggctga
    53221 tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg ctagtacgca
    53281 tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa
    53341 gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg
    53401 ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg
    53461 tagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc
    53521 ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct ccagcgtcaa
    53581 aagctgcttt tcgacctctg cggtaaaact caccgccatc tctgcagctg gcgcactttg
    53641 cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg gtttgatatc
    53701 cactaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca cgctgccttt
    53761 atggcaaaca acctctttca gctctaccag cgacatgcca attgggttag ggcggaaagt
    53821 agagcgtgtt gcgaaaaccc ccattctggc gttaccgccg aggcgcggtg gacgcacagt
    53881 cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata aatggctgaa
    53941 tgcttccagg ccgcgaacgg cgtcggcctg gttgtaggaa gctatgagat gcagttcgcc
    54001 gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt tatagggcga
    54061 gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat gttaagcgca
    54121 gagccgatac atacagcctg acgatagcag cccggcgtac cgctggtgac ttcgcagcta
    54181 tgcagtaata cagcattggc tttcatttta gaggcattga tttgcatctg cttacgtgcg
    54241 gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc actgacttca
    54301 ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat tcggaccggc
    54361 gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc agtgctttga
    54421 acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag tgctactaaa
    54481 cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc aatattgaat
    54541 caggagcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata ttacaaattg
    54601 tggaaacagc ctaaaaatta ccagccttta acagcgccgc cgttaaacac tttgtttgct
    54661 gcttcgtaaa cttcatcaga ctgataagcc tggacgaatt tcttcacgtt ctcggcgtct
    54721 ttgttatctt cacgcgttac gatcaggttt acgtatgggg agtctttatc ttcgacaaag
    54781 ataccgtctt tcgccggagt caggccaatc tggctggcat aggtggtatt gataactgcc
    54841 agagcgattt gcgcgtcgtc cagagaacgc ggcagttgcg gtgcttccag ttcaacaatt
    54901 ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc aacgccatct
    54961 ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag attagttgga
    55021 tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt gattttcttg
    55081 gagtaaccag caatcggata aacaaaagtg ttgcctactg cgaccagttt gtagccacga
    55141 tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc gatatcgcct
    55201 ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc aacgtccagg
    55261 ccatatttgt ctttcgcaac tttctgcgcg acttctgcta cctgctgttc ggcaccaaca
    55321 atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc taccagtgcc
    55381 agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc cataccttat
    55441 tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg
    55501 cggtcgcctg caaactgaat taaataaacc agaatgacca gcaataccag taccgtattc
    55561 atcaccgtcg cgttgtagcc gatgtagcca tactgatagc caatctgacc taaaccaccg
    55621 gcaccgactg caccacccat cgcggaataa ccgaccaggg taatcagggt gatagttgcc
    55681 gcattcacca gacccggcaa cgcttccggt aacagcactt tacagacgat ctgcattggc
    55741 gtggcaccca ttgcgcggga agcttcaatt aacccggttg ggatctccag cagagcgttc
    55801 tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat tgctgcctgc
    55861 aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac cagcaagata
    55921 atgaacggga tggaacggaa aatgttcaca atcgcagaaa cggtacgata cagcttcgcg
    55981 ttagcaataa tttgccccgg acgcgtgaca taaagcagaa cgccaaccgg cagaccaatc
    56041 acaaacccaa aaaatccgga tacgaaggtc attgccagcg tttcccatac gccacgaacc
    56101 agcagccaca tcatcggctc agacataacc cagtacctct acttttacat ggtgttcctg
    56161 cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag tcagcatgat
    56221 gccgaattta acgccaccgg cgtagtccat ctgcgcgcta ataatgttgt tgttgacgtt
    56281 gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg taaactccag
    56341 acgcagcatc ggcacgcagt cagtaaatgg ctccgtttgc agacgttcct ggtaatcttc
    56401 cgggatatcc agatgcaggg tcgactgaat aaacttctgc gccagcggcg ttttcggatg
    56461 cgagaacact tcacttaccg tgtcctgctc gatcagttcg ccattgctga tgaccgccac
    56521 gcaatcacaa atacgcttca caacgtccat ttcgtgggtg atcaacagaa tcgtcaaacc
    56581 cagacggcgg ttaatgtctt tcagcagttc gagaatagaa cgtgtcgttg ccgggtccag
    56641 cgcgctggtg gcttcatcac acagcaatac tttgggattg ctggctaatg cacgggcaat
    56701 cgccacacgt tgtttctgcc caccggaaag attcgacggg tagctatcat gcttatcgcc
    56761 aagaccaacc aatgacagca attccgtcac gcgacgtttg atctcgtctt tcggtgtgtt
    56821 gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa
    56881 atgctggaaa atcataccaa tctggcggcg agctttggtc aactcggatt ctgacagcgt
    56941 ggtcaattcc tggccatcga ccagcacgct accctcggtt gggcgctcca gcaagtttac
    57001 acaacgtata agcgtactct tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc
    57061 agctggcaca tgcaggctga cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa
    57121 cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat taagttagtc
    57181 gtagcatctc gaatgcctga aacgggcaac gccgtcaatg aaatggatgt taaggcatcc
    57241 agacgtctaa atcaatcagg tttatgcgaa gagcactttc ttgcaggtcg aaacatgcga
    57301 tactagcgtc ccatgcctta ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat
    57361 ttttcttgac cgtgatggca ccattaatgt cgatcacggc tatgtccatg agatcgacaa
    57421 ctttgaattt atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc
    57481 gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttaccg aagcacagtt
    57541 tgaaacgctg accgagtgga tggactggtc gctggcggac cgagatgtcg atctggatgg
    57601 tatctattat tgcccgcatc atccgcaggg gagtgttgaa gagtttcgcc aggtctgcga
    57661 ttgccgcaaa ccacatccgg ggatgctttt gtcagcacgc gattatttgc atattgatat
    57721 ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg ttgcggcgaa
    57781 cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt acacctgaag cagaaaacgc
    57841 ggcagattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa
    57901 accggcgcaa tgattaaaag atgaacggtt gaaataaaaa tgcatttttc cgcttgtctt
    57961 cctgagccga ctccctataa tgcgcctcca tcgacacggc ggatgtgaat cacttcacac
    58021 aaacagccgg ttcggttgaa gagaaaaaat cctgaaattc agggttgact ctgaaatagg
    58081 aaagtgtgat atacgccacc tcgcgacagt gagctgaaag ctgcgtcgca actgctcttt
    58141 aacaatttat cagacaatct gtgtgggcac tcgaagatac ggattcttaa cgtcgcaaga
    58201 cgataaatga ataccaagtc tcaagagtga acacgtaatt cattacgaag tttaattctt
    58261 tgagcatcaa acttttaaat tgaagagttt gatcatggct cagattgaac gctggcggca
    58321 ggcctaacac atgcaagtcg aacggtaaca ggaagcgctt gctgcttcgc tgacgagtgg
    58381 cggacgggtg agtaatgtct gggaaactgc ctgatggagg gggataacta ctggaaacgg
    58441 tagctaatac cgcataacgt cgcaagacca aagaggggga ccttcgggcc tcttgccatc
    58501 ggatgtgccc agatgggatt agcttgttgg tggggtaacg gctcacc
//
LOCUS       DAZPEU010000004        55548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPEU010000004 DAZPEU010000000
VERSION     DAZPEU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.004"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            147..1633
                     /locus_tag="ACHU2L_000164"
                     /pseudo
     CDS             147..1633
                     /locus_tag="ACHU2L_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            1648..2382
                     /gene="ypdB"
                     /locus_tag="ACHU2L_000165"
     CDS             1648..2382
                     /gene="ypdB"
                     /locus_tag="ACHU2L_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR1355202.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2395..3252
                     /locus_tag="ACHU2L_000166"
                     /pseudo
     CDS             2395..3252
                     /locus_tag="ACHU2L_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3255..5757)
                     /gene="ptsP"
                     /locus_tag="ACHU2L_000167"
                     /pseudo
     CDS             complement(3255..5757)
                     /gene="ptsP"
                     /locus_tag="ACHU2L_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5782..6826)
                     /gene="ypdE"
                     /locus_tag="ACHU2L_000168"
                     /pseudo
     CDS             complement(5782..6826)
                     /gene="ypdE"
                     /locus_tag="ACHU2L_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(6826..7242)
                     /locus_tag="ACHU2L_000169"
     CDS             complement(6826..7242)
                     /locus_tag="ACHU2L_000169"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR1355203.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(7239..7346)
                     /locus_tag="ACHU2L_000170"
     CDS             complement(7239..7346)
                     /locus_tag="ACHU2L_000170"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355204.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(7307..7909)
                     /gene="ypdF"
                     /locus_tag="ACHU2L_000171"
                     /pseudo
     CDS             complement(7307..7909)
                     /gene="ypdF"
                     /locus_tag="ACHU2L_000171"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7924..9171)
                     /locus_tag="ACHU2L_000172"
     CDS             complement(7924..9171)
                     /locus_tag="ACHU2L_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR1355205.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9193..9519)
                     /locus_tag="ACHU2L_000173"
     CDS             complement(9193..9519)
                     /locus_tag="ACHU2L_000173"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR1355206.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9738..10703)
                     /gene="glk"
                     /locus_tag="ACHU2L_000174"
     CDS             complement(9738..10703)
                     /gene="glk"
                     /locus_tag="ACHU2L_000174"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR1355207.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            10907..12163
                     /locus_tag="ACHU2L_000175"
     CDS             10907..12163
                     /locus_tag="ACHU2L_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR1355208.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12278..12604
                     /gene="ypeC"
                     /locus_tag="ACHU2L_000176"
     CDS             12278..12604
                     /gene="ypeC"
                     /locus_tag="ACHU2L_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR1355209.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12745..13983)
                     /locus_tag="ACHU2L_000177"
     CDS             complement(12745..13983)
                     /locus_tag="ACHU2L_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR1355210.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14319..15521
                     /gene="nupC"
                     /locus_tag="ACHU2L_000178"
     CDS             14319..15521
                     /gene="nupC"
                     /locus_tag="ACHU2L_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR1355211.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15571..17683)
                     /locus_tag="ACHU2L_000179"
                     /pseudo
     CDS             complement(15571..17683)
                     /locus_tag="ACHU2L_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(17891..17966)
                     /locus_tag="ACHU2L_000180"
     tRNA            complement(17891..17966)
                     /locus_tag="ACHU2L_000180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17931..17933),aa:Ala,seq:ggc)
     gene            complement(18006..18081)
                     /locus_tag="ACHU2L_000181"
     tRNA            complement(18006..18081)
                     /locus_tag="ACHU2L_000181"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18046..18048),aa:Ala,seq:ggc)
     gene            18302..18661
                     /locus_tag="ACHU2L_000182"
     CDS             18302..18661
                     /locus_tag="ACHU2L_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1355212.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18663..19055
                     /locus_tag="ACHU2L_000183"
     CDS             18663..19055
                     /locus_tag="ACHU2L_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1355213.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19107..20522)
                     /gene="gltX"
                     /locus_tag="ACHU2L_000184"
     CDS             complement(19107..20522)
                     /gene="gltX"
                     /locus_tag="ACHU2L_000184"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR1355214.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20781..20856
                     /locus_tag="ACHU2L_000185"
     tRNA            20781..20856
                     /locus_tag="ACHU2L_000185"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20814..20816,aa:Val,seq:tac)
     gene            20901..20976
                     /locus_tag="ACHU2L_000186"
     tRNA            20901..20976
                     /locus_tag="ACHU2L_000186"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20934..20936,aa:Val,seq:tac)
     gene            20981..21056
                     /locus_tag="ACHU2L_000187"
     tRNA            20981..21056
                     /locus_tag="ACHU2L_000187"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21014..21016,aa:Lys,seq:ttt)
     gene            21087..21212
                     /locus_tag="ACHU2L_000188"
     ncRNA           21087..21212
                     /ncRNA_class="other"
                     /locus_tag="ACHU2L_000188"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21332..21673
                     /locus_tag="ACHU2L_000189"
     CDS             21332..21673
                     /locus_tag="ACHU2L_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR1355215.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(21664..22590)
                     /locus_tag="ACHU2L_000190"
     CDS             complement(21664..22590)
                     /locus_tag="ACHU2L_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1355216.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            22680..23678
                     /locus_tag="ACHU2L_000191"
     CDS             22680..23678
                     /locus_tag="ACHU2L_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR1355217.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23675..23893)
                     /locus_tag="ACHU2L_000192"
     CDS             complement(23675..23893)
                     /locus_tag="ACHU2L_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR1355218.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23895..25910)
                     /gene="ligA"
                     /locus_tag="ACHU2L_000193"
     CDS             complement(23895..25910)
                     /gene="ligA"
                     /locus_tag="ACHU2L_000193"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR1355219.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25981..26967)
                     /gene="zipA"
                     /locus_tag="ACHU2L_000194"
     CDS             complement(25981..26967)
                     /gene="zipA"
                     /locus_tag="ACHU2L_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR1355220.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            27197..27958
                     /gene="cysZ"
                     /locus_tag="ACHU2L_000195"
     CDS             27197..27958
                     /gene="cysZ"
                     /locus_tag="ACHU2L_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR1355221.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            28143..29114
                     /gene="cysK"
                     /locus_tag="ACHU2L_000196"
     CDS             28143..29114
                     /gene="cysK"
                     /locus_tag="ACHU2L_000196"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR1355222.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29498..29755
                     /gene="ptsH"
                     /locus_tag="ACHU2L_000197"
     CDS             29498..29755
                     /gene="ptsH"
                     /locus_tag="ACHU2L_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR1355223.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29800..31527
                     /gene="ptsI"
                     /locus_tag="ACHU2L_000198"
     CDS             29800..31527
                     /gene="ptsI"
                     /locus_tag="ACHU2L_000198"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR1355224.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31568..32077
                     /gene="crr"
                     /locus_tag="ACHU2L_000199"
     CDS             31568..32077
                     /gene="crr"
                     /locus_tag="ACHU2L_000199"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR1355225.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32119..32970)
                     /gene="pdxK"
                     /locus_tag="ACHU2L_000200"
                     /gene_synonym="thiJ"
     CDS             complement(32119..32970)
                     /gene="pdxK"
                     /locus_tag="ACHU2L_000200"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR1355226.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            33075..33449
                     /locus_tag="ACHU2L_000201"
     CDS             33075..33449
                     /locus_tag="ACHU2L_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR1355227.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            33482..34205
                     /locus_tag="ACHU2L_000202"
                     /pseudo
     CDS             33482..34205
                     /locus_tag="ACHU2L_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(34409..35320)
                     /gene="cysM"
                     /locus_tag="ACHU2L_000203"
     CDS             complement(34409..35320)
                     /gene="cysM"
                     /locus_tag="ACHU2L_000203"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR1355228.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(35454..36551)
                     /gene="cysA"
                     /locus_tag="ACHU2L_000204"
     CDS             complement(35454..36551)
                     /gene="cysA"
                     /locus_tag="ACHU2L_000204"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR1355229.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(36541..37416)
                     /gene="cysW"
                     /locus_tag="ACHU2L_000205"
     CDS             complement(36541..37416)
                     /gene="cysW"
                     /locus_tag="ACHU2L_000205"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR1355230.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(37416..38249)
                     /gene="cysT"
                     /locus_tag="ACHU2L_000206"
     CDS             complement(37416..38249)
                     /gene="cysT"
                     /locus_tag="ACHU2L_000206"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR1355231.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(38249..39265)
                     /gene="cysP"
                     /locus_tag="ACHU2L_000207"
     CDS             complement(38249..39265)
                     /gene="cysP"
                     /locus_tag="ACHU2L_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR1355232.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(39569..40360)
                     /gene="ucpA"
                     /locus_tag="ACHU2L_000208"
     CDS             complement(39569..40360)
                     /gene="ucpA"
                     /locus_tag="ACHU2L_000208"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR1355233.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(40489..41347)
                     /gene="murR"
                     /locus_tag="ACHU2L_000209"
                     /pseudo
     CDS             complement(40489..41347)
                     /gene="murR"
                     /locus_tag="ACHU2L_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            41511..42407
                     /gene="murQ"
                     /locus_tag="ACHU2L_000210"
     CDS             41511..42407
                     /gene="murQ"
                     /locus_tag="ACHU2L_000210"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR1355234.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            42411..43835
                     /gene="murP"
                     /locus_tag="ACHU2L_000211"
     CDS             42411..43835
                     /gene="murP"
                     /locus_tag="ACHU2L_000211"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR1355235.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            43840..43926
                     /locus_tag="ACHU2L_000212"
                     /pseudo
     CDS             43840..43926
                     /locus_tag="ACHU2L_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44015..44914)
                     /gene="yfeX"
                     /locus_tag="ACHU2L_000213"
     CDS             complement(44015..44914)
                     /gene="yfeX"
                     /locus_tag="ACHU2L_000213"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR1355236.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(45010..45585)
                     /locus_tag="ACHU2L_000214"
     CDS             complement(45010..45585)
                     /locus_tag="ACHU2L_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR1355237.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(45646..46095)
                     /locus_tag="ACHU2L_000215"
     CDS             complement(45646..46095)
                     /locus_tag="ACHU2L_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR1355238.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(46082..46507)
                     /locus_tag="ACHU2L_000216"
     CDS             complement(46082..46507)
                     /locus_tag="ACHU2L_000216"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR1355239.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            46721..47590
                     /gene="amiA"
                     /locus_tag="ACHU2L_000217"
     CDS             46721..47590
                     /gene="amiA"
                     /locus_tag="ACHU2L_000217"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR1355240.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            47594..48493
                     /gene="hemF"
                     /locus_tag="ACHU2L_000218"
     CDS             47594..48493
                     /gene="hemF"
                     /locus_tag="ACHU2L_000218"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR1355241.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(48499..49551)
                     /gene="eutR"
                     /locus_tag="ACHU2L_000219"
                     /pseudo
     CDS             complement(48499..49551)
                     /gene="eutR"
                     /locus_tag="ACHU2L_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(49597..50097)
                     /gene="eutK"
                     /locus_tag="ACHU2L_000220"
     CDS             complement(49597..50097)
                     /gene="eutK"
                     /locus_tag="ACHU2L_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR1355242.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(50110..50769)
                     /gene="eutL"
                     /locus_tag="ACHU2L_000221"
     CDS             complement(50110..50769)
                     /gene="eutL"
                     /locus_tag="ACHU2L_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR1355243.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(50779..50847)
                     /locus_tag="ACHU2L_000222"
                     /pseudo
     CDS             complement(50779..50847)
                     /locus_tag="ACHU2L_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51951..52742
                     /locus_tag="ACHU2L_000223"
     CDS             51951..52742
                     /locus_tag="ACHU2L_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355244.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            53462..53857
                     /locus_tag="ACHU2L_000224"
     CDS             53462..53857
                     /locus_tag="ACHU2L_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1355245.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            53875..54138
                     /locus_tag="ACHU2L_000225"
     CDS             53875..54138
                     /locus_tag="ACHU2L_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355246.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            54161..54703
                     /locus_tag="ACHU2L_000226"
     CDS             54161..54703
                     /locus_tag="ACHU2L_000226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355247.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            54842..55129
                     /locus_tag="ACHU2L_000227"
     CDS             54842..55129
                     /locus_tag="ACHU2L_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355248.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            55168..55374
                     /locus_tag="ACHU2L_000228"
     CDS             55168..55374
                     /locus_tag="ACHU2L_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355249.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        13860 a        14738 c        13731 g        13219 t
ORIGIN      
        1 agtgcattat caagagatac attgctaata caccatttat cttttaatcc ccttttaaat
       61 ttatgttttt tcctcaaaaa cggcctgctg gtcatcagcc ttcaggttac ctatcataga
      121 ggtttaatcc ttattcagag tcacccgtgc acgaaatatt caacatgctg ctggcggtct
      181 tcgatcgggc agcgttaatg cttatctgcc tgttctttct catccgtatc cgcctgtttc
      241 gcgaactgtt gcacaagtcg gcgcactccc caaaagaatt gctcgccgtt accgccattt
      301 tctcgctgtt cgccctgttc agcacctggt ccggcgttcc cgtagaaggc tcgctggtga
      361 acgtacgtat tatcgcggtg atgtccggcg ggattctgtt tggcccgtgg gtaggcatca
      421 ttaccggcgt gattgcgggt attcaccggt atttaattga tatcggcggc gtgacggcga
      481 tcccctgctt tatcaccagc attctggcgg gttgtatatc aggctggata aacctgaaaa
      541 tccccaaagc acagcgctgg cgctggatat cgccaacaaa acgctaccgc tgtttcgcca
      601 tgtcaatagc gagtcattac gcaaggtctg cgaaattatc cgcgatgaca ttcacgccga
      661 tgcggtggcg attactaata ccgatcatgt gctggcctat gttggcgtgg gtgaacataa
      721 ctatcagaat ggcgatgact tcattagccc gactacccgt caggcgatga attacggaaa
      781 aatcatcatt aaaaacaatg atgaagccca ccgcacacca gagattcatt ccatgctggt
      841 gatcccattg tgggagaaag gggtcgtgac cggaacgctg aaaatttact actgccacgc
      901 gcatcagatc acctcgtcat tacaggaaat ggcggtcggt ctgtcgcaaa tcatctccac
      961 gcaactggag gtttcacgcg ccgagcagct acgtgaaatg gcaaataagg cagagattcg
     1021 cgccctgcaa agcaaaatta atccccattt tctgtttaac gctctgaacg ctatttcatc
     1081 gtcaatccgt ctgaatccgg ataccgctcg ccagttgatc tttaatctgt cgcgttatct
     1141 gcgctataac attgaattaa aagacgatga gcaaatcgat atcaaaaaag agctgtatca
     1201 aattaaagac tatattgcca ttgagcaggc ccgctttggc gacaagctga cggttatcta
     1261 tgatattgat gaagaggtga attgctgcat tcccagcctg ctgatccagc cgttggtgga
     1321 gaacgccatt gtccacggta ttcagccttg caaaggtaaa ggcgttgtca ccatcagcgt
     1381 tgcagagtgc ggaaatcggg tacgcattgc ggtgcgagat accgggcacg gcatcgttcc
     1441 aaaggtgatt gagcgggtcg aagcgaatga aatgccgggc aataaaattg gcctgctgaa
     1501 tgtccatcat cgcgtgaagt tattgtatgg cgaggggctg catatccgcc gcctggagcc
     1561 ggggacggaa attgcgtttt acattcctaa ccaacgcacc ccagtcgcct cacaggctac
     1621 gttattgctt tgagccggag tgatattgtg aaagtcatca ttgttgaaga cgaattcctg
     1681 gcacaacagg aactgagctg gctaattaaa gagcacagcc agatggagat tgtcggcacc
     1741 tttgacgacg gtctggacgt gttgaagttt ttgcagcata accgcgtcga cgccattttt
     1801 ctggatatca atattccgtc gctggatggc gtgttgctgg cgcaaaacat cagccagttc
     1861 gcccataaac cgtttattgt gttcatcacc gcgtggaaag aacatgcggt agaagcgttt
     1921 gaactggagg cgtttgacta cattctcaaa ccgtatcagg agtcacgtat taccgggatg
     1981 ctgcaaaaac tggaagcggc ctggcaacaa cagcagacca gcagtacgcc tgccgcgacg
     2041 gtaacgcgtg agaatgacac cattaatctg gtgaaagatg agcgaataat cgtcacgcca
     2101 attaacgata tctattacgc cgaagcgcac gagaaaatga cctttgtcta tacgcggcgt
     2161 gaatcctacg taatgccgat gaacattacc gaattttgca gcaaactgcc gccgtcgcat
     2221 tttttccgct gccatcgctc attttgtgtc aatctgaaca aaatacgcga aatcgaaccg
     2281 tggtttaata acacctacat tctgcgactg aaagatctgg attttgaagt gccggtcagc
     2341 cgcagcaaag tgaaagaatt tcgccagtta atgcatcttt aatcgggaac tttcatgaaa
     2401 gcaccaggct tgcccgccga tcaacaattt ttcgccgatc tgttcagcgg cctagtgctt
     2461 aacccgcaac tactggggcg cgtctggttt gccagccagc ctgcctcgtt accggtgggc
     2521 agtttatgta ttgattttcc ccgtctggat atcgtgctgc gcggcgaata cggcaacctg
     2581 ctggaagcaa agcagcagcg aatggtggaa ggagaaatgc tgtttattcc ggcacgcgcg
     2641 gctaatttac cgatcaacaa caaaccggtg atgctgttaa gcctggtgtt cgcaccgacc
     2701 tggcttgggt tatcgtttta cgatagccgc accacgtcgt tgttgcatcc tgctcgccag
     2761 acccagcttc ccagcctgca acgcggtgaa ggtgaagcga tgcttaccgc cctcacccat
     2821 cttagccgtt cgccgctgga gcagaatatc attcagccac tggtgttaag tttgctgcat
     2881 ctttgccgca acgtggtgaa tatgccgccg ggtaattcac aaccgcgcgg cgattttctc
     2941 tatcacagca tttgtaactg ggtttaggat aattatgccc agccgctcac ccgcgagagc
     3001 gtggcgcagt tttttaatat cacgcccaat catctgtcaa aactgtttgc tcagcatgga
     3061 acgatgcgtt ttatcgagta tgtgcgttgg gtgcgaatgg cgaaggcgag gatgattttg
     3121 cagaaatatc atctgtcaat tcatgaagtg gcacagcgtt gcggttttcc ggatagcgac
     3181 tatttttgtc gcgttttccg gcgtcagttt agtctgacgc cgggagagta cagcgcccgt
     3241 tttcagggct aacgtcagaa ggttaattct gtttccagca gcgtcaggat actttgtgca
     3301 tcttgcgcgg caaacaacga ctggcggaag tttttattca ccagtttacg cgccagctgc
     3361 gagaagactt tcacatgatt catcccttcg ttagcaccca gcgtcagcat gatcaccagt
     3421 tcgacttcgc ccatttctga ctgccagtca accggtttcg ccagccgggc aatgctgata
     3481 ctggaatgac ggatccactg agatttggtg tgcgggatcg ctacgccaaa accaacgccg
     3541 gtggtaacaa tctcttcccg ctgccagaca tcttcttcca gctcaaacgg atgttcagtg
     3601 cgcccgttaa cgccgaggtt gccgcacagg aactggatcg cctgctcttt attgctaaaa
     3661 tcctgatcaa caaagatatt ctccagcgcc agcagtgggc gaacgtcttc ttccggcgta
     3721 aaggcggtga gtaacgcttc aatttcctgc gcactgcggc attcacatgc ctgacgcgcc
     3781 agttcccgac acgcctcgct atccagttga cgaagctggc ttttcaccgc cggaatacgc
     3841 gggctactca tactcagctc atccaggccc agcccaagca gtagcggcag ataacggctt
     3901 tcaccgccca gttcaccgca aatgcctacc catttgcccc gctgatgcgc agtggtaact
     3961 atttgctgca acatgcgcag gaacgatggc gtaatcgggt tatatagcgg cgatacgcgc
     4021 gggttattac gatcgaccgc atacagatac tgggtcatat cgttggagcc gatactgaag
     4081 aaatcgacct catcgcagaa gtggtcgatg atgtagcaca ccgacggcac ttccaccatg
     4141 atcccaagcg taatcgtctc tgcatgacgc aggccatcgc gcttaagctc aacgatcgct
     4201 ttttggatct cgcctttcac ccataagatc tgatcgaggc tgtgaaccat cgggatcatc
     4261 aactgggcgt tgccgaaact ggcggcgcgc aaaatggccc gcagttgagt gcggaacagg
     4321 ccagcaaatt ccgggtaaat acgtaccgcg cgatagccga ggaacgggtt ctcttcctgg
     4381 ggaatattca gataaggaat gcttttatcg ccgccgatgt ccatcgtgcg gaagataatc
     4441 ggcttgtcgc ccgccgcccg ccgccagtag cacctgctgg taggcttcaa attgctcctg
     4501 ctcgtccggc gcgctgtcgc gatccatata gagcatttcg gtacggaaca gcccgacacc
     4561 ttccgcgccg ttggcaaacg cgcctggcgc ttccagagcg gtgccgatat tcgccgcaat
     4621 atcaatacgc ttgttatcac gggaataggc cagctgcgcg gcagcctgcg cctgttgttt
     4681 ttggcgttta tccgccagcg tctgcgcgac ctgataataa ccgctcaccg cgtcattcgg
     4741 gttaatcgcc agcacgccgc actgggcgtc aagcactgca ggttgcccgg cataacgggc
     4801 aatcgcatcc agcggcaagc cactcagtac cgggatcgcc gaggcacggg ccagaatcag
     4861 tgtatgcgag gtgcgcccgg ttttctccag aatcataccc gcaagatttt tcaaatcaag
     4921 gctcaaaaac tggcttgggg ttaaatcttc agccaccaga atggtcggtt tttccagcac
     4981 cagattgttg cgcggcttca gttccggcca ggtgatatgc agcaactgtt cgctgatatc
     5041 gcgaatgtcg ctgacccgct cgcgcagata atcgctggca gaagcagata gtttggcgca
     5101 aacctgctcc atattgctga tgatcgccgc ccccagtccc tgatgctgtt ctgtcatcag
     5161 gcgacggata ttgcctgcaa attcatcatc ctgaatcagc gacaaatgag cgctgaggat
     5221 agttttgctt tcgccgtcac gctcacgcaa ttgctgattc agttgctcgg caagcgttgc
     5281 caggctgtgc tccagccggg tggaatcttg cgcactggcg gggattgccc gataactgtc
     5341 gaggctgtcg ctttgtaaca gggtcagcgt acccacgccg acgccgcttg ccagcacatt
     5401 gccgtacagt aaatccgggt taaggcggct taatgaacgc ggcagcggat gcgccgtcag
     5461 ttctgcctgc gtcggctgaa cgctgtcgct gtcgataaag cgcaccagga tgtactcttc
     5521 cagcacgcgc cgcgcctgct cttcatcgct gccgctaatg ttcaggctgc aactgtcatt
     5581 aaataaggtg ccagttccaa tcaacgccag cgagctttta gcatcagcct ttgcgttctg
     5641 gcgatggttg ataaaggtga tttcgctttg ccactggctg cactgttctt taagttccca
     5701 cgccggacga gcatgtagac cgttaggcaa aggacagaga aattgaatcg ttaacataac
     5761 cgctccgggt taatcaggag atcatctgaa atccgtcagt tgaacaaccg tctcacgcgt
     5821 aagacgttga ataagggcag ataaaagttg ctccatctgc aaaatgtcgc ggcaatcggc
     5881 aatcggcaat cgatgccgcg caatgtccgt ggcgggtggc aggccccatt accaatgtgg
     5941 gtacgccagt accggttaag tgcaccgctc caccgtccgt gccgccgtta ctgaacatat
     6001 ccgcctgcaa cggaacgcca atttctgccg ccacggtttc aatccacgcg gtaagttttg
     6061 gcggcgcaat cagcgattta tcgcttaaca ccagcatcgg cccgttacca atctgacgat
     6121 ggttagccgc gccataatca aagtttttcg cccagcaggc ggtatcaagc acaatggcga
     6181 cgtccggcga caccgcgcgg gtagcagttt gcccgccgcg taatcccacc tcttcgctgg
     6241 aacttgccac cagccacact tccgcaggca gttcggcgtc gtgcagctca cgtagtaacg
     6301 tcaccagcag atagcaactg aggcgatcat caaaggcttt gcccatcacc cgctggtgag
     6361 ggaggacctg aaaagtggta tcgaaggtga cgcgatcgcc tggacgaatt cccgcctgca
     6421 tcacttcgtc ataggtgcgc gcgccaatgt ccacgcgcat ggcgctaacg tcattcccct
     6481 gccggtcgcc gtcaagcagg cctggaattt tgcactcttc acgggtggtg atgcgcaccg
     6541 gctgcagctg gcgggcagcc atgcgcacgt tgccaaccgg cagcacgtca atagcgcctt
     6601 cgcgggagat gctgcgcacc ataaatccca cttcgtccat atgggcgcag atcatcacct
     6661 tcggacctgt cgattcattg aggcggatca gcaccgatcc cagaccatca aatcgcactt
     6721 ctttttgcag gcgagccgct tcttccagca ggatctgccg cacttcctgt tccgaggagg
     6781 cgatcgcatc tgcctcgctc aacgctttta atagcgataa atccattatg cctctcccgt
     6841 gagcaacact gttttcggca tggcgtagag cacttctgcg ccttgcgggg taaccagcac
     6901 aacatcttcg atgcgcacgc ccccttgccc tagcaaataa atccccggct ccacggtcag
     6961 caacatgcct ggctgtagcg tcgtggtgtc ccgcggtgaa aaacgcggat cttcatggac
     7021 ttcaatgccg atagcgtgac cagtgttatg accgaaatag tcgccaaatc cagcctcggt
     7081 aatgacccgg cgcgcggcgt cgtcaatctg ctggcagcgc acgccggggc gaattgcaga
     7141 gattgccgcg agctgtgcct gcaggacaat ctgatagacg tcaaacagcg ggtgagattc
     7201 ggcgctcacc ccttcgccat tcaccagcaa ggtgcgcgtc atatcagcag tagccctgat
     7261 acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc aacaatcttg tcgctggctt
     7321 tgccgtgcgg cagcgccccg cgccagccgc tggcgacaat ggtgtcaaaa gaggcttttt
     7381 ctgcgccctg ctggcgcata aaccactcca gttcagcggc tatctcgcgc tcgctcatcc
     7441 ccgcctgaat aaagcggcga atatgctccg ctccgcgatc ggcaatccca caggcgaggc
     7501 ggattatctc cacctcctct ggcgttttga tttgccgcag cacatccggc gtggcgctaa
     7561 ccagtttcgc attgagttca gacttccagc ggtgcgcggt ttcccaactc acctgctggc
     7621 cttcaaaacc gagcgtttgc aactgctcat cggcaatgat ttgattgacg atagtggtaa
     7681 gcgtgttcgt tgcgtcaagc aaatgcagct ggtagccttg cgcacgggct tccacctcaa
     7741 cgaaatagcg cgaatctacc agaatgtgcg cactttcacg gctaataacc acatagcctg
     7801 atccggtgga gatccccaga tgcggctgtt tgttctgccg tgaggagaga agcaccgcat
     7861 ccagttgttg caccttaagc cagttgcgca gcgaagcgag taatgtcatc tggattttcc
     7921 ttcttacagg ctatcgatta acaatttgcc tttgcggaac atcatcagac gcaggaacac
     7981 caccatcagc gcagtaatga ttgcccccag cgcgatcccc gccatataaa cgcccaagtt
     8041 agtgaccagc ggccatgccc agatagcgga ttccgggaac cattgcactg cgcccagcca
     8101 gacagctgcg gttgagccga caatcgcgcc gaccatatat gacggaatag cggtgatggg
     8161 gctttccagc gcaaacggaa tcgcaccctc actaatcccc ataaaggcca ggaacatcgc
     8221 ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt ttgccggtta aacgacggtc
     8281 aataatggtc gccagaccca gaccaatcgg cggaataacg atagcgatag aacgagcggt
     8341 gaccggtaat acgtggtcag tggtaaagct gaaggcaacg aaacctgcgg ctttgttgat
     8401 cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca tcagcgcgcc
     8461 cttctcacct gcggcagtca gcacagtacg gataccgccg ttgatccagc caccaaaggg
     8521 agtgatgacg tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg gtagcaaaaa
     8581 tgtggttttg aacgctaaca ggaaatccgg taactggatt ttctggttca tccatttcac
     8641 aaggtaaccg gcaacgatag aaataatcag cgcaccaatg aaggtggacg gcaccggcga
     8701 agaggtcgcc cattgcatag tgctgggatc gaagttcagc agttgcgtcg gctgggtaga
     8761 catcaacccg ccgataaaac cagccgggaa cgccagtttg ccaccgatag agttcgccac
     8821 aaatgcagca aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg actgcgacag
     8881 ccaggcaaat ttaagtaagg agaggtcgaa accggagaat ttcccgctat taagagcatc
     8941 catgatgccg atctcagcag gaattttaag ccagctataa gcaatcagct ggctgaaagc
     9001 gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg acatcacatg
     9061 ctgcgggagt tcaccccaga agctgctttt agaggcctgc ggattcttaa ctgccgctgc
     9121 cgcaccggat gcgccaggca caacggttgc actgcgtttt ttaatggcca taacaattat
     9181 ccctgtcgaa tattactgtt gttcagaggc aatcatctct tcgatttctt tgatgatgcc
     9241 cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg attcgaaacg
     9301 ttcgttatct tccggggtaa ctgccacgga atggatgatg atggtcgctt cggcgatatc
     9361 ctgcgccgtc aggcgattct ggatgccgtc cgcgccctgg gtttcaattt tcacttcata
     9421 accggcttct accgccgctt cttccagcac ctgagcggcc ataaaagtgt gagccaggcc
     9481 cattgggcag gcacataagg caatcagttt cttactcatc gttaattcct cgttcagtga
     9541 tagttgacta gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga caaataatgc
     9601 atttactgga gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc ttgtgagggg
     9661 acacaaagaa aaattgaatt aactgtatga tttaaaagat tatcgggaga gttacctccc
     9721 gatataacag gaaggattta cagaatgtga cctaaggtct ggcgtaaatg tgcaccggaa
     9781 ccgagaagac ccggatagtc atggacgatg agatacaccg gaatatcatg gacatattct
     9841 ttaaagcgcc ctttatcttc aaatgcggca cggaaaccgg aggctttgaa gaactcaagg
     9901 aagcgcggca cgataccgcc cgcaataaac acgccgccaa atgtcccgag attgagcgcc
     9961 agattgccgc caaaacggcc cataatgacg caaaacagcg acaatgcgcg gcggcaatcg
    10021 gtgcagctgt cagccagcgc gcgttcggta atatcttttg gcttgagatt ttctggcagg
    10081 cggttgtcag ctttcacaat tgcgcgatac aaattcacca gcccagggcc agaaagcacg
    10141 cgctccgccg aaacatgacc aatttccgca cgcaatattt cgaggataat ggcctcttct
    10201 tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac ccaacgctta
    10261 tcgacatgga ccagatgcgc aaccccaagc cccgttccgg caccgtaaac cgcaataggc
    10321 ttaccttcga ccggttctgc gccaccaaac tgaatcagat gctctttttt cagcatcggg
    10381 atcgccatcg atacagcggt aaaatcgtta ataatttcca gatggctaaa accgagattc
    10441 tttttcattt cggcaattga gaacgcccag gtatggttgg tcattgccac ccagtcaccg
    10501 gtaattgggc aagcgatggc aatacagccg tctttcacct cgaccttatg ttcttcaaga
    10561 taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc ctgaataggt cttagcctgc
    10621 gagatttcac cactggcaat atcacacaga gcaagacgtg cgttggtgcc gcccacatca
    10681 ccgactaatg catactttgt cattcttcaa ctgctccgct aaagtcaaaa taattctttc
    10741 tcacactgta aatacctggg ggcataacaa caacgccgga aaggcaggct ccctgtaaat
    10801 atcgatctgg gtcacacaat tactttatcg tttcagcacc aattgcagcg atgccttttt
    10861 gcaagctggg caaactaagt atctgacccc gcataaggaa tagaacatgc tccatccgcg
    10921 agccagaacc atgttgttat tatcgctccc cgccgtggca attgggattg cgtccagtct
    10981 tattctgatt gtggtgatga aaatcgcctc ggtattacag aatttgctct ggcaacgact
    11041 gccgggaact ctggggatag cccaggattc acccctctgg atcatcggtg tattaacgct
    11101 aacgggtatt gcggtggggt tggttatccg tttcagccag ggtcatgccg gaccagaccc
    11161 cgcctgtgaa ccgctgatcg gcgcaccggt tccgccctct gcgctaccta gacttatcgt
    11221 agcattaatt ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc cgatcatgac
    11281 cgtcaatatc gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg tcaaccgaat
    11341 ggagtggact attttagcct ctgccggaac catcggcgcg ctgtttggta cacctgttgc
    11401 ggcggcgttg atattttcgc aaaccttaaa tggcagtagt gaagttccgc tatgggatcg
    11461 tctttttgcg ccgttaatgg cggcagcagc tggtgcactt actaccggat tatttttcca
    11521 tccccatttt tcactgccca ttgcgcatta cggacagatg gaaatgaccg atattctcag
    11581 cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat ggtgcttacc
    11641 acggttgcac gcgatgatga atcaaatgaa aaatccggtg ctcgtgctgg gtattggcgg
    11701 atttattctc ggaattctgg gggttattgg tggaccagtt tcgctgttta aagggctgga
    11761 tgagatgcag cagatggtgg caaatcaggc tttcagcacc agcgattact ttttgctggc
    11821 ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg gtgggcgaat
    11881 cttcccggca gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc acgttcccgc
    11941 cgttccagcg gcaataaccg tttcctgcgc tattctcggc atcgtgctgg tggtaacacg
    12001 cgatggctgg ttaagtcttt ttatggcggc agtcgttgta cccaatacca cattgctacc
    12061 gctgctctgt atcgtcatgc ttccggcatg gctgttatta gcaggtaagc cgatgatgat
    12121 ggtcaatcgt ccgaagcaac agccacccca cgataatgtt tagcaaaaaa tgctctcctt
    12181 tatgatttaa gagttatggc gatgatacgg ggctttacct ccccgtaata ttgcgttaac
    12241 aggccgctga cggcttcgat cgtgaaggag aataacaatg ttcaggtcac tgtttctggc
    12301 ggccgccctg atggcattta ccccgcttgc agcaaatgca ggtgaaatca ccctactgcc
    12361 atcgatcaaa ttacaaattg gcgatcgcga tcattacggt aattactggg acggtggtca
    12421 ctggcgagat cgtgattact ggcatcgcaa ttatgaatgg cgcaaaaacc gctggtggcg
    12481 tcatgataat ggctatcatc gtggctggga taagcgtaaa gcatatgagc gtggctatcg
    12541 tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg ggcaccgcca
    12601 ttaaacgcgt cctccaatgg agcacaatgc ctgatgcgac gctagcgcgt cttatcaggc
    12661 ctacaaaccg gctctttaac gcgtagggcg gataaggcgt ttacgccgca tccgccaata
    12721 gtgccagatg cgacgctcat tcaactacaa tcccagcgcc gtccccacca acaaccagat
    12781 attcagcgcc acgaccagca ctacaatcac ccagcctgtc tgttttacgc gtttgctgtt
    12841 caccagatcg cccatcaact tgctgtcact ggtgaaaatc agcagtggaa ccagcgccag
    12901 ggcgatacca aaacttaaca gcacctgact cataaccaga atccgtgtcg gatctaatcc
    12961 catcagaatg acaataaatg acggcaacat ggtgactgta cgacgcaccc acagcgggat
    13021 atggaagcga atgaatccct gcatcaccac ctgccccgcc agtgtcccca ccaccgttga
    13081 ggacagtccg gcagcaacca gacttaaccc aaagaccgtt gccgcagcat ggcttaacag
    13141 cggttgcagc gtcagataag cctcatcaag atcggcaaca ccagtatgac cagaaaagtg
    13201 gaacgccgcc gcagctgtag ccatcatcac cagattgaca aaaccggcaa tcgtcatggc
    13261 gatagccaca tcccatttgg tggcggaata acgttgttga cgcgaaccgc catgtaaatg
    13321 ctgagtgagc gaagagtgca aataaatcac atgcggcata atcgtcgccc ctaacacgcc
    13381 tgctgccagg aagaccgcct ccgaagtagg taaactcggg atcaccattc ctttacccag
    13441 ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg caacaaacaa
    13501 cagtaacccg ccaatcactt tctccagcgg tttttgcccg cgacgttgca gcattaaaat
    13561 caggaaagtc gcgatccccg tcagcaccgc gccctgcaac aacgaaacac caagaatgag
    13621 tttaaaaccg atcgccgcac caataaattc cgccaggtcg gttgccatcg caataatttc
    13681 tgcctgaacc caatagaacc acactacggg acgcggatag tgatcacgaa tctgctccgc
    13741 cagattttta ccggtggcaa tccctagttt ggcagagagg atctgaatca gcatcgccat
    13801 caggttggcc caaacgacaa cccacagtag ctgatagccg aagctagcac ccgcctgaat
    13861 attggtcgca aagttaccgg gatcgatata accaatcgcc gcaatgaacg caggtcccat
    13921 taatgcgagc ctcatcttac gcgccgcccg tccgctgcta ctctcaacgc gatagttcgt
    13981 catcttgtgc ctctaaaaca tagcctttgc tatgtttcat gctatgctaa acgagaatga
    14041 ttatcaaatt catttaaatg gattgtggtg atttctctga tagaccaaga ttatgattac
    14101 gaaaagattg atgacttgaa tgttatggtt atgtttaatg ttagcacatt tacataactt
    14161 tcagcttcca tacacaacat agcagaaatg tatgacagat cactattttt gaagcctgtc
    14221 acaggacgtc attatagtgt gtgtcagatc tcgttttcct taaccatgtt acatagaatg
    14281 tgcacggaaa tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc cttcattttg
    14341 tactggcact tgccgttgtt gtgattctcg cactgctggt aagcagcgac cgcaaaaaaa
    14401 ttcgtatccg ttatgttatt caactgcttg ttatcgaagt gttactggcg tggttcttcc
    14461 tgaactccga cgttggttta ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc
    14521 tcggatttgc caacgaaggg actaacttcg tctttggtag catgaatgat caaggcctgg
    14581 cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg atcggtattc
    14641 tccagcacat tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg ctctccaaag
    14701 tcaacggtat gggcaaactg gaatccttta acgccgtcag ctccctgatt ctgggtcagt
    14761 cagaaaactt tattgcctat aaagatatcc tcggcaaaat ctcccgtaat cgtatgtaca
    14821 ccatggctgc cacggcaatg tccaccgtgt cgatgtccat cgttggtgca tacatgacca
    14881 tgctggaacc gaaatacgtc gttgctgcgc tggtactgaa catgttcagc acctttatcg
    14941 tgctgtcgct gatcaatcct taccgtgttg atgccagtga agaaaacatc cagatgtcca
    15001 acctgcacga aggtcagagc ttcttcgaaa tgctgggtga atacattctg gcaggtttca
    15061 aagttgccat tatcgttgcc gcgatgctga tcggctttat cgccctgatc gccgcgctga
    15121 acgcactgtt tgccaccgtt actggctggt ttggctacag catctccttc cagggcatcc
    15181 tgggctacat cttctatccg attgcatggg tgatgggtgt tccttccagt gaagcactgc
    15241 aagtgggcag tatcatggcg accaaactgg tttccaacga gttcgttgcg atgatggatc
    15301 tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg catcatctct gtgttcctag
    15361 tttccttcgc taacttctct tcaatcggga ttatcgcagg tgcagttaaa ggcctgaatg
    15421 aagagcaagg taacgtggtt tctcgcttcg gtctgaaact ggtttacggc tctaccctgg
    15481 tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta aaaccataca taaaaaagcc
    15541 ggggatcatt ccccggcttt tttacgcctg ttaatcagct aatggctgcg ggcgaccaat
    15601 caaataccct tgcagatatt gcaccccgag cttatgcaat agcgcctgct gctgttgcgt
    15661 ctcgacaaac tccgcgacca cactcaatga cttcgctttc gccagatcgg taattgatct
    15721 cacaatcatc gcatccagcg tattcgtgac aatatctttc acaaagacgc catcaatttt
    15781 gatgatatca gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg tgccaaagtc
    15841 atcaatcgca atccggaaac caaacttatg cagctgctcg atgttgtaca tgctgctttc
    15901 tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg accgcctgcg gggagatgtg
    15961 ataacgctta aacagacgaa taatccgccc ggcaatattc ttttgcagca gcgtgagcgg
    16021 cattaaattg actgaaaagc gcggtccttt tttgtcgcaa gggtgtgttg ccagccactt
    16081 caacaaggat tccagcactt gcaaatcaaa acgcgcgcta aggttaaact gagcaataag
    16141 gggcagaaac ttatccgggg tcataatgcc gccgtcatat ttcagtcgcg cgaggatctc
    16201 atcataacct tcaccctctt tgttgcgaat tggctgggcg tagagcagca aatcaccctg
    16261 atctaacgcc gtgcgaatgg tattcagcaa tagcacctgt ttagtggtct gcccggaaac
    16321 catctcctct ctgctatcca gcgccagcac atgatgatgt gcgcaggatt gctccgccag
    16381 ccagcttaac tgccccaaca agggttgcag ggtttcctga tttccatcaa aacgccccca
    16441 ggcagcacca tagcccatat ccagcccggt attgttccag tgaatttgcc gactattcag
    16501 gatgttaacc atatgctgga gtcgcccttc cgtttccggc ccgctcagca ctaacagcag
    16561 ttcactaccc ggcaattgat acaacttttc gttttcctgc atcaacggca gcagcgtacg
    16621 gcaaattgag cggatacagt gaacgcgcat cattaagccg taatgacgac tcataaactc
    16681 aagattatca atgcgcaggc agcaaaaact cttgcccgcc tcttgctccg gcgcttgttc
    16741 caacgcacga aagttgggta ggagcgttaa cggatctgtt agcgcctgca aatgccagcg
    16801 gcgattaagc cattcactgc gatgataaat gcgcaccata tagagcaggc aaacgctaaa
    16861 ggaaatcagc accgcgagaa taaatgccag cgaatattcg gtttccaccc cttgcaaaaa
    16921 gttctgattg taattcagaa ggcaaagcgt tgaaacagcc caggtgagat ttaaaaacgg
    16981 atagcgaaga ttaccgaccc cgagggtaaa gatgatgaag aaaacgggta ccaggtaacc
    17041 ggcaataaag tcgttttcat aaggtgtgca caacagaagt agcagcacgc ttaacgctgc
    17101 cagccagctt aaggtaaatg cgagtgagat agtagaaaag catgttgtaa atcagcactg
    17161 cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg ccgaaaaagg
    17221 tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg catttgatgc
    17281 cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg ctggtcaatc
    17341 cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac aacacgacaa
    17401 acgtctggct gccgagcatg accgcagtct gtaaaaaaga gagatgaaag ttccacagat
    17461 tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag ataaacaaga
    17521 ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa tggataatgg
    17581 caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta aacgctagtg
    17641 tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca ctactctcac
    17701 tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag caaacagggg
    17761 ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct aacccggacg
    17821 tagactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt tgcagcgagg
    17881 gttggaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag
    17941 cgctctccca gctgagctat agccccacga tgcgtttacg taccaaattt gctgggtgca
    18001 aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc
    18061 tcccagctga gctatagccc cgtcacgtaa agcttgtcga gttgacgggc ggcatcatat
    18121 gaattccgcc cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc gctgaaaaat
    18181 caggcaaatg aacaattttg gaaaaccgct cgcattcagt agttattcat gtcacggttt
    18241 cctgtaaagt ggtgttataa aatgaactac taatagaccc acatacattc agggaattgt
    18301 tatgttcaag gagaggatga caccagatga acttgccaga ctgaccggct atagccgcca
    18361 gaccattaat aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac ctggcgtcca
    18421 gggtggcaaa gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata ttcgcaatgc
    18481 tgaacgtcca gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc cgcttgaagt
    18541 attgctggtg acactggcga aagagatgac gccagttgaa caaaaacagt ttacatcctt
    18601 gcttctgcgg gaagggatta tcggattgtt acaacgctta gggattcgcg atagcaaata
    18661 atatgaaaag attacgcaat aaaatgacca ccgaagaact ggctgaatgc ctcggtgtgg
    18721 ctaaacaaac tgttaaccgt tggaccagag aaaaaggctg gaaaacggaa aaatttcccg
    18781 gcgtgaaagg cggtcgtgcc agacttattc tggtcgatac gcaagtttgc gagtttattc
    18841 agaacacgcc agccttccat aacacaccaa tgttgatgga agctgaagag agaattgctg
    18901 aatacgctcc tggagcacgc gccccggcgt atcgacaaat catcaacgcg atagataata
    18961 tgacagacat tgagcaggaa aaagtcgcgc aatttttgtc acgtgaagga attcgcaatt
    19021 tcctcgcccg tctcgatatt gacgaatcag cataaataaa aaacggcagg atattatctc
    19081 ctgccgttta tctttttaca cgctaattac tgctgatttt cgcgttcagc aataaaatcc
    19141 agcgctttgt tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac ggtaacatcc
    19201 agtgctggag actgccccgc accggttacg gcgacacgca gcggcatacc aactttaccc
    19261 atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt ttcagcggtc
    19321 cagtcagtaa tcgcggccag tttgtcacga accacttcca gcggctgacg cgctaccgga
    19381 cgcagatgtt ttttcgccgc atcggcatcg aactcagcaa aatcttcgta gaaataacgg
    19441 cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt caccagatca
    19501 gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca ctgtaagtga
    19561 gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca cagcagcttg
    19621 tcggtgttga acgcactggc agatttgctg acggcattca aagtgaagta tttgatcatc
    19681 tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac cagatagttc
    19741 agcagtgctt ctggcaaata accgtcatca cgatactgca ttacgctgac tgccccgtga
    19801 cgtttggaca gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc gtaaaccggc
    19861 accggcgctt tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt gatatggtct
    19921 tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac acagaagtta
    19981 taggttgggg aaccatcggt acggcggata ataagatcgt ccagttcctg gttgctgaac
    20041 tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg gttagcaaaa
    20101 cgtacaacac acggttcatc atcagcatga tgctcatggc tgtggcggca gcgaccgtca
    20161 taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag gcgctcttta
    20221 gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac cgcgttgtag
    20281 cgatcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct cagccagttc
    20341 atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc ggtgtcttca
    20401 atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta aagagcagta
    20461 cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt tttgattttc
    20521 atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga aaaattgagt
    20581 gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc taccttacgc
    20641 attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat cttgcttaaa
    20701 tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa ctctccctat
    20761 aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc ccttacaagg
    20821 agggggtcgg cggttcgatc ccgtcatcac ccaccaacta ctttatgtag tctccgccgt
    20881 gtagcaagaa attgagaagt gggtgattag ctcagctggg agagcacctc ccttacaagg
    20941 agggggtcgg cggttcgatc ccgtcatcac ccaccacttc gggtcgttag ctcagttggt
    21001 agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac ccaccaatgt
    21061 aaaaaagcgc cctaaaggcg cttttttgct atctgcgata ctcaaagatt cgaacctgca
    21121 gcaggtttga gttgagcgca gcgaaacaac ggagccgctc gcggcgacgg cccgaagggc
    21181 gagcgaagcg agtcatcctg cacgacccac caatgcaaaa aagcgcccta aaggcgcttt
    21241 tttgctatct gcgatttgta agattgattt ttcttctcaa gttccttgtt cttctaccga
    21301 tattacctcg tacagtgacg aggactgaac aatggccact atctcttcaa cttcaattcc
    21361 ttccatacaa acacagagta gtaacagggc atcacaaggt agcgatgttg cttcacaaat
    21421 cgcgcggata agtcagcaaa tcatcaaact cactcaacaa attaaagaga ttgtagacac
    21481 tagcggcagt gctgaagaca agcaaaaaca ggccgaattg attcaacagc aaatcaccct
    21541 actggaaaca caactggcgc agttgcagaa acagcaggca gaaaaggcgc aggagaaaga
    21601 gcagcgtttg tctctgaatg tatcccttct aaatcctgtt gaaaaaacga ctcatatcga
    21661 tatctatatc tgatacaacg gatccccttc ccgaccagcc attagcgcat tgccctggtc
    21721 tcgtaccacg tcccataacg cttcagcggc ggtggaaagt gaacggttct tcctgcgcac
    21781 cagcatcaac tggcgttcaa ccaccggcgt aatgcgctta accaccagtg ggctaccttc
    21841 aggcaatggt agcgccagtg caggaagaat actgatgccg atgcctgcgg ctaccatcgg
    21901 gaacagcgtc gccggatgtc caatctcctg cacaatattc gcctgaatac cattgcgcgc
    21961 cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca ccaatttcgc
    22021 tccctgtaat gcctgccagg gtacataatc ttccacggcc aaagcgctat cgcgatggca
    22081 aagcagaaag aaaggctcgg aaagaatcgc ttcacattgc agatcgccca ctgggcctgg
    22141 atcgatgacg atgccaaagt cgacatctcc ctggcgaata ctttccatca cccactgctg
    22201 cggacgatca tgcaggacaa actgaatatc tggatagcgg cgatggcttt cagcaatgca
    22261 ttgcggaata agatgcgcgg aaatggtctg gctggcagcg acccgaactt ttccgctcag
    22321 ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca gcagtcgctc
    22381 aagacgcaaa gccaactgct gccctgcatc tgtaagcacc acttcacgcg tggttctgtc
    22441 cagcaggcga acaccagtat gattttccag ctccttcaca ctgtgactta ctgccgactg
    22501 gctcaagccg atacgctctc ctgcacgact aaaacttttc tcctgcgcta ctgtgacgaa
    22561 aacctttaat tgttttaaag aataattcat ctgttttctt catgaatgga tgcaataaat
    22621 caattttatt tctcaaactg gaagaagcac aatagaacca tcgatcatct ggagtcttta
    22681 tgaaactttt tcgtatcctc gatcctttca ccttaaccct gatcacggtg gtgttgctgg
    22741 cctctttctt tccggccaga ggcgatttcg tccccttctt tgaaaatctg accaccgcag
    22801 ctattgccct gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg attattgctg
    22861 gcggtggtca ctggcgactg catttgtggg taatgtgcag caccttcgtg ctgtttccga
    22921 ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt cgacccgatg ctctactccg
    22981 gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc tgcaatcgcc ttcacgtcaa
    23041 tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc agcatccagc ctgctgggga
    23101 ttttcctttc accattgctg gttggtctgg tgatgaatgt tcacggtgca aggggcagcc
    23161 ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc gtttgtgttg gggcatcttt
    23221 cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa atggattgcg aaaactgacc
    23281 agacgtccat tctgttggtg gtttatacag cgttcagcga agccgtcgtt aatggtatct
    23341 ggcataaagt tggctgggga tcattgctgt ttatcgtggt ggtcagctgc gttcttctgg
    23401 ctatcgtgat tgtagttaac gtctttatgg cacgccgact gagcttcaat aaggcagatg
    23461 aaattactat cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc ccgatggcaa
    23521 acattctgtt ccccacatcg gtgatcggta tgatggtgct gcccctgatg attttccatc
    23581 agatccaatt gatggtctgt gcggtgctgg cgcgtcgata caaacgccag accgaacagt
    23641 tacaggcgca gcaggaaagc agcgccgata aagcttaaag cggacgcttc aggggctgga
    23701 ccagttgcgt cagcccctcg gttttaatca gcagcgtgat ttgcattagc tcaccgagct
    23761 tccctgccgg gaactcatct ttgcgggcaa accacagcag atactcctcc ggcaggtcaa
    23821 ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt attggctatt tcaatcagct
    23881 gctctttttc catctcagct acccagcaaa cgcagcattt ccgcttcgtc gatgacttca
    23941 atgcccagtt cctgcgcctt cgccagttta gatcctgcag cttcacccgc tatcaccaga
    24001 tcggttttct tcgacacgct gcccgcgact ttcgcgccca gttcgaccag tcgagcttta
    24061 gcgtcatcac gcgacatctg gcttaagctg cccgtaagca ccacggtttt accagcaaac
    24121 gggctgtcaa tctcttccgc gttgataacg atcggcgcag gccagtgaac accttccgcc
    24181 aacagctcgc tgatgacatt gcggttgctt tcttcggcaa agaagttgtg aacgtgggat
    24241 gcaacgacaa tgccaacatc aggcaccttt tgcagctctt caatcgaagc ggcttccagc
    24301 gcttccagcg tgccgaaata tgccgccaga cctgctgcgg tggcctcgcc gacttcacgg
    24361 atgccaagtg catagaggaa gcgagcaaag gtggtttctt tcgctttttc cagcgcgtta
    24421 accacgtttt gtgccaattt tggccccata cgctccagtc cggtcagttt gcctgcggtg
    24481 agtttgaaca gatctgccgg agtatggaca tattcttttt caaccagctg atcgatgatt
    24541 ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa caaagtgttt cagcgactct
    24601 ttacgctgcg caccgcaaat caggccaccg gtacagcggg caaccgcttc accttccaca
    24661 cgctcaacgt cagaaccaca taccggacaa tgcgtcggga atacaacctc acgggtatct
    24721 tccgggcgtt cagaaagcac gacgttaacc acctgcggga tcacgtcgcc agcgcggcga
    24781 atcaccactt tatcgccaat gcgtaaacca agacgttcga tttcatccgc attgtgtaag
    24841 gttgcgttac tcaccagcac gccagcaaca tggacaggtt ccagacgcgc aacaggcgta
    24901 atcgcgccag tacgcccaac ctgaaactcg acgtcacgca caaaggtcat ctgctcctgc
    24961 gccgggaatt taaacgcaac cgcccagcgc ggggcacgcg cgacaaagcc aagctgctcc
    25021 tgctgttcca gtgagttgac cttaatcacc acgccgtcga tatcaaagcc cagcgtcggg
    25081 cggtcttctt ccactttgtg atagaacgcc agcacttctt ccgccgattc acaaagcgtt
    25141 acccgatcgc tgaccggcac cccccacttt ttaaattgca gtaaacggcc aagatgagta
    25201 tccggcagct cgccaccttc cagaacacca acgccatagc agaaaaaagt gagcggtcgc
    25261 ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag ctgccgcatt acgtgggtta
    25321 gcaaaaactt tcccgcccgt gcgtcgcgca tcttcgttaa tcttttcgaa ccccgcctgc
    25381 ggcaggaaca cttcaccacg cacttccaga cgcgccggga tattctctcc gtgcagcttc
    25441 agcggaatgg cgcgaatagt acgcacatta gacgtgatat cttccccggt ggtgccatcg
    25501 ccacgggtcg cggcactgac taaaacgcca ttttcataca gaatactgac ggcaaggcca
    25561 tccagcttca gctcacagca ccaggtgact ttctcgttgt ttttcagacg gtcctgcaca
    25621 cgtttgttga aagcaagaaa gctttcttca tcaaaaacgt tatccagtga cagcattggt
    25681 acttcatggc gtatctggct gaaagctgcc agcggcgcag cacctacacg ttgggtaggc
    25741 gaatcaggcg taatcagttc tggatgtttg gtttccagct cgcgcagttc gcgcatcagc
    25801 ctgtcgtatt cagcgtcggg aatttccggc gcatccatca catgataaag atattcatga
    25861 tggcaaagcg tcgttcgcag ttctgtcagt tgttgttcga ttgattccat atcgcaccat
    25921 caatgctaaa aacccccgac aagcgggggt tcgaagagga gttaatttgc cttaagtgta
    25981 tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg tactcgcgca atttctgcgg
    26041 agtcatcata cggcgctgat cgtcaagcac gacaccgccc acttcatcgg caatatgctg
    26101 cgcagattgc agcatcagct tgaagttctg cagctcgtca ccgtaagacg gtacctgcat
    26161 aaagatagtg acacccggag tagtgaaatc cttcatttca ggatcaaagg ttcccggttt
    26221 caccatattc gccaggctga ataacgccgg gccgctgcca tccgggctaa gatgacgatg
    26281 gtaaatattc atatcgccaa aaatgaagcc cgcttgttga atgctgttaa gaagcagttc
    26341 accgtttagc tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg cttctttgcg
    26401 cttcggttta tccataactg gagccggttc cgctacaggc tcaggctgtg gtgccgctac
    26461 gggttctgca ggctggaaag cctgttgtgc cggttgcggt gctgaatgca caggctgcgg
    26521 cgctggcgcg acctgtggcg aaactggctg ctgcaacggc tgttcaggct gcggctgata
    26581 ggcaggctgc tgcaccggct gcgctggacg cggagcatgt tgcggcggta cctgcgcttc
    26641 aggcggctgc tggaccggtt gacgcggctg cgcagacgca taaggcggta ggtactggtg
    26701 ttgcggcgac ggacgagcag cctcatgctc ctgagcgtta gccggggcat gattcacgcg
    26761 gtgaacacga acctcaccaa cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc
    26821 gtcacgtttt gacttcattc gttttaatgg ccgatcgcgg aacatagaag atcgttcttt
    26881 acggctggtc cagaaaccat gtaccagtaa agcgattatg gcgatcgcgc caacaatgat
    26941 taatatcaga cgcaaatcct gcatcattat attctctgtt gttctaacac cttgccacca
    27001 cggcaaacat ttactcacta agagtatttg ccgattacct caagtgcaag tgcactatta
    27061 actttcacag cacaaagata gatgaaatcg tgctttttgc tgttttttcg aacatatcct
    27121 aactgtccat tgcgcaatta cccggtaaaa tacgcagaat tttcctggga ttggtcaaaa
    27181 ggagctcatc ctgattatgg tttcatcatt cacatctgcc ccacgcagcg gtttttacta
    27241 ttttgcgcaa ggctggaagc tcgtctcgca acctgggatt cggcgtttcg ttattttacc
    27301 gctgctggtc aatattttgt tgatgggggg cgcattctgg tggctcttta cacagctcga
    27361 tgtctggatc ccgactttca tgagttacgt tccggactgg ctacaatggc tgagttatct
    27421 gttgtggcct ctggctgtca tctctgtgct gttagtgttt ggctatttct tctccacgat
    27481 tgctaactgg attgccgctc cgtttaacgg tttattggct gaacaactgg aagcacgatt
    27541 gactggcgct acaccgccag ataccgggat tttcggtatc atgaaagatg tgccgcgaat
    27601 catgaaacgc gaatggcaaa aatttgtctg gtatctgccg cgcgcaattg tattactaat
    27661 tctttacttc atccctggta ttgggcaaac cgtcgcgccg gtactgtggt tcctgtttag
    27721 cgcctggatg ttagccatcc agtattgcga ttaccccttc gataaccaca aagtgccgtt
    27781 taaagagatg cgcaccgccc tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt
    27841 aaccagcctg tttacgatga tcccgctgct taatctgttc atcatgcccg ttgccgtttg
    27901 tggcgcgacg gcgatgtggg tcgattgcta tcgcgataaa cacgcgatgt ggcggtaaca
    27961 atctaccggt tattttgtaa accgtttgtg tgaaacaggg gtggcttatg ccgcccctta
    28021 ttccatcttg catgtcatta tttcccttct gtatatagat atgctaaatc cttacttccg
    28081 catattctct gagcgggtat gctacctgtt gtatcccaat ttcatacagt taaggacagg
    28141 ccatgagtaa gatttttgaa gataactcgc tgactatcgg tcacacgccg ctggttcgcc
    28201 tgaatcgcat cggtaacgga cgcattctgg cgaaggtgga atctcgtaac cccagcttca
    28261 gcgttaagtg ccgtatcggt gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga
    28321 aaccaggcgt tgaactggtt gaaccgacca gcggtaatac cgggattgca ctggcctatg
    28381 tagctgccgc tcgcggttac aaactcaccc tgaccatgcc agaaaccatg agtattgaac
    28441 gccgcaagct gctgaaagcg ttaggtgcaa acctggtgct gacggaaggt gctaaaggca
    28501 tgaaaggcgc aatccaaaaa gcagaagaaa ttgtcgccag caatccagag aaatacctgc
    28561 tgctgcaaca attcagcaat ccggcaaacc ctgaaattca cgaaaaaacc accggtccgg
    28621 agatctggga agataccgac ggtcaggttg atgtatttat tgctggcgtt gggactggcg
    28681 gtacgctgac tggcgtcagc cgctacatta aaggcaccaa aggcaagacc gatcttatct
    28741 ctgtcgccgt tgagccaacc gattctccag ttatcgccca ggtgctggca ggtgaagaga
    28801 ttaaacctgg cccgcataaa attcagggta ttggcgctgg ttttatcccg gctaacctcg
    28861 atctcaagct ggtcgataaa gtcattggca tcaccaatga agaagccatt tctaccgccc
    28921 gtcgtctaat ggaagaagaa ggtattcttg caggtatctc ttctggagca gctgttgccg
    28981 cagcgttgaa actacaagaa gatgaaagct ttaccaacaa gaatattgtg gttattctac
    29041 catcatcggg tgagcgttat ttaagcaccg cattgtttgc cgatctcttc actgagaaag
    29101 aattgcaaca gtaatgccag cttgttaaaa atgcgtaaaa aagcaccttt ttaggtgctt
    29161 ttttgtggcc tgcttcaaac tttcgcccct cctggcattg attcagcctg tcggaactgg
    29221 tatttaacca gactaattat tttgatgcgc gaaattaatc gttacaggaa aagccaaagc
    29281 tgaatcgatt ttatgatttg gttcaattct tcctttagcg gcataatgtt taatgacgta
    29341 cgaaacgtca gcggtcaaca cccgccagca atggactgta ttgcgctctt cgtgcgtcgc
    29401 gtctgttaaa aactggcgct aacaatacag gctaaagtcg aaccgccagg ctagacttta
    29461 gttccacaac actaaaccta taagttgggg aaatacaatg ttccagcaag aagttaccat
    29521 taccgctccg aacggtctgc acacccgccc tgctgcccag tttgtaaaag aagctaaggg
    29581 cttcacttct gaaattactg tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt
    29641 taaactgcag actctgggcc tgactcaagg taccgttgtg actatctccg cagaaggcga
    29701 agacgagcag aaagcggttg aacatctggt taaactgatg gcggaactcg agtaatttcc
    29761 cgggttcttt taaaaatcag tcacaagtaa ggtagggtta tgatttcagg cattttagca
    29821 tccccgggta tcgctttcgg taaagctctg cttctgaaag aagacgaaat tgtcattgac
    29881 cggaaaaaaa tttctgccga ccaggttgat caggaagttg aacgttttct gagcggtcgt
    29941 gccaaggcat cagcccagct ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa
    30001 gaaaaagaag ccatctttga agggcatatt atgctgctcg aagatgagga gctggagcag
    30061 gaaatcatag ccctgattaa agataaacac atgacagctg acgcagctgc tcatgaagtt
    30121 atcgaaggtc aggcttctgc cctggaagag ctggatgatg aatacctgaa agaacgtgcg
    30181 gctgacgtac gtgatatcgg taagcgcctg ctgcgcaaca tcctgggcct gaagattatc
    30241 gacctgagcg ccattcagga tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa
    30301 accgcacagc tgaacctgaa gaaggtgctg ggtttcatca ccgacgcggg tggccgtact
    30361 tcccacacct ctatcatggc gcgttctctg gaactgcctg ctatcgtggg taccggtagc
    30421 gtcacctctc aggtgaaaaa tgacgactat ctgattctgg atgccgtaaa taatcaggtt
    30481 tacgtcaatc caaccaacga agttattgat aaaatgcgcg ctgttcagga gcaagtggct
    30541 tctgaaaaag cagagcttgc taaactgaaa gatctgccag ctattacgct ggacggtcac
    30601 caggtagaag tatgcgctaa cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac
    30661 ggcgctgaag gcgttggtct gtatcgtact gagttcctgt tcatggaccg cgacgcgctg
    30721 cccactgaag aagaacagtt tgctgcttac aaagcagtgg ctgaagcgtg tggctcgcag
    30781 gcggttatcg ttcgtaccat ggacatcggc ggcgacaaag agctgccata catgaacttc
    30841 ccgaaagaag agaacccgtt cctcggctgg cgcgctatcc gtatcgcgat ggatcgtaaa
    30901 gagatcctgc gcgatcagct ccgcgctatc ctgcgtgcct cggctttcgg taaattgcgc
    30961 attatgttcc cgatgatcat ctctgttgaa gaagtgcgtg cactgcgcaa agagatcgaa
    31021 atctacaaac aggaactgcg cgacgaaggt aaagcgtttg acgagtcaat tgaaatcggc
    31081 gtaatggtgg aaacaccggc tgccgcaaca attgcacgtc atttagccaa agaagttgat
    31141 ttctttagta tcggcaccaa tgatttaacg cagtacactc tggcagttga ccgtggtaat
    31201 gatatgattt cacaccttta ccagccaatg tcaccgtccg tgctgaactt gatcaagcaa
    31261 gttattgatg cttctcatgc tgaaggcaaa tggactggca tgtgtggtga gcttgctggc
    31321 gatgaacgtg ctacacttct gttgctgggg atgggtctgg acgaattctc tatgagcgcc
    31381 atttctatcc cgcgcattaa gaagattatc cgtaacacga acttcgaaga tgcgaaggtg
    31441 ttagcagagc aggctcttgc tcaaccgaca acggacgagt taatgacgct ggttaacaag
    31501 ttcattgaag aaaaaacaat ctgctaatcc acgagatgcg gcccaattta ctgcttagga
    31561 gaagatcatg ggtttgttcg ataaactgaa atctctggtt tccgacgaca aaaaggatac
    31621 cggaactatt gagatcattg ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc
    31681 ggatgtcgtt tttgcggaaa aaatcgttgg tgatggtatt gctatcaaac caacgggtaa
    31741 caaaatggtc gcgcctgtag acggtaccat tggtaaaatc tttgaaacca atcacgcgtt
    31801 ctctatcgaa tctgatagcg gcgttgaact gttcgtccac ttcggtatcg acaccgttga
    31861 actgaaaggc gaaggcttca agcgtattgc tgaagaaggt cagcgcgtga aagttggcga
    31921 tactgtcatt gaatttgatc tgccgctgct ggaagagaaa gccaagtcta ccctgactcc
    31981 ggttgttatc tccaacatgg acgaaatcaa agaactgatc aaactgtccg gtagcgtaac
    32041 cgtgggtgaa accccggtta tccgcatcaa gaagtaattc ttgccgcagt gaaaaatggc
    32101 gcccatcggc gccatttttt atgcttccgc cagcggcggc aaaatcaatt catcgctctc
    32161 atgctgctgg gtgtagcgca tcacttccag tacgcgcaac cccgcacggt gcaccgcatc
    32221 gtttaacgcc ttccctttca gcaaaccact gataagctga gcacaaaaca ggtcgccagt
    32281 ccctttcagg tcggttttta cccgtgaatg ggaaatgaca ttcacgctgt cggcactgac
    32341 caccaccacc tgcatctcct gattttcttc attaccggag gcgctggtaa tcaccaccca
    32401 ttttaatgtg tctgaaagca gactttttgc ggcagcaatg gcactgtcga gatcgcggca
    32461 atttttaccg gtcaggattt ccagctcaaa gatattgggg gtaattccct gcgccagcgg
    32521 cagtaaatat tgtcgatacg cctcgggaag gtcaggtttg acataaattc cgctatcaat
    32581 atcgccaatc accggatcga ccatgatcaa taggtcagga tggtctttgc gtagcgcagt
    32641 cagccactcg gcaaggattt tgatttgcga tgccgttccc atatagcccg tggttacagc
    32701 acgaagttgg cgcagcgcat cacgctcctg aagcgcacgc aaatagccgc taaaccattc
    32761 gtccggaatc gcaccaccgt agaaagtgtc ataatgcggc gtattgctca gcaataccgt
    32821 cggcacggca aagacattca agccgttctg ttttatagca ggcaccgcaa tgctgttgcc
    32881 tacgcttccg tacactacct gcgactgcac ggcgacgata tccgcctgca gcgccctact
    32941 cttatcgtta aacaacaaca aactactcat ttaatttttt ctccttgccg atgatcctca
    33001 tcgtattcca accgaaactt tacctgattc tggcagtcaa atcggctatt acaaaacaaa
    33061 gataaggtaa ttcagtgaag aaaatcattt gtctggccat tacgctgcta atgacactcc
    33121 ccgcttacgc gaagttaact gttcatgaag aagcccgcat caacgccatg ctggagggat
    33181 tagcacagaa aaaggatttg atatttgtgc gcaacggtga tgaacatacc tgcgatgaag
    33241 cggtttccca cctgcgtctg aagctcggca atacccgtaa ccgcattgac actgccgagc
    33301 agtttattga taaggtagct tcgtcgtcat cgattaccgg gaagccgtat atcgtgaaga
    33361 tccccggtaa gagcgatgag aacgcacagc cttttttaca tgcgttaatt gcgcagacgg
    33421 ataaaacggt gcctgcggaa ggaaattaat ccgctttggt aaggcattta caggaggtaa
    33481 catgaaaaaa tgctttattt atcacgatga aaaatcgaat aaattttggt ggatagatta
    33541 cgaaggggat agtttagctg tcaactatgg caaggtaggt agtattggta aattccatac
    33601 aaaagaattc gataatgaag aacagtgtct gaaagaagcc agtaaattga ttgccgcaaa
    33661 aatgaaaaaa ggatatcaag aaactccaga ctttaacttc atggatctct actattttga
    33721 tgatgaagag attgggttac atcttaaaac gtcacaccca aacttccagt gccattttac
    33781 tgatccactt tatatgtgtt gctgggatga agaatctcct tttggtagcg atgaaggtgc
    33841 tgatgctcta aacgaacttg aaaatagcct ccgttctgga ctgtgctgat ttccctcaaa
    33901 tgttaattga aactatgtgg ggtatgaaat acatcgctat ggacagtatt cttgaagagg
    33961 atgtgcgtgc gcaattacta gccgatgaaa tgagctctat ccagagcaat atgattacct
    34021 acgcgactgc attcggtcag atcaaagtca tgggtaaaat ctcccataaa cttaaaaaga
    34081 tgggactcaa tgcactagcg cgtcatcagc ttaccgcaaa aattcttcaa tggggtgacg
    34141 gtcaggactc accaatactt caaaaaatga ttgatgacct tactgctttt ccacacgaaa
    34201 attaaaaact gcatttgtcg gtagcaacaa ctgccgacaa atcagattat tgacaaagtg
    34261 cactttgttt atgccggatg cggcgtaaac gcctgatccg gcataaaaat attgctaatt
    34321 caatatattg cagaaacatt gtaggcctga taagcggagc gcatcaggca gttttgcgtt
    34381 tgtcatcagt ctccaatgct attaatcctt aaatccctgc cccctgacta aaatgctctt
    34441 ccccaaacac tccggtggag aggtagcgat cgccacgatc gcagatgatc gctaccacca
    34501 ccgcaccagg gttagctttt gccacccgca gtgctccagc aaccgcgccg cctgagctga
    34561 cgccacagaa tattccttcc cgcaccgcca gttcgcgcat ggtgttttcc gcatcgcgct
    34621 gatgaatatc cagcacctca tccaccagcg cagcgttgaa aatccccggc agatattccg
    34681 caggccagcg gcgaatgccc ggaatactgc tgccttcttc cggttgcagg ccaacaatgg
    34741 tcaccggttt ggattgttcg cgcataaagc atgaaacgcc ggtgatagtg ccggtcgtcc
    34801 ccatgctgga gacaaaatga gtgatgcgcc cgccggtttg ctgccagatt tccggcccgg
    34861 tggtggtgta atgcgcataa gggttatcgg gattattgaa ctgatcgagc agctttcctt
    34921 cgccacgatt cgccatctcc agcgccagat cgcgcgcacc ttccatgccc tgctctttgg
    34981 tgacaagaat cagttccgca ccataagcac gcatcgccgc acggcgttcc tggctcatgt
    35041 tgtcgggcat cagcaatttc atgcgatagc ctttcagcgc ggcaatcatt gccagcgcaa
    35101 tgccggtgtt accactggtg gcttcgatta agacatcacc cggtttaatt tccccgcgct
    35161 tttccgcctc gacgatcatc gaaagtgccg cacgatcttt caccgaacct gccgggttat
    35221 tgccttccag ttttaaccac acttcactgc cgttatccgg ccccattcgc tgcaacttca
    35281 ccagaggcgt attgcctatt gtttgttcta atgtactcac aatctctatc catacgtggt
    35341 gttgcctgat gcgacgcttg cgcgtcttat caggcctaca ggttacaaac cttgccatta
    35401 aaaagcccgg atcgcgggaa gcgcctccgg gcgtttaaca ttcactcaac ctatcaggcg
    35461 ctttgtgcga gagcaagttc ctcatcgcgg gtttcaatac gctcgtcgcc gttatacagc
    35521 cgcgcatgtt gcagaccaac gaataaacgc tcgccatgct gcggggcatc gtcgccatgc
    35581 atcacgaccg tcagcggttc gttgtaccac cccagcggct gcaccactaa ttgggtgtag
    35641 tgacctttcg ggctggcttc cagtacctgt accggcagcg gcgaatcgag actggtacgg
    35701 cggctgatat ccacttccca cgggcgcagg aagagatcca ccggcccctg atacgcaggt
    35761 gtgtagccca gcggccagcg atgcgcgcca acatggaact gcccgccgcg aatggttccc
    35821 tgcaggcggt tcacttcgcc cataaattcg agtacaaaac gggtcgctgg ttcgcgccat
    35881 acctgattcg gcgcatcagc ctgttcaata ttcccctgac tcatcaccac tacacgatcg
    35941 gctacttcgg tcgcctcttc ctggtcgtgg gtcacaaaca cgctggtgaa tttcagctct
    36001 tcatgcagtt gacgcagcca gcgacgcagc tctttacgca cctgcgcatc cagcgcgccg
    36061 aacggttcat caagcagcag aatttgcggt tctacagcca gcgcgcgcgc cagcgccaca
    36121 cgctgtttct ggccgccgga aagctgcgcc ggataacgat ccgccagatg cgcaagctgg
    36181 accatttcca gcaattttgt cactttcgct ttgatggctg cggcattcgg gcgctcgcga
    36241 cgcggcagca ccgtcaggcc aaaagtgata ttgtcgaata ccgtcatatg gcggaacagc
    36301 gcgtaatgct ggaacacgaa accgacttta cgatcgcgcg cgtgcaggcg actcacgtcg
    36361 gtgccgtgga agcgaatatg cccgctggtt tgatgctcca gcccggcgat aatccgcagc
    36421 agcgtggttt tcccggaacc ggacggcccc agcaacgcga ccatctgacc agaaggaata
    36481 tccagtgaga tatcgttcag cacctgggtg cgaccaaaag acttcttgat attggcaatc
    36541 tcaatgctca tgatgctcct cctgctgcgc gcgtttttcc tgattctcca ggcgccactg
    36601 caacatactc tttaaaaaca gggtgataat cgccatcaac gtcaacagcg cggcggcggt
    36661 aaaggagccg acggtgttgt agtcctgctc caacaattca atctgtaacg gcagcgaaag
    36721 tgtttcgccg cgaatcgagc cggaaaccac cgacactgca ccaaactcgc caatcgcgcg
    36781 ggcgttggtc agcaccacgc cgtaaagcag cgcccagcga atattcggca atgtgacgcg
    36841 acggaacatc tgccagccgg acgcgccaag caaaatcgcc gcttcgtctt cctggctgcc
    36901 ctggcttaac atcaccggca ccagctcgcg caccacaaac ggacacgtca caaagatggt
    36961 gaccagcacc attccgggcc aggagaacat aatttgcaga ttatgttcat ccagccagcc
    37021 gccgagcggg ccgttggagc cgtagaacag cagatacacc agaccggcaa ccaccggcga
    37081 tacagcaaac ggaatgtcca gtagcgtcag cagtaactgg cgtcccggga agttaaagcg
    37141 cgtcaccagc caggccagca gaatgccgaa caccaggttt accggtacgg caatcagcgc
    37201 gatcatcacc gtcagccaga tggcgtgcag catgtccgga tcggccagat tctgtaaaac
    37261 cggcatcagc cccttgctga atgcctgcac gaagatgtaa atcatcggca ccagcaggat
    37321 gaacgccgaa accagcatcc cgatgccaat cagaaaccat ttgccccagt taatcgggcg
    37381 cgcgtcataa cgcttcaatt gggtaacttc cgccattaat gacctaccac acgccgacca
    37441 aagcgacttt gcagagtgtt aattgagaac agcagcagca gagatgccgc gaggatcacc
    37501 gaagcaatcg cgctcgctgc cgggtaatca aactcctgta agcgcacaaa aatcatcagc
    37561 gacgtcactt ccgtcttcca cgcgatattt ccggcgataa aaatcaccgc gccaaattca
    37621 ccaagactac gggtaaacga cagcgccacg cccgccacca gcgccggaga aagctccggc
    37681 agcaccactt tgcagaaact ctgccagcgc gttgcaccaa gcgtttccgc cgcttcttca
    37741 tattccgggc ctaactcttc cagcaccggc tgcacggtac gcaccacaaa cggaatgctg
    37801 gtaaaggcca tcgccaccgc aataccaagc caggtatagg tgactttgat atcaaacttc
    37861 gccagccatt caccgtaaaa accgtttacg gaaaagagcg aggccagcgt taaaccggcg
    37921 actgccgttg gcagcgcaaa gggtaaatcc atcagcgcat caagcagcgt gcggcccgga
    37981 aagcgataac gtgttaaaat ccacgccatc agcagaccga aaacgccgtt aaaaatcgat
    38041 gccacaaacg ccgacagcag cgtcactttg taggccgcga ccacctgcgg gttggtgatc
    38101 acctcccagt actgcgccca gctcatctca gaaagctgca tcaccagcgc agagagcggc
    38161 agcagcaata tcaggcacac aaacagcaga ctggtgccaa ggcttaaagt aaagcccggc
    38221 agcacgcgtc ttgaagaaac tgcaaacatt acttacgccc cgccgctaac agcttgtcta
    38281 actcgccgcc gctggtgaag tgggttttca tcacttccgg ccatgagcca aatttgtctt
    38341 ccacgcggaa cagttcggtc tgcgggaatt tatctttcag tttgtccatg acttccgggt
    38401 tattcacacg gtagtaatag tcggtgatga tggtctgcgc ctgtgggctg tagagccagt
    38461 tcaggtaggc ttttgcggct ttttccgtac cgttggcctg cacattttta tcgacccacg
    38521 ccaccgggaa ttccgccaga atattggttt tcgggatcac cacttcaaag ccctgtgctt
    38581 catactgttt acggatgttg ttcacttccg attcaaagct aatcagcaca tcgcccaagc
    38641 cacgctcggc aaaagtggtg gttgcgccac gaccgccggt atcgaacact tcaacgtttt
    38701 tcaggaactg ggtcataaac tgttcggttt tggctttatc gccaccgtca gctttatccg
    38761 ctgcgcccca tgccgccaga taggtataac gcgcgttacc cgacgttttc gggttcggga
    38821 aaatcagctt cacgtcggag cgcaccaggt cgttccagtc gtggatattc ttcgggttgc
    38881 ccttacgcac caggaagccc atggtggagt agaacggcga gctattattc ggcaggcgcg
    38941 actgccagtc agccgggatc agcttgcctt tgtcgtgcag gatttgtacg tcggtcacct
    39001 ggttataagt gacaacgtct gcttttaagc cctgcaaaat cgccagcgcc tgttttgatg
    39061 acccggcatg agattgtttt atcgtcagtt tgtcgccgcc gttatctttt gcccattgct
    39121 gttcaaacgg cggattcagg gcggcaaaca gctcgcggga gacgtcataa gaactgttca
    39181 gcagttccgt tgcctgtaca tggcccgcca gcagcagaga agcgaccagc gcgagtgagt
    39241 tctttttcag taagttaacg gccattgcgc acccttataa atttaatgac tttctaatag
    39301 ccatcatatt tataacggac gtgaaaggag taacggtttt atataccgtt tggtgatttg
    39361 gaagttaaaa agggaataag aacttggttc aacaggcgta attgttgcag gcagtttgga
    39421 cacggacagc gcggagaaac cggagcgtac acatagtacg tgaggatttc gagcactgcc
    39481 cggggccaaa atgacaaatg aaatagcctg gtgaacttag ttcaagacga aatcctcccc
    39541 acacatgcag ggaggaaaca gaggtgaatc agataccgac gctaaccgtc tccggcagtg
    39601 tgctgccgcc atcaatcaca ttctgtgtac cggttaaata gctggattca tccgatgcga
    39661 ggaaggccgc cagttcgccg acttccagcg gatcggcgag gcgacacagc gggattgctt
    39721 ttgccatttc agtcagcacc gattctggat cttccgggtt cgactggcgg gcaatgcttt
    39781 ccgccattgg cgtgcggacg tatcccgggc agatggcgtt aacgcgaata ccggattgcg
    39841 cgtactccac cgccagcgat ttagtcaggc caacaatcgc cgctttcgtt aaggcatacg
    39901 ccgtttcgcc aggatcagcc accatatcac cagtgactga agacatcatc acaatgcgac
    39961 catctttgcg cgcaatcatc tccggcagca ccgccttcgt gacgttccat acgcctttaa
    40021 tattgatatc aatatggaaa tcgcgatctt catcgctcat atcgaggaaa ctgcccagac
    40081 gacaaacgcc tgcgttattc accaggatat caatgcgccc ttctttttcc ttcgcgcgtt
    40141 tgatagctgc ggctaccgac gccgggtcac gcacatcggc gacaaccgcc gtacagcgat
    40201 gaccacgacc acacagttcg tccgctagct tttcgatctc aggggagata tccagcaaga
    40261 ttaggttcgc gccatgacgt gcaaaagttc tggcaattcc ttcgccaatt ccctgcaatg
    40321 cgcccgtaat cagtgctgtc ttgcccgtga gtttacccat tttaatgctc tccttgttaa
    40381 tacagagcgt tacactccat tccctctgaa aatacaggat aaacaaaata ttgctgcgga
    40441 tcagctcact aattcaggaa aatctcaatc cagtcgctgc tttatgcatc atttcaggcg
    40501 ttgtgttagc tcactgctgc gctgaatcat tttcaatgac gccacatcat tgatgttgca
    40561 caagaccaac aaacagtaaa tcggtaacag aattttgcgc cgtgcgggta gacatcgacg
    40621 agctacgcca ttcggtttca ccggaaacgg tatcgagcgt gaaatgcgcc agccgccgca
    40681 gcggagagtc agccagagaa gtaatggcaa tcaccgttgc cccctgcttt cgtgccgctt
    40741 ctgcgcacaa gacaatctct tttttactgc cgctgtaaga gatagcgatt tgtacatcgc
    40801 cttttttcaa cgcctgggaa acggtggctt gtacgtgcgt gtcggcttcg caggccacgc
    40861 gatagccaat tttcatcagt ttgaacgaca gatcgcgccc caccagcgcc gatccgccca
    40921 ggcccgttat ctggataaac ggtgctttac tgatgacctc aatgattttt tgcagccgcg
    40981 cgtagtcgaa caacgcacag gtttgctcca gcgccagctc tttttcgcga ttcagtttgc
    41041 gggcaatcac ttccagcgaa tcatcactgg taatcgaact gtgcaggtgc agcgccgtgg
    41101 cattcgtttt ctcccggctg gcactgtatt cgccaattaa cgccatacgc aattcagtaa
    41161 atccttgcgc gccgagcttc tgggcaaatt tcacaatgct cgactggctg ataccgagct
    41221 gtttcgccat ctgccgtgaa gagacggatt tcagctcgct cactctggcc cgcagaaagt
    41281 cagcaatttt ttgctcgttc ccggtaaatt cactttccgc attgcgtatt ttcgtcaggt
    41341 acaacatctt ccactctcct aggctggcag caaaaattcc gcttaagttc ttatgacgct
    41401 cttcacactc tgcgagtgta gactcaatga ttcctttaag tctgtcaatt cgggatatat
    41461 gattccatac atctgcaatt atttgatcgt aaatagtaag gtcaccaccg atgcaactag
    41521 aaaagatgat tactgaaggc tcgaacaccg cctcggctga aattgaccgc gtatcgacgc
    41581 tggaaatgtg ccggattatc aacgatgaag ataaaaccgt accgcctgcc gttgatcgcg
    41641 tcctgccgga tatcgccgcg gcgatcgatg ttatccacgc ccaggtcagc ggcggcggtc
    41701 gtctgattta tctcggtgcg ggaacatccg gtcgtctggg gattctggat gccagcgaat
    41761 gtccgcccac ctacggcgtg aaaccgggtc tggtggttgg tttgattgct ggaggcgaat
    41821 atgccattca gcacgcggtg gaaggagctg aagatagccg ggaaggtggt gttaacgatc
    41881 tgaaaaatat taatttaacg gcacaggatg tggtggttgg cattgctgcc agcggtcgca
    41941 cgccgtatgt gattgccgga ctggaatacg cacgccagct cggctgccgc acagtgggaa
    42001 tttcctgtaa tccggggagc gccgtttcaa ccaccgctga gtttgccatt acaccgattg
    42061 taggtgccga agttgttacc ggttcttcgc ggatgaaagc aggtacagcg cagaaactgg
    42121 tgctcaatat gctttccacc gggctgatga ttaaatccgg caaagtgttc ggcaacctga
    42181 tggtcgatgt ggtcgccacc aacgaaaaac tgcatgtgcg ccaggtcaat atcgttaaaa
    42241 acgccaccgg atgtaatgcc gaacaagcgg aagcggcgtt aattgcctgc gagcgcaact
    42301 gtaaaacggc cattgtgatg gtgctgaaaa atctcgatgc cgcagaagct aaaaaacgcc
    42361 tggatcaaca cggcggcttt attcgtcagg ttttagacaa ggaataaccc atggccaaag
    42421 agatcagcag tgaacttctg aacaccattc ttacccgtgt cggcggaccg ggaaatatcg
    42481 ccagttgtgg taactgtatg acgcgcctgc gtctgggtgt acatgacagt tcactggttg
    42541 atcccaatat caaaacgctg gaaggcgtga agggcgtcat tttgaccagc gaccaggtgc
    42601 aggtcgtttt tgggcctggt aaagcgcatc gtgccgcaaa agcgatgagc gagctgctgg
    42661 gcgatgcacc ggtacaggat gccgcagaaa tcgccgccca gaacaaacgt cagttaaaag
    42721 ccaaacaaac ctccggcgtg caacaatttc tcgccaaatt cgccaccatc ttcacgccgc
    42781 tgatccccgg ttttattgcc gccggtctgc tgctggggat agcgacgtta attgccacgg
    42841 tgatgcacgt tccggcagat gctcagggca cgctacccga cgcgctgaat tttatgaagg
    42901 tgttcagcaa aggtttgttc accttcctag tgattctggt gggctataac gccgcccagg
    42961 catttggcgg cacgggcgtc aatggcgcaa ttattgccgc actctttttg ctcggttata
    43021 accccgctgc aaccaccggt tactacgccg gttttcacga tttctttggt ctgcccatcg
    43081 atccgcgcgg caatattatc ggcgtgctga ttgccgcgtg ggcctgcgcc cgcattgaag
    43141 gcatggtgcg ccgctttatg ccggacgatc tcgacatgct gctgacctcg ttaatcaccc
    43201 tgctgatcac cgctacgctt gcgtacctga tcattatgcc gctgggtggc tggttattcg
    43261 aaggtatgtc atggctgttt atgcacctga acagtaatcc gttcggttgt gcggttttag
    43321 ccgggctgtt cctgatcgcc gtggtatttg gcgtgcatca gggctttatt cctgtttacc
    43381 tcgcgttaat ggacagccag ggattcaaca gcttatttcc catcctttca atggcaggcg
    43441 cgggccaggt gggcgcggcg ctggcactct actggcgggc gcaaccgcac agtgcgctac
    43501 gcagtcaggt acgcggggcg attattcccg gcctgctggg cgttggtgaa ccgctgattt
    43561 acggtgtcac cctgccccgc atgaagccgt ttgttaccgc ctgtttaggc ggcgcggcgg
    43621 gcggtttgtt tatcggattg atagcctggt ggggtctgcc gatgggctta aacagcgcct
    43681 ttgggccgtc tggtctggtg gcgctgccgc tgatgacttc cgcacaaggc atccttccgg
    43741 caatggcggt ttatgctggc gggattctgg tggcatgggt ttgcgggttt attttcacca
    43801 cgctctttgg ctgccgtaac gtcaatctgg actgatatta tgaaacggac aatgctctat
    43861 ctttctctgc tggctgttag ttgtagcgtc agcgccgcaa aataccctgt tctgacagaa
    43921 ggtagttgat caagtatatg cggcgttaaa gcagtaataa aaaggccagt cagtaatgtt
    43981 actgcctggc ctttaatcaa tgaatcagaa acgcttacag cgccatcaac ttgtccagcg
    44041 acggtgcgaa ataatagccg ccggttaccg gtttggtgaa acgcaacatc gcatcacgtt
    44101 tgccatcggt atcgccaaac atgctcagca gttgctgctc aatgttatgc agacgcgcgc
    44161 agtaggcgca gaagtacaga ccgtgagtgc cactggcggt gccgtacggc aggctctggc
    44221 gaacaatctt cagccctttg ccatcttctt tcagatcaac gcgggtgagg tgagaggttt
    44281 ccggacgttc gtcgccgtca atctcttcgt tggcctcttt ggtgcgcccg atcatcatct
    44341 cctgatcgtg aacgctcatc cggttgagct gcttcaggtt gtgctcccaa cgctgcacaa
    44401 acacatagct gccgccggaa tccacgccgt ctttgataac cgccacttcg cggcgcgtct
    44461 cttcacccgc cgggttttct gtaccgtcaa caaagccgct caggtcacgc tcttccaccc
    44521 aacggaagcc gtggatctct tctttcactt caatgcagtc accaaaggct tccatcgccg
    44581 cctgagcgac agagaagttt acgtcgtgac gcagagaaag aatgtggatc aacacatcga
    44641 actgggtcgt cggcgcaagg cctttaccgt agcccggaaa atctttcagc tcttctgccc
    44701 caacgccgcc gctcagagcg cgccaggtgt tgttaccaaa ggcaaccacc gcaccaagat
    44761 gcgcgtccgg gaatttcgct tcaaaagttg ccagtttgtc ggcaaatgtt ttactggccg
    44821 cacgcagggc gtcaacttcc cctttcacgt tggcttcgat ccaaatcgcc gcgcggcaat
    44881 gttctggcaa aatgccactc tgaacctgag acattgttcc tcctgaaaat aagaatgcca
    44941 cgcaatccgt ggcattgttg agcgctattt tacgtgtttt ttcgcgacgg cggtttgttc
    45001 agacgcaaat taacgccgcc agataatttt actgactttc cagtttttca gggtatcgtc
    45061 cgaaggcatt aacccttcag gaccacgcca ttccccgctg aactggtagc taatatgttg
    45121 actgccttcg gctttacatt cgacggcacg attatcatca ccatcagctt tttggcaatt
    45181 gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg attttaacac cggtgtcagc
    45241 aggaatatcg ctatccagca cgtcaatgcg actgatcgtg ccttgatcgc cgttaatcac
    45301 catcgccacg ttgtcgcctt tcatcacttc aaaaaagcgc accacgttgc cgttcgtggt
    45361 tttcattccg ctgcgcaggc gataatcgcc atcaagcgca tcagcaatgg cttgttcttg
    45421 cagtggtgtg gacgccgtta attcacccac gccctgctcg ctcactttgg tggacgatcc
    45481 aaaccagttc cacgggttag cggcagacca gttaactgac gacatcgtgg aacagccggt
    45541 cagcatcaac ggcatagcgc ataacattaa acgcagcgat ttcatgtcac ttcctttggt
    45601 tattcaataa cgttgcttgg agtgcaaatt caccaaaaag tgccgttatt ctttttcttc
    45661 attaaaacaa gcgcgtaaac gtcgattggt cagcagccag attaacgcca caatatccgc
    45721 cactaccagc gccagaccga taccgctaac ggattcaccg ttcagccaca gccacggttg
    45781 ccagcaaagt aaaaccacct gcgccaacag caacagaaaa tagagcacat gccaggtgcg
    45841 aggaaacgaa gcccgccgac cgctcaacaa aaacgccagc accgccggaa taccgggaat
    45901 cagccccagc cagaaattat cgtgatcggg ataaaacaga tttagcagcg cagtaccctg
    45961 ctcacgcgac gcaccggcaa tgacaaacag cacccaggtt cgcgcctgaa gcaatagcac
    46021 aagccagaag agcaagggta aacgcaggcg tccgtgcgca tcataatgga caggatggaa
    46081 ctcagtactc ttcatcttca atcaaacgct tacccagact cagcacgtcg gcgtgttcat
    46141 atcccaggcg ttcatacatt ccgagcacca tgtcgttatc ttccggcacg ttgatctgaa
    46201 ttttcgggca gccgcgagca atcagctttt tctccagccg gttaagcaac gcattggcaa
    46261 ttccacgccc acgaaactct ggatgcacgc caagataata agcagacccg cgatgcccgt
    46321 cataaccgcc catcaccgtt ccgaccactt caccgtttac ctcagcgacc aaaaacaaac
    46381 tgacgtcatg gttcatctta cgctcgatgt ccatttccgg atcgttccac ggacgcagca
    46441 agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc ttcctggcga aatacgcgta
    46501 tctccatggt attcgttacc tttttgcggg ttaaaaggct gattatggcg tgaacggtcg
    46561 aattagccaa tatctgacga aaatcggttg aaaaagtggc ataatgggga gttgtcaact
    46621 attgaaatga aaagtaaaac aattctcaac agcaaaccgt cgtaacggat tacgcgatac
    46681 gatataacat ctgaaacttt attattacaa ctcaggccgt atgagcactt ttaaaccact
    46741 aaaaacactc acttcgcgcc gccaggtgct gaaagccggt ttagctgccc tgacgttgtc
    46801 aggaatgtcg caagccattg ccaaagacga acctttaaaa accagcaacg gacacagcaa
    46861 gccgaaagcc aaaaaatctg gcggcaaacg tgtcgttgtt ctcgatccag gccacggcgg
    46921 aattgatacc ggagcgatcg gacgcaacgg ttcgaaagaa aaacatgtgg tgctggcgat
    46981 tgctaaaaac gtccgttcca ttttgcgtaa tcatgggatt gatgcgcgtt taacgcgttc
    47041 tggcgatacg tttatcccac tttacgatcg cgttgaaatc gcccataaac atggcgcaga
    47101 tctgtttatg tcaattcatg ccgatggctt taccaacccg aaagctgccg gtgcttcggt
    47161 atttgccctc tctaaccgtg gggcaagtag cgcaatggcg aaatacctgt ctgaacgcga
    47221 aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac aaggatcacc tattgcagca
    47281 agtactgttt gatctggtgc aaaccgatac tattaaaaac agcctgacgc ttggctcgca
    47341 tattctgaaa aagattaagc cggtgcataa actgcacagc cgcaacaccg aacaagccgc
    47401 gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg gtggaaacct cgtttatcac
    47461 caacccggaa gaagagcggc tgttaggcac ggcggcgttt cgtcagaaaa tcgccacagc
    47521 gattgctgaa ggcgtgatca gttatttcca ctggttcgac aaccagaaag cacattcgaa
    47581 aaagcgataa gttatgaaac ccgacgcaca ccaggttaaa cagtttctgc tcaaccttca
    47641 ggatacgatt tgtcagcagc tgaccgccgt cgatggcgca gaatttgtcg aagatagttg
    47701 gcagcgcgaa gctggcggcg gcgggcgcag tcgggtgttg cgtaatggtg gtgttttcga
    47761 acaggcgggc gtcaactttt ctcacgtcca cggtgaggcg atgcctgctt ccgccaccgc
    47821 tcatcgcccg gaacttgccg ggcgcagttt cgaggcgatg ggcgtttcac tggtagtgca
    47881 tccgcataac ccgtatgttc ccaccagcca cgcgaatgtg cgttttttta ttgccgaaaa
    47941 accgggtgca gagcccgttt ggtggtttgg cggcggcttc gatttaaccc ctttctatgg
    48001 ttttgaagaa gacgccattc actggcaccg caccgcccgt gacctgtgcc agccatttgg
    48061 tgaagacgtt tatccccgtt acaaaaagtg gtgcgacgat tacttctacc tcaaacatcg
    48121 caacgaacag cgcggcattg gcgggctgtt ctttgatgat ctgaacacgc cagatttcga
    48181 ccactgtttt gcctttatgc aggcggtagg caaaggctac accgacgctt atttaccaat
    48241 tgtagagcga cgtaaagcga tggcctacgg cgagcgcgag cgcaattttc agctctaccg
    48301 tcgcggtcgt tatgtcgagt tcaatctggt ctgggatcgc ggcacgctgt ttggcctgca
    48361 aactggcggg cgcaccgagt cgatcctgat gtcaatgccg ccactggtac gctgggagta
    48421 tgattatcag ccaaaagatg gcagcccaga agcggcgtta agtgagttta ttaaggtcag
    48481 ggattgggtg taactccctc acccccattc ccgcatccgc tgatgcaacg tcaacgacgg
    48541 cttctcggca aacagctgct ggtaatccgt ggcaaattgc cccagatgcc agaatcccca
    48601 ctgcatggcg gcgtctttta ccgttgtgct ttgcgaccac gggcttatca gttcgcggcg
    48661 tacgacgttc aggcgaatgc gtttcagcca cgcgtttggg ccaatgccta aaatagcgtg
    48721 aaacgcgttt tgtagcgtgc ggcgactgac atgcagttga ttacacaaat ccagcaccgt
    48781 caccggttcg gacatgtttt ccagcacata ttcacgggcg cgggaaagca accgacggta
    48841 actctgatga ctgatgcttt ccgccgtcac cattggttgc gcttcttcca gcatcgcccc
    48901 catcgccatt agcaaattat cccccagcac ttttcgcact gcaggctgat ggagattttc
    48961 cgggttctcg caaaacgtcg ccagcgcctg ttggacaaag ccccacagcg cggctttatg
    49021 ctgctctttc acttccagcg ccgactggct acgcagcata tgtaataccc gatccggatt
    49081 gtgcaaaaag ttagcctgac aggtgatgac atcttcagac agcactacgc ccaggatcgt
    49141 ataatcgtcc ggcgtactta attcaaactc ggttccaccg gggcgcgtcg ctatttctgc
    49201 gcttcccaga cattgcgaac cgataaatcc ctgctcaccg cgcgtcgccg gaatgccaaa
    49261 ccagaacgag ttcggccaga ccaggcacga ctgacgcagc gccagaccgg tgtattcacg
    49321 aaaaacctga atatcatcga gtagaatttc cgtaaattca ccatgaaact tgcccggatg
    49381 cagctgatcg taaatctgct gctaggcggt aatcgtcaaa gcatgttcat agacatccgt
    49441 tgtctgtcgt tgatgaacat tatccacttc gaccttcggc gtgagcttca ggttttcggg
    49501 taagggttca tgataaagat ggtgcaaatt ggctgtacgg gtctttttca tgatgttaat
    49561 gccgggtgtt gtaggacacc cggcacctcc gacaggttaa tggggcttga gacgataacg
    49621 actactgcgt ttacgtaacg tcccggcaga aaagagctgt tcgagcgcat ttctggcttt
    49681 ttccagcggc cagccaaagt gggcggccac ttctcctgcc gtcattccct gacatactga
    49741 tgttaacagc gccaacaact cgtcagcaga ttccgcgacg ataactggcg cgtcgggttc
    49801 ctttacgggt tgcttcggct ggcggttaaa gccagtaacc agccactggg tgtcatcgtc
    49861 tggccgaccg atctccttac ggctgatgac acgcccggta cgcatcgcgg cagcacaacc
    49921 tgcgtccagc gccgcacgac acgccgccag atcgccttcc accaccagcg ttaagcgacc
    49981 agggtcaagc acttcgtgac tgagcagacg aacgttagct gctttgagca tggcatccgc
    50041 tgcatctatc gcggcgacca tgccgtccac ttccagcaat cccagtgcat tgatcatcgg
    50101 caacctccgt tacgcacgct ggattggatt acgcgcgatt tccagcactg catcggtaaa
    50161 ggcgttacag gctgctttac acgcggcctg gctaccggtt aaaaatgctg ccgagtagtt
    50221 ggtttcagac ggtggcggga cgtaggtcac cagctgtacg tcggcagatt tcaacgcggc
    50281 atcaatgccg taggtcgctt ccagcggcgg tgccaccaga tacgccatcg gatcgccaag
    50341 cgtgatcccg gcggttgatg aaagataaga accggtacgc gaaaccacat gcgccaggaa
    50401 ggcggtgttt tctgcgtcat tggcccactg gaaagctgcg ccattttcaa tatgtgcaac
    50461 catcgcatcc agaccggcac gcacttccgc cgggtttggc ccaccaagca taatcagcac
    50521 ttcaccggca gtcggtgacg ggccgtgtgc cgctcccgca tacagcgagc gaccatatac
    50581 cacttcgacc atcgcttgtt tggtcgcttc gtcagccgca atgtaggtta cgtcgtcaga
    50641 atcggcagaa atcagcccga ggctacgaat atgcggcggc aattttagtt cacgcgcaaa
    50701 ttcagcgtta acggaggcaa tcacccgcat ggcggttacc gacggtcgaa tcaaatctaa
    50761 agctggcatg atgcctcctt atcgggtcat gttgatgccg gatgctttct gctccagcat
    50821 acgtttcgcc aaatccacaa tgacggcaca gcagcacgaa agagaagcaa aagtcagcta
    50881 cctggtacac taggcagcgg gtcagcaaag gggggcaaat gccccttttt tcatgtcaga
    50941 taatcagatc attacctcaa taaactaata atcacatcgc ggttacaacc agtttgatag
    51001 tgatagctca aacccagacg tggcgctggc tgcaagcaaa ataagccgca aaaatgcgac
    51061 cccatgttta ctagccgcaa aaatgcggtc ctatttgcca gaacctttta ttattcaatt
    51121 ggttagtgca agaaaaagcg tgattactgt tcaagaagta tccagaaagg tcatgatttt
    51181 gacgctgaaa aaaaataggt gggcattttt gcccactcac tagccgcaaa tttgcccact
    51241 cactagccgc atttttgcgg cccaaagtaa taaatacaga gaaataataa atacagatca
    51301 gcgccaagat ttttcgtctt ttcggcggaa tcatgagact agtgcttacg tcctgataac
    51361 ctccagagca gaccttaact tctgtgcagc aggactacgc tcattgccgc tcggctaggt
    51421 caaatcctca cgataaagct gttcggattg tgaccataat gttaaaggac tctttaaatc
    51481 attccttgac gaattaacta gctggcttaa gcggaatagc atgcaggata aaaacaaaac
    51541 cagcttagca gtggggagag cttcgcttcg ctcgtctcct ggggccaatg ctcaatgaaa
    51601 cgacgtaagg cacggagcgc tcaccatagg tagacacata aacatctgag agcgcacctt
    51661 ctatcgcgct acgcgctcaa taaaaaccaa cgacaccgcc acactgcaag accaatggtt
    51721 tttatcgcgt atacagacca gacaggaaac agcagcaaag cagcgtatag ccaggtgcag
    51781 acagcacact cacacgacgc acagcaggct taagtgatca ctcaggaaac tttatccccg
    51841 ccgccgatca ccgcgaacta tcatgaccgc gccaaattcg aaaggaatgg cccatagaac
    51901 gcgatgaaca cacataagcg ctacacttac ttaacgtcgc cacagagcga ttgagagcgc
    51961 ttaaaatcga tgcgatttca ttaaattttt ctcactcgcc tgaatggtgg cgatcaggac
    52021 tgatcgcaac attgccccgc ctcgatattg agcgcataat agatacatac attaagcgga
    52081 aaagagataa gtttattgtg aaaatcaggg ggttacatat catgcgcata tcagaacaac
    52141 aacggcagat cctcgactat ctggcaaaaa acgaatcaat caccgcacga caagccgcag
    52201 atctcgttta tggtggcaat gttacccgcg tccaggttga cgccgcccgc cgctcactca
    52261 tgacgatggt tagtaatggg ctaatcagga aacaaggccg cgctttcgta gctgaaagac
    52321 atatcaatca cgcttatagc caggacgttg aagaattgcg ccgccagatc ctttgggtat
    52381 tcagcaaaca gggtcaggag tatttccttt ctgaacaccc aaatttcgcc gccaaagagt
    52441 taacagtaag cggattgtcg tgcgcactgg attatcttgt cggtgaaggt tgcgttactt
    52501 acaagcagct atggcacgcc ctgaatacgc ttgtagaaga tggtttactc attgccacgc
    52561 gtgagaacat cgaatactgg ataatgccgc gttcaggtcg ccgcaatcat gcacggtata
    52621 tttcaggcaa cagatacaga acgccggact ttaatccgac acaggaagat cgggaccgtg
    52681 ccgaaatgat gtgcagggaa caggagaaaa gaattatgga taagttattt tcgatgcagt
    52741 gaacacaagc gggcgcaatg cccgttttta ttgccttatt ccacgcctga cgaatcaatt
    52801 tgatcacacc tgagaaaatg catagccaga agttaaggcc gttatattgc gattgggagc
    52861 gttttaaacg gtatcaattt aattaattct gatgtggtga tttgcaggca aaaaaaaccc
    52921 gccggagaag cgggaaatag agacatatcg ttgtagttat taacagatca ctttgaggtc
    52981 gagggcggct aaccctcgct aatattatcc cgctattacc ttgcgggtgc aaataatctt
    53041 ttattgcttt gatgatttaa taaatagcac tttttgctaa caaaaaccgc tgtcaatata
    53101 tggccaaaaa tgtcaacaga aaaaataagg tttataatat taagtatcac atgggtaatg
    53161 ggttcaggga atcggaatcc accgcctcac ggaaattcaa aatttgcttc ttctttcctc
    53221 cgtgggcggc ttctttttcg tatcgaggta tttatgaaag caataacatt agaacagttc
    53281 gcagcaatgg cgaatgtatc gaccagaacg ctgaggcgct ggctaaaaaa accatcaacc
    53341 ggagtccctt tgccatttac cccacgcggt actcgccaat tattctggga gcacgaatgc
    53401 aaagaatggt tgtacagaac agaacgataa taatcacgca tcacattttg aggtaaacat
    53461 catgaccaat atcgaaagat tagaacgctg tgccgatatt atgcgacgca gatggattta
    53521 tgaccctgaa gcgggtttat tgtttagccg tgaaacaaaa gacgttatca aaggcagcat
    53581 gaccaataaa gggcatttgg ttgtaacggt acatgaaggt aaattcaaag tgacactcac
    53641 ctatcagaaa gcctgctatg tatacacata cggcgcttat gacgagacat tatatgaagt
    53701 gcaccacgtt aatttgaaca aacaggataa ccgcatcaag aatattcgcc tgatgccgaa
    53761 agaaaagcac cgccggatac ataaaaacgt ccgcaaactt attcggctgg gccacacaca
    53821 atggcttcaa ccgcaattaa ttgaacaatc actataaaca caggagtaaa taccatgcat
    53881 tcattagaca gttattttca gcgcacgaca gcccctaaat cagccgccca ggaacgccgt
    53941 gaggaattcc atgagaaagt gatgcgttct gctgattata ttgcggacaa gttcgtggaa
    54001 acggttcgtc cacttgttga tgaagtagct gataaacttc agtctgaaat gccggaagat
    54061 atggaaggca ctgcaaagcg gcgactcatt tgcgaattaa gtcgtcgttt tggcgtgtca
    54121 ataagcgctt ttaaataatc gaaaaaaggg gggaaagata atggacgcat ttgaggtact
    54181 ggcaaataaa ggcgctgaac tggtagcaca gcgtgataaa accgccaacg aaggggaacg
    54241 cgcactgcta aataaacaga ttaaagcgat ccgtatggcc cagtttaaat taatcagtaa
    54301 tgaagttatc gaaacaatta aaccgcagat cccacaaatc gttgctgacg ctattaaagc
    54361 cgccggactg gagaaacgca ttgccgggct aaaaacgaac gcagagaaaa tggagatgta
    54421 caaacagagc ggtgctccga atcccgatga gtacttctca ccggatgttg agtttatggc
    54481 gcaggttgag gaacgtctgg gtagctgtct caccgaggaa cagcgtcgct attttgatgg
    54541 agtggatagt tcggcaggta ttgacctgaa ctcgtatttc ggtcgtgaaa ttgagcattt
    54601 tgatgccgcc ccgtctatgc cggagccaga accagaaatg gccaccgatc ctgaagctga
    54661 gcaacggaaa cgcgtattca atgcgatagg gattcggtac tgatcggata aaagaaagcc
    54721 gggaattacc ccggctgact taacaatgtg accaatgaac aaatcggaca agacaaacag
    54781 gataagcagt ctcgaaacaa tgttcgggaa ctatattagc acaagtccgg agaaaatcat
    54841 catgttggca ctacgcatca tgcagggcat cgctaagacc ctcgcagaac atgtcttaga
    54901 cctgaaacat tcgccactga gcaaacaggc catgaagcgc caaacgcttc gtctgtgggc
    54961 tgaatattcg cttggaacta tcaataaaat catcgacatg aaatcggggc cgagcaacca
    55021 gagcgcggag gaaatggagt tcatcaggcg tttaattctc attcgcagag acattcatag
    55081 ccagttacac agcgtgggaa tcgatattaa tgatgggacg ggcgattaat tttcggacaa
    55141 gtactatgcg cgcgcgagga gctgaacatg aagcaagata ttgcagacag gctggagata
    55201 ctcgaaggcc agcgggcaga agcgaaacaa ctacgtaagc aggccagaag agcgcacagg
    55261 aacaacgaag cagagttact aaccaaatac atcagtttca ccaattactg catctacgag
    55321 tgttacaagg aagatgcaga ggactggctg gattcattgc cagaacagta ctgagcagaa
    55381 taacaccacg tcatgttgtt ctgaggtgag catatcacct ctgttcaggt ggccaaattt
    55441 agtgtgccac ttcagcatca ccgttgaaat cacaataacg atttttttgt aaatatgctc
    55501 tcacatatgt aggaatagac caggttggca ttgaaacaca tctaccat
//
LOCUS       DAZPEU010000005        55418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPEU010000005 DAZPEU010000000
VERSION     DAZPEU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.005"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHU2L_000229"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHU2L_000229"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR1355250.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHU2L_000230"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHU2L_000230"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR1355251.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHU2L_000231"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHU2L_000231"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR1355252.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACHU2L_000232"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACHU2L_000232"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR1355253.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACHU2L_000233"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACHU2L_000233"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR1355254.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACHU2L_000234"
     CDS             7826..9559
                     /locus_tag="ACHU2L_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR1355255.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACHU2L_000235"
     CDS             9708..10559
                     /locus_tag="ACHU2L_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1355256.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACHU2L_000236"
     CDS             complement(10546..10887)
                     /locus_tag="ACHU2L_000236"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1355257.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACHU2L_000237"
     CDS             complement(10889..11767)
                     /locus_tag="ACHU2L_000237"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR1355258.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACHU2L_000238"
     CDS             complement(11733..14030)
                     /locus_tag="ACHU2L_000238"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1355259.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACHU2L_000239"
     CDS             complement(14081..14401)
                     /locus_tag="ACHU2L_000239"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1355260.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACHU2L_000240"
     CDS             complement(14416..15495)
                     /locus_tag="ACHU2L_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR1355261.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHU2L_000241"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHU2L_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACHU2L_000242"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACHU2L_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="ACHU2L_000243"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="ACHU2L_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="ACHU2L_000244"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="ACHU2L_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHU2L_000245"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHU2L_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR1355262.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHU2L_000246"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHU2L_000246"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR1355263.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHU2L_000247"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHU2L_000247"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR1355264.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25474..25608
                     /locus_tag="ACHU2L_000248"
     CDS             25474..25608
                     /locus_tag="ACHU2L_000248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355265.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHU2L_000249"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHU2L_000249"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR1355266.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHU2L_000250"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHU2L_000250"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR1355267.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="ACHU2L_000251"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="ACHU2L_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR1355268.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="ACHU2L_000252"
     CDS             30120..30728
                     /locus_tag="ACHU2L_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR1355269.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHU2L_000253"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHU2L_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR1355270.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="ACHU2L_000254"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="ACHU2L_000254"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR1355271.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="ACHU2L_000255"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="ACHU2L_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR1355272.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHU2L_000256"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHU2L_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR1355273.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="ACHU2L_000257"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="ACHU2L_000257"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR1355274.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="ACHU2L_000258"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="ACHU2L_000258"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR1355275.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="ACHU2L_000259"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="ACHU2L_000259"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR1355276.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="ACHU2L_000260"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="ACHU2L_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR1355277.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHU2L_000261"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHU2L_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR1355278.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="ACHU2L_000262"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="ACHU2L_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="ACHU2L_000263"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="ACHU2L_000263"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR1355279.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="ACHU2L_000264"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="ACHU2L_000264"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1355280.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="ACHU2L_000265"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="ACHU2L_000265"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR1355281.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHU2L_000266"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHU2L_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR1355282.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="ACHU2L_000267"
     CDS             complement(44843..45142)
                     /locus_tag="ACHU2L_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR1355283.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="ACHU2L_000268"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="ACHU2L_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="ACHU2L_000269"
     CDS             45895..46494
                     /locus_tag="ACHU2L_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR1355284.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHU2L_000270"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHU2L_000270"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR1355285.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHU2L_000271"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHU2L_000271"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR1355286.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHU2L_000272"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHU2L_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR1355287.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHU2L_000273"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHU2L_000273"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR1355288.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHU2L_000274"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHU2L_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR1355289.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHU2L_000275"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHU2L_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR1355290.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHU2L_000276"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHU2L_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR1355291.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHU2L_000277"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHU2L_000277"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR1355292.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHU2L_000278"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHU2L_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR1355293.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13493 a        15017 c        14164 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAZPEU010000006        51562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPEU010000006 DAZPEU010000000
VERSION     DAZPEU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.006"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(351..893)
                     /gene="ycfP"
                     /locus_tag="ACHU2L_000279"
     CDS             complement(351..893)
                     /gene="ycfP"
                     /locus_tag="ACHU2L_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR1355294.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(916..1941)
                     /gene="nagZ"
                     /locus_tag="ACHU2L_000280"
     CDS             complement(916..1941)
                     /gene="nagZ"
                     /locus_tag="ACHU2L_000280"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR1355295.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(1952..2776)
                     /gene="thiK"
                     /locus_tag="ACHU2L_000281"
     CDS             complement(1952..2776)
                     /gene="thiK"
                     /locus_tag="ACHU2L_000281"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR1355296.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2757..3398)
                     /gene="lpoB"
                     /locus_tag="ACHU2L_000282"
     CDS             complement(2757..3398)
                     /gene="lpoB"
                     /locus_tag="ACHU2L_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR1355297.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3413..3790)
                     /locus_tag="ACHU2L_000283"
     CDS             complement(3413..3790)
                     /locus_tag="ACHU2L_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR1355298.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3793..4152)
                     /gene="hinT"
                     /locus_tag="ACHU2L_000284"
     CDS             complement(3793..4152)
                     /gene="hinT"
                     /locus_tag="ACHU2L_000284"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR1355299.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4485..6670
                     /gene="fhuE"
                     /locus_tag="ACHU2L_000285"
                     /pseudo
     CDS             4485..6670
                     /gene="fhuE"
                     /locus_tag="ACHU2L_000285"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(6730..8163)
                     /gene="ptsG"
                     /locus_tag="ACHU2L_000286"
     CDS             complement(6730..8163)
                     /gene="ptsG"
                     /locus_tag="ACHU2L_000286"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR1355300.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8458..9255)
                     /locus_tag="ACHU2L_000287"
     CDS             complement(8458..9255)
                     /locus_tag="ACHU2L_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1355301.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9266..10270)
                     /gene="holB"
                     /locus_tag="ACHU2L_000288"
     CDS             complement(9266..10270)
                     /gene="holB"
                     /locus_tag="ACHU2L_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR1355302.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10267..10908)
                     /gene="tmk"
                     /locus_tag="ACHU2L_000289"
     CDS             complement(10267..10908)
                     /gene="tmk"
                     /locus_tag="ACHU2L_000289"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1355303.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10898..11920)
                     /gene="yceG"
                     /locus_tag="ACHU2L_000290"
     CDS             complement(10898..11920)
                     /gene="yceG"
                     /locus_tag="ACHU2L_000290"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR1355304.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11923..12732)
                     /gene="pabC"
                     /locus_tag="ACHU2L_000291"
     CDS             complement(11923..12732)
                     /gene="pabC"
                     /locus_tag="ACHU2L_000291"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR1355305.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(12852..14093)
                     /gene="fabF"
                     /locus_tag="ACHU2L_000292"
     CDS             complement(12852..14093)
                     /gene="fabF"
                     /locus_tag="ACHU2L_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR1355306.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14180..14416)
                     /gene="acpP"
                     /locus_tag="ACHU2L_000293"
     CDS             complement(14180..14416)
                     /gene="acpP"
                     /locus_tag="ACHU2L_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1355307.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14627..15361)
                     /gene="fabG"
                     /locus_tag="ACHU2L_000294"
     CDS             complement(14627..15361)
                     /gene="fabG"
                     /locus_tag="ACHU2L_000294"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR1355308.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15374..16303)
                     /gene="fabD"
                     /locus_tag="ACHU2L_000295"
     CDS             complement(15374..16303)
                     /gene="fabD"
                     /locus_tag="ACHU2L_000295"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR1355309.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16319..17272)
                     /gene="fabH"
                     /locus_tag="ACHU2L_000296"
     CDS             complement(16319..17272)
                     /gene="fabH"
                     /locus_tag="ACHU2L_000296"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR1355310.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17340..18410)
                     /gene="plsX"
                     /locus_tag="ACHU2L_000297"
     CDS             complement(17340..18410)
                     /gene="plsX"
                     /locus_tag="ACHU2L_000297"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR1355311.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18491..18664)
                     /gene="rpmF"
                     /locus_tag="ACHU2L_000298"
     CDS             complement(18491..18664)
                     /gene="rpmF"
                     /locus_tag="ACHU2L_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR1355312.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18716..19237)
                     /gene="yceD"
                     /locus_tag="ACHU2L_000299"
     CDS             complement(18716..19237)
                     /gene="yceD"
                     /locus_tag="ACHU2L_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR1355313.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            19436..20020
                     /gene="yceF"
                     /locus_tag="ACHU2L_000300"
     CDS             19436..20020
                     /gene="yceF"
                     /locus_tag="ACHU2L_000300"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR1355314.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20132..21091)
                     /gene="rluC"
                     /locus_tag="ACHU2L_000301"
     CDS             complement(20132..21091)
                     /gene="rluC"
                     /locus_tag="ACHU2L_000301"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR1355315.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21664..24855
                     /gene="rne"
                     /locus_tag="ACHU2L_000302"
     CDS             21664..24855
                     /gene="rne"
                     /locus_tag="ACHU2L_000302"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR1355316.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25052..25994)
                     /gene="flgL"
                     /locus_tag="ACHU2L_000303"
                     /pseudo
     CDS             complement(25052..25994)
                     /gene="flgL"
                     /locus_tag="ACHU2L_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(26006..27648)
                     /gene="flgK"
                     /locus_tag="ACHU2L_000304"
                     /pseudo
     CDS             complement(26006..27648)
                     /gene="flgK"
                     /locus_tag="ACHU2L_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(27714..28655)
                     /gene="flgJ"
                     /locus_tag="ACHU2L_000305"
     CDS             complement(27714..28655)
                     /gene="flgJ"
                     /locus_tag="ACHU2L_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR1355317.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28655..29752)
                     /gene="flgI"
                     /locus_tag="ACHU2L_000306"
     CDS             complement(28655..29752)
                     /gene="flgI"
                     /locus_tag="ACHU2L_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR1355318.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29764..30462)
                     /gene="flgH"
                     /locus_tag="ACHU2L_000307"
     CDS             complement(29764..30462)
                     /gene="flgH"
                     /locus_tag="ACHU2L_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR1355319.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30515..31297)
                     /gene="flgG"
                     /locus_tag="ACHU2L_000308"
     CDS             complement(30515..31297)
                     /gene="flgG"
                     /locus_tag="ACHU2L_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR1355320.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31435..32022)
                     /gene="flgF"
                     /locus_tag="ACHU2L_000309"
                     /pseudo
     CDS             complement(31435..32022)
                     /gene="flgF"
                     /locus_tag="ACHU2L_000309"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(32124..32186)
                     /locus_tag="ACHU2L_000310"
                     /pseudo
     CDS             complement(32124..32186)
                     /locus_tag="ACHU2L_000310"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32206..33345)
                     /locus_tag="ACHU2L_000311"
     CDS             complement(32206..33345)
                     /locus_tag="ACHU2L_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR1355321.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(33370..34065)
                     /gene="flgD"
                     /locus_tag="ACHU2L_000312"
     CDS             complement(33370..34065)
                     /gene="flgD"
                     /locus_tag="ACHU2L_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR1355322.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34077..34480)
                     /gene="flgC"
                     /locus_tag="ACHU2L_000313"
                     /pseudo
     CDS             complement(34077..34480)
                     /gene="flgC"
                     /locus_tag="ACHU2L_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(34484..34900)
                     /gene="flgB"
                     /locus_tag="ACHU2L_000314"
     CDS             complement(34484..34900)
                     /gene="flgB"
                     /locus_tag="ACHU2L_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR1355323.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35055..35714
                     /gene="flgA"
                     /locus_tag="ACHU2L_000315"
     CDS             35055..35714
                     /gene="flgA"
                     /locus_tag="ACHU2L_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR1355324.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            35790..36083
                     /gene="flgM"
                     /locus_tag="ACHU2L_000316"
     CDS             35790..36083
                     /gene="flgM"
                     /locus_tag="ACHU2L_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR1355325.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36088..36504
                     /gene="flgN"
                     /locus_tag="ACHU2L_000317"
     CDS             36088..36504
                     /gene="flgN"
                     /locus_tag="ACHU2L_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR1355326.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(36544..38079)
                     /gene="murJ"
                     /locus_tag="ACHU2L_000318"
     CDS             complement(36544..38079)
                     /gene="murJ"
                     /locus_tag="ACHU2L_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR1355327.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38246..39112)
                     /locus_tag="ACHU2L_000319"
                     /pseudo
     CDS             complement(38246..39112)
                     /locus_tag="ACHU2L_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(39114..39761)
                     /locus_tag="ACHU2L_000320"
     CDS             complement(39114..39761)
                     /locus_tag="ACHU2L_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR1355328.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39772..40356)
                     /gene="rimJ"
                     /locus_tag="ACHU2L_000321"
     CDS             complement(39772..40356)
                     /gene="rimJ"
                     /locus_tag="ACHU2L_000321"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1355329.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40592..41800
                     /gene="mdtH"
                     /locus_tag="ACHU2L_000322"
     CDS             40592..41800
                     /gene="mdtH"
                     /locus_tag="ACHU2L_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR1355330.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41864..42511
                     /gene="grxB"
                     /locus_tag="ACHU2L_000323"
     CDS             41864..42511
                     /gene="grxB"
                     /locus_tag="ACHU2L_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR1355331.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            42645..43205
                     /locus_tag="ACHU2L_000324"
     CDS             42645..43205
                     /locus_tag="ACHU2L_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1355332.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43311..44357
                     /gene="pyrC"
                     /locus_tag="ACHU2L_000325"
     CDS             43311..44357
                     /gene="pyrC"
                     /locus_tag="ACHU2L_000325"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR1355333.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44431..44676
                     /gene="dinI"
                     /locus_tag="ACHU2L_000326"
     CDS             44431..44676
                     /gene="dinI"
                     /locus_tag="ACHU2L_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR1355334.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            44966..45220
                     /gene="bssS"
                     /locus_tag="ACHU2L_000327"
     CDS             44966..45220
                     /gene="bssS"
                     /locus_tag="ACHU2L_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR1355335.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45335..46453
                     /gene="solA"
                     /locus_tag="ACHU2L_000328"
     CDS             45335..46453
                     /gene="solA"
                     /locus_tag="ACHU2L_000328"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR1355336.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            46501..46614
                     /locus_tag="ACHU2L_000329"
     CDS             46501..46614
                     /locus_tag="ACHU2L_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR1355337.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            46875..47441
                     /locus_tag="ACHU2L_000330"
     CDS             46875..47441
                     /locus_tag="ACHU2L_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR1355338.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            47445..48020
                     /locus_tag="ACHU2L_000331"
     CDS             47445..48020
                     /locus_tag="ACHU2L_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR1355339.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(48062..49114)
                     /locus_tag="ACHU2L_000332"
     CDS             complement(48062..49114)
                     /locus_tag="ACHU2L_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR1355340.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            49339..50259
                     /gene="lpxL"
                     /locus_tag="ACHU2L_000333"
     CDS             49339..50259
                     /gene="lpxL"
                     /locus_tag="ACHU2L_000333"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR1355341.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            50431..50616
                     /locus_tag="ACHU2L_000334"
                     /pseudo
     CDS             50431..50616
                     /locus_tag="ACHU2L_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            50652..>51562
                     /locus_tag="ACHU2L_000335"
     CDS             50652..>51562
                     /locus_tag="ACHU2L_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1355342.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        12602 a        13710 c        12731 g        12519 t
ORIGIN      
        1 taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata acatgaatgg
       61 tgcattggtc ataccaaaat tgatgttaat caagttttgt tgcttaaaag ataggcaact
      121 ttaccggggg agatcaaaaa atagagttta ttgtttatta tttgagcaaa ttcggaaggg
      181 taatataggg tgaagcgtat atcgagggcg tttggtcgtc ctgataagat gcgcagcatc
      241 gcatcaggca ttgatgctcg actgtcggat gcggcgtgga cgccttatcc gacttacaga
      301 attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg
      361 gttttgaacg ctttaatgcg ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc
      421 tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg ctggctgtta
      481 agtgcttcat cattacgcga caaaataacc agacaacgat cgcgattctt ctcgcggaag
      541 ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc
      601 atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa
      661 ccaatccgtt ccgcccagta tccgcccagg ccaacgccgc aaattagcgg acgctcgtca
      721 acgttcagtt gcaacatttt gtccacttct ttaagcagat gctgcatatc atgtttcgga
      781 tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact
      841 ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc
      901 tcacataagc cagggttagt gacctacttt ctcttcctgc cagcgttcat gtaactgatt
      961 cagacgggtg ctgatcgctt tccagcgagc cgagtccatc agttcctgtc gcgaaaatga
     1021 acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac
     1081 gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacccg catccagtga
     1141 tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc
     1201 agagaaaatc acgccgtcaa accccagttc ctgacgcaaa acggttttca gccagtaggg
     1261 agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc
     1321 gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc
     1381 cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg
     1441 tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg
     1501 gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc
     1561 gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat
     1621 ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcccaa
     1681 cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac
     1741 gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg cgcggatctg
     1801 gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc
     1861 tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt aaccttcgac
     1921 atccaacatt actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg
     1981 tgtcatcggc cagcctgata aattgttgat cgccggtttg tcgccagcgg tactcaaacc
     2041 accctccttt gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg
     2101 ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat
     2161 tttccaccca caccgccgcc agttccagcg cgatatcacc atctccagca tactcccagt
     2221 cgatgagttt taaccctgat gcgctatgca ctaaatttcc ggcgtggaca tccatatgca
     2281 atggacttaa gcgtaaaggt cgtggttccc gcgctttgcg cagacgtttt aacattcgca
     2341 gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaagggca
     2401 acagcgttat tcgccagcca aaacgtggtt gttgatgtag ataatacagc aagcctgcca
     2461 gttcgttggt atccggcaaa tacgttttta ccgcaccggg cagatagtcg actaccatcc
     2521 agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc
     2581 gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa
     2641 aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta ttaaacgtcg
     2701 tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat
     2761 tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc
     2821 tgcatttgta gggtcggagc gttaacgttg ccagaggcgc aggagtacag cacgtaatga
     2881 gcgccgacat tgcgggcaat gcctatggct ttactacggg tgcctaaact gtcctgcggc
     2941 gacaaaccta actgttgctt agccatcgac agttgctggg cggaaaccag ggtaaatttc
     3001 ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg
     3061 ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc
     3121 ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc
     3181 ggcgcaggcg gtgccgttcg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc
     3241 ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt
     3301 ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc
     3361 agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc ctttacaaat
     3421 atagataaag tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg
     3481 ccgaatgtgc gggaatggta acgctgcgtg gcctttccag aggatgcatc tccagccctc
     3541 tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa
     3601 tcgttgagga tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga
     3661 tgcccgcagc caataaagat gactccatca ccagcgattg ctcatcattc accggaattt
     3721 ccggatgtga acgacagccc accagcagta acaacaccag agacaccagc ccaaagcatc
     3781 cttttctcat cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa
     3841 caagtgcata tggatgtggt aaacctcttg tccgccatgg cggttggtat tcatgatcag
     3901 acgatagcca tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat
     3961 gcgtcccagc gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgagaatatt
     4021 cggaatgatc agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag
     4081 atcatcctgg tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt
     4141 ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc
     4201 gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaatc ttaactcacg
     4261 atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc
     4321 tgcgaatgcg ctgaaaagac attctctgtt tggtttacac gacaaatatg aatgcgtata
     4381 tttctcattt gcatttgcag caaaattatt cgcacactaa atataaccgc tcatcaccct
     4441 cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat
     4501 ttaacaggga taatcaatat caagtcatca ccaaaccgtc actacttgcc ggttgcatag
     4561 cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa acggtgattg
     4621 ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct
     4681 ctgcgggtac caaaatgcag atgactcaac atgatattcc tcagtcggtc actattgtta
     4741 gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc
     4801 tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc cgcggattcc
     4861 agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg
     4921 gcgacgcact ttctgatatg gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag
     4981 gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga
     5041 cctgtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt
     5101 atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg
     5161 gcggctacca gaataacgac tcatggctgg accgctacaa tagtgaaaag accttcttct
     5221 cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc
     5281 agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca
     5341 gcagcaacag ttacgatcgc gcacgcagta ccgcacctga ctgggcgtac aacgataaag
     5401 agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacac
     5461 tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg
     5521 taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatc
     5581 atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg
     5641 ctgacggtag ttatgaattg tttggtcgtc agcacaatct aatgtttggt ggcagttaca
     5701 gcaaacaaaa caatcgttca gttcatgggc caacatcttc ccggatgaaa ttggcagttt
     5761 ctacaacttt aatggcaatt tcccacaagc cgactggtca tcacagagcc tggcgcagga
     5821 cgataccaca catatgaaat cgttatatgc tgccactcgt gtcacccttg ccgatccgct
     5881 gcatctgatc ctcggcgcac gttataccaa ctggcgggtt gatacgctga cttacagcat
     5941 ggagaaaaac cacaccacgc cttacgctgg tctggtgttt gacatcaatg acaactggtc
     6001 gacctacgcc agctatacct ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa
     6061 atatctggct ccaatcaccg gtaacaacta cgagctgggt ctgaaatcgg actggatgaa
     6121 tagccgtctg accaccacgt tagccatctt ccgtattgag caggataatg tcgctcagtc
     6181 caccggtaca cctatccccg gcagcaacgg cgaaaccgcc tataaagcgg tggatgggac
     6241 agtcagtaaa ggggtggaat ttgaactcaa cggcgcaatt accgacaact ggcagctgac
     6301 atttggcgca acgcgctatc ttgcagagga taacgaagga aacgccgtta atcctaatct
     6361 gccacgcacc acggttaaaa tgttcaccag ctatcggttg cctgtcatgc cagagttgac
     6421 agtcggcggt ggtgttaact ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg
     6481 caccttccgc gccgagcaag gtagctacgc gctggtggat ctcttcaccc gctaccaggt
     6541 gacgaaaaac ttctcgttac aggggaacgt caataacctg ttcgacaaaa cctacgatac
     6601 caacatggaa ggttctatcg tctacggcgc accgcgtaat ttcagcatta ccggcacgta
     6661 tcaattctga ataacacctg taaaaaaggc agccatctgg ctgccttagt ctccccaacg
     6721 tcttacggat tagtggttac ggatgtactc atccatctcg gttttcaggt tatcggattt
     6781 agtaccgaaa atagcctgaa caccagaacc agcaaccact acgcccgctg cgcccagttt
     6841 cttcaggcca gcctgatcca ctttagacac atcagcaacg ctgacgcgca gacgggtaat
     6901 acatgcgtcg aggttagtaa tgttttcttt accaccaaat gcagcaacca gagccggtgc
     6961 catttcgctg gtacctgttg cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt
     7021 cagatccagt gctttaatca gcacgcggaa gatggtgtag taaacaatcg cataaccgat
     7081 accgacgatc ggtaacagcc acagtttgct gctgttacca gacagaacga tgaagtcgat
     7141 cagaccgtgc gagaacgacg taccgtcacg catccccaga agaatacaga ttgggaatgc
     7201 caggcctgcc agaatcgcgt ggatgatgta caggatcggc gcaacgaaca tgaagaagaa
     7261 ctcgatcggc tcggtgatac cggtcaggaa cgaggtcagc gccgcggaga tcataatacc
     7321 gcccactttc gcgcggtttt ctggtttagc agagtgccag atagcaattg cggcagctgg
     7381 cagaccgtac attttgaaca ggaagccacc agacagttta cccgcagtcg ggtcacccgc
     7441 catataacgc ggaatgtcgc cgtggaaaac ctgacctgct gcgttggtgt attcaccaat
     7501 ctgcatctgg aaaggtacgt tccagatgtg gtgcagacca aacggtacca ggcaacgttc
     7561 gatgaaaccg taaatgccaa acgcaactac cgggttctgg taagcagccc actgagagaa
     7621 ggtctggatt gcagaaccaa tcggcggcca aatgaaggac agcacaacgc cagtaaagat
     7681 ggcagccagg ccagaaatga tcggcacaaa gcgtttaccg gcaaagaagc caagatactc
     7741 aggcagctta atacggtaga aacggttaaa catgtacgct gcaatcgcac cggagataat
     7801 ccctccgagt acgccagtat ccgccaagtg tttagaggcg atttcttcag caggtaaatg
     7861 cagtaccagt ggcgcaacca cggccatggt tttaaccatg atgccatagg caacaactgc
     7921 ggccagcgcg gatacgccat cgttattggt aaagccgagg gcgacaccga tcgcaaaaat
     7981 cagtggcatg tttgcaaaga cggaaccgcc tgcttctgcc ataacatgcg atacaacggc
     8041 gggcagccag ctgaaattcg cggaaccgac gcccagcaga atacctgcga taggtagtac
     8101 ggataccggc agcatcagcg atttaccgac cttttgcagg ttagcaaatg cattcttaaa
     8161 cataattgag agtgctcctg agtatgggtg ctttttttac gttctcacgc gtggcaaggg
     8221 gggagagcct cgccgtgtac agggcatcta agcgcccttt atttattaca cagagtaaaa
     8281 taattcagtg ccaatttgtt tgacggctat cacgtttcaa cttcagatca ttggagaagt
     8341 tgcacatttt gacaaaacat atgtcacaat atttcaaacg cggttattca ccgcccaatt
     8401 ttcggcggtg aactttactc gttttagcca ttaattacga gctttaaaaa aactcattca
     8461 acggatggat tgaaggcggg aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt
     8521 tacctgcgcc agttcttcaa cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc
     8581 acgaaccatc gcaggttgat tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga
     8641 gtcagtttcc accagtaatc gatccagggg gacataacgc gcagcatcgc gcagttgctc
     8701 cgcattacgg aaggtcacaa tgccggaaaa ggagatgtaa aatccgagat ccagtaattt
     8761 acccgccgtt tctctgtcct ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt
     8821 ttcttcgcgc agaattgcca gcgtatcggc acgggcgtca cgggtatgga cgataaccgg
     8881 cttgttcagt tcacgaccaa tctggatatg atggatgaag gactcttgct gacgtacttt
     8941 agtttccggt gtgtaataat aatccagccc ggtttcaccc agcgctacaa caccctcttc
     9001 tgccgccaga cggcgtaaat cttctacatc gtagggatca ttctggttga gtggatgcac
     9061 gccacaagaa aatacgacgt tgtcacgttc gcctaccaga tcccgcatat gtaaataacc
     9121 cggtaatgtt gtggcgactg ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag
     9181 aacgtcatcc acgtccttat gcaaagattc ataatccaga ccatcgagat ggcagtgtga
     9241 gtcgactaaa aacatgatgt ctctcttaaa gatgaggaac cggtaacaca acgcccggtt
     9301 gcaggtaatg ctcaatacgc agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg
     9361 tgacagacat taactgttca cgaatgtggc aaacatcccc cagtatagcc tgcaggcacg
     9421 agggagaaag atggtttgcc agttcggcga ccaggcccgg cacatcaaca ttggtcacct
     9481 gcgcagcgcc atgatggcgt tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac
     9541 gcgccggagc ttgttcatga ttaagggccg ccagcagcga ataccagtcg cccgatggca
     9601 cgctatatgc caacgcctga cacaatgttt cacgagcctg ccagttatct ccctgaaaca
     9661 acgccagtgc cgcgccaggc gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct
     9721 gtgacattgt cacttcgcgt gaaagccaag tcacggcgta ctgttctgga ggcggcgcaa
     9781 ggtaatgtaa ccgacaacga ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag
     9841 ccaggaaaaa ccaggtttct gctggtggct cttcaagcgt tttcagcaat gcgttagccg
     9901 cggcgtcggt cagtaaggca gcatcggtta cccagacaac tttcgcacca cctaggcgtg
     9961 cgtgctcatt cagcttttcg gtgacctcac gtaccgcatc aatgcccagc gtatttttcc
    10021 ctttttcggg agccagggtg tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc
    10081 cacgacagtg accgcaactt ttgtggccct gcggttgttg gcagagtaaa taacggctca
    10141 gggcgtagat taaagcatca tcgcccatgc ccggtaacgc ctgaatgagt agcgcatggt
    10201 gacctcttcc ggcctgatag ctggctacca gtttttcgaa atcaggtcgt aaccatggat
    10261 accatctcat gcgtccaact ccttcaccca gtgggtcacg gtagtgcgga ttgcatccat
    10321 cacggcctcc agcggctggg tggcatcaat ggtatgaata cttttatctt gtgcagccag
    10381 ttccagatag cgggcgcggg tgcgattaaa gaaatcgaaa gactcttgct caatacgatc
    10441 cagctcgccg cgcgcacgcg cgcgttttaa gccaacttcc ggggtaacat cgagatagag
    10501 cgttaagtct gggcgaaaat ccccgagaac agcatcacgc agtgttgcca gcatatgttg
    10561 gtcaatacca cgtcctccgc cctgatacgc ctgagtggaa agatcgtggc gatcgccaat
    10621 cacccaggtg ccgttagcca gcgcaggttt gatgaccgtt tctaccagtt gaacgcgcgc
    10681 ggcataaaac atcagaactt cggctttatc ggtaatgact tcatcgccta ccgatttgat
    10741 atccagcacc aggcttctta acttttcggc aagttgcgta ccgccaggtt cccgagtgaa
    10801 aaccatgtcg cggataccca gttgctcgag cgtctcaacc accacattac gcgcggtagt
    10861 tttgcctgcg ccttccagcc cctcaatgac gatatactta ctgcgcattt ttttccttaa
    10921 gcactttcag ataatcctgc acagacttgt tatgactggc aagattggta ttaaacgtgt
    10981 gaccaccttt accatcggcc acaaaataga gatacggcgt ttttgccgga tgcgcagcag
    11041 ccttcagcga atccgccccc ggcgtagcta tcgcacccgg cggcaaaccg gtaatggtat
    11101 aggtgttata cgctgtcggc gtttccaggt ctgcacgaga aagtttgcca ttataacgct
    11161 ctcccatccc gtaaatcacg gttgggtcgg tctgcaggcg cataccaatg cgtaaacggt
    11221 tgataaatac cgaggcaacc tgatcgcgtt cactggcaac ggcggtttct ttttcgataa
    11281 ttgatgccat cgtcaccagc tgatttttat ctttataagg cagaccgtcc gcacgccctt
    11341 cccaggcgct atcgaccgct ttcaccattt tcttgtgaac tcgcttgagt aacgcgacat
    11401 cggtggtatt ggcggtatac atcaaggtgt ctggccagaa ccaaccttca atccactccg
    11461 ggttttccag ttcaagtgcc tgcgctacgg tggcgtactt atcgtcactc agcgtgtgct
    11521 tgatatacgg agcctcacgc aattgcttga ggtagtcgct cagacgcatc ccttctacca
    11581 gtcgcagcgg gaactgtgct tctttaccgc tttccaacaa tttcagcatc tcgcgcacgg
    11641 tcatctgcgg tgtaaagcgg taagtcccgg ctttaaagtg agaaagatcc ggttcgatac
    11701 gcagcagcca ttgaaaaacc cgtgggcgat tgatgatctt atcagcataa agctgttcac
    11761 cgagcgccag acgtccggtc cctggcttca gggtaaatat cgtctcttct ttgataagca
    11821 atttgctgtc ggcaagatgg cgaaccttcc agacgcccac accagcggcg atacccagta
    11881 ccaccagcaa taacaagata atcaataaca cttttttcat gactaattcg ggcgctcaca
    11941 aagtggggct aaatattcat ataacgttgc tgacgaaaag gtgacatcgc cacaggcacg
    12001 tacgggcatc actggcatta acgcattaca aataaccatc tcatctgcct gcaacgcctc
    12061 ctccagcgag gcttgcactt cgacaagctg ataagaggat tgtgccagta aacggataca
    12121 gaattgtcgc ataatgccgt taacacctgc ctgatccagt cgcggcgtat aaactacatt
    12181 gcccttccgc cagaacaaat tagccgcaca gcattccgta acccaccctt cgctgtcaag
    12241 gaccagcgcc tcatcagcgt ttgtctgctc aagatgagag cgaatcaata cttgctcaag
    12301 ccgattaaga tgtttaatac ctgcaagatg aggattgcgc cccagccgca ccgggcttag
    12361 cgccaacgtc atcccttcgt tacgcaaacg gtcgtaatgt gcaggataag ccgtaacgga
    12421 gagaatccgc gttgctggtc cgctgttcaa tgtgctgtac cctcgcccgc cactaccgcg
    12481 actgatcacg actttcagca caccattttg ctgttctgct gccagcgttt tcatctcttg
    12541 ttcaagctga ggccagaagt cacaggaaat catcaaccgc tgacaagcat cctgtagtcg
    12601 caggatatgc gccgataaca aactgacttt accgtcgata actctggcgg tggtaaaaca
    12661 accatcacca aactgcgttg cccgatcgct tactgccagc gattcctgct tataaccgtt
    12721 aattaagaac ataccggctc cttatcagag aattgttagt gtggcagcat gttcactacg
    12781 gaacaagtcg gaataaaagc taagaaaaaa ggcccgcaag cggacctttt atatgggtgg
    12841 aaaatgacaa cttagatctt tttaaagatc aaagaaccat tagtgccacc gaagccgaag
    12901 gagttacaca gcgtgtattc cattccgctg acctgacgtg cttcatgcgg tacgaaatcc
    12961 agatcgcaac cttcatccgg gttatccagg ttgatggttg gcggaacagc ctgatcgcgc
    13021 agcgccagga tggagtagat agattctact gcacccgccg cacctaacag gtgaccggtc
    13081 atagatttcg tggagcttac caacacacgg cttgcagctt caccgaagat ggttttcacc
    13141 gcctgcgctt cagctttatc gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca
    13201 atctgactcg cttcaatgcc tgcatcacgc agagcatttg ccattgccag agctgcgcct
    13261 gcgccatttt ctggcggtga cgtcatatga taagcatcgc tgctcatacc aaagccgacg
    13321 agttcagcgt aaattttcgc accgcgtttt ttcgcgtgtt cgtactcttc aagtaccagc
    13381 ataccggcac catcgcccag tacgaagcca tcacgctctt tatcccacgg gcggctcgcc
    13441 gcttgcgggt tatcattgcg ggtagataat gcacgtgccg cgccaaaacc accaacgccc
    13501 agcggcgtac tggctttctc tgcgccacct gcaaccatca cgtcagcatc gccatacgcg
    13561 ataatacgcg cagcatggcc aatgttgtgc acgccggaag tacaggcggt cgcgatagag
    13621 atgcttgggc cacgcaggcc atacatgata gtcagatgac ctgccaccat gttcacaatc
    13681 gttgacggaa cgaagaatgg gctgatctta cgtggaccac cgttcatcag agatgtgtgg
    13741 ttttcttcga tcagtccgag gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt
    13801 gcgttctctt ccgttatttc aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt
    13861 ccatattgaa tgaaggcatc catcttgcgc tgttctttgc gcgagataat gtcctcacag
    13921 ttaaaatcct ttactaagcc agcaaatttc gttgcatagg cgctagtatc gaaatggtcg
    13981 attaggctga tgccactctg accggcaagc agagctttcc aggtagactc tacggtattg
    14041 ccgacaggag acaacatgcc cagtccggtc acaactacac gacgcttaga cacgtttgtc
    14101 ctccaaggag ggaaaaatga ttctagtggg acaaaaagat aaaactcagg cggtcgaacg
    14161 accgcctgga gatgttcact tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa
    14221 cggtggtgat tttctcagct tcttcgtccg gaatctcagt atcaaactct tcttccagag
    14281 ccattaccag ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat
    14341 tgttggtaac ttcttcctgc ttaacgccca gctgttcgcc gataattttc ttaacgcgtt
    14401 cttcgatagt gctcatactc ttaaatttcc tatcaaaact cgctttcgcg atggttttcg
    14461 tagtgtataa aatgttgaaa aatttgcaac taaatcccgg caggtcttac cacgatttta
    14521 cgttattttg aggccttacc ccccaataac gcaaatcatt ttgcactaat tattactcat
    14581 aaccacgcag actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag accatgtaca
    14641 tcccgccgtt cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga
    14701 atgcaaccgc gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca
    14761 ggatacccgc acgctggtca tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg
    14821 gagcaacaac gtttacagta ataccgcgtg acgcaacttc gcgcgccagt gatttactga
    14881 agccgatcaa gcccgctttc gccgcagcgt agttggcctg accgccattt cccatggtac
    14941 caaccacaga accgatagtg ataatacgac catgacgctt tttcatcata gcgcgcatta
    15001 ccgcttttga cagacggaaa acagatgaaa ggttagtttc gataatatcg ttccactctt
    15061 catctttcat tcgcattaac aggttatcac gagtgatacc ggcattattg accaggatat
    15121 ccacttcacc aaattctgcg cgaatttttt ccagaacaga ttcgatagat gccgggtcgg
    15181 tcacattcaa catcagacct ttgccgttgg cacctaaata atcactgatc gcctgagcgc
    15241 cattttcact ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa
    15301 ttgcgcggcc aattccgcgg cttgcaccgg ttaccagtgc gatttttcct tcaaaattca
    15361 tgattttcct cttttaaagc tcgagcgccg ctgccatcgc tgaaggttcg ttcagcgccg
    15421 aggcggtcag ggtgtcgaca atgcgtttcg tcaggccagt aagcaccttg cccgggccga
    15481 cttcatagag atgttctacg ccttgcgctg ccatgtactc aacagacttc gtccactgaa
    15541 ccgggttata caactgacgt accagtgcgt cacggatggc atcaccattg gtttcgcatt
    15601 tcacatcaac gttattcaca acagaaactg ttggtgcgtt aaaggtgatt ttcgctaatt
    15661 ctactgccag tttgtcggct gctggtttca tcagcgcaca gtgagacggt acgctcactg
    15721 gtaacggcag cgcgcgtttt gcgccagcag ctttacaggc agcgccagca cgctcaaccg
    15781 cttctttatg accggcaata accacctgtc ccggagagtt aaagtttacc ggagaaacga
    15841 cctgaccttc tgcagcttct tcacacgctt tcgcaatagg cgcatcatcc agaccgatga
    15901 ttgccgccat agcgcccgtg ccttccggta cggcttcttg catgaactta ccgcgcatct
    15961 caaccagacg caccgcatca gcgaaatcaa tcacgccagc gcaaaccagc gcggagtatt
    16021 cccccaggct gtgaccggcc atcattgccg gtgctttacc gccctgctgc tgccatacgc
    16081 gatacagcgc aacagatgca gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt
    16141 cttcagctga cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt
    16201 cagcaaacgt ttcttcgaca attggatagc tcgccgccat atcagccagc attccaacgg
    16261 tttgagaacc ctgtccaggg aacacaaatg caaattgcgt catgttttaa tccttatcct
    16321 agaaacgaac cagcgcagag ccccaagtga atccaccgcc aaaggcttca agcagaacca
    16381 actgccccgg cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg
    16441 ccgcagaggt attaccgtgg cgatccagcg tcaccacgac attgtccatc gacataccga
    16501 gttttttcgc cgttgcactg ataatacgca ggttagcctg atgcggaacc agccagtcca
    16561 gttgagaacg gtcaagatta ttcgccgcca gcgtctcatc aacgatgtgc gccagttccg
    16621 ttaccgcaac cttgaagact tcgttgcccg ccatcgtcag atgaattgaa ttctctggat
    16681 tcacgcggtc ggcgtttggc agcgtcagca attcaccata actaccgtcg gcatgcagat
    16741 gggtggaaat gattcccggc tcttcagagg cagccagcac cgcagcgccc gcgccatcgc
    16801 caaaaataat aatagtccca cgatctgttg gatcgcaggt gcgcgccagt acatcggaac
    16861 cgacgaccag agcatacttc accgccccag atttcacgta ttgatcggct acgcttaatg
    16921 cataggtgaa acctgcacag gctgctgcaa cgtcaaatgc cgggcaacct ttaatgtcca
    16981 acatgctttg aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca
    17041 caacgatcag gccaatctgg tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg
    17101 cttcaaagcc catggttgaa acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac
    17161 cggtacgagt gacaatccac tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg
    17221 tccgcacttg ttcgggcaga tagctgccag taccaataat cttcgtatac atgtacgctc
    17281 agtcactttt cggttatata ccgtcacttg caaactgcga gttcgctggc agcgtcctgc
    17341 taccgcagag ttccgctttt gccaccgtcc agcagctcaa aaccagctgg gtatacagat
    17401 tccaggcgag cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca
    17461 atcgcgaccg caaaagctcg ctgattggct gcaccatgac tttttatcac cgtgccgcgc
    17521 aatcctaaca gacaggcgcc gttatactgg tcggggttga ggtgactgaa tcgcctcgtc
    17581 aggctctttt gtagccaacg ctttaataac agtagccacc acgaccgttt tttcccttca
    17641 ccctgagatt tcagcagaga aaggaacatc ctgacaacac cttccatcgt ctttaacgtg
    17701 acatttcctg taaagccgtc acaaaccagc acatctgtct tgccagttaa caactcattg
    17761 gcttcaagat agccgatata attgatagaa gggattgttt taagcaccgc tgaggcatcc
    17821 cgaatactgt caagaccctt tacttcttct tcaccaatat tgagcaacgc cacgcgagga
    17881 ttgggaattt ccaccacctc ttcagccaga actgagccca taatggcaaa ttgcaccaac
    17941 attgtgctgt cacaatcgac gttggcccct aaatcgagga ccaccgtttt gcccttttgc
    18001 tgatgtggta ataccgtcac cagcgccgga cgctcaatcc cctccagggg cttgagtaat
    18061 aattttgcca gccccatcag tgccccggta ttaccggcac tgacacaggc ttgcgctcga
    18121 ccttctttca ccagctccag ggccatacgc attgaactcc cacggctggc gcggatagct
    18181 tgcgaaggcc gggcatcact ggcgataact gactgcgcag gaataatctg cagacgcgaa
    18241 cgttgttcaa agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa
    18301 agaagagtga gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa
    18361 gggccaaaat cccctcccat gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg
    18421 ccgcccggtt tggtctttcc ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc
    18481 agacgcgtga ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt
    18541 ggtgacgcag gtgtttttca ccagaagttt tgtctacaga caggctggtg actgcggtca
    18601 gcgcgtcatg ggaacgacgc atgccacgtt tggaacgggt tggtttattc tgttgtacgg
    18661 ccatggacct tactcctcaa ttacggttta tccccgatcc aacggggagc accaattact
    18721 tacgctttaa gctggctaat acggcaaatg ggtttggctt ttgcacttct tcaggcagtt
    18781 caccaaagac catgtccgct tcggacactt cacagtgttc agaatcatgc accggaacta
    18841 ccggcaaggc gaggatgatt tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt
    18901 cgttaacctc aatcggttca tacgcttccg gcagtgcttc agcctgttcg tctgaacgca
    18961 caggactaaa acaatacgtt gtgtagacct gatgagtaaa cggcttcccg caacgctgac
    19021 actcgagcgt taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga
    19081 tagcgaacga catggagcat tccacatcac tgtccacact gactacggat tcggcgacgc
    19141 gctcaacctg atcaggggta tagataccct ggtaatcaag gcgtttttga gccgtacgaa
    19201 ccggatcgag agtcaggggt aattttacct tttgcatagg gcgcgcatat taactttgta
    19261 acgtcataga gtcaaagaaa aaggcagcct gtggttgcct tttgccaata attcgcacac
    19321 attgcgggtt actgctttat tttcgttcag tgctgataac gaacatcaac ctgcaaaacg
    19381 aagcgcatag tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc
    19441 taaacttatt ttagcctcca cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat
    19501 ctcttttgaa tgtgcagcac ctgaggtcga cgaaacccca cgcagcgacg aatcaccgcg
    19561 acagttggtg cttcgactgg cacaagaaaa atcgcaatct ctggcgtcac gttatccgga
    19621 tcatttaatt attggttcag accaggtatg tgttcttgat ggcgaaatca ccggtaaacc
    19681 gttaacggaa gaaaatgccc gtctgcaatt acgcaaagcc agcggcaata tcgtcacctt
    19741 ctatactgga ctggcgttgt ttaattcggc gaatgggcat ctacaaacag aagtggagcc
    19801 ttttgacgtc catttccgtc acctaagcga ggcggagatt gataattacg tgcgtaagga
    19861 gcatcccctg cactgcgcgg gtagctttaa gagtgaagga tttggcatta cgctgtttga
    19921 gcgtttagag ggacgtgacc ctaacacgct ggttggttta ccgctaatcg cgctgtgtca
    19981 gatgttacgt cgggaaggga aaaacccgct gatgggatag ttgtttgcgc cggatgcggt
    20041 tacgtcgcat ccggcaatcg gtgcaggcgg cctgatgcgg cgtaaacgcc ttatcaggcc
    20101 aaccgcccat tatcaggctt atatgcttgt attagcgcgc gttacgcagc ttttgcaaac
    20161 aacgcttcaa accttcatcc atcggcgctt cgatacgcat cacctcaccg gtccccggat
    20221 gggtaaactt caacgctgcg gcgtgcagga acagacgatt taatcccgtg cctgcttcag
    20281 tgagctgtct gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg
    20341 catactgtgt atgcacacgg atctgatgag tacgtccggt taccgggcta caacgcacca
    20401 gggtggcaaa tgcatagcgt tcttccactt taaagcgtgt ttccgacggt ttgccttcct
    20461 gactgacacg cacgatacgt tcgccgcttt gcagaatatt tttcaataac ggcgcttgaa
    20521 cgctcttcac atgcgactgc cactgaccgc gcaccagcgc caggtaatct ttttgcattc
    20581 ctttttcacg taattgctca tgcagagaac gcaacgccga gcgttttttc gctaccagca
    20641 acacacctga agtgtcccgg tcgagacgat gaaccagttc aaggaaccgc gcttccgggc
    20701 gcaacgcccg caaaccttcg ataacgccga agcttaaacc actgccgcca tgtaccgccg
    20761 taccggaagg tttattcagc accaggatat gatcatcttc atataagatg acgtccgcca
    20821 gcgccgccac cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa
    20881 ccggtgggat acgcacctca tcacccgctt cgagtttata ttcaggctta atacgttttt
    20941 tgttcacccg cacttcacct ttacgcaaaa tacggtaaat catacttttt ggtacgcctt
    21001 tcaattgggt acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa
    21061 caatttttac ggatggagtc tctgttttca tggtgggcga ttctaattag ccaacaggat
    21121 tcgcgccact catttttcta tgcttatttt tagttttgca ccttattact tcactacgtg
    21181 atcactttat tgatggttat taaaccaatc accagcaaga agtgaaaaaa ctgtgagtaa
    21241 gcgggtgata aatggtaaaa gtcatcttgc tataacaagg cttgcagtgg aataatgagg
    21301 ccgtttccgt gtccatcctt gttaaaacaa gaaattttac ggaataaccc attttgcccg
    21361 accgatcatc cacgcagcaa tggcgtaaga cgtattgatc tttcaggcag ttagcgggct
    21421 gcgggttgca gtccttaccg gtagatggaa atatttctgg agagtaatac ccagtctgtt
    21481 tctttgataa ttgcgctgtt tttccgcatg aaaaacgggc aaccgacact ctgcgcctct
    21541 ttgagctgac gataaccgtg aggttggcga cgcgactaga cacgaggcca tcggttcaca
    21601 cccggaaagg cgttactttg cccgcagctt agtcgtcaat gtaagaataa tgagtaagtt
    21661 acgatgaaaa gaatgttaat caacgcaact cagcaggaag agttgcgcgt tgcccttgta
    21721 gatgggcagc gtctgtatga cctggatatc gaaagtccag ggcacgagca gaaaaaggca
    21781 aacatctaca aaggtaaaat cacccgcatt gaaccgagtc tggaagctgc ttttgttgat
    21841 tacggcgctg aacgtcacgg tttcctccca ctaaaagaaa ttgcccgcga atatttccct
    21901 gctaactaca gtgctcatgg tcgtcccaac attaaagatg tgttgcgtga aggtcaggaa
    21961 gtcattgttc agatcgataa agaagagcgc ggcaacaaag gcgcggcatt aaccaccttt
    22021 atcagtctgg cgggtagcta tctggttctg atgccaaata acccgcgcgc aggtggcatt
    22081 tctcgccgta tcgaaggcga tgaccgtacc gaattaaaag aagcactggc aagccttgaa
    22141 ctgccggaag gcatggggct tatcgtgcgc accgctggcg taggcaaatc tgctgaggcg
    22201 ctgcaatggg atttaagctt ccgcctgaaa cactgggaag ccatcaaaaa agccgctgaa
    22261 agccgcccgg ctccgttcct gattcatcag gagagcaacg taatcgttcg cgccttccgc
    22321 gattatttac gtcaggatat cggcgaaatc cttatcgata acccgaaagt cctcgaactg
    22381 gcacgtcagc atatcgctgc attaggtcgc ccggatttca gcagcaaaat caaactgtac
    22441 accggcgaga tcccgctgtt cagccactac cagatcgagt cgcagatcga gtccgccttc
    22501 cagcgtgaag tccgtctgcc atccggtggt tccattgtta tcgacagcac cgaagcgtta
    22561 acagccatcg acatcaactc cgcacgcgcg acccgcggcg gcgatatcga agaaactgcg
    22621 tttaacacca acctcgaagc cgccgatgag attgctcgtc agctgcgcct gcgtgacctc
    22681 ggcggcctga ttgttatcga cttcatcgac atgacgccag tacgccacca gcgtgcggta
    22741 gaaaaccgtc tgcgtgaagc ggtgcgtcag gaccgtgcgc gtattcaaat cagccatatt
    22801 tctcgctttg gcctgctgga aatgtcccgc cagcgcctga gcccatcact gggtgaatcc
    22861 agccatcacg tctgcccgcg ctgctccggt accggtaccg tgcgtgacaa cgaatcgctg
    22921 tcgctctcta ttctgcgttt gatcgaagaa gaagcgctga aagagaacac ccaggaagtt
    22981 cacgccattg ttcctgtgcc aatcgcttct tatctgctga atgaaaaacg ttctgcggtg
    23041 aatgcgattg aaacgcgtca ggacggtgtt cgctgcgtga ttgtgccaaa cgatcagatg
    23101 gaaaccccgc actaccacgt gttgcgcgtg cgcaaagggg aagaaacgcc gaccttaagc
    23161 tacatgctgc cgaagctgca tgaagaagcg atggcgctgc cgtctgaaga agagttcgct
    23221 gaacgtaagc gtcctgaaca acctgcactg gcaacctttg ccatgccgga tgtaccgcca
    23281 gcgccaaccc cagctgaacc tgccgcgact gtcgtagccc cagcaccgaa agctgcaacg
    23341 gcaacaccag cagctcctgc acaacctggg ctgttaagcc gcttcttcgg cgcactgaaa
    23401 gcgctgttca gcggtggtga agaagccaaa ccgaccgagc aaccaacacc gaaagcagaa
    23461 gcgaaaccgg aacgtcaaca ggatcgtcgc aaacctcgtc agagcaaccg ccgtgaccgt
    23521 aatgagcgcc gcgacacccg tagtgaacgt actgaaggca gcgataatcg cgaagaaaac
    23581 cgtcgtaatc gtcgccaggc acagcagcaa actgccgaga cgcgtgagag ccgtcagcag
    23641 gctgaggtaa cggaaaaagc acgtaccacc gacgagcagc aagtgccgcg tcgtgaacgt
    23701 agccgccgcc gtaacgatga taaacgtcag gcgcaacaag aagcgaaggc gctgaatgtt
    23761 gaagagcaat ctgttcagga aaccgaacag gaagaacgtg tacgtccggt tcagccgcgt
    23821 cgtaaacagc gtcagctcaa tcagaaagtg cgttacgagc aaagcgtagc cgaagaagcg
    23881 gtagtcgcac cggtggttga agaaactgcc gctgccgaac caattgttca ggaagctcca
    23941 gctccacgca cagaactggt gaaagtcccg ctgccagtcg tagcgcaaac tgcaccagaa
    24001 cagcaagaag agaacaatgc tgataaccgt gacaacggtg gcatgccgcg tcgttctcgc
    24061 cgctcgcctc gtcacctgcg cgtgagtggt cagcgtcgtc gtcgctatcg tgacgagcgt
    24121 tatccaaccc agtcgccaat gccgttgacc gtagcgtgcg cgtctccgga actggcctct
    24181 ggcaaagtct ggatccgcta tccaattgta cgtccgcaag atgtacaagt tgaagagcag
    24241 cgcgaacagg aagaagtaca agtgcagccg atggtgactg aggtccctgt cgccgccgct
    24301 gtcgaacctg ttgttagcgc gccagttgtt gaagaaatgg ctgaagtcgt agaagccccc
    24361 gtaccggttg ccgaaccgca accggaagtg gttgaaacga cgcatcctga agtgattgcc
    24421 gccgcggtaa ctgaacagcc gcaggtgatt accgagtctg atgttgccgt agcccaggaa
    24481 gtcgcagaac acgcagaacc gatggttgaa ccgcaggaag agacggcaga tgcagatatt
    24541 gaagaagttg ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct
    24601 gccgcgccag tcgtcgctga agtcgcagca gaagttgaaa cggtggccgc ggttgaacca
    24661 gagatcaccg ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa
    24721 tatgttccgg aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt
    24781 aaaggtgccg caggtggtca tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg
    24841 caacctgttg agtaataatt agctcaaagt aatcaagccc tggtaactgc cagggctttt
    24901 ttatttcatg tttgaatcaa catattaaaa ttcatcaaat ggcatccttg ctaaccaaca
    24961 atgcaaaata ggcaaaaaaa atgcccgccc cggcgatccc agagcgggca gacaaaacat
    25021 acccagtttc atgatatgtt ttaaagcgag cttatttgtt gagctggaag agcgacaatc
    25081 cctgcatatc ggtaaatgct ttatacgatg cctgcaatgc cgtttgctgc atgatgtaag
    25141 atgaaatagt tgcattccag tccacatcaa ccagatcgct catctgctgc gtttgcccta
    25201 aagcgcgatc gctacctaag gaatccagcg actccagttt gttcagctgc gtgcctaatt
    25261 ccgcgcgaac ggtaaggaca ttattcagcg agtttttcag tccacggttg gttttatcca
    25321 atgccgcagc ggcgatttct ttatccgctt cgctatccgc gaccggcgtt ttcagggctg
    25381 cgatggcgct atccagcatg gcaaaaagat tggtttcaga agcgctaccg tctggttccg
    25441 ctaccgcgtt actggtaata ctgtcgaaaa ttttgtcgcc cgtatgtcct atcaccatcg
    25501 aacgcgaagc atcgacctgt tgcttaatac tttctgttcc gccaacataa tcaccgttag
    25561 cctcactaaa cggcgcagtc tctgttttat atgtagcgcc cattaccgtc agtggtgttc
    25621 gccagattca gcaactggtc acgaagcccc tgaatatccg tagccagcga ggcccggtca
    25681 tcgtcactca aggtgccatt gctggcgtag acaatttttt cctgagcatt ctggatagca
    25741 gtggtgacct ggctaagtac actctcttcc agtgacactt tttgagtggc gaaagtacgt
    25801 gccagcgtgt actggctgtt ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca
    25861 gcaatgggat cgtctgaagg gttaacgact cgcttacccg tcgacatctg ctcgccgtac
    25921 ttcatccatt ctgcctgaga attggtgatg ccgcgcatgt tttgctggta catcatctgg
    25981 gtactgaaac gcattttatc tccccttagc gaatgttaat cagcgcatca aaaatcgcgt
    26041 ttgccgtctg cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc
    26101 cgtactcctc atcgagattg acaccggaaa tcgactgctg ctgattggaa agctgcgtca
    26161 ccacattacc ttgcgtggtg ctactggttt tcaacgtcgc ggttttatta ccgatatcac
    26221 tcactaacga ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc
    26281 tttgcagatc cagcagggcc tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc
    26341 tcgccatcgc gattttcgct tcgtcggtga ttaatacatc catgttgacg atggcgtcac
    26401 ttactggttt cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca
    26461 gaccatcaaa tgccacttta ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg
    26521 caaggcgggt gacctgccac tgattattat cgaacgagat tttgtaatct gtcgccagta
    26581 ccacggaggc atcagttacc gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt
    26641 gcagaaccgc gggcttaccg atagtaaaga aatcttcacc agcatcgccg ttagcatcaa
    26701 atccggcttt gtgttggctg ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg
    26761 tattacgcgt ctggtccaga tcctgagaac ggaatgtcag aatgccgccc agcgacccgg
    26821 tattcagtaa tttctccggg atctcaatat tgcctgccgt cccatcaacg taagcgacag
    26881 tcgtacgaga agggtcggcg ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct
    26941 gaaccagtga gtaaccattg gcatcgtgat gttataagta ccgccatcct gaacgctgac
    27001 ttctacgcca acaatctggt ttaattcgct caccagttga tcgcgttgat ccagcagatt
    27061 gttaggtgac gcccctgccc ccacgcctgt caggcgcgag atttgatcgt tcaggctggc
    27121 aatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg
    27181 tttgtcctgg tcgcgcagat attgatcggt ggttttaaac tgattcacca acccttctga
    27241 tttcccaatc agcgcctggc gcgccgccgg gtcttccgcg ttactcacca gcgtttgcag
    27301 gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt
    27361 gtcgattttc gacatctgct catagcgggc ggtcaggccg ctactttgcg tctgcgcagc
    27421 acgtaactgg ttggtaataa acgtatcata ctcacgctgc acaccagaaa cgtagacgcc
    27481 attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa tagtggtttg
    27541 gcgggtatat ccggcaacgt tatagctgga gatattatta cttgccgtat ttaacgccgc
    27601 ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta
    27661 taagccttca attacagagt attatcggca gcgacccgcc ggacttgagt tattcagaac
    27721 agattatcaa tgttcatgct gtaggttttg ctcaccttgt cgcttatcga tttcatctgc
    27781 tggatcatgt tggtgagttt acgggcatag tgaggatcgg tagcatagcc cgcgtcctgt
    27841 aggacctgcg ccccctgttc cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta
    27901 cgtgttaaca gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaac
    27961 ttcgctttta ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat ttcagtgact
    28021 tgccctttcc agttgccaga ggctttgaca ccaaacaggt tatagcttgg ctcgccgttt
    28081 tcgcggcgga tttgccgttg cccccaacca gattcaagcg cagcctgagc gaggatcaaa
    28141 tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg
    28201 aatgctctac tgtcacccgg cagcgaatca tcgtagttac gtggtacggc cttttgcacc
    28261 agctgcgaaa gcgtctggtt ttgataacgc accacggttt cgagcgggaa tttcatcggt
    28321 gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgttt aaccatcatc
    28381 tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatttgctg gtcatacata
    28441 ctggtataca gacgagtgtg ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc
    28501 atgcttttca acatcatctg cacgaacatc ccttccacct gacgggccac cggacggata
    28561 tttgccgccg gatcttcgct cgctttcgcc tttagttcgt tgagtgattg cgcatcccag
    28621 gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca
    28681 gacatcccgc actttgcatt gattgcaaaa tagacatcag atccatcggc gtagcgccca
    28741 gcgcattgag cgcacgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg
    28801 aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aacaacagtc tgtccaccac
    28861 caaacggtgt atctggctgg ctgacattgg cctgacgatt aactgttact gagagatttc
    28921 cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt catcaccacc gaaccggtgc
    28981 gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat
    29041 cggcaaggaa acggacctgg gaactgttgc cacttggtac gcgcacctga atagtccgcg
    29101 catctaacgc ggtggcgctg ccatatccac gcacgcggtt gatggtgtca gcgatttgct
    29161 gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact
    29221 ggctgggcaa ttcacgttca ataaccgcac cattggtgat ccgtccaccg ttcagttggt
    29281 taacctgaac actgctaccg cctgcggagg ctcctgcgcc gccaaccaga atattgccct
    29341 gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg agtcatcaac aacgtacctc
    29401 cacgcaagct tttggcatta cccatggaag aaaccaccac atcgatggtt tgcccctgac
    29461 gtccaaacgg aggaagtgac gctgtcacca ttaccgcagc gacgtttttt agctgcatat
    29521 tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg
    29581 taaacggcgt ctgggttgtc tggtcaccag tgccatccag ccccactacc aagccatagc
    29641 caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct
    29701 gagccgccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc accacagcct
    29761 cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc
    29821 gttaatgtag ccattgccta cgtattcaat acgcgcatcc gccacctgag tagacggtac
    29881 ggtattgctg ccgctgatag tgcgtggatt aaccacgccc gagaagcgaa taaattcggt
    29941 accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt tgaccagtac
    30001 ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg ctggcattgg ccccgccctt
    30061 tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacagccc
    30121 ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt
    30181 cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc
    30241 aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc
    30301 agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt
    30361 cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa
    30421 caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa
    30481 tcagtaaacc ggtgaacctg ccaccggtta tgccttagag ttgcgtcagt ttttgcagca
    30541 tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca
    30601 tattgaccag ttcttccgcc acgttgacgt tagacgtttc aacataccct tgatacagca
    30661 gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg
    30721 tgtagaggtt ttcgccaatg ctctccagtc cggtatcatt cataaaggtg gtgagattga
    30781 gctgcccaac ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac
    30841 gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac
    30901 caccagccgt caccagctgc ccgttctgat ccacctggaa agagccgtca cggatatagg
    30961 ctgatgagcc atctggcaac attacctgga aaaagccctg ccctttaatc gcgacatctt
    31021 tgctgttatt ggtctgcgac agattgccct ggctgtgtaa gcgttcagtg gcgaccgggc
    31081 gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc
    31141 ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac
    31201 cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt
    31261 caaggcccgt tttggcgatc cataatgaac tgatcatata atgtcctgta gtggattgct
    31321 gacaagcaga gcagaagcac gcgacagtgt gaatttcccc tcaccctaac cctctcccca
    31381 aaggggcgag ggaatagtcc gcgcgctttt attgcaatgt catcaatcgt tcaattaact
    31441 catcgacagc agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat
    31501 ctgcatttca aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac
    31561 gttactgcct tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg
    31621 ccctcgcgtg gcctgggttt ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac
    31681 ttcgctgccc gtggctttca ccagtttcag acgccctact ggcgcaactg tatttgccgg
    31741 atcgcccgga ttgagcgccg agattgtgcc atcggcagca atcgtgattt ccgccccttc
    31801 cggcacagca attggccccg cctcgcctat caccggatgc ccctgaattg tcagttgccc
    31861 ggtgggatca acctgaatgc tgccattacg cgtataccct tcgctgccgt cagcggtctg
    31921 cacggccagc catcctgctg caacgcaacg tccagcgggc gagaggtgta atccatcttg
    31981 ccgggcgtca tgtctgcacc cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa
    32041 agcccttcca ccggcactgc gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag
    32101 gcattggcca gattactggc ggttaccgcc tgttgattca gcgtctggct ggctgctccc
    32161 atcgcagtat aaattgcgtg atccattgag ctatcccgtc agcgattagc gtaagttaac
    32221 ccgcgtgttg aggatctggt cctgggtttt gatggtctgg gcgttagact tatagttacg
    32281 ctgggcaacg atcatattga ccagttcttt actgagatcg acgttggacg cttccagcgc
    32341 accgttggtc agagtgccaa agtttcccgt cccggctgtc cccaacagcg ccacgccaga
    32401 agattgcgtc gcagaccaga cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa
    32461 gttcgccagt acaatctgcc ccagcagttg ggtttgttcg ttggaattgt tgccgacaac
    32521 cgtaccgtca tcattgattt gataactcac cagatcgccc ggtttgtagc cattctgggt
    32581 ggttgccaca atgttgttag cgccggttgc gatattattc gccatcgcac catccactaa
    32641 tgtgccatta gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact
    32701 gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaaat agacgctcat
    32761 gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc
    32821 cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat
    32881 ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt
    32941 ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttaccgggt aacccgtcag
    33001 ctgtaaacct tgcgtattca acaggttacg gttttcatcc agcttaaatt gtccgttacg
    33061 gctgtagaac accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc
    33121 aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc
    33181 ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt
    33241 gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt
    33301 ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa
    33361 tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag
    33421 atccagcgta ttaccgctgt tgccgcggat cacaccctgc accagagcaa actgcagcgg
    33481 ctgggcaacc agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt
    33541 cggcgcagtg ctgccatcag tcaacgaacc gtcccaggtg aaactgtgaa ctccggcggt
    33601 cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt
    33661 aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc
    33721 ttcttcactg ccggttcccg caagaacagt tgtgccgggg atcatcacgc cgtgaccgat
    33781 caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt
    33841 ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc
    33901 gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca gcaaagtcag
    33961 aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact
    34021 gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat
    34081 tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg
    34141 gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc
    34201 ggcattttta tgtagccctt tgcatctgcc agcggattac ctggttcata aaccagtttg
    34261 tccggggcct gactttctta acatcggcaa cctttacgcc gcctgtcgca gcacctggtg
    34321 cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca
    34381 cgctatcagc atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta
    34441 acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta
    34501 aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca
    34561 ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacggtatta ccgtcaagcg
    34621 aaggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc gcctgcgccg
    34681 ggatgtgctg cgttgaggtc atcgtcagtg caaccacact ggttgcatcc cgtccacgtt
    34741 gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat
    34801 cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct
    34861 cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg caggtatcaa
    34921 aattctgcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata aacgcaaaat
    34981 gggtcgctat ttatgccgtt gatggtcatt gcggacaggt acaattcacg ttgtagaaat
    35041 ggctgggggc gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg atactgttca
    35101 gtccgttaag tacggcgagc aatctcacat cgcaattgca caactttttt agcgcccaac
    35161 tcgcgggggt aagtgatgag gttcgtgttt ctattcgtac agcgcccaat ctactaccgc
    35221 catgcgagca gccattgctt tcgatgagca ataattcccg cctgtggggc aatgtgaatg
    35281 tgttggcacg ctgcggtaac gacaaacgat atttacaggt taatgtgcag gccacaggaa
    35341 attatgtggt tgccgcgatg cctattgtgc ggggaggaaa gctggaagct ggcaatgtca
    35401 aactgaaacg tggacgcctg gataccctgc caccgcgtac ggtgctggat atcaatcaac
    35461 ttgttgatgc cgttagcctg cgcgatctat cacccgatca acctatccag ttaacccagt
    35521 ttcgccaggc atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc agcggtgatg
    35581 ggtttagcgc caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca cagaatgcgc
    35641 gggtgcgcat ggtatcggaa caggtagtca gcggcgttgt tgatgcagat gggaatattc
    35701 ttataaacct gtaagctgtt aaagattacc cgtcccttgc cgataaataa gcaacacatg
    35761 ataaaagcgc cctcaatgag gaataaacca tgagtattga tcgcacttcg cctctgaagc
    35821 ctgtaagcac cgttcaaccg cgcgaaacca ccgacgcgcc ggtaacgaac agccgggcgg
    35881 caaaaacaac cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga
    35941 tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc
    36001 gtaacggtga actaaaaatg gacaccggca aaattgccga tgcgctgatc aacgaagcgc
    36061 agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct cgaccagatg
    36121 tccgctgtgc ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg
    36181 gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg
    36241 acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat
    36301 attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa
    36361 cataacggct ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg
    36421 aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac accccatcgc
    36481 ggcagtaaaa agatttcgat ctgaatatta tctccggcct gcactgcagg ccggaatgca
    36541 ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc
    36601 gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat
    36661 ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac
    36721 cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat
    36781 tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat
    36841 agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt
    36901 taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa
    36961 gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa
    37021 cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac
    37081 ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagtgcca acaggaaaca
    37141 aagacgcaac ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact
    37201 tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac cggagggaaa
    37261 ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc
    37321 aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc
    37381 catctgtttc accacgcgca ttgctccagc atcgtggaag ttaatgcgcg gcaggaccag
    37441 catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcagaatgc cgccgaccgt
    37501 aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa
    37561 accaatcatg ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca
    37621 cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt
    37681 aatctttagt agctggctgg tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc
    37741 ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc
    37801 aagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt
    37861 acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat
    37921 acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc
    37981 gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt
    38041 catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa tccggaccca
    38101 cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa tagtctacct agattatggt
    38161 gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt
    38221 caacgatacg ttgcgccagc actgcctatt cgccggcggt ttgcggaact gtctgatttt
    38281 acacgcattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgctca agagtgctct
    38341 gccaaccagg aatcggttta ttcactacac cctgcccgcg ctcctcacgc cattcgcgca
    38401 tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc
    38461 cggcacggcg atgcatacaa gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg
    38521 caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc
    38581 cgcccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat
    38641 aaagatcgtg tggcccgacg ctattgctac gatgtttatc cattcttagc gaggctgcgg
    38701 tggcgagttg cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca
    38761 tcaacgtcag ttttttccgc gccgccagtt ccaccagcct ttcagcatcg cgcagatttt
    38821 ctgccagcgg tttatcgaca cagacatgta cccccgcatt gagtaacgta ctgaccacgt
    38881 caaagtggct ggcggtgctg gagtgcacaa aaaccgcatc acaactggcg gcaaggctgg
    38941 ataacgaatc ggcataagga atgcgccagc tgtcacaaat tggcagggct ttggcgcgcg
    39001 taggcgacca ggctccttgt aacgtccagt cagacgctgc cgccagcacc ggtaaccacg
    39061 ctttttgcgc aatgccgcct aatcccacta cgccgatacg taattttttc actttaatct
    39121 cccagatggg ccagcaacga atcaagacgt tgtttcagtt ctgccacttc gatttccagg
    39181 gcttcgacgc gggtctgtaa atcaccgtca accatgttcg acatagccgt taccgccggc
    39241 tgatcttcaa cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg
    39301 cgggccagac gcaccacaaa aggaccatct tcgcgattcg ccagttgttc cagcgtcgac
    39361 tccacttccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcaccc
    39421 ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttctgc ggcgctcagt
    39481 ttcagatcgc caaattctga attacaaaaa cgttgctcat atttggtgac ccgattacca
    39541 aaaccgctca ctgtgcgtag ataatgacgt ttgaccagat tatccagctg ttcctgcact
    39601 tcggattcac tcagattcat gaccggttca cggttcgttt tctgattaca ggccgtgact
    39661 acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa
    39721 ccaattacgc gagcttccag ggcagtaagt tgatatttca tgagattctc cttagcggcc
    39781 gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat
    39841 caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc
    39901 gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg
    39961 catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg
    40021 cccaatcgaa taaccgagat agcaggcatg aaaagagccg cgaacaacat tggaaaaatt
    40081 ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc
    40141 ttgtttatga aattcgttaa tcatccctag cctggcctgc cagcctgatg gataacagtg
    40201 gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc
    40261 cgccagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa
    40321 gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac
    40381 gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa
    40441 aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt
    40501 gataaaaaaa tactgtcccc gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa
    40561 agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct
    40621 gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt
    40681 cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat
    40741 tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg gcgcaattgc
    40801 cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt
    40861 cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg
    40921 actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc
    40981 acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgcca gtgcggtcat
    41041 tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg
    41101 ggccgttttg tttgtgctat gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact
    41161 ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt
    41221 tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgat
    41281 gctgccaatt atggtcaacg acgtggctgg cgcgccctct gccgttaaat ggatgtatgc
    41341 cattgaagcg tgtctgtcgt taacgttgct ctaccctatc gcccgctgga gtgaaaagca
    41401 ttttcgtctg gaacaccggt tgatggctgg gctgttgata atgtcattaa gcatgatgcc
    41461 ggtgggcatg gtcagcggcc tacaacaact tttcaccctg atttgtctgt tttatatcgg
    41521 gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc
    41581 tcgcggcagc tatatggggt ttagccgtct gggtctggcg attggcggcg ctattggtta
    41641 tatcggtggt ggctggctgt ttgacctggg caaatcggcg caccagccag agcttccgtg
    41701 gatgatgctg ggaattattg gcatcttcac tttccttgcg ctgggttggc agtttagtca
    41761 gaaacgcgcc gcgcgtcgtt tgctggaacg cgacgcctga tttgatactc ccccctgact
    41821 cctccatact gaaagtgaac tgacagccaa atggaggagt cacgtgaagc tatacattta
    41881 cgatcactgc ccttactgcc tcaaagcccg catgattttc ggcctgaaaa atatccccgt
    41941 cgaattacat gttctgctca acgacgacgc agaaacaccc acccggatgg tcggtcaaaa
    42001 acaggttccc attctgcaaa aagatgacag ccgctatatg ccagaaagca tggatatcgt
    42061 tcactatgtc gataaactcg acggcaaacc gttactgacc ggcaaacgtt cccctgccat
    42121 tgaagagtgg ctgcgcaagg tcaatggcta cgtcaacaaa ctgctgttgc cgcgttttgc
    42181 caaatcggca tttgatgagt tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa
    42241 agaggccagc gcgggtaatt ttgccgacct gctggcccac tctgacggtc tgattaagaa
    42301 tatcagcgat gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg
    42361 cgaactttcg gaagatgata ttcagctatt tccgctactg cgtaatctga cgctggtagc
    42421 cggaatcaac tggccaagcc gcgttgctaa ttaccgcgat aatatggcga aacagacaca
    42481 aatcaatttg ttatcatcaa tggcgattta atcctgaccc gccgcggcag cctgtattcg
    42541 cgggttgccg ccgttttcag ttttttctgc tggcgagttt atcgctggtg acacatgctg
    42601 cgttgtgtca ggatattgac gcagatttca tgtcgaggac taccatgaac aagtttttat
    42661 ttgccgctgc attgatcgtc agtggcctgc tcgttggctg taatcaactc acccaataca
    42721 ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca taataatttc tcaaaagata
    42781 tcggtttacc cggcgtggct gacgcccata ttgttctgac aaacttgacc agccaaattg
    42841 gtcgcgaaga gccgaataag gttaccctaa ccggagacgc caatctggac atgaactccc
    42901 tgttcggtag tcagaaagcg accatgaaac tgaagctgaa agcgctgccc gtgtttgata
    42961 aagaaaaagg tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg
    43021 aaaaaatgca aacggtgatg caaacgttgc ttccctattt gaaccaggca ttacgcaatt
    43081 actttaacca gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc gaagcaatgg
    43141 cgaaaaaact ggcgaaaggc attgaagtga agccaggcga aattgtcatt ccgtttacgg
    43201 attaatgacg agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct
    43261 tatcctttgt gtccggcaaa aacatccctt cagccggagc atagagatta atgactgcac
    43321 catcccaggt attaaagatc cgccgcccag acgactggca ccttcacctc cgcgatggcg
    43381 acatgttaaa aactgtcgtg ccgtatacca gcgaaattta tggacgggct atcgtaatgc
    43441 ccaatctggc tccgcccgtg accacagttg aggctgccgt ggcgtatcgc caacgtattc
    43501 ttgacgccgt acctgccggg cacgatttca ccccattgat gacctgttat ttaacagatt
    43561 cgctggatcc taatgagctg gagcgcggat ttaatgaagc cgtgttcacc gctgcaaaac
    43621 tttacccggc aaacgcaacc actaactcca gccacggcgt gacgtcaatt gacgcaatca
    43681 tgccggtact tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat ggtgaagtga
    43741 cacatgcaga tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc gtgatggaac
    43801 ctctgcgcca gcgcctgact gcgctgaaag tcgtttttga gcacatcacc accaaagatg
    43861 ctgccgacta tgtccgtgac ggaaatgaac ggctggctgc caccatcact ccgcagcatc
    43921 tgatgtttaa ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg tattgtttac
    43981 ccatcctcaa acgcaatatt caccaacagg cattgcgtga actggtcgcc agcggtttta
    44041 atcgagtatt cctcggtacg gattctgcgc cacatgcacg tcatcgcaaa gagagcagtt
    44101 gcggctgcgc gggctgcttc aacgccccaa ccgcgctggg cagttacgct accgtctttg
    44161 aagagatgaa tgctctgcag cactttgaag cattctgttc tgtaaacggc ccgcagttct
    44221 atggcttgcc ggtcaacgac acattcatcg aactggtacg tgaagagcaa caggttgctg
    44281 aaagcatcgc actgactgat gacaccctgg tgccattcct cgccggggaa acggtacgct
    44341 ggtccgttaa acaataaaaa tcgtagcttc ctgttgtcat taggttattt tacctgtata
    44401 aataaccagt atattcaaca gggggctatt atgcgaattg aagtcaccat agcgaaaact
    44461 tctccattgc cagctggggc tattgacgcc ctggctggcg aactttcccg ccgtattcag
    44521 tatgcgtttc ctgataatga aggccacgta tcggtacgtt atgccgcagc gaataattta
    44581 tcggttattg gcgcaacaaa agaagataaa cagcgcatta gcgaaattct ccaggaaacg
    44641 tgggaaagcg ccgatgactg gtttgtcagc gaataatatg cagtgatttt tgtttttgcc
    44701 ggatcgcccc ggctttttta ttttacttac gaattttgag tacaaaattt ctacacttcc
    44761 tcaaaataat tgcacaatat gctaaaaaaa tatgaatgac atgctgtttt attgttcata
    44821 taaatcttaa aagcagaaat ttatctgttc ccccctttaa aaattcggtg aataccctta
    44881 cttattggta tactgaagtt gcttcagata aaaacgcatt gaacctcgaa taacgttgtc
    44941 tagtaacacg aattaggggg ccatgatgga aaaaaataat gaagtcattc agactcatcc
    45001 gctcgtaggg tgggacatca gcaccgttga tagctatgat gcgctgatgt tgcgtttgca
    45061 ctaccagacc ccaaataagt ccgagcagga agggactgaa gttggtcaga cgctctggtt
    45121 aaccactgat gttgccagac aatttatttc gatattagaa gcaggaatcg ccaaaattga
    45181 atccggtgat ttcccggtaa acgagtatcg gcgtcattaa catgacatga ccatccaccc
    45241 aaaaggcacc tcaccggtgc ctttaccatt tctgggttgt atatctgcat tcagttaatt
    45301 actctgctta aaccatgaga gcagagagaa aaaaatgaaa tacgatctca tcattattgg
    45361 cagcggttcc gtaggcgctg ccgccgggta ttatgcaacc cgcgccggtt taaacgtgct
    45421 aatgaccgac gcccatatgc caccgcatca acacggcagc caccacggcg atacgcgatt
    45481 aattcgccat gcttatggtg aaggcgaaaa gtatgtcccg ttggtcctcc gcgcgcaaac
    45541 gctgtgggat gaactttccc gccacaacga agatgatccc atttttgtac gctctggtgt
    45601 cattaacctc ggcccggctg actccgcatt tctcgccaac gtcgcccaca gcgccgaaca
    45661 gtggcaactc aacgttgaaa agctcgatgc gcaagggatt atggcccgct ggccagaaat
    45721 acgcgtcccg gacaactaca tcggcttatt tgagactgat tccggttttt tgcgcagcga
    45781 actggcgatt aaaacctgga tccaactggc gaaggaagcg ggctgtgcgc aactgttcaa
    45841 ctgcccggtc accgcaattc gtcatgacga tgatggcgta actattgaaa cggttgacgg
    45901 tgagtatcag gcgaaaaaag cgattgtctg cgcgggaaca tgggtaaaag acctgctccc
    45961 ggagctgcct gtccagcccg tacgtaaagt atttgcctgg tatcaggccg atggccgcta
    46021 tagcgtgaag aataaattcc cggcgtttac cggtgaactg cccaatggcg atcaatatta
    46081 tggttttccg gcagaaaacg acgcgttgaa gattggcaaa cataacggag gccaggttat
    46141 ccattcagcg gatgaacgtg ttccgtttgc ggaagtggcc agtgatgggt cagaagcctt
    46201 cccgttcttg cgcaatgtat tgccgggtat cggttgctgc ctgtacggcg ctgcctgcac
    46261 ctatgataat tcgcctgacg aagattttat tatcgatacc ctacccggcc acgataatac
    46321 actgctcatt accggcctga gtgggcacgg ttttaaattt gcgtcagttt taggggaaat
    46381 agctgccgat tttgcgcaag accaaaaaag cgattttgat ttgacgccat tcaggctttc
    46441 ccgcttccaa taatcatcat tacggcctcc agaatgaggc cgtttttaca ggagtattta
    46501 atgcgtcggc tgttgcacta tctcatcaat aatattcgcg agcatctgat gctttatctt
    46561 tttctctggg gattgttggc aattatggat ctgatctatg tgttttattt ttagttttcc
    46621 gcactgacat aaactgacaa atatttaatc tccctttcat ttagcgtgaa aaattgaatg
    46681 agattattcc tgcttctaca gcactcacca gacatctctg atgctgaacc cactaaactt
    46741 atgaagattc tttcattatt aattcaaaat tattgaaata aaaataaata tcattcaatt
    46801 tgttattttt gtgttgcaat tcattctccg acacgccatt ttctttctct cgcaaattcg
    46861 tattggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt atatctgcgg
    46921 ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt ctgtggatgg
    46981 tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat aaaagcatcg
    47041 gtattttgtt aatgatgggg ctggttattc gcgttctgtg gcgtgtcata tctccaccgc
    47101 cgggtccgct gccgagttat tcgcccatga ctcgtcttgc cgcaaaagct ggacatctcg
    47161 cattgtgcct attgctgttc gccatcggca tcagcggcta tctgatctca actgccgatg
    47221 gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gacccttgcc gacgctggcg
    47281 cacaggcaga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc gtagtactgt
    47341 ctgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac gacactctga
    47401 agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa aaaagcctgc
    47461 ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc gccgattaca
    47521 aaattgacaa agaaggtcag cacgcctttg ttaacttccg catacagcat cttggctata
    47581 gctggttata tggcaccttt aaagatttcg acggtacttt tacctttgat gaaaaaaatc
    47641 cggctgccga taaagtgaat gtgacaatta acaccaccag cgtcgatact aaccacgccg
    47701 aacgcgataa acatcttcgc agtgcggatt ttctcaatac cacaaaatat ccacaggcaa
    47761 cattcacctc caccagcgtg aagaaagacg gtgacgaact ggatattacc ggcgatctga
    47821 cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt cagggcgacg
    47881 acccatgggg cggtaaacgt gcaggctttg aggccgaagg caaaattaag ctcaaagact
    47941 tcaatatcaa gacagattta ggtccagctt ctcaggaagt ggatctgatt atttcagtgg
    48001 aaggcgtaca gcagaagtaa gagtgaaatg ttgccggatg gcacgcatcc ggcaatgata
    48061 tttattctgt tggatcagga atacccagtg ttgtattcag acgtccacga gacttattaa
    48121 agatcttatt gccattttca cgtcctgccc gacggcgtcg ctgttcttct ggcggtaacg
    48181 cgctttcttc gcagcaaatc tcactacaac aacctttgta tttttccgcg catactggac
    48241 actgaataaa cagcaggtgg cagccatcat ttttacagtt ggtatggctg tcgcacggcg
    48301 cgccgcactg gtggcaatgc gcgataatct catccgatat acgttcgccc atccgctcgt
    48361 caaaaacaaa atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc gccttacggg
    48421 cgtattcaat aattccgccc tcgatatgcc agactttgtt gaatccgtta tgtttcatcc
    48481 aggcactggc cttttcacaa cgaatgccgc cggtgcagta catgacgatt tttttatctt
    48541 tatgtgcctg catcatctcg actgcttttg gcagctgctc acggaaggta tctgccggaa
    48601 tttcgagcgc gttttcaaag tgccccactt catactcata gtggttacgc atgtcgataa
    48661 atagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaga tactcaccaa
    48721 cattgctggc atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga tcgcgtacct
    48781 tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc aggcgtaaac
    48841 cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca ttgctcgcag
    48901 gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca aaaacattca
    48961 gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg atgtggaaat
    49021 acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc caacattttg gcttttaacg
    49081 cgtcgttgga aatgcggttg tgtaacactg gcatggtgta cggttcctgc gagatgggaa
    49141 agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca cattttacgc
    49201 tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg cccagtcttc
    49261 agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg cccgtatagg
    49321 ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact gcttcatccg
    49381 cgttattggt taacctggtt gggtattggc gtactttggt tggtcgtgca attgccctac
    49441 ccgattatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt tatgaaacga
    49501 cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag cgaacaagaa
    49561 cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggcctgat ggaaaccggt
    49621 atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat cggcatggaa
    49681 cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat ccattttctg
    49741 acactggagc tgggcgcgcg gcagtttggt atgcaggaac cggggattgg cgtttaccgc
    49801 ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg ctcaaataaa
    49861 tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa aggcgaagtg
    49921 gtctggtacg caccagatca tgattacggc ccgcgctcaa gcgttttcgt cccgttattt
    49981 gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat gtccggcgca
    50041 tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca attgattatg
    50101 ctgccaccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc gtggatgaac
    50161 aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt acaccgtcgc
    50221 tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccatgc cggatgcttc
    50281 agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa agctctctgg
    50341 attgcgcccc ctggaagtcg ggcgcataat tagtgtgttt atcttttctt cttatgttca
    50401 ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtgaaaatga cacccctata
    50461 aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg ggccgccttc
    50521 agtctggtaa tgcccttctt acccctctac gttgagcagc ttggtgttac cggccactcc
    50581 gccctgaata tgtggtccgg tattgtcttc agtataatgg gttcctaaac acatcatttt
    50641 aggtattgaa tatgagacgg gcaatcagct acatcagatt ttcaagtgaa cgacaactga
    50701 aaggggactc tgtacgccga caatctaagc tggttactga ctggcttgat aagaaccctg
    50761 agttttactt ggattcatcc ttgagcttta aagatttggg gaaaagtgct tttagtggta
    50821 agcacctgaa aggtggtcta ggtgactttt taaccgctat cgagaaaggg cttgttaaag
    50881 ctggtgatac gctactaatc gagagtttag accgtctgtc acgtcaggat attgatattg
    50941 cttctgagct attacgccgt atcttaaggg ctggtgtaga tgtagtcaca ctgagcgatg
    51001 gagaacatta cacacgagaa agtttaaaag acccgttagc cctgattaaa agcattctga
    51061 taatgcagcg ggcgcatgag gaaagtttga gaaagtctga gcgtgtccaa gctgcatgga
    51121 accgtaaaaa agaactcatc tccgaaggta tcaaggtatc taggcgttgt cctgcatggt
    51181 taaggctgaa tgatgaccgt cgaacattca caatcattcc tgataaagtg gaagttgtta
    51241 aacgagcatt tgacctgaga ttacagggtt taagcttttg ggctatcacc agaactttaa
    51301 acgatgaagg tcacttgtca ttgaatcaat acacacccaa acaaaagggg tggtctgata
    51361 ctgctgtaaa gaaactgctc agaaatcgtg ctgtgattgg atgtttcaca cctgctggac
    51421 gtgaagaagt tcagggttac taccccgcaa taatcagtga gtcactattc tatcgagtac
    51481 agcaactgaa cacagggcaa tatggtaggg cttctgtatc aagtaatccg ctatcagtca
    51541 acttgttcag gggtatcatc aa
//
LOCUS       DAZPEU010000007        49727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPEU010000007 DAZPEU010000000
VERSION     DAZPEU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.007"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            220..372
                     /gene="mokC"
                     /locus_tag="ACHU2L_000336"
     CDS             220..372
                     /gene="mokC"
                     /locus_tag="ACHU2L_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR1355343.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(476..1606)
                     /gene="dnaJ"
                     /locus_tag="ACHU2L_000337"
     CDS             complement(476..1606)
                     /gene="dnaJ"
                     /locus_tag="ACHU2L_000337"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR1355344.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1695..3611)
                     /gene="dnaK"
                     /locus_tag="ACHU2L_000338"
     CDS             complement(1695..3611)
                     /gene="dnaK"
                     /locus_tag="ACHU2L_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR1355345.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3988..4392
                     /locus_tag="ACHU2L_000339"
     CDS             3988..4392
                     /locus_tag="ACHU2L_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR1355346.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4418..5131
                     /gene="msyB"
                     /locus_tag="ACHU2L_000340"
     CDS             4418..5131
                     /gene="msyB"
                     /locus_tag="ACHU2L_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1355347.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5280..5846
                     /gene="satP"
                     /locus_tag="ACHU2L_000341"
     CDS             5280..5846
                     /gene="satP"
                     /locus_tag="ACHU2L_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR1355348.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5881..6468)
                     /gene="mog"
                     /locus_tag="ACHU2L_000342"
     CDS             complement(5881..6468)
                     /gene="mog"
                     /locus_tag="ACHU2L_000342"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR1355349.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6583..7536)
                     /gene="tal"
                     /locus_tag="ACHU2L_000343"
     CDS             complement(6583..7536)
                     /gene="tal"
                     /locus_tag="ACHU2L_000343"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1355350.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7815..9245
                     /locus_tag="ACHU2L_000344"
                     /pseudo
     CDS             7815..9245
                     /locus_tag="ACHU2L_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9315..10091
                     /gene="yaaA"
                     /locus_tag="ACHU2L_000345"
     CDS             9315..10091
                     /gene="yaaA"
                     /locus_tag="ACHU2L_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR1355351.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10244..10540)
                     /locus_tag="ACHU2L_000346"
     CDS             complement(10244..10540)
                     /locus_tag="ACHU2L_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR1355352.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10754..12040)
                     /gene="thrC"
                     /locus_tag="ACHU2L_000347"
     CDS             complement(10754..12040)
                     /gene="thrC"
                     /locus_tag="ACHU2L_000347"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR1355353.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(12041..12973)
                     /gene="thrB"
                     /locus_tag="ACHU2L_000348"
     CDS             complement(12041..12973)
                     /gene="thrB"
                     /locus_tag="ACHU2L_000348"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR1355354.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12975..15437)
                     /gene="thrA"
                     /locus_tag="ACHU2L_000349"
     CDS             complement(12975..15437)
                     /gene="thrA"
                     /locus_tag="ACHU2L_000349"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR1355355.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15463..15582)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15518..15583)
                     /gene="thrL"
                     /locus_tag="ACHU2L_000350"
     CDS             complement(15518..15583)
                     /gene="thrL"
                     /locus_tag="ACHU2L_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR1355356.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15797..16483)
                     /locus_tag="ACHU2L_000351"
     CDS             complement(15797..16483)
                     /locus_tag="ACHU2L_000351"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1355357.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16883..17023)
                     /gene="yjjY"
                     /locus_tag="ACHU2L_000352"
     CDS             complement(16883..17023)
                     /gene="yjjY"
                     /locus_tag="ACHU2L_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR1355358.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17119..17835
                     /gene="arcA"
                     /locus_tag="ACHU2L_000353"
     CDS             17119..17835
                     /gene="arcA"
                     /locus_tag="ACHU2L_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR1355359.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17894..19246)
                     /gene="creD"
                     /locus_tag="ACHU2L_000354"
     CDS             complement(17894..19246)
                     /gene="creD"
                     /locus_tag="ACHU2L_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR1355360.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19304..20728)
                     /gene="creC"
                     /locus_tag="ACHU2L_000355"
     CDS             complement(19304..20728)
                     /gene="creC"
                     /locus_tag="ACHU2L_000355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR1355361.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20728..21417)
                     /gene="creB"
                     /locus_tag="ACHU2L_000356"
     CDS             complement(20728..21417)
                     /gene="creB"
                     /locus_tag="ACHU2L_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR1355362.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21430..21903)
                     /gene="creA"
                     /locus_tag="ACHU2L_000357"
     CDS             complement(21430..21903)
                     /gene="creA"
                     /locus_tag="ACHU2L_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR1355363.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22114..22983
                     /gene="robA"
                     /locus_tag="ACHU2L_000358"
     CDS             22114..22983
                     /gene="robA"
                     /locus_tag="ACHU2L_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR1355364.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22980..23627)
                     /gene="gpmB"
                     /locus_tag="ACHU2L_000359"
     CDS             complement(22980..23627)
                     /gene="gpmB"
                     /locus_tag="ACHU2L_000359"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR1355365.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23679..24199
                     /gene="yjjX"
                     /locus_tag="ACHU2L_000360"
                     /pseudo
     CDS             23679..24199
                     /gene="yjjX"
                     /locus_tag="ACHU2L_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(24284..24610)
                     /gene="trpR"
                     /locus_tag="ACHU2L_000361"
     CDS             complement(24284..24610)
                     /gene="trpR"
                     /locus_tag="ACHU2L_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR1355366.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24700..26637)
                     /gene="sltY"
                     /locus_tag="ACHU2L_000362"
     CDS             complement(24700..26637)
                     /gene="sltY"
                     /locus_tag="ACHU2L_000362"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR1355367.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26848..28515
                     /gene="ettA"
                     /locus_tag="ACHU2L_000363"
     CDS             26848..28515
                     /gene="ettA"
                     /locus_tag="ACHU2L_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR1355368.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28622..28789)
                     /gene="nadS"
                     /locus_tag="ACHU2L_000364"
     CDS             complement(28622..28789)
                     /gene="nadS"
                     /locus_tag="ACHU2L_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR1355369.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(28823..30055)
                     /gene="nadR"
                     /locus_tag="ACHU2L_000365"
     CDS             complement(28823..30055)
                     /gene="nadR"
                     /locus_tag="ACHU2L_000365"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR1355370.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(30076..31458)
                     /gene="radA"
                     /locus_tag="ACHU2L_000366"
     CDS             complement(30076..31458)
                     /gene="radA"
                     /locus_tag="ACHU2L_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR1355371.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31507..32475)
                     /gene="serB"
                     /locus_tag="ACHU2L_000367"
     CDS             complement(31507..32475)
                     /gene="serB"
                     /locus_tag="ACHU2L_000367"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR1355372.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32581..33225
                     /locus_tag="ACHU2L_000368"
     CDS             32581..33225
                     /locus_tag="ACHU2L_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR1355373.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33253..34268
                     /gene="lplA"
                     /locus_tag="ACHU2L_000369"
                     /pseudo
     CDS             33253..34268
                     /gene="lplA"
                     /locus_tag="ACHU2L_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            34300..34563
                     /locus_tag="ACHU2L_000370"
     CDS             34300..34563
                     /locus_tag="ACHU2L_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1355374.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(34724..35443)
                     /gene="deoD"
                     /locus_tag="ACHU2L_000371"
     CDS             complement(34724..35443)
                     /gene="deoD"
                     /locus_tag="ACHU2L_000371"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR1355375.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35500..36723)
                     /gene="deoB"
                     /locus_tag="ACHU2L_000372"
     CDS             complement(35500..36723)
                     /gene="deoB"
                     /locus_tag="ACHU2L_000372"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1355376.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36775..38097)
                     /gene="deoA"
                     /locus_tag="ACHU2L_000373"
     CDS             complement(36775..38097)
                     /gene="deoA"
                     /locus_tag="ACHU2L_000373"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR1355377.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(38224..39003)
                     /gene="deoC"
                     /locus_tag="ACHU2L_000374"
     CDS             complement(38224..39003)
                     /gene="deoC"
                     /locus_tag="ACHU2L_000374"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR1355378.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39261..40802
                     /locus_tag="ACHU2L_000375"
     CDS             39261..40802
                     /locus_tag="ACHU2L_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR1355379.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            40774..41637
                     /locus_tag="ACHU2L_000376"
     CDS             40774..41637
                     /locus_tag="ACHU2L_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR1355380.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(41851..42633)
                     /locus_tag="ACHU2L_000377"
     CDS             complement(41851..42633)
                     /locus_tag="ACHU2L_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1355381.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(42630..43703)
                     /locus_tag="ACHU2L_000378"
     CDS             complement(42630..43703)
                     /locus_tag="ACHU2L_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR1355382.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(43825..43986)
                     /locus_tag="ACHU2L_000379"
     CDS             complement(43825..43986)
                     /locus_tag="ACHU2L_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR1355383.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44103..44708)
                     /gene="osmY"
                     /locus_tag="ACHU2L_000380"
     CDS             complement(44103..44708)
                     /gene="osmY"
                     /locus_tag="ACHU2L_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR1355384.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45101..46690)
                     /gene="prfC"
                     /locus_tag="ACHU2L_000381"
     CDS             complement(45101..46690)
                     /gene="prfC"
                     /locus_tag="ACHU2L_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR1355385.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(46781..47458)
                     /gene="yjjG"
                     /locus_tag="ACHU2L_000382"
     CDS             complement(46781..47458)
                     /gene="yjjG"
                     /locus_tag="ACHU2L_000382"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR1355386.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47473..47919)
                     /gene="rimI"
                     /locus_tag="ACHU2L_000383"
     CDS             complement(47473..47919)
                     /gene="rimI"
                     /locus_tag="ACHU2L_000383"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1355387.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(47888..48301)
                     /gene="holD"
                     /locus_tag="ACHU2L_000384"
     CDS             complement(47888..48301)
                     /gene="holD"
                     /locus_tag="ACHU2L_000384"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR1355388.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48404..49435
                     /gene="rsmC"
                     /locus_tag="ACHU2L_000385"
     CDS             48404..49435
                     /gene="rsmC"
                     /locus_tag="ACHU2L_000385"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR1355389.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        11673 a        13518 c        12557 g        11979 t
ORIGIN      
        1 aatgcgaggc gtcttcagga ttagaatcga taaaacaaaa tgggttaaag agagcgatat
       61 aatagcggcg ggtgcttgag gctgtctgtc tcaggcatta gctgaacggc agatagagaa
      121 aagccccgag tgatatttta ccatcaaccc gaggcctcct atatgctgaa cacatgtaga
      181 gtgcctctta ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg
      241 attgtcgccc tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa
      301 gacctctgtg aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac
      361 gaatccgagt aagagcaacg gcggggagtg atccccgcca ctttacaggt gctcgcatat
      421 cttcaacgca ccctattttt acccaggcct gcccacgggc aggcttttgg ggaggttagc
      481 gggtcaggtc gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt
      541 tgtgctcgcc ggttgggcca ccgaagcttt cttgcagctc ttgcagcagc tgtttctgct
      601 tttcgttcag gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcgc
      661 caccgcggac agacttgacg cctttaccgc gcatacggaa tagcttaccg gtctgggttt
      721 cgccaggcac tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca
      781 gcgccgccat agcgaagttg atcgggactt cgcaatacag gttgttgcct tcacgctcga
      841 aaatcgggtg ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct
      901 cgcccgcttc accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga
      961 ttttaacgga cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg
     1021 gatctttgat cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaga
     1081 atccctggcg catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg
     1141 tacctggttt tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga
     1201 tctctttggt cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat
     1261 cagcaccgcg cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt
     1321 caccaaaaat atcgctgaag tctgcgccgc cgccaaaacc accgccgccc atgcctcctt
     1381 gctcaaacgc agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa
     1441 cttcataagc ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac
     1501 ggtccgggtg gtatttcatg gccaggcgtt tgtaggcctt tctgatttca cgctcttccg
     1561 ctgttttgga aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgc
     1621 ccctagatga atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc
     1681 cgtttatagg gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg
     1741 tcatctttcg cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc
     1801 tgctgggcga tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg
     1861 atagcggctt tgtcttcacc tttcagagca gtttccagtg cagtcagcgc agactcgata
     1921 gcagttttgt cgtcagccgg cagtttgtcg cctgcttctt caacctgctt acgggtgctg
     1981 tgcagcagat ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt acggtcagct
     2041 tcggcgttag cttctgcgtc gcgtaccatt ttctggattt catcttcgtt cagaccagaa
     2101 gaagccttga tggtgatctt ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc
     2161 aggataccgt cagcatcgat atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc
     2221 gggttgatac catctaggtt gaactgaccc agagatttgt tatcagccgc acgtttacgt
     2281 tcaccctgca gcacatggat ggttaccgca gactggttgt cttcagcggt agagaacacc
     2341 tggctgtgct tggtcgggat agtggtgttt ttcgcgatca gcgtcgtcat cacaccgccc
     2401 atggtttcga tacccagaga cagcggggta acgtccagca gcagtacgtc ttttacgtca
     2461 ccagtcagaa caccaccctg aacagcagca ccgattgcta cagcttcgtc cgggttaacg
     2521 tctttacgcg gctctttacc aaagaactca gcaactttct tctgaaccat tggcatacga
     2581 gtctgaccac caacgaggat aacgtcgtcg atatcagata cggacaggcc agcatcctgc
     2641 agtgcaactt tcagcggctc aatggaacgg tttaccagat cttcaaccag gctttccagt
     2701 ttcgcacgag tcactttgat gttcatgtgt ttcggaccgg tcgcgtctgc agtgatgtat
     2761 ggcaggttaa cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt ttctgccgct
     2821 tctttcaggc gctgcattgc cagcggatcg ttgcgcaggt caatgccctg atctttcttg
     2881 aattcttcaa ccagatagtt gatcagacgg ctgtcgaagt cttcaccacc caggtgggta
     2941 tcaccgttgg ttgccagaac ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata
     3001 atagaaatat cgaaagtacc accccccagg tcataaaccg cgatagtacg gttgccagtg
     3061 cctttgtcca gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat acgttttact
     3121 tccagaccag cgatacggcc tgcgtctttg gttgcctgac gctgagcatc gttaaagtat
     3181 gccggtacgg tgataacagc ttcagttacc ggttcaccca ggtaatcttc agcggttttc
     3241 ttcatttttt tcagcacttc agcagaaatc tgcggcggtg ccattttctg gcctttaact
     3301 tcgacccatg cgtcgccgtt atcagcagca ataattttga acggcatgat ggaaacatca
     3361 cgctgtactt cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg
     3421 ttttgcgggt tcgtcactgc ctgacgttta gccggctgac caaccagagt ttcaccatcc
     3481 tgggtatagg caatgataga aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga
     3541 ggaatggtgc catccataat cgctacacaa gagttggtag tacccaggtc gataccaatt
     3601 attttaccca tctaaacgtc tccactatat attcggtcat catgtggttg tgagtctgta
     3661 ataggggcga aacgtctggt ttcaactgcc caatttcatt tttttgcaga ctcactcact
     3721 gcggttgact actaaatggg gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt
     3781 ttgtgctttt gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat
     3841 tttattcata taaagaatga atcgcagcca aggcgttcat caaaaaattg taataaaaag
     3901 aaaagattac gtgcctgaat cttctcttta tcagcagtaa actagtgggt attcatcccc
     3961 ctacctcttc ccactaagag aatccttatg aaatccgttt ttacgatttc cgccagcctg
     4021 gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg accataaact cctcggcgtc
     4081 attgcaatgc cgcgtaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt
     4141 gtgaaacgca tacaactctc tgccgaccat ggcgatttac agttaagtgg tgcatcggtt
     4201 tatttcaaag ccgcccgtag cgccagtcag agcctgaata ttccttcaga aataaaagaa
     4261 gggcaaacca ctgactggat caacattaac agcgataacg acaataaacg ctgcgtctca
     4321 aaaatcacct tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc
     4381 ggcgacgact aacgccagaa tatagaagcc acaaaaaatg aatgttaatt acctgaatga
     4441 ttcagatctg gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt
     4501 gctcacccat aatgaaaaag gcaaaactcg cctctccagc atactgatgc gcaacgaact
     4561 gtttaaatcg atggaagggc atcccgagca acatcgccgc aactggcagc tgattgccgg
     4621 agaattacag cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt
     4681 gtatcgggcc attttgctcg atgtttcaaa gcgattgaag ctgaaagccg acaaagagat
     4741 gtctacgttt gaaattgagc aacagttact ggaacaattt ctgcgtaata cctggaagaa
     4801 aatggacgag gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct
     4861 ggaagagctg ctgccgctgc tgatgaaaga caaattattg gcaaaaggtg tgtcgcattt
     4921 gctttccagc caactgaccc gcattttacg cacccacgca gcaatgagcg tacttgggca
     4981 tggtttgctg cgcggcgcgg ggctgggagg ccctgtgggc gcagcattaa atggggttaa
     5041 agcggtcagc ggcagcacct atcgcgtgac gattccagcc gtactgcaaa tcgcctgcct
     5101 gcgccggatg gttagcgcca ctcaggtctg ataccggaaa ccggggaaaa ttttcatttt
     5161 cccctcaaaa gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac
     5221 actacagtta ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta
     5281 tgggcaacac taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca
     5341 ccattctgct taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca
     5401 tgggcatttt ctacggcggc atcgcgcaaa tttttgctgg tctgctggag tacaaaaaag
     5461 gcaacacttt cggtttaacc gcattcacct cttacggttc tttctggctg acgctggttg
     5521 cgattctgct gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg
     5581 tctacctggg tctgtggggc gtatttacgc tgtttatgtt cttcggcact ctgaaaggcg
     5641 cgcgcgtcct gcaattcgtt ttctttagcc tgaccgtgct gtttgccctg ctggcgatcg
     5701 gtaacattgc cggtaatgcc gcgatcatcc actttgccgg ctggattggt ctgatctgcg
     5761 gtgccagcgc aatctatctg gcgatgggtg aagtgctgaa cgagcagttt ggtcgcaccg
     5821 ttctgccgat tggtgaatcc cactaattgt cttgagatcc ccccgctcgg ggggattttt
     5881 ttattcgcta acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc
     5941 cgtttcaacg tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc
     6001 atgcaccaca acgttaccct cagcgtcctt cacaccttcc agcgtctctt taatagactt
     6061 cggctgaccg ggtaagttaa ggatcagcgc ctgtttgcga atcacgccca cctgacgcga
     6121 aaggatcgca gttggtacaa aatgcaggct gatctggcgc atctgttcac caaagccagg
     6181 catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc
     6241 agttccgccc gtggtgagca ccagatggca actcatttca tccaccagct cacacaacgt
     6301 ttgctcgatg atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt
     6361 tagcgccgat gtcagccatt cttccagcgc tggaatgcct ttatcctgat aaacgccgct
     6421 ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc
     6481 gtttaaacaa tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca
     6541 gaagaggtag cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat
     6601 cattttttcc agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc
     6661 tactgccatt ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg
     6721 acgcgctttc acttcgccgg tgtaagacag tttacgttcg atagcccctt cgctctccgc
     6781 cagctctttc agcagtgccg gtgcgatggt cagacggtca cagcctgcca gttccagaat
     6841 ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta
     6901 gtactggtag atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt
     6961 cttatcggta ttcgctttgt accagtcaag aatacggcca acaaacggcg agatcaggaa
     7021 cacgcccgcc tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca
     7081 gttgatacct tctttttcca gctgttctgc agcacggata ccctgccagg tagaagccag
     7141 tttgatcaga atacgatcgt tgctaatacc tgcatcgttg tagagtttga tcaggcgttt
     7201 tgctttcgca attgacgctt cagtgtcata ggaaagacgc gcatcaactt cagttgagat
     7261 acggcccgga accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc
     7321 gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc
     7381 aatcaactta cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt
     7441 ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt
     7501 ggtgtactga cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct
     7561 tgttaggggg atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg
     7621 ccgacaagac ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc
     7681 gcccaccatt tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta
     7741 gcgtatatac ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa
     7801 taagagggat atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg
     7861 taatgattta cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc
     7921 agtttcgcta catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag
     7981 gcggtttaac ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca
     8041 acctggccgg cgttgcgctg gcaattaccg ccggtggacc aggtgccgtc ttttggatgt
     8101 gggttgctgc gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt
     8161 ataaagaacg tgacgtcaat gggcagtttc gtggcggacc ggcatggtat atggcgcgcg
     8221 ggctggggat gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca
     8281 taattttcag cggagttcag gcgaacgccg ttgcccgagc cctgagtttt tcttttgatt
     8341 ttcctccgct ggtgacgggc attattctcg ctgtctttgc tctgctggca atcactcgtg
     8401 gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg ataatctggg
     8461 tactgaccag cctggtgatt tgcgtaataa atatcgggca acttccccac gtcatttggt
     8521 ctatttttga gagtgctttt ggctggcagg aagcggcagg cggcgcggcg ggatatacct
     8581 taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag gtgggaatgg
     8641 gatcgacgcc aaacgcggca gcggcagctg catcctggcc tccgcatccg gcagcacaag
     8701 ggattgtcca gatgattggc atttttatcg acaccctggt catctgtacg gcaagcgcca
     8761 tgctgatatt actggcgggt aacggcacaa cctacatgcc gctggaaggt attcagctta
     8821 tccagaaggc gatgcgggtg ctaatgggtt cctagggtgc tgaatttgtt accctcgtgg
     8881 ttattctgtt tgccttcagc tccatcgttg ccaactacat ttatgccgaa aacaatctct
     8941 tctttttacg cctgaacaac cctaaagcga tctggtgttt gcggatctgc accttcgcaa
     9001 cggtcatcgg cggcaccttg ctaagccttc cgctgatgtg gcaactggca gatatcataa
     9061 tggcctgcat ggctattacc aatttgaccg ccattttact gctctcgcct gtggttcata
     9121 ccattgccag tgattatcta cgccagcgta aactcggcgt gcgcccggtg tttgatccgt
     9181 tgcgttatcc ggatatcggt cgccagcttt ctccggacgc gtgggatgat gtttcgcagg
     9241 agtaatcaca actatcgatc aactcattct ctttttttgc taaagtcggc ataaatttcc
     9301 tgcaaggact ggatatgctg attcttattt cacctgcgaa aacgcttgat taccaaagcc
     9361 cgttgaccac cacgcgctat acgctgccgg agctgttaga caattcccag cagttgatcc
     9421 atgaagcgcg gaaactgacg cctccgcaga ttagcacgct gatgcgcatc agcgacaaac
     9481 tggcgggtat caacgccgct cgctttcatg actggcagcc agatttcacg ccggcgaatg
     9541 cccgccaggc gattctggcg tttaaaggtg atgtctacac cggcttgcag gccgaaacct
     9601 tcagcgaaga cgatttcgat tttgcccaac agcatttgcg aatgctttcc ggcttgtatg
     9661 gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct ggaaatgggg atccgtcttg
     9721 agaatgcccg agggaaagat ctgtatcaat tctggggaga tatcatcacc aacaagctga
     9781 acgaggcgct cgcagcacaa ggcgataatg tggtgattaa tctggcgtca gatgagtatt
     9841 ttaaatcggt gaagccgaag aaattgaatg ccgagattat caagccggtg ttcctcgatg
     9901 agaagaacgg caaatttaag atcatcagct tctacgctaa gaaagcacgc ggtctgatga
     9961 gtcgtttcat tattgaaaat cggctgacca aaccagagca actgactggt tttaatagcg
    10021 aaggttactt ctttgatgaa gattcctcca gcaatggcga actggtgttt aaacgctacg
    10081 agcagcgtta agtttgtagt caatgccgga tgcggcgtga acgccttatc cggcctacaa
    10141 aagcatgcag attcaatata ttgcagaatt aacgtaggcc tgataagacg cgccagcgtc
    10201 gcatcaggcg ctgaatgccg gattgttacc cggcatttgt catttagcgg tgatgtttgc
    10261 ctggaccatg accgccgtga tgatcatgag gagctttctt atggtggcgc ggcggtggcg
    10321 gcggtccgtg tgggtgccag cgattgcctc gccattcata atgttgtttc caccagccgt
    10381 ggtcgcgcca gtgacctcca tcccagtaat agccacgatt atcacgatcg cctatctgta
    10441 attttactga cgggactaac gtaatttccg cagcctgtgc tgccatggga gcgaccagaa
    10501 ccagggaaag tgcgagtacg atagattgca tctttttcac aggttactcc ttttatcatt
    10561 gcccgttatg ggtcgatatc cggagattac gcgatgagaa cgacggttgt tattctccgc
    10621 aatcctaatc tctaagttac cgcatttatt gataatttct cctttttccc tgtcgttttt
    10681 ctccataatt tcttcataaa aaagccgggc tgcataaaag caaacccggc ctgattgaga
    10741 taatgaatag attttactga tgattcatca tcaatttacg caacgcagaa aaatcggcgg
    10801 gcagattatg tgaaagcaag ggtaaatcag cacgttctgc cagctctttt ggcagatcca
    10861 acgtttcacc gagaatcgct tccacgctct ctttaaattt cgccggatgc gcggtgccga
    10921 ggaacaagcc atattcgcct ggattcaact ggtcacgcag cgcacgatac gctacggcag
    10981 cgtgcggctc cgaggtgtag cccagttctt ttaactcacg cattgtctgt tgcgtggttt
    11041 catcatccac ggctgcataa cccagctctt tcagttgcca gattttgcgg cggaacaact
    11101 cttccacacg cggccagttg ttcggctgac tcacgtccat cgcgttggat aacgtcgcct
    11161 gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg tggcacggta tcgttcacgt
    11221 tggtcgcagc aataaaacgt ttcaccggca gaccgaatga cttcgccagc agacccgccg
    11281 tcaaatcgcc gaagtttccg cttggcaccg agacaaccag ctggttgcgc gcctcctgcg
    11341 gcagctgcgc aacagcttca aagtagtagc aaatctgcgc cagcaaacgg ctgatgttaa
    11401 tcgagttagc cgagtttaac cccagcgcca ctttcagttc ttcatcatca aacgcctgct
    11461 tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc
    11521 ccaacgtaca gaacagtttt tcttgcagtg gactgatttt gcctcgtgga tagaggataa
    11581 ccactttcac attcggcaaa ccgtagaaag catgagccac tgcagctccg gtatcaccgg
    11641 aggtcgcggt cagaatggtc actggcttat catccgcaat atgggtcagc atttgtgcca
    11701 taaagcgacc gccgaaatct ttaaatgcca gcgttggccc gtggaacaat tccagacaac
    11761 cgacatcgct ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct
    11821 cttccaggat ttcctgtggg atttcatcac caataaacgc cgagaggatc ttcgcactgc
    11881 gggtgacaaa atccagcttc agcatctcat caatttcagt caggctgaat tccggcaggt
    11941 cgtgcggaaa aaacagcccc tgatttttgc ccaacccctg ggttacggct tgcgcaaagc
    12001 tgacctgctc gttgtgatct ttcagattgt agagtttcat ttagttttcc agtactcgtg
    12061 cgcccgccgt atccagccgg caaatatgaa caaaaccttc ctgattttgc aggtagttct
    12121 tacccaacca gtcggcaacg cgctgggcgg tatccggctt gtcacataga gcgaacaagg
    12181 tcgggccgga gccggagata ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg
    12241 cctgccggaa gccaggcagt aaccgttcac ggtagggttc agcgataaca tctttcatca
    12301 gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg aatgaagcca gccagatgtc
    12361 gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg taaaatagcc cgggcttctg
    12421 ccgtcgagac tttaattccc ggatacgcca gcacccacag ccactcatca aaccctggca
    12481 cttgctggct gatgatgtcg ttttcttcga tcatcaactg cataccacca agaaaacacg
    12541 gtgccacgtt gtcgtaatga atgctgccgg agatacgtcc ttccagctcg cccatcaaag
    12601 ccagcaaacg agtgtcatta agcggcttgc cgcagtgttc attcatcgcc atcagcgccg
    12661 cgaccaccga acaggcgctg gagcctaagc ccgaaccgat cggcatattc ttttccagag
    12721 tcatcgccac tggaatttgc ttgccaagct cctggcaaaa acgctcccag cactgataaa
    12781 cgatattttc ccgtggttct gacggcagct tatcggcaaa gcgtccgagt ttgttgagac
    12841 tgaatgtctc tgccgcctca accgtgacta catctccgag caatgcacca tcaacgggtg
    12901 ccaccgccgc cccgagcaca tcaaacccga cgctcatatt ggcactggaa gccggggcat
    12961 aaactttaac catgtcagac tcctaacttc catgagaggg tacgtagcag atcggcaaag
    13021 acaccggcag cggtaacgtc attgcccgca ccatatccgc gcagcaccaa cggcagcggc
    13081 tgataatagt ggctataaaa ggccagggcg ttttcgccat ttttcacttt gaacagcgga
    13141 tcattcccat ccacttcggc aatcttcacg cggcagacac catcttcatc aatattgcca
    13201 acatagcgca aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc aaagagatcg
    13261 tcgagctgtg acagattcgc cataaaagcg gcaacatcac cctcggcgtt aaactctgcg
    13321 ggcagcacag gttcaatttc aatatccgcc agctccagtt cacgtcccgt ttcacgagcg
    13381 agaatcaata gtttacgcgc cacatccata ccagaaagat catctcgcgg gtccggttcg
    13441 gtataaccca tttcccgcgc cagcgtggtc gcctcggaga aactcatgcc ttcgtctaac
    13501 ttgccgaaga tataagaaag cgaacctgaa agaatgccgg agaacttcac caattcatca
    13561 cctgcattga gcagattttg caggttctca ataaccggta atccagcccc aacgttggtg
    13621 tcatagagga atttacgccg cgatttttcc gccgcataac gcaactgatg atagtaatcc
    13681 atcgacgagg tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc gcgcaggaag
    13741 tcggcatatt gatccgccac tgcctggctg gaggtgcagt caacaatgac cgggttcagc
    13801 agatgatatt ctttcacgag gcgaattaag cgcccgagat taaacggctc tttggcttgt
    13861 gcaagttctt cctgccagtt ttccagatta aggccatgca cactagtgag cagagccttc
    13921 gagttggcaa cgccgcagac acgtaagtcg atatgtttat tcttcagcca gctttgctga
    13981 cgcttcagtt gctccagcag cgcaccgcca acgccaccga cgccaatcac aaacacttcg
    14041 ataacctgat cggtattgaa cagcatctga tgagtaacgc gcacgccagt ggtcgcatca
    14101 tcgttattta ccacgacaga gattgagcgt tcagaagatc cctgagcaat ggcgacaatg
    14161 ttgatattgg cgcgggccag cgcggcaaag aatttcgccg agatcccacg caaggtgcgc
    14221 ataccatcac ctaccaccga gataatggcc agccgttccg tcaccgccag cggctccagt
    14281 aagccttctt tcagttccag gtagaactct tcctgcattg cccgttcagc tcgcacacag
    14341 tcgctttgtg gaacgcagaa actgatactg tattcggaag atgattgcgt aatcagcacc
    14401 acggaaatac gggcgcgtga cattgcagca aagacgcgcg ccgccatgcc aaccatccct
    14461 ttcatccccg ggccggaaac gctgaacatt gccatgttat tcagattgga aatgcccttg
    14521 accggtaatt cgtcttcatc acggctggca ccaatgagcg tacctggtgc ttgaggattt
    14581 ccggtatttt taatcaggca agggatctgg aactgggcga tgggggtaat ggtgcggggg
    14641 tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt cctggtagga catcgacttc
    14701 aacaacctcg catcgggcac ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc
    14761 caaatctcgc aacaatcggc gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa
    14821 ccgttacgtc caagcaccac cagttcgcct ttttcattac cggcggtgaa acctgccatc
    14881 aacaccatgt gatcagccgg aatgcggctt gccgcaatac ggcgggtgga ctcagcaata
    14941 tcgacggtag attcgaggta atgccccact gccagcagtt tttcgaccgg atcgataacg
    15001 gtaacgttgt gaccacgcgc ttctaacacg ccggccataa tggcgatcga cattttctcg
    15061 ccacggcaaa tcagcgcagc gttgatgcta tccgggcact gccccaacaa actaatgcca
    15121 tgcaggacat gttttatttg ggcaaattcc tgatcgacga aagttttcaa ttgcgccagc
    15181 gggaaccccg gctgggcggc ggcgagtccc gtcaaaagtt cggcaaaaat acgttcggca
    15241 tcgctgatat tgggtaaagc atcctggccg ctaatggttt tttcaatcat cgccaccagg
    15301 tggttggtga ttttggcggg ggcagagagg acggtggcca cctgcccctg cctggcattg
    15361 ctttccagaa tatcggcaac acgcagaaaa cgttctgcat ttgccactga tgtaccgccg
    15421 aacttcaaca ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa agcccgcact
    15481 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
    15541 taatggtgat ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg
    15601 taatttttat ctgtttgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
    15661 aataaaattt taatttactc acggcaggta accagttcag aagctgctat cagacactct
    15721 ttttttaatc cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata
    15781 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
    15841 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt
    15901 ttatgtcatc tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt
    15961 gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta
    16021 attgatagca atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca
    16081 tcggcacacc agtaagcacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt
    16141 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
    16201 ccagttctac tggcgtggcg tagtaatgat atttcgcccg actacgcgca gtggtggcga
    16261 cagtgaaatc gacatcgtgt aacgattcag ccagtgtcgg gaaaacttta atattatcaa
    16321 taatatcacc agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat
    16381 cgacaatccg cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat
    16441 tttctgctct ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga
    16501 ccaaataacc gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat
    16561 tcttaacgta agccgcagaa aaagcccttt acttagctta aaaaaggcta aactattttc
    16621 tgactgcact aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat
    16681 aattttataa aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc
    16741 aactagttgg attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat
    16801 caaactgtta acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg
    16861 gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt
    16921 ttgacactgt cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt
    16981 tgatggaaag tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt
    17041 aatttgcagc atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg
    17101 gcaatttagg tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta
    17161 acacgcaaca cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca
    17221 gatggcgcgg aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat
    17281 atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat
    17341 gttgcgttga tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa
    17401 atcggtgcag atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca
    17461 cgcaacctgc tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt
    17521 gaaagctaca agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct
    17581 gatggcgagc agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa
    17641 aacccaggca aaattcagtc tcgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg
    17701 aaaccgcacg accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct
    17761 acgccggata cgccggaaat catcgccacc attcacggtg aaggttatcg cttctgcggt
    17821 gatctggaag attaatcggc tttaccaccg tcaaaaaaac ggcgcttttt agcgccgttt
    17881 ttatttttca accttatttc cagatacgta attcatcgtc cgttgtaact tctttactgg
    17941 ctttcatttt cggcagtgaa aacgcatacc agtctatatt acgagtcaca aacatcatgc
    18001 cggccagcgc caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga
    18061 gcagtcccca catcactcca tccagcaaca acagcgcgag ggtaaacaac atgctattgc
    18121 gccagccttt caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg
    18181 cgattatcca tgccacggta aaaccgatat gttcagaaag cgccagcaag agcaaataaa
    18241 acatcaccaa tgaaagcccc accagcaaat attgcattgg gtgtaaacgt tgcgcggtga
    18301 gcgtttcaaa aacaaagaac gccataaaag tcagtgcaat cagcagaatg gcgtacttag
    18361 tcgcccggtc cgttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg
    18421 ggaagttttc ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa
    18481 accagctgct ttgccattgc gcctgaaaac ctgactcgct agcttcccgt ttggctggta
    18541 gaaaatcacc taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac
    18601 gcccggcagg caccacagaa agatcgccgg taccgcttaa attcagtgcc atattcagct
    18661 gcaggttctg cttccgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt
    18721 gctctaaccc ggtaccgggt tcaatggtca gtgccgttcc gttaacttca ggcgctttca
    18781 ccacaccaat accacgcgca tccccgacgc taatcacaat aaagggcttg cctaagatga
    18841 tatttggcgc gttgagctcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat
    18901 cactgtgcca gacctgaccg gtataaatcc ctatcttgcg ttcttccacg ttctgattgc
    18961 catcaaccat caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg
    19021 ttttatcctc ttcctgcacc gtataaagct cggtcacagg aatagcgata agcggcccaa
    19081 cgagtttttg cggcccgctg gtactttggc gaatcgcatc ttccacatcg ctacggtaat
    19141 cggcacgttc gacaatcact tgccgaatca gcattatcgg aatcaacaac agcaatactg
    19201 caccaaacaa ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg
    19261 caatggcagc aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt
    19321 gaagtcgtag cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt
    19381 taaacaaacg ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat
    19441 ttgcacgcgg caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac
    19501 cactaccggt atccagcacc ttaagcgtga cgtgttcctg atccacttcg gcgcttagcg
    19561 ttatgcaacc gctctcggga gtaaaatcga tggcgttatc cagtaaattc cccagcgcct
    19621 gatccagtaa cgccggttca gcagcaacat taacctcggt gggcataaca tgcaaagtga
    19681 tgtttttttc tgccaactgc acggtgcgcg cttcgctgac gcggcgaaat aatgccgcca
    19741 catcaacagc agtcagaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg
    19801 tttccaccag cgcctgcatt cgcgcatttt gtgtcagaat gttgtcagta aaacgagcca
    19861 ctacttcagg cggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg
    19921 ggctttttag ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca
    19981 gcttcacgcg catactttcc agcgcctgcg cgagtttacg cagctcgcta ctaccgagat
    20041 cgggaagagg aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa
    20101 tggagcggtt gatccaccaa accatgcctg cgccaatcac cagtgcaatc cccaacaaaa
    20161 tggcgctggc ccataaaatt cgccgctcgc tgcgtttaat gaccggagcc atcgccgcgt
    20221 tcggtttgcc tacgctcaaa acgccaataa gccgcgagcc gcccataatc ggcgcggcaa
    20281 catacatcac cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact
    20341 gaccacgcaa cgttagccag acgtcattcc agcgcgaata atcctgtcca acggctttat
    20401 ttgccgaatc gaacaatact ttgccatgcg catcggtcat atagacatga tattcattgc
    20461 gcactttgtt aatgccacca atattggcgc gaaacgggcg atgttgtagc tgattaaacg
    20521 cctgcgccag ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg
    20581 caagtaacgt tgccgtgtcg atcaacgtcc cctccgttgc tcttcgcacg cccggtttaa
    20641 cttctttgac aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc
    20701 ccagcagcaa ccgcatgccg atacgcatta caagcccctc aggctatatc ccatgccgcg
    20761 atgagtatta atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcagcgtttt
    20821 aatgtggata tcgacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat
    20881 tagttgctgg cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa
    20941 ctcataccgc gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt
    21001 caattcaaaa tgaccaatac ggatgacggg agacggcgtc gagaacttct tcacccgacg
    21061 cagtaaggtg cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc
    21121 gtcagcgcca atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa
    21181 cagtacaggt aacgccggat agagcgccag taactggcgg cacaattcaa agccactgat
    21241 atcaggcaaa ccaacgtcga gaatcatgac gtcgggaacc tgctggcgag ctttatccag
    21301 caccggcaaa cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac
    21361 cagcgtgtcg gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa
    21421 tcgcctctgt tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga
    21481 accttctaca actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tggcgtcata
    21541 aaagcgcacg acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc
    21601 gccctgagct ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca
    21661 agaaatggcc gcatcggagg tatcttctgc caggcccaat ccccctttaa taccaccggt
    21721 tttcgcccgg ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac
    21781 aacaattttg tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc
    21841 tgcatgagcc aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt
    21901 catattgtta ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata
    21961 ttgattaaga acaaaatctc aattctttta tttccgtcaa gccctaaaac atactctact
    22021 aagaaaaaaa cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc
    22081 aggtgctcgt tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac
    22141 cttttaatct ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg
    22201 aaagcaggtt attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct
    22261 attggcgcgt atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact
    22321 gcgcgtccga ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc
    22381 cgcgcattca agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg
    22441 agcgcctttg gtattcgccc gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa
    22501 tttgtcaccc tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg
    22561 gagcaaatct ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc
    22621 aacgcgccga ccattccgcc ggtgctctac ggcctgaatg aaacgcgtcc gagtcaggat
    22681 aaagacgacg aacaagaggt attctatacc accgcgttag cccaggatca ggcagatggc
    22741 tatgtactga cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat
    22801 gaaggtctgg gaaccggcgt gcaggagttt atcctgacgg tatacggaac gtgcatgcca
    22861 atgctcaatc tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagac
    22921 gccaaagcgg gagatcgccc aattaatctg cgctgtgaac tgctgattcc tatccgccgt
    22981 taacgctgca gctcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca
    23041 acgacccagc cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag
    23101 ttacgcagac gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccaga
    23161 catcccagtg caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg
    23221 caagattcca gtgccgcatt gacgcgatcg ctgagctcct gcattgactc gccttcagga
    23281 atacgcccgt caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtc
    23341 agagaatcga tatgtctttt ttccagcaca cccatgttta attcacgcag gcgagaatca
    23401 aagatgatgt cacagccgca ggcctgggcg atgatttccg ccgtacgccg ggtgcgtcct
    23461 aaatcgctac tgataatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc
    23521 gcctgttgct cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg
    23581 gcgttccact gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt
    23641 atactgctgt aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga
    23701 ccaccaatcc cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag
    23761 gatcctgcca tattgcatcc gttgccgtcg agagcggtgt accggaacaa ccctttggca
    23821 gtgaggaaac gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag
    23881 aggctgattt ttgggtggcg attgaagctg gcattgatgg cgacagcact ttcagttggg
    23941 tggtgattga aaacgccagc cagcgcggcg aggcgcgttc ggcaactttg ccgttacctg
    24001 cggtgattct ggaaaaagtt cgtgaaggcg aggcgcttgg tcccgtgagt cgcgttatac
    24061 cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact
    24121 cactcgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc
    24181 cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccgaca ttcggtgcac
    24241 gatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca
    24301 acacctcttc cagccactga cgcagctcca cgggcgcggc tttcaggctg ttagatccac
    24361 gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc
    24421 cacgcaacag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg
    24481 tcagcatcag gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt
    24541 cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt
    24601 gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgactcc cccggttgta
    24661 ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc
    24721 cgtggcgctc atcaacgtcg gtttatcccc catgaaatag cggtagtaag catcataagc
    24781 cagcacgttc ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac
    24841 agcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt
    24901 ataagctgct gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta
    24961 actggtgcca atgttgatgt tcgtttccgg atccagcaat tgcccagggc tgctataacc
    25021 aggaatagag aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc
    25081 gctggcccct accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc
    25141 catcgcatag ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta
    25201 agccagcggg aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac
    25261 gctaagatcc caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt
    25321 tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtattatcga gattccagta
    25381 catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggtgctgt caacattctg
    25441 cggcgcttta tcaatcttca gctcatactc ttcaccgatg cgttgtgctg caaccatcgg
    25501 gtagaaacca cgctgttgta tgagttgatg caaaatctct ttcgcttcag cttcacgtcc
    25561 gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt ttgcttccat
    25621 cggcagacgc gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gcgccattcg
    25681 cacacggcgt tcaatcaacg acgtggattg cgagcgcata atggcgtcat cgcgccattt
    25741 cgcctgctca tcggtgacat cattgcccat caaacgccag gcgacgatat cgcgcagctc
    25801 ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc
    25861 attttcggca tcctgccgcg ccacactggc aaacgccacc gccgccattt gacgggtaaa
    25921 atcggtcgcg ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga
    25981 aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac
    26041 caggcctgtg ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc
    26101 ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt
    26161 gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca
    26221 tttcgcatag tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc
    26281 taacaagcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt
    26341 gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg
    26401 attcatcagg tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt
    26461 cagtccaggc atcatttgtt cgaccacatc catctgtcga ttatcccagg cctgcttgat
    26521 ttgcgcgtaa cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt
    26581 cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag
    26641 cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta
    26701 accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga
    26761 tcgaccaact gggattaggc ggggaaacag gctacacttc gcctttgaaa actcatcatc
    26821 acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc
    26881 ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct
    26941 ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtccaccct gctgcgcatt
    27001 atggcgggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt
    27061 ggttatctgc cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa
    27121 gaagcggttt cagaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac
    27181 gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc
    27241 attcaggctc acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgctg
    27301 cgtctgccag attgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgtg
    27361 gcgttgtgcc gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac
    27421 cacctggatg ccgaatccgt ggcgtggctg gaacgcttcc tgcacgactt cgaaggcacc
    27481 gtggtggcga ttactcacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa
    27541 ctggaccgcg gtgaaggaat tccgtgggaa ggtaactact cctcctggct ggagcagaaa
    27601 gatcagcgtc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa
    27661 gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg
    27721 gcacgctttg aagagctgaa cagcaccgaa taccagaaac gtaacgaaac caacgaactg
    27781 tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa
    27841 tcctacggcg atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcgatc
    27901 gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctccggt
    27961 caggaacagc cggacagcgg caccatcact ttgggtgaaa cggtgaaact ggcatcggtt
    28021 gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg
    28081 ctggatatta tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt
    28141 aactttaaag gggttgatca gggtaaacgt gttggcgaac tttccggtgg tgagcgcggt
    28201 cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca
    28261 accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg
    28321 ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc
    28381 ctggactacc aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa
    28441 gagtacaaaa aacgcacgct gggcgcagac gcgctggagc cgaagcgtat caagtacaag
    28501 cgtattgcga agtaatgcgt aaaatgccgg atgcggcgcg aacgccttat ccggcctaca
    28561 aaaccagcaa tttcaatacg ttgtaaaact gtaggcctga taagacgcgg taagcgtcgc
    28621 atcaggcatt gagcaccgaa tgccggatgc ggcgtgaaca ccttttccgg tctacaacag
    28681 ccttagcatc ctcaatttcg tggcgggtca cccgtgcagg ttttttcaaa ccttgtttaa
    28741 tctcgacagc ctcttccaga gaggttttca actcatcaaa aaaactcatt ttatacctcg
    28801 cctttgagca gtttcgtcac ggttatcctt gctcccccat catctcccgc accagctcca
    28861 cgcagcgcag gaaacggctg tcgtaatcgt cctcttcaac ccgcacgaat tcgatattgt
    28921 tctcttccag catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga
    28981 ggctgcgtaa accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga
    29041 aacggtattc atcaatcagc gcctgcacga acggatgctc acgcccttcg tactttttgc
    29101 agaacgcctg ggtagtgaca aaatcggtgt caataaacgc cactttattg gcgtatttca
    29161 ctgcaaaatc aatgtattgt gcgtgaccca acgcgatttt atcgtagtcg gaatactgca
    29221 atgcgatttc atcaccgccg aggtgtgaaa agacataatc gcggccatat tcccacgcac
    29281 tggtggtgtt gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc
    29341 cgccaaggat cgccacggta cgcacaaaaa acggcttcac ttcggtagga atatattccc
    29401 agtagcggaa cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg
    29461 gatcgaccag caccgtctcg atccccagat gttccatata ctgtggcgca tcggcttctt
    29521 ccgaggtgta gatcaggtcc ggctggatcc ccttttcagc cataaacttt ttgatgccgt
    29581 tgctccacac atcccagcca tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa
    29641 tacggatatt tttctgatat ttaaaggttt gcaatagcca gcgcagacga tccggcaccg
    29701 tgggctgctg cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac
    29761 ccataatgat atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc
    29821 cggtatgcag cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgccggggaa
    29881 attcaagccc caaaaaacgg tgcaatgcct ccagcttttg cgcgctgggg cttttaattt
    29941 tggcattcag taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct
    30001 gtagcgtgca gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc
    30061 cctccgtatc tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac
    30121 gccaaaaatc tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc
    30181 ccggcgaaaa ccgtgtttcg ccgcttcaga aatccgctcc tggccactgg gcaccgggcg
    30241 gatctcccct gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc
    30301 acgcaggctg gaaaccatcg ccagcagtaa cgctaagtcg gcgctggttt cggttacctt
    30361 cacgccgccg accacgttca caaacacatc ctgatcggcc atttgcagac caccgtgacg
    30421 gtgcaagaca gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg
    30481 cggattcgcc attatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt
    30541 tccttcccac accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa
    30601 aattgccgaa gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag
    30661 ctcattcacc gcgccgaagc ggtttttatg gctgcgtaag gtgcgaaaac gggagtcggc
    30721 atcgccatcc agaagcaccg aacagtcgat acagtgttcc agcactttcg ggccagccag
    30781 cgaaccatct ttggttacgt gtccaaccat gacaatcgcc acaccgcgcg ttttggcgaa
    30841 acgcgtcaga taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac
    30901 atccgccata tgcatcacct ggatcgagtc aattaccatc agcttcggtt gctcttcttc
    30961 ggcaatcagg cagatctgct cgatgctggt ttccgacaac atattgagat tgtcagtcgg
    31021 caggccaagg cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata
    31081 cagcgttttc atctgctggg ccagtttgca cagcgtttgc agcaacagcg tggatttccc
    31141 cgcaccaggg ttaccgccaa tcagaatggc acttcctggc accacgccgc ctcctagtac
    31201 gcggtcgaac tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga
    31261 gagtttctgg acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg
    31321 cgccaccgtt ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc
    31381 actgcactgc ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg
    31441 ttttggagct tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatgacgggc
    31501 aggcaattac ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc
    31561 gtgacggatg gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc
    31621 cagccctgct gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg
    31681 cgccagcggg atttcatact cctgcgcgag gcgagtcaga gttttcgctt tgtactgcgc
    31741 gtctacgatg tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt
    31801 ggctaccacg gctgtcaggc gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc
    31861 gccggaggca atcgccactt tccagcccag cgtttccagc ttgagcacca gctgcgttaa
    31921 gcctggcatc agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt
    31981 cagcgtcgcc acgcggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg
    32041 ttcggttacc tccgccacca tctcgcccgt tccggccagc ttggcaattt catcaataca
    32101 ttcaatctgg atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg
    32161 cgggattttc cccagcgggg cgacatccag ctgcgcttcg tgggccaggc gtgtagcccg
    32221 tgcggtgagt gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac
    32281 aatcaccatc gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc
    32341 acgaccatac agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc
    32401 accacttaat gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca
    32461 ggtaatgtta ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga
    32521 ggctaccttg tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag
    32581 atggctcgca caaaactgaa attccggcta catcgggcag tgattgtcct gttctgtctt
    32641 gccttgttag tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag
    32701 cgtaatccac agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt
    32761 gcaccgctga tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag
    32821 ttaacagatg aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc
    32881 gcacgttctg gcgaaagcgt cgaagtgcgc gatcggctgg cgctcgacgg taaaaaagca
    32941 ggcggctatt ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc
    33001 tatctgcgcc tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac
    33061 accactaaca ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc
    33121 cgcacgctgc tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc
    33181 agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga
    33241 aaggaaatcg ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt
    33301 aacctggcgg tggaagagtg tatttttcgc caaatgcccg ccacgcagcg cgttctgttt
    33361 ctctggcgca atgccgacac ggtagtaatt ggtcgcgcgc agaacccgtg gaaagagtgt
    33421 aatacccggc ggatggaaga agataacgtc cgcctggcgc ggcgcagtgg cggtggcggc
    33481 gcggtgttcc acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac
    33541 gataaaacta tctccacgtc gattgtgctc aatgcgctga acgcgctcgg cgtcagcgcc
    33601 gaagcgtccg gacgtaacga tctggtggtg aaaaccgtcg aaggcgaccg caaagtctca
    33661 ggctcggcct atcgcgaaac caaagatcgc ggcttccacc acggcacctt gctactcaat
    33721 gccgacctca gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa
    33781 ggcattactt cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggctcacc
    33841 atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc ccattatggc gagcacgtgg
    33901 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg
    33961 cccgtcagag tagctgagag tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    34021 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    34081 cccgcgccca ggtatttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc
    34141 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    34201 ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg
    34261 taaggtaact cgctgattaa aatatccaag gaaggaaata tgatcgatct ggaaaaccag
    34321 gaacgggaga ttatcaattt aatgttcagc cagcgtattt cctggctagc cgccgtgcgt
    34381 atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct
    34441 ctaaaacaga tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatggcagaa
    34501 atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa
    34561 tgaatagatt cgtgaataga tacttgaatg ttttttattt tctatccata tgaaaaataa
    34621 tagtaaatct ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc
    34681 ccactccggc ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca
    34741 gcagaacgga ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg
    34801 cagtggtctg ctcgtgagtg cggatgtggt cagacacggt gcagatggtc agggctttcg
    34861 cgccaaactc tgcagccacg ccgtagatac cagccgcttc catttccacg ccgaggatgc
    34921 cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga
    34981 acaggttacc cacgcgagca tcaacgccca gtgctttagc tgcatctact gcgttacgca
    35041 ccatgtcaaa gtcagcgata gcggcaaagt catggtcttt aaaacggatg cggttaactt
    35101 tggaatcggt gcaggccccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa
    35161 ctgcgccaca ggaaccaacg cggataattt tcttcacgcc gaaatcggtg atcagttctt
    35221 tggtgtagat ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc
    35281 ctttgtaagt accagtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt
    35341 caaggaaagt ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt
    35401 cagcgaaatc gcccatttct gcattaatgt gtggggtagc cattgtttta tcctttttgt
    35461 gacataacaa aggcggagtc aacgctccgc ccaaatccat cagaacatgg ctttgccata
    35521 ttccatatca gaagtaccaa aatattttgc cagagtctgg ccgatatccg cgaaggtttc
    35581 acggtgaccc agtgagcccg gttttacttt cgggccatat accagtaccg gaatgtgttc
    35641 acgcgtgtgg tcagtaccgg tccaggtcgg atcgcaaccg tggtcagcgg tgaggatcag
    35701 gatgtcgtca tcgcgcagca gagacatcag ctccggcaga cggcggtcga acagttccag
    35761 acccgcagca taaccggcga cgtcgcggcg gtggccccag gaagagtcga agtcaacgaa
    35821 gttggtgaag acgatggtgt tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa
    35881 cagcgcgtcc aagccagtcg ctttcacttt tttggtgata ccgcagttgg cgtagatgtc
    35941 tgcaatttta ccgacagaaa ccacctggcc gtgtttttca tcaaccagtt tctgcagcac
    36001 ggtcggtgct ggcggctcaa cagccaagtc gtgacggtta ccggtacgct ggaagttacc
    36061 agctttgtca ccgataaacg gacgagcgat aacacgaccg atattgtagc cgccgttggt
    36121 cagctcttca cgggcgattt cgcacagttc gtagagttta tccagaccga aagtttcttc
    36181 atggcaggca atctggaaca cggagtcagc ggaggtatag aaaatcggct tgccggtttt
    36241 catgtgctct tcgcccagtt gatccagaat gaccgtaccg gaagagtggc agttaccgag
    36301 gtaacccggc agattagcgc gttcgaccag tttatccagc agctcttgcg ggaagctgtt
    36361 ttcgtgatcg gagaaatatc cccactcaaa cagaaccggg acaccggcga tttcccagtg
    36421 accagacggg gtatctttac cggatgacat ttcgtgcgcc catgcgtacg cgccgataac
    36481 ttcagcgttg ccgtccattc ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc
    36541 cagccccaga cgggtcagat ttggcagatt gagcggccct ttacgaccat tatcagcttc
    36601 gcctttggca caagcttctg cgatatgacc cagggtgtca gccccgacgt caccaaagcg
    36661 ttctgcatct tctgtagcgc cgatgccgaa tgagtccagc accataataa atgcacgttt
    36721 catatgttct ccgtactcag tgcttcaaat ttaaatgatc agatcagtat accgttattc
    36781 gctgatacgg cgatagacag ttggtgtgct ttccggtgcg ttatcggcaa gtttaattgc
    36841 cgctttcacc gctttcgccg cttcctgcca gctgttttcg tctttcgcga ggataaccgc
    36901 cagcggacgc tgaccgtcta cctggtcgcc cagacgcgcc atatcagtaa agccgacgct
    36961 gtaatcgatg gtgtcagatg cctgacgacg accgccgccc attgcaacta ctgccatccc
    37021 cagcgcgcgg gtatccattt cactgacaaa accttcggta tcagcataga ctgctttcgt
    37081 cagcatcgct gtcggcagat acttcgcgta gttctcaacg aagtcggtcg ggcctttttg
    37141 tgccgctacc atacgaccaa agacttctgc cgctttaccg ttgtccagca ccgcctgcaa
    37201 tttcgcgcgc gcttcggcgt catctttcgc cagtttgccg gagatcaaca tctccacgca
    37261 cagcgccatc gtgacatcaa acagacgcgg gttacgatac tcacccgtca ggaactgcac
    37321 cgcttcacga acttcaaccg cgttacctgc actggaggcc agtacctgat tcatgtcggt
    37381 gagcagcgcg gtggtgcgca cgccagcgcc gttagccacg ccaacaatcg cttcggcaag
    37441 ggcttcagag agttcgtagg tcggcataaa cgcgccgcta cccactttca cgtccatcac
    37501 cagcgcgtcc agaccttccg ccagtttctt cgccaggatc gaggcagtga tcagcgggat
    37561 ggagtccacg gttgcggtaa tatcacgggt cgcgtagaaa cgtttatcag ccggagccag
    37621 tgaactggtc tgaccgataa tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt
    37681 gtcatccggg aaaatgtcga agccagggat ggattccagt ttgtcgagcg taccgccagt
    37741 atgaccgagg ccgcgaccgg agatcatcgg aatatagccg ccgcaggctg cgaccatcgg
    37801 ccccaacatc agcgaagtga catcgccgac gccgccggtc gagtgtttat caacaatcgg
    37861 gccattcaga tgcaggcttt tccagtcgag aacggttcct gaatctcgca tcgccatggt
    37921 cagcgagaca cgctcaggca ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc
    37981 aatctgccct tcggaaatag tgttgtcgcg aataccgttg ataaagaaac gaatttcttc
    38041 atcgctcagc gcatgaccat cacgtttttt acgaataatt tcttgtgcga gaaacaaggt
    38101 aacctcccga ggaaaagagt atgaggcggc ttttgccgga tgcgtctacg ccttatccgg
    38161 cctacgggta gatacgtctc gtaggcctga taagcgcagc gcatcaggcg taaagcatct
    38221 tacttagtag ctgctggcgc tcttaccgtc gccgtggccc agcgctttca gcaggcttgc
    38281 cagcaggctg gaagcaccaa agcggtagtg acgcgcatca gcccagtcag caccgaacag
    38341 ttcatctgcg atggcgagat atttctgcgc atcttccgca gtacgcacgc cgcccgccgg
    38401 tttgaaacca acggtttttt ctacgcccat atcacggatc acttccatca tgatgcgagc
    38461 gctttccggc gtcgcgttca cagccacttt accggtagag gttttgatga agtccgcacc
    38521 cgctttgatg gagatttcag acgctttacg gatcagcgct tcgtctttca gttcgccggt
    38581 ttcgatgatc actttcagca gtacattcgc tgccgcgcaa gcctctttac aggctttcac
    38641 caggtcaaaa ccaacctgct cgtcacccgc catcagcgcg cggtacggga acaccacgtc
    38701 aacttcatca gcaccgtagg cgattgccgc acgggtttct gccagcgcga tttcgatgtc
    38761 gtcgttaccg tgtgggaagt tggttaccgt agcgatacgg atttccgggg tgccctgctc
    38821 tttcagagtt ttgcgagcaa tcgggataaa acgaggatag atacagatag cggcggtatt
    38881 gccgaccgga gttttggcct gatgacacag agcaattact ttctcgtcgg tgtcgtcgtc
    38941 attcagggtg gtcaggtcca tcaatttcag tgcacgcagg ctgcttgctt tcagatcagt
    39001 catttcattc tcctggcttg tcgccaataa aattcacctt gcgagtttgt tagtattcta
    39061 acatctactc cgcaacacac ttcgatacac atcacaatta aggaaatcta cttacaagtt
    39121 tgcatcactg taatgcgatc tggttcaaat aattcacttt caaatgaatg cgtcagtggt
    39181 ggcaaacgca tcaggatcaa agtgaacatc acgaaacttc ttacaatggc gcatcacctt
    39241 cggcataaaa ggaaaagaag atgcccactt ctcatgaaaa tgcactgcaa caacgttgcc
    39301 agcaaattgt caccagccca gtgcttagcc cggagcagaa gcgccatttt ctggcactgg
    39361 aagcagaaaa caatctgcct tacccacagc ttcctgccga agcccgccgc gcgctggatg
    39421 aaggtgtaat ctgcgatatg tttgaaggtc atgcgccgta caaaccgcgc tatgtcttac
    39481 ccgattacgc tcgttttctg gcgaacggtt ccgaatggct ggagctggaa ggcgcgaaag
    39541 atcttgatga cgcactctct ctgctgacca ttctttacca ccacgtaccg tcggtcacat
    39601 cgatgccggt ctacctgggg caactggatg cgttgttgca accgtatgtt agaattctaa
    39661 cacaaaacga gatcgatgtt cgaataaaac gtttctggcg ttacctcgac agaaccctgc
    39721 cagatgcctt tatgcacgcc aatatcggcc cgtctgattc gcccattacc cgcgcgatct
    39781 tacgtgcaga cgcgcaggtt tcaccgaacc tgacctttat ctacgatcct gaaatcaccc
    39841 ctgatgacct gctgctggaa gtggcgaaga acatctgtga atgtagcaaa ccgcacatcg
    39901 ccaacggtcc ggtgcatgat aaaattttca caaaaggggg ctacgggatt gtgagctgtt
    39961 acaactcact gccgctggcg ggtggtggca gcacgctggt acgccttaac ctgaaagcca
    40021 ttgccgagcg cagcgaatcg ctggatgact tctttacgcg cactctaccg cactactgcc
    40081 agcagcagat cgccatcatc gatgcgcggt gtgaattcct ctatcaacaa tcacacttct
    40141 ttgagaatag cttcctggtg aaagaagggc tgattaaccc tgaacgtttt gtgccaatgt
    40201 ttggcatgta tgggctggcg gaagcggtta acttgctgtg tgagaaagaa gggattgccg
    40261 cgcgctacgg taaagaagcc gccgcaaatg aagtgggtta tcgcatcagc gcgcaactgg
    40321 cggagtttgt cgccaatacc cccgtgaaat atggctggca aaaacgcgcc atgttacacg
    40381 cacagtcggg gatcagttcc gatatcggca ccacgccggg cgcgcgtttg ccgtatggcg
    40441 atgagccaga tccgatcacc catctgcaaa ctgtcgcccc gcatcatgct tattattatt
    40501 ccggcatcag cgacattctg acgctcgacg aaaccatcaa acgtaacccg caggcactgg
    40561 tacagctttg cctcggtgcc tttaaagccg gaatgcgtga atttaccgcc aatgtcagcg
    40621 gtaacgatct ggttcgcgtt accggttata tggtgcgttt gtcggattta gaaaaatatc
    40681 gcgccgaagg ttcacgcacc aacaccacct ggctgggcga agaagccgca cgcaacactc
    40741 gtattctgga acgccagccg cgcgtgataa gccatgaaca gcagatgcgc tttagtcagt
    40801 aagattatcc ccttctcctg cgttgacggg ccaggcagtc gtctggctct gtttttgcag
    40861 ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga cgatgggacg ttgcaatgac
    40921 tgtggggagt gcgtgccaca gtgtccgcat caggcgttgc agattgttga cggcaaagtg
    40981 gtgtggaacg ctgtggtctg cgagcaatgt gatacctgcc tgaagatgtg tccgcaacat
    41041 gccacaccaa tggcgcaatc catgagcgtg gacgaagtgc ttagccatgt gcgcaaagcg
    41101 gtgctgttta ttgaagggat aacggtaagc ggcggcgaag ccacgaccca actgccgttt
    41161 gtagtggcgc tgtttactgc tatcaaaaac gatccgcaac tgcgccatct cacctgtctg
    41221 gtggacagta acggtatgtt gagcgaaacc ggctgggaaa aattactccc ggtgtgtgac
    41281 ggcgcaatgc tcgatctcaa agcatggggt agcgaatgtc atcaacaatt aaccggacgc
    41341 gataatcaac agattaagcg cagcatctat ttgctggcag agcgcggcaa gctggcggaa
    41401 ctgcgtttgc tgatgatccc aggccaggtg gattatttgc aacacatcga agaactggcg
    41461 gcgtttatca agggacttgg tgatgttccg gttcgcctga acgcgtttca tgcccacggc
    41521 gtgtatggcg aggcgcaaag ctgggcgagc gccacgccgg aagacgttga gccgttggct
    41581 gatgcattaa aggtgcgcgg ggtgagccgg ttgatatttc cggcgctcta tttgtgacat
    41641 gccggatacg gcctacggtt cggcacagac ttgtaggcct gataagacgc gtcaagcgtc
    41701 gcatcaggca ttgtgcgcca actgccggat gcggcgtgaa cgccttatcc ggcctacggt
    41761 tcggcacaaa cctgtaggca tgataagacg cgttaagcgt cgcatcaggc attatgcgcc
    41821 gactgccgga tgctgcctac gaagggcaaa ctacccacga acgttaaaca ccgcatacgt
    41881 gttattaagc aacacttcgg caatctcatc cgcgggttcc gggcgtaact cgcaaagcac
    41941 ggcaaacaca cgggcagcct gttccgggcg gttaggctgc ccctgaaaac cgttgagcgg
    42001 catatccggc gcatcggttt ccagcaacaa cgatgccagc ggtaattttg cgatgacatc
    42061 gcgggtttta ctggcgcgtg gataggtgat agtaccgcct acgccgattt tgtagcccag
    42121 ctgtacaaac cgttcagcct gttgctggct gccagaaaat ccgtggacaa caccagcacg
    42181 cggcaaatcg tggcgtttaa gatgcatcgc cagtttgtcg tgcgtgcgcc gtgaatgcag
    42241 gatcaccggc agatcgtagc gtttcgccag tttcagttgt tcgtcgagta accactgctg
    42301 cctctcaaat tgcggatcgt cgccgaagag atccagaccg atctccccca ccgccaccac
    42361 cttcgccgga cgcctttcca gcgcctgctg tagctgctca agagacacat cgctatgttt
    42421 ttccaacata ccaggatgca agcccaatac ggcatacagc ggttgataat tttccgctaa
    42481 cgcctgcaca cgggcaaaat tttccgcctc tgtcgccgga acaatgatct tgcctacgcc
    42541 cgcttgtgcc gcgcgttgca ggctggcctc ttcatcgcca ataaaaggcg ggaaatcaaa
    42601 atggcagtga gtgtcgataa aacggcaaat caagccagat cctcattgtt aaatgtcggg
    42661 tcgttcgcct gcggagcgtc gctcacttcg gcaaccagcg tatcgttggc gactggcgca
    42721 ggcggaatga caatcgattc cggcgtcacc actggcacca gatggcgggt aagcggcgct
    42781 ttttcagtta atagcttgcc aaccgtggcg aggaaataac gaccgcataa acgcccaagt
    42841 ttatagtctt cacgcagcgc cggaatccga ctgccaagcg cgatactatg taatggcttc
    42901 ggcggataaa tttcggatat ccgcagcttg cccggtggtt tctcaataaa ttgctgaatg
    42961 tcacgatagc tggtttcatg atgctgcacc agattgacca gcggctgcag gctactgtca
    43021 cccagccagc gttccatgcg tttgaaccac tgcggcgtgt agtacatttg tgacggcaca
    43081 gtgcggatga cgaccaacgt tttagcgccc tgccttgccg cctctttaac cggaatcgca
    43141 tcactgatcc cgccatccag gtagttaatg ccttccagcg acactccgct acgataaaag
    43201 ccaggtatcg cactggaggc gcgaatcaca tccagccagt tttgtttggt tggtaaaaag
    43261 taattcggcg cgtagtcatc ctgacgacag gcgcacatat aaaacgattt gccgctgtca
    43321 aacaaccgcg cggcggtatc catttgcaac ggcatctggc ttgcggtggc ctccaccagc
    43381 cagtcgagat cgataagatt tcctccacgg acaaagcgca atggatcgaa aaattcgcgt
    43441 tttgtggtat agcgcatgat gactttgcgc gcgtaaccgg gctgattgca gataaacgcc
    43501 gagaggttct gcgccccggc agatgtgccg agataaagat cgaaaggatt aaactgcgcg
    43561 cgcataaact catccagcac gccagccgtg aaaattccac gctgtccgcc gccttcgcac
    43621 accagagcta ttcgtccagg ctggaacggc cttagcgaca acggcgcaat attaccaagc
    43681 gttacaggta ttcgctgccc cacttctgcc ttcctgtttt tgttgtgaaa gaaagacaaa
    43741 gtaacgcaat ttattattcg ctaaaaccgc aaaagccagt ccgctggact ggctttggat
    43801 aaaattttct taatggtctg aaatctaggg tcgttttcgg cccatgaaca aactcactag
    43861 gaacagaata atcccgacga caaagacaat tttagctgcg cctgcagcgg taccggccag
    43921 accaccaaac ccaagtgcgg cggcgattaa cgcgataacc agaaatatga tgccccaacg
    43981 aaacataagc gtctccttta ccatagttaa tgtcaccgct tgatatgagc gtgaaaatca
    44041 ctcaatgcga tatttttagt gtggtgcaca tcgcgcctcc cgacaaagtt cgggaggacg
    44101 aattacttag ttttcagatc atttttaacg cttttcacac catctaccgc tttggcgata
    44161 ctttcagcac ggtcactttg tgcctgagaa tctacggtac cggagagctg aaccacgcca
    44221 tcggtggttt caactttcac tttacgtgaa gggacgatat cgtccgccag cagtttggct
    44281 ttgatttcac tggtggtggc ggtgtcaccc gcgtagccct tcaccgagcc ttctttagcg
    44341 tcgcgaacgt gcagtttgtc gctgacagag gtcacgcctt caacgccttt cgccactttc
    44401 actgcctctt cggcctgggc ctgactttca acgaaaccgc tcagggtcac gactttttga
    44461 tcggttttga cagagatatc ggtgctcttg atgttgtcat gatccaccag cgccgccttc
    44521 actttcgcgg tgatggcgct gtcatccatg aaattaccga ctttattcat agagctatcg
    44581 actttttgcc ctgcgctttc attggtagtc tgcgcgttgt tttccgcgta ggcagagccg
    44641 gtcgcgacgg cagaggtcaa cattacagcc agcagagttt tcgaaatctt cagtcttgtc
    44701 atagtcatcg atttattcct gtatgtttgc tcgtaatttg agcctagcaa cacgaggttg
    44761 cattgctgaa tagggagaga cttcaccctc tacagaagtc aatggtcgcc atcacaaaag
    44821 cgatgggtga tgaataacga ccattacagc ctctgaatca gttattaata tcggtagaat
    44881 gacaatcgac ggctttagat actaatatct acgcattgaa cggtatttaa cgccatcaga
    44941 aatgtcatca ctttgttaaa tatagatcac aattttgaaa ccgctcggga tatcagcgaa
    45001 aacataagca aaagtgaatg ttttaagaac attccgtaag cggctaataa ggaagggaaa
    45061 ttgacagggc gcagcggcta ccgcgccctg gaggcaagaa ttaatgctcg cgggtctggt
    45121 ggaactgaac gtccggataa cgttcctgtg ccaggcgcag gttgaccatg ctggtagcga
    45181 tgtaagcgag gttatcgccg ccatcaagcg ccagttggct ttcgttctta cgcttgaact
    45241 cttcgaattt cttcgcgtct gcacattcta cccagcgggc agtggcaacg ttgactgact
    45301 catacactgc ttcaacgttg tattcgctct tcaggcgcgc taccaccaca tcaaactgca
    45361 gcacaccaac cgcaccaacg atcagatcgt tgttggagat tggacggaac acctgcaccg
    45421 cgccctcttc ggaaagctgt accagccctt tgagcagctg tttttgcttc agcggatctt
    45481 tcaggcggat acgacggaac agttctggtg cgaagttcgg aataccggtg aacttcatca
    45541 tctcaccctg ggtaaaggtg tcgccgatct gaatggtgcc gtggttgtgc aggccgagga
    45601 tatcgcccgg atacgcttct tcaacgtgcg aacggtcacc cgccataaag gtcagcgcgt
    45661 cggagatcac cacatctttc gcagtgcgca cctggcgcag tttcatgcct ttttcatatt
    45721 taccggacac cacacgcata aacgccacgc ggtcgcggtg tttcggatcc atgttggcct
    45781 gaattttaaa tacgaagccg gtaaatttat cttcgctcgc ttctacggta cgggtatcgg
    45841 tctgacgcgg catcggcgca ggcgcccact ccaccaggcc attcagcata tgatcaacgc
    45901 cgaagttacc cagcgcagta ccgaagaata ccggagtgat ttcgcccgca aggaacagct
    45961 ctttgtcgaa ctcgttagac gcgcctttaa ccagttccag ttcgtcacgc agctgctgtg
    46021 ccagatcttc accaaccgca gcatcgagat ccgggttatt cagcccttta acaatgcgga
    46081 cttcctgaat ggtgtgccct ttaccgctct gatagagata ggtttcatct ttataaaggt
    46141 ggtaaacgcc tttaaacagc ttgccgcagc caattggcca agtgatcggt gcgcagccaa
    46201 ttttcagctc gttctcaact tcatcgagca gttccatcgg gtcgcggata tcacggtcaa
    46261 gtttgttcat aaaggtgagg atcggcgtgt cgcgcagacg ggtaacttcc atcagcttac
    46321 gggtacgatc ttcaacacct tttgcggcgt cgataaccat caggcagcag tccaccgccg
    46381 tcagggtacg ataggtatct tccgagaagt cttcgtgccc cggggtgtcg agcaggttaa
    46441 ccaggcaatc gtgatacgga aactgcatca cagacgtagt aatggagatc ccacgctgct
    46501 tttccatctc catccagtcc gacttagcgt gctggttgga accacggcct tttactgtac
    46561 cggcggtctg aatggcctgt ccgaacagca gcaccttctc ggtgatggta gttttaccgg
    46621 cgtccgggtg agaaataatg gcaaaagtgc ggcgcttcgc cacctctttc aaataaggag
    46681 acaacgtcat aattctttct tcttgagtaa gcggcatcgc gccgcgcttg ttgaaaacga
    46741 aaaattgcgg ctattttacc catcaacggg ggggaggcaa tcagtgttta cacaggagct
    46801 gctccagttc gtgcaacgaa gaaacggtcc aggtgggcgc gatgccttct ggttgctcgc
    46861 gattgtgcgc attcagccag caggtcgcaa gcccggcgtt gatgccaccg agaatatcgg
    46921 actcggcagt gtcgccaacc atcagcacgc gtgaacggtc aggattgccc gcctgttcca
    46981 gcgcataatc gaaaattttc ttattcggtt tggcaacgcc aacttcttcg gaaatcacca
    47041 gcaaatcgaa gtaatcacgc aggcccgtcc gttccagacg cacttgctgc aaggcgctaa
    47101 aaccgttggt gatgatgccg attttagcgt tgctacgaat ggcgttaagc agagaaaccg
    47161 cgcccggcaa cggcgtacag atttccgcca tcgcattaat aaaggcatcg ttaagcaagc
    47221 ctgcttcaac tttcagccgt tcggcccagc tctcgaaacg cccgtgctga agctgtaatg
    47281 aagtgatcgc gccgttttga taatccaccc acagtggctt gttaacggcc tgatagtcct
    47341 gaaaatcttc agcggtaaag gtgacgctgt aatcaagaaa catccgctgc aggccggtga
    47401 atgagtcaaa ggtaaacagc gtttcatcgg catcaaagaa aatccagtcc cacttcatta
    47461 ttccaccttg tcttacatac tgattggcaa cgccatgatg atggcgtctt cgcgaccgtc
    47521 cgtggtgggg tagtaattgc ggcgaatcgt cgcctcgtta aagcctaaac tttcgtacag
    47581 ggcaatggcg gcagcatttg aggcacggac ttccagccat agtgtcgcca cgccgcgttt
    47641 ttccagttcg tcgatcagat gttccagcag cgccctaccc agcccctgac gctgatagtc
    47701 gggatcgacc gcaatattga acaatgtagc ttcatccagc accacttgcg taatcgcaaa
    47761 cgccgccatt ttgccgtttt gcgttaactg aaagttgaga taacgctcgc cctggttgct
    47821 ggcaaaggtt ttttcactcc acggaaaggc gtgggcgcgt tgttcaatgt ggtaagccgc
    47881 tggtaaatca gtcgtttcga gggaagaaat cgtgttcata tgtgcaaatt tgttgccata
    47941 acgcggcgcg tgccgttggg tttgcccgta attcggtgag cgccggtgat gccacctgag
    48001 cgccttccaa tgatagcggt tcgtcagtac ccaaccgcca gctgttgcag cgactgcctt
    48061 gcggcagcat cgcgattttt tctggcgtca gttgcagcac ttgatcgggg ctgacggtta
    48121 atgcgcgcag aacatcgctc actaaaggat cagtcagggc gggaagatcg tttgccacca
    48181 tcaccagacg gacgtgtgcc gggctcgcaa tggcaatctc cccctgcaac gcgccagggc
    48241 gacgcagcga ccactgggta atgcccagtt gctgtaactg ccagtctcgt cgggatgtca
    48301 tagcgaatcg ctcctgttgt caggggcgca aatatagcaa attcgtcgat accgcgccaa
    48361 catatggcta taatcgccgc cagtatcaat tgaggagcat tccatgtctg catttacccc
    48421 ggcaagtgaa gtcttgctgc gtcacagtga tgatttcgaa caaagccgta ttctgtttgc
    48481 cggagactta caggatgacc tgcccgcgcg tttagatacc gcggccagcc gtgctcatac
    48541 ccagcaattc caccattggc aggtgttaag ctgccagatg ggggataacg cccgttttag
    48601 tctggtcgcc acggcgaatg atgtcgcaga ttgcgatacg ctgatttact actggccgaa
    48661 gaacaaaccg gaagcccagt tccagttgat gaatttactt tctctgctgc cagtggggac
    48721 agatattttt gtcgttggcg agaaccgcag cggcgtgcgc agcgctgagc agatgctggc
    48781 agattatgcg ccgttgaata aagtcgacag cgctcgtcgc tgtggcctct attttggtcg
    48841 tctggaaaaa cagccagtat ttgatgcgga taaattctgg ggcgaataca gcgtcgatgg
    48901 cctgacggtc aaaacgctgc ctggcgtgtt tagccgcgac ggtctggatg tcggtagcca
    48961 gttgctgctc tcgacgttaa ctccgcacac gaaaggtaaa gtgctggatg tcggctgtgg
    49021 cgcgggtgta ctttcagttg cctttgcgcg ccattcgccg aaaattcgtc tcaccttgtg
    49081 cgatgtctct gccccggcgg tagaagccag ccgcgcaaca cttgcagcca acggtgttga
    49141 aggtgaagtc tttgccagca acgtcttttc cgaggtgaaa ggttgttttg atatgatcat
    49201 ctccaacccg ccgttccacg atgggatgca aaccagcctg gatgcggcgc aaacgctgat
    49261 tcgtggcgcg gtgcgtcatc ttaatagcgg cggcgagctg cgaattgtag cgaacgcctt
    49321 cctgccttac ccggacgtgc tggatgagac atttggcttc catgaagtta tcgcgcaaac
    49381 cgggcgcttc aaggtatatc gcgccattat gacccgccag gcgaagaaag gttgagtatc
    49441 tgatatctgt cgccgcatac cgtgtcggat gcggcgtgaa cgccttatcc gacctacaaa
    49501 acctgcacgt tagcccttcg taggccagat aagacgcgcc agcgtcgcat ctggcatctg
    49561 cccccgcatg cctaccaaac ctgcacgcta gtttcctgat ggacattttt ccagcaatta
    49621 cacatcaggc gataattaac tattgacgaa aagctgaaaa ccactagaat gcgcctccgt
    49681 ggtagcaatt ctttttaaga attgatggta tgcgaaggtg gcggaat
//
LOCUS       DAZPEU010000008        49516 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPEU010000008 DAZPEU010000000
VERSION     DAZPEU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.008"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHU2L_000386"
     CDS             <1..1224
                     /locus_tag="ACHU2L_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1355390.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHU2L_000387"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHU2L_000387"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1355391.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHU2L_000388"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHU2L_000388"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR1355392.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHU2L_000389"
     CDS             2714..3427
                     /locus_tag="ACHU2L_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR1355393.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHU2L_000390"
     CDS             complement(3399..4076)
                     /locus_tag="ACHU2L_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1355394.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHU2L_000391"
     CDS             complement(4070..4747)
                     /locus_tag="ACHU2L_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1355395.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHU2L_000392"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHU2L_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHU2L_000393"
     CDS             complement(5443..6021)
                     /locus_tag="ACHU2L_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR1355396.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHU2L_000394"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHU2L_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHU2L_000395"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHU2L_000395"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR1355397.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHU2L_000396"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHU2L_000396"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR1355398.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHU2L_000397"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHU2L_000397"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR1355399.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHU2L_000398"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHU2L_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR1355400.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHU2L_000399"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHU2L_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR1355401.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHU2L_000400"
     CDS             12291..13031
                     /locus_tag="ACHU2L_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR1355402.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHU2L_000401"
     CDS             13198..14148
                     /locus_tag="ACHU2L_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1355403.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHU2L_000402"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHU2L_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHU2L_000403"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHU2L_000403"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR1355404.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHU2L_000404"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHU2L_000404"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR1355405.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHU2L_000405"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHU2L_000405"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR1355406.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHU2L_000406"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHU2L_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR1355407.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHU2L_000407"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHU2L_000407"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR1355408.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHU2L_000408"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHU2L_000408"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1355409.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHU2L_000409"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHU2L_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR1355410.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHU2L_000410"
     CDS             23768..23827
                     /locus_tag="ACHU2L_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1355411.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHU2L_000411"
     CDS             complement(23928..24530)
                     /locus_tag="ACHU2L_000411"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR1355412.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHU2L_000412"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHU2L_000412"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHU2L_000413"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHU2L_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR1355413.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHU2L_000414"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHU2L_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR1355414.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHU2L_000415"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHU2L_000415"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR1355415.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHU2L_000416"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHU2L_000416"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR1355416.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHU2L_000417"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHU2L_000417"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR1355417.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="ACHU2L_000418"
     CDS             complement(29676..29759)
                     /locus_tag="ACHU2L_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355418.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHU2L_000419"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHU2L_000419"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR1355419.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHU2L_000420"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHU2L_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR1355420.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHU2L_000421"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHU2L_000421"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR1355421.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="ACHU2L_000422"
     CDS             34816..35559
                     /locus_tag="ACHU2L_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1355422.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACHU2L_000423"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACHU2L_000423"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR1355423.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /gene="yqfB"
                     /locus_tag="ACHU2L_000424"
                     /pseudo
     CDS             37094..37404
                     /gene="yqfB"
                     /locus_tag="ACHU2L_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            37568..38227
                     /locus_tag="ACHU2L_000425"
     CDS             37568..38227
                     /locus_tag="ACHU2L_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR1355424.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="ACHU2L_000426"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="ACHU2L_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR1355425.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="ACHU2L_000427"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="ACHU2L_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR1355426.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="ACHU2L_000428"
     CDS             39893..40300
                     /locus_tag="ACHU2L_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR1355427.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="ACHU2L_000429"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="ACHU2L_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR1355428.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="ACHU2L_000430"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="ACHU2L_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR1355429.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="ACHU2L_000431"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="ACHU2L_000431"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR1355430.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="ACHU2L_000432"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="ACHU2L_000432"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR1355431.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="ACHU2L_000433"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="ACHU2L_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR1355432.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="ACHU2L_000434"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="ACHU2L_000434"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1355433.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="ACHU2L_000435"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="ACHU2L_000435"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR1355434.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="ACHU2L_000436"
     CDS             47705..47776
                     /locus_tag="ACHU2L_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355435.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /gene="yqfG"
                     /locus_tag="ACHU2L_000437"
     CDS             complement(47773..47898)
                     /gene="yqfG"
                     /locus_tag="ACHU2L_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR1355436.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="ACHU2L_000438"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="ACHU2L_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR1355437.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11954 a        12397 c        13344 g        11821 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg actgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 tcgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgcagtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaa
//
LOCUS       DAZPEU010000009        48494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPEU010000009 DAZPEU010000000
VERSION     DAZPEU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.009"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            361..1578
                     /gene="alaA"
                     /locus_tag="ACHU2L_000439"
     CDS             361..1578
                     /gene="alaA"
                     /locus_tag="ACHU2L_000439"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR1355438.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1662..2261
                     /gene="yfbR"
                     /locus_tag="ACHU2L_000440"
     CDS             1662..2261
                     /gene="yfbR"
                     /locus_tag="ACHU2L_000440"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR1355439.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2320..4152)
                     /locus_tag="ACHU2L_000441"
     CDS             complement(2320..4152)
                     /locus_tag="ACHU2L_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1355440.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4239..4889)
                     /gene="hxpA"
                     /locus_tag="ACHU2L_000442"
     CDS             complement(4239..4889)
                     /gene="hxpA"
                     /locus_tag="ACHU2L_000442"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR1355441.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4900..5394)
                     /locus_tag="ACHU2L_000443"
     CDS             complement(4900..5394)
                     /locus_tag="ACHU2L_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR1355442.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5477..5932)
                     /gene="yfbV"
                     /locus_tag="ACHU2L_000444"
     CDS             complement(5477..5932)
                     /gene="yfbV"
                     /locus_tag="ACHU2L_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR1355443.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6270..7472
                     /gene="ackA"
                     /locus_tag="ACHU2L_000445"
     CDS             6270..7472
                     /gene="ackA"
                     /locus_tag="ACHU2L_000445"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR1355444.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7547..9691
                     /gene="pta"
                     /locus_tag="ACHU2L_000446"
     CDS             7547..9691
                     /gene="pta"
                     /locus_tag="ACHU2L_000446"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1355445.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9881..11401
                     /gene="yfcC"
                     /locus_tag="ACHU2L_000447"
     CDS             9881..11401
                     /gene="yfcC"
                     /locus_tag="ACHU2L_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR1355446.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11434..11976)
                     /gene="yfcD"
                     /locus_tag="ACHU2L_000448"
     CDS             complement(11434..11976)
                     /gene="yfcD"
                     /locus_tag="ACHU2L_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR1355447.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12034..12585)
                     /gene="yfcE"
                     /locus_tag="ACHU2L_000449"
     CDS             complement(12034..12585)
                     /gene="yfcE"
                     /locus_tag="ACHU2L_000449"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR1355448.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12641..13285)
                     /gene="yfcF"
                     /locus_tag="ACHU2L_000450"
     CDS             complement(12641..13285)
                     /gene="yfcF"
                     /locus_tag="ACHU2L_000450"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR1355449.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13421..14068
                     /gene="yfcG"
                     /locus_tag="ACHU2L_000451"
     CDS             13421..14068
                     /gene="yfcG"
                     /locus_tag="ACHU2L_000451"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR1355450.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14125..14487
                     /gene="folX"
                     /locus_tag="ACHU2L_000452"
     CDS             14125..14487
                     /gene="folX"
                     /locus_tag="ACHU2L_000452"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR1355451.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14508..15401
                     /locus_tag="ACHU2L_000453"
     CDS             14508..15401
                     /locus_tag="ACHU2L_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR1355452.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15449..16339)
                     /gene="rpnB"
                     /locus_tag="ACHU2L_000454"
     CDS             complement(15449..16339)
                     /gene="rpnB"
                     /locus_tag="ACHU2L_000454"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1355453.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16536..17309)
                     /gene="hisP"
                     /locus_tag="ACHU2L_000455"
     CDS             complement(16536..17309)
                     /gene="hisP"
                     /locus_tag="ACHU2L_000455"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR1355454.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17317..18033)
                     /locus_tag="ACHU2L_000456"
     CDS             complement(17317..18033)
                     /locus_tag="ACHU2L_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1355455.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18030..18716)
                     /gene="hisQ"
                     /locus_tag="ACHU2L_000457"
     CDS             complement(18030..18716)
                     /gene="hisQ"
                     /locus_tag="ACHU2L_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR1355456.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18806..19588)
                     /gene="hisJ"
                     /locus_tag="ACHU2L_000458"
     CDS             complement(18806..19588)
                     /gene="hisJ"
                     /locus_tag="ACHU2L_000458"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR1355457.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19809..20591)
                     /gene="argT"
                     /locus_tag="ACHU2L_000459"
     CDS             complement(19809..20591)
                     /gene="argT"
                     /locus_tag="ACHU2L_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR1355458.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20857..21426)
                     /gene="ubiX"
                     /locus_tag="ACHU2L_000460"
     CDS             complement(20857..21426)
                     /gene="ubiX"
                     /locus_tag="ACHU2L_000460"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR1355459.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21521..23038)
                     /gene="purF"
                     /locus_tag="ACHU2L_000461"
     CDS             complement(21521..23038)
                     /gene="purF"
                     /locus_tag="ACHU2L_000461"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR1355460.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23075..23563)
                     /gene="cvpA"
                     /locus_tag="ACHU2L_000462"
     CDS             complement(23075..23563)
                     /gene="cvpA"
                     /locus_tag="ACHU2L_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR1355461.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23822..24484)
                     /gene="dedD"
                     /locus_tag="ACHU2L_000463"
     CDS             complement(23822..24484)
                     /gene="dedD"
                     /locus_tag="ACHU2L_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR1355462.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24474..25742)
                     /gene="folC"
                     /locus_tag="ACHU2L_000464"
     CDS             complement(24474..25742)
                     /gene="folC"
                     /locus_tag="ACHU2L_000464"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR1355463.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25812..26726)
                     /gene="accD"
                     /locus_tag="ACHU2L_000465"
     CDS             complement(25812..26726)
                     /gene="accD"
                     /locus_tag="ACHU2L_000465"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR1355464.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26882..27541)
                     /locus_tag="ACHU2L_000466"
     CDS             complement(26882..27541)
                     /locus_tag="ACHU2L_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1355465.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27624..28436)
                     /gene="truA"
                     /locus_tag="ACHU2L_000467"
     CDS             complement(27624..28436)
                     /gene="truA"
                     /locus_tag="ACHU2L_000467"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR1355466.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28436..29449)
                     /locus_tag="ACHU2L_000468"
     CDS             complement(28436..29449)
                     /locus_tag="ACHU2L_000468"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1355467.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29515..30651)
                     /gene="pdxB"
                     /locus_tag="ACHU2L_000469"
     CDS             complement(29515..30651)
                     /gene="pdxB"
                     /locus_tag="ACHU2L_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR1355468.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30750..31745
                     /gene="flk"
                     /locus_tag="ACHU2L_000470"
     CDS             30750..31745
                     /gene="flk"
                     /locus_tag="ACHU2L_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR1355469.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31742..32920)
                     /locus_tag="ACHU2L_000471"
     CDS             complement(31742..32920)
                     /locus_tag="ACHU2L_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1355470.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33175..34416)
                     /gene="fabB"
                     /locus_tag="ACHU2L_000472"
     CDS             complement(33175..34416)
                     /gene="fabB"
                     /locus_tag="ACHU2L_000472"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR1355471.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            34575..36581
                     /gene="mnmC"
                     /locus_tag="ACHU2L_000473"
     CDS             34575..36581
                     /gene="mnmC"
                     /locus_tag="ACHU2L_000473"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR1355472.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36702..36980)
                     /locus_tag="ACHU2L_000474"
     CDS             complement(36702..36980)
                     /locus_tag="ACHU2L_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR1355473.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(37014..37562)
                     /gene="epmC"
                     /locus_tag="ACHU2L_000475"
     CDS             complement(37014..37562)
                     /gene="epmC"
                     /locus_tag="ACHU2L_000475"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR1355474.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37562..38371)
                     /locus_tag="ACHU2L_000476"
     CDS             complement(37562..38371)
                     /locus_tag="ACHU2L_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR1355475.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38371..39195)
                     /gene="mepA"
                     /locus_tag="ACHU2L_000477"
     CDS             complement(38371..39195)
                     /gene="mepA"
                     /locus_tag="ACHU2L_000477"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR1355476.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39199..40284)
                     /gene="aroC"
                     /locus_tag="ACHU2L_000478"
     CDS             complement(39199..40284)
                     /gene="aroC"
                     /locus_tag="ACHU2L_000478"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR1355477.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40319..41251)
                     /gene="prmB"
                     /locus_tag="ACHU2L_000479"
     CDS             complement(40319..41251)
                     /gene="prmB"
                     /locus_tag="ACHU2L_000479"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1355478.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41417..41968
                     /gene="smrB"
                     /locus_tag="ACHU2L_000480"
     CDS             41417..41968
                     /gene="smrB"
                     /locus_tag="ACHU2L_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR1355479.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42072..42962)
                     /locus_tag="ACHU2L_000481"
     CDS             complement(42072..42962)
                     /locus_tag="ACHU2L_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR1355480.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(42949..43473)
                     /locus_tag="ACHU2L_000482"
     CDS             complement(42949..43473)
                     /locus_tag="ACHU2L_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1355481.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43470..43940)
                     /locus_tag="ACHU2L_000483"
     CDS             complement(43470..43940)
                     /locus_tag="ACHU2L_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1355482.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(43937..44485)
                     /locus_tag="ACHU2L_000484"
     CDS             complement(43937..44485)
                     /locus_tag="ACHU2L_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1355483.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44490..45212)
                     /locus_tag="ACHU2L_000485"
                     /pseudo
     CDS             complement(44490..45212)
                     /locus_tag="ACHU2L_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(45232..47872)
                     /gene="yfcU"
                     /locus_tag="ACHU2L_000486"
                     /pseudo
     CDS             complement(45232..47872)
                     /gene="yfcU"
                     /locus_tag="ACHU2L_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(47954..48493)
                     /locus_tag="ACHU2L_000487"
     CDS             complement(47954..48493)
                     /locus_tag="ACHU2L_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1355484.1"
                     /translation="MAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIVPDDHKL
                     EVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYTIF
                     NTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFNAWLVGAA
                     DAPDLGNFEANTTFQITYL"
BASE COUNT        12025 a        13039 c        11968 g        11462 t
ORIGIN      
        1 ccttaccccc attgcgacac ctcggaaatc atcacgctgt taaactctcc gtaatgcgga
       61 aatatcatca tcgcgtttca ttagtgaatt cttaaatgag gcattttcac acaattatct
      121 tacagataaa aaaaccagac ttgcaattaa gaattagaac aattcactat ataacattgc
      181 atgtaaagca tataagccta attatttgtc attattaagt tattaacagc acaatcgagc
      241 cttcccctct ggcaaaatct tattctgcag accttcaaaa caccgtcctg ggggagtaca
      301 ttgttctaag ctgacttcca cggcagggag tggcgataac agcaaaaaag gtcaagattc
      361 atgtccccca ttgaaaaatc cagcaaatta gagaatgtct gttatgacat ccgtggtccg
      421 gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa actgaacatc
      481 ggcaacccag ccccattcgg ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc
      541 aacctgccta ccgctcaagg gtattgcgat tccaaagggc tttactccgc gcgtaaagcc
      601 atcatgcagc actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc
      661 ggcaatggtg tatcggagct tatcgttcag gcaatgcagg cattgctgaa cagcggggac
      721 gaaatgttgg ttcctgcacc agattaccca ctctggaccg cggcggtttc gctttccagc
      781 ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact ggttcccgga cctcgatgat
      841 attcgcgcta aaattacgcc tcgtacgcgt gggattgtta ttatcaaccc aaataaccca
      901 accggcgcgg tatattccaa agagctttta atggagattg tggagattgc acgtcagcat
      961 aatctcatta tcttcgccga tgaaatttat gacaaaattc tctacgacga tgctgagcat
     1021 cactcaattg cgccgctggc acctgacctg ctgaccatta cctttaacgg actgtcgaaa
     1081 acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac
     1141 gccaaaggct acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt
     1201 cctgcgcaac acgccattca gaccgcgctg ggtggttatc agagcatcag tgaatttatt
     1261 acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt gggaactgat caacgatatt
     1321 ccgggcgttt cctgcgtgaa acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc
     1381 aaacgcttta acattcatga cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa
     1441 gttctgttgg tgcaagggac ggcattcaac tggccgtggc cggatcactt ccgcattgtc
     1501 acgctaccgc gtgtcgatga tatcgagctg tctttgagca agttcgcgcg tttcctttct
     1561 ggttatcatc agctgtaatc ttaatttcac tgccggagat tgcatccggc agtgttatcc
     1621 cgccacaatg acctgatgat gtcatcatac gtaaggtcac tatgaaacag agccattttt
     1681 ttgcccatct ctcccgcctg aaactcatta accgctggcc gctaatgcgc aacgtgcgga
     1741 cggaaaatgt gtccgaacac agtttgcagg tagcgatggt cgcccatgcg ctggcagcta
     1801 tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt
     1861 accacgatgc ctcagaagtg ctcaccggcg atctccctac tccggtgaaa tacttcaatg
     1921 cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc tcagcagaaa ctggtcgata
     1981 tggttccgga agaactgcgg gatatctttg cgccgttaat tgacgagcat gcatatagcg
     2041 atgaagaaaa atcgctggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg
     2101 aagaactcgc ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc
     2161 ttgaagcgcg tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc
     2221 atctttcgct cgatgagatt agccaggatt caccgctgta agcagccgga gtctgcgtcg
     2281 catcagtcaa taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg
     2341 gatcatcacc acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa
     2401 gtcactgaag ctgtaatttc ccggacctaa aaccagggtg ttaacaggtg aagacaccgg
     2461 tgtcataaag gcggcggatg ctgccatcgc cacgaccatc gcaaatggat aaggcgacac
     2521 tcccatcgtt ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt
     2581 agagataaac agcccaataa ccgccgacaa gacaaacaaa caccccagca tcatatgtgg
     2641 cccataaccg ccgccaatat ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt
     2701 ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat
     2761 ggatttatag gcgctttcag catctataca gcggaatttc cccatcagca ggcaggcgat
     2821 gatagcggca acaggattag gaatttcatt tgtcagcatt aacgccacca ttagcaccag
     2881 acagaaaatg gcatggggtg cctggctgtg cgcgggcgat gcttcactca cctcttccgg
     2941 taagttcagc gctacgaagt cgcggccctg tttggccagc ataccgatca gtttccagtt
     3001 acccacaacc aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag
     3061 cgccacgcca ttgcgcttga gccccaccac attcagtccg tagcgggtac gaaaaccaat
     3121 ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc
     3181 cacatcaagg gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg
     3241 ctcactacaa aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc
     3301 acgcgcgcga aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca
     3361 gcgttcaaca ccgatgacgt tagcgccata acgctcacgt aatttgagat catccagccg
     3421 ttgacctatc attggcgatc cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg
     3481 atattcacgg ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg
     3541 ggtatcccct ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac
     3601 gaccaggcca ataggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa
     3661 ttcactgttg actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag
     3721 cccggcaaaa cttaacggca tcatcagacg tgacggcgaa gtttgcatac gcatggcaac
     3781 gcttaacacc acgggaataa agatagcgac aacgctggtt gaactcataa acgcgccaag
     3841 ccccgcgacg gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgccacttt
     3901 gaccagccat gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat
     3961 aaacaaggcg gcaatcagga caacgttagg atcagaaaag ccggaaaata cttcggggac
     4021 cgtcagcgtt ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg
     4081 cactctgccc gtcgcaaaca agacgatggc aacggccagt aatgaaagaa cccaaatcaa
     4141 ttcaccgttc acaacttatc cttgttaatt gagggggatg acttgattct gccataaaaa
     4201 agccccgacg agacggggct aaatcatgat caggtgtttc actgaataat aacatcgcca
     4261 tttggctgtt tggtcacagt aatttgctcc agactgtgga ggaccaaatc gacctcattc
     4321 aggcgcgggg tatctgccgg agcgttaacc gcaatgacat ggcaacccgc cgccaggcca
     4381 gaaagcacgc cagcgggagc atcttccacc accacacact cctgcggcgc aagccccagc
     4441 agctgcgcac ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg
     4501 gttacaaaca cctctggtgc gggaagcccc gctattttat ggcgcgctcg cgctaccggc
     4561 atggagccag aagtcacaat ggcccacgga atacctgctt tattcaaatg attgagtaag
     4621 gcgattgccc caggaagcgc ggtaatacct tcggtttccg tggcctcgat ttgctccaga
     4681 cgcgtaaact cggcggcaat atcagcctcg gatttgcccg ccataaaatg gcgcaaagag
     4741 gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa cccatgacgt
     4801 ctggcccagt tgctccacgc ccgttctacc gcaggcaggg aatccaccag cgttccatca
     4861 agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct caggcgttga taatttgatt
     4921 aatttcgttg gcgctcaaat ggtactgacg cgggcaggca tgccacacat taagcatgcg
     4981 ctgatatttt tcccacattg gcgtctgggc gttaaaacca tgagtcccag cgtcaaaatg
     5041 ggtatagcgc ccttccacat taaccataaa gcggacataa ccgaggtaac gtgcttcagt
     5101 ggcggcgtca aagccgagga aggtgacacg acgttcatcg atggattgct ggtcctgcaa
     5161 attagaccaa gaaacataca acgcatgata catatccata atgtcgatga tagtgcggca
     5221 ggtttcttct ttcagctcgc caaactcgcg atccaattca cgcatctgta atccgtaacc
     5281 acgctcaata attgtttgca ggcgacggta acgttcagca tttgccggat cgagcatagt
     5341 catcatcttg tactgattag acaaaataag acgttgcgcg ttggtcattt ccattgttga
     5401 ctcctgtatc actctactac ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg
     5461 cgtaatcagg ggtcaattac aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc
     5521 gcttaagcgt gtcagctaat gcctggtaat caggcttgcc ttcaacgggt gccaacacct
     5581 gtccagactc ctgcaattta ccgcgaactt cataaaacca gttaaggatt gcagggggta
     5641 atggcgttac agaacgcttg cccagccacc acaatccctg catgggtaaa cttaaggcga
     5701 acagtgcagt ggcaactgcc ggcccaagca gaccgcccag ggcaatctgc cagcagagag
     5761 taaatacggc gatcggcggc ataaaacgga tcgcataacg cgtcatcttg ataacgcgat
     5821 tttcgacaaa gaccggagca aggcgttttt ccagtggcca cgtctttgag taatgctgtc
     5881 cgcggcgaaa caagctaaaa aaattaacag aacgattatc cggcgttgac atgcttcacc
     5941 tcaacttcac atataaagat tcaaaaattt gtgcaaattc acaactcagc gggacaacgt
     6001 tcaaaacatt ttgtcttcca tacccactat caggtatcct ttagcagcct gaaggcctaa
     6061 gtagtacata ttcattgagt cgtcaaattc atatacatta tgccattggc tgaaaattac
     6121 gcaaaatggc atagactcaa gatatttctt ccatcatgca aaaaaaattt gcagtgcatg
     6181 atgttaatca taaatgtcgg tgtcatcatg cgctacgctc tatggctccc tgacgttttt
     6241 ttagccacgt atcaattata ggtacttcca tgtcgagtaa gttagtactg gttctgaact
     6301 gcggtagttc ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa gagtaccttt
     6361 ctggtttagc cgaatgtttc cacctgcccg aagcacgtat caaatggaaa atggacggca
     6421 ataaacagga agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg
     6481 ttaatactat tctggcacaa aaaccagaac tgtctgcgca gctgactgct atcggtcacc
     6541 gtatcgtaca cggcggcgaa aagtatacca gctccgtagt gatcgatgag tctgttattc
     6601 agggtatcaa agatgcagct tcttttgcac cgctgcacaa cccggctcac ctgatcggta
     6661 tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa aaacgttgct gtatttgaca
     6721 ccgcgttcca ccagactatg ccggaagagt cttacctcta cgccctgccg tacaacctgt
     6781 acaaagagca cggcatccgt cgttacggcg cgcacggcac cagccacttc tatgtaaccc
     6841 aggaagcggc aaaaatgctg aacaaaccgg tagaagaact gaacatcatc acctgccacc
     6901 tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa atgcgttgac acctctatgg
     6961 gtctgacccc gctggaaggt ctggtcatgg gtacccgttc tggtgatatc gatccggcga
     7021 tcatcttcca cctgcacgac accctgggca tgagcgttga cgcaatcaac aaactgctga
     7081 ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag cgactgccgc tatgttgaag
     7141 acaactacgc gacgaaagaa gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg
     7201 cgaaatacat cggtgcctac actgcgctga tggatggtcg tctggacgct gttgcattca
     7261 ctggtggtat cggtgaaaat gccgcgatgg ttcgtgaact gtctctgggc aaactgggcg
     7321 tgctgggctt tgaagttgat catgaacgca acctggctgc acgtttcggc aaatctggtt
     7381 tcatcaacaa agaaggtacc cgtcctgcgg tggttatccc aaccaacgaa gaactggtta
     7441 tcgcgcaaga cgcgagccgc ctgactgcct gatttcacac cgccagctca gctggcggtg
     7501 ctgttttgta acccgccaaa tcggcggtaa cgaaagagga taaaccgtgt cccgtattat
     7561 tatgctgatc cctaccggaa ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg
     7621 tgcaatggaa cgcaaaggcg ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac
     7681 cggtggcgat gcgcccgatc agactacgac tatcgtgcgt gcgaactctt ccaccacgac
     7741 ggccgctgaa ccgctgaaaa tgagctacgt tgaaggtctg ctttccagca atcagaaaga
     7801 tgtgctgatg gaagagatcg tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt
     7861 tctggttgaa ggtctggtcc cgacacgtaa gcaccagttt gcccagtctc tgaactacga
     7921 aatcgctaaa acgctgaatg cggaaatcgt cttcgttatg tctcagggca ctgacacccc
     7981 ggaacagctg aaagagcgta tcgaactgac ccgcaacagc ttcggtggtg ccaaaaacac
     8041 caacatcacc ggcgttatcg ttaacaaact gaacgcaccg gttgatgaac agggtcgtac
     8101 tcgcccggat ctgtccgaga ttttcgacga ctcttccaaa gctaaagtaa acaatgttga
     8161 tccggcgaag ctgcaagaat ctagcccgct gccggttctc ggcgctgtgc cgtggagctt
     8221 tgacctgatc gcgactcgtg cgatcgatat ggctcgccac ctgaatgcga ccatcatcaa
     8281 cgaaggcgac atcaatactc gccgcgttaa atccgtcact ttctgcgcac gcagcattcc
     8341 gcacatgctg gagcacttcc gtgccggttc tctgctggtg acttccgcag accgtcctga
     8401 cgtgctggtg gccgcttgcc tggcagccat gaacggcgta gaaatcggtg ccctgctgct
     8461 gactggcggc tacgaaatgg acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac
     8521 cggcctgccg gtatttatgg tgaacaccaa cacctggcag acctctctga gcctgcagag
     8581 cttcaacctg gaagttccgg ttgacgatca cgaacgtatc gagaaagttc aggaatacgt
     8641 tgctaactac atcaacgctg actggatcga atctctgact gccacttctg agcgcagccg
     8701 tcgtctgtct ccgccagcgt tccgttatca gctgactgaa ctggcgcgca aagcgggcaa
     8761 acgtatcgtt ctgccggaag gtgacgaacc gcgtaccgtt aaagcagccg ctatctgtgc
     8821 tgaacgtggt atcgcaactt gcgtactgct gggtaatccg gcagagatca accgtgttgc
     8881 agcgtctcag ggtgtagaac taggtgcagg cattgaaatc gttgatccag aagtggttcg
     8941 cgaaagctat gttggtcgtc tggtcgagct gcgtaagaac aaaggcatga ccgaaaccgt
     9001 tgcccgcgaa cagctggaag acaacgtggt gctcggtacg ctgatgctgg aacaggatga
     9061 agttgatggt ctggtttccg gtgctgttca cactaccgca aacaccatcc gtccgccgct
     9121 gcagctgatc aaaactgcac cgggcagctc cctggtatct tccgtgttct tcatgctgct
     9181 gccggaacag gtttacgttt acggtgactg tgcgatcaac ccggatccga ccgctgaaca
     9241 gctggcagaa atcgcgattc agtccgctga ttccgctgcg gccttcggta tcgaaccgcg
     9301 cgttgctatg ctctcctact ccaccggtac ttctggtgca ggtagcgacg tagaaaaagt
     9361 tcgcgaagca actcgtctgg cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct
     9421 gcagtacgac gctgcggtaa tggctgacgt tgcgaaatcc aaagcgccga actctccggt
     9481 tgcaggtcgc gctaccgtgt tcatcttccc ggatctgaac accggtaaca ccacctacaa
     9541 agcggtacag cgttctgccg acctgatctc catcgggccg atgctgcagg gtatgcgcaa
     9601 accggttaac gacctgtccc gtggcgcact ggttgacgat atcgtctaca ccatcgcgct
     9661 gactgcgatt cagtctgcac agcagcagta atctcgtcat catccacagc tttgcgctgc
     9721 ggatatctga accggaaata atcactattt ccggtttttt attctcttaa tttgcattaa
     9781 tcctttctga ttatcttgct taactgcgct gcatcaatga attgcgccat ctcactttgc
     9841 atacttacca ctttgttttg tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc
     9901 caaaccaaca agaagatggg caatgcccga tacgttggtg attatctttt ttgttgctat
     9961 tttaaccagt cttgccacct gggtagttcc ggtggggatg tttgacagtc aggaagtgca
    10021 gtatcaggtt gatggtcaaa caaaaacacg caaagtcgta gatccacact catttcgcat
    10081 tctgactaac gaagcaggcg aacctgagta tcaccgcgta cagctgttca cgacgggcga
    10141 tgaacgcccg ggcctgatga acttcccgtt tgaagggtta acctcaggat cgaaatacgg
    10201 gacagccgtt ggcatcatca tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat
    10261 gcgtacagga accattgata acggtatcct ggcgcttatt cgccataccc gcgggaatga
    10321 aattctcttt attcctgcgc tgtttattct gttttcactt ggcggcgcgg tatttggtat
    10381 gggagaagag gccgtcgcct ttgccattat catcgcaccg ctaatggtcc ggctgggcta
    10441 tgacagtatt accaccgtcc tggtgactta tattgccacg caaatcggtt ttgccagttc
    10501 gtggatgaac ccgttttgtg tggttgttgc tcaggggatt gccggcgttc cggtgctttc
    10561 tggctccggg ttgcgcatcg tggtgtgggt tatcgccact ctgattggcc tgatctttac
    10621 catggtgtac gcctcacgag tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga
    10681 ccgcttcttt cgtgaaaagc aggcggatgt tgaacaacgt ccgtttacct ttggtgactg
    10741 gctggtattg attgtcctga cagccgtaat ggtctgggtg atttggggcg tgatcgttaa
    10801 tgcctggttt attccagaaa ttgccagcca gttcttcacc atgggtctgg tgattggcat
    10861 catcggcgtt gttttccgcc ttaacggcat gacggttaat accatggctt catcctttac
    10921 cgaaggggcg cgaatgatga tcgcccctgc cctgctggtg ggtttcgcca aagggatttt
    10981 gctgctggtt ggtaatggtg aagcaggtaa tgccagcgtg ttaaatacta tcctcaacag
    11041 cattgccaat gccattagcg gtctggacaa cgcggtcgcg gcctggttta tgttgctctt
    11101 ccaggctgta tttaatttct tcgtgacgtc cggttctggt caggcggcat taactatgcc
    11161 gttactggca ccgcttggcg atctggtcgg tgttaaccgt caggttaccg tgctggcgtt
    11221 ccagtttggt gatggcttca gccacatcat ttacccaacc tcagcttcgt taatggcaac
    11281 gctcggtgtg tgccgggtgg acttccgtaa ctggctgaag gtgggcgcga ccctgcttgg
    11341 actgctgttt attatgtcca gcgtcgtagt gatcggcgct cagttgatgg gctaccacta
    11401 aaaatgttaa gagccgcaat tgcggctctt ttttcattct gccgtttcag tctctacggc
    11461 ttcatttttg gcattgcgct tcatccacaa cgccagcgct ttcagcgagt ctggagtgaa
    11521 ctcatcgcag cgtgcggtga tttcttccgg cgtcagccag caaactccac tgacttcatc
    11581 ttcctgtagg gcgaagggac cgtgagagac gcaactgaac aacgcgcccc agacacggca
    11641 atttttatct tcgaaataga actgcccgtg ctcggcaaag gggacaccgg caatgcccaa
    11701 ctcttcttcc gcttcgcgac gcgcggattc cagcagttgc tcatcggcct gaactacacc
    11761 gcctgcggtc gcatctaaca tgccgggtaa aaagtctttt gtctcggtac gacgctggac
    11821 cagaattttg cccatgccat catgcacgac gatgtaagtt gcacgatgac gcagacactg
    11881 tgcccgcatt tgttcccggc tggcttgtgc aatgacttcg ttctcttcat tgacaatatc
    11941 cacccattca gtacttgcca aacgacgctg ttccattatc aggaaacctt ctttttctgg
    12001 cgctcttacg gcgcattttg agttgtgggt aaattacgga ttaatcgcga cctgcgcaat
    12061 gatactttga tcattgagtg cgattacact aagtacgtca ttatccagca tgccataacc
    12121 cgccggatta ccgcctttcg gaatactcac cgagccgggg ttgaagtgga aaatctcccc
    12181 ccgctgttct gccactggca gatgggtatg accgtacacc agcacatcgt tctggtttaa
    12241 agcaggtaga ttttccgggc caaaaagatg accatgcgtc aaaaacagac gttgtttttc
    12301 cagtaatacc tgttgccacg gcgcggttat cgggaaatgc agcatcattt gatccacttc
    12361 gctgtcgcag ttgccacgca cagcgataac cttatgtgcc acttcattaa gccgttcagc
    12421 gactttggct ggcgcgtaac cctccggtaa agcattacgc gggccgtgat tcaacacgtc
    12481 gccaaggatc accagccact gggcaccgct ttggacaaac aactccacaa cacgttccgt
    12541 cgccggtaac gacccatgaa tgtccgatgc aaacatcagt ttcatcactc actcctcgtc
    12601 gaaaaaacgt aagctatgat actggattct gtcgttgcta tcagccagat tgcttcgccg
    12661 aaagtgcaat aaaacgctgg acagacgctc gctgccactg gaatgtcgca taatccacca
    12721 ggcgttctgg cacctcatcg ccatgtagta ccaggcggtt aatcattagc gccagatcag
    12781 tatcagcaat gcaccattca ccaaataaat tcggctgacc cagtgctaac aaatgttctg
    12841 ccatcgcgaa cagtttctct gcactggctt ttccctcggc cgttagtggc gcttttttcg
    12901 cccccgcaaa gacaacatcc gtcggacgct cttcgcggat gggcatcaga tcgctgcgca
    12961 gccaggcctg aatctgtcgt gcacgcgcac gattttctaa atcaagcgga taaatacgtt
    13021 cccaggtcgg tggcgcaaat cgatcttcca gatactccgc aatggcagaa gattcactca
    13081 actcaaaatc atcgatttgt aataacggca cacggcgtgt ctgaccgtaa ccttgccacg
    13141 tcggctgcaa atgttcaccg ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt
    13201 tttcctgcaa cgccacccag gcggataaca cataagggga gaaaaagtgg gcatctgacc
    13261 aaagcgtgat agcgggttta ctcattatgt cctcggcaat aaagggggct ggctatcaaa
    13321 ctatagagcc tttttgtcac tgtcacgtga cgaatactca cgtaagtcgc cgcgctttct
    13381 atacttgtgg tgttcagatg attaaacaac cggagctgca atgatcgatc tctatttcgc
    13441 cccgacaccc aatggtcaca aaattacgct gtttctcgaa gaagcagagc ttgattatcg
    13501 cttgattaag gtagacttgg ggaaaggcgg tcagtttcgt ccggaatttt tgcgcatttc
    13561 gcccaacaac aaaattccgg caattgttga tcattctcct gccgatggcg gcgaaccgtt
    13621 aagcctcttt gaggctgctg ccattttgtt gtatctggct gagaaaacag gactcttttt
    13681 gagtcatgaa acgcgtgagc gcgccgccac attacagtgg ttattctggc aggtaggcgg
    13741 actggggccg atgcttgggc aaaatcatca ttttaatcac gcagcccccc aaaccattcc
    13801 ttacgctatt gaacgttatc aggttgaaac tcagcgtctt taccatgtac taaacaagcg
    13861 gctggaaaac tcgccctggc tgggaggcga gaactacagc attgcggata ttgcctgctg
    13921 gccgtgggtt aatgcctgga ctcgcctgcg aattgacctc gcaatgtatc cggcagtcaa
    13981 gaactggcat gagcggatcc gttcgcgccc tgccaccggg caggcactgc taaaagcaca
    14041 actcggtgat gagcgttcgg atagttaaca gaaacaggtt ctcgtgtatt atttcatcct
    14101 aagtaaaaca acggagaacc tgcaatggca caacctgccg ctattattcg tataaagaac
    14161 cttcgtttgc gtacgtttat cggaattaag gaagaagaaa ttaacaaccg tcaggatatt
    14221 gttatcaatg tgacgatcca ctaccccgcc gataaagcgc gcactagcga agatatcaac
    14281 gatgcgctga attatcgcac cgtaacgaaa aacattattc agcatgtaga gaataaccgt
    14341 ttctctttgc tggaaaaatt aactcaggat gtgctcgata tcgcacgtga acatcactgg
    14401 gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta
    14461 tccatgacct taagctggca gcgttaatcg ccatatcggg aggctgcatg aacatagtga
    14521 tcaccggagg gacgggatta attggtcgcc atttgattcc gcgtttgctg gagctgggcc
    14581 atcaaattac ggtagtgacg cgtaacccgc agaaagccag ttccgttctc ggccctcggg
    14641 tgacactatg gcaagggctt gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca
    14701 acctggccgg agaaccgatt gctgataaac gctggactca caagcaaaaa gagcgtctct
    14761 gccaaagccg ctggaatatc acgcaaaaac tggtcgattt gattaatgct agcgacacgc
    14821 cgccgtcggt actcatttcc ggctcggcaa cgggctatta tggcgactta ggtgaagtgg
    14881 tggttaccga agaggaaccg ccgcataacg aatttactca taaactctgc gcccgctggg
    14941 aagagattgc ctgccgggcg caaagtgaca aaacgcgagt gtgcctgctg cgaaccggcg
    15001 tggtactggc accggacggc ggtattctcg gtaaaatgct gccaccgttt cgtcttggcc
    15061 tgggcgggcc gattggttcc ggtcggcagt atctggcctg gattcatatt gatgatatgg
    15121 tcaacggcat tctctggctg ctggataacg agctgcgcgg gccatttaat atggtttcgc
    15181 cctacccggt acgcaatgaa caatttgccc atgcgctcgg tcatgcgctg catcgcccgg
    15241 ctattttgcg cgtccccaca accgccattc ggctgttaat gggcgaatct tcagtactgg
    15301 tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga agcgggtttt gcgtttcgct
    15361 ggtacgattt agaagaggcg ctggcggatg tcgttcgctg atgtggttta cagcaaacat
    15421 ccgccagtta actccctgtg ttacaggatt agtggctttg cgcgataaga tcgtctggtg
    15481 aaagtcgggt caccatcata actaactctc tgtctaaacc tctatccagc atctcctgag
    15541 caatacgcag ggcttcttcg tgtttgccct gcattgcgcc ttcttcacgt aatctgtcag
    15601 caatggtcat cagtttctcc ttttcttgtg gtgcgcgttc tgttatctca ccaataaatg
    15661 cacggaaacg ctgggcatcc cctgtttgta atacgtaatt aaacagggct tttagctgtc
    15721 tgtcattagt gttccctgta actaacagcg aaacaatttg gtcgactaat cccaacagat
    15781 cgcgctgacg aatatgtttc tgaattaact ccaacagcgc cattttgcgg tgttgcataa
    15841 tctcgtcatc cggcaccacg gtaatatcca ccaacggaaa cgctgatgaa tatattttgc
    15901 gggctatagc aggctcggca aattcatcaa gccagcagag tgaataagga taaggacttc
    15961 tgcaaccatg ataaaacagc atcgggatca ccaatggaag ctctttatag cccgcatcaa
    16021 gatggttttg cattgccgca atggaataac gcatcatgcg aaaagccatt aattcttccg
    16081 gcttactttg gtgctctatc actacataaa tataacccac tccctcctgc gttttcacag
    16141 accacaagag gtcggaataa tattgccgca ggtcttcatc aataaaactg tttggttcta
    16201 gtttaagcgt cgttaaatca cacagtttgc gcagcggcgc gggaagatga atattaataa
    16261 aattccgcgc ggtgtctgga tggcgtaaaa aagatttaaa taccgcatca tgcggcgtgg
    16321 aagttgtcga tattgtcatg gcgttccgtc acccctcaaa tttaagatga cgcgacaata
    16381 accacgatta acacccaatg cagcaggcag tttttatctt tggacagcga attcagaagt
    16441 atttttagcg gactgcaact tttacatttc agcggaagct gactcgcaaa ttccccttga
    16501 aaatactccg gcagcgttag ccaccggaga ggaaattatt tcagcgatcc cttaaggaac
    16561 tgttgcagac gagggctttg cgggttgcca aataactgct ccggcgcgcc ctcttcttct
    16621 attttcccct gatggaggaa aatgacgtga gtagaaacat ggcgagcaaa gcccatttcg
    16681 tgagtcacta ccaccatggt tttcccctct tctgccagtt gctgcataat acgtaacact
    16741 tcgcctacca gttcaggatc gagcgccgag gtaggttcat caaacagtaa aacttctggt
    16801 tccatcgcca gcgcccgcgc gatagaaaca cgctgttgct gaccgccgga aagatgcacc
    16861 ggatatttcc cctgcgcacg ttcgtctatc ccgactttcg ccagatactt caccgcccgc
    16921 tcgcgcgctt cctgcttgct caggcccaac acctgaatcg gcgcttccat gacgttttcc
    16981 aacaccgtca tatggctcca gagattgaag tgctggaata ccatcgtcag gcgtgtgcgc
    17041 agtaagcgca gttgattttt atcggcgact ttgagttgac cgtctttgtc gcgcaccaga
    17101 ttgatcgtct ggccattgac cacgatcgat ccttcactcg gtttttcgag gaagttaatg
    17161 cagcgcagaa aggtactttt ccccgatccc gacgatccga tgatgcttat tacatctccg
    17221 gcattcgctt gcagtgagac ccctttcagc acttcgtgtt cgccatagcg tttgtgcaaa
    17281 tcgataacgt ttaatttatt ctcggacatc gtattctcag tgcgttgaag aaggtttcac
    17341 atgctgcaac cagcgttttt ccgctctgcg aaagaggctg atcaggacat aagagatgat
    17401 taaatagagc accgcggcaa tgccgaaggc ggtaaaaggt tgatacgtgg cggcgttaat
    17461 atcgcgggct attttcagca gatccggcac agtggccgta aacgccagcg cagtagagtg
    17521 cagcatcagg atcacttcgt tgctgtatgc cggtaacgca atacgcagcg cagaaggcaa
    17581 aataatgcag cgatacattt taaaggtcga gaagccatag gctctggcgg cttcaatttc
    17641 cccatgcgga accgaacgga ttgccccagc aaaaatctcg gtagtgtaag cgcaggtgtt
    17701 aagcgtcagc gccagcacgg tacagttcag gccactgcgg aagaaagcgt taaggaattc
    17761 ggttccctta acaatctcaa gcgtgtacat gccggaatag aacaccagca actgaacata
    17821 cagcggcgta ccgcgaaaaa tataggtaaa taaccagatt ggaaactgga tgtatttatt
    17881 actggagaca cgaccaatcg ccagaaacag cgccaggact ccgcctatca ctaccgacaa
    17941 aataagcagc cacagagtga tcgccacacc agtaaagcga taaccgtcgg tccacagcag
    18001 cggtttccag tattcatgta agatttcgat cacaggtcag ccctcttcac acccacggag
    18061 tagcggcgct caaggaacag cagcacacca ttggaaacgg tggtgaaaac caggtaaatc
    18121 acgccacaga cgatggcgaa atagaacggt tcccaggtac ttttgcctgc cagctgcgtg
    18181 gctttgacca catcttccag gccgagtaac gaaaccagtg cggtagattt gaggatcacc
    18241 tgccagttgt tgccaatgcc tggcagggcg taacgcatca tcgccggaaa catgatccgc
    18301 cgaaacactt gcccgcgagt aaaaccgaac gccgtcgccg cctctatatg ccctttcggc
    18361 actgccataa acgcgccacg gaacgtttcg gtaaaataag caccgtagat aaaaccgaga
    18421 gtgataatac cagcgaccat cggatcgata tcaatctgcc cgacgcccat cgcctccgtc
    18481 accatgttta gcgcaatctg caaaccatag aaaatcagca gcatcaacac caagtccggc
    18541 acgcctcgaa tcagcgtggt gtacccttcg aaaatcagcc ccgaaagccg attttgcgag
    18601 agcttaccgc cagcgccaat taaaccgatg attacagcga gcactacaga gctgatagcc
    18661 agctccagcg tgacgagcgc accctgtaaa ataacacctg aaaacccata caacatgctg
    18721 cctgtcctgt cgtgtgtggt gaattaccgc ctcttgtctc cctccggtta cccgaaggga
    18781 gcgggctttc tttcaccgat ggcgattaac caccataaac atcaaaatcg aagtactttt
    18841 tcgctaattt ctcgtaagta ccgtcagcgc gcatttcggc aaaggctttg ttcagtgcct
    18901 cgcgcagttc gttatcttct ttacgcaggc ccatgccggt ccctacgcca aacagttttt
    18961 catctttaac agacggacca ccgaatttgt aatctttacc gacgggttgt ttgaggaaac
    19021 cttcgctggc agcgacctca tcctggaacg cggcatcaat acgtccggca gtcaggtcag
    19081 aataaatgtt gtcctgcccc tgatacgaga cgatttcaat gccttttggt gcccagtgtt
    19141 cattaccgaa cgtctcctgg gtggtgccct gcaatacgcc tacccgtttg cctttcagcg
    19201 actcgactgt cggctgaatg tcagaatttt tcgccaccac cagacgagaa tcggcagcgt
    19261 acagtttgtc ggtgaaggca atttcttgct gacgtttttc cgtaatggaa agcgatgaca
    19321 tgatggcgtc aatcttcttc gcttttaagg acgggattaa cgcatccagc gggttttcga
    19381 caaacgtaca ttgcgtattg atgcgcttgc acaactcctt cgccagctcg atatcgaaac
    19441 caaccagttc gccttgtgaa ttttttgatt caaatggcgc ataggtcggg tcggtaccga
    19501 tgcggatgtt ttgcggaatt gcagcaagtg ccgcagttgc gctggagaag gccagaacca
    19561 gagagagcga tagcaccagt tttttcataa tgtcctcaac tgacagtctt tttataggga
    19621 atatttacag gtttgttaca cttatcgtgc cataaaattg accatcaagg caaaatatcc
    19681 cgccccgtta gacgtattat ttttcattaa aaatgcttaa gtatgcattt caatgcacca
    19741 cgatagtgca acaaacctgg cgtgcaccat gatgatcatt ccatcaggta cagcttccca
    19801 gcgacgtatc agtcaccgta gacattaaag tcgaaatact ttttcgccat cttgtcgtag
    19861 gtgccgtcct gacgcagctc gccaagcgcc ttattgaagg cagccgtcag ttcagcataa
    19921 tctttacgta gccctacacc ggtgccatca ccgaagtatt ttttgtcttt tactgatgag
    19981 ccagcaaaag cgaaatcttt accagcaggt tgcttgagga atccttcgct ggcagcaact
    20041 tcatcttgta acgcagcatc cagacgtcct gcagccagat cggaatagac caaatcctgg
    20101 ttggcatagg ccaccacatc cacgccttta ctacgccagg tctcgttagc gtaagcttcc
    20161 tgggttgatc cctgcagcac accaacatgt ttacctttca gtgaatccag cgttggctga
    20221 atcggtgaac ctttggcagc aatcaaacga gaatctgcgg cgtacagctt gtcggagaag
    20281 gcaatctcct gctgacgttt atcggtaatg gaaagcgacg aaataatagc gtcgattttt
    20341 ttcgctttca gtgaggggat cagcgcgtca aagtcactgg caacccaggt acatttcacc
    20401 tgcatccgtt tgcacatctc gttaccgaga tcgatatcaa agccaacaaa atcaccttta
    20461 gcatctttcg acgagaacag cgcataggtg gtatcggttc cgatacgtac cgtctccggt
    20521 agcgccgcat agctggaagc cgctgcggag agaccgacta ataaagacag agcgagaatc
    20581 gacttcttca tacataaccc tcaagtgaaa atggcttttt tatgttgtgt attgtgttgt
    20641 gtgtttgcag gctctttcat gcaggtctta tgccatcttg acgacaacag taacattcaa
    20701 cgttaaatat gttaataaga cattgcatta ttgtcctgaa gttgaagata gcaggtatag
    20761 cggttgaatc gcagcgtttc gattgaatgg cagaaacaaa atgtcgagga tttgatcgcg
    20821 gttgcaaaat tgccctgaaa cagggcaaca gcggagttat gcgccctgcc agcgggcaaa
    20881 gagatcttca ggaagggtta ttgcaaactg gtcaagaaca cgattaaccg tctgatttat
    20941 cacatcatca agggattgcg ggcgatgata aaacgccgga acgggaggca taatcaccgc
    21001 accgatttct gccgcctgag tcattaaacg cagatggcct aagtgcaatg gtgtttcacg
    21061 cacgcagagc accaacgggc gacgctcttt cagcaccaca tctgccgcac gggtcagtaa
    21121 gccatcagta tagctatgga caatgccgga cagggttttg attgaacagg gtaaaatcac
    21181 catccccagc gtctggaaag aaccggaaga gatgctggcg gaaatatcgc gcgcatcgag
    21241 cgtgacatca gctaatgcct gcacttcgcg cagggaaaaa tccgtttcga gggataaggt
    21301 ctggcgcgcc gcctggctca tcaccagatg cgtttcgata tctgtgacat cgcgcagaac
    21361 ctgtaataag cgcacgccat aaatcgcgcc gctggcaccg ctgatgccta caatgagtcg
    21421 tttcataaat agttgccctg tcagacttgc aggcagactt tgcagtattt cggcgggagt
    21481 tgcaagtcag ggtgccagac cggcaccctc agcgaaggca tcatccttcg ttatgcattt
    21541 cgagattttc cacttcgttc tgacgttgca ctgctttggc gtcatcatta cgtaacgtat
    21601 cgaggaaatc gaggtagccc tgatcaacat ctttggtgac gtagacgccg ttgaacaccg
    21661 agcattcaaa ctgctggata tccggatttt cagcgcgaac ggcgtcgatc agatcgttca
    21721 gatcctggaa aatcaacccg tcagcaccga tgatctggcg aatttcatca acttcgcgac
    21781 cgtgagcgat cagttccgtg gcgctcggca tatcaatacc ataaacgttc gggaagcgaa
    21841 tttccggtgc cgcagaagcg aggtacactt tcttcgctcc ggcttcgcgt gccatctcga
    21901 taatctgctc agaagtggtg ccacggacga tggagtcgtc gaccagcagg acgtttttat
    21961 cgcggaactc ggcgcggttg gcattcagtt tacggcgcac ggacttacga cgcagctgct
    22021 ggcccggcat gataaaggtg cggccaacat agcggttttt aacgaagccc tggcggtacg
    22081 gtttgcccag aatacgagca atttccagcg cgatatcaca cgaggtttct gggatcggga
    22141 tcaccacgtc gatatccaga tcttcccatt cgcgggcaat tttctcgccc agtttcgtgc
    22201 ccatattcac acgcgcgctg taaacggaaa ttttgtcgat aaacgagtcc gggcgggcaa
    22261 agtatacata ctcaaacagg cacggattgc tgaccggatt gtcagcacat tgacgggtaa
    22321 acaactgccc ttcttcagtg atgtaaatcg cttcgcccgg cgcgacgtca cgcaggaaat
    22381 caaagcccag cgtatcgagc gctacgcttt cggaagcgac catatattct gtacggttct
    22441 cgtcaatatc acgttttccc agtaccagcg gacgaatccc gtttggatcg cggaaagcaa
    22501 ccataccgtg gccgataatc atcgccacac aggcatacgc gccgcggatt aagcggtttg
    22561 tggcagcaat ggcagcgaaa atattgtcgg cttccagcgg gtagtggcgg aagttgtcca
    22621 gctcgctggc gaagatatta agcagaattt ccgagtcgga agtggtgttg atgtggcggc
    22681 gtttttcttc aaacagtttt ttacgcaact cgtgagcgtt ggtcagattg ccgttgtggg
    22741 caagcgtaat gccatacggg gagttaacgt aaaacggctg cgcttcagag gcgctggagc
    22801 tgccagccgt agggtaacgc acatgaccaa tgcccatatt gccctgcaaa cgctgcatat
    22861 ggcgagcttc aaatacatcg ctcaccagcc cgttcgcttt acgcaaacgg aagcagttat
    22921 tggcatctat ggtgatgatg ccggcggcat cctgaccgcg atgctgaagc accgttaagg
    22981 catcataaat cgactggtta accggcataa caccggcgat accgacaata ccgcacatac
    23041 gtctttttcc tcgttaagcc acatctcaga gcacttacgc tctgggcaag aaacttgacg
    23101 agctttgcag ataatcaaaa aaccatctga tgataaaact gaattgcggg atcagctgtg
    23161 atttgctcca gtcttcactt ttcgacaccc cggtaaagga gtcgagaaag aagagaatgg
    23221 cggcaacaat caacacaccg cgcaacgcac cgaaacagac gcccagcacc cgatcggtgc
    23281 ctgacaaccc cgttttctcc accaactggc ctatcacgaa gttcacgata gcaccaacga
    23341 tcagggtagc gataaacagt accgcgatgg caatcccatt tcgaaccagt tcgtcttcaa
    23401 agcccgtaaa ccagactgac aggtaagtgt agtaatgact ggcaacaaag aaagcacaac
    23461 cccatgtcac cagcgataac gcttcacgaa caaagccgtg gatcaggcta accagagagg
    23521 aaaaagcaat caccgcgatt atggcgtaat caatccagac catatgtgtc ccacgatttt
    23581 acgccctgtc atcctgttcg gggcgcattc taacagaaaa agaaaacgtt tgcgtaggga
    23641 tttccttccc gcgcatcaat aaaaatggcg ctgaaaaaat attcaacgcc atcgactttt
    23701 tatgcctttg cggcatcgtg caatgcgtgt cggatgcggc gtaaacgcct tatccgacct
    23761 acggttctaa cactgcccaa gcctgataag acgcgccagc gtcgcatcag gcaagactgt
    23821 attaattcgg cgtatagccc attaccacgc cacttaagcc agaaagttgc ttcaactcac
    23881 ccagcgaacc tttcagctta tctttcgagg catccggccc aaccagaata cgggtaattt
    23941 taccctgcac tggcgtggat ggcgacgtat aaacccgata accggcaccg cgcagcttac
    24001 cgacaatctc attcacttta tcggcatttt tcagcgcacc cagttgcaca acataggctt
    24061 tacccgttgg cgctgctttt tcttccacga ccggcttcgg ttctggcttc ggcgcaggtg
    24121 gtacttcaac ctttggttta ggcggctcca ccggtttcgg cttcggtggg gcgaccggtg
    24181 caggttccgg ttcaaactcg gtgttattgg ctgcaatagt ggccggatcg agcgacggtg
    24241 ctgcggcatc acctgcccgc acctcttccg ctgcgccttc cggcggctgc gtcggcaacg
    24301 cctgggtggc ggctggcatc atatcaggct catcacgatc gcccgctttc ggcaccagcg
    24361 ggatagccgc gaactcatcc tgataatgtt ttttctgccc gtccagcagc cctggaagta
    24421 caatcacccc cagcgccacc agcacgatcg tgcccactaa ccgattctga aacttacttg
    24481 ccaccgcttc tcctcgcgtc aatcacttcc atgacatgtg cgaccgtgtg gaaagaacca
    24541 cacaccagca cggtgtcttc cgctttagcg tccgccattg cggcatccca tgcctgcgca
    24601 acgctatcaa atgatttgcc gttacccaaa tgctcaagca gttgttctgc cgtggcaccg
    24661 cgcggccctt ccagtggcgc acaataccag tcatcaacca cgcttttcaa ccaggccaga
    24721 gttccggcaa tatctttatc atgtagcata ccgataaccg ccagcacgcg cccgtttttc
    24781 ggtagcgctt tcatacgccc ggtgagatat tccgccgcat gtggattatg cgcgacatca
    24841 aaaataacgc gtggcgactc gctcacaatc tggaaacgtc ccggcaaaat tgcgctggca
    24901 atcccgtcgc gaatggcatt ttcactgact tccagcccgc tggcacgcag tgccgccagc
    24961 gccgttgcgg cattcggttg cgggacaagc ggcaacggca gattttccag cgtgccgtga
    25021 gcatcgctaa acgcccagtc atgatcggtg acggaatagt tccactcaac gccccgacgt
    25081 tgtaacagtg cacctttttc ctgcgccaca tcagcaatgg tagaaggcat ttccggctca
    25141 ccgacaattg ccggtttttc gctgcggaag atgcctgctt tctcgagacc aatactttcg
    25201 cgatctggac ccagccagtc ggtatgatcc agcgcaatac tggttactac cgcgacatcg
    25261 gcatcgacaa tattggttgc gtccagacga ccgcccagcc ctacttccag aatcaccacg
    25321 tcaagttgtg cctgcttgaa cagccacaac gccgacagcg taccgtactc gaaataggtc
    25381 agggaaatat caccgcgtgc cgattcaatc tccgcaaaag aggcggtgtg ggccgattcc
    25441 ggcaattcct ggccctgcac acgtacgcgc tcggtataac gcaccagatg aggcgaactg
    25501 tagacgccca ctttgtaccc tgccgccatc agaatcgact ccagcgtacg gcaggtggtg
    25561 cctttgccat tcgtacccgc aacggtaaac acaaatggcg ctggtttcag gacgccaaga
    25621 cgcgccgcga cctggctcac gcgctcaagg ccgagatcga tagttttact gtgcaggttt
    25681 tccagataag aaagccacga agccagaggc gacgcggctt gaggagtgcg tttgataatc
    25741 atggtatccg ctgattcgtt acggtgagaa tagcaaaagg gcagagccag tggccctgcc
    25801 cttatcagtt atcaggcctc aggttcctga tccggtaccg ggggtaccac tacgccttca
    25861 cgcggcgctt caggattcgg cgctggcaga ttcatcaact tcgccagaat gctcgccagt
    25921 ttcaggcgca tttccggacg acggacgatc atgtcgatcg cgcctttctc gatcaggaat
    25981 tcactgcgct ggaatctagg cggcagtttt tcgcgaacgg tctgttcgat aacacgcgga
    26041 ccggcaaagc cgattaacgc tttaggttca gcgatgttga gatcgcccag catggcgaaa
    26101 cttgcagaaa caccgcccat cgtcgggtcg gtcagcacgg agatgtacgg caagccgcgc
    26161 tcctgcattt ttgccagtgc cgcagaggtt ttcgccatct gcatcagcga catcagtgct
    26221 tcctgcatac gtgcgccacc agaggcggag aagcagatca gcgggcagtt atcttcctgc
    26281 gcctgctcaa cggcacgcac gaaacgtgca cccacaacag accccattga accgcccata
    26341 aaggcgaact cgaatgccgc agcgacaacc ggcattccat acagagtgcc tttcatcacc
    26401 accagcgcat ctttttcgcc ggtttctttc tgcgcagatg ccagacggtc tttatacttc
    26461 ttggagtcac gaaacttcag ctcatctttc ggctcaagct cgctacccag ctccacaagg
    26521 cttccttcat ctaacaggct atgcaggcga ttacgcgctg tcatacgcat gtgatggtca
    26581 cacttcggac agacctcaag attacgttcc agctcagcgc ggtataaaac ctgaccgcag
    26641 ctatcacact tagtccacac cccttcagga atgctcgcct tgcgggtggg agtaatgttg
    26701 cttttaattc gttcaatcca gctcattagg gacctttctg tctgaacctg gttcgatgcc
    26761 agttttatct ttggggacgc ataatgccat ttttgccccc aacagaccat gaatgttgca
    26821 cattaaaaca taacagcccg aaactttgga taaaaaagtg gtcgaaccgc ggagttactt
    26881 tttattttgc ggcgcgtgcc gcagcgcgtt tgtgacggat tatttcgatg acgcccggca
    26941 aaatagaaac cacaataatc ccgacgatca gcagcttaag gttatcctga accatcggga
    27001 ttgtaccgaa gaaatagcct gcgtaggtaa aaagcagtac ccacaacagt gcgccgatca
    27061 cgttataagc agcgaaatga cggtacgaca tgtggcccat tcccgcaaca aacggggcga
    27121 acgttctgac gatcggcaca aaacgggcga gaataatcgt tttgccgcca tgtttctcat
    27181 aaaactgatg ggttttgtcg agataactgc gacggaaaat tttcgaattg ggattactga
    27241 ataacttttc accgaacaac cgcccgatag tgtaattgac cgcgtcaccc acaatcgcgg
    27301 cgatcagcat cagcactacc atcatatgga cgttgagatc gttggtttcc agtgatgcca
    27361 acgctccggc aacaaacagc aatgaatcac ccggtaaaaa cggcattacc accagaccgg
    27421 tttcacagaa caaaattaag aacaaaatgg cataaaccca gacgccgtac tccgcgacta
    27481 gctctgccag atgcacgtca atgtgcagga taaaatcgat gaggaaataa atcaggtcca
    27541 tatttgccta tgccttgtac tcgtcatctt tcaggctgta actacgttgg ctacagcctg
    27601 aaatgttccg agcattattc tcgttagtcc gccagaaata gcgggcccat tggcggtttt
    27661 ggaagatcat accggtcagg gtaatccacc gcgaccagat acagcccttc cgcttttgcc
    27721 gttgctgccg ccagcgttct gtcctttgcc gccagcaact ctgctatcca gctctccggc
    27781 tggttgtggg caccgacttc catcaggctg ccgacaatat tcctgaccat atgatgtaca
    27841 aaggcattcg ctttgatatc aaccaccaca taaggaccgt gacgcgtgac attaatatgc
    27901 ataacgttgc gccacggggt tcgggactgg cactgcaccg cacggaacga ggtgaaatca
    27961 ttctcgccca gcaagcattg cgcagcccga tgcatccgtt cagcgtccag cggttcgtaa
    28021 aaatgggtta cccctttact cagtaccgcc gggcgcagcc gatgattgta gatgatgtag
    28081 cgataacggc gagccgtggc gctaaatcgg gcatgaaaat catcaggtac agctttaacc
    28141 caacgcacgg cgatgtcacc aggtaaattc gcatttacgc ccaaggtcca cgccgcgtct
    28201 ttgcgctgcg cggttgtttc gaaatgcacc acttgcccgg taccgtgtac ccctgcgtca
    28261 gtacgcccgg cgcagaagac ggtgatgggt tcgttcgcca cctgggagag cgccttttcc
    28321 agcttctcct gcacactgcg gacttcattc tgccgttgcc agccgtaata cttactgccg
    28381 tcgtactcaa tgcccagcgc aattttataa actggcagtt gttgctggtc ggacattagt
    28441 acagatactc ctgcaccagt ttctcggcga tttttactgc catcagcgcg ccgccaaagc
    28501 gaacgttatc ggccaccgac cagaactgga cttgctccgg cataccgtag tcattacgca
    28561 cgcagccaac agaaagatgc ggcgtacccg aagcatcccc tacctgagtc gggaattcgt
    28621 tctcttcaga gagcacaata tcttcgccct gagcaaacgc atcacgcgct tcttctgccg
    28681 ccagcggacg cagtgcttca aagttgacca tctgggcatg accgtagaat accggtgcct
    28741 ggacgacgct tgccgaaatc atcagccctt cgtcctgcag gattttgcgt acttcgtcaa
    28801 cgatacgacg ttcttcacgc acgctacctt cgctatccgg caacaacggc agcatattga
    28861 acgccagttg acgaccgaag aaatcttctt cgtcaatcgg aatgccgttg agcaatttcg
    28921 cactctgccc cgctaacgca tcgaccgctt ttttgccctg ggcggaggct gaaatcaggc
    28981 tggtaacact gatacgcgac aaaccgccct gatcgattaa cggtttcagt gccgccagca
    29041 gctggctggt cagactgtct ggtacggcga tgacattccg gttgcggtaa tcggtcagta
    29101 caaacgggtt tacttccggc accaccagcg gtacgtcggg ttcgagagca aacagtccgc
    29161 tgctgtcgat caccaggcaa cctgagttgg tcgcttcttc aacccaggca gcggtagctt
    29221 ctttgcctgc gacaaaaaat gccagctgcg cctgcgtcca gtcgaattca gcggcatcct
    29281 gcacggtgat tgttttacca ccaaagcgca gttgttcgcc tgcgctttcg ttacgtgcca
    29341 gtgcataaat ttccccaacc gggaactgac gttcagccag cgtttcaagc agggcttcgc
    29401 ccatagcgcc agttgcgccc aggacggcaa tgttccagcc ttcagacatg gtggtttact
    29461 ccagaaatag caaagctccc tgccaatgtt acagcagaga gcatgaagaa gagattaacg
    29521 tgccggatga tgaacggcgt taaaacccag tttacacagc aatgatgccg cactggcgtc
    29581 atcacaaatt acatacagag atgaccattc acggcgctca agatagtttt tgcgcagttt
    29641 atcgaactca cccggtatcc cggcgacttt acgcagcggt gcgtcatcgc ggcgcacatc
    29701 atacaccaaa tgcaccagcc ttttcagcgt cggttgatcg agcggaccat gcagcgtaat
    29761 gcggccaaac tcaggcgcgg gcagcaacgt atccagcgca acgtgttgtt catgcccaat
    29821 aaacttgcta taggcttcaa acacttgcgt cgtgccacgc gctttaccct ccagggtata
    29881 gcctgcgata tgcggcgtgc cgatatccac ttttgtcagc aattccacat taagttccgg
    29941 ctcgccttcc cagacatcca gcaccacgct taacttctgg ccttcattca ggcaggtcag
    30001 cagcgcggta ttatcgacga ctgcgccacg gcaggcgtta atcagaatcg ctccgggctt
    30061 caggctacgg atcagttttt catccgccag atgtagcgtt ttgtacggac catctttaaa
    30121 gagtggcgta tggaaagtca gaatatccgc gcgctggact aactcatcca gcgagcggaa
    30181 atcaccctca tccccacggt cggcgcgagg cggatcgcaa agtaaggttt taatccctaa
    30241 cgcttccagt cgcgcctgca atcgacgtcc aacgttacca acgcccacga tccccaccgt
    30301 acggtcgtgc agtgaaaatc catcgcgttc ggcaagcatc agcagggagg agaaaacata
    30361 ttccaccacc gcaatcgcat tacagccagg tgcagcggaa aaaccaattc ccgcctgctt
    30421 caaccatgct tcatcgacat ggtcggtccc cgcagtggca gttccaacaa atttaatggg
    30481 ttttcctgcc agcaaagatt cattcacttt cgtgaccgaa cgcaccatca gcgcatccgc
    30541 gtctgccagt tgagcgacgg ggattggacg cccgggaacc gcgatcacct cacccaaacg
    30601 gctaaataag tcgcgggcat aaggcatatt ttcatcaaca aggattttca cgtttgtgtt
    30661 acctgtatga gacgagagtt aaccggacaa gtatgccata atctcgcggc caggcatact
    30721 tgcgaagatt tcaggtataa ggatacgtaa tgatacaacc tatttccggc cctcctcctg
    30781 ggcagccacc aggtcaggga gataacctgc cgtctggcgc gggcaatcag cctttatcca
    30841 gtcagcaacg tacttcgctg gaaagcttaa tgacgaaagt gacctcactg acgcaacaac
    30901 aaagagcaga actgtgggcg ggtatcaggc acgatattgg tctgtcggga gattcaccgc
    30961 tgctttcgcg tcacttccct gccgctgagc ataatctggc gcaacgtctg ctggccgcgc
    31021 aaaaaagcca ttctgcccgc cagcttttag cgcaattagg ggagtattta cgtctgggga
    31081 atcatcgtca ggcggtcacg gattatatcc gtcataactt tggtcagacg ccgctgaatc
    31141 agctctcacc ggagcaatta aaaaccattc tcaccctgtt gcaggaaggg aagatggtta
    31201 ttccgcaacc acagcagcgc gaggcgaccg accgtccttt attaccggcg gagcacaatg
    31261 cgctaaaaca gctggtgacc aaacttgcgg cggcaacggg ggaacccagc aaacagatct
    31321 ggcaatcgat gctggaactt tccggggtga aagatggcga gttaattcca gcgaaactgt
    31381 ttaaccatct ggtgacctgg ctacaggcgc gtcagacgct aagccagcaa aatacgccga
    31441 cgctggaatc actacagatg gcgctaaaac aacctttaga tgccagtgaa ctggcggcgt
    31501 tatcggcata tatccagcaa aaatatggcc tttctgcgca atcatcgctt tcttctgccc
    31561 aggccgagga tattcttaat cagctttatc aacggcgggt taaagggatt gatccgcgtg
    31621 atatgcaacc gctgcttaat ccttttccac cgatgatgga cacgttgcaa aatatggcaa
    31681 cgcgtcccgc gctgtggata ctgttagtcg cgattatcct gatgctggtc tggctggttc
    31741 gttaaccccg acgaaatgac agtatcgtga caataatacc cagcaccgca gagatcgccc
    31801 cggcaagaaa taccgaagag taaccaaacg tggtcgccag cattcccgcc agcggcccgg
    31861 agacgccgag ggcgatatcc tgaaacgcgg cgtaaccgcc cagtgcggtg ccgcgaactt
    31921 gtgaggggac gcgtttaacc acctccacgc ccagcgcagg aaagataagc gaacatccgg
    31981 ctccggttaa cgccgcgccc gctaatgcga cccatgcacc tggggcttgc cagagcagca
    32041 acaagcccac cgtttctaca agcagagaga caatcgccac tttcacgccg ccaaaacggt
    32101 ccggcatcca gccaaacatg acgcgcatca cgacaaatgc gccgccaaac gcggtaagag
    32161 taaagcccgc catcgcccat cctttgctgg caaagtagag cgaaacgaaa gtcccgataa
    32221 ccgcaaaacc aacgccttgt agtgccagcc ctaaccctgg tttccagata agcccgacaa
    32281 cgctccacag cgatggacgt tctcccgcca gggccggtac tttgcgcact gtgccgttac
    32341 aggcccacgc cagtaagggt aataccattg tggtgatcgc cagtgcggca aaaccgtaat
    32401 ggctatgaat caacaggcca agcggcgcac caacagcgag ggcaccgtaa atcgccattc
    32461 cgttccatga catcactttg ccagagtgtt ttggccctac gatgcctaac ccccaggtca
    32521 gagcgcctgt cagtaactgg ctttcaccaa acccaagaat caaacgcccg atgaccaaca
    32581 gggcaaattt gaacggtgcg gagacaggca aaatcgccgc cagcagcaac gcgccgccag
    32641 ccagaccaca agctaacatc ccctgaagcg ccgaacgttt tgcgccatat tgatcggcca
    32701 gtcgcccggc gtaaccacgc gtcagcaccg tagccagaaa ctgaatcccg acggcaatgc
    32761 cgaccatggt attgccatag cccagatcat gatgaacaaa cagcgggata accggcaacg
    32821 gcaaccctac ggtcatgtag gtgagaaaaa ccgcaaaaga gatgcggaag agcgaaaaac
    32881 tggcagaaga tcgtgtttcg gtttggctta cagcagtcat gcattactcc agaatgcagc
    32941 gcaaggcgag gagtatcccc gtctcatctc tctggtttca gggttacggt gcgttggcag
    33001 gatttaacgc gtacgtcttt tcagaaggaa atcgacaaag cgggaagttt gcctagaact
    33061 ggtggcgatt gtcaatgatg tgaaaaaggg aaccatcagg ttcccttttg cgttagtgcc
    33121 ggaggccgca ttggcgcgta acgtcggatg cgacgctggc gcgtcttatc cgacctactc
    33181 cggcctactg cgaattaatc ttttcagctt gcgcattacc agcgtggcgt tggtgccgcc
    33241 gaagccgaag ctgttagaca taacagtggt cagttcgcga tcggtcgttt cggtcacgat
    33301 gttcagaccc gcagcctgct cgtccagctc ttcaatgttg atgctcgggg cgataaagcc
    33361 gtgttccagc atcagcagag agtagatagc ttcctgcaca cccgcagcgc ccagtgagtg
    33421 accggtcatg gctttggttg cagaaatcgc cgggctctta tcgccgaaca cttcacggat
    33481 agctgccagc tctttcacgt cgccaaccgg agtcgaagta ccgtgagagt tcaggtaatc
    33541 gattggggta tcaacgccat gcatcgccat ctgcatacaa cgtactgcgc cttcgccaga
    33601 cggagcaacc atgtctgcac catcagaggt tgcgccgtag ccaacgattt cagcatagat
    33661 gtaagcacca cgcgccagcg cgtgttccag ctcttcaacc actaccatac cgccgccgcc
    33721 agcgataacg aaaccgtcac ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc
    33781 gttgtattta gtagatagcg cacccatcgc gtcgaattcg caggccattt cccagcacag
    33841 ctcttcgccg ccgccagcaa acacgatgtc ctgtttgccc agttggatct gctctactgc
    33901 gttaccgata cagtgtgcgg aagtcgcaca cgcggagctg atggagtagt taacgccatg
    33961 aattttaaac ggcgtggcga gacaggcaga aacgccggat gccatcgctt tagtgaccac
    34021 atacgggcca accgctttca ggccgcgcgg gccgcgcatt gcgtcagcgc cgaacacctg
    34081 gaaacgcggg gaaccgccgc cggaacctgc aatcaggcca acgcgcgggt tattctggta
    34141 agcttccgga gagaggcccg catcagcgat tgcctgctcc atagaaagga atgcataaat
    34201 ggatgcgtcg ctcataaagc gcacaacttt gcggtcaatg aggccagtgg tatccagttt
    34261 tatgttgccc cagacgtggc tacgcatgcc ggaatccttc agctcctgag agaaagtgat
    34321 ccctgaacgt ccttcacgca gagatgccag gacttcctgc tggttattac cgatgctgga
    34381 aacaatgccc aggccagtaa tcactgcacg tttcattcaa tacctctgta agtcgcacat
    34441 agagtaagtt tcgaatgcac aatagcgtac acttgtacgc cgaacaagtc cgatcagcca
    34501 tttaatagag atatttgcgc agccttacac acatcgctaa gatcgagcca ccgcctgtaa
    34561 gacgagtaac ttacgtgaaa cactactcca tacaacctgc caacctcgaa tttaatgctg
    34621 agggtacacc tgtttcccga gattttgacg atgtctattt ttccaacgat aacgggctgg
    34681 aagagacgcg ttatgttttt ctgggaggta accaattaga ggtacgcttt cctgagcatc
    34741 cacatcctct gtttgtgata gcagagagcg gcttcggcac cggattaaac ttcctgacgc
    34801 tatggaaggc atttgatcag tttcgcgaag cgcatccgca agcgcaatta caacgcttac
    34861 atttcattag ttttgagaaa tttcccctca cccgtgcgga tttagcctta gcgcatcaac
    34921 actggccgga actggctccg tgggcagaac aacttcaggc gcagtggcca atgcccttgc
    34981 ccggttgcca tcgtttattg ctcgatgaaa gccgcgtgac gctggattta tggtttggcg
    35041 atattaacga actgaccagc caactggacg attcgctaaa tcaaaaagta gatgcctggt
    35101 ttctggacgg ctttgcgcca gctaaaaacc cggatatgtg gacgcaaaat ctgtttaacg
    35161 ccatggcaag gttggcgcgt ccaggcggca cgctggcgac atttacgtct gccggttttg
    35221 tccgccgcgg tttgcaggaa gccggattca cgatgcaaaa acgtaagggc tttgggcgca
    35281 aacgggaaat gctttgtggg gtgatggaac agacattacc actcccctgc tccacgccgt
    35341 ggtttaatcg tacgggcagc agcaaacggg aagtagcgat tatcggcggt ggtattgcca
    35401 gcgcgttgtt gtcgctggcg ttattacggc gcggctggca ggtagcgctt tattgcgcgg
    35461 atgaagcccc cgcgctgggt gcttccggca atcgccaggg ggcgctgtat ccgttattaa
    35521 gcaaacacga tgaggcgcta aaccgctttt tctctaatgc gtttactttt gctcgtcggt
    35581 tttacgacca attacccgtt aaatttgatc atgactggtg cggcgtcacg cagttaggct
    35641 gggatgagaa aagccagcat aaaatcgcac agatgttgtc aatggattta cccgcagaac
    35701 tggctgtagc cgttgaggca aatgcggttg aacaaattac gggcgttgcg acaaattgca
    35761 gcggcattac ttatccgcaa ggtggttggc tgtgcccagc agaactgacc cgtaatgtgc
    35821 tggaactggc gcaacagcag ggtttgcaga tttattatca atatcagtta cagaatttat
    35881 cccgtaagga tgactgttgg ttgttgaatt ttgcaggaga tcagcaagca acacacagcg
    35941 tagtggtact ggcgaacggg catcaaatca gccgtttcag ccaaacgtcg actctcccgg
    36001 tgtattcggt tgccgggcag gtcagccata ttccgacaac gccggaactg gcagagctga
    36061 agcaggtgct gtgctatgac ggttatctca cgccacaaaa tccggcgaat caacatcatt
    36121 gtattggtgc cagttatcat cgcggcagcg aagatacggc gtacagtgag gacgatcagc
    36181 agcagaatcg ccagcggttg attgattgtt tcccgcaggc acagtgggca aaagaggttg
    36241 atgtcagtga taaagaggcg cgctgcggtg tgcgttgtgc cacccgcgat catctgccaa
    36301 tggtaggcaa tgttcccgat tatgaggcaa cactcgtgga atatgcgtcg ttggcggagc
    36361 aaaaagatga ggcggtaagc gcgccggttt ttgacgatct ctttatgttt gcggctttag
    36421 gttctcgcgg tttgtgttct gccccgctgt gtgccgagat tctggcggcg cagatgagcg
    36481 acgaaccgat tccgatggat gccagtacgc tggcggcgtt aaacccgaat cggttatggg
    36541 tgcggaaatt gttgaagggt aaagcggtta aggcggggta atctgctctc gcagtgtttg
    36601 ccggatgcgg cgtgaacgcc ttatccaacc tacgtgtgac gtgtaggcct gataagacgc
    36661 gataagcgtc gcatcaggca tggtgctcca gacgccgcaa cttacagctg tgacgcctgc
    36721 tgaaacaggt tttcccacat atcggtcacc agcgcctggt cacgcggcga caattcaccg
    36781 gcaccaatgg ctttttccag actctggcta acggtcgtat gcaccgcctg agcggagtgg
    36841 tcatcaccac tttccagttc tgcgatggct aatgtcaggt ggccacgcaa atacccactg
    36901 gcaaacaact catcatcact ggcatggtca accatgccgt cgattaatgc cagaatgcgt
    36961 gattcaaact ccgcgatcat cttctttcct cattgtaaaa cgtggcgctg gcttcagttg
    37021 agcgcttccg gccacgggaa ctgttccgcc gtaagttccg gcgtgtgata ataattttgt
    37081 agtgctttaa tgaagcgtgc cgggcgttcc ggaatgccat ttgtcagata atccatcacc
    37141 tgcgcatgta cccgccgttg aaacaccaca cgatccggtt cgaaatcccc ttccagattg
    37201 tcgcaactga cgttaaacgg atatccagcc gccacgcaga ataaccagtc gaacgcctgc
    37261 ggcttcactt caacatcttc aaactggctt tgcgtctggg catcgcgtcc gtccgggcaa
    37321 taccagtagc cgaaatcaac cagctcacgg cgcgcttttc cggcaataca ccagtgcgaa
    37381 atctcatgaa ttgcgctggc ataaaagcca tgagcaaaga cgattcggtt atacggtact
    37441 tccgcatcag caggaagata gatcggttcg tcgtcgcctt taatcagacg ggtattaaat
    37501 tcatcggcaa agcagctatt aaaaatttca atcaactgct cgtagtggtg tgtactgttc
    37561 attagttcat ccccaaccag tggaggatct cctgtccgtg gctatcgtaa agtaatttgg
    37621 cactcatcac cgccgagacg ataacaatca tcgggcggat cagcttttgt cctttgctca
    37681 acaccaaccg tgaacccatg cgcgcgccga ggaactgccc caccagcatg acaaagcccg
    37741 tcgcccaaat cactttgccg ccgagaataa acagcagcag accgccaatg tttgacgttg
    37801 cgttgagtaa tttggcatga gccgtggctt tggcgaggtt gaatccgcac agcataacga
    37861 aggccagcgc gtaaaacgat ccggcagccg ggccaaagaa tccgtcataa aaaccgacgc
    37921 agccaccggc aattaacgcg aagggtaaac cgtacatccg gcgctggcga tcttcttcac
    37981 ccagctttgg catcagcaag aaatagaggc cgatacaaat caccagaatg ggcaaaatct
    38041 gccgtaagac atcagcctga acgtactgta ccaacagtgc gccgctcatt gagccgacaa
    38101 aggtcatggc aatattgagt ttctgatcgc ttaaactaac cactttgcgg cgaataaagt
    38161 agatagtggc ggaaatagag ccgccgcagg cttgcagttt attggttgcc agcgcgttag
    38221 cgggagacat tcccgcagcc atcaatgccg gaatggtgag taacccaccg ccaccggcaa
    38281 tcgagtcgat aaatcccgcc agcatggcga caaaaaagag aactcccagc aacagcggag
    38341 aaaccataaa caggctatta aacgtttcca ttagatcacg tgctcatcca gtagcgcctg
    38401 gcaggaaggc ggcaacggag gcggtgtctt cttctcaggc tttgttgttc ccggttttgg
    38461 aggttcaaac cagctttgca gttctgcccc gcaaccatcg cctgatggcg gtaaaggttg
    38521 atcttcacac tccagactat cggcagggca acgtaatcgt acatgcatat gcgcgcgatg
    38581 ctggaaccag ggtcgcactt tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag
    38641 ttgttgttta atcgccggat taacaaaaat gcgcgtgacg tctttgtcct gggcggcgag
    38701 tttgatcaag ctgaaaattt ctggcttcca cagcgtggag acaacgtgtt taccgtcgcg
    38761 ggaaactaag tccagtgctt gcgggcgcaa gagctgcgcg gaggtccagc gagttttcgg
    38821 cagttgcaga aagatatcga catccagtcc ggtctggtgg ctggcatgac cgccgttgaa
    38881 acgcccacca gcgggcatcc ccatatcgcc aatcagcacc gtacccatgc ccagattgct
    38941 cacctggcta ctcagacgct ggataaacat caccagatcc gggtgaccga aatagcgacg
    39001 ctgatcggta cgcatgacct gataatgttc ggactgtatc ggcaacgtgt cagcgccgac
    39061 aatacagcca ttagaaaaac tgcctatcga ttgtgcgcta cccggcacag gttgggttat
    39121 tttttgccac ggcgtcgctg ccaggctagc actactggca agcagagcca gcagcgcaat
    39181 cgcggttttg ttcatttttt accagcgtgg aatatcagtc ttcacatcgg cattttgcgc
    39241 ccgctgccgt aacaggtgat ccattaaaac gatcgccagc atcgcttcag cgatcggcac
    39301 tgcacggatc ccgacacagg gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc
    39361 aaagcggtta atagtgcgcc ccggcacggt aatgctggag gtcggtttca gcgccatatg
    39421 agcaatgatt tgctgcccgc tgctgatacc gccgaaaatg ccgcccgcat ggttgctctg
    39481 gaaaccgtct ttggtgattt cgtcgcggtt ctggctgccg cgcagcgcca ctacgtcaaa
    39541 accgtcgcca atttccacgc ctttcaccgc gttgatgctc atcagcgcat gggcgatgtc
    39601 ggcatccagg cggtcaaaga ccggctcgcc aagtccggcg gggacaccac tggcaacaac
    39661 ggtgactttc gcgccgatgg agtcgccctc ttttttcagc gcgcgcatca gttcatctaa
    39721 cgcgtcgatt ttgtcggggt ccgggcagaa gaacggattt tgttcaacca gcgaccagtc
    39781 tttgatttcc agcggaatgt cgcccatctg ggtcaggcag ccgcggattt caataccaaa
    39841 tttctcggcg agatattttt tggcaatcgc ccctgccgcc acgcgcatgg cggtttcgcg
    39901 ggcggaagaa cgtccaccgc cgcgataatc gcgcagaccg tatttttgtt cgtaggtgta
    39961 atcagcatgg cctggacgga agacgtcttt aattgcgctg taatcctgag aacgctggtc
    40021 ggtattttcg atcaacaagc caatgctggt gccagtagta acgccttcaa aaacaccgga
    40081 gagtattttg acctgatccg gctcgcggcg ctgggtggta tagcgcgatg tcccagggcg
    40141 acgacgatca aggtcatgtt gcaggtccgc ttccgtcagc ggaatgcctg gcggaacacc
    40201 atcgacgatg cagccgagcg ccagcccgtg cgattcgccg aaggtggtta cgcgaaagag
    40261 ttgtccaatt gtgtttccag ccatcacggc tccgttattg ttgtgtttgc gtgtttactt
    40321 aatctttata aatcgcgaaa tgttctcgtg cggcaataag ctgctcttta gtgagcataa
    40381 acacaccatc gccgccgtta tcaaactcca gccaggtgaa cggaacatcg ggatattgtt
    40441 ccataagatg taccatgctg ttgcccactt cacaaatcaa cacgccatca tcagcaaggt
    40501 aatctgccgc gttaccgaga atgcgacgcg tcagtttcag gccgtcggtg ccagatgcca
    40561 ggcccagttc cggctcgtga cggtattcgt ttggcaggtc ggacatatct tccgcgtcga
    40621 catacggcgg gttagtgaca atcagatcgt attgcacttt cggcaaatca cggaacagat
    40681 cggaacgaat cggaatgacg ttgtggatca gaccgtgttc ttcgatgttc tgttcggcaa
    40741 ccgccagcgc gtcgggagag atatctaccg cgtcgacttc cgcttccggg aaggcataag
    40801 cacaggcaat ggcgatgcag ccgctaccgg tacacatatc taaaatatgc tgcggttgct
    40861 tgctgatgag tccggcaaat ttattgttga tcagttcacc aatcggcgag cgcggcacca
    40921 gcacgcgttc atcgacgtaa aattcatgac cgcagaacca ggctttgttg gtcaggtaag
    40981 ccaccggaat gcgctcgttg acgcggcgga tcacgcgttc aacaatacgg tgtttttcgc
    41041 tggaggtcag acgcgcggtg cgcatatctt ccgggatatc cagcggcagg tagagcgatg
    41101 gtaacaccag ctgtacggct tcatcccacg ggttatcggt gccgtgaccg taccagatat
    41161 ttgccgcgct gaagcggctt accgaccagc gcaatatgtc ctgaatggtt tgcagctcat
    41221 ttactgcttc atcaacgaaa attttatcca cgtattcctc cagggcatga tcgcaataat
    41281 ttcggcggct agtttgccat gaagatgacg ataaatcagc attcacgcgc ggtgagtgag
    41341 gaaaaatacg tttaaaacga tcgattgtgc tacgagtcgg gtaaactgta ggaaaattag
    41401 aaatagagac agataaatga aaaagaaaac aacacttagc gaggaggacc aggctctgtt
    41461 tcgccagttg atggcgggga ctcgcaagat taagcaggac acgattgtcc accgaccgca
    41521 gcgtaaaaaa atcagcgaag tgccggtgaa acgcttgatc caggagcagg ctgatgccag
    41581 ccattatttc tccgatgagt ttcagccgtt attaaatacc gaaggtccgg tgaaatatgt
    41641 tcgcccggat gtcagccatt ttgaggcgaa gaaactgcgc cgcggcgatt attcaccgga
    41701 attgtttttg gatttacacg gtctgacgca gctgcaggcc aagcaggaac tgggcgcgtt
    41761 gattgccgcc tgtcgccgcg aacatgtgtt ttgtgcctgc gtgatgcatg ggcacgggaa
    41821 gcatattttg aagcaacaaa caccgctgtg gctggcacaa catccgcatg tgatggcatt
    41881 tcaccaggca ccgaaagagt acggcggtga tgcggctttg ttggtgttga ttgaagtgga
    41941 agagtggttg ccgccggagt tgccctgaat gctggcagca aatgccggag cagcgtaaac
    42001 gccttatccg gcctacaaat atgtgataat tcaataaatt gcaattcact tcgtaggcct
    42061 gataagacgc atcagagcaa tactacgaac ttcagttcat tcaatacata ctgaaaacga
    42121 cacgtcgaat caatcggttg cccactactg gtattcacat tcaccacaat atcgtgtagt
    42181 actttatccg ttaccggatg acgagttggc gtaaccgtta ccgttgattt atcacaaccg
    42241 taagaggtaa aggttaacaa ttctgatacc gcgcggcagt atgctcctct gccatcatta
    42301 tcagaaaaat tactgccatc acagagtctg ccgccttcca tcaggtaggt cgaaagcgtg
    42361 gtggtcactg ttgaccccgt ggactgaatt cgtaacgcct tattatggct gacataattt
    42421 tcactccagg agatatcgta tgaagcgacc ggtatcgttg ggcatattgt gttatgcact
    42481 gtaggggccg tatagcttcc aaatccgggt aaatctgtat aggaataaaa ggatgcgatc
    42541 caagggcaac cataatcatc agatgcaacg gtaattttaa acgtcatata tgcacaacga
    42601 aaaattttcg tatctatgcc agggcaattt gagttattaa ttgtatcaaa gtcagtcagt
    42661 accaaagttg gagctgaacc cgacccagac caggaaataa cccgcaacgt atcgccttta
    42721 tacgcaaaag aatcatattt cccatatgca acctcctttg gcgttatcac cgtaacattg
    42781 aaaacactgc tcgctacagc accggttacc ttgtccatgg cataataaac tctcacactg
    42841 tcaaaactcg cactcataag tttatctcca gcataactct tagatgcgcc gacaacactc
    42901 agaacgagca gcattaggct cgcgaaaatt atggtccatt ttttcatttc agaaatactc
    42961 aaaggttgcc gtcactgtgg cactaaagtc tccggacgtt acatctcgcc cgctctttgc
    43021 ctgtaaccag gtattaaaat ttaggctgtt atttccctga ttcagcggga gcgtgacgcc
    43081 tgttgtattg ttgattggca ccgatgtgcc tgccgcagtt tcaataccaa tcccagcgcc
    43141 ggatgctgat gacgaggagt caaaggccag aaagccgggt agagcactat cttctgttcc
    43201 tgtcaacgtt actttgacat taaacaatgt agaactatga caatctttca attgaaacac
    43261 caatggcact cgctctgact gcccaaggtt cattaaatca ctggcggcaa tggcaggaaa
    43321 atgtacttct gccaactggc taccattaat cactggcgta cacgatttac tgattaaatt
    43381 gccggtaaag tgcaaattat ctcctgctat tgtggttcca ctgcccagca acatcaggct
    43441 actaataaaa aacaacgtac gctttttctt cattggtaat ctacctgtaa cgtggcccag
    43501 gcctcaaagt ccgcctctgg cagaatgaca ccactctttt ttactggcac agccgttaac
    43561 accggtaaat cggtttcatt caccacaagt ggcgtattga tcgtaaaact ctgccctgcc
    43621 tgtttgagct gaatgcctaa accagtgata ttgctgctta ctgcagcatt atcaaaatcg
    43681 cttggtgtgc cactccagct caaggtcatt tccattaatg ggtcgcgaac cgccgtatcg
    43741 caggtaatgg aatacggcac ggtttgctca taattgctgc cattaatgtc atcaataagc
    43801 acatcctgaa aagttatttc aatggtttga gcgttgctaa tcgtgcagtt aggcggctgc
    43861 actaaataac cgtgaaaaga gacgtttgag agtgccatcg agcaaaagcc ccacagcaaa
    43921 ataagcgaga tccgtttcat tggtaatcca ccacaattgt ggcactggcg ttaaattcag
    43981 ctgccgctaa cgttgtacca ctctgtttca ccggcacagc ttccagaacg ggaactcccg
    44041 aggagaaatt aaatggcaac catgagccag aggttaagtc gagtattgtg tgatcactac
    44101 ttttctgaac ccgaatacca aacccaggaa tacccgtgct gataactgtt tcaccgttaa
    44161 tcacgaccgt tgtcgcctgc atctgtagac gcaggtcatc cattgccaga ttgttacaaa
    44221 caagcgagta gtcaatggga cgtttataat caaccctatt gatcttatta atgaatacat
    44281 taccaaactc aactgccgca ccatttaccg tacaaggagg aggggcatcg acaagcaccc
    44341 gtaaattcat acttactgac ttactcgctg caactgcaaa gccgctacca acacaacata
    44401 tcagtaaccc agttaccgcc cagacagaac aagatccttt catactggct ctcccggcct
    44461 cagcctttgc ggctctgctc ttcatcgacc tagcaactgt taccgttgca agtgaaaaag
    44521 agcggcatcc gagcgccata gtcgttgaca taggtcagta ccggcacgct acccatcttc
    44581 acattcagcg gtgcagaggt tttcggcgac atcactattg gcgagaaacc ggctgccgga
    44641 ttgccatttt tctgtgtgct ggcattactg aaaatgacgt aatacgacgt cgggttattg
    44701 acggtaaatt cctgaccgtt gcgggttaaa gtaatcttat attgctaggg gtttttcatg
    44761 ctgacgttct ccagcgcctt cggtcgccag aacaacttaa tacgcgtttg tagtgctatt
    44821 tgcagcgtgt tggctttgtt tgattttggc gggatctcac gcacgttgaa gtagaacagg
    44881 ctttcacgat cggcaggcaa tttgttgata tccggcattc cctgcacttt tacctgcccg
    44941 ttcatcattg aatcgatgcg ttgcaccggt ggcagaacag ttaacggtga actgatcttg
    45001 ttaccctttt catcttcgat ccagctttgg gccagataag gcagtttcgg atcgttattg
    45061 cgcaacgtca cgctgattga tttgtcgctt tcattaaata tcaaacgggt acgatctggc
    45121 gttacagaag cctgggcaga caaactggca gcgctaagca gtaacgctag cgtcgtagca
    45181 cccaatttgg cagagcaaag taaatcagac atggatcatt ccttaatcac gttattggtt
    45241 agctgaaaca gaaacagggg gattggtggg tgtcacctgt tgcgtctgct cctgaatgac
    45301 aggctttatg tcatcaggaa caaccggcgc gacgttgcct ttatgctggc atggcagtaa
    45361 caagccgtta aacagatcgg caggcagcgg gtccggcagg ttgatatcgc aatgcgcaac
    45421 accactccag aacacactca tgtgttcgcc aggtttcact ccagccagat aaacattgcc
    45481 gtcatcatcg acaaggccca ctgtctgctc gttatcattt tttacttctg cgccaaacgg
    45541 aggatggctg ccgtcttgta aacgcagcac cgccatcgct ttttgaccac tgatgacggc
    45601 aaatttgcgg tagccaatcg ccccttcagt tagcgtggct tgtaccaccg actgggttgc
    45661 ttcggcgttt tccggcagtt tgttgaggtc gatatacgcc tgattgcggt aatagttatt
    45721 gacgtcagaa acgacggctt taccaaacat attggtataa acagcagcac cgttaccttc
    45781 taccggaaca tcggcaacgc catcggcatc aatcaacaag cgtgtcccgc ccatattctg
    45841 ggtacggtga agtgcgccac cgtgggcagt aagcgttgcg ccgccctgta acgacaagcc
    45901 cgcagaggtg tattgccctt catggtagtt cgcactgagg tcaacctgcg ccagcgaacc
    45961 atcatggctg taatagccgt caacgctggt gtgtttgtca ctggtgccga cgttcaactg
    46021 atagtgagtc gcgtcatcga cacggctgaa ataaccgacc tgactgctgt ccgtcccact
    46081 gccatagtac cgttatagct aacggtactg ttgtcgcccc acggcatact gagcgaaatg
    46141 tacatgcctt tgtcggcctg gttgtcatac tcgtaacggt agccagtcat cgagatgctg
    46201 acattacgaa tactgcccat attgaaatag tgcgagagca tgatgttgta gtttgtctgc
    46261 tcctcgcgat cccagtaggt atggcgggta tagttgagat aaaccgaaac acccgcatcg
    46321 cggaagttct ggttataagt ggcggtgtac atctctttgt cgttgcccgt gcgtaccatt
    46381 ccgctgtcac tggcatccag atactcgctc atggtcataa agttctcttc cgagaagcga
    46441 tatccagcaa aagtaacgcg gctgttgagc tggtcaaagt ctttggaata actcacacgg
    46501 aaggagttac cgtccagcga acctttgcca taagcggtgt ctttatccag tttggtgtgc
    46561 gagtgagtaa catcaaacgc gaccgcgccg aatgtagaca aatcgcgacc gacaccaagc
    46621 gccgcagact gatagttttc atctcccagt gcgccgccat ataacgacca gccgttagcg
    46681 atcccccagg aagcttcagc accagaaaaa aattcaccct cgacatggtg tccccactct
    46741 tgcggacggc ccatcatgat cttatagcga acctgacctg gacgagtgag gtatggcatc
    46801 gaggcggtgc tgatgtcata ttcctgcacc tggccgttct gttcttcaat gcgaatatgc
    46861 aacgtaccgg agacagaatc accaagatcc tgaatacgaa acggtccagc cggaacctgc
    46921 gtttcgtaaa tcacacgccc catctggctg acggtcactt ttgctgtggt gtgtgccacg
    46981 ccggaaatgt ctggcgcgta gccgcgcaga ttgggaggca acatttgatc gtcagtactg
    47041 acactgccac caacatagtt aaaaccatca aaaatatcgg atctgaggta atcctcgccc
    47101 agcgccagtt tggcttttaa tgatggtaac gcccgcctgg catagtagcg actccactcc
    47161 catttttttg agtttcatcg ccgctgaatt catcgtcatc attactgcga gtatgttgat
    47221 agttagtctg ccagtcagca cgcatacgcc acggccctag gttaacccca accgtcccgt
    47281 tgccgctgat ctcgttacta tcatcaccgc cattttcttc gtgccgggtt tgtgcgttga
    47341 tgctgtagtc cgcgacgatc ccggagatgc cgtcatccca acgtgaaggc ggatccctgt
    47401 cgggataagt atattcgagg taagcctgtg gcagtgaaat gactaatgcg gactggctta
    47461 aatcagcctt aatttccatg ccttccagtt gaccggattt caggcattta gcatcgtggc
    47521 tccattgcag atttttcgcc acgtcttctt ttaaaccaaa ctgcgctacc agttccggtg
    47581 tcagacaagc atagcttttg ctcgcgtcat cttcaccagc ataccagtaa atatcgtact
    47641 cttccgccaa tggctgttta tttagttgaa cctgtaaatt gtattttccg ggctcaacat
    47701 atccctggct ggaaaaacgc ttcatatcaa tttttgtgtc gccttttaat tccagaaaac
    47761 gggaatcaaa ctgaatgtcg tcttcagccc agacactgct ataactccct gacattgcca
    47821 gcgcaatgca ccacggaaga atccgtaatc gaaaaagaga atggtcaggc atacacgttt
    47881 ctatccgttt ttattcttgt tcggtcaaaa tacatttaaa tcccccatag cagtgtcgct
    47941 atgggggtat gccttacagg taagtaatct ggaaggtggt atttgcttca aaattaccca
    48001 gatctggcgc atcagctgcg ccgaccagcc aggcgttaaa gttcagtgtc tgcttcgctt
    48061 tgcctttgtt ggtagaagtg tctttcacta ttttctggtc aatacccatg ccgcttttgt
    48121 aagaggtacc cagagagtca ccgatcgcca ggctaacatt gttaaatgcc gcaccggtat
    48181 cggtattgaa aatggtgtaa taattgccgc tatttgcaga agaaacggta ccagtaaagg
    48241 tagtggtcat tgtttcctga gtatcaaata cgcaatcttg cagacgaatc tggaaacttt
    48301 tcggcgtggt ggtgccgtta tttttcagtt tttcggcacc gatatcaccc atatcaactt
    48361 ccagtttgtg atcgtccgga acgatagaac acactgagtc ttcaatggtg ccgtaaaaac
    48421 gtgcagtacc gttgttaccc gcagcgattg ttgccgtaga ggtgaacacg cccatcacca
    48481 ttgccgcagc aata
//
LOCUS       DAZPEU010000010        48021 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPEU010000010 DAZPEU010000000
VERSION     DAZPEU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.010"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(93..3242)
                     /gene="acrB"
                     /locus_tag="ACHU2L_000488"
     CDS             complement(93..3242)
                     /gene="acrB"
                     /locus_tag="ACHU2L_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR1355485.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3265..4458)
                     /gene="acrA"
                     /locus_tag="ACHU2L_000489"
     CDS             complement(3265..4458)
                     /gene="acrA"
                     /locus_tag="ACHU2L_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR1355486.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4600..5247
                     /gene="acrR"
                     /locus_tag="ACHU2L_000490"
     CDS             4600..5247
                     /gene="acrR"
                     /locus_tag="ACHU2L_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR1355487.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5375..8737
                     /gene="mscK"
                     /locus_tag="ACHU2L_000491"
     CDS             5375..8737
                     /gene="mscK"
                     /locus_tag="ACHU2L_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR1355488.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8949..9110)
                     /gene="rsmS"
                     /locus_tag="ACHU2L_000492"
     CDS             complement(8949..9110)
                     /gene="rsmS"
                     /locus_tag="ACHU2L_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR1355489.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(9124..9651)
                     /gene="priC"
                     /locus_tag="ACHU2L_000493"
     CDS             complement(9124..9651)
                     /gene="priC"
                     /locus_tag="ACHU2L_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR1355490.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9721..10098
                     /locus_tag="ACHU2L_000494"
     CDS             9721..10098
                     /locus_tag="ACHU2L_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR1355491.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10251..10802
                     /gene="apt"
                     /locus_tag="ACHU2L_000495"
     CDS             10251..10802
                     /gene="apt"
                     /locus_tag="ACHU2L_000495"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR1355492.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10931..12856
                     /gene="dnaX"
                     /locus_tag="ACHU2L_000496"
     CDS             10931..12856
                     /gene="dnaX"
                     /locus_tag="ACHU2L_000496"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR1355493.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12189..12253
                     /gene="dnaX"
                     /locus_tag="ACHU2L_000496"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12909..13238
                     /locus_tag="ACHU2L_000497"
     CDS             12909..13238
                     /locus_tag="ACHU2L_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR1355494.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13238..13843
                     /gene="recR"
                     /locus_tag="ACHU2L_000498"
     CDS             13238..13843
                     /gene="recR"
                     /locus_tag="ACHU2L_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR1355495.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13953..15827
                     /gene="htpG"
                     /locus_tag="ACHU2L_000499"
     CDS             13953..15827
                     /gene="htpG"
                     /locus_tag="ACHU2L_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR1355496.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16008..16652
                     /gene="adk"
                     /locus_tag="ACHU2L_000500"
     CDS             16008..16652
                     /gene="adk"
                     /locus_tag="ACHU2L_000500"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR1355497.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            16784..17746
                     /gene="hemH"
                     /locus_tag="ACHU2L_000501"
     CDS             16784..17746
                     /gene="hemH"
                     /locus_tag="ACHU2L_000501"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR1355498.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17743..18702)
                     /gene="aes"
                     /locus_tag="ACHU2L_000502"
                     /pseudo
     CDS             complement(17743..18702)
                     /gene="aes"
                     /locus_tag="ACHU2L_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            18854..20158
                     /gene="gsk"
                     /locus_tag="ACHU2L_000503"
     CDS             18854..20158
                     /gene="gsk"
                     /locus_tag="ACHU2L_000503"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR1355499.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(20291..21967)
                     /gene="ybaL"
                     /locus_tag="ACHU2L_000504"
     CDS             complement(20291..21967)
                     /gene="ybaL"
                     /locus_tag="ACHU2L_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR1355500.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22205..23425)
                     /gene="fsr"
                     /locus_tag="ACHU2L_000505"
     CDS             complement(22205..23425)
                     /gene="fsr"
                     /locus_tag="ACHU2L_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1355501.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23643..25295
                     /gene="ushA"
                     /locus_tag="ACHU2L_000506"
     CDS             23643..25295
                     /gene="ushA"
                     /locus_tag="ACHU2L_000506"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR1355502.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25332..25811)
                     /gene="ybaK"
                     /locus_tag="ACHU2L_000507"
     CDS             complement(25332..25811)
                     /gene="ybaK"
                     /locus_tag="ACHU2L_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR1355503.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26015..26809)
                     /locus_tag="ACHU2L_000508"
     CDS             complement(26015..26809)
                     /locus_tag="ACHU2L_000508"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR1355504.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26947..27288
                     /locus_tag="ACHU2L_000509"
     CDS             26947..27288
                     /locus_tag="ACHU2L_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1355505.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27503..30007)
                     /gene="copA"
                     /locus_tag="ACHU2L_000510"
     CDS             complement(27503..30007)
                     /gene="copA"
                     /locus_tag="ACHU2L_000510"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR1355506.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30269..31201
                     /gene="glsA"
                     /locus_tag="ACHU2L_000511"
     CDS             30269..31201
                     /gene="glsA"
                     /locus_tag="ACHU2L_000511"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR1355507.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31204..32496
                     /locus_tag="ACHU2L_000512"
     CDS             31204..32496
                     /locus_tag="ACHU2L_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1355508.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            32527..32624
                     /locus_tag="ACHU2L_000513"
                     /pseudo
     CDS             32527..32624
                     /locus_tag="ACHU2L_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32621..33028
                     /gene="cueR"
                     /locus_tag="ACHU2L_000514"
     CDS             32621..33028
                     /gene="cueR"
                     /locus_tag="ACHU2L_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR1355509.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33029..33487)
                     /locus_tag="ACHU2L_000515"
     CDS             complement(33029..33487)
                     /locus_tag="ACHU2L_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1355510.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33484..34401)
                     /locus_tag="ACHU2L_000516"
     CDS             complement(33484..34401)
                     /locus_tag="ACHU2L_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1355511.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34547..35224
                     /gene="fetA"
                     /locus_tag="ACHU2L_000517"
     CDS             34547..35224
                     /gene="fetA"
                     /locus_tag="ACHU2L_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR1355512.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35211..35990
                     /gene="fetB"
                     /locus_tag="ACHU2L_000518"
     CDS             35211..35990
                     /gene="fetB"
                     /locus_tag="ACHU2L_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR1355513.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36053..36907)
                     /gene="cnoX"
                     /locus_tag="ACHU2L_000519"
     CDS             complement(36053..36907)
                     /gene="cnoX"
                     /locus_tag="ACHU2L_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR1355514.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(36968..37777)
                     /gene="ybbO"
                     /locus_tag="ACHU2L_000520"
     CDS             complement(36968..37777)
                     /gene="ybbO"
                     /locus_tag="ACHU2L_000520"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1355515.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(37767..38389)
                     /gene="tesA"
                     /locus_tag="ACHU2L_000521"
                     /pseudo
     CDS             complement(37767..38389)
                     /gene="tesA"
                     /locus_tag="ACHU2L_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            38360..39046
                     /gene="ybbA"
                     /locus_tag="ACHU2L_000522"
     CDS             38360..39046
                     /gene="ybbA"
                     /locus_tag="ACHU2L_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR1355516.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39043..41458
                     /gene="ybbP"
                     /locus_tag="ACHU2L_000523"
                     /pseudo
     CDS             39043..41458
                     /gene="ybbP"
                     /locus_tag="ACHU2L_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(41676..41831)
                     /gene="dinQ"
                     /locus_tag="ACHU2L_000524"
     CDS             complement(41676..41831)
                     /gene="dinQ"
                     /locus_tag="ACHU2L_000524"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1355517.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(42171..43265)
                     /gene="mnmH"
                     /locus_tag="ACHU2L_000525"
     CDS             complement(42171..43265)
                     /gene="mnmH"
                     /locus_tag="ACHU2L_000525"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR1355518.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(43334..44260)
                     /gene="allS"
                     /locus_tag="ACHU2L_000526"
     CDS             complement(43334..44260)
                     /gene="allS"
                     /locus_tag="ACHU2L_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR1355519.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            44490..44972
                     /gene="allA"
                     /locus_tag="ACHU2L_000527"
     CDS             44490..44972
                     /gene="allA"
                     /locus_tag="ACHU2L_000527"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR1355520.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            45050..45865
                     /gene="allR"
                     /locus_tag="ACHU2L_000528"
     CDS             45050..45865
                     /gene="allR"
                     /locus_tag="ACHU2L_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR1355521.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            45955..47736
                     /gene="gcl"
                     /locus_tag="ACHU2L_000529"
     CDS             45955..47736
                     /gene="gcl"
                     /locus_tag="ACHU2L_000529"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR1355522.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            47749..>48021
                     /locus_tag="ACHU2L_000530"
     CDS             47749..>48021
                     /locus_tag="ACHU2L_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR1355523.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        11423 a        12501 c        12785 g        11312 t
ORIGIN      
        1 gagatcctga gttggtggtt caattactcc ttaatgttcg taggttatgc ataaaaaggc
       61 cgcttacgcg gccttagtga ttacatgttg tatcaatgat gatcgacagt atggctgtgc
      121 tcgatatctt cattcttgcg gctaaagcgg cggcgaacca ccacaaagaa taccggaacg
      181 aagaagattg ccagtaccgt tgcggtcacc atcccgccca ttacaccggt acctactgcg
      241 ttctgcgcgc cggaaccagc accagtactg ataaccagcg gcataacgcc gaggataaac
      301 gccagcgagg tcatcaggat cggacgtaaa cgcatccgca ccgcatcaag cgtcgcttca
      361 atcagacctt taccttcttt atccatcaag tctttggcga attcgacgat aagtatcgcg
      421 ttcttcgccg acaacccaat ggttgtgagc agtcctactt ggaagtaaac gtcattggtc
      481 aggccacgga aggtggcagc cagcaacgca ccgataaccc ccagcggaac gaccagcata
      541 acggagaacg gagtcgacca gctctcgtac agcgccgcca gacacaggaa tacgacaatc
      601 aacgaaatcg cgtacagtga aggtgcctgg ttgccggaga gacgttcctg ataggacatc
      661 cccgtccagt catagccaac accggtaggc agtttgctcg ccagttgttc catcagctcc
      721 attgcttcac cggtactttt acccggtgcc gcctggccta agatttccat ggatggcagg
      781 ccgttgtaac gttccagacg cggcgaaccg tactcccaac gagaagagga gaacgccgag
      841 aatggcacca tctgaccatc agcagcacga acataccagt cgccgatatc atccggcagc
      901 atacggtatt tcgcttctga catgacgtaa actttcttca cacgaccgcg gtcgataaag
      961 tcgttcacat agctaccgcc ccatgcagcg cccagagtgg tgttaatgtc gttgatagaa
     1021 acacccagcg cctgcgcttt ttcctggtcg atatcaatct taaactgcgg ggtatcttcc
     1081 agaccgtttg gacgtacgct ggtcagcata tcagggtgct tcgctgcttc tgcaagcagc
     1141 tggttacgcg cctgagtcag tttttcgtga ccaaggccag cctggtcaat cagctcaaag
     1201 tcaaagccgg ttgcagtacc cagttccacg attgcgggca ggttaaaggc gaaaaccatc
     1261 gcatctttga tttgcgagaa agcgcgtgtt gcacgcatgg taatcgcttc aactttgttt
     1321 tcttcgcccg gacgatcggc ccagtccttc aaggaaacga acgcaatacc ggtattctga
     1381 ccacgtcccg caaagccgaa gccgttaacg gcgaacaccg actcaacgtt gttcttttct
     1441 ttggtcagat agtaatgcgt tacctcattg agcactttct gcgtacgttc ctgcgttgca
     1501 cctgctggca gctgaaccat ggtcataaat acgccctggt cctcatctgg caagaaggag
     1561 cttggcagac gcacgaacag ataggccatg ccgaccacga tgatcagata cagcactagg
     1621 taacgccccg tactgcgcag aataccgcct acgctgtcgg tgtagtggtg cgtgctcttc
     1681 tcgaacatgc ggttcaacca gccgaagaag ccttttttac cttccccgtg atcgcctttg
     1741 gcaatcggtt tcagcatggt ggcacaaaga gccggagtca ggatcaacgc taccagtacc
     1801 gacagcgcca ttgctgaaac aatggtaata gagaactgac gatagatagc accagtagaa
     1861 ccgccaaaga aggccatcgg tacgaatacc gccgacagta ccatcgcgat accgaccaga
     1921 gcgccctgaa tctgccccat cgacttacgg gtagcttctt ttggcggcaa accttcttcc
     1981 gccataacac gctccacgtt ttctaccaca acgatagcgt catccaccaa caagccgatg
     2041 gcgagcacca tcccgaacat tgttagcgtg tttatcgaga agccaaaggc ggcaaggacg
     2101 gcaaaggtcc cgagcaatac caccggtacg gcaatggtcg gaatcaacgt cgcgcggaag
     2161 ttctgcagga acagatacat aaccaggaac acgaggatga tcgcttcgac cagcgtttta
     2221 accacttcgt gaatagagat tttcacgaac ggcgtggtgt cgtacgggta aacaattttc
     2281 agacccgacg ggaagaacgg ttccatcttc gccagttcag cacggattgc cgcagcggta
     2341 tccagcgcgt ttgcaccggt cgccagcttg atccccagac cggaagcagg ttggccgtta
     2401 aactctgcga tgatgtcgta gttctcacca cccagctcaa tcttcgccac atcacgcagc
     2461 agtacgcggg aaccatcctg attcactttc agcaggattt tgccgaactc ttcagtagag
     2521 gtcagacgcg tctgagcaat aatagaggcg ttaagctgtt ggcctttcac cggcggcgta
     2581 ccaccgagct gacccgccgc aacctgggcg ttctgcgctt tgatggcggt aatgacatca
     2641 accggcgtta gctggaattt gttcagctca ttcgggttca tccagatacg catcgcgtac
     2701 tgtgaaccga acaactgaac atcacccacg cccgacgtac ggctgatggc atctttcata
     2761 ttcgccgcta cgtagtcaga gatatcctcc tgcgtcatgg tgccatcggt gttgataacg
     2821 ccgacaacca tcaggaagct gctggatgat ttctcaacgc tcaccccttg ctgctgaact
     2881 tcttgcggca gcaacggcat cgccagctgc agtttgttct gcacctgaac ctgcgcgata
     2941 tccgcatcag taccagactc aaaggtcagg gtgatctgca cggtacccgt ggagtcactg
     3001 ttagaggaca tgtacatcag gttatcgata ccgttcatat tctgttcgat aacctgtgtc
     3061 accgtgtcct gcactgtttt cgcatcagcg ccggggtagg aggcggagat cgttactgcc
     3121 ggcggtgcaa tcgtaggata ttgcgccacc ggcagtttga ggatcgccag cccccctgcc
     3181 aacatgatga taatggcgat cacccacgca aaaatcgggc gatcgataaa gaaattaggc
     3241 atgtcttaac ggctcctgtt taagttaaga cttggactgt tcaggctgag caccgcttgc
     3301 ggcttgctgg ttattatcag cggtaacttc ttgtgctttt acctggacac caggacgcac
     3361 tttctgcagc ccacttatta ctacgcgatc gcctgctttc agaccttctg tcaccagcca
     3421 cttatcgcca atagcctggc ttgcaacgat cggacgggtt tccactttgt catccgcgcc
     3481 aactaccagt acggtggcat caccacgcgg cgtacgggtt acgccctgtt gcgggactaa
     3541 aatagcgttt ggattaagcc cttcttccag acgtgcgcgc acgaacatac ccggcagcag
     3601 agtgtgatcc gggttcggga agatagcgcg tagggtgata gacccagtgg tctgatcaac
     3661 ggtaacgtca gagaattcca gcgtaccgtc ctgcgggaac ttaatgccgt cactggtgat
     3721 cagcgacact ttggctttgc cgttctcttg tttcagcgtg ccattcgcca gttcctgttt
     3781 caggcgaagg aagtcgttgc tggactgggt cacatcaacg tagatcggat caagttgctg
     3841 cacggttgcc agcacagtcg cctgaccgtt ctgtaccaat gcgccttccg tcacgttcga
     3901 cttaccaatg cgaccgctaa tcggagaggt gactttggtg taagccagat tgatccgcgc
     3961 agtttcaacg gcagctttcg ccgcagttac cgcagcattc gcctgttgcg catcagccag
     4021 agcctgatcg tacccttgct tactgatgta ctgagtaccg agcagtttct gataacgatt
     4081 caccgtcaat tgcgcgatat tggctgcagc ctgggctttc gccagatcac ctttcgcact
     4141 gtcgtatgtc gcctgatagg tcgcaggatc aatctgatag agagagacac cagcttcgat
     4201 gtcgctacct tctttgaaat tacgcttcag gataatcccg ctaacttgag gacgaacttc
     4261 tgcgatccgg taggcactgg tgcgacctgg aagctcggtt gtgatctgca gaggttcagt
     4321 tttgactgtt actacgccaa cggcgggcat ctgctggcca ccttgttggg cctgtttgtc
     4381 gtcacatcct gttagggcta agctgcctga gagcatcaga acgaccgcca gaggcgtaaa
     4441 ccctctgttt ttgttcatat gtaaacctcg agtgtccgat ttcaaattgg tcaatggtca
     4501 aaagttaata aacccattgc tgcgtttata ttatcgtcgt gctatggtac atacattcac
     4561 aaatgtatgt aaatctaacg cctgtaaatt cacgaacata tggcacgaaa aaccaaacaa
     4621 gaagcgcaag aaacgcgcca acacatcctc gatgtggctc tacgtctttt ctcacagcag
     4681 ggggtatcat ccacctcgct gggcgagatt gcaaaagcag ctggcgttac gcgcggtgca
     4741 atctactggc attttaaaga caagtcggat ttgttcagtg agatctggga actgtcagaa
     4801 tccaatattg gtgaactaga gcttgagtat caggcaaaat tccctggcga tccactctca
     4861 gtattaagag agatattaat tcatgttctt gaatccacgg tgacagaaga acgacgtcga
     4921 ttattgatgg agattatatt ccacaaatgc gaatttgtcg gagaaatggc tgttgtgcaa
     4981 caggcacaac gtaatctctg tctggaaagt tatgaccgta tagaacaaac gttaaaacat
     5041 tgtattgaag cgaaaatgtt gcctgcggat ttaatgacgc gtcgggcagc aattattatg
     5101 cgcggctata tttccggcct gatggaaaac tggctctttg ccccgcaatc ttttgatctt
     5161 aaaaaagaag cccgcgatta cgtcgccatc ttactggaga tgtatctcct gtgccccacg
     5221 cttcgtaatc ctgccactaa cgaataaccc tgaatctgac tccaggattt ttcctggaca
     5281 ttttcgtcgt tgctattctg gttcactgcg tcgtgatatt cttgcggttt gactttttca
     5341 ggtcgttctt caggttcaga aaccttcatt catcatgact atgttccagt attacaaacg
     5401 atcacggcat tttgtttttt cagcatttat tgcttttgtt tttgtcttgt tatgccagaa
     5461 cacggcgttt gcgcgggcgt catcgaatgg tgatctgccg acaaaagcgg acctgcaggc
     5521 gcaacttgac tcactaaata aacaaaaaga tctttctgct caggacaaac tggtgcagca
     5581 ggatctgaca gatacattag ccaccctcga taaaatcgat cgcataaaag aagagacagt
     5641 tcagctacgg caaaaagtcg ctgaagcgcc ggaaaaaatg cgccaggcga ccgcggcgtt
     5701 aacagcactt agcgatgtcg ataacgacga agaaacgcgc aaaattctga gtacgctttc
     5761 gttgcgccag ctggaaactc gcgttgccca ggcgctggac gatttgcaaa acgcacaaaa
     5821 cgatctggcg tcttataaca gccagctggt ttcgttacag acgcagcccg aacgcgtgca
     5881 aaatgcgatg tataacgctt cgcagcagct gcaacaaatt cgcagtcgtc tggatgggac
     5941 tgatgtcggc gagactgcct tacgtcccag ccagaaagtg ttaatgcagg ctcagcaggc
     6001 gttgctgaat gcggagattg accagcagcg taaaagcctg gaagggaaca ccgtcttgca
     6061 ggataccttg caaaagcaac gtgattacgt gacggcgaac agcgcccgtc tggagcacca
     6121 gttgcagctg ttgcaagaag cggtaaacag caagcgcctg actttaactg aaaaaacggc
     6181 gcaggaagcc gtctccccgg atgaagccgc gcgtattcag gctaatccgc tggtgaaaca
     6241 ggaactggaa attaaccagc agttaagtca gcgtctgatt accgcgactg aaaacggtaa
     6301 tcagttgatg cagcaaaaca ttaaagtcaa aaactggctg gagcgggcgc tgcaatcgga
     6361 acgaaatatt aaagagcaga ttgccgtcct gaagggcagc ctgctgttgt ctcgtatcct
     6421 ttaccagcaa caacaaacgc tgccctcggc ggatgaactg gaaaacatga ccaaccgcat
     6481 cgcggatttg cgtctcgaac agtttgaagt taaccagcag cgtgatgcac tcttccagag
     6541 cgatgcgttc gtcaacaaac tggaagaagg tcacaccaac gaagtcaaca gcgaagttca
     6601 cgatgcgtta ttgcaagtgg ttgatatgcg tcgcgaattg ctggatcaac tcaacaaaca
     6661 gttgggtaac cagctgatga tggccattaa cctgcaaatc aaccagcagc agttaatgag
     6721 cgtatcgaaa aacctgaaat ccatcctgac tcagcaaatc ttttgggtga acagtaaccg
     6781 tccaatggac tgggactgga tcaaagcgtt cccgcaaagc ctgaaagatg aatttaagtc
     6841 gatgaaaatc acggtgaact gggaaaaagc ctggcctgcc gtttttatcg ctttcctcgc
     6901 cggtttgccg ctgctgttga ttgccgggct gatccactgg cgtctgggct ggctgaaagc
     6961 gtatcaacag aaactggctt ccgctgtggg ttccctgcgt aacgatagcc agctcaacac
     7021 accaaaagcg atccttatcg acctgatccg cgcgctgccg gtgtgcctga ttattctcgc
     7081 ggttggcctg attctgttga ccatgcagct caacatcagc gaactgctat ggtcgttcag
     7141 taaaaaactg gcgattttct ggctggtgtt tggcctgtgc tggaaggtac tggagaaaaa
     7201 tggcgttgcc gtgcgtcact tcggcatgcc ggagcagcag accagccact ggcgtcggca
     7261 aattgtccgc atcagtctcg cgttgctgcc tatccatttc tggtctgtgg tggcagaact
     7321 ttccccgctg catctgatgg atgatgtgct ggggcaggcg atgattttct tcaacctgct
     7381 gctgattgcc ttcctggtat ggccaatgtg ccgcgaaagc tggcgtgata aagagtcgca
     7441 caccatgcga ctagtcacca ttaccgtgct gtcgattatc ccgattgcat tgatggtgct
     7501 gactgccacg ggttatttct acaccacgct gcgcctctcc gggcgttgga ttgaaaccgt
     7561 ttatctggtg atcatctgga acttgctcta ccagacggta ttgcgcggct taagcgtagc
     7621 ggcgcggcgt atcgcctggc gtcgtgcgct ggcgcgtcgg cagaatctgg tgaaagaggg
     7681 cgcagaaggt gccgaaccgc cggaagaacc caccattgca ctggagcaag ttaaccagca
     7741 gacgctgcgt attaccatgt tgctgatgtt tgcgctgttc ggtgtcatgt tctgggcaat
     7801 ttggtccgat ttgatcaccg tgttcagcta tctcgacagc atcacgctct ggcattacaa
     7861 cggcactgaa gctggcgctg cggtggtgaa aaacgtcacc atgggcagtc tgttgtttgc
     7921 gattatcgcc tcaatggtgg cctgggcgtt gattcgcaac ctgcctggtt tactggaagt
     7981 gctggtgctc tcgcgactga atatgcgcca gggcgcgtcg tatgccatta ctaccatcct
     8041 taactacatc attattgccg ttggtgcgat gacggtgttc ggatcgctgg gcgtctcttg
     8101 ggataaactc cagtggctgg ccgcagcatt atccgtaggt cttggttttg gtttacaaga
     8161 aattttcggt aacttcgtct ccggtttgat cattctgttc gaacgtccgg tgcgtattgg
     8221 cgatacggta accattggta gcttctcggg gacggtaagt aagatccgta ttcgtgcgac
     8281 aacgattacc gatttcgatc gcaaagaagt gatcatcccg aacaaagcgt ttgttaccga
     8341 gcgtctgatc aactggtcgt tgactgacac tactacgcgt ctggtgatcc gtctcggcgt
     8401 ggcctatggc tccgatctgg aaaaagtgcg taaagtgtta ctgaaggcgg cgactgagca
     8461 cccaagggtg atgcacgaac caatgccgga agtcttcttt acggcatttg gtgccagcac
     8521 gttggatcat gagctgcgtc tgtatgtgcg tgaactgcgt gaccgtagcc gtactgtcga
     8581 tgagctgaac cgtactatcg atcagctgtg ccgtgaaaac gacatcaaca ttgcctttaa
     8641 ccagcttgaa gtgcatctgc acaacgagaa gggcgatgag gtgacggaag taaaacgcga
     8701 ctacaaaggc gacgatccga caccagcggt tgggtaataa ccaaaggggg cgacatttag
     8761 ttgccccgag attactgaca aagtgcgcgt tgttcatgcc ggatgcggcg tgaccgcctt
     8821 atccggccta cgaaaccgca agaattcaat atattgcagg agcggtgtag gcctgataag
     8881 cgtagcgcat caggcagttt tgcgtttgcc cgcaatctta ggggacattt agcggcccca
     8941 tttatttctc acttttccgc ctcatcatcg tgcgttaatt tcttttcata atcacgcttt
     9001 acaatatcca gcgcgcgcag cacggtactg gcagggatct gattttcctc cagcagcaca
     9061 atcaaatcga cagccagttt gacatcgtca ggggcatttt caagtgacat actctttcca
     9121 ttgctagcgg gttaagcgcg ctagcctgtt ttcgattttt tccagcgcat ggcggcagcg
     9181 tgccaggcgc gcttcatagg cttccacttc acgatgcagc gtttgctgtt ccacgagatc
     9241 ggtcacccgc gccaggcggg ctctgcgttc ggcaaccatc tcacgtagcc gccgctcaaa
     9301 atcctgatgc tgaatacgtt tacgctgcca gcgggaaatt ttcggtggcg cactgtccca
     9361 ctcgcgcaat gaccaggcgg aggcttcacg cgcgatggct tccagttgtg ccgccagatg
     9421 ttccgccagc caggccactt gcggcagctg ttgctgctca actgcatgac gaagcgcagc
     9481 cagattatcg cccgcctcgt cgagacaaga ctgtagtgtt gtcgcacgag tctgaaaaag
     9541 atgcctgtcg aaacgagcac ttagcgtggc gaactgtgac accggggcac aacgctgacg
     9601 cagcgtagcg agctgtcctt ccagtttttc cagcagcagg gcggttttca cgataagacc
     9661 tcaatgaaaa tgataattgt tatgctaaag tagccactat gtaagctgac aacactcaat
     9721 atgcaacgaa tcattttaat catcattggc tggctggcgg tagtgctggg tacgctgggc
     9781 gtggtattac cggtattacc gacgacgccg tttatcctgc tggcggcctg gtgctttgcc
     9841 cgttcttccc cgcgctttca cgcctggttg ctgtaccgct catggtttgg cagctatcta
     9901 cgtttctggc agaaacatca tgcgatgccg cgcggcgtca aaccgcgggc gattttgctt
     9961 attttgctca cgtttgccat ttctctgtgg ttcgtccaga tgccatgggt gcgcatcatg
    10021 ttgctggtaa ttctcgcctg tttgcttttc tatatgtggc gaattccggt gattgatgaa
    10081 aagcaagaaa agcactgaag cacaacaatc gcagttgcaa ttattgcgta cagccagtac
    10141 attctggcgt tttcgagcac aggcgcaggc ggtcaaaggt taaacaacag ttacttttga
    10201 tacgtttaaa acgcgccgtg agtaccaccg taacaagcag gcatacactt atgaccgcga
    10261 ctgcacagca gcttgagtat ctcaaaaata gcatcaaaag cattcaggac tacccaaaac
    10321 ccggcattct tttccgcgat gtcaccagct tactggaaga cccgaaagct tacgctctca
    10381 gcatcgactt gctggttgag cgttacaaaa atgcgggcat taccaaagtt gtcggcaccg
    10441 aagcgcgtgg cttcttgttt ggcgctccgg tagctctggg tctgggcgtt ggctttgtac
    10501 cggtccgtaa accgggcaaa ctgccgcgtg aaaccatcag tgaaaattac gacctggaat
    10561 acggcaccga tcagctggaa atccacgttg atgccatcaa accgggcgac aaagttctgg
    10621 tggtggacga tctgctggca accggcggca ctatcgaagc gaccgttaaa ctgatccgtc
    10681 gtctgggtgg tgaagtggct gacgctgcgt tcattatcaa cctgtttgat ctcggcggcg
    10741 aacagcgtct cgaaaaacag ggcattacca gctacagcct tgtcccgttc ccgggccatt
    10801 aattatcgcc agtcttgtgc tgcccacgct acggacagca caagatgtgc attcagcctc
    10861 gccgttctga cggggctgtg ttagcattac cccttcgtga atccaccttc cagcgtttca
    10921 gagcctgcca atgagttatc aggttttagc ccgaaaatgg cgcccacaaa cctttgctga
    10981 cgtcgtcggc caggaacatg tgctgaccgc actggcgaac ggcttgtcgt tagggcgtat
    11041 tcatcatgct tatctttttt ccggcacccg tggcgtcgga aaaacctcta tcgcccgact
    11101 gctggcgaag gggctaaact gcgaaaccgg cattaccgcg acgccgtgcg gcgtgtgcga
    11161 taactgtcgt gaaatcgagc aggggcgctt tgtcgatctg attgaaatcg acgccgcctc
    11221 gcgcaccaaa gttgaagata cccgcgacct gctggataac gtccagtacg ctccggcgcg
    11281 tggtcgtttc aaagtttatc tgatcgacga agtgcatatg ctgtcgcgcc acagctttaa
    11341 cgcactgtta aaaacccttg aagagccgcc ggagcacgtt aagtttctgc tggcgacgac
    11401 cgatccacag aaattgccgg tgacgatttt gtcacgctgt ctgcaatttc atctcaaggc
    11461 gctggatgtc gagcaaattc gccatcagct tgagcacatc ctcaatgaag aacatatcgc
    11521 tcacgagccg cgggcgctgc aattgttggc gcgcgccgct gaaggcagcc tgcgagatgc
    11581 cttaagtctg accgaccagg cgattgccag cggtgacggc caggtttcaa cccaggcggt
    11641 cagtgcgatg ctgggtacgc ttgacgacga tcaggcgctg tcgctggttg aagcgatggt
    11701 cgagggcaac ggcgagcgcg taatggcact gattaatgaa gccgctgccc gtggtatcga
    11761 gtgggaagcg ttgctggtgg aaatgctcgg cttgttgcat cgtattgcga tggtacaact
    11821 ttcgcctgct gcacttggca acgacatggc cgccatcgag ctgcggatgc gtgaactggc
    11881 gcgcaccata ccgccgacgg atattcagct ttactatcag acgctgttga ttggtcgcaa
    11941 agaattaccg tatgcgccgg accgccgcat gggcgttgag atgacgctgc tgcgcgcgct
    12001 ggcgttccat ccgcgtatgc cgctgcctga gccagaagtg ccacgccagt cctttgcacc
    12061 cgtcgcgcca acggcagtaa tgacgccaac cctggtgccg ccgcaatcag cgccgcagca
    12121 ggcaccgact gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc gccagcagtt
    12181 gcagcgcgtg cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc
    12241 gcggccggtg aataacgctg cgctggaaag actggcttcg gtcaccgatc gcgttcaggc
    12301 gcgtccggtg ccatcggcgc tggaaaaagc gccagccaaa aaagaagcgt atcgctggaa
    12361 ggcgaccact ccggtgatgc agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa
    12421 agcgctggaa catgaaaaaa cgccggaact ggcggtgaag ctagcggcag aagccattga
    12481 gcgcgacccg tgggcggcac aggtgagcca actttcgcta ccaaaactgg tcgaacaggt
    12541 ggcgttaaat gcctggaaag aggagagcga caacgcagta tgtctgcatt tgcgctcctc
    12601 tcagcggcat ttgaacaacc gcggtgcaca gcaaaaactg gctgaagcgt tgagcatgtt
    12661 aaaaggttca acggttgaac tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc
    12721 gctggagtgg cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg agtccattat
    12781 tgcggataat aatattcaga ccctgcgtcg gtttttcgat gcggagctgg atgaagaaag
    12841 tatccgcccc atttgatcgt aagcacagct tacgttcgtc atccttaacg tgattgagag
    12901 agaaacctat gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc
    12961 aagaaaaaat gcagaaaatg caggaagaga tcgcgcagct ggaagttacc ggcgaatctg
    13021 gcgcaggtct ggtaaaagtg accatcaacg gtgcacacaa ctgccgtcgc gtagagatcg
    13081 acccgagcct gctggaagac gacaaagaga tgctggaaga cctggtggct gcagcattca
    13141 acgacgcagc acgtcgtatt gaagaaacgc agaaagaaaa aatggcctct gtatcctccg
    13201 gaatgcagct gccgcctggc tttaagatgc cgttctgatg caaaccagcc cgctgttaac
    13261 acagcttatg gaagcactgc gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat
    13321 ggcgttcacg ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac
    13381 ccgggcgatg tcggaaatcg gccactgcgc cgattgccgc actttcaccg aacaggaagt
    13441 ctgtaacatc tgttcgaatc cgcgtcgtca ggaaaacggt caaatctgtg tggtggagag
    13501 tccggcggac atctacgcca ttgagcagac ggggcagttt tctggtcgtt attttgtgtt
    13561 gatggggcat ctgtcaccgc tggacggcat cggtccggat gatatcgggc ttgatcgtct
    13621 ggaacagcgt ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca atcccacggt
    13681 tgaaggtgaa gctaccgcta actacattgc cgagctttgc gcgcaatatg acgtggaagc
    13741 cagccgaatc gctcatggcg taccggttgg cggcgaactg gaaatggtcg atggcaccac
    13801 gctgtcacac tccctcgccg ggcgtcataa gattcgtttt taagcaaacg agagcaggag
    13861 cacctgctct cgcttgaaat tattctccct tgtccccatc tctccctcat cctgttttta
    13921 accttaaaat ggcattattg aggtagacct acatgaaagg acaagaaact cgtggttttc
    13981 agtcagaagt gaaacagctt ctgcacctga tgatccattc tctctattcc aataaagaaa
    14041 tcttcctgcg tgagcttatc tctaacgcct ccgatgcggc ggacaagctg cgtttccgtg
    14101 cgctatctaa cccggacctg tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg
    14161 ataaagacaa gcgcacgctg accatctccg ataacggcgt ggggatgacc cgcgacgaag
    14221 tgattgacca tctggggact atcgctaaat ccggtaccaa atcattcctc gaatccctgg
    14281 gttctgacca agcgaaagac agccagctga tcggtcagtt tggtgttggt ttctactctg
    14341 cgtttatcgt ggccgacaaa gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa
    14401 acggcgtctt ctgggaatcg gctggcgaag gtgaatatac cgttgccgat atcaccaaag
    14461 aagatcgtgg tactgaaatc accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg
    14521 actggcgcgt gcgttccatc atcagcaaat actccgacca tatcgcgctg ccggtagaga
    14581 tcgaaaaacg cgaagagaaa gatggcgaaa ccgttatctc ctgggcaaaa atcaacaaag
    14641 cgcaggcgct gtggactcgt aacaagtcgg aaatcaccga tgaagagtat aaagagttct
    14701 acaaacacat cgcccacgac tttaacgatc cgctgacctg gagccacaac cgcgttgaag
    14761 gtaagcagga gtacaccagc ctgctgtaca tcccgtccca ggctccgtgg gatatgtgga
    14821 accgtgatca taaacacggc ctgaaactgt acgttcaacg cgtgtttatc atggacgacg
    14881 ctgaacagtt catgccgaac tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc
    14941 tgccgctgaa cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca
    15001 atgcgctgac caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaaa
    15061 aataccagac cttctggcaa cagtttggcc tggtactgaa agaaggtccg gcggaagatt
    15121 tcgctaacca ggaagcgatc gccaaactgc tgcgttttgc ttctacttat accgattctt
    15181 ctgcgcagac cgtgtctctg gaagactacg tttcccgcat gaaagaaggg caggagaaaa
    15241 tctactacat cactgcagac agctatgcgg cagcgaagag cagcccgcac ctggaactgc
    15301 tgcgtaagaa aggcatcgaa gttctgctgc tttccgaccg catcgatgag tggatgatga
    15361 actatctgac tgagttcgac ggtaaaccgt tccagtcggt gtctaaagtt gacgagtcgc
    15421 tggaaaaact ggctgacgaa gttgatgaga gcgcgaaaga agcggagaaa gcactgactc
    15481 cgttcatcga ccgcgtgaaa gctctgctcg gcgagcgcgt gaaagatgtc cgtctgactc
    15541 accgtctgac cgatacgcca gcgatcgttt cgaccgacgc ggacgaaatg agcactcaga
    15601 tggcgaaact gttcgctgcg gcgggccaga aagtgccaga agtgaaatac atcttcgaac
    15661 tgaacccgga tcacgtactg gtgaaacgtg cggcagatac tgaagatgaa gccaagttca
    15721 gcgagtgggt agaactgctg ctggatcagg cgctgctggc agaacgcggc acgctggaag
    15781 atccgaacct gtttattcgt cgtatgaatc agctgctggt ttcctgatgt catgccggat
    15841 gacctttgtg tcatccggca tttttctttt catcatctgt actttccgca aattatctcg
    15901 ccattaaccg tttcagcctc aggtgccttt cttgaggcaa tcgcctgttg gtggtatcgt
    15961 ttatcgcttt ttcaaaaaaa tcgacacatt ttaaggggat tttcgcaatg cgtatcattc
    16021 tgcttggcgc tccgggcgcg gggaaaggga ctcaggctca gttcatcatg gagaaatatg
    16081 gtattccgca aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc
    16141 tgggtaaaca agcaaaagac attatggatg ctggcaaact ggtcaccgac gaactggtga
    16201 tcgcgctggt taaagagcgc attgctcagg aagactgccg taatggtttc ctgttggacg
    16261 gcttcccgcg taccattccg caggcagacg cgatgaaaga agcgggcatc aatgttgatt
    16321 acgttctgga attcgacgta ccggacgaac tgattgttga tcgtatcgta ggccgccgcg
    16381 ttcatgcgcc gtctggtcgt gtttatcacg ttaaattcaa tccgccgaaa gtagaaggca
    16441 aagacgacgt taccggtgaa gaactgacta cccgtaaaga cgatcaggaa gaaaccgtgc
    16501 gtaaacgtct ggttgaatac catcagatga ctgcaccgct gatcggctac tactccaaag
    16561 aagcggaagc gggtaacacc aaatacgcga aagttgacgg taccaagcag gttgctgaag
    16621 ttcgcgctga tctggaaaaa atcctcggct aatcaagccg cagcagatct gctttcgggc
    16681 aggtctgctt atccacctca ggcaatacct ctcacagcaa ttagttcttc ttcctcactt
    16741 ttccgctaca attatcaaca agttgaatcg ataagaggcg gtaatgcgtc agactaaaac
    16801 cggtatcctg ctggcaaacc tgggtacgcc cgatgccccc acacctgaag cggtaaaacg
    16861 ctatctgaaa caatttttaa gcgacagacg cgtggttgat acctcacggt tgttatggtg
    16921 gccgttgctg cgcggcgtga ttttgccgct gcgctcgccg cgtgtggcga agctgtacgc
    16981 ctctgtctgg atggaagacg gctcgccgct gatggtttac agccgccagc aacagcaggc
    17041 gttggcacaa cgtttaccgg atacgccagt ggcgctgggc atgagctacg gctcgccatc
    17101 actggaaagc tccgtcgatg aactcctggc agagcatgta gatcatattg tggtgctgcc
    17161 gctttatccg caattctcct gttctacggt cggtgcggta tgggatgaac tggcacgcat
    17221 tctggcgcgc aaacgtagca ttccggggat atcgtttatt cgtgattacg ccgataacca
    17281 cgattacatt aatgcactgg cgaacagcgt acgcgcttct tttgccaaac atggcgaacc
    17341 ggatctgctg ctgctctctt atcatggcat tccccagcgt tatgcagatg aaggcgatga
    17401 ttacccgcaa cgttgccgca caacgactcg cgaactggct tccgcactgg ggatggtacc
    17461 ggaaaaagtg atgatgacct ttcagtcgcg ctttggccgg gaaccctggc tgatgcctta
    17521 taccgacgaa acgctgaaaa tgctcggaga aaaaggcgta ggtcatatac aggtgatgtg
    17581 cccgggcttt gctgcggatt gtctggagac gctggaagag attgccgagc aaaaccgtga
    17641 ggtcttcctc ggtgcgggcg ggaaaaaata tgaatatatt ccagcgctta atgccacgcc
    17701 ggaacatatc gaaatgatgg ctaatcttgt tgccgcgtat cgctaaagct gagcggtaaa
    17761 gaactgagcg ccgttgcgaa gagcctcgtc ggcggttttc atcatccgtg aataatgcaa
    17821 aaaggcatgc agcgtacctg ggtagagttt gaactcacag ggctgctgat gcgccgctaa
    17881 cgtctagtaa agcagacggc tgtcatccag cagcggatcg aactccgccc cggcaataaa
    17941 acagggcgga acttcgcgag tgagatcatt attaaacaga cagtaatacg gtgactcgcg
    18001 gtccgcgtcg ttgcttaaat acgcctcttc atacatctgc aaatcctgtt gcgttaagcc
    18061 atcccagaca ccgcccaaca gacgacgagt cacggaatcc cgtaatccgt aaagcccata
    18121 ccacagcaaa acacccgcaa ttttaccgca atcgatctgt ttatcacgca accacaacgc
    18181 actggcgagc gccagcatgg cacctgcgga atcaccggca aagccaatgc gggacatatt
    18241 gatttgataa tcctccgcct gctggtggaa ataacaacaa gcagccacaa tttcctctat
    18301 cgcttgtgga aaacgcgctt caggtgaaag ggtgtaatta ataccaatca ccgtacattg
    18361 gctgtagctt gccagcaggc gcatgatgcg atcgtgggta tcgagattgc cgagaataaa
    18421 accgcctcca tgcaaataaa atagcgtcgc cgggctatct ggctgcggac aaaagagacg
    18481 tgtttccacc tgcccatatt ttgttggaac catgtaagct ctggttgcca tttctggagc
    18541 gcccgcattc cagaatcggc gctcaagcgt gtaatactgt cgttgctcag caatcgttcc
    18601 cgttgcgggc caggacggta aatccggctg aagagtattc acaacggtct tcatttcagc
    18661 agaaataagg tccagaacag gtagtttgtt ttccggcttc atacaaaaac tcctttcaaa
    18721 ttacgtcatt gtaaggaacc actgccatga aaatgcgatc ccgcctgctg atattgaaac
    18781 tggctgcgtc tcgcgcgctc ccgtcagatt gtgttaacat tcgccgctca gttaaccacc
    18841 cgtaaaaaca accatgaaat ttcccggtaa acgtaaatcc aaacattact tccctgtaaa
    18901 tgcacgcgat ccgctgcttc agcaattcca gccagaaaac gaaaccagcg ccgcctgggt
    18961 cgtaggtatt gaccagacgc tggtcgatat tgaagcgaaa gtggatgatg aattcattga
    19021 gcgttatgga ttaagcgccg ggcattcact ggtgattgag gatgacgtag ccgaagtgct
    19081 ttatcaggag ctaaaacaga aaaacctgat tacccatcag tttgcgggtg gcaccattgg
    19141 taacaccatg cacaactact cggtgctcgc ggacgaccgt tcggtgctgc tgggcgtcat
    19201 gtgcagcaat attgaaattg gcagttatgc ctatcgttac ctgtgtaaca cttccagccg
    19261 taccgatctt aactatctac aaggcgtgga tggtccgatt ggtcgttgct ttacgctgat
    19321 tggcgagtcc ggggaacgta cctttgctat cagtccaggc cacatgaacc agctgcgggc
    19381 tgaaagcatt ccggaagatg tgattgccgg agcctcggca ctggttctca cctcatatct
    19441 ggtgcgttgc aagccgggtg aacccatgcc ggaagcaacc atgaaagcca ttgagtacgc
    19501 gaagaaatat aacgtaccgg tggtgctgac gctgggcacc aagtttgtca ttgccgagaa
    19561 tccgcagtgg tggcagcaat tcctcaaaga ccacgtttct atccttgcga tgaacgaaga
    19621 tgaagccgaa gcgttgaccg gagaaagcga tccgttgttg gcatctgaca aggcgctgga
    19681 ctgggtagag ctggtgctgt gcaccgccgg gccaatcggc ttgtatatgg cgggctttac
    19741 cgaagacgaa gcgaaacgta aaacccagca tccgctgctg ccgggcgcta tagcggaatt
    19801 caaccagtat gagtttagcc gcgccatgcg ccacaaggat tgccagaatc cgctgcgtgt
    19861 ctattcgcac attgcgccgt acatgggcgg gccggaaaaa atcatgaaca ctaatggagc
    19921 gggggatggc gcattggcag cgttgctgca tgacattacc gccaacagct accatcgtag
    19981 caacgtacca aactccagca aacataaatt cacctggtta acttattcat cgttagcgca
    20041 ggtgtgtaaa tatgctaacc gtgtgagcta tcaggtactg aaccagcatt cacctcgttt
    20101 aacgcgcggc ttgccggagc gtgaagacag ccaggaagag tcttactggg atcgttaagt
    20161 tatcgtcggt tcgtaggcca gataaggcgt tcacgccgca tctggcattt ggctctcgat
    20221 gcctgatgcg acgctggcgc gtcttatcat gcctacatat ttttcatatt ttacatccgg
    20281 caaccaccgt ttaccccgtc accacttcac ccgccggtgg cgtttccagc agttccagca
    20341 tagtacgggc aatttcacgt tcacccatca ctacctgatt cgcgccacgt tcggtgatat
    20401 acgtcacttc atcgtcataa tgggcgcggg cgataatctc aatatccggg tttttcgcgc
    20461 gggcagacgc cacaatctca cccgcttcat aaccgttggg gatcgtcagg atcagccatt
    20521 ttgcacattc cagatgtgcc agttgcataa tttcttcgtt cgctgcattg cccaatacag
    20581 cgcggacccc acgctctcgc agctcatcaa cacgggttcg tgacgtctca atcaccacca
    20641 gcggaatatc agaggcgagc aatttctccc ccagcaggct gcctacacga ccgtaaccca
    20701 ccaatagcgc atggttgcaa atatccactg ggatctgttt ctcttcttcg attgcctctt
    20761 ccagcgtctg ctcttccagc gtttcggtct tcgccagata tttctccagt agtgcgaaca
    20821 gtaccgggtt gagcataatc gacaggatcg cccctgccag taccaggttt tgtccggcct
    20881 gcggcagtaa attcaatgcc attcccagtc ccgccaggat aaacgcgaac tcaccaatct
    20941 gcgccaggct ggcggcgatg gttaatgccg tacgttggga gtgaccaaac agacggacaa
    21001 ggaacagggc cgcgaccgat ttaccaaaca gaataattgc cagcgtcgcc agcactgcca
    21061 gcggttgctg aatcagaatt aacggatcaa acaacatccc gactgagaca aaaaacagca
    21121 ccgcaaacgc gtcgcgcaat ggcaacgtat cgtgggcggc acggtgactc aattcagact
    21181 cgttcagtac catcccggca aagaacgcac cgagtgcaaa ggagacatca aacagctcta
    21241 ccgcaccaaa ggcaatccct aacgccagcg ccagcactga cagggtaaac agctcgcgag
    21301 aaccggttgc cgcgctgcgt gccataatcc acggcaccag acggcgacct accagcatca
    21361 taatggcgat aaatgcgatc accttgccga tggtgatccc catatcgact gcaagagtgg
    21421 caaagcccac atcgccctgt tccatcattc ctgccactgc gggcagcaac accagcgtca
    21481 gaaccatcac caggtcttcc acaatcaacc aaccgatggc gatttgccca cgctgactgt
    21541 ctattaattg ccgttcttca agtgcgcgca gtaacaccac ggtactggcg gtggaaagac
    21601 ataagccgaa cacgataccg gtcattaacg accagcccag cacggcggag agcgccatac
    21661 ccagcagcgt cgccacggct atctgggcga tcgcgccggg aatggcgatg gactttaccg
    21721 ccatcaaatc cttgagcgaa aaatgcaaac cgacgccgaa catcagcaaa ataacgccca
    21781 gttcagccag ttccggcgca agcttggtat cggcaacaaa gcccggagtg aatggtcctg
    21841 ccagcacacc cgctaacaga tatcccacca gaggagaaat acgtagttta ttggccagca
    21901 tgccgaggat aaaggcgagc acaaggccgc caacaatggt ggtgataagc ggggtggcgt
    21961 gatgcattcc gtctcctttt cctggtggtt attgtccatt tttggctggg aaaaccaaaa
    22021 ttacaggtaa tagtttatga caatttcatt gatgatgttt atgaataatt gttgaatttt
    22081 gcagaaaaat ggaattagct gcaaaaaaag cacggttcgg aaaacagaag gcggtttaac
    22141 agaggaaagg cttatggcga ctgtaaaacg atgcagccaa agtttgcctt tggctgcaat
    22201 gaaatcagtc tttatgccgg ttatcaggca ggaatatggt caacatcccc aatagtggca
    22261 ggaaagcaca gattttatag actaactcga tgctggtgtg atcggcgata agccccagaa
    22321 ctgccgctcc cagacctccc atgccgaaag caaaaccgaa aaagagtcca gaaaccatac
    22381 cgatacgtcc cggaagtagc tcctgagcgt agaccagaat ggcagagaat gccgaagcga
    22441 ggataaatcc aataatcacc gttaaaaccc ccgtccagtg caggcgggcg tagggtaaaa
    22501 tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat cacatatttc cgtccaattt
    22561 tatcccctac aggcccgccg atcaccgtac ctgccgcaac ggcaaacagg aaggcaaaca
    22621 gatgaagctg ggcattctgg atagataatc cgaatttttg catcagataa aaggtgtaat
    22681 agctgctgat gctcgccata tagaaatatt tcgagaaaat gaggattaac agaatgctga
    22741 ctgccagtac cactttgttg cgcggcagtg gattgattat cgtcgctttg ggttttcctt
    22801 tattcattcg gtgctgtgcc gagtaccaac ggctaatttg cgccaacacc acgatcgcca
    22861 gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggctcg ataatcaccg
    22921 ccgccagcaa gggtcccagg gaactgccaa agttgccgcc gacctgaaag atagattgcg
    22981 ccaggccatg ccgcccgccg gaagccatac gggctacgcg agaagattcc ggatgaaaga
    23041 ccgatgaacc ggtaccgacc agcgccgctg ccagcagaac tgcgccaaaa ctgcccgcca
    23101 gcgcaagcag caccagacca cttaaggtaa agcacatgcc aattggcaac gaccacggca
    23161 tcggatattt atcggtccag tagccgacca ctggttgcag tagcgaagag gcgagctgga
    23221 aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac tgaagcagcg
    23281 gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag aggctgatag
    23341 cacctaaaat accaaacgat gttcgggcct tggtcgttga cgcagccgcg cccgccacag
    23401 gctgggtttg ttcactcatt gccataggaa agtcactttt tcagggttgc gatgtaaaga
    23461 atgatcttat ttgtgattat tacccgacta acatacctgt atgcgtcgtc tgaaggaagt
    23521 ctcaacgccg aatacagaat ttctaatctg gatgcagatt tatcttcacc ggacgcagac
    23581 ttgtctatga tgttgcgtca tactattttt caacacgttg aaatcaggtc agggagagaa
    23641 gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt aaccacattt acactggcga
    23701 gtgaaactgc tctggcgtat gagcaggata aaacctacaa aattacagtt ctgcatacca
    23761 atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg gcggcgcaaa
    23821 aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc gtgctgctac
    23881 tttccggtgg cgacattaac actggcgtgc ccgagtctga cttacaggat gccgaacctg
    23941 attttcgcgg tatgaatctg gtgggctatg acgcgatggc gatcggtaat catgaatttg
    24001 ataatccgct caccgtatta cgccagcagg aaaagtgggc caagttcccg ttgctttccg
    24061 cgaatatcta ccagaaaagt actggcgagc gcctgtttaa accgtgggcg ctgtttaagc
    24121 gtcaggatct gaaaattgcc gttattgggc tgacaaccga tgacacagca aaaattggta
    24181 acctggaata cttcactgat atcgaatttc gtaagcccgc cgatgaagcg aagctggtga
    24241 ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc catatggggc
    24301 attacgataa tggtgagcac ggctctaacg caccgggcga tgtggagatg gcacgcgcgc
    24361 tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagatccg gtctgcatgg
    24421 cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa ccagatcaac
    24481 aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga cgggctgatt
    24541 ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg gtgaacctga
    24601 agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt gctttacact cctgaaatcg
    24661 ctgaaaacca gcaaatgatc tcgctgttat caccgttcca gaacaaaggc aaagcgcagc
    24721 tggaagtgaa aataggcaaa accaatggtc gtctggaagg cgatcgtgac aaagtgcgtt
    24781 ttgtacagac caatatgggg cggttgattc tggcagccca aatggatcgc actggtgccg
    24841 actttgcggt gatgagcgga ggcggaattc gtgattctat cgaagcaggc gatatcagct
    24901 ataaaaacgt gctgaaagtg cagccattcg gcaatgtggt ggtgtatgcc gacatgaccg
    24961 gtaaagaggt gattgattac ctgaccgccg tcgcgcagat gaagccagat tcaggtgcct
    25021 acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg caaactgaac gaccttaaaa
    25081 tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta aacttcaatg
    25141 ccaccggcgg tgatggatat ccgcgccttg ataacaaacc gggctatgtg aataccggct
    25201 ttattgatgc cgaagtgctg aaagcgtata tccagaaaag ctcgccgctg gatgtgagtg
    25261 tttatgaacc gaaaggtgag gtgagctggc agtaatccga aagtgccgga tgtttgcatc
    25321 cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt tggcatcgag gatctttgcc
    25381 agatcgcctg ccgccagttc gatatccagt ccgcgcttgc cgccggaaac ataaatagtg
    25441 gcaaattctt gtgcgggggc gtcgataatc gttggcagac gttttttctg ccccagtggg
    25501 ctaatccccc caaccaggta tcccgtcgaa cgctgcgcga ccatcggatc ggccatctca
    25561 actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc tgcgaccggc
    25621 gtaacggcca cggcaaggtg tttcatatca ccgttcactg ccaccagcag cgttttgtag
    25681 acctgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt ggtttcagcc
    25741 ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt ttttttcgag taatttaact
    25801 gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca tacgcctgat
    25861 ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt gagattgagc
    25921 gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa tgaaattcct
    25981 ctttgacggg ccaatagcga tattggccat ttttttagcg caacatttgc ggcaaattcc
    26041 cttctccata caggtgtagt gcaccgaccg cgaccacata tcgccccggc ggcatggcgc
    26101 gtaatttatc ccgccaggcg agatttcgct gatgcatcag cacatcgtac agcgactgac
    26161 tgaacgtatt gggcagcgtt atatcattat tttgcggcgg tgcattcagc caccagctca
    26221 tcatttgttg cagcaaccgt gcgttggtat gccagtgggt cagcgtatcg tccagcagcg
    26281 ccagtccttt gtcagggagc tggagcaaca tggcaatctg gttttcagcc ccttccagtt
    26341 caatcacggg tttatgttgt tgcttcgccg cctgcaatag ctggtaatcg ataccgtatt
    26401 ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag aaccatcgcg atttgccaca
    26461 gcggttgggt agaaaagagt gagggagaaa tgcccatctc ctggctaatg tgctgcaggt
    26521 tttggagttg ctcctcgcta atgcgctctt ccagcgcctc gcaggcaggc aaattagcaa
    26581 aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg tttttgagct
    26641 ttttgagcaa acgggtgggc aggggagcca tatcgtggct acccatatga atactgccaa
    26701 tcagatgaaa atggcgattg ccggggaggg tgatatcgat ggcaggccag gtgtaatgat
    26761 taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata cgacctccct
    26821 ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca aatggagttg gggacttgtt
    26881 taatgtgttt gtatgattca gtatgttctt gcatcgctat tcacagggaa gcaacagtta
    26941 aaaaccatga aacaggcaac aagaaaaccg acgacacccg gagatattct tctctatgaa
    27001 tatctggaac cgctcgattt gaaaatcaat gagttagcag agttgctgca tgttcatcgt
    27061 aatcgcgtca gtgcactgat caataacaat cgtaaactca ccactgagat ggcattccgt
    27121 ctggcgaaag tttttgatac cacagtcgat tttaggctaa acctccaggc ggcagttgat
    27181 ctttgggaag ttgaaaacaa catgcgcacc caggaagaat tgggacggat tgaaacagtg
    27241 gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtaagg ccaaaacatc
    27301 gcttcatctc gtaggccgga taaggcgttc acgccgcatc cggcatccga gcaccattgc
    27361 ctgatgcgac gcttacgcgt cttatcaggc ctacaaacct gtgcctgaac cgtaggccgg
    27421 ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg ccaaatgcgc cgccctaaag
    27481 cagcgcatcc gcaatgacgt acttattcct tcggtttaaa ccgcagcaac cggttggcgt
    27541 tacttactac ggtaattgac gagagcgcca ttgccgctcc ggcaactacc gggttaagca
    27601 gtgttccagt aaacggccac aaaataccgg cggcgaccgg aataccgatg ctgttgtaga
    27661 taaaggcacc gagcaggttc tgcttcatgt tgcgcagcgt tgcgcgggaa atcgcgagcg
    27721 catccgcaac gcccatcagg ctatggcgca tcagggtaat cgccgcggtt tcaatggcaa
    27781 catcactgcc gccacccatc gcaatgccga catccgcctg ggccagcgct ggcgcgtcgt
    27841 taatgccgtc gcccaccatt gccacctgac gtccttcact ttgcagacgt ttgatcgctt
    27901 cggctttacc gtccggcagc actccggcaa tcacttcatc aattccagct tctttggcga
    27961 tcgcattggc ggtggttggg ttatccccgg tcaacatcac cagacgatat cccgctttat
    28021 gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg atcgcgtaca gccagcaggg
    28081 ctactgcttt cccgtcaatc gccagcagca caggcgttgc tccttgcgat gcctgagctg
    28141 aaatatcggc ttcaatcgct ttagtatcga cttgctgatc atttaacaac gcctgattac
    28201 ccagcaataa cgcatgacct tctgcttcac cgctcacgcc cagcccgcgc aatgtgcgga
    28261 aaccgttgac ctgcggtagc tgcatatcgc ctgctttatc aaggatcgct cgtgccaatg
    28321 gatggctgga accttgctcc agcgccgccg ccagacgcag cgcctgcgct tcgtcaacat
    28381 cagcaaatgt tttcactgcg acaacctgcg gcttcccttc ggtcagcgtc ccggttttat
    28441 cgaacacgac ggtatcgagt gtactggcgc gttgcagcgc gtcggcgtcc cgcaccagca
    28501 cgccaaactc agccgcccgc ccgacgccgg aaataatcga catcggcgtc gccagtccca
    28561 gcgcacacgg gcaggcgata atcagcaccg tagtggcaat caccagggta tagacaatct
    28621 gcggtgccgg accaaagaaa taccagattg ccgcactgac aagcgcaata accactacta
    28681 ccggcacaaa tatggctgag attttatccg ccagctgacc gatttctggc ttgctgctct
    28741 gggcctggcg caccatgcga atgatccttg atagcgtggt atggctgcca accgcactgg
    28801 cacgaaacag cacactgccg tcctgtacca ctgtcccggc atggacgcta tcgccttcgc
    28861 ctttttgctg cgggattggt tcgcccgtca gcatcgcttc atccagccat gcttcgccct
    28921 gggtaatctc gccatctacc ggcacgcgat cgccggtcgt caggcgcagc aacatacctg
    28981 gctgcacttc tgccagaggc acgctttttt caccttcgtc agtaaccagg cgcgccgtcg
    29041 gtggggttaa atcgagtaat ttctccagtg ccttagaaga acgctgacgt gcgcgtgctt
    29101 ccagcatatg gccgagattg atcagaccga taatcatcgc actggcttca tagtaaagat
    29161 gtcgcgcttc catcgggaac cactgcggcc acaggttgac gctcatcgaa tagagccacg
    29221 ccacgcccgt tcccagcgcc accagcgtat ccatcgtcgc tgcaccgttc agcaggcttt
    29281 tccatgcact gcggtaaaaa tggccgctgg cgaaaaccat caccgccagg gttatcaggc
    29341 cgataaccaa ccacaggctg cggttgtcgg cggtgactat catgttatcg ccgatcatcc
    29401 cccagaccat caccgggata cccaccgcca gtgcgacaat tgcctgccag cggaagcgct
    29461 tcatcgtagc gacggcggtt tcttgctggc gttcgcggcg tttcgcgtca tcttcaatcg
    29521 cttccgcgcc gtagcccgct ttttccaccg cctgcactaa atcttgtggg gaggcactgc
    29581 ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc ctgagtgacg cccggtacgc
    29641 tttgcagcgc attttgtacg cgggtgacac agctggcgca gctcatgccg ctcagcagca
    29701 actgctggct gtcatcgtca tcggcggtcg ctgccggaag cgcctcagaa accgctgtca
    29761 gtgcttccga cgggattgat gactccgcca gcggtttagc ctttgggtgg cttacagatg
    29821 cgtcataacc cgcttgtttg atggtttcga tcagctgttc tgcactggca gtcccggtaa
    29881 cgtgcgcttc agtgatagac acatccgctt gttccacatc cgggcgctgt tcaagacttt
    29941 ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc caggttcagg tcgatagttt
    30001 gtgacataaa acactccttt aagacagttt tgactggctg tgataaaggt taaaccttcc
    30061 agcaagggga aggtcaagaa attaataaac caggcgggta aaagtccgta aagattaaaa
    30121 aatcggctcg atttgcatca ggattagaca tttatttctt tgttttcctg tagttaagtt
    30181 gcgggtgcta agttaaatca ggatgcctga aaatcggcac tggggtgagg aattacctcc
    30241 cgcatctata aaaaggagtt aacaaaagat gttagatgca aacaaattac agcaggcagt
    30301 ggatcaggct tacacccaat ttcactcact taacggcgga caaaatgccg attacattcc
    30361 ctttctggcg aatgtaccag gtcaactggc ggcagtggct atcgtgacct gcgatggcaa
    30421 cgtctatagt gcgggtgaca gtgattaccg ctttgcactg gaatccatct ctaaagtctg
    30481 tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg gtacaggaca aaattggcgc
    30541 tgacccgacc ggattgccct ttaactcagt tatcgcctta gagttgcatg gcggcaaacc
    30601 gctttcgcca ctggtaaatg ctggcgctat tgccaccacc agcctgatta acgctgaaaa
    30661 cgccgaacaa cgctggcagc gaattttaca tatccaacag caactggctg gtgagcaggt
    30721 agcgctctct gacgaagtca accagtcgga acaaacaacc aacttccata atcgggccat
    30781 tgcctggctg ctgtactccg ccggatatct ctattgtgat gcaatggaag cctgtgacgt
    30841 gtacacccgt cagtgctcta cgctcatcaa tactgttgaa ctggcaacgc ttggcgcgac
    30901 gctggcggcg ggtggtgtga atccgttgac gcataaacgc gttcttcagg ccgacaacgt
    30961 gccgtacatt ctggccgaaa tgatgatgga agggctgtat ggtcgctccg gtgactgggc
    31021 gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc ggtattctgg cggtcgtccc
    31081 tggcgtgatg ggaattgccg cgttctcacc accgctggac gaagaaggca atagtgttcg
    31141 cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc tataacgtgt ttaagggctg
    31201 atcatgatga acacggaagg taataacggt aacaaacccc tcggtctatg gaacgtcgtt
    31261 tccatcggta ttggggcaat ggtgggggcg gggatcttcg cgctgctggg gcaggctgcg
    31321 ttgctaatgg aagcctcgac ctggggcgcc tttgcttttg gcggtattgt ggctatgttt
    31381 tccggttatg cctatgcgcg tctgggggcg agctatccca gcaatggcgg tattatcgac
    31441 ttttttcgtc gcggattagg caacggcgtc ttttcgctag cgctctcgtt gctatacctg
    31501 ttgacgctgg cggtgagcat cgccatggtt gcccgtgctt ttggcgctta tgccgtgcag
    31561 tttttgcatg aaggcagcca ggaggagcac cttattttgc tctacgcgtt ggggatcatt
    31621 gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg tagggcggct ggaagtgatc
    31681 ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta ttgccggtgt ctggtcgctg
    31741 caaccggcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt ctcctgtatt
    31801 gggataacct tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc ggataaagtg
    31861 aaagatccgc aggtcattat gccacgggcg tttctggtgg cgattggcgt taccacgttg
    31921 ctttatatct cgctggcgct ggttttgctt agcgatgtat cggcattaga gttagaaaaa
    31981 tatgctgata ccgccgtagc gcaggctgct tctccgctgc tcgggcatgt gggttatgtg
    32041 atcgtcgtca tcggcgcttt actggcgacg gcttcaggca ttaacgcgaa cctgttcgcc
    32101 gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct aatgaataaa
    32161 tccctgtggc ggcagagtac ctggggcaat atcatcgtcg tggtgttgat aatgctgatg
    32221 acggcggcgc taaatttaga ctcactcgcc agcgttgcca gcgccacctt tttgatctgc
    32281 tatctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc ctcgttgccg
    32341 attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg ttggctttat ctacagcctg
    32401 tggtcccagg gcagccgtgc gttgatatgg attattggtg caatcttact cagccttatt
    32461 gtggcaatgg tcatgaagcg taataaaacc gtataacacc tctctgtgcg cagtacttcc
    32521 tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt gaatatctgt
    32581 tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatca gcgatgtagc
    32641 aaaaattacc ggcctgacca gcaaagccat tcgcttctat gaagagaagg ggctggtgac
    32701 gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc tcaacgaact
    32761 gaccttactg cgccaggcac ggcaggtggg ctttaacctg gaagagagcg gcgagctggt
    32821 gaatctgttt aacgacccgc agcggcacag cgccgacgtc aaacggcgca cgctggagaa
    32881 ggtggcggag atcgaacgac acattgagga gctgcaatcc atgcgcgacc agctgctggc
    32941 actggcgaat gcctgccctg gcgatgacag cgccgactgc ccgattatcg aaaatctctc
    33001 cggctgctgt catcatcggg cagggtgatt aagacgagac ggctcggata tgtagggtta
    33061 tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg ctggcgctga
    33121 caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc ggagattcca
    33181 gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgacta tcgctgtgct
    33241 tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca
    33301 ggatggcaaa cattacccct tgccactccc aacccagcgg caccagccag accaccaggc
    33361 cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc agcatctcgg
    33421 ctgccagcag caacccgccg agactgagcc agaaaatatg tggatgaacg accattaact
    33481 ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agctcggcaa tcccggcaat
    33541 cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt tactggagga
    33601 accgatctgc tgtaacgctt cggtgtattt ttgcgctacg aagtagttca ccgcctgaat
    33661 atcaccggag gcgatagctt cagacaccat tttggtggcg cgggcttctg cttcggcgga
    33721 acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat
    33781 ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc
    33841 aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag
    33901 ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc cccacgggtt
    33961 ggtggcttca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat
    34021 ttcgtcaagt tccattgaac ccaacacggt acggatgtta gtcatggtca ggttgatgat
    34081 cgccagctcc agattgctga cttcataggc agcgcgtggc gcgtcaatca cctgaataaa
    34141 acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat
    34201 atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac
    34261 cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta ccgtccattg
    34321 atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag
    34381 aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa
    34441 ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg
    34501 cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag
    34561 tcctttgctt cagctacaaa acgtaggata tctggcgggt gatacgaaga ttcttaataa
    34621 catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg
    34681 caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact
    34741 gtttgaaggt gaggatgtca gcacactaaa accagaaatc taccgccagc aagtctctta
    34801 ctgcgcccag acgccgacgc tgtttggcga tacggtatac gataatctga tctttccctg
    34861 gcagatccgt gaccagcagc ctgacccggc catttttctc gattttctcg aacgcttcgc
    34921 tttgccggac agcattttga cgaagaatat cgccgagcta tctggtggtg aaaaacaacg
    34981 catctcattg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg atgaaataac
    35041 cagtgcgctg gatgaaagta ataaacataa cgtcaatgag atgatccatc gttatgtgcg
    35101 cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga
    35161 taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct atgaactcgc
    35221 ataatattac taacgaatca ttagcactgg cattaatgct ggtggtggtg gcaatcttaa
    35281 ttagccataa agaaaaactg gcgctggaga aagatattct atggagcgtc gggcgagcga
    35341 taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca
    35401 gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac
    35461 gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg gtcggggcgg
    35521 gaattaccct ggcggtgctg attctctcag gggcgattga atttatcccg atgcaggtga
    35581 tccctgtcgc cgggatgatt gccggtaacg ccatggtagc ggtggggttg tgttacaaca
    35641 atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg agtcttggcg
    35701 cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc
    35761 cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg atgtccgggc
    35821 tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta
    35881 tgctgctctc aaccgccagc ttgtcgacca ttattgcctg ctatttaacc tatcgtaagt
    35941 tttataattc gcgccaccag ttggtggtga ctcaattgaa gaagaaatga tgcgatgccg
    36001 gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg aacaacggaa tatcaataca
    36061 acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag
    36121 cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca
    36181 gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctgatgc agttgcagcg
    36241 ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt tgcaactgtt
    36301 gaatttccgg cttatcagcc gcctgcttca gcagttcgat ttgcgctacc agcccctggt
    36361 agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac cgcttccgca tcttcagaac
    36421 ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ctccccgttc tgattcgaca
    36481 actgccaggc gtctttcagc aatggcaggg catcggtgta attgccttcc tgcatcagtt
    36541 gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc agcagggcgc
    36601 ggatcgcctc ttccggttgc ggcccctgga agccatctac cggttgcccg ttctggaaca
    36661 gatacacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc
    36721 agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcgaggctt tccaggatcg
    36781 gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg
    36841 tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta
    36901 cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg
    36961 cttcaactca cccctgcaat attttgtcca tcacgcgccc aggcagcagg cgctcgagca
    37021 ccattactgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa
    37081 aagcatggcg cactttgtcg accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc
    37141 cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg aagcgagtac
    37201 gaatgggacc gggttcgatc aggctgactt taattccgct gtggcgtagc tccatgcgca
    37261 gcgcgtctga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggcg
    37321 tggagattaa tcccatcacc gatgatgtca tcacaatacg cccttcaccg tgcggtaaca
    37381 tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct
    37441 gttccatctg cgcacggctg atggtggaaa gggggccata catgccgaat ccggcattgt
    37501 taaagatccc atacagacaa ttatcggtca gggcgatcac ctcgtctgct gcgcgattaa
    37561 cactttctgg tgaatccaga tcgatcaaca cgccggtaaa tcccatgttg ttcatacgct
    37621 caacatcatt cggttttcgg caacctgcca gcacatgaaa accctggcgt tttaattcga
    37681 gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa
    37741 ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaagg ctgcaactgc
    37801 ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc
    37861 tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag cggaacatca
    37921 aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag
    37981 tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc
    38041 aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa accatcattg
    38101 cgcccagttc aaccagcacc caacgcggct gatgctgttt gagcagagcc ggaaggcgcg
    38161 ccagcccttg ttgcgaggtg tcgccgctga tgctggcatt aactaccgac gttttactct
    38221 gccacttatc attcaacaag gcaggccagg ccgcgctggc agacattcga tacccggcgc
    38281 tcaggctatc acccagaatc aataacgtgt ccgctgcggc ggcacggaag gttaacagga
    38341 ccaggaacag gaagggcaaa tgccagcgga aaacattgtt gaagttcatc atcttaagaa
    38401 gtccgtcggt cagggggagc atgaactctc catcctcacc ggagttgagc tggttgtcaa
    38461 acgtggcgag accatcgcac tggtgggcga gtcgggatcg ggtaagtcaa ccttgctggc
    38521 gatcctcgcc gggcttgatg acggcagcag tggcgaagtg agtctggtgg gacaaccgct
    38581 acataatatg gacgaagaag cgcgggcaaa gttgcgcgcg aagcacgtcg gctttgtttt
    38641 tcagttattt atgttaattc ctacccttaa cgcgctggaa aacgtcgagc ttccggctct
    38701 gctgcgcggt gagagtagcg cggaaagtcg taacggggcg aaagcgttgc tcgaacagtt
    38761 agggctgggt aaacgtctgg atcatcttcc ggcacagctt tccggcggtg aacagcaacg
    38821 agtggcgctg gcacgagcct ttaatggtcg acctgatgtg ctgtttgccg acgaacccac
    38881 cggcaacctt gaccgccaga cgggcgataa aattgccgac ctgctgtttt ccctcaaccg
    38941 tgaacatggc accacgttga ttatggtgac ccacgacctg caactggcgg cacgctgcga
    39001 ccgctgctta cggctggtga acgggcagtt gcaggaggaa gcatgattgc acgttggttc
    39061 tggcgcgaat ggcgttcgcc gtcgctatta attgtctggc tggcgctaag cctggcggtg
    39121 gcctgcgtgc tggcgctggg caatatcagc gatcgcatgg agaagggctt aagccagcaa
    39181 agccgtgagt ttatggcggg cgatcgggcg ttgcgcagtt cacgcgaagt gccgcaagcg
    39241 tggctggagg aagcgcaaaa gcgcggcctg aaagtcggca agcagctgac tttcgccaca
    39301 atgacctttg caggcgacac accgcagctg gcgaacgtca aagcggtgga tgatatctac
    39361 ccgatgtatg gcggtctgca aactaatccc cctggcctga aaccgcaggc gggcagcgta
    39421 ttgctggccc cacgcctgat ggcactgctt aacctgaaaa cgggcgacac cattgacgtg
    39481 ggcgatgcca ccttgcggat tgccggagaa gtgattcagg aaccggattc cggttttaac
    39541 cccttccaga tggctccgcg tctgatgatg aatctggcgg atgtcgataa aaccggagcc
    39601 gtgcagccgg ggagtcgggt cacctggcgt tataaattcg gcggcaacga gaaccagctc
    39661 gacggctatg agaaatggtt gttacctcag cttaaacccg aacaacgctg gtacggtctg
    39721 gaacaggacg aaggcgcgct ggggcgatcg atggaacgct cgcaacagtt cctgctgctt
    39781 tcggcgcttc tgaccttgct gctggcagtg gcagcggtgg cggtagcgat gaatcattac
    39841 tgtcgcagtc gctacgatct ggtggcgatc ctcaaaacgc tgggggcagg gcgagcgcaa
    39901 ctgcgtaagc taatcgtcgg tcagtggttg atggtgctga cgctttcagc cgttaccggt
    39961 ggggccatag gcctgttgtt cgaaaacgtg ttgatggtgc tgctcaagcc cgttctgcct
    40021 gctgcactac cgccagccag cctctggccg tggctgtggg cgcttggcac catgacggtc
    40081 atctcgctgc tggtggggct acgaccatac cgcttgttgc tggcaacgca gcctttacgc
    40141 gtattacgta atgatgtggt cgcgaacgtc tggccgctga agttttatct gccgattgtc
    40201 agtgtggtgg ttgtgctgct gctcgccgga ttaatgggtg gcagcatgct gctttgggcg
    40261 gtgctggcgg gcgcggtagt actggctttg ctgtgcggtg tgctgggctg gatgctgctg
    40321 aatgtacttc gccgcatgac gctgaaatcg ctgcctctgc gcctggcggt tagccgcctg
    40381 ttacgtcagc cgtggtcaac gttaagccag ctttcggcat tttcgctctc ctttatgctg
    40441 ctggcactgc tgctggtgct gcgtggcgat ctgctcgacc gctggcagca gcagctaccg
    40501 ccagaaagcc cgaactactt tttaattaac atcgccacag aacaggtagc accgctaaaa
    40561 gcgttcctcg cggaacatca tatagtcccg gaatcatttt atccggtagt gcgggcgcgg
    40621 ctgacggcga ttaatgataa gccgacagaa ggtaatgaag atgaggcgct taatcgcgaa
    40681 ctcaatctta cctggcaaaa tacgcggccc gatcataatc cgattgtcgc cggtaactgg
    40741 ccgccaaaag ccgatgaagt gtcgatggaa gaggggctgg cgaaacgctt aaacgttgcc
    40801 ctcggtgata ccgtgacttt tatgggcgat acccaggagt tccgcgctaa agtgaccagc
    40861 ctgcgcaaag tggactggga aagtctgcgg cctaatttct attttatttt ccctgaaggg
    40921 gcattagacg ggcaaccgca gagctggctt accagtttcc gctgggagaa tggcaacggc
    40981 atgttgacgc aactcaaccg ccagttcccg accattagcc tgttagacat tggcgcgatt
    41041 ttaaaacagg tcggtcaggt gctggagcag gtaagtcggg cgctggaggt gatggtggtg
    41101 ctggtcaccg cctgcggtat gttgctgttg ctggcacagg tgcaggtggg aatgcgccag
    41161 cgtcatcagg agctggtggt atggcgcaca ctcggtgcgg ggaaaaaact gctgcgcacg
    41221 acattgtggt gtgatgttcg ccatgcttgg gtttgtttcc ggcctggtgg ccgcaattgg
    41281 tgcggaaacg gcactggcag tgttgcaggc gaaagtgttt gatttcccgt gggagccaga
    41341 ctggcgattg tggattgttc tgccgtgcag cggtgcgctg ctgctgtcgc ttttcggcgg
    41401 ctggctgggt gcgcgactgg ttaagggtaa ggcgctgttc aggcagtttg tggggtgatg
    41461 acaggtgtta gattgcctga tgcgctacgc ttatcaggcc tacgaagagc gtgcaatata
    41521 ttgattttgc aagattgtgt aggccggata aggcgtttac gccgcatctg gcatgaacag
    41581 tgtgcatttt ggcaataaat atgaaaccaa ctgtcatgtc ggttttttta aaggccgaag
    41641 gaggtaaacc ttcggcctta gctacgtttc ctcagttatt caataaactg acggatcagt
    41701 tccagcagcg cgattagcgc cttaagaacg atgagaactt tatcgagaaa agttttcatc
    41761 cgttgctccc ttaagggaga aagacaatca cagcttaccc ttacgttgcc tataactatg
    41821 attttcttca tgtgctagat gaggactgtc tctctccaaa ttagagcacc agaatcagca
    41881 ccacctgtat cctgaccagg acttaaaagt acttttccgc actgcggtaa tcccttgagg
    41941 cagtatcact actagcgtcg ggattgtatc gccaacatag ctgtttgaat atgtaggcat
    42001 cctgacttat aattcacttg ctagatgagg actgtttaat ttctgcccgg aaccaccaag
    42061 tcttgggcgg aaaaaataaa aaaaccgact gtcatgtcgg tttttttata tctgtattct
    42121 ggtgcaacca ttttgttctc acgacaatcg ccgtttcaga ataccgccca tcactgcgcc
    42181 ttaacccata ccgccacttc cgcccactca ccgcgaaaga caactttttc cgcttttttc
    42241 tcaagctgat agcgatacat tgggtcgtaa tattctttaa gtaacggcac cagccaggcc
    42301 agatgaccgt cggtgctgcc ggtggtgagt tgcgttgtca gcgccgtatc cagctgggca
    42361 gcgagttcgt tatagcgctg taaccccagc cgacgcttaa tcgccgaaag tccgtgatgc
    42421 aggtattcgc aatattcctg ccagccctgt tcgtcgccat aagcgtgggt aaaatcatga
    42481 tgcatacgca agaaatactc ttcattcagg cgttcaagac ggatctcaaa cggatcttct
    42541 accaccgcaa tcgccgcctg agtcattcgc tcgcgcagac attccggcag gtgattcgaa
    42601 ccgatcatcc ggctttcatc ttccagcacc cacaggcgca aatcctgacg ggcgtcggtt
    42661 tttagcattt cggcagccag caggttttca aaactcgcct ggctaagttg tggttgtaac
    42721 gtgcgaccaa acgccgaacc gcgatgacgc gccagccctt ccagatcaac accgtttggc
    42781 tgttgttgca ctaatagcgt tttaccgctg ccggtacaac cgccaatcag cactatcggt
    42841 ttttgtgcca gttcaatggt cgcctgaatc gcggtctggc gcagtgcctt ataaccgcct
    42901 tccaccagcg gataatcaat ccccgcttca tgcagccagc gttgcacaat atgtgaacgt
    42961 tgaccgccac gggcgcagca gagaatacct tgcggatttt gcaggcacgc tgcccgccag
    43021 gcgtacatac gctgctgacg aatttcaccc gccaccagtt tatgccccag cgccagcgct
    43081 gcgtctgagc cttgctgttt atagcaggtg ccaacggagg cgcgttcatc gttatccatt
    43141 aacggcagat tgatagcggc gggcattgcg ccttgctcaa actcgatagg ggcgcgaaca
    43201 tcaataatgg gcgtatcagc aatcagcagg gcacgatagt cctgttccgt gtgtctctct
    43261 tgcatagtta aaagtgaacc tcaaatcagc ttgcgcgcta ttttacgcgc caacgcgcaa
    43321 ggaaacttga tttttaactg cgtggggtgc cgaaaatttc taaaaatccg ctgatttccg
    43381 gcctgtgttg ggtaaacaag gtcacaatat cttctaccgc tttgcagctg ccaaatttgc
    43441 gccatgccag actcaatggc gaaggagggc gcatcgttgg gattacccgg ctgaccagtt
    43501 gttgattatc gatcattgac tggcaaagtg attttggcaa aaaaccaatg ccaacacccg
    43561 ccagatgggc ggcgattttc gtttccatat cgggaacaat aatctctttt tgccccggca
    43621 atcgccaggc gacgcgtttg gttaaggtgc cggcgctgtc ttcaatattg accgccggaa
    43681 agcgacgcaa ctgcgcttct gttagcggct cttcaacgtt cgccagcgga tgatccgccg
    43741 ccatgacaaa tcgccattgc accgatccta agggatcaag actaaaggta tttgccagcg
    43801 cctcagttcc cgtgaccccg atagccagcg aaaaaccttc gtacaatagc gagtcccaga
    43861 cgcccatata gatttgtcgg gagatgtgaa actgggtaaa ggggtaacgc tcattcagcc
    43921 acgccagcaa ctgggcgacg gcctgggggt tatagagcag gttgttgatg acaatattca
    43981 cctggcgttc cacgccatca ttcacctgtt gcagctcgct gggcatactt tccagccagc
    44041 tcagccagtc tctggcctgg gaaagtagat gctcgccagc cgctgtcaac gttacgctac
    44101 gagtcgtacg gaaaaacagc gctactccgg tattctcttc cagaagttta atgcgataac
    44161 tgatcgtcgc cgtggtttta cataatcgtt ctgccgcttt tgaaaaactt cctgtttcag
    44221 caaccgcaat gaaagtccgc aaggtttctg gatcgaacat cttcaggtat ccccttttaa
    44281 atccgcaagt tgcgtgattt tcttatcctc tgatttatca gtatttttac atgataatcc
    44341 tgttcaattt gtggactaaa tctagttttg gaaaaacatt ccaacttttg tattaatgtt
    44401 gttctcttaa ggttttagat tgcctgttat tgaaaccaag ctgaccggtc ggcggtggtt
    44461 gaacggaatt atgttacaag gacaaaaaga tgaaacttca ggtattaccg ttaagtcagg
    44521 aagcctttag tgcttatggc gacgtaatcg aaacgcagca acgggatttt ttccatatta
    44581 acaatggcct ggtggagcgt taccacgatt tggcgctggt tgagattctt gagcaagacc
    44641 gtacgcttat cagcattaac cgcgcgcaac cggcgaatct gccgctgacc attcacgaac
    44701 tcgaacgtca tccgctgggc attcaggcct ttatcccaat gaaaggtgaa gtgtttgtgg
    44761 tggtcgtggc gttaggcgac gacaaaccag acctgtcaac gctgcgggcg tttatcacca
    44821 acggtgaaca gggggtgaat taccatcgta acgtctggca tcacccactt ttcgcctggc
    44881 agcgcgtcac cgattttctg accatcgatc gcggcggcag tgacaactgt gatgttgaaa
    44941 gtattcctga acaggaactc tgttttgcgt gacgcctgca accgacttgc ataagataaa
    45001 ctaattgttc attgtttatg ctcacttgta ggtcggagtt aacgtaggta tgacggaagt
    45061 tagacggcgc ggcaggccag gacaggcgga gcctgtggca cagaagggcg cacaggcgtt
    45121 agagcgggga attgcgattc tgcaatatct ggaaaaaagt gggggaagtt cgtcggttag
    45181 cgatatttct ctcaatctgg atttgccgct ctccacgacc tttcgcttgc tgaaggtttt
    45241 acaagcagcg gattttgtct atcaggacag tcaattaggc tggtggcata taggattagg
    45301 tgtctttaac gtcggtgcgg cgtacatcca taaccgcgat gtcctctccg tcgccgggcc
    45361 gtttatgcgc cgcctgatgt tactttccgg cgaaacggtc aatgtcgcga tccgtaacgg
    45421 caatgaagcg gtattaattg gtcagttaga gtgtaaatcg atggtcagga tgtgtgcgcc
    45481 actgggcagt cgtctgccgc tgcatgcttc cggtgcgggc aaagcgctgc tttatccgct
    45541 ggcggaagag gagttgatga gcatcattct gcaaaccggt ttgcagcagt ttacgccaac
    45601 tacgcttgtg gatatgccca ccttgctgaa ggacctggaa caagcgcgtg aactgggcta
    45661 taccgtagat aaagaagagc atgttgtagg tctgaattgc atagcttcag caatttacga
    45721 tgatgtcggt agagttgttg ccgctatctc catctccggg ccttcatcaa gactgacaga
    45781 agatcgtttt gtcagtcagg gtgagctggt cagagacacc gcccgcgata tcagcacggc
    45841 gttgggactg aaagcacatc catagtgtct gtcgcatccc gctctgcgga gcgggttttt
    45901 ttgacaaaat ttgaaagttg gaaaattttt ccaactaata gaggtaggaa taaaatggca
    45961 aaaatgagag ccgttgacgc ggcaatgtat gtgctggaga aagaaggtat caccaccgcc
    46021 ttcggcgttc cgggagctgc aattaatccg ttctactcag cgatgcgtaa gcacggcggt
    46081 attcgtcaca ttctggcgcg tcatgtggaa ggtgtttcgc acatggcgga aggttatacc
    46141 cgcgcaacgg cagggaatat cggcgtatgt ctggggactt ccggtcctgc gggcacggac
    46201 atgatcaccg cgctctattc cgcttctgct gattccattc ctattctgtg cattaccggc
    46261 caggcaccgc gcgcccgtct gcataaagaa gattttcagg ccgtagatat tgaagcaatt
    46321 gctaaaccgg tcagcaaaat ggcggttaca gttcgtgaag cggcgctggt gcctcgcgtg
    46381 ctgcaacagg catttcacct gatgcgttct ggtcgtccgg gtccggtact ggtggattta
    46441 ccgttcgacg ttcaggttgc ggaaatcgag tttgatcctg acatgtacga accgctgccg
    46501 gtctacaaac ctgctgccag ccgtatgcag atcgaaaaag ctgtagaaat gttaatccag
    46561 gccgaacgtc cggtgattgt tgccgggggc ggggtaatca atgctgacgc agctgcactg
    46621 ttacaacagt ttgctgaact gaccagcgtt ccggtgatcc caacgctgat gggctggggc
    46681 tgtatcccgg acgatcatga actgatggcc gggatggtcg gtctgcaaac cgcgcatcgt
    46741 tacggtaacg caacgttgct ggcgtccgac atggtgtttg gtatcggtaa ccgttttgct
    46801 aaccgtcata ccggttcggt agagaaatac accgaagggc gcaaaatcgt tcatattgat
    46861 attgagccga cgcaaattgg tcgcgtgctg tgtccggatc tcggcattgt ctctgatgct
    46921 aaagcggcgc tgacactgct ggttgaagtg gcgcaggaga tgcaaaaagc gggtcgtctg
    46981 ccgtgtcgta aagaatgggt cgccgactgc cagcagcgta aacgcacttt gctgcgcaaa
    47041 acccacttcg acaacgtgcc ggtgaaaccg cagcgcgtgt atgaagagat gaacaaagcc
    47101 tttggtcgcg atgtttgtta tgtcaccact attggtctgt cacaaatcgc tgcggcacaa
    47161 atgctgcatg tctttaaaga ccgccactgg atcaactgtg gtcaggctgg tccgttaggc
    47221 tggacgattc cggcggcgct ggggggttgt gccgctgatc cgaaacgcaa tgtggtggcg
    47281 atttctggcg actttgactt ccagttcctg attgaagagt tagccgttgg cgcgcagttc
    47341 aacattccgt acatccatgt gctggtgaat aacgcctatc tcggcctgat tcgtcagtca
    47401 caacgcgctt ttgacatgga ctactgcgtg caactcgctt tcgagaatat caactccagt
    47461 gaagtgaatg gctacggtgt tgaccacgta aaagtagcgg aaggtttagg ttgtaaagct
    47521 attcgggtct tcaaaccgga agatattgcg ccagcctttg aacaggcgaa agccttaatg
    47581 gcgcaatatc gggtaccggt agtcgtggaa gttattctcg agcgtgtgac caatatttcg
    47641 atgggcagcg aactggataa cgtcatggaa tttgaagata tcgccgataa cgcagcggat
    47701 gcaccgactg aaacctgctt catgcactat gaataaggga gataaatgat gttacgtttc
    47761 tctgctaatt tatcgatgtt atttggtgaa tatgattttc tcgcccgttt tgagaaagct
    47821 gcgcagtgtg gttttcgcgg cgttgaattt atgtttcctt atgactacga cattgaagaa
    47881 ttaaaacagg tactggcgag taataaactc gaacatacgc tgcacaattt accggcaggt
    47941 gactgggcgg cgggcgagcg cggtattgcc tgtattcctg gccgtgaaga agagtttcgg
    48001 gatggcgtag cagcagcgat t
//
LOCUS       DAZPEU010000011        46473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPEU010000011 DAZPEU010000000
VERSION     DAZPEU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.011"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(26..1885)
                     /gene="gss"
                     /locus_tag="ACHU2L_000531"
     CDS             complement(26..1885)
                     /gene="gss"
                     /locus_tag="ACHU2L_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR1355524.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2090..2956
                     /gene="yghU"
                     /locus_tag="ACHU2L_000532"
     CDS             2090..2956
                     /gene="yghU"
                     /locus_tag="ACHU2L_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR1355525.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2985..3066)
                     /locus_tag="ACHU2L_000533"
                     /pseudo
     CDS             complement(2985..3066)
                     /locus_tag="ACHU2L_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3079..3327)
                     /gene="hybG"
                     /locus_tag="ACHU2L_000534"
     CDS             complement(3079..3327)
                     /gene="hybG"
                     /locus_tag="ACHU2L_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR1355526.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3461..3802)
                     /gene="hypA"
                     /locus_tag="ACHU2L_000535"
     CDS             complement(3461..3802)
                     /gene="hypA"
                     /locus_tag="ACHU2L_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1355527.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3795..4283)
                     /gene="hybE"
                     /locus_tag="ACHU2L_000536"
     CDS             complement(3795..4283)
                     /gene="hybE"
                     /locus_tag="ACHU2L_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR1355528.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(4276..4770)
                     /locus_tag="ACHU2L_000537"
     CDS             complement(4276..4770)
                     /locus_tag="ACHU2L_000537"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR1355529.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4770..6473)
                     /gene="hybC"
                     /locus_tag="ACHU2L_000538"
     CDS             complement(4770..6473)
                     /gene="hybC"
                     /locus_tag="ACHU2L_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR1355530.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6470..7648)
                     /gene="hybB"
                     /locus_tag="ACHU2L_000539"
     CDS             complement(6470..7648)
                     /gene="hybB"
                     /locus_tag="ACHU2L_000539"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR1355531.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7638..8624)
                     /gene="hybA"
                     /locus_tag="ACHU2L_000540"
     CDS             complement(7638..8624)
                     /gene="hybA"
                     /locus_tag="ACHU2L_000540"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR1355532.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8627..9745)
                     /gene="hybO"
                     /locus_tag="ACHU2L_000541"
     CDS             complement(8627..9745)
                     /gene="hybO"
                     /locus_tag="ACHU2L_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR1355533.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9934..10221)
                     /gene="yghW"
                     /locus_tag="ACHU2L_000542"
     CDS             complement(9934..10221)
                     /gene="yghW"
                     /locus_tag="ACHU2L_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR1355534.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10340..11227)
                     /gene="yghX"
                     /locus_tag="ACHU2L_000543"
     CDS             complement(10340..11227)
                     /gene="yghX"
                     /locus_tag="ACHU2L_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR1355535.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11384..12424
                     /gene="gpr"
                     /locus_tag="ACHU2L_000544"
     CDS             11384..12424
                     /gene="gpr"
                     /locus_tag="ACHU2L_000544"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR1355536.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12464..12958)
                     /locus_tag="ACHU2L_000545"
     CDS             complement(12464..12958)
                     /locus_tag="ACHU2L_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR1355537.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13149..14033
                     /gene="yghA"
                     /locus_tag="ACHU2L_000546"
     CDS             13149..14033
                     /gene="yghA"
                     /locus_tag="ACHU2L_000546"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1355538.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14305..14730)
                     /gene="exbD"
                     /locus_tag="ACHU2L_000547"
     CDS             complement(14305..14730)
                     /gene="exbD"
                     /locus_tag="ACHU2L_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR1355539.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14737..15471)
                     /gene="exbB"
                     /locus_tag="ACHU2L_000548"
     CDS             complement(14737..15471)
                     /gene="exbB"
                     /locus_tag="ACHU2L_000548"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1355540.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15723..16910
                     /gene="metC"
                     /locus_tag="ACHU2L_000549"
     CDS             15723..16910
                     /gene="metC"
                     /locus_tag="ACHU2L_000549"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR1355541.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17050..17709
                     /gene="yghB"
                     /locus_tag="ACHU2L_000550"
     CDS             17050..17709
                     /gene="yghB"
                     /locus_tag="ACHU2L_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR1355542.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17749..18648)
                     /gene="yqhC"
                     /locus_tag="ACHU2L_000551"
     CDS             complement(17749..18648)
                     /gene="yqhC"
                     /locus_tag="ACHU2L_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR1355543.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18842..20005
                     /gene="yqhD"
                     /locus_tag="ACHU2L_000552"
     CDS             18842..20005
                     /gene="yqhD"
                     /locus_tag="ACHU2L_000552"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR1355544.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20110..20937
                     /gene="dkgA"
                     /locus_tag="ACHU2L_000553"
     CDS             20110..20937
                     /gene="dkgA"
                     /locus_tag="ACHU2L_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR1355545.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            21137..22063
                     /locus_tag="ACHU2L_000554"
     CDS             21137..22063
                     /locus_tag="ACHU2L_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR1355546.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22114..22371
                     /gene="yqhH"
                     /locus_tag="ACHU2L_000555"
     CDS             22114..22371
                     /gene="yqhH"
                     /locus_tag="ACHU2L_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR1355547.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22414..24633)
                     /locus_tag="ACHU2L_000556"
     CDS             complement(22414..24633)
                     /locus_tag="ACHU2L_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR1355548.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24744..26156)
                     /gene="ftsP"
                     /locus_tag="ACHU2L_000557"
     CDS             complement(24744..26156)
                     /gene="ftsP"
                     /locus_tag="ACHU2L_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR1355549.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26231..26968)
                     /gene="plsC"
                     /locus_tag="ACHU2L_000558"
                     /gene_synonym="parF"
     CDS             complement(26231..26968)
                     /gene="plsC"
                     /locus_tag="ACHU2L_000558"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR1355550.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27202..29460)
                     /gene="parC"
                     /locus_tag="ACHU2L_000559"
     CDS             complement(27202..29460)
                     /gene="parC"
                     /locus_tag="ACHU2L_000559"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR1355551.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30006..30488)
                     /locus_tag="ACHU2L_000560"
     CDS             complement(30006..30488)
                     /locus_tag="ACHU2L_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR1355552.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30541..30933)
                     /gene="ygiW"
                     /locus_tag="ACHU2L_000561"
     CDS             complement(30541..30933)
                     /gene="ygiW"
                     /locus_tag="ACHU2L_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR1355553.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31085..31744
                     /gene="qseB"
                     /locus_tag="ACHU2L_000562"
     CDS             31085..31744
                     /gene="qseB"
                     /locus_tag="ACHU2L_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR1355554.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31741..33015
                     /gene="qseC"
                     /locus_tag="ACHU2L_000563"
     CDS             31741..33015
                     /gene="qseC"
                     /locus_tag="ACHU2L_000563"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR1355555.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(33061..33393)
                     /locus_tag="ACHU2L_000564"
     CDS             complement(33061..33393)
                     /locus_tag="ACHU2L_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFR1355556.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            33712..34293
                     /gene="mdaB"
                     /locus_tag="ACHU2L_000565"
     CDS             33712..34293
                     /gene="mdaB"
                     /locus_tag="ACHU2L_000565"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR1355557.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34324..34638
                     /locus_tag="ACHU2L_000566"
     CDS             34324..34638
                     /locus_tag="ACHU2L_000566"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR1355558.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34686..36578)
                     /gene="parE"
                     /locus_tag="ACHU2L_000567"
     CDS             complement(34686..36578)
                     /gene="parE"
                     /locus_tag="ACHU2L_000567"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR1355559.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36607..37188)
                     /gene="yqiA"
                     /locus_tag="ACHU2L_000568"
     CDS             complement(36607..37188)
                     /gene="yqiA"
                     /locus_tag="ACHU2L_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR1355560.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37188..38015)
                     /gene="cpdA"
                     /locus_tag="ACHU2L_000569"
     CDS             complement(37188..38015)
                     /gene="cpdA"
                     /locus_tag="ACHU2L_000569"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR1355561.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38040..38462)
                     /locus_tag="ACHU2L_000570"
     CDS             complement(38040..38462)
                     /locus_tag="ACHU2L_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR1355562.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38463..39092)
                     /gene="nudF"
                     /locus_tag="ACHU2L_000571"
     CDS             complement(38463..39092)
                     /gene="nudF"
                     /locus_tag="ACHU2L_000571"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR1355563.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39297..40778
                     /gene="tolC"
                     /locus_tag="ACHU2L_000572"
     CDS             39297..40778
                     /gene="tolC"
                     /locus_tag="ACHU2L_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR1355564.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40926..41597
                     /locus_tag="ACHU2L_000573"
     CDS             40926..41597
                     /locus_tag="ACHU2L_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR1355565.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41603..42763
                     /locus_tag="ACHU2L_000574"
     CDS             41603..42763
                     /locus_tag="ACHU2L_000574"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1355566.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(42801..43589)
                     /gene="ygiD"
                     /locus_tag="ACHU2L_000575"
     CDS             complement(42801..43589)
                     /gene="ygiD"
                     /locus_tag="ACHU2L_000575"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR1355567.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43732..44505
                     /gene="zupT"
                     /locus_tag="ACHU2L_000576"
     CDS             43732..44505
                     /gene="zupT"
                     /locus_tag="ACHU2L_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR1355568.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44563..44733)
                     /gene="yqiD"
                     /locus_tag="ACHU2L_000577"
     CDS             complement(44563..44733)
                     /gene="yqiD"
                     /locus_tag="ACHU2L_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR1355569.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44994..45647)
                     /gene="ribB"
                     /locus_tag="ACHU2L_000578"
     CDS             complement(44994..45647)
                     /gene="ribB"
                     /locus_tag="ACHU2L_000578"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR1355570.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45751..45899)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46021..46311
                     /gene="ubiK"
                     /locus_tag="ACHU2L_000579"
     CDS             46021..46311
                     /gene="ubiK"
                     /locus_tag="ACHU2L_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR1355571.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11553 a        12412 c        11581 g        10927 t
ORIGIN      
        1 cagtacgggg ttgctacaac atgtattact ttttcaccac aattaacggt tcaatatcac
       61 tctctttttt gatgaccagt gattcatcac cgcgcaaaca cgtcccaccg tagttgccgc
      121 caacggtgaa ggtacatacc tgaatgtatt taccgtccac tttcggcaaa caccacagtt
      181 gctgatagat gtttttctgc tcggcaaatt taccgctggt tttgtccagc acctcttcat
      241 gatggctgac caggtcgata ttgctaccac agcgaccggc gatcggtttc actgcgtaac
      301 ctgttttcac caattcatca ttaacagtga aatcggtatc cagcaggtaa cgatggtgcg
      361 ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc ggggatcacc gtccacagcg
      421 gctcaaagac cagcacttcc gggcgcagca atacgtcgat aagacgcact tcgttttgcg
      481 gatgaccggt acggattggc accgcagcaa actcacggtc gctaacttca cgaatctgat
      541 caaacgcggt ttcccacgcc caggttttcc acacgcagtt aaccagtcgc ccttccccat
      601 caatcagttg cccggcagca tcccagccca gttcatccag cccgcgcaag atacgcgttt
      661 caaagcccgc ctggtgcagc gcctgctcca taaactgcgc gtgatagttt tcctcgatat
      721 ctttgtcctg catgatatgg acaaacggac gtgcacgact gtgtttccag gcaccagcca
      781 gttcgttaat cagcccttcc gccggattga agccgttgcc tttatagccc tgctccgccc
      841 aacgttcgag gatcaagccc gcttcggtat gacaggaggc ggagtcggcg ttgtattcgt
      901 aaaccttcag gccacgctca tccatgcaga aatccatacg accagtgatc atatggtgac
      961 ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat tttcggaatg tcgaacagcg
     1021 ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg aagatacatc agatgcagct
     1081 cgttggtggc tttaattagc tcctgctcgg cactctcggt aatggtgtag taatgatacg
     1141 gatcctgatt gatcacctga ccgttggcct gcacataggc gttttgtagc ggatcttttt
     1201 catccagcca tttaccgtca aactggcctt tgttttccag acgcgctcca ctgattttca
     1261 gcagctcgcc tgcaatttcc ggctgcggta agctgtattc agtatcttcc gtctggatca
     1321 tccagcccag aatggtggtg tcatcaaaag tgtctttaag ggtatagcag ccgttttcga
     1381 ccaccatctc cagctcgcgc gtccactgtt gcccttgcgg caacggggaa tgaatcacgt
     1441 tctgttccgc aatacggact ttgttgccat gcaattgggt aatgatggcg acatggccag
     1501 tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc acccgcgacc ggcgcacgcg
     1561 gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc attaaccact tctcgcagga
     1621 agcgcagcga gaaaatctcc cacgccatac ccacgtcagt aaagaccaca ccgtaattca
     1681 gaaagagaaa acggcgagca aattcaacgc attgccactt gtggcccata tattcgtcgt
     1741 cgatatagct acggaatacg gcgtcatctt cgtattcctg cggatcgaga gaactgtaat
     1801 ctgaagagta gattgctacc ccacctgggg cgtagcccaa taatgtcccg aacggggcat
     1861 cctggctggt cgttcctttg ctcatcactt tacctttaac aatacaacct aagcagttgg
     1921 cgtaattgtt gtgctctgat tacctgctca gcgacattaa ccggacagag gtcagactaa
     1981 tcatacacct catcgcagcg gctggcgcaa agggttaaaa aattcacatt ctgtacagca
     2041 agtgacctgc tacactgctt caacactacc actcagaagg caactcacta tgacagacaa
     2101 tacttatcag cccgcgaaag tctggacgtg ggataaatcc gctggcggcg cgttcgccaa
     2161 tatcaatcgc ccgttttctg gtccgacgca tgaaaaaacg ctgcccgttg gcaaacatcc
     2221 attgcaactt tattcgctgg gaacgccgaa cggtcagaaa gtaacgatta tgcttgagga
     2281 gctactggcg ctgggcgtta ctggtgcaga gtacgacgcc tggctgattc gtattggcga
     2341 tggcgatcaa ttctccagcg gctttgtcga agtgaaccca aactcgaaga tcccggcgct
     2401 gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa tctggttcga tcctgcttta
     2461 tctggcggag aaatttggct acttcctgcc gcaggatctg gcaaaacgga ctgaaacgtt
     2521 gaactggctg ttctggttac agggtgccgc gccgttcctc ggcggtggtt ttggtcactt
     2581 ttaccattac gcgccggtga aaattgagta cgccatcaac cgctttacca tggaagccaa
     2641 gcgcctgctc gacgtgctgg ataagcaact ggcgcagcat aagtttgttg cgggcgatga
     2701 gtacaccatt gcggatatgg cgatttggcc gtggtttggc aacgtggtgc tcggcggcgt
     2761 gtatgatgcc gcagagtttc tggatgcggg aagttataaa catgtgcaac gctgggcgaa
     2821 agaagtgggc gaacgtccgg cagtgaaacg tgggcgtatt gttaaccgca ccaacgggcc
     2881 gctgaacgag cagttgcatg agcgccatga cgccagtgat tttgagacaa atacagaaga
     2941 taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc catccaggcc ggataaggcg
     3001 ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg acgctgccgc gtcttatcag
     3061 gcctacaaaa gctaatcatc acgcgctggt aatgtcgtaa tccatttggc gcagtgcgtc
     3121 taatgtggct ttggcttcat cttcgtcgat gatgctcatg gcaaatccga cgtgtaccag
     3181 cacccactgg cccagcagat cggcagggtt accttcgcaa atcagggcga tattcacgtc
     3241 gcgcttgata ccacatactt caacctgcgc aagctggtga atatcttcac cgacagccag
     3301 cacctggcct ggaacgccaa tacacattgt taactccggt tatttcgatt gccggacaaa
     3361 cgcgaaaaag aacctttagc gggtgttccc tctcacccta accctctccc cggaggggcg
     3421 aggggaccgt ccgtgcgctt tttaggcgtt gtcctcagtc ttattcaact tcaatacttt
     3481 tgacgatcag cgaatcgccg gtatcgacac gcaaccgctc gccgtgacag agcggacact
     3541 gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc ccagcaccag gcctgggcgg
     3601 gtttatagac gatatgtaaa tcgcaccctt gcgccaccgt tccgtggcag acaatttcaa
     3661 aactaaaacg gacggcgctc tcctcaacgc aggagagcgc gccaatttcc agccacacgg
     3721 cggtgacgcg cttaacatcg tgctgctccg cctgccgttg gataatttca acggcgctct
     3781 ggcaaagcga caactcatgc attttcgccc ctcctgcggc caaacagcag ggcgcgacgc
     3841 cctgcgtgtg gtacatccgg attggtgact ggcaacgaaa ggatcattcg tgcgcagtca
     3901 tccgtcaggc gttggccttc ttcaatcgac atgctgtgcg aaagcggcga catcagcgaa
     3961 caggagaggt attgcgaaac accgtccagt tcgccaacgg taaaggtcat ggtgccatac
     4021 ggcagttgca gaccaatttt ttcactcact ttgcgcagcg gccagagttg atccgggccg
     4081 gggaagataa ctgcactcag catccacggg gtgatcacac accccgtcca ctgaccttcg
     4141 aacagcgtaa aatcagaaac atacaccggc attgaaggat gcagaaaaga gagatcgtgc
     4201 atcgaacgcc gggcaatttc ttcaaacgct gcctgtactt gcgccttcgg ggaggtctgg
     4261 aaacctgcta tctcttcagt catgaatcgc ctcccgtggg atggcttcca cgcctgattc
     4321 gcgcagcgca gccagaacct gctcaagcgc aggttcaatc attgcttcaa ccgtcggcgt
     4381 taagccgatg tgtggctcca gcgattccgg gatcacgccg accagggtca gctttttcgg
     4441 aaactcaccg gtgaagcgca gggccgacaa tacgtcggcc aggccaagct gatgcggaga
     4501 gattttgttg gtaaacaacg ccggaacttc ttcatcccgc aggatcatca tcgttcccgg
     4561 agtgttcttt ttcgacacaa tggcatccgc aataatcaga tgatcgcgat ttgccatgtc
     4621 gccaagcagc tccattcccg ccgtgccgcc atcgaggatc tcaacataat ccggcagaat
     4681 gtatcgttgc tctaacgctt cgacaatccg cacgccgatg gcttcatcgg tcagcaaaat
     4741 attgccgacc cctaagacta aaatacgcat tacagaacct tcactgaaac cacttcgttg
     4801 ccgtcggcat ccactacgtg taccgcacag gccatgcacg ggtcgaagga gtgaatggta
     4861 cgcaccactt ccagcggttt attcggatcg gcaaccggtg tacccaccag cgactgctcg
     4921 taaggaccga cgtcatcatt gaagttacgc ggaccagagt tccaggttga tggaacaacc
     4981 gcctggtagt tgctgatgat accgtcttta ataaccatcc agtgagagag cataccgcgc
     5041 ggcgcttcga ggaagccaac acctttgaac tcacccgttg ccggaatgtt cggcttcaca
     5101 aaggtggtgt gatcgccttt gccgatattg gtgatcagtg cactgtattg gttttgcagg
     5161 atatcctgca attcacagca gtgaacggta cgaccaataa tacggcccag cgtggagtgc
     5221 agctgtgcca cttccagcgt gttgccagtc agtttctgat aaatcgcaac gatttcattc
     5281 agtttgttct gggtagattc gcgacctgcc gccagtttca ccagcatatt agccagcgga
     5341 cccacttcta ccgttttgcc gtagaaagtc ggtgatttca cccaggaata tttcccgtcg
     5401 tcagaccaac catcataagc cggaatggtg gtaccttccc acggcgcctg tggcgcttcg
     5461 tctttatacc aggagtgctt cgcgctttcc tgaatgcctt tgatcaggta ttcatcggaa
     5521 tgagaagtga tcggacgata cgaggacaga tccgcattct caatgtagcc tcccgggaac
     5581 aggaagctgc cgtttttgct gtcggtcggg aattccggca cgctcaggta gttcaccgca
     5641 cctttaccgc gcgtcagcca ttccgggtag aacgcggcga taaccgcggt atcaacctta
     5701 taaacctgct caacaaagtc gctcagtttg tcgatgaaag acttgatgta catcaggcgc
     5761 tcaaggttca acacgcccaa cccgtcgagg ttgattgggt tcgcgacacc acccacagcc
     5821 aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc
     5881 tggcactcca gcgcctgcag gtagtgcgct accgcaatca ggttcacttc cggcggcagt
     5941 ttcatcgccg ggtgacccca gtagccattg gcgaaaatac ccaactgacc gctggcaacc
     6001 agatctttga tcttgttctg aactttagtg aactcttccg gactgttcag atgccaggtc
     6061 gaaacgcctt tcagcatttc ggaggctttg gttgggtcag cttgcagtgc agaagtgatg
     6121 tccacccagt ccagcgccga aagctgatag aaatgaacga tatggtcatg cgtggtgtgc
     6181 gcagccagaa tgatgttacg gatgtattgc gcgttaaccg gaacgtcgat attcagcgca
     6241 ctttctgccg cacgaacgga agacagcgcg tgagtggtag tacatacgcc acagatacgt
     6301 tgcacaatca tccatgcatc gcgcggatcg cggtttttca cgatctcttc catgccgcgc
     6361 cacatggtac cggaagccca tgctttcgaa acgacgccat tttcgatttc gcaatcgatg
     6421 cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa ttctctggct catgctttgc
     6481 tcgcctcatg acgattatga tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa
     6541 tgagtacgat gtaagcgcaa atctcaatag ccacaaaacc aatagaaatc aacagttctt
     6601 cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa tgccaccaac gaataggtca
     6661 gacgccaggt tgcacaacct aacagtgcgc tcagtgctga caggaacagc atacgggaat
     6721 cattacgcag cttcgccaca cgcagaacga ccagcgggaa gagcatcagc aggacttcaa
     6781 tccagaacat cacggagtag aagtcaccgg caaacgctaa cgacagcttg tcgcgataga
     6841 taagctcgcc aaagcgcagc ccgatgaaaa tcgccagcaa cacactgatg gtgttggtca
     6901 gcttgacgaa caggctcttt tcatccggac cgttgccacg cagacccgcc tgcaccagtg
     6961 aaccttcaaa gatgacaatc gagaagccca tgatgaacgc cgtcagcagc gagaacagcg
     7021 gcaacatttc atagctctgc cagagcggat gcaccttgta gcccgccgag atcatcagcg
     7081 accccattga agactggtgc atggtcggca gcagcgcacc gagcgcgatg atgaagaaca
     7141 tcaccttgtt cagacgttgt agcgacacct tccagcccag acgttcaaac agtgccggag
     7201 caaactccag agccatcacg ccgatgtaga tggtcataca gaccgccgtc tcgaacagta
     7261 ccgagttcac gttgaagtga cccggaatgt agaagtacgg caggttccag tagcgaccca
     7321 cgtcgatagt gatcgacaag ccacccagtg agtaaccaaa cagactcgcc aacagcgccg
     7381 gacgcaccag cggatggtat tgcccacggt taaagacgta taccgcccac gccagcgccc
     7441 agccgccaca ggcaaagccg gtgccaatca gcaggtcaaa cgcgatccac acgccccacg
     7501 ggaagccgcc gttcaggtca gagacagagc ccagaccgaa caccagacgc ttcacaatca
     7561 ggagcataca gatgacgatt aacggtccaa aaatcatgac cggtttactg atgattttgc
     7621 cgcccagcgg ttgtggatca tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg
     7681 gtgttgcgac gaaccagcac ggttaagccc gccagcacag ccagtggtag catcatgcct
     7741 ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg tagaaagatc gtccagtttc
     7801 ggcaggtcga gattttcata aggcacaccc gtcagtacca gaacctgagt accgccgccc
     7861 tctttctcgc cgtacagatg cggataatat ttcggcaccg tatgcaggta agtgtcgcca
     7921 gatttcagcg tctgacgcgg atagtggtat tcgctgccag gcttcagcgc cagacgtttt
     7981 ttcgcctccg ccatcagctc ttcacgcgta ccgaaaatca ccgcgcccgc cgggcacact
     8041 tctacgcagc caggcagacc gcctttatcg agacgttcca cacctttctg gttgcacagc
     8101 tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt acttcggcac gttgtacgga
     8161 caggcgacca tgcagtaacg gcaaccggtg cacacgtctt tgtcgtaatg gacaatgccg
     8221 gttttcggat cttttttcag tgcagagacc gggcacacag agacacagtt cggatcgacg
     8281 cagtgcatac actgtttctt aatgtacgcg tagccgttct cctcctggtc tttgttgacc
     8341 cctgtgccgc tggtccacac ctgaatgatg ttattggtat acggcgacag tttgtcgttg
     8401 ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga agttgatatc ctgacacttg
     8461 gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt acaacatccc cagcgatccc
     8521 ggaattggcg ggcggttttc agcagccgca tgactgacag acggcagcgc gcccgtcagc
     8581 aatgccccgc aggaggctgc tttaataaaa ttacgtctgt tcacggttat tctccccgtg
     8641 agtcagcgtt atctttcttt tgctgacgac ccagttcacg caccgccatc acgctgacac
     8701 cggcaaccag cccaaccaca ccgccgagca aaccaatagc gcctgcagag acgttgccgc
     8761 cctctttagc gttaacatcc ggtttctgtg aacgcggagt ctgattttcg acgttggcaa
     8821 gctgatggat gcctttatgg aagccgatac ctttttcgtt acagccatag cacgggtgac
     8881 caatcgccac cggccacaca ccgccaacat cgcagaattg cagcgttgag cagttgccgt
     8941 aagtttctgg ccctttacag ccgaggtggt acaagcacca gccttcgcgg tggccttcat
     9001 caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt
     9061 gaatcagacg gccataggcg aaggtcggac ggtttttgtc atccagtttc ggcggtttgc
     9121 cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg cgggttcggc gggcagcccg
     9181 gaatattgat aacggttttg cctggcagaa cttcttgcaa gctgactgcg ccagttgggt
     9241 taactccagc tgcggcaaca ccgccccacg cggagcagga accaatagcg ataatggctg
     9301 ctgagccttc cgccgctttg cggatgtgat ccacaatcgg ctcaccggca accatgcaat
     9361 aaataccgtt atcttttaat gggatggaac catccaccac taacacatac tgccctttgt
     9421 acttctcgag agcgttatgt ttgttctctt cgacctgatg accgaaggcg gcggaaagca
     9481 cttcgtgata ttccagagag atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg
     9541 cacgaagcag agattccgta caaccggtgc actcctgcgc gccaatccag ataactggcg
     9601 gacgctgcgg gttagtaacc gattcggcca tctctgcagc ggctttgcta cttaacccca
     9661 tggtggcggc taatgctgca caaagcttca tgaaatcacg acggttaatg ccgtgagaat
     9721 ggatgagggt gttatctcca gtcatttata gttattccgt tgcgagaacc tggcatatat
     9781 tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc atttaccaca catttattat
     9841 ggttatcgat atcatgatac tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt
     9901 aattaattaa agcaagataa tacgtatgtt tgatcaattt tcgaggcgat aaccggccat
     9961 aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag aaatccatta ccatctcttt
    10021 atccagtcca tagcggcgcg tcagaatacc cgcttcccag gcgctaagct ggcgatctcc
    10081 ggtcttttcg tacatcctgt gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca
    10141 gaattttgtc acattaaccg cactgtcggc atgcgttgct tttaatcccg ccattaagcc
    10201 tttaccaaaa tggttattca tcgttcacct cattcattat atatcgccat atataacgat
    10261 attttaggca aagaacgact tttgcgcacg ctattttttc atacttgttt aagacgtaat
    10321 cccgtaagag ataaacctat caggagagat atttatcgaa ccatttcagt gtcctttgcc
    10381 aggcaagatc ggcggcagat ttgtcataac ggggcgtgga atcattatgg aatccgtgat
    10441 taacccccgg atagatataa gcctcataaa ccttattatt gtctttcaac gccgcctcgt
    10501 aaggaggcca gccctcgttg attcgggtgt ccagttccgc gaagtggagt agtaaaggcg
    10561 cttcaatctt cgccacatcg gcagtgggtg cctgacgacc ataaaacggc accgcgcagg
    10621 ccagttccgg atacgcgaca gccgccgcgt tcgatacgcc accgccatag caaaatccgg
    10681 taatacccac tttacctgtc gcttgcggat agcgttgcat aaactcaatt gcggcaaaga
    10741 aatcattcat cagtttggtt ggatcaacct gctgttgcag ctcacgacct ttatcatcat
    10801 ttcccggata acctccaacg gaacttaatc cgtcaggtgc cagggcgata taccccgcct
    10861 tcgccactcg ccgtgccaca tcttcgatat acggattcag tccacgattc tcatgcacca
    10921 cccccacggc tggcgttttg ccgctcattt ttgcgggttt caccagataa ccccgtacct
    10981 cgccgtgacc atttggcgaa ggatacgtga tgtactcagc aacaatctcc gggtcggtaa
    11041 actctacctg agtcgccagc gcataatttg gcttgagcaa atcaaacaac gctaatgccg
    11101 tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa ctcacgtttc gaaattttcc
    11161 cgtgaacgta atagtcgtag taatccaaca actcttgtgg gaaatctttg gcggttaaac
    11221 gcggcatcgt tgcattcctc aactggtgtt tttttaagca aagcataagc gcgtgttttt
    11281 gcccagtttt tcgtcactct gtgagccaga ctacgggata cgcgctggcg aatcgctaaa
    11341 ctagaaacat tgtttcgaaa ttgaacggtg gaaaggagag gtcatggtct ggttagcgaa
    11401 tcccgaacgt tacgggcaga tgcagtaccg ctattgcgga aaaagtggtt tacgcctgcc
    11461 cgcattatcg ctcggtttat ggcacaattt cggtcacgtt aacgcgctgg aatcacagcg
    11521 tgcaatcctg cgtaaagcgt ttgatttagg cattacgcac tttgatttag ccaacaatta
    11581 cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc ctgctgcgcg aggattttgc
    11641 tgcttatcgc gatgaactga ttatctctac caaggctggc tacgatatgt ggcccggccc
    11701 gtacggctct ggcggttcac gtaaatacct gctcgccagc ctcgaccaaa gcctgaagcg
    11761 tatggggctt gagtatgtcg atatctttta ctctcatcgc gtcgatgaaa atacgccgat
    11821 ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc ggtaaggcgc tgtatgtcgg
    11881 gatctcctct tactcgccag agcggacgca aaaaatggtc gagttgctgc acgagtggaa
    11941 aattccgctt ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag
    12001 cggcctgctg gataccctgc aaaataacgg cgtgggctgt attgccttta ctcctctggc
    12061 tcagggattg ctgaccggaa aatatctcaa cggcattccg caagattcac ggatgcatcg
    12121 tgaagggaat aaagttcgtg gtctgacgcc gaaaatgctt accgaagcca acctcaacag
    12181 cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt
    12241 aagctggttg ctgaaagatg atcgcgtgac gtcggtattg attggtgcca gccgcgcgga
    12301 gcaactagag gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc
    12361 gcagattgat cagcatatcg ccgatggcga gctgaatctg tggcaggcgt cgtccgataa
    12421 atgacctgtt aataacgggc gcgcgaaccg cgcccgaatc agatcagtga ttatgacgag
    12481 tcagtcggtc aagatagccc atcacaaatg cagaaagcac aaacgtcaga tggataatga
    12541 cgtaccacat cagtttgtta tccgggacat ttttcgcatc cataaagacg cgcagtaagt
    12601 gaatggaaga aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca
    12661 ttttccccag ccacttcagc ttctctttgt tctcggagat atccagctgt gagacgaaat
    12721 tctcataacc ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca
    12781 gcgacagcaa cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt
    12841 gaataatctc ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt
    12901 acacgggggc aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac
    12961 attcctgtcg tgtagttaaa acgggcaaca gtatatcgca attgagtaaa cagcacgcaa
    13021 cagcaaatca acatatacgt taaacctttg tcgcataatt ctgaggttta gggatcttcc
    13081 tggttccctt atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga
    13141 gaaaaacgat gtctcattta aaagacccga ccacgcagta ttacactggt gaatatccca
    13201 aacagaaaca accgacgcca ggcatccagg cgaagatgac accgataccg gattgcggcg
    13261 agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg
    13321 attccggaat aggtcgcgct gccgccatcg cttacgcgcg tgaaggggct gacgtggcga
    13381 tcagttatct tcccgtggaa gaagaagacg ctcaggatgt gaaaaagatc attgaagaat
    13441 gcggacgcaa agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc
    13501 tggttcacga agcgcacaag gcgttaggcg ggctggatat tatggcgctg gtcgccggga
    13561 aacaggttgc cattccggat attgcagacc tcaccagcga acagtttcaa aagacctttg
    13621 ccattaacgt tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag
    13681 gtgcaagtgt catcaccact tcgtcaatcc aggcatacca gccaagtccg catttactgg
    13741 actatgcggc tacgaaggcg gcgattctga actacagccg tggcctggca aaacaggtcg
    13801 cggagaaagg tattcgggtg aatattgtcg cgccaggccc tatctggacg gcactgcaaa
    13861 tttccggcgg acaaacgcag gataagatcc cgcagtttgg tcagaaaacg ccgatgaagc
    13921 gtgcggggca accggcggaa ctggcccctg tatatgttta tctggcaagt caggagtcga
    13981 gctacgtcac cgcagaagtg cacggcgtgt gcggcggcga gcatttaggt taaaaaaatg
    14041 cccggttgtg aaaagcaacc gggcatcatt gtgaattcac tcttacctga tgtggcgaaa
    14101 acgccttatc cgcctacaaa cgcatgaaga ttcaacatgt tgcaatagat tttgtaggcc
    14161 tgataagcgt agcgcatcag gcattgagca cctaatgccg gatgcgtcgt gaacgcctta
    14221 tccggcctac aaacgcacga agattcaaca tgttgcaata gattttgtag gcctgatgag
    14281 cgtagcgcat caggcaattc tactttactt cgctttggcg gtttcttcgc cgaccagacc
    14341 tatcttcagg taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc
    14401 gacggttttg tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt
    14461 caacgccgta atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc
    14521 tgccttcacc gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc
    14581 aggcaagttc accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag
    14641 cagaaccaac atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc
    14701 gtcgaggttt tcgttaagat gcattgccat cggacattat cctgcgcgta atttttgtgc
    14761 gacacgaacc ggatgcgcag cggcgctggc ttccagatct aggtcacggc tttgcagcaa
    14821 caatacctgc gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa
    14881 tacgttatag ataacgaccg caggaatcgc tgccacgagg ccgattgccg ttgctaacag
    14941 agcttctgcg atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc
    15001 aataaagctg ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gagaaatcgc
    15061 gccgatggtt gccagatagc cgttaccgcg gcccatttga cgacccactg cggcgacccg
    15121 acgctccaga cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga
    15181 cagttccagc tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa
    15241 atcagcggcg atatcgttgg cctggtttaa ggaacgtgct tcagccagca gttgctgctc
    15301 gcgcttaagg cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca
    15361 ggtgactaca gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgta
    15421 ctgatacata ccccagacgg aaaggtccgt ctgcattaaa ttattaccca ctgtgtatct
    15481 ccaggacgca agtcacaaaa tctgcgcata ataatatcaa aacgacgtcg aattgatagt
    15541 cgttctcatt actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca
    15601 gtaaaaaagt caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg
    15661 atgcgcccgc gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg
    15721 acatggcgga caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca
    15781 ctcttggcgc ggtaaatagc gtgattcagc gcgcttcttc gctggtattt gagagtatgg
    15841 aagccaaaaa gcacgcgacg cgcaatcgcg ccaatggcga gttgttctat ggacggcgcg
    15901 gaacgttaac ccatttttcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct
    15961 gcgcgctatt tccctgcggg gcggcagcgg ttgctaattc gattcttgct tttgtcgaac
    16021 agggcgatca tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca
    16081 aaatcctcag caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata
    16141 tcgttaagca tctgcagcca aacactaaaa tcgtgtttct ggaatcgccg ggttccatca
    16201 ctatggaagt ccacgacgtt ccggcgattg ttgccgccgt gcgcagtgtg gtgccggatg
    16261 ccatcattat gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg
    16321 gcatcgatgt ttctattcag gccgccacca aatatctggt tgggcattca gatgcgatga
    16381 ttggcactgc cgtgtgcaat gcccgttgct gggagcagct acgggaaaat gcctatctga
    16441 tgggccagat ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag
    16501 gtgtgcgttt gcgtcaacat catgaaagca gtctgaaagt ggccgaatgg ctggcagaac
    16561 atccgcaagt tgcgcgagtt aaccaccctg ccctgcctgg cagtaaagga cacgaattct
    16621 ggaaacgaga ctttacaggc agcagcgggc tattttcctt tgtgcttaag aaaaaactca
    16681 atgatgaaga gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt
    16741 ggggcgggta tgaatcgttg atcctggcaa atcaaccaga acatatcgcc gccattcgcc
    16801 cacaaggcga gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg
    16861 tcgacgatct gattgccgat ctggacgccg gttttgcacg gattgtataa cattgccact
    16921 tttggacaat tttgcagaca ttttattgtg aaaagtctta aattgttgcg tccgggatca
    16981 aggcgtcccg gacgattcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca
    17041 ggaaagtcca tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc
    17101 gcgctggcgg atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt
    17161 ttagaaaacg gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca
    17221 ggcgcattga ttgcccaagg ggttatggat tttctgccta cgattgcgat tctgaccgcc
    17281 gcagcaagtc tgggctgctg gctaagttat attcagggga gctggttagg gaataccaaa
    17341 acggtgaaag gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg
    17401 tttgaccgcc acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg
    17461 ctgctgccaa ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac
    17521 tggttaagtg gattgctgtg ggtcagcgtg gtaaccagtt ttggctatgc cttaagtatg
    17581 attccgttcg ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt
    17641 gccttgttaa ccgctggctt gttaggcacg ctgtttgtgg tgattaaaaa aaaatactgt
    17701 aacgcctgac gattttcccc gttcccggct gctgtaccgg gaacgtattt aattcccctg
    17761 catcgcccgc attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc
    17821 acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc gctgctgcgc tggccttcat
    17881 gccgtcatgg atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa
    17941 cggcgaggtg ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc
    18001 ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat ttattctcaa ttcgtttcag
    18061 cacgcggcta atcagactga agtgagtctg gcgactgacc agcgccagta acgcgccgcc
    18121 gcaaggtccg gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg
    18181 cgcatccagt ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc
    18241 tgataacgtg gcggagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc
    18301 gccaatgtcc atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc
    18361 cagcggcacc tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag
    18421 caggtattca ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgccc
    18481 ggaaaagaga attatgatgc caggctcgta catcaccggt gtacgtgcga aaggcgtctc
    18541 gccatacaac aaacgcacat cgggcaacag ttctgacaaa ctattttctt tatttttcag
    18601 tttattaact ttatccgcca gcaagcgaca aatctcttca cgtttcatat cgcgtaattt
    18661 cttaggaata atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg
    18721 gagaaatagg caagacattg gcagaaatga gcattgagag ccagggcgct ggcgatcaca
    18781 atgaaaaaca tcaggcagat cgttctctgc cctcatattg gcccagcaaa gggagcaagt
    18841 aatgaacaac tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc
    18901 tggtttacgc gaacaaattc ctcacgatgc tcgcgtattg attacctacg gtggcggcag
    18961 cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct
    19021 ggaatttggc ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact
    19081 ggttcgcgaa cagaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg
    19141 caccaaattt atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct
    19201 gcaaacgggc ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc
    19261 agcaaccggt tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa
    19321 gcaggcgttc cattctgcac atgttcagcc ggtatttgcc gtgctcgatc cggtttatac
    19381 ctacaccctg ccgccgcgtc aggtggctaa cggcgtagtg gacgcctttg tacacaccgt
    19441 ggaacagtat gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat
    19501 tttgctgacg ctaatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt
    19561 gcgcgccaac gtcatgtggg cggcgactca ggcgctgaac ggtttgattg gcactggcgt
    19621 accgcaggac tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga
    19681 tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgagaccaa
    19741 gcgcgctaag ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgacga
    19801 tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc
    19861 gacccacctc tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact
    19921 ggaagagcac ggcatgaccc aactgggcga aaatcatgac attacgttgg atgtcagccg
    19981 ccgtatatac gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt
    20041 tcgtccagac ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag
    20101 gaggaacgta tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag
    20161 ctgggactgg gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg
    20221 ttagaagtgg gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc
    20281 ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg
    20341 tggaacgacg accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag
    20401 cttgattata tcgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc
    20461 gaagcatgga aaggcatgat cgaattgcaa aaagagggat taatcaaaag catcggcgtg
    20521 tgcaacttcc agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtg
    20581 ataaaccaga tcgaacttca tccgctgatg caacaacgcc agctacacgc ttggaacgcg
    20641 acacacaaaa tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc
    20701 gatcagaaag tcattcgcga tctggcagat aaatacggca aaaccccggc gcagattgtt
    20761 atccgctggc atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgt
    20821 attgccgaaa actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcaaaatt
    20881 gcaaaactcg atcagggcaa gcgtctcggt cccgatcctg accagttcgg cggctaacgt
    20941 gcaaattctc ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc
    21001 cctcgcgtcc cgcccgttgt tactcttcct tgttcaggaa tgccaaatat aaggacatca
    21061 tcatgcagag ccggaagctc ttaaaagaac aactcatcta tatccgggat aaacgcaacg
    21121 gagaggtgaa aaacagatga aaataatact tctgttttta gcagccctgg caagttttac
    21181 cgtacacgcg cagcctcctt cacagaccgt agaacaaaca gtccggcaga tttatcagaa
    21241 ctataaatca gatgccagta cgccttattt cggtgaaacc ggagagcggg cgataacttc
    21301 agcgcgtatt caacaggcgc ttaccctgaa cgacaatctt acactaccgg gcaatattgg
    21361 ctggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgttagaaag
    21421 cgtagcgata acccaaactg acgtcgatca tgccgatgcc gttgtgcgct ttcgtatctt
    21481 taaagatgat aaagaaaaga ccacacagac actgaaaatg gtggcggaaa atggtcgttg
    21541 ggtcattgat gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    21601 tgaaaaaacg ctggccgctt tagcttcgtt gcagaaagaa cagccagaat cctttgttgc
    21661 tgaactcttt gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc
    21721 ttaccgccag gcggttaatg ccttctataa aaccaccttc aagacggcca ataatcccga
    21781 tgaagatatg caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    21841 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    21901 cattcatgtt cgttttacgc tgaccaatgg caacaatgaa gagcaagagc tggttttaca
    21961 gcggcgcgaa ggtaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact
    22021 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaataacaa
    22081 ttagccggaa caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct
    22141 gttgttctgg caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcaactg
    22201 gcgagcaatg tacaaacatt aaatgccaaa atcgcccggc ttgagcagga tatgaaagca
    22261 ctacgcccac aaatctatgc tgccaaatcc gaagctaaca gagccaatac gcgtcttgat
    22321 gcacaggact attttgattg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat
    22381 ccccggcagc atgtcagttg ccggggattt tttttaacgt ccaaccgccg ctttagggcg
    22441 tttcttcgca ccagcattca ccggacgaga ttgcgtagac gacgcttttt ttgccgtagc
    22501 aggcgtctga cgctgagtcg ccatcggcgt atgtttagtc aacgccggac gggtattgcg
    22561 gttctggcga cgagcttcac gcatctcttc aatggttggc gcaggcacta agcaatcgcg
    22621 acggctgcca atcagatgct ttttgcccat cgcttccagc gcctggcgga ttaacggcca
    22681 gtttgccgga tcgtggtaac gcaacaacgc tttatgcaaa cgacgctgtt tgtcgccctt
    22741 cggtacgaag acgccttcgc tcttataacc aatcttcgcc agcggttttt tcccggtgta
    22801 atacatggtg gttgagttcg ccagcggcga tgggtagaag ttctgtacct ggtcgagacg
    22861 gaaacgatgc tttttcagcc acagcgccaa attcaccata tcttcatcac gcgtaccggg
    22921 gtgcgcagag atgaaatacg ggatcagata ctgctcttta cctgcctgtt tcgagtaggt
    22981 atcgaacagc tctttaaagc ggtcatagct gcccatgccc ggcttcatca tcttcgacaa
    23041 cggcccttct tcggtatgtt ccggagcaat cttcagataa ccgccgacgt gatgggtcgc
    23101 caactctttg atatagcgcg gatcttccac ggcgatgtca taacgtacgc ctgaggcgat
    23161 aaggattttt ttaatgcctt tcagatcacg cgcacggcga tagaggttga tcgtcggttc
    23221 gtggttagtg tccatgtgcg gacaaatatc cggataaacg cacgacaaac ggcgacaagt
    23281 ttgttcagcg cgtggcgatt tgcagcgcaa catatacatg ttggcagttg gcccaccgag
    23341 atcggaaatc acgcccgtaa aacctggaac ggtgtcgcgg atcgcttcga tctcattaat
    23401 gatcgaatct tccgaacggc tctgaataat gcgcccttcg tgctcggtaa tagaacagaa
    23461 agaacagccg ccaaagcagc cgcgcataat gttgaccgaa aaacggatca tttcgtaagc
    23521 cggaatacgg gcattgccat aggccggatg tggcacgcgc ttgtacggca gcgcaaaaac
    23581 gctgtccatc tcttcggtag aaagcgggat agcaggcggg ttgatccaca catagcggtc
    23641 accgtgtttt tgcatcaatg cgcgggcgca gcctgggttg gtttcgtggt gcagaatgcg
    23701 cgaagcatgg gcgtacagca ctttatcgcc cttcactttc tcgaaagaag gcagcaatac
    23761 gtaggttttt tcccacggtt tctggcgcgg tggctgcacg attacagctt tcgcttcctg
    23821 ctttttcggc gcaacaggtt tgttatccgc acacggcaaa tcttcgccat acggatgcgg
    23881 gattgggtcg attttcccta gggtatcaag acgggtggaa tccacaccgc tccagccagg
    23941 cagcgcctct tttacgataa ttgcggtatt acgcacatcg cggatttcac taatcggctc
    24001 gcccatcgcc agacgatgcg ctacttccac cagcggacgt tcaccgttac caaacatcag
    24061 catgtcggct ttcgaatcta ccagaacgga acggcgcacg gtatcggacc agtaatcata
    24121 atgcgcggta cggcgcaggc tagcctcaat accgccgagg atcaccggca catctttcca
    24181 cgcctcttta caacgctggg tataaaccag cgtggcgcga tccggtcgct tacccgcgac
    24241 attatctggc gtgtaggcat catcatgacg taaacggcga tcggcggtat agcggttgat
    24301 catcgaatcc atgttgccag cagtaacacc aaaaaacaga ttcggtttac ccagacgcat
    24361 aaagtcgtct ttgctgctcc agtccggctg ggcgatgatc ccgacgcgaa agccctgcgc
    24421 ttccagcata cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat acgcgtcgcc
    24481 agtaaccaaa atgatgtcgc agctatccca gccaagttga tccatctctt cacgagacat
    24541 cggcaaaaac ggtgccggtc caaaacaggc ggcccagtac tgcggccagg agaacaggtc
    24601 gcgatccggt tggatcaggg agatagagct cattttgctt ccagaaatga taaaaaaata
    24661 atcaaaggcc ggggattata agccggaacg aaagagaaat cgaaaggtat tccatactcg
    24721 ccctcctcgg gcgagtatga agattacggt accggattga ccaacagttg cccaatcgag
    24781 ccacggtccg ccatttccag cgtctgactg ttgaagtaga acgggaagtg cgcccaggaa
    24841 ggctgaccga aataaacaag cagctccact tgtccgtcaa cccaaacggt atctttccag
    24901 cctctgtctt ccgggaacgg catcgcgcca ttcacgttac ggatctggaa cattacgcct
    24961 tcaatatgga acgcttgcgg ctcgtccgcg cgtaccgtcc agcgttccca cgttccttgc
    25021 tgcgcggtga catcaatacg gttgacgtcc cacagctgtc cattaatacc cgggtcatca
    25081 cccagactga tatcgcgact gcgaattggc gaaccagcca tgatttcagt tggcagcaag
    25141 cgcatcggaa gactgtctgt gaccagcggc agaaggccgg ttgggcgcag cgttagcacc
    25201 aaggtagaaa ccagaatact ggatggctca aagaagccgc gaatacgatc aacaatgctc
    25261 gccgcttcac cacaggtgat cgacacttca tcgccgttgc tcatatccac cagaatctcg
    25321 cggcgctcgc ccggtgccag cgaaagttgc ttcaccgata caggagcagg caggaatccc
    25381 tgatcgccag aaatcacatg taacgggcga ccatcgctca tctgtagttg atagcgacga
    25441 gagttcgacg cgttcagcag tcgcaagcgc acccagccac gcgagacttc aacgtacggg
    25501 ctttgtacac cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc cggttcgttg
    25561 tattctggcg taccaaagtt atccagccgt ttatcctgga taatgaccgg aaaatcatcc
    25621 acaccataat ggttggggat aggcagcgac ttgctgactt catcttccac cagccacatt
    25681 ccggcaaggc cgttatagac ctgctgagcc gtgcggttgg gagtattggc gtgataccac
    25741 agagtagctg cgttctggcg aataggcagt acgggtgccc agtcagcgtt tggcgacatc
    25801 atccgtgccg gaccgcccat cagcgggcct ggtacctgta gcccggccac cgtcattgag
    25861 acattttctg tcaggcggtt gctgtaaata agcttaacat cgtcgccctt ccagacgcgg
    25921 atagtcggcc ccaggtaacg accattgatt ccccagaccg acgcgcgtgt ccctggcgta
    25981 aatgaccagt gcgcacgttg tacagtcata aacagcggtt gcccacggcg agattcaagt
    26041 agcggcggaa cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag gggaacagcg
    26101 cctgcacaaa gtgcaatccc cgatgcctga atgaactgac gccgactgag tgacatattt
    26161 gctccatgta aaactggcta aagtaatacc gtgcaggaaa gtgttccccg caaaaataca
    26221 gattgccgac ttaaactttt ccggcggctt cgcgttctgc gacttcttta tcgagctcgg
    26281 cgattttttg ttccattatc gaacgacaat gggcagccag ctcacgaacc tgatctttgc
    26341 catactgact gacgtcaatt ggcggcagca tttcgacaat caccagaccg ttgtgcagtc
    26401 gattaagatt aatcttattc gaagttgtag agacgcacac gggaataatc gggacgcccg
    26461 ccgcaattgc cgcgtgaaat gctccagtct tgaacggtag caggccgcga ccacggctgc
    26521 gggttccttc cgggaacatc cagatggaaa tacggcgttt tttgaaatga ttcacgactt
    26581 ccgcaatggt gccgtgagct ttagtgcgat tgtttctgtc gatcaataag ttgccggtta
    26641 accagtacaa ctgcccgaag aaggggatcc acagcaagct ctttttacct accgtcaccg
    26701 tcggcggttg cacaatgttc gatgctgtca ccatgtcata gttgttctgg tggttagcga
    26761 tatagatagc attgccgtag ctttcagcat ctgcaggttt gcggcactca actttcaggc
    26821 cgaacagtgg cgcaagacgg ccaaacatat gcccaaaggt ggccacatgt ttcgggttac
    26881 gcgggctgaa aaggcagtaa atggagccga atacacagac taagatgctg taaatcacgg
    26941 taataataag acgaaagata tatagcataa caccctctga aggtctgaga gactggcatt
    27001 gtacgtcacc tgaaatcagg ttagggcatt gttaaaagcc gctctctgga aaagcgtatt
    27061 gttaatcgca acgcatgaat aaacaacggt ttcaagggaa tttttatcgt ttccgggggt
    27121 tgaatacgct gccggatgac gacttaacgt ttcatccggc gttccttgct agcgggagga
    27181 aacagcgccc tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga
    27241 gtcgatctca acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg
    27301 ttcgccagtg actttctgta gctcttccgg acgcagttta attttgcgtt tcccaacatg
    27361 aatggtcagc gtgctttgcg gcggcagaac gtacagttgc gccagaccat cctcgccacg
    27421 cgcggcttct gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg
    27481 cagatcgctt accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc
    27541 ggaagcatct tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa
    27601 agccttaccc gcacggttac gcgctaccag atcgttaaag gtgcagacga aaccgtaacc
    27661 cgcatcggaa gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt
    27721 cgccccaggc ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg
    27781 cagcgtaatc gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg
    27841 gttactctta cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg
    27901 cgcgtcgata tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt
    27961 gacaggttca gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg
    28021 caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag
    28081 caggttattc attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc
    28141 cagctcactc tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt
    28201 cagttcgagg atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg
    28261 tttcggttca tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac
    28321 cagcaaacct tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg
    28381 gcggcgcacg gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt
    28441 caccgccgga cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag
    28501 atcggtggta gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg
    28561 cacaatcacc agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat
    28621 cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac
    28681 ctgatgcggt aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat
    28741 acgcactgaa ccacgtccgt tctcgtagat tttacgaatc tcggcgcgcg aagtgataat
    28801 ttccgcttca gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtagt
    28861 tttcggctgg tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg
    28921 aatatcggtc gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag
    28981 acgggcaggt agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc
    29041 cgtcccctgc cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt
    29101 gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc
    29161 aaccagcgga taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc
    29221 gctatcgccg tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt
    29281 aaatttggcg ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac
    29341 aggtttcaga ccatcaccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta
    29401 gtttaagtag gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat
    29461 taattctgat tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact
    29521 acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc
    29581 cgacggcgat aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg
    29641 gcatagaggg cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc
    29701 gggcgattcg agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc
    29761 gactgtaagt atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc
    29821 tgccataagt gcggcttgct catggatatc cccgagacgg cttacggcgc accggataat
    29881 aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc
    29941 ctgtgacatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt
    30001 ccccatcacg ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg
    30061 gcaagattgg tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc
    30121 agccagtggc gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg
    30181 cgggccacgg cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg
    30241 ggaatcggtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg
    30301 gcaggggcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc
    30361 tctttacgcc acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg
    30421 tgcggcaaca tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg
    30481 tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa
    30541 ttacggattt actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc
    30601 gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt
    30661 gtgatcgata tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga
    30721 gatgcgttca acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc
    30781 gctttctaca gtcgttacgc tgccgttcgg cccctgaaat cctccggcct gactttgcgt
    30841 taccgatggg ccagaaaaac cgccctgctc tgctgccatc accggtgcac tgcacagggc
    30901 cattactgcg attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt
    30961 ccgagcattt aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttaatg
    31021 ctgtacagag cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga
    31081 aaaaatgcga attttactga tagaagatga catgctgatt ggtgacggca tcaaaactgg
    31141 ccttagtaaa atgggtttta gtgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc
    31201 gctttatagc gcaccttatg atgcggtgat cctggattta accttaccag gcatggatgg
    31261 tcgcgatatt ttgcgcgaat ggcgagagaa aggtcagcgt gagccggtac tgaacctgac
    31321 cgcgcgtgat gcgttagcgg aacgtgtaga agggctgcgt ctgggagctg acgattatct
    31381 gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg gaggctctga tgcgccgaac
    31441 caacggccag gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg
    31501 tatcgccacg ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga
    31561 attactgatg cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta
    31621 tacctgggac gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg
    31681 caaactcggt agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa
    31741 atgaaattta cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg
    31801 gcctcggtga cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc
    31861 gatgaattgt tcgacaccca actgatgctg tttgccaaac ggttaagtac gctcgatctc
    31921 aacgaaatca acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat
    31981 gttgatgacg atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaat
    32041 gatggcgata acggagaaga tattccctat agctatcaac gggaaggttt tgctgacggg
    32101 caactggtcg gtgaagacga tccttggcgt tttgtctgga tgacctcacc tgatggcaaa
    32161 tatcgcatcg ttgttggcca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt
    32221 gccgggcaat tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta
    32281 ctactgggtc gtgaactcgc gccgctgaac aaactggcgc tggcactacg tatgcgtgac
    32341 cctgactcgg aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg gccactggtt
    32401 gagtcgctaa atcaactgtt cgcccgcaca catgcgatga tggttcgaga acgacgcttt
    32461 acctccgatg cagctcacga actacgtagc ccgttaacag cgctgaaagt gcaaaccgaa
    32521 gttgcgcagc tctctgacga tgatccgcag gcgcggaaaa aagcactgct ccaattacat
    32581 tccgggatcg atcgcgctac tcgtctggtt gatcaactgc tcacgctatc gcggctggac
    32641 tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct acaatcgtcg
    32701 gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat
    32761 gcccacggca tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg
    32821 ctggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
    32881 cgcccacccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
    32941 gccaagctgc atgacatgaa tgttgagttt gggaatgcgg aacaaggtgg atttgaggcg
    33001 aaggtaagct ggtaacttct ctgtctatga gagccgttaa aacgactctc atagatttta
    33061 ctaatagcaa aatataaacc gtccccaaaa aagccaccaa ccacaaacca gacaaataac
    33121 gagcgagaat acatatagcc aaaactcgaa catttcccgc ataaagagtt ttcttaagat
    33181 aagaataata agtggcgtaa gaagaaaaaa tgctgcaatc attgcttgca atatacgatc
    33241 ttcgcctgaa acggaaggta tactgcaaat attgcctccc gactcaagca tccagtcata
    33301 cttattgaaa gcaaaaaaag aatctattaa ccaataagta agcattatat atggcaatgc
    33361 ttcaaatatt gttttaattt tctgctttaa catatatcag tacgctcata aaaatccatt
    33421 tataattatc aacatgacac gttgatattt tcatctttta cttattcaaa gccatatttt
    33481 caatccccaa aaaaaccgaa ccaaacgtgt aaattattcc ggtgcgagta agaattatga
    33541 ggaatggcta tcagtattgt cattttcaga aaatatttat cctgcatcgg tgagtcagag
    33601 ctagatcaga cttttgctaa attcgcaaaa gactttgcac attttgctaa tttcacagta
    33661 ccgctctgtg acgtactata gtcgacaaac gtctcacctt gagattaaaa aatgagcaac
    33721 atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact gaacgacacc
    33781 ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgtccg catcgttcgc
    33841 gccgacagcg actacaatgt caaagcggaa gtacaaaact ttctctgggc tgatgtggtg
    33901 atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa atatattgat
    33961 gatgtattca ccgaaggtca cgggactctg tatgccagcg atggtcgtac ccgcaaagat
    34021 ccgtcgaaaa aatacggttc cggcagcctg gtacaggaca aaaaatatat gctttctcta
    34081 acctggaacg caccaatgga agccttcacc gaaaaagatc agttcttcca cggcgttggc
    34141 gttgacggtg tgtatctgcc gttccataaa gcaaaccaat tcctcggtat ggaaccgctg
    34201 ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta tactgaagaa
    34261 tatcgcaagc atcttgtgga aatttttggt taactagagc tcaggcttta gaaggagtta
    34321 accatgctta ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca ccgtcaggcg
    34381 gtattggatc agtttgctaa aatcgttcca accgtactga aagaagaagg ttgccacggc
    34441 tatgcgccaa tggtggattg cgctgctggc gtgagtttcc agtctatggc accggattct
    34501 atcgtgatga ttgagcagtg ggaaagcatc gcgcaccttg aagcgcatct gcaaaccccg
    34561 cacatgaagg cgtacagcga agccgtaaaa ggcgacgtgc tggagatgaa tatccgtatt
    34621 ctgcagccag ggatttaatc ctgccttgtt tgcccggcca tcctgaccgg gcaatgttct
    34681 ttcctttaaa cctcaatctc cgccatgtcg cctttctctt gcaaccagtt gcggcgatct
    34741 tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg atcgtcttca
    34801 tcatcgatag tcaactgcac cagacggcga gtgttcggat caagcgtggt ttcacgcaat
    34861 tgcatcgggt tcatttcccc cagaccttta aaacgctgga cgttcggctt gcctttcttg
    34921 cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta ataaacctct
    34981 ttcccgagat caatacggta gagcggtggc agtgcgacgt aaacgtgacc gtgtttcacc
    35041 aacgcgcgga agtgttttac gaacaaagcg cagagcagcg tggcaatgtg cagaccatca
    35101 gagtccgcat ccgcgaggat acagattttg ccataacgaa gctggctcag atcgtcgctg
    35161 tcaggatcga taccgatcgc taccgaaata tcatgcactt cctgcgaggc cagcacttcg
    35221 tcggaagaaa cttcccaggt gttaaggatc ttacctttca gcggcatgat cgcctgatat
    35281 tcacgatcgc gcgcctgctt ggcagatccg cctgcggagt caccttccac gaggaacagt
    35341 tcggtacggt taaggccctg cgcggtacaa tcagccagtt tgccaggcag cgccgggccg
    35401 ctggtcagct ttttacgcac cactttttta gccgcacgca tacgacgctg ggcgctggaa
    35461 atcgccatct ctgccagcaa ctccgccgcc tgaacgttct ggttcagcca caggatgaag
    35521 gcatctttca ccacgcccga aacgaatgcc gcacactgac gcgaagagag acgctctttg
    35581 gtctgcccgg caaactgcgg atcctgcatt ttaaccgaca gaacataggc gcagcgatcc
    35641 cagatatctt ccgccgacag cttcacgccg cgcggcagaa tgttgcggta ttcacagaac
    35701 tcacgcatcg cgtccaacag gccctgacgc agaccattaa catgggtacc gccctgcatc
    35761 gttgggataa ggttgacgta gctttcggtc agcagttcac cgccttccgg cagccacagt
    35821 agcgcccagt ccacagcttc agtatcgcca gcgaaattac cgataaacgg tttttctggc
    35881 agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc ctgatagcac
    35941 cagcgctgtt cggtgttgtt gatctcatct ttaaaggtaa tctcaacgcc ggggcacagt
    36001 actgctttgg ccttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg gctgtcaaag
    36061 aaggtttcat ccggccagaa gtgcacgctg gtaccggtat tgcgtttacc gcaagtgccg
    36121 acaacctgta aatcctgcac cttttcgcca ttttcaaagg cgatgttata aacctgacca
    36181 tcgcggcgca cgttaacttc tacgcgcttc gacaaggcgt taactaccga aatccccacg
    36241 ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc tgcatgcaga
    36301 cggcaaagaa tcagttcaac cgccggtact ccttcttccg ggtgaatatc caccggcatc
    36361 ccgcgcccat cgtcaatcac ttctaacgac tggtcagcat gtaagattac atccacgcgt
    36421 tttgcgtgac ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg ccccaaatgg
    36481 ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc aagcccggtg
    36541 agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag caattcgagt
    36601 tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat aatcttcgaa
    36661 gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag catagtacgc
    36721 caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc agatcaaatc
    36781 cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg taaatatggc gtgactctag
    36841 cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg tcagcaattc
    36901 aaacgggcgt accgccgggt ttaccaccac tgcgggcagc ataaaacatt gcgacaacca
    36961 ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc cgccatgttc
    37021 caggacaatg gattccagca gttctgccgc gtcggaagga tacggcggca actgcggaat
    37081 gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc tcgcttttgc
    37141 agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca gtagccttct
    37201 gaagcggtat caggttggaa acgtgtgtcc gccaggcgat gcacctcggt ggtcagcgtg
    37261 ccatcagcat gtaactcgag agtacgccag ccgggcgcga tagtatccag cgtaaagttg
    37321 gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaggcg gcgaccgttc
    37381 cagtcgagat ccagctcctg atgaatatga ccgcacagca agtatttgac gtgcggaaac
    37441 ttcgccagca cggtatccag ttcgcccgcg ttacgcagac tgtgttgatc gagccaacta
    37501 caacccgcag gtagcggatg atgatgcagc agcagcaacg tatggcgttc tggcgcatcg
    37561 gccagtttac gttccagcca ctcaagctga aactcgctca gctcaccgtg cggcacgcca
    37621 aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac ccgcttcgcc
    37681 ggggagatac ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc gtggttgccc
    37741 ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg ctgataggcc
    37801 gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa ttcgtgctgg
    37861 tgtggacgaa tcgcctccag caccgcctgg tagctttccc aggtgtttac ccctaacagg
    37921 gcttcgtgct tttgtgcaaa caggtgagta tcggtaattt gtaaaatcct tcctctggcc
    37981 tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt ttcacgacgc
    38041 tagtaaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca gtccgctaaa
    38101 aactggttaa tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg ataatcatac
    38161 cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat cgcgtcatga
    38221 tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc agtctgttct
    38281 attgtcacca gggtagtgta tcgggtcgat tccacaatcg tcagccgata ttgtgcgttt
    38341 gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa acggcgcaat
    38401 tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt gtaacgcttc
    38461 atttatgccc actcattttt taacgcttga tgatgcagct gcagccattg caaagcgatg
    38521 accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg ttcccggctt
    38581 accacatgaa cgcgaatatc ttcgttttca tcagccagac catgaatacc gcttgcggtc
    38641 gtggcgtcca cttcgcccac cataattgac gaacgctcac tggtgccccc cgggcttgcc
    38701 aggaaactta acaccggttt ggtccgtttg actatcagtc ccgcctcttc aatcgcttcg
    38761 cgacgggcaa catcttccac actttcaccc tcttcaatca tcccggcaac catctccagt
    38821 agccaggggg tttcgctggt gtcgtacgcg gcaatccgaa tctgctcaat cagcacaact
    38881 tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc aaaaatttcc
    38941 cgccgtacct catgactcat ttgcccgttg aatagacgat gacgaaatct ataaagatct
    39001 aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc gtttttgcca
    39061 aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctagcact aatagtgaat
    39121 taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatatgtt aaattgaggc
    39181 acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggcttc tgctagaatc
    39241 cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga atgcaaatga
    39301 agaaattgct ccccattctt atcggcctga gcctttctgg gttcagttcg ttgagccagg
    39361 ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa ttgcgtaagt
    39421 ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt ccattactgc
    39481 cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac gcgaacggca
    39541 tcaactcgaa cgcgaccagt gcgtccctgc agttaactca atccattttt gatatgtcga
    39601 aatggcgtgc gttaacgctg caggaaaaag cagcagggat tcaggacgtc acatatcaga
    39661 ccgatcagca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg ttgaatgcta
    39721 ttgacgttct ttcctataca caggcacaaa aagaagcgat ctaccgtcaa ttagatcaaa
    39781 ccacccaacg ttttaacgtg ggcctggtag cgatcaccga cgtgcagaac gcccgcgcgc
    39841 agtacgatac cgtgctggcg aacgaagtga ccgcacgtaa taaccttgat aacgcggtag
    39901 agcagctgcg ccagatcacc ggtaactact atccggaact ggcggcgctg aatgtcgaaa
    39961 actttaaaac cgacaaacca cagccggtta acgcgctgct gaaagaagcc gaaaaacgca
    40021 acctgtcgct gttacaggca cgcttgagcc aggacctgga gcgcgagcaa attcgccagg
    40081 cgcaggatgg tcacttaccg actctggatt taacggcttc taccgggatt tctgacacct
    40141 cttatagcgg ttcgaaaacc cgtggtgccg ctggtaccca gtatgacgat agcaatatgg
    40201 gccagaacaa agttggcctg agcttctcgc tgccgattta tcagggcgga atggttaact
    40261 cgcaggtgaa acaggcacag tacaactttg ttggtgccag tgagcaactg gaaagcgcac
    40321 atcgtagcgt cgtgcaaacc gtacgttcct ccttcaacaa cattaatgct tctatcagta
    40381 gtattaacgc ctacaaacaa gccgtagttt ccgcgcaaag ctcattagac gcgatggaag
    40441 cgggctactc ggtcggtacg cgtaccattg ttgatgtgtt ggatgcgacc accacgctgt
    40501 acaacgccaa gcaagagctg gcgaatgcgc gttataacta cctgattaat cagctgaata
    40561 ttaagtcagc cctgggtacg ttgaacgagc aggatttgct ggcactgaac aatgcgctga
    40621 gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa cagaatgcta
    40681 ttgctgatgg ttatgcgcct gatagcccgg cacccgtcgt tcagcaaaca tccgcacgca
    40741 ctaccaccag taacggtcat aaccctttcc gtaactgatg acgacgacgg ggcttcggcc
    40801 ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt cccccttctc
    40861 gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg tcccggaaga
    40921 caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac tggagcgcac
    40981 gccatctgac accagtcgct ctcgcggttg ccactgtttt tatgctggct ggctgtgaaa
    41041 agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct gcaaacccag
    41101 gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc gaacgtactg
    41161 cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt cagtgtcagc
    41221 aagcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggctcag caatccagcg
    41281 ggagtttctg gatgccgctg atggccggtt acatgatggg gcgtctgatg ggcggcggcg
    41341 cgggatttgc acagcagccg ctgttctcct cgaaaaaccc ggccagtccg gcttacggta
    41401 aatataccga cgcgacgggt aaaaactatg gcgcagccca gccaggccgc accatgaccg
    41461 taccgaagac ggcaatggca ccaaaaccgg cgaccaccac taccgttacc cgtggcggtt
    41521 ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaaccggc acctcttctc
    41581 gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc cggactggcg
    41641 tgagaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc cgtactggtg
    41701 tgaagatgct tactacaagt tgaccctcgc ccaggttgaa aagctggaag aagtcaccgc
    41761 cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg atgagctgat
    41821 gaccaaattc cgcataccaa aacacacctg gagttttgtg cgtcaatcat ggctgactca
    41881 ccagccatcg ctttattcgc gtcttgatct ggcgtgggat ggcactggcg aacctaaact
    41941 tctggaaaat aacgccgata cgccaacgtc actatacgag gcggcgttct ttcagtggat
    42001 ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc agtttaacag
    42061 tctgcaagaa aaactgatcg atcgcttcgt tgagctgcgt gaacagtatg gcttccagtt
    42121 gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggaaccattc agtatttgca
    42181 ggactgcgca accgaagctg aaatcgccac cgagttcctc tacatcgacg agataggttt
    42241 aggcgaaaaa ggccagttca cggatttaca ggatcaggtg atttctaacc tgttcaagct
    42301 ttatccgtgg gaatttatgc tgcgcgaaat gttctccacc aagctggaag atgctggcgt
    42361 acgctggctg gaaccggcgt ggaagagcat tatctccaac aaggcacttc taccgctact
    42421 gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg aagatgatca
    42481 tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg caaacgtgtc
    42541 gatcattgag aacggcaaaa ccattgaagc agcggaaggt ccgtatggcg aagaagggat
    42601 gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc tgattggtag
    42661 ctggctggtg aacgatcaac ctgccggaat tggcattcgt gaagaccgtg cgttgatcac
    42721 ccaggatatg tctcggtttt atccacatat ttttgttgaa taagccacga taccggatgg
    42781 cactcgccat ccggtaattg ttagcctatc tgcaccgaca gcatacttaa actgcccatt
    42841 tctataccat cgaccggaat ggtaattggc tcctgcccat cccacacacc taacacatac
    42901 aacaacggca gatagtgctc tggcgttggg ttcgataacg cgccaccttc atggtcgaga
    42961 taattcacca gaggatgctg ctctaccggc ccttgccacg tcagattcgc tttcacatac
    43021 tcattaaacg acgtcgccca cggatacggt gaactatcac cgtgccactt cactgtgcgc
    43081 aggttatgca ccacgttacc gctggcgacc aacattattc cttcatcacg cagcgccgcg
    43141 agtttgcgcc ccatttcgaa atgccaggcg gcaggtttgc tactgtcgat actcaactgc
    43201 accataggga tatcggcgtc aggatacatc ttaatcagca cgccccacga gccgtggtca
    43261 aagccccagg cttctttatc cagcgtcacc gggatcggcg ctaacagctc aaccagacgc
    43321 tgtgccagcg caggcgaacc cggagcagga taatgcgtat cgtacagcgc ctgcgggaag
    43381 ccaccaaagt catgaatcgt gggcggcgtc tccatcgcgg tcactcctgt tccacgggta
    43441 aaccagtgag ccgaaaccac cacaatcgct tgcgggcgtg gcaatgtcat ccccaacttc
    43501 tgccagctgc gggtatacaa attatcttcc agcacgttca tcggactacc gtgacctaaa
    43561 aacaatgctg gcatacgtgt tgaagacatg atgatatcct taactaaagg tgtcattttg
    43621 atatcctcac aatacgcttg ttcggcggag taagaacccg gataacaatg atgatgatca
    43681 tcagttattt tgacgatctg cctgaaggtg aagatttata aggagttgtc gatgtcagta
    43741 cctctcattc tgaccatact ggcgggggca gccacgttta ttggcgcgtt tctcggcgtt
    43801 ctcggacaaa aaccctcgaa ccgcttactg gcgttttcgc taggttttgc ggcggggatc
    43861 atgttgctca tctcattaat ggaaatgctt cctgccgcac tagcagctga aggaatgtcg
    43921 ccggtgttgg gttatggaat gtttatcttc ggtctgcttg gctactttgg cctggaccgc
    43981 atgttgccgc atgcgcatcc gcaggattta atgcaaaaat cggtgcagct gttgccaaaa
    44041 tcgatcaagc gcacagccat tctgctcact ctcggcatca gtctgcataa cttcccggaa
    44101 gggattgcca cctttgtcac ggcgagcagc aacctggagc tgggatttgg catcgcactg
    44161 gccgtcgcgt tgcacaatat ccctgaaggt ctggcagtgg caggcccggt ttatgcggca
    44221 acggggtcta aacgcaccgc gattctgtgg gcggggattt ctggactagc agaaattctt
    44281 ggtggtgtgc tggcgtggtt aatcctcggt agcatgattt ccccggtggt catggcggca
    44341 atcatggcgg cggttgcagg aattatggtg gcgctctcgg ttgatgaatt aatgccgctc
    44401 gccaaagaga ttgaccctaa taataacccc agctatggcg tactatgtgg aatgtcagtg
    44461 atgggattca gtttagtgct gctacaaacg gcgggaattg gttaaaaaca acatcagaag
    44521 ttgtccagca catggacaac ttctgccagc ttcacgaaag ccttattcta ccttttcggc
    44581 tgtctcttct ctcgtactgt ttaagtattt gttccgcgct tcaagcagtg cgtctctgtc
    44641 ctggcgaaac gcccgacaat aacgtttcaa atgcaggaaa taacccacca caaccagagc
    44701 aatcaataca atccagtacc aggcgataaa catcctttta cccttaatat ttaacaatac
    44761 taaacattta gcgcataaat ttcacatatc ctttttcgga tatattcata tggtcgcata
    44821 ataacggaac aattatatga ttttgatcat aatgaaaaag aatgcctgat attaatgaaa
    44881 ttttctcata gaaagagaag cggaattaac atcttgcata gcagggcaaa ataaaaaccg
    44941 aagccagcta caacttcggt ttcttgatta aggcagtaat taagcagcgg ttttcagctg
    45001 gctttacgct catgtgcctg acggtatgcc accaggtctt caatagtcac gagcgccata
    45061 ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc atcgtcatta
    45121 gtcagctcac acagcacacc agccggttta aagcctgcca gcgtcatcaa atcaatagtc
    45181 gcttcagtat gaccgccacg cgtcagtaca ccacctgcct gcgcgcgaag tgggaaaacg
    45241 tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc gcgaacggtc
    45301 gtaatacgat cagcggcaga aacaccggta gtcacacctt cagccgcttc aatggtcacg
    45361 gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag atcaagttgt
    45421 ttacggcgat cttcagtgat gcacaggcaa acaataccgc taccgtggcg aatggtcagc
    45481 gccatctgct caacggtcat ggtttctgcc gggaagatca tatcgccttc gttttcacgg
    45541 tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcagccag tgcattttca
    45601 acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt aaaaaaacct
    45661 cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat atagccaaaa
    45721 taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg actctaaccg
    45781 tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg
    45841 gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg agaataagcg
    45901 gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata attctggttt
    45961 atgacttacc cttatcgcac tacaatggta ctcaacacct atcagtacag ggaaactaca
    46021 atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa
    46081 ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct acaagctcag
    46141 ctgacgcgcc tcgaccttgt aagccgtgaa gagttcgacg tccaaacgca agtcctgtta
    46201 cggactcgtg agaaactggc gctgcttgaa caaagaatca gcgaactgga aaatcgcagt
    46261 actgagatca aaaagcagcc agatccagag actctccccc caacgctgta aacatttgtc
    46321 tttaatgcac tgtaaattat gaattaatcc tctaatttac agtgttttct attacattat
    46381 cgcttaatcc cattcagagt atcatcacaa aaatgaatga ttgcaattga aaatataatt
    46441 gttatgtatt ctttaagttt tacttaatcg ata
//
LOCUS       DAZPEU010000012        46464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPEU010000012 DAZPEU010000000
VERSION     DAZPEU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.012"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            317..442
                     /gene="timP"
                     /locus_tag="ACHU2L_000580"
     CDS             317..442
                     /gene="timP"
                     /locus_tag="ACHU2L_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR1355572.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(474..1250)
                     /gene="sseB"
                     /locus_tag="ACHU2L_000581"
     CDS             complement(474..1250)
                     /gene="sseB"
                     /locus_tag="ACHU2L_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR1355573.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1392..2675)
                     /gene="pepB"
                     /locus_tag="ACHU2L_000582"
     CDS             complement(1392..2675)
                     /gene="pepB"
                     /locus_tag="ACHU2L_000582"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR1355574.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2734..2934)
                     /gene="iscX"
                     /locus_tag="ACHU2L_000583"
     CDS             complement(2734..2934)
                     /gene="iscX"
                     /locus_tag="ACHU2L_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR1355575.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2946..3281)
                     /gene="fdx"
                     /locus_tag="ACHU2L_000584"
     CDS             complement(2946..3281)
                     /gene="fdx"
                     /locus_tag="ACHU2L_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR1355576.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3283..5133)
                     /gene="hscA"
                     /locus_tag="ACHU2L_000585"
     CDS             complement(3283..5133)
                     /gene="hscA"
                     /locus_tag="ACHU2L_000585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR1355577.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5150..5665)
                     /gene="hscB"
                     /locus_tag="ACHU2L_000586"
     CDS             complement(5150..5665)
                     /gene="hscB"
                     /locus_tag="ACHU2L_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR1355578.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5761..6084)
                     /gene="iscA"
                     /locus_tag="ACHU2L_000587"
     CDS             complement(5761..6084)
                     /gene="iscA"
                     /locus_tag="ACHU2L_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR1355579.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6101..6487)
                     /gene="iscU"
                     /locus_tag="ACHU2L_000588"
     CDS             complement(6101..6487)
                     /gene="iscU"
                     /locus_tag="ACHU2L_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR1355580.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6515..7729)
                     /gene="iscS"
                     /locus_tag="ACHU2L_000589"
     CDS             complement(6515..7729)
                     /gene="iscS"
                     /locus_tag="ACHU2L_000589"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR1355581.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7841..8329)
                     /gene="iscR"
                     /locus_tag="ACHU2L_000590"
     CDS             complement(7841..8329)
                     /gene="iscR"
                     /locus_tag="ACHU2L_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR1355582.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8599..9339)
                     /gene="trmJ"
                     /locus_tag="ACHU2L_000591"
     CDS             complement(8599..9339)
                     /gene="trmJ"
                     /locus_tag="ACHU2L_000591"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR1355583.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9458..10261
                     /gene="suhB"
                     /locus_tag="ACHU2L_000592"
     CDS             9458..10261
                     /gene="suhB"
                     /locus_tag="ACHU2L_000592"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR1355584.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            10406..11260
                     /locus_tag="ACHU2L_000593"
     CDS             10406..11260
                     /locus_tag="ACHU2L_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1355585.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11451..12731
                     /gene="csiE"
                     /locus_tag="ACHU2L_000594"
     CDS             11451..12731
                     /gene="csiE"
                     /locus_tag="ACHU2L_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR1355586.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12723..13862)
                     /locus_tag="ACHU2L_000595"
     CDS             complement(12723..13862)
                     /locus_tag="ACHU2L_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR1355587.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14022..14912)
                     /gene="hcaR"
                     /locus_tag="ACHU2L_000596"
     CDS             complement(14022..14912)
                     /gene="hcaR"
                     /locus_tag="ACHU2L_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR1355588.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15048..16409
                     /gene="hcaE"
                     /locus_tag="ACHU2L_000597"
     CDS             15048..16409
                     /gene="hcaE"
                     /locus_tag="ACHU2L_000597"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR1355589.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16406..16924
                     /gene="hcaF"
                     /locus_tag="ACHU2L_000598"
     CDS             16406..16924
                     /gene="hcaF"
                     /locus_tag="ACHU2L_000598"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR1355590.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16924..17244
                     /locus_tag="ACHU2L_000599"
     CDS             16924..17244
                     /locus_tag="ACHU2L_000599"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR1355591.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17241..18053
                     /gene="hcaB"
                     /locus_tag="ACHU2L_000600"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17241..18053
                     /gene="hcaB"
                     /locus_tag="ACHU2L_000600"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR1355592.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18063..19280
                     /gene="hcaD"
                     /locus_tag="ACHU2L_000601"
     CDS             18063..19280
                     /gene="hcaD"
                     /locus_tag="ACHU2L_000601"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR1355593.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            19377..19799
                     /locus_tag="ACHU2L_000602"
     CDS             19377..19799
                     /locus_tag="ACHU2L_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1355594.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19847..20719)
                     /locus_tag="ACHU2L_000603"
     CDS             complement(19847..20719)
                     /locus_tag="ACHU2L_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1355595.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20731..21825)
                     /locus_tag="ACHU2L_000604"
     CDS             complement(20731..21825)
                     /locus_tag="ACHU2L_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1355596.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21858..22856)
                     /locus_tag="ACHU2L_000605"
     CDS             complement(21858..22856)
                     /locus_tag="ACHU2L_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1355597.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22881..24392)
                     /locus_tag="ACHU2L_000606"
     CDS             complement(22881..24392)
                     /locus_tag="ACHU2L_000606"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1355598.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24415..25397)
                     /locus_tag="ACHU2L_000607"
                     /pseudo
     CDS             complement(24415..25397)
                     /locus_tag="ACHU2L_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(25494..28775)
                     /locus_tag="ACHU2L_000608"
                     /pseudo
     CDS             complement(25494..28775)
                     /locus_tag="ACHU2L_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            28893..30086
                     /locus_tag="ACHU2L_000609"
     CDS             28893..30086
                     /locus_tag="ACHU2L_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR1355599.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30284..31537)
                     /gene="glyA"
                     /locus_tag="ACHU2L_000610"
     CDS             complement(30284..31537)
                     /gene="glyA"
                     /locus_tag="ACHU2L_000610"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR1355600.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31864..33054
                     /gene="hmpA"
                     /locus_tag="ACHU2L_000611"
     CDS             31864..33054
                     /gene="hmpA"
                     /locus_tag="ACHU2L_000611"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR1355601.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33099..33437)
                     /gene="glnB"
                     /locus_tag="ACHU2L_000612"
     CDS             complement(33099..33437)
                     /gene="glnB"
                     /locus_tag="ACHU2L_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR1355602.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33498..34832)
                     /gene="glrR"
                     /locus_tag="ACHU2L_000613"
     CDS             complement(33498..34832)
                     /gene="glrR"
                     /locus_tag="ACHU2L_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR1355603.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34822..35535)
                     /gene="qseG"
                     /locus_tag="ACHU2L_000614"
     CDS             complement(34822..35535)
                     /gene="qseG"
                     /locus_tag="ACHU2L_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR1355604.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35700..37127)
                     /gene="qseE"
                     /locus_tag="ACHU2L_000615"
     CDS             complement(35700..37127)
                     /gene="qseE"
                     /locus_tag="ACHU2L_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR1355605.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37685..41572)
                     /gene="purL"
                     /locus_tag="ACHU2L_000616"
                     /gene_synonym="purI"
     CDS             complement(37685..41572)
                     /gene="purL"
                     /locus_tag="ACHU2L_000616"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR1355606.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            41831..43387
                     /gene="mltF"
                     /locus_tag="ACHU2L_000617"
     CDS             41831..43387
                     /gene="mltF"
                     /locus_tag="ACHU2L_000617"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR1355607.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43384..43887)
                     /gene="tadA"
                     /locus_tag="ACHU2L_000618"
     CDS             complement(43384..43887)
                     /gene="tadA"
                     /locus_tag="ACHU2L_000618"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR1355608.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(43945..44580)
                     /gene="yfhb"
                     /locus_tag="ACHU2L_000619"
     CDS             complement(43945..44580)
                     /gene="yfhb"
                     /locus_tag="ACHU2L_000619"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR1355609.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            44789..45637
                     /locus_tag="ACHU2L_000620"
     CDS             44789..45637
                     /locus_tag="ACHU2L_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR1355610.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(45976..46464)
                     /locus_tag="ACHU2L_000621"
                     /pseudo
     CDS             complement(45976..46464)
                     /locus_tag="ACHU2L_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11041 a        12783 c        11691 g        10949 t
ORIGIN      
        1 aaccgccaaa atcaggcggt tatttgttgc tggtccggtt cgcggccttt ccagcaggtt
       61 gtattaccgt agtaatgcaa gcgcgtctca gcggagacaa tactcgccag taactctctt
      121 tttgtcaagc aaaagagagt tattattgtt ctgttagtgt attatccact gcggcccttt
      181 ccgccgtctc gcaagcgggc gctggcttaa ggaaaggatg ttccgcgacc gttagagcaa
      241 gcgcgtctca gcgcttgcta tcgcggcaat atcgccagtg gtgctgtcgt gatgcggtct
      301 tcgcatggac cgcacaatga agatacggtg cttctgtatc gtacttattg tttctggtgc
      361 gctgttttcc caagtaaata ataaccggag tctctccggc gacaatttac tggtggttaa
      421 caaccttcag agcagcaagt aagcccgaat gccgcccttt agggcggcat attttagatt
      481 atccggtttt gtttaaagtc acgcaaaaaa ccaccccagc gacgttcata gaatggcgca
      541 atatgttcgg taataaagtg gctaattcct ttttcccctt ttttcacctg acaaatatcg
      601 attggttcat cgccaggtaa tgtatcggtc gctacacttc ccgtcgcctg aataatttct
      661 tcgatatcac catcggcttc aatgccaata agtaaattag gctgtgcctc ttcgttctct
      721 ttaattgaac aaataaaagc acgcttcacc ggcttaatgg ttttaaataa ggtggtgagt
      781 gaatcaatca tttgtgctgg cggctctgcg acttccgata atatcagcga ttcaccgcct
      841 tccaggattt cctggctgct cagcggattt ccctcttcac caatcaacaa actgatttca
      901 cgcggcataa attctttacc ggtcggcagt ttggcattaa ggaagagcgt ttcgccaagg
      961 gtcatctcaa acaatgtgcg aacgggcatt acgacaaatg cctgttcgtc ttcaaccgcc
     1021 tgttgaagcg cttctaacga ggtgaaaaaa ggaatgacgc tggtgccgtc ttctttttcc
     1081 cagtgctgta aatcaagcgc actatcttca accacagcct cgccctgcgc cgccgtacca
     1141 ggcacccaga cggtggattc cagtagagta cggaaaaagg ccgggcggtg cgccggttcg
     1201 gttgctgctt tttccagcag gtcttcaagt tcgttttttg tttcggacat aagaaccaca
     1261 attcattcaa cgttcggggc gcaaatgcct gatgcgctac gcttatcagg cctacaaggg
     1321 attcgcaatt tgttgaattt gcagaatttg taggccggat aaggcgttta cgccgcatcc
     1381 ggcatattag tttacgccgt taacagatta gctatcgtgc gcacaccaag tcccgtagcg
     1441 cccgcagacc actgttcaac cggcgcttta cggtaagtcg ccgagcagtc gatatgcagc
     1501 cagccttgct gatagttctc aacaaagtgc gacaggaagc ccgccgccgt gctcgcgcct
     1561 gccggatacg ccgcgcttcc ggtattgttc agttcggcaa agttagacgg cagctggctg
     1621 cggtggaatt ccgccagcgg cagacgccag aatggttcgt tctcttgtga ggcactcgcc
     1681 agtaaacgac cggcaagcgc atcgtcaaaa ctgaacagcg cgtgataatc attacccagc
     1741 gcagttttcg ccgccccggt gagggtcgcc gcatcaatga tcatttccgg tttctgcgca
     1801 ctggcatcaa tcagaccatc ggcaagcacc agacgccctt cggcatcagt gttcatcact
     1861 tcaacttttt taccgttgcg ataggtgatg atatcgccca gcttgaacgc attgccgcta
     1921 atcaggttat ccgcacagca gaggaacagc ttcacgcgct tgttcagtcc gcgcataatg
     1981 gcaaatgcca gcgccccggt aaccgttgcc gcgccgccca tgtccgactt catcgagtcc
     2041 ataaacgcag tctgtttgat gctgtagccg ccggagtcaa aagtgatacc tttacctacc
     2101 aggcacgcgt acaccggtgc ttctttatcg ccagttgggt tgtaatccag cgccagcaat
     2161 accggagaac gttctgaacc gcgcccgaca gtgtgcagcc ccatataacc ttgctcacgc
     2221 agatcttcgc ctttggtgat ccgataagtc acacgatcgc ccgcgacgtt gctgatcaga
     2281 tcaacagcac gctgtgccag ttgcgatggt cccaattctt ctgccggtgc gttgatggtg
     2341 tcacgcaccc agtcgatgat catcaggcgg ttatccagtt cctggcgctg ggcatcgtcc
     2401 agatccggcc actccacttt acgcgtgcct ttcgggcctt tataaccttg ccagaatgcc
     2461 cagcagcgat ccgcatccca gccttcaccg cttaactgaa catgcttgat gcccagaccg
     2521 tcaatcttgc gggccgcacg ctggatcagc cccagatcgt ctgccccgtt caaatgcagg
     2581 gtaatgccgt cattattaat gctgtaagtt gctttttctc cccagcgcgc gtcggcaggt
     2641 tgggtagaga gggtaatctt catcgcttct gtcattttag ttatccttat tcgtttaaca
     2701 gcaaaacggg ctacccccag gcagcccgtt cattcactcg gcttcgtcca gccagactaa
     2761 caaaatcgct tcgaggattt tctcgttgga tgcctgcggg tcgtcgtcga aatcttccag
     2821 atcgcaaatc cactgatgca tatcggtgaa tcgaaccgtt ttcggatcaa gatcgggata
     2881 cgcatcgtac agtgcttcgc caatttcgcg gctatcggtc cacttaagtc ccatactaac
     2941 ctctgttaat gctcacgcgc atggttgata gtgtaacgcg ggatttcgac cactaaatct
     3001 tcgtcggtga cgcgcgcctg gcagcttaaa cggctttccg gctccagtcc ccaggctttg
     3061 tccagcatgt cgtcttcctg ctctgagctt tccggcagtg agtcaaaacc ttcacgaacg
     3121 atgcagtggc aggtggtgca agcacaggat ttttcacagg cgtgttcaat ctcaataccg
     3181 ttacgcagcg ctgcgtcgag aatggtttca ccgctattag cttccagaac agcgccatca
     3241 gggcagaggt cctgatgagg caaaataaca atctttggca tattaaacct cgtccacgga
     3301 atggcctttc agcgcacgac gaaccgactg gtccatgcgg cgagcggcga aatcctgggt
     3361 ttgtttgtct acgtttttaa tcgcttgttc gatggcgtca acatcatcgc cctgcgccac
     3421 ttcactcagg tgagcggcag cattgtcaat gacctgacgc tctgcggcgc ttaacagcgc
     3481 ggcatcagca gccagcgcgc cgtgcagact ttccagcaca cgcgccgctt ctactttttg
     3541 ttctgccagc atacgggctt ttacgtcctg ctcggcatag ctcattgagt ctttgatcat
     3601 cgaagcgatt tcgctatcgg tcagaccgta agacggtttg acctgaatag acgcctcaac
     3661 gccggtggat ttctccatcg ccgtcacgct caaaagaccg tcggcatcga cctggaacgt
     3721 cacgcgaata tgcgcaccgc cagccggtag cgccggaata ccacgcagcg caaaacgcgc
     3781 cagtgagcgg cagtcctgca ccagttcgcg ctcaccctgc attacatgga tagacatcgc
     3841 cgtctgacca tctttaaagg tggtgaaatc ctgagcgcgg gccaccggaa tagtggtatt
     3901 acgcggaatc actttctcca ccaggccgcc catcgtttcg aggcccagcg acagtgggat
     3961 cacatcaagc agcagcattt cgctgtctgg cttgttaccc accagaatat ccgcctgaat
     4021 cgccgcgcca atagcgacga ctttatccgg gtcgatggaa gtcagcggtg gacgaccgaa
     4081 aaattcgcct acccgttcac gcaccagcgg cacgcgagta gaaccgccca ccatcaccac
     4141 ttccagcact tcgtcagctt ctacacccgc gtctttcagc gcgcgacgac aagccagtaa
     4201 ggttcgtttt accagtggcg cgatcagttc attgaattgt tcacggctga tttcgccctg
     4261 ccagcccgca acgttaacgg tcacggagtc cgcatcgctc agcgcgattt tggctgcaat
     4321 ggtggcatcc agcagttcac gctgaacgcg gttatcgcta cgatcaggaa tgtccgcctg
     4381 ctcgcgaatg taatccgcca gcagatggtc gaaatcatcg ccgccgagcg cggaatcacc
     4441 gccggttgcc agcacttcaa acacgccgcg acttaagcgc agaatggaaa tatcaaacgt
     4501 cccgccaccg aggtcataaa cagcgatcac gccttcctga ccggaatcca gcccgtaggc
     4561 gatagccgca gcagtcggtt cgttaagtaa gcgcagaaca tgcaaccccg ccagacgcgc
     4621 cgcgtctttg gtgccctgac gctgggcatc gtcaaagtac gccggaacgg tgataactac
     4681 accatccagc tcgcctgcca gggcttcagt tgcccgcgcc gccagtgctt tgaggatgtc
     4741 cgcagaaacg cgcaccgggt tcagtagccc cgccgccgtt tcaatcatcg gcaggccgtt
     4801 ttcgctggcc tggaattgat aaggcagatg cggatagcgt tgctggatat cagccagcga
     4861 gcgccccatc aggcgtttaa cagaactaat ggtgttggcg gtgtcaagcg ctgcgttagt
     4921 acgcgcgtca taacccaccg aatgcccttg ctgttgatag tgaacaacag atggcagcag
     4981 gtgacggcct tcatggtcgg ctaacgtttc ggcctgaccg ctgcgcactg tcgccaccag
     5041 cgagttggtt gtgcccaggt caataccagc cgccagacga cgctgatgcg gcgcagcact
     5101 caaaccaggt tcactaattt gtaataaggc catgtttagc ttccagaaat taaaaatcga
     5161 gcagtttttc ttcgagttgt tcggcactgc tccgcagttt atcgagaaaa cgcagcttac
     5221 gcaccgtatc cgccgccgcg tcccacgcct cgttgtctaa ctgttcaacc atcaactgat
     5281 ggcgggtatc aaacatcttt ttcacacgtt tgataaagct ttccagccgc gcttcatctt
     5341 tcgcctgttc gatctcgtcc agctcttcgc gcagctccaa ctgttccatc aggaacgcgg
     5401 tgtcgcgcac agtatgctgc tcgctggcga gatcaaaacc gtgtaaagaa agcaaatatt
     5461 ccgcgcgcat taacggatga cgcagcgttt gccaggcctg gttaatggtt gcagattgct
     5521 gtacggcggc gagttgttcc gccaggcttc cgctggcgaa tttatcagga tgatactgac
     5581 gttgtagatc ctgaaaacgc aggctcagtg cctgggtatc gagttgataa cgggcaggca
     5641 agccaaagag ggtgaagtaa tccataacat tctcagggct gcgatccgcc gcgataacgg
     5701 cgaatcaggt aaaacaaacc ccacgcgcag gcgaccacgg tggggttatc ggtatgcgca
     5761 tcaaacgtgg aagctttcgc cgcaaccaca ctcatcttta acgttcgggt tggtgaattt
     5821 aaacccttca ttcaggcctt cttttacgaa gtccagctgc gtaccgtcca gaaattgcag
     5881 gcttttgcca tcgaccacga ctttcacgcc tttgtcttca aacacgatgt cttccggcgt
     5941 cggttcgtca acaaattcca gtacataagc catacctgaa catccggagg ttctcacgcc
     6001 cagacgcagg ccaaaccctt tgccgcggtt agccagaaag gtatttactc gcgctgctgc
     6061 actgtcgctc agtgtaatcg acataaccaa acctcaactc ttattttgct tcacgtttgc
     6121 ttttatagtc cgcaatggcg gctttgatcg cgtcttctgc cagaatagaa cagtgaattt
     6181 tcaccggcgg cagttcaagt tcttcagcaa tatcggtgtt tttgatcgcc tgcgcttcgt
     6241 cgagagactt ccctttcacc cattcggtga ccagggagct ggaagcgata gcggaaccgc
     6301 agccgtaagt tttaaaacgc gcgtcttcaa tgataccttc atcgttgact ttaatctgca
     6361 acttcattac gtcgccacag gccggtgcac ccaccatgcc gctgccgacg ttctcgtcgt
     6421 tgttatcaaa ggaacccacg ttacgcggat tctcgtaatg gtcgataact ttttcgctgt
     6481 aagccattat aaattctcct gattccgata ccgattaatg atgagcccat tcgatgctgt
     6541 tcagatccac gccctgcttg tacatttccc acagcggaga aaggtcacgc agacgaccga
     6601 tggatttacg aactaactcg atggtgtagt cgatctcttc ttcagtagta aaacgaccta
     6661 aagagaaacg gatagagcta tgtgccagct cgtcgttcag ccccagcgcg cgcagcacgt
     6721 aggacggttc gaggcttgct gacgtacagg cggaacctga agaaactgcg aggtctttca
     6781 gcgccataat cagcgactca ccttcaacgt agttgaagct gacgttgaga atgttcggcg
     6841 caccgtgttc caggtcaccg ttcaggtaaa cttcttcgat atctttgatg ccgttccaca
     6901 gacggttacg caggccgcgc agacgttcca tctcggtcgc catctcttct tttgcgatgc
     6961 gataggcttc gcccattccg acgatctggt gaacaggcag agtgccggaa cgcataccgc
     7021 gctcgtgacc gccgccgtgc atttgcgctt cgatgcgtac acgcggttta cgacgcacat
     7081 acagcgcacc gatacctttc gggccataga ttttgtgacc ggagaaagac atcaggtcaa
     7141 ctttcaactg gctcaggtcg attggcagtt tgcccacgct ctgggttgca tcaacgtgat
     7201 agataatgcc acgagcacgg cacatttcgc cgatagccgc gatatcctgc accacgccga
     7261 tttcgttatt tacgtgcatg atggaaacaa ggatggtgtc gtcacgcatc gccgcttcga
     7321 gttctttcag gtcgataata ccgttacgct gcggtgccag gtaggtgact tcgaaacctt
     7381 cgcgctccag ctgacggcaa gtatccagta ccgctttgtg ttcggttttg ctggtgatga
     7441 tgtgcttgcc ttttttctga taaaagttgg ctgcaccttt gatcgccagg ttgtcagatt
     7501 cggttgcacc agaggtaaag acgatttcac gcggatcagc gccgaccaga tcggcaatct
     7561 gattacgggc gatatctacc gcttcttcag cctgccagcc gaaacggtga gaacgagagg
     7621 ccgggttacc aaaggttccg tccatcgtca tgaactgcat cattttctcg gcaacacgcg
     7681 ggtccaccgg cgtggttgcg gagtagtcga gataaatcgg taatttcatt gctctataaa
     7741 ctccgtacat cactcaatgc aaggaatcag gctaccggct ggatgtacga ccgtgtttac
     7801 ggagtattta gcactccggc ctgattctga attcttttta ttaagcgcgt aacttaacgt
     7861 cgatcgcgtc ttgtgtgcgg gtgcgtggcg cgtcgtgagt atgctgacga ccagacacat
     7921 ccagcacttc ctggttatta accagttcgc ctaaagtaat gttgttgaga aaaccggtga
     7981 gacggtcgct caaatcacgc cacagcgcgt gggtcaggca tttatcgccg ccctggcagc
     8041 cgcctttacc ctgacaacgg gtggcatcta cagattcgtc aacggcgcta atcacttcgc
     8101 caacggcgat gctgctggca tctttgccta acagataacc accgcctggt ccacgtacgc
     8161 tggaaaccag accattttta cgcagacggg aaaacagttg ttccagataa gaaagggaaa
     8221 ttccctgacg ttcggaaata tcagccaacg gtaccgggcc cgcttcagag ttgagcgcaa
     8281 cgtcaagcat tgcggtcacg gcatagcgcc ctttagatgt cagtctcatg tcttacttca
     8341 cctcaaactc gcccctgccc ggggtttttt attgtaaaag tgggggtatt gcatagcagg
     8401 gtcaagtctg acattcccga gtaaattggt caactattta cttgactgat ttagtcgggt
     8461 atttaacctt cagtgccatt tttttatcgt ggcgtttgta cctgttgtcg gatgcggtgt
     8521 gaacacctta tccgacctac gattctgtac attgtaggcc tgataagacg cgttaagcgt
     8581 cgcatcaggc aaccactgtt attcggcctt gttaccttta ttctgctgct caatagaagc
     8641 cagaatcccg cgcaggatat tcaattcctg gctttccggg cgcgcacggg taaacagacg
     8701 gcgcaattta ttcatcacct gccccggatg gttttcacgg ataaaaccgg ttgccagcag
     8761 cgtttgttcc agatgaccat aaaaacgctc cagatcatcg accagcggat acggcgtctc
     8821 ttcatgctcg acctgctcgc cattttcctg agttgccagc caggccatac gcacttcata
     8881 ggcaataacc tgaaccgcca ttgccaggtt cagcgaactg tattccgggt ttgccgcaat
     8941 cgcgacatga taatggcatt tctgcaactc ttcattggtc aagccgacgc gctcacgacc
     9001 aaacaccagc gccaccgggg tatttgccgc ttcagcgacg cttttcagac cgcattcgcg
     9061 cgggtcgagc atcggccacg gcagcgtgcg ggagcgtgcg ctggtgccca ccaccaggct
     9121 acaaccggct aacgcttcgt cgagcgtatc gacgatgtga gcattaccaa tcacatcgct
     9181 ggcccctgca gccagggcaa tcgcctggga gtcgggtttc accagtggat taaccagcca
     9241 cagattggtt aatcccattg ttttcatggc acgggcaaca gaacccatat tgccggtgtg
     9301 tgacgtctcc accagcacaa ttcgaatatt ttgcagcatt gtctttcttc atctaaagat
     9361 tattcacgca tcttatcata aaacgaagac agatgccgat ctcgctgcta tactctgcgc
     9421 cgttttcccg ttctttaaca tccagtgaga gagaccgatg catccgatgc tgaacatcgc
     9481 cgtgcgcgca gcgcgcaagg cgggtaattt aattgccaaa aactatgaaa ccccggacgc
     9541 tgtagaagcg agccagaaag gcagtaacga tttcgtgacc aacgtagata aagctgccga
     9601 agcggtgatt atcgacacga ttcgtaaatc ttacccacag cacaccatca tcaccgaaga
     9661 aagcggtgaa cttgaaggta ctgatcagga tgttcaatgg gttatcgatc cactggatgg
     9721 tactaccaac tttatcaaac gtctgccgca cttcgcggta tctatcgccg ttcgtatcaa
     9781 aggccgcacc gaagttgctg tggtatacga tcctatgcgt aacgaactgt tcaccgccac
     9841 tcgcggtcaa ggcgcacagc tgaacggcta ccgtctgcgc ggtagcaccg ctcgcgatct
     9901 cgacggtacc attctggcga ccggcttccc gttcaaagca aaacagtacg ccactaccta
     9961 catcaacatc gtcggcaagc tgttcaacga atgtgcagac ttccgtcgta ccggttctgc
    10021 ggcgctggat ctggcttacg tcgctgcggg tcgtgttgac ggtttctttg aaatcggtct
    10081 gcgtccgtgg gatttcgcgg caggcgagct gctggttcgt gaagcgggcg gcatcgtcag
    10141 cgacttcacc ggtggtcata actatatgct gaccggtaac atcgttgctg gtaacccgcg
    10201 tgttgtgaaa gccatactgg cgaacatgcg tgacgagtta agcgacgctc tgaagcatta
    10261 atgactcagg cgggtgatat cactcacccg ccctcgcctt tcaggcgcta ttccgaaata
    10321 cttcctcacc gctttacttt ctttcgtcac tctcccacca tctttccccg attaatggat
    10381 gataaagaag tcgcaaggaa aggttatggc actgccagtg aacaaacgcg ttcccaaaat
    10441 tctgtttatt ctctttgttg ttgccttctg cgtttattta gtgccgcgcg ttgccatcaa
    10501 cttcttctat tatcctgacg ataaaattta cggtcccgac ccctggtcgg cggaatccgt
    10561 cgaatttacg gctaaggacg gtactcgtct gcaaggctgg tttattcctt cttcgacggg
    10621 ccctgctgac aacgccatcg caaccatcat tcatgctcac ggcaatgccg gaaatatgtc
    10681 cgcccactgg ccgctggtca gttggttacc tgagcgtaat ttcaacgttt ttatgtttga
    10741 ttatcgcggg tttggtaaat caaaaggcac tccgtcccag gccggattgc tggacgatac
    10801 gcaaagcgcc atcaatgtgg tgcgccatcg cagtgatgta aacccacaac agctggtgct
    10861 gttcgggcag agtattggcg gggcgaatat tcttgctgtt attggtcagg gtgatcgtga
    10921 aggcatacgt gcggtgatcc tcgactccac atttgcctct tatgcaacca tcgccaacca
    10981 gatgatcccc ggcagtggct acttacttga tgagagttac agcggcgaaa attacatcgc
    11041 cagcgtcagc ccgatcccgc ttttactcat tcacggtaaa gccgatcacg ttatcccatg
    11101 gcagcacagc gaaaagttgt atagcctggc aaaagagcca aaacggttga tcctaatccc
    11161 ggatggcgaa cacattgatg ctttttccga tcgtcacggc aatgtttatc gcgaacagat
    11221 ggtggacttt atccttagcg cgttgaatcc gcagaactaa cccatgatcg ctagcacgat
    11281 aatcattcac aaaaccacct taagacatgc taatccactg gtcagaacag tttaaaatga
    11341 gaaaaattct gtgacgcttg ccaacatttc tgatgattag cattcccttc gccatttccc
    11401 tgagcaaact ttagctattc ttatcaatta tgcttatggg agatctacag atgatgccta
    11461 cgcttgctcc accatctgtc ctttcggctc cccagcgccg ctgccagatc ttgctgacgc
    11521 tctttcagcc ggggttgacc gccaccatgg caaccttcag cgagcttaat ggtgtggatg
    11581 atgatattgc cagtcttgat atcagcgaaa caggacggga gatcctgcgc tatcatcaac
    11641 tcacactgac gactggttat gacggtagct accgggttga aggtacagtg cttaaccaac
    11701 gtttgtgttt atttcactgg ctacgacgtg gtttccgtct gtgtccgtca tttattacca
    11761 gccaattcac ccccgccctg aagagtgaac tgaagcggcg cggaattgcg cgtaactttt
    11821 acgacgatac caatctacaa gcgttagtga atctctgctc ccgacggctg caaaaacgct
    11881 ttgaatcgcg cgatattcat ttcctgtgtc tgtatctgca atattgtttg ctgcagcacc
    11941 acgctggaat tacgcctcag tttaatccgc tccaacgtcg ctgggcggaa tcctgccttg
    12001 aatttcaggt agcgcaggaa attggacgcc actggcagcg tcgggcgctc cagcatgtac
    12061 cgcctgatga gccactgttt atggcattac ttttttccat gttgcgggtt cccgatccac
    12121 tgcgggatgc gcatcagcgg gacagacaat tgcgtcagtc tatcaaacgt ctggtaaacc
    12181 attttcgtga gctgggaaat gttcgttttt atgatgaaca ggggttatgt gatcagcttt
    12241 atatccacct cgcccaggcg ttaaatcgca gtttgtttgc catcggtatt gataataccc
    12301 tgccggaaga gttcgccaga ctgtatccac gcctggtacg caccacccgc gcggcgctgg
    12361 ccggatttga aagtgaatac ggcgtccatc tttctgatga ggaaagtggt ctggtcgcgg
    12421 tgattttcgg tgcctggcta atgcaggaaa acgatctgca tgaaaaacag attattctac
    12481 tgaccgggaa tgatagcgag cgagaagcgc agattgagca gcagttacgc gaactaacgt
    12541 tactgccgct caacattaag catatgtcgg taaaggcatt tttgcagaca ggcgctccgc
    12601 gtggcgcggc actgattatt gcgccttata ccatgccgtt accgctcttt tcaccaccgc
    12661 tgatctatac ggacctgacg ctgacaacac atcaacagga gcagatccgc aaaatgctcg
    12721 aatcagcatg agggaacaac tttcgggcgc aaaaacattg ccggaagcgc caccagcgcc
    12781 attacccaga acacgccgtg gcccagatat tgatacagga aaccggcgaa aacggtcatg
    12841 atagcgatac tgccgcccat cgcgacggca gagtaaaccg cctgtaaacg gatgacttcg
    12901 ctaacctggc gagcagcaat ataacgcatg gcggccaggt ggcagaccgt gaaggtgccg
    12961 caatgcagaa tttgcaccac tatcaaccac ggcaacgccg tagttgctcc cataatgccc
    13021 cagcgcacta cgccgcaaat cgccgagatc aacagcatat cgcgagcact acaacggcgg
    13081 aaaagtttat tactcagcgc aaagataatg acttccgcca ccacgcccag cgaccacaaa
    13141 taccccaccg ccgaggccga gtagccagct gcctgccagt aaatggcgct aaaaccgtaa
    13201 taggccgcat gtgccccctg caataaacaa acgcaggcca gaaagcgcca gttctggcga
    13261 accagcgcca accacgcaga ccaaccggtg ctctcctgct ggcggcttgc cccttgtggc
    13321 tgaatcgtcg gacggatgag aaagccgagc agcatggatg ccacgcccaa cgtcaacagc
    13381 acgaggatca cccgataatc aaacatagtg accagtttgc ccgtcagcgc cgagccaatg
    13441 acaaacgcca ccgagcccca cagtcgcact ttgccgtaat caagcgggaa ctgcttttgc
    13501 cacgtattcg ccagtgcatc ggtcaacggt accagcggtg agaaaaagag gttaaagcca
    13561 atcatcacca gcatcagcca cgctacgtgc gcccccgccc agaaggcgac agcaaagaga
    13621 agtggcagca gtgccagcac gcgcaaggcg gaaatcaggc gggaaggatc gctgacgcgg
    13681 ggcgcgatga gcaaactccc gagaaaacgg gcaaccagac ctgcccccaa taacaggccg
    13741 atggtttctg gcgttaaacc aatcccttta agccagacgc tccagaaagg taaaaaaatg
    13801 ccgtaactaa aaaagtatgt gaaatagccg agcgccaacc agcgcgtgga ttgcaaaacc
    13861 atgagtccct cccgtcagga ggcgttagtc tggggttaat cgaatgttgg tgcaagttga
    13921 aaaataagaa tattcgagcc tgcttcccga tgtctgtaac gccagttcac aagcactaaa
    13981 aaactgcgat ccgcgacgca tgttgcaaaa ataatgcgta attatgccgt tacgcttgcc
    14041 aaacgttcct gcacaatggc gatgaaatcg cgtaatgcca gcttcatctc ccctttcttc
    14101 cacgccatca gtaaagcaat ggaaggcaca tttccggcaa tgggacgaaa aacaacctgc
    14161 ccggtattaa aattattcat ataaccgggt atcaaagtga cgcccagccc catgcccacc
    14221 agattcatgg tcaccagaat attcgttgcc acctggacga tatttggctg gctgttttct
    14281 tgcgcaaacc acgctttaac gatcggcgca agcgaaccgg aatacgccgg atcggtactg
    14341 acgaaattca cgccatccag ttgcgcggcg gtgatctctt tttcatgtgc taaagggtga
    14401 tcaaccggta acaccaccac taatggttcg tcaaaaagct ccagataatc aatctccggg
    14461 ctataaacag gatggcgcat taagccgacg tcgagttcac cacggcgaat tttttcctcc
    14521 tgttgcgtgg tgattaaact caccagctca atcaaggtgt ctggctgtct gagacgaaac
    14581 atcggtaata cttttggcaa taaattcact tccgccgatg gcacaaagcc aatggttaat
    14641 tgtctgtctt cctgaacaat tttccgcgcc cgtaatttgg cattttccgc ttgttcgagg
    14701 attgccagcg cgtcctggag aaaacattct cccgccgccg tcaacgcgac tttgcgctta
    14761 tccctcacca gtaacggaac accgacacag ttttcaagat cgcggatctg gctgcttaac
    14821 gaaggctgtg aggtatgcag tttttccgcc gcacgggtaa agttcagtgc ctgcgccact
    14881 gcgacgaaat agcgtaaatg ccgtagttcc atcaccttcc ccttgttatc gaaaaaacgt
    14941 ctcagctggt agaaattaac tatttcacat attagcaacc aaccagcaac atccttatgg
    15001 cacaaaaata gaaggtcaat acatcttatc tttcaggatt aaaaaatatg accacaccct
    15061 cagatttgaa catttaccaa ctgattgaca cccaaaacgg tcgggtcact ccgcgtattt
    15121 ataccgaccc ggacatttac caactggagc ttgagcgtat tttcggtcgt tgctggctat
    15181 ttctcgccca cgaaagccag atcccaaaac ccggtgattt ctttaacacc tacatgggag
    15241 aagatgcagt tgtcgtggtg cgccagaaag acggcagcat caaggcgttt ctcaaccaat
    15301 gccgccaccg ggccatgcgt gtgagttatg cagattgtgg caacagtcgc gcctttacct
    15361 gcccgtatca cggctggtct tatggcatta acggcgagtt gatcgatgta ccgctggaac
    15421 ctcgcgccta cccacaaggg ttgtgtaaat cccactgggg gctaaacgaa gttccttgtg
    15481 tggagagtta taaaggactg atttttggca actgggatac cagcgcaccg ggcctgcatg
    15541 attacctggg tgacattgcc tggtatctgg atggcatgct ggatcgtcgc gaaggcggca
    15601 ccgaaattgt cggcggcgta caaaagtggg tgatcaactg taactggaaa ttcccggcag
    15661 agcagttcgc cagtgaccag tatcatgctc tgttcagcca tgcttctgcc gttcaggtat
    15721 taggggcgaa agatgatggc agcgataagc gcctcggtga tggacaaacc gcccgcccgg
    15781 tgtgggaaac cgccaaagat gcgctgcaat ttggtcagga cggtcacggt agcggtttct
    15841 tctttactga aaaaccggat gctaatgtct gggtcgatgg cgcagtttca agctattacc
    15901 gcgaaaccta tgccgaagca gaacaacgtt taggtgaagt tcgcgccctg cgcctggcgg
    15961 gtcataacaa tatattcccc acgctttcat ggctaaacgg cactgccaca ctccgcgtct
    16021 ggcatccgcg cggccctgat caagttgaag tgtgggcgtt ctgtattact gacaaagccg
    16081 cctccgatga agttaaagcc gcttttgaaa acagcgccac tcgtgctttt ggtcctgctg
    16141 gttttctcga gcaggatgac tcggagaact ggtgtgaaat ccagaaattg cttaaaggcc
    16201 accgcgcccg caacagcaaa ctgtgtctgg aaatggggct tggtcaggaa aagcgccgcg
    16261 acgacggcat tcctggcatt actaactata tcttttcaga aactgccgct cgtggaatgt
    16321 accaacgctg ggccgatctt ctgagtagcg aaagctggca ggaagtgctc gataaaaccg
    16381 ccgcttacca gcaggaggtg atgaaatgag tgcgcaagtt tcactggagt tacatcaccg
    16441 cattagccag tttctctttc acgaagccag tctgctggat gactggaaat ttcgtgactg
    16501 gttggcacag ctcgacgaag agattcgtta caccatgcgc accacggtta acgcgcaaac
    16561 acgcgaccgt cgcaaaggcg tccagccgcc gacgacctgg attttcaatg acaccaaaga
    16621 ccagctggag cggcgaatcg cccgtctgga aacgggcatg gcctgggcag aagagccgcc
    16681 gtcacgcact cgtcacttaa tcagcaactg ccaggtaagc gaaaccgaca tcccaaacgt
    16741 atttgccgtg agggtgaatt atctacttta tcgggcacaa aaagagcgcg atgaaacatt
    16801 ctatgttgga acgcgtttcg acaaagttcg ccgtctggaa gatgacaact ggcgcttgct
    16861 ggaacgggat atcgtcctgg atcaagcggt gatcacttcc cataacctga gtgtactgtt
    16921 ctgatgaatc gaatttatgc gtgtcccgtt gcggatgtgc cggagggtga ggctctccgg
    16981 atagatacct cgcccgtcat cgccttgttc aacgtgggcg gcgagtttta tgccattaac
    17041 gatcgttgca gccatggtaa tgcgtcgatg tcagaagggt atctggaaga tgacgccacg
    17101 gtggagtgcc cgctacacgc cgccagtttt tgcctgaaaa cggggaaagc gttatgcctg
    17161 cccgccaccg atccgctcac cacttatcca gtacacgttg aaggtggtga cattttcatc
    17221 gacttaccgg aggcgcagcc atgagcgatc tgcataacga gtccattttt attaccggcg
    17281 gcggatcggg attagggctg gcgctggtcg agcgatttat cgaagaaggc gcgcaagttg
    17341 ccacgctgga actgtcggcg gcaaaagtcg ccagtctgcg tcagcgattt ggcgaacata
    17401 ttctggcggt ggaaggtaac gtgacctgtt atgccgatta tcaacgcgcg gtcgatcaga
    17461 tcctgactcg ttccggcaag ctggattgtt ttatcggcaa tgcaggcatc tgggatcaca
    17521 atgcctcact ggttaatact cccgcagaga cgctcgaaac cggcttccac gagctgttta
    17581 acgtcaacgt actcggttac ctgctgggcg caaaagcctg cgctccggcg ttaatcgcca
    17641 gtgaaggcag catgattttc acactgtcaa atgccgcctg gtatcctggc ggcggtggcc
    17701 cgctgtacac caccagtaaa catgccgcaa ccggacttat tcgccaactg gcttatgaac
    17761 tggcaccgaa agtgcgggtg aatggcgtcg gcccgtgtgg tatggccagc gacctgcgcg
    17821 gcccacaggc gctcgggcaa agtgaaacct cgataatgca gtctctgacg ccggagaaaa
    17881 ttgccgccat tttaccgctg caatttttcc cgcaaccggc ggattttacg gggccgtatg
    17941 tgatgttggc atcgcggcgc aataatcgcg cattaagcgg tgtgatgatc aacgctgatg
    18001 cgggtttagc gattcgcggc attcgccacg tagcggctgg gctggatctt taaggaagca
    18061 cgatgaaaga aaaaacgatc attattgtcg gtggcgggca agcggcggca atggctgcgg
    18121 cctcgctacg ccagcaaggg ttcaccggtg agctgcatct gttttccgat gagcgacatc
    18181 ttccttatga acgacctcca ctctcgaaat ccatgttgct ggaagattcc ccgcagttac
    18241 agcaggtgtt acccgctaac tggtggcagg aaaacaatgt tcatctgcat tccggtgtaa
    18301 ccatcaaaac gctgggtcgc gacacacgag agttagtgtt aaccaacggc gaaagctggc
    18361 actgggatca gctttttata gcaaccggcg cggcagctcg accgctgccg ttgcttgatg
    18421 cactgggaga acgctgcttt accctacacc atgccggtga tgccgccaga ctgcgagaag
    18481 ttctgcagcc cgaacggtca gtcgtgattg tcggtgccgg aactattggt ctggaactgg
    18541 ctgccagcgc cacgcagcgc agcgcagcgc agcgcagatg taaggtgaca gtgattgaac
    18601 tggcggcaac cgtcatgggc cgtaatgcac caccgcccgt gcaacgctat cttttacagc
    18661 gccatcagca ggctggtgtg cgcattctgc tcaataatgc cattgaacat gtggtcgatg
    18721 gtgaaaaagt agaactgacg ctgcaaagtg gggaaacgct tcaggctgat gtggtgattt
    18781 acggtattgg tatcagcgcc aacgagcaac tggctcgcga ggccaacctt gatactgcca
    18841 atggcattgt cattgatgag gcttgccgca cctgcgatcc cgcgatcttt gccggtggcg
    18901 atgtggcaat cactcgtctt gataatggtg cactacaccg ctgcgaaagc tgggaaaacg
    18961 ccaataacca ggcgcaaatt gccgctgccg caatgttagg gctaccgcta ccgctactgc
    19021 cgccgccgtg gttctggagc gatcagtaca gtgataactt acagtttatt ggcgatatgc
    19081 gtggcgatga ctggctttgt cgtggcaacc cggaaactca gaaggcgatt tggtttaatc
    19141 tgcaaaacgg cgtgcttatc ggtgcggtca cgctgaatca gggccgtgag attcgcccaa
    19201 ttcgcaaatg gatccagagc ggcaaaacgt ttgatgcgaa actgctgata gatgagaaca
    19261 tcgcgcttaa atcactgtaa tcaggataat tagcgaatat ctcaatgcct ggggcgtggc
    19321 gaggtgcaag agtgtgtatt acgtttaaat cacattatct tgcaaaggga ttggttatga
    19381 acacactacg ttattttgat tttggagctg cccgccccgt tttgttatta attgcccgta
    19441 tcgccgtggt cttaattttc attatttttg gttttcccaa aatgatgggc tttgacggta
    19501 cggtccaata tatggcctcg ttgggcgcgc caatgccgat gctggcagcg attattgcgg
    19561 tagttatgga agtgcccgcc gcgatattaa tcgtgcttgg ctttttcacc cgtccgctgg
    19621 cggtgctgtt tattttctac acgctgggta cggcggtgat tggtcaccat tactgggata
    19681 tgaccggcga tgcggttggg ccaaatatga ttaatttctg gaagaatgtc agtatcgctg
    19741 gcgcgttctt gctattggca attaccggac cgggggcaat ttctctcgat cggcgttagg
    19801 aaagatgccg gatgcggcgt gaacgcctta tccggcattt tataaattac agccactcta
    19861 cccgcaacga catctctgag gttgttgatt cccctggtgc tagcgcaatg agatcacccc
    19921 cttccgggcg atgatgctca tccggcgcat ggctcatcgg ttccagacag aagaaatcaa
    19981 acgcatatcc tttatcaaac gcagggtcgg aaacaaagat gaaataacac ggtgcaggtg
    20041 gcgtcgtttc catgatgatg gcataacctt cctgcggctg ctcgatgcgg gcctgaccat
    20101 tccatccggc aaaaccattg ttcacccact gacgcggcaa cggcgcgagc tggttaaaat
    20161 ccagttcctg cggtagctgc tcgcaaaact cacccgccag ccactgctcc cgttccagcc
    20221 agtaaccgct cgcctgcgcc tgaatccgcg tttgtggtga caacggaaaa taaggatgcc
    20281 agccggtacc aaatggcagc gtctctgccc cttgattggt gacagagagc gtcaccgtca
    20341 gcgtatccgc cgttaaatga aacgcctgac ttacccgata gtgatagaca ccgctgcgat
    20401 gttcatacac cagacacaaa ctatcatcgc tgtgcgagac acattgccac tcgcccagcc
    20461 agccatcgcc gtgtagatag tgcgcatccc actcaacatt cggttgcagt tgatactcac
    20521 gcccctgcca gacaaaacgg ttgccgctca cccggtttgc aaatggcacc agcggaaaac
    20581 atgaggcatc ggttgccaca ccgctttttt taccaggacg taagagcggc gtcgtatcgc
    20641 gccagaagcc ttcgattacg ccgccctggt cagaaacgtc cagctttagc gacccgtggg
    20701 ataaggtata gatggtcatc cgctaactcc ttaatccggg aagttaatca caactttccc
    20761 gcatttgccg ctcgccatca gcacataggc atcacctgcc tgttctagcg agaagcgatg
    20821 ggtaatggcg ttacgcggcc acagcttcca gtcagtcaaa tcgtgggcgc atttttccat
    20881 atggaacaga ctggtcaccc aggagccgat aatccgccgt tgatggtgca tcagatcagc
    20941 gctgacctcg aattccactt ttccggtttc accaatgtaa accacccgcc cccagtcagc
    21001 agtggattgc agtgccagca agcgacctgc ggcattaccg gaacaatcga gcgcaacatc
    21061 cgcgccaccg tgggtaagtt cagcaattat ctgcggcaga ccttcggtgg ttgctaaata
    21121 gccgtgatcc atccccccta actgttttgc catcgccaga cgttccggca gcatatcaac
    21181 gccgatgatc cgttttgcac cgcgaccttt cgccagcatc atcgccatca tgccgaccgg
    21241 ccccagaccg accaccagca cgttatcgct gccggaaact tcgccgcgca aaatcccttc
    21301 ataggccgtg ccaacgccgc aactgataaa cgcaccatct tcgtagctca gcgcatccgg
    21361 caggaggatc agatcttttt cttccgccag cagatattcg gcatgaccgc cgtcacgctg
    21421 ccagccgtaa gccgcttttc ctttgccagt acaagagata ggaaagccgc ggcggcagtt
    21481 cgggcaaaaa ccacagccag aaatgtgata caccagcacg cggtcgccct ctttaaaatg
    21541 gcggcagcct tgccccatcg ccacaatctg cccgcacggt tcatgaccgt tgataaatcc
    21601 ctggtataac ggtttatcgg gtgccgccgc cgtggcacgg tgttgatgat agatatagtg
    21661 gacatcgctt ccgcaaatcc cggaggattt cattttgatc agtacctggt taatccccgg
    21721 cgtcggcacc gcaacttccc gcagatcgac ggttgaattt cctagtaaat aagcagccag
    21781 catcgttttc ataaatccct cattaacaat acgattaatt ttcatccctg cagcacccgc
    21841 gccaggggcg ttaaggttta gcgtttcgct ttactgcttc gctgggtcag caagatattc
    21901 gccagcaccg ccaccacgat gatgacgccg cgtaccacct gctggaaaaa ggagttaata
    21961 ccgagcagca ccagaccgtt accgattagc gtaatcacca gcacaccaag caatgtaccg
    22021 aacagggaac cgcgaccgcc ggaaagtgcc gtaccgccga ccacgaccgc ggcgatgacg
    22081 tcaaactcca gaccgtttgc ggcacctgcg ttaccggaac cgaggcgtgc cgccaacaaa
    22141 atgccggtca ccgccgctaa taatcccgaa agggtaaaga taagaatgcg cactcgacga
    22201 acgttaatac cgcacaactg cgccgccgtg gcattaccgc caacggcaaa aaccgagcgc
    22261 ccgaaggcgg tttttcggct aatgaacaca aacagcgcaa acaacaccat catgatcagc
    22321 gcggatactg gcacaccgag aaattgtccg cccagccagt ccagcacctc gttttcgtca
    22381 atcggcactg gcagtgcgtt ggtcataaac agccccattc cgcgcagagc gctccacagc
    22441 cccagtgtgg caacgaaact tggtacgtta aacacgccgc gcagcacccc ggcgagcgtt
    22501 cccatcagcg cgcctaacag caacaccagc agacaagcaa tcgccaacgg aacttcaaat
    22561 tgcagcaaaa atgccaggca caccgagaca aaagccacca tcggcccaac gctgacatca
    22621 atttcaccgg agataataat cagcgtcatc gcccaggcgg caatcccaat ggtggcggca
    22681 tcgcgcagca cgttcatctg gttattcaat gagataaagc caggcgcgtt cagggagaag
    22741 accagataaa gaatggcaat caccaccagc aaaccgatct cattaatatg gcgactgaca
    22801 aattgtttga gcgagacgct cttgccctgc ggcaatggta atgacgaagc atacatcttc
    22861 aggttcctcg gtaaaattca tcagtgcaca gacagaatgg cggacatcag ctcatccaca
    22921 ttgaccggcg agtgaaactc ctgcgagaac gtgccgtgct gtaataacag gatgcggtca
    22981 cacaccagcg gcaattcctc cacttcactg gagataaaca ccacgctttt tccttcggca
    23041 gccagctcac ggacaatacg gtaaatctgc tgtttggctt cgatatcgac gccgcgcgtt
    23101 ggctcgtcga gcaacaaaat ctggctggcg gcatagaccc aacgaccgat caccactttt
    23161 tgctgattgc caccagaaag cgtgccgatg ggtgtttcgc tactggcagc cttgaccgtc
    23221 atccgctgca tcacctcttc ggtcaggcgg cggatggtgg accattgcag cacaccgttg
    23281 gtgctgattt tttgccgatt ggtcagcact gtattttcgt caacgcctag ccagggaatg
    23341 atccccgctt ctttgcggtt ttctggcgta tagccaatgc cgcgtttcag catgtcgccg
    23401 taatcggggc gcatgatttt ctcgccgttg ataacaattt cgccctgttc atacgtctcc
    23461 agcccaacaa tcgccttcag caattcactg cgccctgccc ccagcaggcc agcaatgcca
    23521 agcacttcgc cacgacgtag cgtaaagctg atatcctcca gcttgggctt atggcgtaac
    23581 gcacggactt ccagtacggc ctgatccaca atttcctgag gggcaaccgg cgcaatatca
    23641 acgtgatcac gccctagcat tagcgacaca atatgatgcg tggaggtgtt ttcgagcatc
    23701 acatcgcccg ccacctgacc atcgcgcata acggtggcat aggaggcaat gcggcggatt
    23761 tcttccatcc ggtggctgac ataaatcacc gccacgccca gtgccgacat ctttttcacc
    23821 gcactgatca ccagttcaac ttctgcactg gcaagcgagc tggtgggttc atcaagaatg
    23881 accacgcgcg gctcgccctt catcacccgt gcaatttcca ccagttgctt ttgcgccggg
    23941 cttagcgttg aaacaagttg ttcaggacta acgtcaacgc ccagcgcctg taagcaacgt
    24001 tgggcatcct gcgccatttg caggtaatca atcatgccgt tgcggcgagg ccattgacca
    24061 aggcagaggt tttctgccac cgtcagccct tccaccagac ttaattcctg ataaaccgca
    24121 cgaaccccca gttcagcggc acggcgagtc aacgtagctt cgtcaccttc cagtcgcgtc
    24181 tcgccaatcc agatatcacc gctatccggg cgttcgctgc cggtaagcat tcgaatgaga
    24241 gtcgatttgc ccgcgccgtt tttacctaac agcgcacgaa cttcgccttt attgagcgtg
    24301 aagttaacgt tatccagcgc aacgacgccg gggtaacgct tatttcctgc caccactttt
    24361 gctaccggga ctgcctctgt tgccgtgaac atagggacct ctgcgaatca gcgattaggg
    24421 cagaccatca acgtgcgttg ccagccactg tttaccatct tccgttttgg tatagagatc
    24481 gattggcacc tgaatcactt tttcaccgtc ggcttgttta ttgataacct tcaatgtttg
    24541 cgcgaaaaca gcattgccca ttttcttacc ggaaatatcc actaccgctt tcagcacctg
    24601 attgttttcc agctcctgag caatttcggt tgtcatatcc gaaccgaaaa cagcaatttt
    24661 tccggcctga ttttgattac gtaccgcttt taccgcgccg agtgtcgcac cgcccgactc
    24721 ccccataatg gcgttgagat ccggcgtgga gataatcagt ttttcaccaa cggaaatcgc
    24781 tttatctaaa acagtccctt cctggttagc gacaatttgc gcgccgggaa cgcgggattt
    24841 taatacttct tcaaatcctt tacgtcgctg cacacaaact tcaaaggctt cgcaattgat
    24901 gacggcaatt ttcggctggt caattttatt ggcaataaaa taatcggcgg cagcgttacc
    24961 cagtttttac caaattccag tggatcgccg accagatacg ccgagacata tttatcgaca
    25021 cccttttgat taatacaggt gttgtagcaa atcaccggaa tacccgcttc actggcgcga
    25081 cgaacggtac ggctactgcc attttcagac actgccgata aaataatggc atcgacatta
    25141 cgcgccacga gggtatcaac aaaggtactt tctttcgaaa tatccccctg ggcgttagtt
    25201 tcaattaact gtacctgtac tgaagaatct tttgccgcat cctgaacgcc ctgacgcact
    25261 ccagcgtaat atccctgggt atcaaggtat attgcaccaa tggtcatttc tttatccgca
    25321 gccctggcaa atagtgcgct acctaatagc gtagccatta ataatagatt acgggttgtt
    25381 ctcatttttt taggcatagt gcctcctgta gggtttttat taacaacggc ttattctaat
    25441 tattttgtga tgagcggcag cgcgtgccgc tcaaatatta cagttaatag ggtttagtga
    25501 acgtagttaa aaataaaagg catcacagtg gtgaataatg ccgctttcgg ccaggccgga
    25561 ttaatcgccg tcaattcagc gcgagcagac tcatattgcg caacctcccc gagtcccgcg
    25621 gacgccagca tcgccaccat caggcatttt tctttatgct gctgttgtaa atcgccatac
    25681 agcgataaca ggtcaggttg tgaaacagca aagaaatccg cctcaatact ggttttcgcc
    25741 atctcttgcg cccactgttt catttcacta aacagttgct gtgcggtttg ctgttcaccc
    25801 agcagtcgca gcgccattcc ttgccagaag agataatcaa ccggctgatc gttgtaataa
    25861 ctgtggatgt taatggtgcg atcgccggtc gccgccagac gtaaacaacg catcgcttca
    25921 gtttcatcgc cctgcgcgtt ggcgcatatc gcctgccaga accagatgtc gttatcagtt
    25981 tgccccggta aacggccttc gcttaaattc tccggataat acagcgcggc atgaagcagt
    26041 tcgctggcct gctgcggctc tctggcatca agatgctgcc aggcgcgtaa taactggttg
    26101 aggataaact gactggtgac cttcccttcc ccgccttccc acgggtggaa tttacgcgtg
    26161 gcgagaatgt ccgccgcttt gtctgcctga cccgtgagat gccacaaatt gagcagttct
    26221 gcggtcatgt cgtcgcgttt cagcgcaatt tccagattat tttccagacg cgccagtcgt
    26281 ttctccggtg tggctccact taacttatcc agcaaatccc gttcgaaaag cagacgtgca
    26341 tcctgcggcg caagctgata agcattatca agataacgcg cggccagctc ataatcgtgt
    26401 ttcttattcc acgcatggat cgctaacccg cgccagccgt cggcaaactc cggcgacatc
    26461 tctacgcaac gttgccataa ggcaatggct ttgttgtagc tacgtttgtt gtagtagaag
    26521 caggccagta aatggcgagc aaaccaacac tcttcaatac tctccagcgc cgccacttct
    26581 tccagcgtat tcgggaaacg gacaaactgc gggaagacat caatggcttt tgcgaccagt
    26641 tcgccacgtt cggctttcgg cagcaagctg gcttgcaggt aaagcggcag cgtgcgctgg
    26701 cagtccagtg cgttcagcat ctctgccgcc agggtgggca ttccccagtt aatcagttgc
    26761 ccggcggtca tcagagcgtt aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgagg
    26821 gctgattcgc tacgaccatc gaaccagttc agccaccaca gagtggcgtt cagcggataa
    26881 tcacgcagca gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact taacaccagc
    26941 agcagattat gcaagcaaag cacttcctga ttggttgggc aggcacgaag actttgttgg
    27001 caaaaatcca gacccgcgtc gaagttatca ttacgcgccg ccagtcgtgc cagaccataa
    27061 tagccgcctg ctttactatt gccgctccag accgcacgcc agaaatcctc ttcggcttgt
    27121 tgatattgtc cctgacgttc gtaagcactg gcgcgaatca aacttgcttg tccacactgc
    27181 ggttttttgt tcagcgcatg tgcacgtttc agcgcctgac tggcatacgc caccgcttgc
    27241 gggaaatctg cgcggttata ttccagcatc gccagcgcca ggttacagcg ataatccagc
    27301 gggtccagcg ccacgccgcg caggtagtaa tcgaacggtg aacggctggc gtgatgatat
    27361 tgctccagat gctgaccgat aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc
    27421 agtggcgctt tggcgacgtc cggcaacggc aactcctgcg gctgatgttc ctgataactc
    27481 agtacaatat tgccgtcggc atcggagagt tcaatggtca gtctgtcagg attgataccg
    27541 tgcaacacgc cctggatggc ggtcgctggt gtcagggcca cggcatcatc gagcaacgcg
    27601 ttgcatttgc cgatttcgcg gatcgccaga cggtatccgt tcaacggaga gatggcatac
    27661 agcccccact caatcccccg ctcactacgc tggagtttta tcaccgcatc gcgggaggca
    27721 ttttgcacca tgcccagaga atgataaggc aggaaatact gctcgaaacg cttctcttcg
    27781 taagcatcaa gccaggtaaa atcaggctgg ttatcggcaa aaataccggt catcagttcg
    27841 atatacgggc cgttattgtc ggtcagactc ttatcccacg cctggccaaa ttcactgtgt
    27901 ccccaactcc actgtttttt acctggcgca atatggtggt tggcaacgtg cagcaaaccg
    27961 ccatcttcat cgtgacacca cgcgccgaca aaatcgtact gcgatttttc ggccatatat
    28021 gaggttggaa caggcacatt tttatagcga gaaatgtcca ctccggcgga gtagtccact
    28081 ttgtagtaag tgccggtggc gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac
    28141 accgcagtca catccggtgg gaaaacgctc tgatgccctt cacccccttt cactgccggg
    28201 ttgacccacc acaagaaatg acgcggcgtg gcgttgccgt tatagacgcg gctggcgatt
    28261 tccagtgccg cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa accatgcatc
    28321 ggctccgttt cgccgaccca caccgtctgt gcaccgtctt catgggcttc gagggtgaaa
    28381 tcaacgggca taaaggtggt cgggcgatgg tgttgcggcc agttaaactc aatcccgccg
    28441 gagatccacg gtcccagcat ccccaccagc gcaggtttaa tgacttcatt gtgatagaca
    28501 aaatcgcgct gtttcacttt atcccatgcg cgatgcaccc gaccgcccag ttccggcagg
    28561 atcatcactt tgatgtagtc gttttccagc cacaccgcct gctaggattt cagggttttc
    28621 tgctcgctca gcgtatcggt cacgccgtag ggataaaccg cgccggacga tccctgataa
    28681 acgcgatttt ccaggaacat gggatgtata tcctgcggcc cggtttcata ggtcgggatc
    28741 tcaacgcgct cttgccacac ttttactgga gtcatggtgc cctcaatatt aacaagacat
    28801 actgaattaa aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa
    28861 tcacgcaatt aactaaacaa ggtttagtga agatgagagc ctgcattaac aatcaacaga
    28921 ttcgccacca taacaaatgc gtgattctgg aactgctgta ccggcaaaag cgcgccaata
    28981 aatcaacgct ggcccggctg gcgcaaattt cgattccggc agtcagtaat attttgcagg
    29041 aactggaaag cgaaaaacgg gtggtgaata ttgacgatga aagccagacg cgcgggcata
    29101 gtagcggtac atggctgatt gcgccggaag gtgactggac gctgtgcctg aacgtgacgc
    29161 ccaccagtat tgagtgtcag gttgctaatg cttgtttaag tccgaaaggc gaatttgagt
    29221 atttacagat tgatgtaccg acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc
    29281 atcgccaccg taaattgtgg ccggaccgca ccatcaatct ggcgctggca atccacgggc
    29341 aggttgatcc ggtgaccggc gtatcgcaaa ccatgccgca agcgccgtgg acaacgccag
    29401 ttgaggtgaa gtatctgctg gaagagaagc tgggcattcg ggtgctggtc gataatgact
    29461 gcgtgatgct ggcgctggcg gagaaatggc aaaataattc gcaggaacgg gatttctgcg
    29521 tgatcaacgt tgattacggc attggctcgt cgttcgtgat taacgagcaa atttatcgcg
    29581 gcagtttgta tggtagcgga cagattggtc acaccatcgt taatccggat ggagtcgtct
    29641 gcgactgtgg acgttacggc agcctggaaa ccgtcgcctc gttaagtgca ttaaaaaaac
    29701 aggcgcgggt atggctaaaa tcacaaccgg ttaatactca gcttgagcct gaaaaactga
    29761 caaccgcgca gttaatcgct gcctggcaga gtggagaacc gtggatcacc agctgggttg
    29821 atcgctctgc caatgccatt ggtttgagtc tgtataactt cctcaacatc ctcaatatta
    29881 atcagatttg gttgtacggt cgcagttgtg cctttggtga gaactggctt aatactatta
    29941 ttcgccagac aggatttaac ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc
    30001 aaattggctt cgggcaatta agccgcgcac aacaggtgct gggaattggc tatttgtatg
    30061 ttgaggcgca gttacgacag atttgatggc gcgataacgt agaaaggctt cccgaaggaa
    30121 gccttgatga tcataaacga aaaattgcct gatgcgctac gcttatcagg cctacacgga
    30181 gattgcaata tattgaattt gcaagatttt gtaggcctga gaaggtgttc atgccgcatc
    30241 caacatgaac aacgagcaca ttgtcagcaa atcaccgttt cgcttatgcg taaaccgggt
    30301 aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacggct tcatcattga
    30361 tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttcggct tctttaaagc
    30421 cgcgacgggt aatcgccgga gtacctacac gaataccgga ggtcacaaac gggctcttcg
    30481 gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga gcggcgtctg
    30541 cttctttacc ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg ttatcagtgc
    30601 cgccggaaac cactttgtag ccgcgctcga ggaacacttc taccatcgct ttagcgtttt
    30661 tcgcgacctg ctgctggtaa gttttgaact caggctccat cgcttctttc agagcaaccg
    30721 ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa acggcagagt
    30781 tcagtttttt gtacagctct tcgctaccac ctttcgccaa gatcaggccg ccgcgcggac
    30841 ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc gggttcgggt
    30901 agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacaga taagcaccga
    30961 tgctgtcagc gatttcacgc attttcgccc agtcaaccac gccggaatat gcagagaaac
    31021 caccgataat cattttcggc ttgtgttctt tggcttgttt ttccagatcg gcgtaatcga
    31081 tatgaccggt agcatcgata ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa
    31141 ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt catacccaga acggtatcac
    31201 ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag tgcggctgga
    31261 cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt tgttcaacga
    31321 tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca gcatatttgt
    31381 tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga
    31441 tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc cacagttcgg
    31501 catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga ctcagctaac
    31561 aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag tataaccgaa
    31621 tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc ttcaggctac
    31681 gcaaggcttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc aggtttgtga
    31741 tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca tttacattgc
    31801 agggctattt tttataagat gcatttgaga tacatcaatt aagatgcaaa aaaggaagac
    31861 catatgcttg acgctcaaac catcgctaca gtaaaagcca ccatcccttt actggtggaa
    31921 acggggccaa agttaaccgc ccatttctac gaccgtatgt ttactcataa cccagaactc
    31981 aaagaaattt ttaacatgag taaccagcgt aatggcgatc aacgtgaagc cctgtttaac
    32041 gctattgccg cctacgccag taatattgaa aacctgcctg cgctgctgcc agcggtagaa
    32101 aaaatcgcgc agaagcacac cagcttccag atcaaaccgg aacagtacaa catcgtcggt
    32161 gaacacctgt tggcaacgct ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg
    32221 tggggtaaag cctatggtgt actggctaat gtatttatca atcgcgaggc ggaaatctat
    32281 aacgaaaacg ccagcaaagc cggtggttgg gaaggtactc gcgatttccg cattgtggct
    32341 aaaacaccgc gcagcgcgct tatcaccagc ttcgaactgg agccggtcga cggtggcgca
    32401 gtggcagaat accgtccggg gcaatatctc ggcgtctggc tgaagccgga aggtttcccg
    32461 catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg ctatcgtatc
    32521 gcggtgaaac gcgaagaggg tgggcaggta tccaactggt tgcacaatca cgccaatgtt
    32581 ggcgatgtcg tgaaactggt cgctccggca ggtgatttct ttatggctgt cgcagatgac
    32641 acaccagtga cgttaatctc taccggtgtt ggtcaaacgc caatgctggc aatgctcgac
    32701 acgctggcaa aagcaggcca cacagcacaa gtgaactggt tccatgcggc agaaaatggc
    32761 gaagttcacg cctttgctga tgaagttaag gaactggggc agtcactgcc gcgctttacc
    32821 gcgcacacct ggtatcgtca gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc
    32881 gaaggtctga tggatttgag caaactggaa ggtgcgttca gcgatccgac aatgcagttc
    32941 tatctctgcg gcccggttgg cttcatgcag tttaccgcga aacagttagt ggatctgggc
    33001 gtgaagcagg aaaacattca ttacgaatgc tttggcccgc ataaggtgct gtaatttgat
    33061 gttgccggat ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct
    33121 tcaccggtac ggatgcgaat gacccgagcc acgtcaaaga cgaagatttt accgtcaccg
    33181 attttgccgg tttgcgccgt gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc
    33241 ggtacgacaa tctcaatttt cactttcggc agaaaatcca ccatatactc cgcgccgcgg
    33301 tacagctcgg tatggccttt ctggcgacca aagcctttca cttcggtcac cgtcatgccg
    33361 gtaataccga cttcggccag tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc
    33421 gcatcaatct ttttcatgct attccttgaa aaggttgcct gtcttttgat ctgctaaacg
    33481 taacacataa cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc
    33541 agtttataaa attctgtccg gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct
    33601 ttggtgattt gcagcagctt acgcaagtag ttgagttcaa aatgattacg tgcctcaaca
    33661 aaggttggca gcgccgtatt ttcaccctcc agcgcctgct ccaccagcgc atcactaatc
    33721 accggagatg aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg gcgcacatta
    33781 cccggccagc tcgcggtcat caggcgtttc atcgcatcgg tagagaacgc gcggacaaac
    33841 ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg ccagtagcgg aatgtcttct
    33901 gtgcgctccg ccagtgccgg aattttcagg ctgacaacgt tgaggcggta atagagatct
    33961 tcgcggaatt ccccgcgcgc catcgctttt gacagatcac ggtgagtggc agaaataatc
    34021 cgcacattga tatcaatatc gcggttactg cccagcgggc gcactttacg ctcctgcaac
    34081 acgcgcagca gtttgacctg taacggcgca ggcatatcgc caatttcatc gagaaatagc
    34141 gttccgcctt ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg
    34201 ccacgcgcat gaccaaacag ctccgactcc agcaattgct cgggcaatgc gccacagtta
    34261 atagcaataa atggtttgct gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc
    34321 tctttcccgg tgccgctctg accgttaatc aaaacgctga cgtctgattg cgccaccagc
    34381 cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg tgacaattgc ctcgcgccag
    34441 cgttcatcgg tggctggcgc ggattgctcc agcgcatcgt caattgcctg atatagcgca
    34501 tctttatcga caggcttggt gaggaaacta aaaacgccct gctgtgttgc agcaacggca
    34561 tcggggatag aaccatgcgc agtaagaata attactggca ttcccggctg cactttctgg
    34621 atttcagcaa atagctgcat accgtccatt tcatccatcc gcaggtcgct gataactaaa
    34681 tctacttttt cgcgattcag tacccgtaat ccttcagcgc cactttccgc cgtgaccaca
    34741 ctgtagcctt cgctggtcag gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg
    34801 accaataata aatgcgcagg tttatggctc atcaggagtg acctcatggg tggatggcgc
    34861 gggcttttca ctctcgtgcg gcgtatccgg cgagaaattt ccagccggtt tgcgggtcga
    34921 gagctggcgt tcaatatcgg tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg
    34981 ctgtagaacg tgatgttgct ggcgcaatgt atccagctcg ctgtcgctgg actgctggag
    35041 tttactgtaa cgttggcgct cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca
    35101 gagttgatac agtgggcgaa cctgtgccgg gatctcggta cttaacgctt caatacgcgc
    35161 gaccagctgg cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt
    35221 aaaggtattt tgccagctgc cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg
    35281 cattaaacga tcagcacaat ccatcgcccg cagccagtaa agcggattgg tttcggttga
    35341 tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa agatagtcag ccagttgata
    35401 aaccggaatt ttttcttctg ctggcgtatc aatggctggc ttattgtgat tctgcacgca
    35461 acccagcaat gccagacatg gcagccctgc cagccacagc cgtcggggca ataatcgttg
    35521 aaaaatgtgt cgcatattca ccagacttaa agcctatccc agtgggcgta attgttgcag
    35581 acagtctgga catggacagc gcggagaaac cggggcgtac atatcgtacg tgaagatttc
    35641 gagcactacc cggggccgaa ctgacaaata aaatagcctg ataggatagg ctgttagcat
    35701 tatttcgtgt ttttcgacga cggtaattca atgcggaaac aaacgtcttg cccgctctcg
    35761 tcgaccagat acagttcccc ttgcatacgg cgaatacaat ccctggcaat gcttaatccc
    35821 agaccgctgc ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag
    35881 atcatggcgc gttcctcttg cggaatgggc gtgcctgtat tgatgacatc aatataaacc
    35941 cgcgcaccat gtaaactgct gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc
    36001 gcattggagt aaagattatc cagtacgctc atcagcagca ttggctccgc caggcaagct
    36061 gttgctttga gatcgacgtc ggtatgcatc attttagccc gtgcgggcag gctatgagca
    36121 gaaaccactg tctccaccag cggtgctaat tcaacattct ccagttccac cgcactgtcc
    36181 gcctgtttac ggttgtaatc aagcagttgt tcgatcagtt tttgcaaatt gcagctgctg
    36241 ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa gcggcccgac aacctgatca
    36301 gccagtaatt cagtgccctc gcgcatactc gccagtggcg tttttaattc atgagataaa
    36361 tgtcttaaaa attgatggcg ttgggattcc agccatgaca ggcgctcact taaccaaaga
    36421 atacgttgcc caaccgagcg taactcgctc ggtccacaga acgaaacgct attgcccaga
    36481 gaacgccctt cccccagacg gttgatcatg cgctcgatat ttttcaccgg cccgataatc
    36541 atccgcgtga aaagtagcac catcaccaga ctcaccagaa atagcaccag cgattgccaa
    36601 ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt gctgcccacg agagaacacc
    36661 actgtgcgcg tggcctgcac catttcggta ttggcactgg caaaggcttc aagacgcgcg
    36721 gcggcggcag catcgggacc actgttgtta cactgaagtt gggccagatt gttcaaatcc
    36781 tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt
    36841 tcgctgtaac gcttgcgctg gctttgataa accttcgcaa gcgtagggtc gtccagcacg
    36901 caatactgac ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc
    36961 cgggcatcaa taagcgtagt gcggttaacc agcgccgcct gatcgctaag cgcattcagg
    37021 ctttgccagg cttgccatgc cagcaccaac aggggtagta gaattagcaa aaatgccagc
    37081 attaccagtt gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt gttactctcg
    37141 tcagacgcga atagcctgat gctaaccgag gggaagttca gatacaacaa agccgggaat
    37201 tacccggctt tgttatggaa taaggcggtg cctaactcga cgtttcgccc gatggttgat
    37261 atagctacgc tgatatcaga agctggacgg caggcacctt gttgtgcgtc attcgaattt
    37321 tatgtagcac gtcccgaagg ggctgacata agtcggtgaa tgagccactg cttactatta
    37381 tgcagtaact gtgccaataa agaaaatagt ttggtaacgt actgattata cgttgctttg
    37441 agggatttat ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag
    37501 acaacttaag ataaacttat tagattgcaa taaaatcaat catttaagtg tcgccagaaa
    37561 gcgacacggt aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg
    37621 gagcttatga tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga
    37681 tgacttaccc caactgctta cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc
    37741 cccagttttc cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca
    37801 tcataatggt gactcgacca ctttcagtcg taaccgccgt aataccgttc ggtgaaccgt
    37861 tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca
    37921 ccagcccttt gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt
    37981 caccatgaga gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagagacg
    38041 ggctttgggt tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtgttacgca
    38101 caaaacgtgg ccacaactca ctccccggga tcagttcacg caggttagac atcatctggc
    38161 aaccgttaca tacccccagg gccagcgttt gcggacgatg gaagaaggtt gcaaactcat
    38221 cgcgtacacg gtcattgaac aggatagact tcgcccaacc ttcaccggca cccagcacat
    38281 caccgtagga gaaacctccg cacgcgacca gggcgtgaaa atcctccagt cccgtacgtc
    38341 cagtcagcag gtcgctcata tgcacgtcga tagcatcaaa gcccccacgt tggaaagctg
    38401 ccgccatttc aacatgcgag ttcacgccct gctcacgcag tacggcaact ttcggacgtg
    38461 cgccagtggc aatatacggt gctgccacat cttcgttgat atcgaacgac agttttacat
    38521 tcaggcccgg atcggcgtcg ttagatttcg cctgatgctc ctgatcggca cactccgggt
    38581 tgtcacgcag gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtagtgc
    38641 ggctttcgct gaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg
    38701 cctgcccaac ataatggacg caatccgcaa gcccatgctg tgccagtacg gactcgaccg
    38761 cttcacggtc agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacgccg
    38821 ccaggcgatc gtcacccaga gaggcgatat ccgcgtcaat gccacaatga cctgcaaagg
    38881 ccatttccgc cagcgttacc agcaggcctc catcagagcg gtcgtgatac gccagcagct
    38941 tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc tttcagttgg acaacatcgc
    39001 gtacatctgc cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca
    39061 gcgcgttatt gcctttgccc aaatcaatca gcagcagtgc gttatcttcg gtacaaagct
    39121 gcggcgtgat ggtgtgacgt acatcttcca cgcgggcaaa tgcagaaatc accagcgaca
    39181 gcggcgacgt catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag
    39241 agtctttacc caccgggatc gtcaggccca gcgccggaca aagttcttca cccacggctt
    39301 taacggcttc atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg
    39361 cggaaagttt aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg gttaacgctt
    39421 caccgaccgc cagacgggca gaggcggcga aatccagcag cgcaaccggc gcacgctcgc
    39481 caatcgccat cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag
    39541 cgaccggcac ctgccacggc cccaccatct gatcgcgcgc caccatgccg gttacgctgc
    39601 ggtcaccgat ggtcaccagg aaagttttct ccgccacggt cggcagatgc agtacgcgtt
    39661 tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc atcgcccttc gctttcagcg
    39721 tttgtacatc gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga
    39781 tcggctgatt atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac
    39841 caatcaccgc gtagggtgcg cgctcacgct tacacagttc gtcaaacagc ggtaattgat
    39901 cggcagcaac cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc
    39961 tcatgcccgg ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg cgtccgccgt
    40021 cgctcaccag ctccggcatc gcgttagaaa gaccgcccgc accgacgtcg tggataaaca
    40081 gaattgggtt agcatcacca agctgccagc aacggtcgat cacttcctgg caacgacgct
    40141 ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgaccag
    40201 acgccatgga agacgccgcg ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa
    40261 cgaccagctt cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc
    40321 cgatcccgcc cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccattgtggc
    40381 tgttcacttt ttcttcataa gtacggaagt agccgttcag tgccggacga ccaaattcgt
    40441 tgttaaacgc cgcaccgccc agcgggcctt cggtcatgat gtccagcgcg gtaacaatgc
    40501 gctcaggctt accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg
    40561 atacggagaa accaaccaaa ccagctttcg gcttcgcgcc gcgcccggtg gcaccttcat
    40621 cgcggatttc accgccggaa ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt
    40681 ggttgtgagt ttcgactttc atcaggatgt gcgccggttc ctgatggaaa ttgtagcggc
    40741 ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat
    40801 ctttataagc agagagaacg tgatctggcg tggtttcgaa agtatttttg atcatcctga
    40861 acagcgattt cggctgttgt tcaccgtcga taatccagtc ggcgttaaaa attttgtggc
    40921 ggcagtgctc agagttcgcc tgagcgaaca tatacagctc gatgtcgttc gggttacgac
    40981 caagctttgt gaaagcatcc tgcagatagt caatttcatc ttccgccaga gccaagccaa
    41041 gacgcaggtt agcgtcgatc agcgcctgac ggccctgccc cagcaaatca acgcttgtga
    41101 ccggagtcgg ttgatggtgg gcaaacaact gctctgcatc atctaaatca aaaaagaccg
    41161 tttccatcat gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg
    41221 taccggcttc tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc
    41281 agttatgggc aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg
    41341 tcaccagcag gagtttgcct tgcggggcgt ggctggcgag tgccggacca tatttcagca
    41401 ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga
    41461 catactcggc gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt
    41521 tgttgattcg gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct
    41581 cgtcttctaa gctttcggtg tacccaaggg gggggaaacg ggcgtcatta taaagaatct
    41641 gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt
    41701 gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt
    41761 tatgagtaaa cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag
    41821 aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct
    41881 gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc
    41941 cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa
    42001 cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta
    42061 cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct
    42121 tgataatggt aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa
    42181 aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg
    42241 tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg
    42301 tcatgtggta gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg
    42361 gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact
    42421 cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct
    42481 cgccgtagcg ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg
    42541 cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac
    42601 gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac
    42661 gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa
    42721 atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg
    42781 ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac
    42841 cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt
    42901 gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga acgaacggat
    42961 ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct
    43021 gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt
    43081 acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc
    43141 ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga
    43201 gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc
    43261 gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc
    43321 aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca
    43381 aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg
    43441 aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac
    43501 tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt
    43561 cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca
    43621 cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat
    43681 caccagacca ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc
    43741 aatcgggcgg ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac
    43801 cggcacttcc cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccaata
    43861 ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat
    43921 tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac
    43981 tcgccaacga tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc
    44041 actgtacagc cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc
    44101 cagacaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag
    44161 attaacccgc ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga
    44221 gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg
    44281 aaccagcgga aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt
    44341 ctgtaaacgt gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca
    44401 gcgtgccgca cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac
    44461 aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc
    44521 ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa
    44581 accatactcc tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga
    44641 tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc
    44701 atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg
    44761 tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta
    44821 ccaagggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac
    44881 ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt
    44941 cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag
    45001 tgaagcgctg gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg
    45061 tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaataccg ccgctatgta
    45121 cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc
    45181 tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc
    45241 ctggaagctg atgaagattg gcttcaatgc tgccgcactg cgcgatatgc atgcgctact
    45301 cgcaacagta caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt
    45361 acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc
    45421 gattaccggc tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat
    45481 tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt
    45541 aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat
    45601 tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc
    45661 cgcccggttt gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca
    45721 tcaattgtga tatgtgtgaa atcaaataaa aacacattta ttttcatagt gatacagcaa
    45781 tgtgttattg aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa
    45841 cacaaagaac accactttct ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg
    45901 caaaaggcct gtgacataca acattaactt ttcagtcaac cgtagcacgt tcttgtatac
    45961 gccgtgtctg cggttttata taaccctggc tggaaacctc ttatacaaag tcgattcgcc
    46021 aacatcatag atgatcgcca ccttctggcg agaaactcct gatgcaatta gtcgcccggc
    46081 ctgcgcccat tattctggtg aaagtttggg acggcgaccg ccaattcgtc cctgtgcgcg
    46141 agcagcttcc agtcctgctt ttgttcgttc aacaatcagt tcacgttcca tttcagccag
    46201 ggcacccatc acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa ttgaatcagt
    46261 cagactacga aagttgatgc ctcgttcgcg caacgcctcc accagcacga caagatgccg
    46321 catactgcgc cccagccgat ccagcttcca gacaaccagt gtgtcgcctg ccgataatgt
    46381 cttgagcagt tttttcagcc ccggtctttc ggactttgta ccacttatct tgtcttcaaa
    46441 aatcagctcg catcctgcac agtt
//
LOCUS       DAZPEU010000013        44703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPEU010000013 DAZPEU010000000
VERSION     DAZPEU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.013"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(27..914)
                     /gene="rpnA"
                     /locus_tag="ACHU2L_000622"
     CDS             complement(27..914)
                     /gene="rpnA"
                     /locus_tag="ACHU2L_000622"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR1355611.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
     gene            complement(1117..1353)
                     /gene="feoC"
                     /locus_tag="ACHU2L_000623"
     CDS             complement(1117..1353)
                     /gene="feoC"
                     /locus_tag="ACHU2L_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR1355612.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1353..3674)
                     /gene="feoB"
                     /locus_tag="ACHU2L_000624"
     CDS             complement(1353..3674)
                     /gene="feoB"
                     /locus_tag="ACHU2L_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR1355613.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3691..3918)
                     /gene="feoA"
                     /locus_tag="ACHU2L_000625"
     CDS             complement(3691..3918)
                     /gene="feoA"
                     /locus_tag="ACHU2L_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR1355614.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4356..6677)
                     /locus_tag="ACHU2L_000626"
     CDS             complement(4356..6677)
                     /locus_tag="ACHU2L_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR1355615.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6774..7250)
                     /gene="greB"
                     /locus_tag="ACHU2L_000627"
     CDS             complement(6774..7250)
                     /gene="greB"
                     /locus_tag="ACHU2L_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR1355616.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7478..8197
                     /gene="ompR"
                     /locus_tag="ACHU2L_000628"
     CDS             7478..8197
                     /gene="ompR"
                     /locus_tag="ACHU2L_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR1355617.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8194..9546
                     /gene="envZ"
                     /locus_tag="ACHU2L_000629"
     CDS             8194..9546
                     /gene="envZ"
                     /locus_tag="ACHU2L_000629"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR1355618.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9622..11244)
                     /gene="pckA"
                     /locus_tag="ACHU2L_000630"
     CDS             complement(9622..11244)
                     /gene="pckA"
                     /locus_tag="ACHU2L_000630"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR1355619.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11623..13381
                     /locus_tag="ACHU2L_000631"
                     /pseudo
     CDS             11623..13381
                     /locus_tag="ACHU2L_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13525..14394)
                     /gene="hslO"
                     /locus_tag="ACHU2L_000632"
     CDS             complement(13525..14394)
                     /gene="hslO"
                     /locus_tag="ACHU2L_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR1355620.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14419..14820)
                     /gene="hslR"
                     /locus_tag="ACHU2L_000633"
     CDS             complement(14419..14820)
                     /gene="hslR"
                     /locus_tag="ACHU2L_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR1355621.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14831..15499)
                     /gene="yrfG"
                     /locus_tag="ACHU2L_000634"
     CDS             complement(14831..15499)
                     /gene="yrfG"
                     /locus_tag="ACHU2L_000634"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR1355622.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15564..17699)
                     /gene="igaA"
                     /locus_tag="ACHU2L_000635"
     CDS             complement(15564..17699)
                     /gene="igaA"
                     /locus_tag="ACHU2L_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR1355623.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            18019..18579
                     /gene="nudE"
                     /locus_tag="ACHU2L_000636"
     CDS             18019..18579
                     /gene="nudE"
                     /locus_tag="ACHU2L_000636"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR1355624.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18744..21296)
                     /gene="mrcA"
                     /locus_tag="ACHU2L_000637"
     CDS             complement(18744..21296)
                     /gene="mrcA"
                     /locus_tag="ACHU2L_000637"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR1355625.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21416..22195
                     /gene="hofM"
                     /locus_tag="ACHU2L_000638"
     CDS             21416..22195
                     /gene="hofM"
                     /locus_tag="ACHU2L_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR1355626.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22195..22734
                     /gene="hofN"
                     /locus_tag="ACHU2L_000639"
     CDS             22195..22734
                     /gene="hofN"
                     /locus_tag="ACHU2L_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR1355627.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22718..23158
                     /gene="hofO"
                     /locus_tag="ACHU2L_000640"
     CDS             22718..23158
                     /gene="hofO"
                     /locus_tag="ACHU2L_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR1355628.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23148..23554
                     /gene="hofP"
                     /locus_tag="ACHU2L_000641"
                     /pseudo
     CDS             23148..23554
                     /gene="hofP"
                     /locus_tag="ACHU2L_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23466..24704
                     /gene="hofQ"
                     /locus_tag="ACHU2L_000642"
     CDS             23466..24704
                     /gene="hofQ"
                     /locus_tag="ACHU2L_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR1355629.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVMPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25105..25626
                     /gene="aroK"
                     /locus_tag="ACHU2L_000643"
     CDS             25105..25626
                     /gene="aroK"
                     /locus_tag="ACHU2L_000643"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR1355630.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25683..26771
                     /gene="aroB"
                     /locus_tag="ACHU2L_000644"
     CDS             25683..26771
                     /gene="aroB"
                     /locus_tag="ACHU2L_000644"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR1355631.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26863..28155
                     /gene="damX"
                     /locus_tag="ACHU2L_000645"
     CDS             26863..28155
                     /gene="damX"
                     /locus_tag="ACHU2L_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR1355632.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28262..29098
                     /gene="dam"
                     /locus_tag="ACHU2L_000646"
     CDS             28262..29098
                     /gene="dam"
                     /locus_tag="ACHU2L_000646"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1355633.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29116..29793
                     /gene="rpe"
                     /locus_tag="ACHU2L_000647"
     CDS             29116..29793
                     /gene="rpe"
                     /locus_tag="ACHU2L_000647"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR1355634.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29786..30544
                     /gene="gph"
                     /locus_tag="ACHU2L_000648"
     CDS             29786..30544
                     /gene="gph"
                     /locus_tag="ACHU2L_000648"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR1355635.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30537..31541
                     /gene="trpS"
                     /locus_tag="ACHU2L_000649"
     CDS             30537..31541
                     /gene="trpS"
                     /locus_tag="ACHU2L_000649"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR1355636.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31811..32716
                     /gene="yhfZ"
                     /locus_tag="ACHU2L_000650"
     CDS             31811..32716
                     /gene="yhfZ"
                     /locus_tag="ACHU2L_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR1355637.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32733..33095
                     /locus_tag="ACHU2L_000651"
     CDS             32733..33095
                     /locus_tag="ACHU2L_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR1355638.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33179..34342
                     /locus_tag="ACHU2L_000652"
     CDS             33179..34342
                     /locus_tag="ACHU2L_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR1355639.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34343..35569
                     /locus_tag="ACHU2L_000653"
     CDS             34343..35569
                     /locus_tag="ACHU2L_000653"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1355640.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35566..36444
                     /locus_tag="ACHU2L_000654"
     CDS             35566..36444
                     /locus_tag="ACHU2L_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR1355641.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36455..36808
                     /locus_tag="ACHU2L_000655"
     CDS             36455..36808
                     /locus_tag="ACHU2L_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR1355642.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36820..38124
                     /locus_tag="ACHU2L_000656"
     CDS             36820..38124
                     /locus_tag="ACHU2L_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR1355643.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38136..39221
                     /locus_tag="ACHU2L_000657"
     CDS             38136..39221
                     /locus_tag="ACHU2L_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR1355644.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(39381..40112)
                     /gene="frlR"
                     /locus_tag="ACHU2L_000658"
                     /gene_synonym="yhfR"
     CDS             complement(39381..40112)
                     /gene="frlR"
                     /locus_tag="ACHU2L_000658"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR1355645.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(40212..40997)
                     /gene="frlD"
                     /locus_tag="ACHU2L_000659"
     CDS             complement(40212..40997)
                     /gene="frlD"
                     /locus_tag="ACHU2L_000659"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR1355646.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40994..41824)
                     /gene="frlC"
                     /locus_tag="ACHU2L_000660"
     CDS             complement(40994..41824)
                     /gene="frlC"
                     /locus_tag="ACHU2L_000660"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR1355647.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(41874..42896)
                     /gene="frlB"
                     /locus_tag="ACHU2L_000661"
     CDS             complement(41874..42896)
                     /gene="frlB"
                     /locus_tag="ACHU2L_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR1355648.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42917..44254)
                     /gene="frlA"
                     /locus_tag="ACHU2L_000662"
                     /pseudo
     CDS             complement(42917..44254)
                     /gene="frlA"
                     /locus_tag="ACHU2L_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(44549..>44703)
                     /locus_tag="ACHU2L_000663"
     CDS             complement(44549..>44703)
                     /locus_tag="ACHU2L_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR1355649.1"
                     /translation="KVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGG
                     CGPTAQ"
BASE COUNT        10415 a        11344 c        12234 g        10710 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tactcgccgc agccaactct
       61 tcttctgaca gcccggtaaa tcgcatgatg tctgcaagag ggactccgga ttcaagcatt
      121 attttggcta tatgcagggc tttggattgt tccccctcct gccgcaatct ttccgcaata
      181 gtcattaaac tctccttgtg tttcggtgaa cgttcggcaa cgccgtcgat aaaatcgtta
      241 aaacgtacag cgtcgccagt ttgcagtatg taattaaaca gccctttgat ttgtctgtca
      301 ttagcgtatc cactacttaa taagcaggcc atttgctcta ccagccccat cagatcgcgt
      361 tgacgaatat gtttttgaat taactccagc agcgccatac gtcggtgctg catgatttca
      421 tcatcaggca tgacggtgac atcaatcagc ggaaatgcgg aggcataaag ctgccttgcc
      481 agtttgggat cggcgaaaca atccagccaa cacagcgaat agggataggg gctttcaata
      541 ccgtggtaaa acaacaatgg caccaccatc ggcaacgttt tgtatccagc atcaaggtga
      601 ttttgcattg cggcaatagc gtaacgcatc atgcgaaatg cgatcagttt gtttgaggtg
      661 ctttgatgtt caatcagaca atagatgtat cctggtcctt gttccgtttt caccgaccac
      721 agcacatcgg aatagctttc acgcagatcg tcatcaataa agctcctcga ctccagcttg
      781 agggttttca tatcacagag cgcgtgaatg ggtgccggta aatgaaacgc aagaaaatcg
      841 cgtgccgtct ccggttggcg taaaaagagt ttgaacagcg catcatgtgg ggtggaactc
      901 tgctttttac tcatggcgtt ccttcacctg ttgtttgaat gccgcgactc tacgcgccac
      961 tccacatctg tgcattagcg agttttcatc cctgcgagac gctttcagag gaacttacgt
     1021 tgcgctgcaa gaattgcatt tttatggaaa gccgcacgca aaaaagcccg gatgcaaacg
     1081 cataccgggc ttttcaaaac aggcgatgga gtatggttaa cgcagcgccc accactcgcg
     1141 cagacaggct ttgccttccg ggcagctttt acaactgcca gagaggcagc cgtcaggttc
     1201 ttcctgaatc cgcacggctt tgcccatgct ttccagctgt tgcagcatgg cgttaatcat
     1261 tggctgcgga gtgttcaatg tctggcttat ctgggtcgct tccatacggc cccgtaacgc
     1321 cagcaaatca cgtacctgaa taagtgaagc cattaatggc aatcaccggt ggtgctggct
     1381 gcgcagcaac tgcttaccga cttgcgggtt gccagcagtt cgatatccac ccggctacgc
     1441 gcgcggcgca gcagaccgat agctacgatg ttaaacagga ttaccgccag aatgcacacc
     1501 aggctgtaag ttgggtgctg actgtagctg gcgacctgat agaacaatgt tgccagtgag
     1561 taagcgatat tcagccccca caggatggag aagcccatcc agccacggct tgattcacgg
     1621 gcgatggctc ccatcaccga gatacatggt acatacagca ggacgaaaat caggtagctg
     1681 tatgcggcag ctgcgctacc gaatttctga tccatcacgc ccatcgcccc ggtacccatt
     1741 tcaccgttgc ctttgctagc ttcaatgggg ttcatcagta cgctaaggct gaaggtgtct
     1801 ttcaggctct gccaggtttc atctaccgca ctgaacagct cttcaccgag gttgaattcc
     1861 gccggattga actcttcgtc ctgaatattt tctgcggtgt agagggtgtt gagcgtaccc
     1921 accaccactt cttttgccat tgcgcccgta aacaggccaa ccgttgcctg ccagttatct
     1981 tcatgtacgc caatcggctt gaagacaggg gttatcaccc ggctgacgga agccagcgcc
     2041 gagtcgttga tgttatcgac gattttcccg ctcaacgaga agctgttgaa agcgctcagg
     2101 aaaatgctga cgatgataat cactttacca gcacgcagaa cgaagccctt cagacgctgc
     2161 caggtctgga taatcaggct tttaacgtgt ggtacatggt agaccggcag ctccatgaca
     2221 aacggcgtcg cttcaccgcg catgatggtg tacttgagca tcaggccagt cagcaccgcc
     2281 atcacgatac cgagcatata cagcgagaaa accgccaggg caccgttctg cccgaagaag
     2341 gcagccgcga atactgcgaa gatagccaga cgcgcgccgc aggacataaa cggtgccatc
     2401 atgatggtca tcagacgttc acgcggtgca tcaagcgtac gtgcgcccat taccgacggt
     2461 acgttacaac cgaaaccgac gatcagcggc acaaaggatt tccccggcaa gcccagcgcc
     2521 tgcatcaggc ggtccatcac aaacgctgca cgcgccatat agccggagtc ctcaaggaag
     2581 gagaggaaca ggtacatcat gccgatctgc ggcaccagtg gcagcacggt gttaataccg
     2641 ccacccaggc cctgggcgag gaagatagtc agccagtccg ggaagtggag cgtgtagcca
     2701 atccattgaa taccatgcac aaatagcgcc acggagccga cgttaaacag cggctgtaac
     2761 gccccgccga tgttgatagc cagcaggaac atcaggtaca tcacaaagag gaaaatcggc
     2821 agaccgagga aacggttgag cacgatttta tctaccgcag tggtgaaacg gctgggttct
     2881 gccgtcaggg tgttgcttac cacatcacag atggcagcaa tgcactggta acgcgcatcg
     2941 gcaatgtgca gcgccggatc atccatttca ttacgcagac gggcgagggt ggcatccaga
     3001 tgctgcgacg cttcaccggc gtaggcgcgg ctgtagatat cgccttccag catttgcagg
     3061 cccagccagc gacgttgttt cagcgggatg tcggaaggca tcacttttgc cagtgaatct
     3121 gcttcgttga gtagcggctg tgcgtaatgc accagttcca cattctcgtt agctttatag
     3181 cgatcaatcg ccagcttgag cgcttcaata ccgcgaccac gggttgaaac cagcgggatc
     3241 accgggcagc caagacgtgc cgatagagca tcaatttcaa tacgaatatt ttgcttctcg
     3301 gcaatgtcga gcatgttcag cgccacgatg cagggaatgc cgagttccag cagttgcagc
     3361 gtcaggtaca ggttacgctc aaggttagac gcatccacca cgttaatcag caggtcggcg
     3421 tcgccactca aaatgtagtg acaggcgatt tgctcatcga gcgaggtctg cgatgagatg
     3481 gtggtcagag aataggtgcc gggcaggtcc accagcgtga cctgatgatc ggtggtggag
     3541 aattgccctt ctttacgttc gacggtaacg ccagcccagt tacctacacg ctgacgtgcg
     3601 ccagtgagct ggttaaataa ggttgtcttg ccagaatttg gattaccaat taagccaatg
     3661 gttaattttt tcattgttgt tatcaccgta ttaacaggaa accgcttcca cttctaataa
     3721 ggccagatct tttttgcgta ataccaggct cacacgacgg gtttcgatat gaatggggtc
     3781 gccgagtgga gcgacgcgca ccacatcaaa agaggagcca ggtaacatgc ctagagaaag
     3841 cagtttttgg cgatatgccg ggctgatttc acgggaaaag ccagtgattt tccacgcagt
     3901 atctggagtg tattgcatag gtgcctactt gtttctcatt aactggataa ctacctcatg
     3961 gggcgtttga ccgcgacatg agaccagatg atttgccaac aaaggagaga ttaaataatc
     4021 aacaacacaa tgataatgag aatggtttct atcagcaata cttaaaatgt gtgcgaatgt
     4081 tggtttgaat aataatctga ctcaatgttg atgtggctca aggttatatc gcagcgaaaa
     4141 tataattcga atagtgagtt acccggactg gctaatgttt aattaaggtt tcagttgtta
     4201 atgaagtgat aaatattaac ggagtatatt gtgagtttgg ctgtggaata aaagtgccgg
     4261 agatattctc cggcactttt atgtcaggca aagcgttcgt tgccagatgc ggcgtgaacg
     4321 ccttatcgcg caggtttagg ggccagtccg ggcgtttaac gttttttacc catcgctgcc
     4381 gccagcgcat ccatcatcgc gctattaccg gcaggctgcg cttcacgacc gcgtggtttg
     4441 gctgccgggc ggttgttttg cgggcgttca ttaccgccgc cgcgacgaga gttggtttcg
     4501 ccaggctgct cgtccagacg catagtcagg gcgatacgtt tacgctgaag atccacttcc
     4561 agcactttca ccttcacaat gtcgcccgct ttcaccacgg tatgcggatc ttccacaaac
     4621 ttgttcgaca atgaagagat gtgaaccagg ccgtcctgat gcacgccaat atcgacaaac
     4681 gcgccaaagt tggtgacgtt ggtcactgcg ccttcgagga tcatacccgg ttgcaggtcg
     4741 ttcattgtct cgacgccatc ggcaaactga gcggttttaa attccggacg cggatcgcga
     4801 cccggttttt ccagctcttt gatgatgtca gttactgtcg gcacaccgaa tttttcatca
     4861 gtaaagtcag acgctttcag gttacgcagt tcgctgctgt tacccatcag atctttcagt
     4921 gcctgctgtg ttgctgccag aatgcgttcc accaccggat aggcttccgg atgaacggta
     4981 gaggcgtcca gcgggttatc accgtggtta atgcgcagga agcccgcgca ctgctcgaag
     5041 gctttcggcc ccagacggct gactttcagc agttgctgac ggttctggaa ctggccgttc
     5101 tcatcgcgcc aggccacgat gttttgcgcc atcatgcgcg tcaggcccgc cacgcgggtt
     5161 aatagcggaa cagaagcggt gttgagatcg acgccaacgg cgtttacgca gtcttctact
     5221 actgcatcca gtttgcgggc cagttgcgtc tggctgacgt catgctgata ctgacctacg
     5281 ccgatagatt tcggatcgat tttcaccagc tccgccagcg gatcctgcaa acggcgggcg
     5341 atagataccg caccacgcag cgaaacgtcg agatccggga actcctgcgc tgccagctcg
     5401 gaagctgagt aaaccgacgc gccagcttcg ctgacgatca ctttctgtgc ggtcactttc
     5461 gggaactgct tctgcacgtc gagatagaaa cgttcagttt cgcgggaagc tgtaccgttg
     5521 ccgatcgcca ccagttcaac gttatgtttt tcacacaagg cagcaacggt catcgctgct
     5581 tttgcggctt gtccggtgtg cgggtaaatg gtatcggtcg ccaccagttt gccagtggca
     5641 tcgaccaccg ccacttttac cccggtacgc agacccggat cgaggcccat cgttgcacgc
     5701 agtccggcag gggccgccat cagcagatcg tgcaggttac gggcaaagac gttgattgct
     5761 tcatcttccg cgcgttcgcg cacggtaccc atcagttcgg tttccagatg catcagcacc
     5821 ttgatgcgcc aggtccagct cactacgctt ttgcgccagc tatccgccgg ggcattgttc
     5881 aggcgcaggc caaggtgatc catgatgatt tgctcgcaat agctctcttt gggcggctca
     5941 tcgaactgcg gatcggcatt cagcgaaagc tggagtacgc cttcgttacg cccacggaac
     6001 atcgccagcg cgcggtgaga aggcaccgtg gacaacggtt cgtgatgatc gaaatagtcg
     6061 cggaatttcg ccccttcctc ttctttaccg ctcaccaccg tagaaaccaa atgcgcgttc
     6121 ttccacagat aatcacgcac tttcgccagc agcgtggcat cttcggcaaa ccgttccatc
     6181 aggatatagc gcgcgccatc cagcgcggct ttggtatctg ccacgccttt atcggcatca
     6241 acatattgtg cagcggcgac ttctggcgtg tgtgacggat cgctccacag caggtcagcc
     6301 aacggctcaa gccctgcttc aatggcgatt tgcccgcggg tgcggcgttt aggtttgtag
     6361 ggcaggtaga ggtcttcgag ttcggttttg cttagggtgg cgttgatggc cttcgccaga
     6421 tcatcggtga gtttgccttg ctcggaaatg gatttgagga tcgcctgacg tctctcttcc
     6481 agctcgcgca gatagctcag acgcgtttcc agattacgca gctgcgtgtc atccagaccg
     6541 ccggtgattt ccttacgata acgtgcgata aacggcacgg tattcccttc gtcaagcagg
     6601 cgaacggcag cgtcaacctg ttccgggcgc gcctgaattt cacccgcaat aatgcggcag
     6661 aacgaatcat tcatcatggt ttgagttcat cttttcggat caaaaatcag gggatagtta
     6721 tacggactgg ctggcaaaaa tgccagccat cggcaggagg ttaagactct tccttacggt
     6781 ttcacgtact cgatagcatt aacataccag ttcgcttccc cggcaggggt attcaccacc
     6841 gccagatcgc cgacttcttt tttcagcaat gcgcgggcca tcggggaatc gatagagatg
     6901 taatctttac ggccaaaaat ttcatcgtag ccgacaatac ggaaacggtg agtcacgcca
     6961 tcgtcgtttt caatctccac ccacgcgcca aaaaagactt tgccttcctg ctgaggggag
     7021 taatcgacga ttttgagatt ttccaggcat ttagtgagat agcgcacgcg acggtcgatt
     7081 tcacgcagac gctttttatt atactgatag tcagcatttt cgctgcggtc gcccagactt
     7141 gcggcccagg tcaccttttt tgtgacctcc gggcgttctt cacgccagag ataattaagc
     7201 tcttgtttga gtttttcata cccttcccgg gtaaccaggg gcgttttcat ctcgttgatt
     7261 ccctttgtct gtttgataat gcgcacattg ggtataacgt gatcatatca acagaatcaa
     7321 taatgtttcg ccgaataaat tgtatactta agctgctgtt taatatgctt tgtaacaatt
     7381 taggctgaaa ttcataccag atttagctgg tgacgaacgt gagctttttt aagaatacac
     7441 gcttacaaat tgttgcgaac ctttgggagt acaaacaatg caagagaact acaagattct
     7501 ggtggtcgat gacgacatgc gcctgcgtgc gctgctggaa cgttatctca ccgaacaagg
     7561 cttccaggtt cgaagcgtcg ctaatgcaga acagatggat cgcctgctga ctcgtgaatc
     7621 tttccatctt atggtactgg atttaatgtt acctggtgaa gatggcttgt cgatttgccg
     7681 acgtcttcgt agtcagagca acccgatgcc gatcattatg gtgacggcga aaggggaaga
     7741 agtggaccgt atcgtaggcc tggagattgg cgctgacgac tacattccaa aaccgtttaa
     7801 cccgcgtgaa ctgctggccc gtattcgtgc ggtgctgcgt cgtcaggcga acgaactgcc
     7861 aggcgcaccg tcacaggaag aggcggtaat tgctttcggt aagttcaaac ttaacctcgg
     7921 tacgcgcgaa atgttccgcg aagatgagcc gatgccgctc accagcggtg agtttgcggt
     7981 actgaaggca ctggtcagcc atccgcgtga gccgctctcc cgcgataagc tgatgaacct
     8041 tgcccgtggt cgtgaatatt ccgcaatgga acgctccatc gacgtgcaga tttcgcgtct
     8101 gcgccgcatg gtagaagaag atccagcgca tccgcgttac attcagaccg tttggggcct
     8161 gggctacgtc tttgtaccgg acggctctaa agcatgaggc gattgcgctt ctcgccacga
     8221 agttcatttg cccgtacgtt attgctcatc gtcaccttgc tgttcgccag cctggtgacg
     8281 acttatctgg tggtgctgaa cttcgcgatt ttgccgagcc tccagcagtt taataaagtc
     8341 ctcgcgtacg aagtgcgtat gttgatgacc gacaaactgc aactggagga cggcacgcag
     8401 ttggttgtgc ctcccgcttt ccgtcgggag atctaccgtg agctggggat ctctctctac
     8461 tccaacgagg ctgccgaaga ggcaggtctg cgttgggcgc aacactatga attcttaagc
     8521 catcagatgg cacagcaact gggcggcccg acggaagtgc gcgttgaggt caacaaaagt
     8581 tcgcctgtcg tctggctgaa aacctggctg tcgcccaata tctgggtacg cgtgccgctg
     8641 accgaaattc atcagggcga tttctctccg ctgttccgct atacgctggc gattatgcta
     8701 ttggcgatag gcggggcgtg gctgtttatt cgtatccaga accgaccgtt ggtcgatctc
     8761 gaacacgcag ccttgcaggt tggtaaaggg attattccgc cgccgctgcg tgaatatggc
     8821 gcttcggagg tgcgttccgt tacccgtgcc tttaaccata tggcggctgg tgttaagcaa
     8881 ctggcggatg accgcacgct gctgatggcg ggggtaagtc acgacttgcg cacgccgctg
     8941 acgcgtattc gcctggcgac tgagatgatg agcgagcagg atggctatct ggcagaatcg
     9001 atcaataaag atatcgaaga gtgcaacgcc atcattgagc agtttatcga ctacctgcgc
     9061 accgggcagg agatgccgat ggaaatggcg gatcttaatg cagtgctcgg tgaggtgatt
     9121 gctgccgaaa gtggctatga gcgggaaatt gaaaccgcgc tttaccccgg cagcattgaa
     9181 gtgaaaatgc acccgctgtc gatcaaacgc gcggtggcga atatggtggt caacgccgcc
     9241 cgttatggca atggctggat caaagtcagc agcggaacgg agccgaatcg cgcctggttc
     9301 caggtggaag atgacggtcc gggaattgcg ccggaacaac gtaagcacct gttccagccg
     9361 tttgtccgcg gcgacagtgc gcgcaccatt agcggcacgg gattagggct ggcaattgtg
     9421 cagcgtatcg tggataacca taacgggatg ctggagcttg gcaccagcga gcggggcggg
     9481 ctttccattc gcgcctggct gccagtgccg gtaacgcggg cgcagggcac gacaaaagaa
     9541 gggtaaataa acgggaggcg aaggtgcctc ccgttttgct ttctataaga tactggatag
     9601 atattctcca gcttcaaatc attacagttt cggaccagcc gctaccagcg cggcacccgc
     9661 aggggtgtcg gtgtatttat cgaagttgtc gataaacagt ttcgccaggg tttcggcttt
     9721 ttcctgccac tgttccggag aagcgtaggt gttacgcgga tcgagaatct tcgtgtctac
     9781 gcccggcagt tcggttggga tcgccaggtt aaacatcggc agagtgaagg tttctgcatt
     9841 atccagcgaa ccgttgagga tggcgtcgat aatggcgcgg gtatctttaa tcgagatacg
     9901 tttgccagtg ccgttccagc cagtgttaac cagataagcc tgcgcgcccg ccgcctgcat
     9961 acgtttcacc agcacttctg cgtactgagt cgggtgcagc gacaggaacg ccgcgccgaa
    10021 gcaagcggag aaggttggcg tcggttcggt gatgccacgc tcagtaccgg ccagtttggc
    10081 ggtgaagcca gagaggaagt gatactgggt ttgatcggca gtcaggcgag aaaccggcgg
    10141 caacacgccg aaagcatcag cagtcaggaa gataacctta gtcgcgtggc ccgctttgga
    10201 aaccggctta acgatgttat cgatgtgata gattggataa gaaacgcggg tgttctcggt
    10261 ttttgaacca tcatcaaagt cgatagtgcc atcttcacgc acggtgacgt tttccagcag
    10321 cgcatcacga cggatagcgt tgtagatttc aggttccgct tctttcgaca gcttgatagt
    10381 ttttgcgtag cagccgcctt cgaaattaaa cacgccgtca tcgtcccagc cgtgttcgtc
    10441 atcgccaatc aggcggcgtt tcgggtcggt ggaaagggtg gttttaccgg tgccggaaag
    10501 gccgaagaac accgcaacat cgcctttctc gccaacgttg gcggagcagt gcatagaagc
    10561 gatacctttc agcggcagca ggtagttcat catcgagaac atccctttct tcatttcgcc
    10621 gccgtaccag gtgccgccaa tcagctgcat gcgctcggtc aggttaaacg ccacgaagtt
    10681 ttcggagttc agaccctgtt ctttccactg cgggttagtg cacttcgcgc cgttcataac
    10741 gataaagtct ggtttgaaac ctgccagctc ttcatcgctc gggcgaataa acatgttttt
    10801 gacaaaatgc gcctgccagg ccacttcggt gatgaaacgg acggaaagac gagtatccgg
    10861 gttcgcacca cagaaagcgt cgacaacgaa cagacgtttg ccggaaagct gcctggtcac
    10921 caggcctttc agatgctgcc aggtttccgg agagagaggt ttgttgtcgt tcttaccttt
    10981 gcctttgtct gcccaccaga aagtatcgcg agtggtatcg tcacggacga tatacttatc
    11041 ttttggtgaa cgaccggtga agatcccggt atcgacggca acggcaccca gattagttaa
    11101 caccccgcgc tcataacctg tcaggctcgg atcgagctct tcctgataca gcaggtcgta
    11161 gcttgggttg taaacgatat catgtacgtc actgatacca taagcctcga gttcttgcgg
    11221 gggcaaacca ttgttaacgc gcatttcact gctccttagc caatatgtat tgcctgaata
    11281 gtaaagtctt tttgggggtg ttaaccgcga caaggctcat agatttacgt atctggagaa
    11341 attcatattt gacaaaaaac tccgtgattc ctgtcacgaa acggttgcta ttatcgcaga
    11401 aatagcattt taggtgtgga aaatgttctc aaaaggttaa atcttggttt atatggcggg
    11461 aatattgtaa gtggaatcgc attcctgaat gaaattgatg caaattcaac cggtaaagta
    11521 tcgattcacc acgctaactt tttttgatta ttttctgaaa aaagcaaaat actactgccc
    11581 ggatgcgata attaacccac cttttgattc tggataacca acatggataa cgttgaactt
    11641 tcaccggcaa cacgctgggg gatgattgct accggattac ttcaggggct ggtttgctac
    11701 ctgcttatcg cctggcttgc cggaaaaaat cacagttgga ttgtttatgg cgtgcctgcg
    11761 accgtcgcgt tttcatccgt tttactcttc tccgtgatct cttttaaaca gaagcgcctt
    11821 tggggatggc tggcgctggt gtttattgcc acgacaggga tggctggcgc tggtgtttat
    11881 tgccacgaca gggatgagcg gttggctgaa gtggcaaact gatggcatga acccctggag
    11941 agctgaaaag gcgatttggg attttggctg ctatctgctg ttgatggcaa tgttgttgct
    12001 accgtggata caacaaagcc ttcgtatccg caatggcagt agccgctata gctactttta
    12061 ccagtcagta tggcataacg tacttatatt actggtgatt tttctctcca atggcctgac
    12121 gtggctggtg cttttactgt ggggtgagtt gtttaaactt gttggcatca cgtttttcaa
    12181 tacgctcttt tttgcaaccg actggtttat gtatcttaca tttggtctgg ttaccgcact
    12241 ggcggtgatc ctcgcgcgta cacagtcacg tttaatcgac tctattcaaa agttgttcac
    12301 gctaatcgcc acggggttgc tgccgttagt atcattgcta accctgatgt ttatcatcac
    12361 cctgccgttt acgggcctga gcgcgatttc tcgccacatc tccgccgccg ggttgctgtt
    12421 aacgctggcc tttttgcaat tgatcttaat ggctattgtc cgcgatccgc aaaaagcgtc
    12481 acttccctgg acggggccgt tgcgttgcct gattaaaacc gctttgctgg ttgctccgct
    12541 gtatgtgttc gtcgccacct gggggttatg gctgcgggtc gctcagtacg gctggactgt
    12601 cgaccgcttg cagggcgcgc tggcggagct ggtgttactg gtgtggtcgc tggggtattt
    12661 tgtcagtatc gtctggcgta atgggcaaaa tccccttgtt cttcagggca aagtgaacct
    12721 tgcggtttcg ttattggtgt tggtgatact ggtgcttctt aattcgccgg tgctggacag
    12781 tatgcgtatt agcgtgaaca gtcatatggc gcgttatcag agcggcaaaa acacgccaga
    12841 ccaggtaact atctacatgc tcgaacagag cggtcgctat ggacgtgcgg cgcttgagtc
    12901 gctgaaaagc gatgccgggt ttatgaaaga cccgaaacgc gcgcgggatc tgctgatggc
    12961 gttagatgga gagcaacatc ttcaggaaca ggtatcggaa aaagtattcg ccgaaaatgt
    13021 gttaattgcc cctggttctg ttaaaccgga tgcgacattc tggtcggcct taatccagga
    13081 tcgctataac gtgatgacct gtattgaaaa agacgcctgc gtactggtcg agcaagatct
    13141 gaatagtgat ggtcaggcgg agcggatcct gtttgctttt aatgatgaca gagtcattgt
    13201 ctatggcttt gactcagaca gaaaagaatg ggacgcgctt gatatgagtt tacttccgaa
    13261 cgaaataacg aaagaaaaat tactcacagc tgcgaaggat ggaaaactgg ggacgaggcc
    13321 gaaagcgtgg cgcgatctga cggtagatgg tgaaacgctg gaaataaact taagtaagtg
    13381 acaacgcttt tatcggatac gacgctgtat ctgaccaaaa taatttgcga tttatttcat
    13441 aggcgctggt aagcgcagca catcaggcca ttaagtcgta aaatccggca gagccctctc
    13501 tgccggacat actcattaat gaacttacgg atctgccgga gacgcgttgt tgcggatttc
    13561 agcaatatcc atcgcattga acagatagtg gttaccgcag taatcacaat gcatgtcaat
    13621 ttcgccatct tccgccagga tgctatcaac ttcttcatca ggcagcgttt tcagcgcatc
    13681 ggcgcaacgt tcacgcgagc aggtgcattt gaactccaca tcctgcggat cgtaaaccgt
    13741 cacctcttct tcgtgataca aacgccacaa cacttcgttt gccggtaagg tcagcagttc
    13801 ttcggttttg atggtttcgg ttagcgtcgc caggtggtca aagtcgtcct gttgggtatt
    13861 ttgcgcaggc attacctgca acaacatacc gcctgcagcc ggtttgccgt ctacgtcgcc
    13921 ggtgcgaata aacaggcgcg tcggcagctg ttcagaacgc ataaagtaat cttctaggca
    13981 ggccgccagg gtatcacctt ccagaccaac tacgccctga tagcgttcgc cttcgctcgg
    14041 ggtaatggtg atcaccacgt aaccattgcc gaccagcgtt ttcaggtcgg cattttctgg
    14101 aatttcgccc tgcacgcgcg ccacaccgcg catttgctgg ttattgttac cgttaataac
    14161 cgccagattc atcggaccgt cgccctgcag ctgtacggtg atatcaccat caaacttcag
    14221 cgtagcggtt aacaggctgg tcgcaaccag cagttctgcc agcacgtttt taacgggctg
    14281 cggataatcg tggttctcaa ggatctgttg cagggtttcc gaaacggtta ccagttcgcc
    14341 gcgaacggca aaattttcaa acagatagcg atgtaattgg tcatgttgcg gcataatcat
    14401 ctctcttgca ggtgacagtt attcactgtc gccgtgttta aatcgtaaca ggtcgcggcg
    14461 ctcttttttg tccgggcgtc ggtccgggtg cggcatggtt aaggcattaa gtttacgtgc
    14521 catcgccatt ttttcgcgtt tctctacact ttccgcagtc tcttcataca gcaaggctgc
    14581 ctcgctggcg gggcgacgct gttcagtaat cgcctttaca atcaccgtgc gttcgtcatt
    14641 tccctggcgc agagtgagcg tggcattcag ctcgacgatt ttgctcggct tgctgcgctg
    14701 cccgttgtaa tgcaccttac cgccttcaat catttcacgg gccagcgcgc gggttttata
    14761 aaaacgggca gcccatagcc atttatccag tcgaacctca acagcaggtt tctctttcat
    14821 ggcgtctcct tcacattagc gaggggatca ggcggcggta gtcattcagt gacggatggc
    14881 gttgatactg tttctcggca atcccggaat caggattagt cacgccgagg cagtaacgaa
    14941 taccaaattg cgcggcagca tcgagaatcg cttcgctgtc atcaataaac agcgttcttt
    15001 cagctttcag acccgtagct tcggccaccg catgccataa ccgctgatcc tctttcggat
    15061 aaccaaatgt gtgggtggaa agtaataaat caaggtgtgc gtccagaccc gtatgctcaa
    15121 gttttaccgc caggttgtgc ggatgagcat tggtgagcaa aattcgctgc ttaccgctgg
    15181 ctttcagtgc ctcaagaaac ggaatggtat cttcacgcag tacggcgcgc ggtcccatct
    15241 cggtggtcat cgcacagata tccagaccca gttgctcact ccagtaatca agacagtacc
    15301 agtttagcgt atgctgtacg tcgtgatatt gctggcgcat atattccatc gcttcctgtg
    15361 gcgtaacccc gtttttcgcg ccccatgttt caggcaccag cttttgccag aaatagttat
    15421 cgaaggcgag gtcgagcaac gtgccgtcca tatccagcag aacggtatct acgtcctgcc
    15481 aggcaatgtt gatatgcatg agggaaatct ccagagtgaa gcaatttgcg cgacagggta
    15541 gcataacctg ccgcgcaaac gtgttattcg ataaggcttt ctgaaggggt gatcagttgc
    15601 gggttcaggc agctttcata ataggcctga atctccatca tgcgagtgcg atgacgctgg
    15661 taacgacgcc aggcctgtac gccattgtaa atggcgctac ccagcatcgt cagtagcagc
    15721 aatgtggtgc tgagatagcg ccacaggccg gaacgatccg ggatcggatg aaggccaata
    15781 tgctgcgtac cattggcgtc agtgaagatt tttgtgacga taccttcggc gttaaacggc
    15841 gtatgcatca gcatttgtgc cagtttctgg aaagcgttcc actgttcttg cggcgggtag
    15901 tcgtaaagtg atgccgaagg ccagggctga tcaacaaaat cgctgccttc gtcgctgaca
    15961 atcaggaatc cgccgggggc cggactgttt agtgattgtg ccgctcgcgc cgtttcatgc
    16021 gtgataaacg gcgcggtgga ggttgccacc aggttatcca gcgattcagc actcaccggg
    16081 cgtaataaca cattcacgcc atcgagcttc ccggcgttgg cgcgttttac cagcgcgtcc
    16141 cagtctttac tgttgccgag attgaccagc gcatttttca gtcgcacaca gtcatctttg
    16201 gcagaacata aatccgctgt cttcagtaca atgtcgccaa aatcatcaag caataccatg
    16261 ccggattttt gaatcgctga acgtaatgag gcactgacgc gagattcatc ttccggttta
    16321 gggtgcagct ggcgattaac tgcttcagtc aatgccgtcg ctttgttgac cagttcagat
    16381 tctggtaatg gtaatgagcg ggcgtcattc cagatgatct gcgagcagtc aaacggtaaa
    16441 aaaggtgaat tggttttcgc gctccaggtt ccggaagtgc gaatattaca cattcccgta
    16501 ccgctaatac gcaatgtatc gcctacccgc acgccagcgt cagccagttg ttttacgctg
    16561 gtggcttcaa tggtctgcgc gcctttcatc catgagagag tgaatttcag cggcatatcc
    16621 agcgggatcc agaataacag cataaacagc actagcagcg agcccgcagc gataatcgta
    16681 ctgcgcagcc agtgctgtaa cggaaagttt tttacttcat catgcagcga aagatatcgc
    16741 ccctgacgca ctacgtggcg gtcgagatag atatcaatat cggtttgttg accgagatcc
    16801 tgagcaatgt atggctgcca gtgtgcggga tagaccaggt cgataatacc gagtgaaata
    16861 ttgttgatct gttcctgatc gttttcgcca aacaatcccc aacgacgggg tgtaccgcgc
    16921 agacaatgaa tttctcgcag ggaggatttt gccgggggcg cgaatagccc ccacagacct
    16981 gcgcccagca gcagtaatgc gccgcctgcc agccacggaa caaatacatc cggggtaatc
    17041 aggcagaaaa agaacatcag gaaggaggcg acgatcagca acgcttcacg cagcccgcgc
    17101 ggacgactca gcgcatattc ttcatgcgtt tctttgcgaa tattgagtag ctcgatttgc
    17161 tcactctctt ccccgcgaat cgacgcctgc gtggacggaa caggttgcaa ggcatagccg
    17221 cgcgtttcct gcatgtactc ctgcagcgta tgaccgttga gtgaaataac caacggcagc
    17281 gaatcggtat gaatcagttc aacggtattc tcatcgttga tgtactgttc ccagaagggg
    17341 ggcaggtgga cttctaccga atcgaggtag taacgccatt tattcggatc gtcggtagag
    17401 atgccgtaac gtgtgatagc gtgtgtcagc atcatgacgt tgttgctttc ggcattcagc
    17461 gccagagaga tcggtgccgc gctagcttcc gttgggccag gcacctgtaa tacctgcgtc
    17521 aggctctcaa gataattttc aacggcgcta cgttcttcag gtgtgagttt acgcgtttgc
    17581 gcatccgcga aggcgttggt ccagggcagc tgacgccgtc tggatcgcac ttttatcagc
    17641 catcccgcaa gtagtgagca ggccagcaaa gcagctaaaa aaatcacaat ggtgctcatg
    17701 ctttccccat cttacttagt ctgtcaggcg tggtcagtcg caccggacac aatacatgat
    17761 tctgcggttg gctatcggca agcgtggagc ttaacttgag catcattaag cacatcacag
    17821 taaccgctga tcatagcaaa ccctctgaag gcggaatatc agcaaattcc tggagttatt
    17881 tcaacctatt gacttttaat ttgatttgag gacgattatt tattaagtta cataaatgta
    17941 aataacatgc aattcacatt gccgaaagcg tgtccgatat cgcacaataa ccgctgattt
    18001 cacagcatag atcccacgat gaacaaatca ttacaaaaac ccaccattct gaatgttgaa
    18061 actgtagccc gttcccgact gtttaccgtc gagagcgtgg atctggagtt cagcaatggc
    18121 gtgcggcgtg tttatgaacg aatgcgtcca accaaccggg aagcagtgat gattgtgccg
    18181 attgtggacg atcacctgat cctgatccgc gaatacgcag tgggaactga atcctacgaa
    18241 ttaggttttt cgaaaggatt aattgatccg ggtgaaagcg tctacgaagc cgctaaccgc
    18301 gagctaaaag aagaggttgg atttggtgcg aacgatctga cttttttgaa gaagctcagc
    18361 atggcaccgt cttacttttc cagcaaaatg aatatcgtgg tcgcgcaaga tctctacccg
    18421 gaatcgctgg aaggcgatga gccagagccg ctaccacagg tgcgctggcc gctggcgcat
    18481 atgatggatt tgctggaaga ccctgacttc aacgaagcgc gtaatgtcag cgcgctgttc
    18541 ctcgtgcgcg aatggttgaa agggcagggg cgagtgtaaa tgcgtgaagt ttgcctgatg
    18601 cacaacgctt atcaggctta tggggttcct gcaatatatt gatttttcac gattttgtta
    18661 gccggataac gcgttcaccc gcatccggca taaacaaatg cactttgtca gccaactgaa
    18721 aaggcgccga agcgcctttt taatcagaac aattcctgtg cctcaccatt atcgataatg
    18781 gtcgttccca cctcgtgcac cgcctgttgt gtcggctgcg taccttcgat gaaatactct
    18841 tcgcggctgt tgccaccatt agctaactgc ccggtgctgc gatcgatatt caccgtcaca
    18901 atacccggag gcggcgtcag cggctgctcc ggcacacctt caagaacggc tttcatataa
    18961 gcgtcccatg caggctgggc actcttggca ccgccttcgt aacctgagat ctgatcttta
    19021 atcgctccgg aagccgttgt atgaccgaga ttacgacggt gatcatcaaa gccaatccag
    19081 accgaggtca caacgcccgg accgtaaccc gagaaccacg catctttcga actgttagtg
    19141 gtaccggttt tcccgccgat atcgcgacgc tgcaaatcac gacctgcacg ccagccggta
    19201 ccctgccagc ctggttcacc aaagatattg gtgttcaaag cactcttaat caggaatgcc
    19261 agcggagtgt tgatgacgtg cggtgcgtac tcctgcgcgc cagtcttcgc cactaacgcc
    19321 tgatttgcct gctccagctg cggcattggt acagaaacat tctgctgctc gcgggagata
    19381 gcgacatctt caacatcgtt attttccagc acgttcgatt tctgcgtatc accgtaaatc
    19441 actggaatat cgcattccgg gcaggctact ttcggtttcg cttcgaaaat cacgccgccc
    19501 tgatcgtttt caattttgct gataaaccac gggtccacta ggaagccgcc gttcgccatg
    19561 accgcgtagc cgcgcgccac ctgcattggg gtgaaggacg ctgaacccag cgccagcgat
    19621 tcggtgtgga caatgttttg cgccgggaag ccgaagcgtt gcagatattc tgcagcgtag
    19681 tcgacgccca tcgcccgcat tgcacgtacc atcaccacgt ttttcgactg acccagcccc
    19741 tgacgtaagc gaattggacc agcatactgc ggtggtgagt tcttcggctg ccagtcagaa
    19801 ccggcacctg catcccagcg agaaattggc acatcgttca acatacttgc cagcgtcaga
    19861 cctttatcca tcgccgcggt gtagaggaac ggtttgatgt tggaacccac ctgacgcagt
    19921 gcctgggtgg cgcggttaaa cttgctctga ttgaaatcaa agccaccgac cagcgccata
    19981 acggcaccat tttgcggatt gatcgacacc agcgccgagt tcacttccgg cacttgtgcc
    20041 agccaccatg catcgccaac ctgacgaacc cagatttgct gacccgtttg cagaacatcg
    20101 gtcactttac gcggcgtcgg tccctgctga gtatccgaac ggtaaggacg cgcccagcga
    20161 acgccttcca tactcaatgc gacggtcgac ccgtccgcca gcatcgccgt cgctccctga
    20221 ggattggcgc tggtgactgc ggcaggcagc agcggaccat aggtcggcag cgccttcagc
    20281 gtatcggtaa tcttgttgtt atcccacgcc gactcgccca ctttccacag cacatttgcc
    20341 gggccgcgat agccgtggcg catgtcgtag tccagcacgt tattacgtac cgcctgctgc
    20401 gcggcctgct gcactttgcg ggtgatggtg gtgtaaatgc gataaccgtc ttcataggca
    20461 ctttcgccat aacggttata catctcctgg cgcaccattt cgctcaggta cggcgcagag
    20521 aaagcaatct ccggcgcgtg ataattagcg ttaatcgcct cagtgcgtgt ctgatcgaac
    20581 tgttgctggg tgatataccc ttcatccagc atccgcgaca gtacgacgtt acgccgcgcg
    20641 acggcacgat ccattgagta gagcggatta aaggtggaag gcgctttcgg caacccggct
    20701 atcaccgcca tttcgttcag cgtcagttgg tcgaccgttt taccgaaata gacttgtgcc
    20761 gcagcaccga caccataggc gcggtaacca aggtaaatct tgttcagata aagctcgagg
    20821 atttcgtctt tcgtcaacaa ctgttcaatg cgaatcgcga ggaagacttc cttaatctta
    20881 cgcatcatcg tgcgttctgg actgaggaag aaatttctcg ccagctgctg ggtaatggta
    20941 ctcgcccctt gtgacgcgtg gccggagaac agcgccacac ttgccgcacg gaagatcccc
    21001 accgggtcaa cgccgtgatg ctcgtagaag cggctgtctt ccgtcgcgat aaaggctttc
    21061 accatctccg gtgggatttg atccaacgta accggaatac gacgtttctc accgtattga
    21121 gcaatcagct cgccatcggc gctgtaaatc tgcatcggaa tttgcaggcg aacatctttt
    21181 aatgtcacca catccggcag ttgtggctcg atgtagcggt ataggccata aatcgagcct
    21241 gctcccagca gaatgcaaca gactgcaagg atcaaaaaat actttacgaa cttcactgga
    21301 aatttcccat ttagtttcat ttgggcagtt tataaacaaa cgcgcggtag tataaaggca
    21361 agccagacgc attgatatac ccgtcagagt gacgggtgat aaggagatca tcacaatgac
    21421 atttaagatc tggcaaattg gtttgcattt acaacagcaa gaagcggcag cggttgcgat
    21481 cgtgcggggc gcaaaagaat gctttttgca acgctggtgg cggttgccgc tggcgcacga
    21541 cattatcaaa gatgggcgga ttgttgatgc gcagcagctg gctaaaacgt tgttaccgtg
    21601 gagtcgcgaa ctgccgcagc gtcatcacat tatgttggcg tttcccgcca gtcgcacatt
    21661 acagcggtca tttccgcgcc cgtcgatgtc ccttggtgag cgggagcaaa cggcctggct
    21721 gtcagggacg atggcccgcg agctggatat ggatccggac tccctgcgct tcgattacag
    21781 cgaagactca ctcagccccg cttataacgt gactgccgcg caaagcaaag agctggcaac
    21841 gctgcttacg ctggcagaaa ggttgcgtgt tcatgttagt gcgatcaccc cggatgccag
    21901 tgcattacag cgattcctgc cttttttacc ttctcatcag caatgtctgg cctggcgtga
    21961 taacgaacag tggctgtggg cgacgcgcta tagctggggg cgcaaactgg cggtggggat
    22021 gactagcgcg aaggagctgg cggcagcgtt atccgttgat cccgacagcg tcgcgatatg
    22081 tggcgaaggc ggatttgacc cctgggaggc cgtttctgtt cgtcagccgc cgctaccgcc
    22141 gcctggtgga gactttgcca tcgcgctggg gctggcgctt gggaaggcgt actgatgaat
    22201 ccgccaatta attttttgcc ctggcgacag caacgccgga ccgcttttct gcgtttctgg
    22261 ttgctgatgt tcgttgcgcc tctgctgctg gccgtcggga taacgctaat actgcgtctg
    22321 acaggcagcg ccgaagcgcg catagacgca gttttgcttc aggcggaaca acaactcgcc
    22381 cgcagcttac agataacgaa gccacgtttg ctggagcaac agcagttacg cgaacagcgt
    22441 tcgcaacggc agcgccagcg acaatttacc cgcgactggc aatctgcgct ggaagcactg
    22501 gcggcgcttt tacccgagca cgcctggctg acaacgataa gctggcagca gggaacgctg
    22561 gagatcaagg ggctgacaac aagcattacc gcgttaaacg cactagaaac gtcactccgc
    22621 caggatgcgt cttttcatct caatcagcgg ggagccacgc agcaggatgc gcagggacgc
    22681 tggcaatttg agtatcagtt aacaaggaag gttagcgatg aacatgttct ttgactggtg
    22741 gttcgccaca tcaccccgcc tccgccagtt ttgctgggca ttctggttgc tgatgttagt
    22801 tacgctcatt tttctatcat cgacacacca tgaagagcgc gacgcattaa ttcgactacg
    22861 ggcaagtcat caccagcagt gggccgcact gtatcgcctg gtagacacca ctcccttcag
    22921 cgaggaaaaa acgctgccct tttcgccact ggattttcag ttatccggcg cgcaactggt
    22981 ttcctggcat ccatccgcgc agggaggtga gttggcgttg aaaacgctgt gggaagcagt
    23041 gccgtcggca tttacacggc tggcagagcg caacgtcagc gtgagccgtt tttcgttaag
    23101 cgtggaaggt gatgatcttt tgttcacgct acaactggag atgccgcatg agggttaaac
    23161 gctggttgtt ggcaggtatt gcattgtgcc ttttaaccgg tatgcgtgac ccttttaaat
    23221 cgccggaaga tctatatgcc ggattagcga acttagccag tggcgctatc aggggatggt
    23281 agggcgaggc gagcgcatca tcggtgtaat aaaagacggg caaaagaaat ggcgacgggt
    23341 gcagcaaaac gatgtgctgg aaaacggctg gacaatttta cagctgacgc cagacgtact
    23401 aacgctgggt accgggacaa actgcgaacc gccacaatgg ttgtggcaac ggcaaggaga
    23461 tacaaatgaa gcaatggata gccgcactac tgttgatgct gatacccggc gtacaggcgg
    23521 caaagccgca aaaagtgacg ctgatggtgg atgacgttcc ggtagctcag gtgttgcagg
    23581 cgctggctga acaggagaag ttgaacctgg tcgtgtcgcc agacgtcagc ggtacggtgt
    23641 cgttacatct aacagatgtt ccctggaagc aggcactaca aactgtagtg aaaagcgccg
    23701 gactgataac gcggcaggaa ggcaacattc tctcagtgca ttccattgcc tggcagaatg
    23761 acaatatcgc ccgccaggag gcggagcagg cgcgggcgca ggcaaatctg ccgctggaaa
    23821 atcgcagtat aaccctgcaa tacgccgacg cgggagaact ggcgaaagcg ggggagaagc
    23881 tactgagtgc caaagggagt atgaccgtcg ataaacgcac caatcgcctt ttgctacgag
    23941 ataacaaaac ggcgttaagc gcgcttgaac agtgggtagc gcaaatggat ctgccggtcg
    24001 ggcaggttga gctgtcggcg catattgtca ccattaatga aaaaagtttg cgtgagttag
    24061 gcgtgaaatg gacgctggcc gatgcgcaac acgctggtgg cgttgggcaa gtcaccacgc
    24121 ttggtagcga cctctccgta gcgacggcga caacgcatgt cggttttaac attgggcgca
    24181 tcaacggacg cttgctggat cttgagcttt ccgcgctcga acaaaaacag cagctggata
    24241 ttatcgccag tccgcgtctg ctggcctcac atcttcagcc tgccagcatt aaacaggaga
    24301 gcgaaattcc atatcaggtt tccagcgggg aaagtggcgc gacgtcggtg gaatttaaag
    24361 aggccgtcct ggggatggag gtcatgccca cggtgttaca aaaaggtcgc atccggctga
    24421 aattacacat cagccagaac gttccggggc aggtgctaca gcaggccgat ggcgaagtgc
    24481 tggcgattga taagcaggag atcgaaacgc aggtcgaggt aaaaagcgga gaaacgttgg
    24541 cgctgggcgg catttttacc cgtaaaaata aatcgggtca ggatagcgta ccgttgcttg
    24601 gcgacattcc ctggttcggg caattatttc gtcatgacgg aaaagaagat gaacgacgcg
    24661 agttagtggt gtttatcacg ccacgactgg tttccagtga gtaaacagcc gtaaaagcgg
    24721 taatgttttt atgctgaacg tgtttcatct atttgacgcg cgcaggtatt tagcatacaa
    24781 ggagtaccga tttgagagtt ggtgctcttc gctgcctgcg ttccatgatg atgatttatc
    24841 attcaggcgg cattttgctg tcttttttac gctaatctta cccggtgatt tatcgccaga
    24901 gcggtggtag caaggcagcg cgcttgcagc gaccagatat gcagagggat gggtgattta
    24961 ttcagttgcc aaacccgctg gagtattgag ataattttca gtctgactct cgcaatatct
    25021 tatgaggttt cagttcatgt cctgcggcgc tctctgagcg aagcgggttt atcattaacg
    25081 aatagtctta gtagtaccga aaaaatggca gagaaacgca atatctttct ggttgggcct
    25141 atgggtgccg gaaaaagcac tattgggcgc cagttagctc aacaactcaa tatggaattt
    25201 tacgattccg atcaagagat tgagaaacga accggagctg atgtgggctg ggttttcgat
    25261 ttagaaggcg aagaaggctt ccgcgatcgc gaagaaaagg tcatcaatga gttgaccgag
    25321 aaacagggta ttgtgctggc tactggcggc ggctctgtga aatcccgtga aacgcgtaac
    25381 cgtctttccg ctcgtggcgt tgtcgtttat cttgaaacga ccatcgaaaa gcaacttgca
    25441 cgcacgcagc gtgataaaaa acgcccgttg ctgcacgttg aaacaccgcc gcgtgaagtt
    25501 ctggaagcgt tggccaatga acgcaatccg ctgtatgaag agattgccga cgtgaccatt
    25561 cgtactgatg atcaaagcgc taaagtggtt gcaaaccaga ttattcacat gctggaaagc
    25621 aactaattct ggctttatat acactcgtct gcgggtacag taattaaggt ggatgtcgcg
    25681 ttatggagag gattgtcgtt actctcgggg aacgtagtta cccaattacc atcgcatctg
    25741 gtttgtttaa tgaaccagct tcattcttac cgctgaaatc gggcgagcag gtcatgttgg
    25801 tcaccagcga aaccctggct cctctgtatc tcgataaggt ccgcggcgta cttgaacagg
    25861 cgggtgttaa cgtcgatagc gttatcctcc ctgacggcga gcagtataaa agcctagctg
    25921 tactcgatac cgtctttacg gcgttgttac aaaagccgca tggtcgcgat actacgctgg
    25981 tggcgcttgg cggcggcgta gtgggcgatc tgaccggctt cgcggcggcg agttatcagc
    26041 gcggtgttcg tttcattcaa gtcccgacga cgttactgtc gcaggtcgat tcctccgttg
    26101 gcggcaaaac tgcggtcaac catcccctcg gtaaaaacat gattggcgcg ttctaccagc
    26161 ctgcttcagt ggtggtggat ctcgactgtc tgaaaacgct tcccccgcgt gagttagcgt
    26221 cggggctggc agaagtcatc aaatacggca ttattcttga cggtgcgttt tttaactggc
    26281 tggaagagaa tctggatgcg ttgttgcgtc tggacggtcc ggcaatggcg tactgtattc
    26341 gccgttgttg tgaactgaag gcagaagttg tcgccgccga cgagcgcgaa accgggttac
    26401 gtgctttact gaatctggga cacacctttg gtcatgccat tgaagctgaa atggggtatg
    26461 gcaattggtt acatggtgaa gcggtcgctg cgggtatggt gatggcggcg cggacgtcgg
    26521 aacgtctcgg gcagtttagt tctgccgaaa cgcagcgtat tataaccctg ctcacgcggg
    26581 ctgggttacc ggtcaatggg ccgcgcgaaa tgtccgcgca ggcgtattta ccgcatatgc
    26641 tgcgtgacaa gaaagtcctt gcgggagaga tgcgcttaat tcttccgttg gcaattggta
    26701 agagtgaagt tcgcagcggc gtttcgcacg agcttgttct taacgccatt gccgattgtc
    26761 aatcagcgta acaacaagaa aggtcaggcc gcttatcaag cggtctatta gcttcaggtt
    26821 aattgcaacg tggtaagcat taacctttta gtggggtgtt aaatggatga attcaaacca
    26881 gaagacgagc tgaaacccga tcccagcgat cgtcgtactg gtcgttctcg tcaatcttct
    26941 gaacgttctg agcgtactga acgtggcgaa ccgcagatca attttgatga tattgaactt
    27001 gatgacactg acgatcgccg tccgactcgt gcgcaaaaag agcgcaatga ggaaccggaa
    27061 atcgaagaag aaattgacga atccgaagat gaaaccgtgg atgaagagcg cgtagagcgt
    27121 cgtccgcgta agcgcaaaaa agcagccagt aaacccgctt ctcgtcagta tatgatgatg
    27181 ggcgtcggca ttctggttct actgctgttg atcatcggta tcggttctgc gctaaaagcc
    27241 ccctcgacca cttccagcga tcaaaccgcg tctggcgaga agagtattga tcttgctggc
    27301 aatgcgaccg atcaggcgaa tggcgtgcag ccagcgccgg gaaccacgtc tgcggaaaat
    27361 actcagcagg atgtttctct gccaccgatc tcttctacgc cgactcaagg gcaaaccccg
    27421 gtggcaacgg atggtcaaca acgtgttgaa gtgcagggtg acctgaacaa tgcgctgacc
    27481 cagccacaaa atcagcaaca gctgaacaat gtggcggtca attccacgtt gccgactgaa
    27541 cccgcaacgg ttgcgcctgt tcgcaatggc aatgcatcgc gtgacacggc gaaaacgcaa
    27601 accgctgaac gtccgtccac tacgcgccca gttcgtcagc aggcggtgat tgaaccgaaa
    27661 aaaccgcaag caaccgtgaa aacggagccg aagccggtag cacagacgcc gaagcgtact
    27721 gaacctgaac cagctgcccc tgtggcgagc acgaaggcac cggctgcgac ttctacgcca
    27781 gcaccaaaag agacggcgac tacggctcca gtacagacgg catccccggc gcaaaccacg
    27841 gcaacaccag ccgctggagg gaagaccgca ggtaatgttg gttcgttgaa atcggcaccg
    27901 tccagccatt acactctgca gctgagcagt tcctctaact acgacaacct gaacggttgg
    27961 gcgaagaaag agaatctgaa aaactacgtt gtctatgaaa cgacgcgtaa tggtcagccg
    28021 tggtatgtcc tggtttctgg cgtgtacgct tcgaaagaag aggcgaaaaa agcggtatct
    28081 acattgccag cagatgttca ggccaaaaac ccgtgggcga aaccgctgcg tcaggtacag
    28141 gccgatctga agtaatcaag gttatctccc gcaatggttt atcgttgcgg gagctgcctg
    28201 aagcgctgga tgctgtcgga gctttctcca cagccggaga aggtgtaatt agttaatcgg
    28261 catgaagaaa aatcgcgctt ttttgaagtg ggcagggggc aagtatcccc tgcttgatga
    28321 tattaaacgg catttgccca agggcgaatg tctggttgag ccttttgtag gtgccgggtc
    28381 ggtgtttctc aacaccgact tttctcgtta tatccttgcc gatatcaata gcgacctgat
    28441 cagtctctat aacattgtga agatgcgtac tgatgagtac gtacaggccg cacgcgagct
    28501 gtttgttccc gaaacaaatt gcgccgaggt ttactatcag ttccgcgaag agttcaacaa
    28561 aagccaggat ccgttccgtc gggcggtact gtttttatat ttgaaccgct acggttacaa
    28621 cggcctgtgt cgttacaatc tgcgcggtga gtttaacgtg ccgtttggcc gctacaaaaa
    28681 accctatttc ccggaagcag agttgtatca cttcgctgaa aaagcgcaga atgccttttt
    28741 ctattgtgag tcttacgccg atagcatgga gcgcgcagat gatgcatccg tcgtctattg
    28801 cgatccgcct tatgcaccgc tgtctgcgac cgccaacttt acggcgtatc acacaaacag
    28861 ttttacgctt gaacaacaag cgcatctggc ggagatcgcc gaaggtctgg ttgagcgcca
    28921 tattccagtg ctgatctcca atcacgatac gatgttaacg cgtgagtggt atcagcgcgc
    28981 aaaattgcat gtcgtcaaag ttcgacgcag tataagcagc aacggcggca cacgtaaaaa
    29041 ggtggacgaa ctgctggctt tgtacaaacc aggagtcgtt tcacccgcga aaaaataatt
    29101 ctcaaggaga agcggatgaa acagtatttg attgccccct caattctgtc ggctgatttt
    29161 gcccgcctgg gtgaagatac cgcaaaagcc ctggcagctg gcgctgatgt cgtgcatttt
    29221 gacgtcatgg ataaccacta tgttcccaat ctgacgattg ggccaatggt gctgaaatcc
    29281 ttgcgtaact acggcattac cgcccctatc gacgtacatc tgatggtgaa acccgtcgat
    29341 cgcattgtgc ctgatttcgc tgccgctggt gccagcatca ttacctttca tccagaagcc
    29401 tccgagcatg ttgaccgcac gctgcaactg attaaagaaa atggctgtaa agcgggtctg
    29461 gtatttaacc cggcgacacc tctgagctat ctggattacg tgatggataa gctggatgtg
    29521 atcctgctga tgtccgtcaa ccctggtttc ggcggtcagt ctttcattcc tcaaacactg
    29581 gataaactgc gcgaagtacg tcgccgtatc gacgagtctg gctttgacat tcgtctggaa
    29641 gtggacggtg gcgtgaaggt gaacaacatt ggcgaaatcg ctgcggcggg cgcggatatg
    29701 ttcgtcgccg gttcggcaat cttcgaccag ccagactata aaaaagtcat tgatgaaatg
    29761 cgcagtgaac tggcaaaggt aagtcatgaa taagtttgaa gatattcgcg gcgtcgcttt
    29821 tgatcttgat ggtacgctgg tcgacagtgc tcctggtctt gctgctgcgg tagatatggc
    29881 gctgtatgcg ctggagttgc ccgtcgcagg tgaagaacgc gttattacct ggattggtaa
    29941 cggcgcagat gttctgatgg agcgcgcatt gacctgggcg cgtcaggaac gtgcgactct
    30001 gcgtaaaaca atggggaaac cgcccgttga tgacgacatt ccggcagaag aacaggtacg
    30061 tattctgcgt aaactgttcg atcgctacta tggcgaggtt gccgaagagg ggacgttttt
    30121 gttcccgcac gttgccgata cgttgggcgc gttgcaggct aaaggcctgc cgctaggctt
    30181 ggtcaccaac aaaccgacgc cgttcgtcgc gccgctgctc gaagccttag atattgccaa
    30241 atactttagc gtggttatcg gcggcgatga tgtgcaaaac aaaaaaccgc atccggaccc
    30301 gctgttactg gtggctgagc ggatgggaat tgccccacaa cagatgctgt ttgtcggcga
    30361 ctcacggaat gatattcagg cggcaaaagc ggcaggttgc ccatcagttg gcttaaccta
    30421 cggatacaac tacggcgagg ctatcgatct cagccagcct gatgtaattt atcagtctat
    30481 aaatgacctt ctgcccgcat tagggcttcc gcatagcgaa aatcaggaat cgaaaaatga
    30541 ctaagcccat cgtttttagt ggcgcacagc cctcaggtga attgaccatt ggtaactaca
    30601 tgggtgcgct gcgtcagtgg gtaaacatgc aggatgacta ccattgcatt tactgtatcg
    30661 ttgaccaaca cgcgatcacc gtgcgccagg atgcacagaa gctgcgtaaa gcgacgttgg
    30721 atacgctggc cttgtatctg gcttgtggta tcgatcctga gaaaagcacc atttttgttc
    30781 agtcccacgt accagaacat gcgcagttag gctgggcact gaactgctat acctacttcg
    30841 gcgaactgag tcgcatgacg cagtttaaag ataaatctgc gcgttatgcc gagaacatca
    30901 acgctggtct gtttggctat ccggtgctga tggctgcgga catcctgctg tatcaaacta
    30961 atctggtacc ggtgggtgaa gaccagaaac agcacctgga actgagtcgc gatatcgccc
    31021 agcgtttcaa cgcgctgtat ggcgagatct ttaaggtgcc ggagccgttt attccgaaat
    31081 ctggcgcgcg cgtaatgtcg ctgctggagc cgaccaagaa gatgtccaag tctgacgata
    31141 accgcaataa cgttatcggc ctgctggaag atccgaaatc ggtagtgaag aaaatcaaac
    31201 gcgcggtcac tgactccgac gagccgccgg tagttcgcta cgatgtgcag aacaaagcgg
    31261 gcgtttccaa cctgctggat atcctttctg cggtaacggg ccagagcatc ccagaactgg
    31321 aaaaacagtt cgaaggcaag atgtatggtc atctgaaagg tgaagtggct gatgccgttt
    31381 ccggtatgct gactgaattg caggaacgct atcaccgttt ccgcaacgat gaagccttcc
    31441 tgcaacaggt gatgaaagac ggcgcagaaa aagccagtgt acacgcttcc cgtacgctga
    31501 aagcggtgta cgaagcgatt ggttttgtgg cgaagccgta atatcaaaac gctcggacgg
    31561 tcccctcgcc cctttgggga gagggttagg gtgaggggag caggcaagcg ccagtgggaa
    31621 ttaccctttg ctcgcattgt cgaataaatc acttcgtcat cactctgaaa ccggggaaac
    31681 ccggtttttt tatcctcttt tcgcaacaac ccgaaaaaat gtgaagcgcc tcgccgtttc
    31741 cacatctcgt cgtcgccact tatcttcatt ttaatgaaga tataaatatt cattttattg
    31801 aaaatatatt atgcgtcgaa cgtttatcaa aaaagaaggc gtcgtcatca cgacactggc
    31861 ccgttatttg ttgggtgaaa aatgcggtaa tcgactgaaa accatagatg agctggcaac
    31921 tgaatgccgt tcatcggttg gcctgacgca ggccgcgttg aaaacgctgg aatcaagcgg
    31981 agcgatacgg attgaacgcc gtgggcgcaa tggcagttat cttgtcgaga tggataacaa
    32041 agcattgctg agtcatgtgg atatcaacaa cgtggtatgt gcgatgcctt tgccctatac
    32101 ccgcttgtac gaaggtttag cgagcggatt gaaagcccag tttgatggca ttccttttta
    32161 ctatgcgcat atgcgcggcg cggatattcg cgtggagtgt ttacttaacg gtgtctatga
    32221 catggcagta gtttcccgcc tggcggcgga aagttatctc agccaaaaca atttgtgcat
    32281 cgccctcgag cttggcccgc atacgtatgt tggtgaacat caactgatat gccgcaaagg
    32341 tgagtctgga aatgttaagc gagtaggtct cgatagtcgt tcggcggatc agaaaatcat
    32401 gaccgatgtc ttttttggcg atagtgatgt ggaacgagtc gatctctctt atcacgagag
    32461 tttacaacgc attgttaaag gcgatgtcga tgcggtgatc tggaacgtgg tggcggaaaa
    32521 cgaactgacc atgctgggat tagaggcgac gccgctaaca gacgatccgc gatttttaca
    32581 ggccaccgaa gccgtggtcc tgacgcgagt cgatgattac ccaatgcaac aattgctgcg
    32641 tgctgttgta gataaacacg ccctgcttgc ccatcaacaa cgggtggtga gtggggaaca
    32701 ggaacccagt tattaaacga aaggcatctg atatggaaac cagactcaac ctgctttgcg
    32761 aggcaggcgt tattgataag gacgtctgca aaggcatgat gcaggtcgtc aacgtactgg
    32821 aaaaagagtg ccatctgccg gtgcgcagtg agcaaggaac gatggcgatg acacatatgg
    32881 cgagtgcact gatgcgcagt cgccgtggtg aagaaataga gccgctggat aacgagttgc
    32941 tggcggaact ggcgcaatcc agccactggc aagccgttgt gcaattgcat caggagttgt
    33001 tgaaggaatt cgctctggaa gttaacccgt gtgaagaagg ctattttctg gccaatcttt
    33061 atggattatg gatggctgct aacgaagagg tttgaagctc ccgacaaatc cacgtcgcac
    33121 aacctaaata ttcaaaaaaa tgaatattta attcaaggaa cagaaacgag gcaacaagat
    33181 gtttgtagaa gcattgaaac gccagaaccc ggcgctgatt tccgccgcac taagtttgtg
    33241 gcagcagggc aaaatcgccc cggacagctg ggtgatcgac gtggatcaga tactggaaaa
    33301 cggtaagcgg ctcattgaga cggcgcggct ttacggcatt gaactgtatc tgatgaccaa
    33361 gcaatttggt cgtaatccgt ggctggcgga aaaattgctg gcattaggct acagcggcat
    33421 tgtggcggtg gattacaaag aggcgcgagt catgcgccgc gctggtttgc cagtggcgca
    33481 tcaggggcat ctggtacaaa tcccttgtca tcaggttgct gacgccgttg aacagggcac
    33541 cgacatcatc accgtgttta ctctcgacaa agcgcgggaa gtttctgcgg cagcggttaa
    33601 ggccggacgg gtccagcctg tgctgctcaa agtttatagc gacgatgatt ttctttatcc
    33661 ggggcaggag agcggttttg tacagcattc gctgcacgag gtgattgctg aaattaagaa
    33721 actgccgggg ctgcatttag ccggactaac ccatttccct tgcctgcttt gggatgaggc
    33781 tgctgggaaa gttttgccaa caccgaatct tcacacgctg gtacaagcac gggatcaact
    33841 ggcgaaatct ggtattgcga ttgagcaact gaacgtacct tcagcgacca gctgcacttc
    33901 gctgccattg ctggcggaat acggtgcgac tcatgccgaa cccggtcatg cgctgaccgg
    33961 tactattccg gcaaaccagc agggcgatca gcctgaacgt atcgcgatgc tctggttaag
    34021 tgaaatctcc catcatttcc gtggcgacag ctactgctac ggcggcggtt actaccgtcg
    34081 tggtcatgcg caacatgcgc tggtgtttac gccagaaaat caaaagatta ctgaaaccaa
    34141 tctcaaaact gtggatgaca gcagtatcga ctacaccctg ccgctggcag gcgagtatcc
    34201 ggtgagcagt gcagtggtgc tctgttttcg cacgcagatt tttgtcaccc gtagcgatgt
    34261 ggtgctggtg tccggtattc agcgcggcga accagaaata gttggtcgtt atgacagcct
    34321 cggcaaccct ctggaggcat aaatggcgcg atttgtggtg ttagtgattg atagctttgg
    34381 cgtaggggca atgaaagatg tcacgctggt gcgcccgcaa gatgccgggg cgaatacctg
    34441 tggtcacatc ctgagccagt tgccgcattt gcagctacca acgctggaga agctggggct
    34501 aatcaacaca ttgggttatg cgccaggcga tatgcagccg tcagattccg caacctgggg
    34561 cgtggcagag ctgcaacatg aaggtggcga tacctttatg gggcatcagg aaattttagg
    34621 cacgcgcccg ttaccgccgc tgcggatgcc ttttcgcgat gtgattgacc gtgttgagca
    34681 ggcattagtt tccgctggct ggcaggtgga gcgccgtggc gatgatctgc aatttctgtc
    34741 ggtcaatcag gcggttgcga ttggcgataa tctcgaggcg gatttaggcc aagtctataa
    34801 cattaccgcc aatctctctg tgatctcttt tgacgacgca atcaaaattg gtcgtatcgt
    34861 gcgtgaccag gtacaggtcg gtcgggtcat tacatttggc ggcctgttaa ccaacagtca
    34921 gcgtattctc gatgccgcag aaagcaaaga agggcgcttt attggtatta atgcgccgcg
    34981 ttctggcgct tatgacaacg gcttccaggt cgtgcatatg ggctacggtg ttgatgaaaa
    35041 agtgcaggtg ccgcaaaaac tgtatgaagc gggcgtgcca accgtgctgg tgggtaaggt
    35101 ggcagatatc gtcagcaatc cttatggtgt gagctggcaa aacctggtgg atagccagcg
    35161 gattatggat atcaccctcg acgaatttaa cacccatccg acggcgttta tttgcatcaa
    35221 tatccaggaa accgacctcg ctggtcatgc agaagacgtc gcacgttatg ccgaacgttt
    35281 gcaggtcgtt gaccgtaacc ttgcccggct tgttgaggcg atgcagccag atgattgcct
    35341 ggtcgtgatg gcggatcacg gtaacgatcc gaccattggt cacagccacc atacccgtga
    35401 agtggtgcca gtgctggttt atcagcaagg gctggtcgcc acgcagctcg gtgtgcgcac
    35461 cacgctttct gatgtgggag ctaccgtgtg tgaatttttc cgtgcgccac cgccacaaaa
    35521 tggtcgctct tttctttcct ccctccggtt tgcaggagac accctatgag ttttgatccg
    35581 acgggttaca ccctggccca tgagcatctg catattgatc tctccggctt taaaaacaac
    35641 gtggactgcc gccttgatca gtatgcgttc atttgccagg agatgaacga cctgatgacc
    35701 cggggtgtac gtaatgtgat tgagatgacc aaccgttaca tggggcgcaa tgcgcaattt
    35761 atgctcgatg taatgcgcga aaccgggatc aacgtggtgg cctgtactgg ttattaccag
    35821 gatgcgtttt tcccggaaca tgtggtgacc cgcagcgtgc aggaactggc gcaggagatg
    35881 gtcgatgaaa ttgaacaggg tatcgatggc acggagctga aagccgggat catcgcggag
    35941 atcggctcca gcgaaggaaa gattacgccg ctggaagaga aggtatttat tgccgctgcg
    36001 ctggcgcata accagaccgg acgcccgatc tccacgcata cgtcgttcag caccatgggg
    36061 ctggaacaac tggctctgtt gcaagcccac ggagttgatc tttcgcgcgt caccgttggt
    36121 cactgcgatc tgaaagacaa cctcgacaac attttgaaga tgatcgatct cggcgcgtac
    36181 gtgcagttcg acaccattgg taagaacagt tactacccgg acgaaaagcg cattgcgatg
    36241 ctccacgcgc tacgtgaccg tgggttgctg aaccgcgtca tgctgtcgat ggatattacg
    36301 cgccgctccc atttaaaagc caatggtggt tatggctatg actatttatt gaccaccttt
    36361 attccgcaat tgcgccagtc aggattcagt caggccgatg tggatgtgat gttacgtgaa
    36421 aatccctctc aatttttcca ataaggacag actcatgaaa aagattggcg ttgcaggctt
    36481 acagcgtgag cagattaaaa aaaccattga agcgacggct cctggctgtt ttgaagtttt
    36541 cattcacaac gacatggaag cggcaatgaa ggtgaaatcc gggcaactag attattacat
    36601 tggcgcgtgt aataccggtg cgggcgcggc attgtcgatt gccatcgcgg tgattggcta
    36661 taacaaaagt tgcaccattg ccaaaccagg cattaaagcg aaagacgagc atatcgccaa
    36721 aatgatcgct gaagggaaag tggcgtttgg cctttccgtt gagcacgtcg aacacgcgat
    36781 tccgatgttg attaaccatc tgaaataaaa ggcacgacta tggatctgta tattcagatt
    36841 atcgtggtgg cgtgcctgac gggtatgaca tcgcttctgg agcatcgctc ggcggctgtt
    36901 tttcatgacg gcatccaccc gatcctgccg caactgattg aaggctatat gaaccgtcgc
    36961 gaggcgggga gtatcgcttt tggtctgagc attggttttg tggcctcggt ggggatctct
    37021 tttaccctga aaaccgggct gctcaacgca tggttactct ttcttcctac cgatatcctc
    37081 ggcgtactgg cgataaacag cctgatggcg tttggtcttg gcgctatctg gggcgtgttg
    37141 atccttactt gcctgttgcc agttaaccag ctgctgaccg cgctgccggt ggatgtatta
    37201 ggtagcctcg gggaattaag ctcgccggtg gtttctgctt ttgcactgtt cccgctggtg
    37261 gcgattttct accagtttgg ctggaagcaa agtctggtcg ccgccgtggt tgttctgatg
    37321 acccgtgtgg tagtcgtgcg ctatttccca catcttaacc ctgaatccat cgaaatcttt
    37381 attggcatgg tgatgctgct ggggatcgcg ataactcacg acctgcgtca tcgtgatgaa
    37441 aatgacatcg atgccagcgg gctttcggtg tttgaagagc gcacgtcgcg gattatcaaa
    37501 aacttacctt atatcgccat cgtgggagca ttgattgccg ccgttgccag tatgaagatt
    37561 tttgctggta gcgaagtttc gatcttcaca ctggagaaag cgtattccgc aggcgtgacg
    37621 ccggaacaat cgcaaacgct gattaatcag gcggctctgg cagaatttat gcgcggactg
    37681 gggtttgtgc cgttgattgc caccaccgcc ttagcaacgg gtgtgtatgc agttgcgggc
    37741 tttacctttg tttatgcggt gggctatctc tcgccgaatc cgatggttgc agcggtatta
    37801 ggcgcagtgg ttatttcggc ggaagtcttg ctgcttcgtt cgatcggcaa atggctggga
    37861 cgctacccgt cggtgcgtaa tgcgtcggat aacatccgta acgccatgaa tatgctgatg
    37921 gaagtggcgc tgctggtcgg ttcgattttc gcagcaatta agatggcggg ttataccgga
    37981 ttctctatcg cggttgccat ttacttcctc aacgaatccc tgggccgtcc ggtacagaaa
    38041 atggcggcac cggtcgtggc agtaatgatc accggtattc tgctgaatgt tctttactgg
    38101 cttggcctgt tcgttccggc ttaaggaagg cacctatgaa gacgtttcct ttgcaaagcc
    38161 tgactcttgc tgaagcgcag caaaagcagt ttgcgctggt ggacactatt tgccgccatt
    38221 ttccaggcag cgagtttctt gctggcggtg atttagggtt aactccaagt ctgaatcaac
    38281 cgcgtattac ccaacgggtg gagcaggtgc tggctgatgc atttcacgca caggccgcgg
    38341 cgctggtgca gggcgcgggg actggcgcga ttcgcgccgg gctggcggct ttgctcaaac
    38401 cggggcagcg tcttctggtg catgacgcgc ctgtttaccc gacgacacgg gttattattg
    38461 agcagatggg gctgacgctt attactgctg atttcaatga cctgtcggca ctgaagcagg
    38521 tcgtcgacga gcaacaacct gatgccgcgc tggtgcagca tacgcgccag cagccgcagg
    38581 acagctatgt gctggcagat gtgctggcaa cgttgcgcgc ggcaggtgtt ccagcgttaa
    38641 ccgatgataa ctatgcggtg atgaaggtag cgcgaatcgg ctgtgaatgc ggcgcgaatg
    38701 tctcgacatt ctcctgcttc aagctgtttg ggccggaggg cgttggtgca gtggttggcg
    38761 atgccgatgt tattagtcgg attcgcgcca cgctttactc cggcggtagc cagatccagg
    38821 gctcacaggc gctggaagtc ttgcgtggtc tggtgttcgc gccagtgatg catgcggtgc
    38881 aggcgggagt atccgaacgg ttgctggctt tgcttaacgg cggcgcggtg gcggaagtga
    38941 aaagcgccgt cattgccaat gcgcagtcga aggtgttgat tgtcgagttt catcagctga
    39001 ttgccgccag ggtgctggaa gaagcgcaaa agcgtggcgc gttgccttac ccggtcggtg
    39061 cagagtcgaa atatgaaatc ccgccgctct tctatcgact ttcaggaacg tttcgccagg
    39121 cgaatccaca actcgaacat tgtgcgattc gcattaaccc gaatcgcagc ggtgaagaga
    39181 cggtgctgcg gattttgcgt gagagtattg cgagtattta atgctggata gcaccgttcg
    39241 gcaatcaagc taaattccgc actgaacggt cccctcgccc ctttggggag agggttaggg
    39301 tgaggggaac gtgcgggatg taaaattcag tgcgccagca tcgccggatg cgatgctggc
    39361 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    39421 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    39481 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39541 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39601 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39661 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39721 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39781 acccgttcct gttcgatcac tttctctttc gtcgacttgc cttgtgagac gccaaaatcg
    39841 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39901 tttccctgcc agcggatcag tacgccgtct gccactaagt cgctgatggc tttgcgaatg
    39961 gtaatgcggc tgacgttata ttgtgtacaa agctcgtttt cggtagggat ctgttgcccg
    40021 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    40081 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    40141 ctcggattat agggtcgttt tccggcaagt aacagcacgt tggcgtgaag atgctcacgc
    40201 caacgttata cctaccaggc accgtggtac tgaatggttt tcgccgcgca cgccgttccc
    40261 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    40321 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    40381 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    40441 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    40501 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40561 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40621 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40681 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40741 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40801 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40861 caggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40921 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40981 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    41041 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtagagtaac
    41101 cctcatagcc ccgatcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    41161 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgtaatt
    41221 tatcgcccag tttgtcgaaa taactcatca ccggttccgc ctggacatac ggcgcgcaaa
    41281 tgtcgaccat gctgaacagg cgcggcgaag gcaccagcgc cagcgcatga agcacatcat
    41341 tagcattaca cacgacgttc gattcatacg gcgttaacgg ttcgaggatc agatccatgc
    41401 cgatgttttc cgcgtactca cacagttcgc ttaagttctc tgccagccgc ccccagataa
    41461 cgttaggcgg cgtgagatag cccgcgtggg ccgcagaaat cagcgtataa cccgcgttca
    41521 tctcttttgc catatctatc gccagcttga tcatgtccag gctttcacga cgcatatgtt
    41581 catcgcccag catcatgtta tacggatagc cgttggtttc tggcgtgtaa ccgataatcg
    41641 gcatctgata cgtctgcgcc agcgccttga tttgtttgat gccgcccgct tttaagtccg
    41701 gcgcgaacgc gtgcgggcga ccgccccaaa tttcgatgcc gtcgtaaccc agctcgcttg
    41761 catcacgaaa tgcgtgttca ataggcagcc gctggtggcc gcaggtaaac atacctgttt
    41821 tcatcgggta actccttgcg taaatccggc ccgttgccgg gccgggagag ggattaatat
    41881 tccaccagac caccgtaata gcggcgttca tccgggttgt gatctttgta aatagacagg
    41941 tagtagcaga gccactccat tggcacgaac atcaggaacg gtgccagcca cgggtgcagc
    42001 ccttgcgaaa tttcggcgta atcgatgacg atcacgttgt cagtacgctg tttaacaaag
    42061 ttaatggcgc gttcggtggt gtggcgactt tcatcattgc cgagcaggaa caggaacgga
    42121 acgcccggtt cgacaatctc cagcgggcca tggcggaact ctccgctctc aatcacgcag
    42181 ccgtgcgtcc aggtaaattc catcagcgtt acaatgcctt ctttgtaacc cagcggacgc
    42241 agcggacccg cagcaacggt ataaatcatc ggccactggc tggccagttc accaagctgg
    42301 cggccttttt cttcccaggt gcgtaccaga tgaccgagcg cattcggcaa ctgcttgaga
    42361 tcgtttttga tcttgccgat ttccgcgttc ggcgcgaggc gggtgatcat ctccagcacc
    42421 acgctgtagc agagcagcag gtgaatttcc cagatacagt cggcctgata atcaatgcta
    42481 aattccgccg ccgaggtaat cgggctgtcc gcgcgtttgg tgaacgccgc agtgagtgct
    42541 ccgcaggccc ggcccagctc cagcgctttg attacctctt ccgttttacc gtagtcagaa
    42601 acgccaatca ccgcgcaacg atcgtcgagg cgatacgggg tgttatcgca gaactcccag
    42661 ccggaaatgg cgtagacctg aagatcggaa aagcgatccg ccagatgttt cgccgtttgc
    42721 gccgcgttga gtggcgagcc gcaggcaacg aaataaatac gatcaatgtc gcgcttcacc
    42781 atctcttcca cgatggcgtg caccagcgga acgtcatggc tgagaacttt ttccacttcc
    42841 tgaaccatat tttcggtgac cagaaagtcc acggtgcttt tatcaatatc caacatttta
    42901 ttttcttcag ataacattac gacaaagcgt tgagctggcg gctacgcttc gcccagaagg
    42961 cgtaagcagg cagaccagta gcaataacga tggcggcgca gataaggccg ggaatcggtg
    43021 cccagacaaa ggttgaggcg accagaatga ggcttgacgc aatggcgagg gtggtcatca
    43081 gcccgaaagc cggagtacgc cacagcggtt tgtaatcgtc gcgtttacga caccagatga
    43141 tggagccgaa ggtgagggta tttttgaaac acatcaccag ggtgaaataa cccagcaggc
    43201 tggtgagatc ggaaacgaag atgaagaaga tccccagcgc cccttgcagg atgatggaga
    43261 catccggcgt gttgtatttc ggatgcgcat ggccgaagca tttaaagaac aggttgtctt
    43321 tcgccatcgc gtattccagc cgcggctggt acatcacgca gctggaaagc gaaccaagaa
    43381 tgacgatcat cgccgtgatg gcaacaaaga gcccagcggt gctgccgagt gcggggatct
    43441 aggtcagggc gtcggaaatc ggcgtttcag aattggcgag tttgtcgaag ggcatcaggc
    43501 cggaaatcac cagcgccagc agggtgtaga gcaccagaac cagcagacag gaaccaatca
    43561 gcgctcgtgg catggttttt ccggggtttt taatttcgcc ggtcatataa cagatagagg
    43621 ccatgccggt atacgaccaa ctggtggcag agatccccgc cagcagcgcc ataaagctgc
    43681 ccgttgcgcc aatcgcagtg gtggtaaggg cggcaaaatt ctccgcttta aaccagaaga
    43741 tcccaaggcc aatgacgata gtgaacggga taattttggc gatggtaatt agcgtctgaa
    43801 acgctgcgcc gccttcaacg gagcgcaggt gtagcaacat aaaggcgata attaatccgg
    43861 cggcgataaa tttaccgagc aacggatcga taggcgttaa aaagccaaga ttgctgacaa
    43921 tcgccagcgc cataatcgac aatgacggcg catcgttggc ccagaagctg gcccagccgg
    43981 agaggaaagc cagcggtcgg cttccggcat ttttcagata aacataatct gcgccatttt
    44041 ccggataagc ggtggatagt tccgcataga cgcacatttg cgggatcaca attaacccgc
    44101 caatgacaaa cgcgagcacc gtaagccacg gcgtgcccgc tgcttttgcc acttcaccca
    44161 cagatacaaa aataccggag ccgacggttg tcccgacggc gattgcaaga acggcccaaa
    44221 atccgagctt gcgttggagt tcctggcttc ccatagcgat acctttttct tgtccaatac
    44281 gttgtagggg agagtttgca gccatttaaa aggtaacatg acaatgcatg atgaatataa
    44341 caacatatta tgttatgtga tgtggaggaa gatcacaaaa tttcgcacag gatcgcgctg
    44401 tggctaatgg atgtagttat aaaattgaat ttaaagtgaa aatattttta cgggcggggg
    44461 caagaaggac atagaaacaa atacgccctc ggaaaatcca gagggcgtcg ggcaattaaa
    44521 ccggtgttag ccgatttctg tcagagactt actgtgcagt aggaccgcaa ccgccaatga
    44581 ttttagaaat agaaattgcc gggtgcagca ggtagtcgta agagcagttc ttatcacggt
    44641 tttcaatatg accagtacat gcagttaacg tagccagtac tgcacctacc agtgcaactt
    44701 taa
//
LOCUS       DAZPEU010000014        44344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPEU010000014 DAZPEU010000000
VERSION     DAZPEU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.014"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1721
                     /gene="adeD"
                     /locus_tag="ACHU2L_000664"
     CDS             <1..1721
                     /gene="adeD"
                     /locus_tag="ACHU2L_000664"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR1355650.1"
                     /translation="YQELLAVSRGDAVADYIIDNVSILDLINAGEISGPIVIKGRYIA
                     GVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICD
                     PHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRD
                     HPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGLGGKELNAYIAAGIENC
                     HESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNSPQCMLCTDDRNPWEIA
                     HEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDAR
                     KVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQPVSASDFALQFTPGKRY
                     RVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNEGA
                     LAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMST
                     DTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFT
                     TLEVTE"
     gene            complement(1767..3158)
                     /gene="uhpT"
                     /locus_tag="ACHU2L_000665"
     CDS             complement(1767..3158)
                     /gene="uhpT"
                     /locus_tag="ACHU2L_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR1355651.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3296..4615)
                     /gene="uhpC"
                     /locus_tag="ACHU2L_000666"
     CDS             complement(3296..4615)
                     /gene="uhpC"
                     /locus_tag="ACHU2L_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR1355652.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4625..6127)
                     /gene="uhpB"
                     /locus_tag="ACHU2L_000667"
     CDS             complement(4625..6127)
                     /gene="uhpB"
                     /locus_tag="ACHU2L_000667"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR1355653.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6127..6717)
                     /gene="uhpA"
                     /locus_tag="ACHU2L_000668"
     CDS             complement(6127..6717)
                     /gene="uhpA"
                     /locus_tag="ACHU2L_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR1355654.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6792..7082)
                     /gene="ilvN"
                     /locus_tag="ACHU2L_000669"
     CDS             complement(6792..7082)
                     /gene="ilvN"
                     /locus_tag="ACHU2L_000669"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1355655.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7086..8774)
                     /gene="ilvB"
                     /locus_tag="ACHU2L_000670"
     CDS             complement(7086..8774)
                     /gene="ilvB"
                     /locus_tag="ACHU2L_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR1355656.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8880..8978)
                     /gene="ivbL"
                     /locus_tag="ACHU2L_000671"
     CDS             complement(8880..8978)
                     /gene="ivbL"
                     /locus_tag="ACHU2L_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR1355657.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9543..9632
                     /gene="tisB"
                     /locus_tag="ACHU2L_000672"
     CDS             9543..9632
                     /gene="tisB"
                     /locus_tag="ACHU2L_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR1355658.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9756..9830)
                     /gene="ysdE"
                     /locus_tag="ACHU2L_000673"
     CDS             complement(9756..9830)
                     /gene="ysdE"
                     /locus_tag="ACHU2L_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR1355659.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9912..11096
                     /gene="emrD"
                     /locus_tag="ACHU2L_000674"
     CDS             9912..11096
                     /gene="emrD"
                     /locus_tag="ACHU2L_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR1355660.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11104..11601)
                     /locus_tag="ACHU2L_000675"
     CDS             complement(11104..11601)
                     /locus_tag="ACHU2L_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR1355661.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11598..11960)
                     /locus_tag="ACHU2L_000676"
     CDS             complement(11598..11960)
                     /locus_tag="ACHU2L_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR1355662.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11950..12297)
                     /locus_tag="ACHU2L_000677"
     CDS             complement(11950..12297)
                     /locus_tag="ACHU2L_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR1355663.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12406..12819
                     /gene="yidI"
                     /locus_tag="ACHU2L_000678"
     CDS             12406..12819
                     /gene="yidI"
                     /locus_tag="ACHU2L_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR1355664.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12866..14308)
                     /locus_tag="ACHU2L_000679"
                     /pseudo
     CDS             complement(12866..14308)
                     /locus_tag="ACHU2L_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(14343..16058)
                     /locus_tag="ACHU2L_000680"
     CDS             complement(14343..16058)
                     /locus_tag="ACHU2L_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR1355665.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            16225..17118
                     /locus_tag="ACHU2L_000681"
     CDS             16225..17118
                     /locus_tag="ACHU2L_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1355666.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17115..17929)
                     /locus_tag="ACHU2L_000682"
                     /pseudo
     CDS             complement(17115..17929)
                     /locus_tag="ACHU2L_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17929..19545)
                     /locus_tag="ACHU2L_000683"
     CDS             complement(17929..19545)
                     /locus_tag="ACHU2L_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1355667.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            19840..20514
                     /locus_tag="ACHU2L_000684"
     CDS             19840..20514
                     /locus_tag="ACHU2L_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1355668.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(20553..22214)
                     /locus_tag="ACHU2L_000685"
     CDS             complement(20553..22214)
                     /locus_tag="ACHU2L_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR1355669.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22410..22838)
                     /gene="ibpB"
                     /locus_tag="ACHU2L_000686"
     CDS             complement(22410..22838)
                     /gene="ibpB"
                     /locus_tag="ACHU2L_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR1355670.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(22950..23363)
                     /gene="ibpA"
                     /locus_tag="ACHU2L_000687"
     CDS             complement(22950..23363)
                     /gene="ibpA"
                     /locus_tag="ACHU2L_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR1355671.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23357..23429)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23668..24000
                     /locus_tag="ACHU2L_000688"
     CDS             23668..24000
                     /locus_tag="ACHU2L_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1355672.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24002..25234)
                     /locus_tag="ACHU2L_000689"
     CDS             complement(24002..25234)
                     /locus_tag="ACHU2L_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR1355673.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            25335..26435
                     /gene="cbrA"
                     /locus_tag="ACHU2L_000690"
     CDS             25335..26435
                     /gene="cbrA"
                     /locus_tag="ACHU2L_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR1355674.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(26396..27733)
                     /locus_tag="ACHU2L_000691"
     CDS             complement(26396..27733)
                     /locus_tag="ACHU2L_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1355675.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27808..28956)
                     /gene="dgoD"
                     /locus_tag="ACHU2L_000692"
                     /pseudo
     CDS             complement(27808..28956)
                     /gene="dgoD"
                     /locus_tag="ACHU2L_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(28953..29570)
                     /gene="dgoA"
                     /locus_tag="ACHU2L_000693"
     CDS             complement(28953..29570)
                     /gene="dgoA"
                     /locus_tag="ACHU2L_000693"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR1355676.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29554..30432)
                     /gene="dgoK"
                     /locus_tag="ACHU2L_000694"
     CDS             complement(29554..30432)
                     /gene="dgoK"
                     /locus_tag="ACHU2L_000694"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR1355677.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30429..31118)
                     /gene="dgoR"
                     /locus_tag="ACHU2L_000695"
     CDS             complement(30429..31118)
                     /gene="dgoR"
                     /locus_tag="ACHU2L_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR1355678.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31396..32052
                     /gene="yidX"
                     /locus_tag="ACHU2L_000696"
     CDS             31396..32052
                     /gene="yidX"
                     /locus_tag="ACHU2L_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR1355679.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32098..32910)
                     /gene="yidA"
                     /locus_tag="ACHU2L_000697"
     CDS             complement(32098..32910)
                     /gene="yidA"
                     /locus_tag="ACHU2L_000697"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR1355680.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33024..33422)
                     /locus_tag="ACHU2L_000698"
     CDS             complement(33024..33422)
                     /locus_tag="ACHU2L_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR1355681.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(33662..36076)
                     /gene="gyrB"
                     /locus_tag="ACHU2L_000699"
     CDS             complement(33662..36076)
                     /gene="gyrB"
                     /locus_tag="ACHU2L_000699"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR1355682.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36105..37178)
                     /gene="recF"
                     /locus_tag="ACHU2L_000700"
     CDS             complement(36105..37178)
                     /gene="recF"
                     /locus_tag="ACHU2L_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR1355683.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37178..38278)
                     /gene="dnaN"
                     /locus_tag="ACHU2L_000701"
     CDS             complement(37178..38278)
                     /gene="dnaN"
                     /locus_tag="ACHU2L_000701"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR1355684.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38283..39686)
                     /gene="dnaA"
                     /locus_tag="ACHU2L_000702"
     CDS             complement(38283..39686)
                     /gene="dnaA"
                     /locus_tag="ACHU2L_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR1355685.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            39983..40063
                     /locus_tag="ACHU2L_000703"
     CDS             39983..40063
                     /locus_tag="ACHU2L_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355686.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40293..40433
                     /gene="rpmH"
                     /locus_tag="ACHU2L_000704"
     CDS             40293..40433
                     /gene="rpmH"
                     /locus_tag="ACHU2L_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR1355687.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40450..40809
                     /gene="rnpA"
                     /locus_tag="ACHU2L_000705"
     CDS             40450..40809
                     /gene="rnpA"
                     /locus_tag="ACHU2L_000705"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR1355688.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40773..41030
                     /gene="yidD"
                     /locus_tag="ACHU2L_000706"
     CDS             40773..41030
                     /gene="yidD"
                     /locus_tag="ACHU2L_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR1355689.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41033..42679
                     /gene="yidC"
                     /locus_tag="ACHU2L_000707"
     CDS             41033..42679
                     /gene="yidC"
                     /locus_tag="ACHU2L_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR1355690.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42785..44149
                     /gene="mnmE"
                     /locus_tag="ACHU2L_000708"
     CDS             42785..44149
                     /gene="mnmE"
                     /locus_tag="ACHU2L_000708"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR1355691.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10746 a        12026 c        11344 g        10228 t
ORIGIN      
        1 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
       61 atgtctctat tctcgacctg atcaatgccg gagaaatttc cggcccaatt gtgattaaag
      121 gacgttacat tgccggtgtt ggcgcagaat acgctgatac tccggctttg cagcggattg
      181 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
      241 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
      301 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
      361 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtgcccgctc
      421 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
      481 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attaacgggc
      541 agaatgcgct gctcgataaa ctcgatgcat ttcgctacct gacgctggac ggtcactgtc
      601 cgggtttggg tggcaaagaa cttaacgcct atattgctgc gggtattgaa aactgccacg
      661 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
      721 gcgaagggtc cgctgccagc aatctcaacg cactggcacc gttgatcaac gaatttaaca
      781 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaagggc
      841 atatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
      901 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
      961 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
     1021 aggtactggt gaaaggcgaa ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
     1081 gactggcgct gtctgccccg ccatatggca ataccattgc ccgccagcca gtttccgcca
     1141 gcgactttgc cctgcaattc acgcccggaa aacgctatcg ggtcattgac gtcatccata
     1201 acgaattgat tacgcactcc cgctccagcg tctacagcga aaatggtttt gatcgcgatg
     1261 atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcctgtggtt
     1321 tgctcggcgg ctttggcctg aatgaaggcg cgctggctgc gacagtcagc catgacagcc
     1381 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
     1441 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca agtacaaagt catctgccgt
     1501 tacccattgc cgggctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg
     1561 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
     1621 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
     1681 gcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaaag cccggcgtca
     1741 tgccgggcaa aagtcaccag ttacgtttat gccactgtca actgctgaat ttttttctcg
     1801 cggcggattt tgcgttcttc cattaccgcc actatcgcca tcaggcagat acaaccaatc
     1861 gcggcgatat ccagcgcggc gaaggtgcct gcccagccgg taaggccgaa taccggcgtc
     1921 ccatcggcaa tcattcccag acctaacttg gcaaagctgt caccaatcag gtaagcaaag
     1981 gtgcctttaa taccatcggc agcgccaatc gcttttttag gtacaaagcc aacagcagcc
     2041 acaccaatca acaattgcgg gccaaagacc aggaaaccca acgcaaagag agaagccaga
     2101 tagatatatt cgttactggc atgttgatac acaccgagcg tggcgataat cagcgccagc
     2161 gcgatgcagg ccaccaggcc acggcgaccg ttcgccaggt cagagagcca gccccacagc
     2221 agcgtaccga ccagcgcacc agcttcaaac agcgtaaagc cctgaatcgc caccgcttta
     2281 gagagtttca gttcctggaa cgcgtatacg gttgaccact ggtcgatacc aatacgtacc
     2341 acatagagga aaatgttggc gaagcacagc agccagatca ctttgttttt cagcacatac
     2401 tcaacaaaga tctgccactt ggtcatatcg gtagattctg tctctttgtc ctcttcgctg
     2461 atctcctcgc cgaacagttc ttcagctttg ccgaggccat aagattccgg ggagtcgctg
     2521 ccgtaacgca ggccgataaa accgacaatc agcgcgataa tcgacgggaa gataaacatg
     2581 ccgatgacat ggccatcgaa caggtaattt gccccgaaca gcgccacacc tgctgcgcct
     2641 gcaccgccaa ggttgtgaga aatattccag aaaccgagga atgtcccgcg tttacgacgc
     2701 ggcgtccatt tggtgatggt ggagtaactg cacgaaccgc cggtactctg gaaaaagccg
     2761 cttaaggcgt agaaggcaat catcaggaac aggctaaccg agccgctgcc catactggca
     2821 ctgaagccca gcatacaaat ggcagagagg ataagcatga acggcaggaa ttgtttggtg
     2881 tttttgccgt cggcgtagta tgaaaccaac gttttaccca cgccataagt gatggagaaa
     2941 cccaggccga tcatccccag ctgcgtcatg ctcaacccgt aggtcgaaat catatcgttc
     3001 tgcgcgatgt taaagttctt gcgaatcagg tacatcgtca ggtagccgat aaagaccacc
     3061 aggtaggatt gcatgaacgg tttgaaccac attttgcgcc gcacttcgag cggaaggtcc
     3121 agggtcggct tgcgaacttg gtttaggaaa gccagcatgg gttactcctg aaatgaatac
     3181 ctgcgtgagg catgcattgt aaaaatcagc agcaaatcag gcctgagata gcgtccaaga
     3241 aaaacctgga aaatttctta gttttgccgg atatgaagtg aaaaggtgag atgcatcacg
     3301 cttcacgcgg tgtctgggcg ttcaaaaagg gcagtaacag cagtgcggaa atcccggcgg
     3361 cgatagagat aaccacaaaa aatccgctcc agtgccaggt atcgagcact ttcgccagcg
     3421 gccaaccagc aagcgacgcc cccagataag caaacaagcc gacaaacccc gtcgccgccc
     3481 ctgccgcctc tttgtgggaa cactctgccg ccgccatacc aattaacatc tgcgggccaa
     3541 agacaaaaaa accaatggtg aagaagcagg ttgcctgcat cacgtagctg gcaaatggca
     3601 tcagccacag cgagccgact gaaagcaaaa ttccggcggc gaaaatcaaa ttcatcggcc
     3661 cacggttgcc gttaaacaat ttgtccgagc cccaaccggc taccagcgca ccgataaatc
     3721 cgcccagttc aaacatcgtc actgccgtat tcgccgtgac cagatcgacg cccagcgtct
     3781 cggacatata caaattgccc cagtcgttga tcgccgcccg gaccacatag accagcacat
     3841 agcaaaacga aagcagccag atatacggat tcagcaacac atatttggtg aggatctctt
     3901 tacgcgtcaa ccctgcccct tcttgttgtt gagcaatttc cagcgcgtcg tgccgccatt
     3961 caccgaccgc cggtaaacct aacgcctgcg ggcgatcgcg tagccgccag cagagaaaaa
     4021 tccccacgac tatcgccata caaccagcaa tcatcatccc ggcacgccag ccgtaatgca
     4081 gcgcgctcgc tgccatcaca atgggaatga gtgcgccgcc gacgttatgc gccgtgttcc
     4141 ataatgccca ccaaccgccg cgctcggtac gtgaatacca ggccgttaac agacgcgcac
     4201 acaccggtga accccagccc tggaaaaagg cgttcagcac ccagagcacg gcaaacgccc
     4261 atagcgacgt cgagaagcca aacagaatgt tcatgatgcc cgtggcgata agccctatcc
     4321 ccataaaata acgggcattt gagcgatcgc tgacaatgcc ggagacaaac ttcgacacgc
     4381 cgtaagtaat gtaaaacagg gtcgctaaca ggccgatatc gctacggctg agcacgccgt
     4441 tagcaaggat ttctggtacc gcggcgttaa aacttttccg cgtgaagtaa aacagcgcgt
     4501 aacccagcca gatggtcagc agaatatgcc gacgccagta gcgataacgg gcatcaattt
     4561 catgtttatc agtcattaat ggcgcatcgg caggcgcttt cagaaacggc aacatcatcg
     4621 aaccttagac ataacgttga ggtagagaaa cgctgacacg cgtgccgtgc agacaggaaa
     4681 tgtgtaatgt gccgcccagc gccgttacgc gctcgcgcat tccggtgagg ccaaaacctt
     4741 gttgcccgga acccggcggc aaaccgctgc catcgtcttc aatcaccagc atcaaccgtt
     4801 catcctgctg ccagccttgc agcgtaaccg cgctggcatc ggcatgtttc acaatgttgt
     4861 tcagcccttc ctggcagaca cgaaacagcg tcacgcgctg gttttcgctt aatgctgatt
     4921 catcgatacg ccattcgaga tggctgacaa tgccgcgccc ttccagctcc atttcccgca
     4981 tcagtgagcg gatggcctgc tccagggtga gatcatccag ctggcgcgga cgtaaccgtc
     5041 ctaacaaacg gcgcaccgcg tcgtaaacgc ccagcgacag ttgttcgatg agctgcccgc
     5101 tctgcttcac actggcgtta tctgccgcca gccgctgaac aatgcccgcc tgagtacgaa
     5161 tagcagtgat ggtctgaccg atatcgtcgt gcagctcacg cgccacgtcg cggcgcacgc
     5221 tctcttcggt ttctaacaaa cgttcagcca gatgctgatt gcgcgccagt tccttttgta
     5281 gcgactggtt aagttcacgc aaccgctgga tgcctgcgcc cagtaacaac cctgtcagac
     5341 tttgcaccag caacgagagt aataaatcca ccagatgatc gcgccaggtt tgactggcga
     5401 tcagcgcgat ggcgttcatc aacgtcgcaa tcagcgctcc ttgccagcca tagtgccagg
     5461 ccagcgcgat aatcggcagc gccagacaga atggcgtaaa gcgcgacagt tcgtccggca
     5521 atcccaactg gagccagaga ctgataacaa acagcagcaa gtaccagacc agatgccgcc
     5581 cgcgccagtt gatgggctga gaaaccagtg acgggccgag cggcagccag gtgttattgg
     5641 cgagatagtg ccagaacacc agacatatcg gggccagcgt cagaccgcca gttaaagtca
     5701 gcaacagcgc attccacgac tctttgccgt gccaaagcca gggcagcgac tgcaacaacg
     5761 ccgccgccgt taacgccgca ccctgtaaca gcaaggtgcg ccagtcacgc tgatggcgat
     5821 agcgcgagat cagcgctacg ggcagtaacg tcagcaaact accgatcatc aataacggaa
     5881 aatgggttaa accgaccgcc tgcgttagcc agtaaatcag cagccactcc gcacccagca
     5941 atacgggcca gtatccgcgc gggcattgca gcattagccc cagacgcaga ccaaacggaa
     6001 ataacagcac cgccatatca gggcgctcaa ccagatgcag gctgatactc cataggcaaa
     6061 accatgcggc agagaagata aaaaagcagg caataacggt aattaagcgg gagaacaacg
     6121 tcttcatcac cagccatcaa acatgcggcg cgccagctct acgtcgttac tgacgcccag
     6181 tttttccatc agattggcgc gatggacgtg taccgttttc ggtgacaagc ccagttcggc
     6241 ggcaatctct ttcaccgcca ttccttgcgc cagtttttcc gccacctggc gttcacgttt
     6301 ggtcagcggg tcctgacgac cggatgccag tttaatggca atatccggcg tcagataaca
     6361 gccgcccgtg gcaaccgtat gcaccgcagc aatgagttca tccgggctac agcgtttgga
     6421 aagaaagccg cgtgcccccg cgttaagcgc ctgctcaacc agcgcaggac tgtcgtgaac
     6481 ggagagcata atcgtcgcca tacctttcgg cagctggctt agcagctcca gaccggagat
     6541 atcgggcatg gagatatcgc aaatacacac ctgcacaccg cgccccggca gccccgccag
     6601 cgcctcgcgc cccgaaccaa actcggcaac tacctgcaaa tcaggttcca gccccagcag
     6661 ctgcgcaaag ccggagcgga cgatgaggtg atcgtctata agggcaacgg tgatcatggt
     6721 cttgtcctgg cgggtaaaaa aaaacgcgct taccttaacg ataagcgcga tgttgttcaa
     6781 gccttgagcg gttactgaaa aaacaccgcg atcttgttaa acatcgtcgg atcggactga
     6841 ttacgctgca ctttcacgac atcttccagc ttagcgattt ggcttatcat ctgctccaga
     6901 cgctggtcgt cattgaccag tagccagata tggcttttgt cgctgtcctg aatcggcaga
     6961 caaagaatgc cttcaacgtt aaaagcgcgg cgggcaaaaa ggccacaaac gtgggtcatt
     7021 acgcccggat ggttgcgaac ggtgagctcc agaattacgt tgttatgagt tgtgttttgc
     7081 atggcttatt cccccaccat ttcagtattc gccgcacctg gcggcaccat cgggtaaact
     7141 ttttcttcgg catcaatgcg cacatggatc agcgccgggc cagggcgatt gatgatttcc
     7201 tgcaatgcag cctgcggatc ggcttcgtta ttcaaatcac aggtttcgag gccgaatccg
     7261 gcggcaatct gcataaagtt gattttgccc ggataggtgg cggcaaaaac gccttgctcg
     7321 tagaacagac tctgttgctg atgcaccagc cccagcgctt cgttgttcat cagaatgatt
     7381 ttgacatcca gctgattttc actggcggtc gccatctcct gaatattcat catcaggctg
     7441 ccgtcgccgg agaaacacaa cactttgcga tccgggttcg ccagcgccgc gccaatcgcc
     7501 gcaggcaggc caaaacccat cgtgcccagc ccaccggagg tcagccactg gcgtgggcga
     7561 ttgagcggat aagcttgcgc ggtccacatc tgatgctgac ccacatcggt ggtgataatc
     7621 gcattgtcat cgacacaggc ggcaacggcg ttgatcaggc cgtaatggct taatggatcg
     7681 cacgctttcg ggattggaca cgggaattcg cgctgcaaat ccgctaccaa ctggtgccac
     7741 tctgcacgcg gttgcgcttc cacccgcggg atcaattgcg ccagcacgtc atcaacatcc
     7801 gcctgaatcg ccacatgcgg ctgcttgatt ttacccagct ctgcgcgatc gatatcgaca
     7861 tgaataattt tggcattcgg acagaactgc tcggttttgc caatcgcccg gtcatcaaaa
     7921 cgcgcaccga gcacaatcaa caaatccgcc tcctgcaaaa tatagttggt actgcgcacg
     7981 ccgtgcatcc ccagcatacc cagcgacaac ggatgcgctt ttggcagcat gcccagcgcc
     8041 attaaagtca tggtggtagg cagttgcgct ttctccgcca gttcacgcac ccgcgcaggc
     8101 gcattgatca caccaccacc cagataaagc accgggcgtt tggcagcgtt aatcattgta
     8161 gctgcgtcac gaatgctttc ttcgctaaag gcgggtgcaa cggctttttc tgccaccgcg
     8221 ggctgagctt caatctcaaa aaccgccgtt tgtacatcct taggaatgtc tatccacacc
     8281 gggctagggc ggcctgattg cgcaatgcgg aaggcatcgc tcatgacctg tgggagctct
     8341 tcgatatgtc tgaccagata gttgtgtttg gtgatgggga tagagatgcc gtaggtgtcc
     8401 acttcttgga aggcgtcggt gccgatcatc gaggcgggaa cctgaccagt gatgcaaatc
     8461 agcgggatgg agtccagccg cgcatcagca atggcggtca ccaggttagt cgcacccggc
     8521 ccgctacagg ccatacagac cgccggttta ccgtcggtgc gcgccattcc ctgagcgata
     8581 aaccccgcgc cctgttcatg gcgagccaga atatggcgga tttgcgtgct ttggcttaag
     8641 gcgtcgtaaa caggcaggat agaaccgccc ggaatgcccg tcacaatctt aatgccctgc
     8701 tgttccagga aatgaacgat aaattctgcg ccggtaaagc gcttacgcgt cgatgttgtg
     8761 cccgaacttg ccatgctcca gtccttttct tctgggccga ctttccggga ggtgcttaaa
     8821 cgaaaaaccc cgcccggttt gcgccggcgg ggttttggaa tcgtgtgttg ttccagtccc
     8881 tacggcgcat tgccgacgac caccaccaca cgcacgacga ccactgcggc ggatggcgca
     8941 gttggtagta gttttgcgtt gaacatggaa gtagtcattg gggaccttgt tggttttgtg
     9001 tttaacaata tttatacaag cacagcttta caggggagac aatggaaaat ttttcagcaa
     9061 gggaaaattg aggggttgat cacgttttgt actgaattgc agataacaaa aaaccccgcc
     9121 ggagcgaggt ttcgtcagcc gcctgcggct ggtaaccgca aagcacactg tattatgtca
     9181 acactgaaag tatacgtgtt ccgcgcagaa cgcgcaattt cggcacgaat tttgacgtat
     9241 ttagtgcata gttgagtatc gttcacagtt tgcgttttgt ccaaatatta ctgtttattt
     9301 atacagtaag cttctataat atcactgtac gcaatgtgtt atgcgggggc cgcatcgtta
     9361 cccggcgcac taagtcctgg ctgaaacggg tggtgccgtc agcgccttaa ccccgcgtga
     9421 gcacactgtg ttatgtcaac aagcacaacg tttctccttg agataccgcg tgcacaacag
     9481 ctggcaacag gcagcggaaa ggtacgtcag ctggcagtgc tcctgaacca caggagacgc
     9541 gtatgagcct ggtggatatc gccattctta tcctcaaact cattgttgca gcactgcaac
     9601 tgcttgatgc tgttctgaaa tacctgaagt aattcagatt caagtcgcac caaaggggag
     9661 cgggtaaccg ctcccctttt atatttagcg tgcgggttgg agtcggatgc gatgctgacg
     9721 catcttatcc gccctaccat ccctcccggc aacatttatt gccgcttttg tttacatatt
     9781 ctgccgctaa acaattcccc attcctggcg tatatctggc taacattcat caatgtgata
     9841 gattcctctc ccgcatttat gggaatgcgt agtgacttat tctaattatt tttataaaag
     9901 catccgtgat aatgaaaagg caaagaaacg tcaatttgtt attgatgttg gtattactcg
     9961 tggccgtcgg tcagatggcg caaaccattt atattccagc tattgccgat atggcgcgcg
    10021 atctcaacgt ccgtgaaggg gcggtgcaga gcgtaatggg cgcttatctg ctgacttacg
    10081 gtgtctcaca gctgttttat ggcccgatat ccgaccgtgt cggtcgccga ccagtgatcc
    10141 tcgtcggaat gtccattttt atgctggcaa cgctggtcgc ggtcacgacc tccagtttga
    10201 cggtattgat tgccgccagc gcgatgcagg ggatgggcac cggcgttggc ggcgtaatgg
    10261 cgcgtacttt gccgcgtgat ttatatgaac ggacacagtt gcgccatgct aacagcctgt
    10321 taaacatggg gattctcgtc agtccgttgc tcgcaccgct aatcggcggt ctgctggata
    10381 cgatgtggaa ctggcgcgcc tgttatctct ttttgttggt tctttgtgct ggtgtgacct
    10441 tcagtatggc ccgctggatg ccggaaacgc gtccggtcga tgcaccgcgc acgcgcctgc
    10501 ttaccagtta taaaacgctt ttcggtaaca gcggttttaa ctgttatttg ctgatgctga
    10561 ttggcggtct ggccgggatt gctgcctttg aagcctgctc cggcgtgctg atgggcgcgg
    10621 tgttagggct gagcagtatg acggtcagta ttttgtttat tctgccgatt ccggcggcat
    10681 tttttggggc atggtttgcc gggcgtccta ataaacgctt ctcaacgttg atgtggcagt
    10741 cggttatctg ctgcctgctg gctggcttgc tgatgtggat ccccgactgg tttggcgtga
    10801 tgaatgtctg gacgctgctc gttcccgccg cgctgttctt tttcggtgcc gggatgctgt
    10861 ttccgctggc gaccagcggc gcgatggagc cgttcccctt cctggcgggc acggctggtg
    10921 cgctggtcgg cggtctgcaa aacattggtt ccggcgtgct ggcgtcgctc tctgcgatgt
    10981 tgccgcaaac cggtcagggc agcctggggt tgttgatgac cttaatggga ttgttgatcg
    11041 tgctgtgctg gctgccgctg gcgacgcgga tgtcgcatca ggggcagccc gtttaagcgc
    11101 acgtcaccgc agcatcgtca tcagctccat gggagaacga tgctgcttta tcagatcacg
    11161 catcacccgc atatgcggtg cggagtaaga ataaaacgcc tgatagcccg cacaaagcac
    11221 gctgtctggc gtctcctgcc gagcgtgcgg gcacaggcga tgccagtcgc acgcctggca
    11281 gtcacggcgc ggttgcgggt tttcgctgcc acaccatttt tgtaaggtgg cgtcaaaaag
    11341 ctcaatcgag atacgactga tatctgcccg cacccaacac tcaaacaggg caattaaaaa
    11401 tttcccccac agctctgctg tcaccgaagc ggccgtcaga tcgccccggt catcaaattc
    11461 aagataggga atgagctgta cggagcggat gccccgctgt ttgaaattgt gataaaaatc
    11521 ggcaggttgc agagcatcct tgtactctac aaccagctta tgcctgtcct cttcagcatc
    11581 aatgacctga cttcctgtca tgcgactctc tcaataaata cgataagttg atgtatatgc
    11641 gttacagcaa acagtattgc gagagataac accgcgaggg agatcaaaaa tttgtcacgg
    11701 actacgtgaa attgggaaaa atcgctattc gtgacatcca ttaaattgcg attacgggtg
    11761 tagtgccaga ggatcagcgc cacgatggcg aggatgccaa tcgaaatcca gaataacatg
    11821 cccgcctggt gccagttgtg tttgatagcc aacgccatca acgcgccgta gcccagcatg
    11881 gtacgaaacc acgccagtga tgtgcgctcc ggctgcagcc ctgggtcggc aatgcgtctg
    11941 gctttacggc tatccggcat acaacaccag tcccataacg atcaccgcca caaccatcaa
    12001 tattaagctg atgattaata agctgttggt gtagggcagg tcctctttca gacgcatcgc
    12061 tttttcattg cgtaaccagc gcaggtagcc atacattgct aacccgccgg aaaacagaca
    12121 caacaacaac gccagcagtt cacgaatgac gggcgtggcg aaatctggcg caagttgatc
    12181 aagcccaacc cccgccgcca gaaatcctag tgcggtacgg atccacgcca gaaaagtacg
    12241 ctcattagcc agcgagaagc ggtaatcggg cgcttctcca aggcgggaaa ttttcattgt
    12301 tgctcctttg caatttcact aacgctctat ctttagcggt tttttttaag aatttcgtcc
    12361 gcccgtttcc tgatatgctt ttgccatccg ctacaaggag gtcagatggg tatcatagcg
    12421 caaaataaaa tcagttcatt gggcatgctg ttcggtgcca tcgctttaat gatggggatt
    12481 atccactttt cattcggccc tttttccgca ccaccgccaa cgcttgaaag catcgtggcg
    12541 gacaaaacgg ctgaaattaa aagaggtctt ctcgccggaa taaaaggcga aaaaattacc
    12601 actgtggaga aaaaagagga catggatatc gataaaatcc tcgatcaaag cggtatcgca
    12661 ctggccattg ctgcgctact ttgcgcattt attggcggta tgcgtaagga aaatcgctgg
    12721 ggcattcgcg gcgcgctggt ttttggtatc ggcatcgtgt gcagcatttt gctgatattc
    12781 ctgatttttt ccttcctgac cggcggttcg ctggtttaag cagcctgaat aatgcccgcc
    12841 gggtgatcat cagcgggcat tatcgtcaga atttctgttt tttctcctcc accttcactc
    12901 cttgcgtcgg taggccggtg tcatagtcgc gtaccactgg cgaatagcca tcttgtgggc
    12961 gtgggcgaaa cgcccccatc cagcgcggtc gtgcatcttt acgccacgga cgcagactcc
    13021 attggtaact gcggaacgga tcgcgaattt tgtccatgta atccaataag gcgtcatgca
    13081 ttttgctgcg aacgtctgca aaacggatat catcgatcag gttatgcatt tcatttgggt
    13141 cattacggcg atcgtaaagt tcatcactgg tgaagaggtt gagcaccagt ttaaagtcat
    13201 ccgtcaccca gcaacgcacc ggaataaaac cgccaaagct gtcatgctca atctcgtagc
    13261 ggttaaattc caccatcacg ccgcgtggct ctttcacggc aaggatattt tcccccggca
    13321 gaatctctgg tttttcaata tctgccagcg ccatcattgt cggcagtaaa tcaatatgac
    13381 tgactggcgt atcgacctgt cgccgctccc cttgcggcga acggatgatc agcggaatac
    13441 gggtgatgtc gtcatacatc gccgctcctt tactgatcag cttatgtgcg cccatcattt
    13501 caccgtgatc ggaggtataa atgacccacg tattttcacg ttgctctggc gttaaggcat
    13561 tgatgacacg tccgatttgg tcatcaacaa agtcattgca ggcaaaatag agcggatggt
    13621 gataaagccc gtcatcaccg actggcgatg gcatcgcctg cgcccataag cggtgatgtt
    13681 ccggtttgtt cgccaggtca tcctgtgatt tctcgccaag ttcgtagtaa aaatcagcgt
    13741 atttctctaa atactccacc ggacaggtga acgggtgatg cggctcatca tacgaaacca
    13801 ccatcaggaa gggttcctcg gcgcgcacgg gctattgcag aaaatctacc gctcgattgc
    13861 tgatgcggtg cgcccaggtg aaggtttcgt caatatggtt cgcctgtaaa tcttcgacgc
    13921 tgtttaggcc attgcgccac aggctgatct ctttttccgt cagttcgcta agatagttcg
    13981 ccccatcaaa ccagtaatca gcatcccact ccggcggaca ctcgccagtg ccgaaatagt
    14041 catgaccgtc gagatgccat ttgccgatgt aacaggtgtg atagccggca tccttaaagt
    14101 agcgccccat agtggagatg tttttgcctg gcgcgacgtt gttggtccac gggccggact
    14161 ggttagcgta gataccggtg aatagtccgg cccgtgcagg cgtacaaacc ggtgaacaag
    14221 tgtaggcgga attaaagcga ataccttccg ccgccagact atcaatattt tgcgtattca
    14281 gcggtttacc gctatagcaa ccgaccatat tggtggcctg ggtatcggtc aattggggcg
    14341 tttcataggc tatccttcgc gtcgggagta aaggttacgg ctgggtcgtg acgatgccac
    14401 cagctgtcaa aaatcaggta gacaatcact acggcagcga tgaaataact gatcatcgct
    14461 aaccagcgcg taccgtagcc gccgaattca gccagcccga catagacgcc aatcatcgcg
    14521 aacaggatac caattgacgc gatcttgacg tttttccacg gtttcatgtc caccgcaaac
    14581 gcatctttga aggtgaacgg cgtggcgcgc ggtttgataa aaccgatcac cagcatcacg
    14641 accacgttga tgcagaacgt acaggccagc acatagagga aatggaagtc gaacttcacc
    14701 agatagttga tggtgatgta gctgattatg ccaatcccca tcgccacttt tgccgccagc
    14761 gccgggatgc gcggaaagaa aaagcccata atgatgatgg taaccagcgg cacgttgtaa
    14821 atgccgttga gctgtttcat ccagctatac agcccctgcg gcgcgttcgc gatccacggc
    14881 gctaccaaaa ccgaaacgat agcgataaag aaaccaaatt tgcgcccgac ggtgaccagc
    14941 tgctgcggct cggcattctg gttaatgatg cgacggtaaa tacccatact gaataatgta
    15001 ctggcgctat tcagaaagcc gttgaaggta ctgatcaccg caccaaataa caccgcgccg
    15061 aagaaaccct ccagtggcac tggcagaacg ttattgacca gcgtcgggta ggccatgtcg
    15121 gctttcggta gatcctgata cagatgaaac gcaatcaacc ctggcagcac cagtaccagc
    15181 gggtcgagca ttttcagcac cgccgttaac agcgccccct tttgcccttc cgccaggctt
    15241 tttgacgcca gcgtgcgttg cacgatgccc tgatttgtac accagtaaaa ggtgttcacc
    15301 agaattaaac cggtaaatgc cgcgccaatc ggcaagggat cggtcgggcc accgactgag
    15361 tttaatttct cggcgtgaac ggtggtaatt tgctcaatgc cctgcataaa gctgcccttg
    15421 cctatcgcga tcaggccaaa taccggcacc atcaacccgc cgataaccag cccaataccg
    15481 ttgatggagt ccgccactgc cattgcgcgc agtccgccga tcaccgcata caaaattccc
    15541 gccagaccaa gcaaaattac cagtagccag atagccgcac cgtgagaaat ctgtagcgat
    15601 tccccgacgt gaaacaggct gttgagcgcc aacgcgccgg agtagagaac aatcggcaga
    15661 aagcagacgc cggtggcaat gaggaagcag aagtcgatga taatacgcgt cgttttatca
    15721 taacgttcct ccagaaaatc ggggatggtg gcaatgccgc gcttcagata gcgcggtaaa
    15781 aagattagcg cgaggaagat cagcgtcacc gctgaagtca cttcccagcc catcaccgac
    15841 atgccgcttt tgtaggcctg cccggaaaga ccaaccagtt gttccgtgga aaggttggtt
    15901 aacattaacg aagcggcaat aaccggcgct tttagtgaac gtccggcaag aaaatagcct
    15961 tgttgcgatc cggtatctgt tttgcgaacc ttccaccagg tgatcaccgc caccagcagc
    16021 gtaaaaccga caaaactcaa gatttgtaac gaattcatct catagccctt atttatgttt
    16081 atgtcccgga cgcatgaata tagggagagg taaaatgcgt cgggtcaggc agtcagattc
    16141 ttaaaataca aacgtcgtat ccctgaacgg attgttttct gttaagttca ggttgtgagc
    16201 ataattcgcc ggagggatat taaaatgaat ggaaaattgc aaagttcaga tgtaaaaaac
    16261 gaaactccgt acaatattcc cttattgatt aacgaaaatg tgatctccag cggaatttct
    16321 ctgatctcgc tctggcatac ctacgccgac gagcattacc gggtgatctg gccgcgggac
    16381 aagaagaaac cgcttatcgc caactcatgg gttgcggtgt ataccgtaca aggatgtggg
    16441 aaaattcttt taaagaatgg cgaacaaata actctgcata gcaactgtat tatattttta
    16501 aagccaatgg atattcactc ttatcactgt gaaggtttag tctgggaaca gtactggatg
    16561 gaatttaccc ccaccagtat gatggatatt tccgttggtc agcaaagcgt tatttataat
    16621 ggcgaaattt ataatcagga actcaccgaa gttgctgagt taataacttc accagaagca
    16681 ataaaaaata atctggcagt cgcttttctg acgaaaatta tttatcagtg gatttgtctt
    16741 atgtccgcag atggtaaaaa agatccacaa cggcggcaaa ttgaaaaatt aattgccact
    16801 ttacatgcca gtctgcaaca acgctggagc gtagctgata tggctgccac gatcccctgt
    16861 agcgaagcct ggttgcgtcg tctgttttta cgctataccg gcaagacgcc gaaagaatat
    16921 tacctcgatg cgcgtctgga tctggcgcta tcgctattaa agcaacaagg aaactcggtt
    16981 ggcgaagtcg ctgatacgct caacttcttc gactcctttc atttcagcaa agcctttaaa
    17041 cataaatttg gttatgcgcc gtcagccgtg ctaaagaata cggaccagca cccaacggat
    17101 gccagtccac acaattaacg taactctggc cagtacgctt tgttggcctc cagcaactca
    17161 tccagaatag ctttggcgac cgaggcgctc ggtaccgttt tcgacaacgt aatcgcctgc
    17221 cacaggtgct gatatgaacg ctgttcccag gcgtccacga ccagtttttc caccgccact
    17281 tgctgactca ttaacccttt ctgaaactgc gggatatcgc cgaccaccag tggttcaggc
    17341 cgtagtagcg cacgcgcatc tgtttgcgat cttgcagccc aacaatttgc gccatccggc
    17401 tttcaatacc gattggcatg tcacagatgt tgaggatttt cgcattcggg cgcagacgac
    17461 gcgtggcttc tgcgacaatt gctgccgggt tggagtagtt gagcatctag gcgtttggtg
    17521 aatatttttc catataatcc accagttcca ggacgccgcc aatggaacgc atgccgtacg
    17581 ctattccgcc gggtccgcaa gtttcctgac caacaacacc gtggcgcagt gggatttttt
    17641 catccagttc gcgcatcggg tacttgccga cgcggatgtg cgccatgaca aaatcaacgt
    17701 cgctgaacgc cacttcagga tcggtggtgt aactaaacgc aatgtccggc gctttttctt
    17761 taaggatgac tttacaggct tcggcaatca cttcctgccg cgcgccatcg ttatcataaa
    17821 atttcagtgc acgaagcggg aaacggtcct gattcgccag gagcatcaac acgatgcctg
    17881 gcgtaaaggt gcttccaccg cctgcgacaa ccactgagaa tttggtcatt atactgcctc
    17941 cgtaatggca acattttctg ctgattgatg agaattaatt aagctatcga gctgttcacg
    18001 caattgggat acatgcagac caatgatcac ctgaatggca tcgccactac ggaagacgcc
    18061 gtgcgctccc agctttttaa agacttcgtc atccagcgtt tgtgacatgt catgcagtgc
    18121 aatacgtaga cgcgtcgcgc aattgttaat gctggagata ttgccgaccc cgcccagggc
    18181 ttgcaggata ccggcagcct gatccagctc tttttttggc tctgcagcgg tggtttggcc
    18241 tcgcgaggct ttgtattcgg cttttgagta gagtttcact tccgcatctt cacgtcccgg
    18301 cgtgcacatg ttgaactgca gaatcagggt gcggaaaacc acgaagtaca gcagggtaaa
    18361 gcacaaccca atggcgattt gggtcagcat catatccgcg tggttgctga acatcgggat
    18421 ccagttttgc ggtaaaacct ggtcaatcag acctccgccc atgttgccca ccacaccaaa
    18481 gagatacatt acggtcgaca ttgaggccgc cagcacggcg tgtaccgcaa acagcaacgg
    18541 tgaaatgaac aggaaggtaa attccagcgg ttcggtaatt cccaccagca tggcggttaa
    18601 ggttgcggga atcagcaagc ccgccacttt tacccgattt tccggtgcgg cagtgaagta
    18661 catcgctaaa gaaatgccca cggcaccaaa gatttttgag ttaccgtgca gggcaaaacc
    18721 gccttccggg aacaacgatt tcagtggctc ggcgctcaga ctgaactctt gcagatgctg
    18781 cgcccagtac atctgaatgc cgccttcaac agctgccgga ccaaagataa acggtccgta
    18841 gatgaagtgg tgtaaaccgg ttgggatcag aatacgttcg aggaaggtgt aaacccacac
    18901 cccaagcgca cccgccgaac gcaggaacgc ttgcagagat tcaatcccca tttgtacttt
    18961 tggccagccg agcaatgtca gccaggcaca ggggatcatc accaggaagg cgataatcac
    19021 cacataagac gtcccctgga aaatgccgag aaaaaccggc agttttttat cgaacagacg
    19081 gttatgcagc gccgtcacaa tgccggaaat gataattgcg ccgataatgc tggtatcgag
    19141 ggttttaatc ccggccatca ttgtcagacc gctacctgcc accgcgtcct gagtgaaatc
    19201 gacgccgaag tagcttcccc aggtcattcc catcgcattg atgaaatagt tccaggtcag
    19261 gaaactcacc atcaccgcca gacaagcacg tccctgcgct tgcttagcaa ggccaatggg
    19321 taaaccgaca gcaaaaatca gcggcatatt acggaatacc gtccaaccgc cctcttcaat
    19381 aatgtgtacg atttgcgcga ataaactgtt cggatcggtc agtgattccc cgacaaacat
    19441 cgggttttgc agcaagatgg caagacccac cacaatcccg gcgaagggaa acagcagcac
    19501 tggcgtgaac atcgcgccgc caaagcgttg aatttgactg agcatttcca catcctcttt
    19561 tctcaattct gtaagtagga agcctgattg gttatgcggc agacctggag cgaggatagg
    19621 aagatgcgat cggcagaaca tgacgaccca atgggattcg tgatcgcttt catgcttttc
    19681 gttgggcggt agagatctac gttttcgcga aaaagtagac atctgtgagc acaggtacac
    19741 agcatcgaac acatctttaa aaaaaagatt tttttcaatc gattaagcag aacttgtggg
    19801 cgcattaccc gtgcttgcag gcaaaaaaga gagctagaga tgatctacaa aagcattgcg
    19861 gagcggttaa gaattcgact taattccgcg gatttcacgc taaacagcct tcttcccggt
    19921 gaaaaaaagc tggcggaaga gtttgcggta tcgcggatga ctatccgtaa agccattgac
    19981 ctgctggtag cgtgggggct ggtggtccgc cgccacggca gcggcactta cctggtgcgc
    20041 aaagatgtgc tgcatcaaac cgccagcctg accggactgg tggaggtgtt aaaacggcag
    20101 ggaaaaacgg tcaccagcca ggtgctgatt tttgaaatca tgcctgcgcc tccggccatt
    20161 gccagccagt tacggattca aatcaacgag cagatctact tctcccgtcg cgttcgtttt
    20221 gtggaaggga aaccgctaat gctggaagac agctatatgc cggtaaaact gttccgtaac
    20281 ctttcgctgc aacatctgga agggtcgaag tttgaatata ttgaacaaga gtgcgggatt
    20341 ttgattggcg gtaattatga aagcctgatg ccggtgctcg ccgatagact gctggcgcgg
    20401 caaatgaagg tagcggaaca cacgccactg ctgcggatca cctcgttgtc atatagcgag
    20461 agcggggagt ttttgaatta ttcagtgatg ttcagaaatg ccagcgaata ctaggtggag
    20521 taccatttac ggcgactcca cccggaaaaa agttaaccga tactccagaa gagcaccgcc
    20581 agtaattggg gggtgataat gcgcaggaac atcactaacg gatagacagt ggcgtaagag
    20641 agcgccgccg caccactggt tggatgaaga ttattagcaa acgccagcgc cggaggatcg
    20701 gtcatggaac ctgccagcat cccgcacatg gtcaggtaat tcattttggc taacatccgc
    20761 gccagaatgc caacagtaat cagcggaacg gcggtgatca gggcaccata accaatccag
    20821 cttagccctt cgccattgac cagggtattc acaaaatccc caccagattt cagaccaacg
    20881 accgagagga acagcacgat ccccagctcc cgcagcgcga ggttggcact tggcggcata
    20941 aaccagtaca gcttgccgat actgccgata cgcccgagga tcaacgccat aatcagcgga
    21001 ccgcccgcca gccccagttt caacgcggcc gggaatcctg gcacaaagac gggaatagaa
    21061 cctaacaata cgccaagccc gatgccaata aacactggca gcatctgaac ctgttgcagt
    21121 ttttgctgcg cattccccag cacattggca acggcatcaa ttgcggacgg acgccccacc
    21181 aggttgagga tatcgccgaa ctgcaggctg atatcgccac tggcgaccag ttcgaccccg
    21241 gcacggttca ggcgcgagat aacaacgtca tagcgttctt taaagtgcag gtcgcgaata
    21301 cgttttccga gcacgttttc attggtgacc accacacgct cgacgcgcaa atcagtgcct
    21361 ttcgtagaca gcgaggtatc gacctcctga ccaatcacca gttgcgcatt atgtaaatcc
    21421 gctggctgcc ccaccagatg cagcaaatcg cccagttgga taatggtatc tggcgaaggt
    21481 acttttaggg tttcttctcg tttcagacgc gagcagataa ttttgtcgcc gttgagaatc
    21541 ggtacatctt taatggctaa atcatgcagg ttagggttct caacgcgaat attgatagtc
    21601 ttgatcagcg cgccgccatt ggtgcgtgaa gactcgtgct gctgagcttc tgtctcgaca
    21661 ttgacgcgga aaataacccg caacatccac atggtgaaca aaatcccgca aatgccgaat
    21721 ggatacgcca tcgcgtaact catccccatc tgatcgacca tttccattgg tgtacccagg
    21781 tcgcgcaaga tctgctgccc tgcccccagc gctggcgtat tggttaccgc accggagaaa
    21841 atccccagca ctaccggcag tggaatatca aacagtttat gcaggatggc ggtaaccaga
    21901 ccaccgatga tgacgatcag aacagcaaac aggttgaggc gtaatccgga gacgcgcaat
    21961 gaggcaaaga agcccggccc tacctgaatc ccgatggtat aaacgaacag gatcaggcca
    22021 aattcctgaa taacatgcag catatcgcta cttaatgtca tccccgcctg agaaacaaaa
    22081 tggccgacga tgatcccacc aaacagcacg ccgccaatac ctaatcctat gccgcgaaat
    22141 ttgacgttgc cgataaacaa accgacgact gccaccaaag ccagaatact gaccgttaat
    22201 gctatatcac tcatcgtcca tttctcttga gataacattt taattgctac ggattatggc
    22261 tgaatcggtg aagcagattt gccttatagc gcacattatc acgggaaaga acgtgccgaa
    22321 atatcttaaa cagtcgaaat ttaacgcatt gttgaagaag aaagacaaaa aaagccccac
    22381 cagaatggcg gggcaaagag aatagctagt tagctattta acgcgggacg ttcgctgata
    22441 gcgatacgct gcgctgcgat gggttcaggc tcattacgaa ttaaatcaat atgcagtaaa
    22501 ccgtttacga aggttgcgcc agagacttcc atattttcag ccagcgtaaa actcaggcta
    22561 aatggctgat tcataagccc ttgatgcagc cattttttct cttcttttgg ctgctccggc
    22621 gtgcctttta cgctcaggcg cgtgccttcc agttgaatct ctaaatcttc ctgacggaaa
    22681 cctgccagcg caagggtaat gcggtagtgg ttatcgtcgc ttttctcaat gttgtacggc
    22741 gggaagctct ggctttcacc ggcgttttgc agtgcgttgg ccagtttgtc aaaaccgatc
    22801 cattgacgca tcagtgggga taaatcgaag ttacgcatag tcatttctcc ttctaagaag
    22861 cgagtaagta cctgcaaatc cgaagattcg catatgctcc ctgacggcga gcatggagat
    22921 gtcaggccgc gccaggcggc cttagggaat tagttgattt cgatacggcg cggttttttc
    22981 gcttccggaa tcacgcgttc gagatcgata tacagcaaac catttaccag gttagcccca
    23041 cgaacatgaa tgttctcagc taactggaat ttgcgttcaa agttgcgttc agcgatgccc
    23101 tggtacagat aggtgcgctc tttttgttcg tcggcgtgag cacctttcac caccagcaga
    23161 ttatcctggg cggtaatttc cagttcgctc tcagcaaaac cagccacagc gatagcaatg
    23221 cggtaatggt tttcgtctac cagttcaacg ttatacggag ggtagccgcc attactctgg
    23281 ctctggttgt tttctaagtg gttaaacaaa cggtcaaatc caatagcaga acggtaaagc
    23341 ggggataaat caaagttacg cataatcaat agctcctgaa atcagcgaga atgtaagacc
    23401 ttccacaatg gacaggtcag gtagccagaa cacccatcag gcgcgtcctc atcgactacg
    23461 atgtaaaatg ggtctggaaa tgaacttttc aagccttatc agcggacttt tttttgcagt
    23521 ttatggtcta ttgcatagac tgagggggca gcacgaaacg ttaaagtgcg atagccgcca
    23581 cagtgcgacg taatgatgat gttattttca gcaagggatc gctataactc atcaggccaa
    23641 cacccacgat caacagatga atttattatg ataagaaatg tgttgttagc gttcatgata
    23701 tgcagcggaa tgacattact cggaggatgc tccagcgtga tgtcccatac cggggggaaa
    23761 gaaggaacgt atcccggcac ccgcgccagc gccaccatga ttggcgacga tgaaaccaac
    23821 tggggcacta agtcgctggc gattctcgat atgccgttta ccgctgtaat ggacacgctt
    23881 ttactgccgt gggatgtgtt ccgcaaagac agctcggtgc gctcgcgcgt tgaaaaaagc
    23941 gaagccaacg cgcaagccac caatgcggtt attccaccgg ccagaatgcc tgataactga
    24001 tttagcgatc agtttcggtg atccacagct ggccaccttc catccacgcc agccattgac
    24061 catccggcga gaagaccacg gcgtccgcag aaggtggatt ggcgtggttt tcggttaaat
    24121 actcaacctc gccgctttgc gcatgggcac aaacaattcg attatccagc acaaagccca
    24181 accattctcc tgacggatgc cagttaaatg cagactgaat atccgttttg ttatgggtta
    24241 acttgcgcgg ttcgccgccc tgtggcgaga taagccacag ttgcacaatg ccgttatcat
    24301 cacgcattaa aaacgcgatt tgcgtaccct gcggattaca gcgcacccag tggcgcggga
    24361 cgttgactaa ccccggataa gcccgatgat gggtaaaggt taaacgtcgc tgcacgacgc
    24421 cacgcggtgg cgcgggtagg gttgtttccg ttccacttaa cggcgcatca cctgccgatt
    24481 tccagctagc ttcatcttgc ggtaactcaa cgataaatag ctccggcact ttctcgccct
    24541 ttggcgaaag tgtgtcgcca ataaacgcca gcgcgtgatt tcctacccat ccttcttcat
    24601 aagcacgatt gatttcatca ctgccaggct gcggcgtggg cgtggtttta ctcaccagca
    24661 cacaccagtg gctaccgctg tattcacgcg gatgttgctt ttgtacgttg accgggccaa
    24721 acggtgcagc aaccccgacg tttcgcaaat ccagcgccgg atcgagttga tgcattacat
    24781 ggtcgttata ggtaaagctc cccctttcac cgttcgggct aaagacatgc acatggctgc
    24841 caccgcgcag cgctcctggc gtatacggcg cggtaatatc cattgcatcg agattgctca
    24901 ccttgccgcc ttcagcaatg actccgcgtc gatgatggaa gtcgtaatgc catgtttcat
    24961 caggattttc agggccgtga ataaagacat atttctctga cttcggatga acggtcacca
    25021 cgccgacgtg tgcgccctgt gacgcgcgat agataacctc gacctcgcct gtatggatat
    25081 tcacacgctc aatggtctcg ccggtaaacg acgccccaga aggacgcacg tcaaatacca
    25141 gccactggct gtcgggcgtc caggtattgg tatttgtatt ggtattggta ttggtgagca
    25201 ggtgattacg gggagcaaag gttatctgtt tcatggcgcg gtcctgatac aaaacggttc
    25261 ccgccatcat acttcacaag gacttcactt tcagacaata gtcttttgcc atcgtgatga
    25321 atgtgagtga aaagatggaa catttcgacg tggcgattat tggcctcggc ccggcagggt
    25381 cggcgttggc acgaaagtta gccggcaaaa tgcaggtgat cgcgctggat aaaaagcacc
    25441 agtgcggtac tgaaggtttc agcaaacctt gcggcggtct gctggcaccg gacgcgcagc
    25501 gatcttttat tcgcgatgga ctgacgcttc ctgtcgatgt gatcgccaat ccacagattt
    25561 tcagcgtcaa aactgtcgac gtcgccgcat cgctcacgcg taactaccag cgaagctata
    25621 tcaatattaa tcgccatgct ttcgacttgt ggatgaaatc gctgatcccc gccagcgttg
    25681 aggtttatca cgatagcctg tgccgaaaaa tctggcgtga ggatgataaa tggcatgtca
    25741 tttttcgtgc agacggctgg gagcaacata ttaccgcccg ctatctggtc ggtgccgatg
    25801 gcgcaaactc gatggtgcgg cgacatctct acccggatca tcaaatccgt aaatatgtcg
    25861 ctatccagca gtggttcgca gagaaacatc cggtgccgtt ctactcctgc atctttgata
    25921 atgcgataac tgactgttac tcatggagta tcagcaaaga tggttatttt atctttggcg
    25981 gtgcctatcc aatgaaagac ggtcagacgc gtttcacgac gctgaaagag aaaatgagcg
    26041 cctttcagtt ccagtttggc aaagcggtga aaagtgagaa atgcacggtg ttgtttccct
    26101 cacgctggca ggattttgtc tgtggtaagg acaacgcctt tctgattggt gaagcggcgg
    26161 gatttatcag cgccagctcg ctggagggga ttagctatgc gctggatagc gcagagattc
    26221 tgcgtgcggt gttcctgaaa cagccggaga agagcaacgc cgcttactgg cgcgccacac
    26281 acaaactgcg tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg accgcaccgg
    26341 ctttaagaaa gtggattatg cgcagtggtg tggcgcatat tccacagttg aaagattatc
    26401 caacgcgctt cacatcaccc accagcagga tgtaagagag cgcgccaatc aacgcgacgg
    26461 cggagatata aaccagtgca ggcgcgaaac cgtaaccctg cgccaggtac cccaccacca
    26521 gcggaacggt gatgccgccc agaccaccgg cgaagttaaa cacgccgccg gttaaaccaa
    26581 tcaggcgcat cggtgccaga gaagagacca gcgaccaggt aatcgaagca aaaccgttgc
    26641 cgaagaatgc cagcgccatc aggcacataa tcatcatcgg atcgttggtg tagttagcgc
    26701 ccataatgca ggtggagatc agcaagccgc agataatcgg cgttttacgc gcaaagccca
    26761 gtgaaaagcc cttacgtacc agcagatccg cgacccagcc agagagcagg acgccgacaa
    26821 acgccgcgag gaatggcacc gtggtcataa agcctgcttt cagcgccgtg attccttttt
    26881 cctgggttaa atagttcggg aaccaggtta agaaaaacca cagtgcagaa gccaccgcaa
    26941 attgcccaag ataaacgccg atcagtttac gatggaacac cagtttccag tctttggctg
    27001 ttaacggctg acgcgcctct ttcttcaccg gcgcatcacc atccaccaga ccgccgccat
    27061 cacgaatgta atccagttca gctttgctga tacctttggt caggcgcggc ggctgataaa
    27121 ccttaaacca aatcagcgac cagataatgc cgataccacc agtgacaatg aacacccagt
    27181 gccagctcaa catctcctga atccagatca gcagcggagt caaaaacgcc agaccgacaa
    27241 actgaccaga cgtataaaaa ccaacggcgg aagcgcgttc atgttccggg aaccagctgg
    27301 tcaccatccg gttattggtc gggaaagcgg gcgcttcgaa aataccggtt atcgcacgca
    27361 gaccaattaa tgacattaat cccgtggcaa agccttggaa taaagtcgcc actgaccaac
    27421 caaatatcgc aataaaataa gtcacgcgag aacctacgcg atctaaaaac caaccgccgg
    27481 ggatctggca tagcgtataa agccaggcga aggccgaaaa tacatagccc atttccgctt
    27541 tcgtaatgcc gaactcttcc tgaatatggg cggaagccac ggccaggtta gcgcggtcaa
    27601 cataacaaat gaccaccgta ataaagatca tcaccagcgt cagataacgc cgacgccccg
    27661 gctttgctgc attaacggga atatccatag cgagctttct ccagattttg ggcatagcga
    27721 agccgctcac catgccctgt aatttacaga gggttatatt tttgtattgc tgttttagtg
    27781 cccgatgagg ggcttacgtg gcaggaatta ccactctgct acgctgttat cttcatgacg
    27841 ccagagcgga ttacgccagt ccggggcatt tttactgaac tcaataactt tagcttcgtc
    27901 gatttccaca cctaagcccg gtttcgttaa cggtttaaag aagccgccaa ccatgctgaa
    27961 gtcttctttg tttttcacaa agtcgagtaa ctccgcgcct ttgttgtaat gaatgcccat
    28021 actttgttac tgaagtaccg cgttatagga aacgaagtcg atatgcaggc aagccgccag
    28081 tgcaatcggt ccgaacggac agtgcggcgc aagggtcaca tcataggctt ctgccattcc
    28141 agcaattttg tagcattcgg taataccgcc tgcatgggag agatccggtt gcagaatcga
    28201 aataccgcct gcctccagca cgcgtttaaa atcgaaacgt gagaacatgc gctcacccgc
    28261 cgccagtgga atatgcgttt gtgccgccaa tttcgggtag tattcggctt gttccgccag
    28321 caccggctct tcaataaaca gcgggcgata cggctccagt tctttaatca gcactttcgc
    28381 cattggcgcg ctgacgcgac cgtggaaatc aagaccaaac tcaatctgat tgccaaaagc
    28441 ttcacgaatt tgtgccacgg tgttgactgc cgcatctacc gcgcgggagt tatcaattag
    28501 ccccagttct tcacaaccgt tcagtttgaa ggtatcgaag ccgatttcgc gcagggtttt
    28561 aatgccgtcg ataacatccg ccggacgatc gccgccgacc caactgtagg ctttaatttt
    28621 gtcgcgaacc aggccgccca tcagttgcca gactggcgca ttcagcactt tgcctttgat
    28681 atcccataac gcctggtcga tcccggcgat ggcgctcatc aggattggac cgccacgata
    28741 aaatccggcg cgatacatca cttgccataa gtcgttgatg cgcgaaggat cctgaccaat
    28801 caaatagtca cccagctcgt gaactgccgc ttccaccgta cgggcgcggc cttcaatcac
    28861 gggctcgccc caaccgacca cgccttcatc ggtttcaatt ttcaggaaca tccagcgggg
    28921 aggtaaacga tacgtggtaa ttttggtgat tttcattgca ctgcctctcg atacgcctta
    28981 acaaatgctg ctgcctgctg cgcggtgcgt tctacggact gcccggcgcg ataaagatcg
    29041 ctgcctaagc ccgccccaac acaacctgcg tctatccact gcgccaggtt ttctggcgtc
    29101 acgccgccaa cggcaaagac tgcgatgtcc ggtggtaata ccgcttttaa cgctttgatg
    29161 tattgcggac caaaagccga tgacggaaat attttcagcg cctgcgcgcc cgcttcgagc
    29221 gcggtaaagg cttcggtcgc cgtcgcgcag ccggggcaaa cggtcatgcc gtagcccacc
    29281 gcgcggcgga tcacttcact atggatattg ggcgtaacga tgagctgaca gcccatcctg
    29341 gcgagcgcat cgacctgttc aggtttcagt accgtacctg cgccaatcaa cgccttgtcg
    29401 ccatacgcat caacgatggc gggaatgctc tgctcccatt gtggggaatt cagcgggatt
    29461 tcaaccgcgt cgaacccggc gtcaatcacc gcgccaacat gcgccagcgc ctcgtcgggc
    29521 gtaatgccgc gcaaaatggc gatcagcggg agtttagttt gccactgcat gagcgatgct
    29581 ccttatacca gcctgaaatg ccgtgtcgcc cgccaccgcc gccacgtcgc aacccatcgc
    29641 ctgaaaggct tgctggtagc gcgcggtcag cgatgttccg gcgacaaggg tgatggcgtg
    29701 ttgatgggtc acatagtcgc gcatactggc aacctctgcg ccaatcaaca aaccagagag
    29761 aaattcgctg acctgttcgc ggggaagtgt tcccagcaca tgcgaggcgc gaacttcaaa
    29821 aagctgcggc aatatggcgg gcgcattaag gccgcgctca aggccagccg cgaaggcatc
    29881 ggcagagttt tcctgcggcg gcaaacctgc gccaatcaat gagtgattta acagtaaatg
    29941 atgtaattca ccggtcatca cggtgcgaaa atcgttgatt tgctggctat cggcctgcac
    30001 ccatttgcaa tgcgtaccgg gcataacata aagagaggaa ggagccagag ctcgcgcgcc
    30061 gatcaattgt gtttcttcgc cgcgcatcac attgtggtta tcgtcatgag agacgcataa
    30121 tccgggaata atccagatat tgtcgccaac agacgttaat tgttcgccaa tagacgaaaa
    30181 acgggcagga acagataaat acggagcaac tttccagccg acattgctgc cgaccattcc
    30241 tgccatcacc actggcgtat tttcttcacg ccagtcggtc gtgacttctg ctaacactgc
    30301 agccggagat tttccgttca ggcgcgtgac gcctgcttct gattgcctgc tctccaggca
    30361 gtggtcgccc tgataaagcc aggcgcgcag attggtcgat ccccagtcaa ttgcgatgta
    30421 gcgagctgtc atgtgatttc ctttaacctt cgtgtcgagc tggcaatcat ggtaagcgcc
    30481 gcctgctctg ccgcatcgcc gtcctgatgc cgtatcgcat cgaacagcgc cttatgttcc
    30541 tggagcgttt gcggcatgtt ggcctcatcg cccatccagg ttcgttcaaa aaccgcccgc
    30601 tgcagcgaac tgatcgcaat gctaagttgc tgtaacaccg ggttatgcac cgactgcagc
    30661 accgcctcgt ggtagcgaat atccgcttcg ttaaacgctt cgcggtcctg attgttggca
    30721 atcatctcgt tcagcgccga ttcaatctgc gccagatcgc tggaggtcgc gcgctctgct
    30781 gcccaacggg caatcgccgg ttccaccaga tttcgcactt cgctcatggc actgataagc
    30841 cgtgggtcgt agtcgttttc cagcacccat tgcagtacgt cagtgtcgag gtaattccac
    30901 tggttacgcg gtgccacaaa cgccccgcga taacgtttca tttcaatcag ccgcttcgcc
    30961 atcagcgaac ggaacacctc acggatgatg ttgcgcgagg ttgcaaactc ttcacagagc
    31021 tccgcctcag ccgcaagtgg cgagccaggt acgtatttgc cgtgaacgat ctgtttaccc
    31081 agcgtaatga caatgcgatc ggttttattg agagtcatgg agagtccttg tgctctgtat
    31141 gttcttctct actttacccc gatcgatgca taacgcggca actttgtagt accagcgtga
    31201 tgacgttcgc gtttgccgcg cgcgtaatgt agtacaaact tatattgttg cgctacaatt
    31261 tagatcaaaa aaagaacaac acataaaaaa atgacatgcg tcgggcagaa atctgaaaag
    31321 ggatatcagg cgctaaacag gagggaaaga agagtatgct ttcaacggct tagctactcg
    31381 tttaaaggat taatcatgaa gttgaatttt aagggatttt ttaaggctgc cggtttattc
    31441 ccactggcgc tgatgctttc aggctgtatc tcgtatgctc tggtttccca taccgcaaag
    31501 ggtagttcag gaaagtatca atcgcagtca gacaccatca ctgggctatc gcaggcaaaa
    31561 gatagtaatg gaacaaaagg ctatgttttt gtaggggaat cgctggatta ccttatcact
    31621 gatggtgccg atgacatcgt taagatgctc aatgatccag cacttaaccg gcacaatatt
    31681 caggttgccg atgacgcaag atttgtttta aatgcgggga aaaagaaatt taccggcaca
    31741 atatcgcttt actactactg gaataacgaa gaagaaaagg cactggcaac gcattatggt
    31801 tttgcctgtg gtgttcaaca ctgtaccagg tcactggaaa acctaaaagg cacaatccat
    31861 gagaaaaata aaaacatgga ttactcaaag gtgatggcgt tctaccatcc atttaaagtg
    31921 cgattttatg aatactattc acccagaggc attccggatg gtgtttccgc agcattactg
    31981 ccagtgactg ttacactgga catcattact gcaccgctgc aatttctggt tgtatatgca
    32041 gtaaaccaat aatcagtaag cgggcaaacg cgtttatgct gtttgcccgc ccacagatta
    32101 attcagcaca tacttctcaa tagcaaacgc cacgccatct tcaaggttgg attttgtgac
    32161 aaagttcgcc acttctttca ccgaaggaat agcgttatcc atcgccacac cgacgcctgc
    32221 atattcaatc attgcgatgt cgttttcctg atcgccaatc gccatgattt cttccggttt
    32281 aatacctaac acgtcggcca gtgatttcac acccgtacct ttgttaacgc gtttatcgag
    32341 gatttcgagg aagtacggcg cacttttcag cacggtatat ttctctttca cttcctgcgg
    32401 aatacgcgcg atagcctggt cgaggatggc gggttcatca atcatcatca ctttcaggaa
    32461 ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaattg tggcaacgaa
    32521 ggattcatgc accgtgtagt agctgatatc gcggttagcg gtgtacagcg tggtgcggtc
    32581 cagggcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac gatagtcgtc
    32641 atagctgaga gcggtttgcg ccacggtgct accatcagcg gccttctgta ccagcgcgcc
    32701 gttataagta atgcagtagt cgcccggctg ttccatatgc agctctttca ggtagttgtg
    32761 cacacctgca tacggacgac ccgtcgttag cacgacattc acgccacggg cgcgagctgc
    32821 ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa gggtgccatc
    32881 catatcgata gcaatgagtt tgatagccat gagttcccaa ggtagattag ttcctgaccc
    32941 atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg ggatgcaacg
    33001 cgggttgcat gctccccatt tgcttagcgg aagagtttcc ctttcagcag ttccatgcct
    33061 gcggaaagca gatcgttatt ggcttgtgct gacacttcac cttgcggtga gagcgcatca
    33121 ataatcttcg gcaattgctc tgccagtaaa ctggaagccg tactggtatc cacgccaagt
    33181 ttttgcccga gatcggacac cgcatttgtg ccgagcgccg actccagttg ctcgccgcta
    33241 actgattgat ttctctgttg attactcaac caggttgaga gaatggcccc taagccgcca
    33301 ctttgcagtt tttccagcag cacctgaatg ccaccctgct cctcaaccca acttaaaatc
    33361 gcctgatatt tccccgcatc gcctttcaga aaggcaccga caacttcatc aaaaagcccc
    33421 atgataatca cctgtaaagc gttacgtgtt gacccaaaaa gtatagatct gcgggatgat
    33481 aattgcggat tgcagaaata aaaagggcgg agatgatctc cgcccttttc ttatagcttc
    33541 ttgccggatg cggcgtgaac gccttacccg gcctacaaaa tcatgcaaaa tcaatatatt
    33601 gcaggagtct cgtaggacta ataagcgtag cgcatcaggc acgctcgcat ggttaacgcc
    33661 attaaatatc aatattcgcc gctttcaggg cgttctcttc aataaacgca cgacgcggtt
    33721 ctacggcgtc gcccatcagc gtggtgaaca actggtcagc ggcaatcgca tccttaacgg
    33781 taacgcgcag catacgacgg ctttccgggt ccatggtggt ttcccacagc tgttccgggt
    33841 tcatctcgcc cagaccttta taacgctgga tggagaggcc acgacgggac tctttcacca
    33901 gccagtccag cgcctgctcg aagctggcta ccggctgacg acgctcgcca cgttcgataa
    33961 acgcatcttc ttccagcaag ccacgcagtt tctcacccag cgtgcagata cgacgatatt
    34021 cgccaccggt gataaactcg tgatccagcg gatagtcagt atccacaccg tgggtacgca
    34081 cgcgaacaat cggctcgaac aggttttgtt cggcattggt gtggacatca aacttccact
    34141 ggctgccgtg ctgttctttg tcgttcagtt cgctgaccag cgcgttcacc cagcgggtaa
    34201 cggtctgctc atcagaaagg tcagcttccg tcaacgtcgg ctgatagata agctctttca
    34261 gcattgcttt cggataacga cgctccatgc ggttgatcat tttctgcgtc gcgttgtact
    34321 cagataccag tttctctaac gcttcgccag ccaatgccgg tgcactggcg ttggtgtgca
    34381 gcgttgcgcc gtccagcgcg atagagatct ggtactgatc catcgcttcg tcgtctttaa
    34441 tgtactgttc ctgcttgcct ttcttcactt tgtacagcgg cggctgagcg atgtagacgt
    34501 gaccgcgttc aacgatttcc ggcatctgac gatagaagaa ggtcaacagc agcgtacgaa
    34561 tgtgcgagcc gtcgacgtcc gcatcggtca tgatgatgat gctgtgataa cgcagtttgt
    34621 ccgggttgta ctcgtcacga ccgataccac agccaagcgc ggtgataagc gtcgccactt
    34681 cctgagaaga gagcatctta tcgaagcgcg ctttctcgac gttaaggatt ttacccttca
    34741 gcggcagaat cgcctggttc ttgcggttac gcccctgctt cgcagagccg cccgcggagt
    34801 ccccttccac caggtacagt tcggaaagcg ccggatcgcg ttcctggcag tctgccagtt
    34861 tgcccggcag gccagctaag tcgagcgcac ctttacggcg ggtcatttca cgcgcgcgac
    34921 gcgccgcttc acgggcacgg gcagcatcga taattttgcc aaccacgatt ttcgcatcag
    34981 ttgggttttc cagcaggtat tccgccagca gttcgttcat ctgctgttca accgccgatt
    35041 tcacctcaga agaaaccagt ttgtctttgg tctgggagga gaatttcggg tccggcactt
    35101 ttacggaaac gaccgcaatc aggccttcac gcgcatcgtc accggtggcg ctgactttgg
    35161 cttttttgct gtagccttct ttgtccatgt aggcattcag ggtacgggtc atcgccgcac
    35221 ggaagcctgc caggtgagta ccgccgtcac gctgcggaat gttgttggta aagcagtaga
    35281 tgttttcctg gaagccatcg ttccactgca gcgccacttc aacgccaata ccgtcttttt
    35341 cggtggagaa gtagaagata tttgggtgga tcggcgtttt gttcttgttc agatattcaa
    35401 cgaacgcttt gatgccgcct tcatagtgga agtggtcttc tttgccgtca cgcttgtcgc
    35461 gcagacgaat ggaaacgccg gagttgagga acgacaactc acgcagacgt ttcgccagaa
    35521 tttcatattc gaactcggtc acattggtga aggtttcgag gctgggccag aaacgcacca
    35581 tggtgccggt tttttcagtc tcgccggtaa ccgccagcgg agcctgcggt acaccgtgtt
    35641 cgtagatctg acggtgaatt ttaccctcgc gctgaataac cagctccagt ttttgcgaca
    35701 gggcgtttac taccgaaaca ccaacgccgt gcagaccgcc ggacacttta taggagttat
    35761 cgtcaaattt accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc gatacgccct
    35821 cttccgggtg aataccggtc ggaatgccgc gcccgtcatc ctgtacagag acagagttat
    35881 cggcgtgaat ggtgacgata atttctttac agtgacccgc gagcgcttcg tcgatagcgt
    35941 tatctaccac ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg tcgccgatat
    36001 acatacccgg gcgcttacgc accgcatcca gccctttcag gactttgata ctggaggagt
    36061 cataagaatt cgacatcaac gtttctcgct catttatact tgggttaatc cgttatttta
    36121 cccttttcca cggtaaacat cttcgaattt tcgtccgaca tgtctataac gtgttcagca
    36181 ctgatcgcgc tgacaaagac ctgtgattgc gtcgctttta agcggctggc aagcaacccg
    36241 cgacgctcat catcaagctc agaggcaaaa tcatctatca ggtagagaca ccgccgcccg
    36301 ctttcacgag tgaggaactc tccttgcgcc agacgtaagg cgcacatcaa cagcttaagc
    36361 tgcccacgcg ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg taagtccgct
    36421 ttatgcgggc catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt acgttccagc
    36481 acctcagcgt actctgtctc tttctcccag ccgcgctgga aagagaaagt cagagaaaac
    36541 tcagggagaa attgcttaca ggtatcatcc atatcggccg cgataccggc gctatactcc
    36601 gcgcgccagg tgctgatttg ctccgccagc gggatcagtt ctttatccca cgggcgtagc
    36661 tgttcgtaac gagtcacctg gcgcagcgcc gcattgcgct gcttgagcaa tcgcttgaga
    36721 ttgctccagg cggtgaaaaa tccaggttcg ttgtgaaagc atccccagtc gaggaatgct
    36781 cttctgtatt tggggccgcc gttgagtaaa gtaaaccctt ctggcgttat caactgcatc
    36841 ggcatcaggt gcgccagttc cgcgacttta tgcccgtccg taccgtcaat acggactttg
    36901 ctgtcgccct gtttgtcttt ggttaagcca atcgccgtct cgcgctcttc gccctgtaat
    36961 cgcccgtgga gaacaaatgc ctcctgctca tggcgaatga cgcgaccaat ctgcaaactg
    37021 cgaaacgccc gaccatggcc gagcgtatag atggcttcca gcacgctggt tttgccactg
    37081 ccgttggcac ctaccagaaa gttaaagccg ggagataaag cgagatccgc ggtttcaata
    37141 ttgcggaaat cgcggatcaa caagcgggtg agggacatta cagtctcatt ggcatgacaa
    37201 cataggcagc cgactgtgat gcggcatctt caatctgcac gctggaaacc gaatcggtca
    37261 gcatcatgcg gacgttttcg catttcagcg cgttcagaac atccagcaca tagctgacgt
    37321 tgaagccgat ttccatctcc gcaccgctat aggtaacgtc gaggatctct tccgcttctt
    37381 cctgttccgg gttgttggca gtaattttca gctggttttc gctgacatac aggcgcacac
    37441 cgcggaattt ctcgttagag agaatcgccg cgcgagcaaa cgcctgcttg agcagatcgc
    37501 agcctgcttc cagatgtttg tccgggttct tcggcagaac gcgacggtaa tccgggaagc
    37561 gaccatccac cagtttcgag gtgaaaataa agtcgccaac gtgggcacga atgttattgc
    37621 tgccaatctg cacacgcagc agattgtcgc cgccgtcgag catacgcatc agttcaatca
    37681 cgcctttacg cggtacgatc accgaatggc ttggcaaaga ttgaccaatt ggcattgaac
    37741 agaccgccag acggtggccg tcggttgcca cggtgcgcag ttcttcacct tcggtttcaa
    37801 acagcatacc gtttaagtaa tagcgaacat cctgatgcgc catcgaaaac tgggttgctt
    37861 caatcagacg cttcatcgtc gcctgcggca gggtaaattc gacttcgctc tgccagtcat
    37921 cgaggttcgg gaaatccgcc gctggcaggg tagacagcga aaaacggcta cgcccggagc
    37981 gcaccagcat ccgttcacct tccagctgca cggcaatttc cgcgccttca ggcagaccac
    38041 ggcagatatc aaagaatttg cgcgccggaa cggtcgtcgc tcctggctcg tgtggctgaa
    38101 ccagcgcaac gcgtgccacc atttccatct cgagatcagt accggtcagc gacaacgtac
    38161 cgtcagcaac ctgtaacagc agattaccga gaatcggtag cgtaggacga ccacctaacg
    38221 gaccgctcac ctgttgtagc ggttttaata aatgctcacg ttctacggta aatttcatag
    38281 gtttacgatg acaatgttct gattaaattt gaaaaatctt ctttgatatc gtggctctct
    38341 tcacgcaact gctcgatctt acggcaggca tgaagcaccg tcgtgtggtc acgcccacca
    38401 aacgcatcgc caatctctgg cagactgtgg ttagtcagct ctttcgctag cgccatcgcc
    38461 atctggcgcg gacgcgccac cgagcgggat cgacgcttgg aaaggagatc cgcgactttg
    38521 atcttgtagt actccgccac cgtcttctga atattgtcga tggtgaccag tttttcctgc
    38581 aatgccagca agtcgcgcag cgcctcacgc acgaagtcga tggtgatcgc ccgtccggta
    38641 aagttggcgt tagcaataac gcggttcagc gccccttcca gctcacgtac gttagatcgt
    38701 agacgcttgg cgataaagaa cgccacttca cccggcaaac gaatgtcatt ttcgtcggcc
    38761 tttttcatca ggatcgccac acgggtttcc agctctggcg gttcgatcgc cacagtcagt
    38821 ccccaaccga agcgggattt caaacgatcc tcaacgccgt tgatctcttt cggatagcga
    38881 tccgaggtga gaatgatctg ttgattacct tccagcaggg cgttgaaggt gtggaaaaac
    38941 tcttcctgag atcgttcttt attagcaaaa aactgaatat cgtcgatcag cagtgcatct
    39001 acggaacggt agtagcgttt aaactcttcg atcgcgttgt tttgcagggc tttaaccatg
    39061 tcctgaacaa agcgctcgga gtgcatataa accactttgg cattcggctt gcgcgccata
    39121 atgccgttac ccaccgcatg cagcaggtga gttttaccca gacccgtgcc gccataaagg
    39181 aacaacgggt tataggcacc gccagggtta tccgccacct ggcaagccgc cgcgcgcgcc
    39241 agttggttag atttaccttc aacgaagtta tcaaacgtgt gtttgacgtt tacgttagaa
    39301 cgataggtcg gttctgccgg ggccgggacg ttatcccaac ctgagcgcgt agaaggcgca
    39361 gcacgttgcg gctgcgtttg cgccacctgt gcaggggccg cgacgttgct cgtcactgcc
    39421 gcttgtggcg tttgcgtcac cggtttggtg ccgacttcaa aacgcagctg tggggcatcc
    39481 gctccgcaga aactggttag cagtccattg atattattaa ggtacttgtc ccgtacccaa
    39541 tcgaggacaa aacggtttgg cgcgtacagg gccagcgtgt tatcgctcag ttccgcctgc
    39601 aatgggcgta tccacatact gaattctgtg gctggtaact catcctgcaa tcgggcaaga
    39661 cactgctgcc aaagcgaaag tgacacggcg gactccactc gaacaaaagt cgataatgac
    39721 taaggctgaa acattcatga ttgttgacgt acgtcgaaaa gaccctgctt gagggtgacg
    39781 cacgaaccgc tgtctgcggt tatatgcccg atcaagatcc tgcaaaacga tcgggaccgc
    39841 ggatcatagc ctaaactgcg caagagatct tctgtttctc acagattttc cctatttatc
    39901 cacaggactt tccagaactc gctaagtgta aacgatcctg ccgcgaggcg ggcacgattt
    39961 acgccgcata ttggaaaatt taatgaccat agacaaaaat tggcttaatc gatctaataa
    40021 agatccagga cgatccttgc gctttaccca tcagcccgta taatcctcca cccggcgcgc
    40081 catgctggtt tccactggtg tgaggtcgta cattttccct gcgaaaaggt gcggaaaagc
    40141 gcggtaaata aggaaagaga attgactccg gtgtgtacaa ttattacaat ccggcctctt
    40201 taatcaccca tggcttcggt gtccatcgtt tcatttttcg gcggatatcc aataaagcca
    40261 ttgaatttat tcaagtttag gtagaaatcg ccatgaaacg cacttttcaa ccgtctgtac
    40321 tgaagcgcaa ccgttctcac ggcttccgtg ctcgtatggc tactaaaaat ggtcgtcagg
    40381 ttctggcacg tcgtcgtgct aaaggccgcg ctcgtctgac cgtttctaag taataaagct
    40441 aacccctgag tggttaagct cgcatttccc agggagttac gcttgttaac tcccagtcaa
    40501 ttcacattcg tcttccagca gccacaacgg gctggcacgc cgcaaattac cattctcggc
    40561 cgcctgaatt cgctggggca tccccgtatc ggtcttacag tcgccaagaa aaacgttcga
    40621 cgcgcccatg aacgcaatcg gattaaacgc ctgacgcgtg aaagcttccg tctgcgccaa
    40681 catgaactcc cggctatgga tttcgtggtg gtggcgaaaa aaggggttgc cgacctcgat
    40741 aaccgtgctc tctcggaagc gttggaaaaa ttatggcgcc gccactgtcg cctggctcgc
    40801 gggtcctgat agccctcatt cgggtctatc aacgcctgat tagtccgcta ctcgggccgc
    40861 attgtcgttt cactccaacc tgttcaagct acggaattga ggcattgcgc aggtttggag
    40921 tgataaaagg cagttggttg acggtgaaac gcgtattaaa atgccaccct ttacaccctg
    40981 gtggtgacga tcccgtcccg cccggaccat ttgataccag agaacactaa cgatggattc
    41041 gcaacgcaat cttttagtca tcgctttgct gttcgtgtct ttcatgatct ggcaagcctg
    41101 ggagcaggat aaaaacccgc aacctcaggc ccaacagacc acgcagacaa cgaccaccgc
    41161 agcgggtagc gccgccgacc agggcgtacc ggccagtggc caggggaaac tgatctcggt
    41221 taagaccgac gtgcttgatc tgaccatcaa cacccgtggt ggtgatgttg agcaagctct
    41281 gctgcctgct tacccgaaag agctgaactc tacccagccg ttccagctgt tggaaacttc
    41341 accgcagttt atttatcagg cacagagcgg tctgaccggt cgtgatggcc cggataaccc
    41401 ggctaacggc ccgcgtccgc tgtataacgt tgaaaaagac gcttatgtgc tggctgaagg
    41461 tcaaaacgaa ctgcaggtgc cgatgacgta taccgacgcg gcaggcaaca cgtttaccaa
    41521 aacgtttgtc ctgaaacgtg gtgattacgc tgtcaacgtc aactacaacg tgcagaacgc
    41581 tggcgagaaa ccgctggaaa tctcctcgtt tggtcagttg aagcaatcca tcactctgcc
    41641 accgcatctc gataccggaa gcagcaactt cgcactgcac accttccgtg gcgcggcgta
    41701 ctccacgcct gacgagaagt atgagaaata caagttcgat accattgccg ataacgaaaa
    41761 cctgaacatc tcttcgaaag gtggttgggt ggcgatgctg caacagtatt tcgcgacggc
    41821 gtggatcccg cataacgacg gtaccaacaa cttctatacc gctaatctgg gtaacggcat
    41881 cgtcgctatc ggctataaat ctcagccggt actggttcag cctggtcaga ctggcgcgat
    41941 gaacagcacc ctgtgggttg gcccggaaat ccaggacaaa atggcagctg ttgctccgca
    42001 cctggatctg accgttgatt acggttggtt gtggttcatc tctcagccgc tgttcaaact
    42061 gctgaaatgg atccatagct ttgtgggtaa ctggggcttc tccattatca tcatcacctt
    42121 tatcgttcgt ggcatcatgt acccgctgac caaagcgcag tacacctcca tggcgaagat
    42181 gcgtatgctg cagccgaaga ttcaggcaat gcgtgagcgt ctgggcgatg acaaacagcg
    42241 tatcagtcag gaaatgatgg cgctgtacaa agctgagaag gttaacccgc tgggcggctg
    42301 cttcccgctg ctgatccaga tgccaatctt cctggcgttg tactacatgc tgatgggttc
    42361 cgttgaactg cgtcaggcac cgtttgcact gtggatccac gacctgtcgg cacaggaccc
    42421 gtactacatc ctgccgatcc tgatgggcgt aacgatgttc ttcattcaga agatgtcgcc
    42481 gaccactgtg accgacccga tgcagcagaa gatcatgacc tttatgccgg tcatcttcac
    42541 cgtgttcttc ctgtggttcc cgtcaggtct ggtactgtac tatatcgtca gcaacctggt
    42601 aaccattatt cagcagcagc tgatttaccg tggtctggaa aaacgtggcc tgcatagccg
    42661 cgagaagaaa aaatcctgat tcggtgagtt ttcgctaaaa taagggcggt cagttgaccg
    42721 ccttttttct tttcgtaggg cggataagca ccgcgcatcc gccacacaaa gcaacaggaa
    42781 catcatgagc gataatgaca ctatcgtagc ccaggccacg cctccgggac gtggcggcgt
    42841 tggcatcctg cgcatctccg gcttcaaagc ccgtgaagtt gccgaaaccg tgctgggtaa
    42901 actgcctaag ccgcgctacg ccgattatct tccgtttaaa gacgccgacg gcagcgtgct
    42961 cgatcagggg attgcgctat ggttccctgg cccgaactcg ttcaccggcg aagatgtgct
    43021 ggaactgcaa ggtcatggcg gtccggtgat cctcgacctg ctgttaaaac gcattctgac
    43081 cattcccggc ctgcggattg ctcgccctgg tgagttttcc gaacgcgcgt ttcttaacga
    43141 taaacttgac ttagcccagg ccgaggcgat tgccgatctt atcgacgcca gttcggaaca
    43201 ggcggcccgt tcggcactta actcgctgca aggcgcattc tccgcacggg ttaatcatct
    43261 ggtagaagcc ctcacccact tgcgcattta cgtcgaagcg gcaattgatt tccctgatga
    43321 agagatcgat ttcctctccg acggcaaaat tgaagcccag cttaatgacg tgatggccga
    43381 tctcgatgca gtgcgtgctg aagcacgtca gggtagtttg ttgcgcgaag ggatgaaagt
    43441 agtgattgcc ggacgtccta atgccggtaa atcgagcctg ttaaacgcgc tggcgggccg
    43501 tgaagcggca atcgtaaccg atatcgccgg aaccacgcgt gacgtgctgc gtgagcatat
    43561 ccacattgac ggaatgccgc tgcatatcat cgacactgcc gggctacgtg aggccagtga
    43621 cgaagtggaa cgtattggta tcgagcgcgc gtggcaggaa attgaacagg ccgaccgcgt
    43681 gctgtttatg gtcgatggca ccacgacaga cgccgttgat ccggcagaga tctggccgga
    43741 atttatcgcc cgtctgcccg cgaaactgcc gatcaccgtg gtacgcaata aagccgatat
    43801 caccggcgaa acgctgggaa tgagtgaagt gaacggtcac gcgttaattc gtctctcggc
    43861 aaggactggt gaaggcgtgg acgtgctgcg taaccatctc aaacagagca tgggctttga
    43921 caccaatatg gaaggcggct tcctggcgcg ccgtcgccac ttacaggcgc tggaacaggc
    43981 agcggagcat ttgcagcaag gtaaagcgca actgttggga gcctgggcag gtgaactgct
    44041 ggcggaagag ttgcgcctgg cacagcagaa cttaagcgaa atcaccgggg aatttacttc
    44101 agacgacctg ctggggcgga ttttctccag cttctgtatt ggtaagtaac cgcgcttacg
    44161 aagccgtatt ctgactgtca gatgcggctt cgcttcattg ttagcactcc tgttatgcct
    44221 caaccctttt ttaaacatta aaattcttac gtaatttata atctttaaaa aaagcattta
    44281 atactgctcc ccgaacgatt gtgattcgat tcacatttaa acaatttcag aatagacaaa
    44341 aatt
//
LOCUS       DAZPEU010000015        44138 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPEU010000015 DAZPEU010000000
VERSION     DAZPEU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44138
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.015"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..794
                     /locus_tag="ACHU2L_000709"
     CDS             <1..794
                     /locus_tag="ACHU2L_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1355692.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="ACHU2L_000710"
     CDS             805..1896
                     /locus_tag="ACHU2L_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1355693.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="ACHU2L_000711"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="ACHU2L_000711"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR1355694.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="ACHU2L_000712"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="ACHU2L_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR1355695.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="ACHU2L_000713"
     CDS             4858..5253
                     /locus_tag="ACHU2L_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR1355696.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="ACHU2L_000714"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="ACHU2L_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR1355697.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="dolP"
                     /locus_tag="ACHU2L_000715"
     CDS             5873..6448
                     /gene="dolP"
                     /locus_tag="ACHU2L_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR1355698.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="ACHU2L_000716"
     CDS             complement(6562..7602)
                     /locus_tag="ACHU2L_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1355699.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="ACHU2L_000717"
     CDS             complement(7675..8310)
                     /locus_tag="ACHU2L_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR1355700.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="ACHU2L_000718"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="ACHU2L_000718"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR1355701.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="ACHU2L_000719"
     CDS             complement(8936..9379)
                     /locus_tag="ACHU2L_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR1355702.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="ACHU2L_000720"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="ACHU2L_000720"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR1355703.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="ACHU2L_000721"
     CDS             complement(9719..10222)
                     /locus_tag="ACHU2L_000721"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1355704.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="ACHU2L_000722"
     CDS             complement(10216..10740)
                     /locus_tag="ACHU2L_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR1355705.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="ACHU2L_000723"
     CDS             10949..11944
                     /locus_tag="ACHU2L_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR1355706.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="ACHU2L_000724"
     CDS             11953..12831
                     /locus_tag="ACHU2L_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR1355707.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="ACHU2L_000725"
     CDS             12912..13919
                     /locus_tag="ACHU2L_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1355708.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="ACHU2L_000726"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="ACHU2L_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR1355709.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="ACHU2L_000727"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="ACHU2L_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR1355710.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="ACHU2L_000728"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="ACHU2L_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR1355711.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="ACHU2L_000729"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="ACHU2L_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR1355712.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="ACHU2L_000730"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="ACHU2L_000730"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR1355713.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="ACHU2L_000731"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="ACHU2L_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR1355714.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="ACHU2L_000732"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="ACHU2L_000732"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR1355715.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="ACHU2L_000733"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="ACHU2L_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR1355716.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="ACHU2L_000734"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="ACHU2L_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR1355717.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="ACHU2L_000735"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="ACHU2L_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR1355718.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="ACHU2L_000736"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="ACHU2L_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR1355719.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="ACHU2L_000737"
     tRNA            complement(27653..27729)
                     /locus_tag="ACHU2L_000737"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="ACHU2L_000738"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="ACHU2L_000738"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR1355720.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31054)
                     /locus_tag="ACHU2L_000739"
                     /pseudo
     CDS             complement(29428..31054)
                     /locus_tag="ACHU2L_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31513..31599)
                     /locus_tag="ACHU2L_000740"
     tRNA            complement(31513..31599)
                     /locus_tag="ACHU2L_000740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="ACHU2L_000741"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="ACHU2L_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR1355721.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="ACHU2L_000742"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="ACHU2L_000742"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR1355722.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="ACHU2L_000743"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="ACHU2L_000743"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR1355723.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="ACHU2L_000744"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="ACHU2L_000744"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR1355724.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="ACHU2L_000745"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="ACHU2L_000745"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR1355725.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="ACHU2L_000746"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="ACHU2L_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR1355726.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="ACHU2L_000747"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="ACHU2L_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1355727.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="ACHU2L_000748"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="ACHU2L_000748"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1355728.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39901..40002
                     /locus_tag="ACHU2L_000749"
                     /pseudo
     CDS             39901..40002
                     /locus_tag="ACHU2L_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="ACHU2L_000750"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="ACHU2L_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR1355729.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="ACHU2L_000751"
     CDS             complement(41211..42176)
                     /locus_tag="ACHU2L_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1355730.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="ACHU2L_000752"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="ACHU2L_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR1355731.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="ACHU2L_000753"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="ACHU2L_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR1355732.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="ACHU2L_000754"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="ACHU2L_000754"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR1355733.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
BASE COUNT        10785 a        12043 c        10804 g        10506 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccaccattac cgtcatggtg cggtaaaaga agacgttgcg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgta
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa caccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgttcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctgctgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc tacagatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgtattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggcg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagcaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caggctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagtgagag cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgcgct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tcagccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaagtt cgccaggtca gcaccggaga aaccaggagt accacgagca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatcg ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatccc
//
LOCUS       DAZPEU010000016        42505 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPEU010000016 DAZPEU010000000
VERSION     DAZPEU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.016"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..751
                     /locus_tag="ACHU2L_000755"
     CDS             <1..751
                     /locus_tag="ACHU2L_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355734.1"
                     /translation="ELMRKRLAFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELM
                     DKYFTTLLKEISNQPLEDWDIIISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHS
                     ESEMRGLFSSLAELYSFGNDTASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLI
                     KEEMLGMHNLILLENICMQKNAECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKI
                     YHKLEIKPEPEQQVLIQSLYDRASGSASATQ"
     gene            complement(811..1302)
                     /gene="yajQ"
                     /locus_tag="ACHU2L_000756"
     CDS             complement(811..1302)
                     /gene="yajQ"
                     /locus_tag="ACHU2L_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR1355735.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1470..2381
                     /gene="panE"
                     /locus_tag="ACHU2L_000757"
     CDS             1470..2381
                     /gene="panE"
                     /locus_tag="ACHU2L_000757"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR1355736.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2344..2934
                     /gene="yajL"
                     /locus_tag="ACHU2L_000758"
     CDS             2344..2934
                     /gene="yajL"
                     /locus_tag="ACHU2L_000758"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR1355737.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(2988..4436)
                     /gene="thiI"
                     /locus_tag="ACHU2L_000759"
     CDS             complement(2988..4436)
                     /gene="thiI"
                     /locus_tag="ACHU2L_000759"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR1355738.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4642..4884
                     /gene="xseB"
                     /locus_tag="ACHU2L_000760"
     CDS             4642..4884
                     /gene="xseB"
                     /locus_tag="ACHU2L_000760"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR1355739.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4884..5783
                     /gene="ispA"
                     /locus_tag="ACHU2L_000761"
     CDS             4884..5783
                     /gene="ispA"
                     /locus_tag="ACHU2L_000761"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR1355740.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5808..7670
                     /gene="dxs"
                     /locus_tag="ACHU2L_000762"
     CDS             5808..7670
                     /gene="dxs"
                     /locus_tag="ACHU2L_000762"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR1355741.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7725..8642
                     /gene="yajO"
                     /locus_tag="ACHU2L_000763"
     CDS             7725..8642
                     /gene="yajO"
                     /locus_tag="ACHU2L_000763"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR1355742.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(8753..9271)
                     /gene="pgpA"
                     /locus_tag="ACHU2L_000764"
     CDS             complement(8753..9271)
                     /gene="pgpA"
                     /locus_tag="ACHU2L_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR1355743.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9249..10226)
                     /gene="thiL"
                     /locus_tag="ACHU2L_000765"
     CDS             complement(9249..10226)
                     /gene="thiL"
                     /locus_tag="ACHU2L_000765"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR1355744.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(10304..10723)
                     /gene="nusB"
                     /locus_tag="ACHU2L_000766"
     CDS             complement(10304..10723)
                     /gene="nusB"
                     /locus_tag="ACHU2L_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR1355745.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10743..11213)
                     /gene="ribE"
                     /locus_tag="ACHU2L_000767"
     CDS             complement(10743..11213)
                     /gene="ribE"
                     /locus_tag="ACHU2L_000767"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR1355746.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11302..12405)
                     /gene="ribD"
                     /locus_tag="ACHU2L_000768"
     CDS             complement(11302..12405)
                     /gene="ribD"
                     /locus_tag="ACHU2L_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR1355747.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12409..12858)
                     /gene="nrdR"
                     /locus_tag="ACHU2L_000769"
     CDS             complement(12409..12858)
                     /gene="nrdR"
                     /locus_tag="ACHU2L_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR1355748.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13009..13548
                     /locus_tag="ACHU2L_000770"
     CDS             13009..13548
                     /locus_tag="ACHU2L_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1355749.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13846..14730
                     /gene="tsx"
                     /locus_tag="ACHU2L_000771"
     CDS             13846..14730
                     /gene="tsx"
                     /locus_tag="ACHU2L_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR1355750.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14907..15254)
                     /gene="yajD"
                     /locus_tag="ACHU2L_000772"
     CDS             complement(14907..15254)
                     /gene="yajD"
                     /locus_tag="ACHU2L_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR1355751.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15383..16354)
                     /gene="secF"
                     /locus_tag="ACHU2L_000773"
     CDS             complement(15383..16354)
                     /gene="secF"
                     /locus_tag="ACHU2L_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR1355752.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16365..18212)
                     /gene="secD"
                     /locus_tag="ACHU2L_000774"
     CDS             complement(16365..18212)
                     /gene="secD"
                     /locus_tag="ACHU2L_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR1355753.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18240..18572)
                     /gene="yajC"
                     /locus_tag="ACHU2L_000775"
     CDS             complement(18240..18572)
                     /gene="yajC"
                     /locus_tag="ACHU2L_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR1355754.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18595..19722)
                     /gene="tgt"
                     /locus_tag="ACHU2L_000776"
     CDS             complement(18595..19722)
                     /gene="tgt"
                     /locus_tag="ACHU2L_000776"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR1355755.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLLEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19777..20847)
                     /gene="queA"
                     /locus_tag="ACHU2L_000777"
     CDS             complement(19777..20847)
                     /gene="queA"
                     /locus_tag="ACHU2L_000777"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR1355756.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20940..21513
                     /gene="acpH"
                     /locus_tag="ACHU2L_000778"
                     /pseudo
     CDS             20940..21513
                     /gene="acpH"
                     /locus_tag="ACHU2L_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(21518..23335)
                     /gene="malZ"
                     /locus_tag="ACHU2L_000779"
     CDS             complement(21518..23335)
                     /gene="malZ"
                     /locus_tag="ACHU2L_000779"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR1355757.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23491..24864)
                     /gene="proY"
                     /locus_tag="ACHU2L_000780"
     CDS             complement(23491..24864)
                     /gene="proY"
                     /locus_tag="ACHU2L_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR1355758.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24940..26259)
                     /gene="brnQ"
                     /locus_tag="ACHU2L_000781"
     CDS             complement(24940..26259)
                     /gene="brnQ"
                     /locus_tag="ACHU2L_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR1355759.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26666..27961)
                     /gene="phoR"
                     /locus_tag="ACHU2L_000782"
     CDS             complement(26666..27961)
                     /gene="phoR"
                     /locus_tag="ACHU2L_000782"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR1355760.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28019..28708)
                     /gene="phoB"
                     /locus_tag="ACHU2L_000783"
     CDS             complement(28019..28708)
                     /gene="phoB"
                     /locus_tag="ACHU2L_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR1355761.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28898..30100
                     /gene="sbcD"
                     /locus_tag="ACHU2L_000784"
     CDS             28898..30100
                     /gene="sbcD"
                     /locus_tag="ACHU2L_000784"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR1355762.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            30097..33240
                     /gene="sbcC"
                     /locus_tag="ACHU2L_000785"
     CDS             30097..33240
                     /gene="sbcC"
                     /locus_tag="ACHU2L_000785"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR1355763.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33366..34547
                     /gene="araJ"
                     /locus_tag="ACHU2L_000786"
     CDS             33366..34547
                     /gene="araJ"
                     /locus_tag="ACHU2L_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR1355764.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(34690..35598)
                     /gene="mak"
                     /locus_tag="ACHU2L_000787"
     CDS             complement(34690..35598)
                     /gene="mak"
                     /locus_tag="ACHU2L_000787"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR1355765.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            35723..36634
                     /gene="rdgC"
                     /locus_tag="ACHU2L_000788"
     CDS             35723..36634
                     /gene="rdgC"
                     /locus_tag="ACHU2L_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR1355766.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36712..37167)
                     /locus_tag="ACHU2L_000789"
     CDS             complement(36712..37167)
                     /locus_tag="ACHU2L_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355767.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37164..37499)
                     /locus_tag="ACHU2L_000790"
                     /pseudo
     CDS             complement(37164..37499)
                     /locus_tag="ACHU2L_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(37654..37938)
                     /gene="ppnP"
                     /locus_tag="ACHU2L_000791"
     CDS             complement(37654..37938)
                     /gene="ppnP"
                     /locus_tag="ACHU2L_000791"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR1355768.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38010..38687)
                     /locus_tag="ACHU2L_000792"
     CDS             complement(38010..38687)
                     /locus_tag="ACHU2L_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR1355769.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(38945..39136)
                     /gene="yaiA"
                     /locus_tag="ACHU2L_000793"
     CDS             complement(38945..39136)
                     /gene="yaiA"
                     /locus_tag="ACHU2L_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR1355770.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39186..39710)
                     /gene="aroL"
                     /locus_tag="ACHU2L_000794"
     CDS             complement(39186..39710)
                     /gene="aroL"
                     /locus_tag="ACHU2L_000794"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR1355771.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(39893..40351)
                     /locus_tag="ACHU2L_000795"
     CDS             complement(39893..40351)
                     /locus_tag="ACHU2L_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR1355772.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            40471..41280
                     /gene="proC"
                     /locus_tag="ACHU2L_000796"
     CDS             40471..41280
                     /gene="proC"
                     /locus_tag="ACHU2L_000796"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR1355773.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41297..42412)
                     /gene="adrA"
                     /locus_tag="ACHU2L_000797"
                     /gene_synonym="dgcC"
     CDS             complement(41297..42412)
                     /gene="adrA"
                     /locus_tag="ACHU2L_000797"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR1355774.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        10707 a        11356 c        10607 g         9835 t
ORIGIN      
        1 ggaattgatg agaaaacgtc tggcgtttct gatgacagca ccagaatacg cttgttataa
       61 tagtcagtat tgttatgaag acaaagtcag cgcattaaaa atcctgtatc gtcatcatga
      121 caatgagcta atggacaaat atttcacgac tcttcttaaa gagatcagta atcaaccatt
      181 agaagactgg gatataataa taagtaatac tggtgaaaaa tttagtgaag tgggtctaac
      241 caaatatgct caacatctcg catctttcgc tgaagaattt tatataaaag agcaaaaatc
      301 acattctgaa agtgaaatga gaggactatt ttcatcactt gccgaattat atagttttgg
      361 taatgatact gccagcgcaa accgagtgtt ccatcagcat gtccctgcct tcaatacaga
      421 tcatatgatt caatatttta taaatgccaa agaatggaac aatgccaggg aattattgat
      481 aaaagaggaa atgttgggta tgcataatct gatattgttg gagaatattt gtatgcaaaa
      541 aaatgcagaa tgcatggcac acattacctt tacgcttaat aaattaacaa cacaaccagc
      601 aataacaaaa atagatagcg tgggtaatga gcaactttat cagattggca aaatctacca
      661 taagttggaa attaaacctg agccagagca acaggtttta attcagtcgc tttatgaccg
      721 ggcatcagga agcgcctctg caactcaata aatgcatggt caaaagggcc accacggtgg
      781 ccctgacaat cagtagctat atccagaaag ttaatcgcgg aagtttttga actggaacgg
      841 ctgaccgaga tcgccaccgc gcaccatcgc catgacagcc tgcaaatcat cacgagattt
      901 gccagtcacg cggatctcat cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt
      961 gatcatcttg acgatttttt tctgagtggc gctctcaatg ccctgtttca gtttcgcttc
     1021 cacaaaccag gttttaccgc tatgaacgat attttccggt acatccagtg aactgccttc
     1081 aatgccgcgc ttcagcagct tggcacgcag aatatccagc aactgattga cctggaagtc
     1141 ggactcgctc aacactttga tggttttgct ggcgtcgttc agctcaaatg aggcttcaac
     1201 gttacggaag tcaaaacggg actccacttc gcgactcgcg ttatcgaccg cgttacgtgc
     1261 ttcctgaaga tcaacttcag agacaatatc gaaagatggc atcttttcct ctcccttcat
     1321 ttttgatgcg aagcataata cctgcaaagt taaataacca acagtcctgc accaggacgt
     1381 atcccttaat tgtgcatagt cgcaacagca tgcacaatta agggatagcc ttattgaaca
     1441 cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac cgtattggga tgcggtgcct
     1501 tagggcaatt atggcttaca gcactttgta aacagggtca tgaagttcag ggctggctgc
     1561 gtgtaccgca accttattgt agtgtgaatc tggttgagac agatggttcg atatttaacg
     1621 aatcgctgac cgccaacgat cccgattttc tcgccaccag cgatctgctc ctggtgacgc
     1681 tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc atccacactg cctgtaacta
     1741 cgccaatact gttaattcac aacggcatgg gcaccatcga agaattgcaa aacattcagc
     1801 agccattact gatgggcacc accacccatg ccgcccgccg cgacggcaat gtcattattc
     1861 atgtggcaaa cggaatcacg catatcggtc cggcacggca acaggatggc gattacagtt
     1921 atctggcgga tattttgcaa accgtgttgc ctgacgttgc ctggcataac aatattcgcg
     1981 ccgagctgtg gcgcaagctg gccgtcaact gcgtgattaa tccactgact gccatctgga
     2041 attgcccgaa cggtgaatta cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag
     2101 tcgcggcagt gatcgaacgc gaagggcatc atacttcagc ggaagatttg cgtgattacg
     2161 tgatgcaggt gattgatgcc acagcagaaa atatctcgtc gatgttgcag gatatccgcg
     2221 cgctgcgcca caccgaaatc gactatatca atggttttct cttacgccgc gcccgcgcgc
     2281 atgggattgc cgtaccggaa aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg
     2341 aatatgagcg catcggcact ggtttgcctc gcccctggta gtgaagagac tgaagccgtc
     2401 accacgatca acctcttggt tcgcggcggt atcaaagtca ccactgccag cgtcgacagc
     2461 gatggcaacc tggcaattac ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg
     2521 gtcgaagtgg ctgatggcga atatgacgtg atcgtgctgc ctggtggcat taaaggcacg
     2581 gagtgttttc gcgatagcac tctgctggtt gaaaccgtta aacagttcca ccgttccggg
     2641 cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt
     2701 ccgattggca atatgaccgg cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg
     2761 caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt
     2821 acagctatcg actttggtct gaaaattatc gacctgctgg ttgggcgcga gaaagcccat
     2881 gaagtggcat cacaactggt gatggcggca gggatttata attattacga gtagtgtcgg
     2941 atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat
     3001 accttcacat tgttaaagcc ttgctcgcgc agatagagcg cctgcagacg gctcatcacc
     3061 ccgcgctcac accacagcag ccaggttttg ttctggtcga gatcgccaaa tttggtgctc
     3121 agtttataga acggcagaga aaccacatca atcccttcga ctttcagtgg cttatcttcc
     3181 tgttcatcga cagaacggat atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt
     3241 tccacttcca ccacttcctg ctcggtctgc tgggcgattt cgcggatatc aacgttattc
     3301 gcttcctcaa ccactttatc gagaatactg aagtcgaact tctcttcttc cgcttcaatc
     3361 ttcgatttaa ccgctttcac cgtcgggctt ttggagatca cgccacaata ttccggcatc
     3421 gtgcgagcaa agtcttcggt gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg
     3481 taagaaatca gcggacgcag gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg
     3541 gtcagcgtct gactggacac ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg
     3601 taacgttcag ccactttaga tgcggcacgc accatcatac gtttgaggat aacgcccatc
     3661 tgaccgtcgt cgattttctc gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca
     3721 aaacgcacgc ggtgggagct gccaaagcgg ttccacagat aatgcgccac ctgacgcacg
     3781 ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt aatgcacgcg gcagccgcga
     3841 cgcatcaaca tataactgga aacaccggag tcgaaaccac cggaaatgag cgacagcaca
     3901 tcttcctggg tgccaatcgg gaaaccacca ataccttcgt agcgaccttt aatcagcagg
     3961 aggcgatcgt cttccacttc cagatgaaca gttacatccg ggttagtcag cttcacgcgc
     4021 gcggattcaa tatgctgatt taaaccgccg ccgacgtaac gttccacatc aatcgagcta
     4081 aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg ttttgccttc cagctgatcg
     4141 cgatactgaa ccaacgcttt ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg
     4201 acttcgagaa tatggtggat acccggaata cgggtcaggg cgtcgcgaat agtcagacgc
     4261 tggttttcat ctttcgcgcg aacttcgatg ttatcccagt ggcggacgac agcgagcgtc
     4321 tcatcatagt gctttaaaac gttacgaatg ttcccggtaa ggatttttat aaagcgcaag
     4381 cgcacagatt ggcttttgat ggtgatttcc gggaacaatt taatgataaa cttcatggcg
     4441 gcaatggttc gttggcaagc cttaagcgac ttgtataggg aaaaatacag cagcccacac
     4501 ctgcggctgc atccaggcgc ggaagtatac cactaacatc gctttgctgc gcacatcacc
     4561 ttaccattgc gcgttatttg ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat
     4621 attgagagtc agacattcat tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag
     4681 gcgctgagcg agctggaaca gattgtaacc cgtctggaaa gtggcgacct gccgctggaa
     4741 gaggcgctga acgagttcga acgcggcgtg cagctggcac gtcaggggca ggccaaatta
     4801 caacaagccg aacagcgcgt acaaattctg ctgtctgaca atgaagacgc ctctctaacc
     4861 ccttttacac cggacaatga gtaatggact ttccgcagca actcgaagcc tgcgttaagc
     4921 aggccaacca ggcgctgagc cgttttatcg ccccactgcc ctttcagaac actcccgtgg
     4981 tcgaaaccat gcagtatggc gcattattag gtggtaagcg cctgcgacct ttcctggttt
     5041 atgccaccgg tcatatgttt ggcgttagca caaacacgct ggacgcaccc gctgctgccg
     5101 tagagtgtat ccacgcttac tcattaattc atgatgattt accggcgatg gatgatgacg
     5161 atctgcgtcg cggtttgccg acctgccatg tgaagtttgg cgaagcaaac gcgattctcg
     5221 ctggcgacgc tttacaaacg ctggcgttct cgattctaag cgatgccaat atgccggaag
     5281 tgtcggatcg cgacagaatt tcgatgattt ctgaactggc gagcgccagc ggtattgccg
     5341 gaatgtgcgg tggtcaggca ttagatttag acgcggaagg caaacacgta cctctggacg
     5401 cgcttgagcg tattcatcgt cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg
     5461 gtgcattaag cgccggagat aaagggcgtc gtgctctgcc agtactcgac aagtacgcag
     5521 agagcatcgg ccttgccttc caggttcagg atgacatcct ggatgtggta ggagatactg
     5581 caacgttggg aaaacgccag ggtgccgacc agcaacttgg taaaagtacc taccctgcac
     5641 ttctgggtct tgagcaagcc cggaagaaag cccgggatct gatcgacgat gcccgtcagg
     5701 cgctgaaaca actggctgaa cagtcactcg atacctcggc actggaagcg ctagcggact
     5761 acatcatcca gcgtaataaa taaacaataa gtattaatag gcccctgatg agttttgata
     5821 ttgccaaata cccgaccctg gcactggtcg actccaccca ggagttacga ctgttgccga
     5881 aagagagttt accgaaactc tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc
     5941 gttccagcgg gcacttcgcc tccgggctgg gcacggtcga actgaccgtg gcgctgcact
     6001 atgtctacaa caccccattt gaccaattga tttgggatgt gggacatcag gcttatccgc
     6061 ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc
     6121 acccgttccc gtggcgcggc gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa
     6181 cctccatcag tgccggaatt ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc
     6241 gcaccgtctg tgtgattggc gacggcgcga ttaccgctgg catggcgttt gaagcgatga
     6301 atcacgcggg cgatattcgt cctgatatgc tggtggtcct caacgacaat gaaatgtcga
     6361 tttccgaaaa tgttggcgcg cttaacaacc atctggctca gctgctttcc ggtaagcttt
     6421 actcttcact gcgcgaaggc gggaaaaaag ttttctctgg cgtgccgccc attaaagagc
     6481 tgctcaaacg taccgaagaa catattaaag gcatggtggt gcctggcacg ttgtttgaag
     6541 agctgggatt taactacatc ggcccggtgg acggtcacga tgtgctgggg cttatcacca
     6601 cgctaaagaa catgcgcgac ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag
     6661 gtcgtggtta tgaaccggca gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg
     6721 atccctccag cggttgtttg ccgaaaagta gcggcggttt gccgagctat tcaaaaatct
     6781 ttggcgactg gttgtgcgaa acggcagcga aagacaacaa gctgatggcg attactccgg
     6841 cgatgcgtga aggttccggc atggtcgagt tttcacgtaa attcccggat cgttacttcg
     6901 acgtggctat tgccgagcaa cacgcagtga cctttgccgc cggtctggcg attggtggat
     6961 acaaacccat tgtcgcgatc tactccacct tcctgcaacg cgcctatgat caggtgctgc
     7021 atgacgtggc aattcaaaag ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg
     7081 gtgctgacgg tcaaactcac cagggcgctt ttgatctctc ttacctgcgc tgcataccag
     7141 aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg ccagatgctc tataccggct
     7201 atcactataa cgatggcccg tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg
     7261 aactgacgcc gctggaaaaa ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga
     7321 aactggtgat ccttaacttt ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc
     7381 tgaacgctac gctggtcgat atgcgttttg tgaaaccact tgatgaagcg ttaattctgg
     7441 aaatggccgc cagccatgaa gcgctggtca ccgtagaaga aaacgccatt atgggcggcg
     7501 caggtagcgg agtgaacgaa gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca
     7561 ttggcctgcc ggacttcttt attccgcaag gaactcagga agaaatgcgc gccgaactcg
     7621 gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg gttggcataa tccctactcc
     7681 actcctgcta tgcttaagaa attattcatg gcaggagtgg cagcatgcaa tacaacccct
     7741 taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc
     7801 cagatcgcgg taatcacgca tggacactgc cggaagagag cagccgtccc ataattaaac
     7861 gcgcgctgga aggtggcata aatttctttg ataccgccaa tagctattcc gatggtagca
     7921 gcgaagagat cgttggtcgc gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg
     7981 ccaccaaagt gttccatcgc gttggtgatt taccggaagg attatcccgt gcacaaattt
     8041 tgcgctctat caacgacagc ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa
     8101 ttcatcgctg ggattacaac acgccgatcg aagagacgct ggaagccctg aacgacgtgg
     8161 taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat gcacgcttcg cagtttgctc
     8221 aggcgctgga actacaaaaa cagcactgct gggcgcagtt tgtcagtatg caggatcact
     8281 ataatctgat ttatcgcgaa gaagagcgcg agatgctgcc tctgtgttat caggagggcg
     8341 tggcggtgat tccgtggagc ccgctggcgc gggggcgact gacgcgtcca tggggagaaa
     8401 ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa
     8461 atgacgcgca gatcgcagag cggttaacgg gcgtcagtga agaactcggt gcaacacgag
     8521 cacaagttgc gctggcctgg ttgttaagta aaccgggcat tgccgcaccg attatcggaa
     8581 cttcgcggga agaacagctt gatgagctgc tgaacgcggt ggatatcact ttgaagccat
     8641 aacagattgc cgagctggaa acgccgtata aaccgcatgc ggtcgtagga tttaaataag
     8701 agtccaggcc tgatgagacg tgacaagcgt cacatcaggc atcggtgcac aactacgaca
     8761 gaatacccag cggccagtga tgaccgataa agtacaggat gcctgcggaa atcaccccgg
     8821 cgacaatatc gtcgatcatg atccccatgc cgccatgtac attgcgatca aaccagcgga
     8881 tcggccacgg cttccacata tccagaatac ggaaaatcac aaacccggcg gcaacccact
     8941 gccagtcatt ggtcggcagc gccatgagcg tgatccacat accaataaat tcgtcccaga
     9001 caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt ttgatgacaa agatagacgc
     9061 cgatacagat ccccaacatc accaccagcg agtagagctg ccagggtaaa aaggtcatca
     9121 gataccagaa agggatcgcc gccagcgatc ccatcgagcc aggaacgatt gggcttaatc
     9181 cacttccgaa tccgacagca agcagatgcc acggattact catcttcagg cgacttttcg
     9241 cgacatcttt atggcgtgac aaaatggtca tatcctttcc agtcaaacgt gacaggttcg
     9301 ccgtcacgaa taaaacaaag cccttcgata tcggcggtca tttgcccgat acaggtaaac
     9361 ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac ggttcagttc cggcaccgtg
     9421 aaacacaact cgtaatcttc accgccagag agcgcccagc gcagcgcctg ttccggttca
     9481 acatggcgag aaagcgcatc agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag
     9541 tcgctggctt tcacgatatg cccgagatcg gaaatcagac cgtcagagag atcgatagct
     9601 gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac gcggcgatgg acggagatga
     9661 cgtttgatca agtagtcagc atctttagca tcggcaacct gcaaacggtt ttgcaaaatc
     9721 gccagcccgg cggcgctatc gcccggtgta ccggtcacat agatccagtc acccggtttc
     9781 gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt
     9841 gataatggcc cacgcgtggt atcgccgcca atgagttgca tatcgtaata attgagaaga
     9901 tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt ctacgtccgg taaggttaat
     9961 gccagcgtca gccaggccgg atcggccccc atcgctgcca gatcgcttag gttcaccgcc
    10021 agtgctttat aagccagatc agcaggatcg atatcaggga ggaaatggtt gcccgccacc
    10081 agcgtatcag tgctgatcgc cagggtctgt ttctcgggga tattgagaag agcgcaatcg
    10141 tcgccgatgc ccagttcgac atcaagacga gaacttctta cacggtcaaa ataacgggca
    10201 atcagggaga actcgccaca tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc
    10261 ggcgatcatg gaacagtctt ccgtgaatct accggcctgg atattacttt ttgttagggc
    10321 gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc
    10381 cgaacgattt cgccagttcg atcgcttcgt taatggccac tttgtatggc acatcactac
    10441 gtttagacag ttcgtacagc gcaatgcgca gtactgcttt ttctacctgg cccagttctt
    10501 ccagcaggcg ggacaggtag ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca
    10561 ccccggccag cagctcacgg aagtacagga cgtcaacgtc ttttacatcc tgttcagcca
    10621 ggaactggta ttcaacatca gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct
    10681 ggacggcaca ctcacgagcg cggcgacgag cagcaggttt cacggatttc cccttactaa
    10741 tttcaggcct tgatggcttt caatacatta atcatttcaa gcgcggtcag tgcagcttct
    10801 gcacctttgt tgccagcttt ggtgccagca cgttcgatcg cttgttcaat gctttcagtg
    10861 gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg caacatgcgc caggccgttg
    10921 cttgcaccac cagcgacata ttcaaagtgg gcagtgccac cacgaataac cgtacccagc
    10981 gcaatcaccg cgtcgtattt accggtttta gccagtgcac ccgccgccag cggcagctca
    11041 taggcaccag gcacccaaac aacggtaatg ttttcatctt ttacctgacc gatacgtttc
    11101 agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa agttgttgaa acgcgcgatg
    11161 gtgatggcga cgcgagcgtc cggggtagca acgttagctt caataatgtt cattttcttc
    11221 cttcgggttc gagtatggcc ccgcaggggg gcggatttta gcataatatt tcgtgcgctg
    11281 cttccctttc gagccgggag atcatgcacc cactaaatgc aggcaaacat ccgggcctac
    11341 atgacgtatc tctttgaatt taaattgggg ggcgtcggct aatttctcaa gccctggcag
    11401 cgagcataat ccacgggcgt cgctgcctaa tagtttaggt gcgatataga caatcagctc
    11461 atcgactaaa cccgcctgta gcaatacgcc agcgagcgtt ggccccgctt ccacccagat
    11521 gctgttaatc tgctgtttac ccagttgcat catcagtaca accagatcca gatgaccttt
    11581 atgctctgga atcagcaagg tacgcaccgt ttccggccac tcacgagaat cttcctgcgt
    11641 acgcgcgaac caggtttcgc cgggctgctg cacaatgcga tgttccggcg tcacgcgatt
    11701 ttggctatca atcacaatac gtactggctg gcggagattt tgttgcgggt agagcgcctg
    11761 agtttgttca tccagttcag accaacgcac tgttaaggct ggatcatccg ccagcaccgt
    11821 ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc
    11881 ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc gccgtgcggc catcaagcga
    11941 tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc atccgcttga gaaagccttt
    12001 attcaattgc tcggcttcac tcatcatcag cccgtggctg acgtcaatgc cagcctgttg
    12061 cagacggtaa agtccacgcc cagcgacctg cgggttagga tcttgcatcg ctgcgaccac
    12121 gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt ggcgtacggc catgatggct
    12181 acagggttcg agtgtcacat acgccgttgc gcctttggct ttttcacccg ccatacgcaa
    12241 cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg taaccttcac cgacaatttc
    12301 gccatctttg acaatgacgc acccgacatt cgggttgggg tgggtggtaa aacgtcctcg
    12361 ttgcgccagc tttagcgccc gcgccatgta atactcgtcc tgcacggctt agtcctccag
    12421 gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa ctgcggtaga cagaggcaaa
    12481 acggatatag gcgactttat cgagcttttt caattgctcc atcaccagat tgccaatcat
    12541 cttgctcggt acttcgcgct caccggtggc gcgcagctgc gatttaatat gattgattgc
    12601 catttcaacg taatcggaac tcaccggacg tttttccagc gcccgcagca ttccgctacg
    12661 caatttctct tcattaaacg gttcacgcac gtcgttgctt tttacaacac gcggcataac
    12721 cagctccgcc acttcaaagg tggtgaaacg ttcattacac accagacact gccgacggcg
    12781 gcgtacggat gaaccctcgc ccacgagacg agagtcaatt accttagtgt ccacggcgaa
    12841 acagaatggg caatgcatac ggcgtcctga ccaggtggtt aacggaaatc tattttaccc
    12901 tgaactgacg atacaacaaa ggcgcaacgc ttttgagata aaggatcgat gctttcgctg
    12961 cttttaccct ctaccattag gctaattgcg acgagaaagg gacgacctat gaatactaac
    13021 gtttttcgac tgctcctgct gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt
    13081 gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc aggagatgac gcaactcaat
    13141 caagaaacag tcaaaatcac tcagcaaaac aggctgaatg caaaatccag cagtggggtt
    13201 taccttctgc ctggagcgaa aacaccggca agactggaaa gccagatcgg tactttacgt
    13261 atgtcgctgg tgaatattac gcctgataca gatggcacca cccttacact acgtattcag
    13321 ggtgagtcga atgatccctt gcccgcattc agcggaactg ttgaatatgg gcaaattcag
    13381 ggaacaatag acaactttca ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc
    13441 agcgtcctcg cccccagcga tgttgatatt ccgttacaat taaaggggat ttctgtggat
    13501 cagttaggct ttgtacgtat ccacgacatt caacctgtta tgcagtaaac gtatttcggg
    13561 acgattttgt gcgtcccgca acatcttccc cgtcattttg ttactctgct tacatcacct
    13621 ggattgatag taaaagtttg caacaagggc gaaagtcagt acaatccccg cccgaatgtg
    13681 tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact gtgaaacgaa acatattttt
    13741 gtgagcaatg atttttataa taggctcctc tgtatacgaa atatttagaa acgcaatttg
    13801 cgcctttttc actcccgcaa gggattttca aacagtggca tacatatgaa aaaaacatta
    13861 ctggcagccg gtgcggtact ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac
    13921 gacaaaccgc agtatctttc cgactggtgg caccagagcg ttaacgttgt cggaagctat
    13981 cacacccgtt tcggaccgca gatccgcaac gatacctacc ttgagtacga agcattcgct
    14041 aaaaaagact ggttcgactt ctatggttat gcggatgcgc cggtattctt cggcggtaac
    14101 tccgatgcta aaggtatctg gaaccacggt tctccgctgt ttatggaaat cgaaccacgt
    14161 ttctccatcg acaagctgac caatactgac cttagcttcg gtccgttcaa agagtggtac
    14221 ttcgcgaaca actacattta cgacatgggt cgtaataaag atggtcgcca gagcacctgg
    14281 tacatgggtc tgggtaccga tatcgacact ggcctgccga tgagcctgtc catgaacgtc
    14341 tatgcgaaat accagtggca gaactatggc gcagcgaacg aaaacgagtg ggacggttac
    14401 cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt ggggcggtca gctgagctac
    14461 atcggcttca ccaacttcga ctggggttcc gatttagggg atgacagcgg taacgcaatc
    14521 aacggtatta agacccgtac taataactct atcgcttcca gccatattct ggctctgaac
    14581 tacgatcact ggcactactc tgtcgtagct cgttactggc acgacggtgg tcagtggaac
    14641 gacgatgcag aactgaactt cggcaacggc aacttcaacg ttcgctctac cggctggggt
    14701 ggttacctgg tagtaggtta caacttctga ttatgaaaat gccgggattt attcccggca
    14761 tttctgattg ttaacaacgt gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc
    14821 ggcccacaaa agcacgtaaa ttcaatatat tgctgaaata acgtaggcct gataagcgta
    14881 gcgcatcagg ccattttacg ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca
    14941 gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg catcttcccc tgcgataacg
    15001 gtcgtaccat actgatccgc ttcggtatat ttcgaatgct catgatcgtg gcaatagaga
    15061 cacaacaatt cccagttact gccatcttcc gggttattgg tatggtcgtg atcaatgtgg
    15121 tgaacggtaa gttcacgcag gttagaataa acaaactcgc gggaacagcg accgcagacc
    15181 cacggataga tttttaatgc tttttcgcga tagccgcttt ctaaccgtgc atagtttttt
    15241 gggatgatag ccatgaaaag ttacctggat gaaatgtagg gttacgcgca gtttcccaag
    15301 atgcggggat tttcgcaatc tccatacatc aaaaaaaatc ccgatcttct gaccgggatt
    15361 ttcaacatca acgggaactt gattaaggca gaattgacgg ctgatccgcc ccttcttttt
    15421 ccactttctg ctgcaacatg tgttcgcgct tcatacccag tttcagagcc aacgcagatg
    15481 ctacgtagat ggaagatgca gtgccgatgg aaacaccgat aagcatggtc agcgagaagc
    15541 cttccagtac cggaccaccg aagagataca gcatcaggat aaccatcaag gtagtaccgg
    15601 atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg
    15661 taccgcgacg gatcttgcgg aagttttcac gaattcggtc cgataccacg atactgtcgt
    15721 taagcgagta accgataacc gacatcaacg atgccacaat ggtcaggtca atctcgatat
    15781 ggaataacga caaaataccc agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc
    15841 ctgccgccag tcgccactca aagcggaaac ctacgtacac gaggatagac agcagcgctg
    15901 ccatcaacgc catcgcaccg gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact
    15961 caatacgctt cactgctgca ttctgattgg tggattcgtt aatcaccttc agaacttggc
    16021 tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg catacggacc atgatgtcgt
    16081 ggctgctacc aaagttttgc agcattggct cttcaaaacc ggctttttgc aatgcatcac
    16141 gcattacgtc aatttcagcc ggtttttcga gcgtaatttc gataaccgta ccaccggtga
    16201 aatccagccc ccagttaaag ccgcgcacgc ccataataac gatagcagcg attaacagca
    16261 gaccagagat gccgaaagcc cagtagtccc agcgcataaa gtcatagact ttacggccgt
    16321 ggtttagttg ttcaacagta tattcctgtg ccacatcgca ctcctcagat tgacagcttc
    16381 ttgacgcgct tgccgccata tagcaggttt acgatggcac gcgtaccgac aatcgcggta
    16441 aacatcgacg tcgccacacc gataccggta gtaatcgcga accctttaat tgccccggta
    16501 cccactgcgt acaggatgat gactttaatc agcgtggtga tgttcgcatc gaagatagaa
    16561 ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac
    16621 tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga ccgctaccgc aagggttaag
    16681 acgatacccg cgatacctgg catactcagt gttgcgcctg gcaacagcga cataataccg
    16741 acgatcaaga tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa cttcttatag
    16801 aagatgatca tgaacagaat agaaaccagc agaccggcaa ggcaagcctc cagcccctgc
    16861 tcaatgttct gcatacccag ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc
    16921 gcgatcaacg caccggcacg cagcagcagt gacagctgac gggcttcgtt cgggttgttg
    16981 atgccggtga tacggaagct gttacccaga cgagactgga tgttggcgat gttaatcacc
    17041 tcttcctgtt tcaccagaac cgcacgacca tttgcatctt tcttaccgct gtctttgtac
    17101 tccacaaaca gggttgccat cggtttgccg atgttgtcct tggtgaagtt agacataatg
    17161 ttgccaccag cgctatcgag cgagatgtta acctgcggct ggttgtattc gtcctggctg
    17221 gaagtggagt cggtgatatg gtcaccagtc agaattacgc gtttgtacag cacaaccggc
    17281 tgaccttcgc gggtctgttt cacttcagag tcgcccggta cgcgaccgga tgccgcagcg
    17341 gcctgatcaa cattggtgtt taccagacgg aattccagcg ttgccgtcgc gcccagaatc
    17401 tctttcgcac gcgcagtgtc ctgaataccg ggcagttcaa caacgatacg gtcagcaccc
    17461 tgacgctgaa ccaccggctc cgccacgcca agttggttta cacggttacg caggatatta
    17521 atgttctgct gcaccgcata ttcacgcgct tcactcagac gagcatcgct cattaccgca
    17581 cgcagctggt tgctgccctg gctgctaatc accaggtccg gatggcgctt gctcagatac
    17641 gcaatggctt catcacgagc tttagcatcg cggaaagtga tgctcaggcc gtagttgttt
    17701 tctttacgaa cagtggtata cgggatgccc ttttcgcgca ggtcactgcg taggctatcg
    17761 atattttgtt cctggagttt gccaagcgcg gtgtccatat ccacttccat caggaagtga
    17821 acgccgccac gcaggtcaag gccgagcttc atcggctcag catgaatagc tgccagccag
    17881 cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt tgtcacccat aacgcccatt
    17941 aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc
    18001 tcttccagtg ccacagactt agcagttatt ttttcttctt gtaacgtttt ctggacctgg
    18061 atcagcgttt gctcactggc ggcgactccg cgcgcaccag tgatctgaac agccggatcc
    18121 tcaccaaaca ggttgggaag cgcatacagc agaccgatga caatcaccac gatcagcatg
    18181 acgtacttcc acaaaggata acggtttaac acggcaattc ccttagggaa aaattttaat
    18241 tacagcgcct tcatggtgcc tttcggcagg acggcagcta cgaagtcacg tttaataact
    18301 acttcagtgg tgtcattcag cgcgatagca atgtagccgt tttccgctac tttggttacg
    18361 cgaccaacca ggccaccgtt cgtcagaact tcatcacctt tggcaatgga gtccatcagc
    18421 tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga tcatgaaata gaaaatcaga
    18481 ccgaatacca ccagcatcaa aatcaaagac atcgggctac cttgcgccgg tgcacccgtt
    18541 gccgctaccg catcagaaat aaaaaagctc attaaatttc cctcattatt aatattaatc
    18601 aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa aaatcagtta cgaagctctc
    18661 taatttaccc tcttcaatag ccttgcgtaa acccgccatc aaacgctggt agtaacgaag
    18721 gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa
    18781 gtaagcgcgt gaataattgc gacaggtgta gcaatcacac tcaggatcga gtgggccagt
    18841 atcgctctta tatttcgcat tgcggatttt caccacgcca tcggtcacga acaaatgacc
    18901 atttcgggcg ttgcgggttg gcattacgca gtcaaacata tcgataccgc gacgtacgcc
    18961 ttcaagcagg tcttccggtt taccaacgcc catcaggtaa cgcggtttgt ctgccggaat
    19021 ttgcggacac acatgctcca gaatacggtg catatctgct ttcggctcac ccacagccag
    19081 accgccgaca gcgtagccat caaagccgat atctaccaga cctttaacag atatatcacg
    19141 taaatcttcg taaatactgc cctgaattat accaaataac gcatttttgt taccaagact
    19201 gtcaaaacgc tcacggctac gcttcgccca acgcagagac atctccatag agcgttttgc
    19261 gtaatcccag tcagcaggat acggcgtaca ctcatcaaag atcatgacga tatccgaacc
    19321 aagatcgtac tgaatctcca tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat
    19381 cgggttacgg aagtgaacgc cctgttcggt gattttacga atatcaccaa ggctgaagac
    19441 ctggaagccg ccggagtcgg taagaatcgg tcctttccac tgcataaaat cgtgcagatc
    19501 gccgtgcagt ttcatgattt cctggcccgg gcgcagccac aggtggaagg tgttgccgag
    19561 gataatttgc gcgccagtgg cttcaacttc ttccggcgtc atccctttta cggtgccgta
    19621 ggtgccaaca ggcataaaac aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc
    19681 acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc attttttctc cactacgtca
    19741 gaaaaacagt ccaacgttta aaccagcgcc gcggaattac tccccgacgc gctcattaat
    19801 tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc
    19861 ttctaccgcc gctttatagg cattcatggt gtgttgataa ccggcaaagg ccgaaaccag
    19921 cataatcagc gtcgactctg gcaagtggaa gttcgtcacc agcgcatcga ccactttgta
    19981 ctggaagccc ggatagataa agatttgggt atcgtcaaag aacggttcga taagatcgtt
    20041 ttttgccgcc tgtgccgcgc tttccagcga acgtactgaa gtggtgccaa ccgcaatcac
    20101 ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg gtacttcagc
    20161 gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc
    20221 cgcaccaacg tgcaacgtca caaacgccat ctccacgcct ttggcgcgta atttttccag
    20281 caaaggctcg tcaaaatgca gaccagcggt cggggctgca accgcgcccg gtttttcgct
    20341 ataaacggtt tgataaagtt cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg
    20401 cagcggcata tggccgatgc tgttgaggat atccagtacc gagcgttcat cattaaattc
    20461 gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc
    20521 cagcaacagt tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg
    20581 tttgtcgtcg agcatccgct caaccagcac ttcaatcttg ccgccgctgg ctttacgccc
    20641 aaacagacgc gccgggatca cgcgggtatt attaaaaacc agaagatcgc cggggttgag
    20701 cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga
    20761 cagtaaacga cagctactgc gttcaggcat gggatagtgg gcaatcaggg attcgggcaa
    20821 ttcaaaggag aaatcggtaa cgcgcatgac actgactcag actaaaataa gagtcgggta
    20881 gtctagtgcc ggggcgctct ccctgcaacc tttacccccc tttggataga atataatcga
    20941 tgaatttttt agctcacctg catttagccc atctcgcgga aagctcgctt tctggcaatt
    21001 tactggctga tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg
    21061 gcattcatat gcatcgacgt attgactgac aatctgccgg aagtccgcga agcacaggag
    21121 tggtttcgta gtgaaacgcg ccgcgttgcg cctattacgc tggatgtcat gtgggatcac
    21181 tttctttccc gccactggtc gcagctgtcg ccggactttc cgctacagga atttgtctgt
    21241 tatgcccgcg agcaagtgat gacgattttg ccggactcac cgccacgttt tatcaatctg
    21301 aacaattact tgtggtcaga gcagtggctg gtgcgctatc gcgatatgga tttcatccag
    21361 aacgtgttaa acggcatggc aagccgccgc ccacgtctgg atgccctgcg tgactcctgg
    21421 tacgatttag acgctcatta tgacgccctc gaaacccgct tctggcagtt ttatccgcgg
    21481 atgatggcgc aggcgtcaca caaggcgtta taaaacgtta gttcatccat accgtagccg
    21541 aaatggcagg caaagccaga atcccgtcag tcagttgccc atgcccttct ttgcattgcc
    21601 attgcacggc attgagaaac ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt
    21661 tgattgccac cagtacacgt tgctgattca gcacgcggac aaataccact acgttatctt
    21721 ccgcatacag cacctgacag ccgccacgac gcagcgcctg acttttctta cgcagcacaa
    21781 tcattcgccg gtacagcgcg aataacgccg tatcctgctt ttccacctgc caggggaacg
    21841 gtttacggca aaacggatcg tttttaccat ccagtcccac ttcatcaccg taataaatgc
    21901 acggtacacc aggccaggtg aacagccaga ccaccgccag cggcaggcgc gcaatatccc
    21961 gaccgagcag cgttttaaat cgcgcagtat cgtggctatc gagctgatta aacatacgta
    22021 attgttgttg atgagaaagc cctgcgcggt aattatccat ccaggccata caggtttggg
    22081 cgtcaatttg ctgcggatca taagagatgt cggtattggc aagaaatccc cacaacggga
    22141 atgtgaagcc acgatagttc atggcagcat cttccacatc ggcctgtaac cattgccgtg
    22201 catcgccaaa atgttcgccg acaatatacg cttccggctg ggtttctttc gccgcttcgg
    22261 tgatcccggc aacgtgctgc atattattgc gcgccccacc cgcctccccc agcatatgca
    22321 ccacatccag ccgccagccg tccatattcc acggcgcttt cagccagtgg cggacaatac
    22381 tgtcttcccc gcgataaatt tcattcacca gactttccga ctgataatcc agcttcgtca
    22441 agctggcata gccaagccag tcgagcgccg tgccatcatc actaaacgag taccagtcgc
    22501 gccagggcga ttcagagttg tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc
    22561 aggcatggga atcgccactg tggttaaaca cgccgtccag caccagccgc attcccagct
    22621 gctgcgtatt gtgtcgcaaa cgcagcaacg ccccatcacc gccaaactgc ggatcgacat
    22681 ggcgataatc ctcggtatcg tatttatgta cgctgggagc tttaaacacc ggattgagat
    22741 acagcgctgt cacgccaagc tttttcagat acggcagttt ttcgcttatc ccgtccagat
    22801 cgccgccata gaacgttgat ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca
    22861 agatgatctc ttgtccggct gcatgatggt aatagacatg atcctgttca gcttcacgag
    22921 gaagactacg cgcaaaacga tcagggaaga tctgataaaa aatctgatcc gcagcccatt
    22981 gtgggccgat atcaggtaca tcgacggcaa actgctccag tcgtgccggc ggcattcggc
    23041 tgaagccctg cggtgtaaac caacgctggc gatcgtgcca cagcagtttg aaactgtaac
    23101 gccgccgggg ttgtccgctg gagagatcaa tcgccgcacg ccatgcggtg acgcctggct
    23161 gcggctgact gcgctgctta tgcattggta ctgacatttc ttcgttatcg tgttctgtac
    23221 gcagcataat gcgctgcggt gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat
    23281 ctttgctttg tttaacaaat gggggcaccg gcaggtgcca tgcatttaac atcataattc
    23341 ccctgtgatg aaaatctgat caccttgcca tagcctaatg caggatcact catcatctga
    23401 aggatatttg ggggaggagg acgggggcgg ataactttcg gaatagccgg atcaggattt
    23461 cctcctgaac cggcttacag aacgcaagta ttattggttt tcagccagct gacgatcgtg
    23521 gcggcgttta aacatccagc caatcaacag cacaacaatc cacgcgaaac cgacatacag
    23581 cgagatacgc gtatccgggt gataaccaat caacccgata ataaagagca ggaaaatcag
    23641 cccgccgatg gtcgttgcta ccccacccgg cactttaaat ttcagcgcct taacttcttc
    23701 tggcggcaaa cggcgacgga aggcgatttg cgacagcagg atcataatcc acacccatac
    23761 cgtggcgaag gttgccagcg aagcgatcac caggaagacg ttttccggca tgatgtagtt
    23821 cagatacacc gcaaacagca gcgcggtagt catcaccagc accgtaaccc acggaatacc
    23881 gcgacgcgac gttttgctga aaattttcgg tgcgctgccc tgctctgcca taccgtggag
    23941 catacggcct acgccaaata cgtcactgtt aattgccgac agcgaagcgg tcagcacaac
    24001 aaagttaaga atgctggcgg caaaggtaat gcccatatgc tggaacgtca gtacgaacgg
    24061 gctaccggca gtgccaacct gattccacgg gtagatagac ataatgacga acagcgtacc
    24121 aacgtagaac accagaatac gcatcggcac ggagttaatc gcacgcggaa tcgatttctc
    24181 aggatctttc gcttcaccgg cggtaatccc gataatttcg atcccaccgt aagcaaacat
    24241 caccatttgc aacgacatta ccatgccaag ccagccgtta ctgaagaagc cgccgttgct
    24301 ccacaggtta tgaataccgg tcggttgccc gccgttgcca atcccccaga tgatgatgcc
    24361 gaaaccggcg acaatcatga tgatgatggt ggcgacttta aagaacgaga accagaattc
    24421 cagctcaccg aagaccttca cgctcatcag gtttacggcg caaatgatca gcaccacgct
    24481 cagtacccaa atccagtgcg gcaccgtcgg gaacaagaca cccatataga taccaaaagc
    24541 ggtcacatcg gcgatggcga caataaggat ttcaaaacag taggtccatc cggtaatgta
    24601 acctgccagc gggccaaggt tttcctgcgc ataacgcgag aaagagctgg cggccgggtt
    24661 atgtaccgac atttccccca gcgcacgcat aatgatatac gccgcgatac caccgataat
    24721 ataggccaac aacacgctcg gaccggccat tttgatggcg tctgccgaac cgtaaaacag
    24781 cccggtgcca attgctgaac ccagtgccat aaagcgtatg tggcgggtac ttagcccacg
    24841 ctttagctta ttcttacttt ccatcaattc ctaacccatc aacacaataa aaaaccacgg
    24901 ggcatcgcag ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac
    24961 ctgacgacct gccgcgcgat cccagataat ggccagaacc accatcacca ctgttggcat
    25021 taaccacgcc agaccttgtt cggccagcgg taaacgctgc gcccaggacg gtaagatatc
    25081 gctgaatgca gatgctttga tcccgtcgag aataccaaaa agcaggctga taaacatcgg
    25141 cggagcaatc acgcgggacg aattatgcca ccatgagcgt gtaaaactta atacaaccag
    25201 tgcgatacac ggcggataaa tagcggtcag caccggtacg gagatctgga tcagctggct
    25261 taagccgagg ttagacacca ccatcgagaa gccgccgagg ataaacacca gcgtacgata
    25321 agagagcggt acgtactggg caaagaattc tgcacaagca caggtcaggc caaccgccgt
    25381 gaccagacag gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt
    25441 gtgctgaacg taagcatgca gaatagcagc gccgtttgca gactgatcga ccagcgacgc
    25501 gctgtctgaa cccagacaga acagcgccag gtacagcaaa gtcagaccaa cacccgccat
    25561 caggccagcc cagacggtat aacgggtcag cagacgcgct tcggtaacgc cacgagaacg
    25621 cgccgcgtta acaataacga taccaaacac cattgcgccc agcgtatcca tggtcagata
    25681 gccgttaacg aagccgttag aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat
    25741 agaacccgcc ggccagacaa tcgcgacaac agacaggatg accagcgcga taattttcag
    25801 cggcgcaagg aagttgccca cggtatccag cagcttgccc ggatagagcg aaaccagaat
    25861 aacgatagcg aaatagacca ggctgtagat aaacagcggc agcgcggaat cacccgtcag
    25921 cggcgcaatc cccacttcaa aggaaacggt agctgtacgc ggcgtagcga aaagcggccc
    25981 caccgccagg taacaaaccg ttgccagcag tacgccagcg actttaccga ttggcgtgct
    26041 gaggctgtca acaccgccgc caacttttgc cagcgccact accgttaata ccggcaggcc
    26101 aacggcagta atgaggaagc cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc
    26161 gaccattgga gggaaaataa tgttacctgc gccgacgaac aacgcaaatg tcataaagcc
    26221 cagagcgatg atatcgcgcg atcttaattg atgggtcata aatcatactg cctgtggatg
    26281 tggtgttgta acgttaaatt tttgtgccaa tccctgggga tgatacagcg gtatatttaa
    26341 acaattttag cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc
    26401 gcgttaatgc agtcagtatg acagcacctg aagcgcaatt taaacgctta taacgtttta
    26461 aagcaaggag ggatctaaaa accagaggta tatagcgata agaaatcacc aaccagtata
    26521 aaacactatt tccgtgaaaa agacatggct aatcatgcga acaaaaaatc accaaccgtt
    26581 gtaaaatcta acgcccgtat tttgactgca aagaaaataa gccagcatcg gctggcttat
    26641 ggcaataaaa gatgacaaag gcggattaat cgctgttttt ggcaattaaa cgttccggga
    26701 taacaaaact gaaacgtgtt ccttttccta ctgtactctc aatattcagg cgactttcgt
    26761 gatgattcac cgcatgtttc acgatcgcta accctaatcc gctaccgccg gtttgccggg
    26821 aacgcgcttt atcaacgcga taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa
    26881 tgcccggtcc gttatcttca acgctaaatt cggcaccgtg cggcactcgc tgccagcgta
    26941 cggtgatatg cgtgccttcc ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg
    27001 cactgcgtag ctgatcttcg ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa
    27061 atgtctgttt tttctgactc agagtctgag cctcgcgctc aacaacgcgc agcatcatcg
    27121 gcacatcaac cttttcattg agcaattgcg tcggcgcggc ttcaattttc gacagcgtta
    27181 gcaactgctt caccagccct tccatccgct gggtctgctc gcgcatggtg tgcaacgctt
    27241 tttcgcgtac cgcgccttcc agcggctgct catccatcat ctccaggtaa ccctgtaaca
    27301 cggtcaatgg cgtacgtaac tcatggctca cgttggcaaa aaagttacgc cgcgcccctt
    27361 ccagttgatg catttgcgtg acatcacgcg ccaccatcag caactgtttg tgggtataag
    27421 gcatgacgcg aatttccaga tgccgcccgg tgttgagcac cagattgagc gggcgagaaa
    27481 aatcacgcgt tttcagatat tgcgtaaact ccgggtaacg cagtaggtta aggatgttct
    27541 gcccgttatc ttccggccag cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga
    27601 aaataccgcc ctcttccgtg gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac
    27661 gaaagcgttt aatcagattg cccagttcac ggcggcgttt tttatttcgc agctgcatct
    27721 ggtgtaagcc gtatagtagc ggttcccagc taccacgccc cggtggcggg gtcatactgc
    27781 gatccaccca cagccaccat gaaaggcgca ataaattcca gaaatgccag ataagcagtc
    27841 ctgttaccga tgccagcaaa aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag
    27901 ccgggaggca gcaaagtagc agctccagca ccagcctttt ccacgacagc cgttccagca
    27961 cgtaagatac tccagttaag aaatcataag ccctgctctg cgtccgatga gcaaggcgtt
    28021 aaaagcgggt tgaaaaacga tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc
    28081 cgccgggctc cagtgcttta cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca
    28141 catacacgtt agttccccag acgtggttta acagctgctc gcggctgtac acgcgctcag
    28201 gatgcgtcat aaaaaagtgc agcagtttaa attctgtcgg ccccatctcc agcggctctt
    28261 cgcccgccat cactcggtga gatgtcgggt tgagacttaa tccctgcatc ttaatcacct
    28321 cttccaccgc cattggcgaa atacggcgca ttaccgcttt gattcgcgcc accagctcct
    28381 tcggcgaaaa tggcttggtg atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat
    28441 cttcttcttc ccctctggcg gtcaacatca ccactggaat atcccgggtc atcgactcgc
    28501 gcttgaggtg tttgatgaac tggataccgg agccgccagg taacatccag tcaaggagaa
    28561 ttaaatccgg ccagggttca ttcagttgat tcacagcact gtcataatct tccgcttcga
    28621 ccggctgaaa gccattttgt tcgagcacga agcagaccat ttcgcgaatt ggagcttcat
    28681 cttctacgac cagaatacgt ctcgccatga tttgccctgt tgtaataaat aggttgcgat
    28741 cattaatgcg acgtcattat gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac
    28801 atatcttcag gttattgatt tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg
    28861 tataatcgcg accatgcttt ttcgccaggg aaccgttatg cgcatccttc acacctcaga
    28921 ctggcatctc ggccagaact tctacagtaa aagccgcgaa gctgaacatc aggcttttct
    28981 tgactggctg ctggagacag cacaaaccca tcaggtggat gcgattattg ttgctggtga
    29041 tgttttcgat accggctcgc cgcccagtta cgcccgcacg ttatacaacc gttttgttgt
    29101 caatttacag caaactggct gtcatctggt ggtactggca ggaaaccatg actcggtcgc
    29161 cacgctgaat gaatcgcgcg atatcatggc gttcctcaat acaaccgtgg tcgccagcgc
    29221 cggacatgcg ccgcaaatct tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc
    29281 cattccgttt ttacgcccgc gtgacattat ttccagccag gcggggctta acggtattga
    29341 aaaacagcag catttactgg cagcgattac cgattattac caacaacact atgccgatgc
    29401 ctgcaaactg cgcggcgatc agcctctgcc catcatcgcc acgggacatt taacgaccgt
    29461 gggttccagt aaaagtgacg ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc
    29521 ggcacaaaac tttccaccag ccgactacat cgcgctcggg catattcacc gcgcacagat
    29581 tattggcggc atggaacatg ttcgctattg cggttccccc attccactga gttttgatga
    29641 atgcggtaag agtaaatatg tccatctggt gacattttca aacggcaaat tagagagcgt
    29701 ggaaaacctg aacgtaccgg taactcaacc catggcggtg ctgaaaggcg atctggcgtc
    29761 gattaccgca cagctggaac agtggcgcga tgtatcgcag gagccacctg tctggctgga
    29821 tatcgaaatc actactgatg agtatctgca tgatattcag cgcaaaatcc aggcattaac
    29881 cgaatcattg cctgtcgaag tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt
    29941 gttagccagc caacagcgtg aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg
    30001 ccgtctggca ctggaagacc tggatgaatc gcagcagcaa cgtctgcagc atcttttcac
    30061 cacgacgttg cgtaccctcg ccggagaaca cgaagcatga aaattctcag cctgcgcctg
    30121 aaaaacctga actcattaaa aggcgaatgg aagattgatt tcacccgcga gccgttcgcc
    30181 agcaacgggc tgtttgccat taccggccca accggtgcgg ggaaaaccac cctgctggac
    30241 gccatttgtc tggcgctgta tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa
    30301 aatgatctca tgacccgcga taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa
    30361 ggtgaagcgt atcgcgcgtt ctggagccag aatcgggcgc gtaaccagcc ggacggtaat
    30421 ttgcaggtgc ctcgcgtaga gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa
    30481 gtgaaagata agctggaact gacagcgacg ttaaccgggc tggattacgg gcgcttcacc
    30541 cgttcgatgc tgctttcgca ggggcaattt gctgccttcc tgaatgccaa acccaaagaa
    30601 cgcgcggaat tgctcgagga gttaaccggc actgaaatct acgggcaaat ctcggcgatg
    30661 gtttttgagc agcacaaatc ggcccgcact gagctggaga agctgcaagc gcaggccagc
    30721 ggcgtcacgt tgctcacgcc ggaacaagtg caatcgctga cagcgagttt gcaggtactt
    30781 actgacgaag aaaaacagtt aattaccgcg cagcagcaag aacaacaatc gctaaactgg
    30841 ttaacgcgtc aggacgaatt gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag
    30901 gcgttagccg aagaagaaaa agcgcaacct caactggcgg cgcttagtct ggcacaaccg
    30961 gcacgaaatc ttcgtccaca ctgggaacgc atcgcagaac acagcgcggc gctggcgcat
    31021 attcgccagc agattgaaga agtaaatact cgcttacaga gcacaatggc gcttcgcgcg
    31081 agcattcgcc accacgcggc gaagcagtca gcagaattac agcagcagca acaaagcctg
    31141 aatacctggt tacaggaaca cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg
    31201 cgtgcgcagt tctcccaaca aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag
    31261 ttaacccatg ctgagcaaaa acttaatgcg cttgcggcga tcacgttgac gttaaccgcc
    31321 gatgaagttg ctaccgccct ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg
    31381 gtcgcgctgc atgggcagat tgttccccaa caaaaacgtc tggcgcagtt aatggtcact
    31441 atccagaatg tcactctgga acagacgcaa cgtaatgtcg cacttaacga aatgcgccag
    31501 cgttataaag aaaagacgca gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg
    31561 cgcatcaaaa cgctggaagc ccagcgtgca cagttacagg cgggtcagcc ttgtccactt
    31621 tgtggttcca ccagccaccc ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat
    31681 cagtctcgat tactggcgct ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg
    31741 ctgcgcgggc aactggatgc attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa
    31801 agcctccgac aagatgagca agcacttact caacaatggc aagccgtcac ggccagcctc
    31861 aatatcacct tgcagccaca ggacgatatt caaccgtggc tggatgcaca agatgagcac
    31921 gaacgccagc tgcggttact cagccaacga catgaattac aagggcagat tgccgcgcat
    31981 aatcagcaaa ttatccagta tcaacagcaa attgaacaac gtcagcagca gctcttaacg
    32041 gcattggcgg gttatgcact gacattgcca caggaagatg aagaagagag ctggttggcg
    32101 acacgtcagc aagaagcgca gagctggcag caacgccaga acgaattaac cgcgctgcaa
    32161 aaccgtattc agcagctgac accgattctg gaaacgttgc cgcaaagtga tgatctcccg
    32221 cacagcgaag aaactgtggc gctggataac tggcggcagg ttcatgaaca atgtctcgca
    32281 ttacacagcc agcagcagac gttacagcaa caggatgttc tggcggcgca aagtctgcaa
    32341 aaagcccaag cgcagtttga caccgcgcta caggccagcg tctttgacga tcagcaggcg
    32401 ttccttgcgg cgctaatgga tgaacaaaca ctaacgcagc tggaacagct caagcagaat
    32461 ctggaaaacc agcgccgtca ggcgcaaacg ctggtcactc agacagcaga aacgctggca
    32521 cagcatcaac aacaccgacc tgacgggttg gctctcactg tgacggtgga gcagattcag
    32581 caagagttag cgcaaactca ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt
    32641 cgccagcagc tgaagcagga tgcagataac cgtcagcaac aacaaacctt aatgcagcaa
    32701 attgctcaaa tgacgcagca ggttgaggac tggggatatc tgaattcgct aataggttcc
    32761 aaagagggcg ataaattccg caagtttgcc caggggctga cgctggataa tttagtccat
    32821 ctcgctaatc agcaacttac ccggctgcac gtgcgctatc tgttacagcg caaagccagc
    32881 gaggcgctgg aagtcgaggt tgttgatacc tggcaggcag atgcggtacg cgatacccgt
    32941 accctttccg gcggcgaaag tttcctcgtt agtctggcgc tggcgctggc gctttcggat
    33001 ctggtcagcc ataaaacacg tattgactcg ctgttccttg atgaaggttt tggcacgctg
    33061 gatagcgaaa cgctggatac cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa
    33121 accatcggtg tgattagcca cgtagaagcg atgaaagagc gtattccggt gcagatcaaa
    33181 gtgaaaaaga tcaacggcct gggctacagc aaactggaaa gtacgtttgc agtgaaataa
    33241 ctattcagca ggataatgaa tacagagggg tgaattatct cttggccttg ctggtcgtta
    33301 tcctgcaagc tatcacttta ttggctacgg tgattggtag ccgttctggt ggttgtgatg
    33361 gtggtatgaa aaaagtcatt ttatctttgg ctctgggcac gtttggtttg gggatggccg
    33421 aatttggcat tatgagcgtg ctcacggagc tggcgcataa cgtaggaatt tcgattcctg
    33481 ctgccgggca tatgatctcg tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg
    33541 cactcttttc cagccgctac tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg
    33601 tcattggcaa cgccatgttc acgctctctt cgtcttacct gatgctcgcc attggtcggc
    33661 tggtatccgg ctttccgcat ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa
    33721 ttatcaaacc cggaaaagtc accgccgccg tggcggggat ggtttccggg atgacagtcg
    33781 ccaatttgct gggcattccg ctgggaacgt atttaagtca ggaatgctgg cgttacacct
    33841 ttttattgat cgctgttttt aatattgcgg tgatggcatc ggtctatttt tgggtgccag
    33901 atattcgcga cgaggcgaaa ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg
    33961 ccccgtggtt aattttcgcc gccacgatgt ttggcaacgc aggtgtgttt gcctggttca
    34021 gctacgtaaa gccatacatg atgtttattt ccggtttttc ggaaacggcg atgaccttta
    34081 ttatgatgtt agttgggcta gggatggtgc tgggaaatat gctaagtggc aggatttcag
    34141 gacgttattc accactgcgc attgcagcag tgactgactt tatcattgta ctggcactgc
    34201 tgatgctctt tttctgcggc ggcatgaaaa caacgtcgct tatttttgct tttatttgtt
    34261 gcgcgggatt atttgccctt tcagcacctc tgcaaatatt gttactgcaa aatgccaaag
    34321 gaggagagtt attaggtgcc gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg
    34381 gcgcatattg cggaggtatg atgctgacgc tggggctggc atataattac gtggcgctgc
    34441 ctgccgccct gctttcgttt gctgcgatgt catcgttgct gctgtatggt cgctataagc
    34501 gccagcaagc ggcggatagt ccggtgctgg cgaaaccact ggggtagatt actgtcacgg
    34561 tgcgcgttgc ttattgccgg atgcggcgtg aacgccttat ccgccctacg cggttctggc
    34621 acattttgta ggcctgataa gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc
    34681 ctacgttttt tactcttgtg gccataacca cgcagcgccg cgtacgccgc tggaatcacc
    34741 gtgcttcgcc ttactcaccg gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa
    34801 ctgcccaacc gtttgatata aacggtctac attgctcatc ccgcccccca ggacaatcac
    34861 atccggatcg agaatattca cgacatgtgc cagcgatttt gccagccgca gctcgtagcg
    34921 acgcaatgcc agttccgcta ccggatcgct ttcttcaacc aggcggataa tttcactgcc
    34981 tttcagcgca tgtccgctca aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa
    35041 ggtttcaata caaccttgtt taccgcaata acaagggact tcctcgcgat aacgcagttc
    35101 gtcttcgtcc atccacggta gcggattgtg tccccactca cctgccgtgc cattgccgcc
    35161 gatatgcgtc cgcccattga atgccacgcc cgcgccgcat cccgtgccga taatcacggc
    35221 aaataccgtc tgcgctcccg ctgccgcgcc atctactgct tctgaaaccg ccagacagtt
    35281 ggcatcattt gccagccgca cttcccgctg caacctcgcg cttaagtctt tatcgaatgg
    35341 ctgaccgttg agccaggttg aattgacatt cttcaccaca ccggtgtagg gcgaaatcga
    35401 acctggaatg cccataccta ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac
    35461 caacgtggcg atcgtttcaa tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg
    35521 gcggtacaac tgctcccctg catcgcccag tgcaatcact tcagttttgg tgccgcctaa
    35581 atcgatacct atacgcactg tactctcctt atttttttca atatcaatag cgtagagacg
    35641 gacaaccgga ttggcaatgc aaggccgccg acaattcgtt atcatgcccg ctaaatttaa
    35701 cgacaaggcc gtggaaatta tcatgctgtg gttcaaaaat ttaatggttt accgtcttag
    35761 ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag ctagcctcga tggcatttac
    35821 cccatgcggc agccaggaca tggcgaagat gggctgggtt cctccgatgg gatcgcacag
    35881 cgatgcgtta acgcacgttg ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa
    35941 aatcctcccg tctccggtga ttaaacaggc gctggaagcg aaaatcgcca aactggaagc
    36001 ggaacaggcg cgtaagctga agaaaaccga aaaagattcg ctgaaagacg aagtgctgca
    36061 ctctctgctg ccgcgtgctt tcagccgttt tagccagaca atgatgtggc tcgacacggt
    36121 taacggtttg attatggtgg actgcgccag tgccaaaaaa gcggaagata cgctggcatt
    36181 actgcgtaaa agcctggggt cgttaccggt tgtaccattg agcatggaaa acccgattga
    36241 actgacgctg accgaatggg ttcgctccgg tagtgcggca cagggcttcc agctgcttga
    36301 tgaagccgag ctgaaatcgt tgctggaaga tggcggcgtg atccgcgcga agaaacaaga
    36361 tctgaccagc gaagagatca ccaatcacat tgaagccgga aaagtggtga ctaaactggc
    36421 gctcgactgg cagcagcgca ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct
    36481 taagttctgc gacgagctgc gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca
    36541 gcgttttgat gccgatttca tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct
    36601 gattgaagga ttaggtggcg aagcacaacg ttaattgctg attttccttt aatgccggat
    36661 gcgacgcctg ccgcgtctta tccggcgtac gaagccacac caggcatata attattcgct
    36721 acggcgagca ataattttta gcgcagcaat attatgcgtt ttacgccgta actgatctaa
    36781 tcgcctcatc cctattcgcc tgtcagcagc acgcagaata ttcactaacc cccatttatc
    36841 gctggtaaat tgttttgtta acagctccgc ttttggggta tcaatatatt cacgcaataa
    36901 atcagaaatt gaacaaatat ccgtcaggta aggataccag caagtttcac cgtgaaaatt
    36961 acgcactcca gcgttacctg caataaagtc tttcgggtaa tcccagatca cgtttttatc
    37021 caacgtgatc caataacgcg gtaaatcggt acttccacca ttttggctgc gcatgggata
    37081 acgggtacag tgaatttgca ggttgatcgt tggcgtcagt aagtcataga tctcccgctg
    37141 taacttgctc catggacgtt gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg
    37201 cgaattttcc gcgttgcccc ggttttcact aaatcacgta actgcgaaag atcgggttgt
    37261 tcgacaaata agagtaatga tggatcctct tttaaccacg ccgttttcac atcggcagcc
    37321 agctgtggat tattaatagc cagcgaccga tcctgaggct cagtcaatgt cgttaacaac
    37381 gacgccgggg tatgtgcgtt gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc
    37441 agcaatgcac gccaccattc atcatctgat ttatctttta tcgtagccag ttccgcaaaa
    37501 aatacctgga gatcttcact gatatttata tcaaccttga tgaattatga atatatcgga
    37561 aattgaaaaa atcgcagcaa aggaaatatt attatgggtt aaagagagga tatttcagaa
    37621 aaatgccctc ccgttcaagg ggaaggcgag gaattacaga tagcggcaca gataagaggt
    37681 aggttcggca acttgaagat gaaactcact gtgaccggga acattaaaca ccgaaccggc
    37741 ttcatacact tgccagtcgg tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac
    37801 cgtcatctct tccggctcag cggtgctgaa ggtgtattcg ccttcaacca taacacccac
    37861 gctggcgcga ccagtgctgc tgctggaaaa gccgatcgat ttcactttgc cggaaaagta
    37921 ctcattactt tgaagcataa actggccctg tctggtagtt gaacatctac ttcactatag
    37981 gggccagagg cgctggctgt cacgcaaaat tacaccagta attccgcagc cagccgtgca
    38041 atcaatacgt tagagagcaa gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga
    38101 cgttggtgaa atcctaaaca atccagcatg atgacatcag ccccttttgc cagtaattct
    38161 ttcccagcat caatgatttt ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc
    38221 ggcgatttct gcaaaatttg ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga
    38281 acgataaccc ccacctgatg atcttcaaca atagaggaaa ccagtggagg caatattcgc
    38341 gacggctcaa gaaagatcgt attacgcgca gtcatactac taatgtttgc tgtactcatt
    38401 aataaaatga cgtcataacc ctgattatcg agcacttcaa ccacaccttg cagatctcgc
    38461 tccactttac gacgcgaaac atgggccagc tggttgtcat ttaataatgt gagaatggtg
    38521 tcttcgcctg cttctggcgc gtactccgcc atcacttctt cacgacttaa cttccccagc
    38581 aggctatgat gggaaatatt atcttcgtca atgtattccg tcaggagcgg caaaacctcc
    38641 tgcataggta caatgccgat ggtcaggatc gccaacgacg cactcattgt tcatccacct
    38701 tacttttctt tcgtcgttaa ttaccgggca agtgtgaagc accactgctg acacttactt
    38761 gtgctacaaa tgacaaaaag cgtagcagca gacgtgcacg gcataatgta aagattccaa
    38821 atgattccag taatggaatg ttattgttta atattctaat tattagaaaa acataaatta
    38881 tgaaaaatgt gacgcagatc ccttgtagtg gcactcagaa tcccttccga gcagtctgat
    38941 gcggtcactc tttgtcaggg tcctcatagc gttttttcgc atccatcgct tcctgagccg
    39001 tcggatgttc actgatcaac gagtctggtt taggatgatc ggctctgagt tggtaccagg
    39061 ttaccgtctg tcctggcttt cctttttcaa tcgtcactat atatgcttca cgaggataag
    39121 gcggtttcgt tggcatcgtt cttccttatt tcacgggatg aacgttaagt ataggcgctc
    39181 taaaatcaac aattgatcgt ctgtgccagg gcgctacgaa tttcagaaat cacctggctg
    39241 ggttcgtttg ttgcgtcgat gataatatgc gcaacttcgc gatatagcgc atcgcgttct
    39301 tccagcactt cctgaacttc ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct
    39361 tcttccggtg cagcttgcag tcggttaacc aggactgata ctggcgcaca caaataaacc
    39421 acgatcccgt tattttgcat gaagtgacga ttaaattccg tcagaataat gccgccgcct
    39481 gtagcgataa cggtggatgc cgcagttacc gcttccagcg ccgccgtttc tctggcgcga
    39541 aatcccgccc actcttccct ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc
    39601 aaccactgat cggtatcgac aaaccgacgg ttaagcgaat cggcaagggc cattccgacc
    39661 gttgttttac cacagccccg aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt
    39721 ccccaatagg tcgcaatgcg gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa
    39781 actagtacaa tttcgattgt aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc
    39841 ccttactata ccaataaata ttcaagaatg aagtgtaaat aataaattac atttagccac
    39901 gactacgttg cacttccagc caccacttct ccagctccgc ggcaaaggcc tggcggtcac
    39961 gttgtgaaag gctatctggt ccgccggtct ggatcccact ggcacgtaag gtatccataa
    40021 aatcgcgcat cgtcaggcgc tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat
    40081 taagcgccgc tgcgcctttc tcgatggctt cagcagccaa aggtatatct gcggtgatca
    40141 ccaaatcgcc cgcttcacac tgccggacaa tttcgttatc ggcaacatcg aaacctgccg
    40201 cgacgcgcag cgtacgaata aatcgcgatg gcggcacgcg taaactctgg tttgctacca
    40261 gtaccagcgg catctgcata cgttccgccg cgcgatacaa aatctcttta attacattgg
    40321 gacacgcgtc ggcatccacc caaattgtca taaagtcatc ctttgttagg taatcctcta
    40381 ttgtgtcgcg cttttgcctt ccggcatagt tctgtttatg cttctgccag cgattatcaa
    40441 aacaatgaat ttcacggcag gagtgaggca atggaaaaga aaatcggttt tattggctgc
    40501 ggcaatatgg gaaaagccat tctcggcggt ctgattgcca gcggtcaggt gcttccaggg
    40561 caaatctggg tatacacccc ctccccggat aaagtcgccg ccctgcatga ccagttcggc
    40621 atcaacgccg cagaatcggc gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc
    40681 gttaaacctg gcatcatgat taaagtgctt agcgaaatca cctccagcct gaataaagac
    40741 tctctggtcg tttctattgc tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc
    40801 catgaccgga aaattatccg cgccatgccg aacactcccg cgctggttaa tgccgggatg
    40861 acctccgtaa cgccaaacgc gctggtaacc ccagaagata ccgctgatgt gctgaatatt
    40921 ttccgctgct ttggcgaagc ggaagtaatt gctgagccga tgatccaccc ggtggtcggt
    40981 gtgagcggtt cttcgccagc ctacgtattt atgtttatcg aagcgatggc cgacgccgcc
    41041 gtgctgggcg ggatgccacg cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt
    41101 tccgcaaaaa tggtgctgga aacgggagaa catccggggg cactgaaaga tatggtctgc
    41161 tcaccgggag gcaccaccat tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc
    41221 gcagtgatcg aagcgatgac gaagtgtatg gaaaaatcag aaaaactcag caaatcctga
    41281 tgactttcgc cggacgtcag gccgccactt cggtgcggtt acgtccggct ttctttgctt
    41341 tgtaaagcgc caaatctgcc gatttcaacc actcacgata gtgactcatt tgtgggttca
    41401 gcggcgcaac ccccacacta atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg
    41461 tgtttagccc ttcacgcacc cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg
    41521 acatgattac tgcaaactca tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg
    41581 taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca tgaccccagg
    41641 tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca tcgcgattat
    41701 gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt cggttataca
    41761 cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt tccgccagtt
    41821 tggttgccgt ctggtagctg acccagccaa acatcagagg ataaacgaca ataatgggaa
    41881 gggagagcca ccattccagc ggagcactat tgaacgacac ggtaatgccc gtaagctcga
    41941 gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga cgggggccgc
    42001 ctgcccccat caaattcaga cacagaatca tcaacatagc ggtggaaggc agcacgttta
    42061 cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag ttgtaaattt
    42121 cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc ggccagacga
    42181 acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa accagcgttg
    42241 aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg acgcgacggg
    42301 cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt tcctccccat
    42361 gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac attcagaaac
    42421 aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc gggtgagcca
    42481 ctttcgtagc tcacccgtta gcact
//
LOCUS       DAZPEU010000017        40398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPEU010000017 DAZPEU010000000
VERSION     DAZPEU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.017"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            339..425
                     /gene="leuL"
                     /locus_tag="ACHU2L_000798"
     CDS             339..425
                     /gene="leuL"
                     /locus_tag="ACHU2L_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR1355775.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            518..2089
                     /gene="leuA"
                     /locus_tag="ACHU2L_000799"
     CDS             518..2089
                     /gene="leuA"
                     /locus_tag="ACHU2L_000799"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR1355776.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            2089..3180
                     /gene="leuB"
                     /locus_tag="ACHU2L_000800"
     CDS             2089..3180
                     /gene="leuB"
                     /locus_tag="ACHU2L_000800"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR1355777.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3183..4583
                     /gene="leuC"
                     /locus_tag="ACHU2L_000801"
     CDS             3183..4583
                     /gene="leuC"
                     /locus_tag="ACHU2L_000801"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR1355778.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4594..5199
                     /gene="leuD"
                     /locus_tag="ACHU2L_000802"
     CDS             4594..5199
                     /gene="leuD"
                     /locus_tag="ACHU2L_000802"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR1355779.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5248..6426)
                     /gene="setA"
                     /locus_tag="ACHU2L_000803"
     CDS             complement(5248..6426)
                     /gene="setA"
                     /locus_tag="ACHU2L_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR1355780.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6528..6659)
                     /gene="sgrT"
                     /locus_tag="ACHU2L_000804"
     CDS             complement(6528..6659)
                     /gene="sgrT"
                     /locus_tag="ACHU2L_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR1355781.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6748..8403
                     /gene="sgrR"
                     /locus_tag="ACHU2L_000805"
     CDS             6748..8403
                     /gene="sgrR"
                     /locus_tag="ACHU2L_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR1355782.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8437..8536
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8567..9550
                     /gene="thiB"
                     /locus_tag="ACHU2L_000806"
     CDS             8567..9550
                     /gene="thiB"
                     /locus_tag="ACHU2L_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR1355783.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9526..11136
                     /gene="thiP"
                     /locus_tag="ACHU2L_000807"
     CDS             9526..11136
                     /gene="thiP"
                     /locus_tag="ACHU2L_000807"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR1355784.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11120..11818
                     /gene="thiQ"
                     /locus_tag="ACHU2L_000808"
     CDS             11120..11818
                     /gene="thiQ"
                     /locus_tag="ACHU2L_000808"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR1355785.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11932..12696)
                     /locus_tag="ACHU2L_000809"
     CDS             complement(11932..12696)
                     /locus_tag="ACHU2L_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1355786.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12782..13660)
                     /gene="araC"
                     /locus_tag="ACHU2L_000810"
     CDS             complement(12782..13660)
                     /gene="araC"
                     /locus_tag="ACHU2L_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR1355787.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13999..15699
                     /gene="araB"
                     /locus_tag="ACHU2L_000811"
     CDS             13999..15699
                     /gene="araB"
                     /locus_tag="ACHU2L_000811"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR1355788.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15710..17212
                     /gene="araA"
                     /locus_tag="ACHU2L_000812"
     CDS             15710..17212
                     /gene="araA"
                     /locus_tag="ACHU2L_000812"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR1355789.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17412..18107
                     /gene="araD"
                     /locus_tag="ACHU2L_000813"
     CDS             17412..18107
                     /gene="araD"
                     /locus_tag="ACHU2L_000813"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR1355790.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLHKH
                     YLRKHGAKAYYGQ"
     gene            18182..20533
                     /gene="polB"
                     /locus_tag="ACHU2L_000814"
     CDS             18182..20533
                     /gene="polB"
                     /locus_tag="ACHU2L_000814"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR1355791.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20698..23604
                     /gene="rapA"
                     /locus_tag="ACHU2L_000815"
     CDS             20698..23604
                     /gene="rapA"
                     /locus_tag="ACHU2L_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR1355792.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23616..24275
                     /gene="rluA"
                     /locus_tag="ACHU2L_000816"
     CDS             23616..24275
                     /gene="rluA"
                     /locus_tag="ACHU2L_000816"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR1355793.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24392..25207)
                     /gene="djlA"
                     /locus_tag="ACHU2L_000817"
     CDS             complement(24392..25207)
                     /gene="djlA"
                     /locus_tag="ACHU2L_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR1355794.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25462..27816
                     /gene="lptD"
                     /locus_tag="ACHU2L_000818"
     CDS             25462..27816
                     /gene="lptD"
                     /locus_tag="ACHU2L_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR1355795.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27869..29155
                     /gene="surA"
                     /locus_tag="ACHU2L_000819"
     CDS             27869..29155
                     /gene="surA"
                     /locus_tag="ACHU2L_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR1355796.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29155..30144
                     /gene="pdxA"
                     /locus_tag="ACHU2L_000820"
     CDS             29155..30144
                     /gene="pdxA"
                     /locus_tag="ACHU2L_000820"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR1355797.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30141..30962
                     /gene="rsmA"
                     /locus_tag="ACHU2L_000821"
     CDS             30141..30962
                     /gene="rsmA"
                     /locus_tag="ACHU2L_000821"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR1355798.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30965..31342
                     /gene="apaG"
                     /locus_tag="ACHU2L_000822"
     CDS             30965..31342
                     /gene="apaG"
                     /locus_tag="ACHU2L_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR1355799.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31349..32191
                     /gene="apaH"
                     /locus_tag="ACHU2L_000823"
     CDS             31349..32191
                     /gene="apaH"
                     /locus_tag="ACHU2L_000823"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR1355800.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32269..32748)
                     /gene="folA"
                     /locus_tag="ACHU2L_000824"
     CDS             complement(32269..32748)
                     /gene="folA"
                     /locus_tag="ACHU2L_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR1355801.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32939..34801)
                     /gene="kefC"
                     /locus_tag="ACHU2L_000825"
     CDS             complement(32939..34801)
                     /gene="kefC"
                     /locus_tag="ACHU2L_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR1355802.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34794..35324)
                     /gene="kefF"
                     /locus_tag="ACHU2L_000826"
     CDS             complement(34794..35324)
                     /gene="kefF"
                     /locus_tag="ACHU2L_000826"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR1355803.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35372..36766)
                     /locus_tag="ACHU2L_000827"
     CDS             complement(35372..36766)
                     /locus_tag="ACHU2L_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1355804.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36825..37112)
                     /gene="fixX"
                     /locus_tag="ACHU2L_000828"
     CDS             complement(36825..37112)
                     /gene="fixX"
                     /locus_tag="ACHU2L_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR1355805.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37109..38395)
                     /locus_tag="ACHU2L_000829"
     CDS             complement(37109..38395)
                     /locus_tag="ACHU2L_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1355806.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38446..39387)
                     /locus_tag="ACHU2L_000830"
                     /pseudo
     CDS             complement(38446..39387)
                     /locus_tag="ACHU2L_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39402..40172)
                     /locus_tag="ACHU2L_000831"
     CDS             complement(39402..40172)
                     /locus_tag="ACHU2L_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR1355807.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9746 a        11150 c        10582 g         8920 t
ORIGIN      
        1 taattgttca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac atataattca
       61 atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt tcctcgttga
      121 ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg tagttaagca
      181 gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta cggatgcaga
      241 actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt gtatccagta
      301 actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc gttcgcttta
      361 tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc ggcatccagc
      421 attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt ttttatgccc
      481 gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag tcattatttt
      541 cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg tgaaagaaaa
      601 actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag tcggtttccc
      661 cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg ttaaaaacag
      721 ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg ccgaatccct
      781 gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa tgcacatcgc
      841 caccaagctg cgcagcaccc tagacgaggt gatcgaacgc gctatctata tggtgaaacg
      901 cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc gtacacccat
      961 tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca ccatcaacat
     1021 tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca gcggcctgta
     1081 tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg acgatttagg
     1141 cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg tggaaggtgc
     1201 aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca tcatggcgat
     1261 taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccaag agatatggcg
     1321 caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca aagccattgt
     1381 tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc tgaaaaaccg
     1441 cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc agctgaatct
     1501 gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg ggtataaaga
     1561 aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg acaaaaaagg
     1621 tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc aagaagagcc
     1681 ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata tcgccaccgc
     1741 cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg gtaacggtcc
     1801 ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg aactggtgaa
     1861 atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg atattgtcac
     1921 caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg tcgagtcctc
     1981 cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag tcgaaaaaga
     2041 gttgcaacgc aaaactcaac acaacgaaaa caacaaggaa accgtgtgat gtcgaagaat
     2101 taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac ccaggcgctg
     2161 aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag ccattacgat
     2221 gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac ggttgaaggt
     2281 tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg ggaacattta
     2341 ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca cttcaaatta
     2401 ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg tccgctgcgt
     2461 gcagacattg ccgctaacgg cttcgacatc cagtgcgtgc gcgaactgac cggcggcatc
     2521 tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc gtttgatacc
     2581 gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga atctgctcgt
     2641 aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc ctctatttta
     2701 tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact ggcgcatatg
     2761 tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga cgttctgctg
     2821 tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac tggctcgatg
     2881 ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga accggcgggc
     2941 ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca aatcctgtcg
     3001 ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc cattgaacgc
     3061 gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg tggcgctgcc
     3121 gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga aggggtgtaa
     3181 tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac gaagccgaaa
     3241 acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc tcaccgcagg
     3301 cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa accttcgcca
     3361 ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt gaaatggcgc
     3421 gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag ctgtatgacc
     3481 tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc gtcaccttgc
     3541 cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg tttggcgcac
     3601 tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa accctgaaac
     3661 agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg ggcattaccg
     3721 caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc accgggcatg
     3781 tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt atgaccctgt
     3841 gcaatatggc gatcgaaatg ggcgcaaaag ccgctctggt tgctcctgac gaaaccacct
     3901 ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac gacgccgttg
     3961 cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt gtcactctgc
     4021 aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag gtgatttctg
     4081 tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt gcgtcggcag
     4141 aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa gtggctatcg
     4201 acaaagtgtt tatcggttcc tgtaccaact ctcgcattga agatttacgc gcggcagcgg
     4261 agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt cccggctctg
     4321 gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt gaagccggtt
     4381 ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac cgtctgaatc
     4441 cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag gggcgcggcg
     4501 ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc ggacatttcg
     4561 ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat caaacacaca
     4621 ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat cccgaaacag
     4681 tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga ctggcgtttt
     4741 ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc gcagtatcag
     4801 ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg tgagcacgcg
     4861 ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt tgctgacatc
     4921 ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga tgcagaagtg
     4981 gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt ggatctggaa
     5041 gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc cttccgccgc
     5101 cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga cgacgctatt
     5161 gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc ggtcaaatga
     5221 ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg ttaaaaataa
     5281 tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc aactttgtgc
     5341 aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt tggtaaataa
     5401 ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca tcccaatacc
     5461 cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg ccatacggct
     5521 atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa tgaccatcat
     5581 tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg gtatttccag
     5641 tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta aatcgctact
     5701 gatccacaat ggcatatcaa taatgtacat agtgttgcag gtccacatta acgtcgaggc
     5761 gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata aagcattttc
     5821 cgacggcagt tctacccgcg ccacagacag aagcataaat gcaatcaata ccagactgag
     5881 tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca acgccagcat
     5941 aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca tcaccgagct
     6001 aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag caaataactg
     6061 tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa gcgtcagata
     6121 atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc aaaatataat
     6181 cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc ttacgccgat
     6241 cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg cgccaacctc
     6301 acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc ccatcataaa
     6361 agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg tcattatcca
     6421 gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt ataatctgct
     6481 ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca actttcagaa
     6541 ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg gacgcttaac
     6601 caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa ctgacgcatg
     6661 gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa ataaaattgc
     6721 tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca acagttcatc
     6781 cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga actggcagcg
     6841 ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca ggatcgcggc
     6901 tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac attcctctat
     6961 accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga tcgtatcgac
     7021 caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt ttctcatctg
     7081 ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg tccgttgcgt
     7141 aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg gcaaatcttc
     7201 agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat cgcccaccac
     7261 tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt ccattttcat
     7321 catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat caatacgctg
     7381 ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct ggatatccat
     7441 ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc gatgatcctg
     7501 ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac cggtccgtat
     7561 gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga cttcttcggt
     7621 taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc cgacgagcca
     7681 gccggtggtc tgatgctaaa aggtccacag ggcgagaaaa aagagattga aagccgcctg
     7741 gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc gaatcagcaa
     7801 gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt cgctgaggaa
     7861 cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca ccatgcccgc
     7921 accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt ttatcaggat
     7981 cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag tcaccaggtc
     8041 acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat cgagagcgat
     8101 atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt cgcgcacttg
     8161 tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga cgccgcccgc
     8221 tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc cagtaaagca
     8281 atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat gcgcggcctg
     8341 cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc accggatcca
     8401 tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg gtgccacgca
     8461 aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg cgaagggatt
     8521 tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt taaaaaaatg
     8581 tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc tgactgttta
     8641 tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa aagcttttga
     8701 agccaactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt cgcttctcaa
     8761 ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc tggataacaa
     8821 tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg cagcggatgc
     8881 cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt acggctattt
     8941 cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga aagaattggt
     9001 tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca cgccggggct
     9061 gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag cctggcaaaa
     9121 actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg gcctgttttt
     9181 aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc acattctcga
     9241 agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc tgcaggtgga
     9301 agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat tcctccagtt
     9361 tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt atccggtggc
     9421 aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca cgctggagtt
     9481 cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc aacgcgccgt
     9541 cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct ggtggtagcg
     9601 gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa ctgggtggca
     9661 gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca ggcgtttctc
     9721 tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta tcgcaggcgc
     9781 tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct cccggtgcta
     9841 gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc atcgctctgc
     9901 caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat tttgctggcg
     9961 cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact ggaaaacatt
    10021 cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca ttttttccgc
    10081 ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct tatctttatg
    10141 ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca ggcaaccact
    10201 atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg cgcggcgatg
    10261 ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca gcgactaagt
    10321 aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga cgatcgtctg
    10381 catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt gctgccaccg
    10441 ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct ggcacaaccg
    10501 gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg tgtattgtgc
    10561 gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg gcagaaaatg
    10621 ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc ggggattgtg
    10681 ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca atctgctgac
    10741 ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa agtactggaa
    10801 aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct gggcattgaa
    10861 ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact ggcgcaggcg
    10921 ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt gttcggtaac
    10981 gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta tcgcagccag
    11041 gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt taccgtgatt
    11101 gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt atcaccattt
    11161 gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc tcgggccaag
    11221 cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc cagccagcgg
    11281 ttcgctgact atcgatggtg ttgatcacac cacgacgccg ccgtcacgcc gtccggtgtc
    11341 gatgctgttt caggagaaca acctgttcag ccatctgacg gtcgcgcaga acatcgggct
    11401 ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc acgcgattgc
    11461 ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt ccggcggtca
    11521 gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt tattgctcga
    11581 tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc tggtgagcac
    11641 gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg aagatgcggc
    11701 gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc agggtaagac
    11761 tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta cgggttagta
    11821 tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg ttttgtaggc
    11881 ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc cctaaacccc
    11941 aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa ttaacaccac
    12001 cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac ggccgcgcga
    12061 caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc agcacagcca
    12121 gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact cgccgctctg
    12181 cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga agtaaaacgg
    12241 tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg gcagatccag
    12301 cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac cgaccagaat
    12361 ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg ctttgttttt
    12421 cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc agaaggagat
    12481 ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga aacttaactc
    12541 gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta gaatcaaacc
    12601 gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg aatacacggt
    12661 agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa atggacgaag
    12721 cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca
    12781 attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc
    12841 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac
    12901 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct
    12961 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa
    13021 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat
    13081 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga ttatccatcg
    13141 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat
    13201 tcatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa
    13261 acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc gtattggcaa
    13321 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact
    13381 ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct cctggcggga
    13441 acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc accaccccct
    13501 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa
    13561 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg
    13621 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat
    13681 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
    13741 ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg taacaaagca
    13801 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
    13861 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
    13921 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt
    13981 tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag tgattctgtg
    14041 cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt agagtggtat
    14101 ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg tcatcatccg
    14161 cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga gcttagcgtc
    14221 gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac gcccgcaccg
    14281 attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa cccgaacgcg
    14341 atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat tacccgtttg
    14401 tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta ttccagcgaa
    14461 tggttctggg caaaaatcct gcatgtgact cgccaggaca gcgccgtggc gcaatctgcc
    14521 gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac cacccgcccg
    14581 caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca cgaaagctgg
    14641 ggcggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa tcgccatttg
    14701 ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac cttatgcccg
    14761 gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg cgcgtttgac
    14821 tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa agttatcggt
    14881 acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg ggcagttaaa
    14941 ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat ttatcggtct ggaagcaggc
    15001 caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg gccgctggaa
    15061 cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca gaaacaactg
    15121 cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct gccggtggtg
    15181 ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa aggggtgatt
    15241 accgatctta acctcgctac cgacactccg ctgctgttcg gcggtttgat tgctgccacc
    15301 gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc cgtcaataac
    15361 gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc ctgctgcgac
    15421 gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct cggtgcggcg
    15481 atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca gcaaaaaatg
    15541 gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg ctttgaacag
    15601 ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct tccaacttcc
    15661 gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa tggcgatttt
    15721 tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc cggaaaccct
    15781 gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg aagcgaaact
    15841 gccctacaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca ccgctatttg
    15901 ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc acaccttctc
    15961 cccggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc tgcaattcca
    16021 cacccagttc aacgcggcgc tgccgtggga cagtatcgat atggacttta tgaacctgaa
    16081 ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc gtcagcaaca
    16141 tgccgtcgtt accggtcact ggcaggataa acaagcccat gagcgtatcg gctcctggat
    16201 gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgat ttggcgataa
    16261 catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca agttcggttt
    16321 ctccgtcaat acctgggcgg ttggctatct ggtgcaggtg gtgaactcca tcagcgacgg
    16381 cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc ctgccacaca
    16441 aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc tggggatgaa
    16501 gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag atttgcacgg
    16561 tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt acggctttgc
    16621 gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga tgtcaaccgg
    16681 tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga aaggtaatga
    16741 cctggtgctc ggctcccata tgctggaagt ctgcccgtcg attgccgtag aagagaaacc
    16801 gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc gactgatctt
    16861 caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg atcgttaccg
    16921 tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga aactgccggt
    16981 ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact gcttccgaag cgtggatcct
    17041 cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg atatgcgcca
    17101 gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc gcctgccagc
    17161 gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct aagtagccac
    17221 atcaggtatg taacgcctga tgcgacgctg acgcgtctta tctggcctac acgctgcgat
    17281 tttgtaggcc ggataagcaa agcgcatccg gcattcaacg cctgatgcga cgctggcgcg
    17341 tcttatcagg cctacgcgct gcgattttgt agaccggata agcgaagcgc atccggcacg
    17401 aaggagtcaa catgttaaaa gatctcaaac gccaggtatt agaagccaac ctggcgctgc
    17461 caaaacacaa cctggtcacg ctcacatggg gcaacgtcag cgccgttgat cgcgagcgcg
    17521 gcgtctttgt gatcaaacct tccggcgtcg attacagcgt catgaccgct gacgatatgg
    17581 tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc tcctccgaca
    17641 cgccaactca ccggctgctc tatcaggcat tcccctccat tggcggcatt gtgcatacgc
    17701 actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca accggcacca
    17761 cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc gacgcagaaa
    17821 tcaacggcga atatgagtgg gaaaccggta acgtcatcgt agaaaccttt gaaaaacagg
    17881 gtatcgatgc agcgcaaatg cccggcgttc tggtccattc ccacggcccg tttgcatggg
    17941 gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc gcttatatgg
    18001 ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg ctgctgcata
    18061 aacactatct gcgtaagcat ggcgcgaagg catattacgg gcagtaatga ctgtataaaa
    18121 ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg gttttttgat ggattttcag
    18181 cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc aagggacaga
    18241 agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg caccgcaaga
    18301 gtccgtggcg tttattcacg ccgatcaggt tccccgcgct cagcatattt tgcagggtga
    18361 acaaggcttt cgtctgacac cgctggcgtt aaaggatttt caccgccagc cggtgtatgg
    18421 cctttactgt cgcgcccatc gccaattgat gaattacgaa aagcgtctgc gtgaaggtgg
    18481 cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg agcggtttat
    18541 cacctcaccg gtgtgggtcg agggtgatat gcacaatggc actatcgtta atgcccgtct
    18601 gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctatagata ttgaaaccac
    18661 ccgccacggt gagctgtact gcatcggcct ggaaggctgc gggcagcgca tcgtttatat
    18721 gctggggccg gagaatggcg acgcctcctc gcttgatttc gaactggaat acgtcgccag
    18781 ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca aaccacgatc ccgatgtgat
    18841 catcggctgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg ccgagcgtta
    18901 ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg agcacggctt
    18961 taaaaacggc gtcttttttg cccaggctaa aggtcggcta attatcgacg gtatcgaggc
    19021 gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg ctcaggagct
    19081 attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa ttgaccgccg
    19141 tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg agctggtgac
    19201 gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcaa cggtgaacgg
    19261 cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct attttccgcg
    19321 aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc acgccagccc
    19381 tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg tgctggacta
    19441 taaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg ggctggttga
    19501 aggcatggcg caacctgatc cagagcacag taccgaaggt tttctcgatg cctggttctc
    19561 acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc gcgataaagc
    19621 caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga atgcctttta
    19681 tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat cgtcgatcac
    19741 catgcgtggt catcagatca tgcggcagac caaagcattg attgaagcgc agggctacga
    19801 cattatctac ggagataccg actcgacttt tgtctggcta aaaggtgcac attcggaaga
    19861 agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt gggcggaaac
    19921 gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaactc atttctgccg
    19981 ttttcttatg ccaaccattc gcggagctga taccggcagt aaaaagcgct acgccgggct
    20041 gattcaggag ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg tgcgcaccga
    20101 ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct tccgcaacga
    20161 gccatatcag gagtacgtcc gccaaaccat cgacaaactg atggcgggtg aactggatgc
    20221 gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc gtaatgttcc
    20281 gcctcatgta cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg gtcgcccctt
    20341 gcaatatcag aatcgcggca ccattaagta cgtatggacc accaacggcc cggagccgct
    20401 ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc tacaacccgt
    20461 ggcggaggga atactccctt ttattgagga taattttgct acacttatga ccgggcaact
    20521 tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg tgacgaatgc
    20581 tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca ccactacctc
    20641 ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg aacacatatg
    20701 ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg acttggaacc
    20761 gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg tgaaaaccgt
    20821 ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg tgataccatt
    20881 accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa tggcttgctg
    20941 acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg tgaagttttc
    21001 cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg gcagattgac
    21061 cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga acagttccgt
    21121 atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca gctcaacatc
    21181 gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga agtgggttta
    21241 gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg cgctgctgaa
    21301 cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga aatgctgcgc
    21361 cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc tcagcacgat
    21421 gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt tgcccgtcgt
    21481 agcaaacagc gcttggaaca tctctgtgaa gccgaatggg acctgctggt ggtcgatgaa
    21541 gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc cattgaacaa
    21601 ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca gctggggatg
    21661 gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga ttttgcgcag
    21721 ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat gctgctggca
    21781 ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg cgagcaggat
    21841 atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag cgcccgtcag
    21901 gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt ccgtaacacg
    21961 cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct gccgctaccg
    22021 acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa aagtgcggaa
    22081 gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga aggtgataac
    22141 gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct gaccagccat
    22201 cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca actggagcag
    22261 gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat gtcgattatc
    22321 gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca ggtactgctg
    22381 tgctcagaaa tcggttctga aggacgtaac tttcagttcg ccagtcacat ggtgatgttt
    22441 gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga tcgtatcggc
    22501 caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca gtcggtgctg
    22561 gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac cggacgcact
    22621 atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga tcaaaccgaa
    22681 ggctttgacg atctgatcaa aagctgccgc gagcaacatg aagcgctgaa agcacagctg
    22741 gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa agcccaggca
    22801 ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt cgccatgaac
    22861 ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat cgtgctgacg
    22921 ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg catcaccatc
    22981 acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac ctgggagcat
    23041 ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag cagcacgatt
    23101 tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggtggaact gatttatgtg
    23161 gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc gacgccggta
    23221 cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt tgaaaccttt
    23281 aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa cgccgtgcag
    23341 caggatgttc acgctatcct tcaactgggt gaagcgcaga tcgagaaatc tgcccgtgca
    23401 ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct gtctcgtctg
    23461 gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc cattgagagc
    23521 aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga tgccctgcgt
    23581 ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac tacaatccac
    23641 cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg gtcaacaagc
    23701 cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc gtgatgacgc
    23761 gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg gctaccagcg
    23821 gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc cagttccgcg
    23881 agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc cccgcagaag
    23941 gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag aaagtctgtt
    24001 acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg gcggataaca
    24061 cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt gtgcatatgc
    24121 tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa gcgagagcga
    24181 tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg gcgtatggca
    24241 atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc agttcggtag
    24301 gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga tgcgacgcgg
    24361 gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc tgctttatca
    24421 gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc atctcaggcg
    24481 gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta cggtaggcac
    24541 gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag gcatcttcca
    24601 gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc tgataaccgc
    24661 cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg gcgcgggaga
    24721 tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc agtgaaccat
    24781 cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca aaacgaccaa
    24841 agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct gattttccca
    24901 cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt cggtccatca
    24961 actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg gttaaatgcc
    25021 ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg ttggcgaacc
    25081 acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg cctaacacta
    25141 cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt tttccccaat
    25201 actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac aatataaagg
    25261 tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat ttgcattatc
    25321 atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag gattaacact
    25381 agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac gttaccgatg
    25441 atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc caccatgatt
    25501 gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca gtgcatgttg
    25561 ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt acccgtgact
    25621 atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac tggcagcgtg
    25681 gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca tcaaaaagag
    25741 gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt ccattacgac
    25801 gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac caaagatacc
    25861 aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa agcggacctg
    25921 atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt tacctcctgt
    25981 ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga ccgcgaagaa
    26041 caagttgctg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat cttttatagc
    26101 ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat cccgaacgcc
    26161 aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa catcgcgcca
    26221 aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat catgtgggag
    26281 aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga ctatctgcct
    26341 tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg ttggttattc
    26401 tactggaacc actccggggt catggatcag gtgtggcgtt tcaacgtcga ctacaccaag
    26461 gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag tactgacggc
    26521 tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc caccgtttca
    26581 accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc agagccgcag
    26641 ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat ttacggccag
    26701 gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt tcacctggaa
    26761 ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga agcgaagttt
    26821 ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa cacgaccaag
    26881 ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa aatggtcttt
    26941 gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc gcgcgcgcag
    27001 tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc gtctctgctg
    27061 caatctgact actctggcct gttccgggac cggacttacg gcggtcttga ccgtattgcc
    27121 tccgctaacc aagtgacgac cggtgtcaca tctcgcatat atgatgatgc tgccgttgaa
    27181 cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac tggcgatgac
    27241 aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg cgacacctac
    27301 tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac acgtctggat
    27361 aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg tctggtacag
    27421 ctgaattacc attacgccag cccggaatat attcaggcta cgctgcctaa gtactattcc
    27481 actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag caggccaatt
    27541 gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa caagcaagcc
    27601 gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt cggttacgag
    27661 cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa cgcaatcggc
    27721 tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca agagatgctg
    27781 cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc acgtaatccg
    27841 cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc ttctcggtat
    27901 cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag tcgcagccgt
    27961 cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt cggtaaaact
    28021 gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc accaaatcat
    28081 ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg gagtgaaaat
    28141 ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca acatgacgct
    28201 ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacactt atcgtaacca
    28261 gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc gtcgcatcac
    28321 catcctgcca caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa acgacgccag
    28381 cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga cctctgatca
    28441 ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta acggcgctga
    28501 tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg gcggccagat
    28561 ggggtggggc cgtattcagg agttgccggg gatcttcgcc caggcattaa gcaccgcgaa
    28621 gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc tgaaagttaa
    28681 cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc gccatattct
    28741 gctgaaaccg tcgccgatca tgactgacga acaggcccgc gtgaaactgg agcaaattgc
    28801 tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt tctcgcagga
    28861 tccaggttct gctaaccagg gcggtgatct cggctgggct acaccagata ttttcgatcc
    28921 agccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac cggttcactc
    28981 ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata aaaccgacgc
    29041 tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg aagaagcagc
    29101 aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca actaatggtt
    29161 aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc ggacttagtt
    29221 gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga tgccactctc
    29281 cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta ttcccccaac
    29341 tcccctgcac aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc gctacgtgaa
    29401 tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga gacgctggcg
    29461 cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg ccccgtgcat
    29521 aaaggcgtca ttaacgacgc aagcattccg tttaccggtc ataccgagtt tttcgaagag
    29581 cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg cgtggcgctg
    29641 gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc acttttgcac
    29701 gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc cgaaccgcgc
    29761 attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg tacggaagag
    29821 atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa actcaacggg
    29881 ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga cgccgtgctg
    29941 gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg gcgcggtgtg
    30001 aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac cgcgcttgaa
    30061 ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa tctcgccatc
    30121 aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta gcccgtaaac
    30181 gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt tctgccatta
    30241 acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca ttgaccgagc
    30301 cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat ctggcggcac
    30361 gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag gatgcgatga
    30421 cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt ttcggcaacc
    30481 tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact gatgccattg
    30541 ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca ggaccgaaca
    30601 gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg atcccggtac
    30661 tggaagttcc gccgtcagcc tttacaccac cacccaaagt ggattccgcc gtcgtgcgcc
    30721 tggtccctca tgcaacgatg cctcacccgg ttaaagatat tcgcgtgttg agccgcatca
    30781 ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc aacctgttta
    30841 gcgtcgaggt gttaaccgga atggggatcg acccggcgat gcgagcggaa aatatctctg
    30901 tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg caggagagtt
    30961 aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca ttgaggcaca
    31021 atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac gcaatctggg
    31081 gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca atggccgtga
    31141 aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc ctggcgaaga
    31201 gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc agggtcacta
    31261 cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat tccgactcgc
    31321 cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg acgttcatgg
    31381 ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccccg ggaaagatac
    31441 cctctggctg acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg ttctgcgcta
    31501 tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc tgcatctgct
    31561 ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac cgctgctgga
    31621 agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc tgcaaatcga
    31681 cgaagagaaa aagctggtga tggcccacgc agggatcacg ccgcagtggg atctgcagac
    31741 cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct atcccttctt
    31801 tcttgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc gggggctggg
    31861 aagactgcgt tttatcacca acgcctttac ccgtatgcgt ttttgcttcc cgaacggtca
    31921 actggatatg tacagcaaag aatcgccgga agaagcccct gccccactga aaccgtggtt
    31981 tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact gggcatcgct
    32041 ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat accggctgct gctggggtgg
    32101 gtcattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt cgaaccggca
    32161 taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatacaggaa ggccggataa
    32221 gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaactt accgccgctc
    32281 cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga attcgctgaa
    32341 taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt ccacttctgc
    32401 gtcgatatgc gtcagataca gtttttgcgc ttttggcagg aactgctcat aaacgcgacc
    32461 gccgccaatc accatgattt ctggtacgtc accacacgcc gcaatggctt catccaccga
    32521 tttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa tatttttgcg
    32581 tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca cgggtttatt
    32641 taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca tggcgttttc
    32701 catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg agatttcccg
    32761 ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg tgcgaggatg
    32821 atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg gactcgccgc
    32881 attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc actctttatt
    32941 aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta ccttcttctg
    33001 ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg gtgatgatct
    33061 cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg tcattctcaa
    33121 ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg gcacgttctc
    33181 gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt ttcagcgcac
    33241 cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga atgtagtgat
    33301 cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc accatctccg
    33361 tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc gctttcgccg
    33421 ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc ataaaacact ttcataccaa
    33481 atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt ttcaccccgc
    33541 tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg ataatcacgc
    33601 gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa gattgctcaa
    33661 ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc accgccaggg
    33721 tcagcgattt cacccactcc ggctccagca cattcgccat ctgcgccgca ccaaatacca
    33781 caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc tgtttatttg
    33841 gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg aggaaaccga
    33901 gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct atcgacatgc
    33961 caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata tcgctttcca
    34021 acgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg cccatcgcca
    34081 tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat aacgccacgg
    34141 cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga cgcgtaacat
    34201 agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc gagagagcaa
    34261 atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc gccaccagcg
    34321 ggattgccgc gatatcctgg aacagcagca ctgcaaaggc actgcggccc atttgcgtca
    34381 ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgttgatgag agcgccagcg
    34441 tcataccgat caattccgcg acctgccagc gcagcccaag taacatacag aacagcccca
    34501 gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc gcacgcagct
    34561 tccacagcct ttgtggatcg agttcgaggc cgataataaa cagcatcagc accaccccaa
    34621 tctcggcaaa gtgcagaata gattcggcat cggtcaccag tcgcagcccc cacgggccaa
    34681 taatgcagcc ggcgatcagg tagccaagta ccgatcccag accaagacgt accgcaatgg
    34741 gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta tgactatcca
    34801 tgatgggcct cctgccattc cagcagacgt tgcttatagt ggcgcgcctg cccttcgagg
    34861 gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca gttcagcccg
    34921 cagtagattg ccgtcgcctg tagcggctgc gacagcacat caaagcccgg atgcgcacca
    34981 atttcaaaat ggctttcccc gccgccggtc gtcaccgccc acagcaaatg tttgccatgc
    35041 agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca aaactttatc gatccaaagt
    35101 ttaagaagcg gaggaatgct gtaccactgc atcgggtgct gccagacgat cagatcggcg
    35161 cgagagagcg cctcctgctc ggcggcaata tcgatattga agtcaggata gagttgataa
    35221 agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt caagcatccg tttattcgca
    35281 tgggaatgat gcggatacgg atgcgcataa atgatgagga tcatagttat tagcccgcgt
    35341 gtttacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt atagtcaata
    35401 atattgattc agaaaagaga gaaatctgaa caaataatcc cattctctgc ccgcggatcg
    35461 tcatattgct ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac gctacggaaa
    35521 tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg ataccgtaat
    35581 tattgataaa tatcggcagt gcccaggtcg aaacaatggt gccaatacga cttaaggaca
    35641 taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagttcatta ggatagagcc
    35701 actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc atcactacca
    35761 gccagatccc catatccggg attagcccca aaaccgccaa cgccagcgtc atcatggcaa
    35821 agctgccaat caacaatgga cgccgtccgg cagtgtttag ccacagcatc ggcggaatac
    35881 agccgagcat aaagaacagg ctaatcacca cattccctag tgccgcgttt ttgccaaccc
    35941 ccaatcccaa cgaaccaacg atttgcgggc caaaggtgta aatggcgaac attgggatca
    36001 cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg cgattaaaca
    36061 gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt tcgccaaaca
    36121 gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac cagcggggag
    36181 attctggcag ttcgaatcga ccaatcaaaa tcaacaaaca agggatcgcc gcgctaccca
    36241 gcatccagcg ccaaccacct tccacatcat aaagccagta gccgaccaga tcggcgcagg
    36301 tcgcgccgac ataccacatc gcggcaataa agctgatgga aaacgcccgc tgacgggtac
    36361 tggagaactc ggtgatcatc gaagtggcga tgggataatc tgcaccgatg acaatgccga
    36421 tgagtacccg catcaccaac agttcgacgg gggatgaaac acacatcgtc gccaccgata
    36481 tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact ttatcggaga
    36541 tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg cccagcaagc
    36601 caatccagtc agcgtccagt ttcagcgccg gagtcagttg ctccagcgcc acgccaatca
    36661 ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa atgcggcggt
    36721 gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt tattcctgtt
    36781 tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc gtaacggaac
    36841 tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc cagccccaga
    36901 atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact gccgtcatcc
    36961 tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcac ctgtttatca
    37021 gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt attgacgccc
    37081 agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc atgccatcct
    37141 tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg cgcatcagtt
    37201 ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc agttccgggt
    37261 tgccgctaaa catgcgtggg ttatcaagga acgccggtag tttctggtac atgcgcatat
    37321 cgcgcagcgg gccactctcc agatgctgac gatattccgc gagtttttgc ttactgaaat
    37381 cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg gcaatcgcca
    37441 gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca tcaccggcaa
    37501 tcaatacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc ggcactacgt
    37561 gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgcaacggcc ggatgctgtt
    37621 tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc atgcagatga tgcagaccac
    37681 aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg cccatcaggc
    37741 catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc aactgaaaac
    37801 ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc gccacatccg
    37861 tcggtttaac gcgttttgcc atccccaact tttcggcaag gatggagttc accccatcag
    37921 caaggagcac cgttttcgct tcaatcacat caccatcggc ttctacaccg acgactttgc
    37981 catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg cccgcttctt
    38041 cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa gaacgctggg
    38101 atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt tccgtcataa
    38161 acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggaatcggcg aaacctggaa
    38221 taatgtgttc cagactgtgg gcatagagac gcccgccagt aacgttcttg gcacctgcgg
    38281 aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc agtgcggcaa
    38341 ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg gacataagag
    38401 ctccctgtca gcggcagagc cgtaatgcag ccctgccaga gtggatcaac gcgctaaagc
    38461 tgcggtcagt gccggaagga tcttcacggc atcgccaaca atgccgtaat ccgcgtactg
    38521 gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgacg cgttagcgcc
    38581 aaccatgtgc tggatctacc cggagatccc caccgccagg tacagttcag gtttcagcat
    38641 caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt ctgccaccgg
    38701 acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca gcgcaatgtt
    38761 ctctttgctg ccaatgccgc gaccgacgct gaccaccaga cgggctttgt cgagatcgac
    38821 gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg gagcctgcca
    38881 ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat cgaacgtgcc
    38941 gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa tcgccagacc
    39001 gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt acgctgacgg tgctggcatc
    39061 gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt tgccgcgacg
    39121 ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg cgaatagtgt cagccatgac
    39181 accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc agacatgatt
    39241 agcgccgagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg cattgatttg
    39301 attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaggggg tatcgctgaa
    39361 tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac tttgcgaaga
    39421 ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat gcgctgacgc
    39481 tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg cgtccacggc gttaaaacca
    39541 atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat ggctttcatc
    39601 gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg cagcggaatg
    39661 cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc tgccgtcagg
    39721 gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc caccagcaga
    39781 ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcag atcaaagcct
    39841 gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc ctgctcgaac
    39901 tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc tttacgccct
    39961 ttggcgttgg tcagggcttt accgcccaca cttaaggctg tcacctgcgc ctctgctgcc
    40021 tgttgcttta gctggcaagc cgcttcaata gcgttgagat cgtattggct tattttggca
    40081 tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc ctgttcatca
    40141 ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat gaaggaaacg
    40201 tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga aataaaactt
    40261 taatcaccaa tattgaaagt cagatgctat ttttttaccc acaaaagaat ataacaaact
    40321 gattaataac caaaattaac gaaaaacgcc ctctgttcac tcgaaacaaa aatgtgatac
    40381 caatcacaga atacagct
//
LOCUS       DAZPEU010000018        39978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPEU010000018 DAZPEU010000000
VERSION     DAZPEU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.018"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..155
                     /locus_tag="ACHU2L_000832"
     CDS             <1..155
                     /locus_tag="ACHU2L_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1355808.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="ACHU2L_000833"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="ACHU2L_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR1355809.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="ACHU2L_000834"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="ACHU2L_000834"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR1355810.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="ACHU2L_000835"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="ACHU2L_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="ACHU2L_000836"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="ACHU2L_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR1355811.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="ACHU2L_000837"
     CDS             complement(5626..7134)
                     /locus_tag="ACHU2L_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1355812.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHU2L_000838"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHU2L_000838"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR1355813.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="ACHU2L_000839"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="ACHU2L_000839"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1355814.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="ACHU2L_000840"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="ACHU2L_000840"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR1355815.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHU2L_000841"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHU2L_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR1355816.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHU2L_000842"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHU2L_000842"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR1355817.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="ACHU2L_000843"
     CDS             complement(14283..15602)
                     /locus_tag="ACHU2L_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR1355818.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHU2L_000844"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHU2L_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR1355819.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="ACHU2L_000845"
     CDS             16699..17034
                     /locus_tag="ACHU2L_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR1355820.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHU2L_000846"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHU2L_000846"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR1355821.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="ACHU2L_000847"
     CDS             complement(17790..19172)
                     /locus_tag="ACHU2L_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1355822.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHU2L_000848"
     CDS             complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHU2L_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR1355823.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="ACHU2L_000849"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="ACHU2L_000849"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR1355824.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="ACHU2L_000850"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="ACHU2L_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR1355825.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHU2L_000851"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHU2L_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR1355826.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHU2L_000852"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHU2L_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR1355827.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHU2L_000853"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHU2L_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR1355828.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="ACHU2L_000854"
     CDS             complement(25947..26768)
                     /locus_tag="ACHU2L_000854"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR1355829.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="ACHU2L_000855"
     CDS             complement(26868..26951)
                     /locus_tag="ACHU2L_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355830.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHU2L_000856"
     CDS             complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHU2L_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR1355831.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27623..27727
                     /locus_tag="ACHU2L_000857"
     CDS             27623..27727
                     /locus_tag="ACHU2L_000857"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR1355832.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27776..29029)
                     /locus_tag="ACHU2L_000858"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="ACHU2L_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30038
                     /locus_tag="ACHU2L_000859"
     CDS             29136..30038
                     /locus_tag="ACHU2L_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1355833.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30173..31393
                     /gene="mlc"
                     /locus_tag="ACHU2L_000860"
                     /gene_synonym="dgsA"
     CDS             30173..31393
                     /gene="mlc"
                     /locus_tag="ACHU2L_000860"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR1355834.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31518..32213
                     /gene="bioD"
                     /locus_tag="ACHU2L_000861"
     CDS             31518..32213
                     /gene="bioD"
                     /locus_tag="ACHU2L_000861"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1355835.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHU2L_000862"
     CDS             complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHU2L_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR1355836.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHU2L_000863"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHU2L_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR1355837.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHU2L_000864"
     CDS             complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHU2L_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR1355838.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHU2L_000865"
     CDS             complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHU2L_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR1355839.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..37796)
                     /locus_tag="ACHU2L_000866"
                     /pseudo
     CDS             complement(35758..37796)
                     /locus_tag="ACHU2L_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(37857..38891)
                     /locus_tag="ACHU2L_000867"
                     /pseudo
     CDS             complement(37857..38891)
                     /locus_tag="ACHU2L_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            38949..>39978
                     /locus_tag="ACHU2L_000868"
     CDS             38949..>39978
                     /locus_tag="ACHU2L_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1355840.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT        10163 a         9697 c        10237 g         9881 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctag
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt
    29761 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    29821 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    29881 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    29941 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    30001 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    30061 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    30121 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    30181 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    30241 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    30301 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    30361 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    30421 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    30481 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    30541 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    30601 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    30661 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    30721 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    30781 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    30841 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    30901 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30961 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    31021 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    31081 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    31141 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    31201 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    31261 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    31321 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    31381 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    31441 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    31501 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    31561 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    31621 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    31681 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    31741 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    31801 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    31861 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    31921 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31981 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    32041 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    32101 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    32161 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc
    32221 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    32281 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    32341 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    32401 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    32461 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    32521 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    32581 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgcctagt
    32641 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    32701 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    32761 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    32821 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    32881 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    32941 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    33001 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    33061 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    33121 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    33181 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgt gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc
    36121 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    36181 acatagccca tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc
    36241 aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca
    36301 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    36361 tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    36421 ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca
    36481 tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat
    36541 tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg
    36601 ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt
    36661 cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc
    36721 aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta
    36781 gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg
    36841 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    36901 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36961 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    37021 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    37081 ccgccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag tttcgtattg
    37141 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    37201 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    37261 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    37321 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    37381 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    37441 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    37501 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    37561 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    37621 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    37681 aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    37741 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    37801 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    37861 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    37921 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37981 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    38041 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    38101 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    38161 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    38221 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    38281 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    38341 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    38401 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    38461 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    38521 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    38581 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    38641 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    38701 atatcttcct gctcaacggt catcaggtct tccagcagaa tgtcggcata ttttgccgat
    38761 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    38821 tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag
    38881 atggactcca ttgccagacg gatggatgga cttgaagtag ttgactcaat gctacttaag
    38941 gtcctgatat gactgacatt aatcatgata acactgtata tccatccagt tctttctctg
    39001 ggtctcccta tcggtatcag caagtctaca cctcaagccc tgacacaagg ctgacacaaa
    39061 tcaatctact cagcgtagct aagcctcagt tagtccgcag agccaatggt cgatatacta
    39121 tccgcttcag actcaaggga cagaccacac cgtttctttc agtatcgacc cgcagcacag
    39181 acaggagagt agccacaatg cgtcagagag agcttgcagc caccgctaag gccttcatgt
    39241 tggataggcc tgaagtctca ctacaggagc ttacagagca tctccggtct atggctgagc
    39301 agttcctgac agacgctagt gacgattact ggaatggact tgaggtcgct actctggtcg
    39361 acgagaagtc caacctgaag gagctagccg ctacgcaagc cttgagtctg gaccagcaga
    39421 agggcataag gttagcgctg gaagtcctga ccgcagcaca gcagagagtc gacactggtg
    39481 atacctctgg tctgattaag ctgattgatg ataataacct tactgatgac tcaactatag
    39541 gcgactctac gtcaattctg aataatgagc agggggatag accggcagtt ttcacacagg
    39601 agcgccagtc atctgtagtt ttttcaagcc tagtgtcctc gctgctggct gagaaggtcc
    39661 agaccctgaa gacatccagc tacaaggacc tctcgtcctc actcaatact gtctcccggt
    39721 tcctgcctga agacatggac ctgatgtccc gctctgggtg gctggctgtc agggactcta
    39781 tgctggcttc tgaggtgaga ccatcgacaa tcaacaagct gctgaccaaa gcgaagatgt
    39841 gtctcgatta tggcctgatg aatgggcaac ttgagggccg caaccctatt gaacggatga
    39901 agctgaccaa ggatatcgac tccaagcgca gagcgttcac agacgaggag ctggagagac
    39961 ttctggtccg tgttgagt
//
LOCUS       DAZPEU010000019        39568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPEU010000019 DAZPEU010000000
VERSION     DAZPEU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.019"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(192..3638)
                     /gene="mfd"
                     /locus_tag="ACHU2L_000869"
     CDS             complement(192..3638)
                     /gene="mfd"
                     /locus_tag="ACHU2L_000869"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR1355841.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(3766..4839)
                     /locus_tag="ACHU2L_000870"
     CDS             complement(3766..4839)
                     /locus_tag="ACHU2L_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1355842.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            5101..6300
                     /gene="lolC"
                     /locus_tag="ACHU2L_000871"
     CDS             5101..6300
                     /gene="lolC"
                     /locus_tag="ACHU2L_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR1355843.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            6293..6994
                     /gene="lolD"
                     /locus_tag="ACHU2L_000872"
     CDS             6293..6994
                     /gene="lolD"
                     /locus_tag="ACHU2L_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR1355844.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            6994..8238
                     /gene="lolE"
                     /locus_tag="ACHU2L_000873"
     CDS             6994..8238
                     /gene="lolE"
                     /locus_tag="ACHU2L_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR1355845.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            8267..9178
                     /gene="nagK"
                     /locus_tag="ACHU2L_000874"
     CDS             8267..9178
                     /gene="nagK"
                     /locus_tag="ACHU2L_000874"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR1355846.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            9194..10015
                     /gene="cobB"
                     /locus_tag="ACHU2L_000875"
     CDS             9194..10015
                     /gene="cobB"
                     /locus_tag="ACHU2L_000875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR1355847.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(10171..11217)
                     /gene="potD"
                     /locus_tag="ACHU2L_000876"
     CDS             complement(10171..11217)
                     /gene="potD"
                     /locus_tag="ACHU2L_000876"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR1355848.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(11214..12008)
                     /gene="potC"
                     /locus_tag="ACHU2L_000877"
     CDS             complement(11214..12008)
                     /gene="potC"
                     /locus_tag="ACHU2L_000877"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR1355849.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(12005..12862)
                     /gene="potB"
                     /locus_tag="ACHU2L_000878"
     CDS             complement(12005..12862)
                     /gene="potB"
                     /locus_tag="ACHU2L_000878"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR1355850.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(12846..13982)
                     /gene="potA"
                     /locus_tag="ACHU2L_000879"
     CDS             complement(12846..13982)
                     /gene="potA"
                     /locus_tag="ACHU2L_000879"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR1355851.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            14232..15458
                     /gene="pepT"
                     /locus_tag="ACHU2L_000880"
     CDS             14232..15458
                     /gene="pepT"
                     /locus_tag="ACHU2L_000880"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR1355852.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(15507..16628)
                     /gene="roxA"
                     /locus_tag="ACHU2L_000881"
                     /gene_synonym="ycfD"
     CDS             complement(15507..16628)
                     /gene="roxA"
                     /locus_tag="ACHU2L_000881"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR1355853.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            16877..18106
                     /locus_tag="ACHU2L_000882"
     CDS             16877..18106
                     /locus_tag="ACHU2L_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1355854.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGEKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            18481..18669
                     /locus_tag="ACHU2L_000883"
     CDS             18481..18669
                     /locus_tag="ACHU2L_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1355855.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(18719..19048)
                     /locus_tag="ACHU2L_000884"
     CDS             complement(18719..19048)
                     /locus_tag="ACHU2L_000884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355856.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(19173..19352)
                     /locus_tag="ACHU2L_000885"
     CDS             complement(19173..19352)
                     /locus_tag="ACHU2L_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355857.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            19482..19682
                     /locus_tag="ACHU2L_000886"
     CDS             19482..19682
                     /locus_tag="ACHU2L_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR1355858.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19672..19893
                     /locus_tag="ACHU2L_000887"
     CDS             19672..19893
                     /locus_tag="ACHU2L_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355859.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            19886..20106
                     /locus_tag="ACHU2L_000888"
                     /pseudo
     CDS             19886..20106
                     /locus_tag="ACHU2L_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20108..20341
                     /locus_tag="ACHU2L_000889"
     CDS             20108..20341
                     /locus_tag="ACHU2L_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355860.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            20347..20646
                     /locus_tag="ACHU2L_000890"
     CDS             20347..20646
                     /locus_tag="ACHU2L_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR1355861.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            20643..22391
                     /locus_tag="ACHU2L_000891"
     CDS             20643..22391
                     /locus_tag="ACHU2L_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR1355862.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            22671..22949
                     /locus_tag="ACHU2L_000892"
     CDS             22671..22949
                     /locus_tag="ACHU2L_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355863.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            22946..23251
                     /locus_tag="ACHU2L_000893"
     CDS             22946..23251
                     /locus_tag="ACHU2L_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355864.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            23261..23335
                     /locus_tag="ACHU2L_000894"
                     /pseudo
     CDS             23261..23335
                     /locus_tag="ACHU2L_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23410..23523
                     /locus_tag="ACHU2L_000895"
     CDS             23410..23523
                     /locus_tag="ACHU2L_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1355865.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            23564..23788
                     /locus_tag="ACHU2L_000896"
     CDS             23564..23788
                     /locus_tag="ACHU2L_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355866.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            24089..25246
                     /locus_tag="ACHU2L_000897"
                     /pseudo
     CDS             24089..25246
                     /locus_tag="ACHU2L_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            25286..25858
                     /locus_tag="ACHU2L_000898"
     CDS             25286..25858
                     /locus_tag="ACHU2L_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR1355867.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            25860..27071
                     /locus_tag="ACHU2L_000899"
     CDS             25860..27071
                     /locus_tag="ACHU2L_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1355868.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            27068..27406
                     /locus_tag="ACHU2L_000900"
     CDS             27068..27406
                     /locus_tag="ACHU2L_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR1355869.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            27403..27699
                     /locus_tag="ACHU2L_000901"
     CDS             27403..27699
                     /locus_tag="ACHU2L_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR1355870.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            27699..28139
                     /locus_tag="ACHU2L_000902"
     CDS             27699..28139
                     /locus_tag="ACHU2L_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1355871.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            28123..28305
                     /locus_tag="ACHU2L_000903"
     CDS             28123..28305
                     /locus_tag="ACHU2L_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355872.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            28361..28687
                     /locus_tag="ACHU2L_000904"
     CDS             28361..28687
                     /locus_tag="ACHU2L_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR1355873.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            28671..30332
                     /locus_tag="ACHU2L_000905"
     CDS             28671..30332
                     /locus_tag="ACHU2L_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR1355874.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            30346..30627
                     /locus_tag="ACHU2L_000906"
     CDS             30346..30627
                     /locus_tag="ACHU2L_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355875.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(31424..32884)
                     /gene="phoQ"
                     /locus_tag="ACHU2L_000907"
     CDS             complement(31424..32884)
                     /gene="phoQ"
                     /locus_tag="ACHU2L_000907"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR1355876.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(32884..33555)
                     /gene="phoP"
                     /locus_tag="ACHU2L_000908"
     CDS             complement(32884..33555)
                     /gene="phoP"
                     /locus_tag="ACHU2L_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR1355877.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(33724..35094)
                     /gene="purB"
                     /locus_tag="ACHU2L_000909"
     CDS             complement(33724..35094)
                     /gene="purB"
                     /locus_tag="ACHU2L_000909"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR1355878.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(35098..35739)
                     /gene="hflD"
                     /locus_tag="ACHU2L_000910"
     CDS             complement(35098..35739)
                     /gene="hflD"
                     /locus_tag="ACHU2L_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR1355879.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(35775..36881)
                     /gene="mnmA"
                     /locus_tag="ACHU2L_000911"
                     /gene_synonym="trmU"
     CDS             complement(35775..36881)
                     /gene="mnmA"
                     /locus_tag="ACHU2L_000911"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR1355880.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(36935..37396)
                     /gene="nudJ"
                     /locus_tag="ACHU2L_000912"
     CDS             complement(36935..37396)
                     /gene="nudJ"
                     /locus_tag="ACHU2L_000912"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR1355881.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(37406..38059)
                     /gene="rluE"
                     /locus_tag="ACHU2L_000913"
     CDS             complement(37406..38059)
                     /gene="rluE"
                     /locus_tag="ACHU2L_000913"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR1355882.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            38231..39481
                     /gene="icd"
                     /locus_tag="ACHU2L_000914"
     CDS             38231..39481
                     /gene="icd"
                     /locus_tag="ACHU2L_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR1355883.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT        10063 a         9765 c        10456 g         9284 t
ORIGIN      
        1 cgaacgtaaa aaacgttagc cagcgagaaa aacgcgtttt gatcatcacc aaaaatacat
       61 aataaatata aggttattgt aataaacaga taagcctgaa ttatggatgg tgacagtgtc
      121 ggatagtgca ggaaagtgca aagaatttgt aaatgttgca gatgggggcg cagaaacgcc
      181 cccgatttac attaagcgat cgcgttctct tccagttcac gcataaactg gcgtacccat
      241 tcgatacgcg ttttccgctc actcaaatcc tgaataaatt tcaggcgcgt cggaccatca
      301 aggcggtaat gctgcggctg tttttgcagc aaaccaatca accaggccgg attaacgtga
      361 ttcttctcgg caaattcgat caccccgcct ttctcattac cttccagctt cctgatcccc
      421 agtttctgcg cttgctggcg cagacgggca atatccagca gggtacgcgc cggatctggc
      481 agcaggccga agcgatcgat aagctcgact ttgatctctt ccagttcgtt ttccgttttg
      541 gcgctggcaa tacgtttgta gaacgacaga cgcgtgttca cgtcagggat gaaatcatct
      601 ggcaatagcg acggcatccg cagctcgact tctgtttgct ggctggtgag atcttccagc
      661 gacggctcgc gtccggcttt cagtgcatcg acggcgtttt ccagcaactc catatacagc
      721 gagaaaccga tggtttccat tgagccgctt tgttcttcgc caagcagttc acccgcgccg
      781 cgaatctcca gatcgtgcgt tgccagcgca aaacctgccc cgagatcttc cagcgaggca
      841 atcgcttcaa gacgtttttg tgcatcggta gtcatcgctt ttggatgcgg cgtcagcagc
      901 catgcatatg cctgatgatg tgaacgtccg acgcgaccgc gtaactggtg cagctgcgcc
      961 agaccgaagt gatccgcacg ttcaatgata atggtgttgg ctgtcgggat gtcgatcccg
     1021 gtttcgataa tggtggtaca aaccagcacg ttgaaacgtt gatgatggaa atcattcatc
     1081 acccgttcca gttcgcgctc gcgcatctgc ccgtgaccga tggcaatccg tgcttcaggc
     1141 accagttctg ccagccgttc ggcggctttc tgaatgtttt ccacatcatt gtagagataa
     1201 taaacctgtc ctccgcgcaa aatttcacgc aggatcgcct cccggaccac caggctgtca
     1261 tactcacgga caaaggtttt aactgccaga cgacgggcgg gcggcgtggc gataatcgac
     1321 aggtcacgca ttccgctcat tgccatattc agcgtacgtg ggatcggcgt tgcagtaagc
     1381 gtcagaatat ccacgttcgc gcgcatcgct ttaatgcgct ctttatgacg caccccgaag
     1441 cggtgttctt catcgacaat cagcaggcct aaatctttaa acttgacgtc actttgcagc
     1501 agtttgtgcg taccgatcag aatatcgatt ttcccttccg ccacttccgc aaggatttgc
     1561 gtctgctctt tggcgctgcg gaaacgggag atcatttcga tacgtaccgg ccagttggcg
     1621 aaacggtcgc ggaagttgtc gtaatgctgc tgcgcaagaa gggtggtagg caccaacacc
     1681 gctacctgct tgtggttatc tactgccagg aaagcggcgc gcatcgccac ttctgtttta
     1741 ccaaagccaa catcgccgca caccagacga tccattgcca gcggctgaca catgtcgcta
     1801 agtaccgcat taatggcctg cgcctgatcc ggcgtggttt caaacgggaa gctgtcgcag
     1861 aataactgat actgctcacg atcgtgttta aacgcgaagc cctctttggc ggcgcgttgc
     1921 gcgtagatat ccagcaattc cgccgccaca tcacgcactt tttccgccgc tttctgccgc
     1981 gcgcgtgacc acgcatcgcc gccaagttta tgcagcgggg cgttttcttc cgcgccacct
     2041 gcgtaacggc taatcagatg cagtgacgac accggaacat acagtttggc gtcgttggca
     2101 taggtgagca tcaggtactc gccagtaatg ccgccagctt ccagcgtggt cattccggca
     2161 tagcgaccga cgccgtgctc cagatggacc accggctgac caatatgcag ctccgcaagg
     2221 ttacggatca gtgtatcggg gttgatggtg cggcgagaat cctgacgacg gcgggcaaca
     2281 cgttcaccga gcagatcgct ttcgcagatc agcgccagat tacgcgcctt atcgacaaaa
     2341 ccatgttcgg cagcgccaat catcagataa cgcccacggt cgctggcttc atcaagacgc
     2401 ataatgcgtt gcggagcaat tttaattcgt gcgagcagtt cacccagcgc ttcacggcga
     2461 ccttcacttt ctaccgagaa caccaccgga ccgtcgaaag tctcgaggaa cttacgcagc
     2521 gcatccagcg gcgctttttg ttgcgcctga acggccaggt ctggcagttt ctggaaacct
     2581 aaattggcat tcgcggcttt tgtcggtaaa tgttcagttt ttagctgcac tcggggccag
     2641 tttttcagct ctgagaagag ctcgtccacc cgcagccaga gcgattgtgg tggcaacagc
     2701 gggcgcatcg gatcgacgcc gcgattctca aaacgcgcca gcgtgtcagc ctggaaacgt
     2761 tcggcactgg tttccagatc gccagtattc acgagcaagg tattggcagg gaaataactg
     2821 aacagcggtg gcagtggttc gctgaagaac aatggctgcc agtactcgat cccagcaggt
     2881 aatgtgcctt tactcacttg ctggtaaata tgttccggat cgcgcttcac ttcgaaggta
     2941 tcgcgccact ggctgcggaa cagttcaatt gccgctttat cggtcggaaa ttcgtgcgcg
     3001 ggcagcagat tgatcgcttc tacttcctcc agcgtgcgct ggctgtcgac gtcaaacacc
     3061 cgcaggctgt cgatttcatc atcaaagaaa tcaagacgat aaggcagctc actccccatc
     3121 gggaagagat ccagcaacgc gccgcgcgtg gcgtattcgc cgtgctccat cacctggtca
     3181 acatggcgat aaccggcgct gtccagttgg gttcgtaatg catctcgtga caggcgctga
     3241 ccttttttca tcaccagcgc atgaccgtgg agaaaactgt gtgggcaaac gcgctgcata
     3301 agcgtattca ccggaacaat cagtatgcca cgctgcatcg tcggtagctg gtaaagggtg
     3361 gaaaggcgcg aggagataat gtcctgatga ggcgaaaaac tgtcgtaggg aagagtttcc
     3421 cagtccgcca gattcatcac catctgatcg gtgaactggc tgatttcatc atgcaaacgc
     3481 agagcatttt gcatatctgg tgcaatgagt accaccggac cggcgtgacg ttcggcaatt
     3541 tccgctacca gcgttgcaca ggctgcgccg gttaactcgc ccagcagacg ctgctcaccc
     3601 gctttgacgg gcagcgtata acgatattgt tcaggcataa cggttgtcag attctcgtta
     3661 ggatatgcct caacatatgg gggcatatct ctgataagca atgctgttca ttatccgtaa
     3721 tcgacatggt ttagcaaatt gaatcgcccc ggctggggtg atggtttaac gagagtaact
     3781 gacggcagcg ggcagtgaag gcggggagaa caacagatcg ctgaatgcac gctgtgaaag
     3841 ttttcttacc agcaagcccg caacagtaca gatgaacaaa ctgacaaagg gataaaccag
     3901 caggagtgtc agttcgactt gcggcgacca gcgtgctgcg ttcatttgcg caagcagagt
     3961 taagctgaat atttcaacca gaatgcgatg ggtggtgtag atagcaatgg tgttggagcc
     4021 aatcacattc agcaggctgg tggagcgcat accgaatcgt tgctcgtact gataaaacag
     4081 cttcatgatc accacaatcg ataccagcga caacagcagc gagatagtaa acaaccaggc
     4141 accgaccgcc agaacggtaa gcaaagaagc catcagcaga tggcggcgca acggcacctc
     4201 tttaacacag gtcattattg ttgcgccaaa ccatgcgcca aggctgtaat aaggcaaatt
     4261 gcgtatcaca ctgttcattc cccaccacgg cgtgggaacg aaattaaccg ccacactcag
     4321 cagtacaaac aaggcgaata gtggcagcgc cagacggcta aaaattttac ataccacgaa
     4381 atagacaatt aacgcataca gataccacaa gctggtgctg gcggtgatca tcccgtgcag
     4441 gaactcaccg gtggaatcgg cataagcggc attggaggca ttgcttaaat cgcgctcagg
     4501 tgccagccac tggttcagcg cacttaacgc cagccactgc accacgcccc aaagcgccag
     4561 cacccagaag atgttccaga tccgtttatc gagacaattt ccccacggta cgctgtcgat
     4621 atagcggcga atcaaatagc cggatataaa gaaaaaaacc ggcatacgaa agggggcaag
     4681 gtaaagattg aaatagatcc agcatttgct caggacttcc gataacggat gctgaaaagt
     4741 ggtcatatgc ggataaaagg taatgaccga gtgataaatc accaccagac aaatacataa
     4801 ccctttgatc tggttaatcc atagctcttt ttgtttcatc agaacaacac gctctttttt
     4861 aatttacatg cggttgatcc tgctggttat gacccgaggt gtaatgggta tctgtctgta
     4921 ttggatgatt tttcagatta agatcaggcg gcaagattga tgataaaata tggcaattta
     4981 gccgattgat ttacgggggc ttttcagatt agccctgacg atcacttaca gttcagacgt
     5041 ttacccatct tgctttcgct tatatactcg tgtctttgct acagcaacca gacggatttc
     5101 atgtaccaac ctgtcgctct atttattggc ctgcgttaca tgcgtgggcg tgcagtggat
     5161 cgcttcggtc gtttcgtctc ctggctttct accatcggca ttaccctcgg ggtgatggcg
     5221 ctggtcacag tattgtcagt gatgaacggc tttgagcgcg agctgcaaaa caacatcctt
     5281 ggcctgatgc cacaggcaat tctctcttct gagcatggct ctcttaaccc gcagcaactc
     5341 ccggaaacgg cagtcaaact ggacggcgtt aatcgcgtcg cacctattac taccggtgat
     5401 gtggtactgc aaagcgcgcg cagcgtggcg gtcggggtga tgctcggtat cgacccggcg
     5461 caaaaagatc cactaacgcc gtatctggtc aatgtgaaac aaactgacct cgagccgggg
     5521 aaatataatg tcatcctcgg cgaacaactt gcctcacagt tgggcgtcaa tcgcggtgat
     5581 caaatccgcg tgatggtgcc atctgccagc cagttcacgc cgatggggcg tattccaagt
     5641 cagcgcctgt tcaatgtgat tggcactttc gccgccaaca gtgaagtcga tggctatgaa
     5701 atgctggtga atattgagga tgcctcgcgt ctgatgcgtt atccggcagc caatattacc
     5761 ggctggcgtt tgtggctgga tgagccgctg agagttgact ctttaagtca gcaaaaactg
     5821 cctgaaggca gcaaatggca ggactggcgt gaccgtaaag gcgagctgtt ccaggccgta
     5881 cgcatggaaa aaaatatgat gggcttactg ctgagcctga ttgtcgctgt tgcggcgttt
     5941 aacattatta cctcgctggg gctgatggtg atggagaagc agggcgaagt cgcgatcctg
     6001 caaacgcaag gcttaactcc gcgacaaatc atgatggtct ttatggtgca aggggctagc
     6061 gccgggatta tcggtgcaat cctcggagcg gcgcttggcg cactgcttgc cagccagtta
     6121 aataatctga tgccgataat cggcgtcctg cttgatggcg cggcgctgcc ggtggctatc
     6181 gaacctttac aggtcattgt tattgcgctg gtggcgatgg ctatcgcgct gctgtctacg
     6241 ctttaccctt catggcgcgc tgccgccaca caacccgctg aggctttacg ttatgaataa
     6301 gatcctgttg caatgcgaca acctgtgcaa acgctatcag gaaggcagtg tgcaaaccga
     6361 tgtgctgctc aatgtcagtt tcagcgtggg cgaaggtgaa atgatggcaa ttgtcggtag
     6421 ctctggttcc ggtaaaagta ccttgctgca cctgttaggc gggctggata cgccaacctc
     6481 cggcgatgtg atctttaacg gtcagccgat gagcaaactg tcttcagcgg cgaaagcaga
     6541 actgcgcaac cagaagctgg gctttattta tcagtttcac cacctgctgc cggattttac
     6601 tgccctggaa aacgtggcta tgccactgct gattggcaag aaaaagcccg ctgaaatcaa
     6661 cagctgtgca ctggagatgt taaaagcggt ggggctggag catcgtgcga atcaccgccc
     6721 atctgaactt tctggcggcg aacgccagcg tgtagcgatt gcccgtgcgc tggtcaataa
     6781 tccgcgtctg gtactggcgg atgaacctac cggtaacctc gatgcgcgta acgccgacag
     6841 catcttccag ttgcttgggg agttgaatcg cttgcagggc accgctttcc tggtggtcac
     6901 tcacgacctg caactggcga aacgtatgag ccgccaactg gaaatgcgtg atggacgtct
     6961 gacggcggaa ctgagcctga tgggggcgga gtaatggcga tgcctttatc gttattgatt
     7021 ggcctgcgtt ttagtcgcgg acggcgacgc ggcggcatgg tgtcgctaat ctctgtcatt
     7081 tctaccattg gcattgccct cggcgtggcg gtattgatcg tcggcttaag cgcgatgaac
     7141 ggctttgaac gcgaactgaa taaccgcatt ctggcggtgg tgccgcatgg cgaaatcgaa
     7201 gcggtaaatc agccatggac taactggcag gaagcactgg ataacgtgca gaaagtgccg
     7261 ggtattgccg ccgctgcgcc gtatatcaat ttcaccggtc tggtggaaag tggagcgaat
     7321 ctgcgcgcaa tccaggtgaa gggcgttaac ccacaacagg aacagcgtct gagcgcatta
     7381 ccctcgtttg ttcaggggga tgcgtggcgc aattttaaag cgggcgaaca gcaaattatc
     7441 atcggcaaag gcgtggcgga tgcgctgaaa gtgaagcagg gcgattgggt gtcgatcatg
     7501 atccccaact cgaatcccga gcataaactg atgcagccaa aacgtgtgcg tttgcacatt
     7561 gccggtattt tgcagttgag tggtcaactc gatcacagtt ttgccatgat cccgctggcg
     7621 gatgcccaac aatatcttga tatgggttcc agcgtgtcag gtattgccct taaaatgacg
     7681 gatgttttca acgccaataa gctggtacgc gatgcgggtg aagtgaccaa cagctatgtt
     7741 tatattaaaa gttggattgg tacttacggc tatatgtatc gcgatatcca gatgatccgc
     7801 gccattatgt atctggcgat ggtactggtg attggcgtgg cctgtttcaa catcgtctcc
     7861 accttagtga tggcggtgaa agacaagagt ggcgatatcg cagtattaag aacgctgggg
     7921 gcgaaagatg gtttaattcg cgccatcttt gtctggtatg gattgctggc agggctgttc
     7981 ggcagcctgt gtggggtggt tatcggcgta gttgtttcac tgcaacttac cccgattatt
     8041 gagtggattg aaaagctgat cggtcatcag ttcctctcca gcgatattta ttttattgac
     8101 ttcctgccat cggaattgca ctggctggac gtcttctacg tactggtcac agcattgttg
     8161 ctgagtcttt tggcaagttg gtatccggcg cggcgcgcca gtaatattga ccctgcgcga
     8221 gtccttagcg gccagtaaag gcagtacatt aaaacaagga gcggcaatgt attacgggtt
     8281 tgatattggt ggaacaaaaa ttgcgcttgg cgtgtttgat agcggtcggc agttgcagtg
     8341 ggaaaagcgg gtgccgacac cgcgtgacag ctatgacgca tttttagatg cagtgtgtga
     8401 gctggtagct gaagctgacc aacgttttgg ctgtaaaggt tctgtcggca tcggtattcc
     8461 gggaatgccg gaaacagaag atggtaagct gtatgccgct aatgtccccg ctgccagcgg
     8521 caaaccgctg cgtgccgacc tgagcgcacg tcttgatcgc gatgtacgcc ttgataacga
     8581 tgccaactgt tttgcccttt cagaagcctg ggatgatgaa tttacgcaat atccactggt
     8641 gatggggtta attctcggca ccggcgttgg cggcgggctg gttttcaacg gcaaaccgat
     8701 tactggtaaa agctacatta ccggcgagtt tggccatatg cgtctgccgg ttgatgcgtt
     8761 aaccatgatg gggttggatt tcccgttacg ccgctgcggc tgtggtcagc atggctgcat
     8821 tgaaaattat ctgtctggtc gcggttttgc gtggctgtat caacactatt atcatcaacc
     8881 gttgcaggct cccgaaatta ttgcgcttta tgatcaaggc gatgagcagg caagggcgca
     8941 cgttgagcgt tatctggatt tattagcggt ttctctggga aatatcctga ccattgttga
     9001 ccctgacctg gtcgtcattg gtggtggctt atcgaatttc ccggcaatca caacgcaact
     9061 ggcggacagg ttgccttgtc atctcttacc tgtagctcgt gttccacgca ttgaacgcgc
     9121 gcgccacggt gatgcgggag gaatgcgtgg tgcggccttc ctacatctaa ccgattaaac
     9181 aacagaggtt gctatgctgt cgcgtcgggg tcatcggtta agtcgttttc gtaaaaataa
     9241 acgtcgcctg cgcgagcgtt tgcgtcagcg tatttttttc agagataaag tggtgccgga
     9301 agcaatggaa aaaccaagag tactcgtact gacaggggca ggaatttctg cggaatcagg
     9361 tattcgtacc tttcgcgccg cagatggcct gtgggaagaa catcgggttg aagatgtggc
     9421 aacgccggaa ggtttcgatc gcgatcctga actggtgcaa gcgttttata acgcccgtcg
     9481 tcgacagctg cagcagccag aaattcagcc taacgccgcg catcttgcgc tggctaaact
     9541 gcaagacgct ctcggcgatc gctttttgct ggtaacgcag aatatagaca acctgcatga
     9601 acgcgcaggt aataccaatg tcattcatat gcatggggaa ttgctgaaag ttcgttgttc
     9661 gcaaagtggt caggttctcg actggaccgg agacgttacg ccagaagata aatgccattg
     9721 ttgccagttc ccggcaccct tgcgcccgca cgtcgtttgg tttggcgaaa tgccactcgg
     9781 catggatgaa atttatatgg cgttgtcgat ggcggatgtc tttattgcca ttggtacttc
     9841 cgggcatgtc tatccggcgg ctgggtttgt acacgaagcc aaactacatg gtgctcacac
     9901 cgttgaactg aatcttgaac cgagtcaggt tggtaatgaa tttgccgaga aatattacgg
     9961 cccggcaagc caggtggtgc cagaatttgt tgaaaagttg ctggagggat tataaagcag
    10021 gaagcattgc ctgatgcgct acgcttatca ggcctacaag cggaccacaa taccgacgaa
    10081 agagcgggat aaaactttac tgtcctgact cattagccac atccttgcta actaaaaaac
    10141 gggcggtaat accaccgccc gcttgctgaa ttaacgtcct gctttcagct tctgataata
    10201 ctcttcataa atgctgctgg ctgcgccaac gtcattctgc cattcgccat ttttaatggt
    10261 ttcagcatcc gggtagagcg tcttatcgtt tgccacttct ggacttaaca gcttacgcgc
    10321 cgcaaggttt ggcgttggat aaccgatagt ttcagcaacc tgttttgcca catccgggcg
    10381 cagcaggaag ttgatcaatt tcagcgcgcc ttctttgttt ttggcatttg ccgggatcgc
    10441 caggctgtcc atccagaata tgccgccttc tttcggccac accacgtcaa taggcgtacc
    10501 cgcctggcgt gcaacgaaag cagaaccgtt ccagatcatg ccgaggttaa cttcgccttc
    10561 catgtacggg ttagccgggt tatcggagtt aaacgctgcg acgtttggca tcagtttttt
    10621 cagctcgtta tatgcagctt caatctcttt cggatcggtg gtgttaccgg agtagcccag
    10681 cttacgcagc gccatctgga acacttcacg ggcatcgtcg gtcaacagca ggctgccttt
    10741 gtactctggc ttccacagat cggcccagct ggtgacagat ttcggatcca ccgcatcacc
    10801 gttaacacca atcgccgtcg caccccagat atacggaatg gagtagtcgt tattcgggtc
    10861 aaaaggcttg ttgagcatgt ctggatcgag attgctgaag ttcgttaact tcgacttgtc
    10921 gatcttctgg atcatcccct ctttacgcat tttatcgacg taataggttg aaggaaccac
    10981 caggtcatag gcaccgtctt tgtacgtttt cagcttggcg tacatggttt cgttcgactc
    11041 gtaagtcgaa tagataacct taataccggt ttctttggtg aactgttcaa gcagtcctgg
    11101 cggcacgtac tcggtccagt tgtagaaata cagcgtgttg ttgtcatcgg cgtgagcggc
    11161 gctcatgccc agtgccagag cacccgccgc gagcaggtgg cgtgaccatt ttttcattta
    11221 acgtcccctg tgttaccttt cgttttatca cgagcaataa gctggctggc aataaccatc
    11281 accagcgaca gcaccagtaa tatggttgcc agcgcgttaa cttccggcga tacgccgact
    11341 ttgaccatcg aataaatttt taacggcaga atttcataac ttggtccggt gacgaatgaa
    11401 gaaactacca catcgtccat cgacagggta aagcttaaca cccagcccgc cgccaccgct
    11461 ggcattgcca gtggcagaat gatttttcgc agaatggtaa attcgctggc accgagatct
    11521 ttcgccgctt ccagcatccg cacgtcaaaa cctttcaggc gcgaatacac cgtcaccacc
    11581 acaaatggca ggcagaaggt gatatgcgag aacagcagcg accagaagcc aagctgaata
    11641 cccagcagca taaacagcac cagcagcgaa attgccatca cgatatctgg tgacatcatt
    11701 accacaaaca gcattccgct aacgaacggc ttaccgcgaa agcgataacg gtacagcgca
    11761 actgccgtca gtgaaccgat aagcgtagca aacgtcgccg aaaacaccgc cattgttagt
    11821 gaatgctgcg ctgcctgtaa caggctgtcg ttgttcatca gcaggctata ccatttggtg
    11881 gtaaaaccct gccagttgat gccaaagcgc gagctgttaa aggagttcac aatcaaaata
    11941 atgattggga tatacaggta cgcgtagata gcggtcataa aaccgccgcg aagcagtcga
    12001 ccgatcattc gagttccacc ttcttattca gcaaacgaga agcgcgccag taaaccagca
    12061 acatcaggcc cattacgata gtcagcgtaa tgctggtggc tgcaccaaac ggccagtcac
    12121 gaatattgag gaactgtacc ttgatgacgt taccgatcag caggtttttc gcaccgccca
    12181 tcaggtcgga cacatagaac aggcccatcg ctggcagcat caccagcaga catccggcaa
    12241 taatccccgg catcgtcagt ggaataatga tgcggataaa agtctgtaac ttgctggcac
    12301 cgagatcgcg cgccgcctca agcaacggct tatccagttt ttcgatactg gagtacagcg
    12361 gcatcaccat aaacggcagc agaatgtaaa ccagaccgat aatcaccgca ctgggcgtga
    12421 acatgatacg gattggtgtg tcgataacgc ccagccagag caaaaactcg ttgagatagc
    12481 ctttggtgct gaggaaaatt ttcagcccgt agatacgaat caatgagttg gtccagaacg
    12541 gaacaatcag cagaaacagc agcagcggac gcaccttgtg tggcagcttc gccagaaacc
    12601 aggcaaatgg atagcccagc accaggcagg cgagggtggc gatcagcgcc atattcagcg
    12661 agtgcaatag cacttcaaaa tagagcggat cgagcagacg cgtgtagtta tccagtgtaa
    12721 agaccatttt gacgaaactg gcgtcgtcgc gggtcaaaaa gctggtgcca atgatcatca
    12781 ggttgggcag aaagacaaac aacacaagcc aaccgacaat agtgacaatc actacattct
    12841 ggaacttact tgtgttcttc atcagccagt acgacctccc agctttctac ccaattaatg
    12901 gccatttttt ggtcgagaga gtggtcaaag tcaggatcgt cttcattgaa gaattcgctg
    12961 accatcacca tcttgccatt ttccagttca acaaccgact ccagcgtcat gcctttgtag
    13021 ttacgctcac gaacgtaacc aatcagccct tcagcgtggt tgtcgtcgtt aatctcttca
    13081 acacgtaagt cttccgggcg cagcagaaca tgcagttttt gccccggttc aacggcgaag
    13141 ttaacgtaga tattacattc gcggccttca acgttggcgc gtacgcgctg ctcgtcgagt
    13201 cgctcgatca ccgtggcgtt aaacatatta atttcgccaa tgaacccggc aacaaacagg
    13261 tttttcggct cttcgtagat ttcacgcggc gtgccgtctt gctcaatgcg accatcgcgc
    13321 atcaccacaa tcctgtccga catggtgagt gcttcttcct ggtcgtgagt cacgaaaacg
    13381 aatgtaatgc caagcttacg ctgtaacgct ttcagctcgt tctgcatttg cttacgcagt
    13441 ttgtaatcca gcgctgagag cgactcatcc agcaacaaca gacgaggctt gttaaccacc
    13501 gctcgagcaa tggcgacgcg ttgctgttga ccaccagaga gctgatgcgg tttacgttga
    13561 gcgaaggttt ccagctgcac catccgcagg gcttccatca cgcggggcgt aatttcagca
    13621 gcgggggttt tttgcatgcg caacccaaag gccacatttt cgaacacggt catgtggggg
    13681 aaaagtgcat agctttggaa aacagtgttc acatagcggt tttccgccgg aacgtgggtg
    13741 atgtcctcgt tatccagcat gatgcgtccg gaatcaacag tttccagacc tgcaatcagg
    13801 cgaagaacgg ttgttttacc gcagccagaa gggccaagca gcgtgaggaa ctcgccattg
    13861 ttgatagtca gatccagctg gggaatgacc tctttaccat caaagcattt gcgaattccc
    13921 gccaattgca ccagcggtga aagcgaactc ggttgtttat tcaatttttt actctgtccc
    13981 atgtaaacgc aacggatggc ttaccgatgc ggggtttgtg gttaaccacc ttggtgactc
    14041 ctaatgaggg cggtaattct acggcaaacc gcttgaatcg ccaatctttg ttgtgaatta
    14101 ctggcttagc tttatattca ttaaggtaat gctgataaat attcccgctt gcaggggtaa
    14161 aagtgacctg acgcaatatt tgtcttttct tgcttcttaa taatgttgtc acaaaaagtg
    14221 agggtgacta catggataaa ctacttgagc gatttttgaa ctacgtgtct ctggataccc
    14281 aatcaaaagc aggggtgaga caggttccca gcacggaagg ccaatggaag ttattgcatc
    14341 tgctgaaaga gcagctcgaa gaaatggggc ttatcaatgt gaccttaagt gagaagggca
    14401 ctttgatggc gacgttaccg gctaacgtcc ctggcgatat cccggcgatt ggctttattt
    14461 ctcatgtgga tacctcaccg gattgcagcg gcaaaaatgt taatccgcaa attgttgaaa
    14521 actatcgcgg tggcgatatt gcgctgggta tcggcgatga agttttatca ccggttatgt
    14581 tcccggtgct gcatcagcta ctgggtcaga cgttgattac caccgatggt aaaaccttgt
    14641 taggtgctga tgacaaagca ggtattgcag aaatcatgac cgcgctggcg gtattgcaac
    14701 agaaaaacat tccgcatggt gatattcgcg tcgcctttac cccggatgaa gaagtgggca
    14761 aaggggcgaa acattttgat gttgatgcct tcgatgcccg ctgggcttac actgttgacg
    14821 gtggtggcgt aggcgaactg gagtttgaaa acttcaacgc cgcatcggtc aatataaaaa
    14881 ttgtcggtaa caatgttcat ccgggcacgg cgaaaggagt gatggtaaat gcgctgtcgc
    14941 tggcggcacg tattcatgcg gaagttccgg cggatgaaag cccggaaatg acagaaggct
    15001 atgaaggttt ctatcacctg gcgagcatga aaggcaccgt tgaacgggcc gatatgcact
    15061 acatcatccg tgatttcgac cgtaaacagt ttgaagcgcg taaacgtaaa atgatggaga
    15121 tcgccaaaaa agtgggcaaa gggttacatc ctgattgcta cattgaattg gtgattgaag
    15181 acagttacta caatatgcgc gagaaagtgg ttgagcatcc gcatattctc gatatcgccc
    15241 agcaggcgat gcgtgactgc gatattgaac cggaactgaa accgatccgc ggcggtaccg
    15301 acggcgcgca gttgtcgttt atgggattac cttgcccgaa cctgttcact ggcggttaca
    15361 actatcatgg taagcatgag tttgtgattc tggaaggtat ggaaaaagcg gtgcaggtga
    15421 tcgtccgtat tgccgagtta acggcgcaac ggaagtaagc gaaaagggat gcggcatgtg
    15481 atgctgcatc cggcttaaat ccaaacttac ccttcgaaga accaataccc gctattgacc
    15541 agcgccgcga gcatcgcgag gaatgacgga tcttccagcg catcgccaaa attctccgca
    15601 gtcagcgcaa tgttgctggc gagtgcatcc agtgccggac ggtgcgggga atcgatcttc
    15661 tcaccattgg catacacgtc gtcgccaatg cgcaatacgc gcagaccacc caggcgcacc
    15721 agcacatcac cttgtttcag cgcatcgtag atttcatctg gctgataagg cggttccggc
    15781 ggcgcgatat ccagttcatg acgtgactgg gatataaact cgccaaacca ttgcttaaag
    15841 tgttccggct ggttgatcaa ttcgagcatc atctcacgca gtttatccat ctcttgcggc
    15901 agaacatccg caggatgagc acgaggtgga acatccggat cgctgtagta gttgccgccc
    15961 agttcacgtt gcagcacata atcggcaaat ccgctaatta attcccgcgt atttggcgcg
    16021 cgaaaaccca cggaatagtt catcgcattt tccagcgcgt agccttcatg cgggaatcct
    16081 ggcggaatat aaagaatatc accaggctcc agctcttcat cgatgatggc ttcgaacgga
    16141 tcgacctgta acagatccgg atgtgggcag tgctgtttca tttgcagctt ttcgcccact
    16201 cgccagcgac gacgtccggt accctgaatg ataaacacgt cgtactgatc gagatgcggg
    16261 ccgacgccgc cgccaggtac agaaaaagaa atcatcagat catcaatacg ccagtccggt
    16321 agttcacgga acggtcgcat cagcgcggcg gtcggctcat gccagtggtt tactgcctgt
    16381 accagtaatg accagttggt ttcaccgaga tgatcgtagc tttcgaacgg gccgtggctg
    16441 acctgccatt tgccatcctg gtgactgacc agtcgactgt caacttcgct ttccatcgcc
    16501 agacccgcca actcgtctgg agagatcggg tcaataaaat tattaaagcc gcgttttaac
    16561 accaccgggc gtttctgcca gtgacgttca agaaaatcgg gccagttaag agtgagttgg
    16621 tattccatgt taagcatcca caggctggta tctgcaaccg attataacgg atgcttaacg
    16681 taatgcgtga agtatggaca tattttatca attagtgttt tttgccgttt cataatgttt
    16741 cagattgttc attatgtttt tgattataaa cagatttgtg attttccttg ttccagcttg
    16801 tttcacgttg tagccctttg tgcaacgatc ggtgtagtta atggtgtagt taattgtgat
    16861 gaccggaatc aaaattatga gcagagcact taacaaactg agcgatacac agctgaggaa
    16921 aatcaacggc acacccgccc aaaaaacagc ctttcttaat gacggtggaa acctgagcgt
    16981 caggcattca accagtggcc ttttaacctg gtatttcact tacagggccg gaacgggaag
    17041 gggggcacca ccggaacgca ttaagctggg aaattatcct gatctgagcc tgaaatcagc
    17101 cagggaaaaa gccgcccagt gtcgcgcatg gctggcagag gggaaaaatc cacgtcatga
    17161 gcttaattac accgtacagg aagcgttaaa gccggtaacg gttggcgatg cgctcaccta
    17221 ctggcttgag tcgtacgcaa aggaaaaccg cgtggattat gccgccctga aaaagcgcct
    17281 taataatcac gtaatacagc acattggtgc tatgccgctg gataaatgcg agctacggca
    17341 ctggctggcc tgttttgacc aggtggcaaa gcgaacgcct gttactgccg gattcttgct
    17401 acagacgtgc aaacaggcgc ttaagttctg ccggaggcgg cgctatgcaa tcagcaacgt
    17461 tcttgatgat atgagtgtgg cggacgttgg ggaaaaaccg gatataagcg agcgtgtctt
    17521 aagcaccaaa gaactgggcg aattattgca ggcactggac aaaaaaatat tctcccccta
    17581 ctacatcgcg ttaatccgcc tcctgattgt gttcggatgc cggacggtag aactgaggtt
    17641 atcggagatc agcgagtggg attttaccga aatgctctgg accgtgccga aagaacacag
    17701 caaaacgaag gtggcaatat tccggcccat accggaagca atactgccgt tcgtcacgca
    17761 gctggtggag cagaacaggc acacgggctt attgctgggg gaagtgaaac aggaaacaag
    17821 cgtgtcgcag tacggcagat tagcgcacag gaggctaaat caccctcact ggtcactaca
    17881 tgacatccgg cgcaccttta caacgatgct gaacgattta ggcgtggatc ctcacgtcgt
    17941 ggagcagctt acaggccacc agatgccagg aatgcagcga gtttataatc attcccgtta
    18001 tctggatgct aaacgcaatg cgctggatat gtggacggag cggttaggga tactggcggg
    18061 aacacatgaa aacgtaacca cgctaccagt agccagaaga aaataatttt tttcgtgttt
    18121 tttcagtatg cgcatactgg atatgcgaac agatacaacg tgcaacaatg agcaacaatg
    18181 cggaacaact acgaacaaga ggtgaaaaag tgtacagatt tataagtagc tgatttttaa
    18241 tgtgttactg attttttata cactagcgaa tcactcttta aatagcgaga aaaaaggcat
    18301 gaaacaatat tttatgcctt tgtattaaca tgaatttaaa ggattttatc ctgattattc
    18361 ctctttctgt aaaccatacg gcctcaacat ttgccaactc tgaatcatcc tgacacaatc
    18421 agacacatcc caacgcaata gaacacgaaa taactcttta agaaacgaaa gggggcaata
    18481 gtgttaagca ctgatcggtt tatacgtgaa aaagaatgcg aaaagctaac aggccttagc
    18541 cgctcatgcc gctaccgcct ggaaaaggcc ggacaattcc catcacgtcg taaacttggc
    18601 ggtcgttccg ttggctggtc tttatccgag gttctggcct ggaaggatag ctgcaaggca
    18661 gttcattaat cacgctggcg gcacgcagcc gccacacatc aatcatctga acacagagct
    18721 ataaccatga agattgaata tacgccagaa cgtgggcggg gattcgttcg ccctggtgaa
    18781 actggaaaac cacaaaattg gggtttttca ggcataaaaa aagcggcccc gaaatggagc
    18841 cgcctttctg aacagataac ccgctgcgcc gtttgtgtgt gtgatcccaa acataagcac
    18901 ggggatgata gccgctatca ggctggtggg caatgcaatc agtctggttc agtgcgttgc
    18961 cataactgcg aagttctctc ctttgatgag tattctttaa ggtacttctc aagggcaaaa
    19021 gaacacggcg cgaatcgttc tgattcatgc tctttctttc tgcgtcgtct ttttcgtgca
    19081 ggtgatgttt tttcggttga ttcttcgttg gtcattgtgt gcgcctgtaa agcaatgcgc
    19141 cgtagtacct cacaccacgg cgctgatagt ttttatcctt tgggttctat gccgcgttgc
    19201 tgtaattctt tgcggattat tcgctttatc caggcagcaa gagaatcatc gccgtcctgt
    19261 tgttgtgcct tttccatctt ttcccgtaag tcaggatcta aacggaactg gaacggagga
    19321 ttaccacgcc tttcgttttt gtgtgttgac acgttaatta cacctcaggt aatgtattta
    19381 tgtgtagtga cacattacac acaatcaaca caaaaagcaa agccccgcac tgtaggagca
    19441 gtaacggggc ttctaaccaa caacgtaaac taggagccgt tatggttgcc gtaaatcata
    19501 taccacacct tgtacacaca caaacggcct ttgtgtggcg ttttctggca ctgagtgccg
    19561 gagaatccca aatcatccac gtaaccgctt ggacggaacg cgaagcgcgt agccgttgcc
    19621 cgtccggttg tgttgctgta ttcgccgccc gtattcgtca gggggaaacc tatgcacaat
    19681 aaaaccacac cgaacgcagc agccgccgca ctcactacgc tgatgcacgc gcttattgat
    19741 attgaatgca cggcagagct tgcgcaggga gaagaacaga aagaccggac acagttcgcc
    19801 ctggaatgta tccgatacat cgcaacgcgg tcactgaatg acgctaaaaa tattcttgtt
    19861 gccgattgtg aaaatggggg gggttatgcg tgatgatcgt tttaattccc tgaaacagga
    19921 attttccggc gttcctgatg atgcggctga tgcgctttcg tcaatgccag aacttattag
    19981 agcggctttt ttcttacttt ccacgagaga atataaatca acgggcttga tgtactgaat
    20041 atcgccgccg attatgctga tttcgtgaca gaggttattt taagaaaaac gacggacggg
    20101 gattaatatg cgtgatattt accacgaaac aatagaccgc gcatttcttg cactttctca
    20161 cagtgaaaac atgctggaaa tattgcgcat atggcttgaa acacttggcg acaatgaacg
    20221 cgacaaacaa aaatcaagaa ttgccacggc attaataacg cttcttgagc ctgtaataat
    20281 ggaactgcaa gaaatagatc tattgcacga cagatataaa gaacaacaca ccggagaata
    20341 aaaataatga aacttaaata ttctggctta actgccagtg gcaacactca ccctaaattt
    20401 acgcgcggtg atatttaccg cgaccagtac ggcggcatgg taacgattaa aggcgtggca
    20461 gaacggcgca tcacctaccg ccgtgaaggt tacgaatatg attgcgtgat gcctgtttat
    20521 caattccggc gtgatttcac gctggtacag gcagcgcccc gcagtaaacc aaccagcagg
    20581 gagaaagcac gcgccaatat tcaggaaata aaaaagatgc ttaacgtatt caggggtaaa
    20641 aaatgaaact ggcaccgaac gtaaaaaaac agccacgcgg aataaaacac aaagacacag
    20701 aggtaattat ctttgcgggt agtgatgcct ggtcgcacgc gaaacaatgg caggagcagg
    20761 atggccccgc atccggcgat aatgtgccgc ctgtgtggct tgggccaaat cagcttgccg
    20821 aacttgatgc actgaaaatt gttccggatg gaaaaaaacg cgtaaggctg taccaggccg
    20881 gagaactgga tttggtggag accaaaaaga ttggtcagaa gctggcggcg gcagatattc
    20941 aggacgcaaa tttttacccc gaaggaatgc acgtccagaa gtgtgaaaac tggcggcgct
    21001 atctgaatgc tgagcgtgaa aatattgccg cagggcttac catgccggag cagaaaaata
    21061 cgcaactggc acaaatggca gacagtgagc gcgcgcagct gcttgccgag cgctttgatg
    21121 gcgtttgtgt gcatcaggaa agtgaaatcg ttcacgtatg gcgcggcggg gtatggtgtc
    21181 cggtcagcac aatggaactt agccgcgaaa tggtggtgat ctattcagag cacagggcca
    21241 ctttcagcaa gcgcgtaatc aataacgccg tggaagcgtt aaaagttatt gccgaaccaa
    21301 tgggcgagcc gtccggcgat ttgctgccgt tcgccaatgg tgcgcttgac ctgaaaacgg
    21361 gggaattttc cccgcacacg ccggagaact ggatcaccac gcacaacggc attgagtaca
    21421 cgccaccagc accaggggag aacatccgcg ataacgcgct aaactttcat aaatggcttg
    21481 agcacgcagc cggaaaagac cagcgcaaga tgatgcgtat atgtgccgcg ctgtacatga
    21541 ttatggcgaa ccggtacgac tggcagatgt ttattgaggc caccggagac ggcgggagcg
    21601 gtaaaagtac attcacacac atagccagcc ttctggcagg gaaacagaac acggtaagcg
    21661 ctgaaatgac atcgcttgat gatgctggtg gacgtgcgca ggttgtcggg agtcgtctta
    21721 tcgtcctggc tgaccagccg aaatatacag gcgaaggaac gggcatcaag aaaatcacgg
    21781 gcggtgaccc cgtggaaatt aacccgaaat acgaaaagcg tttcacggct gtaatcaggg
    21841 cggtggtgct ggcgaccaac aataacccga tgatattcac cgaacgggcc ggaggtgtgg
    21901 cacgtcgtcg cgtgattttc cgtttcgaca atatcgttag tgaggccgaa aaagacaggg
    21961 agctaccgga aaagattgcg gctgaaatcc ctgtcattat ccgccgattg ctggcgaact
    22021 ttaccgactc tgagaaagca cgggctttac tactggaaca gcgtgacggt gatgaagctc
    22081 tggcaataaa gcaacagacg gatccggtta ttgagttttg ccagttcctg aattttctgg
    22141 aggaagcacg cggcctgatg atgggcggcg gtggcgattc agtgaagtac acgaccagaa
    22201 acagccttta ccgcgtctat ctggcgttta tggcgtacgc aggcaggagc aaaccgctaa
    22261 acgtaaatga ctttggcaag gctatgaagc cagccgcgaa agtttacgga catgaatata
    22321 ttacgcggaa agttaaggga gtaacgcaga ctaacgcaat aacaacagac gagtgcgacg
    22381 cgtttttata attttttgta attgctgtct acctgagtaa agaaaaatac atttaattca
    22441 gtgcattaag ttaggtagat agcctttttt tactgtctac ccactatcta ccctctctac
    22501 ctgattttac ctgaatcaga cagggaggta gacacggggt agatagtgga taaaagcact
    22561 ctaccccgct gaaagcagcg ccattactgg catgagggcc actaaggtag ataaggtaga
    22621 caaggggagg cacaactcaa aactttttaa acgagggggt aaaaataaat atgcacactt
    22681 cagggaaatt taataaatca ttcaaaaaac gcagagacag aacagaaccg aaatatcgcg
    22741 cgttagacat gacagagcac gctttaaagg tggcaatcag aacgatagac cgccacgcgg
    22801 gggaaggata cgcgaaagca catcccgaac tgataagcgc attcatgacc acgacggcgg
    22861 caaattttgc cacgctgaca gagcgggaga ttgccgaagc ggaacaggta acaacaatca
    22921 acgttaaaac cggagaggtg gaatcatgag cgaaccaaaa tttggtgaaa aattttacaa
    22981 gcacaacgga cggatcacga ttctgcaaat aagcgcagct acaccaggct ggtgggttga
    23041 aacagacgaa ggatcctcac ccgtggcatc atgggagctt tgtgtgctta gttaccctga
    23101 ccgcgatgtg tatcaggata ttctgcctgt gatatccact gacaaaggca tgaaacccgt
    23161 agacatcaaa aacatggggt ttcagtgcgt aatgctcact gaaaaaatga tggaagagat
    23221 gaaaaagaac aatgccggat cgcttcactg aggtggcaat atgacagcac aaattgcagc
    23281 ttacgggcag ctggtgggcg acccgctggt aaaacagagc agcaagggca caccggagcc
    23341 gccaacggag aactacgcag acctgagaaa tgcagtaaat cgcacttacg cagacatggg
    23401 gctgagcaaa ttagcagagt aggaatatca gaaccacacc acgcagccgg agcaatccgg
    23461 ctttttttgc gtcatttgaa acggtatgga gcactactgg cttttcgtca cggtccggca
    23521 tagttactat ctgaaacaaa cagacacaac agaggaaaac gcaatgccga tgaaatttga
    23581 tgagatactt aaacagcgtg ataaatacca tgctgacaac atggagacga tgaatatcac
    23641 tgattaccgc gcatttctgg aaacgggggc gctgattgaa aaggatcatc atggttttgt
    23701 tcgttgcgcg ctatccggtg aaatgctggc agtaaatcct gaacagacag atgcattgat
    23761 agaatttctg aaagggatca gagactgagc acccatacag cacatacagc cggagcaatc
    23821 cggctttttg ttattttttg taaattattt gttcgtggtt gttccacgtt gttcactgag
    23881 cggatcggca tattttaccc gaactgaatc atgattattc tcgcccgtgg tgccaggacg
    23941 ctggggccac tttcccgcct gttaatgtgc tcgccaatat tcattaccag gcgggaaaac
    24001 gatcggtgcg attactgatt tccttatgaa aaacggttga gtgtttgccg cgtcctggag
    24061 ttccttactt aaccccagga ctttttttat gcagagaata atcgaattac gccagcagaa
    24121 aaccgccatt aaaaatcaga tgcgcgacat gctggagaac gctgaaaaag aaaaccgcag
    24181 tcttaacgat gctgagggcg caaaatttga cgaattacgc gctaaagctg aatccctcga
    24241 taaagacatt tcccgccttg aagccattgc agacgaagag cgcagcaagc caggtaaaag
    24301 cagccagacc actgaccccg cagaactacg taactacatt ctgaccggtg aaacccgcgc
    24361 attaagtaca ggcgttcccg ctgatggtgg ttataccgtt atccctgaac tgaacaccga
    24421 aatcatgcga atgctggcgg atgaatccac catgcgtcgc atctgtaccg tgaagaaaat
    24481 cagcagcaac gagtttaagc agcttgtttc cgctggcggt gcgaccgtta accacggtga
    24541 agagggtaag gcacgcgaac agaccagcac cccgcagatt aacgaggtga gcattaagct
    24601 gtatccggtc tatgcgtacc cgcgcaccac acaggaaatc gtggattttt ccgatgtgga
    24661 catcctttca tggctgacgg gtgagattgg cgacaccttc acggaaaccg aagaaagcga
    24721 tctggttgtg ggcgacggtg acaaaaaagc aaaaggtttt ttatccgtac cccgtgcaga
    24781 gaagaacgac aaagagcgtg attttggtac gttgcaggta attaaacctt ccgaatctct
    24841 ggcgtggaca tctgcggacc cgctgatcga cctgaaattt gcattacgta aaaaataccg
    24901 caaaaacgcg gtctgagtgg ttaactccac gacggcggca aaacttcaga aggtgaagaa
    24961 cgcgaacggt gattatatct ggcgcgaccg tttacaggcg ggtgatcctg atacgttgct
    25021 gggccttccg gtcgaatatc tggagtttat gcctgataac gttattgccc tgggtgactt
    25081 caaacgcggt tactacattg ttgatcacga aacaggtgtt cgcaccagac cggacaacct
    25141 gacagaacca ggattcatca aaattttcac gcaaaaatat ttaggcggtg gcgtggtgga
    25201 ttcgaacgcg atcaagattc tggcactgcc ggaagacgac gattaacagc atacagaagg
    25261 ggctaaaaag cccctttagt gttttatggg tgaaaaaatt atgaagagta tggaaatccg
    25321 gtcatcggaa atcaccacca gcggagccgg tacgctgacg ggctacgttg ttcgctggga
    25381 taagctttca gaactgttat ggggtgagtt ttacgaaaaa ttccagcggg gggcgtttac
    25441 tgaatggctt gcggcgggta atgacgttcg cggcctgtat gagcatgacc acagcatgtt
    25501 actggggcgc acccgttccg gcacgctgaa actggaagag gacgaaacag ggttacgctt
    25561 tgaactgacc ccaccggata ccagtacagg gcgcgacgtt atcgaactgg ttaagcgtgg
    25621 ggacatctcg gggatgagct ttggctttcg ctcccgtaag gatgtatggg ataccacaac
    25681 agatccatgc gtgcgcaccg tgctggtggc ggaactgtac gaaattaccg ttacatcggt
    25741 accggcttac cccgattccg gcgtggagct ggcccgccgt tccctgtatg agcagcaccc
    25801 cgaaaaaatg ccgcgtgcgg ataatcgccg ctggtgggcg gatttagcgg gggtgtgata
    25861 tgtggccttt cagacgaaaa aaagagcagc gcagcatgac gcttgatgaa tttatggcgc
    25921 tggccggcac atcgaacacg ggggcgggtg agtacgtatc atctgggaca gcggaatcac
    25981 tccccgccgt catgaacgcc gtcacggtca tctctgacgc ggtggctacc atgccgtgtt
    26041 acctgtacct ggtacgcaat gagaagggga aggaggcccg cgaatggctt gattctcatc
    26101 cggtcgatca catcctcaac gagcgcccga acgcgtggca gacaccctac cagtttaagc
    26161 gaatgatgat ccgctactgc ctgttaagtg gtaatgctta tgcggttatt cagtgggggc
    26221 gtgatggttt tccggcggct ttacatcctt acccgccgca gtcggtgaac gtggagcaga
    26281 caggtgatca taactggcgc tactgcatca ctgacgccta caccggaaac atccgcaact
    26341 atttaccgtg ggaggttctc caccttcgtt actccacgga tgacggtttt atggggcgct
    26401 cacctgtaac catctgccgc gaatcgctgg ggcttgggct ggcccaacaa cgccacggcg
    26461 cgagcgtgat gcgtgatggc atgatggcgg caggggttat cacgtcaggc gaatggctgg
    26521 acggcgtgaa gggcaaacag gcattagccg cactggaacg ctacaaaggg gccagaaacg
    26581 ccggaaaaac gcccatcctt gaagggggga tgagctatca gcagctgggc atgagtaatc
    26641 aggatgccga atggctggcc tcccgtcgct tcaccattga agacatcgcc cgaatgttca
    26701 acgtctcgcc gatttttttg caggaataca gcaacagcac ctacagcaac ttcagcgagg
    26761 caagccgcgc atttctcacc atgacgatgc gcccgtggct ggcgaacttt gagcagcaga
    26821 taaaaaacgc cttgctggtg gcctcgcctg tacctggtat ccggtatcag gtggagtttg
    26881 acagcgcgga cctgttacgg gccacacctg gcgaacgctt tgccacctat gagcgcggca
    26941 taaaatccga cgttatgtgt ccgaacgaag cccgcgaacg tgaagggctg tcaccgcgtg
    27001 atggtggtga tgagttcagc caggcatgga aacaggaagt aaaaatcagc gagggagaaa
    27061 aaccggaatg aacatagggc gactgcgtga caggataacg attcagaccc tgaaacagac
    27121 cagggatata accggcgaaa tactcgaaac gtgggaggac ggtcacacac tctgggcaag
    27181 cgtgaacatg gtcagcagca aggaggccat ttcatcgggc gcagagctgg cgattggtac
    27241 cgtaaggatc tggatacggt accggaagga catcaacgcc accagccgga taaaggtcag
    27301 tacggggccg ctggcggggc gtgtactgaa tatcatcggg cagccgctgc cggatgccgc
    27361 caggacacgc cttgaaattc tttgtcgtga gggcgcggaa aaatgacaga agaacttatc
    27421 accctggaag aagtgaaact ccattgccgc atcgatggcg acgatgaaga ccagttaatc
    27481 agcggataca ttgccgcatc gcttgaggcg tgccagatac acataggcag gcgctttgat
    27541 gacgggctgg cgttcacgcc agccataaag attggctgca tgatgtttat cgctcacctg
    27601 tatgaaaatc gccagattgt cgcggataac gcaaaaacac gcgtacccat gacgataggc
    27661 gcactctgga cggcttaccg tgatgtgggg gggtactgat gccgtggcaa ccattaagac
    27721 gatgcacaga accaggctgt aacaggcgcg ttaaatccgg caagtgtgag gagcacaggc
    27781 gggcggcatg gcgtgcagag gatgccagac gaggacaccg ccgcgcgcgt ggatattcca
    27841 gacagtggga caaataccgc gccatgtatc tgagtaaaaa cccgttatgc gtgcgatgcc
    27901 ttgagaaggg gatatatacg cccgccgtgg tggtggatca cattatcccg attaatggcg
    27961 gtgatgatgt tctcttctgg cccgaatgga accatcaacc attgtgccag gcgtgtcata
    28021 accagaaaac gaaatggctt gatccggcaa caaaaagcaa gcgtgccgca ggtggatttc
    28081 gtgaagagga agaacgggct gctaaccgca ataactggat gtatgacgct gatgaatgag
    28141 cgggaacaaa accgccttat ccgtggactt ataaggcagc gtgacgcatg gaagacacag
    28201 gaaacagggc ataaagataa agcgtcagga cgcgcagaac gcatcacagc gaagcgatta
    28261 accgaccgtg accgcgaggt tatggaatgt ttccgcaatc gctgatgagg ctgtatgacg
    28321 gggtgggggg cgttttcagg acaaacccga ccccgcctac ctggatgata tcgccgtgaa
    28381 gcagtggcgg gaaaaatcgc ggcagcttgc ggaacgggga gacctgaccc ccgccgactg
    28441 gagcaatctg gaactgtatt gcgtcaacta ctccatttac cggaaagccg ttgcagacct
    28501 tgcggcgcgc gggttcagca ttgttaacag tcagggcggc gagagcagaa accccgcatt
    28561 aagcgcaaaa tccgacgctg aaagagtgat gataaaaatg gcctccttgc tcggttttga
    28621 cccgataagc cgccgtaaaa atccaccgga aacagaagaa gaggacgagc ttgaccgcct
    28681 ggaataagta cgcagaagac gtaaaaacgg gcaaaattcc ggcctgtaaa cggttaaaac
    28741 aggctgttaa acggtacttt tcggacctta aaagccccct ttacacgttc gatcgtgagg
    28801 ttgtggagcg gtttattgcc ttttccaggg tgtgcccgca cgtaaaagga ccgatgcgtg
    28861 gcagacccat tgagctggag ccgtggcagc agttcgcctt tgcgtgcatc ctcggcttta
    28921 aggttaaggc caccggacgg cgcaaataca cgagcgcctt tatcgaagtg ccgcgcaaaa
    28981 atgcgaaatc cacgaccgcc gcgattctgg ctaactggtt tctgattatg gagaacggtc
    29041 agcaggatat ctacaccgca gcggtgagcc gtgaccaggc gcggatcgtg tttgataatg
    29101 cgcgtcagat gtgcctttta tcccgaccgt tacgcaggcg ggtgaatatt caggcgcata
    29161 aggtgataca cccgaaaagc aacagcctgt taaagccgct ggcagcaaaa gcggcaacca
    29221 tcgaggggac aaaccccagt ctttccatcg tggacgaata ccacctacac cccgataacg
    29281 gcgtttattc cgcgcttgag ctggggatgg gcgcacgtcc cgaagggctg ttatttgcca
    29341 ttaccacatc gggcagtaac gtcgtttcag cctgtaaaca gcactatgat tattgctgcc
    29401 agatcctgga cggcgaagag gttaacgatt caatttttgt actgatttac gaactggacg
    29461 acgaaaacga ggttgatgat ccggcgatgt ggataaaggc gaaccctaat attgatgttt
    29521 ccgtggatcg tgaaaaactg gcctcaacca tccagaaagc gcggggtatt ccgtcgcagt
    29581 gggtggagat gatgacaaag cgatttaata tctggtgtca ggggactacc ccgtggatgg
    29641 gtaatggtgc atgggctgag tgtgccggaa cgttcacgga ggaagattta cacggtcagg
    29701 agtgttacgc ggggctggac ttatcatcaa ccagcgatat ttccagcgta tgctatgcct
    29761 ttccggtcgg taaaaccatt atgctgattt cccgtcatta tctgccggag ttccagctac
    29821 agaaccccgc caataaaaac cgcgctgtct atcgccagtg ggctaaagcg ggctggatac
    29881 gcacaacgcc aggtgactgc attgattacg accggatcag agatgacatc atgcaggatg
    29941 cggagaaatt taatatcagg ctggtgggct ttgatacgtg gaacgccacg catctcagga
    30001 cgcaattaca gggggctggt tttgaggtgg agccgttccc gcaaacctac ctcagattca
    30061 gtccggcggc gaaatcgttc gaagtttttg ttaaccgcag ggtgattgtg catcgtggcg
    30121 atccggtgtt gtcctggtcg atgagtaacg tcgtgatgca gagtgacgcg aacgccaata
    30181 tcaagccgaa caagaaaaaa tcaccgaaca agatagaccc gagcgtagcg gcgctgatgg
    30241 cgtttggtac attccaggca gagcatgagg attttgcttt tgatataagc gacagccacc
    30301 gccagaaact ggaagaattt agcggggtgt aatgaggtca gcagcatgac agaagccgaa
    30361 atactgcgat taatccgccg cgtctctgga atcagccagc aggctgacga acagaccacg
    30421 cagccggaca gcgtgacagc cgaaaattat gcgcgtgttg ttgctgaggt gatgcgccgt
    30481 gatggtatcc agcttaatga tgcggatatg cgcgacatac ggatccgcgt tctggaaatg
    30541 ctggcctaca atcgccgcgt tgaactgtat cgggagaagg agaaaatcac gtaccactgg
    30601 aagaagccgg agcggttacg gcggtaactc actgagatta aagcaaagcg caaaattgcg
    30661 ctggctacat ccctcacatc tgaggactgt taaccagcag gccggaatat attcagaaac
    30721 tacgaaattt tcgcagttta attactcgtc agcttaatcg ctaacccgct ctgatatttt
    30781 cggaaacctc aatttgagga agtcggcgag gtaactgttt gatattcccg atagcgcaaa
    30841 gatgcgcggt cgggcagaat gaccaccacc tcaatcgtga cggagagtga caaccagtga
    30901 cacaccgtga caaaaagacg gtttaaccgt tcattaaaaa tcgacgtgat tttgtgcttt
    30961 ccggatgacg tggccaacgt cattttttag cgaaaaattc tgattaaggt cactggctga
    31021 acggtcataa tgaccacgca gaccatcgag gcaaccatag cgatcatcgt tacggttgga
    31081 gcttttccgc cattggtgga aaaggtgcac atctggatca tcattacggt gatcaagggt
    31141 atgtaaaacc caccagcctg attaaaggtt aaccggaaaa aaaatccggt agccccgtta
    31201 tggggatccc catatcgaca ttaacgcccc tcatgaattg gtgcgcttcc ccctggaaag
    31261 attacccgcc tgtatatttc ttgtgtctat ttgttccacg ttgtttcata cagtgcaccg
    31321 aacggtgtag ttaatggtgt agtcaatttg cgatttttgg cactttttca atgaaaattg
    31381 tttgatcgaa tcaatgactt gatgtagtgg taaaaggaca tatttattca tctttcggcg
    31441 cagaatgctg gcgaccaaaa atcacctcca tccgcgcacc gcccagcatg ctctctccgg
    31501 cgacgatttt accctcatat tgctcggtga tttcgcgggc taccgccagc cctacacctt
    31561 gcccagggcg taaagtatca acccgttgac cacggtcgaa aatgacctct cgcttgctta
    31621 atggaatacc ggggccatca tcctcgacca caatatagag atgctcgtcg gtttgccttg
    31681 cagaaatttc gacaaactcg aggcaatatt tacaggcatt atccagcacg ttgcccatca
    31741 cctcgacaaa atcgttctgc tcaccgacaa agctgatctc tggcgaaata tcgagagaga
    31801 tattgacccc tttgcgttga tacactttgt tcagcgctga ggtgagattg tccagcagtg
    31861 gggcgaccgg atgcagctcg cggctgagca atgtcccgcc gcgcatactg gcacgatgca
    31921 ggtagtagcc aatttgctgt gaaatgcggc tgatttgctc cagcattacc ggctcagcat
    31981 cactgacgct catcttttca ctacgcagag aacgcagcgt actttgcagc accgccagtg
    32041 gcgttttcag actatgggtc aggtcggtga gcgtcgtacg gtatttgtcg taacgttcgc
    32101 gttcactttt taacaatcgg ttcaggtttc gtaccagact ggtcagttct cgcgttgtgg
    32161 ctggattgag caattcgcgg ttatgttctt ccagttcgcg gacttctttt gccagggctt
    32221 cgatggggcg taaactccac caggcggcga cccacagcag cgggatcact aacagcagat
    32281 tggctgagag cacatagata aaccagctcc agaccatata ggaacttttt agctccaccg
    32341 gaatggtatc caccaccaca atggttaatt ttggcatccg cgatgttgcc gggtagacgt
    32401 ttaccgccac cgagtgggtc atctccgcgt cgtcatcatc ttcccgcact tcctgcaact
    32461 gttgctgtat cgaatgatct ccactcagca agaggctggt atcgttaaca tccgcttcaa
    32521 tttcatgaaa accattcgat ttcagccagt caggctggat cattttcatc agccagggca
    32581 cgtcacgttg cgcccataaa agctgcccgt tctcatcata aattagcgtc atggtggggc
    32641 tttgcttgtc gatattttcg ggtaactcga catgcaactt attgttttcc cactgcgcaa
    32701 gggtatagaa cagattgctc tcgccacgta acagccgaaa cgtagtttta tcgaaactga
    32761 cgctataacc gatcagcgcg accattccgt aggcaagcga aagcaccagt actaccgctg
    32821 ccgttgccaa cagaaaacgt acccgcagcg agagcgggaa aaaaagacgc agtaattttt
    32881 tcatcagcgc aattcgaaca ggtagccctg gccgcgaacg gtggtaatga cttcttgcgg
    32941 atattgtgcc tgaatttttt tgcgcagacg tcccatcagt acatcaatgg tatggctttc
    33001 ccgcagctcc gcatccggat agagttgtag cattaacgaa tctttgctga ccactttgcc
    33061 attattgcgt atcaacgttt ccataatggt gtattcgaac gcggtcagtt tgatcacttc
    33121 gtcattaata gataattcac ggcgagagag atcaacctga aacgggggga gcgaaatgac
    33181 ctgtgaagcc agaccgctat tacgccgcat taatgcctgc attcgcgcca tcacctcttc
    33241 aatatgaaac ggtttagtca cgtaatcatc agcaccggca cttaatactt cgactttgtc
    33301 ctgccagctt tcacgggcgg ttaataccag aatcggcagt gaaacatcgt tgctacgcca
    33361 gcggcgaatc agtgacagac cgtcctcgtc aggcaatccg agatcgacaa tcgcaatatc
    33421 cggtaaatgt tcattgagat aataatcggc ttctttggca tcttctgcgt catcgacctg
    33481 atgaccagca tcctgaatct gaactttaag gtggtgacgt aacaacgcat tgtcttcaac
    33541 aaccagtacg cgcattttta tttctccctg tcttaattat taaaatagtg taacgcgatt
    33601 atgtgtttat gggggtaaac attaaataaa ccagcgggga ggggaggtaa agtgaaaaaa
    33661 gaaaaagtgg ataatcttaa taagcaggcc ggacagcatc gccatccggc actgatttga
    33721 ggcttatttc agctcatcaa ccatcgtgat agctcgacca atatagttag ccggcgtcat
    33781 cgctttcagg cgggctttct cttcttctgg caacgccaga ccatcgataa actgcttcat
    33841 gccttcggcg tcaacgcgct taccgcgagt aagctctttc agcttctcgt acggtttttc
    33901 gatgccatag cgacgcataa ctgtctggat tggttctgcc agcacttccc agttgtgatc
    33961 cagctcatcc agcagatggt cacggttcac ttccagtttg ctcacgcctt tcagggtgga
    34021 ttgatacgca atcagcgcat aaccgatacc cacgccgagg ttacgcagca cggtggagtc
    34081 ggtcaggtca cgctgccagc gggaaaccgg cagtttgctt gccagatgct gcaataccgc
    34141 gttggaaagg cccagattcc cttcggagtt ttcgaagtcg atcgggttaa ctttatgcgg
    34201 catggtggaa gaaccaatct caccagcaat ggttttctgt ttgaagtggt taagggcgat
    34261 ataaccccag acgtcacggt caaagtcgat cagaatggtg ttgaagcgcg caacgcaatc
    34321 aaacagttcg gcaatgtagt cgtgcggttc aatctgggta gtgtacgggt tccattgaat
    34381 acccagcgag gtgacgaact cttcgctgaa ctgatgccag tcaacttccg ggtaagcggc
    34441 gatgtgggcg ttatagttac cgaccgcgcc gttgattttg ccgaggatct ccacctggtt
    34501 aagctggcgg tgctggcgct ccatacggta ggcgacgttt gccatctctt taccgatggt
    34561 tgacggcgtg gctggctgac cgtgggtacg agacagcagc gggatatcgc ggtactgaac
    34621 ggcgagatct ttaatgccat caatcagttg acgccagtac ggcaggatca cttcatcacg
    34681 cgcggttttc agcattaatg cgtgggagag gttattgata tcttccgaag tacaggcaaa
    34741 gtggatgaat tcagaaaccg cgtgcagttc cgggatctcc gccacttttt ctttcaggaa
    34801 atactcaacc gctttaacgt cgtggttagt ggtacgctcg atggttttga tgcgtgcggc
    34861 atcttcttcg ctgaaattgg cgacaattgc atcaaggtaa ccgattgcgt cggcagcaaa
    34921 agcaggaact tccttgatcg ctgcgtgcgc ggccagtttt tgcagccaac gtacttcaac
    34981 ttgtacacgg aatttcagca aaccatattc gctgaaaatc ccgcgcagcg cgctgacttt
    35041 atcgccgtag cgtccatcga caggggaaac ggcggtcagt gaggataatt ccatagatca
    35101 caactccggg gttaaatgag caagaatttg ttttgcctga gtggtcaggc gattacgaga
    35161 aaacatcagt tgtagacgtc cgccgccgac ctggtgccag agcacggcgg cgcgaatgcc
    35221 tgccagcagg gttgcgcgaa ctttcgcctg cacttgtggg ctttgcagta cagcagggga
    35281 accggtgacc tgaatgcgcg ggccaagcgg gctgatcaca tcaacataga tagcagccat
    35341 cgcgctcatc agcgtttcgg actgtaaatc gaagtgttcg agctggcgtt gcaggccgtt
    35401 gatccggttg cccagagtgt cgagcgcgcc tttcgctgag gagagtttgc gctcaagcac
    35461 catcaagctg agtgtgtagc gggttaattc ggcgtttaag ccctggcaac tgctggcatt
    35521 gagcacgccg agcagggttt ccagcccgac gcgcaggttg gcttcgctac cgccaaaaac
    35581 cgccagcgtc gagctggggt tcatatcaat aatactgttg agtgagacgt gtagcgcatc
    35641 ggcatcacaa tgcccctggt gagcgagttg ttgcaccagg cgtgccgact gacaaatacc
    35701 ggccagggcg agggtgatgt catagtaatt ctttgccacg ttcactgctt ccttgtttta
    35761 agtaaagata ataatcagac cggcagcggc agacgctgct caataatacc gccaccgagg
    35821 cacacttcac cgttatagaa gacggcagac tggcccggcg tcacggcggc aaccggctca
    35881 tcgaaaatca ctttaatgcg atcgtcgtcc agcgccttga cggtgcacgg aatgtcggtc
    35941 tggcgatagc gggtttttac cgtgcaacgc atagtgccgg tgaatggttc gcgatcgacc
    36001 cagtgcagct gctgggcaat caacccgaca gacatcagcc gcgggtgttc atggccctga
    36061 gcgacaacca gaatgttgtt ttcgacgtct ttgtccacca cataccacgg ttcttcggta
    36121 ccttctttgg tgccaccgat acccagacct ttacgctgac cgagagtgtg atacatcagc
    36181 ccctggtgct caccaatttc atcgccatcg acggtaatga ttttgcccgg ttgcgccggg
    36241 agataacggc ccaggaactc gcggaattta cgttcgccaa tgaagcaaat gccggtagag
    36301 tcttttttct tcgcggtgac cagaccaaga tcttcagcaa tcttacgcac ctgcggtttt
    36361 tccagttcgc ctaccgggaa caggctttgc gcaatctgct catggctgag cgtataaagg
    36421 aagtagctct ggtctttatt gctgtccaga ccacgcagca ggcggctctt gccatcgaca
    36481 tcggcccgac gtacgtaatg accggtagcg atataatcgg cacctaaatc ttcggcggca
    36541 aattcgagga aggctttaaa tttgatctcc ttgttgcaca gaatatccgg attcggcgtg
    36601 cgaccggctt tatattcggc aaggaacagt tcgaagacgt tgtcccagta ctcggcagca
    36661 aagttaacgg tgtgcagttc aatgccgagc ttgtcgcaga ccgcctgggc atcagccaga
    36721 tccgctgctg ctgtgcaata ttcctcaccg tcgtcttctt cccagttctt cataaacagg
    36781 ccttcgacct gatatccctg ttgttgcaac agccaggcag aaacggagga atcgacaccg
    36841 ccggacatgc cgacgattac tttttttgcg gtttcagaca ttggatcact cacgacattg
    36901 aacttcaagg cggcgtattc tatcacgcag ccttttagat gacacccttt gtaaaaggcc
    36961 agttaaaatc gccaatcatc tccagcggat aacgttgccc gctttgataa caacgaatac
    37021 tttccgccac cagcggcgaa cgaagatttg acgcctgtaa aatttcttcg gcgctgaccc
    37081 aacggcagca gtcgatatcg ctgtcatgag gctgggtcgg gcatatttgc tcaagctcaa
    37141 tggcaaagag gaaacgcaaa aacggcgttt tatctggcgc aatccactga tgcatacgaa
    37201 taaagtgttg cggctgcgcg ctgatgccgg tttcttccca cagttcacgg gcggcggctt
    37261 ccactaaggt ttcatcggct tccagatgcc cggcaggttg gttccataac gctttaccat
    37321 taatcgtctc ttcaacgact aaaaatttgc cttctgcgtg caccacgcaa gcaacggtaa
    37381 cgtgcggttt aaacattttg catccttaat ctgtcacttc tcgccattcg ccattggcaa
    37441 gattatccaa agagtaatct cccatcgcat agcgaatcag tcgcagcgtg gggaaaccaa
    37501 catgggcggt catgcggcgc acctggcgat tacgtccttc atataaggtg atcttcagcc
    37561 agctggtggg aatacttttg cgttcacgaa ttggtggatt ccgcggccat aaccacgcgg
    37621 gttcgtcaac cagctccgcg cctgcgggca gggtagggcc atcatttaag gttacgccat
    37681 tgcgcaaggc ttcaagtgcg tcttgtgtgg gaataccttc cacctgcaca taatagattt
    37741 ttccggtgcg tttacccggc tgggttaaac gcgcctgcaa cgcgccgtta ttggtcagca
    37801 ccagcaaccc ttcgctatcg cggtcaaggc gacctgctgc ataaacgccc tgaaccggga
    37861 tgaattcttt taatgttttg cgtccggttt catcggtgaa ctgcggaaga acatcgtagg
    37921 gtttattgaa gaggatcaca cgcgtgggct ggttttcatg tttacgcctg gtagaacgtt
    37981 gcgagctgaa tcgcttaacc tggtgatttc taaaagaagt tttttgcatg gtattttcag
    38041 agattatgaa ttgccgcatt atagcctaat aacgcgcatc tttcatgacg gcaaacaata
    38101 gggtagtatt gacaagtcaa ttacaaatca ttaacaaaaa attgctctaa agcatccgta
    38161 tcgcaggacg caaacgcata tgcaacgtgg tggcagacga gcaaaccagt agcgctcgaa
    38221 ggagaggtga atggaaagta aagtagttgt tccggcacaa ggcaagaaga tcaccctgca
    38281 aaacggcaaa ctcaacgttc ctgaaaatcc gattatccct tacattgaag gtgatggaat
    38341 cggtgtagat gtaaccccag ccatgctgaa agtggtcgac gctgcagtcg agaaagccta
    38401 taaaggcgag cgtaaaatct cctggatgga aatttacacc ggtgaaaaat ccacacaggt
    38461 ttatggtcag gatgtctggc tgcctgctga aacccttgat ctgattcgtg aatatcgcgt
    38521 tgccattaaa ggcccgctga ccactccggt tggtggcggt attcgttctc tcaacgttgc
    38581 cctgcgtcag gaactggatc tctacatctg cctgcgtccg gtacgttact atcagggcac
    38641 tccaagcccg gttaaacacc ctgaactgac cgatatggtt atcttccgtg aaaactcgga
    38701 agacatttat gcgggtatcg aatggaaagc tgactctgcc gacgccgaga aagtgattaa
    38761 attcctgcgt gaagagatgg gcgtgaagaa aattcgcttc ccggaacatt gcggtatcgg
    38821 tattaagccg tgttctgaag aaggcaccaa acgtctggtt cgtgcagcga tcgaatacgc
    38881 aattgctaac gatcgtgact ctgtgaccct ggtgcacaaa ggcaacatca tgaagttcac
    38941 cgaaggcgcg tttaaagact ggggctacca gctggcgcgt gaagaatttg gcggtgaact
    39001 gatcgacggc ggcccgtggc tgaaagttaa aaacccgaat accggcaaag agatcgtcat
    39061 taaagacgtg attgctgatg cattcctgca acagatcctg ctgcgtccgg ctgaatatga
    39121 tgttatcgcc tgtatgaacc tgaacggtga ctacatttct gacgctctgg cagcgcaggt
    39181 tggcggtatc ggtatcgccc ctggagcaaa catcggtgac gaatgcgccc tgtttgaagc
    39241 cacccacggt actgcgccga aatacgccgg tcaggacaaa gtaaaccctg gctctattat
    39301 tctctccgct gagatgatgt tacgccatat gggctggact gaagccgcag acctgattgt
    39361 taaaggtatg gaaggcgcga tcaacgccaa gaccgtaacc tatgacttcg agcgtctgat
    39421 ggaaggcgct aaactgctga aatgttcaga gtttggtgat gcgatcatca agaatatgta
    39481 atcactacat gtgttaaata tttaaacggg cgtataacac gcccgttgtt ttatttatgt
    39541 ggatattatt aatagcatat cgagcata
//
LOCUS       DAZPEU010000020        37776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPEU010000020 DAZPEU010000000
VERSION     DAZPEU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.020"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..73)
                     /locus_tag="ACHU2L_000915"
     CDS             complement(<1..73)
                     /locus_tag="ACHU2L_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR1355884.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACHU2L_000916"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACHU2L_000916"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR1355885.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="ACHU2L_000917"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="ACHU2L_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR1355886.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACHU2L_000918"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACHU2L_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR1355887.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="ACHU2L_000919"
     CDS             2703..2939
                     /locus_tag="ACHU2L_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR1355888.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="ACHU2L_000920"
     CDS             2942..3799
                     /locus_tag="ACHU2L_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1355889.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="ACHU2L_000921"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="ACHU2L_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="ACHU2L_000922"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="ACHU2L_000922"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR1355890.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="ACHU2L_000923"
     CDS             complement(6414..7310)
                     /locus_tag="ACHU2L_000923"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR1355891.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="ACHU2L_000924"
     CDS             complement(7359..7598)
                     /locus_tag="ACHU2L_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR1355892.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="ACHU2L_000925"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="ACHU2L_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR1355893.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="ACHU2L_000926"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="ACHU2L_000926"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR1355894.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="ACHU2L_000927"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="ACHU2L_000927"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR1355895.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="ACHU2L_000928"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="ACHU2L_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR1355896.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..15223
                     /locus_tag="ACHU2L_000929"
                     /pseudo
     CDS             10765..15223
                     /locus_tag="ACHU2L_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15267..16475)
                     /locus_tag="ACHU2L_000930"
     CDS             complement(15267..16475)
                     /locus_tag="ACHU2L_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1355897.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16498..16908
                     /gene="tnpA"
                     /locus_tag="ACHU2L_000931"
                     /pseudo
     CDS             16498..16908
                     /gene="tnpA"
                     /locus_tag="ACHU2L_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(17022..17369)
                     /gene="grxD"
                     /locus_tag="ACHU2L_000932"
     CDS             complement(17022..17369)
                     /gene="grxD"
                     /locus_tag="ACHU2L_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR1355898.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17704..18531
                     /gene="mepH"
                     /locus_tag="ACHU2L_000933"
     CDS             17704..18531
                     /gene="mepH"
                     /locus_tag="ACHU2L_000933"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR1355899.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18659..19240
                     /gene="sodB"
                     /locus_tag="ACHU2L_000934"
     CDS             18659..19240
                     /gene="sodB"
                     /locus_tag="ACHU2L_000934"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR1355900.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19386..20555)
                     /locus_tag="ACHU2L_000935"
     CDS             complement(19386..20555)
                     /locus_tag="ACHU2L_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1355901.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20721..20810)
                     /gene="cydH"
                     /locus_tag="ACHU2L_000936"
                     /gene_synonym="ynhF"
     CDS             complement(20721..20810)
                     /gene="cydH"
                     /locus_tag="ACHU2L_000936"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR1355902.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            21109..22134
                     /gene="purR"
                     /locus_tag="ACHU2L_000937"
     CDS             21109..22134
                     /gene="purR"
                     /locus_tag="ACHU2L_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR1355903.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22131..23063)
                     /gene="punR"
                     /locus_tag="ACHU2L_000938"
     CDS             complement(22131..23063)
                     /gene="punR"
                     /locus_tag="ACHU2L_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR1355904.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23176..24387
                     /gene="punC"
                     /locus_tag="ACHU2L_000939"
     CDS             23176..24387
                     /gene="punC"
                     /locus_tag="ACHU2L_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR1355905.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24678..25826
                     /gene="cfa"
                     /locus_tag="ACHU2L_000940"
     CDS             24678..25826
                     /gene="cfa"
                     /locus_tag="ACHU2L_000940"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR1355906.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25866..26507)
                     /gene="ribE"
                     /locus_tag="ACHU2L_000941"
     CDS             complement(25866..26507)
                     /gene="ribE"
                     /locus_tag="ACHU2L_000941"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR1355907.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26722..28095
                     /gene="mdtK"
                     /locus_tag="ACHU2L_000942"
     CDS             26722..28095
                     /gene="mdtK"
                     /locus_tag="ACHU2L_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR1355908.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28136..29392)
                     /locus_tag="ACHU2L_000943"
     CDS             complement(28136..29392)
                     /locus_tag="ACHU2L_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1355909.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29700..29776
                     /locus_tag="ACHU2L_000944"
     tRNA            29700..29776
                     /locus_tag="ACHU2L_000944"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29734..29736,aa:Val,seq:gac)
     gene            29781..29857
                     /locus_tag="ACHU2L_000945"
     tRNA            29781..29857
                     /locus_tag="ACHU2L_000945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29815..29817,aa:Val,seq:gac)
     gene            29965..30270
                     /locus_tag="ACHU2L_000946"
     CDS             29965..30270
                     /locus_tag="ACHU2L_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR1355910.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30396..31818
                     /locus_tag="ACHU2L_000947"
                     /pseudo
     CDS             30396..31818
                     /locus_tag="ACHU2L_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31830..32642)
                     /gene="ydhT"
                     /locus_tag="ACHU2L_000948"
     CDS             complement(31830..32642)
                     /gene="ydhT"
                     /locus_tag="ACHU2L_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR1355911.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32646..33431)
                     /gene="phsC"
                     /locus_tag="ACHU2L_000949"
     CDS             complement(32646..33431)
                     /gene="phsC"
                     /locus_tag="ACHU2L_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR1355912.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33428..34096)
                     /locus_tag="ACHU2L_000950"
     CDS             complement(33428..34096)
                     /locus_tag="ACHU2L_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1355913.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34160..34798)
                     /locus_tag="ACHU2L_000951"
     CDS             complement(34160..34798)
                     /locus_tag="ACHU2L_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR1355914.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34811..36912)
                     /locus_tag="ACHU2L_000952"
                     /pseudo
     CDS             complement(34811..36912)
                     /locus_tag="ACHU2L_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36933..37559)
                     /locus_tag="ACHU2L_000953"
     CDS             complement(36933..37559)
                     /locus_tag="ACHU2L_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR1355915.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9062 a         9889 c         9293 g         9532 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cactccagag ctgcttgtac
    15541 tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt taaacctgat
    15601 tttgcccgga cattgcgccc cggctggctg accgtacccg ctgctgactt tgacatgttg
    15661 ctgaccttca aatcctcaat gactatcatt gcgtggtttt tgctgacggt cgttgtgact
    15721 ttgtgaaggt agtccctgcg gatatttgcg atacaggaat gcagtcgctg tattttgcgt
    15781 ttctgcttct gccagttgtt gctgaatttg accttccggc ttaactgtcg ctgaagtctc
    15841 gccagcgtct tctggttttt ttgaaaactg tttacaggct caaagactgt gccatctgac
    15901 agcgtggcga gtttagccac gccagcatcc agcccgatca ttgatgctga agggtgaacc
    15961 ggagttgata cttcactttc tgtctgaata ctgatgtacc acgtaccgca ggactggctg
    16021 acagtgacat ttttcacaac tcccgtgacc tgacggctat tccggtagcg catccagcca
    16081 agtttcggca gaaaaatacg gctgttttcc tgatcgagct taacgtcctg cgggtagcgg
    16141 aatgcagcat tctgtccccg ctttttgaat cggggaaaag cagcccgctt ctggaagaag
    16201 tttttgtaag cccgctcaag gtctttcagt gactgttgca atggctgtga gggcgaatct
    16261 ttaagccatt gcgtttcagt ggcgtttttc cactcaacca gccaggaagc cattttcccg
    16321 taagggatgt atttattacc ggcctcatga ttctcattct gaagcgccag agcacgattg
    16381 aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac gctcctgttg accaccgggt
    16441 cttaactgga atttaaatgc ctgtaatcgt ttcacaggtc aaatgatact ttggtctgtg
    16501 aaaaaagaaa ccgatatccg gcgtggaagg cattgtgttt tcctgatgca tgttcacctg
    16561 gtctttgtca ccaaataccg acgccagatt tttgaccatg acacgacaga aaaactacgc
    16621 acttactttt caaatgtatg tgctgatttt gcagctgaac tggttgaaat ggatggcgaa
    16681 ccagatcacg tccatttgtt aattaactat cctcccaaac tggcgatatc cagtctggta
    16741 aacagcctca aaggcatatc gaataggtta ctgcgacgag atcgaccaga tattgcagtc
    16801 aggtattact acaaaggcgt tttgtggagt cctggctatt ttgccagtag ctgcgaaggt
    16861 gcgccaatat ccgtcatccg ccagtacatt gaacaacagc aaacacctgg tcaggtggaa
    16921 aaccgcgcct tatatccccg ccctgaagga cgaggtttta cggcgcaccg gataaaggac
    16981 tgacggatgc ccgttcgcat ccgtcagtat tgcaggacgg attattccgc gtccggctct
    17041 tcagacttgt atttagcggc agtttctttg atcagctgct gcagttcgcc acgctgatac
    17101 atttcgatca cgatatcaca accgccgacc agctcgccgt caacccacag ttgcgggaag
    17161 gtcggccagt tagcatattt cggcagttcc gcacgaatgt ccggattctg cagaatatca
    17221 acataggcaa aacgttcgcc acatgcggca agcgcctgga ctgcctgggc agagaaaccg
    17281 cagctcggca gtttcggtga acctttcatg tacagcagga tcgggttttc agcaatctgg
    17341 cgttggattt tttcgatagt ggtgctcatt gtcttgcttc ctcaaacttc tttacggcag
    17401 taatctgaca ttgtagcggg tcagtgcggc atcggaaaat aacattttca tcacgctttt
    17461 gctattttat ccctttgctc tatttgttgc atttcaaata ttcgtttctt ctctgatgcg
    17521 cggcaaactg gcgtatttat aaccattttt agctttcact gctgcatttt tttgcgctcg
    17581 ccaacgaaac gtatttttta acaataaaag ctattaactt tctcttcttc tatgcattag
    17641 aatcatcaag ttttgtaaat cagacgcagg catgatagac ctgccttaac agagggacgc
    17701 tcagtggcgc ggataaaccg tatttcgatc acgctctgtg ctttactttt taccaccctg
    17761 cctttaacgc ctatggccca tgcttcaaag caagccaggg agagttctgc taccactcat
    17821 atcaccaaaa aagcagataa aaagaaaagc acggcaacca ccaaaaagac ccagaaagcc
    17881 agccagaaaa cagcgaaaaa agccgccagt aaaagtatga ccaaaagcaa aaccgcttct
    17941 tctgttaaaa aatcttccat taccgcttct aaaaacgcca aaactcgcag caaacacgcc
    18001 gtcaataaaa cggcctcagc cagctttacc gaaaagtgta ccaaacgtaa gggctataaa
    18061 tcgcattgtg tgaaagtcaa aaatgccgcg tcaggaaccc ttgccgacgc gcacaaagcg
    18121 aaggtgcaaa aagccacgaa agtggcaatg aataaactga tgcagcaaat tggtaagcca
    18181 tatcgttggg gcggcagctc accgcgtacc ggttttgatt gcagcggcct ggtttattac
    18241 gcttataaag atttggtgaa aattcgtatt ccgcgtacgg cgaatgaaat gtatcacctg
    18301 cgtgatgcag gacctatcga acgtagtgag ctgaaaaacg gcgacctggt ctttttccgt
    18361 actcagggac gcggtacagc cgatcatgtc ggcgtgtatg tcggcaacgg caaatttatt
    18421 cagtcaccgc gcacaggtca ggaaattcaa atcacttctc tcagtgaaga ctactggcag
    18481 cgccactatg ttggcgctcg tcgggtaatg accccaaaaa cacttcgcta aaactttacc
    18541 ctgttgttac ggcaacaggg taagttcatc ttttgtctca ccttttaatt tgctacccta
    18601 tccatacgca caataaggct attgtacgta tgcaaattaa taataaagga gagtagcaat
    18661 gtcattcgaa ttacctgcac taccatatgc taaagatgct ctggcaccgc acatttctgc
    18721 ggaaactatc gagtatcact acggcaagca tcatcagact tatgtcacta acctgaacaa
    18781 cctgattaaa ggtaccgcgt ttgaaggtaa atcactggaa gagattattc gcagctctga
    18841 aggtggcgta ttcaacaacg cagctcaggt ctggaaccat actttctact ggaactgcct
    18901 ggcaccgaac gccggtggcg aaccgactgg aaaagtcgct gaagctatcg ccgcatcttt
    18961 tggcagcttt gccgatttca aagcgcagtt tactgatgca gcgatcaaaa actttggttc
    19021 tggctggacc tggctggtga aaaacagcga tggcaaactg gctatcgttt caacctctaa
    19081 cgcgggtact ccgctgacca ccgatgcgac tccgctgctg accgttgatg tctgggaaca
    19141 cgcttattac atcgactatc gcaatgcacg tcctggctat ctggagcact tctgggcgct
    19201 ggtgaactgg gaattcgtag cgaaaaatct cgctgcataa taactgatgg caaatgcagc
    19261 attgcctgat gcgcttcgct taccatgcca acgcggatga ggcgttaaca gcgcattcgg
    19321 caagataaat cgcacgttgt cagcaactgt aacgcagaag gttatccttc tgcgtttttg
    19381 tttaattagc tgttagcaac gcaaactgtt tcaggttgtt ttctggctga cataaacacc
    19441 agcaataatg ccagtcccgc gacaatcgcc cccgtcaccg gcacaaagct gtatcccagc
    19501 ccagcggaaa ttaccgcacc accagcagct gctcccagcg catttccaag attaaaggca
    19561 ccaatattga ctgatgaaga cagacctggc gcttcactgg cgacatgcat cacgcgcatc
    19621 tgtaacggag gtacaaccgc aaaggttgct gcgccccaca ccaccatgct aatagctgcg
    19681 ccgaactcat tgcgggccag gaacgggatt gccagcataa tcaccatcag cagcaacaaa
    19741 aagcctttca acgtgccgtt aactgaacga tctgccagtt tgccgccgag atagttaccg
    19801 atagagaatc cgacaccaat cagcaccagc attgccgtga cgaacaccgg tgttgcgtgg
    19861 gtaatacttt gcagtaccgg agagatatag gtgtagaggg taaacattgc accagctccc
    19921 agtaccgtcg tcagcaatgc agacagcacc tgcggacgca ttaataccgc cagctctttt
    19981 ttcacttcag gtcgtgcccc tgtaccacct ttaggtaatg agaagaacag acttaccatt
    20041 gaaatcactc ccagccccgc cgttgccaga aatgacatcc gccagccgat ggtttcaccc
    20101 aaccaggtcg ccgccggcac gccaccgata tttgccaggg ttaaccccat aaacatagtg
    20161 gcaactgcgc tggcctgttt atgttttggc accacgcttg cggccacgac tgaacccaaa
    20221 ccaaaaaatg ctccgtgatt caggctggtc aaaatacgtg aaagcatcag ggtcatataa
    20281 tccggcgcga tggcggaaag aacgttgcct agcgtgaaaa ttgccatcag gaaaatcaac
    20341 gcactgcggc gggcacgatg agaaagtaga agcgtcatca gcggcgcgcc caccattacg
    20401 ccaactgcat aggcactgat taacattccg gcagcgggaa tcgagacatc cacaccgcgc
    20461 gcaatgacgg gcaacaagcc cattggcgag aactccgttg tcccgatacc aaacgcgcca
    20521 atcgccagcg ccagcaacgg atagttaatt ttcatgcctt atctccacct cttcgcgtca
    20581 ttacgcgata ttcattaaag tggcgaaagc atgacagcaa tcacaaaaaa atgaaaataa
    20641 caaaaagaga aaacactttt gccattttgc taacaaacag gaaggagatg cgagggagaa
    20701 cgcgctccct cgagaggaaa tcagtgcagc gcggcagtca aacccacggc tacgatcaaa
    20761 ccgaggacga taaccgttgt taccagtgaa aatttaaggt cggtgctcat caagttttct
    20821 ccttttttat taccacacaa aaagtgatat tacgcatttt tacacactgt gatgaaaaaa
    20881 tctcccgtca tttataatga taagtgtttt taccacttcc ccttttcgtc aagatcggcc
    20941 aaaattccac gcttacacta tttgcgtact ggccattgac cccttcctga cgctccgtgt
    21001 cgtttttccg gcgtaccgca acacttttgt tgtgcgtaag gtgtgtaaag gcaaacgttt
    21061 accttgcgat tttgcaggag ctgaagttag ggtctggagt gaaatggaat ggcaacaata
    21121 aaagatgtag cgaaacgagc aaacgtttcc actacaactg tgtcacacgt gatcaacaaa
    21181 acacgtttcg tcgctgaaga aacgcgcaac gccgtgtggg cagcgattaa agaattacac
    21241 tactccccta gcgcggtggc gcgtagcctg aaggttaacc acaccaagtc tatcggtttg
    21301 ctggcgacca gcagcgaagc ggcctatttt gccgagatca ttgaagcagt tgaaaaaaat
    21361 tgcttccaga aaggttacac cctgattctg ggtaatgcgt ggaacaatct tgagaaacag
    21421 cgggcttatc tgtcgatgat ggcgcaaaaa cgcgtcgatg gtctgctggt gatgtgttct
    21481 gagtacccag agccgttgct ggcgatgctg gaagagtatc gccatatccc aatggtggtg
    21541 atggactggg gtgaagcaaa agctgacttc accgatgcgg tcattgataa cgcgttcgaa
    21601 ggcggctaca tggccgggcg ttatctgatt gaacgcggtc accgcgaaat cggcgtcatc
    21661 cccggcccgc tggaacgtaa caccggcgca ggccgccttg ccggttttat gaaggcgatg
    21721 gaagaagcga tgatcaaggt gccggaaagc tggattgtgc agggtgactt tgaacctgaa
    21781 tccggttatc gcgccatgca gcaaatcctg tcgcagccgc atcgccctac tgccgtcttc
    21841 tgtggtggcg atatcatggc aatgggcgca ctttgtgctg ctgatgaaat gggcctgcgc
    21901 gtcccgcagg atgtttcgct gatcggttat gataacgtgc gcaacgcgcg ctattttacg
    21961 ccggcgctga ccacgatcca tcagccaaaa gattcgctgg gtgaaacagc gttcaacatg
    22021 ctgttggatc gtatcgtcaa caaacgtgaa gaaccgcagt ccattgaagt gcatccgcgt
    22081 ttgattgaac gccgctccgt ggctgacggc ccgttccgcg actatcgtcg ttaatcaccc
    22141 gttgcgggag tctcttccgg ctcccgcagc cactccttat tcagcgtctc actatcgctg
    22201 agatactcaa gcagccaggt taacgcaggc gacatatcat tttgctgcca tgtcagacaa
    22261 catgccgaat ccgggaaggg gttttccagt tcaagtgcta cccacttccc ctcattaagc
    22321 cacggtttgg cgaaatgtgt tggcaccatc cctatgcata atcctgccga gatacaggtt
    22381 gccgatgatt cccagtcagg tacgacaagt cttttttggt tatccagcaa ccaggtaata
    22441 cgtttaggta gcgttcgcga ggtgtcttcg cgcaccaacg acggccagtt gcgcaacgta
    22501 tcatcgctga acgggccatc catcaacgcc agcgggtggt gactggcaac aacgcaactc
    22561 cagcttagca tccccatatc ccggaaggca taacgaccgc ctaccggaat cgcgcgtgtt
    22621 gcgccaattg ccagttccac gcgcccgtcg gaaagcgcat cccagacacc gttgaacact
    22681 tcctgaaaga caagaagttc gacatcatca aaatggcgat aaaaatcaac gatcatctgc
    22741 cgtgtacgtt ctggcctgac aatattatcc actgcgatag ctaactgacc gcgccagccg
    22801 ttcgctatct gctgacattg ctggcgggtg atctgcattt ttttgacaac agagcgccct
    22861 tctttgagaa accacgctcc agcagcggtc agctccacat cacggtgccg tcgttcaaag
    22921 agcggtaccg ccagccactc ttccagctga cgcacggtat agctgaccgc agaaggaaca
    22981 cgatgcagct cctgtgccgc agcgctaaaa ctaccattac gcgctaccgc atcaacaact
    23041 tcgagtgaat attctgacca catagtctgc ctgcaaaatt tttgaaacca gtcatcaaat
    23101 attaccgttt cacaacacta atttcactcc ctacactttg cggcggtgtt taattgagag
    23161 atttagagaa tatacatgca acctgggaaa agatttttag tctggctggc gggtttgagc
    23221 gtactcggtt ttctggcaac cgatatgtat ctgcctgctt tcgccgccat acaggccgac
    23281 ctgcaaacgc ctgcgtctgc tgtcagtgcc agccttagtc tgttccttgc cggttttgcc
    23341 gcagcccagc ttctgtgggg gccactctcc gaccgttatg gtcgtaaacc ggtgttatta
    23401 atcggcctga caatttttgc gttaggtagt ctggggatgc tgtgggtaga aaacgccgcc
    23461 acgctgctgg tattgcgttt tgtacaggct gtgggggtct gcgccgcggc ggttatctgg
    23521 caagcattag tgacagatta ttatccttca cagaaagtca accgtatttt tgcgaccatc
    23581 atgccgctgg tgggtctgtc tccggcactg gctcccctgt taggaagctg gctgctggtc
    23641 catttttcct ggcaggcgat tttcgccacc ctgtttgcca ttaccgtggt gctgattctg
    23701 cctattttct ggctcaaacc cacgacgaag gcccgtaaca atagtcagga tggtctgacc
    23761 tttaccgacc tgctacgttc taaaacctat cgcggcaacg tgctgatata cgcagcctgt
    23821 tcagccagtt tttttgcatg gctgaccggt tcaccgttca tccttagtga aatgggctac
    23881 agcccggcag ttattggttt aagttatgtc ccgcaaacta tcgcgtttct gattggtggt
    23941 tatggctgtc gcgccgcact gcagaaatgg caaggcaagc agttattacc gtggttgctg
    24001 gtgctgtttg ctgtcagcgt cattgcgacc tgggctgcgg gcttcattag ccatgtgtcg
    24061 ctggtcgaaa tcctgatccc attctgtgtg atggcgattg ccaatggcgc gatctaccct
    24121 attgttgtcg cccaggcgct gcgtcccttc ccacatgcaa ccggtcgcgc cgcagcgttg
    24181 cagaacactc tgcaactggg tctgtgcttc ctcgcaagtc tggtagtttc ctggcttatt
    24241 agtatcagca cgccattgct caccaccacc agcgtgatgt tatcaacagt agtgctggtc
    24301 gcgctgggtt acatgatgca acgttgtgaa gaagttgact gcccgaatca tggcaatgcc
    24361 gaagtcgctc atagcgaatc acactgatct atatcgatat acttatactt aggctgctaa
    24421 caaaattttg ttgtatgatt gaaattagcg gcctatacta atttcgagtt gttaaagcta
    24481 cgataaatat tatgttttta cggggacagg atcgttcccg actcactatg gatggtcatt
    24541 tcggcaaggg ttcctccttt ccctctgttc tacgtcggat tatagactcg cggttttttc
    24601 tgcgagattt ctcacaaagc ccaaaaagcg tctacgctgt tttaaggttc tgatcaccga
    24661 ccagtgatgg agaaactatg agttcatcgt gtatagaaga agtcagtgta ccggatgaca
    24721 actggtaccg tatcgccaac gaattactta gccgtgccgg tatagccatt aacggttctg
    24781 ccccggcgga tattcgtgtg aaaaaccccg atttttttaa acgcgttctg caggaaggct
    24841 ctttggggtt aggcgaaagt tatatggatg gctggtggga atgtgaccga ctggatatgt
    24901 tttttagcaa agtcttacgc gcaggtctcg agaaccaact cccccatcat ttcaaagaca
    24961 cgctgcgtat tgccggcgct cgtctcttca atctgcagag taaaaaacgt gcctggatag
    25021 tcggcaagga gcattacgat ttgggtaatg acttgttcag ccgcatgctt gatcccttca
    25081 tgcaatattc ctgcgcttac tggaaagatg ccgataatct ggaatctgcc cagcaggcga
    25141 agctcaaaat gatttgtgaa aaattgcagt taaaaccagg gatgcgcgta ctggatattg
    25201 gctgcggctg gggcggacta gcgcactaca tggcatctaa ctatgacgta agcgtggtcg
    25261 gcgtcaccat ttctgccgaa cagcaaaaaa tggctcagga acgctgtaaa ggcctggatg
    25321 tcaccatttt gctgcaagat tatcgtgacc tgaacgacca gtttgatcgt attgtttctg
    25381 tggggatgtt cgagcacgtc ggaccgaaaa attacgatac ctattttgcg gtggtggatc
    25441 gtaatttgaa accggaaggt atattcctgc tccatactat cggttcgaaa aaaaccgatc
    25501 tgaatgttga tccctggatt aataaatata tttttccgaa cggttgcctg ccctctgtac
    25561 gccagattgc tcaatccagc gaaccccact ttgtgatgga agactggcat aacttcggtg
    25621 ctgattacga tactacgttg atggcgtggt atgaacgatt cctcgccgca tggccagaaa
    25681 ttgcggataa ctacagtgaa cgctttaaac ggatgtttac ctattatctg aatgcctgcg
    25741 caggtgcttt ccgcgcccgt gatattcagc tctggcaggt ggtgttctca cgcggtgttg
    25801 aaaacggcct tcgagtcgct cgctaaaggc tattctatcg ccccctcttc gggggcgagt
    25861 tcagatcagg cttctgtacc tggttgattc atggcatttt ctcgtgccgc cagcacacgt
    25921 tctaccgtat ctaccactgc ctgagtttgt ggatcgattt caatgttgac gcgtgcgcca
    25981 agttttttct tcccaagagt cgtgcgttcc agtgtttccg gaattaaatg gacgcaaaaa
    26041 cgcgttggcg tgacttcgcc gacggtcagg ctaataccgt cgatgccaat aaatcctttg
    26101 tacaggatat atttcatcaa ctgactatcc tggactttaa accagatctg gcgattattt
    26161 tccgaggtta atattttcgc cacttcagcg gtggtcataa tatgacctga cattaagtgt
    26221 ccgccaattt catcactgaa tttcgccgca cgctcaacgt ttacccaatc ccccactttt
    26281 aaatcgccaa gattggtaat gcgtaacgtt tctttcatca ggtcaaaact gacatggttg
    26341 ccgttaattt ccgtcacggt caggcagcaa ccgttatgcg ccacggaagc accggtttcc
    26401 aggccgtcca gcatgtggtc gggtaactcc accacatgcg tacgaaaatt tggtttctcg
    26461 tcaatcgaca ccagttttgc ggtgccctgt acaatccccg taaacatact tacaactcct
    26521 gaaatcagtt aagacattct gttcagcaca atagcaggtg gaaaacgccc ttaccagtga
    26581 aggggtaaga atggctattt tttcactgga gaattaataa atcctcgcta caatagactg
    26641 aatttcccct gcttcttctt tttgctgccc attcaggcgg ctttttagtc tctcatataa
    26701 ctacaaataa aaggtgttca cgtgcagaag tatatcagtg aagcgcgtct gttattagca
    26761 ttagcaatcc cggtgattct cgcgcaaatc gcccaaactg cgatgggttt tgtcgatacc
    26821 gtgatggcgg gcggctatag tgccaccgac atggcggcgg tcgctatcgg tacttctatc
    26881 tggcttccgg cgatcctctt tggtcacgga ctgctgctgg cattaacgcc ggttatcgcg
    26941 caattaaatg gttccggtcg acgtgagcgc attgcgcatc aggtgcgaca aggtttctgg
    27001 ctggcaggtt ttgtttccgt tctcattatg ctggtgctgt ggaatgcagg ttacattatc
    27061 cgctacatgg aaaacatcga tccggctctg gcggacaaag ccgtgggtta tctgcgtgcg
    27121 ttgttgtggg gcgcgccggg atatctgttc ttccaggttg cccgtaacca atgtgaaggt
    27181 ctggcaaaaa ccaagccggg tatggtaatg ggctttatcg gcctgctggt gaacatcccg
    27241 gtgaactata tctttattta tggtcatttc ggtatgcctg agctcggtgg cgttggttgt
    27301 ggcgtggcta ctgcggcggt gtattgggtc atgttccttg ccatggtttc ttacattaaa
    27361 cgcgcccgct ccatgcgcga tattcgtaac gaaaaaggca ccgctaagcc cgagcctgcg
    27421 gttatgaaac gactgattca actcggtttg ccgattgcgc tggcactgtt ctttgaagtg
    27481 acactgtttg ccgtcgtggc tctgttagtg tctccgctcg gtattgttga tgtcgcagga
    27541 caccagattg ccctgaactt tagttcactg atgtttgtgc ttccaatgtc gctggcggca
    27601 gcggtaacta tccgcgtagg ttatcgtctg ggtcagggct caacgctgga tgcgcaaacc
    27661 gctgcgcgga ccgggcttat ggtgggtgtc tgtatggcaa ccctgacggc cattttcacg
    27721 gtttcactgc gggagcaaat cgccctgttg tacaacgaca atcccgaggt tgtaacgctg
    27781 gctgcgcatt tgatgttgct ggcggcggta tatcagattt ctgactcaat ccaggtgatt
    27841 ggcagtggga ttttgcgtgg ttataaagat acgcgttcca ttttctatat cacctttaca
    27901 gcttactggg tgctgggctt gccaagcggc tatattctgg cactgaccga tctggtcgtt
    27961 gaacctatgg ggccagcagg cttctggata ggctttatta tcggcctgac atcggcagcc
    28021 attatgatga tgctgcgtat gcggttcctg caacgtatgc cgtcagccat cattctgcaa
    28081 cgagcgtccc gctaataaag acaaggcgca accttcacgg gttgcgcctg tgtttttacg
    28141 caggctggag cgttgcgcca atcccgtctt cgtctggctg taatttcaga gcgttacaca
    28201 gagtttgccg actggcatct gttatctcaa cgcgtatatc cccgttaact tcccctttca
    28261 cattgatgaa gtcaggtgaa atttctttcg cactggtttg caggcaaatc gctccctctc
    28321 cgcgcaggcg tttcactttc agttgcacat gcgttgccgc accagtgatc agccacagtt
    28381 ttccgccatt cagattgatg ttaccaacaa tgaaagtgac gctgtcacct tttaccgtac
    28441 tgccgtcgag aatcaacaca ccgccctgct gtacagtaat cgttgattgt tgcagctctg
    28501 cgccataacc aataatcatt gaagcgccgt ccagtaatgc aacactcccg gtcagttcaa
    28561 ccggtgcgcg atcttcctct acgacaaaat tttcgtccgc gctggtgggt gacaacagga
    28621 tcactgttcc accttgtatt accgcatttt ccagcgtacc ggcatgcact tcagcccgcc
    28681 cccccacatt caccgacagg ttttgcgtct taccggaact gtaaagctga aggccatccg
    28741 tccccagacg ataaatggat ccattctcaa caatagtgtc agagtcttca ccaccgagat
    28801 tattcatggt gccaccagca agcaacgtat cactggcttt cccggccagc ataaaaacgc
    28861 cgccctgatt aatctcacaa ttgctggctt ctccacttgg atacaccaac aattcaccac
    28921 cttcatctac cacgaccgct gaggttgtcc cattaaccaa caggccgccc tcttgatcga
    28981 gaatgatttt ttccgcgcga tgtccttcca gtacacgcag gttaccgcca ttttccagca
    29041 acaaaccgca ggcataaccc tgatcaacgc tgaattcacc ttcgggatgg cggccattaa
    29101 ccgtagcgag cgtagagaga ctaattgcgc ctccctcgga ctgaacaata tctgtggcaa
    29161 tggcaccgtc gctgaccgat agcgtgccat tgttaccgta ctgtgtgcat ttggcgatcc
    29221 caccggttgc cactttcaac cagccagccc ccgtgaccac ggtattttcg gctcttccgc
    29281 cagcttcaac aatcaactcg ccttcagtaa gttgcgtcgc gccaaggacc tcgccggtct
    29341 taacaatttg cacattcccg tcgctcatca agtttttcgc atcagatccc atgatttatt
    29401 cctttgctgc atctgtgtgc ctttattgct acctaagtgt aaaggctacg gaggatttat
    29461 ccacgacaga tttgagatgg tggcaaacaa ctctgtttaa actctgatac acgaattatt
    29521 gggttgtatc agatgtaaat gcgatcctga ataaaaatcg cccttgcaaa tcaacaaaat
    29581 atagccaatt tgcttaaaag ctctgcaaac ggtcgaaata gcgcagaaaa ttacgttttg
    29641 cctcttgcca cctttccact ctgccgctaa tattcgtccc cgttgtcacc tacaacgttg
    29701 cgttcatagc tcagttggtt agagcaccac cttgacatgg tgggggtcgt tggttcgagt
    29761 ccaattgaac gcaccatcct gcgtccgtag ctcagttggt tagagcacca ccttgacatg
    29821 gtgggggtcg gtggttcgag tccactcgga cgcaccagat tttcttaatc tggtcttttc
    29881 ctttttccct ctgtttcttc tctgtatcca atacgttaaa agatttacac tgtcttcgta
    29941 tgcgttatca gaaggagaat cgctatggca actttgttac aacttcattt tgcttttaat
    30001 ggcccatttg gcgacgcaat ggctgagcag cttaaaccac ttgctgagtc gattaatcag
    30061 gaacctggtt ttctgtggaa ggtatggaca gaaagtgaaa agaaccacga agccggtggg
    30121 atctaccttt tcactgatga aaaaagcgcc cttgcctatc tggaaaaaca tactgcccga
    30181 ctgaaaaatc tcggcgttga ggaagttgtc gccaaagttt tcgatgtcaa tgagccactt
    30241 agtcaaatca atcaggcaaa actcgcctga cagaatttaa tcaagggcgg ttagcgccct
    30301 tttcatccct gtctgaaatt tctcaaattc taaaaatctc aaccaaactt atctgataac
    30361 actaaattcg aaagaatgcg tacaggtaag taacaatgaa aaaaattgct attgtgggtg
    30421 ccgggcctac ggggatctac accttattct cgcttctaca gcaacaaact ccactttcta
    30481 tttctatctt cgagcaggct gacgaggccg gtgtcgggat gccatacagt gatgaggaaa
    30541 actcaaaaat gatgctggca aatattgcca gtattgaaat accgccgatt aattgtacgt
    30601 atctcgaatg gctacaaaag caagaagcca gccatctcca gcgttatggc gttaaaaaag
    30661 aaaccttgca cgatcgtcag tttttaccgc gaattctgct gggcgaatat ttccgcgatc
    30721 aatttttacg attagtagac caggcacgac agcaaaaatt tgcagtggct gtttatgaat
    30781 catgccaggt taccgatctg caaattacaa atgctggcgt catgctcgct acaaatcagg
    30841 atttacccag cgagacgttt gatttagcgg tgattgccac gggtcacgtc tggcctgatg
    30901 aagaagaagc aaccagaacg tattttccca gcccgtggtc aggcctgatg gaagcaaagg
    30961 tcgatgcgtg taacgtgggt attatgggaa catccttgag cggactggat gcggcaatgg
    31021 cagtggctat tcagcatggt tcgttcattg aagatgataa acaacacgtc gtttttcacc
    31081 gcgataacgc aagtgaaaag ctaaatatta cgttgatgtc gcgcacgggt attttacccg
    31141 aagccgattt ctattgccct attccctacg agcccttaca catcgtcacc gatcaggcat
    31201 taaatgctga gattcaaaaa ggtgaagagg gccttttgga tcgggtattt agattgatag
    31261 tagaggaaat caagtttgct gatccagact ggagccaacg catagcctta gagagcctga
    31321 atgtcgattc ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac ccatttgact
    31381 gggcagaaaa aaatctccag gaagtcgaac gcaataaacg agaaaaacat actgttccct
    31441 ggcgttatgt cattctgcgc ctgcatgaag ccgtacagga aattgttcct catctgaatg
    31501 aacacgacca taaacggttc agtaaaggcc ttgccagggt tttcatcgat aattatgcgg
    31561 caatcccttc agagtctatt cgtcgactac tggccttacg tgaagcgggg atctgaaatc
    31621 cctgatgttg gcgaagatta tacgttacag caacccgaag atattcgtgg acgcgtagcg
    31681 ttcggcgcgt tgccctggtt gatgcacgac cagcctttcg ttcagggact tacggcatgt
    31741 gcagaaattg gtgaggcgat ggctcgggcg gtcgtaaagc ctgcatcccg tgctcgtcgg
    31801 cgtctttcgt ttgattaaag gtaaagctat cagcactcaa caaactgatg ctgttcatta
    31861 agacgatagc ggactccggt tcgaatattg ttttcacctt caatggcgac ggcgaagcga
    31921 actctttcgc cgtcatgata tgccagtgcg gcgcttcccc ctagcccgag aataattgag
    31981 tcgaccacgc ccgtgctcac taccgttgaa ttttcaccgc tggcaatgat gtgtacattg
    32041 tcgccgctgt tggcaattct ggcgtttgcg ccaaagctgg cgatttgcac caggtcacca
    32101 ttactggcga catgacaccg ttcacccaat gtacaaacgc gtactcgcat tccggtattc
    32161 gcaatccgac tactatctcc agcgctgctt atccgcgaag aattaccggt tacagcaacc
    32221 cgtgcgcgtt caccggagct gccaatgtga ctgttaaagc cgacacttcc aatccgcacg
    32281 ctataaccca tactggcaat ttgcgcggca tacccagcgg ttgcgattcg ggcgttatcc
    32341 tcacaacagc cgatgcgagc atgatcgtcg ctgcgggcaa actgactgca aaatggtatc
    32401 tctccggggt ctgtggccag tttttgcata gaggaaactt cctgatgcgc gaagttttca
    32461 tccgccagcc attttttcca cgcatactca accagactct ccgcccagtc cgtatatcct
    32521 tcctcaatca gtgcatgacg aatatcggca taactaccgc cgcgtgggaa atggcgcagg
    32581 aaccagcgat acatcaccgc tccgatacgc cattctcgca ggtctgctcg tgtaatgatc
    32641 attcgttagt gccggtgata gccatcgacc atgcttttaa aggtttcgtg tggcgtacgc
    32701 cccgtggtgc aaagataaag atgaccgaag ataaaaaaga ggcttataaa tgccagagca
    32761 aaatgcgcct gcaataacca gtatcttacg ccaggaaaca catctcccac ggcttgcgga
    32821 tagagacaca acagccccgt caatagtaac aacggcagca atccatacat gacaccaaca
    32881 taggcgacct gctgtaaggg attaaattta gactgtgttg ttgccgggaa aggatgttct
    32941 tccccctgca taatgccaaa caaataaaat cgcgtttgtt ttgccgctcg ttccagccac
    33001 ccctgacgac gaatgcgata gtggtgacca ttatccccaa cggcattgat cagcacaaag
    33061 ccgagccagc atgccagtaa caaaaatccg caaacttcat gcaccgcaac cagactttta
    33121 accgcagttg cgcccaccat cgcaaagtga tttatcagcc cacttgccag caacaataca
    33181 aagagtaacg cattcgacca gtgccataat ctgaccgctt tgctgtaaag ataaaccttc
    33241 tcaccatgat cggtggctga tttcttcacg ccacgagcac gaagcaaggc gtgcagcccc
    33301 agcaccaacc acatcccaac cagcaatact ccggcaatga tcaaccacac cggccaaaat
    33361 tcgggagtga actgtggcac atagttcgct aactggctct gaaactgttc agcatgttgc
    33421 gacgggttca tacattttcc tttttaatca aatgttgacc gaaccggcga tacagatgcg
    33481 gttttccggc accaggtagc tgatattgat agtatttatt atcctgcaac cacgcctgaa
    33541 tttctgggct atcttcgcga ccaaagatca atgcgtgttc cggacaggcg ctgacgcaaa
    33601 tgggcggaaa gccttttgcc agtcgggact cagcgcagaa atcacattta tctgccactt
    33661 tagtgacagg gttaaggtag cgaaccaggt acggacaagc gccaatgcaa taactgcagc
    33721 cgatgcactg cgatttttct acccgcacga tcccctgctc atcacgccac gatgcccccg
    33781 tcgggcatac gtcgatgcac ggagcatctt cgcagtgctg acatgactgg cgaaaaaagt
    33841 gatactgcgt ttcgttgtcg ttatcggtga ctggaatgtg tgctatcgat aaacggcttc
    33901 cctgagcggg gacgtggtta gttttacggc atgcgcgggc gcaaatatta cagccattgc
    33961 accgtgactc gtcatgaatc atggcatacc gaacttcctt ttcttgcctc gtgttcgcta
    34021 acagagaaga cgcagaacca gtaaaaaaga ttactgttcc catccccaga acaaatttgc
    34081 gtcgagtgaa ggacatctga ttatccccta atgcagtggc tgcaactgtc caccctatcc
    34141 atgacatctc cctgaaattt caggggttct gaaactgctc tacctcgaac cattcgcata
    34201 agccataagc cttgccatag gtcataaatc gctcgctaaa caagtacaac accgccgtcg
    34261 atgactccac ctgccgtata tcggcatatt caggtgcaac atccatcgcg gcgatcgcgg
    34321 cttcaatctg cgcttcctgg aagtaatatg gcgcttgcat cagcatggcg actttgtaag
    34381 gacgaggata cgtctggcac tcaaaacgaa cggcgtgtgc aatggcgcga caaatgtcct
    34441 gctccacaac ttgcaaagac atcgccgcgt agttttcact catggcctgc gttgagtaat
    34501 agtaatcatc ctgacttccc gtcatacaag cgatatccgc gtagtctccg ttctgcttaa
    34561 tgctttccag caaaactgac agttcctctt ctgcaaccga atagggtggc gagagaaaca
    34621 cggtgcgcgc caccagctgc ccagccgcag aatgctggcg aatgaactca gccaggattt
    34681 cagcaggttc tggttcggtt accgtatcaa cggggtgacg catcccctgc aaccattgcc
    34741 gcttcgcctc gtcaacttcg ctgactttcg ctaacggcaa ttcatcctga tggttcatct
    34801 tacccattcc ttacgcaggc agtagattgt gtgctgccag atcggcggca atatcttcca
    34861 gccccagacg ctgcaatgtt tcgcgggttg gacaaccaag ctgtgggtcc cagcccattt
    34921 ctttgtagaa catcgtgagc gaagcatgca tatcgtcacg atccattttg tcagtacctt
    34981 cagtaaagac cgggatctgc ggatccttgt cgaaaaccca ggaacagata agatcgtgtt
    35041 cattacgcat atctttggtt tgcatcggtt ttaccgtgta ggcacgatgc aacgtaaaga
    35101 tacgctctgc agctaaatct aatttttcct gagtcatctc ttcgccggtg atcgctttga
    35161 agaatttggc ttcaagcgcc agatcgcccc ggtaattacg gcttttcagc ggcgaaacgg
    35221 tcattggcca gacccagttg cacagagtga cggcgttatg caaacagacc cgcaacagcg
    35281 accactttgc atatttaatt tttgcgtcat tgattggcgt gtagtttttg gtttcatcgt
    35341 aagcatcttc agaaccaaaa agttctttcg ccacttcacg ttgcagtttc aatggcaagc
    35401 cggaaccaat aaagttgata tgggtatgcg tcatgcagtc acggttgaac atacacttaa
    35461 caatggaacc gacctgcgct gacgcttcat tggcatggtg aaccggatag ccaaacggcg
    35521 accagagttt atttttcgcg tagccccagt actctttacc caaattccag cgttctgcga
    35581 tggcatatga accatcagcc aggtgactca gctcacccac acgatgcgcc agacggtagt
    35641 aaaaatcttt aatgaagtta acgtcacccg cttccagttg atcccagcga atttctgcat
    35701 actcttcagc tggcagaaca cgcttgaaca cacctttgct gtaacaataa gtaaagtcgc
    35761 gatgcaactg cccgtagtta caccataggc cgtagtcatc gaacaaattc agaccaacca
    35821 ggttaccaat cacacgacca tcgtctttat cttcaaaatc tttcggcccg ttcgggaaga
    35881 tggtggtatg gacaaagttt gctacacaag tgttaccacc tgtgctgggc acgccaaact
    35941 ctttcacccg aggaatattc atttgggtca tacaacggat cgggcaagag tggcagccgc
    36001 tcatttttac tgtgtatttc tctgccgccg gtcctaagtc aaaaacggat ttataggtac
    36061 gaaagccgac cgtattctga ttgcctggcg gaatttcacc cgtttcaatc ggaccacctt
    36121 cagccgcgcc ccaaaacagc cctttacgtg ctgtccagcg tgacttggga tctgaatact
    36181 ctgcccacga ttgtggcgta cttggcacga catggttgtt attcgcacca ataagttcag
    36241 tcatcatgta atcattgaga cgcttcatct cctgacgatc ggcaatgttg acccctttcg
    36301 tcccttcaac cgcaatcgct ttcaggtttt tcgaacccat tattgcgcca gttcccgcac
    36361 cgccgctgtg gttacggcta ttcaacatgc cagagagagg aataaggttt tccccagcct
    36421 gaccaatagc cgccacacag gtttccgggc tggtcaatcg acaaatttct tccgtcgttg
    36481 cgcgcgtccc ttttccccat aagaaatcgg ctttttccag gctaactttg tcatctttaa
    36541 tcttcagcca taccggtgat ttcgccttcc cttcgataat aatgacgtcg tatccagcga
    36601 atttcatttg cgctgcaaaa aagccaccca tatgggcatc gcgactaaat ttcctttggt
    36661 aaaagtagaa agtgaggtga tatttacgcg agaactacag ggggcaccag atccaggtaa
    36721 tgggccggta gcaaagacta atttattcgc ttcatcgaaa ggtttcgtgc ctagcggtac
    36781 ttcgtcatac ataattttgt agccgaagcc catgccaccg acaaaacttt taaacttact
    36841 ggaatcttcg agggtaatat ttcctgtcgt gagattgact cttaatatat tacctgtcca
    36901 accgttagcc atgatttttt cctttgcaag attcacacag taatatcttt ccactcgata
    36961 atttttaacg cccctgttgg gcaggcgttt gcgcattcac cgcataacac acatttcgag
    37021 gattttttac tttcggtatt tacggtggcc atcatccacg ggcatgccgt agtacaggcg
    37081 ctacagccaa tacaacgttt atgatcgacg gtaatacagc cttctttctg ctgccaggta
    37141 atcgcaccaa tcgggcagac gttcatgcat tgcggctctt tgcattgacg gcaggtgtcc
    37201 gcggtatagt tgagatcgcc atacaggccg ccgccagagc caaccccgtt gtcgccaaag
    37261 aaataattgc gatggatttt gatacgggag aagaatgtcc ctactgagcc atcgttgaag
    37321 ttggtacaag agatttcaca gcggtggcaa cccgtgcatc gcgctcgctg ggtcaccagc
    37381 acccctttgg gtgtatttat caaccctact gtgccgctat caatatcttc ctgtttgcaa
    37441 cccaaaagtg acagtaacgc aggggcaatt gttagaccag caaggccttt tcctgatata
    37501 cgcaagaatt ctaatcgcgt taagccaata tctaatagtg gacgatcaac cgggttcatt
    37561 tatttcgcat cctcttcgca aacacagtgc tgacaattca gcagcctgat attaagcatt
    37621 caactaatac gctgctgact ctgtccccaa caccagatat tttccctaac ccgatagtgt
    37681 atgagaaaag atttaacgcc gataaactaa tcctttatgg ttaggagaaa aaataatgtg
    37741 attattctga gcccttaaca ttgatcgtta tcaatt
//
LOCUS       DAZPEU010000021        36587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPEU010000021 DAZPEU010000000
VERSION     DAZPEU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.021"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHU2L_000954"
     CDS             <1..190
                     /locus_tag="ACHU2L_000954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1355916.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACHU2L_000955"
     CDS             complement(156..1391)
                     /locus_tag="ACHU2L_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1355917.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACHU2L_000956"
     tRNA            complement(1538..1614)
                     /locus_tag="ACHU2L_000956"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACHU2L_000957"
     tRNA            complement(1657..1743)
                     /locus_tag="ACHU2L_000957"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACHU2L_000958"
     tRNA            complement(1764..1839)
                     /locus_tag="ACHU2L_000958"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACHU2L_000959"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHU2L_000959"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACHU2L_000960"
     CDS             complement(2077..3462)
                     /locus_tag="ACHU2L_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1355918.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHU2L_000961"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHU2L_000961"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR1355919.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHU2L_000962"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHU2L_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR1355920.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHU2L_000963"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHU2L_000963"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR1355921.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHU2L_000964"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHU2L_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR1355922.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHU2L_000965"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHU2L_000965"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR1355923.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHU2L_000966"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHU2L_000966"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR1355924.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHU2L_000967"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHU2L_000967"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1355925.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHU2L_000968"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHU2L_000968"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1355926.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHU2L_000969"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHU2L_000969"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR1355927.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHU2L_000970"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHU2L_000970"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR1355928.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHU2L_000971"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHU2L_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR1355929.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHU2L_000972"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHU2L_000972"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR1355930.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHU2L_000973"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHU2L_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR1355931.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHU2L_000974"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHU2L_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR1355932.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHU2L_000975"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHU2L_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR1355933.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHU2L_000976"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHU2L_000976"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR1355934.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACHU2L_000977"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACHU2L_000977"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR1355935.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHU2L_000978"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHU2L_000978"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR1355936.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHU2L_000979"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHU2L_000979"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR1355937.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHU2L_000980"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHU2L_000980"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR1355938.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHU2L_000981"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHU2L_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR1355939.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHU2L_000982"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHU2L_000982"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR1355940.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHU2L_000983"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHU2L_000983"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR1355941.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHU2L_000984"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHU2L_000984"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR1355942.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHU2L_000985"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHU2L_000985"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR1355943.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHU2L_000986"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHU2L_000986"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR1355944.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHU2L_000987"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHU2L_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR1355945.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHU2L_000988"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHU2L_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR1355946.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="ACHU2L_000989"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="ACHU2L_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHU2L_000990"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHU2L_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR1355947.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHU2L_000991"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHU2L_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR1355948.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(36398..36473)
                     /locus_tag="ACHU2L_000992"
     tRNA            complement(36398..36473)
                     /locus_tag="ACHU2L_000992"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="ACHU2L_000993"
     tRNA            complement(36482..36558)
                     /locus_tag="ACHU2L_000993"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8939 a        10012 c         9217 g         8419 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gatgtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAZPEU010000022        35620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPEU010000022 DAZPEU010000000
VERSION     DAZPEU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.022"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHU2L_000994"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHU2L_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR1355949.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="ACHU2L_000995"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="ACHU2L_000995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR1355950.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="ACHU2L_000996"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="ACHU2L_000996"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR1355951.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="ACHU2L_000997"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="ACHU2L_000997"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR1355952.1"
                     /translation="MKTLLIIDANLGQARAYMVKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="ACHU2L_000998"
     CDS             5718..6659
                     /locus_tag="ACHU2L_000998"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR1355953.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="ACHU2L_000999"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="ACHU2L_000999"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR1355954.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="ACHU2L_001000"
     CDS             7679..8929
                     /locus_tag="ACHU2L_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1355955.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /locus_tag="ACHU2L_001001"
                     /pseudo
     CDS             complement(9000..9694)
                     /locus_tag="ACHU2L_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="ACHU2L_001002"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="ACHU2L_001002"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR1355956.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="ACHU2L_001003"
     CDS             10866..12116
                     /locus_tag="ACHU2L_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1355957.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="ACHU2L_001004"
     CDS             complement(12172..13260)
                     /locus_tag="ACHU2L_001004"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1355958.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHU2L_001005"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHU2L_001005"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR1355959.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="ACHU2L_001006"
     CDS             complement(14194..15243)
                     /locus_tag="ACHU2L_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR1355960.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /gene="yieE"
                     /locus_tag="ACHU2L_001007"
     CDS             15342..16223
                     /gene="yieE"
                     /locus_tag="ACHU2L_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR1355961.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="ACHU2L_001008"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="ACHU2L_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR1355962.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="ACHU2L_001009"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="ACHU2L_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHU2L_001010"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHU2L_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="ACHU2L_001011"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="ACHU2L_001011"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR1355963.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHU2L_001012"
     CDS             21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHU2L_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR1355964.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="ACHU2L_001013"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="ACHU2L_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR1355965.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="ACHU2L_001014"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="ACHU2L_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR1355966.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="ACHU2L_001015"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="ACHU2L_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR1355967.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="ACHU2L_001016"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="ACHU2L_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR1355968.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHU2L_001017"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHU2L_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHU2L_001018"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHU2L_001018"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR1355969.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="ACHU2L_001019"
     CDS             complement(30809..31048)
                     /locus_tag="ACHU2L_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR1355970.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="ACHU2L_001020"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="ACHU2L_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR1355971.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="ACHU2L_001021"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="ACHU2L_001021"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR1355972.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="ACHU2L_001022"
     CDS             complement(32934..33629)
                     /locus_tag="ACHU2L_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR1355973.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="ACHU2L_001023"
     CDS             complement(33626..34024)
                     /locus_tag="ACHU2L_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR1355974.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="ACHU2L_001024"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="ACHU2L_001024"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR1355975.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         8753 a         8695 c         9418 g         8754 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggtga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg
    34921 cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc
//
LOCUS       DAZPEU010000023        34967 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPEU010000023 DAZPEU010000000
VERSION     DAZPEU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.023"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..783)
                     /locus_tag="ACHU2L_001025"
     CDS             complement(<1..783)
                     /locus_tag="ACHU2L_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1355976.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..961
                     /locus_tag="ACHU2L_001026"
                     /pseudo
     CDS             875..961
                     /locus_tag="ACHU2L_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(971..1051)
                     /locus_tag="ACHU2L_001027"
                     /pseudo
     CDS             complement(971..1051)
                     /locus_tag="ACHU2L_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1366..1842)
                     /locus_tag="ACHU2L_001028"
     CDS             complement(1366..1842)
                     /locus_tag="ACHU2L_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR1355977.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="ACHU2L_001029"
     CDS             complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="ACHU2L_001029"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR1355978.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3277..4317
                     /gene="bioB"
                     /locus_tag="ACHU2L_001030"
     CDS             3277..4317
                     /gene="bioB"
                     /locus_tag="ACHU2L_001030"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR1355979.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4314..5468
                     /gene="bioF"
                     /locus_tag="ACHU2L_001031"
     CDS             4314..5468
                     /gene="bioF"
                     /locus_tag="ACHU2L_001031"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR1355980.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5455..6210
                     /gene="bioC"
                     /locus_tag="ACHU2L_001032"
     CDS             5455..6210
                     /gene="bioC"
                     /locus_tag="ACHU2L_001032"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR1355981.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6203..6880
                     /gene="bioD"
                     /locus_tag="ACHU2L_001033"
     CDS             6203..6880
                     /gene="bioD"
                     /locus_tag="ACHU2L_001033"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1355982.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7459..9480
                     /gene="uvrB"
                     /locus_tag="ACHU2L_001034"
     CDS             7459..9480
                     /gene="uvrB"
                     /locus_tag="ACHU2L_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR1355983.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="ACHU2L_001035"
     CDS             complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="ACHU2L_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR1355984.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10845..10989
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10977..11966
                     /gene="moaA"
                     /locus_tag="ACHU2L_001036"
     CDS             10977..11966
                     /gene="moaA"
                     /locus_tag="ACHU2L_001036"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR1355985.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11988..12500
                     /gene="moaB"
                     /locus_tag="ACHU2L_001037"
     CDS             11988..12500
                     /gene="moaB"
                     /locus_tag="ACHU2L_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR1355986.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12503..12988
                     /gene="moaC"
                     /locus_tag="ACHU2L_001038"
     CDS             12503..12988
                     /gene="moaC"
                     /locus_tag="ACHU2L_001038"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR1355987.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12981..13226
                     /gene="moaD"
                     /locus_tag="ACHU2L_001039"
     CDS             12981..13226
                     /gene="moaD"
                     /locus_tag="ACHU2L_001039"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR1355988.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13228..13680
                     /gene="moaE"
                     /locus_tag="ACHU2L_001040"
     CDS             13228..13680
                     /gene="moaE"
                     /locus_tag="ACHU2L_001040"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR1355989.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13817..14521
                     /locus_tag="ACHU2L_001041"
     CDS             13817..14521
                     /locus_tag="ACHU2L_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1355990.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14726..15439
                     /locus_tag="ACHU2L_001042"
     CDS             14726..15439
                     /locus_tag="ACHU2L_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1355991.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15475..16431)
                     /locus_tag="ACHU2L_001043"
     CDS             complement(15475..16431)
                     /locus_tag="ACHU2L_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR1355992.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="ACHU2L_001044"
     CDS             complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="ACHU2L_001044"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR1355993.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17669..18430)
                     /locus_tag="ACHU2L_001045"
     CDS             complement(17669..18430)
                     /locus_tag="ACHU2L_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1355994.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18563..18973
                     /locus_tag="ACHU2L_001046"
     CDS             18563..18973
                     /locus_tag="ACHU2L_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR1355995.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18935..20041)
                     /locus_tag="ACHU2L_001047"
     CDS             complement(18935..20041)
                     /locus_tag="ACHU2L_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1355996.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20052..21185)
                     /locus_tag="ACHU2L_001048"
     CDS             complement(20052..21185)
                     /locus_tag="ACHU2L_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1355997.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21178..22914)
                     /locus_tag="ACHU2L_001049"
     CDS             complement(21178..22914)
                     /locus_tag="ACHU2L_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1355998.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="ACHU2L_001050"
     CDS             complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="ACHU2L_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR1355999.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="ACHU2L_001051"
     CDS             complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="ACHU2L_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR1356000.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24805..26025
                     /gene="rhlE"
                     /locus_tag="ACHU2L_001052"
     CDS             24805..26025
                     /gene="rhlE"
                     /locus_tag="ACHU2L_001052"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR1356001.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(26019..26117)
                     /locus_tag="ACHU2L_001053"
                     /pseudo
     CDS             complement(26019..26117)
                     /locus_tag="ACHU2L_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="ACHU2L_001054"
     CDS             complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="ACHU2L_001054"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR1356002.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26801..28951
                     /gene="dinG"
                     /locus_tag="ACHU2L_001055"
     CDS             26801..28951
                     /gene="dinG"
                     /locus_tag="ACHU2L_001055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR1356003.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28979..29941
                     /gene="ybiB"
                     /locus_tag="ACHU2L_001056"
     CDS             28979..29941
                     /gene="ybiB"
                     /locus_tag="ACHU2L_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR1356004.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30082..31167
                     /gene="hcxB"
                     /locus_tag="ACHU2L_001057"
     CDS             30082..31167
                     /gene="hcxB"
                     /locus_tag="ACHU2L_001057"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR1356005.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31234..31293
                     /gene="ybiE"
                     /locus_tag="ACHU2L_001058"
     CDS             31234..31293
                     /gene="ybiE"
                     /locus_tag="ACHU2L_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR1356006.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31396..31656)
                     /gene="ybiJ"
                     /locus_tag="ACHU2L_001059"
     CDS             complement(31396..31656)
                     /gene="ybiJ"
                     /locus_tag="ACHU2L_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR1356007.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31921..32187)
                     /gene="ybiI"
                     /locus_tag="ACHU2L_001060"
     CDS             complement(31921..32187)
                     /gene="ybiI"
                     /locus_tag="ACHU2L_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR1356008.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32261..32938)
                     /gene="ybiX"
                     /locus_tag="ACHU2L_001061"
     CDS             complement(32261..32938)
                     /gene="ybiX"
                     /locus_tag="ACHU2L_001061"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR1356009.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32980..34967)
                     /locus_tag="ACHU2L_001062"
                     /pseudo
     CDS             complement(32980..34967)
                     /locus_tag="ACHU2L_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8286 a         8968 c         9446 g         8267 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggc aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtctgccca ccacagcatc atttctctgc
     1021 gcttatcgag atactgagca tggttgtaaa ttcagctaac ccagtgcgtt gacaaaaaat
     1081 tagcgcaaga ggacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca
     1141 tctaagtggt tgattcatag tgactggata tgttgtgttt tgcagtatta tgcagtctat
     1201 tttttaagct aaatgtatta taatgcattg atattaatga tttttactgt ttctcggtca
     1261 gcttttttat actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat
     1321 acctttaagt gataccagat ggcattgcgc catctggcag agtgattaac taaacatcgc
     1381 ggtaatcgat gcgcttgcca gagagtggaa atgaacgtta aacccgacca tcgcgccgct
     1441 ggctccttca tcgacatcaa tacgctctac atccagcgcg tgaacggtaa aaatgtagcg
     1501 atgggtttcg cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa aatcggtacg
     1561 cgtctgcaaa acgccgtctg gcattgctac cagaccagag ccaaaccctt gcggtaatac
     1621 gcgggtatca gcgggtaaat taacaactac ccagtgccac cagccggagc cggttggcgc
     1681 atccgggtcg tagcaggtga caacaaaact tttcgttccc gcaggaacat catcccacgc
     1741 cagatgcggt gaaatattat cgccatcgta acccatgccg ttaaagacat gacgatgcgg
     1801 caacttatcg ccatcgcgca gatcgttact gatgagtttc atgaaccctc ctttcttgtt
     1861 tgcagaaagt gtagccagaa accctcacgc tgacttcccg ttattggcaa aaaaatgttt
     1921 catcctgtac cgcgcggtta accgctgcgg tcagacgctg caactgttgc gggagaataa
     1981 tatagggcgg catcaggtaa atcagtttgc caaaaggccg gatccatacc ccctgttcga
     2041 caaagaattt ttgcagcgcc gccatattca ccggacgagt ggtttcgacc acaccgattg
     2101 cccccagtac gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc agttgctcgc
     2161 gcagctgcac ttcaatagcc gccacctgct gctgccattc gccggattcg ataatcgcta
     2221 ggctggcgtt tgctgccgcg caggccagcg gattgcccat aaaagttggc ccatgcataa
     2281 agcagccggc ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta agtgtggcgg
     2341 aaagggtcat tgtgccgccg gttaaggctt taccgaggca caaaatgtcc ggcgcgattt
     2401 ctgcatattc acaggcaaac agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg
     2461 caatcagcaa gataccttcg cgatcgcaca ttttgcggat tcgttttaac cattccggat
     2521 ggtacatgcg catcccgcct gcgccctgga caatcggctc aatgatcacc gctgcgattt
     2581 catgacgatg cgccgccatc aggcgggcaa aaccaaccat atcgcgttca tcccattcac
     2641 catccatgcg gctttgcggg gcgggagcaa acaagttttc tggcagatag cctttccaca
     2701 ggctgtgcat tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat
     2761 aaccattgcg gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc cagtactgca
     2821 acgccatttt catcgccact tccaccgcta cggaaccgga gtccgcgaga aaaacgcact
     2881 ccagcggttg cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg
     2941 tgataccgcc aaacatcaca tgcgacatgg catcaatttg cgtcttcatc gccgcattaa
     3001 gctgcgggtg attgtagccg tggatcgccg cccaccagga cgacataccg tcaaccaggc
     3061 gtctgccgtc agacaaaatt agctcgcaac cttcggcgct cgccaccgga taaaccggca
     3121 gaggggaggt catggatgtg tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt
     3181 ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt taggtttaca agcctacacc
     3241 gaattaacaa caaaaaacac gttttggaga agccccatgg ctcaccgccc acgctggaca
     3301 ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag
     3361 caggtgcatc gccagcattt cgaacctcgt caggtgcagg tcagcacgtt gctgtcgatt
     3421 aagaccggag cttgtccgga agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc
     3481 gggctggaag ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg
     3541 aaagcggcag gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc ccacgaacgc
     3601 gatatgccgt acctggaaca aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt
     3661 atgacgctgg gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc cgggctggat
     3721 tactacaacc acaacctgga cacctcgccg gagttttacg gcaatatcat caccacacgc
     3781 acttatcagg aacgcctcga tacgctggaa aaagtgcgcg atgccgggat caaagtctgc
     3841 tcaggtggca ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa
     3901 ctggcaaacc tgccgacgcc gccggaaagc gtaccaatca acatgctggt gaaggtgaaa
     3961 ggcacgccgc ttgccgataa cgatgacgtt gatgcctttg attttattcg caccattgcc
     4021 gtagcgcgga tcatgatgcc gacctcttac gtgcgccttt ctgccggacg cgagcagatg
     4081 aacgaacaga ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt ctacggttgc
     4141 aaactgctga ccacgccgaa tccggaagaa gataaagacc tgcaactgtt ccgcaaactg
     4201 gggctaaatc cgcagcaaac tgccgtgctg gcaggggata acgaacaaca gcaacgtctt
     4261 ggacaggcgc tgatgacccc ggacaccgac gaatattaca acgcggcagc attatgagct
     4321 ggcaggagaa aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc
     4381 gttatccggt ggcgcaagga gccggacgct ggctggtggc ggatgatcgc cagtatctga
     4441 acttttccag taacgattat ttaggtttaa gccatcatcc gcaaattatc cgtgcctgga
     4501 agcagggggc ggagcaattt ggcatcggta gcggcggctc cggtcacgtc agcggttata
     4561 gcgtggtgca tcaggcgctg gaagaagaac tggccgagtg gcttggctat tcgcgggcgc
     4621 tgctgtttat ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg atggcgaaag
     4681 aggaccgtat tgttgccgac cggcttagcc atgcctcatt gctggaggct gccagtttaa
     4741 gcccgtcgca gcttcgccgt tttactcata acgatgtcgc tcatctggcg cgactgcttg
     4801 cttccccctg tccggggcag caactggtgg tgacagaagg cgtgttcagc atggacggcg
     4861 atagcgcgcc actggcggaa atccagcagg taacgcaaca gcacaatggc tggttgatgg
     4921 tcgatgatgc ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc tgctggctgc
     4981 aaaaggtaaa accagaattg ctggtagtga cttttggcaa aggatttggc gtcagtgggg
     5041 ctgcggtgct ttgctcaagt acggtggcgg attatctgct gcaattcgcc cgccacctta
     5101 tctacagcac cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg ctggcggtca
     5161 ttcgccgtga tgagggggat gcacggcgcg aaaaactggt gtcactcatt gcgcgttttc
     5221 gtgccggagt acaggatttg ccgtttacgc tggctgattc atgcagcgcc atccagccat
     5281 tgatcgtcgg tgataacagc cgtgcgttac aactggcaga aaaactgcgc cagcaaggct
     5341 gctgggtcac ggcgattcgc ccgccaaccg tacccgctgg tactgcgcga ctgcgcttaa
     5401 cgctaaccgc tgcgcatgaa atgcaggata tcgaccgtct gctggaggtg ctgcatggca
     5461 acggttaata aacaagccat tgcagcggca tttggtcggg cagccgcaca ctatgagcaa
     5521 catgcagatc tacagcgcca gagtgctgac gccttactgg caatgcttcc acagcgtaaa
     5581 tacacccacg tactggacgc gggttgtgga cctggctgga tgagccgcca ctggcgggaa
     5641 cgtcacgcgc aggtgacggc gttagatctc tcgccgccaa tgcttgcgca agcccgccat
     5701 aaagatgccg cagatcatta tctggcagga gatatcgaat ccctgccgtt agcgactgcg
     5761 acgttcgatc ttgcatggag caatctcgcg gtgcagtggt gcggtaattt atccacggca
     5821 ctccgcgagc tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac cacgctggtg
     5881 cagggatcgt tacccgaact gcatcaggcg cggcaggcgg tggacgagcg tccgcatgct
     5941 aatcgctttt taccgccaga tgaaatcgaa gagtcgctga acggcgtgcg ttatcaacat
     6001 catattcagc tcatcacact atggtttgat gatgcgctca gtgccatgcg ttcgctgaaa
     6061 ggcatcggtg ctacgcatct tcatgaaggg cgcgacccgc gaatattaac gcgttcgcag
     6121 ttgcagcgat tgcaactggc ctggccgcaa caacaggggc gatatcctct gacgtatcat
     6181 ctttttttgg gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa cggataccga
     6241 agtggggaaa accgtcgcca gttgtgcact tttacaagcc gcgaaggcag taggttatcg
     6301 gacggcaggt tataaaccgg tcgcctctgg cagcgaaaag accccggaag gcttacgtaa
     6361 tagtgacgcg ctggcgttac agcacaacag cagcctgcag ctggattacg caacggtaaa
     6421 tccttacacc ttcgcagaat ccacctcgcc gcacatcatc agcgcccaag agggcaggcc
     6481 gatagaatca ttggtaatga gcgccggatt acgcgcgctt gaacaacagg ctgactgggt
     6541 gttagtggaa ggtgctggcg gctggtttac gccgctttct gacactttca cttttgcaga
     6601 ttgggtaaca caggaacaac tgccggtgat actggtagtt ggtgtgaaac tcggctgtat
     6661 taatcacgcg atgttgactg cgcaggcaat acaacacgcc ggactgactc tggcgggttg
     6721 ggtggcgaac gatgttacgc ctccgggaaa acgtcacgct gaatatatga ccacgctcac
     6781 ccgcatgatt cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa atccagaaaa
     6841 tgcggcaacc ggaaagtaca taaaccttgc cttgttgtag ccattctgta tttggttaaa
     6901 ttgcgagcga gatcgcgtct tcgattgact gcaatttaac caattaaatt ctaaaataat
     6961 cacgaaaaaa attttacttc cgcctcatgc ggcgaatgtg ggaattgccc aggcggcggg
     7021 ggataggggc tggagacagt tatccactat tcctgtggat aaccatgtgt attagagtta
     7081 gaaaacacga ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt taaagacggc
     7141 tcaaagaaat atcttttatt ttttaactgg ttagataaat gcaatggcag tcactgaaca
     7201 ggcatctctt gccataaaac tgtcatcact catcttgaca aatgttaaaa aagccgttgc
     7261 tttggggata acccggtaag gctggagttt tatctcgcca cagagtaaat tttgctcatg
     7321 attgacagcg gagtttacgc tgtatcagaa atattatggt gatgaactgt ttttttatcc
     7381 agtataattt gttggcataa ttaagtacga cgagtaaaat tacatacctg cccgcccaac
     7441 tccttcaggt agcgactcat gagtaaaccg ttcaaactga attccgcttt taaaccttct
     7501 ggcgatcagc cagaggcgat tcgacgtctc gaagaggggc tggaagatgg cctggcgcac
     7561 cagacgttac ttggcgtgac tggctccggg aaaaccttca ccattgccaa tgtcattgct
     7621 gaccttcagc gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc ccagctgtat
     7681 ggcgaaatga aagagttctt cccggaaaac gcggtggagt atttcgtctc ctactacgac
     7741 tactatcagc cggaagccta tgtaccgagt tccgacacct tcattgagaa agatgcctcg
     7801 gttaacgaac atattgagca gatgcgtttg tccgccacca aagcgatgct ggagcggcgt
     7861 gatgtgattg tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc tgatttatat
     7921 ctcaagatga tgctccatct cacggtcggt atgattatcg atcagcgcgc gattctgcgc
     7981 cgactggcgg agctgcaata cgctcgtaat gatcaagcat tccagcgtgg tactttccgc
     8041 gttcgtggcg aggtgataga tatcttcccg gcagaatcgg atgacattgc acttcgcgtg
     8101 gaactgtttg acgaggaagt ggaacgattg tcgttatttg acccgctgac cgggcagatt
     8161 gtttccacta ttccacgttt taccatctac ccgaaaacgc actacgtcac accgcgcgag
     8221 cgcatcgtac aggcgatgga ggagatcaaa gaagaactgg ccgccagacg caaagtgctg
     8281 ttggaaaaca acaaactgct ggaagagcag cggctgaccc agcgtaccca gtttgatctg
     8341 gagatgatgg acgagctggg ctactgttcg gggattgaaa actactcgcg cttcctctcc
     8401 ggtcgtggac cgggtgagcc accgccgacg ctgtttgatt acctgcctgc cgatgggctg
     8461 ctggtcgtcg atgaatctca cgtcaccatt ccacaaattg gcggcatgta tcgcggtgac
     8521 cgggcgcgta aagagacact ggtggagtac ggcttccgcc tgccatcagc gctggataac
     8581 cgtccgctta agtttgaaga gttcgaagca ttagcgccgc aaaccatcta tgtttcggcg
     8641 acgccgggta attacgagct ggaaaaatcc ggcggcgatg tggtggatca ggtggtgcgt
     8701 ccaaccggat tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca ggttgatgat
     8761 cttctttcgg agattcgtca gcgagcggca attaacgaac gcgtgctggt caccacactg
     8821 accaagcgaa tggcggaaga tcttaccgaa tatctcgaag aacacggtga gcgcgtgcgt
     8881 tatcttcact cagatatcga caccgtcgag cgtatggaga ttatccgcga cttgcgtctg
     8941 ggtgagttcg acgtattggt agggatcaac ttactgcgtg aaggtctgga tatgccggaa
     9001 gtgtcgctgg tggcgatcct cgacgctgac aaagaaggct tcctgcgttc cgaacgttcg
     9061 ttgatccaga ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat tctctacggc
     9121 gataggagca ctccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg ccgtgagaaa
     9181 cagcagaagt acaacgagga acacggcatt acgccgcaag gcttgaacaa gaaagtggtc
     9241 gatatcctgg cgctggggca gaacattgcc aaaaccaaag cgaagggcag aggaaaatcg
     9301 cgcccgattg ttgagccgga taatgtgccg atggatatgt tgccgaaagc gttgcagcag
     9361 aaaatccatg aactggaagg gctgatgatg caacacgcgc agaatctgga gttcgaagaa
     9421 gcggcacaaa ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc cgcatcgtaa
     9481 caggatagcg aagaagactg atgacaaacg gaaaacagtc tgatgcgcta cgcttatcag
     9541 gcctacattt tctccgcaat atattgaatt tgcgcggttt gtaggccgga taaggcgttc
     9601 acgccgcatc cggcataaac aacgcgcact ttgtcttcaa taggaagccg gaattttcct
     9661 tcggatttcc gttaacctaa agcctgtaac gccttttcca gcgcgttatg taacaactgg
     9721 cggtcatgac gatacggaat atcgctggcc tccagtacct cctggatcac aatccgctct
     9781 ttcaccgccg agacatccac ttttggcccg acgatgaccg catcaatgac ctttttacca
     9841 acatactgct ccataattgc cagcttgctt tccagcttca aattagccgc aggtaaactc
     9901 aactcacgcc ccagattgcc gatataaacc atcggcgctg gcgtgcggcg taatgcctgg
     9961 gcgatttcct tcagcagcag aattggcatc aggctggtat aaaaactgcc aggcccaata
    10021 atgatgagat ccgcttcatt gatagcgtga accgcctcac gcgttgcggg tacattaggc
    10081 gttaacaata actcttgaat cggcgtagtt aactggtcga tattgacctc gccgtaaact
    10141 tcatgcccct gatcgtcaat cgccatcaga tcaacaggat gctctgacat tggaatcaaa
    10201 tgcgtatcca ctttcagcag attacgaatt aaattgatgg cttccagagg ccgcacgcta
    10261 aggtgatcca gcgcctttaa catcaagttt ccgagattat gaccggaaag ttcgccattg
    10321 ccgccaaaac ggtattcaaa catcgcggag gcgacgttcg gttccgttat cagctggttg
    10381 aggcagttgc gcatatcgcc ccaagcaatg ccgccttctg aacggcgaat acgccccgtc
    10441 gagccaccat tatccgtggt ggtgacgata cccgttaaac gagaacccaa agacgaaagt
    10501 gatgagagaa cgcgtcccag tccatgccct ccgccgagag caacgacacg atcaagatca
    10561 gccagcgtac gattgcgcat atagtttcct ggagtcagat tatccgcgct acagtagcgc
    10621 aaattgcggg gaaacagcaa ttaacctgcc aatattgagg atacaaacta ttttcttcta
    10681 tctctaaagg acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt aaaaccgcca
    10741 ttttccctta ttctgtagcg aaatagcacg atcatgacgc tatatacatg attacatagc
    10801 gaaagtgtgg atggtaaaaa tctcatttac acgctagtat cggcttaacc actaaacact
    10861 ctagcctctg cacctgggtc aactgatacg gtgctttggc cgtgacaatg ctcgtaaaga
    10921 ttgccaccag ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag gtgtacatgg
    10981 cttcacaact gactgatgta tttgcgcgta agttttacta cttgcgcctg tcgattaccg
    11041 atgtgtgtaa ctttcgttgc acctactgcc tgccggatgg ctacaaaccg agcggcgtca
    11101 ccaataaagg ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc ttcgccagtc
    11161 tgggcaccga aaaagtgcgc ctgacaggag gagagccgtc tttacgccgt gactttaccg
    11221 atatcatcgc cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc accaccaatg
    11281 gttaccgtct ggaacgcgat gtggcgaact ggcgcgatgc gggacttact ggcattaacg
    11341 tcagtgtcga cagtttggac gcccgccagt ttcacgctat taccgggcag gataaattca
    11401 accaggtcat ggcagggatt gatgctgcat ttgaggccgg ttttgagaag gtcaaagtca
    11461 ataccgtgct gatgcgtgat gttaatcatc accagctcga cacctttctg aactggatcc
    11521 agcatcgccc tatccagctg cgtttcatcg aactgatgga aacgggcgag ggcagcgagc
    11581 tcttccgtaa gcatcacatc tctggtcagg ttctgcgtga cgagctactg cgtcgcggct
    11641 ggatccacca attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc catccagatt
    11701 acgccggaga gattggcctt atcatgccgt atgaaaaaga cttctgcgcc acttgcaacc
    11761 gcctgcgcgt ttcctccatt ggtaaactcc atctctgcct gtttggtgaa ggcggcgtta
    11821 acctgcgcga tctgctggaa ggcgataccc agcaacaggc gctggaagcg cgtatttcag
    11881 cggcgctgcg ggagaagaaa cagacccatt tcctgcatca aaacaacacc ggtattacgc
    11941 aaaacttatc gtacattggc ggctaaaacg tcaaaaggag agatcagatg agtcaggtaa
    12001 gcactgaatt tatcccgacc cgtattgcta ttcttacggt ttctaatcgt cgcggtgaag
    12061 aagacgatac ctccggtcac tatctgcgcg attcggcgca agaagcgggc catcacgttg
    12121 tcgatgaagc cattgtgaaa gaaaaccgct acgctattcg cgctcaggta tctgcgtgga
    12181 tcgccagcga cgatgtacaa gtggtattga ttacgggtgg tactggcctg acggaaggtg
    12241 atcaggctcc cgaagcgctg ctgccgttgt tcgaccgtga ggttgaaggt tttggtgaag
    12301 tgttccgtat gttgtcgttt gaagagattg gcacttccac gttgcaatct cgtgcggtag
    12361 cgggcgtcgc taacaaaacg ctgattttcg ccatgccggg ttcgaccaaa gcgtgccgta
    12421 ccgcatggga aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg tgtaatttcc
    12481 atccacattt gaagaaataa gtatgtcgca actgacccat atcaacgccg ctggcgaagc
    12541 gcacatggtg gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg cggaagcctt
    12601 tgtcaccatg cgcagcgaga cgctggcgat gattattgat ggtcgccacc acaaaggcga
    12661 cgtatttgcc actgcgcgta ttgccggtat tcaggcggca aaacgcactt gggatctgat
    12721 cccgctgtgt cacccgctga tgctcagcaa agttgaagtc aatttacagg ccgagccgga
    12781 gcacaatcgg gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg gtgtcgaaat
    12841 ggaagcgtta accgcggcct ccgtggcggc gctgaccatt tatgacatgt gcaaagcggt
    12901 gcaaaaagat atggtgattg gtccggtacg tttgctggcg aagagcggcg gcaagtcggg
    12961 tgactttaag gtggaagcgg atgattaaag ttcttttttt cgcccaggtg cgcgagttgg
    13021 tgggaacaga tgcaaccgaa gtggctgcgg attacccaac cgttgaagcg ttacgccagc
    13081 acctggctgc gcagagcgat cgctgggcgc tggcgctgga agatggcaaa ttgctggctg
    13141 ccgtcaacca gacgctggtg agttttgacc atccgctgac tgacggcgac gaagtagctt
    13201 tcttcccgcc ggtaaccgga ggttaagatg gcagaaacca aaattgttgt cggcccgcag
    13261 ccgttcagcg taggagaaga gtacccgtgg ctggcggagc gtgacgaaga cggtgcggta
    13321 gtcaccttca ccggaaaggt gcgcaaccat aacctgggcg acagcgtcaa agcattaacc
    13381 ctcgaacact atccggggat gactgaaaaa gcactggcag aaattgttga tgaagcgcgt
    13441 aaccgctggc cgctggggcg cgtcacggtg attcaccgca tcggggaatt atggccgggc
    13501 gatgaaatcg tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt tgaagccggg
    13561 cagtttatta tggattatct caaaacccgc gcaccgttct ggaagcgcga agccacgccg
    13621 gaaggcgacc gctgggttga agcccgggag agcgatctgc aggcggcaaa acgctggtag
    13681 ttttttgtta gccggataag gcaccgctgc gtccggcaat ttcaccgctc agtacatgcg
    13741 tttacgctgc gtcgatgcac agcctcatca ttttgcagta tccttaagat attccttata
    13801 tcttcaggag atcgtcatgg acagattccc acgttctgat tcaatcgtac aaccctgggc
    13861 tggcttgcaa acttatatgg cgcaagtcta tggctggatg accgttggct tgttgctgac
    13921 cgcatttgtt gcctggtatg cggctaattc cgcggccgtg atggagctgt tgttcactaa
    13981 ccgtgtcttt ttaatcggtc tgatcatcgc gcaattagca ttggttattg tgttatcagc
    14041 gatgattcaa aagctgagcg caggtgtaac gacgatgctc tttatgcttt attcggcgct
    14101 gacgggtctt acgctttcca gtatattcat tgtctatacc gctgcttcta tcgccagtac
    14161 tttcgtcgtt actgccggga tgttcggcgc aatgagcctg tacggttaca ccacgaagcg
    14221 cgatttaagt ggctttggca atatgctgtt tatggcgtta atcggcattg tgctggcatc
    14281 gctggtcaac ttctggctga aaagcgaagc attgatgtgg gcagttacct acatcggcgt
    14341 gattgtcttt gtcggattga cggcgtatga cactcagaaa ttgaaaaata tgggtgagca
    14401 gattgatacc cgcgacacgt cgaacctgcg caaatattcc attcttggtg cgttaacctt
    14461 gtatctggac ttcatcaacc tgttcctgat gttgttacgg atcttcggca accgccgtta
    14521 attctttttc accatcacta tcccggccat gtgtcgggat ttttttctta ctaaaaacga
    14581 cacttaacaa gtaccaggta taaaatatgc cttatgagcg agcggaatcg ttttccgtat
    14641 ccataaatac aaaagtattt tgtatgcgtg gttttaaatt tattgatggt gaatattaat
    14701 gttttttcta aggacaacta aaagaatgga atcatactcg caaaacagca ataaattaga
    14761 ttttcagcac gaggccagga tattaaacgg tatatggctc attaccgctt taggtttggt
    14821 ggcaaccgca ggactagcct ggggagctaa gtatctcgaa attacggcaa ccaaatatga
    14881 ttcaccacca atgtatgtcg ccatagggtt attattactt tgtatgtatg gcttaagtaa
    14941 ggatatcaac aagataaatg ccgccatcgc gggcgtaata tatctgtttt tactctcttt
    15001 ggtggcgatt gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg tgttcagcac
    15061 tgcgggcgcg atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg
    15121 ttctcaccgt tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa
    15181 tgcggcatta atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgtgttatg
    15241 gccaggcatt atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag
    15301 tgaagagttg tggagtccgg ttcgttgcgc ttttacaggt gcattaacac tctattacta
    15361 ttttatcggc ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca
    15421 tacgcgtttt tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat
    15481 cgccgcttca tttttcgccc gtagcttctt cgcttggctt tccagtagca ggtaacagat
    15541 cagcgccagc agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat
    15601 aatgctccct ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac
    15661 accgatcccc gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc
    15721 cagtacaaag aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac
    15781 catccagtta acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac
    15841 cagcttttgc cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata
    15901 aaccgcgata atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata
    15961 ccagtaatcc ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata
    16021 gcccagccag ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc tgcccggtaa
    16081 ccctagtcgg gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt
    16141 caggttgaag gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg
    16201 accgcagtaa aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac
    16261 cagcccgacc gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc
    16321 ttcccagtcc acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag
    16381 ccaggtgagg atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcaggggttt
    16441 acccccgtgt tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga
    16501 tcaacctgcg tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg
    16561 cgtaaaaagt ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc
    16621 atggtttcat ccacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc
    16681 gtctggttaa aatgacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg
    16741 agcggatcga gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg
    16801 ccgtggagcg ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac cagatagtta
    16861 tacagcaagc gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt
    16921 ttgatccgca ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg
    16981 aagaagtagg cgttggcgat aatcacttcc cgctgcgcct gagtgagcat tttcagataa
    17041 tggcgttcaa tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct
    17101 tctcccggct ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg
    17161 ctctgtccag gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca
    17221 aggcgtaccg cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag
    17281 taattcagcc cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc
    17341 cggcgaaaca cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac
    17401 actacgccag ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag
    17461 ccatccagca agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc
    17521 agctgtttgc cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt
    17581 gcctcgccaa tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg
    17641 atcttattgc cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat
    17701 gatcagaaag gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat
    17761 ttttgacgta gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg
    17821 ggcgtccgtg ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat
    17881 tagctttttg ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta
    17941 gctcattcac ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc
    18001 ccagatgtac gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt
    18061 agagcacgcc gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa
    18121 tgggataacg cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac
    18181 cgtaggcaaa atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc
    18241 agttttccac atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa
    18301 tatcggcgct gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa
    18361 acgcggtaaa gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt
    18421 gatcgggcat aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc
    18481 acaaaaaggt gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga
    18541 taaattcttc aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct
    18601 aagttgctgg cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat
    18661 gagtaagact ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt
    18721 gatgatgctg gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct
    18781 ggaagagctg accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc
    18841 ccgcgtactg gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc
    18901 ggcggtagtg tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg
    18961 cgtacgccgc tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca
    19021 caatatccag actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa
    19081 tagggttaat ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt
    19141 aaccggaaag gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt
    19201 gtgtcgagca gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca
    19261 ccatcgtaaa gtagaacagc gctagcgatc cggcgaaggg gatttgatac gcccagatac
    19321 caatcgccag cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc
    19381 cgatgaagat ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt
    19441 cacgttcgcg ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg
    19501 cgatcagcga cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta
    19561 ccaccagctc gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt
    19621 tgacgatctg ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga
    19681 ggatcaactg caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga
    19741 aacgcaccag tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgtgggcttt
    19801 tcagcagcag cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg
    19861 aatgctcgcc gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg
    19921 cggcgaacgg gaacaggatc acctgaatta gcacgggtaa aatcagaatc gcgcgggttt
    19981 gtggttcgcg cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca
    20041 tgctcttctc cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc
    20101 accgccgaag cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg
    20161 aggaacaggc tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg
    20221 cggatcaccg cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc
    20281 agaaaagcgg cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa
    20341 atcagcagcc ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc
    20401 agcagcgaac cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcaac
    20461 atcgccagca tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg
    20521 gtaatctccg tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc
    20581 agcgaggtga gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc cgggataata
    20641 aagtgctggc ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt
    20701 tcaaaagtct gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg
    20761 acatacccct gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc
    20821 ggtgcggtgg cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga
    20881 ccgcgaattt tccccgactg cattttggcg atcagttcct gacggttatc gctgatggtg
    20941 gcgtcgatgt agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc
    21001 tgttccagca aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa
    21061 ataaacagca gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg
    21121 atctgccgcg tctctttgac gcacagcgcc cgcacgcgat gccaggaaag gatcgggtta
    21181 ctcattgcta tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt
    21241 gggatcgggt tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg taccgctggc
    21301 gattaatttc ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa
    21361 gtgggtggtg accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa
    21421 ttcacggcgg gtgagggggt caacgccgga agtcggttcg tcgagaaaga gaatatccgg
    21481 ttcatgcatc agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc
    21541 ggtggcgtgt gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg agattttttc
    21601 gttctgcgcc cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac
    21661 cgtcaggttg ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt
    21721 accggaactc tctttcagat ccatccccag caccagcgcc tggccggaag tcggcaccag
    21781 caaaccgcac atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc
    21841 aaaaatctcc ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa
    21901 tttcttggtc agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg
    21961 caatattgca cccagcggcg attctgaggt tccggcaccg cccagcaaat caataaacgc
    22021 atcttcaaaa cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg
    22081 aatatcgtct ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat
    22141 gccatcgctg acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg
    22201 tggactggtc atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc
    22261 ctgatacagc aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc
    22321 gagatacgag gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg
    22381 ccacagttcg cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac
    22441 tttcggttcg cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag
    22501 cttgcccgcc aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa
    22561 agtttgctta cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc
    22621 catcaccgtg agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg
    22681 cagcgcgccg tcgtttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg
    22741 tttcagtaat cccgccaaca tccgcatcag tgtggtttta cctgcaccgt ccggccccac
    22801 caaccccgtc acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc
    22861 catgcccgga aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg
    22921 cctcgtcacc gaattgtacc gtcactggca ttccctggcg taacgcatca tcggcgtcgg
    22981 tcaccacaat acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt
    23041 tcggggtaaa ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg
    23101 ggcgaccatc ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt
    23161 catcaacata agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac
    23221 cttcattgag aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg
    23281 ctatcaacgt tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt
    23341 cgaggctggc tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca
    23401 atttatcctg tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt
    23461 ccaggtcatt tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt
    23521 tctgcgcata gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt
    23581 cttcatcgcg atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg
    23641 ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc
    23701 ccgctttgat agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga
    23761 aactaagatt caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc
    23821 ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc
    23881 cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag
    23941 gtcgatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt
    24001 ccagccggta cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat
    24061 caacgcatgg gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg tccaggcggc
    24121 aatcaggcgt gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta
    24181 ggctgccgtg ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc
    24241 ctgggtgagc agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggcagc
    24301 ccgatcaggc tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg
    24361 ctcgccaata aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc
    24421 gaagtagtag gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt
    24481 ggcgttcatt ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc
    24541 ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat
    24601 gattgattaa gattatgact ccatatggga gttgtccagt atggctaaga attttagcta
    24661 cgccagtcac agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc
    24721 atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa
    24781 cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc
    24841 ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc
    24901 cctgctgtgc tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca
    24961 gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg
    25021 cgtcgtccgg tacgtgcgct cattcttacc ccgacccgtg aactggcggc acagattggc
    25081 gaaaacgtcc gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggcggt
    25141 gtcagcatta acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg
    25201 ccgggacgtt tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc
    25261 ctcgtcctcg atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc
    25321 gtgttaacaa aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac
    25381 gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc
    25441 cgcaataccg cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa
    25501 cgcgaattgc tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc
    25561 cgtaccaaac acggcgctaa ccatctggct gagcagctca ataaagatgg catccgtagt
    25621 gtggcgatcc acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa
    25681 tcgggtgaca ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa
    25741 gagttgccgc acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt
    25801 atcgggcgta ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat
    25861 gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg
    25921 attccgggct atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag
    25981 caacgtggcg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc
    26041 aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg
    26101 tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa
    26161 aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt
    26221 tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct
    26281 ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag
    26341 agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt
    26401 tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg
    26461 gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt
    26521 tgtgcctgaa aatagtgttc ggaggtgggc caggtattcc cgtcaacctt gatgggcctg
    26581 gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg
    26641 tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt
    26701 ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg
    26761 ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa ccgccgcgct
    26821 taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc
    26881 ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg
    26941 gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc
    27001 cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc
    27061 gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct
    27121 taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc tgacggcgct
    27181 cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc
    27241 aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa
    27301 atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt
    27361 gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt
    27421 ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt
    27481 gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct
    27541 ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat
    27601 caccgtgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat
    27661 ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca
    27721 ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc
    27781 tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga
    27841 gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct
    27901 taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt
    27961 acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc
    28021 tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg
    28081 gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct
    28141 gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag
    28201 tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc
    28261 gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt
    28321 tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga
    28381 acaggtggag agcaaaaaat atctcggtat gttggtactg tttgccagcg gacgggcgat
    28441 gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca
    28501 gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt
    28561 gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca
    28621 ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg
    28681 ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc
    28741 gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt
    28801 ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt
    28861 accggtattt ccgatagagc aaccggaagt ccctgaaggt atagttaaaa agaaagaaaa
    28921 aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat
    28981 ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga
    29041 ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga
    29101 gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg
    29161 cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat
    29221 gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt
    29281 ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc
    29341 aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg
    29401 cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt
    29461 ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc
    29521 acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt gtctttccag
    29581 cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg
    29641 ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca
    29701 gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg
    29761 tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg
    29821 cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt
    29881 ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcatttta
    29941 atttttatct cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat
    30001 tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca
    30061 taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac
    30121 agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt
    30181 gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc
    30241 ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc
    30301 gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg
    30361 gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg
    30421 gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg
    30481 gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc
    30541 cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat
    30601 aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc
    30661 gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg
    30721 acgaccaatc cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa
    30781 cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt
    30841 aaaacgacgc atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc
    30901 actatcatca tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc
    30961 tttgccgagt gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc
    31021 gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga
    31081 agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag
    31141 gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca
    31201 agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga
    31261 aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg
    31321 cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct
    31381 tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt
    31441 ggcggaagtg atgctgtatc cactggcacc ggctgccgcc gctttctcag ccagtttcgc
    31501 ttccagggca tcgagggggg atgcgccatc ggcagaaacc acgccgattt tattcatgtt
    31561 ctgtgcctgg gatgccgtta ccggttccgc cgcgaacacg ccaaatgaca gagttgaaag
    31621 agccatagca gcaacaacag tattgatagt tttcataatt aatctctcgc aggtgatttt
    31681 tgtataagtg acgttgtttc gtcgatgtga tgagtatcac gtttttttac gagagataaa
    31741 atcgaagaga attgaccgcc ttgttcaaat aaattgattg atatctaaca tactgataaa
    31801 ccatttattt gttgtactta tggacttgtt ttatgccgcg tggcagtcac agcagcgtta
    31861 aaggcacgga atgccacgca aaatacaaat tatactcgct atgcatctcg ccagttgtag
    31921 ttaacgtaac tgtctgtctt tcgaacctct gcgattatat cctgtatacg ctggtttttg
    31981 taaatctttc tcctgctgac aatgaataca taagcgcacg ccaggaatgg cttcccgacg
    32041 ggcttgcggg atgggggcac cgcactcttc acattcatac aggctttcgc cacgcggaat
    32101 ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg ttgatctgtt cgttgacggc
    32161 gtcatcgtta gcccaagcgg atgccatatc gtcctcccca tatcaatgct tgtacagtta
    32221 agtgtagcta atatagggac gaacctgggc agttcaagcg tcagatctcc gaccattccc
    32281 gcagcagatt atgataaaga ttgagcagcg acaggatctc ttcattttca ccatagcggc
    32341 ttttcagcga ctgaatattt ttgtccagtt caaacagcat ggcgcgcttt ttatcatcgc
    32401 ggatcatcga ctggatccac ataaatgatg ccactcgtac gccgcgggtt acgggtgtca
    32461 cgcaatgcag gctgctggag ggatacaaca ccagatcacc tgccgggagt tttacccgat
    32521 gttgtccaaa ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt ggatcgctta
    32581 aaaacagcgt ggcagaaagg tcagtgcgca tccagccgtt ttgtggatgg ctgcgtactg
    32641 cgccatccac atggaaacca taggtttcat tgttctgata gcgattaaac agcggcgtgg
    32701 aaagggtacg cggcaaggca gcggcaaaga acaatgcatg ttggttaacc gcgttcagca
    32761 cctcattttg cagtgcggcg tataacgcgc tgcgggtgtc tacttgctga ttgttcttaa
    32821 cttgtgcacc ttgtgctccg gtggtgacgc gtccatccac ccattcggct tgttccagtt
    32881 gttcgcgaaa atgggcgacg tcctgtggcg ataacacgcc gggaatgtgg tacatcatac
    32941 aagttctcca aaaagtgggg cctgcgcccc acatctgaat cagaaatgca tattggctgt
    33001 gagcaagaag gttcttggct cgcccgggtg ataacggtag ccgctcttgt tgattgaggc
    33061 gacgtaatcg gtatcaaaca ggttgtaaac gtttagctgg aagtcgagat tgcgattaac
    33121 ccgatacccc agtttggcat cggcgaccca gtaaccttct gtaaatgctg gcgttcccac
    33181 cgcgccgtct gaacctttat gtatactgcc gatatagcgt gcgcccgcgc caacagagat
    33241 atcatcggtt gcctgatatt ggctccataa ggtgaaggcg tgctccgggg tatacggcag
    33301 cgatgaggaa ccatcctggg caacatcttt gccgtttttg atggttgctt tttgctgggt
    33361 atagccgcca atcacctgcc acgcgggagt gatattccct gccacggata tctcatagcc
    33421 ttcgacgcgt ttcttaccgt attgcgagta agtcccgtca tcattttgct caacttcatt
    33481 ttcgatatca gtacggaaca gcgcggcggt gagcaacagg cgtttatcca gaacctgcca
    33541 tttggtgcca atctcgctgg tgttggcttt ttgcggttta aaatcggtgc ggttggcact
    33601 gttaccactg ccagactgcg caagggcgaa gttgttgccg cccggaggct gctgggaaac
    33661 ggcatagtta atatagacat tgccgttttc cgtcaggtgg tacagcgccc cggctttcca
    33721 gttcaccaga ttgcccgact tggcggtgtc gacggtggtg actggagaac cttttgccac
    33781 accagctggg caggtgatgg caccgcgtcc gctgccgccg caggcggtgg cactgtcata
    33841 ttcagtatga taattatcca gacggatccc gccgttcagc tcaaaatcac gggtgatttg
    33901 cagcgtatcg aaggcgtaaa ttgcgaaggt atccgtctga ccattggcgt ttgcgccgtt
    33961 gcgcgtcagg ccgccgggat gaattctgct gtcaggatga taaatattta ccgccggtaa
    34021 cgtcaccgga ttaacgccat agttagtctg cgtttcacgg gtaaattcca cgccggtact
    34081 gacatcatga ccgatagaac cggtatagaa cgtcgaggtc aggttggtct ggttggtgag
    34141 aattttatta ctcacatctt tggtattcgc cgtgcgtgac taggtccagc tattcacatc
    34201 gctggtgggc tgagtaatat tcgacgcccc gcccataatc gccgtcatca ggtaatcctg
    34261 ctttacgcgc gaccaacggg tagtattgcg aatggtggtg ttatcgttga tgtcgtgctc
    34321 aaaacgcatg gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg tgccgtaaaa
    34381 gttatgagta tcaacttttc cggaatgatt cagggccgcc gctcccgcag atggggcaga
    34441 atagcccggc aaaccgatgg tcggaatgcc gccgtctggc gtgttgtgct gagtgacatg
    34501 cagataatta agatacaaac gatttgctgt accaaggcca aaagcgacag aaggggcgac
    34561 gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg tgggtttttt cgcccatcac
    34621 attcaggcgc accgcggtag tatcaccaat gacctgattg acgtccagcg tgccgcggcg
    34681 gaaccaggcg ctgccaatac tggcggaggc gtcaatgccg gaatcattgc gcggctgctt
    34741 gctgatcata ttgatcgagc ctgtcggtgc gctgcgcccg tagtcggtgc cggacggccc
    34801 tttaatcact tcgacctgct cggtattgaa ggtgtcgcgc gagacgctgc cgatatcgcg
    34861 aataccatca atataaatac tgttagaggt atcggcacca cgcatataaa tggcgtcgcc
    34921 agtggtggag ttaccgttct cacccgcaaa aaacgcaccc acgccgg
//
LOCUS       DAZPEU010000024        34628 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPEU010000024 DAZPEU010000000
VERSION     DAZPEU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.024"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1101)
                     /locus_tag="ACHU2L_001063"
     CDS             complement(<1..1101)
                     /locus_tag="ACHU2L_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1356010.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHU2L_001064"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHU2L_001064"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR1356011.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHU2L_001065"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHU2L_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1356012.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHU2L_001066"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHU2L_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1356013.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHU2L_001067"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHU2L_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1356014.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHU2L_001068"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHU2L_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1356015.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="ACHU2L_001069"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="ACHU2L_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1356016.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHU2L_001070"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHU2L_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR1356017.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHU2L_001071"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHU2L_001071"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR1356018.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHU2L_001072"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHU2L_001072"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR1356019.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHU2L_001073"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHU2L_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR1356020.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHU2L_001074"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHU2L_001074"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR1356021.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="ACHU2L_001075"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="ACHU2L_001075"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR1356022.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="ACHU2L_001076"
     CDS             11851..12015
                     /locus_tag="ACHU2L_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356023.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="ACHU2L_001077"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="ACHU2L_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR1356024.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="ACHU2L_001078"
     CDS             13524..13769
                     /locus_tag="ACHU2L_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR1356025.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="ACHU2L_001079"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="ACHU2L_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR1356026.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="ACHU2L_001080"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="ACHU2L_001080"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR1356027.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="ACHU2L_001081"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="ACHU2L_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR1356028.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="ACHU2L_001082"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="ACHU2L_001082"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR1356029.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="ACHU2L_001083"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="ACHU2L_001083"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR1356030.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="ACHU2L_001084"
     CDS             19432..19785
                     /locus_tag="ACHU2L_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR1356031.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="ACHU2L_001085"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="ACHU2L_001085"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR1356032.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="ACHU2L_001086"
     CDS             21085..22212
                     /locus_tag="ACHU2L_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR1356033.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="ACHU2L_001087"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="ACHU2L_001087"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR1356034.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="ACHU2L_001088"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="ACHU2L_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHU2L_001089"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHU2L_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHU2L_001090"
     CDS             complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHU2L_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR1356035.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHU2L_001091"
     CDS             26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHU2L_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR1356036.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="ACHU2L_001092"
     CDS             26834..27583
                     /locus_tag="ACHU2L_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR1356037.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="ACHU2L_001093"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="ACHU2L_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR1356038.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHU2L_001094"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHU2L_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR1356039.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHU2L_001095"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHU2L_001095"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR1356040.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHU2L_001096"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHU2L_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR1356041.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHU2L_001097"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHU2L_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR1356042.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHU2L_001098"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHU2L_001098"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR1356043.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="ACHU2L_001099"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="ACHU2L_001099"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR1356044.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..>34628)
                     /gene="acnA"
                     /locus_tag="ACHU2L_001100"
     CDS             complement(32673..>34628)
                     /gene="acnA"
                     /locus_tag="ACHU2L_001100"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR1356045.1"
                     /translation="PVSMFIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKF
                     VEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEK
                     YAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELK
                     VNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLG
                     LKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIE
                     TAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIG
                     HDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYG
                     WQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAG
                     RYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVV
                     SIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNL
                     IGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCR
                     CRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT         8163 a         9025 c         8744 g         8696 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataaacatg
    34621 gaaaccgg
//
LOCUS       DAZPEU010000025        31853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPEU010000025 DAZPEU010000000
VERSION     DAZPEU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.025"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..860)
                     /gene="rarD"
                     /locus_tag="ACHU2L_001101"
     CDS             complement(<1..860)
                     /gene="rarD"
                     /locus_tag="ACHU2L_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR1356046.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDA"
     gene            complement(912..1379)
                     /gene="yigI"
                     /locus_tag="ACHU2L_001102"
     CDS             complement(912..1379)
                     /gene="yigI"
                     /locus_tag="ACHU2L_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR1356047.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1544..2413
                     /gene="pldA"
                     /locus_tag="ACHU2L_001103"
     CDS             1544..2413
                     /gene="pldA"
                     /locus_tag="ACHU2L_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR1356048.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2546..4375
                     /gene="recQ"
                     /locus_tag="ACHU2L_001104"
     CDS             2546..4375
                     /gene="recQ"
                     /locus_tag="ACHU2L_001104"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR1356049.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4439..5059
                     /gene="rhtC"
                     /locus_tag="ACHU2L_001105"
     CDS             4439..5059
                     /gene="rhtC"
                     /locus_tag="ACHU2L_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR1356050.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5121..5741)
                     /gene="rhtB"
                     /locus_tag="ACHU2L_001106"
     CDS             complement(5121..5741)
                     /gene="rhtB"
                     /locus_tag="ACHU2L_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR1356051.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5852..6874
                     /gene="pldB"
                     /locus_tag="ACHU2L_001107"
     CDS             5852..6874
                     /gene="pldB"
                     /locus_tag="ACHU2L_001107"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR1356052.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6882..7682
                     /gene="yigL"
                     /locus_tag="ACHU2L_001108"
     CDS             6882..7682
                     /gene="yigL"
                     /locus_tag="ACHU2L_001108"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR1356053.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7758..8657
                     /gene="bioP"
                     /locus_tag="ACHU2L_001109"
     CDS             7758..8657
                     /gene="bioP"
                     /locus_tag="ACHU2L_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR1356054.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8545..9498)
                     /gene="metR"
                     /locus_tag="ACHU2L_001110"
     CDS             complement(8545..9498)
                     /gene="metR"
                     /locus_tag="ACHU2L_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR1356055.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9734..11995
                     /gene="metE"
                     /locus_tag="ACHU2L_001111"
     CDS             9734..11995
                     /gene="metE"
                     /locus_tag="ACHU2L_001111"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR1356056.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12034..12849)
                     /locus_tag="ACHU2L_001112"
     CDS             complement(12034..12849)
                     /locus_tag="ACHU2L_001112"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR1356057.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13111..13872
                     /gene="udp"
                     /locus_tag="ACHU2L_001113"
     CDS             13111..13872
                     /gene="udp"
                     /locus_tag="ACHU2L_001113"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR1356058.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14013..15440
                     /gene="rmuC"
                     /locus_tag="ACHU2L_001114"
     CDS             14013..15440
                     /gene="rmuC"
                     /locus_tag="ACHU2L_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR1356059.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15535..16290
                     /gene="ubiE"
                     /locus_tag="ACHU2L_001115"
     CDS             15535..16290
                     /gene="ubiE"
                     /locus_tag="ACHU2L_001115"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR1356060.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16304..16909
                     /gene="ubiJ"
                     /locus_tag="ACHU2L_001116"
     CDS             16304..16909
                     /gene="ubiJ"
                     /locus_tag="ACHU2L_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR1356061.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16906..18546
                     /gene="ubiB"
                     /locus_tag="ACHU2L_001117"
     CDS             16906..18546
                     /gene="ubiB"
                     /locus_tag="ACHU2L_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR1356062.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18625..18894
                     /gene="tatA"
                     /locus_tag="ACHU2L_001118"
     CDS             18625..18894
                     /gene="tatA"
                     /locus_tag="ACHU2L_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR1356063.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18898..19413
                     /gene="tatB"
                     /locus_tag="ACHU2L_001119"
     CDS             18898..19413
                     /gene="tatB"
                     /locus_tag="ACHU2L_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR1356064.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19416..20192
                     /gene="tatC"
                     /locus_tag="ACHU2L_001120"
     CDS             19416..20192
                     /gene="tatC"
                     /locus_tag="ACHU2L_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR1356065.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20234..21016
                     /gene="tatD"
                     /locus_tag="ACHU2L_001121"
     CDS             20234..21016
                     /gene="tatD"
                     /locus_tag="ACHU2L_001121"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR1356066.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21013..21501)
                     /gene="rfaH"
                     /locus_tag="ACHU2L_001122"
     CDS             complement(21013..21501)
                     /gene="rfaH"
                     /locus_tag="ACHU2L_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR1356067.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21668..23161
                     /gene="ubiD"
                     /locus_tag="ACHU2L_001123"
     CDS             21668..23161
                     /gene="ubiD"
                     /locus_tag="ACHU2L_001123"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR1356068.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23207..23908
                     /gene="fre"
                     /locus_tag="ACHU2L_001124"
     CDS             23207..23908
                     /gene="fre"
                     /locus_tag="ACHU2L_001124"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR1356069.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24100..25263)
                     /gene="fadA"
                     /locus_tag="ACHU2L_001125"
     CDS             complement(24100..25263)
                     /gene="fadA"
                     /locus_tag="ACHU2L_001125"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR1356070.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25273..27462)
                     /gene="fadB"
                     /locus_tag="ACHU2L_001126"
     CDS             complement(25273..27462)
                     /gene="fadB"
                     /locus_tag="ACHU2L_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR1356071.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27652..28983
                     /gene="pepQ"
                     /locus_tag="ACHU2L_001127"
     CDS             27652..28983
                     /gene="pepQ"
                     /locus_tag="ACHU2L_001127"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR1356072.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28983..29597
                     /locus_tag="ACHU2L_001128"
     CDS             28983..29597
                     /locus_tag="ACHU2L_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR1356073.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29636..31087
                     /gene="trkH"
                     /locus_tag="ACHU2L_001129"
     CDS             29636..31087
                     /gene="trkH"
                     /locus_tag="ACHU2L_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR1356074.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31099..31644
                     /gene="hemG"
                     /locus_tag="ACHU2L_001130"
     CDS             31099..31644
                     /gene="hemG"
                     /locus_tag="ACHU2L_001130"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR1356075.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7397 a         8115 c         8720 g         7621 t
ORIGIN      
        1 gcgtccatca caaaaattgc cagcgccacc caaataaagg tgaaagtcac catcttatcg
       61 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
      121 tactgaaaaa agcctaacgt tgagagacgc aggcgcgtgg cagcggcagt aaaacacaac
      181 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
      241 tgccccatat ggctggttga actgtcggca atagcaaaca ggtaaatcgc cgccacgggc
      301 agcagccaca tggtttcaat taacatgccg gtttgtgctt ctaccgcaat cttcttgcgc
      361 accaggccgt agaaggcaaa actaaatgcc agccccagcg cgataatagg tagcgaacca
      421 aaagtccaca gctggaccaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
      481 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
      541 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
      601 cagttgccac caatcagtac ggcagagact gccagcataa aaattttctg tggcgtctga
      661 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
      721 aacgaccaga tcacgcgatg cgtcaggatc tcatcggcgg gcacgtagta aatcagcttg
      781 aagtatgctg gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
      841 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
      901 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
      961 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     1021 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     1081 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     1141 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gactccgccg
     1201 tgcaaaatgc tttgcgccca gttacccacc atcatcggct gatttttaaa ggccagctgt
     1261 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     1321 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     1381 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     1441 ctactttagg aagggataac taacggcaat ggagttaagt atgtaaaata gcccgcagaa
     1501 aaacattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     1561 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     1621 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     1681 tgacaatccg ttcacgctct atccttatga caccaactac ctcatctaca cccaaaccag
     1741 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     1801 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccaaactc
     1861 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     1921 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     1981 ccgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     2041 gcgttcagac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     2101 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     2161 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     2221 ggtgctcagt gcgaaaggac agtacaactg gaacaccggc tacggcggcg cggagttagg
     2281 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     2341 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtagggg ttatgctaaa
     2401 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaatccagca tccaggatta
     2461 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     2521 tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     2581 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     2641 aattatcgac actgtgcttt ccggtcgcga ttgcctggtt gttatgccca ctggtggcgg
     2701 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     2761 accgctgatt tcgttgatga aagatcaggt ggatcaacta caggccaacg gcgtggcggc
     2821 ggcgtgcctt aactcgacgc aaacccgcga acagcaactt gaagtgatga caggctgccg
     2881 caccgggcaa attcgcttac tgtatatcgc cccggaacgc ctgatgctgg ataactttct
     2941 tgagcatctg gcgcactgga atccggtgtt attagccgtc gatgaagcgc actgtatctc
     3001 ccaatggggt cacgatttcc gcccggaata tgccgcgctc ggtcagttgc gccagcggtt
     3061 cccgacgctg ccgtttatgg cgctgaccgc cacagccgac gacaccacgc gccaggatat
     3121 cgtgcgcctg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     3181 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     3241 ggaacagcgc ggtaagtctg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     3301 cgctgcgcgc ctgcaaagca ggggtattag cgcggcggcc tatcatgccg ggctggaaaa
     3361 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gatctgcaaa ttgtggtggc
     3421 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     3481 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     3541 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     3601 ggaagagaag ccgcaggggc agttgcagga tatcgagcgc cacaaactca atgcgatggg
     3661 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     3721 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     3781 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     3841 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     3901 tgacaaactg aaagtctatg gtatgggccg tgataaaagc catgaacatt gggtgagcgt
     3961 gatccgccag ctgattcatc tcggcctggt gacgcaaaat attgcccagc attctgccct
     4021 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     4081 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     4141 tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     4201 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     4261 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     4321 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     4381 agcataaaaa agtgccagta tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     4441 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     4501 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     4561 cgtgctgggc attacctgcg gcgtaatggt ttgggctggg attgcgttgc ttggcctgca
     4621 tttgattatc gaaaaaatgg cctggctgca tacgctgatt atggtaggcg gtggcctgta
     4681 tctctgctgg atgggttacc agatgctacg cggtgcactg aaaaaagagg ctgtatctgc
     4741 gcctgcacca caggttgagc tggcgaaaag cgggcgcagt ttcctgaaag gcttactgac
     4801 caatctcgcc aatccgaaag cgattatcta ttttggttcg gtattctcat tgtttgtcgg
     4861 cgataacgtt ggcactaccg agcgctgggg cattttcgcg ttgattattg tcgaaacact
     4921 ggcgtggttt accgtcgttg ccagtctgtt tgccctgccg caaatgcgcc gtggttatca
     4981 acgtctggcg aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca
     5041 tttgattatt tcgcggtgat gccagacgcg tcttcagagt aagtcggata aggcgtttac
     5101 gccgcatccg acattatttt tcacgcatgc ctcgccgatg ctaacagcgc tcccaccagc
     5161 ataaacaacg agccgaaaat cttattcagc gccttcatct gctttggtcc tttaatccat
     5221 agagcaatcc tttgagcaag ggtggcgtaa ccgatcatca caataatatc gaccacaata
     5281 gtggtgacgc cgagcacgat atactgcatc agttgcggct gttgcggcat gatgaattgc
     5341 ggaaatagcg ccgccagaaa cacaatactt ttgggattgg tgagattcac aaaaactgcg
     5401 cgctggaaca aatgtcgacg cgattgagta gaggccagcg atttaagatc aattgcgcca
     5461 gcggcgcgcc actgctggat tcccagccaa atcaagtaag ccgcgcctgc ccactttaac
     5521 acttcaaacg caatcactga gcgggaaaat agcgtcccca accccacgcc aaccagcaca
     5581 atatgaatcg ccagtccggt ctgaagccca gcaatagacg ccaccgcgcc gcgataaccg
     5641 tggttgagcg aggtggtcat agtgttgatt gcaccagagc ctggcgacag gcttaaaatg
     5701 atcgatgtca gcaggtaggc aaaccaccat tctaaggtca tgatgaactc ccggtgtgtc
     5761 tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt caggcacgct taaaaaaacg
     5821 attttacgtg gtttaagagg cagattaccc gatgtttcag cagcaaaaag actgggaaac
     5881 aagagaaaac gcgtttgctg cttttaccat gggaccgctg actgatttct ggcgtcagcg
     5941 tgatgaagca gagtttactg gtgtggatga cattccggtg cgctttgtcc gttttcgcgc
     6001 acagcaccat gaccgggtgg tagtcatctg cccggggcgt attgagagct acgtaaaata
     6061 tgcggaactg gcctacgacc tgttccattt ggggtttgat gtcttaatca tcgaccatcg
     6121 cgggcaggga cgttccggtc gcctgttagc cgatccgcat ctcgggcatg ttaatcgctt
     6181 taatgattat gttgatgatc tggcggcatt ctggcagcag gaggttcagc ccggtccgtg
     6241 gcgtaaacgc tatatactgg cacattcgat gggcggtgcg atctccacat tatttctgca
     6301 acgccatcca ggtgtatgtg acgccattgc gctaactgcg ccaatgtttg ggatcgtgat
     6361 tcgtatgccg tcatttatgg cacggcagat cctcaactgg gccgaagcgc atccacgttt
     6421 ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg ttgccgtttg ctatcaacgt
     6481 actgacccac agcagacagc gatatcgacg taacttacgc ttctatgctg atgacccaac
     6541 gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa agtattctgg ctggcgaaca
     6601 ggtgttagcc ggtgcgggtg atgacgccac gcaaacgctt ctcttacaag ctgaagagga
     6661 acgcgtggtg gataaccgca tgcacgaccg tttttgtgaa ctccgcaccg ccgcgggcca
     6721 tcctgtcgaa ggaggacggc cgttggtaat aaaaggtgct taccatgaga tcctttttga
     6781 aaaggacgca atgcgctcag tcgcgctcca cgccatcgtt gattttttca acaggcataa
     6841 ctcacccagc ggaaaccgct ctacagaggt ttaaatttct tatgtaccag gttgttgcgt
     6901 ctgatttaga tggcacgtta ctttctcccg accatacgtt atccccttac gccaaagaaa
     6961 ctctgaagct gctcaccgcg cgcggcatca actttgtgtt tgcgaccggt cgtcaccacg
     7021 ttgatgtggg gcaaattcgc gataatctgg agattaagtc ttacatgatt acctccaatg
     7081 gtgcgcgcgt tcacgatctg gatggcaatc tgatttttgc tcataacctg gatcgcgaca
     7141 ttgccagcga tctgtttggc gtagtcaacg acaatccgga catcattact aacgtttatc
     7201 gcgacgacga atggtttatg aatcgccatc gcccggaaga gatgcgcttt tttaaagaag
     7261 cggtgttcaa atatgcgctg tatgagccgg gattactgga gccggaaggc atcagcaaag
     7321 tgtttttcac ctgcgattcc catgaacaac tgctgccgct ggagcaggcg attaacgctc
     7381 gctggggcga tcgcgtcaac gtcagtttct ctaccttaac ctgtctggaa gtgatggcgg
     7441 gcggcgtgtc gaaaggccat gcgctggaag cagtggcgaa gaaactgggc tacagcctga
     7501 aggattgtat tgcgtttggt gacgggatga acgacgccga aatgctgtcg atggcgggga
     7561 aaggctgcat tatgggcagt gcgcaccagc gtctgaaaga tctgcatccg gagctggaag
     7621 ttattggtac taatgccgac gacgcggtgc cgcattatct gcgtaaactc tatttatcgt
     7681 aatcgttctt tatttggtca gttgtcaacc tgatacttcg ctacaatgga tactcgttaa
     7741 tcaaagagtt ttccattgtg gcgttactta tcatcaccac gattctgtgg gccttctcct
     7801 ttagctttta tggcgagtac cttgcggggc acgtcgatag ctattttgcg gtgctggtgc
     7861 gcgttggctt ggcggcactc gtttttctgc cgtttctgcg tacccgtagc aatagcctga
     7921 aaacggtcgg cctgtatatg ctggtgggcg cgatgcagct cggcgtgatg tatatgctga
     7981 gtttccgcgc ttatctctac ctgacggtct ccgaattgtt gctgttcacc gtactgacgc
     8041 cgctctacat cacgctgatt tatgacatca tgagtaagcg ccgtctgcgc tggggctatg
     8101 cttttagcgc cttgctggcg gtgattggtg ccgggattat tcgctatgat caggtcaccg
     8161 accatttctg gactggcttg ttgctggtgc aactctccaa tatcactttt gccatcggca
     8221 tggtgggtta caaacgcctg atggaaactc gcccgatgcc gcagtataac gcctttgcgt
     8281 ggttctatct tggcgcgttc ctggtggcgg taattgcatg gttcttgctg ggaaatgcgc
     8341 agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt gtttcttggc gtggtggctt
     8401 ccgggattgg ctactttatg tggaactacg gcgcgacgca ggtggacgcc ggaacgctgg
     8461 gcattatgaa taatatgcac gttccggcag ggctgctggt aaacctggct atctggcacc
     8521 aacagccgca ctggccaacg tttattacag gcgcgctggt gatcctggcc tcactgtggg
     8581 tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc agatgatcgc aggcgtgatt
     8641 gcgcgctgag cgaataaacg cttccgtaac tggctgacgc tgctcgccat cgcgcacggc
     8701 ggcgtacagt cggctccaca agccttcgcc cagggttttg gtcaccacca gaccctggcg
     8761 ctcaaaactc tctactaccc aatgcggtag cgcggcaata cccatccgcg cggcaaccat
     8821 ttgaatcaac aataaggtgt tatcgacgct tttcagtgac gggctgacgc ctgccggctg
     8881 aagaaaatgc cgccagacat ccagtcgact acgctgcacc ggataaatta atagcgtctc
     8941 gctggcgaga tcttccggtg taatacgcgt ttttgccgcc agtggatggt caggtgctaa
     9001 caccaggcgt acttcataat cgaacatcgg cgaataatgc aggccactgc gcggcaggat
     9061 atcggaggtc atcaccagat ccagctcgcc ctgttgcaac gcgggctgcg ggtcaaacgt
     9121 cacgcccgat ttaaaatcca tctctacctg cggccagttc ttatggaaat ttcctaaagc
     9181 gggtgtcagc cactgaatac agctatggca ctcaatggca atgcgcagac gcgtctgctg
     9241 cggttcattg caggcttgca gtgcctggct gatttgcggc agcacctggt ttgccagttg
     9301 caacaggatt tctccctgcg gtgtaaagcg tagcggctgg ctcttacgca caaatagccg
     9361 gaagccaagg cgttgttcca gatcgctaaa ctggtgagac agggcggatt gcgtctgatg
     9421 caacgttgcc gcagcggctg cgagcgagcc gcagttccgc aacgcttgta gcgttttcag
     9481 gtgttttact tcgatcatga aagtccttca cttcggcatg aataatttgc gcttgaggaa
     9541 tatacagtaa ccgccaatta tggatgtgta aacatctgga cggctaaaat cattcgtctt
     9601 ttaaatttat ggtgcgttgg ctgcgtttct ccaccccggt cacttacttc agtaagctcc
     9661 cggcgatgaa taaacttgcc gccttcccta aattcaaaat ccataggatt tacatataat
     9721 tagaggaaga aaaatgacaa tattgaatca caccctcggt ttccctcgcg ttggcctgcg
     9781 tcgcgagctg aaaaaagcgc aagaaagtta ttgggcgggg aactccacgc gtgaagaact
     9841 gctgacagta gggcgtgaac tgcgtgctcg tcactgggat caacaaaagc aagcgggtat
     9901 cgacctgctg ccggtgggcg attttgcctg gtacgatcat gtactgacca ccagtttgct
     9961 gctgggtaat gttccgccac gtcatcagaa caaagacggt tcggtagata tcgacaccct
    10021 gttccgtatt ggtcgtggac gtgcgccgac tggcgaacct gcggcggcag cggaaatgac
    10081 caaatggttt aacaccaact atcactacat ggtgccggag ttcgttaaag gccaacagtt
    10141 caaactgact tggacgcagc tgctggagga agtggacgag gcgctggcgc tgagccacaa
    10201 cgttaagcct gtgctgctgg ggccggttac ttacctgtgg ctggggaaag tgaaaggtga
    10261 acagtttgac cgccttagcc tgctgaacga cattctgccg gtttatcagc aagtgctggc
    10321 agaactggcg aaacgcggca tcgagtgggt acagattgat gaacccgcgc tggtactgga
    10381 actaccacag gcgtggctgg acgcatacaa acccgcttac gacgcgctcc agggacaggt
    10441 gaaactgctg ctgaccacct attttgaagg cgtaacgcca aatctcgaca cgattactgc
    10501 gctgcctgtt caggggctgc atgtggacct tgtacatggt aaagataacg ttgttgaact
    10561 gcacaagcgc ctgccttctg actggctgct gtctgcgggt ctgatcaatg gtcgtaacgt
    10621 ctggcgcgcc gatcttactg agaaatatgc gcaaattaag gacattgtcg gcaaacgtga
    10681 tttgtgggtg gcatcttcct gctcgttgct gcacagcccc atcgacctga gcgtggaaac
    10741 gcgtcttgat gcagaagtga aaagctggtt tgccttcgcc ctacaaaaat gccatgaact
    10801 ggcactgctg cgcgatgcgc tgaacagtgg tgacacggca gctctggcag agtggagcgc
    10861 cccaattcag gcgcgtcgtc actctactcg tgtacataat ccggcggtag aaaagcgtct
    10921 ggcggcgatc accgcccagg acagccagcg tgcgaatgtc tatgaagtgc gtgctgaagc
    10981 ccagcgtgcg cgttttaaac tgccagcgtg gccgaccacc acgattggtt ccttcccgca
    11041 aaccacggaa attcgtaccc tgcgtctgga tttcaaaaag ggcaatctcg acgccaacaa
    11101 ctaccgcacg ggcattgcgg aacatatcaa gcaggccatt gttgagcagg aacgtttggg
    11161 actggatgtg ctggtacatg gcgaggccga gcgtaatgac atggtggaat actttggcga
    11221 gcacctcgac ggatttgtct ttacgcaaaa cggttgggta cagagctacg gttcccgctg
    11281 cgtgaagcca ccgattgtca ttggtgacat tagccgcccg gcaccgatta ccgtggagtg
    11341 ggcgaagtat gcgcaatcgc tgaccgacaa accggtgaaa gggatgctga cggggccggt
    11401 gaccatactc tgctggtcgt tcccgcgtga agatgtcagc cgtgaaacca tcgccaaaca
    11461 gattgcgctg gcgctgcgtg atgaagtggc cgatctggaa gccgctggaa ttggcatcat
    11521 ccagattgac gaaccggcgc tgcgcgaagg tttaccgctg cgtcgtagcg actgggatgc
    11581 gtatctccag tggggcgtag aggccttccg tatcaacgcc gccgtggcga aagatgacac
    11641 acaaatccac actcacatgt gttattgcga gttcaacgac atcatggatt cgattgcggc
    11701 gctggacgca gacgtcatca ccatcgaaac ctcgcgttcc gacatggagt tgctggagtc
    11761 gtttgaagag tttgattatc caaatgaaat cggtcctggc gtctatgaca ttcactcgcc
    11821 aaacgtaccg agcgtggaat ggattgaagc cttgctgaag aaagcggcaa aacgcattcc
    11881 ggcagagcgc ctgtgggtca acccggactg tggcctgaaa acgcgcggct ggccagaaac
    11941 ccgcgcggca ctggcgaaca tggtgcaggc ggcgcagaat ttgcgtcggg ggtaaatcca
    12001 aaccgggtgg taataccacc cggtcttttc tcattacagc gacttcttcc caccatactg
    12061 cttaaaccat tccagcatac gctgccagcc atctttcgca gattcggcat gatagctcgg
    12121 gcgataatca gcgttgaatg catgcccggc atccgggtac acgataatct ctgctttcgc
    12181 attagcagcc cgcagcgcct ggcgcatcgt ttcaacgctc tcctgcggaa tgctgttatc
    12241 ctgaccacca tataagccga gaaccggcgc gttaagatcg gttgcgatat caacaggttg
    12301 tttcggtgaa ttcagcgact tgtcgcccgt cagtttgccg taccacgcca ctgcggcttt
    12361 tagctgtgga ttatgcgcgg catacagcca ggtgatacgt ccaccccagc agaatccggt
    12421 gatcattaaa cgatgaacat cgccgccgtt gcgggaagcc caactggcga catgatcgag
    12481 atcggccagc acctgcgagt caggcacttt tgctaccaga ccgctaagca acgtggggat
    12541 atcggcaaaa tcattcggat cgccttcgcg gaagtaaagt tcaggtgcga tagccagata
    12601 cccctccagc gccagacggc gacaaatgtc gcggatatgt tcatgcacgc caaaaatttc
    12661 ctgcactaca atgaccactg gcagtgggcc atcgctttgc tttggtctgg catggtaagc
    12721 aggcatgtta tccccttgtg aagggataga ggtgaagccc gccacaatcg cgtcgtccgg
    12781 ggtctgaacg atggtcgaag cgagaggcga tgcagcaggt gcaaatccag attgttgtgt
    12841 tgttgccatg gtattctccg tacccttata aaaatgttgc gcaatgttaa ctatagtcag
    12901 catgcaacaa atcacattgc ctgaatcggc tcatctttta tgcagtcctg cagaatgaag
    12961 agtgatttat gtgatatgaa tcacttttgg tgggtaaatt tatgcaacgc atttgcgtca
    13021 tggtgatgag tatcacgaaa aaatgttaaa cccttcggta aagtgtcttt ttgcttcttc
    13081 tgactaaacc gattcacaga ggagttgtat atgtccaagt ctgatgtttt tcatctcggc
    13141 ctcactaaaa acgatttaca aggggctacg cttgccatcg tccctggcga cccggatcgt
    13201 gtggaaaaga tcgccgcgct gatggataag ccggttaagc tggcatctca ccgcgaattc
    13261 actacctggc gtgcagagct ggatggtaaa cctgttatcg tctgctctac cggtatcggc
    13321 ggcccgtcta cctctattgc tgttgaagag ctggcacagc tgggcattcg caccttcctg
    13381 cgtatcggta caacgggcgc tattcagccg catattaatg tgggtgatgt cctggttacc
    13441 acggcgtctg tccgtctgga tggcgcgagc ctgcacttcg caccgctgga attcccggct
    13501 gtcgctgatt tcgaatgtac gactgcgctg gttgaagctg cgaaatctat tggcgcgaca
    13561 actcacgttg gcgtgacagt ttcttctgat accttctacc caggtcagga acgttacgat
    13621 acttactctg gtcgcgtagt tcgtcacttt aaaggttcta tggaagagtg gcaggcgatg
    13681 ggcgtaatga actatgaaat ggaatctgca actctgctga ccatgtgtgc aagtcagggc
    13741 ctgcgtgccg gtatggtagc gggtgttatc gttaaccgca cccagcaaga gatcccgaat
    13801 gctgagacga tgaaacaaac cgaaagccat gcggtgaaaa tcgtggtgga agcggcgcgt
    13861 cgtctgctgt aattctcttc tcctgtctga aggccgacgc gttcggcctt ttgtattttt
    13921 gcgtagcgcc tcgcaggaaa tgcctttcca actggacgtt tgtacagcac aattctattt
    13981 tgtgcgggta agttgttgcg tcaggaggcg ttgtggattt ctcaatcatg gtttacgcag
    14041 ttattgcgtt ggtgggtgtg gcaattggct ggctgtttgc cagttatcaa catgcgcagc
    14101 aaaaagccga gcaattagct gaacgtgaag agatggtcgc ggagttaagc gcggcaaaac
    14161 aacaaattac ccaaagcgag cactggcgtg cagagtgtga attgctcaat aacgaagtgc
    14221 gcagcctgca aagtattaac acctctctgg aggccgatct gcgtgaagta accacgcgga
    14281 tggaagccgc acagcaacat gctgacgata aaattcgcca gatgattaac agcgagcagc
    14341 gcctcagtga gcagtttgaa aacctcgcca accgtatttt tgagcacagc aatcgccggg
    14401 ttgatgagca aaaccgtcag agtctgaaca gcctgttgtc gccgctacgt gaacaactgg
    14461 acggtttccg ccgtcaggtt caggacagct tcggtaaaga agcacaagaa cgccataccc
    14521 tgacccacga aattcgcaat ctccagcaac tcaacgcgca aatggcccag gaagcgatca
    14581 acctgacgcg cgcgctgaaa ggcgacaata aaacccaggg caactggggc gaggtagtat
    14641 tgacgcgggt gctggaggct tccggtctgc gtgaagggta tgaatatgaa acccaggtca
    14701 gcatcgaaaa tgacgcccgc tcgcggatgc agccggatgt catcgtgcgg ctgccgcagg
    14761 gaaaagatgt ggtgatcgac gccaaaatga cgctggtcgc ctatgaacgc tattttaacg
    14821 ccgaagacga ctacacccgc gaaagcgcgc tacaggaaca tatcgcgtcg gtgcgtaacc
    14881 atatccgttt gctgggacgc aaagattatc aacagctgcc ggggctgcga actctggatt
    14941 acgtgctgat gtttattccc gttgaacccg cttttttact ggcgcttgac cgccagccgg
    15001 agctgatcac cgaagcgttg aaaaacaata tcatgctggt tagcccgact acgctgctgg
    15061 tggcgttgcg tactattgcc aacctgtggc gttatgagca tcaaagccgt aacgcccagc
    15121 aaatcgccga tcgtgccagc aagctgtacg acaagatgcg tttgttcatc gatgacatgt
    15181 ccgcgattgg tcaaagtctc gacaaagcgc aggataacta ccgccaggca atgaaaaaac
    15241 tctcttcagg tcgcggaaat gtgctggcgc aggcagaagc gtttcgcggt ttaggagtag
    15301 aaattaaacg cgagattaat ccggatttgg ctgaacaggc ggtgagccag gatgaagagt
    15361 atcgacttcg gtcggttccg gagcagccga atgatgaagc ttatcaacgc gatgatgaat
    15421 ataatcagca gtcgcgctag cccattaggg gtagttaagc cgggtagaaa tctagggcat
    15481 cgacgcccaa tctgttacac ttctggaaca attttttgat gagcaggcat tgagatggtg
    15541 gataagtcac aagaaacgac gcactttggt tttcagaccg tcgcgaagga acaaaaagcg
    15601 gatatggtcg cccacgtttt ccattccgtg gcatcaaaat acgatgtcat gaatgatttg
    15661 atgtcatttg gtattcatcg tttgtggaag cgattcacga ttgattgcag cggcgtacgc
    15721 cgtgggcaga ccgtgctgga tctggctggt ggcaccggcg acctgacagc gaaattctcc
    15781 cgcctggtcg gagaaactgg caaagtggtc cttgctgata tcaatgaatc catgctcaaa
    15841 atgggacgcg agaagctgcg taatatcggt gtgattggca acgttgagta tgttcaggcg
    15901 aacgctgagg cgctgccgtt cccggataac acctttgatt gcatcaccat ttcgtttggt
    15961 ctgcgtaacg tcaccgacaa agataaagca ctgcgttcaa tgtatcgcgt gctgaaaccc
    16021 ggcggccgcc tgctggtgct tgagttctcg aagccaatta tcgagccgct gagcaaagcc
    16081 tatgatgcat actccttcca tgtgctgccg cgtattggct cactggtcgc gaacgacgcc
    16141 gacagctacc gttatctggc agaatccatc cgtatgcatc ccgatcagga taccctgaaa
    16201 gccatgatgc aggatgccgg attcgaaagt gtcgactact acaatctgac ggcaggggtt
    16261 gtggcgctgc atcgtggtta taagttctga caggagaccg gaaatgcctt ttaaaccttt
    16321 agtgacggca ggaattgaaa gtctgctcaa caccttcctg tatcgctcac ccgcgctgaa
    16381 aacagcccgc tcgcgtctgc tgggtaaagt attgcgcgtg gaggtaaaag gcttttcgac
    16441 gtcattgatt ctggtgttca gcgaacgcca ggttgatgta ctgggcgaat gggcaggcga
    16501 tgctgactgc accgttatcg cctacgccag tgtgttgccg aaacttcgcg atcgccagca
    16561 gcttaccgca ctgattcgca gtggtgagct ggaagtgcag ggcgatattc aggtggtgca
    16621 aaacttcgtt gcgctggcag atctggcaga gttcgaccct gcggaactgc tggcccctta
    16681 taccggtgat atcgccgctg aaggaatcag caaagccatg cgcggaggcg caaagttcct
    16741 gcatcacggc attaagcgcc agcaacgtta tgtggcggaa gccattactg aagagtggcg
    16801 tatggcaccc ggtccgcttg aagtggcctg gtttgcggaa gagacggctg ccgtcgagcg
    16861 tgctgttgat gccctgacca aacggctgga aaaactggag gctaaatgac gccaggtgaa
    16921 gtacggcgcc tatatttcat cattcgcact tttttaagct acggacttga tgaactgatc
    16981 cccaaaatgc gtatcaccct gccgctacgg ctatggcgat actcattatt ctggatgcca
    17041 aatcggcata aagacaaact tttaggtgag cgactacgac tggccctgca agaactgggg
    17101 ccggtttgga tcaagttcgg gcaaatgtta tcaacccgcc gcgatctttt tccaccgcat
    17161 attgccgatc agctggcgtt attgcaggac aaagttgctc cgtttgatgg caagctggcg
    17221 aagcagcaga ttgaagctgc aatgggcggc ttgccggtag aagcgtggtt tgacgatttt
    17281 gaaatcaagc cgctggcttc tgcttctatc gcccaggttc ataccgcgcg attgaaatcg
    17341 aatggtaaag aggtggtgat taaagtcatc cgcccggata ttttgccggt cattaaagcg
    17401 gatctgaaac ttatctaccg tctggctcgc tgggtgccgc gtttgctgcc ggatggtcgc
    17461 cgtctgcgcc caaccgaagt ggtgcgcgag tacgaaaaga ccttgattga tgaactgaat
    17521 ttgctgcggg aatctgccaa cgccattcag cttcggcgca attttgaaga cagcccgatg
    17581 ctctacatcc cggaagttta ccctgactat tgtagtgaag ggatgatggt gatggagcgc
    17641 atttacggca ttccggtgtc tgatgttgcg gcgctggaga aaaacggcac taacatgaaa
    17701 ttgctggcgg aacgcggcgt gcaggtgttc ttcactcagg tctttcgcga cagctttttc
    17761 catgccgata tgcaccctgg caacatcttc gtaagctatg aacacccgga aaacccgaaa
    17821 tatatcggca ttgattgcgg gattgttggc tcgctaaaca aagaagataa acgctatctg
    17881 gcagaaaact ttatcgcctt ctttaatcgc gactatcgca aagtggcaga gctacacgtc
    17941 gattctggct gggtgccacc agataccaac gttgaagagt tcgaatttgc cattcgtacg
    18001 gtctgtgaac ctatctttga gaaaccgctg gccgaaattt cgtttggaca tgtactgtta
    18061 aatctgttta atacggcgcg tcgcttcaat atggaagtgc agccgcaact ggtgttactc
    18121 cagaaaaccc tgctctatgt cgaaggggta ggacgccagc tttatccgca gctcgattta
    18181 tggaaaacag cgaagccttt cctggagtcg tggattaaag atcaggtcgg tattcctgct
    18241 ctggtgagag catttaaaga aaaagcgccg ttctgggtcg aaaaaatgcc agaactgcct
    18301 gaactggttt acgacagttt gcgccagggc aagtatttac agcacagtgt tgataagatt
    18361 gcccgcgagc ttcagtcaaa tcatgtacgt cagggacaat cgcgttattt tctcggaatt
    18421 ggcgctacgt tagtattaag tggcacattc ttgttggtca gccgacctga gtgggggctg
    18481 atgcccggct ggttaatggc aggtggtctg atcgcctggt ttgtcggttg gcgcaaaaca
    18541 cgctgatttt ttcatcgctc aaggcgggcc gtgtaacgta taatgcggct ttgtttaatc
    18601 atcatctacc acagaggaac atgtatgggt ggtatcagta tttggcagtt attgattatt
    18661 gccgtcatcg ttgtactgct ttttggcacc aaaaagctcg gctccatcgg ttccgatctt
    18721 ggtgcgtcga tcaaaggctt taaaaaagca atgagcgatg atgaaccaaa gcaggataaa
    18781 accagtcagg atgctgattt tactgcgaaa actatcgccg ataagcaggc ggatacgaat
    18841 caggaacagg ctaaaacaga agacgcgaag cgccacgata aagagcaggt gtaatccgtg
    18901 tttgatatcg gttttagcga actgctattg gtgttcatca tcggcctcgt cgttctgggg
    18961 ccgcaacgac tgcctgtggc ggtaaaaacg gtagcgggct ggattcgcgc gttgcgttca
    19021 ctggcgacaa cggtgcagaa cgaactgacc caggagttaa aactccagga gtttcaggac
    19081 agtctgaaaa aggttgaaaa ggcgagcctc actaacctga cgcccgaact gaaagcgtcg
    19141 atggatgaac tacgccaggc cgcggagtcg atgaagcgtt cctacgttgc aaacgatcct
    19201 gaaaaggcga gcgatgaagc gcacaccatc cataacccgg tggtgaaaga taatgaagct
    19261 gcgcatgagg gcgtaacgcc tgccgctgca caaacgcagg ccagttcgcc ggaacagaag
    19321 ccagaaacca cgccagagcc ggtggtaaaa cctgctgcgg acgctgaacc gaaaaccgct
    19381 gcaccttccc cttcgtcgag tgataaaccg taaacatgtc tgtagaagat actcaaccgc
    19441 ttatcacgca tctgattgag ctgcgtaagc gtctgctgaa ctgcattatc gcggtgatcg
    19501 tgatattcct gtgtctggtc tatttcgcca atgacatcta tcacttggta tccgcgccat
    19561 tgatcaagca gttgccgcaa ggttcaacga tgatcgccac cgacgtggcc tcgccgttct
    19621 ttacgccgat caagctgacc tttatggtgt cgctgattct gtcagcgccg gtgattctct
    19681 atcaggtgtg ggcatttatc gccccagcgc tgtataagca tgaacgtcgc ctggtggtgc
    19741 cgctgctggt ttccagctct ctgctgtttt atatcggcat ggcattcgcc tactttgtgg
    19801 tctttccgct ggcatttggc ttccttgcca ataccgcgcc ggaaggggtg caggtatcca
    19861 ccgacatcgc cagctattta agcttcgtta tggcgctgtt tatggcgttt ggtgtctcct
    19921 ttgaagtgcc ggtggcaatt gtgctgctgt gctggatggg gattacctcg tcagaagact
    19981 tacgcaaaaa acgcccgtat gtgctggttg gtgcattcgt tgtcgggatg ttgctgacgc
    20041 cgccggatgt cttctcgcaa acgctattgg cgatcccgat gtactgtctg tttgaaatcg
    20101 gtgtcttctt ctcacgcttt tacgttggta aagggcgaaa tcgggaagag gaaaacgacg
    20161 ctgaagcaga aagcgaaaaa actgaagaat aaattcaacc gcccgtcagg gcggttgtca
    20221 tatggagtac aggatgtttg atatcggcgt taatttgacc agttcgcaat ttgcgaaaga
    20281 ccgtgatgat gttgtagcgc gcgcttttga cgcgggagtt aatgggctac tcatcaccgg
    20341 cactaacctg cgtgaaagcc agcaggcgca aaagctggcg cgtcagtatt cgtcctgttg
    20401 gtcaacggcg ggcgtacatc ctcacgacag cagccagtgg caagctgcga ctgaagaagc
    20461 gattattgag ctggccgcgc agccagaagt ggtggcgatt ggtgaatgtg gtctcgactt
    20521 taaccgtaac ttttcgacgc cggaagagca ggaacgcgct tttgttgctc agctacgcat
    20581 tgccgcagaa ttaaacatgc cggtatttat gcactgtcgc gatgcccacg agcggtttat
    20641 gacattgctg gagccgtggc tggataaact gcctggtgcg gttcttcatt gctttaccgg
    20701 cacacgcgaa gagatgcagg cgtgcgtggc gcgtggaatt tatatcggca ttaccggttg
    20761 ggtttgcgat gaacgacgcg ggctggagct gcgggaattg ttgccgttga ttccggcgga
    20821 gaaattgctg atcgaaacag atgcgccgta tctgctcccg cgcgatctca cgccaaagcc
    20881 atcatcccgg cgcaacgagc cagcccatct gccccatatt ttgcaacgta ttgcgcactg
    20941 gcgtggagaa gatgccgcat ggctggctgc caccacggat gccaatgtca aaacactgtt
    21001 tgggattgcg ttttagagtt tgcggaactc ggtgttcttc acactgtgct taatctcttt
    21061 attaataaga ttaagcaata gcatggagcg agcctcacca tcgggttcgg tgaaaatggc
    21121 ctgaaagcct tcgaacgcgc cttcggtaat aatcacctta tcacccggat aagggggggc
    21181 cggatcgaca atgtctttcg gtttatatac cgatagctga tgaattaccg ccgatgggac
    21241 tatcgctggc gacgcgccaa agcgcacaaa gtggctgacg ccacgcgtag cgctgatggt
    21301 cgtggtatga atcacttctg gatcaaattc cacaaacagg tagttaggga ataacggttc
    21361 actgactgcg gtacgtttac cccgtacgat tttttccagg gtgatcatcg gagcgaggca
    21421 attaaccgcc tgtctctcaa ggtgttcctg ggcacgttga agttgcccgc gcttgcagta
    21481 cagtaaatac caggattgca taatgactct tatccgttta atcggggcgc aaggatagca
    21541 aaagctttac gctaagttaa ttatattccc cggtttgcgt tataccgtca gagttcacgc
    21601 taatttaaca aatttacagc attgcaaaga tgaacgccgt ataatgggcg cagattaaga
    21661 ggctacaatg gacgccatga aatataacga tttacgcgac ttcctgacgc tgcttgaaca
    21721 gcagggtgag ctaaaacgta tcacgctccc ggtggatccg catctggaaa tcactgaaat
    21781 tgctgaccgc actctgcgtg ctggtgggcc tgcgctgttg ttcgaaaacc ctaaaggcta
    21841 ctcaatgccg gtgctgtgca acctgttcgg tacgccaaag cgcgtggcga tgggcatggg
    21901 gcaggaagat gtttcggcgc tgcgtgaagt tggtaaatta ttggcgtttc tgaaagagcc
    21961 ggagccgcca aaaggtttcc gcgacctgtt tgataaactg ccgcagttta agcaggtgtt
    22021 aaacatgccg acaaagcgac tgcgtggtgc gccctgccaa caaaaaatcg tctctggcga
    22081 tgacgtcgat ctcaatcgca ttcccattat gacctgctgg ccggaagatg ccgcgccgct
    22141 gattacctgg gggctgaccg taacgcgcgg cccgcataaa gagcggcaga atctgggcat
    22201 ttatcgccag cagctgattg gtaaaaacaa actgattatg cgctggctgt cgcatcgcgg
    22261 cggcgcgctg gattatcagg agtggtgtgc ggcgcatccg ggcgaacgtt tcccggtttc
    22321 tgtggcgctg ggtgccgatc ctgccacgat tctcggtgca gtcacccccg ttccggatac
    22381 gctttcagag tatgcgtttg ccggattgct acgcggcacc aaaaccgaag tagtaaagtg
    22441 tatttccaat gacctcgaag tgccagccag tgccgaaatc gtcctggaag ggtatatcga
    22501 tcctggtgag atggcgccgg aagggccgta tggtgaccac acaggttact ataatgaagt
    22561 cgataatttc ccggtgttta ccgtgacgca tattacccag cgtgaagatg cgatttacca
    22621 ttccacctat accgggcgtc cgccagatga gcccgcggta ctgggcgtgg cgttgaacga
    22681 agtgtttgta ccgattctgc aaaaacagtt cccggaaatt gtcgattttt acctgccgcc
    22741 ggaaggctgt tcttatcgtc tggcggtagt gacgatcaaa aaacagtacg ccggacacgc
    22801 gaagcgcgtc atgatgggcg tctggtcgtt cttacgccag tttatgtaca ctaaatttgt
    22861 gatcgtttgc gatgatgacg tcaacgcacg cgactggaac gatgtgattt gggcgattac
    22921 cacccgtatg gacccggcgc gggatactgt tctggtagaa aatacgccta ttgattatct
    22981 ggattttgcc tcgcctgtct ctgggctggg ttcaaaaatg gggctggatg ccacgaataa
    23041 atggccgggg gaaacccagc gtgaatgggg acgtcccatc aaaaaagatc cagatgttgt
    23101 cgcgcatatt gacgccatct gggatgaact ggctattttt aacaacggta aaagcgcctg
    23161 atgcgcgttt gttttgccct atttatcgat ccgacagaga aagcgcatga caaccttaag
    23221 ctgtaaagtg acctcggtag cagctatcac ggataccgta tatcgtgtcc gcatcgtgcc
    23281 agacgcggcc ttttcttttc gtgctggtca gtatttgatg gtagtgatgg atgagcgcga
    23341 caaacgtccg ttctcaatgg cctcgacgcc ggatgaaaaa gggtttatcg agctgcatat
    23401 tggcgcttct gaaatcaacc tttacgcgaa agcagtcatg gaccgcatcc tcaaagatca
    23461 tcaaatcgtg gtcgaccttc cccacggaga agcgtggctg cgcgatgatg aagagcgtcc
    23521 gatgattttg attgcgggcg gcaccgggtt ctcttatgcc cgctcgattt tgctgacagc
    23581 gttggcgcgt aacccaaacc gtgatatcac catttactgg ggcgggcgtg aagagcagca
    23641 tctgtatgat ctctgcgagc ttgaggcgct ttcgttaaag caccttggtc tgcaagtggt
    23701 gccggtggtt gaacaaccgg aagcaggctg gcgtgggcgt actggtaccg tgttaacggc
    23761 ggtattgcag gatcatggta cgctggcaga gcatgatatc tatattgccg gacgttttga
    23821 gatggcgaaa attgcccgtg acctgttttg cagtgagcgt aatgcgcggg aagatcgcct
    23881 gtttggcgat gcgtttgcat ttatctgaga tataaaaaaa cccgcccctg acaggcggga
    23941 agaacggcaa ctaaaacttt attcagtggc atttagatct atgtcgcatc cggcaattgg
    24001 tgcacgatgc ctgatgcgac gctggcgcgt cttatcaggc ctacattggt gccggatcgg
    24061 tagaccggat aaggcgttca cgccgcatcc ggcaagtggt taaacccgct caaacaccgt
    24121 cgcaataccc tgacccagac cgatacacat cgtcgccaga ccaaactgaa cgtctttgcg
    24181 ttccatcaga ttcagcagcg tggtgctgat acgcgcaccg gaacaaccca gcggatgacc
    24241 cagcgcgatc gcaccaccat tgaggttgat cttctcgtca atctgctcca ttagcccgag
    24301 atctttaatg cacggcagga tctgcgcggc aaaggcttcg ttcatctcaa acacgccgat
    24361 atcgctggca gaaagccccg cttttttcag cgccagcttc gaggccggaa ccgggccgta
    24421 acccataatc gatgggtcac aaccaacgac cgccatcgaa cgcacacgag cgcgcggctt
    24481 aagacctaat tcatgggcgc ggctttcact catcaccagc atggcagctg cgccatcgga
    24541 aagtgcagaa gatgtgcccg ccgttaccgt accgtttact ggatcaaacg ccggacgcag
    24601 cgtggcgagg gcttccacgg tggtttccgg gcgaatcact tcgtcgtaat taaactgctt
    24661 caggacgccg tcggcatcgt gaccaccagt tgggatgatt tcatttttaa atgcggccga
    24721 ctgcgtggcg gcccaggcgc gggcgtgtga ccgcgcggca aaggcatcct gcatttcacg
    24781 gctgataccg tgcatacgcg ccagcatttc tgccgttaag cccatcatgc ccgccgcttt
    24841 ggcgacattg cggctcaggc cggggtgaaa atcgacgccg tgactcatcg gcacatggcc
    24901 tatatgctcc acgccgccaa ccagacatgc ctgcgtatcg ccagtcatga tcattcgtgc
    24961 tgcgtcatgc agtgcctgca tggatgaacc acacaagcga ttgacggtaa ccgccgggac
    25021 agagtgcggc acttctgcca gcagcgccgc gttacgggcg atgttaaaac cctgctccag
    25081 cgtctgttgc acacaacccc agtaaatatc gtcgagggcc gccgcttcca gcgccgggtt
    25141 acgcgccagc aggctacgca ttaaatgagc ggagagatct tccgcacgca cgttacgaaa
    25201 agcaccgccc ttcgaacggc ccatcggggt gcgaattgca tcgacaatga caacctgttc
    25261 cattgtgact ccttaagccg ttttcaggtc gccaaccgga cgggctggct caaccggagg
    25321 atagtacggt tcgttatgac gcgctttatt acgcagacct tccggcactt catacagcgg
    25381 gccgaggtgc tgatattgct gtgccatatc gaggtatttt gcgctaccga gggtgtccag
    25441 ccagcggaac gcgccgccgt ggaacggagg gaagcccagg ccgtagacca gcgccatatc
    25501 cgcttccgcc ggagtggcga taatgccctc ttccagacag cgcaccactt cgttgaccat
    25561 cgggatcatc atgcgggcga taatctcttc ttcgctgaaa tcgcgcttcg gttggctcac
    25621 ttctgccagc aggtcttcaa cggcggcgtc ttcttctttc ttcggcttac ctttgctgtc
    25681 ttctttataa cgccagaaac cgaggccgtt cttctgacca aagcggttgg catcaaacag
    25741 cgcgtcgatg gcatcgcggt aatctttctg catccgctgc gggaagcctg ctgccatgac
    25801 agcctgagcg tgatgcgcgg tatcaatgcc cacaacgtcc agcagatatg ccgggcccat
    25861 cggccagcca aactgttttt ccatcacttt gtcgatcttg cggaaatccg cgccgtcgcg
    25921 cagcagctgg ctgaaaccgg cgaaatacgg gaacagcacg cggttaacaa agaagccggg
    25981 gcagtcgtta accacaatcg gcgtcttgcc catcttgctc gcccaggcga caactttcgc
    26041 gatggtttcg tcggagcttt tctcgccgcg aataatttct accaacggca ttcggtggac
    26101 cgggttaaag aagtgcatcc cgcagaagtt ttccgggcgt tccagcgcgt tggccagttc
    26161 gctgatagga atggttgaag tgttagacgc cagcacggta tcctggcgta ctttttgttc
    26221 ggtttctgcc agtacggctt ttttcacttt cgggttttca acaaccgctt ctaccacaat
    26281 atccacgcgg tcaaatccgg cgtagtcgag cgttgggtgg atcgtggaga tcacgccagc
    26341 cagtttcaga ccatcgattt tgccgcgctc aagctgcttg ttcagcagtt tcgcggcttc
    26401 ggtcatgccg agggttaacg acttgtcgtt gatatctttc atgacaaccg gcacgccttt
    26461 ccacgcggac tggtaagcga taccgccacc catgatgcct gcgcccagca cagcagcctg
    26521 tttcggggtt tcaacgtctt tggtgagttt cttcgctttg ccttttacat attgatcgtt
    26581 aaggaaaatg ccgaccagtg cgcgagcttc gttggtatgc gccagcggga caaaactttt
    26641 gttttccaga tttaaggctt cttcacgacc aaaacgggcc gcagcttcaa tggtttttac
    26701 tgcggtgatg ggggccggat aatgtttccc cgccgtttgc gcgaccattc ctttagcgat
    26761 ggtgaagctc atggtggctt caatcttgct cagttttagc ggttcctgct tcggctgacg
    26821 tttcgctttc cagtcgaggt cgccgttaat ggcctggcgt aaaatcgcca tcgccccttc
    26881 aatcagtttt tctgctttga ctacgccatc caccagaccg attttcagcg cctgatccgc
    26941 gccgacatct ttaccggcgg caatgatttc cagtgcactg tcagcgccca gcatacgtgg
    27001 catacgtaca gaaccgccaa agcccggcat gatgcccagt ttggtttccg gcagaccgat
    27061 gcgcagatcc ggcgtcgcca gacgataatc ggtcgccagc acgcattcgc agccaccacc
    27121 cagcgcatag ccgttgacgg cagcaatggt cggcaccggc agatcttcca ggcgattaaa
    27181 cacgctattg gcaaagtgca gccactgact taactgttct tcaggaacga ggaacaggga
    27241 caaaaattcg gtgatatcag caccgacgat aaaggctgct ttgttcgaac gcagcagcag
    27301 cccttttaga tctgattgct gttccagcac gccgatggcc tcgccgaggc tggcgacggt
    27361 cgcagtgtcg agtttattaa ctgaacctgg ggcatcaaat accagttcgg caatgccatc
    27421 ttccagccag tcaaggtaca gggtgtcgcc tttgtaaagc atgtcagtct cctgaatccg
    27481 caaggtaatc tggtcgtacc agatgcatcg gagtgtgtgt tttgtgttaa aaatatgcaa
    27541 ataaaagatt aaagaaatgc cgatctgatc acgctcggca gaaatcacgc tctggatgaa
    27601 cgatgtgcta agatgcggag acttaaggtc aaaaaaacag aagggtaaaa aatggaatca
    27661 ctggcctcgc tctataaaaa tcatatagct accttacaag aacggactcg cgatgcgctg
    27721 gcgcgcttca agctggatgc attacttatt cactccggcg agctgttcaa cgtttttctc
    27781 gacgatcatc cctatccgtt taaagtgaac ccgcaattca aagcgtgggt gccggtaact
    27841 caggtgccaa actgctggtt gctggtggat ggcgtgaaca agccgaaact gtggttctat
    27901 ctgccggttg attactggca caacgtcgaa ccgctgccga cctccttctg gactgaagat
    27961 gtggaagtga tcgcgctgcc gaaagccgat ggcattggta gcctgctgcc tgctgcgcgc
    28021 ggcaatatcg gttatatcgg tccggtgccg gaacgtgcgc tgcaactggg tattgaggcc
    28081 agcaacatca acccgaaagg ggtgatcgac tacctgcatt attaccgctc cttcaaaacc
    28141 gagtacgaac tggcctgtat gcgtgaagcg cagaaaatgg cggtcaatgg tcatcgtgcg
    28201 gcagaagaag cgttccgttc tggcatgagc gagttcgata tcaatattgc ctatctgacc
    28261 gcgaccggtc atcgtgatac cgacgtacct tacagcaaca ttgtggcgct taacgaacac
    28321 gctgcggtgt tgcattacac caaactggac caccaggcac cggaagagat gcgcagcttc
    28381 ctgctggatg ccggggccga atataacggc tatgcggctg acctgacccg tacctggtcg
    28441 gcaaaaagcg acaacgacta cgcacagctg gtgaaagacg taaatgatga acaactggcg
    28501 ctgatcgcca ccatgaaagc aggcgtcagc tatgtggatt accacatcca gttccatcag
    28561 cgcatcgcca aactgctgcg taaacatcaa atcatcaccg atatgagtga agaagcgatg
    28621 gtcgaaaacg atctcaccgg accgtttatg ccgcacggta ttggccatcc gctgggcctg
    28681 caggtgcatg acgtcgccgg ttttatgcag gatgatagcg gtacacacct cgcggcaccg
    28741 gcaaaatatc cgtacctgcg ctacacccgt attctccagc cgggcatggt gttaaccatc
    28801 gaaccgggta tctacttcat tgaatcgctt ctggcaccgt ggcgtgaagg gcagttcagc
    28861 aagcacttca actggcagaa aattgaagca ctgaaaccgt tcggcggcat tcgtatcgaa
    28921 gacaacgtgg tgatccacga aaacaacgtg gaaaacatga cccgggatct gaaactggcg
    28981 tgatggaaag ctggttaatt cctgcggcac cggtcacggt cgttgaagag atcaaaaaga
    29041 gccgcttcat aacgatgttg gcgcataccg atggcgttga ggcggcgaaa gcgtttgttg
    29101 aatcggtgcg ggcagaacac cccgatgccc gccaccattg cgtggcgtgg gtggcgggtg
    29161 cgccggatga ttctcaacag ctgggtttct ctgacgacgg ggagccggcg ggaacggcag
    29221 gtaaaccgat gctcgcccag ctaatgggca gcggcgtcgg ggaaattacc gctgtggtag
    29281 tgcgctacta cggcggcata ttgcttggca ccggtgggtt agtgaaagcg tatggcggcg
    29341 gcgtgaatca ggcgctgcgc cagctaacga cccaacgcaa gacgccatta accgaatata
    29401 ctttgcaatg tgaatatagt cagttaaccg gcattgaagc gttgctgggg cagtgtgacg
    29461 gcaaaattat caacagtgat tatcaggcat tcgttctgct gcgggtggcg cttccggcgg
    29521 cgaaagtggc tgaattttcc gcaaagctgg cggattttag ccgtggttca ttgcaattgt
    29581 tagcgattga agaataatcc ccacttcgtt ttgcagaact aaggaagcgg cagagatgca
    29641 ttttcgcgcc attacccgaa tcgttggact actggtcatc ttattttcag ggaccatgat
    29701 tatccctggg ctggtagcac tcatctaccg ggatggagcg ggccgcgctt ttacccagac
    29761 cttttttgtc gccctcgcca ttggctctat gctgtggtgg ccgaaccgca aagagaaggg
    29821 cgaactgaaa tcccgtgagg ggtttctgat agtggtgctg ttttggaccg tgctgggtag
    29881 tgtcggtgcg ctccctttta tcttctcgga aagcccgaac ctcacgatta ccgatgcgtt
    29941 ttttgaatct ttctctggcc tgaccaccac cggggccact acgctggtgg ggctggattc
    30001 gctccctcat gccatcctct tttatcgcca gatgctgcaa tggtttggcg ggatggggat
    30061 catcgtgtta gcggttgcga tactgcctat cctcggcgtg ggtgggatgc agctctatcg
    30121 cgcagaaatg cccggcccgc tgaaagataa caaaatgcgc ccgcgaattg cggaaacggc
    30181 gaaaactctg tggttgattt atgtcttgct gaccgtcgcc tgtgcgctgg cgttgtggtt
    30241 tgccggaatg gatgcctttg atgccatcgg ccatagcttt gcgactatcg ctattggcgg
    30301 cttctcgaca catgatgcca gtatcggtta tttcgacagt ccgactatta acactatcat
    30361 tgctatcttc ctgctgatct ccggctgtaa ctacggtctg cacttttcac tgttaagtgg
    30421 gcgtagtctg aaggtttatt ggcgcgatcc ggaatttcgc atgtttatcg gcgtacagtt
    30481 tacgctggtg gttatttgta cactcgtact gtggtttcat aatgtctaca gttcggcgct
    30541 gatgacaatt aaccaggcgt ttttccaggt ggtatcgatg gcgacaaccg ccgggtttac
    30601 gactgacagc attgcccgct ggccgctctt tttgccggta ctgcttttat gttcagcttt
    30661 tatcggcggt tgcgccgggt caacgggcgg tggcctgaaa gtgatccgca tcctgctgct
    30721 gtttaagcag gggaaccgtg agctgaaacg actggtgcat ccgaacgccg tctatagcat
    30781 taagctgggg aatcgcgcac tgccggaacg tatcctcgaa gccgtttggg gatttttctc
    30841 cgcctatgca ttggtgttta ttgtcagtat gctggcgatt atcgccacgg gcgtggatga
    30901 tttttctgcc tttgcctcag ttgttgcgac attgaataac ctggggccag ggcttggcgt
    30961 ggttgctgat aactttacca gtatgaaccc ggtggctaaa tggatcctga ttgccaacat
    31021 gctgtttggt cgtctcgagg tctttacatt actggtgctc tttaccccga ctttctggcg
    31081 tgaatgatgg agtaatacgt gaaaacatta attcttttct caacaaggga cggacaaacg
    31141 cgcgagattg cctcctacct ggcttcggaa ctgaaagaac tggggatcca gacggatgtc
    31201 gccaatgtgc accgcattga agaaccacaa tgggaaaact atgaccgtgt ggtcattggt
    31261 gcttctattc gctatggtca ctaccactct gcgttccagg aatttgtcaa aaaacatgcg
    31321 acgcgactga attcgatgcc gagcgccttt tattccgtaa acctggtggc gcgcaaaccg
    31381 gagaagcgta ctccacagac caacagctac gcgcggaagt ttctgatgaa ctcgcaatgg
    31441 cgtcccgatc gctgcgcggt cattgccggg gcgctgcgtt acccacgtta tcgctggtac
    31501 gaccgtttta tgctcaagct gattatgaag atgtcaggcg gtgaaacgga tacgcgcaaa
    31561 gaagttgtct ataccgattg ggagcaggtg gcgaatttcg cccgagaaat cgcccattta
    31621 accgacaaac cgacgctgaa ataagcataa agaataaaaa atgcgcggtc agaaaattat
    31681 tttaaatttc ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga
    31741 acaacggcaa acacgcagcc gggtcagcgg ggttctcctg agaattccga cagagaaagc
    31801 aaaaataaat gcttgacttt gtagcgggaa agcgtattat gaacaccccg cgc
//
LOCUS       DAZPEU010000026        31418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPEU010000026 DAZPEU010000000
VERSION     DAZPEU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.026"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(248..1105)
                     /gene="repA"
                     /locus_tag="ACHU2L_001131"
     CDS             complement(248..1105)
                     /gene="repA"
                     /locus_tag="ACHU2L_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR1356076.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(1098..1172)
                     /gene="tap"
                     /locus_tag="ACHU2L_001132"
     CDS             complement(1098..1172)
                     /gene="tap"
                     /locus_tag="ACHU2L_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR1356077.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(1410..1664)
                     /locus_tag="ACHU2L_001133"
     CDS             complement(1410..1664)
                     /locus_tag="ACHU2L_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR1356078.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
     gene            complement(1904..2494)
                     /locus_tag="ACHU2L_001134"
     CDS             complement(1904..2494)
                     /locus_tag="ACHU2L_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1356079.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(2532..2741)
                     /gene="hha"
                     /locus_tag="ACHU2L_001135"
     CDS             complement(2532..2741)
                     /gene="hha"
                     /locus_tag="ACHU2L_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1356080.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(2787..3248)
                     /locus_tag="ACHU2L_001136"
     CDS             complement(2787..3248)
                     /locus_tag="ACHU2L_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1356081.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(3493..3705)
                     /locus_tag="ACHU2L_001137"
     CDS             complement(3493..3705)
                     /locus_tag="ACHU2L_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1356082.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(3834..4394)
                     /locus_tag="ACHU2L_001138"
     CDS             complement(3834..4394)
                     /locus_tag="ACHU2L_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1356083.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            complement(4497..5357)
                     /locus_tag="ACHU2L_001139"
     CDS             complement(4497..5357)
                     /locus_tag="ACHU2L_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1356084.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(5416..6162)
                     /gene="traX"
                     /locus_tag="ACHU2L_001140"
     CDS             complement(5416..6162)
                     /gene="traX"
                     /locus_tag="ACHU2L_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR1356085.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            complement(6182..11452)
                     /gene="traI"
                     /locus_tag="ACHU2L_001141"
     CDS             complement(6182..11452)
                     /gene="traI"
                     /locus_tag="ACHU2L_001141"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR1356086.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREV
                     ARENLLQERLQQIERDMVRDLQKEKTLGGD"
     gene            complement(11452..13659)
                     /gene="traD"
                     /locus_tag="ACHU2L_001142"
     CDS             complement(11452..13659)
                     /gene="traD"
                     /locus_tag="ACHU2L_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR1356087.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQ
                     PQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPP
                     GISESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(13710..14447)
                     /locus_tag="ACHU2L_001143"
     CDS             complement(13710..14447)
                     /locus_tag="ACHU2L_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356088.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(14650..15381)
                     /gene="traT"
                     /locus_tag="ACHU2L_001144"
     CDS             complement(14650..15381)
                     /gene="traT"
                     /locus_tag="ACHU2L_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR1356089.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(15430..15915)
                     /locus_tag="ACHU2L_001145"
     CDS             complement(15430..15915)
                     /locus_tag="ACHU2L_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFR1356090.1"
                     /translation="MIMRCLTHITLVTVIQFIACYLASWGNAETIFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYD
                     E"
     gene            complement(15931..18753)
                     /gene="traG"
                     /locus_tag="ACHU2L_001146"
     CDS             complement(15931..18753)
                     /gene="traG"
                     /locus_tag="ACHU2L_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR1356091.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            complement(18750..20123)
                     /gene="traH"
                     /locus_tag="ACHU2L_001147"
     CDS             complement(18750..20123)
                     /gene="traH"
                     /locus_tag="ACHU2L_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR1356092.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(20110..20502)
                     /gene="trbF"
                     /locus_tag="ACHU2L_001148"
     CDS             complement(20110..20502)
                     /gene="trbF"
                     /locus_tag="ACHU2L_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR1356093.1"
                     /translation="MKEKRNDAELKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRREKKLREGGNNASH"
     gene            complement(20483..20830)
                     /gene="trbJ"
                     /locus_tag="ACHU2L_001149"
     CDS             complement(20483..20830)
                     /gene="trbJ"
                     /locus_tag="ACHU2L_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR1356094.1"
                     /translation="MPPVLWRGWIPFCRCTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMNGLNESS
                     EISEETYNERKKK"
     gene            complement(20760..21305)
                     /gene="trbB"
                     /locus_tag="ACHU2L_001150"
     CDS             complement(20760..21305)
                     /gene="trbB"
                     /locus_tag="ACHU2L_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR1356095.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGTK"
     gene            complement(21292..21576)
                     /gene="traQ"
                     /locus_tag="ACHU2L_001151"
     CDS             complement(21292..21576)
                     /gene="traQ"
                     /locus_tag="ACHU2L_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR1356096.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            complement(21695..22039)
                     /gene="trbA"
                     /locus_tag="ACHU2L_001152"
     CDS             complement(21695..22039)
                     /gene="trbA"
                     /locus_tag="ACHU2L_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR1356097.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSSAWSG
                     VMWFFIRGLMTG"
     gene            complement(22053..22796)
                     /gene="traF"
                     /locus_tag="ACHU2L_001153"
     CDS             complement(22053..22796)
                     /gene="traF"
                     /locus_tag="ACHU2L_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR1356098.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(22789..23046)
                     /locus_tag="ACHU2L_001154"
     CDS             complement(22789..23046)
                     /locus_tag="ACHU2L_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR1356099.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(23073..24923)
                     /gene="traN"
                     /locus_tag="ACHU2L_001155"
     CDS             complement(23073..24923)
                     /gene="traN"
                     /locus_tag="ACHU2L_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR1356100.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(24920..25558)
                     /gene="trbC"
                     /locus_tag="ACHU2L_001156"
     CDS             complement(24920..25558)
                     /gene="trbC"
                     /locus_tag="ACHU2L_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR1356101.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(25567..25872)
                     /locus_tag="ACHU2L_001157"
     CDS             complement(25567..25872)
                     /locus_tag="ACHU2L_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356102.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVIVPVILFYF
                     APDSFLAEIIHEWKNADISEQVTLVRLYLKVTFTCAFVAVFLNYLFSDEFMPVNSRK"
     gene            complement(25902..26894)
                     /gene="traU"
                     /locus_tag="ACHU2L_001158"
     CDS             complement(25902..26894)
                     /gene="traU"
                     /locus_tag="ACHU2L_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR1356103.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(26891..27523)
                     /gene="traW"
                     /locus_tag="ACHU2L_001159"
     CDS             complement(26891..27523)
                     /gene="traW"
                     /locus_tag="ACHU2L_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR1356104.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(27520..27906)
                     /gene="trbI"
                     /locus_tag="ACHU2L_001160"
     CDS             complement(27520..27906)
                     /gene="trbI"
                     /locus_tag="ACHU2L_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR1356105.1"
                     /translation="MTITQKTTDVTAPRRSHWWWTVPGYLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(27903..30533)
                     /gene="traC"
                     /locus_tag="ACHU2L_001161"
     CDS             complement(27903..30533)
                     /gene="traC"
                     /locus_tag="ACHU2L_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR1356106.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILRQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(30659..31006)
                     /locus_tag="ACHU2L_001162"
     CDS             complement(30659..31006)
                     /locus_tag="ACHU2L_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356107.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(31034..31252)
                     /locus_tag="ACHU2L_001163"
     CDS             complement(31034..31252)
                     /locus_tag="ACHU2L_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356108.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
BASE COUNT         7228 a         8968 c         7359 g         7863 t
ORIGIN      
        1 tggcggctgg cgctttatga catgaacggt tgtaacctta tggggaagtc ccttgcagtt
       61 aaatgtggat aagcaaaatt ccccgtctgt gaggcgtgtt ttgtatcgaa aacagggggg
      121 gccggatgca ccagaagctg gatgatgagg ttgttttttg tatgtggtgc tgattttttg
      181 tgcgctgtcg ggcttcagac gtgcggaagc ctccggcgca ggccggatta ttcagaggag
      241 gtcactttca gggggaagct gtggccagcc ggctgtaatt acggttacgt gacagaatca
      301 tgcgctcctt cacacgacgc tcaacttcgc gttttaccgc ctcacgattg gcagtgaagc
      361 gcccttccgc gatttcgcgc gtcagctgcc gtttcaccag ggtgacaata tcctgacgtt
      421 ccctgtccgc atcacgacgc gcacgggcac gctttattcc ccgggactta agctctgtct
      481 gataactgcg aaaacgctca cgaacaaaac gccaggcttt cgctatcagt tcatccatgc
      541 ccagggtatc cagcccctgc tttttgcgtt gtttgttttc ccataccaca cggctgcggc
      601 gcgcggcggc cactgcctcc tctgatacat cgagggcagc aaacagtgca gatgtgaacg
      661 tgatatcggt cggaatgtag cacccgataa gcgggtcata ttccgtctgg taggtaatca
      721 gtcccagctc tgacaggaac gtcagggccc gggtggcacg ggtgatggag agttttccgg
      781 cagcagactc cgtcgccagt ccgcactcaa tggccagcgt ggtgatggag cactggacgc
      841 ggttggccag cgggtcatag tggaaacaca gcccctgcag gagcgcatca atagcccgtc
      901 gacgcagcac tggtggcatg cgtcgacgca gaccacgcga acgggcatgc gccacatgaa
      961 tggcgaaatc aaaacgggaa gtgaagccca ccgccttttc catcagtttt tcgcagaact
     1021 tcagcgttcc tgcaccttca cggggtgtaa acaccggatt cgggttcttt acctggcggt
     1081 aatacgtttg gtgaagatca gtcacaccat cctgcactta caatgcgcag aaggagcgag
     1141 cacagaaaga agtcttgaac ttttccgggc atataactat actccccgca tagctgaatt
     1201 gttggctata cggtttaagt gggccccggt aatcttttcg tactcgccaa agttgaagaa
     1261 gattatcggg gtttttgctt ttctggctcc tgtaaatcca catcagaacc agttccttgc
     1321 caccttacgg cgtggccagc cacaaaattc cttaaacgat cagtaatcta gcactaatct
     1381 tctgaacact caagaatgta agcccatcat cacatgcatc gtttttgcgc ttcacttttt
     1441 atcagtgcgg tcagaacttc agcctgagtc agaccatctt catgacacat ttgcatgagc
     1501 atggccttat actttggttc aagaaatact tttacttcct tgaacgaagc tcttttacgg
     1561 gccactgata atctttgttt ctctgcatca gaaagcggat tcccctttct gtatgctcgt
     1621 tttgcgccag atgaggaagt cactgcattt tctgtctgcg acatctcgcc tcctcaatac
     1681 tcaaacaggg atcgtttcgc agaggatact acagtttttt gaaatcagcg acttgagaat
     1741 tgtgacgaag atctgtgttg gcgcacaaat caacggggat tactgtcgtt taatgtgatt
     1801 taaactgtga aatagtatgg ttttcagtta ttgaaacgcc gggagcgggg aaaacttgct
     1861 ttttcccgtt tccggggttg gacaactgag caacgcgaag gcgtcagcta cgatgttccg
     1921 gggactgctg atcagcccct gttgtattca gccattctcc ggtcatctgc agcagttttc
     1981 tggcatcgtg gcttctgagc aacggaatac cagcctgcct cagaacctct tccagaagaa
     2041 tatcccggcg tctgcgttcc ggtcgtaaat ggctggcatc atccagctct actgctgcaa
     2101 caatactgaa cgaacgccgc tcaacgataa ccacatcaac atgccactga gacaccatcc
     2161 tgaataactg ccaccactgg cgtgatcgtg gccggatatt cccgttcagc tggacgatat
     2221 ccgcgacccg tacctgcgga cacagatacc accgcttcat atccacaatt ctgaacagtc
     2281 ctttcataaa actgacttcc ctttctgtca gcagcgtctt ctgacgttgc cagtattcac
     2341 cgccggacag tattcgctct gcatcaccgg tccgtatacc tgccgcatgc aattgcatca
     2401 gcaaaggaga atgctgattt acacttctga ggataatccg gataagtaac gctgcaataa
     2461 tcagaacaat aagaataaaa ataatcagct tcattgttta tctgctcctg gtaattaatt
     2521 atgctgaatt attaccttac atatttccat atctcttttg gtatcctgtc atataacttt
     2581 ccggtgatta attccgctaa ccggtggtct gctgctgaat taaatgattc acgttcactg
     2641 tttaataaac tgtctctgtt tttatgaata attttttcca gcgtattgac tgatgaacat
     2701 cgccgtaact gatacagcca ttcctgtttt gttttcgcca tttttattcc cgctgagaac
     2761 agatgaatga taagattgcc atcgctttat ttacggtgtc tccatatcca ggggggaacc
     2821 ggatcagcat ctgaccagag cccacgtttt tgttgtctgg cttcgttctg aaggacaggc
     2881 agcacgggat ctgtgttgta ctgctcatat acccaggcag ccccggcccg gaccataaac
     2941 tgattaacat tcattccgtc tggtgcataa acctgcccga gaatacgacc ataacgatct
     3001 cgctgaaaat atgtgacggt aaccgttttt cctgcaacca gtgatttcat catgtccgtt
     3061 gaccaccggc cataatcctg tttcttttcc ggtgcatcaa tatttatcag cctgatccgc
     3121 actgccttcc gtgagtccat gacctcgatg gtatcaccgt ccagtacacg aacaacccgt
     3181 ccatgaatat ctgcacacaa taccggccat gaaaagataa ataataccag tggaatatat
     3241 ttcctcatgt tattctccat atcagataaa tggcacctgt atttcaggta atcctgaata
     3301 tcgcagcaca cactgaataa caccttattt tgtgaaatta atcacaaaat ataaaatcac
     3361 gacttaatac ggaaaaaact ctgtgctact cagaactgtc cggtcatact tcccgaccgg
     3421 atattaaaaa aagcaaatct catgcattat tcagccttct gaatccctgg agtaatgaag
     3481 gctgcttttt cgttacaccc ttccccggca gcgccggtgg cagtgttcag ccctttttcg
     3541 ggcaaactgc tgccagtctg tccggggagc agactgcgca gcccgtagcc atgcctcagc
     3601 ggctcccggc cagtcccccc tgacttccag ccggacagcc cggcgtccga cctgccggta
     3661 gcgccatacc ccggagtttt gcgaatcacg ccctgttccg ttcattttcc ttcccccttt
     3721 aatccaggcc ggtgaatgtg ttataaattt ttataacctt cttctgccgg gtatggaaga
     3781 aggtttcaga gaagccttat ttttccgcct cctttagtgt gaaggaggct tttttattgt
     3841 tcatcaagca cggcctgaag ttctgccttt atccggttct ggcggcggat tttatccaga
     3901 cgctctgccg catacacttc ttcctcctga gaaatatgct ccgtcacata cccttccgtg
     3961 tcataccggc aggcaccggc tttcatggca cacagatagc tttctgaacg ggtgatggcc
     4021 ttcagcgccc tgcgcagttt tttatgcgag agcgggatat tccgctgcgc cacgtcttcc
     4081 agtaacacgt cccggatacc gcaggccaga agccggggtg tgtcaccgtc aaataatccc
     4141 ggccaccagg gtttcagggt gttcacggct tcatccagtg tgggcaggtt cagataaatg
     4201 gacagcgcct gtctggcctg cgcttttttt gctgtcagct ctgcttcccg ggcagccttc
     4261 tccgccagtt tctgcttttt caccttccat tttggtggcg tggtgacatt gatgatggtt
     4321 ttccggctcc gggtcggtgt ttccccttcc gttttccgtt tcagtgtcag taccggtcgt
     4381 ttctgctctg tcatgattgc cctgaccttt gtgaaaatat gtcctccagg tcgttgaaac
     4441 tactcactat gatattaagc tgacctcaac gacctctgac atattaatac cgacaattaa
     4501 agatgttttt taaaccattg cgtctgtact gccgccacac gttcaaaaaa cgcgccttca
     4561 taaatatcat aatggcatgc gtcagcttcc tcgtataatt ccttagtacc agaagcaaca
     4621 gcatcatata aggcttttcc ctgttctggt ggattaacac tatcctggcc ggcaataaca
     4681 atcaaaactg ggcactgtac tttggctgcg ctctctgccg gtttatattg caacgtctcc
     4741 ataaccgtaa ggaaaggtat ttttatatcc atttcaggat actgaccttt taccttctcg
     4801 aagaatacct ttgattcatt atcactcagt acccgggtaa cccccacaaa catctctttc
     4861 ccggtgtttt tcttcttctc tgccatttta ttcagcgtgg acagaaaaga cgctctctcc
     4921 gattcattca tctctccggt aaccagaaca tcaccgtcag caaaagccag ctggctgaca
     4981 atacatttta cgcgttggtc ctgcgcagct gcactgaaga catgcccccc tcccagcgat
     5041 gttccccata acccaatacg ctgattatca atacattcct gcttttctgc ccaattgata
     5101 actgaaataa tatcttcggt ctgcattgca ggaaccagac gccctcgttc tccatcactc
     5161 tcaccgaatc ctcgataatc aaaagtaatc gttgcgaatc ctgcttctgt gaaggcattc
     5221 gcaaagcaag ggagcagaac attcctgata ccgcaaaacc catggcataa gataatcaaa
     5281 ggatgtttta tatttccttc aggaacacga aatgtcaggg caataccttc agagagtttg
     5341 tgatccgtaa ttttcataaa tatcctgtgt tatgttgttt attaataatc agaatcagcg
     5401 tcagtatcat accagtcaca acgccagaac gcccagcaca gccagatgac aggcgtagaa
     5461 taccgggaaa aaatccccgg gccagaaacg gggtaatgat tttcctgcac acgacaccag
     5521 accaacggtc agcaccgtca ttacaagacc ggccaccgcc gctgcatcac tggaggcaag
     5581 gttaagtgcc ggaataacgg ccagcaggca tgcaagcagc gccagacgtt ccgctctgtc
     5641 ttccgcccgg tacagtcggt aactgaccgc cagcatcagc agcccggcaa tgccgtaact
     5701 ggtgccggac aaaggccccc acagggccat cagaagaatg gccgctgctg tacgccaccc
     5761 actgcgcgtt tcacaccacg tcagcacctg tgccgccact gcaaaggcaa acaggatatt
     5821 cccctcatac cagggaaagc cggccagata atatgcgaac tgggcaataa ttccccatcc
     5881 ccacagccgg ttaatggccg gttgccggat atgcgcatga cgggacagat tcagccccca
     5941 caccagggca aacagcggaa aggctcctcg cccggccaga aacatccatt cctgcttcag
     6001 ctggaatatc agattgatgt ggtccagtac catcagcacc agcgccaccg ttttgatgat
     6061 atcccgctgt ccgggtgacc agaccagaaa cgactgcaac caggtatcgg tccgtgcagc
     6121 cagtgaatcg ttacgtgtcg tgttcgtgtt atccgttgtc atcagcgttt atcttcctgt
     6181 atcagtctcc gcccagggtt ttctctttct gcaggtcacg aaccatatcc cgctctatct
     6241 gctgcagacg ctcctgcagc agattttccc gggcaacctc ccggaccgct tcctgctccc
     6301 gttgtgattc acgcagcaca ctctccggca gcgcggcttc ccgttcagtt atgtcagccc
     6361 ggtcacgctc ctgcccggca atttccctga cagcctgctc tgttttgcca tccggcaggt
     6421 caggtttgtt ctctgccatt ttacggacaa tttcatctgc acggcgttcc gtttcacggc
     6481 ggatggcctc ttcagcctgc cgctgtgcca gcacctctgc cgtgaccggt tcgccatttc
     6541 cggctcccgg ctggacgctg ctgtccggga tatcccccca cacgcgacca ccggtgatgg
     6601 caccgggatt ccacggacga ccttcaccgg caattcttac caccacaccg ctgtcaggat
     6661 tatcacgggc aatccggaca ccatcctgca tattatcagc cagcaggctc tctccgttac
     6721 ggctaccctg cagggccacg aactgcgcat ccccgctgcc tttcacccgt ccttcaccgc
     6781 tgaatccccg cagcccgttt ccgtcatccg tggtcagtgg gttcagccag ataccggcgg
     6841 acttgccgtt acggtcaaac gccggcagtg ccacatacgg ctgcggatat ttacgtcccg
     6901 gtgcaataaa ccgtgcaggg ctgtctcccc cggccagccc tgcctgacgg agaacggcac
     6961 ggcctgccgc cacgtcccgc agttcccgtg ccgtactgaa cagccgctgc gcattcatga
     7021 cctcccggtc cggtttcggc tccagcacat cgtgggcagt tcctttctgt acggcattgt
     7081 taatggcatc cgtccagccc tgacggttat cggtgtacac ctgcacatgc tgcttcatac
     7141 gcgacagggc cacgtaggct gactcaaagc cggccatcag tttccggtta ccttccgtgc
     7201 cttcaagcgc gatggcaaag gtttcactcg ccccctgcgc accgtgggcg gtgatggcat
     7261 aagccaggtc aatatgttgc tctgcccgct cctggccggg gcgaatcacc cgggtctgct
     7321 gtccgtctga caacgtgaca ctgtcaccgg aaaccgctgt caccgtccag acgctgttgg
     7381 ccacataacc gcgctcccgg tcactcttcg tgaagcgcat ccggtcgccg gtccccaccc
     7441 ggattttgtc cggggtgtac agtgtgacac cttcagccac cgcctcccgg ggcgaaatca
     7501 gccgcgtgtt accttccgca tcctccaggg ttatcagccc gtcatccttg ctgataccgg
     7561 caatccggtg atacacacta tccaccaggg caagggcatc cgggttattc tcccaggtgg
     7621 agagacgacg cagctccccg tcacgtatat tcgctgtgtt caggacaggc accatgacct
     7681 gctctttccc cagctcaccg gccttttccc gtgcatcatg aatcatgctg ttcagtacgc
     7741 gccggtcctc attcaggtgc gtgacaatca gcgtctgctc ccgtgcttcc ggtgtcctgc
     7801 cggtatagtc gcggacaatg gcttcataca gtgtcatggg gacatccgga aaagtctcac
     7861 ctttcagcat cgccttctgc tgcgcttctg ccagtttcgc ttcctggctg tgactgaact
     7921 ccgtcacgga gtgctccggt gcccatgcgc cctcctgacg gggaacctga gacggtttca
     7981 cactctcaag cccggacagt gccctttcca catcccggtt aatcaggctg tataccgcct
     8041 cccgcagttc cggcgtctga cgcacaatct ccttcatgat gaccacatcg gcagcactgc
     8101 gcgtctgctg gagacggaaa ggctgaccgg gcgcgatggc ctgcagctgg tccgtgtcac
     8161 cactggccac ggcacgaccg ccaccggccg caatcagggc gtatgcccgt gccatgtcgg
     8221 tattgcccac cattgagctc tcatcgagca ggaacagcgt gttgctgaaa tccggcgttt
     8281 ctccgctgcg ctgctgcagc tgcgtgtcat gcagaaagga cgccagcgtc tgtgcatcca
     8341 cgccggcgct gcgcatctca ccgaccgcac ggtgcgtggg ccccagcccc acaactcggg
     8401 gacgctcact cgccggcagc atgttcacgg ctgacatcac cgcccggaac tgtgtggtct
     8461 tacccacacc ggcataaccc tgtaccaccg tgaaacggtc ggacgtttcc agtatcatac
     8521 gggtggcggc acgctgtccc gacgttaacg tctccatgag ttcgccgggt actctctcca
     8581 tcagcggcgt gaccgcctcc ttaccttcga gaatatggcg aagaatactt ttttctgcct
     8641 catacgacgc acgggaaacc agcaggcctg tgccatagcc ttttgccaca tccacataca
     8701 gtaagtcacc gcgttttatc tgcgcattga tttcccctcc cagttcagta aaaccggttc
     8761 cttctgcagc aaacgacttc gcctctgtca gcaggtccac catgctgaag gccaggtttt
     8821 tactttccag cacaggaagg gccagatgaa tggcctgctg tgccggcgtg tgcagcccgg
     8881 ttttctgcag actgatagcg gtttccagcg atgtctcacc ggcccgcgcc tttatctgct
     8941 cagaaaccac cgtaaaggag ggatggcggg caagtttttc cgcagtacgg gtttcatcca
     9001 gtgaggaata cagccggaca tcacgaccgc tgcgggccag accgttcagg gtggcgttgt
     9061 ccattgccat ctgtgtgacg gaggcaaaca ccgtcgcact gtcactgacg gaatgcccgg
     9121 gcgtttccac ccagccgttc tccagcttca gtgccgtgaa cggtgaatca gccacaggca
     9181 gggtggccgg ttcagcccgc cccggcacaa caaccgtcat cgcatcttca ctgacggatg
     9241 ccacctgcag gcggtcaccg ccggagacgc ggagtccggg aattttccct gtcaccctca
     9301 gtcgctcgcc gtctgccacc ggcatttttt ccggccggaa cagcgaccag ctgctgtcca
     9361 gggaggaaat acgcaccacc tgcgtttcgc cctgcgcatc ccgcagggtc aggctgtgac
     9421 tctgcgccgt cacccggtcg ataacatagc ggtcatgact gcgtgtctcc gggttccact
     9481 gctccatcac catccccggt cggtacatat cccgcagata acggctccgg ctgtccagcc
     9541 agaccggtga cagggcggtc atggtcacct ccgggtgtcc gagcacgccc tgtgttttca
     9601 gctcactgcg aatggcctgt gtcagtatgg cctgttcccg taccccgctg acctgtgcca
     9661 cgctctcttc tccggctttc acgctggccg caaaatctcc ggccagccgg tcatagcgga
     9721 cattccggtc cggttcactg atgatggttg ccggtcgctg ttctccgccc tgccagcgat
     9781 acgtgttcac cccggcatcc ttcatggcca tcagcgcact gcctgtaccg gttcgctgcc
     9841 cgctgtcggt tatcaggacc tgtacgttat gacgtgcggc accatccagc agggttaacg
     9901 tctctttcag ggagagtttt tcgccctggt cgacgataac agtactgccc ggcgtgaaga
     9961 ccatgccctc ctgcagctga cgacgtccgg ttatcagctc accggacagc cgttcatcct
    10021 gcttcaggtt catctgcgag cgacggtcag cagcgataat ctgcacctcc cgcccctgct
    10081 cccgggccat catgaccagt tcagccaccc gctcacgctg cccggctgca ccgccctgcc
    10141 cggacacaat ggccagcgac ggacggtcct gtgccagaac actgaccgca tcgctgtaac
    10201 cagccgtccg ggggacactt ttctccggat gtacggtcac ccggttctgt ttcatgatgt
    10261 cacgactgag tgcccggacg gacagctcat cgagcacatg aattcctgat gtgaacagcc
    10321 ccttctcacg gtcgaggggg ataagctgct cacggctgat ggcctcatcg ataccggcgc
    10381 gtgcccgttc aatcacacca ttttccggcg gcagtatgcc gaccgtcctg gccagcacgt
    10441 ccgtatacgt gaactgcact tttcgttcac ttaatccggc aatcgcctgt gtcaccgcct
    10501 gctgcacatc cggcccgtcc tgtgaagcag gcccgggcgc ctgcgtgcgg atctccgcac
    10561 gctgatccgc cgcgtcacga tatgcccgga tgtcgaaccc ggtttccttc agcgtctgca
    10621 tccattcagc cattctgact tcaggatcga cgtgctgttt ggattttcgc gtatccagtg
    10681 ccgccacatc ccgggatttc agcgaggcgt cttcaccgac ggcctccctg atagcctgtg
    10741 aacgactgga aaaggcctcc accggtacgc ccggcatctc ccacataccg tgcttaccga
    10801 ccacttcggt ttcataaccc agcgcctcaa cgcgctgacg taactcactc tgatatattt
    10861 tcccgaacgc aatccggttc gctagcacgt tctcggaaaa tccggttttc cccactttgt
    10921 cactgctcag tgtcttccac tcgccgttat gctgcgtgac attagccacc actacatgcg
    10981 tgtgtaactg tgggtcctga tcgcgactgg tgtcatggtt aaacagtgcc atcaccagat
    11041 taccggtcag caccgtttct gactgtccgt ccgtcatcac ccgtgtggaa gccagcgcct
    11101 ccacctgacg gacagcaaaa tccacggcct ggttatgtgc atcaataaga cgtttatccc
    11161 ccccgagcat ggccatcatg gagacacttt tgggggcgga gaaggtcagg tcgtagccgg
    11221 gacgatgctt attactgcca tcctgcatgc ggcttaaatc cgccccgtcc ggcagcctgc
    11281 cctccagaag acgggtaaaa acatccttat cgacgctgcc ctgcagccca agctgttcag
    11341 ccccctgacc agcccagcgt tctcccatgc tgcccagcac atagtaatta tccttgtcgg
    11401 tgtaatagtt tccggcactg cctgccgatt taacaacgct gaaactcaac atcagaaatc
    11461 atctcccggc tcaacatcct ccccgcgctc ccggtgcaca ttaatgttca cctcttcacg
    11521 acgctgcata tgctgctgga tgtccggatg attttcctgt tgccatgcct cataagcggc
    11581 catatccacc acttcaccgg attcactgat cccgggtggc agttgctgtt ccatctcttc
    11641 ttccggtttc attttcagct cctgctcgat cccccctgcc ggaacactca cacctgcatc
    11701 tgacttctta tcattgatga cagaagacac tggctgttgt ggctgttgtg gctgttgtgg
    11761 ctgttgtggc tgttgtggct gttgtggctg ttgtggctgt tgtggctgtt cagcctgagt
    11821 cacatcttct ccggaggcaa cctccggttc aaagaggctg gccatctgac gaccttctgc
    11881 ttcccttgcg gcaagtacgg cactcagacg gttctccatt tccgggttga tgtcacgcgg
    11941 aataaactcc ggagcgacct tcggtcgtgc ctgatatttc agagagagtt tgactgccgg
    12001 atacggtccg ggcagagtga catagcaggt cagatccggc agagactgaa tgtcggaata
    12061 actgaccagc gtctgacgct ccatatcctt accggtcgat accccgtcac gtaccgggtc
    12121 agcaccgtag gaatactgct cgctggcttt caggtgctct ttctcaccaa tctcaccggc
    12181 agcgaactct gcaatcttat ggctcgggga acggaaaaaa gcacgggtgt tcatgacgtc
    12241 aaacagcgtg gccgccgctt tctcaccgta gatatcttcc agctgggcat aggactggat
    12301 accaaacaca taacagccac cgaacttacg ggcttccggc aggatctcca ccagatccgg
    12361 cagtttgtgt aacgtgggta actcatcaca gaaaaaccac acacgacggt tacggttttc
    12421 tcccattgcc agcagaccac gaatggcaat ggacagccac atggagatca caggcttcag
    12481 ggaggcatgg gtgtcggcgt tcgacgaaat aaacagccag ccgtttttct gatcttcccg
    12541 gacaccacgc atccagtcac ggatggtgaa gggatcaccg ttatgctcaa tcccctgcag
    12601 gtaacggatg gctttcacgt agttggtcag cacagcacgg atggaaatcg ccgttttctc
    12661 aattttctct tccaccaggt tggccgccgg tgaattacgc aggaaggtac gcagtttttc
    12721 aattttgatg gaaagcagtg tgtccaccag tttgctgtag ctgcggttgg ggtcattacg
    12781 catcaggtac gccgcttccg caaaaatggt acgtcctgaa ccctgccaga acgggtcctc
    12841 ttttgtaccc atagggatca gcgtgttcgc ggtattatca aaatccggct gtgtcaggca
    12901 ctccttccac aaatcccatg cggcacagcg ggcatccagc ggattcagga ttttatcgat
    12961 ggagggatca tagtaacttt taacaaattc ccctgaacgg tcataaatca ccaccatatc
    13021 accgcgctga cgggcatagt tggccagacg acggataacc tccgacttac cggccccgac
    13081 cgtgccgtgc agacagaagt tctggatttc ggaatcccgg ataatcggca ggtcgccaat
    13141 ccggatatcg gagtccttgc cgtctttttt cagcatccgg gcaacgtctt tcggattgtc
    13201 tgtcagctga cgaccacctg tgacttcatt ttcgctctgt tgtttaccct gacgccccag
    13261 aatccaggag acaacaaaga aggtgatcag gcaaataacc agggccacga cagacgccag
    13321 aacgaatgca gaccatagct gctctccgca ccagatcata tatttatcat gcagtacctg
    13381 ggcagcgttc atccggaagg ttttgccgta atactggatc tcatagaccg gctgggattt
    13441 tattaaatcc cgcatgcctt ccagtgacgt acaccaccag taaatacagc cgttgataaa
    13501 cgtctgccag cttattttta cccataagac cagaccaatg aggatccaga aaaaaataaa
    13561 caggcagtaa agcatgatat tggcgatctg gctgaacatg cggatacgca tggacgcaat
    13621 ctgaccgccc tgggtcatat cctttgcgtt aaaactcatg atgaaactcc gggaaaatct
    13681 ggaacagggc agaaaaggat gatgtggggt taattcacag aaacagacca ggtgtaccct
    13741 gtatgccggt ggtgatgccc gcctgcagtc ctgccggaag agaccttctc tgtaacaatg
    13801 tcagtcaggc gcaatacttc agaccagtca ctcccatgcg gaaactggat ctgaataaaa
    13861 aggcgtaccc gttcacggtg atccagaact gtgtccggtt cataaaaacg gccactgtca
    13921 ggagaaacac aggacgtttt attgatgaga tcatacttat cggcaggaga ctgcgatgaa
    13981 atcgcccagg tgatattctc cgcctggtgg ccatcaatac gactgctccc cggcgtccat
    14041 gcaatttcct gcgcactcgc cacgatgatc tccctggaaa cttcagccag ctttctgggt
    14101 gtacagaata ccggtgccag cacacacagg gacaacaaat tacagctgac cgcctccggt
    14161 ttaagcaaat tgctcccgga tttttcagga aaagatattt ttgtcagtat ttcagttaat
    14221 tccttatgcc tgagagaatt aataaagtac atgataagca tcatgagaaa caatataaca
    14281 ataagccctc cgataaccac taaaaggtca gtatcaatat tattgctgta cagatagtct
    14341 ttagtggtca ctgtattatc ctgtatcacc ggggggtatt cagttacagg cttctcagta
    14401 aattgattat tctcaccaac atattcagcg atattgtctg atgtcataat gactttctcc
    14461 ggaagatccc ggcttcgccg gcaacacttc acggtcgcac aaagaatttg cacaaccgct
    14521 cagtgttttc atatttattt cggaaacgcc gggaggtgac aaaatttatt ttgttacatc
    14581 ccggggttgg acaacgagca acgcgaggcg tcagcaagga ggccggtcag atcagcctcc
    14641 tgacgaggtt cagagaatat ttgcgattga tttggccagt tggtcttcga gaacaggctt
    14701 cgcctcttca aatttcaggt taaccttgtt cgcatttgaa accacacggg tctggtattt
    14761 atgctggtta cctgtttcag tactggtctg aattttcgca cctgatgtgc cctgacgcag
    14821 ggcggcaaca ttatccgttg tcaccgttgc cttagtacgc tctgcaatct gtacatccgt
    14881 gatcatggta tagttcacat cttccaccat cgcatctgca gccataccca ccagaccagc
    14941 agcaaggcct acaccgagtg tggcaccggc agaatttgag ttatagccgg taataccggc
    15001 acctaacgct gcaccaactg ctgcgccttc ataaccacgg ttcagccatc cctgagactc
    15061 ccgcagatcc atcttatcgg ccttcagcac attcgcctga atccagtagt aggctttatc
    15121 cggagaagtc accacctgat atccttttgc tttcacagca tcagcaattt tgccctgcag
    15181 cccactcatg tctttatcag acgtgttttt gatctgcaga aataccgtgc gttcgctggc
    15241 gggttcaagc cagatggtct cactcatctg agtcttcacc tcaaggttac gcttcttgat
    15301 tgctgtgctc atcgcaccac accctgaaag ggccagagtg gaactgacca gtgcaaccat
    15361 catcaatttt ttcattttca tcaatgttcc ttcatattga ataaaacgtc ataatataca
    15421 aattaagatt cactcatcat atcgttttga actataacca tatgaattac caccgatatc
    15481 cacaccaata cctgacatag gtaaccctgt tgcaggatta gtacgatgaa aatccatatt
    15541 atcaaaatcc agatgcaaat cttcattagt ttgttcattt acctgagatt gcatccattc
    15601 tcttacgctt acacaaccac taactcgcct taattcgtat agtgtaccaa tgataaaaac
    15661 catcaggctt aataatggag agagtaagga agatactggc atcgttatat accaaacgcc
    15721 tttactaaac ttaaactcat ccgacacatt ccgttttttt cgtatttgag agaacaacca
    15781 tataaacagt ccctgccata gtactatgaa gaacagcata aaaattgttt cggcattccc
    15841 ccaactagcc agataacagg cgataaactg tatcaccgtt accagagtaa tatgggttaa
    15901 acatctcatt atcatctccc aaaaacagca ttattgctta tacttatcct gatattcctt
    15961 tgcccttttc atcagttcct gtggactgtc agccccgggc attctttcct gagccgactt
    16021 ttcttcatta tatttattat tctgagataa tgcctctgtt ttatggtgat tctgcagttc
    16081 agaatactgt cctcttacaa tattttcctc gccacgtacg ctgttctgag tatcacctat
    16141 attcccttta tattctgtga ccatattatc aacctgatgt tttacatcat tacggatatt
    16201 actgtcctgc gttctttgat caataatggc ctgatgcccc tgatggtcag caataatatc
    16261 ctgactgcca ccacccgaag gtacgctctc cattccttta ccaatatcac cacgggattc
    16321 ccgccaggca ttatcaacac caggctgtac ctgttcctgc acaaaagacc aggccatcgc
    16381 ccgacgacgt tctgcaatct ccggtgaact ggtgtttgtc agaatagctt ctgcatcctg
    16441 tggcgcgtgt ttcattacat actgtgcaaa ctgttgcgac aggtcttcac tcatctgccc
    16501 actcatgctt tcagtacggg atgccatttc agcatattca tggctgcggg tcatattcgt
    16561 cgtgtactgg tcgtaactct gcttcgctga attcagggct gcagacaact gatccacacg
    16621 ggaatcagca ttattgtccg tatggctgcc ggactcactg accttgcgac tggtaaaata
    16681 atcgctggcc tctttaaagt cttgtgtagc tctggcatcg atatcatgac gagcattatg
    16741 gctcgactga gtattactac ttgccgtatg cgcatcatta tcttcccaat caataccagc
    16801 ccgaccacca cctctaacgc ctaatcctcc accaacacca agaattttag gtttaacccc
    16861 ccattcagca tgagcatctc catacatccc ggcagaagct ctcgtacttc tggatgccag
    16921 ttcctgagtc gcctgctcat tactgatatt atgcgccttc gcatagcttt ccactgcact
    16981 gcgcatacgg ctggccatca tggagtcctg tgcgctcatc gtgctgtcag caccacctgt
    17041 gacagaatca ctgctccccc ggttagaacc aaactggctg agcgagttcc aggcactggc
    17101 aatactgctg ctgaacccat gcagggcgct ttctgctctg ttcgacgcct cccgggccat
    17161 ttcctgttgt gccgctgcaa tctgacgcgt tgcattgata ccgaccggta accgggacat
    17221 cgccccgctc gcatccatca ccatattacc gtcacgggtc tgtgtggcag ttgcgccact
    17281 gccggtctgg tacatcatct gaccaaacga cgtggtgctg ttggtgctcc agctgaagcc
    17341 gttcacgttt tccgtctgca tgttgccgta ggagtaatta ccgtcaacca cactacctgc
    17401 cgcactggca gtcgggctga tagcagagga agcgaaatgg ctgtacacgc tggaaaaacc
    17461 tgcgccaagg ccttttacca tcatccatga caacgggggt atcatgacag atatatatcc
    17521 ggcagtggac gccaggtcgg agtatttcag ctgtatctga gagagttcag agagcacgac
    17581 cggtgcacca ttctgctttg catagaatgt catggcactg ttcagaatgg catacagcaa
    17641 cggccaggtc tggaaccaca tcagagcaaa cacatagcct ctcagaacag acagcgtcag
    17701 tctgttgaag acagccgcca gtaccaggag cgggaagata ccaatggtaa tgcccaccag
    17761 aatggtctgc accatcggca ggtttcgcat cagcacatga ccaatggaga catgtgccag
    17821 acgctgtttc tccatcgacg aagtcgtggc cagactgacc aggctggcgg tatcaccgtt
    17881 acgggctgca ttactggtga taccctcttt cagggcattc atggtgacgt tctggcgcat
    17941 aatatgactg gcagactggc tggagccgta gaaataactg taactgtcac tcaccagttg
    18001 cctgaataac aggtccgggt ccggtctgcc gccaaatatc tgctgcacat aataatgcca
    18061 ggtcttgcct cccgtctttg tatcgagatt cagcctgtct ttcagcgtga ccgacgcatc
    18121 tttacaggtt atgaaattat tattgctgtc ataaacgccc cgtagcgggc tgggccggga
    18181 aaaaatcagc gtgtaggggt cagcggaggc catcaggtct tccagcgtgt atttgtggtt
    18241 caggtaaata tcccccagca cgcagttctg gacgtagtcc tgaaacagat tgatgatttc
    18301 cggattccgg gacagaaaat cggtgctttt cacaatcagg ttcgccccga acagcatgcc
    18361 tgtcttgctg taggtggcgc tgtcaggctg ggtgaatatc atctcgtaac tggccaccat
    18421 tgcatgcccg atacgggtcg tcagtgaaag tggcatcgcc agtccgacag gcacattatc
    18481 cacccggtga acctggacca ggtcactgtt atcaataatc tgcaccgatg tacggacatt
    18541 aaccagcagg ctgataagca caaacacggc cacccagccc agcaaatcca tcacgttgtg
    18601 ccgctgtacc cacatcacgg ccaccgcgag aacagacaat gtgagcgcaa ttttttcgat
    18661 ggaatcccac gtccgggtcc ccataaaggc ggcaatggcg ttcaggttat tgcgtaacca
    18721 ctcaccaccg gcaatcacat agacttcatt cacagcgtgc tccctccgaa gtgatagttg
    18781 ttctggtaac ggctgagcat gcgggcggaa agctgctggc gcatatagct catctgacgg
    18841 tcaacaacca gcaatgcatc ctgctggacc tttacctgcc cctggaaaac cgcaatctgt
    18901 tgctgtgcct gattcagatt ctccagcaca ctgtccagca cggtctgtgg atagttcccg
    18961 gtggcaatca tggcccgggc ctgctgaagc agttcctgaa tgtactggag catgatgtcg
    19021 tagccgatat agtccgtcag ctggtaaacc acgctgctgg atacaccgag catctgcggg
    19081 tcaataagat atttaaagac ggggacggtg gtgctggaaa tgaagccttt ttccctgtca
    19141 tccagtggag tatcactcac cgctttattc tgaatactgg tgagcagttt tgtaatctgt
    19201 gatttcaggg ctttatcccg ggcgatggtc acatttgcat ccgccaccac cttcagacac
    19261 ttgtccgaat cgttgcagtg ataaactttt gccgttcccc cctccataat cgcccggagg
    19321 atatcgcggt cagtggtacg cgccggcagg ggggtaatct cgctgtcttc cccaaaaatc
    19381 agcgtaccgg tgagcgtcat aataaattct ttcagttctt tgttaccatc aagcatcctg
    19441 tttttggaca gcgagtccca gatgatattg atgttcttca gcacccgttc cttatctttg
    19501 tcactggcct tgtcacgaac agacgatgac tgtcctccga ccgtgcagcc ctgacgggac
    19561 gcggcccagt cagcaaaaat attgctctca ccggcaatgt cctgacagac cttctgctgt
    19621 gacacctgag tctgaggaaa taatcctccg acaatcccct gcgccacctg acaggagctg
    19681 aggttcatac tgttaatgtc gcttgccatt ttctgcagga agtcttttgc ggttttgatt
    19741 tccggcaccg ttgcctgcag ggcaaggtca aaaaagtaac cggcagcgtt gctcataatc
    19801 tgcttaacaa aacgctgcaa ctgatcgcca ttaataaaac tgaacgagcc gagataggcg
    19861 tcgatgccgc cacatccggc attgatatcc ggcagcgtca tggaaatcag ctggatattt
    19921 ttgacctggg tgcgggcata cagggagcca ccataggcat acccggcggc ctgcccctgc
    19981 cagacgcccg gttgtgtggt attagaagca aatcccagct tattaaagaa ctgattcatg
    20041 tcgctgttca catccgctga cgctgcgggt gtgaccgtca gtaaagcggc acaaagaacc
    20101 agaagaggtt taatgcgaag cattattgcc accctccctt aatttcttct cccggcgcat
    20161 acggcgtttc agccggaata ttctgatccc cccgtttatt aattcattta aagggttcct
    20221 gacgtatacg ataagaaatc cacctgcaaa cgcccataac gtatacggaa tgacatacat
    20281 caggatgttc cacatataac gaaaattatt taattccggg tcagctttcc acgaatcaat
    20341 acaggcatca aaaatactat gcataatcac ccataaaaaa gttccggcag caacgaacac
    20401 aaaaaacaga tcaaaatata tatcagaaac cctgcgttca taatcataat cccgtttagt
    20461 ttttctgttt tttagttccg catcatttct tttttctttc attataagtt tcctctgata
    20521 tttccgatga ttcattcaga ccgttcatgc ggctgtaata tatttcagac accgaaatcc
    20581 atcccacaat tgcccccaca aaaaacacaa cagaaacaga aaaggtaaga cacagcgaaa
    20641 cgacaatttc cacagggctg gcgggataaa aaagcccctg acggataaag gggatattca
    20701 ggctgacgat tattcccgtc gcgatgccgg atattccggc aatgagtaac accacctgct
    20761 tatttcgtac ctttttcctc tccgtacatc tgcagaacgg tatccatccg cgccataaaa
    20821 ctggcggcat ccgttgcccc ctgcaaaagc ggtaatgcct caagtgtgtt gacgttgacc
    20881 aggaaggtag tcggtgtggc caccgggata ttcgggaaga aggtctgcat cacgtccggt
    20941 ggcaccggta atgcttccgg aaaggcggta tccccctgac catccagggt gtagggaaaa
    21001 acggaaaagc cgtactgctg tgccagctgt ttcagtaccg ggtcaaactg gtgacagtaa
    21061 gggcaatgcc cctgcataaa cagcaccacc ttccagtcgg ccaggttaac ctgcctgccg
    21121 ttgctgaggc ggaaccagcg gggagccggc ttttctgttc tggctgatgt gtctgctgcc
    21181 ggttgtgcag gctgcacggc cataccctgc ggattccaga gtcgttcaat ttcatcccgg
    21241 gtggaggcct gcacacctgt ggccatcatc aacaaaatga ccggcagtaa tttagtgaga
    21301 gacatgtccg ccctcctgct gcccttccat catggcctgt tgcctggctt ccagtgcctc
    21361 acgctcttcc aggctgacac gtgcaatcca tgcatccagc acctggtatg cgccgaacag
    21421 cacaaggatg gccgcaatca actccgccag aaaaaaagcc atccagggtt tgctgtaaac
    21481 aagacgggcg acgatatgaa accagacacc cagggaaaat acccacattc ccgtaatatc
    21541 aagccggggt aaagagaatc tgtatttact tatcatgtta aactccggtt attatttaca
    21601 tgagagtatt catgtctgta atattcgcgt ttgtgtgata ttaatcagat caacctcatt
    21661 attttgtatg gtttttatta ccgttttata tgacttagcc tgtcataagc ccccttataa
    21721 aaaaccacat cactccggac catgctgatg acaacataat acaaacaaac atccttctga
    21781 aactgtcaaa cttcgtgata accggtatgg tactgactgc cattgaaaca accggtacgg
    21841 cacactgatt cataaccgca ccacaaagaa acaagaaatt atctggcagc atacatacac
    21901 ttatgacatt tactacaaag aagacaaacg agctcagcag ataaactcct ttattatttg
    21961 gtcctgtaaa gaaataacca acaaccccca cgataaacag gctggcacct gtaaaaaaac
    22021 acaaatcccc tgtatgcata tataaccttt ggttaaaaat tcggtttaaa atcttcagaa
    22081 acgttcagga actgttttgc caggtcgtcc tgtgaaataa agccgtatga taacgggcga
    22141 acactgccct gtttcgggtc aaccagcatc atggccggga aatatttcac accgaggcgc
    22201 tgcgcctgcc cctggtcagt ccgggaatcc ggcaacagcg gattaatcac gccatccacg
    22261 gaaacgggaa taacactcag accataggta tccctgaagc cattaatgac ctgcgccagt
    22321 tgcccgtcga tggggtcctg cccccggtaa aagaacatga tgccgtagtg ttcagccagt
    22381 ttcgcaatgg cctgtcgctg ctgcgcctgg tctgccgcca gctggttccg taccgtgccg
    22441 ttgtaatggc tgtactgcag gttatagtcc agttcgggat gtgccagcat ggccttttta
    22501 gcactcatgg tgaaaagccc ggcctgctga gtccagtaat tctgcagccg gaaatattta
    22561 acaaaattat ccacgccggg atacagaatg gcttcgtaca gcgcccgctt tgttgccgtc
    22621 tgcagcgcgg ccagtttctg cataatatcc ggctcctgac ggggggctgc aggtacaggt
    22681 ttattttctt tttccttcgg atttattttc tcgttatacc actgccagcc tgcatctttc
    22741 cctgacgccg gaaaaataaa gcagcagagt aacagtggca gtaatgcttt attcatcttc
    22801 attcctttca tcattttttc tttcatcatc tttctgcagg ccagtcaggg cagaaaaaca
    22861 aaaatacaga ataaacagga gcacaccgga gtaaaggata acgaaagcag cggcaagcat
    22921 cccgtctgcc gtggtttctt ttaccgtatc ccacgaccag ataattaccg gggaaatgac
    22981 aatcgctgta atcgaaagcc ggataagaaa atcaacaaga cgattagaag tgaatataac
    23041 tttcatcatt tcccccggtt attttccata ccttactgtc ctgcctgttt cagctggtca
    23101 gcaatctgct ctttcacttt ctgcgtcaga acgccactgt ccgggatttt ctggttattc
    23161 atcaggtctt cgtagaagtt agtgaaatcc agtctgtcga actgaatttt ctgcagctca
    23221 tcaaccgtaa tcccccgaca gtcaggatgc ttcgcactgc caaacccgat acgcaactgc
    23281 ccgttacgcc cctgctgctg gactatctgc gccagcttcg aatcaaactg gcagtagctg
    23341 cgctttttct gcagacagac acccagcact ttcttcgaac agaactctcc gacactgacg
    23401 gtcagtttgt tgtcctttgc ctttcccagc gccttttctt cgctgttgca cttcgccaga
    23461 cccacatcct gaccccagcc accatcctta cagcagttac tgaagccggc tgcggcctta
    23521 cggcagaatt tcgcctcacc cgtgaaagcc ctgacatcca caccattcag ggcggcaaca
    23581 tccttacctg cggcggcaag cgcagccagc tgtgatactg cttcaccaaa atcactgctt
    23641 tttccgctct gtgccttatc gcattcaccg tcaaggcaga agacatcacc accacagacc
    23701 atcacttttc ctgatgttct ggactcgcag gagtacgtgg cgtactcatg cagacaggtt
    23761 ccctcatcgg aataaaacgc gcactgacgt gaggccagcg tacaggccgg attatcgaca
    23821 tatttctgac aggtgccgtt atctgcggac tgagtgacat atttatcccg gtatgcccag
    23881 catgcctgtg tgatgttcca gggtttacct tccatcactc cggttttcgt accacccggc
    23941 tctgaacact cagtgcctgt cagcacgcca tccgctttat taaacggaca actctccacc
    24001 cactccaccc ggggaaccca ttctttatca cggaccttca tcctgagttt cagcgtaaag
    24061 gtggaagcac cactgacaag cgattcatag actttcctgt caccattccc gtgcgggagg
    24121 caattaccgt tagcagtaca gccactaccg gtcagaacct gcccctgtgt cacctgcaaa
    24181 cctgtcgccc cggacagggg gtaagtatcc gactgcccgt ttgccaggcc gacattaaag
    24241 gttgtattca tgaaggtata acgggagttc aggaagaaaa aagcggcgta tacagacaat
    24301 gatgcacttt ccactgtacc ggttaccgga gccgtgacac tgaacaccag tttcagcccg
    24361 ttcatactga accggaactg tgagtgcggt atcgtcactt cacgatattc atccgtgtat
    24421 ttccagtccc cggtgatact ggcagtccgt gtgcagtact cctccaccat cgtgtcccgc
    24481 tcacaggtat agtttgtgaa ttcactgcgg tttatctcct gcgcactgca ctgctgtccg
    24541 gtatttccca caatgctgtc agcccggttc accacatccc tgccggtctg aatgaacggt
    24601 gcatccggtg acagaatgtc tttcggcttg ttcatgaagg actctgtgat ggtttttccg
    24661 gtttcaccgg tcgcccattc cgtggtgccg tcatttttca ggccgccatc cccgccggca
    24721 gtcacgccac cgtaatattt tgtctcgtcc ggattcgcgt tatagctggg gatactctcc
    24781 tgtggcttaa agccctgaat actgcctgta ccctgcccct gaatctgacg ggcaaaatca
    24841 gaaccggccc ggtaatcact gttgctgtca gcctgtgcca tgccggcaac cagagccagt
    24901 atcagaggta aaatacgttt catttcccgg aatctccttt ccctgccagt aaatcatgag
    24961 ccacctgccg gcagtcaccc gtggcggcca ctttctccag cgcctggccg acccgcaggt
    25021 ttccccggat gatgtcgtat ccctgactgc agaacaccac cagtgccggt acactgcgaa
    25081 tgccgtactg tgaaaacagc gtcgggtcga tctgaacacc atcagtcgcg ccgtctttca
    25141 ccagggacag cacggcttca gcggtggtct tcaggtcatt gttcaccatg ccccgcagtg
    25201 tggcgggtat accgtagtgc cgggtttcgc ccagcatccg tttcagcccc tcttcgggaa
    25261 tggaaaacga cacaaaatac agggcaccct gccggggttt cccgtcctgc gaagcctgct
    25321 gtttacgaac cagctcatcc agaaaatggt tatctgaacg ctgaaggggg ttttccagca
    25381 cctgtttctc cgcccaggct ttcagctgat ggtcaggttt ctctcgcagt tgtctgctta
    25441 aattttcctg ctgcttcagg aactggcggt tttcagttgt gttcacattt tctgaagcat
    25501 gaacagcaag atgcatccct gctgtcagag caaaaagtgc aaatattttc cggttcatga
    25561 tatttctcac tttctgctat ttacaggcat gaactcatca gaaaaaagat aattgagaaa
    25621 aacagccaca aacgcacatg taaatgtcac cttcagatat aatctgacaa gtgtcacttg
    25681 ttctgatata tctgcgttct tccattcatg aataatttca gcgagaaaac tgtcaggtgc
    25741 aaaataaaat aatataacgg gcactattac aagaccaaaa ggaaataatt ttaatgttct
    25801 gcttatcagc ctgagcagta cagcacttat tttcctgagg tgctgaatat atttactgtc
    25861 cattgttgcc attttcattt cctcttattc attaaaatga tttacaggaa tacacagtta
    25921 cgtttacgcc acatcaggta gccgaagttt ttctttgtgt tgggcggatt ttttcccgtc
    25981 tcccagcgca tcacactgcg cccgaacgga tggcactgtc cgctgtccgg atacatattc
    26041 accatctgat aacgccagcg ttctttaggc aggattgggg acggatattc attacagacg
    26101 gcgttatttt tcccgatggt ttccataatc atgccctgac ggtgtagttt gaacgccatg
    26161 cgttcactga ccagcaggga ggactgcaac ggactggact cattactcac ccagccattg
    26221 aacgggtaca tactgccctg cgaaccggca caccagaaca gaacatcgag aggcatatta
    26281 aaggcgctgg caatggcatc tgctgcacag gccccctgtg ctatcggatt ggcaaagatg
    26341 acagcttccg gattgagaat ggtggtcagg ctgctgtccg tccaggtggg gtcaatttca
    26401 gaaagataag cgatatccag gtcaccacct tccagacagc ccagcgatgt gatgatgtta
    26461 agccagtacg tcagcgggta tttgtaccag tgaacgtgat agaacgcccc gttcacctgc
    26521 ttctcatcct ttttcgccgt tccctgtgcc gttttaccaa aagccggcag gctgaagccc
    26581 aggttcacca tgcatcccgg tgaccgggtg acgtccgtca gcgccatcgg ctcccagtaa
    26641 ccaatggcca gcccgatacg cctgaacagc ggcggcggtg ccggacaaat ctgaatgggc
    26701 atcgacgggt tcgccgtgtc ggggacctta ccctgactga ctttgatact gcccagcgag
    26761 agcgggaaaa tacagctcca gcagatatcc gtgatcgggt ttacaaaacg cccctcacag
    26821 gcagaatccg cagatgccgc agggacatga ccggcgagaa ggaataacat cagcagccac
    26881 aggcttcgct tcattttctg ccctcctctg ccggaataaa ttccaccttc aggaagcgat
    26941 cgccgggaac ggcgctcacc cgtgccggga cctgatcaat gccgaggcgc tggcagagca
    27001 caccgttctg gtcaaagtaa acacggctgt ccagagactt ctgcatctcc gggatactgc
    27061 cctgcacgag gataattttg ctctccagtg tgggcggcgt ctggcgtttc atccaggcca
    27121 cctgcgccgg atcatcgcca ttgatgaaat acagcgtctg gttaaaaggc acatactgca
    27181 gcggattcat cacttcaccc tgacgggcaa acacccgccc ttcgttatcc cggatatccg
    27241 cagccagtct gacggacggg tcaaacaacc ggctgctgta tttctccgtg cgtccgatac
    27301 cgggcacggc agggggccgc agactgttgc ggatcacccg ctccttaaag gcatccattt
    27361 tccggcccat ctcaccggac tgctccagtg ccgtcaggcg ctgcatgatc accgtcagca
    27421 tgtccggctc ctttaccggc cacagatcgc cccaggtgcc aagatcggcg gcggccacac
    27481 tctgccccca tatcagcagg gcaatcagcc cccggcatct catggttccg ctctcattcg
    27541 ccgggcaata tcctgctgga tttcccgggt gatatccgga gccccctgta ccacggcagg
    27601 cgacaccagg atgaccgcgt ggtgtttctg ctgccacgcc tgcaggctgg cttccagtgc
    27661 cgtgttaaaa cgcgctgaaa gggcttttga ctgtgcttct gacagttgtt tctgactggc
    27721 gctgtcaaaa aacgcatcca cggtctgttt catgttgaag gcggcagtca ccggtgcgtt
    27781 cagtctgaca atgccgtaac tgacggctgc gttcagtaac accattgcca ggtatcccgg
    27841 caccgtccac caccagtggc tgcgtcgggg ggctgtaacg tcagtcgttt tttgcgttat
    27901 ggtcatgcaa cactcctgta tttctcatgt tcttccagcc aggcttccag cgaagccatc
    27961 tccggccctg acttcttcca cgccagctgc cacaccgcct catgaatact cagcccctct
    28021 ttacgtttct gctgcacaaa ctcaaaatcc gggccgtcag agctgtacat ggcgcggctt
    28081 aacgggtcca caaacaggcg gtgccaggag gaatggtttt ccacctgcag caggaaggaa
    28141 ctgaaccact ggtctttggc cgcaccaaac ttaccaatca tgtcgcgctg cagtggctgg
    28201 aactggtccg gatacagctg gttgtatttc gcgaactcct tcgcactctg tctgaggata
    28261 attttgtagg aggagttacc ccatgccgcg cgggcagcac tggaggcctt gtcagagtca
    28321 aaatcgacga tgttctgtgt gatggtgata taggcaccgg tatgacggcg ggccgtacgg
    28381 tagcctttct caataaattc accgaccttg tgatttttga agtccagcag acgccagcct
    28441 tcatcaataa cgttcagttt cttgaggtta cgcggcgtgc ggtacatcct gttctcgatg
    28501 tagataatca gggagaacat caccgcaacc agcagtgacg gacgatcttc cagtccgccc
    28561 agctccagca ccaccatttt tgcgtcatcc cgcagggacg gctcatcaga gttaaaatac
    28621 tggccgtaag tgccgttggc agtgtactgg tcaagcagca caatcatttc gtccagacgg
    28681 ctgcggatgg tcggtgaccc ggcatactgc tcgctgtcac tggcgttttt caggaaatcc
    28741 accacgtcat caatacgtgc cctgttctct ttggccagcc aggaggccct gaccgcctgc
    28801 agcagcagac cttcatgcac ttcatccagg ttaccgttgg ggctggccat cactgacaac
    28861 tggtcacgga cacgctccgc tgactggtca atatcggtga tgttcgcaaa cgggttaaaa
    28921 cgcagggttt caccgtccag atatacgcca cccatgttct cacacaggga cttgtagcca
    28981 tcccccatgt cgaacaccac ggcaaagccc ccggagtcca gcacgctgcg gataagtggc
    29041 tgtatcagcc cggttttacc ggccccggag gtgccacaga ccgccatgtt gtagttggtg
    29101 ttattcatcc cccggaagaa aatatcgata aacgccagct ggttacggta ggtgggtgcc
    29161 agcagacctg ccggtgtcag ggggttatcc gccactaacg gcatcaggtt ggcaacatta
    29221 aagctctctg cgcgctgaac cactccggcc tctttcagct gtttaaacaa ccctttcccg
    29281 gccataaagg gcagacaggt caggaaattg cgcatgtggt taaagcgcgg tgaaatcagc
    29341 tcaaaaccgt ttttacggaa gctgttcagg atgtcctgct ccacttccag cgccgtttca
    29401 ttgttgtcct tgcagaatgc tgtgatgttg aggaagtagg acacgacaga agactgaccg
    29461 gagcccagac gctgacgcag ttccccccac tccttcgcct ctttctccac ggacggaaac
    29521 catttggcat aggaggtctt cgactttttc tccaggtcca tgtacttcag gttggcttcg
    29581 ctgtgggttt tcacctggtc ttccaccacc agagtcagcg tcaggatgaa cggacaggag
    29641 atggacagtt ccgggttcag caggttgctg tagttgtcgg ccatattcca caggaaggcg
    29701 atttccgggt tacgggccag atggaaattc aggatacggg ccgtgctgtt cctgccgttc
    29761 tcacgcaggc ccagcgtcag gtaatcagcc cggacattca ggtcaaaact gtcctccaca
    29821 cactgataat tcagatcaga atacgggtcc agctgacgtc ttttcgggta cagggagtcc
    29881 gggttatggt taatcatctc cccgacaata tcgataaagg cctgtgcatc caccgtctgt
    29941 gtggcgatac tggccccctg taacgacgcc cggatgattt tcaccaggtt ttccatttcc
    30001 agaatgtcag cccggctttt tttcttcgag ggagaacagt acgagataaa tacccggtaa
    30061 tggcgcaggg tcaggggcag attcatcccc tccggcagcg gaaactgtgt cgctgccgct
    30121 ttcatataat aggcccgggt aatggcgtta aaccgttcag cctgttcacc tgaccaggag
    30181 aactcacgca gcccgtactc aatcctgtca ccaaccagct gactggacat cagatggata
    30241 cacaacggaa taccgcgcgg cagtttggtg cgcagcatgt gatcgagtgc ctccacaatg
    30301 gacttattcg ccccattgat gggaatggct tccagcataa agcccatcgt ggtgtcattc
    30361 atgaacagac cggattcctg gttgtaatca cggtacggca gcagacggct gaactgcggg
    30421 aagctcattt cgcccagaac ttcattggcc tttgcggatt cgtcaggcag tttcagtgct
    30481 gtgacgaggg agttaaccgc ctgagtgacg gcctcaagtg ggttattact catatgagta
    30541 ttccttagtg cgtttaaaaa aggaaaatat cagtctgtgg ccgtggatat atcacgcagt
    30601 gccattcctg aaaccacagc acaaataaga gatggaggct tttaacagga aaccaggatt
    30661 aacgttcctt tactgaatga gcatcattta atgcctgaat ttgtttttcg cgaatcttat
    30721 tgcgctcatt ttctcttaca cgtttcacct ctgccatttc gtttcttttt ctgcattctg
    30781 atgcgatttt atttgcctta ccaagtgtta aaggttcaga ctgataatta agtgtctcct
    30841 gaaatgcttc tgctgcacag ggagtttcct gtaaaatctc tgtcagttgt tctggtgttg
    30901 caggttgttg cgcaaggtaa acgatgtaaa aaggaataca tacaatcata gccataacgc
    30961 caacaaatat aaaagccaga acagatttta aatatatcga cttcatttca ttttccttcg
    31021 aagcagtaag cactcattgc ccaccaccat ttagtgtttc catcacgctg ttcaaaggtt
    31081 catttgtttt aacttcaata cactcccctg tatttccaaa gcccctgccg ccatgtgctt
    31141 cgattgtccg aagaacctgc acgcatacgt ccgtatttgg catgggtgta atattccagc
    31201 ttccgccacc gttaatattt tttatcagca caagaaaaat aaaggtatgc attctttatt
    31261 atctccgctt gtatattaat cactgaatat cccttcagtt aacttatgag gtcataaaac
    31321 actgcagatt ctcattcgga tttattccct tcagtaaact tccgaatagt atcctgttca
    31381 cgctgacggg tggcagactc ctgcattgca tccacttt
//
LOCUS       DAZPEU010000027        30219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPEU010000027 DAZPEU010000000
VERSION     DAZPEU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.027"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..225
                     /gene="tuf"
                     /locus_tag="ACHU2L_001164"
     CDS             <1..225
                     /gene="tuf"
                     /locus_tag="ACHU2L_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR1356109.1"
                     /translation="TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLI
                     HPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            455..838
                     /gene="secE"
                     /locus_tag="ACHU2L_001165"
     CDS             455..838
                     /gene="secE"
                     /locus_tag="ACHU2L_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR1356110.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            840..1385
                     /gene="nusG"
                     /locus_tag="ACHU2L_001166"
     CDS             840..1385
                     /gene="nusG"
                     /locus_tag="ACHU2L_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR1356111.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1544..1972
                     /gene="rplK"
                     /locus_tag="ACHU2L_001167"
     CDS             1544..1972
                     /gene="rplK"
                     /locus_tag="ACHU2L_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR1356112.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1976..2680
                     /gene="rplA"
                     /locus_tag="ACHU2L_001168"
     CDS             1976..2680
                     /gene="rplA"
                     /locus_tag="ACHU2L_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR1356113.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3093..3590
                     /gene="rplJ"
                     /locus_tag="ACHU2L_001169"
     CDS             3093..3590
                     /gene="rplJ"
                     /locus_tag="ACHU2L_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR1356114.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3657..4022
                     /gene="rplL"
                     /locus_tag="ACHU2L_001170"
     CDS             3657..4022
                     /gene="rplL"
                     /locus_tag="ACHU2L_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR1356115.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4342..8370
                     /gene="rpoB"
                     /locus_tag="ACHU2L_001171"
     CDS             4342..8370
                     /gene="rpoB"
                     /locus_tag="ACHU2L_001171"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR1356116.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8447..12670
                     /gene="rpoC"
                     /locus_tag="ACHU2L_001172"
     CDS             8447..12670
                     /gene="rpoC"
                     /locus_tag="ACHU2L_001172"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR1356117.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12912..14045)
                     /gene="thiH"
                     /locus_tag="ACHU2L_001173"
     CDS             complement(12912..14045)
                     /gene="thiH"
                     /locus_tag="ACHU2L_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR1356118.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14042..14812)
                     /gene="thiG"
                     /locus_tag="ACHU2L_001174"
     CDS             complement(14042..14812)
                     /gene="thiG"
                     /locus_tag="ACHU2L_001174"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR1356119.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14814..15014)
                     /gene="thiS"
                     /locus_tag="ACHU2L_001175"
     CDS             complement(14814..15014)
                     /gene="thiS"
                     /locus_tag="ACHU2L_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR1356120.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14998..15753)
                     /gene="thiF"
                     /locus_tag="ACHU2L_001176"
     CDS             complement(14998..15753)
                     /gene="thiF"
                     /locus_tag="ACHU2L_001176"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR1356121.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15746..16381)
                     /gene="thiE"
                     /locus_tag="ACHU2L_001177"
     CDS             complement(15746..16381)
                     /gene="thiE"
                     /locus_tag="ACHU2L_001177"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR1356122.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16381..18276)
                     /gene="thiC"
                     /locus_tag="ACHU2L_001178"
     CDS             complement(16381..18276)
                     /gene="thiC"
                     /locus_tag="ACHU2L_001178"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR1356123.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18357..18457)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18510..18986)
                     /gene="rsd"
                     /locus_tag="ACHU2L_001179"
     CDS             complement(18510..18986)
                     /gene="rsd"
                     /locus_tag="ACHU2L_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR1356124.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19081..19854
                     /gene="nudC"
                     /locus_tag="ACHU2L_001180"
     CDS             19081..19854
                     /gene="nudC"
                     /locus_tag="ACHU2L_001180"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR1356125.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19894..20958
                     /gene="hemE"
                     /locus_tag="ACHU2L_001181"
     CDS             19894..20958
                     /gene="hemE"
                     /locus_tag="ACHU2L_001181"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR1356126.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21115..21786
                     /gene="nfi"
                     /locus_tag="ACHU2L_001182"
     CDS             21115..21786
                     /gene="nfi"
                     /locus_tag="ACHU2L_001182"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR1356127.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21829..22419
                     /locus_tag="ACHU2L_001183"
     CDS             21829..22419
                     /locus_tag="ACHU2L_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR1356128.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22606..22878
                     /gene="hupA"
                     /locus_tag="ACHU2L_001184"
     CDS             22606..22878
                     /gene="hupA"
                     /locus_tag="ACHU2L_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR1356129.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22891..23586
                     /locus_tag="ACHU2L_001185"
     CDS             22891..23586
                     /locus_tag="ACHU2L_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR1356130.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23588..24013)
                     /gene="zraP"
                     /locus_tag="ACHU2L_001186"
     CDS             complement(23588..24013)
                     /gene="zraP"
                     /locus_tag="ACHU2L_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR1356131.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24251..25627
                     /gene="zraS"
                     /locus_tag="ACHU2L_001187"
     CDS             24251..25627
                     /gene="zraS"
                     /locus_tag="ACHU2L_001187"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR1356132.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25624..26949
                     /gene="zraR"
                     /locus_tag="ACHU2L_001188"
     CDS             25624..26949
                     /gene="zraR"
                     /locus_tag="ACHU2L_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR1356133.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26946..28235)
                     /gene="purD"
                     /locus_tag="ACHU2L_001189"
     CDS             complement(26946..28235)
                     /gene="purD"
                     /locus_tag="ACHU2L_001189"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR1356134.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28247..29836)
                     /gene="purH"
                     /locus_tag="ACHU2L_001190"
     CDS             complement(28247..29836)
                     /gene="purH"
                     /locus_tag="ACHU2L_001190"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR1356135.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7071 a         7881 c         8314 g         6953 t
ORIGIN      
        1 actccgttct tcaaaggcta ccgtccgcag ttctacttcc gtactactga cgtgactggt
       61 accatcgaac tgccggaagg cgtagagatg gtaatgccgg gcgacaacat caaaatggtt
      121 gttaccctga tccacccgat cgcgatggac gacggtctgc gtttcgcaat ccgtgaaggc
      181 ggccgtaccg ttggcgcggg cgttgtagca aaagttctga gctaattgcc gataacattt
      241 gacgcaatgc gcactaaaag ggcatcattt gatgcccttt ttgcacgctt tcgtaccaga
      301 acctggctca tcagtgattt tctttgtcat aatcattgct gagacaggct ctgttgaggg
      361 cgtataatcc gaaaagctaa tacgcgtttc gatttggttt gcctcgcgat cgcggggtga
      421 aaatgtttgt agaaaacttc tgacaggttg gtttatgagt gcgaataccg aagctcaagg
      481 aagcgggcgc ggcctggaag cgatgaagtg ggtcgttgtg gtggcattgc tcctggcggc
      541 gattgtcggc aactaccttt atcgcgacat tatgctgccg ctgcgtgcgc tggccgtagt
      601 aattctgatt gctgcagcgg gtggtgtcgc gctgttaacg acaaaaggta aagctaccat
      661 tgcttttgcc cgtgaagcgc gtaccgaagt ccgtaaggtc atttggccga ctcgccagga
      721 aacattgcac accacgctga ttgtggctgc ggttaccgca gtaatgtcac tgatcctgtg
      781 gggactggat ggtattctgg ttcgcctggt atcctttatc actggcctga ggttctgaga
      841 tgtctgaagc tcctaaaaag cgctggtacg tcgttcaggc gttttccggt tttgaaggcc
      901 gcgtagcaac gtcgctgcgt gagcatatca aattacacaa catggaagat ttgtttggtg
      961 aagtcatggt accaaccgaa gaagtggttg aaatccgtgg cggtcagcgt cgcaaaagcg
     1021 aacgtaaatt cttccctggc tacgtcctcg ttcagatggt gatgaacgac gcgagctggc
     1081 acttggtgcg cagcgtaccg cgtgtgatgg gcttcatcgg cggtacttcc gatcgtcctg
     1141 cgccaatcag cgataaagaa gtcgatgcga ttatgaaccg cctgcagcag gttggtgata
     1201 agccgcgtcc gaaaacgctg tttgaaccgg gtgaaatggt ccgtgttaat gatggtccgt
     1261 tcgctgactt caacggtgtt gttgaagaag tggattacga gaaatctcgt ctgaaagtgt
     1321 ctgtttctat cttcggtcgt gcgaccccgg tagagctgga cttcagccag gttgaaaaag
     1381 cctaacccag cgatcaaaaa agcggcgatt taatcgttgc acaaggcgtg agattggaat
     1441 acaatttcgc gccttttgtt tttatgggcc ttgcccgtaa aacgattttt tatatcacgg
     1501 ggagcctctc agaggcgtta ttacccaact tgaggaattt ataatggcta agaaagtaca
     1561 agcctatgtc aagctgcagg ttgcagctgg tatggctaac ccgagtccgc cagtaggtcc
     1621 ggctctgggt cagcagggcg taaacatcat ggaattctgc aaagcgttca acgcaaaaac
     1681 tgattccatc gaaaaaggtc tgccgattcc ggtagtaatc accgtttacg ctgaccgttc
     1741 tttcactttc gttaccaaga ccccgccggc agcagttctg ctgaaaaaag cggcaggtat
     1801 caagtctggt tccggtaagc cgaacaaaga caaagtgggt aaaatttccc gcgctcagct
     1861 gcaggaaatc gcgcagacca aagctgccga catgactggt gccgacattg aagcgatgac
     1921 tcgctccatc gaaggtactg cacgttccat gggcctggta gtggaggact aagaaatggc
     1981 taaactgacc aagcgcatgc gtgttatccg tgagaaagtt gatgcaacca aacagtacga
     2041 catcaacgaa gctatcgcac tgctgaaaga gctggcgact gctaaattcg tagaaagcgt
     2101 ggacgtagct gttaacctcg gcatcgacgc tcgtaaatct gaccagaacg tacgtggtgc
     2161 aactgtactg ccgcacggta ctggccgttc cgttcgcgta gccgtattta cccaaggtgc
     2221 aaacgctgaa gctgctaaag ctgcaggcgc agaactggta ggtatggaag atctggctga
     2281 ccagatcaag aaaggcgaaa tgaactttga cgttgttatt gcttctccgg atgcaatgcg
     2341 cgttgttggc cagctgggcc aggttctggg tccgcgtggc ctgatgccaa acccgaaagt
     2401 gggtactgta acaccgaacg ttgctgaagc ggttaaaaac gctaaagctg gccaggttcg
     2461 ttaccgtaac gacaaaaacg gcatcatcca caccaccatc ggtaaagtgg actttgacgc
     2521 tgacaaactg aaagaaaacc tggaagctct gctggttgcg ctgaaaaaag caaaaccgac
     2581 tcaggcgaaa ggcgtgtaca tcaagaaagt tagcatctcc accaccatgg gtgcaggtgt
     2641 tgcagttgac caggctggcc tgagcgcttc tgtaaactaa tgcctttacg tgggcggtga
     2701 ttttgtctac aatcttaccc ccacgtataa tgcttaatgc agacgtatat ccgagatatt
     2761 cgggttgtgg caaggcggca actgagtgag tcgccaagag catagctaac tatgtgactg
     2821 gtgcgaatga aggaagccaa cgccgccaca agttgaatag cgacggatag aaaagatttg
     2881 ttcgttggag cctggcctat ccaggcctcc gtcgaagacc gcaggagttt cgcaagaaac
     2941 ttaatcccct gcgtagacgg tgacagaacg ctaagattat tcttttatat tctggcttgt
     3001 ttctgctcac cgtaattaag acgctctctc cgtttggagg agtgaagtga gttccagaga
     3061 ttttctctgg caaacatcca ggagcaaagc taatggcttt aaatcttcaa gacaaacaag
     3121 cgattgttgc tgaagtcagc gaagtagcca aaggcgcgct gtctgcagta gttgcggatt
     3181 cccgtggcgt aactgtagat aaaatgactg aactgcgtaa agcaggtcgc gaagctggcg
     3241 tatacatgcg tgttgttcgt aacaccctgc tgcgccgtgc tgttgaaggt actccgttcg
     3301 agtgcctgaa agacgcgttt gttggtccga ccctgattgc atactctatg gaacacccgg
     3361 gcgctgctgc tcgtctgttc aaagagttcg cgaaagcgaa tgcaaaattt gaggtcaaag
     3421 ccgctgcctt tgaaggtgag ctgatcccgg cgtctcagat cgaccgcctg gcaactctgc
     3481 cgacctacga agaagcaatt gcacgcctga tggcaaccat gaaagaagct tcggctggca
     3541 aactggttcg tactctggct gctgtacgcg atgcgaaaga agctgcttaa tcgcagttat
     3601 ctttttaacg cattcgctta cgtataaact tattctgata ttcaggaaca atttaaatgt
     3661 ctatcactaa agatcaaatc attgaagcag ttgcagctat gtctgtaatg gacgttgtag
     3721 aactgatctc tgcaatggaa gaaaaattcg gtgtttccgc tgctgctgct gtagctgtag
     3781 ctgctggccc ggttgaagct gctgaagaaa aaactgaatt cgacgtaatt ctgaaagctg
     3841 ctggcgctaa caaagttgct gttatcaaag cagtacgtgg cgcaactggc ctgggtctga
     3901 aagaagctaa agacctggta gaatctgcac cggctgctct gaaagaaggc gtgagcaaag
     3961 acgacgcaga agcactgaaa aaagctctgg aagaagctgg cgctgaagtt gaagttaaat
     4021 aagccaaccc ttccggttgc agcctgagaa atcaggctga tggctggtga ctttttagtc
     4081 accagccttt ttgcgctgta aggcgccagt agcgtttcac actgtttgac tactgctgtg
     4141 cctttcaatg cttgtttcta tcgacgactt aatatactgc gacaggacgt ccgttctgtg
     4201 taaatcgcaa tgaaatggtt taagcgtgat agcaacaggc attgcggaaa gtgttccatt
     4261 ttccggtcaa caaaatagtg ttgcacaaac tgtccgctca atggacagat gggtcgactt
     4321 gtcagcgagc tgaggaaccc tatggtttac tcctataccg agaaaaaacg tattcgtaag
     4381 gattttggta aacgtccaca agttctggat gtaccttatc tcctttctat ccagcttgac
     4441 tcgtttcaga aatttatcga gcaagatcct gaagggcagt atggtctgga agctgctttc
     4501 cgttccgtat tcccgattca gagctacagc ggtaattccg agctgcaata cgtcagctac
     4561 cgccttggcg aaccggtgtt tgacgtccag gaatgtcaaa tccgtggcgt gacctattcc
     4621 gcaccgctgc gcgttaaact gcgtctggtg atctatgagc gcgaagcgcc ggaaggcacc
     4681 gtaaaagaca ttaaagaaca agaagtctac atgggcgaaa ttccgctcat gacagacaac
     4741 ggtacctttg ttatcaacgg tactgagcgt gttatcgttt cccagctgca ccgtagtccg
     4801 ggcgtcttct ttgactccga caaaggtaaa acccactctt cgggtaaagt gctgtataac
     4861 gcgcgcatca tcccttaccg tggttcctgg ctggacttcg aattcgatcc gaaggacaac
     4921 ctgttcgtac gtatcgaccg tcgccgtaaa ctgcctgcga ccatcattct gcgcgccctg
     4981 aactacacca cagagcagat cctcgacctg ttctttgaaa aagttatctt tgaaatccgt
     5041 gataacaagc tgcagatgga actggtgccg gaacgcctgc gtggtgaaac cgcatctttt
     5101 gacatcgaag ctaacggtaa agtgtacgta gaaaaaggcc gccgtatcac tgcgcgccac
     5161 attcgccagc tggaaaaaga cgacgtcaaa ctgattgaag tcccggttga gtacatcgca
     5221 ggtaaagtgg ttgctaaaga ctatattgat gagtctaccg gcgagctgat ctgcgcagcg
     5281 aacatggagc tgagcctgga tctgctggct aagctgagcc agtctggtca caagcgtatc
     5341 gaaacgctgt tcaccaacga tctggatcac ggcccatata tctctgaaac cttacgtgtc
     5401 gacccaacta acgaccgtct gagcgcactg gtagaaatct accgcatgat gcgccctggc
     5461 gagccgccga ctcgtgaagc agctgaaagc ctgttcgaga acctgttctt ctccgaagac
     5521 cgttatgact tgtctgcggt tggtcgtatg aagttcaacc gttctctgct gcgcgaagaa
     5581 atcgaaggtt ccggtattct gagcaaagac gacatcattg atgttatgaa aaagctcatc
     5641 gatatccgta acggtaaagg cgaagtcgat gatatcgacc acctcggcaa ccgtcgtatc
     5701 cgttccgttg gcgaaatggc ggaaaaccag ttccgcgttg gcctggtacg tgtagagcgt
     5761 gcggtgaaag agcgtctgtc tctgggcgat ctggataccc tgatgcctca ggatatgatc
     5821 aacgccaagc cgatttccgc agcagtgaaa gagttcttcg gttccagcca gctgtctcag
     5881 tttatggacc agaacaaccc gctgtctgag attacgcaca aacgtcgtat ctccgcactc
     5941 ggcccaggcg gtctgacccg tgaacgtgca ggcttcgaag ttcgagacgt acacccgact
     6001 cactacggtc gcgtatgtcc aatcgaaacc cctgaaggtc cgaacatcgg tctgatcaac
     6061 tctctgtccg tgtacgcaca gactaacgaa tacggcttcc ttgagactcc gtatcgtaaa
     6121 gtgaccgacg gtgttgtaac tgacgaaatt cactacctgt ctgctatcga agaaggcaac
     6181 tacgttatcg cccaggcgaa ctccaacctg gatgaagaag gccacttcgt agaagacctg
     6241 gtaacttgcc gtagcaaagg cgaatccagc ttgttcagcc gcgaccaggt tgactacatg
     6301 gacgtatcca cccagcaggt ggtatccgtc ggtgcgtccc tgatcccgtt cctggaacac
     6361 gatgacgcca accgtgcatt gatgggtgcg aatatgcaac gtcaggccgt tccgactctg
     6421 cgcgctgata agccgctggt tggtactggt atggaacgtg ctgttgccgt tgactccggt
     6481 gtaactgcgg tagctaaacg tggtggtgtc gttcagtacg tggatgcttc ccgtatcgtt
     6541 atcaaagtta acgaagacga gatgtatccg ggtgaagcag gtatcgacat ctacaacctg
     6601 accaaataca cccgttctaa ccagaacacc tgtatcaacc agatgccgtg tgtgtctctg
     6661 ggtgaaccgg ttgaacgtgg cgacgtgctg gcagacggtc cgtccaccga cctcggtgaa
     6721 ctggcgcttg gtcagaacat gcgcgtagcg ttcatgccgt ggaatggtta caacttcgaa
     6781 gactccatcc tcgtatccga gcgtgttgtt caggaagacc gtttcaccac catccacatt
     6841 caggaactgg cgtgtgtgtc ccgtgacacc aagctgggtc cggaagagat caccgctgac
     6901 atcccgaacg tgggtgaagc tgcgctctcc aaactggatg aatccggtat cgtttacatt
     6961 ggtgcggaag tgaccggtgg cgacattctg gttggtaagg taacgccgaa aggtgaaact
     7021 cagctgaccc cagaagaaaa actgctgcgt gcgatcttcg gtgagaaagc ctctgacgtt
     7081 aaagactctt ctctgcgcgt accaaacggt gtatccggta cggttatcga cgttcaggtc
     7141 tttactcgcg atggcgtaga aaaagacaaa cgtgcgctgg aaatcgaaga aatgcagctc
     7201 aaacaggcga agaaagacct gtctgaagaa ctgcagatcc tcgaagcggg tctgttcagc
     7261 cgtatccgtg ctgtgctggt agccggtggc gttgaagctg agaagctcga caaactgccg
     7321 cgcgatcgct ggctggagct gggcctgaca gacgaagaga aacaaaatca gctggaacag
     7381 ctggctgagc agtatgacga actgaaacac gagttcgaga agaaactcga agcgaaacgc
     7441 cgcaaaatca cccagggcga cgatctggca ccgggcgtgc tgaagattgt taaggtatat
     7501 ctggcggtta aacgccgtat ccagcctggt gacaagatgg caggtcgtca cggtaacaag
     7561 ggtgtaattt ctaagatcaa cccgatcgaa gatatgcctt acgatgaaaa cggtacgccg
     7621 gtagacatcg tactgaaccc gctgggcgta ccgtctcgta tgaacatcgg tcagatcctc
     7681 gaaacccacc tgggtatggc tgcgaaaggt atcggcgaca agatcaacgc catgctgaaa
     7741 cagcagcaag aagtcgcgaa actgcgcgaa ttcatccagc gtgcgtacga tctgggcgct
     7801 gacgttcgtc agaaagttga cctgagtacc ttcagcgatg aagaagttat gcgtctggct
     7861 gaaaacctgc gcaaaggtat gccaatcgca acgccggtgt tcgacggtgc gaaagaagca
     7921 gaaattaaag agctgctgaa acttggcgac ctgccgactt ccggtcagat ccgcctgtac
     7981 gatggtcgca ctggtgaaca gttcgagcgt ccggtaaccg ttggttacat gtacatgctg
     8041 aaactgaacc acctggtcga cgacaagatg cacgcgcgtt ccaccggttc ttacagcctg
     8101 gttactcagc agccgctggg tggtaaggca cagttcggtg gtcagcgttt cggggagatg
     8161 gaagtgtggg cgctggaagc atacggcgca gcatacaccc tgcaggaaat gctcactgtt
     8221 aagtctgatg acgtgaacgg tcgtaccaag atgtataaaa acattgtgga cggcaaccat
     8281 cagatggagc cgggcatgcc agaatccttc aacgtattgt tgaaagagat tcgttcgctg
     8341 ggtatcaaca tcgaactgga agacgagtaa ttctcgctca aacaggtcac tgctgtcggg
     8401 gtaaaacccg gcagcggatt gtgctaactc cgacgggagc aaatccgtga aagatttatt
     8461 aaagtttctg aaagcgcaga ctaaaaccga agagtttgat gcgatcaaaa ttgctctggc
     8521 ttcgccagac atgatccgtt catggtcttt cggtgaagtt aaaaagccgg aaaccatcaa
     8581 ctaccgtacg ttcaaaccag aacgtgacgg ccttttctgc gcccgtatct ttgggccggt
     8641 aaaagattac gagtgcctgt gcggtaagta caagcgcctg aaacaccgtg gcgtcatctg
     8701 tgagaagtgc ggcgttgaag tgacccagac taaagtacgc cgtgagcgta tgggccacat
     8761 cgaactggct tccccgactg cgcacatctg gttcctgaaa tcgctgccgt cccgtatcgg
     8821 tctgctgctc gatatgccgc tgcgcgatat cgaacgcgta ctgtactttg aatcctatgt
     8881 ggttatcgaa ggcggtatga ccaacctgga acgtcagcag atcctgactg aagagcagta
     8941 tctggacgcg ctggaagagt tcggtgacga attcgacgcg aagatgggtg cggaagcaat
     9001 ccaggctctg ctgaagagca tggatctgga gcaagagtgc gaacagctgc gtgaagagct
     9061 gaacgaaacc aactccgaaa ccaagcgtaa aaagctgacc aagcgtatca aactgctgga
     9121 agcgttcgtt cagtctggta acaaaccaga gtggatgatc ctgaccgttc tgccggtact
     9181 gccgccagat ctgcgtccgc tggttccgct ggatggtggt cgtttcgcga cttctgacct
     9241 gaacgatctg tatcgtcgcg tcattaaccg taacaaccgt ctgaaacgtc tgctggatct
     9301 ggctgcgccg gacatcatcg tacgtaacga aaaacgtatg ctgcaggaag cggtagacgc
     9361 cctgctggat aacggtcgtc gcggtcgtgc gatcaccggt tctaacaagc gtcctctgaa
     9421 atctttggcc gacatgatca aaggtaaaca gggtcgtttc cgtcagaacc tgctcggtaa
     9481 gcgtgttgac tactccggtc gttctgtaat caccgtaggt ccatacctgc gtctgcatca
     9541 gtgcggtctg ccgaagaaaa tggcgctgga gctgttcaaa ccgttcatct acggcaagct
     9601 ggaactgcgt ggtcttgcta ccaccattaa agctgcgaag aaaatggttg agcgcgaaga
     9661 agctgtcgtt tgggatatcc tggacgaagt tatccgcgaa cacccggtac tgctgaaccg
     9721 tgcaccgact ctgcaccgtc tgggtatcca ggcatttgaa ccggtactga tcgaaggtaa
     9781 agctatccag ctgcacccgc tggtttgtgc ggcatataac gccgacttcg atggtgacca
     9841 gatggctgtt cacgtaccgc tgacgctgga agcccagctg gaagcgcgtg cgctgatgat
     9901 gtctaccaac aacatcctgt ccccggcgaa cggcgaacca atcatcgttc cgtctcagga
     9961 cgttgtactg ggtctgtact acatgacccg tgactgtgtt aacgccaaag gcgaaggcat
    10021 ggtgctgact ggcccgaaag aagcagaacg tctgtatcgc tctggtctgg cttctctgca
    10081 tgcgcgcgtt aaagtgcgta tcaccgagta tgaaaaagat gctaacggtg aattagtagc
    10141 gaaaaccagc ctgaaagaca cgactgttgg ccgtgccatt ctgtggatga ttgtaccgaa
    10201 aggtctgcct tactccatcg tcaaccaggc gctgggtaaa aaagcaatct ccaaaatgct
    10261 gaacacctgc taccgcattc tcggtctgaa accgaccgtt atttttgcgg accagatcat
    10321 gtacaccggc ttcgcctatg cagcgcgttc tggtgcatct gttggcatcg atgacatggt
    10381 catcccggag aagaaacacg aaatcatctc cgaggcagaa gcagaagttg ctgaaattca
    10441 ggagcagttc cagtctggtc tggtaactgc gggcgaacgc tacaacaaag ttatcgatat
    10501 ctgggctgcg gcgaacgatc gtgtatccaa agcgatgatg gataacctgc aaactgaaac
    10561 cgttattaac cgtgacggtc aggaagagaa gcaggtttcc ttcaacagca tctacatgat
    10621 ggccgactcc ggtgcgcgtg gttctgcggc acagattcgt cagcttgctg gtatgcgtgg
    10681 tctgatggca aagccggatg gctccatcat cgaaacgcca atcaccgcga acttccgtga
    10741 aggtctgaac gtactccagt acttcatctc cacccacggt gctcgtaaag gtctggcgga
    10801 taccgcactg aaaactgcga actccggtta cctgactcgt cgtctggttg acgtggcgca
    10861 ggacctggtg gttaccgaag acgattgtgg tacccatgaa ggtatcatga tgactccggt
    10921 tatcgagggg ggtgacgtta aagagccgct gcgcgatcgc gtactgggtc gtgtaactgc
    10981 tgaagacgtt ctgaagccgg gtactgctga tattctcgtt ccgcgcaaca cgctgctgca
    11041 cgaacagtgg tgtgacctgc tggaagagaa ctctgtcgac gcggtaaaag tacgttctgt
    11101 tgtatcttgt gacaccgact ttggtgtatg tgcgcactgc tacggtcgtg acctggcgcg
    11161 tggccacatc atcaacaaag gtgaagcaat cggtgttatc gcggcacagt ccatcggtga
    11221 accgggtaca cagctgacca tgcgtacgtt ccacatcggt ggtgcggcat ctcgtgcggc
    11281 tgctgaatcc agcatccagg tgaaaaacaa aggtagcatc aagctcagca acgtgaagtc
    11341 ggttgtgaac tccagcggta aactggttat cacttcccgt aacaccgaac tgaaactgat
    11401 cgacgaattc ggtcgtacca aagaaagcta caaagtgcct tacggtgcgg tactggcgaa
    11461 aggcgatggc gaacaggttg ctggcggcga aaccgttgca aactgggacc cgcacaccat
    11521 gccggttatc accgaagtaa gcggttttgt acgctttact gacatgatcg acggccagac
    11581 cattactcgt cagaccgacg aactgaccgg tctgtcttcg ctggtggttc tggattccgc
    11641 agaacgtacc gcaggtggta aagatctgcg tccggcactg aaaatcgttg atgctcaggg
    11701 taacgacgtt ctgatcccag gtaccgatat gcctgcgcag tacttcctgc cgggtaaagc
    11761 gattgttcag ctggaagatg gcgtacagat cagctctggt gacaccttgg cgcgtattcc
    11821 gcaggaatcc ggcggtacca aggacatcac cggtggtctg ccgcgcgttg cggacctgtt
    11881 cgaagctcgt cgtccgaaag agccggcaat cctggctgaa atcagcggta tcgtttcctt
    11941 cggtaaagaa accaaaggta aacgtcgtct ggttatcacc ccggtagacg gtagcgatcc
    12001 gtacgaagag atgattccga aatggcgtca gctcaacgtg ttcgaaggtg aacgtgtaga
    12061 acgtggtgac gtaatttccg acggtccgga agcgccgcac gacattctgc gtctgcgtgg
    12121 tgttcatgct gtgactcgtt acatcgttaa cgaagtacag gacgtatacc gtctgcaggg
    12181 cgtaaagatt aacgataaac acatcgaagt tatcgttcgt cagatgctgc gtaaagctac
    12241 catcgttaac gcgggcagct ccgacttcct ggaaggcgaa caggttgaat actctcgcgt
    12301 caagatcgca aaccgcgaac tggaagcgaa cggcaaagtg ggtgcaactt actcccgcga
    12361 tctgctgggt atcaccaaag cgtctctggc aaccgagtcc ttcatctccg cggcatcgtt
    12421 ccaggagacc actcgcgtgc tgaccgaagc agccgttgcg ggcaaacgcg acgaactgcg
    12481 cggcctgaaa gagaacgtta tcgtgggtcg tctgatcccg gcaggtaccg gttacgcgta
    12541 ccaccaggat cgtatgcgtc gccgtgctgc gggtgaagct ccggctgcac cgcaggtgac
    12601 tgcagaagac gcatctgcca gcctggcaga actgctgaac gcaggtctgg gcggttctga
    12661 taacgagtaa tcgttaatcc gcaaataacg taaaaacccg cttcggcggg tttttttatg
    12721 ggtgttattc agagggcaaa ttcttcacgg aaaatattaa gaaaggcagg gcgaaacgag
    12781 gaagaagctt ttcgccctgg acaacattcc tgctgacggc actaccataa aaaaacacaa
    12841 cgctttcaac aaccttgcgt ttttcatctt ccgtagcggc tcggaataaa tttacgttct
    12901 tacattccgt ctcatagtct ttgcgaggcg cgtcccagat agctgtccca gtctttccat
    12961 accggctgca aaccctgagc ggttaacacg gcagcaaccg cttccggtct gcgatcgtcg
    13021 tgcggtgaga actgttccaa ctcggggtga ttatcggcat agccacctgg ctgcgttttc
    13081 gagaaggcgc tgacgttatt gatcgccagc ggaataacgc gatcgcgaaa ccacggtgat
    13141 tcccgcgtgg agagtgacag ttcaatctcc ggtgcaagca gtcggaaggc gcagatggtt
    13201 tgcactaact ggcgttcatc cataatcgac gcaggctcaa tgccgccagt acacgggcgc
    13261 aggcgcggaa aggagacaga gtaacggctt tgccagtaat gctgttgcag ccatagcaaa
    13321 tgttcggcaa ccatatagca gtcaacgcgc cagttgtcgg aaaggccaat tagcgcgccg
    13381 aggcctatct tatcaatccc cgcacgcccc agccgatccg gcgtttccag ccgccagaag
    13441 aagtcctgtt ttttgccttt caaatgatgg cgggtataag tcgcctcgtg atatgtctcc
    13501 tgataaacca tcaccccatc cagaccaagt tgctttaact cggcgtattc cgtctccgcc
    13561 agcggttgca cttccatttg tagtgaagag aactgttcac gcagggcggg gagatgacga
    13621 cgaaagtaat ccatccccac tttcgcctga tgttcaccag tgactaatag cagatgttca
    13681 aagcccatct cccgtatagc ggcgctttcc ctggcaatat ccgcttcatc cagcgttttg
    13741 cgcttgatgc gattactcat ggaaaatccg cagtacgtgc agtcgttagc gcaaagattg
    13801 gaaagataaa gcgggacgta gaaactaact gtgttgccaa atcgctgacg ggtcagacgc
    13861 tgcgcctgtt gggccagttg ttccagatag ccactggcgg caggcgataa cagcgccatc
    13921 atgtcgtcgc gggtgagttg cgaggcattt agcgcccgct ctacgtcagc agccgttttg
    13981 ccgttgatac gcaggcggat gtcgtcccag tccagttgtc gccagcgatt gctgaaggtt
    14041 ttcatgccga tgcctccaga aatccggtca gcgggctggt ggcatgagca aaataactgc
    14101 ggctgcccgg tccggactga cgtgccagca ggcctacttc taccgccaga cgaaatgcct
    14161 tcgccatgtt gacgggatcg tccgcgacgg caatcgccgt attcactaac accgcgtcgg
    14221 cccccatttc cagcgcctgc gtggcatggc tgggaacgcc gatgccagca tcgacaacca
    14281 ccggaactgt ggcctgctgg ataataatct ccagcatggc acgggtttcc agtccctgat
    14341 tcgagccaat cggcgcgccg agcggcatca ccgctgcaca gccgacttct tccagacgtt
    14401 tacacaatac cggatcggcc ccgcagtaag gcagcacgac aaatccctgt tgtaccagcg
    14461 tttcggcggc tttcagggtt tcgatgggat cgggcaacag ccagcgggcg tcagggtgaa
    14521 tctccaattt tagccagttt gtgcctaacg cttcacgtgc cagatgggcg gcgaaaatgg
    14581 cttcttccgc tgttttcgcc ccggaagtat tcggcagcag ggccacaccc gccgcgataa
    14641 gcggttcgag gatagcgtcg ttgtgctggc gcaagtcgac acgtttcatc gccagtgtca
    14701 ccagctggct gccggaagcg cggatcgcct ccaccatcag ttgtgaagaa gcgaatttcc
    14761 ctgtgccggt aaacagatgt gaatcaaacg ttttgtccgc aatacgtaac atctcaaccc
    14821 cctgcaataa cctgaaaaag caggatctgg tcgccatcct gcacgatatg ttgcgtccac
    14881 tgctcacgcg ggacgatttg ctgattaatc gccagagccg cgcccgcttg tcgttggtcg
    14941 agttgctcca gtagttcgtg aacagtttgc ccggcggcgc actgcatcgg ttgatcgtta
    15001 aacaggatct gcattgcttc ctccgcatac cgggcaacca ctggcgcggc gcaacgccag
    15061 gctgcgccac tggctcgact taccgtcgaa cagtcggagt tctcccgcag gcgtctctat
    15121 accgcttaat aacttaatgg cttccagtgc ctgcaaagtg cccatcaccc cgaccaccgg
    15181 gccaaccacg cccgccgtgc ggcagttgcg ttctggctgc tggttatctg gccacaggca
    15241 gcggtaacac ccctgctccc agggcggtgt cagtaccatc aactgaccgc caaatccgac
    15301 cgcgctggcg gtgataagcg gcgtgttgag tgccacgcag gcggcattaa tctcctggcg
    15361 agtcgccata ttgtcggtac agtcgagcac cacatcggct tgtgcaaccg catctttcaa
    15421 cgcctcaccc gttaaccgtt gttgtaatgc tgtcagttga atgtcgggat tcaactgtgt
    15481 cagtcgctgt tggctgacct gcgatttcgg gctgttgata tcttcagtgg taaagaggat
    15541 ttgtcgttgc agattgctta aatgcacatc gtcgtcatct gccagtacca gcgtcccgac
    15601 gccagcccct gccaggtata gcgcagcagg tgtacccagc ccgccaagac caataatcag
    15661 cacctggctg tcgagcagtt tttgctgccc gtcaagagcg atatcgtcga gcagaatttg
    15721 gcggctataa cgcataaagt cacggtcatt catcgccaac tcctgcaatt tccagcaact
    15781 gtgccgttgc caaacgccag tctgcggctt gagtaatggc gctgacgacg gcgatactgc
    15841 cgacacccgt tgctatcacc gcaggcgcgc gtgccagact gataccgcca atcgccacgg
    15901 tgggataatc cgccaggcgc tcaacatgcc gtgccaactg ttccagcccc tgcggtgcag
    15961 aaggcatctg tttggtttgc gtcgggaaca catgtcccag cgcgatataa gaggggcgtg
    16021 ctgccagcgc gacgtcgatt tccatatcgt catgtgtcga aacgcccagc cgcaggcctg
    16081 ccgcgcggat ggcattgaga tcggtggctt gcaaatcttc ctgccccaaa tggacgccat
    16141 acgcctgatg cttgatcgcg agccgccagt aatcgttgat aaacaatcgc gcgttatagc
    16201 ggcggcccag cgcaattgcc gccacgacat cggcttccac ctcttcatcg cgccgatctt
    16261 tgatgcgtag ctggagagta cgtacgcctg catccaacag acgttcgatc cactgtacgc
    16321 tgtccaccac cgggtacagt cctaaacgaa aaggtacagg aggaaaatca ggctgataca
    16381 tcacgcttcc tccttatgca ggtagatttc tccgcctctg gcacggaagt tctccgacat
    16441 atccgccatt cccacttcaa tggtttgcgc ggcggcgtaa tcgcgtactt cctggctgat
    16501 tttcatcgag cagaattttg gcccacacat ggagcaaaaa tgggcgactt tgccggactc
    16561 ttggggcagg gtttcatcgt gataagcgcg ggcggtaaac gggtcgaggg ccagattaaa
    16621 ctggtcttcc cagcgaaatt cgaagcgggc tttcgacatg gcgttatcgc gaatttgcgc
    16681 gcccggatgc ccttttgcca ggtcagcagc gtgggcggca atcttataag tgataagccc
    16741 ctgcttaaca tcttctttat tgggcagacc cagatgctct tttggcgtta cgtaacagag
    16801 catcgcgcag ccaaaccagc caatcatcgc cgcaccaatc cccgacgtga agtggtcata
    16861 gcccggtgca atatcggtag ttagcggccc cagagtgtaa aacggcgctt cgtggcagtg
    16921 ctctaactcc tcggtcatat tgcggcggat catctgcatc ggcacatggc ctgggccttc
    16981 aatcatcacc tgtacgtcat attcccaggc gattttggtc agttcgccca gcgtatgcaa
    17041 ttcggcaaac tgcgcttcat cgttggcgtc ctgaatagaa ccggggcgca gaccgtcgcc
    17101 cagcgacagc gaaacgtcat aagcggcaca gatttcacaa atttcgcgga agtgttgata
    17161 gaggaaattt tcctgatgat gggagaggca ccatttcgcc ataatcgaac cgccgcgaga
    17221 gacgataccg gtcaggcgtt tcgcggtcat cggcacatag cgcagcagta cgcccgcatg
    17281 gatagtgaag taatccacac cttgctcggc ctgttccagc agcgtgtcgc ggaacgcttc
    17341 ccaggtaaga tcttcggcga tcccgttaac cttctccagc gcctggtaga tcggcactgt
    17401 accgatcggc accggactgt tacgcaaaat ccactcgcgg gtttcgtgaa tatagcgacc
    17461 ggtggagaga tccatcaccg tatccgctcc ccagcgcgtg gaccatacca gcttttccac
    17521 ttcttcttcg atggaagagg tgaccgccga gttgccgata ttggcgttaa cttttaccag
    17581 gaagttacgg ccaataatca tcggctccga ttccggatga ttaatgttgg ccgggataat
    17641 cgcacgtccg gcagcaactt catcacggac aaattccgca gtgatatttt ccggcagatg
    17701 tgcgccaaag ctcattcccg gatgctggtg gcgtaaaacc tcgctacgga tgcgctcgcg
    17761 gcccatattc tcgcggatgg cgatgaattc catttccggc gtgatgatgc cctggcgggc
    17821 gtagtgcagt tgggtgacac ggcgtcctgt tttggcgcgt tttggcgtta ataagccgct
    17881 aaaacgtagc tcgtcgagac catcatctgc cagccgcgtt ctggtgtaat cggaactgcg
    17941 cacggtaagt tcttcggtat cggcgcgcgc atcgatccac ggctggcgta gttttgccag
    18001 cccttgctgc acgttaatgg cgatctgcgg atcaccatac gggccggagg tgtcgtagac
    18061 cggaatcgct tcgttttctt cgtactgcgg ctgttctttg ctaccgccaa ttagcgtcgg
    18121 gctaagctgg atctcacgca tcggcacgcg cacgccgggg tgtgtgccag tgatataaat
    18181 gcgttttgag ttgggaaagg cggtgccttc cagggtgtcg ataaaatgtt gggcccgggc
    18241 gcgttgttcg cggcgggtca gttttgttgc agacatagct cattccaaaa agttaaggac
    18301 gtggcttgtc agacgacgga tgaagcaaga gacgatcgcc gcaaaggcga tgcgatagca
    18361 gattaactct tgttcccttc gcaggtatta gcctgatcag gttccgcgga tcccgaataa
    18421 acggtctcag ccagttaagg cactccgaca agaaattagc ccccacaaac ggggcgttga
    18481 atgtaaatta cgcgttaaca gcgcagaact caagcaggat gtttgacgcg ggcggcgtca
    18541 agcaccagca gaatcagctt atcttccagc acaaagcgcg cttccagcgc ctcgccgatg
    18601 tcagataaaa cctgttgaaa ctcaaggtaa ttatcatgat cgatggcggt ttccaggctg
    18661 gaatcgtagt aatccataat ctgttgggta ttggcttcga gttgcggcca aatcttcgcg
    18721 gcgcgcgcga gttgcccgtt gccttccagc ttatgaagaa tgcgctcata aatactgaaa
    18781 tgtccggcag acaagtaatc gaccaggctc tgacaaaaat catcaagggc tttttcgttt
    18841 agcctcatgt acgattcttt gccaggctta atgccaacca gattgtagta agccacgagc
    18901 agatgcttac gtacatgtag ccagcgatca accagtttgt tacttcctct gacgcgctcc
    18961 gtcaggttat cgagctggtt aagcatgatt gactccgcaa gtttgtattc aaaaactgct
    19021 cagtgagaaa tgtaaaaacc atgttaaaca tgccagtgat gcaaaggtag tgcaagagct
    19081 atggatcgta taattgaaaa attagatcac ggctggtggg tcgtcagcca tgaacaaaaa
    19141 ttatggttgc cgaagggaga attgccatat ggcgaagcgg caaatttcga tcttgtgggt
    19201 cagcgcgcac tacaaatcgg cgaatggcag ggggaacctg tttggttaat acaacagcag
    19261 cggcgtcacg atatggggtc ggtacgtcag gtcattgatc tcgatgttgg gctgtttcaa
    19321 ctggccggac gtggcgtgca actggcggag ttttaccgat cgcataaata ctgtggttac
    19381 tgcgggcatg aaatgtatcc gagcaaaacc gaatgggcga tgctgtgtag ccattgccgt
    19441 gagcgttact acccgcaaat cgccccctgc attattgttg ccatccgtcg cgatgattcg
    19501 ctcctcctcg cccagcatac ccgccatcgt aacggtgtcc atacagtact tgccggattc
    19561 gtcgaagtgg gcgaaaccct cgagcaggca gtcgcgcggg aagtgatgga agagagcgga
    19621 attaaagtta aaaacttgcg ttacgtgact tctcagccgt ggccgtttcc tcagtcttta
    19681 atgaccgcgt ttatggcgga atatgacagc ggcgacatcg tgatcgaccc gaaagaattg
    19741 ctcgaggcga actggtatcg ctatgacgat ttgccgttac tcccgccgcc cggcaccgta
    19801 gcgcgccgtc tgatagaaga tactgtggcg atgtgtcggg cagagtatga gtgatgatac
    19861 actgaccgcc tgacgcacta aggaacagcc aaaatgaccg aacttaaaaa cgatcgttat
    19921 ctgcgggcgc tgctgcgcca gcccgttgat gtcactccag tatggatgat gcgccaggcg
    19981 ggtcgctatc taccggaata taaagccacg cgcgcccagg cgggcgattt tatgtcgctg
    20041 tgcaaaaacg ccgagctggc gtgcgaagtg actttgcaac cgctgcgtcg ctacccgctg
    20101 gatgcggcga tcctcttttc cgatatcctc accgtgccgg acgcgatggg gttagggctc
    20161 tattttgaag ccggagaagg tccgcgtttt acctcgccag tcacctgcaa agccgacgtc
    20221 gataaactgc caattccgga cccggaagat gagctgggtt acgtgatgaa cgcggtgcgt
    20281 accattcgtc acgaactgaa aggcgaagtg ccgctgattg gtttttccgg cagcccgtgg
    20341 acgctggcaa cctacatggt ggaaggcggc agcagcaaag ccttcaccgt gatcaaaaaa
    20401 atgatgtatg ccgatccgca ggcgctgcac gctctgctcg ataaactggc gaaaagcgtc
    20461 actttgtatc tgaatgcgca gattaaagcc ggtgctcagg cagtgatgat tttcgacacc
    20521 tggggcggcg tgcttaccgg gcgcgattat caacagttct cgctctatta catgcataaa
    20581 attgttgatg gtttactgcg tgaaaacgac ggtcgccgcg tgccagtcac gctgtttacc
    20641 aaaggcggcg gacagtggct ggaagccatg gctgaaaccg gttgcgatgc gctgggcctc
    20701 gactggacaa cggacatcgc cgatgcgcgc cgccgtgtgg gcaataaagt cgcgttgcag
    20761 ggtaatatgg atccgtcgat gctgtacgcg ccgcctgccc gcattgaaga agaagtagcg
    20821 actatacttg caggtttcgg tcacggcgaa ggtcatgtct ttaaccttgg tcacggcatt
    20881 catcaggatg tgccgccaga acatgctggc gtgttcgtgg aggcagtgca tcgactgtct
    20941 gaacagtatc accgctaaga gcctgatcca tcaggctatt ttatttgcca ttttgaggcc
    21001 gggcagtgct caaaatcctc acgtactacg tgtacgctcc ggtttttccg cgctgtccgg
    21061 cctcaaactg ccttcaacaa ttacgcctgt tgaaccaggc tctaaggagc gattatggat
    21121 ctcgcgtcat tacgcgctca acaaatcgaa ctggcttctt ctgtgatctg cgaggatcga
    21181 ctcgataaag atccaccgga tctgatcgcc ggagccgatg tcgggtttga gcagggcgga
    21241 gaagtgacgc gagcggcgat ggtgctgctg aaatatccct cgcttgagct ggtggagtat
    21301 aaagttgccc gcatcgccac caccatgcct tacattccag gttttctttc cttccgcgaa
    21361 tatcctgcgc tgctggcagc gtgggagatg ctgtcgcaaa agccggattt agtgtttgtc
    21421 gatggtcatg ggatctcgca tcctcgccgt cttggcgtcg ccagccattt tggcttattg
    21481 gtggatgtgc cgaccattgg cgtggcgaaa aaacggctct gcggtaaatt cgaaccgctc
    21541 tccagcaaac cgggcgcgct ggccccactg atggataaag gcgagcagct ggcctgggtc
    21601 tggcgcagca aagcgcgctg taacccgttg tttatcgcta ccggccatcg ggtcagcgtg
    21661 gacagcgcgc tggcgtgggt acaacgctgc atgaaaggct atcgtctgcc ggagccaacg
    21721 cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac agcaaatcag
    21781 ccctaattca ggtaaactgc ggataatttc cgtatttgag aactcaacat gttacaaaac
    21841 ccaattcatc tgcgtctgga gcgcctggaa agctggcagc atgtcacttt catggcttgc
    21901 ttatgcgaac gcatgtaccc caattacgcc atgttctgcc agcaaaccgg ttttggtgat
    21961 gggcaaattt accgtcgtat tctcgatctc atctgggaaa cgctgaccgt taaagacgca
    22021 aaagtaaatt tcgacagcca actggagaaa tttgaagaag cgattccttc agccgacgat
    22081 ttcgatctgt acggcgttta tccggcaatc gatgcctgcg tggcgttaag tgaactggtc
    22141 cattcgcgtt tgagtggtga aacgctcgaa cacgcggtgg aagtgagtaa gacctccatc
    22201 acgaccgttg cgatgctgga aatgactcag gctggtcgcg aaatgggcga tgaagagctt
    22261 aaagaaaacc cagctgtaga gcaagaatgg gacattcagt gggaaatatt ccgactttta
    22321 gctgagtgcg aagaacgcga tatcgagctg ataaaaggcc ttagggcaga cctgcgtgag
    22381 tcgggtgaga gcaatattgg tataattttt cagcaataag accagaaaac gtgatttaac
    22441 gcctgatttg tcgtacctgg agtcttccct ttcgcccccc gtctggtcta catttggggg
    22501 gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt gctattctgg catttccgtc
    22561 gcactcgatg cttagcaagc gataaacaca ttgtaaggat aacttatgaa caagactcaa
    22621 ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa agctgctctg
    22681 gagtccactc tggctgcaat tactgagtct ctgaaagaag gcgatgctgt acaactggtt
    22741 ggtttcggta ccttcaaagt gaaccatcgc gctgagcgta ctggccgcaa cccgcagacc
    22801 ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg caaggcactg
    22861 aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg ggtggtttca
    22921 ccccttttgt ctttctggcg tcgatcattg atgctggcag gtgcgctgtt tctcactgcc
    22981 tgtagccaca actcttcact tcctcccttt accgccagtg gatttgctga agaccagggc
    23041 gcggtacgca tctggcgaaa agacagcggc gataatgtgc atctgcttgc cgtgtttagc
    23101 ccgtggcgca gtggcgatac cacgacgcga gagtatcgct ggcagggcga taacctcacg
    23161 ctcatcaata tcaatgttta cagcaaaccg ccggtgaaca ttcgcgcgcg ttttgacgat
    23221 cgcggcgatc tgagctttat gcaacgtgaa tccgatgggg aaaagcagca gctttctaac
    23281 gaccaaatcg atttataccg ttatcgtgct gatcagatcc gccagattag cgatgcctta
    23341 cgtcagggga gagtcgtgct gcgccagggg cgctggcatg cgatggaaca gaccgtgacc
    23401 acctgcgaag ggcaaaccat taaacctgat ttagattcgc aggcgatagc gcatatcgag
    23461 cgccgccaga gccgctcttc tgttgatgtc agcgtggcat ggctggaagc gcccgaaggt
    23521 tcgcaattac tgttagtggc aaactctgat ttctgtcgct ggcaacccaa cgagaaaacg
    23581 ttctgattta ccagtggccc atacccatat gaccgccacc accgcagccg ccgtagccca
    23641 tgcccattcc ggctccgcgc ggaatacccg cttcagccat cgcgatatct cgtttcaccc
    23701 gtaactcatc taacgactga cgcaaattct ccatctcttt ggcgaccgcg ttaattttgc
    23761 tgctatccgg tgggttcgcg gctaacaggg cattgtattc ataacgcttc gtcaccagtt
    23821 gctgttgcag tgcgctgctt tgagcgtaaa agtcattatg gattttctgc cacgctgtct
    23881 gttgttcgct ggtcaaaggc gcggcatttt gctgccacat accgtgtccg ccgtgagcaa
    23941 atgcagatgt cgatcccatc gccattgctg aaagcgccat cattaccagg gcaattttcg
    24001 tgttccgttt catggttaat cctccagtgg ttgtcttact tcgggtattg catcttccgt
    24061 gccaacgatg aaacgctgat atgacgggta atctggcatg ataaacgagt aaaaatgact
    24121 cgcctgctgc gggtagcgag tcatttttac tcattgaaac ttaagccttt gtgttacagc
    24181 gcagggtaag cgctgataaa agatggcatg atttctgctg tcagaaaggg atgagcaggc
    24241 aaagaagaag atgcgtttta tgcaacgttc taaagactcc ttagctaaat ggttaagcgc
    24301 gatcctcccc gtggtcattg ttgggctggt agggttgttt gcggtgacgg tgattcgcga
    24361 ttatgggcgc gagactgccg ccgccagaca aacgctgctg gaaaaaggca gtgtacttat
    24421 tcgcgctctt gaatccggct cgcgcgtcgg catggggatg cgcatgcatc atgcgcagca
    24481 gcaggcctta ctggaagaaa tggccgggca gcctggagta cgttggtttg cggtcacgga
    24541 tgaacaagga acaatcgtga tgcatagcaa ctccggcatg gtgggaaaac agctttattc
    24601 ccctcaggaa atgcagcagt tacatccggg agatgaagaa gcgtggcggc ggatcgatag
    24661 cgcagacggc gagcctgttc tggaaattta tcgccagttt caaccgatgt ttgctgctgg
    24721 aatgtaccgg atgcgccata tgcagcagta tgccgcgaca ccacaagcaa ttttcatcgc
    24781 tttcgacgcc agtaatattg tgagtgccga agatcgtgag cagagaaaca ccctgattat
    24841 tctcttcgcc ctggcgacgg tcttgctggc aagcgtattg tcattcttct ggtatcgccg
    24901 ctatctgcgc tcgcgccagc ttctacaaga tgaaatgaag cgcaaagaga agctggtggc
    24961 gctggggcat cttgcggcag gcgttgccca cgaaatccgt aacccacttt cctcgattaa
    25021 aggactggcg aaatactttg ccgagcgcgc gcctgcaggg ggagaagcgc atcaactggc
    25081 gcaggtgatg gcgaaagagg ccgaccgttt aaaccgcgtg gtaagcgagt tgctggaact
    25141 ggttaagcca acgcatctgg ctttgcaggc ggtggatctc aacacgctga ttaaccactc
    25201 attacagctg gtaagccagg atgcaaacag ccgggagatc cagttacgct ttaccgccaa
    25261 cgacacatta ccggaaattc aggccgatcc ggacaggctg actcaggtcc tgttgaatct
    25321 ctatctcaat gctattcagg cgattggtca gcatggcgtg attagcgtga cggccagcga
    25381 aagcggcgcg ggcgtgaaaa tcagcgttac cgacagcggt aagggaattg cggcagatca
    25441 gcttgaagcc atcttcactc cgtacttcac caccaaagcc gaaggcaccg gattggggct
    25501 ggcggtcgtg cataatattg ttgaacaaca cggtggtaca attcaggtcg caagccagga
    25561 gggaaaaggc tcaacgttca ccctctggct tccggtcaat attacgcgta aggacccaca
    25621 aggatgacgc acgataatat cgatattctg gtggtggatg atgacattag ccactgcact
    25681 attttgcagg ctttactgcg cggctggggc tataacgtcg cgctggcgaa cagcgggcga
    25741 caggcgctgg agcaggtgcg ggaacaggtt tttgatcttg tgctttgcga tgtgcgaatg
    25801 gcggagatgg acggcatcgc cacgctgaaa gagatcaaag cgttaaaccc ggcaattccg
    25861 gtgctgatta tgactgcgta ctccagcgtc gagacggcgg tagaggcact gaaaactggg
    25921 gcgcaggatt atctcatcaa gccgctggat ttcgataacc tacaggcgac gctggaaaag
    25981 gcgctcgcgc atacgcacag tattgatgct gaaacacctg cggtgactgc cagccagttc
    26041 ggtatggtcg gtaaaagccc ggcgatgcaa cacctgctca gtgaaatcgc cctcgtcgcg
    26101 ccatcggaag ccacggtgct gatccacggc gattccggca ccggtaaaga gctggtcgcc
    26161 agggcgattc acgccagtag cgcacgtagc gaaaaaccgc tggtaacgct caactgtgcg
    26221 gcactcaacg aatccttgct ggaatctgaa ttgttcggtc acgaaaaagg ggcgtttact
    26281 ggagccgata aacgccggga agggcgcttt gttgaggcgg acggcggcac gctgtttctc
    26341 gatgaaattg gcgatatctc gccgatgatg caggtgcgtc tgctgcgtgc gattcaggag
    26401 cgcgaagttc agcgtgtcgg tagcaaccag actatctcgg ttgatgtccg gctgattgcg
    26461 gcgacccatc gcgatcttgc cgcagaggtg aatgccgggc gttttcgcca ggatctctac
    26521 tatcgcctga atgtggtggc gattgaagta ccatcgctgc ggcaacggcg ggaagatatc
    26581 cctctgctgg ctggccattt tcttcagcgc tttgccgagc gtaatcgcaa ggcggtaaaa
    26641 ggctttacgc cacaggcgat ggatttgctg atccactacg actggccggg aaatattcgt
    26701 gaactggaaa acgcggtgga acgggcggtg gtgctgctga ccggggaata tatttccgaa
    26761 cgcgagctgc cgctggcgat tgccagtacg ccgatcccgc tggggcaaag tcaggatatt
    26821 cagccgctgg tggaagtgga aaaagaggtg attctggcgg cgctggagaa aacgggcggc
    26881 aacaaaaccg aagccgcccg tcagttaggg atcacgcgca aaacgctatt ggcaaaactg
    26941 tcgcgttagt tctgctcgcg ttcgatggcg cgccaaccga tatctttccg gcagaagcaa
    27001 tcatcccagt ggatatcggt cattaaggcg taggcgcgtt tctgcgcttc tgccacggta
    27061 tgacccagcg cggtgacgca cagtacgcgc ccgccgctgg ttaccacctg ctcgtcatcc
    27121 gccagttttg tgcccgcgtg gaacactttg ccgtcagcca cttcttccag cggcaggccg
    27181 tggatcacat cgccggtgcg gtaatcgccc ggatatccac ccgcagccat caccacgctg
    27241 agagaagcac gttcatccca ctctgacgtt ttctcgtcca gtttgccttc acaggccgcc
    27301 aggcagagct caaccagatc ggacttcatg cgcagcataa tcggctgggt ttccggatcg
    27361 ccaaagcggc agttaaattc aataaccttc ggattgccct gtttgtcgat catcaggccc
    27421 gcatagagaa aaccggtgta ggtgttgcct tccgccgcca tgcctttcac ggttggccag
    27481 atgatacgtt ccatggtgcg ctgatgaact tcatcggtta ctaccggcgc gggggagtaa
    27541 gcgcccatcc cgccggtgtt tggtccgtta tctttatcgc ctacgcgttt gtgatcctgg
    27601 ctggtagcca tcggcagcac atgctcgccg tccaccatca cgataaagct cgcttcttcg
    27661 ccatcgagga actcttcgat aacgatgcga tgacccgcgt cgccaaaagc gttgcccgcc
    27721 agcatatcgt gaacagccgc ttccgcttct tccagcgtca tcgccacgat aacgcctttc
    27781 ccggcagcca gaccgtccgc tttaatgacg attggcgcgc ctttctcacg cagatacacc
    27841 agcgcaggtt ctacctcggt gaagttctgg tattccgccg tagggatctt atggcgggcc
    27901 aggaaatctt tggtaaacgc ttttgagcct tccagttggg ccgcacctgc ggttgggccg
    27961 aagattttca gcccggcggc gcggaaggta tcgaccacgc ctttcaccag cggcgcttcc
    28021 gggccgacga tggtcagatc aatcttttcg ttttgtgcga aatccaacag cgccgggata
    28081 tcggtcacgc cgatagcaac gttttgcagc gcgggttcca gcgcagtgcc tgcattaccc
    28141 ggagcagcaa aaacagtctc aaccagcggc gactgggccg ctttccaggc cagcgcgtgc
    28201 tcgcgcccgc cgttaccaat cactaatact ttcatctatt gctccattaa tggcggaagt
    28261 ggcgcatgtc ggtgaagagc atcgcaatac cgtgctcgtc ggcggcggca atcacttcgt
    28321 catcacggat ggaaccgcca ggctggatta cgcaggtcac gcccgcagcg gcggcggcat
    28381 caataccgtc gcggaacggg aagaacgcgt cagaagccat cgaggaacct ttcacttcca
    28441 ggccttcatc ggcagcttta ataccggcga ttttcgcgga gtacacgcgg ctcatctggc
    28501 ccgcgccaat accgatggtc atattgtttt tggcatagac gatagcattg gatttcacga
    28561 atttcgccac tttccagcag aacagcgcat cacgcagttc ctgttcggtc ggctgacgtt
    28621 gggtgacgac gcgcagttct tccgcaccga ccatccccag gtcacgatcc tgaaccagca
    28681 gaccgccgtt cacgcgtttg aaatcaagac ccggaacacg ctcgccccac tgaccgcagg
    28741 tcagaacgcg tacgttctgc ttggcggcgg tgattttcag ggcttcttcg ctggcggaag
    28801 gcgcaataat cacttcgaca aactgacgag aaatgatggc ctgtgcggtt tccgcatcca
    28861 gctcgcggtt aaaggcaatg atgccgccga atgcggaggt tgggtcggtt ttgtacgcgc
    28921 gatcgtaagc atcaagaatg gaattgccga tagctacacc gcaagggttg gcgtgcttca
    28981 caatcacaca tgccggctcg gcgaactctt tcacgcactc cagcgccgca tcggtatcgg
    29041 cgatgttgtt ataagagagg gctttaccct gaacctgggt tgcggtagca acggaggctt
    29101 ctttcacatt ctcttctata tagaaggcag cctgctggtg gctgttctcg ccgtaacgca
    29161 tatcctgctt cttaatgaag ttcaggttca gcgtgcgtgg gaagcgaccg gcggcttctt
    29221 tgctttcacc gtggtacgcc ggaaccatgc tgccgaagta gttggcaatc atgctgtcgt
    29281 aggcggcagt gtgttcgaag gctttgatgg cgaggtcgaa acgggttgca agcgtcagcg
    29341 atccttcgtt gtcatccatc tctttaataa tggcgtcgta gtcgctgctc ttcaccacga
    29401 ttgcgacatc tttatggttc ttagcggcgg agcgcaccat ggtcgggccg ccgatatcga
    29461 tgttctcaac cgcatcttcc agcgagcagc cttcacgagc aacagtctgg gcgaacggat
    29521 acaggttaac aaccaccata tcgataggct ggatctgatg ttcttccata atggcatcgt
    29581 cctggccgcg acggcccaga atgccaccat gtactttcgg atgcagggtc ttcacgcgtc
    29641 catccatcat ctccgggaaa ccggtgtaat cggaaacttc ggttaccggc agaccttttt
    29701 ctgctaacag acgggcagtg ccccctgtag acagcagctc cacaccgcgt gcggaaagtg
    29761 cctgggcgaa ttcgacgata ccggctttgt cagaaacact gagcagagcg cggcggactg
    29821 gacgacgttg ttgcatggta aatcccctgg gtttgactat tacagagagc gttagctgaa
    29881 tttttcgcga aaaactcagc taacgcccct aacggggcat ccttattttt cgcccgcatt
    29941 gtaacgaaaa cgtttgcgca acgctcgcga atttttctct ttcaatggtg atcacaattt
    30001 tgacagtggt taccgtggga aaaatacaga aattacattg atgattgtgg ataactctgt
    30061 gcgtaaaaag gtataaagcg gggttttgct ggggaatgca gcagtcagtc atttttctgc
    30121 aatttttcta ttgcggcctg cggagaactc cctataatgc gccttcatcg acacggcgga
    30181 tgtgaatcac ttcacacaaa cagccggttc ggttgaaga
//
LOCUS       DAZPEU010000028        29855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPEU010000028 DAZPEU010000000
VERSION     DAZPEU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.028"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            15..371
                     /locus_tag="ACHU2L_001191"
     CDS             15..371
                     /locus_tag="ACHU2L_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR1356136.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            375..1502
                     /gene="dapE"
                     /locus_tag="ACHU2L_001192"
     CDS             375..1502
                     /gene="dapE"
                     /locus_tag="ACHU2L_001192"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR1356137.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1530..1730
                     /locus_tag="ACHU2L_001193"
     CDS             1530..1730
                     /locus_tag="ACHU2L_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR1356138.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1840..2538)
                     /gene="ypfH"
                     /locus_tag="ACHU2L_001194"
     CDS             complement(1840..2538)
                     /gene="ypfH"
                     /locus_tag="ACHU2L_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1356139.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2612..4627)
                     /gene="tmcA"
                     /locus_tag="ACHU2L_001195"
     CDS             complement(2612..4627)
                     /gene="tmcA"
                     /locus_tag="ACHU2L_001195"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR1356140.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4642..5505)
                     /locus_tag="ACHU2L_001196"
     CDS             complement(4642..5505)
                     /locus_tag="ACHU2L_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR1356141.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5673..6386)
                     /gene="purC"
                     /locus_tag="ACHU2L_001197"
     CDS             complement(5673..6386)
                     /gene="purC"
                     /locus_tag="ACHU2L_001197"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR1356142.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6599..7633)
                     /gene="bamC"
                     /locus_tag="ACHU2L_001198"
     CDS             complement(6599..7633)
                     /gene="bamC"
                     /locus_tag="ACHU2L_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR1356143.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7650..8528)
                     /gene="dapA"
                     /locus_tag="ACHU2L_001199"
     CDS             complement(7650..8528)
                     /gene="dapA"
                     /locus_tag="ACHU2L_001199"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR1356144.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8674..9246
                     /locus_tag="ACHU2L_001200"
     CDS             8674..9246
                     /locus_tag="ACHU2L_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR1356145.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9246..9716
                     /gene="bcp"
                     /locus_tag="ACHU2L_001201"
     CDS             9246..9716
                     /gene="bcp"
                     /locus_tag="ACHU2L_001201"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR1356146.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            9969..10586
                     /gene="hyfA"
                     /locus_tag="ACHU2L_001202"
     CDS             9969..10586
                     /gene="hyfA"
                     /locus_tag="ACHU2L_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR1356147.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10586..12603
                     /gene="hyfB"
                     /locus_tag="ACHU2L_001203"
                     /pseudo
     CDS             10586..12603
                     /gene="hyfB"
                     /locus_tag="ACHU2L_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12614..13561
                     /gene="hyfC"
                     /locus_tag="ACHU2L_001204"
     CDS             12614..13561
                     /gene="hyfC"
                     /locus_tag="ACHU2L_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR1356148.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13578..15017
                     /gene="hyfD"
                     /locus_tag="ACHU2L_001205"
     CDS             13578..15017
                     /gene="hyfD"
                     /locus_tag="ACHU2L_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR1356149.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15029..15679
                     /gene="hyfE"
                     /locus_tag="ACHU2L_001206"
     CDS             15029..15679
                     /gene="hyfE"
                     /locus_tag="ACHU2L_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR1356150.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15684..17276
                     /gene="hyfF"
                     /locus_tag="ACHU2L_001207"
     CDS             15684..17276
                     /gene="hyfF"
                     /locus_tag="ACHU2L_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR1356151.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            17266..18980
                     /gene="hyfG"
                     /locus_tag="ACHU2L_001208"
                     /pseudo
     CDS             17266..18980
                     /gene="hyfG"
                     /locus_tag="ACHU2L_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            18990..19535
                     /gene="hyfH"
                     /locus_tag="ACHU2L_001209"
     CDS             18990..19535
                     /gene="hyfH"
                     /locus_tag="ACHU2L_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR1356152.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19532..20290
                     /gene="hyfI"
                     /locus_tag="ACHU2L_001210"
     CDS             19532..20290
                     /gene="hyfI"
                     /locus_tag="ACHU2L_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR1356153.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20283..20696
                     /gene="hyfJ"
                     /locus_tag="ACHU2L_001211"
     CDS             20283..20696
                     /gene="hyfJ"
                     /locus_tag="ACHU2L_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR1356154.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20726..22738
                     /gene="hyfR"
                     /locus_tag="ACHU2L_001212"
                     /pseudo
     CDS             20726..22738
                     /gene="hyfR"
                     /locus_tag="ACHU2L_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22760..23632
                     /gene="focB"
                     /locus_tag="ACHU2L_001213"
                     /pseudo
     CDS             22760..23632
                     /gene="focB"
                     /locus_tag="ACHU2L_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23726..24787)
                     /locus_tag="ACHU2L_001214"
     CDS             complement(23726..24787)
                     /locus_tag="ACHU2L_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1356155.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            25000..26463
                     /gene="bepA"
                     /locus_tag="ACHU2L_001215"
     CDS             25000..26463
                     /gene="bepA"
                     /locus_tag="ACHU2L_001215"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR1356156.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26484..26843
                     /gene="arsC"
                     /locus_tag="ACHU2L_001216"
     CDS             26484..26843
                     /gene="arsC"
                     /locus_tag="ACHU2L_001216"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR1356157.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(26976..27677)
                     /locus_tag="ACHU2L_001217"
     CDS             complement(26976..27677)
                     /locus_tag="ACHU2L_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR1356158.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27772..29061)
                     /gene="uraA"
                     /locus_tag="ACHU2L_001218"
     CDS             complement(27772..29061)
                     /gene="uraA"
                     /locus_tag="ACHU2L_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR1356159.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29147..29773)
                     /gene="upp"
                     /locus_tag="ACHU2L_001219"
     CDS             complement(29147..29773)
                     /gene="upp"
                     /locus_tag="ACHU2L_001219"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR1356160.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
BASE COUNT         6770 a         8018 c         7815 g         7252 t
ORIGIN      
        1 aaaaaggatt cattatggtt acactttacg gcatcaaaaa ttgtgacacc attaaaaagg
       61 ctcgccgttg gctggaagcc aataacatcg actatcgttt tcatgattac cgcgtcgatg
      121 ggctggacag cgaattattg aacggtttta tcaacgaatt aggctgggaa gcgttactca
      181 acacccgtgg tacaacctgg cgtaaactgg acgaaactac ccgcaataaa atcaccgatg
      241 cggcctctgc ggcggcatta atgactgaaa tgcctgcaat tatcaaacgt ccattgctct
      301 gcgcgcctgg taagcctatg ctgctgggtt tcagtgattc cagttatcag caatttttcc
      361 atgaggtgta gtctatgtcg tgcccggtta ttgagctgac acaacagctt attcgccgcc
      421 cttccctgag tcctgatgac gcaggatgtc aggctttgtt gattgaacgt ttgcaggcga
      481 tcggttttac cgttgaacgc atggattttg ccgatacgca gaatttttgg gcatggcgtg
      541 ggcaaggtga aacgttagcc tttgccgggc ataccgacgt ggtgccgcct ggcgacgccg
      601 atcgttggat caatccgccg tttgaaccca ccattcgtga cggcatgtta ttcgggcgcg
      661 gtgcggcaga tatgaaaggc tcgctggcgg cgatggtggt agcagcagaa cgttttgtcg
      721 cacaacatcc caaccatacg gggcgactgg catttctgat cacttctgat gaagaagcca
      781 gtgcccacaa cggtacggta aaagtcgtcg aggcgttaat ggcacgtaat gagcgtctcg
      841 attactgcct ggtcggcgaa ccgtcgagta tcgaagtggt aggtgatgtg gtgaaaaatg
      901 gtcgtcgcgg atcgttaacc tgcaacctca ccattcatgg cgttcagggg catgttgcct
      961 acccacatct ggctgacaat ccggtacatc gcgcagcacc tttccttaat gaattagtgg
     1021 ctattgagtg ggatcagggc aatgaattct tcccggcgac cagtatgcag attgccaata
     1081 ttcaggcggg aacgggcagt aacaatgtta ttccgggtga actgtttgtg cagtttaact
     1141 tccgcttcag caccgaactg actgatgaga tgatcaaagc gcaggtactt gccctgcttg
     1201 aaaaacatca actgcgctat acggtggatt ggtggctttc cgggcagcca tttttgaccg
     1261 cgcgcggtaa actggtggat gcggtcgtta acgcggttga gcactataat gaaattaaac
     1321 cgcagctact gaccacaggc ggaacgtccg acgggcgctt tattgctcgc atgggggcgc
     1381 aggtggtgga actcgggccg gtcaatgcca ctattcataa aattaatgaa tgtgtgaacg
     1441 ctgccgacct gcagctactt gcccgtatgt atcaacgtat catggaacag ctcgtcgcgt
     1501 gatgagtggt tctgcaagag gaaataagca tggactggct ggctaaatat tggtggattc
     1561 tggtgattgt ctttttggta ggcgtcctgc tgaacgtgat taaagatctc aagcgcgtcg
     1621 accataagaa atttctcgcc aacaagccgg agcttccccc gcatcgtgat ttcaacgata
     1681 agtgggacga tgacgacgac tggccgaaaa aggatcaacc gaagaagtaa gctgagtgct
     1741 caaccgtagg ccggataagg cgttaacgcc gcatccggca atggtgaacg atccctgatg
     1801 cgacgctcac gcgtcttatc aggccaggta atctgtcttt tacatcattt caatcacatc
     1861 atcatcgcca ggcttaccgc cacttagcgc ttggtcgaaa taatgtttcg gaatggtata
     1921 acgcaagtga tccagggcaa attgcatact gcgattgtcg atcgcatggc ctaaatcctc
     1981 cacgatatcc agcgtcacgt caccaccggc actgattaac gcttcctgcg ccgcaactgc
     2041 atgcgccaga tcgataaccg gatcttcacc accatgaatc aagtgaattg tcgtggctgt
     2101 agacgcggtc tccggcaggc tcgcatagcg tccgttaaag gcaatgacgc gcgaagcaag
     2161 gcccggttcg gctttaatgc tctccagcgc cataatcgcg ccttgtgaga aaccaattag
     2221 cgccgtggcg ttagccccta ctccgctctg tttctgccag tagcgtaccg tttcaataaa
     2281 cgtcggcatg atggcatcca cacgcgcctg gcgattatct tccgtaatac cctgcactga
     2341 aaaccactga cgccccgccg gattaccgct cggctccgcg ccaccgacac tcaccaccag
     2401 cgcatcagga aacagcggtg caaaccagct gcctatttcc cccattgcca ccgggttatc
     2461 cccgacaccg tggaaaagca gtaacaactg ttgtgcaggt ttatccgggc tttgaacaac
     2521 aaaatggtca tgtttcatgg cgaactcctt aactgatgtc attaatttta cgccgctgac
     2581 tcacaatgac catgggagaa aattgaagga gtcagtgaaa aaattgccat tgcgttatgc
     2641 gatcgcgcag acgctcagtg cgaacctcat ttagtgcgaa cagcgcctgc gcggcctctt
     2701 cccgctggcg aaccagtaac atcttgcggc ctgaaagttt aagtgtggta cataactgcg
     2761 cgtcactggc gtttttctgt aaacgcccac gcagcgccgg taatgccagt tcactggttt
     2821 gtagcagacg caataagcaa cctaacgatg ttaatagcgg acgatgagcg aaagcaaaac
     2881 cggccagttc aagccagtcg tcgtcagaaa ggatggcatc gtttagtgga tcaacaggaa
     2941 gcatttcgcc attccactgc gcgagagctt gcgcatcgcg acgtaaacgg taatgctcac
     3001 gttcagccag ctgtttaccc gcatcactca tcggtaacag cgccatcgcc gtatagcaac
     3061 cgctgctggc ttcccgatga ttacccatcc gcaccagcac aaaaccgcag cgttgccaga
     3121 aacgccataa ctccccagtg taaccaaaac tcaccgaaag atagtcgagg tcatgcgtat
     3181 attgcaaagc tccagcaata agttgtcgcc ctgtgccttc ccgctgacga gccggatgaa
     3241 ctgctatccg gctaacccgc cttccgcgca atgtcgccgc cagtggattg ctgccgtgcg
     3301 ccgccagcga ctgggccacc agattacccc gcgggcgacg aaaacctgcc cataccgcct
     3361 gactgagttc ttgagataat ccgccctcat ccaccagcca caacgccccg gcaatctcgt
     3421 tttcgccagc cgcctgtaaa aaatgttgcc ctggtgcatc catcatccgg cgtaaatcca
     3481 gcggcgaggt ccggtagtgc gcaccagaca ataactgata aacctttaac ggcgtttctg
     3541 gctcgcttcg ccataacgtt tgttcaaatg cggaaatgac gatattgcct tgtggtgtat
     3601 gggtgaagtt ttcatcgtta aaaaccagtg cctcactaac cattttttcc agcgggcatc
     3661 cctgtgccca gcggatcggc tgttgcagtt caaaacggtg taaatgcgga aagcgagcgc
     3721 aaaatttcag caaaaaacca cgtccggtac cttcgtagcc ctgcaccgta gtggttaaca
     3781 acgttcgagg aaaacgcgat accagttgat gcaacaacgg cgcaggtatg gctgcggctt
     3841 cgtcgaccac cagccagtcg gcttgctcat cgctggctaa caaggcatcc ggtgcaataa
     3901 agcgaaactt ctcgcccgca aattgtgcca gtacatccgt tgccgctttt gcgggcgcgg
     3961 tgacaatcgc actgcccgca atacgagaaa tgagttgccc tgccaacgcc gacttaccgc
     4021 gcccacgcgc agccgttacc gctgccacgc ccggcggcat ggtcagtagc tgctgtaaga
     4081 gttgctgttg ttctggttgt ggtgcgccgg tcgcggggtg ccagtcagta cggggagtaa
     4141 aatgcgccaa cgagaacggc tggttttgcc gccagaggat agcgtcgtta tccgccgtaa
     4201 gtacgcgttt gagatgctgg acaaaatgcg gcgtcgcaat agggtcaggg caatcactcc
     4261 agcgcagcga gtcggcatca ggttggtttt cccactcttc ccatacaggg agtaacaaaa
     4321 ccagccagct tcccgctttc aacgttccgc taagtgctgc aaaggcagcg gcatcaaagc
     4381 cgtggcgggc gtcgaatacc gcatgccgga actcgcgccc aagtaaagtt tgtagcgccg
     4441 agggtgaaca gtggttttca gcatctagct gcggcgaaat ccacagccag tcgccaggta
     4501 aggcatcacg caacttaaga gcatgatcaa aacaccaacc ctcttccccg ctcaacacca
     4561 gcaagcggca gatcccttca cgtttcattt gcgctgttaa tgtgtaaagc gcagtcagtt
     4621 cagccatccc tgccccggaa attaaatgct tttaccaaaa gtattgcatt gtgccggatc
     4681 gccgctgtcg aaaccacgtt taaaccagct gtagcgttgc tgagaagtgc catgtgtgaa
     4741 actgtctggt actactcgcc cctgactttg ctgttgtaaa cggtcatcgc cgatggcctg
     4801 cgccgcattc agcgcctctt ccagatcgcc agtttccaga acgccttgct gctgcatact
     4861 atgcccccag acaccggcaa aacagtcggc ctggagttcc atacgcacag ataagcggtt
     4921 tacttccgcc tgcgtcgcgt tttgttgcag ttgacgaact ttcggctcga tgcctaacag
     4981 tttctgcaca tgatgaccga cttcatgggc gataacgtac ccctgggcaa aatcgccatc
     5041 cgcgccaagt ttgtctttca tgtcatcata gaaggagaga tcgatataaa ctgtgccatc
     5101 cgccgggcaa tagaacggcc ccataatgga ctggcccgcc ccgcagccgg tgcgcgtcat
     5161 tccacggtac atgaccagct tcggttgctg ataggtctta cccatctttt cgaactgttg
     5221 tccccaggtg tcttccgtgg ttgcctgaat caccgaggtg aattttgcgg cttcgtcttc
     5281 attagggcta attgaccgcg ttgattgttg ttgagaaacc ggctgcccgg tcatcaaccc
     5341 ggttaaatca acgccatagt agcctgcaac cagcacgact atcagtaaaa tcagcccgcc
     5401 tttaccgctc ggcagacgga aaccgggacc gcccattgac ggaccgccag agctgttgcg
     5461 cctgtcttca acattgtcac tttcacgtcg cccttgccaa cgcatagata cctcaacaat
     5521 atattcatta tagaaatgat cgtaggtggt tcagaggaag attaccacag gaaagcggga
     5581 tgaaatggcg gaggaatgag cgttcaatgc atatattcca agagcggttg tcttgccgtg
     5641 ccagctgcac ggcaagatga tgaacagaaa aatcagtcca gctgtacacc caggcggcgg
     5701 gcgacggctt cataggcttc gatcaggcca ccgaggctct ggcggaaacg gtctttgtcc
     5761 attttctcca gtgtttcttt gtcccacagg cggctgccgt ccggggagaa ctcatcgccc
     5821 agtaccactt cacctttgta cagaccaaac tccagcttga agtcgaccag aatcagacca
     5881 gcatcatcga acagtttttt cagcacgtcg ttcgctttgt aggtcagctc tttcatacgc
     5941 gccaggttct ctttgctcac ccagccaaag gtttcgcagt aagattcgtt gaccatcgga
     6001 tcgtgcatgg catcgttttt caggaacaga tcgaacagcg gcgggttcag ctcaatacct
     6061 tcttcgattc caagacgttt cactagagag ccagcagcac ggttacgcac gacacactca
     6121 accggcacca tatccagctt tttcaccaga cattcggtat cggagagcag acgctccatt
     6181 tgagtcggga tacccgcttc agccagtttg ctcataatga agtagttgaa cttgttgttc
     6241 accatacctt tgcgatcaaa ctgctcaatg cgcgcgccat cccctgctga cgtatcattg
     6301 cggaattcga gcaccaacag gtccgggttt tccgtgctgt atacggtttt cgctttacca
     6361 cgatacaact cagcttgctt ttgcatcttt atcactcctg ggtgtgaatt aacgttttaa
     6421 aatcttttgc tgtctggtgt gccggatgtt ttgtcggatg cggcgtgaac gccttatccg
     6481 aactacgtcc gacaaacaaa tttcgtgcga attaccgcta aaatcgcgtt ttcctgccga
     6541 cgcacacgtt tgcgtatcat atcagaaaaa agggccggat gattccagcc ctgtattttt
     6601 acttgctaaa cgcagcctgg aagacagcta ccagcgcgtc gttctgactc tgagtcagag
     6661 tatgaccttt cggatcgatg aactgtaggc tgctgcggtt atctaaatcg ccaacctgca
     6721 gtttatagtc accggatgcc aggcctggat cgctcgcgcc cagttcctgc cagtcgctgt
     6781 cagacagcgg cttataagtt acggccatgt tgccctgcga acgggtgctg tcggtcactt
     6841 tcatgcccac tttttccagc gccgctggca gacgttgcca aaccacattg aacggcccgc
     6901 gtacgaccag cattggtaaa ccggtgtcat cagctgcact ttgtacgtcc atagtggtgg
     6961 aggcacgatt ttgcgcagcg ttcgcggcgt cagtggcaga tttatccaga ccggcggaaa
     7021 taacgttcat catctccgtg ctgtaacgct gcatggaagc cgcgtctgca accggtttgc
     7081 ccgcctgttc caggttcagc agtttaaccg taaccgcctg ctgataaccc tgcggcttaa
     7141 cagagatttg ataacgacca cgatactgct cgtcttcgtc cagacggttc cattgtaccc
     7201 aatcggtggt cagtgtctga ccagcatcat cacgttgggt gatggtgtag tttttcgcct
     7261 gcagcacgct aaccacctgc ggccacagag tattgccacg accattttcc accagcaatg
     7321 aagcggtatc gcccgtgaac tgggtacgcg cgccagaaac cagtgccaac ggctgggctg
     7381 gtggacgaat gtccagcgcc ttaccgacag caccactacc gttggtcacc gggattgcat
     7441 aatcaccgga ggtcaccggc aaaatcattc cagccggggc atgaagctcc gcaagcggtg
     7501 ccgcttccag gtaggcttca tcaccactga cctgacgctt atagcgtgag tcagaactac
     7561 aggcagcgag taataaaaca agcgaaacac ccgcaacctt tgccaggcgc gacttttgaa
     7621 cagagtaagc catcaaatct ccctaaactt tacagcaaac cggcatgctt aagcgccgct
     7681 ctgaccgtct cacgaccact gtcggtgatt ggtgtcattg gcaggcgcag cgtatcggtc
     7741 gccacaagac ccagttcctt acatgcccat ttcaccggga ttggattggg ttcgacaaat
     7801 agtttgttgt gtaatggcat cagacgctga ttaataacgc gtgcctcggc aaaatgccct
     7861 tctgctgcca gtttgcacat ctgggccata tcacgcgctg cgacgttagc cgtaacggaa
     7921 ataactccat gaccgcccaa ttgcatgaag tccagcgcgc tcgcatcatc gccgctcagc
     7981 agaacaaaat catctgaaac cagctctttg atctggttta cacgcgttaa gttccctgtt
     8041 gcctctttga ttccgataat attttttact ttcgccagac ggcccaccgt ttccgggagc
     8101 agatcgcagc cagtacggga cggcacatta tacagaattt gcggcaggtc agtatgctca
     8161 gcgatggctt tgaaatgctg atacaaacct tcttgcgacg gacgattgta gtaaggggtt
     8221 accgtcaggc agccgacgat accactgtca ttgaagcgct gcgtcaggct aatggcttcc
     8281 gcagtagcgt tagcgccggt cccggcaatt accggaatgc gcccatcagc cagatccagc
     8341 gtcatcatca ccacatcagc atgttcgtca tgatttaagg tagcggactc gccagtggtg
     8401 ccaacagaaa cgatcgccga agtaccgctg gcgacatgat aatcaatcag ttttttcaag
     8461 ctagcccgac agacattacc tttttcatcc atcggagtaa caatcgcgac aatacttccc
     8521 gtgaacatgg gccatcctct gtgcaaacaa gtgtctcaat ggtacgtttg gtatggcatt
     8581 aaaagcaagc agacagaacc gttctgattg ttgtatgcat gattttttta tgctttcctt
     8641 aagaacaact caccccttaa aggaataacc agtttgacac tgtcatcgca acattatctg
     8701 gtgatcactg cgttgggtgc cgatcgccct ggaattgtga acaccatcac ccgtcatgtc
     8761 agtagttgcg gctgtaatat tgaagacagt cgcctggcga tgctgggaga agagttcacg
     8821 tttatcatgc tgctttccgg ttcatggaat gccatcactc tgattgaatc aacgttaccg
     8881 ttgaaaggtg ccgaactgga tcttttaatc gtgatgaagc gcacgacggc gcgtccgcgt
     8941 ccgccaatgc cagcatctgt ctgggttcag gtcgatgttg cagactcccc gcatttaatt
     9001 gaacgcttca cagcactttt cgacgcgcat catatgaaca ttgcggagct ggtgtcgcgc
     9061 acacaacctg ctgaaaatga acgggctgcg cagttgcata ttcagataac cgcccacagc
     9121 cccgcatctg cggacgcagc aaatattgag caagcgttca aagccctatg tacagaactc
     9181 aatgcacaag gcagtattaa cgtcgtcaat tattcccaac atgatgaaca ggatggagtt
     9241 aagtaatgaa tccactgaaa gccggtgata tcgcaccgaa atttagcttg ccggatcaag
     9301 acggagaaca agttaatttg accgacttcc agggacagcg tgttctggtt tatttctacc
     9361 cgaaagccat gacccccggc tgtaccgtac aggcctgcgg cttacgcgat aacatggatg
     9421 agttgaaaaa agcgggcgtt gatgtgctgg gtatcagcac cgataaaccc gaaaaactct
     9481 cccgttttgc ggaaaaagag ctgcttaact ttacgctcct gtctgatgag gaccaccagg
     9541 tgtgcgaaca attcggcgtc tggggtgaaa agtccttcat gggcaaaacc tacgatggta
     9601 ttcatcgcat cagcttcctg attgacgctg atggcaaaat cgaacatgtc tttgacgatt
     9661 tcaaaaccag caatcaccac gacgttgtgc tgaactggct gaaagaacac gcctgattac
     9721 tttgctccat tccgtgctgg ctgcgcttgc ggccagcata cctcacttct cgtgatcaag
     9781 atcacattct cgctttcccc tgcgacacgg gtgtcgaatc cattttttgc tgaacgttaa
     9841 tgaccatcat ttttgtaccg ttcagaatcc agttaataca taacttattg aatagattga
     9901 gttaatcaga atggcatcct ttatacaata tgaaatgcaa tgtttcatat cattttcaag
     9961 gagccgacat gaaccgcttt gtggtggccg aaccactgtg gtgtacagga tgtaatacct
    10021 gtctcgctgc ctgttcggac gtgcataaaa cgcaaggttt acagcaacac ccgcgcctgg
    10081 ccctggcgaa gacgtcaaca atcactgccc ctgtcgtgtg tcatcactgt gaggaagccc
    10141 cttgcctgca ggtctgcccg gtcaatgcca tctctcagag ggatgatgcg atccaactca
    10201 acgaaagcct ctgtattggc tgcaagcttt gcgccgtggt ctgcccattt ggcgcaatca
    10261 gcgcttcagg aagccgtccg gtgaatgccc atgcgcaata tgtttttcag gctgaaggct
    10321 cactcaaaga cggcgaagaa aacgtgccgc cccaacatgc gttgctgcgc tgggaaccgg
    10381 gtgtccagac cgtcgcggtg aaatgcgacc tgtgtgattt cttgccagaa ggtccggcct
    10441 gcgttcgcgc ttgcccgaat caggcgttac ggctgatcac cgacgatagc ctgcaacgtc
    10501 agatgaaaga aaaacagcgc cttgccgcaa gctggtttgc caatggcggg gagtctcccc
    10561 tttccctcac tcaggagcaa cactaatgga tgccctgcaa ttattaacct ggtcgctgat
    10621 cctctatctg tttgccagtc tggcttcgct gtttttactc ggtctggaca gactggctat
    10681 taagctttcc ggcatcacat cgctggtggg cggcgtgatt ggcatcatca gcggaattac
    10741 gcaattacat gctggtgtaa ctttagtcgc ccattttgcc accccttttg actttgccga
    10801 tttaaccctg cgaatggata gcctctcggc atttatggtg ctagttatct ccttgctggt
    10861 ggtggtttgt tcgctctatt cattgactta tatgcgcgaa tacgagggca aaggcgcggc
    10921 ggcgatgggc ttctttatga atcttttcat cgcatcgatg gttgccctgc tggtgatgga
    10981 caacgctttt tggttcatcg tgctgtttga aatgatgtcg ctgtcttcct ggtttctggt
    11041 cattgccagg caggataaaa cgtcgatcaa cgctggcatg ctctactttt ttatcgccca
    11101 cgccggatcg gtgctgatta tgatcgcctt cttgctgatg gggcgcgaaa gcggcagcct
    11161 cgattttgcc agtttcgcac gctttcactt tctccggggc tggcgtcggc ggtgtttctg
    11221 ctggcctttt tcggttttgg cgcgaaagcc gggatgatgc cgttgcacag ctggttgccg
    11281 cgcgctcacc ctgccgcacc atcgcacgct tcggcgttga tgtctggcgt aatggtcaaa
    11341 atcggtattt tcggcatcct gaaagtagcg atggatctgc tggcgcaaac gggtttgcct
    11401 ctgtggtggg gcattctggt gatggcgatc ggcgcaatct ccgcgctcct gggcgtgcta
    11461 tatgcgctgg cggaacagga tatcaaacgg ctgctggcct ggagtaccgt cgaaaacgtc
    11521 ggcattattt tgctggcagt cggtgtggcg atggacggtc tgtcactgca cgacccgctg
    11581 ctcaccgtgg ttggactgct cggcgcactg tttcatctgc tcaaccatgc gctgttcaaa
    11641 gggctgctat ttctcggcgc ggacgcgatt atttcgcgtt tgcataccca cgacatggaa
    11701 aaaatggggg cactggcgaa acggatgccg tggacagccg cagcatgcct gattggttgc
    11761 ctcgcgatat cagccattcc tccgctgaat ggttttatca gcgaatggta cacctggcag
    11821 tcgctgttct cactaagtcg tgtggaagcc gtagcgctac aacttgcggg tcctattgct
    11881 atggtaatgc tggcagtcac tggtgggctg gcagtaatgt gcttcgtcaa aatgtacggt
    11941 attactttct gtggtgcgcc gcgcagtaca cacgctgaag aggcacagga agtgccaaat
    12001 acgatgatcg tcgccatgct actgctcgcg gcactctgcg tattaattgc gcttagtgcc
    12061 agttggctgg caccgaagat aatgcatatt gcccatgcgt ttaccaatac ccctcccgtc
    12121 actgtcgcca gcggaatagc acttgtaccc ggcacgtttc atacacgggt cactccttca
    12181 ttactgttgc tgttactact ggcgatgcct ttgctgccag gcctttactg gctgtggtgt
    12241 cgttcgcgcc gcgcagcgtt tcgtcgcaca ggagatgcct gggcatgcgg ctacggctgg
    12301 gaaaacgcga tggctccgtc aggcaatggc gtgatgcagc cgctgcgtgt ggtcttctct
    12361 gcgttatttc gtctacgaca acagctcgac cctacgctga ggctaaataa aggtcttgcg
    12421 cacgtcaccg ccagggctca gagcacagaa cccttctggg atgagcgggt gatccgcccc
    12481 atcgtgagcg ccacccaacg gctggtcaaa gaaatacagc atctgcaaag cggcgacttt
    12541 cgtctctatt gcctgtatgt ggtcgccgca ctggttgtgc tgctaatcgc tattgccgtc
    12601 taaggaaatc accatgagac aaactctttg cgacggatat ctggtcattt ttgcgttagc
    12661 acaggccgtg attctgctga tgctaacccc actttttacg ggtatttccc ggcagatacg
    12721 cgcgcgtatg cactcccgcc gcgggccggg gatctggcag gattatcgcg atatccacaa
    12781 actgtttaaa cgccaggaag ttgcgccgac atcttcaggt ctgatgttcc gcctgatgcc
    12841 gtgggtatta atcagcagca tgctggtgct ggcgatggcc ttaccactat ttattaccgt
    12901 ttcccctttt gcgggcggcg gcgatctgat cacccttatc tatcttcttg ccctgtttcg
    12961 ttttttcttt gctctttccg ggctggatac cggaagtccg tttgcgggag tcggtgccag
    13021 tcgcgagttg acgctcggca ttctggtcga accaatgctt attctctcac tgctggtatt
    13081 ggcgctgata gcaggttcca cgcatatcga gatgatcagc aatacgctgg ctatgggctg
    13141 gaactcgccg ctaaccaccg tactggcgtt actggcctgt ggttttgcct gcttcattga
    13201 gatgggaaaa attccctttg atgttgctga agcagaacag gaattacagg aaggcccgct
    13261 gaccgaatat tccggtgccg ggctggcgct agcgaaatgg gggctggggc tgaaacaggt
    13321 cgtgatggca gcactgtttg tggccctgtt tctgcccttt gggcgcgcgc aagaactttc
    13381 tctcgcctgc ctgctgactt cacttgtcgt tacgctgctc aaggttttgc tgatttttgt
    13441 actggcctca atcgcagaaa acacgctggc acgcgggcgt tttttactca ttcaccatgt
    13501 gacctggctt ggcttcagcc ttgctgcgct tgcatgggtc ttttggttaa ccggtctgta
    13561 aggagcactg acggaatatg gaaaatcttg ctctgacgac gttaatgctg ccttttatcg
    13621 gcgcactggt cgtttcgttt tcgccacaac gtcgggccgc cgaatggggg gttttgttcg
    13681 ccgcgctgac cacgctgtgc atgttgtcgc tgatctccgc gttttatcag gccgataaag
    13741 ttgccgtcac gttgacgttg gtcaacgtgg gggatgtggc attatttggc ctggtcattg
    13801 atcgcgtgag tacgctgatt ctgtttgtgg tggtgtttct cggtttgctg gtcacgatct
    13861 actccacggg ttatctgacg gataaaaatc gcgaacaccc gcataacggc acgaatcgtt
    13921 attacgcatt tttgctggtg tttatcggcg cgatggcggg actggtactc tcctcgacgc
    13981 tgctcggtca gttgttgttt tttgaaatta cgggcggctg ctcctgggcg ttgatcagtt
    14041 attaccagag cgataaagcg cagcgttcag cactaaaagc gttgcttatc acccatatcg
    14101 gttcgttggg gttgtatctt gccgccgcca cgctgttttt gcagaccgga acgtttgtgc
    14161 ttagcgcgat gagcgagtta catggcgacg cacgttatct ggtttatggc ggcatcctgt
    14221 ttgccgcgtg ggggaaatcg gcccagctac cgatgcaagc gtggctaccg gacgcaatgg
    14281 aagcgccaac accgatcagc gcctatctcc acgccgcatc gatggtgaaa gtgggcgttt
    14341 acatttttgc ccgcgccatt atcgacggcg gcaatatccc gcatgtgatt ggcggcgttg
    14401 gcatggtcat gacactggtc accattcttt atggctttct gatgtatttg ccacagcagg
    14461 atatgaagcg gttgctggcc tggtcgacca tcactcaact tggctggatg ttcttcggct
    14521 tgtcgctctc catcttcggc tcgcggctgg cgctggaggg tagcatcgcc tacatcgtca
    14581 accacgcgtt cgctaaaagc ctgtttttcc ttgtagcagg tgcgctgagt tacagctgcg
    14641 gcacgcgctt gttgccgcgt ctgcgtggcg tattgcacac cctgccgttg ccaggcgtgg
    14701 gtttctgcgt ggcagcgctg gcaattaccg gtgtgccgcc gttcaacggc ttcttcagta
    14761 aattcccgct gtttgccgcc ggttttgcgt tgtcagtgga gtactggatc ctgctgcccg
    14821 ccatgattct gctgatgatt gaatcggtcg ccagtttcgc ctggtttatt cgctggtttg
    14881 gtcgcgtcgt gccgggcaaa ccgagcgagg ccgtcgccga tgccgcaccg ctgccagggt
    14941 caatgcgcct ggtgttgatt gtactgattg tgatgtcgct gatttccagc gtaatcgccg
    15001 cgacctggtt gcagtaagga gatgatgaat gaccggttct atgatcgtaa ataatctggc
    15061 gggactgatg atgctgacat cgctgtttgt gattagcgtc aaaagctatc gcctgtcatg
    15121 cggattttac gcctgccagt cgctggtgct ggtgtctatt ttcgccactc tctcgtgcct
    15181 gttcgccaca gagcaactgc tgatctggtc cgccagcgcc tttatcacca aagtgctgct
    15241 ggtaccgtta atcatgactt acgctgcacg aaatattccc cagaacatcc cggaaaaagc
    15301 gttattcggt ccggcaatga tggcactgct cgcggcgtta attgtcctgc tttgcgcatt
    15361 tgtcgttcag cccgtgaagc taccgatggc taccgggctg aaaccggcgc tggcggtagc
    15421 gttaggtcat tttctgcttg gcctgctgtg cattgtcagc caacgcaata tcctgcggca
    15481 aatttttggt tactgcctga tggaaaacgg ctcccatctg gtgctggcgc ttcttgcctg
    15541 gcgagcaccg gaactggtgg aaataggtat cgctaccgac gccatcttcg ccgtcattgt
    15601 gatggtgtta ctggcaagaa aaatatggcg tacccacggc acgctggacg tgaacaactt
    15661 gaccgcgctg aagggataat gagatgagtt attctgtgat gttcgcttta ctcctgctca
    15721 cgccgctgct tttttcgctg ctctgttttg cctgccggaa acgggggctc tctgcgactt
    15781 gtacggtgac tgtattacat agcttaggga tcacactgct gctgattcta gcactctggg
    15841 tggtccaaac tgccgctgat gcaggagaaa tattcgctgc gggactgtgg cttcatattg
    15901 atggtctggg cggtttgttc ctcgccattc ttggtgtgat tggctttctc accggtgttt
    15961 actcgattgg ctacatgcgt catgaagtgg cacacggcga gctttcaccc gttacactgt
    16021 gcgattacta cggtttcttc catctgtttt tgttcaccat gctgctggtt gttaccagca
    16081 ataacctgat tgtgatgtgg gcggcgatcg aagccaccac cttaagctcg gcgtttctgg
    16141 taggcattta cggtcagcgt tcatcgctgg aatcagcatg gaagtacatc attatttgta
    16201 ctgttggtgt cgcttttggt ctgttcggta ccgtgctggt atacgccaac gccgccagcg
    16261 ttatgccgca ggcagaaatg gcgatattct ggagcgaggt tcttaagcaa tcgtccttgc
    16321 ttgacccaac attaatgctg ttggcctttg tgtttgtgct aattggcttt ggtaccaaaa
    16381 ccgggctatt tcccatgcac gcctggctgc cggatgctca cagtgaagcg ccgagtccgg
    16441 tcagcgccct gctctccgcc gtattgctga actgcgcgct gttggtgctg attcgctatt
    16501 acatcattat ttgccaggcc atcggcagcg atttccccaa ccggttgttg ctcatcttcg
    16561 gcatgttgtc ggttgccgtg gcggcatttt tcattctggt acagcgggac attaagcgtc
    16621 tgctggcgta ctccagcgtg gagaacatgg ggctggtcgc ggtggcgcta ggcattggcg
    16681 ggccgctggg aatttttgcc gcgctgctgc acaccttaaa ccacagtctg gcaaaaacgc
    16741 tgctgttctg cggttccggc aatgtactgc tcaagtacgg cacgcgcgat ctcaacgtcg
    16801 tctgtgggat gctcaaaatc atgccattta ccgccgtgct gtttggcggc ggtgcgctgg
    16861 cgctggcgct ggcgctggca gggatgccgc ccttcaacat ttttcttagc gaatttatga
    16921 ccgttaccgc aggactggca cgtaatcacc tgctgattat cgtcctgctg ttattgctgt
    16981 taacgctggt gctggcgggc ctggtacgga tggctgcgcg ggtgttaatg gcgaaaccgc
    17041 cgcaggccgt taaccggggt gatctcggct ggttgaccac ctcgccaatg gtgattctgc
    17101 tggtcatgat gctggcgatg ggaacgcata ttccacaacc tgtcatcagg atcctggcgg
    17161 gcgcttccac tatagtcctc tcagggacgc acgacctgcc tgcacaacgt agcacctggc
    17221 atgatttttt gccttcaggc accgcatctg tttcggagaa acacagtgaa cgttaattca
    17281 tcgtcaaatc gtggcgaagc gattctcgcc gccctgaaaa cgcagttccc cggcgcggtg
    17341 ctggatgaag agcgacaaac gcctgaacag gtcaccatta cggtgaaaat caatctgctg
    17401 cctgacgttg tacattatct ttattatcaa catgatggct ggcttccagt cctgtttggc
    17461 aacgacgagc ggacacttaa cggtcattac gcggtttatt atgccctttc aatggaaggg
    17521 gccgaaaaat gctggatcgt ggtgaaggca ctggtcgatg ccgacagtcg ggagtttccg
    17581 tcagtcacac cgcgcgtccc tgccgcggtc tggggcgagc gagaaattcg cgatatgtac
    17641 gggctgattc cggttggcct gccggatcag cgtcgcctgg tgttgcccga tgactggccg
    17701 gaagatatgc atccgctgcg caaagatgcg atggattatc gactgcgccc tgaaccgacg
    17761 acagattccg aaacgtatgc gtttatcaat gagggcaaca gcgatgcgcg ggtgatccct
    17821 gtcggcccgc tgcatatcac ctcctatgaa ccgggtcact tccgcttgtt tgtggatggc
    17881 gagcaaattg tcgatgctga ttaccgcctg ttttatgtcc atcgcggcat ggagaaactg
    17941 gcagaaacgc ggatgggcta caacgaagtg accttcttat cggaccgcgt gtgtgggatt
    18001 tgcggttttg cccacagtgt ggcctatacc aactcggttg aaaatgcact ggggattgag
    18061 gtgccgcaac gagcacatac tattcgctcg attctgctgg aagtcgagcg gctacacagt
    18121 catttgctca accttggcct ctcctgccat tttgttggtt ttgataccgg ctttatgcaa
    18181 tttttccgcg tgcgggaaaa gtcgatgacg atggcggaat tgctgaccgg gtcgcgtaaa
    18241 acctacggtc tgaatctgat tggtggtgtt cgccgcgata ttctcaaaga gcaacgtctg
    18301 caaacgctga aactggtgcg cgagatgcgc gccgacgtgt cggagctggt agagatgctg
    18361 cttgctacgc cgaatatgga acaacgcact cagggcattg gcattctcga ccgacaaatc
    18421 gcccgtgatt atagccctgt agggccgctg atccgcgcag tggttttgcc cgtgatttgc
    18481 gctttgatca cccctacgcc gactacggca atattccaaa aacactgttt acctttaccg
    18541 gcggcgatgt tttctcccgc gtgatggtcc gtgtcaaaga gacgtttgat tcgctggcaa
    18601 tgctggaatt tgccctcgac aacatgccgg ataccccact gctgaccgaa ggctttagct
    18661 ataaacctca cgcattcgcg ctgggctttg ttgaagcgcc acgcggtgaa gacgtgcact
    18721 ggagcatgct cggtgataac caaaaattgt tccgctggcg ctgtcgtgcc gccacctacg
    18781 ccaactggcc ggtgttgcgt tacatgctgc gcggcaatac cgtttctgac gcaccgctga
    18841 ttatcggtag ccttgatccc tgctactcct gtaccgaccg tgtgacgctg gtagatgtgc
    18901 gcaagcgcca gtcaaaaacc gtgctgtata aagagatcga acgctacggc attgatcgta
    18961 accgttcgcc gctgaagtaa ggacagaaga tgctgaagtt actgaaaact attatgcgcg
    19021 ccggaaccgc gacggtgaaa tatcccttcg cgccactgga ggtcagccca ggctttcgcg
    19081 ggaaaccgga cctgatgccc agccaatgta ttgcctgcgg tgcctgcgcc tgtgcttgtc
    19141 cggcaaatgc gctgactatc cagaccgacg accagcaaaa ttcgcgcacc tggcagctct
    19201 atctggggcg ttgtatttac tgcggacgtt gtgaagaagt gtgcccgacc agagccatcc
    19261 agcttaccaa taactttgaa ctgaccgtca cccataaagc cgatctctat acccacgcga
    19321 cgttccatct acaacgttgc agccgttgcg aacgcccgtt tgccccgcaa aaaaccgtcg
    19381 cactggctgc tgaattgttg gcacagcaac aaaatgctcc acaaaaccgc gaaatgttat
    19441 gggcgcaagc gagcgtctgc ccggagtgca aacaacgcgc aacgctgatc aacgacgata
    19501 cagatgtacc gctggtggct aaggagcagc tatgagtcca gtgcttacgc aatatgtcag
    19561 ccagcccatc acgctggacg agcaaacgca aaagatgaag cggcatttgc tacaggatat
    19621 ccgtcgctcg gcttacgttt atcgcatcga ttgcggcggc tgcaacgcct gtgaaatcga
    19681 aatttttgct gccatcaccc ccgtattcga cgcagaacgt tttggcatta aggttgtttc
    19741 atcaccgcgt cacgccgata ttttgttatt tactggcgca gtcacccggg cgatgcgtat
    19801 gcctgcactt cgggcgtatg agtctgcccc cgatcataaa atctgtgttt cctacggcgc
    19861 gtgcggtgtc ggcggcggta ttttccacga tctctacagc gtctggggcg gcagcgacac
    19921 cattgtcccc attgatgttt ggatccccgg ctgcccgcca acaccggccg ccaccattca
    19981 cggtttcgcc gtggcgctcg gtttgctgca acagaagatt cacgcggtgg attatcgcga
    20041 tcccaccggg gtgactatgc aaccgttgtg gccgcagatc ccgccatcac agcgtatcgc
    20101 cattgagcga gaagcgcggc ggctggcggg ctatcgtcag gggcgagaaa tttgcgatcg
    20161 gctcctgcgc catttaagcg acgatcctac aggaaatcgg gttaacacct ggttgcgcga
    20221 tgccgacgat ccacgtctca atagtatcgt tcagcaactc tttcgcgtac tccgggggtt
    20281 acatgactga agagtgcggg gaaattgttt tctggacgct gcgaaaaaag tttgtcgcca
    20341 gtagcgacga gatgccggaa cacagctctc aggtaatgta ttactcgctg gctatcggtc
    20401 atcacgttgg cgtgattgat tgtctgaatg tcgccttccg ctgcccactg acggaatacg
    20461 aagattggct tgcactggtc gaagaggagc aagcccgacg taagatgctg ggggtgatga
    20521 cttttggtga gattgttatt gacgccagcc acaccgccct gttgacccgg gcattcgcgc
    20581 cactggcaga tgacgcgacg tctgtgtggc aagcgcgtag cattcaattc attcatctgt
    20641 tggatgaaat tgtgctggaa ccggctatct atctgatggc cagaaaaatt gcgtgagaag
    20701 gatttctcat taataaggac tgttgatggc tatgtcagac gaagcgatgt ttgccccgcc
    20761 gcaaggaata acaattgaag cggtaaacgg aatgctcgcg gagcggttag cgcagaaaca
    20821 cggcaaggcg tctttattac gcgccttcat cccgctgccg ccgccgttca gcccggtaca
    20881 acttattgaa ctgcatgttc tcaaaagcaa cttctattac cgctaccatg atgatggcag
    20941 cgatgtgacg gcaacaacag agtatcaggg cgagatggtc gattattcgc gttacgccgt
    21001 ccttctcggc agtagtggaa tggcggagct acgctttatt cgtacccacg gcagccgttt
    21061 tactccccag gattgcacac tgtttaactg gctggcacgc attatcaccc cggttctgca
    21121 atcatggctc aatgatgaag cacagcaggt ggcgctgcgt ttgctggaga aagatcgcga
    21181 tcatcatcgg gtactggtgg atatcactaa tgcagtgctg tcacatcttg atctcgacga
    21241 tctgatcgct gacgtcgctc gtgagatcca tcattttttc ggtctggctt cagtcagtat
    21301 ggtactgggc gatcatcgaa agaacgagaa gttcagcctg tggtgcagcg atctttctgc
    21361 ctcacattgt gcgtgtctgc cacacaatat gcctggcgac agtgtattgc tgacacaaac
    21421 gctacaaacc cgacaaccga ccttgacgca ccgtgcagat gatctgtttc tctggcaacg
    21481 cgacccgtta ttactcttac ttgcatctaa cggctgcgaa tctgcgctcc ttataccgct
    21541 tacctttggc aaccatacac cgggtgcatt gttgctggcg catacctctt ccactctctt
    21601 tagtgaggaa aactgccagc tactacaaca catagccgat cgcatcgcta ttgccgttgg
    21661 caatgccgat gcctgccgta gaatgaccga tttgcaggaa agtttgcagc aagaaaacca
    21721 ccagcttagc gagcagctcc tttcgaatct gggcgtcggt gacattatct atcaaagcca
    21781 ggcaatggaa gacctgctcc agcaggtaga tattgtggcg aagagcgaca gtacggtgtt
    21841 gatttgcggt gaaaccggaa ccggcaaaga ggtgatcgcc agagcgatcc atcaacttag
    21901 cccgcgacac gacaagccgc tggtcaaaat caactgcgct gccatccccg ccagtcttct
    21961 ggaaagtgag ttattcggtc atgacaaagg ggcgtttact ggtgcgatta atacccatcg
    22021 tggtcgtttt gaaattgccg atggcggcac gttgtttctc gatgaaattg gcgatctgcc
    22081 gttagaactt cagcctaaac tgctgcgcgt attgcaggaa cgggagattg agcgtctcgg
    22141 cgggagtaga acgatcccgg taaatgtcag agtcattgcc gccaccaacc gtgatttgtg
    22201 gcaaatggtt gaagatcgcc agtttcgcag cgatctcttt tatcgcctga atgtcttccc
    22261 actggaattg cctccgctgc gcgaccgtcc ggaagatatc cctcttttag caaagcattt
    22321 cacgcaaaaa atggcgcgcc atatgaatcg ttcaattgac gccatcccga ccgaggcgct
    22381 acgccagttg atgtcgtggg actggccggg caacgtgcgc gagctggaaa acgtgattga
    22441 gcgagcggta ctgctgactc gcggtaacag tctgaattta catcttaatg tccgacaaag
    22501 ccgtttactg ccgacgctaa atgaagattc agcgcttcgc agttcaatgg cgtagttgct
    22561 gcacccgacg acgccagaga atgacgaaga agaacgtcag cgcattgttc aggtattgcg
    22621 agaaaccaat ggcattgttg ccgggccccg tggcgcggcg acacgattag ggatgaagcg
    22681 caccacgctg ctgtcacgaa tgcagcgtct gggaatctcg gttcgcgagg tgttgtaatc
    22741 tgcttttgca ggagtatgca tgagaaacaa actctctttc gacttgcagt tgagcgccag
    22801 aaaagcggca atcgctgaac ggattgccgc ccataaaatt gcccgcagta aagtgtcggt
    22861 ctttttaatg gcgatgtccg ctggcgtgtt tatggcgatg tccgctggcg tgtttatggc
    22921 gatcggattt actttttacc tttccgttat cgccgatgcc ccgtcttcat aggcattaac
    22981 ccatctggtg ggcggccttt gctttacact tggctttatt ttgctggcgg tttgcggcac
    23041 cagcctgttc acctcgtcgg taatgacggt aatggcaaaa agtcggggcg ttattagttg
    23101 gcgaacttgg ctgattaacg cacttctggt ggcctgcggt aatctggcag gtattgcctg
    23161 tttcagtttg ttaatctggt tttccgggct ggtgatgagt gaaaacgcga tgtggggagt
    23221 cgcgatttta cactgcgccg agggcaaaat gcatcataca tttactgaat ctgtcagcct
    23281 cggcattatg tgcaatctga tggtttgcct ggcgctgtgg atgagttatt gcggacgttc
    23341 gttatgcgac aaaatcgtcg ccatgatttt gtccatcacc ctgtttgtcg ccagtggctt
    23401 tgagcactgt atcgccaatt tgtttgtgat tccgttcgcc attgccattc gccatttcgc
    23461 ccctacctcc ttctggcaac tggcgcacag tagcgcagac aattttccgg cactgacggt
    23521 cagccatttt attaccgcca atctgctccc ggtgatgctg ggtaatatta tcggcggtgc
    23581 agtgctggtg agtatttgtt atcgggctat ttatttacgt caggaatcct gaaaaatcag
    23641 cccggcgaaa cagttcgtcg ggctatttat ttacgtcagg aatcctgaaa aatcagcccg
    23701 gcgaaacagt tcgtcgggct gaagattatt cttgcgcgat ttgcccatcg ggccaggcgt
    23761 ggatcacggc tttgatcagc gtcgccaatg gaatggcgaa gaatacgccc cagaatcccc
    23821 acaaaccacc gaagatcacc accgataaaa taatcaccag cggatgcagg ttaaccgctt
    23881 cggagaacaa caccggtact agcaggttgc cgtccagcgc ctgaataatc agatacaccg
    23941 cgaagcagct ccagaattca gtacctgcgc caaactggaa tagcgccacg ccaaccaccg
    24001 gaatggtcac cacaaatgcg ccaatgtacg gaataagaac cgagaagccg accagcactg
    24061 ccagcagcag cgaatagttc agcccgaaga gcaagaaccc cagccaggtg gcgatcccca
    24121 ccacgatcat ctccagcact ttgccgcgga tatagttggt gatttgttga ttcatctcct
    24181 tccacacctg tcctgccagc ccacggttac gcggcagcac ccggcgaacg gcgttcagca
    24241 tctgctcttt gtctttcagc aggaagaaga ccatcaatgg caccagcacc agatagacgg
    24301 ctatggtcag caaaccgacc agcgaggcga gggaaatttt caccaccgaa tcgcccatgg
    24361 tcagcatccg actgcgcata ttttcggcca ttgcatcaat aatgcccgcg tccattaaca
    24421 ccggatagcg gcgcggcaac gtggcggcaa agtcagaaag cttattgagc atccccggca
    24481 tatcgcggat taagtagatg ccctgttgcc aggcgatagg cagtaccacg aacgccatca
    24541 gtagcaatat accgacaaaa accaccaata caatcgacgt cgcccagcgg cgggagcagc
    24601 caatagattg caggcgcacg gttggccatt ccagcaaata agccagcaca atagccacca
    24661 gcaacggagc aagcaggcca ctaaagaaaa agataatgcc aaatccggca actaaaataa
    24721 ccagcaaggc aatcgcttcc gggtcgctaa aacggcggcg ataccattgc atcaacattt
    24781 cgagcataaa gaccttccct gaacctcaag agcgggattg cgatccgcaa ttgtatcgaa
    24841 atgtcacaaa aaagacttcg ctttttatga cggattcagg aaactgaaaa gtcatttgag
    24901 tgggctaatc ttcgccgtta cactcaaagg cggcgaggtg ggaacgatat ttcacagtat
    24961 cggtcaaatg actatcttca gaaatacagg atagaggtta tgttcaggca gttgaaaaaa
    25021 aacctggttg caaccctcat tgctgctatg accattggtc aggtagcccc ggcgtttgcc
    25081 gacagcgcag acaccttgcc ggatatggga acctccgcag gaagcacgct ttccattggt
    25141 caggaaatgc agatgggcga ctattatgtc cgccagctac gcggcagcgc gccgttaatt
    25201 aatgacccgc tgttaacgca atatattaat tcgctgggga tgcgtctggt ttcgcatgcc
    25261 aattcggtta agacaccgtt tcattttttt ctgatcaaca acgacgaaat taacgccttt
    25321 gctttctttg gcggcaacgt ggtgctgcac tctgccctgt tccgttattc cgataacgaa
    25381 agtcaactgg cttcagttat ggcgcacgaa atctcccacg tcacccaacg tcacctggcg
    25441 cgagcgatgg aagatcaaca acgcagcgcg ccgctgacct gggtcggcgc gttaggttct
    25501 attttactgg cgatggccag tccgcaggcg gggatggcgg cgctgaccgg tacactggcg
    25561 ggaacgcgcc aggggatgat cagtttcacc cagcaaaatg aacaggaagc ggaccgcatt
    25621 ggtattcagg tgctgcaacg ctcgggattc gatccacagg cgatgccgac cttcctggaa
    25681 aaattgctcg atcaggcgcg ttactcctcg cgcccgccgg aaattttatt gactcacccg
    25741 ttgccggaaa gtcgtctggc agatgcccgc aaccgtgcta atcagatgcg cccgatggtg
    25801 gtgcagtcgt cggaagattt ctatctggcg aaagcgcgca cactggggat gtataattcc
    25861 ggacgtaacc agctcaccag tgatttgctg gatgaatggg cgaaaggaaa cgttcgtcag
    25921 caacgagcgg cgcaatatgg tcgtgcttta caggcgatgg aagccaataa atacgacgag
    25981 gcgcgtaaaa cgctgcaacc gttactggcg gcagaacctg gcaacgcatg gtatctcgat
    26041 ctggctaccg atatcgatct tgggcaaaac aaagccaatg aggcaatcaa tcgcctgaaa
    26101 aatgcccgcg atttacgcac caatccggtg ttgcagctca acctggcgaa cgcttatctg
    26161 caaggcggtc aaccacaaga agcggccaat attctgaatc gctacacctt taataataaa
    26221 gatgacagca acggctggga tttgctggca caggcggaag ccgcgctaaa taaccgcgat
    26281 caggagctgg ctgcgcgagc agaaggttat gcgctcgccg gacgactcga tcaggccatt
    26341 tcgctgttga gtagcgccag ttcgcaggtg aaattaggca gcctgcaaca agcgcgttac
    26401 gatgcgcgca tcgaccagtt gcgccagctg cagaaacgct ttaagcctta taccaagatg
    26461 taattcgcat taaaggaaga acaatgacca aacaggtaaa aatctaccat aacccacgct
    26521 gttcaaagag ccgggaaacg ctgaacctgc tgaaagaaaa cggcgtggag ccagaagtgg
    26581 tgctctacct tgagacaccc gccgatgcgg caacgctgcg cgatttgctg aaaatgctgg
    26641 ggatgaatag cgcccgtgaa ttgatgcgcc agaaagagga tctttataaa gaactaaacc
    26701 tggctgacag ttcgcttagc gaagaggcgc tgattcaggc aatggtggat aatccgaagc
    26761 tgatggagcg cccgattatg gtcgcgaatg gcaaagcgcg gattggtcgt ccaccggagc
    26821 aggtactgga aatcgtgggt taagatgtgc tccatcgcta gttgaagcac attgccggat
    26881 gcgacgcttg ccacgtctta tccggcctac gaatactatg gattaacttc gcgtaggtca
    26941 ggcgttcgcg ccgcatccga caataaacac cttatctaca acttcagaat ttctttcaca
    27001 aacggaatgg tcagcttacg ttgcgcggta atcgacgcac gatccaactg atccaacgtc
    27061 ataaatagcg tgcgcatttc tctgtcgagc cgcttcagca agaaacgccc cacatcttcc
    27121 ggcagttcaa aaccacgcaa acgcgcgcgt aactgtagcg cctgcaactt atcttcatca
    27181 gaaagtggct gcaatttgta gatctgcccc cagtcgagac gcgacgcgag atccggtaat
    27241 cccagattca actgccgcgg tggacgatcg ccggtgatca acaaccgtgt tttgcccgat
    27301 tccagaattc gattgtagag atcgaaaatc gccatctccc acaactcatc gcctgcaata
    27361 cactcaatgt tgtcgataca gaccagcgac aaatgctcca taccgtcgag cacttccgga
    27421 acaaaccagg tgcgtttatc cagcgggaca tagcccaccg catcgccacg ctgcgacaat
    27481 tccgcgcaag ccgcgtgcag cagatggctg cgccccgcgc cttcgcgtgc ccagagatag
    27541 atgtaaccgc tatgttcctg acgcagcacg ttttgcagcg cggccagtaa agaggagtta
    27601 tcccccggcc agaaacttgc aaaggtttcg tcgtcaggaa gataaagtgg caaagagagc
    27661 tgtgccggtg tgttcagaga tacctcaacc aggatttcac aaaatcgcga gaagtttacc
    27721 acagaatccc atgatgtttg aaccgggcag caacactgcc cggtcggtac attatttgtc
    27781 tgttatatcc gcgtcttctg cgtccagcac cacttcttca gggcgcagca cactgatcag
    27841 tttgaaaatc aaacttaagc cgataccgac gatggtcgcc agcgccatac cttttaactc
    27901 cgccgcgccg atgtttacct tcgcgccact gacgccgatg atcaaaatca cggaagtcag
    27961 gatcaggttc tgtgctttgt tgtaatccac tttcgattcg atcaaaacac gaataccgga
    28021 agcaccaatg acaccataaa gcagcagcga aacgccgccc atcaccggca atgggatcat
    28081 ctggatagcg gcagccagtt taccgacgca ggaaagcagg atagcgaaaa tcgccgcccc
    28141 gccgataacc caggtactgt aaacacgggt gatcgccatc acgccaatgt tttctccgta
    28201 agtggtattt ggcgtagagc caaagaagcc ggagatcacg gtcgacaggc cgttagcaaa
    28261 catcgaacgg tgcagacctg gatcgcgcag cagatctttt ttgacgatat tagccgttac
    28321 taccaggtgc cctacgtgtt cggcaataac cactaacgcc gctggcagaa tagtcagaat
    28381 ggcaaaccac tcgaagcgcg gcgtatagag ggttggcagt gcaaaccagt gagcattaat
    28441 aatcggcgtg gtatcgacaa ttcccattgc gaaagagagc gcgtacccca ccagcacgcc
    28501 aattaaaatc gggataattg ccaggaaacc acgaaacagc acggaaccta aaaccgtgac
    28561 cgccagggtg gtaatagaga tgatgatggt tttggagttt ggcgtttgcc cttcagccgg
    28621 gagtaaaccc gccataccgg cagctacgcc cgccagctcc agaccgatga cggcaacgat
    28681 tgcgcccatt gccgcaggtg gaaacagcac gtccaaccag ccggtccccg ctttcttcac
    28741 gataaaagaa accaggcaga acagcacgcc gcacataata aagccgccca gcgcgacttc
    28801 ataccctaac ggcaacagta acaataccgg tgaaataaag gcaaagctgg aaccaagata
    28861 agccggaatt ttccctttac agatgaagag atacagcagc gttccaatac cgttaaataa
    28921 cagtacagtc gccgggttaa tatgaaataa gacgggcacc aggacggttg caccaaacat
    28981 ggcgaacaaa tgttgcaaac taagcgggat tgtctgtaaa agtggcggtc tttcactcac
    29041 cccgatagca cggcgcgtca tagtattatc ctctgtatta tgtgttatag gcgctttact
    29101 caaaaaaaag ccgactctta aagtcggctt taattatttt tattctttat ttcgtaccaa
    29161 agattttgtc accggcatcg ccgaggcccg gaataatgta tccgtgctcg ttcagtccct
    29221 ggtcaatcga tgcagtatac agttcgacgt ccgggtgcgc tttttccagc gcagcgatac
    29281 cttctggcgc agcgaccagc accagaactt tgatgctgct gcagcccgct tttttcagca
    29341 ggtcgatggt cgcgataacg gaaccaccgg ttgccagcat tgggtcaacg atcagcgcca
    29401 tacgctcatc gatgttagaa accagtttct ggaagtacgg taccggctcc agcgtttctt
    29461 cattacggta cataccgaca acgctgatgc gcgcgctcgg aacgttttcc agcacaccgt
    29521 ccatcatacc aagacccgca cgcagaattg gcacaacggt aattttctta cctttgatct
    29581 ggtcgatttc taccgggccg ttccagcctt cgatagttac tttttccgtt tcgaggtcgg
    29641 cggtcgcttc gtaagtcagc aggctaccca cttcggaagc gagttcgcga aagcgcttgg
    29701 tgctgatatc ttgctcacgc atcagtccca gcttgtgttt gacgagtggg tgtttgactt
    29761 ccacgatctt catactcttt ctcctttgag gggcagccac aaaaaaatcg acggattata
    29821 cctcctttct tcagggcggc aatattattt tcgtt
//
LOCUS       DAZPEU010000029        29367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPEU010000029 DAZPEU010000000
VERSION     DAZPEU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.029"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHU2L_001220"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHU2L_001220"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR1356161.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHU2L_001221"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHU2L_001221"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR1356162.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHU2L_001222"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHU2L_001222"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR1356163.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHU2L_001223"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHU2L_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR1356164.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHU2L_001224"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHU2L_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR1356165.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHU2L_001225"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHU2L_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR1356166.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHU2L_001226"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHU2L_001226"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR1356167.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHU2L_001227"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHU2L_001227"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR1356168.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHU2L_001228"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHU2L_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR1356169.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHU2L_001229"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHU2L_001229"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR1356170.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHU2L_001230"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHU2L_001230"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR1356171.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHU2L_001231"
     CDS             8212..9075
                     /locus_tag="ACHU2L_001231"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR1356172.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHU2L_001232"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHU2L_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR1356173.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHU2L_001233"
     CDS             10411..11028
                     /locus_tag="ACHU2L_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR1356174.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHU2L_001234"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHU2L_001234"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR1356175.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHU2L_001235"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHU2L_001235"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR1356176.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHU2L_001236"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHU2L_001236"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR1356177.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHU2L_001237"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHU2L_001237"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR1356178.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHU2L_001238"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHU2L_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR1356179.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHU2L_001239"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHU2L_001239"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR1356180.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHU2L_001240"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHU2L_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR1356181.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACHU2L_001241"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACHU2L_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR1356182.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACHU2L_001242"
     CDS             21859..23568
                     /locus_tag="ACHU2L_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1356183.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHU2L_001243"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHU2L_001243"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1356184.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACHU2L_001244"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACHU2L_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACHU2L_001245"
     tRNA            27625..27719
                     /locus_tag="ACHU2L_001245"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACHU2L_001246"
     CDS             27991..29202
                     /locus_tag="ACHU2L_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1356185.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT         7272 a         7851 c         7354 g         6890 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaata
//
LOCUS       DAZPEU010000030        28914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPEU010000030 DAZPEU010000000
VERSION     DAZPEU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.030"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHU2L_001247"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHU2L_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR1356186.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACHU2L_001248"
     CDS             complement(2138..2584)
                     /locus_tag="ACHU2L_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR1356187.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACHU2L_001249"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACHU2L_001249"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR1356188.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHU2L_001250"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHU2L_001250"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR1356189.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACHU2L_001251"
     CDS             complement(5347..6009)
                     /locus_tag="ACHU2L_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR1356190.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACHU2L_001252"
     CDS             6182..6595
                     /locus_tag="ACHU2L_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR1356191.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHU2L_001253"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHU2L_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR1356192.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHU2L_001254"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHU2L_001254"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR1356193.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACHU2L_001255"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACHU2L_001255"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR1356194.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHU2L_001256"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHU2L_001256"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR1356195.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHU2L_001257"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHU2L_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR1356196.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACHU2L_001258"
     tRNA            complement(9861..9948)
                     /locus_tag="ACHU2L_001258"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHU2L_001259"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHU2L_001259"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR1356197.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHU2L_001260"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHU2L_001260"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR1356198.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHU2L_001261"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHU2L_001261"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR1356199.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHU2L_001262"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHU2L_001262"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR1356200.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHU2L_001263"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHU2L_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR1356201.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACHU2L_001264"
     CDS             14984..15841
                     /locus_tag="ACHU2L_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR1356202.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACHU2L_001265"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACHU2L_001265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR1356203.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACHU2L_001266"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACHU2L_001266"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1356204.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHU2L_001267"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHU2L_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR1356205.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACHU2L_001268"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACHU2L_001268"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR1356206.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHU2L_001269"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHU2L_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHU2L_001270"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHU2L_001270"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR1356207.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACHU2L_001271"
     CDS             complement(22884..24023)
                     /locus_tag="ACHU2L_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR1356208.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACHU2L_001272"
     CDS             complement(24069..26165)
                     /locus_tag="ACHU2L_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1356209.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACHU2L_001273"
     CDS             complement(26165..26911)
                     /locus_tag="ACHU2L_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1356210.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHU2L_001274"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHU2L_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR1356211.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACHU2L_001275"
     CDS             complement(27659..27964)
                     /locus_tag="ACHU2L_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356212.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHU2L_001276"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHU2L_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR1356213.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7002 a         7320 c         7601 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aagccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAZPEU010000031        28221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPEU010000031 DAZPEU010000000
VERSION     DAZPEU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.031"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHU2L_001277"
     CDS             complement(<1..209)
                     /locus_tag="ACHU2L_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1356214.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHU2L_001278"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHU2L_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR1356215.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHU2L_001279"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHU2L_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR1356216.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHU2L_001280"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHU2L_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR1356217.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHU2L_001281"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHU2L_001281"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR1356218.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHU2L_001282"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHU2L_001282"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR1356219.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHU2L_001283"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHU2L_001283"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR1356220.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHU2L_001284"
     CDS             complement(4598..5320)
                     /locus_tag="ACHU2L_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1356221.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHU2L_001285"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHU2L_001285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1356222.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHU2L_001286"
     CDS             6520..6738
                     /locus_tag="ACHU2L_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR1356223.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHU2L_001287"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHU2L_001287"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1356224.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHU2L_001288"
     CDS             complement(7472..7672)
                     /locus_tag="ACHU2L_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR1356225.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHU2L_001289"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHU2L_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHU2L_001290"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHU2L_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR1356226.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACHU2L_001291"
     CDS             10101..12014
                     /locus_tag="ACHU2L_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1356227.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACHU2L_001292"
     CDS             12014..13036
                     /locus_tag="ACHU2L_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1356228.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACHU2L_001293"
     CDS             13030..13248
                     /locus_tag="ACHU2L_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR1356229.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACHU2L_001294"
     CDS             13302..14171
                     /locus_tag="ACHU2L_001294"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR1356230.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACHU2L_001295"
     CDS             complement(14226..14630)
                     /locus_tag="ACHU2L_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR1356231.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACHU2L_001296"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACHU2L_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR1356232.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACHU2L_001297"
     CDS             15615..17705
                     /locus_tag="ACHU2L_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR1356233.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHU2L_001298"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHU2L_001298"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR1356234.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHU2L_001299"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHU2L_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR1356235.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACHU2L_001300"
     CDS             complement(19673..20275)
                     /locus_tag="ACHU2L_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR1356236.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACHU2L_001301"
     CDS             complement(20265..20432)
                     /locus_tag="ACHU2L_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR1356237.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHU2L_001302"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHU2L_001302"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR1356238.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHU2L_001303"
                     /pseudo
     CDS             21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHU2L_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
     gene            22831..25374
                     /gene="nirB"
                     /locus_tag="ACHU2L_001304"
     CDS             22831..25374
                     /gene="nirB"
                     /locus_tag="ACHU2L_001304"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR1356239.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25371..25697
                     /gene="nirD"
                     /locus_tag="ACHU2L_001305"
     CDS             25371..25697
                     /gene="nirD"
                     /locus_tag="ACHU2L_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR1356240.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25823..26629
                     /gene="nirC"
                     /locus_tag="ACHU2L_001306"
     CDS             25823..26629
                     /gene="nirC"
                     /locus_tag="ACHU2L_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR1356241.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26648..28021
                     /gene="cysG"
                     /locus_tag="ACHU2L_001307"
     CDS             26648..28021
                     /gene="cysG"
                     /locus_tag="ACHU2L_001307"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR1356242.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6852 a         7543 c         7105 g         6721 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgctaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa gaaaagtggg ggatcggcgt actgtttctc
    22021 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22081 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22141 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22201 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22261 acaccagtac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22321 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22381 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcacgatt
    22441 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22501 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22561 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22621 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22681 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22741 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22801 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22861 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22921 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctca
    22981 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23041 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23101 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23161 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23221 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23281 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23341 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23401 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23461 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23521 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23581 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23641 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23701 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23761 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23821 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23881 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    23941 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24001 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24061 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24121 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24181 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24241 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24301 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24361 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24421 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24481 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24541 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24601 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24661 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24721 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24781 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24841 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24901 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    24961 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25021 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25081 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25141 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25201 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25261 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25321 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25381 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcactgttag
    25441 gtgacaagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25501 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25561 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25621 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25681 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25741 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25801 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25861 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25921 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    25981 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26041 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26101 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26161 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26221 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26281 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26341 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26401 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26461 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26521 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26581 tcgtccggtt gcggacaaat ttaatcaaac tgaaacagct gccggttaat tactaagggg
    26641 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26701 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26761 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26821 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26881 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    26941 cttctgtaac gtagttgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27001 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27061 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27121 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27181 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27241 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27301 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27361 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27421 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27481 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27541 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27601 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27661 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27721 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27781 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27841 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27901 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    27961 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28021 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28081 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28141 tttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28201 cgagcagtct gcttatattc a
//
LOCUS       DAZPEU010000032        28183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPEU010000032 DAZPEU010000000
VERSION     DAZPEU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.032"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHU2L_001308"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHU2L_001308"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR1356243.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHU2L_001309"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHU2L_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR1356244.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHU2L_001310"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHU2L_001310"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR1356245.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHU2L_001311"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHU2L_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1356246.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHU2L_001312"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHU2L_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1356247.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHU2L_001313"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHU2L_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1356248.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHU2L_001314"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHU2L_001314"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1356249.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9168
                     /gene="atpI"
                     /locus_tag="ACHU2L_001315"
                     /pseudo
     CDS             8747..9168
                     /gene="atpI"
                     /locus_tag="ACHU2L_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
     gene            9177..9992
                     /gene="atpB"
                     /locus_tag="ACHU2L_001316"
     CDS             9177..9992
                     /gene="atpB"
                     /locus_tag="ACHU2L_001316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1356250.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            10039..10278
                     /gene="atpE"
                     /locus_tag="ACHU2L_001317"
     CDS             10039..10278
                     /gene="atpE"
                     /locus_tag="ACHU2L_001317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1356251.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10340..10810
                     /gene="atpF"
                     /locus_tag="ACHU2L_001318"
     CDS             10340..10810
                     /gene="atpF"
                     /locus_tag="ACHU2L_001318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1356252.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10825..11358
                     /gene="atpH"
                     /locus_tag="ACHU2L_001319"
     CDS             10825..11358
                     /gene="atpH"
                     /locus_tag="ACHU2L_001319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1356253.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11371..12912
                     /gene="atpA"
                     /locus_tag="ACHU2L_001320"
     CDS             11371..12912
                     /gene="atpA"
                     /locus_tag="ACHU2L_001320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1356254.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12963..13826
                     /gene="atpG"
                     /locus_tag="ACHU2L_001321"
     CDS             12963..13826
                     /gene="atpG"
                     /locus_tag="ACHU2L_001321"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1356255.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13853..15235
                     /gene="atpD"
                     /locus_tag="ACHU2L_001322"
     CDS             13853..15235
                     /gene="atpD"
                     /locus_tag="ACHU2L_001322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1356256.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15256..15675
                     /locus_tag="ACHU2L_001323"
     CDS             15256..15675
                     /locus_tag="ACHU2L_001323"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1356257.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            16026..17396
                     /gene="glmU"
                     /locus_tag="ACHU2L_001324"
     CDS             16026..17396
                     /gene="glmU"
                     /locus_tag="ACHU2L_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1356258.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17558..19387
                     /gene="glmS"
                     /locus_tag="ACHU2L_001325"
     CDS             17558..19387
                     /gene="glmS"
                     /locus_tag="ACHU2L_001325"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1356259.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19549..20370
                     /locus_tag="ACHU2L_001326"
     CDS             19549..20370
                     /locus_tag="ACHU2L_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR1356260.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20357..22465
                     /locus_tag="ACHU2L_001327"
     CDS             20357..22465
                     /locus_tag="ACHU2L_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1356261.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22462..24129
                     /locus_tag="ACHU2L_001328"
     CDS             22462..24129
                     /locus_tag="ACHU2L_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1356262.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24132..25658
                     /locus_tag="ACHU2L_001329"
     CDS             24132..25658
                     /locus_tag="ACHU2L_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR1356263.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25659..27275
                     /locus_tag="ACHU2L_001330"
     CDS             25659..27275
                     /locus_tag="ACHU2L_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR1356264.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27331..27483)
                     /locus_tag="ACHU2L_001331"
                     /pseudo
     CDS             complement(27331..27483)
                     /locus_tag="ACHU2L_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(27506..27883)
                     /locus_tag="ACHU2L_001332"
     CDS             complement(27506..27883)
                     /locus_tag="ACHU2L_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356265.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         7297 a         6549 c         7273 g         7064 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcagtctt tctgcctaac
     8941 gttttgttta tgatatttgc ctggcgtcac caggcgcata caccagcgaa aggccgggtg
     9001 gcttggacat tcgcatttgg cgaagctttc aaagttctgg cgatgttggt gttactggtg
     9061 gtggcgttgg cggttttaaa ggcggtattc ttgccgctga tcgttacgtg ggttttggtg
     9121 ctggtggttc agatactggc accggctgta attaacaaca aagggtaaaa ggcatcatgg
     9181 cttcagaaaa tatgacgccg caggattaca taggacacca cctgaataac cttcagctgg
     9241 acctgcgtac attctcgctg gtggatccac aaaacccccc agccaccttc tggacaatca
     9301 atattgactc catgttcttc tcggtggtgc tgggtctgtt gttcctggtt ttattccgta
     9361 gcgtagccaa aaaggcgacc agcggtgtgc caggtaagtt tcagaccgcg attgagctgg
     9421 tgatcggctt tgttaatggt agcgtgaaag acatgtacca tggcaaaagc aagctgattg
     9481 ctccgctggc cctgacgatc ttcgtctggg tattcctgat gaatctgatg gatttactgc
     9541 ctatcgactt gctgccgtac attgctgaac atgtactggg tctgcctgca ctgcgtgtgg
     9601 ttccgtctgc ggacgtgaac gtaacgctgt ctatggcact gggcgtattt atcctgattc
     9661 tgttctacaa catcaaaatg aaaggcatcg gcggcttcac gaaagagttg acgctgcagc
     9721 cgttcaatca ctgggcgttc attcctgtca acttaatcct tgaaggggta agcctgctgt
     9781 ccaaaccagt ttcactcggt ttgcgactgt tcggtaacat gtatgccggt gagctgattt
     9841 tcattctgat tgctggtctg ttgccgtggt ggtcacagtg gatcctgaat gtgccgtggg
     9901 ccattttcca catcctgatc attacgctgc aagccttcat cttcatggtt ctgacgatcg
     9961 tctatctgtc gatggcgtct gaagaacatt aatttaccaa cactactacg ttttaactga
    10021 aacaaactgg agactgtcat ggaaaacctg aatatggatc tgctgtacat ggctgccgct
    10081 gtgatgatgg gtctggcggc aatcggtgct gcgatcggta tcggcatcct cgggggtaaa
    10141 ttcctggaag gcgcagcgcg tcaacctgat ctgattcctc tgctgcgtac tcagttcttt
    10201 atcgttatgg gtctggtgga tgctatcccg atgatcgctg taggtctggg tctgtacgtg
    10261 atgttcgctg tcgcgtagta agcgttgctt ttatttaaag agcaatatca gaacgttaac
    10321 taaatagagg cattgtgctg tgaatcttaa cgcaacaatc ctcggccagg ccatcgcgtt
    10381 tgtcctgttc gttctgttct gcatgaagta cgtatggccg ccattaatgg cagccatcga
    10441 aaaacgtcaa aaagaaattg ctgacggcct tgcttccgca gaacgagcac ataaggacct
    10501 tgaccttgca aaggccagcg cgaccgacca gctgaaaaaa gcgaaagcgg aagcccaggt
    10561 aatcatcgag caggcgaaca aacgccgctc gcagattctg gacgaagcga aagctgaggc
    10621 agaacaggaa cgtactaaaa tcgtggccca ggcgcaggca gaaattgaag ccgagcgtaa
    10681 acgtgcccgt gaagagctgc gtaagcaagt tgctatcctg gctgttgctg gcgccgagaa
    10741 gatcatcgaa cgttccgtgg atgaagctgc taacagcgac atcgtggata aacttgtcgc
    10801 tgaactgtaa ggagggaggg gctgatgtct gaatttatta cggtagctcg cccctacgcc
    10861 aaagcagctt ttgactttgc cgtcgaacac caaagtgtag aacgctggca ggacatgctg
    10921 acgtttgccg ccgaggtaac caaaaacgaa caaatggcag agcttctctc tggcgcgctt
    10981 gcgccagaaa cgctcgccga gtcgtttatc gcagtttgtg gtgagcaact ggacgaaaac
    11041 ggtcagaacc tgattcgggt aatggctgaa aatggtcgtc ttaacgcgct cccggatgtt
    11101 ctggagcagt ttattcacct tcgtgccgtg agtgaggcta ccgctgaggt agacgtcatt
    11161 tccgctgccg cactgagtga acaacagctc gcgaaaattt ctgctgcgat ggaaaaacgt
    11221 ctgtcacgca aagttaagct gaattgcaaa atcgataagt ctgtaatggc tggcgttatc
    11281 atccgagcgg gtgatatggt cattgatggc agcgtacgcg gtcgtcttga gcgccttgca
    11341 gacgtcttgc agtcttaagg ggactggagc atgcaactga attccaccga aatcagcgaa
    11401 ctgatcaagc agcgcattgc tcagttcaat gttgtgagtg aagctcacaa cgaaggtact
    11461 attgtttctg taagtgacgg tgttatccgc attcacggcc tggccgattg tatgcagggt
    11521 gaaatgatct ccctgccggg taaccgttac gctatcgcac tgaacctcga gcgcgactct
    11581 gtaggtgcgg ttgttatggg tccgtacgct gaccttgccg aaggcatgaa agttaagtgt
    11641 actggccgta tcctggaagt tccggttggc cgtggcctgc tgggccgtgt ggttaacact
    11701 ctgggtgcac caatcgacgg taaaggtccg ctggatcacg acggcttctc tgctgtagaa
    11761 gcaatcgctc cgggcgttat cgaacgtcag tccgtagatc agccggtaca gaccggttat
    11821 aaagccgttg actccatgat cccaatcggt cgtggtcagc gtgaattgat catcggtgac
    11881 cgtcagacag gtaaaaccgc actggctatc gatgccatca tcaaccagcg cgattccggt
    11941 atcaaatgta tctatgtcgc tatcggccag aaagcgtcca ccatttctaa cgtggtacgt
    12001 aaactggaag agcacggcgc actggctaac accatcgttg tggtagcaac cgcgtctgaa
    12061 tccgctgcac tgcaatacct ggcaccgtat gccggttgcg caatgggcga atacttccgt
    12121 gaccgcggtg aagatgcgct gatcatttac gatgacctgt ctaaacaggc tgttgcttac
    12181 cgtcagatct ccctgctgct ccgtcgtccg ccaggacgtg aagcattccc gggcgacgtt
    12241 ttctacctcc actctcgtct gctggagcgt gctgcacgtg ttaacgccga atacgttgaa
    12301 gccttcacca aaggtgaagt gaaagggaaa accggttctc tgaccgcact gccgattatc
    12361 gaaactcagg cgggtgacgt ttctgcgttc gttccgacca acgtaatctc cattaccgat
    12421 ggtcagatct tcctggaaac caacctgttc aacgccggta ttcgtcctgc ggttaacccg
    12481 ggtatttccg tatcccgtgt tggtggtgca gcacagacca agatcatgaa aaaactgtcc
    12541 ggtggtatcc gtaccgctct ggcacagtat cgtgaactgg cagcgttctc tcagtttgca
    12601 tccgaccttg acgatgcaac acgtaagcag cttgaccacg gtcagaaagt gaccgaactg
    12661 ctgaaacaga aacagtatgc gccgatgtcc gttgcgcagc agtctctggt tctgttcgca
    12721 gcagaacgtg gttacctggc ggatgttgaa ctgtcgaaaa ttggcagctt cgaagccgct
    12781 ctgctggctt acgtcgaccg tgatcacgct ccgttgatgc aagagatcaa ccagaccggt
    12841 ggctacaacg acgaaatcga aggcaagctg aaaggcatcc tcgattcctt caaagcaacc
    12901 caatcctggt aacgtctgga ggcttgcctt agggcaggcc gcaaggcatt gaggagaagc
    12961 tcatggccgg cgcaaaagag atacgtagta agatcgcaag cgtccagaac acgcaaaaga
    13021 tcactaaagc gatggagatg gtcgccgctt ccaaaatgcg taaatcgcag gatcgcatgg
    13081 cggccagccg tccttatgca gaaaccatgc gcaaagtgat tggtcacctt gcacacggta
    13141 atctggaata taagcaccct tacctggaag accgcgacgt taaacgcgtg ggctacctgg
    13201 tggtgtcgac cgaccgtggt ttgtgcggtg gtttgaacat taacctgttc aaaaaactgc
    13261 tggcggaaat gaagacctgg accgacaaag gcgttcaatg cgacctcgca atgatcggct
    13321 cgaaaggcgt gtcgttcttc aactccgtgg gcggcaatgt tgttgcccag gtcaccggca
    13381 tgggggataa cccttccctg tccgaactga tcggtccggt aaaagtgatg ttgcaggcct
    13441 acgacgaagg ccgtctggac aagctttaca ttgtcagcaa caaatttatt aacaccatgt
    13501 ctcaggttcc gaccatcagc cagctgctgc cgttaccggc atcagatgat gatgatctga
    13561 aacataaatc ctgggattac ctgtacgaac ccgatccgaa ggcgttgctg gataccctgc
    13621 tgcgtcgtta tgtcgaatct caggtttatc agggcgtggt tgaaaacctg gccagcgagc
    13681 aggccgcccg tatggtggcg atgaaagccg cgaccgacaa tggcggcagc ctgattaaag
    13741 agctgcagtt ggtatacaac aaagctcgtc aggccagcat tactcaggaa ctcaccgaga
    13801 tcgtctcggg ggccgccgcg gtttaaacag gttatttcgt agaggattta agatggctac
    13861 tggaaagatt gtccaggtaa tcggcgccgt agttgacgtc gaattccctc aggatgccgt
    13921 accgcgcgtg tacgatgctc ttgaggtgca aaatggtaat gagcgtctgg tgctggaagt
    13981 tcagcagcag ctcggcggcg gtatcgtgcg taccatcgca atgggttcct ccgacggtct
    14041 gcgtcgcggt ctggatgtaa aagacctcga acacccgatc gaagtcccgg taggtaaagc
    14101 gactctgggc cgtatcatga acgtactggg tgaaccggtc gacatgaaag gcgagatcgg
    14161 tgaagaagag cgttgggcga ttcaccgcgc agcaccttcc tacgaagagc tgtcaaactc
    14221 tcaggaactg ctggaaaccg gtatcaaagt tatcgacctg atgtgtccgt tcgctaaggg
    14281 cggtaaagtt ggtctgttcg gtggtgcggg tgtaggtaaa accgtaaaca tgatggagct
    14341 cattcgtaac atcgcgatcg agcactccgg ttactctgtg tttgcgggcg taggtgaacg
    14401 tactcgtgag ggtaacgact tctaccacga aatgaccgac tccaacgtta tcgacaaagt
    14461 atccctggtg tatggccaga tgaacgagcc gccgggaaac cgtctgcgcg ttgctctgac
    14521 cggtctgacc atggctgaga aattccgtga cgaaggtcgt gacgttctgc tgttcgttga
    14581 caacatctat cgttacaccc tggccggtac ggaagtatcc gcactgctgg gccgtatgcc
    14641 ttcagcggta ggttatcagc cgaccctggc ggaagagatg ggcgttctgc aggaacgtat
    14701 cacctccacc aaaactggtt ctatcacctc cgtacaggcg gtatacgtac ctgcggatga
    14761 cttgactgac ccgtctccgg caaccacctt tgcgcacctt gacgcaaccg tggtactgag
    14821 ccgtcagatc gcgtctctgg gtatctaccc ggccgttgac ccgctggact ccaccagccg
    14881 tcagctggac ccgctggtgg ttggtcagga acactacgac accgcgcgtg gcgttcagtc
    14941 catcctgcaa cgttatcagg aactgaaaga catcatcgcc atcctgggta tggatgaact
    15001 gtctgaagaa gacaaactgg tggtagcgcg tgctcgtaag atccagcgct tcctgtccca
    15061 gccgttcttc gtggcagaag tattcaccgg ttctccgggt aaatacgtct ccctgaaaga
    15121 caccatccgt ggctttaaag gcatcatgga aggcgaatac gatcacctgc cggagcaggc
    15181 gttctacatg gtcggttcca tcgaagaagc tgtggaaaaa gccaaaaaac tttaacgcct
    15241 taatcggagg gtgatatggc aatgacttac cacctggacg tcgtcagcgc agagcaacaa
    15301 atgttctctg gtctggtcga gaaaatccag gtaacgggta gcgaaggtga actggggatc
    15361 taccctggcc acgcaccgct gctcaccgcc attaagcctg gtatgattcg catcgtgaaa
    15421 cagcacggtc acgaagagtt tatctatctg tctggcggca ttcttgaagt gcagcctggc
    15481 aacgtgaccg ttctggccga caccgcaatt cgcggccagg atctcgacga agcgcgagcc
    15541 atggaagcga aacgtaaggc tgaagagcac attagcagct ctcacggcga cgtagattac
    15601 gctcaggcgt ctgcggaact ggccaaagcg atcgcgcagc tgcgcgttat cgagttgacc
    15661 aaaaaagcga tgtaacaccg gcttgaaaag cacaaaagcc agtctggaaa caggctggct
    15721 ttttttacgc gttgacccgt cctgaagagc gttcacatag atcctgctga tataaaacac
    15781 ccctgttttc ccgtttattc attgatcgaa ataagagcaa aaacatccac ctgacgctta
    15841 aatgaaggta ctgccttaat tttctgcaga taaaaggcgt gacgatggtc gaaaatggca
    15901 ctttcgtcag cggggataat ccgttattga acaatttatc ctctgtccat ttcacgatga
    15961 aaaaaatgta gttttttcaa ggtgaagcgg tttaaattcg ttctcaaatt acagtcagga
    16021 cgcgtatgtt gaataatgct atgagcgtag tgatccttgc cgcaggcaaa ggcacgcgca
    16081 tgtattccga tcttccgaaa gtgctgcata cccttgccgg gaaagcgatg gttcagcatg
    16141 tcattgatgc tgcgaatgaa ttaggcgcag cgcacgttca cctggtgtac ggtcacggcg
    16201 gcgatctgct taaacaggcg ctgaaagacg acaaccttaa ctgggtgctt caggcagagc
    16261 agctgggtac gggtcatgca atgcagcagg ccgcaccttt ctttgccgat gatgaagaca
    16321 ttttaatgct ctacggcgac gtgccgctga tctctgtcga aacactccag cgcctgcgtg
    16381 atgctaaacc ccagggtggc attggtctgc tgacggtgaa actggatgat ccgaccggtt
    16441 atggacgtat cacccgtgaa aatggcaaag ttaccggcat tgttgagcac aaagatgcca
    16501 ccgacgagca gcgtcagatt caggagatca acaccggcat tctgattgcc aacggcgcag
    16561 atatgaaacg ctggctggcg aagctgacca acaataatgc tcagggcgaa tactacatca
    16621 ccgacattat tgcgctggcg tatcaggaag ggcgtgaaat cgtcgccgtt catccgcaac
    16681 gtttaagcga agtagaaggt gtgaataacc gcctgcaact ctcccgtctg gagcgcgttt
    16741 accagtccga acaggctgaa aaactgctgt tagcaggcgt tatgctgcgc gatccggcgc
    16801 gttttgatct gcgcggtacg cttactcacg ggcgcgatgt tgaaattgat actaacgtta
    16861 tcatcgaggg caacgtgact ctcggtcatc gcgtgaaaat cggcaccggt tgcgtgatta
    16921 aaaacagcgt gattggcgat gattgcgaaa tcagtccgta taccgttgtg gaagacgcga
    16981 atctggcagc ggcctgtact attggcccgt ttgcccgtct gcgtcctggt gctgagttgc
    17041 tggaaggtgc acacgttggt aacttcgttg agatgaaaaa agcgcgtctg ggtaaaggca
    17101 cgaaagccgg gcatctgact tacctgggcg atgcggaaat tggcgataac gttaacatcg
    17161 gcgcgggaac cattacctgc aactacgatg gtgcgaataa atttaagacc attatcggcg
    17221 acgatgtgtt tgtcggttcc gacactcagc tggtggcccc ggtaacagta ggcaaaggcg
    17281 cgaccattgc tgcgggtaca actgtgacgc gtaatgtcgg cgaaaatgca ttagctatca
    17341 gccgtgtgcc gcagactcag aaagaaggct ggcgtcgtcc ggtaaagaaa aagtaattct
    17401 ggccgggtaa tccggtcatc tgggatgagg agataacata atctccctcc cacaagcagt
    17461 aactataaaa ataaccccac tctctacaag gctcggggcg cccgaaaaaa cgggcataca
    17521 ggttgaccga caacgatata aatcggaatc aaaaactatg tgtggaattg ttggcgcgat
    17581 cgcgcaacgt gatgtagcag aaatccttct tgaaggttta cgtcgtctgg aataccgcgg
    17641 atatgactct gccggtctgg ccgttgttga tgcggaaggt catatgactc gcctgcgtcg
    17701 cctcggtaaa gtccagatgc tggctcaggc agcggaagaa catcctctgc atggcggcac
    17761 cggtattgct cacactcgct gggcgaccca cggtgaacct tcagaagcta acgcgcatcc
    17821 gcatgtttct gaacacattg tggtggtgca taatggcatc atcgaaaacc atgaaccgct
    17881 gcgtgaagag ttaaaagcgc gtggctatac cttcgtttct gaaaccgaca ccgaagtgat
    17941 tgcccatctg gtgaactggg agctgaaaca aggcgggact ctgcgtgagg ctgttctgcg
    18001 tgctatcccg cagctgcgcg gtgcgtacgg tacagtgatc atggactccc gtcacccgga
    18061 taccctgcta gcggcacgtt ctggtagtcc gctggtgatt ggcctgggta tgggcgaaaa
    18121 ctttatcgct tctgaccagc tggcgctgtt gccggtgacc cgtcgcttta tcttccttga
    18181 agagggcgat attgcggaaa tcactcgccg ttcggtaaac atcttcgata aaactggcgc
    18241 ggaagtaaaa cgtcaggata tcgaatccaa tctgcaatat gacgcgggcg ataaaggtat
    18301 ttaccgtcac tacatgcaga aagagatcta cgaacagccg aacgcgataa aaaacaccct
    18361 taccgggcgc atcagccacg gtcaggttga tttaagcgag ctgggaccga acgccgacga
    18421 actgctgtcg aaggttgagc atattcagat cctcgcctgt ggtacttctt ataactccgg
    18481 tatggtttcc cgctactggt ttgaatcgct ggcaggtatt ccgtgcgacg tcgaaatcgc
    18541 ctctgaattc cgctatcgta aatctgccgt gcgtcgtaac agcctgatga tcaccttgtc
    18601 acagtctggc gaaaccgcgg ataccctggc gggcctgcgt ctgtcgaaag agctgggtta
    18661 ccttggttcg ctggcaatct gtaacgttcc gggttcttct ctggtgcgcg aatccgatct
    18721 ggcgctgatg accaacgcgg gtacggaaat cggcgtggcg tccactaaag cattcaccac
    18781 tcagttaact gtgctgttga tgctggtggc gaagctgtct cgcctgaaag gtctggatgc
    18841 ctccattgaa catgacattg tgcatggtct gcaggcgctg ccgagccgta ttgagcagat
    18901 gctgtctcag gataaacgca ttgaagctct ggcagaagat ttctctgaca aacatcacgc
    18961 gctgttcctg ggccgtggcg atcagtaccc aatcgcgctg gaaggcgcat tgaagctgaa
    19021 agagatctct tacattcacg ctgaagccta cgctgcaggc gagctgaaac acggcccgct
    19081 ggcgctgatt gacgcggata tgccggttat cgtcgttgca ccgaacaacg aactgttgga
    19141 aaaactgaaa tccaacattg aagaagttcg cgcgcgtggc ggtcaattgt atgtcttcgc
    19201 cgatcaggat gcgggttttg taagtagcga taacatgcac atcatcgaga tgccgcatgt
    19261 ggaagaggtg attgcaccga tcttctacac cgttccgctg cagctgctgg cttaccatgt
    19321 cgcgctgatc aaaggcaccg acgttgacca gccgcgtaac ctggcaaaat cggttacggt
    19381 tgagtaatca ctatcataga taaaaaaggg ctgctgtggg cggacaataa agtcttaaac
    19441 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
    19501 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
    19561 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
    19621 catggtaaag actatattcc atggctaaca gtacaagaag ttccttcttc aggtcgttcc
    19681 caccgtattt attctcataa gacgggacga gtccatcatt tgctatctga cttagagctt
    19741 gctgtttttc tcagtcttga gtgggagagc agcgtgctag atatacgcga gcagttcccc
    19801 ttattaccta gtgataccag gcagattgca atagatagtg gtattaagca tcctgttatt
    19861 cgtggtgtag atcaggttat gtctactgat tttttagtgg actgcaaaga tggtcctttt
    19921 gagcagtttg ctattcaagt caaacctgca gcagccttac aagacgagcg taccttagaa
    19981 aaactagaac tagagcgtcg ctattggcag caaaagcaaa ttccttggtt catttttact
    20041 gataaagaaa taaatcccgt agtaaaagaa aatattgaat ggctttattc agtgaaaaca
    20101 gaagaagttt ctgcggagct tttagcacaa ctatccccat tggcccatat cctgcaagaa
    20161 aaaggagatg aaaacattat caatgtctgt aagcaggttg atattgctta tgatttggag
    20221 ttaggcaaaa cattgagtga gatacgagcc ttaaccgcaa atggttttat taagttcaat
    20281 atttataagt ctttcagggc aaataagtgt gcagatctct gtattagcca agtagtgaat
    20341 atggaggagt tgcgctatgt ggcaaattaa tgaggttgtg ctatttgata atgatccgta
    20401 tcgcattttg gctatagagg atggccaagt tgtctggatg caaataagcg ctgataaagg
    20461 agttccacaa gctagggctg agttgttgct aatgcagtat ttagatgaag gccgcttagt
    20521 tagaactgat gacccttatg tacatcttga tttagaagag ccgtctgtag attctgtcag
    20581 cttccagaag cgcgaggagg attatcgaaa aattcttcct attattaata gtaaggatcg
    20641 tttcgaccct aaagtcagaa gcgaactcgt tgagcatgtg gtccaagaac ataaggttac
    20701 taaggctaca gtttataagt tgttacgccg ttactggcag cgtggtcaaa cgcctaatgc
    20761 attaattcct gactacaaaa acagcggtgc accaggggaa agacgttcag cgacaggaac
    20821 agcaaagatt ggccgagcca gagaatatgg taagggtgaa ggaaccaagg taacgcccga
    20881 gattgaacgc ctttttaggt tgaccataga aaagcacctg ttaaatcaaa aaggtacaaa
    20941 gaccaccgtt gcctatagac gatttgtgga cttgtttgct cagtattttc ctcgcattcc
    21001 ccaagaggat tacccaacac tacgtcagtt tcgttatttt tatgatcgag aataccctaa
    21061 agctcagcgc ttaaagtcta gagttaaagc aggggtatat aaaaaagacg tacgaccctt
    21121 aagtagtaca gccacttctc aggcgttagg ccctgggagt cgttatgaga ttgatgccac
    21181 gattgctgat atttatttag tggatcatca tgatcgccaa aaaatcatag gaagaccaac
    21241 gctttacatt gtgattgatg tgtttagtcg gatgatcacg ggcttttata tcggctttga
    21301 aaatccgtct tatgtggtgg cgatgcaggc ttttgtaaat gcttgctctg acaaaacggc
    21361 catttgtgcc cagcatgata ttgagattag tagctcagac tggccgtgtg taggtttgcc
    21421 agatgtgttg ctagcggacc gtggcgaatt aatgagtcat caggtcgaag ccttagtttc
    21481 tagttttaat gtgcgagtgg aaagtgctcc acctagacgt ggcgatgcta aaggcatagt
    21541 ggaaagcact tttagaacac tacaagccga gtttaagtcc tttgcacctg gcattgtaga
    21601 gggcagtcgg atcaaaagcc atggtgaaac agactatagg ttagatgcat ctctgtcggt
    21661 atttgagttc acacaaatta ttttgcgtac gatcttattc agaaataacc atctggtgat
    21721 ggataaatac gatcgagatg ctgattttcc tacagattta ccgtctattc ctgtccagct
    21781 atggcaatgg ggtatgcagc atcgtacagg tagtttaagg gctgtggagc aagagcagtt
    21841 gcgagtagcg ttactgcctc gccgaaaggt ctctatttct tcatttggcg ttaatttgtg
    21901 gggtttgtat tactcggggt cagagattct gcgtgagggt tggttgcagc ggagcactga
    21961 tatagctaga cctcaacatt tagaagcggc ttatgaccca gtgctggttg atacgattta
    22021 tttgtttccg caagttggca gccgtgtatt ttggcgctgt aatctgacgg aacgtagtcg
    22081 gcagtttaaa ggtctctcat tttgggaggt ttgggatata caagcacaag aaaaacacaa
    22141 taaagccaat gcgaagcagg atgagttaac taaacgcagg gagcttgagg cgtttattca
    22201 gcaaaccatt cagaaagcga ataagttaac gcccagtact actgagccca aatcaacacg
    22261 cattaagcag attaaaacta ataaaaaaga agccgtgacc tcggagcgta aaaaacgtgc
    22321 ggagcatttg aagccaagct cttcaggtga tgaggctaaa gttattcctt tcaacgcagt
    22381 ggaagcggat gatcaagaag attacagcct acccacatac gtgcctgaat tatttcagga
    22441 tccaccagaa aaggatgagt catgagtgct acccggattc aagcagttta tcgtgatacg
    22501 ggggtagagg cttatcgtga taatcctttt atcgaggcct taccaccatt acaagagtca
    22561 gtgaatagtg ctgcatcact gaaatcctct ttacagctta cttcctctga cttgcaaaag
    22621 tcccgtgtta tcagagctca taccatttgt cgtattccag atgactattt tcagccatta
    22681 ggtacgcatt tgctactaag tgagcgtatt tcggtcatga ttcgaggtgg ctacgtaggc
    22741 agaaatccta aaacaggaga tttacaaaag catttacaaa atggttatga gcgtgttcaa
    22801 acgggagagt tggagacatt tcgctttgag gaggcacgat ctacggcaca aagcttattg
    22861 ttaattggtt gttctggtag tgggaagacg acctctcttc atcgtattct agccacgtat
    22921 cctcaggtga tttaccatcg tgaactcaat gtagagcagg tggtgtattt gaaaatagac
    22981 tgctcgcata atggttcgct aaaagaaatc tgcttgaatt ttttcagagc gttggatcga
    23041 gccttgggct cgaactatga gcgtcgttat ggcttaaaac gtcatggtat agaaaccatg
    23101 ttggctttga tgtcgcaaat agccaatgca catgctttag ggttgttggt tattgatgaa
    23161 attcagcatt taagccgctc tcgttcgggt ggatctcaag agatgctgaa cttttttgtg
    23221 acgatggtga atattattgg cgtaccagtg atgttgattg gtacccctaa agcacgagag
    23281 atttttgagg ctgatttgcg gtctgcacgt agaggggcag ggtttggagc tatattctgg
    23341 gatcctatac aacaaacgca acgtggaaag cccaatcaag agtggatcgc ttttacggat
    23401 aatctttggc aattacagct tttacaacgc aaagatgcgc tgttatcgga tgaggtccgt
    23461 gatgtgtggt atgagctaag ccaaggagtg atggacattg tagtaaaact ttttgtactc
    23521 gctcagctcc gtgcgctagc tttaggcaat gagcgtatta ccgctggttt attgcggcaa
    23581 gtgtatcaag atgagttaaa gcctgtgcac cccatgctag aggcattacg ctcgggtatc
    23641 ccagaacgca ttgctcgtta ttctgatcta gtcgttcccg agattgataa acggttaatc
    23701 caacttcagc tagatatcgc agcgatacaa gaacaaacac cagaagaaaa agcccttcaa
    23761 gagttagata ccgaagatca gcgtcattta tatctgatgc tgaaagagga ttacgattca
    23821 agcctgttaa ttcccactat taaaaaagcg tttagccaga atccaacgat gacaagacaa
    23881 aagttactgc ctcttgtttt gcagtggttg atggaaggcg aaacggtagt gtcagaacta
    23941 gaaaagccct ccaagagtaa aaaggtttcg gctataaagg tagtcaagcc cagcgactgg
    24001 gatagcttgc ctgatacgga tttacgttat atctattcac aacgccaacc tgaaaaaacc
    24061 atgcatgaac ggttaaaagg gaaaggggta atagtggata tggcgagctt atttaaacaa
    24121 gcaggttagc catgagaaac tttcctgttc cgtactcgaa tgagctgatt tatagcacta
    24181 ttgcacgggc aggcgtttat caagggattg ttagtcctaa gcagctgttg gatgaggtgt
    24241 atggcaaccg caaggtggtc gctaccttag gtctgccctc gcatttaggt gtgatagcaa
    24301 gacatctaca tcaaacagga cgttacgctg ttcagcagct tatttatgag cataccttat
    24361 tccctttata tgctccgttt gtaggcaagg agcgccgaga cgaagctatt cggttaatgg
    24421 agtaccaagc gcaaggtgcg gtgcatttaa tgctaggagt cgctgcttct agagttaaga
    24481 gcgataaccg ctttagatac tgccctgatt gcgttgctct tcagctaaat aggtatgggg
    24541 aagccttttg gcaacgagat tggtatttgc ccgctttgcc atattgtcca aaacacggtg
    24601 ctttagtctt ctttgataga gctgtagatg atcaccgaca tcaattttgg gctttgggtc
    24661 atactgagct gctttcagac taccccaaag actccctatc tcaattaaca gcactagctg
    24721 cttatatagc ccctctgtta gatgctccac gagcgcaaga gctttcccca agccttgagc
    24781 agtggacgct gttttatcag cgcttagcgc aggatctagg gctaaccaaa agcaagcaca
    24841 ttcgtcatga cttggtggcg gagagagtga ggcagacttt tagtgatgag gcactagaga
    24901 aactggattt aaagttggca gagaacaagg acacgtgttg gctgaaaagt atattccgta
    24961 agcatagaaa agcctttagt tatttacagc atagtattgt gtggcaagcc ttattgccaa
    25021 aactaacggt tatagaagcg ctacagcagg caagtgctct tactgagcac tctataacga
    25081 caagacctgt tagccagtct gtgcaaccta actctgaaga tttatctgtt aagcataaag
    25141 actggcagca actagtgcat aaataccaag gaattaaggc ggcaagacag tctttagagg
    25201 gtggggtgct atacgcttgg ctttaccgac atgacaggga ttggctagtt cactggaatc
    25261 aacagcatca acaagagcgt ctggcacccg cccctagagt tgattggaac caaagagatc
    25321 gaattgctgt acgacaacta ttaagaatca taaagcgtct agatagtagc cttgatcacc
    25381 caagagcgac atcgagctgg ctgttaaagc aaactcctaa cggaacctct cttgcaaaaa
    25441 atctacagaa actgcctttg gtagcgcttt gcttaaagcg ttactcagag agtgtggaag
    25501 attatcaaat tagacggatt agccaagctt ttattaagct taaacaggaa gatgttgagc
    25561 ttaggcgctg gcgattatta agaagtgcaa cgttatctaa agagcggata actgaggaag
    25621 cacaaagatt cttggaaatg gtttatgggg aagagtgagt ggttaggcta gctacattta
    25681 atgacaatgt gcaggttgta catattggtc atttattccg taactcgggt cataaggagt
    25741 ggcgtatttt tgtttggttt aatccaatgc aagaacggaa atggactcga tttactcatt
    25801 tgcctttatt aagtcgagct aaggtggtta acagtacaac aaagcaaata aataaggcgg
    25861 atcgtgtgat tgagtttgaa gcatcggatc ttcaacgagc caaaataatc gattttccta
    25921 atctctcgtc ctttgcttcc gtacgcaaca aggatggagc gcagagttca tttatttacg
    25981 aagctgaaac accatatagc aagactcgtt atcacatccc acagttagag ctagctcggt
    26041 cattattttt aattaactcc tatttctgtc gaagctgttt gagcagtacc gctttacagc
    26101 aagagttcga cgttcagtat gaggttgagc gagatcattt agagataagg atcttaccca
    26161 gttcatcgtt tcctaaaggg gcgttagagc tgtcggccgt agtgcagctt ttggtttggt
    26221 tgttttcgga tcaagatgtt atggattcgt atgaaagtat ttttaggcac tatcaacaaa
    26281 atagagaaat taagaacggc gttgaaagct ggtgctttag ctttgaccct ccgcccatgc
    26341 agggttggaa attacatgta aaaggacgtt cttctaacga ggataaggat tatttagttg
    26401 aggaaatagt aggtttagaa atcaacgcta tgcttcctag cacaacagct attagccatg
    26461 cctcttttca ggaaaaggag gcaggtgatg gtagtacgca gcacatagcg gtttcaacag
    26521 agtcagttgt tgatgatgag catctacagt tggacgatga ggaaacagcc aatatagaca
    26581 cagacacacg agtcatagag gctgagccga catggataag ttttagtaga cctagtcgaa
    26641 ttgaaaaatc tcgcagggca agaaaaagta gccaaactat tttagaaaaa gaagaagcaa
    26701 caacaagtga aaatagtaat ttggttagta ctgatgagcc acacttaggt ggtgtcctag
    26761 cagcggcaga tgtgggtggg aagcaggatg caaccaatta caactctatt tttgctaatc
    26821 gatttgctgc ttttgatgag ctactttcaa ttctaaaaac taaatttgca tgtcgggtgc
    26881 tttttgaaga aaccttggtt ttgccaaaag ttgggcgtag ccgattacat ctgtgtaaag
    26941 atggctcacc aagagtgatt aaagccgttg gggtgcaacg taatggcagt gaatttgtat
    27001 tgctagaggt ggatgcatcg gatggggtga aaatgctttc taccaaagtg ttgagtggcg
    27061 ttgatagcga aacatggcgg aatgattttg aaaagatacg gcgtggagtg gtgaagagct
    27121 cattgaattg gccaaatagt ttgtttgatc aattatatgg acaagacggg catagagggg
    27181 tgaatcatcc aaaggggttg ggggagctgc aagtatcgag agaggatatg gaagggtggg
    27241 ctgagagagt ggttagagag caatttacgc attaaaggaa tgactgaaag agcctgtaaa
    27301 cccttttgtg taagtgcttt tgccggtcag ttaaaggtgg ccatttaaac ggtcaccaaa
    27361 ttcgatcata aaacggttca tggccggctt ccagttgcgg atcggcatcg tccatttctt
    27421 ggtcgccgcc tggatagcca ggtacaccac cttcatcgct gattcgtccg tagggaacac
    27481 cttgagttca tattaatgga attttctaca aatagcctcc gtggttttga ggggggatta
    27541 cagacgatcc atagtagtaa tccaatgagt tcttgagcgc ggcgacatgt ttggacgcct
    27601 tggcaaaaat tagagcctgc ttgaagtgca ggcgagcacg tgcttggacg attgatccat
    27661 tcgcggtcaa aaactcaatc ttggatgaca gcgtgtcagg aaatccaata tcgaagtccc
    27721 gccccgtgtc aaagtagggc atttcatgat caaaggacga agcttcccag gccacttccc
    27781 taatattcca gccgctagaa acaagatttc gtacgacttc atcaactttt ttgctttcca
    27841 acttgtcaca aagtgcatag gcaagagctt tggcggcaga cacgtgcgtt tcctcttaaa
    27901 cataggtcga acggataccc gaggcaaagc ggagcattag tcccaaagca tctccggtag
    27961 atccggaggc tccatactga aggcatggca tcttgggcat tgatatttga aacttctctc
    28021 tagatagcag ggatttcttg gccttgcctc atgctgatag cttgtaaata tcacacgatc
    28081 cttagcccca caaagtgagc atactccgta gctttcattt atagaaatcg tcgttagatt
    28141 gtcgcagcat gtgcacgttc cgactatttg ctcaggaagc atc
//
LOCUS       DAZPEU010000033        27071 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPEU010000033 DAZPEU010000000
VERSION     DAZPEU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.033"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..175)
                     /locus_tag="ACHU2L_001333"
     CDS             complement(<1..175)
                     /locus_tag="ACHU2L_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR1356266.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="ACHU2L_001334"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="ACHU2L_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1356267.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="ACHU2L_001335"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="ACHU2L_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR1356268.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="ACHU2L_001336"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="ACHU2L_001336"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR1356269.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="ACHU2L_001337"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="ACHU2L_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR1356270.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="ACHU2L_001338"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="ACHU2L_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR1356271.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="ACHU2L_001339"
     CDS             complement(5179..5421)
                     /locus_tag="ACHU2L_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR1356272.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="ACHU2L_001340"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="ACHU2L_001340"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR1356273.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="ACHU2L_001341"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="ACHU2L_001341"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR1356274.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAKSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="ACHU2L_001342"
     CDS             complement(8338..9312)
                     /locus_tag="ACHU2L_001342"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR1356275.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="ACHU2L_001343"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="ACHU2L_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR1356276.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="ACHU2L_001344"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="ACHU2L_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR1356277.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="ACHU2L_001345"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="ACHU2L_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR1356278.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="ACHU2L_001346"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="ACHU2L_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR1356279.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="ACHU2L_001347"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="ACHU2L_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR1356280.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="ACHU2L_001348"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="ACHU2L_001348"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR1356281.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="ACHU2L_001349"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="ACHU2L_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR1356282.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="ACHU2L_001350"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="ACHU2L_001350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR1356283.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="ACHU2L_001351"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="ACHU2L_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR1356284.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /gene="aaeX"
                     /locus_tag="ACHU2L_001352"
     CDS             23179..23382
                     /gene="aaeX"
                     /locus_tag="ACHU2L_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR1356285.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="ACHU2L_001353"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="ACHU2L_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR1356286.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="ACHU2L_001354"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="ACHU2L_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR1356287.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTHFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="ACHU2L_001355"
     CDS             26387..26659
                     /locus_tag="ACHU2L_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR1356288.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="ACHU2L_001356"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="ACHU2L_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR1356289.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6456 a         6790 c         7229 g         6596 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaactggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatctgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagagtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgggcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattta gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctcac cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac tggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcatttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg acaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat a
//
LOCUS       DAZPEU010000034        27033 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPEU010000034 DAZPEU010000000
VERSION     DAZPEU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.034"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHU2L_001357"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHU2L_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR1356290.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHU2L_001358"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHU2L_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR1356291.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHU2L_001359"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHU2L_001359"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR1356292.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHU2L_001360"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHU2L_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHU2L_001361"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHU2L_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR1356293.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHU2L_001362"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHU2L_001362"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR1356294.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHU2L_001363"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHU2L_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR1356295.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="ACHU2L_001364"
     CDS             7490..7639
                     /locus_tag="ACHU2L_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356296.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="ACHU2L_001365"
     CDS             7840..9423
                     /locus_tag="ACHU2L_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1356297.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="ACHU2L_001366"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="ACHU2L_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR1356298.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="ACHU2L_001367"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="ACHU2L_001367"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR1356299.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="ACHU2L_001368"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="ACHU2L_001368"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR1356300.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..13624
                     /locus_tag="ACHU2L_001369"
                     /pseudo
     CDS             13382..13624
                     /locus_tag="ACHU2L_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13623..14564
                     /gene="mdtA"
                     /locus_tag="ACHU2L_001370"
                     /pseudo
     CDS             13623..14564
                     /gene="mdtA"
                     /locus_tag="ACHU2L_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14564..17686
                     /gene="mdtB"
                     /locus_tag="ACHU2L_001371"
                     /pseudo
     CDS             14564..17686
                     /gene="mdtB"
                     /locus_tag="ACHU2L_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17687..20752
                     /gene="mdtC"
                     /locus_tag="ACHU2L_001372"
     CDS             17687..20752
                     /gene="mdtC"
                     /locus_tag="ACHU2L_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR1356301.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20753..22168
                     /locus_tag="ACHU2L_001373"
     CDS             20753..22168
                     /locus_tag="ACHU2L_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1356302.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22165..23568
                     /gene="baeS"
                     /locus_tag="ACHU2L_001374"
     CDS             22165..23568
                     /gene="baeS"
                     /locus_tag="ACHU2L_001374"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR1356303.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23565..24287
                     /gene="baeR"
                     /locus_tag="ACHU2L_001375"
     CDS             23565..24287
                     /gene="baeR"
                     /locus_tag="ACHU2L_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR1356304.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24478..24810
                     /locus_tag="ACHU2L_001376"
     CDS             24478..24810
                     /locus_tag="ACHU2L_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR1356305.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24957..26318
                     /gene="yegQ"
                     /locus_tag="ACHU2L_001377"
     CDS             24957..26318
                     /gene="yegQ"
                     /locus_tag="ACHU2L_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR1356306.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26660..26977)
                     /gene="yegR"
                     /locus_tag="ACHU2L_001378"
     CDS             complement(26660..26977)
                     /gene="yegR"
                     /locus_tag="ACHU2L_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR1356307.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6155 a         7236 c         7104 g         6538 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggagcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata cagttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 cattccagga aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc
    13681 agttcaaagt tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg
    13741 ctaacgcccg ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc
    13801 gtcaggagct ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg
    13861 atgaagcaag cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag
    13921 tcgatggtcg cgttggtctc aagcaagttg atgttggtaa ccaaatctcc agtggtgata
    13981 ccaccggaat tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg
    14041 aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg atggtagaag
    14101 cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc
    14161 aaatcgatgc cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg
    14221 cgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg
    14281 tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga
    14341 atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag
    14401 tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc
    14461 tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga
    14521 aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc
    14581 gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct
    14641 gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct
    14701 gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga
    14761 tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct
    14821 gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct
    14881 aacattaccg ctcaatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa
    14941 cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc
    15001 gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat
    15061 ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct
    15121 ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct
    15181 cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa
    15241 aggtagcctc gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc
    15301 cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg
    15361 cgatgtcgca actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa
    15421 agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc
    15481 cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt
    15541 gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga
    15601 attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc
    15661 ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat
    15721 ggtgtttctc gatttttcaa tcaataacct gacactgatg gctttaacta tcgccaccgg
    15781 attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg
    15841 cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc
    15901 gctgaccttc tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg
    15961 gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt
    16021 gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa
    16081 acagaacagc ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg
    16141 tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac
    16201 gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga
    16261 caacggcatt attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc
    16321 ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac
    16381 ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa
    16441 cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac
    16501 ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat
    16561 cgatactcag gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc
    16621 gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac agctttctga
    16681 tgtctccagc gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag
    16741 cgccagccgt ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg
    16801 tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggagca
    16861 caataccgaa aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga
    16921 cggtggcgtg gtgccgttaa gctcaattgc caaaattgag cagcgttttg cgccgctttc
    16981 catcaaccat ctggatcagt tcccggtaac aaccatctcc tttaacgtgc cggataacta
    17041 ttcgctgggc gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt
    17101 ggatatcacc acgcaattct agggcagcac cctcgccttc cagtcggcgc tgggcagcac
    17161 tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga
    17221 gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct
    17281 gctggcatta atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt
    17341 gctgatcggt attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga
    17401 gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg
    17461 tccgatcctg atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac
    17521 cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt
    17581 cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc
    17641 attgtggacc aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc
    17701 cctcttcatt taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg
    17761 catactgggt ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat
    17821 tatggtcagc gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac
    17881 gccgctggag cgctcacttg ggcgcattgc cggggttagt gaaatgacct ccagcagttc
    17941 gctcggcagc acgcgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc
    18001 gcgtgatgtg caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag
    18061 ccgcccgacc tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac
    18121 atccgatact tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac
    18181 gatttcgcaa atcgacggtg tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt
    18241 gcgcgtcggg ctgaccccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg
    18301 cactgccatc agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca
    18361 ccgctggcag atccagacca acgatgaact gaaaaccgcc gctgaatatc agccgctgat
    18421 tattcactac aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgactgactc
    18481 agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat
    18541 ccgcaaactg ccggaagcca atattatcca gacggtagac agcatccggg caaaattacc
    18601 agagttgcag gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc
    18661 caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt
    18721 gattctggtg gtctttttat tcctgcgctc gggtcgcgcc actattattc ccgccgttgc
    18781 ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa
    18841 taatctttcg ctgatggcgc tcaccattgc tactggtttc gtggtggatg acgccattgt
    18901 ggtgctggaa aacattgccc gtcatctgga agcggggatg aaaccactac aggccgcgct
    18961 gcaaggtact cgcgaagtcg gctttacggt gctgtcgatg agtctgtcac tggtggcggt
    19021 gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt
    19081 gacgctttct gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat
    19141 gtgcggctgg atgctaaaag ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg
    19201 ccgcatgttg gtagccctgc aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca
    19261 tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct cgatcccgaa
    19321 aaccttcttc ccggagcagg acaccggcgt actgatgggt gggatacagg cggatcagag
    19381 tatttcgttt caggcgatgc gcggcaaatt gcaggatttc atgaaaatta tccgtgacga
    19441 tccggcagtg gataatgtca ccggctttac cggcggttca cgagttaaca gtgggatgat
    19501 gtttatcacc ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg
    19561 tctgcgcgtg aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga
    19621 tattcgcgtt ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga
    19681 cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga
    19741 actggcggac gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga
    19801 ccgcgacacc atggcacggc tgggaatcga cgtacaggcc gccaacagtc tgttaaataa
    19861 cgccttcggt cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt
    19921 gatggaagtg gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat
    19981 caataacgaa ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc
    20041 cccactatcg gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc
    20101 gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg
    20161 tgtgccttcg acggtgcgcg gcagttttgc cggcacggcg caggttttcc aggagacgat
    20221 gaactcgcag gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat
    20281 cctttacgag agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt
    20341 tggagcgctg ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg
    20401 gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct
    20461 tgaagcccaa cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct
    20521 gcgttttcgc ccgattatga tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt
    20581 attgtcgggc ggtgacggct cggagctgcg gcaacccctg gagatcacca ttgtcggcgg
    20641 tctggtgatg agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga
    20701 ccgtctgcgg ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga
    20761 tcttcccgac agcacccgct ggcaattatg gattgtggct ttcggcttct ttatgcagtc
    20821 gctggacacc accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag
    20881 tccgttgcat atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc
    20941 cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt
    21001 gctgtttact ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct
    21061 ggcacgcgcg ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt
    21121 gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg
    21181 tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg
    21241 gcactggatc tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat
    21301 gttaatgccg aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct
    21361 ggcggttggc atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc
    21421 gccgctggcg attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct
    21481 gcacgccaga aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt
    21541 ttcgctgggc ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat
    21601 gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat
    21661 gatcccgatg gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg
    21721 ctttggttat cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt
    21781 gtttatgact accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca
    21841 agggatggtc aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc
    21901 ggacaatctg gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag
    21961 tatcggcgtc accatcgccg ggctgttgct ggggcttttt ggttcgcagc atatcagcgt
    22021 cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat
    22081 cgcccttccg gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat
    22141 ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg
    22201 tttctggcga ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt
    22261 atcagttttg agcgtggctt tattgattac atcaaacacg gcaatgaaca gcgattacag
    22321 ctgttaagtg atgcgcttgg cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc
    22381 aacaacgatc gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat
    22441 aaacccggcc cgggtatgcc gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa
    22501 aacaacaaag tactggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc
    22561 attctggtca acggtgcgga agtgggcgcg gtgattgcct cccctgttga gcggctgacc
    22621 cgcaatactg atatcaattt cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc
    22681 ctggcaacgt tactcgcggc gctcgccacc tttctgctgg cgcgcggttt gctggcaccg
    22741 gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta
    22801 acgcccacca gtgaagatga actgggcaaa ctggcgcaag acttcaacca gctcgccagc
    22861 acgctggaga aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg
    22921 cgtacgccat tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa
    22981 ttcacgccgg agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt
    23041 gacgatcttc atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg
    23101 gtagatttga tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc
    23161 cgtggcctga aactgcaatt ttccctgcca gacagtatca ccgtatttgg cgatcgcgac
    23221 cgtttaatgc agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc
    23281 ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac
    23341 agtgcgccag gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc
    23401 gaaggttccc gcaaccgcgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac
    23461 attgttgaag cccataatgg ccgcattatt gccgcccatt cgccttttgg cggggtaagc
    23521 attacagtag agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa
    23581 tcgacgaaaa cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc
    23641 tcattgatta tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg
    23701 tactggcgta tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg
    23761 gcaccgatgg cctgacatta tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga
    23821 tggtgacggc aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg
    23881 actacatctg caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc
    23941 gccgttgtaa accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgattatcg
    24001 acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat
    24061 ttcgtctgct taaaacgctc tctcacgaac caggaaaagt gttctcccgc gagcaattgc
    24121 tcaatcatct ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta
    24181 aaaatctgcg ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt
    24241 acggcgttgg ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt
    24301 actttgttag ccggagatgg cgttcagacc cgatccggca acattattcc acgcatggtc
    24361 agcaaactga cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc
    24421 agtaaacagc acctttagat gctgttttcg ataagcaatt tcaatcaagg agtcattatg
    24481 gctggttggt ttgaactcag taagagcagt gataatcagt tccggtttgt gctaaaagcg
    24541 ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag
    24601 ggcatcgcgt cggtgcgtag caacagcccg caagaagagc gctatgagaa aaaaacggca
    24661 agcaacggca aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc
    24721 cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc
    24781 acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc
    24841 cggcgcttta ccagatcccc cctacagcgc tacaatgccc gcccttaaag tgggggcctc
    24901 ccctaaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt
    24961 ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt
    25021 atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg
    25081 aattcaacca cgaaaatctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt
    25141 tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc
    25201 tgaaaccggt ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta
    25261 tgctggtgcg tgagcacttc cctgaaatgc cgatccacct ctcggtgcag gctaacgccg
    25321 tgaactgggc gacggtgaaa ttctggcagc aaatgggact gacccgcgtg atcctctctc
    25381 gcgagctgtc gctggaagag attgaagaga tccataatca ggtgccggat atggagatcg
    25441 agatcttcgt tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct
    25501 atatcaacaa gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca
    25561 acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc
    25621 cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga
    25681 tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt
    25741 acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg
    25801 gcgtgcattc gctgaaaatc gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg
    25861 cacaggttta ccgcaaagct atcgatgacg ccgctgcggg aaaaccgttc gataccagcc
    25921 tgctggaaac tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc
    25981 atactcacga cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt
    26041 ttgttggtga gtttaccggt gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata
    26101 aattctccgt tggcgacagt cttgagctga tgacgccgca aggcaacatt aactttaccc
    26161 ttgagcacat ggaaaacgcc aaaggcgaag ctatgccggt cgcaccaggc gatggttata
    26221 ctgtgtggct cccggtcccg caggatcttg agctaaatta cgcgctgcta atgcgtaatt
    26281 tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg
    26341 gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt
    26401 tcgctgaaaa acataatcca taaaatgcta gctgtaccag gaaccacctc cttagcctgt
    26461 gtaatctccc ttacacgggc ttattttttg tacataagct gtaaagaagg attgatccct
    26521 gataaccgtc ttccattgac cacatcgata gaatccccct tcatagcaca atgcctttca
    26581 cgtaacggta tcgtgctcgc ccaggttccg gctacgcaca gccagaacgc gcatgtttga
    26641 cgcttacaaa aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta
    26701 atcctgcacc ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga
    26761 aatgggctgc atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag
    26821 aagcttcggc ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg
    26881 caagaccaaa tttaccgata cttgtttcat cattatgcac agcacaacca gatataagaa
    26941 aaatactaat taatgatata gcagctatct ttttcatctc accttccccc attaaatacc
    27001 aacgacactc tctagtgttt aaatataata atg
//
LOCUS       DAZPEU010000035        26640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPEU010000035 DAZPEU010000000
VERSION     DAZPEU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.035"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            229..336
                     /locus_tag="ACHU2L_001379"
     CDS             229..336
                     /locus_tag="ACHU2L_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1356308.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            712..819
                     /locus_tag="ACHU2L_001380"
     CDS             712..819
                     /locus_tag="ACHU2L_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1356309.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="ACHU2L_001381"
     CDS             complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="ACHU2L_001381"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR1356310.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="ACHU2L_001382"
     CDS             complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="ACHU2L_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR1356311.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="ACHU2L_001383"
     CDS             complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="ACHU2L_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR1356312.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4614..4802
                     /gene="bcsR"
                     /locus_tag="ACHU2L_001384"
     CDS             4614..4802
                     /gene="bcsR"
                     /locus_tag="ACHU2L_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR1356313.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4814..5566
                     /gene="bcsQ"
                     /locus_tag="ACHU2L_001385"
     CDS             4814..5566
                     /gene="bcsQ"
                     /locus_tag="ACHU2L_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR1356314.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5563..8180
                     /gene="bcsA"
                     /locus_tag="ACHU2L_001386"
                     /pseudo
     CDS             5563..8180
                     /gene="bcsA"
                     /locus_tag="ACHU2L_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8191..10530
                     /gene="bcsB"
                     /locus_tag="ACHU2L_001387"
     CDS             8191..10530
                     /gene="bcsB"
                     /locus_tag="ACHU2L_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR1356315.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGTPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10537..11643
                     /gene="bcsZ"
                     /locus_tag="ACHU2L_001388"
     CDS             10537..11643
                     /gene="bcsZ"
                     /locus_tag="ACHU2L_001388"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR1356316.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11838..12227
                     /locus_tag="ACHU2L_001389"
     CDS             11838..12227
                     /locus_tag="ACHU2L_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR1356317.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            12309..14297
                     /gene="hmsP"
                     /locus_tag="ACHU2L_001390"
     CDS             12309..14297
                     /gene="hmsP"
                     /locus_tag="ACHU2L_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR1356318.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            14486..15772
                     /gene="dctA"
                     /locus_tag="ACHU2L_001391"
     CDS             14486..15772
                     /gene="dctA"
                     /locus_tag="ACHU2L_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR1356319.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15993..17489
                     /locus_tag="ACHU2L_001392"
     CDS             15993..17489
                     /locus_tag="ACHU2L_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1356320.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(17585..18514)
                     /gene="kdgK"
                     /locus_tag="ACHU2L_001393"
     CDS             complement(17585..18514)
                     /gene="kdgK"
                     /locus_tag="ACHU2L_001393"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR1356321.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18746..19513
                     /gene="pdeH"
                     /locus_tag="ACHU2L_001394"
     CDS             18746..19513
                     /gene="pdeH"
                     /locus_tag="ACHU2L_001394"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR1356322.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            19583..21643
                     /locus_tag="ACHU2L_001395"
     CDS             19583..21643
                     /locus_tag="ACHU2L_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1356323.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21825..23147)
                     /locus_tag="ACHU2L_001396"
     CDS             complement(21825..23147)
                     /locus_tag="ACHU2L_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1356324.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(23558..24571)
                     /gene="yhjD"
                     /locus_tag="ACHU2L_001397"
     CDS             complement(23558..24571)
                     /gene="yhjD"
                     /locus_tag="ACHU2L_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR1356325.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(24620..25519)
                     /locus_tag="ACHU2L_001398"
     CDS             complement(24620..25519)
                     /locus_tag="ACHU2L_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1356326.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            26039..>26640
                     /locus_tag="ACHU2L_001399"
     CDS             26039..>26640
                     /locus_tag="ACHU2L_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR1356327.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6362 a         6684 c         7269 g         6325 t
ORIGIN      
        1 gtgacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aagggttccg
       61 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acatcacggg ggccattctt gaacctgaac
      181 aactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt
      421 aagaaaaatg caacgattgc acgcggataa tattctgcgg aatgcgcagc acagagtgaa
      481 gacgtgacgg attccggaac agaaaaacag tcgtagaatc agcggcaagc cggaaaggtt
      541 ccggtgaggc gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga
      601 aaacccccgc acgttgcagg tatgcacctg acaacatcac gggggctaat cttgaaccca
      661 aacaactcaa gaatagccgc gaaacgttgg cattacaaca caggcggcta tatgacgctc
      721 gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
      781 gccagtatga tcgtgaactg gctgaataag cggaagtaac gtgtcatgcg ggcgtcaggc
      841 tgccgtaatg gcaatttgcg cccggatcag gccgcagagg ttaaactctg cggccttttt
      901 cgtgcttact gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta cggtttatct
      961 tggtattgaa taactactgc atttgagttc tcggagaccg gtgctgtttg tggcaaccca
     1021 ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa tcttgccatc aagaacgcga
     1081 accaccagat cggagatagc caggaagctg ctcggttggt caatgacaat cggtgccccc
     1141 tgatgtggtg ccttcatgcc gaagaatttc accccaacgg ggacgtctgt gatagacggg
     1201 ctagggatat cacgtaggcc agatacctgc attctgtcgc ccttcagcgc gccgccgtgt
     1261 tccggcacca cgaccaccat cactttacga cccgatttct ccagttcagt aaagaaggcg
     1321 tccagttcat caaagaattt ctgcgcccgc gctttgtaat ccgctgtttt gctgaccccc
     1381 ggataatggt tgccgtcatg cagtggaagc gtgttgtaga acgtggcact acggctgttt
     1441 ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc
     1501 gaaccatcaa agcccagcaa aataaccggc agatttgttt gatccatcaa ttcagtctgc
     1561 atgccgccat tttcgcgaac ttctttcaaa aaaccgccga actggccgtt atgtcccatc
     1621 atcaggtgct gggtaaagcc cagtttcgaa aggttatcaa acagatagca gtcgttattt
     1681 gccggttgat acagattagt gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc
     1741 gccgccgggc cactgtagga ggtggcggag ttaaagttct tgaaatcaat atcgaaatgc
     1801 gaccacagtg gatgcgacat caacccggcg gcttctatat ccgaccagga aagcgaacag
     1861 atgttaatca ccagtagttc aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc
     1921 gatttacgtt tcgcctccgc gttatagaaa ttattcagcc aggcgttaag gttcgccgtt
     1981 gttggcggtg cagtttgtgc gggcatatca cccactaccg gtgcgccacc cgtcgccgca
     2041 acggttgccg ctgcgttacc acccgtcgtt gttacagtgg tcgtcggttg tccggctggc
     2101 cacaaggaga aacttggtcc cgccagggta agtacgttca gccatagcag tatggcaacc
     2161 acaaaaacgg taatgcgaat ccattgtgac aggaataacc aggccactaa taaaacaaaa
     2221 atggccccaa tcatctgcca gttaataaag cgtgtgacaa ggtcgattaa ataatcggta
     2281 ctgaaccccg ccacctgcga accctggctc attatgcttt ccgggccagg caaccagatg
     2341 tcatgccaga acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg
     2401 ctgtaacgcg gaatgggcat cagcagaaac gcggcaaaca ccaaattgag gagcgggtgg
     2461 aagttaagat atcccgccca caacaggcca aacttaacca gaaaatagaa gttccagccg
     2521 gaaaggccgc gccagtattg ccagagggaa gaaggcatgg cggtattttg cgtaaattga
     2581 gtcatttttt ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat
     2641 aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg
     2701 ccagatagcc cagcgggaaa aatatcagtg cgcaaacgat aataatttca atgatatcgc
     2761 tgatggtcat catgatgagc gctccacagc atcatctaac agtcgcatgg gttctggtat
     2821 tcgtcgccag tggcgaccat cgtgctcggc attgatgacc ggtttagaac tttgcgttaa
     2881 aggcagcggc attccccatt gttctggggc aagcaggcgc atctgcacca gctcggcact
     2941 gatttgatca tcttcaaacc agaccatacg gtttgagaaa atgtcgccag taggcaatgg
     3001 gaaaatatga ttcaacgcgg tatccagatc gttaatccga cagaatgaga gaaacagcac
     3061 cagccgatta ccgccaatgg tcatgatatc gccggtacgg ttagggcgac atagcgtcag
     3121 ggcttgctca acgcggatac ccggtaccgg acgtagggca accagaacgc ctttaccgtg
     3181 ggcaggcaag agagggttat tcatcatgtt gttgacggca ttacagaaca catcccactt
     3241 ctggaaacca cgcagtttga gcggctgggt cattgacagc aaggtagtga tatcttccgg
     3301 tacatagcga ctaaacttct gcccttgcac gctttcgatc atcgtcagac aacgggagag
     3361 tggcgcattc cacggaataa ccatatttgc accgcaggcc aataacaaac gttcatcggt
     3421 ggcgcgcagg ctagcggtat tttcccgcac gaggattttc atcgcactac cgcgctggcg
     3481 acgcagggta tgaatgctgc gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa
     3541 gaccaccgtc gccgcctgag cggtacgggc ttcattgaac aggacttcgt tattgttgaa
     3601 cagttgccag tgttccgata gcggcggcgc accttccagt acagcaacat tactcagaat
     3661 gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg ctttgaacta atgtccagat
     3721 accattttgt tgctgaacgc taagctgctg acgggcgctg accccttttt cgttgcacca
     3781 gaaggcaata tccagcaaat gttggtcacc ctgaaaacgc aaactggcaa gaccaaaaag
     3841 tgaacggtat tcctcaagca acaatgaaaa ttgtttatcg ttattatttc cgggattaat
     3901 taccaaaagc gaacaatggt ttaacctgct ccatttattc attttatcca accatgagcg
     3961 aagccgctcg gcaggaatat tttgccatgc gttatttgcg caaacaagaa taaaaaggta
     4021 attatgggga tcaatagaac actgcaaatc acgggccaaa tagtatagac ctttttcatg
     4081 atttagcatt gaaaataatt ttattttttc cggcccttga gaatcatcta attgaaagat
     4141 tttcgccgga tcgctgtcca tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc
     4201 aattgtttga ttcgccagac tgatagcatc ttcatggcga tcgacgttaa accaccagac
     4261 gccgcctgct ggcatatgtc gcagctcatc ccataatgat gagataccga tagagaatac
     4321 agggtccaca atgtccctca tgtcaccatt ttgtctgatt atcagtttac tagcgaaagc
     4381 taagaaataa acctaccatt gaaattaaaa cttatcagat ttagcatgtg aatcaattta
     4441 gaaggtcaca agacgtgtga ttttagtctt ttcgttttta actttttctg ttgcgtaacg
     4501 gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac aataaaccgg cgaaatgctc
     4561 acggttagaa ttgtctcatg aatggtacgg ttatttcata gggatcaagc aaaatgaata
     4621 acaatgaacc agatactctg cctgatcccg cgataggcta tatcttccag aatgatattg
     4681 tggcgttaaa gcaggcattt tcactgcctg atattgatta tgccgatatt tcccaacgcg
     4741 aacagttggc cgcggcatta aaacgctggc cgttgctggc agagtttgcg caacaaaagt
     4801 aggggattgg tgaatggccg tactgggatt gcagggggtg cggggaggcg tggggacaac
     4861 aaccatcacc gccgcattag cctggtcatt acaaatgttg ggagaaaatg tcctggtggt
     4921 cgatgcctgc ccggacaact tgttgcgcct gtcatttaat gttgatttta cccaccgtca
     4981 gggctgggcc agagcgatgc tggatggcca ggactggcgt gacgctgggt tgcgctacac
     5041 ctcgcagctc gatttgctgc cttttggtca gttatccatt gaagaacaag aaaatccaca
     5101 gcactggcaa acccggctga gcgatatttg ctccggctta cagcaactaa aagccagcgg
     5161 gcgttaccag tggattttaa tcgacttacc gcgtgatgcc tcgcagataa cccaccagct
     5221 gctgagtttg tgcgagcact cgctggcaat cgtcaatgtg gatgccaact gccatatccg
     5281 actgcatcag caagcgctgc cggatggcgc acatattttg attaatgact tccgtattgg
     5341 cagtcaggtt caggacgata tttaccagct ttggttgcaa agccagcgcc gattactgcc
     5401 gatgctcatt catcgtgatg aagcgatggc tgaatgcctg gcggctaagc aaccagtagg
     5461 tgaatatcgc agtgatgcgc tggcggctga agagatactg acgctggcga actggtgcct
     5521 gttgaactac tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg
     5581 tggttgctta tcccgccggt caacgcgcgg cttatcgggc gttatcgcga ttatcgtcgt
     5641 cacggtgcgt cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt
     5701 tttattccgc tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat
     5761 acgcatatca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     5821 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     5881 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     5941 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     6001 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     6061 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     6121 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     6181 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     6241 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     6301 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     6361 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     6421 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     6481 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     6541 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     6601 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     6661 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     6721 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     6781 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     6841 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     6901 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     6961 gtggctatac ctccgcgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     7021 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtataa atcttccgtc
     7081 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     7141 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     7201 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     7261 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     7321 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     7381 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     7441 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     7501 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     7561 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     7621 cggtatcggt agaaagcaaa caggtacgcc gatcgaaccg cgtggagatg acgatgcccg
     7681 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     7741 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     7801 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     7861 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     7921 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     7981 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     8041 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     8101 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     8161 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     8221 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     8281 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     8341 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     8401 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     8461 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     8521 gcgatgctca acttcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     8581 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     8641 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     8701 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     8761 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     8821 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     8881 ttgccgatgg tctttgcggg tacgccggat gttgggctgc aacaagcatc tgccattgtc
     8941 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     9001 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     9061 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     9121 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     9181 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     9241 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     9301 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     9361 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     9421 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     9481 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     9541 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     9601 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     9661 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     9721 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat cccgatgccg
     9781 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     9841 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     9901 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     9961 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
    10021 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
    10081 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
    10141 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
    10201 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
    10261 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
    10321 atccgcgagt ccggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
    10381 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
    10441 gcgactatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
    10501 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
    10561 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
    10621 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
    10681 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
    10741 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
    10801 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
    10861 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
    10921 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
    10981 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
    11041 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
    11101 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
    11161 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
    11221 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
    11281 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
    11341 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
    11401 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
    11461 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
    11521 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
    11581 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
    11641 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
    11701 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
    11761 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
    11821 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
    11881 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
    11941 actggcgctg ggccccggca gcgtatggat tacttaccga ctggcaggaa gaagctgcgc
    12001 ggcaatccaa cgatggcggc agcagtcagg gcttcggcta cacggcgcgg gcattacttg
    12061 aacgacgtgt tacttccaac tggtttgttg gcacggcaat tgatatccag caggtgaaag
    12121 attacgcacc cagccatttc ctgctctacg tacgttattc cgccgccgga tggcagggtg
    12181 acatggattt accgccgcag ccgctgatac cttacgccga ctggtaagtt ttcagatagc
    12241 gcctctctta atgccgctgc gatcgggtat actcgggcgg caatctggga tttccggggg
    12301 gagacaattt gcgcgtaagt cgctcgttaa caatcaagca gatggcaatg gtggcagccg
    12361 ttgtcctggt gttcgttttt attttttgca ccgttttgct gttccatctg gtccagcaga
    12421 atcgctataa cacggctacg caactggaaa gcattgctcg ctctgtccgc gaacccttat
    12481 cttcagctat tttgaaaggc gatattcccg aagcggaagc tattcttgcc agcattaaac
    12541 cggcaggcgt ggtcagccgt gccgatgtag tgctgcctaa ccagttccag gcgctgcgta
    12601 aaagttttat tccagagcgt ccggtgccgg taatggttac tcgcctgttt gagctaccgg
    12661 ttcaaatctc gctgggcgtc tactcgctcg aacgtccggc aaatccgcag ccaatagcct
    12721 atctggtgct acaggcggat tccttccgta tgtataagtt cgtgatgagc actctctcaa
    12781 cgttagtgac catttactta cttttgtcgc ttatcctgac cgtcgccatc agctggtgca
    12841 ttaaccgcct gattttgcat ccgttacgca atattgctcg cgaacttaac gccatcccag
    12901 cccaggagct tgttggtcac caactggcat taccgcgtct gcatcaggac gatgaaatcg
    12961 gtatgttggt gcgcagttac aacctcaacc agcaattgct gcagcgccat tatgaagaac
    13021 agaacgaaaa tgcgatgcgc ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg
    13081 agatgctgga gcaggttgtc gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg
    13141 aaaccctgcg tgatactgcg ggcgtgctga aagaggcgca acgagaaatt ctgctgctga
    13201 cgctggtgga aaaactcaaa tcggtactgt cgccacgtat gatcctcgcg cagattagcg
    13261 gttatgactt tgctgtcatt gccaacggtg tacaggaacc gtggcacgca atcactttgg
    13321 gtcagcaagt actcactatc atgagcgagc gcctgccgat tgaacgtatt caactccgtc
    13381 cgcactgtag cattggcgtg gcgatgttct acggcgatct caccgccgaa cagctttaca
    13441 gtcgcgctat ttctgcggca tttaccgctc gccataaagg caagaatcag attcagttct
    13501 ttgatccgca gcagatggaa gcagcccaga agcggttgac ggaagagagc gatatcctta
    13561 atgcactgga aaatcatcag tttgcaattt ggttacagcc acaggtcgag atgaccagcg
    13621 gtaaactggt cagtgcggaa gtgttactgc gtatccagca accggatggc agttgggacc
    13681 tgccggatgg cttaatcgat cgcattgagt gctgtgggct gatggttacc gtcggtcact
    13741 gggtgctgga agagtcctgt cgattgcttg cagcctggca agagcgcggc attatgctgc
    13801 ccttgtcggt aaacctctct gcgctgcaac tgatgcaccc gaatatggtg gcggatatgc
    13861 tggaactgtt aacccgctat cgcattcagc cgggaacact gattctggaa gtgacagaaa
    13921 gccgacgtat tgacgaccct catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg
    13981 gagttcgggt ggcgctggat gatttcggca tgggctacgc agggctgcgt cagctgcagc
    14041 atatgaaatc gttgccaatc gacgtactga aaatcgacaa aatgtttgtt gaaggcttgc
    14101 cggaagatag cagcatgatt gctgcaatta tcatgctggc gcagagcctg aacttacaaa
    14161 tgattgccga aggcgtggag actgaagcac aacgagactg gctggcaaaa gcgggcgttg
    14221 gtattgccca gggcttcctt tttgctcgcc cactccctat tgaaatcttc gaagagagtt
    14281 acctggaaga aaagtagcta ccccaaactg attacaaaac tttaaaaagt gctggtttgt
    14341 gcgagccagc tcaaactttt taaccttttt gtttcaatta tgatccaggt acatttctgt
    14401 gatgttgtct gggtgttatt ttaaggccgc aggtaccccc cccccataac cttacaagac
    14461 ctgtggtttt actaaaggac accctatgaa aacctctctg tttaaaagcc tttactttca
    14521 ggtcctgaca gcgatagcca ttggtattct ccttggccat ttctatcctg aaataggcga
    14581 gcaaatgaaa ccgcttggcg acggcttcgt taagctcatt aagatgatca tcgctcctgt
    14641 catcttttgt accgtcgtaa cgggcattgc gggcatggaa agcatgaagg cggtcggtcg
    14701 taccggcgca gtcgcactgc tttactttga aattgtcagt accatcgcgc tgattattgg
    14761 tcttatcatc gttaacgtcg tgcagcctgg tgccggaatg aacgtcgatc cggcaacgct
    14821 tgatgcgaaa gcggtagcgg tttacgccga tcaggcgaaa gaccagggca ttgtcgcctt
    14881 cattatggat gtcatcccgg cgagcgtcat tggcgcattt gccagcggta acattctgca
    14941 ggtgctgctg tttgccgtac tgtttggttt tgcgctccac cgtctgggca gcaaaggcca
    15001 actgattttt aacgtgatcg aaagtttctc gcaggtcatc ttcggcatca tcaatatgat
    15061 catgcgtctg gcacctattg gtgcgctcgg ggcaatggcg tttaccatcg gtaaatacgg
    15121 cgtcggcaca ctggtgcaac tggggcagct gattatctgt ttctacatta cctgtatcct
    15181 gtttgtggtg ctggtattgg gttcaatcgc taaagcgact ggtttcagta tcttcaaatt
    15241 tatccgctac atccgtgaag aactgctgat tgtactgggg acttcatctt ccgagtcggc
    15301 gctgccgcgt atgctcgaca agatggagaa actcggctgc cgtaaatcgg tggtggggct
    15361 ggtcatcccg acaggctact cgtttaacct tgatggcaca tcgatatacc tgacaatggc
    15421 ggcggtgttt atcgcccagg ccactaacag tcagatggat atcgtccacc aaatcacgct
    15481 gttaatcgtg ttgctgcttt cttctaaagg ggcggcaggg gtaacgggta gtggctttat
    15541 cgtgctggcg gcgacgctct ctgcggtggg ccatttgccg gtagcgggtc tggcgctgat
    15601 cctcggtatc gaccgcttta tgtcagaagc tcgtgcgcta actaacctgg tcggtaacgg
    15661 cgtagcgacc attgtcgttg ctaagtgggt gaaagaactg gaccacaaaa aactggacga
    15721 tgtgctgaat aatcgtgcgc cggatggcaa aacgcacgaa ttatcctctt aatctcacca
    15781 cttatgcccg cactcccttc cccgagtgcg ggtgtttgcg cataattgac ccctgtgcct
    15841 gaacttacca cctgggtgag ttcaggttta tttcacttct tccgtgacat tttcatgttc
    15901 ttgcggtcta acacgaagtg tttttaacgt catttagact gaccgacaac gggcagtttt
    15961 tttattacca ggatagttga tcaggggttc acatgcaggg cataaaaatt cgacttttag
    16021 cgggcggttt gctgatgatg gccactgctg gctatgtgca ggcagatgcg ctccagcctg
    16081 atccagcatg gcaacagggg acgctttcca acggtttaca gtggcaagtg ctgaccaccc
    16141 cccagcgtcc cagcgatcgt gttgaaattc gcctgctggt taataccggt tcgctcgccg
    16201 aaagtacaca acagagcggt tacagtcacg ccatccctcg tattgcgcta acgcaaagcg
    16261 gtggccttga cgcagcacag gcgcgttcat tgtggcagca ggggatcgac cctaaacgcc
    16321 cgatgccgcc ggtaattgtc tcttatgaca ccacgctgtt taatctgagt ttgcccaata
    16381 accgtaacga tttgctgaaa gaagcgctct cttatctggc aaatgccact ggcaaattga
    16441 ccatcacacc agaaaccatc aaccacgcgc tgcaaagtca ggacatggtg gcaacctggc
    16501 ctgccgatac taaagagggc tggtggcgct atcgtctgaa agggtcaacc ttgttaggtc
    16561 acgatcctgc cgatccgctg aaacaacccg ttgaagcgga aaaaattaaa gatttctatc
    16621 agaaatggta caccccggat gcaatgacgc tactggtggt gggaaacgtg gatgcgcgct
    16681 cggttgtcga ccaaatcaac aaaacgtttg gcgaactgaa aggcaaacgt gaaacgccag
    16741 ctccggtgcc gacgctttct ccgctgcgtg cggaagcggt gagtattatg actgacgcgg
    16801 tgcgtcagga ccggttatct atcatgtggg atacgccgtg gcagccgatt cgtgaatcag
    16861 ccgcactgct gcgctactgg cgtgcggacc tggcccgcga ggcgctgttc tggcatgttc
    16921 agcaagcgtt aagcgccagt gacagcaaag acatcggtct tggatttgac tgccgtgtgc
    16981 tgtatctgcg tgcgcagtgt gccatcaaca tcgaatcacc aaacgacaag ctgaacagca
    17041 accttaatct ggtggcgcgt gaactggcga aggttcgcga taaaggtctg ccggaagaag
    17101 agttcaatgc gttagtggcg caaaagaaac tggagctgca gaaactgttt gccgcctatg
    17161 cacgggctga taccgatatt ctgatgggcc agcggatgcg ttcgttgcaa aatcaggtcg
    17221 tcgatatcgc gccggagcag tatcagaaac tgcggcagga tttccttaac agcctgactg
    17281 tggagatgtt aaatcaggat ctgcgtcagc agttgtcgaa tgatatggcg ttaatactgc
    17341 tgcagccgaa aggcgagccg gaatttaaca tgaaagcgtt gcaggcggcc tgggatcaaa
    17401 tcatggcccc atctaccgcc gctgcgacca cctctgtcgc cacggatgac gtacatcctg
    17461 aagtgacgga tattccacct gcacagtaat gcctgatggt gatgagttag cacaaccatc
    17521 aggtatttat ggatgagctg gatagagggg taacacttta tccagccttt tgcatatgct
    17581 gcgtttacgc tggcatcgcc tcacgcggga taatcgcgcc gcgatactga ataacggtac
    17641 ttgcggtcag gtgcccgcgt ttcgccgcgt tttccgcact gccgcccgtc aaacgtaccg
    17701 ccagataacc ggcgctgaaa gagtcgccag ctgcggtggt atcgatcact ttttctttcg
    17761 gcagtttcac cgccggaaca tcaactaacg cttcaccagc aatggacacc aggcaagaat
    17821 ccgccccgcg tttcaccacc acttctttca cgcccgcgtt atgggtgcgc gcaatgacgt
    17881 cttccaccgg ctgttgaccc cacagcgcgt cttcgtcgtc cagcgtcagg aaggcgatat
    17941 ccgtgcattc cagcatttgc tggtacacct gctgtgtctc ttctttgctg gcccacaggc
    18001 gcggacgata gttattgtcg aaaatcactt ttccgccttt ggcgcggcat tcgcgcagca
    18061 gggaaagcag cttttcgcgg ctggtcgggc ttaagatcgc caggctaatc ccgctcaggt
    18121 agagataatc gaaattcgcc agctcttcgc aaatcgccgc agactgctcg ctcgccagcc
    18181 agaatttggc ggcggcttcg ttacgccagt agtagaacgt acgttcgccg gtgctgtcgg
    18241 tttcaatgta gtaaaggccc ggaagacggt tttccatccg ttgggtcagg gaagtatcaa
    18301 cgttctcgcc gtgccaggcg tccagcatct gctggctaaa actgtccgtt cccagcgccg
    18361 ttacgtaatg aacggttaat gccgcaggat cgacctgacg ggcgatatag acggaagtgt
    18421 tcagggtatc gccgccgaaa ccgcgcttaa cgtccgcgcc tttctcggaa agctcaatca
    18481 tgcattcgcc aatcacggca atctttttgg acatagtcgt gaacctgatc tgtaaaaaat
    18541 tagcgttagt ttgcgctgtg gtggtttgct ggtcaaccat attaaaacag tgttccatta
    18601 tttttttgag ccagaacgta ttcctgaaag attccgttgt ggagtgagga aatttacccc
    18661 ggccaggcgg taaagttctg cccttacgcg ccgataatct ttgtcgagtc cgggcagcat
    18721 cacttttaaa cacaggacat ctttgatgat aaggcaggtt atccagcgaa taagcaaccc
    18781 tgaagcaagc atcgagagct tgcaggaacg gcgtttttgg ttgcagtgtg agcgtgctta
    18841 cacctggcag ccgatctatc aaacatgcgg gcggttaatg gccgtggagc tattaacggt
    18901 ggtcacgcat cccttgaacc cttcgcaacg cctgccgccg gatcgctatt ttactgaaat
    18961 caccgtcagc catcggatgg aggttgtgaa agagcagatt gatttgctgg cgcaaaaagc
    19021 cgacttcttt gtagagcacg gcctgctggc atcggtcaat attgatggcc ctacgctcat
    19081 cgccctgcgt cagcaaccaa aaatcctgcg ccagattgag cgtcttccct ggctgcgttt
    19141 cgaactggtg gagcatatcc gtctgccgaa agattcaacc tttgcctcga tgtgtgaatt
    19201 tggcccgctg tggctggatg attttggtac cgggatggca aatttctctg cactaagtga
    19261 agtgcgttat gactacatca aaatcgcgcg agaactgttt gtgatgctgc gtcagtcgcc
    19321 ggaaggccgc acactctttt ctcagctttt acatctaatg aatcgctatt gtcgcggggt
    19381 gattgtcgag ggcgtagaaa caccggaaga gtggcgtgat gttcagaact cgcccgcatt
    19441 cgccgcacaa ggctggtttc tttcacgccc ggcaccgatg gaaacgctga atacggcggt
    19501 tctggcgcta taagctgcct cattttccgc cgggctggac tatctttagg actggcacag
    19561 gaagaatatg aggaagcgaa cgatgagcaa ggcaggcaaa ataaccgctg cgatttcagg
    19621 ggctttcttg ttgttgattg tcgtggcgat cattttgatt gcaacttttg actggaatcg
    19681 actcaaaccg accatcaacc agaaagtctc tgcggagttg aatcgtccgt tcgctatccg
    19741 tggcaatctg ggcgtggtgt gggagcggca aaaacaagaa actggctggc gcagctgggt
    19801 gccgtggcct catgtacatg cagaagacat cattcttggt aatccaccgg atattcccga
    19861 agtcacgatg gtgcatttgc cgcgagtaga ggcaacgctg gcaccgctgg cgctgctgac
    19921 caaaacggtc tggctgccgt ggatcaagct cgaaaagccc gacgcgcgcc tgattcgcct
    19981 ctctgaaaag aacaataact ggacgtttaa tcttgccaac gatgacaaca aagacgcgaa
    20041 tgcaaagccg tcggcatggt cgtttcggct ggataatatt cttttcgatc aagggcggat
    20101 cgccattgat gacaaagtaa gcaaagcgga tctggaaatt tttgttgatc cgttaggcaa
    20161 gccgctgcca ttcagcgaag ttactggatc gaaaggtaaa gcggataaag aaaaggtggg
    20221 cgattacatt tttggcctga aggcgcaggg acgttataac ggtgaaccgc tcacgggtac
    20281 gggaaaaata ggcggtatgc tggcgctgcg tggcgaaggg acgccgtttc cggtacaggc
    20341 tgatttccgt tcaggtaata cccgtgttgc ttttgatggc gtcgtgaatg acccaatgaa
    20401 gatgggcggt gtcgatttac ggcttaaatt ttctggcgat tcactgggtg atctctatga
    20461 actgacgggc gttctgctgc ccgatacccc gccgtttgaa acggatggtc ggctggtagc
    20521 gaaaatcgac actgaaaaat cgtcggtctt tgattatcgc ggttttaatg ggcgaattgg
    20581 cgatagcgat atccacggtt ctctggtcta caccaccgga aagccacgac caaaactgga
    20641 aggtgatgtc gagtcgcggc aattgcggct ggcggacctg ggaccgttga ttggcgttga
    20701 ttccgggaaa ggggcagaaa agtcgaaacg gtctgaacag aagaagggcg aaaaaagcgt
    20761 tcagcctgcg ggcaaagtgc tgccttatga ccgcttcgaa accgataaat gggacgttat
    20821 ggatgccgat gttcgcttca aagggcggcg cattgagcat ggcagtagcc tgccgattag
    20881 cgatctttct actcatatca tcctcaaaaa tgctgacctg cgcctgcaac cgctgaaatt
    20941 tggcatggcg ggcggcagca ttgcggcgaa tattcatctg gaaggcgata aaaagccgat
    21001 gcaggggcgg gcagatattc aggctcgtcg actgaaactg aaagaactga tgcccgatgt
    21061 ggaactgatg cagaagacgc tgggggaaat gaacggtgac gcggaactac gcggtagcgg
    21121 taactcggtg gcggcgcttt taggcaacag taacggcaac ctgaaactgt tgatgaatga
    21181 cgggctggtg agccgcaacc tgatggagat tgttgggctg aatgtcggca actacattgt
    21241 cggtgcgata tttggtgacg atgaggtgcg ggtgaactgc gcggcggcga atctgaatat
    21301 tgccaacggc gtggcgcgcc cgcagatttt tgctttcgat actgagaacg cgttgattaa
    21361 tgttaccggc acggcaagtt ttgcttcgga acagctggat ttgactattg atccggagag
    21421 taaagggatt cggattatca cactgcgttc gccgctgtat gtgcgtggga cgtttaaaaa
    21481 tccgcaggct ggggtgaaag ccggaccgct gattgcccgt ggtgctgttg ctgcggcact
    21541 ggcaacgctg gtaacaccgg cggcggcgtt actggcactg atctcacctt ccgaagggga
    21601 ggctaatcag tgtcggacga tattgtcgca gatgaagaag tgatgataat tgccggatgt
    21661 ctgacatccg gcaattaaac agatttagat ttttggcaaa gtgtgctttg ttcatgccgg
    21721 atgcggcgtg aacgccttat ccggcctaca aatccttgtg aattcaatat attgtatgga
    21781 aattcagtcc tgataagcgt agcgcatcag gcttttcaca cttattacaa cgactgatgt
    21841 cgcgtctcat gggtcagcag cagggcgatt aacgtcaagc cagccatcgc cgccagatat
    21901 aaccccaccg cacctaaccc gtagttagcc tgcaaccagg ctgcgatata cggcgcaacg
    21961 gaagccccga gaatcgacga tacgttgtaa gagaacgatg ctccggtgta acgcacttct
    22021 gtcggaaaca gctctggtaa cagcgcaccc atcggcccga aggtcagccc catcagactt
    22081 aaccccagca gcaggaaggc aaaaaccaga atcgggttgc cagaaccgag cagcgggtta
    22141 aaggcgaaca gcgcgaacag gatgatcagc gtggtgatga tcaccatgct tttacggcga
    22201 ccaaaggcat cagccagtaa tccggcgact ggcaccatca cgccaaaacc aataactgcc
    22261 atcatcagca tccacaacac ttcgttacgc ggcaggccaa gcccaactgg cgcggcggcg
    22321 gtactgaagg tcatagagta gaccgtcatg atgtaaaaca gcgtataggt tgccagcata
    22381 atgaacgtac ccagtacggt tacgcgaaca tgtttggtca gcagcgtacc cagcgggatc
    22441 ttcacctgtt ttttcgcttt agcgactttc tcaaacaccg gcgactcatg cagcgacacg
    22501 cgaacataca ggccgataat gaccagcacc gccgagaaga taaatggcac acgccagccc
    22561 cagctcataa actgctcatc ggtcagtagc caggaaagca gcaagaaagt gccgttggca
    22621 aagaagaagc caatcggtgc gcctagctgt gggaaggagc catacagtgc acgtttgcgc
    22681 ggtggggcgt tttcagtcgc cagcagcgcc gcgccgcccc attcaccgcc taagcccaga
    22741 ccctgaccaa atcgagccag cgccagcagc agtggggcga aaataccaat cgtggcatag
    22801 cccggcagca gaccaatcac cacggtcgaa atccccatcg ttagcaacga ggcgaccagc
    22861 gtcgctttac gcccaacgcg atcgccaaaa tgaccaaaaa cggcagagcc aatggggcgc
    22921 gcgacgaagg cgatggcgaa ggtggcgagc gactgtagcg ttgctgccgt tggatcgccc
    22981 tgcgggaaga agatatgcgg aaacacaata acggccgcag tggcgtaaat gtaaaagtcg
    23041 aagaactcaa tggccgtgcc aatgagagag gcgacaagga ctttattacg cgagttgatc
    23101 ggcgtgtatt cttgttcgtg gtcgagtgtt gtggctgttg cttgcataat cttttcttat
    23161 tttttggcta acgaatagcc atattacgca cagcaaaagt gatatttcaa tgtgtaacaa
    23221 atcgcggaaa tggtcaaaaa ccaggcaaaa aggggataat gtttaggcca gtgatttttc
    23281 ttctatgaaa tgcttcacat aattttataa atagtggata aaactggttt caggttaaat
    23341 aaaagttacc aactggattt atatgctggg gttatgagtt ggagtgaaat tgtttgctcc
    23401 caaatagatg gctgaagagg ctctggggaa gagtttcaag aggttatgtg cgcattatag
    23461 ccggatgcga tgctggcgca tcttatccgg cctacgggga gccaggccga ataaaatcgt
    23521 atgccggata aggcgtttac gccgcatccg gcctatttta aggctgcgtt ttccctggca
    23581 ttcgcgggtc gtctttatat tcggcggtgg caatccacgc cgcgcaaaac agcgtcaaac
    23641 gggcgaagaa gtagaaaaat gccatcagcc ccagcacgga accaaatgct gcgccagagg
    23701 gggatttcat cagcgatggc agggtgtagg tcatcacgat tttaatcact tcaaaaccaa
    23761 tagcagccag aaatgttccg cgaatcaggg cttttttgcg cggacggtgg cgcggcaacc
    23821 gccagaagat ccagaagaaa agcagatagt tggcgaagat ggaaatcgcc aggccaatca
    23881 atcgccacgt cggtttcagc cactcaatgc tgttcaggtg cagggcgcta ataatcattt
    23941 gctgcgccga accggcaacc gaggtgatcg aaagcgtcac aatcagcgca atcaacaaac
    24001 caatcagtga aataaaatca cgcagatatt ttacccagaa cttctcctga tcttgcggcg
    24061 agcgttccca gacatcgcgc gactgggcac gaatcgcttc acgcaggtta cccatccagt
    24121 tgatgccgga ataaagtgcc accgccaggc cgacaagccc tacagtcgta cgctgctgaa
    24181 cggcggtgtt gatggtgttt ttcaacgtgg cggctagtgt cggatcgctg atgttttgca
    24241 gaattttgtc gaagatatcc tgtagcaaca tcggatggga ggccagcaca aagccccctg
    24301 cggcaaacga caccatcaaa atcgggatca tcgacaaaaa tgagaaatag gtgatagccg
    24361 caccaaactg gttacccagc cgatcgttaa agcgttctgt cgcgcgaata aggtgcgcaa
    24421 taaccggttg gcgttggact ttttcggcag tggtggtgac ggtttccagc gcctggctaa
    24481 ctttgccgcc tttctcgcta ttatccaact gcttaatcgg ctcgtgctcc agatcctggg
    24541 tgggacgttt gatctcgttt tcctgcgtca tccgctcttt tgtccttctg tcgtggtcat
    24601 tcttaaaaag tatagtcggt cagtccaggt gctctttcat tacgccagcc agccactgca
    24661 taaacaggtt tacacgccgg gaaagctccc gacgctgcgg gtaaaccagc gaaagggaga
    24721 gcggctcggc acggtagcct ggcaacactt caataagccg cccggcacgc agggcttcgc
    24781 gcacggcaat gcgcgggatc tgaataatcc ccagaccggc aagaccggcg gcgagatagt
    24841 tttcgctact gtttaccgtc aacatgccgc cagacttaaa ccactggacg ccattaacgc
    24901 tggcaacctc aaaacctaac ggatgtacac ccaggtgcgg tgtataacgc actattgcgt
    24961 gtgaagtcag atcatcgggg ctttgcggat aaccaaagcg cgtcagatag tgcggactgg
    25021 cacagttgac catggttagt ttgccgaggg gacgggcgat aacgccatct tccggtaacg
    25081 cgccagtgcg tatcacgcaa tcaaaaccat catgaagaat atctaccgga cggttatggc
    25141 tactcagttc cagctcaatt cccggatgct gatagagaaa ttccgacaga cgcggcagta
    25201 acaggttttt cgcgattcct gacgggatgt cgatgcgtaa tttaccgctg atactggtgg
    25261 catcctgttg aaacagaccg tccagttcgc tgagattact caacacatct tttgcccgtt
    25321 gataataggt catgccttct ggcgtgagtt tgacccgtcg cgtagtgcgc tggagaagct
    25381 gggtgccaag ttgatgttcc agtgcctgta tctggcgcga aacacttccc tttggcaaag
    25441 caaagaaatc cgccgcgcgg gaaaaacttt ccagctccgc gactttgatg aacaactgca
    25501 ttgcgtgaat tttatccatt tctttgaccg attgttgttc acagcgaaac agtaacgcgc
    25561 atatcgcatt gtttattgtt ttttctaaca tctaatgccg ggtttcacaa tgttgtcatg
    25621 acgaatgtgg cgatttctca gcaggaaaag ggcggaatag ctttatgaga aaggtgaatc
    25681 cccttaaaca gagcgtattt ttcagtgatt ccctttaaag tgaatagagg acgtattggc
    25741 tatttgtgct tatttttgtc tcattgccat ttgttatttt ggcttgtagt cgcggaattt
    25801 catttttaat tgcatgcgta ctaaccacca atataaggaa atcctcaaaa ttgttttaaa
    25861 tagtgccctc gacatgtaat ttcaggcgtt tagctgtaac tatttatatc ggtaactatt
    25921 ttctacgcgt catctctgta gaatggccac cgccaaatgg cgaattctta ttttttctcg
    25981 cgggtaatac ccttccacta cttaagtctt tttaacgtat tcaggtggag attcagttat
    26041 gcaaatagtc atgtttgaca ggcagtcaat atttattcat ggaataaaaa tcagtttaca
    26101 gcagcgtatt ccaggagtga gtattcaggg ggtcagtcag gcagacgagt tatggcaaaa
    26161 gctggaaagt taccctgaag ccttagttat gctcgatggc gatcaggatg gtgagttttg
    26221 ctactggttg ctgcaaaaaa ccgtggtgca gtttcctgag gttaaggtgt taatcacggc
    26281 gacggattgc aataaacggt ggttacagga agttatccat tttaatgtgc tggccattgt
    26341 gcctcgcgat tcaaccgtcg agacatttgc gttggcggta aatagtgcgg cgatggggat
    26401 gatgtttctg cctggagact ggcggacgac gccggaaaag gacattaaag atctcaaatc
    26461 gttaagcgcc cgccagcggg agattttaac catgttagcg gcgggcgaat caaataagga
    26521 gatcggcaga gcgctgaata tcagtaccgg aacggtaaaa gcgcatctgg aatccctgta
    26581 tcgccgtctg gaagtgaaaa atcgcaccca ggcggcaatg atgttaaata tctcctcctg
//
LOCUS       DAZPEU010000036        26540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPEU010000036 DAZPEU010000000
VERSION     DAZPEU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.036"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(82..486)
                     /locus_tag="ACHU2L_001400"
                     /pseudo
     CDS             complement(82..486)
                     /locus_tag="ACHU2L_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            complement(670..2088)
                     /gene="gltD"
                     /locus_tag="ACHU2L_001401"
     CDS             complement(670..2088)
                     /gene="gltD"
                     /locus_tag="ACHU2L_001401"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR1356328.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(2101..6561)
                     /gene="gltB"
                     /locus_tag="ACHU2L_001402"
     CDS             complement(2101..6561)
                     /gene="gltB"
                     /locus_tag="ACHU2L_001402"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR1356329.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            7236..8165
                     /locus_tag="ACHU2L_001403"
     CDS             7236..8165
                     /locus_tag="ACHU2L_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR1356330.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            8261..10597
                     /gene="arcB"
                     /locus_tag="ACHU2L_001404"
     CDS             8261..10597
                     /gene="arcB"
                     /locus_tag="ACHU2L_001404"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR1356331.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            10827..11480
                     /gene="elbB"
                     /locus_tag="ACHU2L_001405"
     CDS             10827..11480
                     /gene="elbB"
                     /locus_tag="ACHU2L_001405"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR1356332.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            11477..12204
                     /gene="mtgA"
                     /locus_tag="ACHU2L_001406"
                     /pseudo
     CDS             11477..12204
                     /gene="mtgA"
                     /locus_tag="ACHU2L_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(12201..12833)
                     /gene="yrbL"
                     /locus_tag="ACHU2L_001407"
     CDS             complement(12201..12833)
                     /gene="yrbL"
                     /locus_tag="ACHU2L_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR1356333.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(13046..13318)
                     /gene="npr"
                     /locus_tag="ACHU2L_001408"
     CDS             complement(13046..13318)
                     /gene="npr"
                     /locus_tag="ACHU2L_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR1356334.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(13315..14169)
                     /gene="rapZ"
                     /locus_tag="ACHU2L_001409"
     CDS             complement(13315..14169)
                     /gene="rapZ"
                     /locus_tag="ACHU2L_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR1356335.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(14215..14706)
                     /gene="ptsN"
                     /locus_tag="ACHU2L_001410"
     CDS             complement(14215..14706)
                     /gene="ptsN"
                     /locus_tag="ACHU2L_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR1356336.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(14824..15111)
                     /gene="hpf"
                     /locus_tag="ACHU2L_001411"
     CDS             complement(14824..15111)
                     /gene="hpf"
                     /locus_tag="ACHU2L_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR1356337.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(15134..16567)
                     /gene="rpoN"
                     /locus_tag="ACHU2L_001412"
     CDS             complement(15134..16567)
                     /gene="rpoN"
                     /locus_tag="ACHU2L_001412"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR1356338.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(16615..17340)
                     /gene="lptB"
                     /locus_tag="ACHU2L_001413"
     CDS             complement(16615..17340)
                     /gene="lptB"
                     /locus_tag="ACHU2L_001413"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR1356339.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(17347..17904)
                     /gene="lptA"
                     /locus_tag="ACHU2L_001414"
     CDS             complement(17347..17904)
                     /gene="lptA"
                     /locus_tag="ACHU2L_001414"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR1356340.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(17873..18448)
                     /gene="lptC"
                     /locus_tag="ACHU2L_001415"
     CDS             complement(17873..18448)
                     /gene="lptC"
                     /locus_tag="ACHU2L_001415"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR1356341.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(18445..19011)
                     /gene="kdsC"
                     /locus_tag="ACHU2L_001416"
     CDS             complement(18445..19011)
                     /gene="kdsC"
                     /locus_tag="ACHU2L_001416"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR1356342.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(19032..20018)
                     /gene="kdsD"
                     /locus_tag="ACHU2L_001417"
     CDS             complement(19032..20018)
                     /gene="kdsD"
                     /locus_tag="ACHU2L_001417"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR1356343.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(20032..20997)
                     /locus_tag="ACHU2L_001418"
     CDS             complement(20032..20997)
                     /locus_tag="ACHU2L_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR1356344.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            21207..22016
                     /gene="mlaF"
                     /locus_tag="ACHU2L_001419"
     CDS             21207..22016
                     /gene="mlaF"
                     /locus_tag="ACHU2L_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR1356345.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            22024..22806
                     /gene="mlaE"
                     /locus_tag="ACHU2L_001420"
     CDS             22024..22806
                     /gene="mlaE"
                     /locus_tag="ACHU2L_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR1356346.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            22811..23362
                     /gene="mlaD"
                     /locus_tag="ACHU2L_001421"
     CDS             22811..23362
                     /gene="mlaD"
                     /locus_tag="ACHU2L_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR1356347.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            23381..24016
                     /gene="mlaC"
                     /locus_tag="ACHU2L_001422"
     CDS             23381..24016
                     /gene="mlaC"
                     /locus_tag="ACHU2L_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR1356348.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            24016..24309
                     /gene="mlaB"
                     /locus_tag="ACHU2L_001423"
     CDS             24016..24309
                     /gene="mlaB"
                     /locus_tag="ACHU2L_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR1356349.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            24469..24723
                     /gene="ibaG"
                     /locus_tag="ACHU2L_001424"
     CDS             24469..24723
                     /gene="ibaG"
                     /locus_tag="ACHU2L_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR1356350.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            24778..26037
                     /gene="murA"
                     /locus_tag="ACHU2L_001425"
     CDS             24778..26037
                     /gene="murA"
                     /locus_tag="ACHU2L_001425"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR1356351.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(26085..26363)
                     /gene="sfsB"
                     /locus_tag="ACHU2L_001426"
     CDS             complement(26085..26363)
                     /gene="sfsB"
                     /locus_tag="ACHU2L_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR1356352.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
BASE COUNT         6147 a         6744 c         7125 g         6524 t
ORIGIN      
        1 cttcgtttat ttgcatgaat atacaaaccg aaaataggag atgcaataca aaagctgtgg
       61 aaatatcaaa cagcctctca atttggcaga cagcgtctgc caaatctttg ccgatgggaa
      121 ttctgcagat ggtgtctgcg gaattcccaa tggtgttaat tcgccagaca ctgtctggcg
      181 aatttcaaca tgttgaaaaa agaggtaaaa aagcctcatt tggcgtaagt tttttgcaga
      241 gaccccacgt ttataccgtc gacttaaatc cttcgatagc cgatcaatca actgtgcacc
      301 ataagctgct ctggcctcac caccttgttc aaattcgaca atccgtcggc caatttccca
      361 gtacgtcgcg gtcatcagcg cgttaacgct acgtaccgtt tccgttcggg cgctatcgac
      421 cagatgaata atgccgttgt ggatttgctg gtagcctgtt gaggttctct ctgagagcga
      481 ttccattatc tgtcccttta tatggcgctg cgttgaataa tggaatcagc tatacgccgg
      541 gcatctaccg gtaacaatcc agatgagcgt attacattgg ttttcttcag attatgcgag
      601 gcgatttcca gaaagaaagg agaatttcag cgccgactct tttctgagga ggcggcgctg
      661 ggacagagct taaacttcca gccagttcat aatgccgtct gccgctttgc gaccttcggc
      721 gatggcggta acgaccagat cggaaccacg gacgatatcg ccgccagcaa agattttcgg
      781 gttgctggtc tggaaagcgt tgtcgttgcc ttccggagcg atgatgcggc cttgtgaatc
      841 cagctcgacg ctgtgttttg ccagccattc catgttgtgt ggacggaaac caaacgccat
      901 gatcaccgca tctgccggaa cgatatgttc ggaacctgca acgatctccg cgcggcgacg
      961 gcctttggca tccggttcgc ccatttcggt acgtaccatt tttacgccgc tgactttgcc
     1021 gttaccgttc acttcaatcc ccagtggctg gacgttgaat ttgaactcta cgccttcttc
     1081 ccgcgcgttt ttcacttcgc ggcgggaacc cggcatgttc tcttcatcac gacgataggc
     1141 acaggtaacg tgctttgccc cctggcgtac agacgtgcgc acgcagtcca tcgcagtgtc
     1201 gccaccgcca agtactacca cgcgtttgcc ttccatgctg acgaacggtt cgtcgcgggt
     1261 ttcaccaaag cccattaact gtttggtgtt ggcgataagg aacggcagcg ctgcgtacac
     1321 gccatcggcg tcttcgtttt ccagcccgcc gcgcattgac tgataagtcc caacgccaag
     1381 gaacacggca tcgtaatcac tcagcagatc gtccagctgt acgtcgcggc ccacttcggt
     1441 attgagtttg aattcaatac ccatgccggt gaagatttca cggcgacgcg tcattacctc
     1501 tttttccagc ttgaaggccg gaataccgaa ggtcagcagc ccgccaattt ctggatgacg
     1561 gtcgaagaca acggctttta cgccgttacg cgtcaggaca tccgcacacg ccagacctgc
     1621 cgggcctgca ccgataatcg ccactttttt accggtttgc ttcacgccgg acatatccgg
     1681 acgccagccc atttcgaacg ctttatcatt gatataacgc tcaatgttgc cgatggtcac
     1741 cgcaccgaac tcatcgttca gggtgcagga accttcgcac agacggtctt gcgggcagac
     1801 acgtccgcat acttccggca gtgtgttggt ctgatgcgac agttctgccg cttcaaaaat
     1861 acgcccctcg ttggcgagct tcagccagtt tgggatgtag ttgtgtaccg ggcatttcca
     1921 ctcgcagtat gggttgccgc acgacagaca gcggtcagcc tgcgctttgg cctggccttc
     1981 ggaaaacggc tcgtaaattt caacaaactc aattttacgg atcttcagcg gtttctttgg
     2041 cggatcaacg cgctgcaggt cgataaattg ataaacattc tgactcattg ttgctacccc
     2101 ttactgcgcc tgcacgcgca actctgctgc gctacgacta cggtgaccca gcagtgcttt
     2161 tacatcactg gacttcggtt taaccagcgc gaatttcgtg gcgaaggttg accagttcgc
     2221 cagaatctct tcaccgcgct gagaaccggt atgctgaaca tgctcggtga taagaccgcg
     2281 cagatgctct tcatggatcg ccagatcgtc gacgcttaag acttcgacca gttccgggtt
     2341 aacgcgtttg cggaaatcac cgctttcatc gagaacgtaa gcgaagccgc cggtcatgcc
     2401 cgcgccgaag ttaacgccgg ttttacccag aatacagaca ataccgcccg tcatatattc
     2461 acagccgttg tcgccaatgc cttctaccac ggtgatagca ccggagttcc gcacgccgaa
     2521 acgttcaccc gcgcggcctg cggcatacag acgaccaccg gtcgcgccat acaggcaggt
     2581 gttgccgata atgcttgctt catggctgcg gaaggcggaa ccaaccggag gacgaatggc
     2641 gattaagccg cccgccatgc ctttaccgac atagtcgttg gcatccccgg tcaggtacag
     2701 ttccacgccg cccgcgttcc acacaccgaa actctggcct gcggtgccgt tgaagtacgc
     2761 tttgatagga tcggctgcca gcccctgatc gccgtgcgtc tgggcgatat agcctgaaag
     2821 cgacgcgccg acagaacggt cggtgttgcg gatatcgaac cagaaggttt tgctctggcg
     2881 ttcatcgaca aacggtttcg cctgttgcag caactgcgcg ttcagtaggc cgttatcaaa
     2941 cggcgggttg ttttcagtgc agtagagtga cttacctgca tgtggctcgg cagtctccag
     3001 cagcttcgac agcgccagtt tctgctgttt ggcggtgaaa ccgtccagct ctttcagcag
     3061 gtcggtgcga ccaatcagat ccaccagacg cgttactccc agctgtgcca tcagctcgcg
     3121 ggtttcacgg gcgataaact caaagtaatt agtcaccttg aacggcaggc cgtgatagtg
     3181 attcttacgc agtttgtcat cctgagttgc tacacccgtg gcgcagttgt tcaggtggca
     3241 aatacgcaga tatttacagc cgagcgccac cattgggcca gtgccgaagc cgaagctttc
     3301 tgcgccgaga atcgccgctt tgatgatatc gacacccgtt ttcaggccgc catcaacctg
     3361 caaacgaatt ttatgacgca agccgttggc aaccagcgcc tgttgggttt ccaccagacc
     3421 gagttcccac gggcagcctg catatttcac tgacgacagc gggcttgcgc cggtgccgcc
     3481 gtcatagccc gcgatggtga tcaagtccgc ataagctttc gccacgccag tcgcgatggt
     3541 gccaacgcct ggttcggaca ccagcttaac ggagatcatc gctttcgggt taacctgctt
     3601 gaggtcgaaa ataagctgcg ctaagtcttc gatagagtag atatcgtggt gcggcggcgg
     3661 ggagatcagc gtcactccgg gcaccgaata gcgcagtttg gcgatgtaag gagtgacttt
     3721 gtcgcccggc aactgaccac cttcgcccgg cttcgcgccc tgggcgactt taatctgaat
     3781 gacgtcggca ttgaccagat acgccggagt aacgccgaag cgaccagaag ccacctgctt
     3841 gatgcgcgac actttgttag tgccatagcg cgccgggtct tcgccgcctt caccggagtt
     3901 cgagttaccg ccgatgctat tcatcgcttc cgccagcgcc tcgtgggctt ccgggcttaa
     3961 cgcgccgata gacatcgcgg cggtatcaaa gcgtttaaac agttcgcttg ccggttcaac
     4021 atcagcaatg ttgaccgcgt tttcacccgg cgtaattgcc agcagatcgc gcagcgtggt
     4081 tgccggacgc tcattaacca gcttcgcgta ttcctgatag tcgctgtact cgccgctttg
     4141 taccgcctgt tgcagcgtgc gcaccacgtc cgggttgtag gcgtggtatt cgccgccgtg
     4201 gacgtatttc agcaggccgc cctggctaat cggcttgcgc gccagccagg cacgtttcga
     4261 cagattcagc agatcctgct ggaagtcttc aaagcttgcc ccaccaatgc ggctgaccgc
     4321 gccctggaag cacaggccca ctacatcatc gtgtagaccg accgcttcaa acagtttcga
     4381 gcagcggtaa gaggcgatgg tggagatgcc cattttggac atgattttgt acaagccttt
     4441 gttgatgccg ttacggtagt tgagcatcac ggtacgataa tctttggcaa tcgcgtgggt
     4501 gtctaccagg cggcccagcg tttcataggc aaggtacgga taaatagccg tcgcgccgaa
     4561 acccagcaac acggcgaagt ggtgcggatc gcgggcgctg gcggtttcga cgatgatgtt
     4621 ggcatcgcaa cgcaggcttt gatcgaccag acgggtctgg atcgcgccaa ccgccatcgg
     4681 agccggaacc ggcaggcgat ctttagcgat attccggtcg gagagcacca gcaacacggt
     4741 gccgctacgt accatttttt cggctttgtc gcatagctct ttgactgtcg cttcgagcgt
     4801 ggttttagtg acgtcaaagg tgatatccag cgtatctgcg cggtagtgct cctctttcat
     4861 cgtcgtgagc tgtttgaaat cggagtagag cagaatcggc gatttaaagc ttaaacggtg
     4921 cgcctggccc tctgcttcgc aaaagacgtt catttcacga ccgatactgg tggcgagcga
     4981 cataacatgc gcttcacgca gcgggtcgat tggcgggtta gtcacctggg caaactgctg
     5041 gcggaagtag tcgtaaataa tgcgcggctg actggagagc acggcgaatg gggtatcatc
     5101 gcccatcgaa ccgaccgctt cctgaccgtt ttcgcccagt acgcgaatta cggagtccag
     5161 ctcttccgcg ctgtagttaa actgtttctg gtagctggca agcgtgtcgt cgtccagttc
     5221 gcggctaccc acttcttcat cgggcagatc ttcaaacggt accagtcggc ggacgttttt
     5281 ctccatccac tctttataag ggtggcggct tttcagatcg tcatcggttt ctgccgagtg
     5341 cagaatacgc ccactgcgag tgtcgatcac catcagttcg cctggcccga cgcggccttt
     5401 ttcgaccact tcgtcaggct ggtaatccca gataccgact tcagaggcgc aggtgatgag
     5461 cttgtctttg gtaatgacgt agcgtgccgg acgcagaccg ttacggtcga ggttacaggc
     5521 agcgaagcga ccatcggaca tcacgatgcc cgccggacca tcccacggct ccatatgcat
     5581 ggagttaaag tcaaagaagg cacgcagttc cgggtccata tccgggttgt tctgccaggc
     5641 gggtggcact aacaggcgca tggcgcggat gatatccatc ccgcctgcca gcagcagttc
     5701 cagcatgtta tccatggaac tggagtcaga accggtttcg ttgacgaatg gcgcggcgtc
     5761 gtgcaggtca gggatcagcg gcgtctggaa tttataggta cgcgcacgcg cccattggcg
     5821 gttaccggtg atggtgttga tttcaccgtt atgcgccaga tagcggaacg gttgcgccag
     5881 cggccagcgc ggtacggtgt tagtggagaa gcgctggtgg aacaggcaaa tggccgattc
     5941 cagacgcagg tccgcaagat ccagataaaa gcgcggcaga tccgccggca tacacagacc
     6001 tttatagatg ttcaccagat tcgacaggct acagacgtag aagtctttgt cggcttcgag
     6061 acgcttttca atgcggcggc gggcgataaa cagacggcgc tccatatcgc gtggacgcca
     6121 gcctgccggg gcgttcacaa aaatttgctc aatgcgtggc agagaggaga gggcgatttc
     6181 acccagcacg ccttcgttag tggggacatc acgccagccc acaatcgaca aggtttcgcg
     6241 ttgcagttct tcttcaacga tgcggcgtgc ggcagcggcg agttcaggat ctttattcag
     6301 gaagagcatc ccgacagcgt agttttttgc taaacgccag ccgcgctcct gcgcaacgat
     6361 gcgaaaaaag cgatccggtt tttgtaacag caagccgcaa ccgtcgccgg ttttaccatc
     6421 ggcgagaatc gcgccacggt gctgcatgcg ggccagtgcg tgtattgcag tacgcactac
     6481 cttgtggcta ggttcgcctt ctatgtgggc gatcaggccg aaaccacagt tatccctctc
     6541 aagggattta tcgtacaaca tatcgtgaac ctcccccagg ctctgcggga accccacttc
     6601 cgaaccgctg cgcacgggca cagaaagagc gtggcgacgg ggtttgcgtg tcatacgcgc
     6661 gcctcgcatt cgcccccttg ttgtcctttt cgcatcggtt aatacggtga agaggggatc
     6721 tcaattactg cataaatatg atgagcaggc tgctcatcca gaaagcttcc agcggatttc
     6781 caacttatcg ggaaagctga tacaggtcaa atggcaagct tattggtaca gaaatgtgct
     6841 ttagcgcaaa attctttatt aaatagctga aattaaaata gattttatct ttaaggctaa
     6901 aaggagagtc gttagagaaa cagtcaattc ctgactgtca taaaggcgtg ttaaccctta
     6961 tcctgccttg aactgacgag aaaaccagca ttttatactg ccttaattgg taatgacgtt
     7021 tcatgctact gtttttgcct aaaatccata aggagataaa aaaatctgac acaagataag
     7081 caaaagtgat ttatgtgatt gtgcatgaaa tttcggtaat tttgattgat tatgcagttg
     7141 ataaaagcag gccgggacga ttgatccagg tcatcgccga attgagctaa aaatggcagg
     7201 ctttggcctc ttttcaaggc gcggtctatc agattatgca gttacagaaa ttagtcaata
     7261 tgtttggtgg tgatctcacc cgtcgttatg ggcaaaaggt gcataagctg acgctgcacg
     7321 gcggttttag ctgccctaac cgtgacggta ccatcgggcg tggtggctgc acattctgta
     7381 atgttgcctc gtttgccgat gaagcgcagc agcatcgttc cattgccgag caactggcgc
     7441 accaggcgaa tttagttaac cgcgctaaac gctatctggc ctactttcag gcgtatacca
     7501 gcacctttgc ggaagttcag gtgctgcgtt ctatgtatca gcaggcggtg agccaggcca
     7561 atattgtcgg tttgtgtgtt ggtacccgcc cggactgcgt gccggatgcg gtgctggatc
     7621 tgctttgcga atataaggac cagggttacg aagtgtggct ggagctgggg ctacaaaccg
     7681 cccacgacaa aacactgcat cgcatcaacc gcggccatga ttttgcctgt tatcagcgta
     7741 caacccagct ggcacgtcag cgcgggctga aggtgtgttc ccacttaatt gtcggcctgc
     7801 ctggcgaagg gcaggctgaa tgtttgcaaa cgctggagcg agtggttgaa accagcgtgg
     7861 acggcataaa gctgcatccg ctgcatattg tgaaaggcag cattatggcg aaagcctggg
     7921 aagcggggcg tttgaacggt attgaactgg aggattatac gctcactgcc ggagaaatga
     7981 ttcgccatac gccaccggaa gtgatctacc accgcatttc cgccagcgcc cgtcgcccga
     8041 cgctgcttgc tccgctgtgg tgcgaaaatc gatggacggg aatggtcgag ctggatcgct
     8101 atctgaatga acatggcgta cagggatcgg cgctgggacg tccgtggcta cctccaacgg
     8161 agtaggtcgt tgaggggaat tccgcatttc tcacacaatt tataacgtaa ctgtcagaat
     8221 tgggtattat tggggcaggt tgtcgtgaag gaattcccta atgaagcaaa ttcgtctgct
     8281 ggcgcagtat tatgttgacc tgatgatgaa gttaggtctg gtgcgcttct caatgttgct
     8341 ggcgctggcc ctcgtcgttc ttgccattgt ggtacaaatg gcggtaacca tggtgctgca
     8401 tggtcaggtc gaaagcattg atgttattcg ttctatcttc tttggtttgc tgattacgcc
     8461 gtgggcggtc tactttctat cggtggtcgt cgagcaactg gaggagtcac gacaacgtct
     8521 gtcacggctg gtgcaaaaac tggaggagat gcgcgagcgc gatttgagcc tcaacgttca
     8581 gttaaaagat aatattgccc agctaaatca ggaaattgcc gttcgtgaaa aagcggaagc
     8641 agaactgcag gaaaccttcg gccaactgaa aattgaaatc aaagagcgcg aagagacaca
     8701 aattcagctc gagcagcaat cctcattctt acgttccttc cttgatgctt cacccgactt
     8761 ggttttttat cgtaacgaag ataaagagtt ttccggctgt aaccgcgcga tggagctgct
     8821 gaccggaaaa agcgaaaaac aactggttca cctgaaacct gctgatgttt actcaccgga
     8881 agccgccgca aaagtcattg aaaccgatga aaaagtgttc cgtcataatg tgtcactgac
     8941 ctatgaacag tggctggatt acccggacgg gcgcaaagcc tgctttgaaa tccgtaaagt
     9001 gccgtactac gaccgcgtgg gtaaacgtca cggtttgatg ggctttggtc gcgacattac
     9061 cgagcgtaag cggtatcagg atgcgcttga acgcgccagc cgcgacaaaa cgacgtttat
     9121 ttccaccatc agtcacgaat tgcgtacacc gctgaacggt atcgtcggcc tgagccgcat
     9181 tctgctggat accgaactca ccgccgagca ggaaaaatat ctcaaaacta tccatgtttc
     9241 ggccgtcacg ctggggaata tctttaacga tattatcgac atggataaga tggaacggcg
     9301 caaggtccag cttgataatc agccggttga tttcaccagc ttccttgccg atctggaaaa
     9361 tctctccgca ttgcaggcgc aacaaaaagg attgcgcttt aacctggagc ctacgctgcc
     9421 attaccgcat caggtcatta ccgacgggac gcgtttacgg cagatcctgt ggaacctcat
     9481 cagtaacgcc gtcaaattca cccagcaagg ccaggttacc gtgcgcgtgc gctacgatga
     9541 aggcgatatg ctgcattttg aagtggaaga ttctggtatc ggcattccgc aggatgagct
     9601 ggataaaatc ttcgccatgt attaccaggt gaaagacagt catggcggta aacctgccac
     9661 cggcaccggt attggtctgg ccgtttctcg tcgtctggcg aaaaatatgg gcggcgatat
     9721 tacggttacc agtgaacagg gcaaaggttc aacctttacg ttgacgatcc acgcaccgtc
     9781 ggtggcagaa gaggtcgatg atgcgtttga tgaagacgat atgcctttac cggcgctgaa
     9841 tgtgctgctg gtggaagaca ttgaactgaa cgtgattgtc gcgcgttctg tgctggaaaa
     9901 attaggtaac agcgttgatg tcgccatgac cggcaaggcg gcgctggaga tgtttaaacc
     9961 gggcgaatac gacctggtgt tgctggatat tcagttgcca gatatgaccg ggctggatat
    10021 ctctcgtgaa ctgacgaagc gttatccgcg cgaggattta ccgccgctgg tggccttaac
    10081 cgctaacgtg ctgaaagaca aacaagagta cctcaatgct ggaatggatg atgtgctgag
    10141 taagccgctt tcggttccgg cgctaaccgc gatgatcaag aaattctggg atacccagga
    10201 tgatgaggag agtacggtga cgacagaaga gaacagtaaa tcagaagcat tgctcgatat
    10261 tcccatgctg gaacagtatc tcgaacttgt aggaccgaag ctgatcaccg acgggttagc
    10321 ggtgtttgag aagatgatgc cgggctatgt cagcgtgctg gagtcgaatc tgacggcgca
    10381 ggataaaaaa ggcattgttg aggaaggaca taaaattaaa ggtgcggcgg ggtcagtggg
    10441 gttacgccat ctgcaacagc tgggtcagca aattcagtct cctgaccttc ccgcctggga
    10501 agataacgtc ggtgaatgga ttgaagagat gaaagaagag tggcgtcacg acgtagaagt
    10561 gctgaaagcg tgggtggcaa aagccactaa aaaatgaccc cggctagacc ggggtgcgcg
    10621 aatactgcgc caacaccagg gaaatcttgg ctgcgccgta aattattatg atgagttaca
    10681 agggcacagc actgtttttc aggccgcacg cagttaagat agcaaaactt aaatgatttg
    10741 ttacatgaat cagttaaatg tgtgatgcgt accattttta ttaaaaaaat taatgtgttt
    10801 cagtcaattg cgaaaggatg atcacaatga agaaaattgg cgtaattctg agcggatgcg
    10861 gcgtctatga cggttctgaa attcatgaag cggtgttgac gttgttagct atttcacgca
    10921 gcggtgcgca ggcggtctgc tttgcaccgg ataagcagca ggttgatgtt atcaaccatt
    10981 taactggcga agcgatgacg gaaacgcgca atgtgctgat tgaagcggca cgaataacgc
    11041 gcggtgaaat ccgtcctctg gcccaggccg atgccgctga actggatgcg ttgattgtgc
    11101 cgggggggtt tggcgcggcg aagaatttaa gcaattttgc cagtcttggt agcgaatgca
    11161 ccgttgaccg tgaattaaag gcgctggcac aagcgatgca tcaggccgga aaaccgcttg
    11221 gttttatgtg tattgccccg gcgatgctgc cgaaaatttt cgatttcccg ctgcgtttga
    11281 ccatcggtac tgatatcgat accgcagaag tgctggaaga gatgggcgcg gagcatgtgc
    11341 cgtgtcctgt cgatgatatc gtggttgatg aagacaataa gattgtcacc accccagcat
    11401 atatgctggc gcagaacatt gcagaagcgg cgagcggcat tgataagctg gtttcccgcg
    11461 tgctggttct ggctgaatga gtaaaagccg cttaacggtg tttagtttcg ttcgccgttt
    11521 tcttttgcgg ttaatggttg tcctcgccgt tttctggggc gggggcatcg cgttgtttag
    11581 cgttgcgcct gttcccttct cagcggtaat ggtcgagcga caggtcagcg cctggctgca
    11641 tggcaatttt cgttacgtgg cacattctga ctgggtcagt atggatcaaa tctcgccgtg
    11701 gatgggactg gcggtgattg ccgcagaaga tcagaaattt cctgagcact ggggctttga
    11761 tgtcgcttcc attgagaaag ccctggcgca caacgagcgc aatgaaaacc gtattcgcgg
    11821 tgcttcaacg atttctcagc agacggccaa aatctctttt tatgggatgg gcgtagctgg
    11881 gtacgaaaag ggctggaagc tggattaacg ctggggatag aaacagtctg gagcaaaaag
    11941 cgtatcctga cggtttacct gaatatcgcc gaatttggcg acggtgtgtt tggcgtcgaa
    12001 gctgcggcac aacgttattt ccacaaaccc gcgagcaaac ttacccggtc ggaagctgca
    12061 ttactggcag ctgtattacc taatccactt cgtttcaaag tctcctcgcc atcgggctac
    12121 gtgcgtagcc gtcaggcgtg gattttacgg cagatgtatc agttaggcgg tgagccgttt
    12181 atgcaacaac accagctgga ttaagcttcg cgcgaagtga gggccagcgt tttgctctca
    12241 cgaggttgca gatctttctg cagggcaata gccagcgccg gttgggcgat aaatcgtttc
    12301 cataaccgct cttgcttacg cagacaacac cacttcgacc aggtcgccag tggaatcaat
    12361 gtgctttcac caatgttgtc gcagacgacc gggataacct ctgactcact gatgcggtga
    12421 cagaggatat tctgcggctt cagcgacatc gtcacgatac ggttatcctg caaataacgc
    12481 ttcagctgtt tcagtagctg gcgcagttgt gcaatgtctt cttcgtaacg gcattgttca
    12541 gcaaattcgg tcagcgtaat ggaaggcttg ccgtcaaaat cggcaatcac atcgtagaca
    12601 taaccggttc cgcaatccgt ctcaacggta ccgtgatagc gcggtattcc actccagtct
    12661 tttaaccggc gaccaagatg cgcgtagtac tttaactcac ggcggatctc tttatcgcca
    12721 ccatcgccac gatggtagac aatcttgata cagcgttggg catcttccgg atgcgcataa
    12781 catttccgat gtcttccggt gcccagggga ctttgttcag ataagcgaat catgccatct
    12841 cctggaaata ttttctgacg atctccagta tggtcgatta acttaaacaa aacctaaaca
    12901 atgcctctta aggttttctt aaggttcttc tgaaagtgaa aagcgcacaa aatgtacaaa
    12961 tgacgttgat tgagaacgat ttcagtacgt gattttctta ttgtcggggg agcttgaagg
    13021 gagttgtatg tcaaagtgat gaagattaat cttcatcaaa accagaatta aagagggcga
    13081 taacggcggc cagtgcttcc tcttcctgtg gaccggtcgc ttcaacttca atctgccgtc
    13141 ctttggcaga atccaacatc agcagcgcaa taacgctgtt ggcttcagcc tcggtgcctt
    13201 cgtcattacg taacagcact tcagcgtcaa aaccctgcat taattcaaac agcttcattg
    13261 caggccgggc atgcatgccc agcttgtttg tgatttcaac agtttgcttg acggtcatgg
    13321 tttacgtttt tccagcgtac gatggcgtga ctggacgttt ttaccgcgcg agcggaagta
    13381 gtctgccagt tgctctgcaa tatacaccga acggtgcttc ccgccggtac aaccaatggc
    13441 gaccgtcagg tagctacggt tgttggtttc cagcataggt aaccatagct caagatagct
    13501 tcgcgtctgg tagataaaat tgtgtacttc tgtgtggcgg tcgaggaacg cggcgacagg
    13561 tttatcaaga cctgtcattg gacgcagttt cggatcccag tgcgggttcg gcaagaagcg
    13621 cacgtcaaag acgtaatctg catcgatagg gataccgtgt ttgaagccga aagactcaaa
    13681 gaccatggtc agttcgcgtt cacgtttacc cagcagacgg gtacgcagca tttcagccag
    13741 ctcgtgaacg gacatttctg aggtatcgac aatcagatcc gctcgcgaac gcagaggctc
    13801 cagcaaatcg ctttctttgt cgatagcact ttccagcgac aggtttttgc tggaaagcgg
    13861 atgcagtcgg cgcgtgtcac tgtaacgacg aattaaggta ttacggtcgg catccaggaa
    13921 cagtagttgc ggtgagaaag cgtcaggcag gttactcatc gcctgttcga atatttctgg
    13981 tgactccggc atattacgaa catcaatgct gacggcggca gaaatctcgc gatcggccag
    14041 agttcgagcc agatcgggta acaacactac gggaaggtta tccacgcagt aaaaacccat
    14101 atcttccagc gcacgcaggg cgacagattt acctgaacct gaacgtccgc tgacgatcat
    14161 cagtaccatg tactgtttct cctcacaacg tctaaaagag acattaccga ataactacgc
    14221 ttcatccgga gtaccttcgg tatccgtaat gatttgatac agctcttcat cgctctgggc
    14281 tgcgcgtaaa cggcggcaga tggttttgtc cgccagacgt ttcgccacca gcgacagagt
    14341 atgcaggtgc gttttagttt ggtctgccgg caccagcagg gcaaaaagta agtccaccgg
    14401 ttggttgtcg atggcatcga aagctatagg cgtttcgagc tgaacgaaaa cgccaacggc
    14461 acgcagagta tcttcttcaa gtttgccatg cggaatggca ataccattgc cgataccggt
    14521 actgcccatt ttttcccgcg tcaggatagc ttcaaaaacc acctgaggcg gcaggctaag
    14581 ttgtttcgcc gccagctcgc tgatgatttc cagggcgcgt tttttgctct gacagtggac
    14641 gcggcttcgc gtacattccc tgttaagaac actgctaagc tgtagagttg tatcattatt
    14701 tgtcataatt tcacctaaga acctgcccac tcaaacagtg gaacaggctc ggagcattaa
    14761 ccgtacaaat ggcccgttgt gccgcacaac aggccgccat gcacatgcta attgcccgga
    14821 caattagtgt tgtttcagtt tatctttatg tttggtcaac tggcgtgcca gcttatcaat
    14881 taagccatca atggcagcgt acatgtcctg accttccgcg ctggcatgaa tttcaccacc
    14941 gtttacatgc agtgttgcat ctgaggtgtg ggtgactttc tccactttca gaacaacata
    15001 gacctggttg attcggtcaa aatattgctc aagtttggca aatttagctg taacaaattc
    15061 gcgcagtgcc tcggtgatct cgacgttatt tccggtaatg ttgagctgca tagtgtcttc
    15121 cttatcggtt gggtcaaacg agctgtttac gctggtttga cggcggaatg gataaagact
    15181 ctcggtactt cgcaacagtg cggcgtgcca ccatgatacc ttgttccgac agcaaagagg
    15241 ttaacttgct gtcgctcaac ggtttcgctg ggttttccgc cgcgattaat ttcttcacca
    15301 gtgcacgaat cgccgtggag gaagcttcgc cgccgccctc ggtattgacg tgactggaaa
    15361 agaaatactt cagttcaaaa atgcctcgtg gactatgcag gtatttttgc gtggtcacgc
    15421 gagatatcgt cgattcatgc atttcgacag cctgggcgat atcggccagt accatcggtt
    15481 tcatatattc ttcaccttgc tcaaagaagg cttgctgctg ttcaacgata cagcgactca
    15541 cgcgcagtag cgtatcgtta cggctttcca gactcttgat caaccatttg gcatcctgca
    15601 gattgctgcg gataaactgg ctgtcaccat cgttgcgcgc gttattgcac atcgaggcgt
    15661 agtgctggtt gatttgcaga cgcggaatgc tgtcactgtt gagttctacc gtccagtgac
    15721 cgttatgctt acgcaccagc acatctggaa tgacatactc aggttcgcca gtctggatcg
    15781 actgcccggg gcgcggatcg agcgactgga tcagattgac ggcttctttc agcacatctt
    15841 ctttcagacg cgtgacgcgc attaaagtgc ggaagtcgtg attggctaac agatcgagat
    15901 gatcgctaat gatcaatctg gcctcttcca gccacggcgt ggtcttatcg aattgggaga
    15961 gttggattag caggcagtca cgcagatctt ttgccgctac accgaccggg tcaaaccgtt
    16021 ggatccgctt aaggacggct tcaacctcgt cgatgtcgat ctcttcatcg cccatacttt
    16081 cgagaatatc ttccagcggg acagtcagat aaccggtttc atcaacggca tcgacgatag
    16141 aggtagcaat agcgcggtca gtgtcggaaa acggtgtcag ctcgacctgc cacatcaggt
    16201 aatcctgcaa ggtctgcgtc gtttcgccct ggtagaccgg cagctcgtcg tcaatgtagt
    16261 caccgctggt gccggatggt gtaccagcgg tgtaaatggt gtcccaactg gcatcgagcg
    16321 gcagctcttc cggcatctct ttttgttcga gcgcgtcggc ggtgtccagc gtttcactgt
    16381 cttgcgtttc gcgggtgtcg atttcttcat gagtgtcgat ttgctcaagc agcggattac
    16441 tctccagcgc ctgctgtagc tcctgctgaa gttccagcgt cgacaactgc aacagacgaa
    16501 ttgcctgttg gagctgtggc gtcatcgcca gttgttggct aagcctgagt tgcaaacctt
    16561 gcttcatgtt cagaatcgta ctctcctgct aaaacgtcgc aaacttctac cctatcagag
    16621 tctgaagtct tccccaaggt atacacgctt aacgtgttcg tcttgtaaga tttctgtagg
    16681 cgtgccgtgg gcgatcaaat gcccctgact gacgatataa gcgcgttcac aaaccgccag
    16741 tgtttcacgc acgttgtggt cagtgatcag cacgcccagg ccgctgtcgc gcaggtgttc
    16801 aatgatgcgt ttaatgtcga taaccgagat cgggtcaacc ccggcaaacg gttcgtcgag
    16861 cagaataaat ttcggattcg cagccagtgc gcgggcaatt tctacacggc gacgttcacc
    16921 cccggagagt gactgcccca tgctgtcacg caggtgctca atgtgaaact cttccatcag
    16981 ctcgttcgcg cggtcttcac gttgttcagc agacaagtcg tcacgaattt gcagtaccgc
    17041 catcaggtta tcgtaaacgc tgaggcgacg gaaaatggag gcttcctgtg gcagatagcc
    17101 gataccgcgg cgcgcgcgtg catgcagagg cagcagactg atatcgtcat catcaataat
    17161 gatgttgccc gcatcgcgcg gcacaatgcc tacaaccatg tagaaagtgg tggtcttacc
    17221 ggcaccgttt ggccccagca gaccgacaat ttccccggag ttgacggtca ggctgacgtc
    17281 ttctaccacg cgacggcctt tataggcttt tgcaaggttc tttgcagtta atgttgccat
    17341 aacgaattaa ttacccttct tctgtgccgg ggtctggcct ttgttgtttt tgtcctgcag
    17401 ctgcgacggc accagaacgg tggttacgcg cttgcctttg tcgctgaaag cctgcatttt
    17461 ctgctctttc accaggtaag tgatcttatc gcccttaatg ttgctatcga cctgctgcag
    17521 ataagcatta cccgtcagaa cgacaaaatc ttttgccagt tcgtagtgca tctgggaagc
    17581 gtgaccttca acgggtttac cgttgtcctg catctggtag aacgttgccg gtttaccgta
    17641 tccgtcaatc acttctttac cttgttcgcc gcccggacgg gtaacgacca ctttgtcggc
    17701 attaattttg atggtgccct gggtgacgat gacattaccg gtaaaggtaa ccacgttgcc
    17761 ttgcatatca agagattgct ggtccgattc aatgtggatc ggctgatcag tgtctccggt
    17821 tacggcaaat gccggaatgc tggcggccag aagtgagctg gcaagcacaa gattaaggct
    17881 gagtttgttt gttttgaatt tcataggatg ttctaacctt ttcaatcagc tcggcgttct
    17941 tgctgcgtaa gttgccgcgc attttcagac cgctggagtt aaatgttgtt ccgtataacg
    18001 tgacgaggtc ttcagaggta acatcctgcg tcaccagatt gatctgcgcg ttatccgtcg
    18061 tgattctgcg aagttgagag tctggcacaa gtgcgttgac ttcaacgtgt ccatataaat
    18121 agagcatccg gtcattggtc agcttggctt tatctgcttt tacggaccat gtcgggattt
    18181 tatccttatc aaacgtggta agtaccggct gcgtaaacca cgaaacggcc tgatcggaat
    18241 aatattcaac gtgttgagca atcaatcgat agcttagtgc cccttctggg ttatagacga
    18301 gcgtgtccgt atgctcgctt ttataggtgg gatcattgtt gttgacgacc acctgggcgg
    18361 tatcgtcttt ttcggccata ttaatgccga tcatcaccag aaccgccagt gatagcacaa
    18421 tgataaccca acgtctggct ttactcatat cgattgccct ttggcttcat ccagtttgcc
    18481 ctgcgccagg agtaataagt cgcaaacttc gcgcactgcg ccacgaccgc cagcaatgcg
    18541 cgtcacgtaa tcggcgcgcg ggatcaacag tggatgcgca tcggccacgg cgacgcttaa
    18601 acccactttt tccattaccg gccagtcgat gagatcatcg ccaacataag ccacattttc
    18661 cggggcaatc gccaattttt ccagcaaatc gctaaaggcg atcagtttgt ttgactgccc
    18721 ctgatacaag tgagtgatcc ccaatgtggc acaacgatct tctaccagtt tagcctttcg
    18781 cccggtaatg atagcgactt caatatcaga ggtgagcgca caacgaatgc cataaccgtc
    18841 acgaacattg aacgctttca gctcttcgcc gttattgccc atataaatca ggccatctga
    18901 cagtacgcca tcaacgtcga ggatcagcag acgaatgttc tctgcttttg ccataacgtc
    18961 ggcgctgaca gggccgtaac aggtcgcaag cgacgcacct gctttgctca ttgttgttta
    19021 tccttgaatc tttacactac gcctgcacgc agtaaatcat gcatatgtaa cacaccgagt
    19081 aaatggtcgc catcggcaac catcacggag gtgatatggc gggactgcat taagttcagt
    19141 gcctcaacgg ccagaatgcc agggcgcaca cgtattcccc ccggcgtcat cacatcggca
    19201 atacttaact gacgaacatc cacgcccata tcgaagacac ggcgtaaatc accgtcggta
    19261 aagatgcctt caatcatcat attgtcatcg caaatgacag tcataccaag atttttgcgg
    19321 gtaacttcca gcaatgcgtc acgcagactg gccgttttct taacatgcgg gatctcatcg
    19381 cccgtatgca taatatcgtt tacgcgcagc agaagtttac gacccagtgc gccgcctggg
    19441 tgtgagagcg caaaatcttc agcagtaaag ccgcgtgctt ttaacagcgc gacagcgagg
    19501 gcatcgccca taaccagcgt ggcggtggtg ctgctggtcg gtgccagccc taacggacag
    19561 gcttctttcg ctactttaac acacagatgc acatctgcgg cgcgcgccat gctgctctcc
    19621 gggcgaccgg tgatgcagat taacggtacg tgaagacgct taagcactgg aattaaggcc
    19681 gtgatttcgc tggattcacc tgagttagag atagcaatca ccacatcctg tggggtgacc
    19741 atgcctaaat caccatgcgc ggcttcacta ggatggacga aaaatgaagg tgtaccggtg
    19801 ctggcaaacg ttgcggccat tttgcgcccg atgtgccccg atttccccat ccccatgacg
    19861 acaactttcc ctttacacca gaacatcttt tcacaggcaa gcgtgaaatt ctgattgatg
    19921 tattgatcaa gctccgccag gcattcacgt tcaatcgcca ggacttcttt acctgcttgc
    19981 tgaaagtcaa aacccggttg taactctacg tgcgacataa tgcgtttcca gttattcaac
    20041 gagtattggc gataaccagt acaacatcgc cagccatacg ataaatccgc cagttaataa
    20101 tacccctaca ccacggcccg gttgcgggga gcgccgccag cacagcaacg caaaaataat
    20161 gctcaccagc aacatcacgc tgtagtcacg actgtacgcc agtggatcaa tctctcctgg
    20221 cgttatcagc gcgggtaaac ccaacacgat gacaatatta aaaatgtttg cgccaatgat
    20281 atttccgaca gcaatgtcgt tttcaccttt gcgaaccccc gctattgcgg ttgccagttc
    20341 cggcagactg gttccgatag caattgccgt cagagccatc gtcaactcgc tgatggcaaa
    20401 gtaattcgcc agcaccgtgg cgttatcaac caccatccgc gtggccactg gcatgatgat
    20461 aagcgcaatg ccgagccata aaaacgcgac gggcaatccg ccgtcacgcg gcagctctgc
    20521 aagctgctct ctggtcaggc tgtcagtccc ctgacgtgca agtttaacaa tgaacagcag
    20581 ccatagcaca gccagaaaga ggagaaagat accatcgctt cgactaagtt gtccgtcata
    20641 gagtacggaa ccggccacca cgctgaccaa caacattaag ggtaattcac ggcgcagaac
    20701 atcggaatgg acggtaaaag gacgaaccag cgcagccaga ccgaggatca gcaatatatt
    20761 gataatgttt gagccgaggg ctgtaccaac ggctaaatcg cgttgttcgt gcagagacgc
    20821 ggcaagcgag acgatgattt ctggtaacga tgtaccaata ctgaccaccg tcatgccgat
    20881 gatcagcggc gggatgccaa aggttcggca aagaatagac gcggcaaaaa ccaggcggtc
    20941 ggcactgtaa acgaccaaaa gtaaaccaac aattaacagt gccgtagcta aaagcatcta
    21001 acgtcctttc ttcaggtata ctcgccggtc cgctgaagat tttcagaaag ccgtaacgga
    21061 ttcttaattt tgactttatg cggctaaaaa gtaaaacaaa tgccagcttt cgctaaccac
    21121 ggcgggtaat attctgtaaa tatgttgggt tcaaggttaa attgagcgcc atgcttagaa
    21181 aatcaacgca agacgaaggg tgaattatgg agcagtctgt ggcgaattta gtcgatatgc
    21241 gcgatgtcag ttttacgcgt ggcaatcgct gcatcttcga taatatttcc ctgaccgtgc
    21301 cgcgagggaa gatcacggcg atcatggggc catcgggcat cggtaaaacg acgctactcc
    21361 gtctgattgg cgggcaaatc gcaccagatc atggtgagat ccttttcgat ggtgagaata
    21421 ttccggcgat gtctcgttcg cgcctgtata cagtgcgtaa acggatgagc atgttatttc
    21481 agtccggggc gttgttcact gatatgaacg tatttgacaa cgtcgcctat ccactgcgcg
    21541 aacataccca acttcccgcg ccattgttgc atagtacggt gatgatgaag ctggaggccg
    21601 tggggctgcg tggagcggct aaactaatgc cttctgaact ttccggtggg atggcgcggc
    21661 gtgcagcgct ggcgcgtgcg attgcgctgg agccggatct catcatgttt gatgaacctt
    21721 ttgttgggca agatcccatc accatgggcg tactggtgaa gctgatttct gagctgaaca
    21781 gcgcgctggg cgtgacttgt gtggtggttt ctcacgatgt gccggaagtg ttaagtattg
    21841 cggatcacgc ctggatcctg gcggacaaaa aaattgtcgc tcatggcagt gcccaggcgt
    21901 tgcagacgaa tcctgatccg cgcgtacgtc agtttctgga cgggatagct gacgggcctg
    21961 ttccgttccg ctatcctgcc ggcgattatc acgctgatct tttaccaggg agttaagcca
    22021 ctcatgctgt taaatgcgct ggcgtcgctc ggacataaag ggattaaaac cctgagaacg
    22081 ttcgggcggg ccgggttaat gttattcaat gcgctggtcg gcaaaccgga atttcgcaaa
    22141 catgcgccgc tgctggtgcg ccagctctat aatgtcggcg tcctgtcgat gctgattatt
    22201 gtagtttctg gcgtgttcat cggaatggtg ttggggctgc aaggttatct ggttctgacc
    22261 acttatagcg cggaaaccag tctgggtatg ctggtggcgt tatcgctact gcgtgaactg
    22321 gggccggtgg ttgccgcgct gttgtttgcc gggcgtgctg gttcggcgtt aaccgcagaa
    22381 atcggcctga tgcgcgctac agagcaactc tccagtatgg agatgatggc ggtggatccg
    22441 ctgcgtcggg ttatttctcc ccgtttctgg gctggggtta tttcattacc actgttgacg
    22501 gttatcttcg tcgccgtggg gatctggggc ggatcgctgg tcggcgtcag ttggaaaggc
    22561 attgatagcg ggttcttctg gtcggcaatg caaaatgccg tcgactggcg tatggatctg
    22621 gtcaactgtc tgattaagag cgtggtgttc gccatcacgg tgacgtggat ttcgttgttt
    22681 aacggctacg acgccatccc gacgtctgcc gggattagcc gggcaaccac tcgcaccgtt
    22741 gtccactcgt ctctggctgt tctggggctg gattttgtgc tgaccgcatt gatgtttggg
    22801 aattgagttc atgcaaacga aaaaaaatga aatttgggtg ggtatctttt tattagcagc
    22861 actgctggcg gcgctgtttg tttgcctgaa ggcggcgaac gtgacgtcca tacgtactga
    22921 atcgacctac acgctttatg cgacgttcga taacattggc ggcctgaaag cgcgctctcc
    22981 ggtcagcatt ggtggtgttg ttgtgggccg ggtggcggat attacgctgg acccgaaaac
    23041 ctatctgccg cgcgtaacgc tggaaattga acaacgttat aaccacattc ctgataccag
    23101 ttcgctgagc attcgtactt ccggcctgct gggagaacaa tatctggcat taaacgtcgg
    23161 ttttgaagac ccggaactgg ggactgctat cctgaaggat ggcgatacaa ttcaggacac
    23221 caagtctgcg atggtgctgg aagatctcat tggtcagttc ctttacggta gtaaaggcga
    23281 tgacaataag aatagtggcg atgcgccagc tgctgcgcca ggtaataatg aaaccactga
    23341 acctgtgggt acaacgaaat aatttcagga gaaccgacgc atgtttaaac gtttaatgat
    23401 ggtcgctttg ctggtgattg cacctctgag tgcggcaacc gcggcagacc agaccaatcc
    23461 ttataagctg atggacgagg cggcgcagaa aacgttcgat cgcctgaaga atgagcaacc
    23521 gcaaattcgg gccaacccgg attatctgcg taccattgtt gatcaggaac tgctgccata
    23581 cgtacaggtg aaatacgccg gtgcgctggt gctgggccag tattacaaga gtgcgacccc
    23641 tgctcaacgt gaagcctact ttgccgcttt ccgtgagtac ctgaagcagg cttacggtca
    23701 ggcgctggcg atgtatcacg gtcaaaccta tcagattgcg ccagaacagc cgctgggcga
    23761 taaaaccatt gtgcctattc gcgttaccat tattgacccg aatggccgtc cgccggtgcg
    23821 tctggacttc cagtggcgta aaaactccca gacgggaaac tggcaagctt acgacatgat
    23881 tgctgaaggc gtcagtatga tcaccaccaa acaaaacgag tggggaacgc tgctgcgtac
    23941 caaaggtatc gacggcctga ctgcgcaact gaaatcgatt tctcaacaga aaatcactct
    24001 ggaagagaaa aaataatgag cgagtcactg agctggatgc agacgggtga cacgctggcg
    24061 ttatccggag agctggatca ggacgttctg ctaccgcttt gggaaatgcg tgaggaggcg
    24121 gtgaagggga ttacctgcat cgatcttagc cgtgtgtccc gcgtggatac ggggggactg
    24181 gcactgctgc tccatcttat cgatctggcg aaaaagcagg gcaacaacgt gacgcttcag
    24241 ggggtaaacg acaaagtgta taccctcgcg aaattgtata atttgcctgc tgatgttctg
    24301 cctcgttaaa ttttttcagg ccggatatgg cgttaaccgc ctgtccggca aacctgcctg
    24361 caagtcgagc cttgtctgat ttatcagcga ggctttttgc ttatttatgc cagcgcaact
    24421 ttgctctaag atgtttcgct ggtttattaa ctgatgattg aagatcccat ggaaaataat
    24481 gaaattcaga gcgtgttgat gaacgctctc tccctccagg aagtccacgt ttccggcgat
    24541 ggcagccact ttcaggttat tgccgtgggt gagttgtttg acggtatgag tcgggttaaa
    24601 aaacagcaga cggtctatgg cccgctgatg gaatatattg cggataaccg cattcatgct
    24661 gtgtcgatca aagcgtatac ccctgcggag tgggcgcgcg atcgcaaact gaacggcttt
    24721 tgagctatgg gcgattcgcc ggtagcggat atgaattgtt aactgagaac aaactaaatg
    24781 gataaatttc gtgttcaggg gccaacgaag ctccagggcg aagtcacaat ttccggcgct
    24841 aaaaatgctg ctctgcctat tctttttgcc gcactactgg cggaagaacc ggtagagatc
    24901 cagaacgtcc cgaaactgaa agacgtcgat acatcaatga aactgctaag ccagctgggt
    24961 gcgaaagtag aacgtaatgg ttctgtgcat attgatgccc gcgacgttaa tgtattctgc
    25021 gcaccttacg atctggttaa aaccatgcgt gcttctatct gggcgctggg gccgctggta
    25081 gcgcgctttg gtcaggggca agtttcacta cctggcggtt gtacgatcgg tgcgcgtccg
    25141 gttgatctac acatttctgg cctcgaacaa ttaggcgcga ccatcaaact ggaagaaggt
    25201 tacgttaaag cttccgtcga tggtcgtctg aaaggcgcac atatcgtgat ggataaagtc
    25261 agcgttggcg caacggtgac catcatgtgt gctgcaaccc tggcggaagg caccacgatt
    25321 attgaaaacg cagcgcgtga accggaaatc gtcgataccg cgaacttcct gattacgctg
    25381 ggtgcgaaaa ttagcggtca gggcaccgat cgtatcgtca tcgaaggtgt ggaacgttta
    25441 ggcggcggtg tctatcgcgt gctgccggat cgtatcgaaa ccggtacttt cctggtggcg
    25501 gcggcgatct ctcgtggcaa aattatctgc cgtaacgcgc agccagatac tctggacgcc
    25561 gtgctggcga aactgcgtga tgctggagcg gacatcgaag tcggcgaaga ctggattagc
    25621 ctggatatgc atggcaaacg tccgaaggct gttaacgtac gtaccgcgcc gcatccggca
    25681 ttcccgaccg atatgcaggc ccagttcacg ctgttgaacc tggtggctga agggaccggg
    25741 ttcatcaccg aaacggtctt tgaaaaccgc tttatgcatg tgccagagct gagccgtatg
    25801 ggcgcgcacg ccgaaatcga aagcaatacc gttatttgtc atggtgttga aaaactttct
    25861 ggtgcacagg ttatggcaac cgatctgcgt gcatcagcaa gcctggtgct ggctggctgt
    25921 attgcggaag ggacgacggt ggttgatcgt atttatcaca tcgatcgtgg ctacgaacgc
    25981 attgaagaca aactgcgcgc tttaggtgca aatattgagc gtgtgaaagg cgagtaatcg
    26041 tctgagagct ggcagccccg ttcgcggggc taccgccaga ccactcattt tttcggttta
    26101 gtgtagctac gcatcaactg cgttctgtcg ataaactcat gggtctgcgg atcatggtag
    26161 cgtgatggcc agataaccca ggggtcagtt cccagggctt tcgcaataat catctctcct
    26221 ttcggccatg ggcgcgataa tgcgtttgcc agcgttgagg aactcaaacc atttctgcga
    26281 gattccgccg ccattgacgt tcctttcttg cgcaaacctg caatgatgtc agcgggatgc
    26341 cagtcaatga aattactttc catctttcct cactccttaa gttagtcgct ttcattcaga
    26401 acacttcttc tgtcgggcat cactatactc ctgatggtct attgcgctaa aaagttcctg
    26461 taaagagtgt gaatattcag aatatataaa gattcatcgc ccttttttca ggtgttatag
    26521 cgctgaatta gtgtttttat
//
LOCUS       DAZPEU010000037        26184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPEU010000037 DAZPEU010000000
VERSION     DAZPEU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.037"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..213)
                     /locus_tag="ACHU2L_001427"
     CDS             complement(<1..213)
                     /locus_tag="ACHU2L_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR1356353.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="ACHU2L_001428"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="ACHU2L_001428"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR1356354.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="ACHU2L_001429"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="ACHU2L_001429"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR1356355.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="ACHU2L_001430"
     CDS             2894..3634
                     /locus_tag="ACHU2L_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1356356.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHU2L_001431"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHU2L_001431"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR1356357.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="ACHU2L_001432"
     CDS             complement(4192..4794)
                     /locus_tag="ACHU2L_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR1356358.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="ACHU2L_001433"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="ACHU2L_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR1356359.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="ACHU2L_001434"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="ACHU2L_001434"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR1356360.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="ACHU2L_001435"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="ACHU2L_001435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR1356361.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="ACHU2L_001436"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="ACHU2L_001436"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR1356362.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="ACHU2L_001437"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="ACHU2L_001437"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR1356363.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="ACHU2L_001438"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="ACHU2L_001438"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR1356364.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="ACHU2L_001439"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="ACHU2L_001439"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR1356365.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="ACHU2L_001440"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="ACHU2L_001440"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1356366.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="ACHU2L_001441"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="ACHU2L_001441"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR1356367.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="ACHU2L_001442"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="ACHU2L_001442"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR1356368.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="ACHU2L_001443"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="ACHU2L_001443"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR1356369.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="ACHU2L_001444"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="ACHU2L_001444"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR1356370.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="ACHU2L_001445"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="ACHU2L_001445"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR1356371.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="ACHU2L_001446"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="ACHU2L_001446"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR1356372.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="ACHU2L_001447"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="ACHU2L_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /gene="yebF"
                     /locus_tag="ACHU2L_001448"
     CDS             20653..21009
                     /gene="yebF"
                     /locus_tag="ACHU2L_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR1356373.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="ACHU2L_001449"
     CDS             21336..21995
                     /locus_tag="ACHU2L_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR1356374.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="ACHU2L_001450"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="ACHU2L_001450"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR1356375.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="ACHU2L_001451"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="ACHU2L_001451"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR1356376.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /gene="yobB"
                     /locus_tag="ACHU2L_001452"
                     /pseudo
     CDS             complement(24948..25604)
                     /gene="yobB"
                     /locus_tag="ACHU2L_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="ACHU2L_001453"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="ACHU2L_001453"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR1356377.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..>26184
                     /locus_tag="ACHU2L_001454"
     CDS             26075..>26184
                     /locus_tag="ACHU2L_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356378.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAA"
BASE COUNT         6652 a         6485 c         6818 g         6229 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatgggtat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggc
//
LOCUS       DAZPEU010000038        25923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPEU010000038 DAZPEU010000000
VERSION     DAZPEU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.038"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHU2L_001455"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHU2L_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR1356379.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHU2L_001456"
     CDS             complement(1614..1937)
                     /locus_tag="ACHU2L_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR1356380.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHU2L_001457"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHU2L_001457"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR1356381.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHU2L_001458"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHU2L_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR1356382.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHU2L_001459"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHU2L_001459"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR1356383.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHU2L_001460"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHU2L_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR1356384.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHU2L_001461"
     CDS             7537..8574
                     /locus_tag="ACHU2L_001461"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1356385.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHU2L_001462"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHU2L_001462"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR1356386.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHU2L_001463"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHU2L_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR1356387.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHU2L_001464"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHU2L_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR1356388.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACHU2L_001465"
     CDS             10745..11626
                     /locus_tag="ACHU2L_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1356389.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHU2L_001466"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHU2L_001466"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR1356390.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACHU2L_001467"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACHU2L_001467"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR1356391.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHU2L_001468"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHU2L_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACHU2L_001469"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACHU2L_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR1356392.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHU2L_001470"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHU2L_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR1356393.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACHU2L_001471"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACHU2L_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR1356394.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACHU2L_001472"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACHU2L_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR1356395.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACHU2L_001473"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACHU2L_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR1356396.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACHU2L_001474"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACHU2L_001474"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR1356397.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACHU2L_001475"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACHU2L_001475"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR1356398.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACHU2L_001476"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACHU2L_001476"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR1356399.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACHU2L_001477"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACHU2L_001477"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR1356400.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACHU2L_001478"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACHU2L_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR1356401.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHU2L_001479"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHU2L_001479"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR1356402.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACHU2L_001480"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACHU2L_001480"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR1356403.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACHU2L_001481"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACHU2L_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR1356404.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         6369 a         6459 c         6841 g         6254 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagccactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 agg
//
LOCUS       DAZPEU010000039        25592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPEU010000039 DAZPEU010000000
VERSION     DAZPEU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.039"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHU2L_001482"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHU2L_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHU2L_001483"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHU2L_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR1356405.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFVSGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHU2L_001484"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHU2L_001484"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR1356406.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHU2L_001485"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHU2L_001485"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR1356407.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHU2L_001486"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHU2L_001486"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR1356408.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHU2L_001487"
     tRNA            7783..7858
                     /locus_tag="ACHU2L_001487"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
     gene            8016..8091
                     /locus_tag="ACHU2L_001488"
     tRNA            8016..8091
                     /locus_tag="ACHU2L_001488"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Gly,seq:gcc)
     gene            8127..8202
                     /locus_tag="ACHU2L_001489"
     tRNA            8127..8202
                     /locus_tag="ACHU2L_001489"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8160..8162,aa:Gly,seq:gcc)
     gene            8436..8489
                     /locus_tag="ACHU2L_001490"
     CDS             8436..8489
                     /locus_tag="ACHU2L_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356409.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8472..9611)
                     /gene="queG"
                     /locus_tag="ACHU2L_001491"
     CDS             complement(8472..9611)
                     /gene="queG"
                     /locus_tag="ACHU2L_001491"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR1356410.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9610..11157
                     /gene="nnr"
                     /locus_tag="ACHU2L_001492"
     CDS             9610..11157
                     /gene="nnr"
                     /locus_tag="ACHU2L_001492"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR1356411.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            11129..11590
                     /gene="tsaE"
                     /locus_tag="ACHU2L_001493"
     CDS             11129..11590
                     /gene="tsaE"
                     /locus_tag="ACHU2L_001493"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR1356412.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11609..12946
                     /gene="amiB"
                     /locus_tag="ACHU2L_001494"
     CDS             11609..12946
                     /gene="amiB"
                     /locus_tag="ACHU2L_001494"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR1356413.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            12956..14803
                     /gene="mutL"
                     /locus_tag="ACHU2L_001495"
     CDS             12956..14803
                     /gene="mutL"
                     /locus_tag="ACHU2L_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR1356414.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14796..15746
                     /gene="miaA"
                     /locus_tag="ACHU2L_001496"
     CDS             14796..15746
                     /gene="miaA"
                     /locus_tag="ACHU2L_001496"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR1356415.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15832..16140
                     /gene="hfq"
                     /locus_tag="ACHU2L_001497"
     CDS             15832..16140
                     /gene="hfq"
                     /locus_tag="ACHU2L_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR1356416.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16216..17496
                     /gene="hflX"
                     /locus_tag="ACHU2L_001498"
     CDS             16216..17496
                     /gene="hflX"
                     /locus_tag="ACHU2L_001498"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR1356417.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17582..18841
                     /gene="hflK"
                     /locus_tag="ACHU2L_001499"
     CDS             17582..18841
                     /gene="hflK"
                     /locus_tag="ACHU2L_001499"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR1356418.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18844..19848
                     /gene="hflC"
                     /locus_tag="ACHU2L_001500"
     CDS             18844..19848
                     /gene="hflC"
                     /locus_tag="ACHU2L_001500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR1356419.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            19930..20127
                     /locus_tag="ACHU2L_001501"
     CDS             19930..20127
                     /locus_tag="ACHU2L_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR1356420.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20231..21529
                     /gene="purA"
                     /locus_tag="ACHU2L_001502"
     CDS             20231..21529
                     /gene="purA"
                     /locus_tag="ACHU2L_001502"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR1356421.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21734..22159
                     /gene="nsrR"
                     /locus_tag="ACHU2L_001503"
     CDS             21734..22159
                     /gene="nsrR"
                     /locus_tag="ACHU2L_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR1356422.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22198..24639
                     /gene="rnr"
                     /locus_tag="ACHU2L_001504"
     CDS             22198..24639
                     /gene="rnr"
                     /locus_tag="ACHU2L_001504"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR1356423.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24819..25550
                     /gene="rlmB"
                     /locus_tag="ACHU2L_001505"
     CDS             24819..25550
                     /gene="rlmB"
                     /locus_tag="ACHU2L_001505"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR1356424.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         5992 a         6486 c         7185 g         5929 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagaca
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttaaa
     8101 agtgctgtaa ggcatagacc acccaagcgg gaatagctca gttggtagag cacgaccttg
     8161 ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaattcttc tctcaataaa
     8221 atatctacag cgacgcgatg cgttattgct ggtttttgtt gtctctgaca aactcttgta
     8281 aacagagtta tccacagcct caggctgtaa tcttaatttc aaagaaactt cgcacggtga
     8341 atggtatttt tttaacctat tgataaataa gttaaaaatc aagattccgt tttgtcgagt
     8401 cgatcacttg acgattttat tcgtcttgaa ttgcaatgcg tttttatttt tattcacaag
     8461 ctgtggatga atcaggcgtc acgcggtaac cctttttcaa tcacccgaac cagacgctgt
     8521 tttttcggca attgcacttc gactatgcac gcatttcgtc tctctatttg ctgcgcaatc
     8581 gcccacgcta tgtgctcatc gagaagtggg tgctcacctt tacgactttc cagcgctgtc
     8641 aaaatcgttt catcccaagg ggcattgcct aatgccacgg cgatattacg cagccagcgc
     8701 aggtgaccta tacggcgaat cgccgatcct tccgtgactt ttaaaaactt ctcttcgctc
     8761 caggcaaata actcaatgag ttccggtgcg tgtagcggct tacgcgggct gaaatcgtct
     8821 tctgtagtga gttgcgaata gcgattccac gggcagataa gctggcagtc atcgcaaccg
     8881 taaatacggt ttcccattaa cggtcgcaac tcttccggga gcgccccttc aagttcgatg
     8941 gtgagataag agatacagcg gcgagcatcg acggtatatg gctcgacgat ggcaccggtc
     9001 gggcaaatcg tcatacaggc cacgcatttg ccgcatcctt cctcgactgg ttgatccacg
     9061 ggcagcggaa tatcgaccag caattcgcct aaaaagaaga acgaaccggc ctcgcgattg
     9121 aggataagtg agtgcttacc tgtccagccg agcccagctt tttcagctaa cgggcgctcg
     9181 agaataggcg cagaatcgac aaacggtcta aaattcagcg aaacacaatg ttgctgaatc
     9241 atctcgccca gctttttgag tcggttgcgc agaagtttgt gatagtcacg gcccagcgca
     9301 taacggctaa catagccgag tttggggttt ttcagcgtgc tggcaaatgc ggcgttagca
     9361 ggaaggtaat tcatccgcac gctgattaca cgcagcgtac cgggtaataa ctcatggggg
     9421 cgagcgcgca gcataccgtg acgtgccatc cagtccattt cgccgtggta ttgtttgtcc
     9481 agccatgctt gcagtttggg ctcggactcg ctgagatcgg tatcggtaat acctacctgc
     9541 tgaaagccca gttccagccc ccactgttta attttttgcg ctaactgatt gagatcgagg
     9601 ggctctgaca tgacggacca tacaatgaag aaaaaccccg taagtatacc acacaccgtc
     9661 tggtacgccg acgatatccg ccgcggagaa cgcgaggcgg cagatgtgct ggggctcaca
     9721 ctctatgagc tgatgcttcg cgctggcgag gccgcattcc aggtgtgtcg ttcggcgtat
     9781 cctgacgccc gccactggct ggtgctgtgc ggtcatggta ataacggcgg cgatggctac
     9841 gtggtcgcgc gactggccaa agcggtcggc attgaggtca cgttgttggc ccaggagagc
     9901 gacaaaccgt tgccggaaga ggccgcgctg gcacgcgaag catggttaaa cgcgggtggc
     9961 gagatccatg cttcgaatat tgtctggccc gaatcggtag atctgattgt tgatgcgctg
    10021 ctcggtaccg gcttgcagca agcgccccgc gaatccatta gccagttaat cgaccacgct
    10081 aattcccatc ctgcgccgat tgtggcggtt gatatccctt ccggcctgct ggctgaaacc
    10141 ggcgctacgc caggcgcagt gatcaacgcc gatcacacca tcacttttat tgcgctgaaa
    10201 ctaggcttgc tcactggaaa agcgcgggat gttacaggac aactgcattt tgactcactg
    10261 gggctggata gctggctggc aggtcaggag acgaaaattc agcggttttc ggcagaacaa
    10321 ctttctcact ggctgaaacc gcgtcgcccg acttcgcata aaggcgatca cgggcggctg
    10381 gtgattatcg gtggcgatca cggcacggcg ggggctattc gtatgacggg ggaagcggcg
    10441 ctacgtgctg gtgctggttt agtccgagta ctgacccgca gtgagaacat tgcgccgctg
    10501 ctgactgcac gaccagaatt gatggtgcat gaactgacta tggactctct taccgaaagc
    10561 ctggaatggg ccgatgtggt ggtgattggt cccggtctgg gccagcaaga gtgggggaaa
    10621 aaagccctgc aaaaagttga gaattttcgc aaaccgatgt tgtgggatgc cgatgcattg
    10681 aacctgctgg caatcaatcc cgataagcgt cacaatcgcg tgatcacgcc gcatcctagc
    10741 gaggccgcac ggttgttagg ctgttccgtc gctgaaattg aaagtgaccg cttacattgc
    10801 gccaaacgtc tggtacaacg ttatggcggc gtagcggtgc tgaaaggtgc cggaaccgtg
    10861 gtcgccgccc atcctgacgc tttaggcatt attgatgccg gaaatgcagg catggcgagc
    10921 ggcggcatgg gcgatgtgct ctctggtatt attggcgcat tgcttgggca aaaactgtcg
    10981 ccgtatgatg cagcctgtgc aggctgtgtc gcgcacggtg cggcagctga cgtactggcg
    11041 gcgcgttttg gaacgcgcgg gatgctggca accgatctct tttccacgct acagcgtatt
    11101 gttaacccgg aagtgactga taaaaaccat gatgaatcga gtaattccgc tccctgatga
    11161 gcaggcaaca ttagacctgg gcgagcgggt agcgaaagcc tgcgatggcg caaccgtaat
    11221 ctatctgtat ggcgatttag gcgcaggtaa aaccaccttt agccggggct ttttacaggc
    11281 tctgggtcat cagggtaatg tcaaaagccc cacttatacg ctggtcgaac cctatacgct
    11341 cgacaactta atggtctatc actttgattt gtaccgcctt gccgatcccg aggagctgga
    11401 gtttatgggg atccgcgatt attttgccaa cgatgccatc tgcctggtgg agtggccaca
    11461 acaaggtaca ggtgttcttc ctgacccgga tgtcgaaata cacattgatt atcaggcaca
    11521 aggccgtgag gcgcgcgtga gtgcggtttc ctctgcgggt gaattgttgc tggcgcgttt
    11581 agccggttaa cctttgaaag gtggcgggat gatgtatcgc atcagaaatt ggttggtagc
    11641 gacgctgctg ctgctgtgca cgccggtggg tgccgcgacg ctctctgata ttcaggtttc
    11701 taacggtaat caacaggcgc ggataacgtt gagttttatt ggcgatcctg attatgcgtt
    11761 tagccatcaa agcaaacgca ccgtggcgct cgatatcaaa caaacgggca tgattcaggg
    11821 actgccgttg ttgttcagcg gcaataatct ggtgaaggcg attcgctctg gaacgcctaa
    11881 agatgcacaa acgctacggc tggtggtcga tcttaccgaa aacggtaaaa ccgaagcggt
    11941 gaagcggcag aatggcagca attacactgt cgtctttacg attaacgccg atgtgccgcc
    12001 accgcctcct ccgccgcccg tggttgcgaa acgcgttgaa acgcctgcgg ttgtcgcacc
    12061 gcgcgtcagc gaaccggcgc gcaatccgtt taaaacggaa agtaaccgca ctacgggtgt
    12121 tatcagcagt aatacggtaa cgcgtccggc agcgcgcgcg acggctaaca ctggcgataa
    12181 aattatcatc gctattgatg ccggacacgg cggtcaggac cctggcgcta tcggccccgg
    12241 tggtacgcgg gagaaaaatg tcaccatcgc catcgcgcgt aaattgcgta ctttgctcaa
    12301 tgacgatccg atgtttaaag gcgttttaac ccgtgacggg gattacttta tctcggtgat
    12361 ggggcgcagt gatgtggcac ataagcaaaa cgccaatttc ctcgtgtcga ttcacgctga
    12421 tgccgcaccg aaccgtagtg cgactggcgc ttccgtatgg gtgctctcta accgtcgcgc
    12481 caacagtgaa atggccagct ggctggagca gcacgagaaa ctgtcggagc tgctgggtgg
    12541 ggcgggtgat gtgctggcga acagtcagtc tgacccctat ttaagccagg cggtgctgga
    12601 tttacagttc ggtcattccc agcgggtagg gtatgatgta gcgaccagta tgatcagtca
    12661 gttgcaacgc attggcgaaa tacataaacg tcgaccagaa cacgccagcc ttggcgttct
    12721 gcgctcgccg gatatcccat cagtactggt cgaaaccggt tttatcagca acaacagcga
    12781 agaacgtttg ctggcgagcg acgattacca acaacagctg gcagaagcca tttacaaagg
    12841 cctgcgcaat tacttccttg cgcatccgat gcaatctgcg ccgcagggtg caacggcaca
    12901 aactgccagt acggtgacga cgccagatcg cacgctgcca aactaaggac gattgatgcc
    12961 aattcaggtc ttaccgccac aactggcgaa ccagattgcc gcaggtgagg tggtcgagcg
    13021 acctgcgtcg gtagtcaaag aactagtgga aaacagcctc gatgcaggtg cgacgcgtat
    13081 cgatattgat atcgaacgcg gtggggcgaa acttatccgc attcgtgata acggctgcgg
    13141 tatcaaaaaa gatgagctgg cgctggcgct ggctcgtcat gccaccagta aaatcgcctc
    13201 tctggacgat ctcgaagcca ttatcagcct gggctttcgc ggtgaggcgc tggcgagtat
    13261 cagttcggtt tcccgcctga cgctcacttc acgcaccgca gaacagcagg aagcctggca
    13321 ggcctatgcc gaaggacgcg acatggacgt gacggttaaa ccggcagcgc atccggtggg
    13381 aacgacgctg gaggtgctgg atctgttcta caacaccccg gcgcggcgca aattcctgcg
    13441 caccgagaaa accgaattta gccatattga tgaaatcatc cgccgcattg cgctggcgcg
    13501 tttcgacgtc acgatcaacc tgtcgcataa cggtaaaatt gtgcgtcagt accgcgcagt
    13561 gccggaaggc gggcaaaaag aacggcgctt aggcgcgatt tgcggcaccg cttttctcga
    13621 acaagcgctg gcgattgaat ggcaacacgg cgatctcacc ctgcgcggct gggtggccga
    13681 tccaaatcac actacgcccg cactggcgga aattcagtat tgctacgtga atggtcgcat
    13741 gatgcgcgat cgcctgatca atcatgcgat ccgccaggcc tgcgaagaca aactgggggc
    13801 cgatcagcaa ccggcatttg tgttgtatct ggagatcgac ccgcatcagg tggacgtcaa
    13861 cgtacacccc gccaaacacg aagtgcgttt ccatcagtcg cgtctggtgc atgattttat
    13921 ctatcagggc gtgctgagcg tgctacaaca gcaactggaa acgccgctac cgctggacga
    13981 tgaaccacaa cccgcaccgc gtgccatccc ggaaaaccgc gtggcggcgg ggcgcaatca
    14041 ctttgcagaa ccggcagctc gtgagccggt agctccgcgc tactctcctg cgcccgcctc
    14101 aggcagtcgt ccggctgccc cctggccgaa tgcgcagcca ggctaccaga aacagcaagg
    14161 tgaagtgtat cgccagcttt tgcaaacgcc cgcgccgatg caaaaaccaa aagcgccgga
    14221 accgcaggaa cctgcacttg cggcgaacag tcagagtttt ggtcgggtac tgactatcgt
    14281 ccattccgac tgtgcgttgc tggagcgcga tggcaacatt tcacttttat ccttgccagt
    14341 ggcagaacgt tggctgcgtc aggcacaatt gacgccgggt gaagcgcccg tttgcgccca
    14401 gccgctgctg attccgttgc ggctaaaagt ttctggcgaa gaaaaatcgg cattagaaaa
    14461 agcgcagtct gccctagcgg aattgggtat tgatttccag tcagatgcac agcatgtgac
    14521 catcagggcc gtgcctttac ccttacgcca acaaaattta caaatcttga ttcctgaact
    14581 gataggctac ctggcgaagc agtccgtatt cgaacctggc aatattgcgc agtggattgc
    14641 acgaaatctg atgagcgaac atgcgcagtg gtcaatggca caggccataa ccctgctggc
    14701 ggacgtggaa cggttatgtc cgcaacttgt gaaaacgccg ccgggtggtc tgttacaatc
    14761 tgttgattta catccggcga taaaagccct gaaagatgag tgatatcagt aaggcgagcc
    14821 tgcctaaggc gatttttttg atggggccga cggcctccgg taaaacggcg ttagccattg
    14881 agctgcgtaa aattttacca gtagagttga taagcgttga ttctgtcctt atttacaaag
    14941 ggatggatat cgggacggcg aagccgaacg ctgaagagtt actcgccgcg ccgcaccgat
    15001 tgctggatat tcgcgatccg tcgcaggctt actcggctgc tgattttcgc cgcgatgcgc
    15061 tggcggaaat ggccgatatc accgcggcgg ggcggatccc actgttagtg ggcggtacga
    15121 tgttgtattt caaggcattg ctggaagggt tgtcgccgct accgtcggca gacccggaag
    15181 tgcgggccag aattgagcaa caggcggcag agcaaggttg ggagtcattg catcgtcaac
    15241 ttcaggaggt agatccggtt gcggcagcaa ggattcatcc aaatgatcca caaaggcttt
    15301 cccgggcact ggaagttttt ttcatttcgg gtaaaacttt aacggaactg acgcaaacgt
    15361 caggagacgc tctaccgtat caggtgcatc agttcgccat cgccccggcg agccgtgaac
    15421 tgctccatca acgcattgag cagcgttttc atcagatgtt ggcttcaggt tttgaagcag
    15481 aagtccgggc gctttttgcc cgaggagatt tgcatacgga cttgccttcc attcgttgcg
    15541 tgggttatcg ccagatgtgg tcttaccttg aaggcgaaat ctcatacgat gaaatggttt
    15601 atcgaggtgt ttgcgccacg agacagttgg cgaagcggca gataacctgg ctgcgtggtt
    15661 gggaaggggt tcactggctt gacagtgaaa aaccagaaca ggcgcgtgac gaagtattac
    15721 aggttgttgg tgctatcgca ggctgaatgt gtacaattga gacgtatcgt gcgcaatttt
    15781 ttcagaatcg aaaggttcaa agtacaaata agcatataag gaaaagagag aatggctaag
    15841 gggcaatctt tacaagatcc gttcctgaac gcactgcgtc gggaacgtgt tccagtttct
    15901 atttatttgg tgaatggtat taagctgcaa gggcaaatcg agtcttttga tcagttcgtg
    15961 atcctgttga aaaacacggt cagccagatg gtttacaagc acgcgatttc tactgttgtc
    16021 ccatctcgcc cggtttctca tcacagtaac aacgccggtg gcggtaccag cagtaactac
    16081 catcatggta gcagcgcgca gaatacttcc gcgcaacagg acagcgaaga aaccgaataa
    16141 ggtttcgggc tgttttttta cacggggagc cagcgatcct gcgttccccg ctgatctatt
    16201 tagagggtta tacgcttgtt tgaccgttat gatgctggtg agcaggcggt actggtacac
    16261 atctatttta cgcaagacaa agatatggaa gacctccagg agtttgaatc tctggtctct
    16321 tccgccggtg tcgaagcatt gcaggtgatt accggtagcc gtaaagcgcc gcacccaaag
    16381 tattttgtag gtgaaggtaa agcagttgaa attgcggaag ctgtcaaagc gatgggtgct
    16441 tcggtcgttc tttttgacca tgccctgagc ccggcgcaag agcgtaacct ggagcgtttg
    16501 tgcgagtgtc gtgttatcga ccgcaccggc cttattttag atattttcgc ccaacgtgcg
    16561 cgtacccatg agggtaagtt gcaggttgag ctggcgcagc tgcgccatct ggctacgcgc
    16621 ctggtgcgtg gctggaccca ccttgaaaga cagaaaggcg ggataggttt gcgtggtccg
    16681 ggtgaaaccc agctcgaaac cgaccgtcgt ttgttgcgta atcgcatcgt gcagatacag
    16741 tcgcgcctgg aaagagttga aaagcagcgt gagcaggggc ggcaatcgcg tatcaaagcc
    16801 gacgttccta ctgtttcgct ggtgggatat accaacgccg gtaaatctac ccttttcaat
    16861 cgcatcaccg aagcgcgggt ctacgcggca gaccagttgt ttgccaccct cgacccgacg
    16921 ttgcggcgta ttgacgttgc agatgtcggt gaaaccgtac ttgcagatac cgtagggttt
    16981 attcgccacc tgccgcacga tctggtggcg gcatttaaag ccacgttaca agagacgcgg
    17041 caagccacat tactgctgca cgtcattgat gcggcggatg tgcgtgtaca agaaaacatc
    17101 gaagcggtga atacggttct tgaagagatc gacgctcacg agatcccaac cctgctggtg
    17161 atgaacaaga tcgatatgct ggaagatttc gaaccgcgta ttgatcggga cgaagagaac
    17221 aaaccgatcc gtgtctggct ttccgcacag accggagcgg ggataccaca gctttttcag
    17281 gctttgacgg agcggctttc cggcgaggtg gcgcagcata cattgcgtct gccaccgcag
    17341 gaagggcgtc tgaggagtcg tttttatcag cttcaggcaa tagaaaaaga gtggatggag
    17401 gaggacggca gcgtaagtct gcaagttcgt atgccgatcg ttgactggcg tcgcctctgt
    17461 aaacaagaac cggcgttgat cgattacctg atctaacggc gtagcgtctg aagcgtggag
    17521 tcatatcctc tggcgtcgaa agacaacagg gatcaccgca taacaaatat ggagcacaaa
    17581 catggcgtgg aatcagcccg gtaataacgg acaagaccgc gacccgtggg gaagcagcaa
    17641 acctggcggc aactctgagg gaaatggaaa caaaggcggt cgcgatcaag ggccacctga
    17701 tttagatgat atcttccgca aactgagcaa aaagctcggt ggtctgggcg gcggtaaagg
    17761 caccggatct ggcggtggca gttcatcgca aggcccgcgc ccgcagcttg gcggtcgtgt
    17821 cgttaccatc gcagcggcag cgattgtcat tatctgggcg gccagtggtt tctataccat
    17881 taaagaagcc gaacgcggcg tggtaacacg ctttggtaaa ttcagccatc tggttgagcc
    17941 gggtctgaac tggaaaccga cgtttatcga cgaagtcaaa ccggtgaacg tggaagccgt
    18001 gcgtgaactg gccgcttctg gtgtgatgct gacgtcggac gagaacgtag tgcgcgttga
    18061 gatgaacgtg cagtaccgcg tcaccaatcc ggaaaaatat ctgtatagcg tgaccagccc
    18121 ggatgacagc ctgcgtcagg ctaccgacag cgccctgcgt ggagttatcg gtaaatacac
    18181 catggaccgc attctgacgg aaggtcgtac cgtgattcgt agcgatactc agcgcgaact
    18241 ggaagagacg attcgtccgt atgacatggg tatcacgctg ctggacgtca acttccaggc
    18301 tgctcgtccg ccggaagaag taaaagcggc gtttgacgat gcgattgccg cgcgtgaaaa
    18361 cgaacagcaa tacattcgtg aagcagaagc gtattccaac gaagttcagc cgcgtgcgaa
    18421 cggtcaggcg caacgtatcc tcgaagaggc gcgtgcgtac aaggcccaga ccatcctgga
    18481 agctcagggt gaagtggcgc gctttgctaa acttctgccg gaatataaag ccgcgccgga
    18541 aattactcgc gagcgtctgt atatcgagac gatggaaaaa gtgttgggta acacccgcaa
    18601 agtgctggtt aacgataaag gtggcaacct gatggttctg ccgttagacc agatgctgaa
    18661 aggtggtaac gcccctgcgg cgaagagcga taacggtgcc agcaatctgc tgcgtctgcc
    18721 gccagcctct tcctccacaa ccagtggagc aagcaacacg tcgtccacca gtcagggcga
    18781 tattatggac caacgccgcg ccaacgcgca gcgtaacgac taccagcgtc agggggaata
    18841 acgatgcgta agtcagttat cgcgattatc atcatcgtgc tggtagtgct ttacatgtct
    18901 gtctttgtcg tcaaagaagg tgagcgcggt attacgctgc gttttggtaa ggtactgcgt
    18961 gacgatgaca acaaaccttt ggtttatgag cctggtctgc atttcaagat accgttcatt
    19021 gaaacggtga aaatgctcga cgcacgtatt cagaccatgg acaaccaggc cgaccgcttt
    19081 gtgaccaaag agaagaaaga cctgatcgtc gactcttaca tcaaatggcg catcagcgat
    19141 ttcagccgtt actacctggc aacgggtggt ggcgacattt cgcaagcgga agtgctgttg
    19201 aaacgtaagt tctctgaccg tctgcgttct gaaattggtc gcctggacgt gaaagatatc
    19261 gtcaccgatt cccgtggtcg tctgaccctc gaagtacgtg acgcgctgaa ctccggttct
    19321 gcgggtacag aagatgaagt tactaccccg gcagcagata acgccattgc cgaagcggca
    19381 gagcgcgtaa cggctgagac gaagggcaaa gttccggtca tcaacccgaa cagtatggcg
    19441 gcgctgggta ttgaagttgt cgatgtgcgt atcaagcaga tcaacctgcc gaccgaagtg
    19501 tctgaagcga tctacaaccg tatgcgcgcc gagcgtgaag cggtagcgcg tcgtcaccgt
    19561 tcacaaggtc aggaagaagc ggaaaaactg cgcgcgactg ccgactatga agtgaccaga
    19621 acgctggcag aagctgagcg tcagggccgc attatgcgtg gtgaaggcga tgccgaagca
    19681 gccaaactgt ttgctgatgc attcagtaaa gatccggact tctacgcatt catccgtagc
    19741 ctgcgtgctt atgagaacag cttctctggc aatcaggacg tgatggtcat gagcccggat
    19801 agcgatttct tccgctacat gaagacgccg acttccgcaa cgcgttaata taacgactgc
    19861 ggtacaggtc aataaagcca ccgcatcctc agggatgtcg gtggttttct ttttctataa
    19921 ggataatgaa tgaattcgac aatctggctg gcgcttgccc tggttttggt actggaaggt
    19981 ttagggccga tgctttaccc gaaggcatgg aagaagatga tctctgcgat gaccaatttg
    20041 cccgataata ttttacgtcg ttttggcggt ggacttgtgg ttgcgggcgt tgtggtctac
    20101 tacatgttga ggaaaacgat tggctgaaca aaaaacagac tgatcgaggt catttttgag
    20161 tgcaaaaagt gctgtaactc tgaaaaagcg atggtagaat ccatttttaa gcaaacggtg
    20221 attttgaaaa atgggtaaca acgtcgtcgt actgggcacc caatggggtg acgaaggtaa
    20281 aggtaagatc gtcgatcttc tgactgaacg ggctaaatat gttgtacgct accagggcgg
    20341 tcacaacgca ggccatactc tcgtaatcaa cggtgaaaaa accgttctcc atcttattcc
    20401 atcaggtatt ctccgcgaga atgtaaccag catcatcggt aacggtgttg tgctgtctcc
    20461 ggccgcgctg atgaaagaga tgaaagaact ggaagaccgt ggcatccccg ttcgtgagcg
    20521 tctgctgctg tctgaagcat gtccgctgat ccttgattat cacgttgctc tggataacgc
    20581 gcgtgagaaa gcgcgtggcg cgaaagcgat cggcaccacc ggtcgtggta tcgggcctgc
    20641 ttatgaagat aaagtggcac gtcgcggtct gcgtgttggc gaccttttcg acaaagaaac
    20701 cttcgctgaa aaactgaaag aagtgatgga atatcacaac ttccagttgg ttaactacta
    20761 caaagctgaa gcggttgatt accagaaagt tctggatgat acgatggctg ttgccgacat
    20821 cctgacttct atggtggttg acgtttctga cctgctcgac caggcgcgtc agcgtggcga
    20881 tttcgtcatg ttcgaaggtg cgcagggtac gctgctggat atcgaccacg gtacttatcc
    20941 gtacgtaact tcttccaaca ccactgctgg tggcgtggcg accggttccg gcctgggccc
    21001 gcgttatgtt gattacgttc tgggtatcct caaagcttac tcaactcgtg taggtgcagg
    21061 tcctttcccg gccgaactgt ttgatgaaac tggcgagttc ctctgcaagc agggtaacga
    21121 attcggtgca actacgggtc gtcgtcgtcg taccggctgg ctggacaccg ttgccgttcg
    21181 tcgtgcggta cagctgaact ccctgtctgg cttctgcctg actaaactgg acgttctgga
    21241 tggcctgaaa gaggttaaac tctgcgtggc ttaccgtatg ccggatggtc gcgaagtgac
    21301 taccactccg ctggcagctg acgactggaa aggtgtagag ccgatttacg aaaccatgcc
    21361 gggctggtct gaatccactt tcggcgtgaa agatcgtagc ggcctgccgc aggcggcgct
    21421 gaactacatc aagcgtattg aagagctgac cggtgtgccg atcgatatca tctctaccgg
    21481 tccggatcgt actgaaacca tgattctgcg cgacccgttc gacgcgtaat tctggtacgc
    21541 ctggcagata ttttgcctgc cgggctaaca gtgtgataca ttgctgtgtc ggttaagcca
    21601 ttacgctatc cgacacagtg ttaaatcctc gcttttttcc ttccccgaac tgaaataaat
    21661 tagcgacaca gcttgtggct ggtttatcat caatataaat gtattttttc ccgatttccc
    21721 ttttgaggtt gatgtgcagt taacgagttt cactgattac ggattacgtg cgctgatcta
    21781 catggcgtca ttgccagaag ggcggatgac cagtatttct gaagtgactg acgtctacgg
    21841 cgtctcccgt aatcatatgg tcaaaataat caatcaactt agtcgtgccg gctacgtgac
    21901 tgctgttcgt ggaaaaaatg gcggcattcg cctgggtaaa ccggcgagtg cgatacgtat
    21961 tggtgatgtg gtgcgcgagc tggagccctt atcgctggtg aattgcagca gtgagttttg
    22021 ccacattaca cctgcctgtc ggttgaaaca ggcgctttct aaggccgtgc aaagttttct
    22081 tacggaactg gataactaca cgcttgccga tttggttgaa gagaatcaac cgctttataa
    22141 attattgctg gtggagtgac gaaaatcttc atcagagatg acaacggagg aaccgagatg
    22201 tcacaagatc ctttccagga acgcgaagct gaaaaatacg cgaatcccat ccctagtcgg
    22261 gaatttatcc tcgaacattt aaccaaacgt gaaaaaccgg ccagccgtga tgagctggcg
    22321 gtagaactgc acattgaagg cgaagagcag cttgaaggcc tgcgtcgccg cctgcgcgcg
    22381 atggagcgcg atggtcaact ggtcttcact cgtcgtcagt gctatgcgct gccggaacgc
    22441 ctcgacctgg tgaaaggtac cgttattggt caccgtgatg gctacggctt tctgcgggtt
    22501 gaagggcgta aagatgattt gtatctctcc agcgagcaga tgaaaacctg cattcatggc
    22561 gatcaggtgc tggcgcagcc gctgggtgct gaccgtaaag gtcgtcgtga agcgcgtatt
    22621 gtccgcgtac tggtgccaaa aaccagccag attgttggtc gctactttac cgaagcgggc
    22681 gtcggctttg tggttcctga cgatagccgt ctgagcttcg atatcttaat cccgcccgat
    22741 cagatcatgg gcgcgcggat gggctttgtg gtcgtagtcg aactgactca gcgtccgact
    22801 cgccgcacca aagcggtggg taaaatcgtc gaagtgctgg gcgacaatat gggcaccggc
    22861 atggcggttg atatcgctct gcgtacccat gaaattccgt atatctggcc gcaggctgtt
    22921 gagcaacagg ttgcggggct gaaagaagaa gtgccggaag aagcaaaagc gggccgtgtc
    22981 gatttgcgcg atttaccgct ggtcaccatt gatggcgaag acgcccgtga ctttgacgat
    23041 gcagtttact gcgagaaaaa acgcggcggc ggctggcgtt tatgggtcgc gattgccgac
    23101 gtcagctact atgtgcgtcc gccaacgccg ctggacagag aagcgcgtaa ccgtggcacg
    23161 tcggtgtact tcccttcgca ggttatcccg atgctgccgg aagtgctctc taacggcctg
    23221 tgttcgctca acccgcaggt agaccgcctg tgtatggtgt gcgagatgac ggtttcgtcg
    23281 aaaggccgcc tgacgggcta caaattctac gaagcggtga tgagctctca cgcgcgtctg
    23341 acctacacca aagtctggca tattctgcag ggcgatcagg atctgcgcga gcagtacgcc
    23401 ccgctggtta agcatctcga agagttgcat aacctctata aagtgctgga taaagcccgt
    23461 gaagaacgcg gtgggatctc atttgagagc gaagaagcga agttcatttt caacgctgaa
    23521 cgccgtattg aacgtatcga acagacccag cgtaacgacg cgcacaaatt aattgaagag
    23581 tgcatgattc tggcgaatat ctcggcggcg cgtttcgttg agaaagcgaa agaaccggca
    23641 ctgttccgta ttcacgacaa gccgagcacc gaagcgatta cctctttccg ttcagtgctg
    23701 gcggagctgg ggctggagct gccgggtggt aacaagccgg aaccgcgtga ctacgcggaa
    23761 ctgctggagt cggttgccga ccgtcctgat gcagaaatgc tgcaaaccat gctgctacgc
    23821 tcgatgaaac aggcgattta cgatccagaa aaccgtggtc acttcggtct ggcattgcag
    23881 tcctatgcgc actttacttc gccgattcgt cgttatcctg acctgacgct gcaccgcgcc
    23941 attaaatatc tgctggcgaa agagcagggg catcagggca acaccactga aaccggcggc
    24001 taccattatt cgatggaaga gatgttgcaa ctgggtcagc actgttcgat ggcggaacgt
    24061 cgtgccgacg aagcaacgcg cgatgtcgct gactggctga agtgtgactt catgctcgac
    24121 caggtaggta acgtctttaa aggcgtaatt tccagcgtca ctggctttgg cttcttcgtc
    24181 cgtctggacg acttgttcat tgatggtctg gtccatgtct cttcgctgga caatgactac
    24241 tatcgctttg accaggtagg gcaacgcctg atgggggaat ccagcggcca gacttatcgc
    24301 ctgggcgatc gcgtggaagt tcgcgtcgaa gcggttaata tggacgagcg caaaatcgac
    24361 tttagtctga tctccagtga acgcgcaccg cgcaacgtcg gtaaaacggc gcgcgagaaa
    24421 gcgaaaaaag gcgatgcagg caaaaaaggc ggcaagcgtt gtcaggtcgg taaaaaggta
    24481 aactttgagc cagacagcgc cttccgcggt gagaaaaaaa cgaagccgaa agcggcgaag
    24541 aaagacgcga gaaaagcgaa aaagccatcg gcgaaaacgc agaaaatagc cgcagcgacc
    24601 aaagcgaagc gtgcggcgaa gaaaaaagtg gcagagtgat caataccctc tttaaaagaa
    24661 gagggttaga ttgctgacaa aatgcgcttt gttcatgccg gatgcggcgt gaacgcctta
    24721 tccggcctac ataatcacgc aaattcaata tattgcagag atcatgtagg ccggataaac
    24781 gtagtgcatc aggcaaaacg taaacaacga gtacattaat gagcgaaatg atttacggca
    24841 tccacgcagt gcaggccctg ctggagcgcg cccctgaacg ttttcaggaa gtctttattt
    24901 taaaaggccg tgaagataaa cgtctgttac cgctgattca cgcccttgag tctcagggcg
    24961 tggttatcca gttggcaaac cgccaatatc tcgacgagaa aagcgacggt gccgtgcatc
    25021 agggcattat cgcccgcgtg aagccaggac gtcagtatca ggaaaacgat ctgccggatc
    25081 tgatcgcttc gctcgatcaa ccgttcctgc tgatcctcga cggcgtaacc gatccgcaca
    25141 acctcggcgc gtgcctgcgt agtgcggacg ccgcaggcgt tcatgcggtg attgtgccga
    25201 aagatcgctc cgcacagctc aacgctacgg cgaaaaaagt agcctgcggc gcggcagaaa
    25261 gcgttccgct gattcgggtg actaaccttg cgcgcaccat gcgtatgttg caggaagaga
    25321 atatctggat cgtcggtacg gcaggcgagg cggatcatac actctatcag agcaaaatga
    25381 ccggacgcct ggcgctggtg atgggcgcgg aaggtgaagg tatgcgtcgc ctgacccgtg
    25441 aacattgcga tgagttgatc agcatcccaa tggcaggaag tgtttcttcc ctgaacgttt
    25501 cggttgcaac cggaatttgc ttatttgaag cggttcgtca gcgcagctag ttcctctgaa
    25561 ttatgaaaag ccatccagat ttggatggtt tt
//
LOCUS       DAZPEU010000040        24796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPEU010000040 DAZPEU010000000
VERSION     DAZPEU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.040"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            389..2047
                     /locus_tag="ACHU2L_001506"
     CDS             389..2047
                     /locus_tag="ACHU2L_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR1356425.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACHU2L_001507"
     CDS             complement(2044..3000)
                     /locus_tag="ACHU2L_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR1356426.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACHU2L_001508"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACHU2L_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR1356427.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACHU2L_001509"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACHU2L_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR1356428.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHU2L_001510"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHU2L_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR1356429.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACHU2L_001511"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACHU2L_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR1356430.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACHU2L_001512"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACHU2L_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR1356431.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACHU2L_001513"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACHU2L_001513"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR1356432.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="ACHU2L_001514"
     tRNA            complement(9859..9946)
                     /locus_tag="ACHU2L_001514"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHU2L_001515"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHU2L_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR1356433.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHU2L_001516"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHU2L_001516"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR1356434.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHU2L_001517"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHU2L_001517"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR1356435.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHU2L_001518"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHU2L_001518"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR1356436.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHU2L_001519"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHU2L_001519"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR1356437.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="ACHU2L_001520"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="ACHU2L_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR1356438.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHU2L_001521"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHU2L_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR1356439.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="ACHU2L_001522"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="ACHU2L_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR1356440.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="ACHU2L_001523"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="ACHU2L_001523"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR1356441.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="ACHU2L_001524"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="ACHU2L_001524"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR1356442.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
BASE COUNT         6186 a         6126 c         6679 g         5805 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaac
//
LOCUS       DAZPEU010000041        24653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPEU010000041 DAZPEU010000000
VERSION     DAZPEU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.041"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..386)
                     /locus_tag="ACHU2L_001525"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACHU2L_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACHU2L_001526"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACHU2L_001526"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR1356443.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACHU2L_001527"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACHU2L_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR1356444.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHU2L_001528"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHU2L_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR1356445.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACHU2L_001529"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACHU2L_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR1356446.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACHU2L_001530"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACHU2L_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR1356447.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACHU2L_001531"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACHU2L_001531"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR1356448.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACHU2L_001532"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACHU2L_001532"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR1356449.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHU2L_001533"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHU2L_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR1356450.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACHU2L_001534"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACHU2L_001534"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR1356451.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACHU2L_001535"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACHU2L_001535"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR1356452.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACHU2L_001536"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACHU2L_001536"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR1356453.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACHU2L_001537"
     CDS             10748..11212
                     /locus_tag="ACHU2L_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1356454.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHU2L_001538"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHU2L_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR1356455.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACHU2L_001539"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACHU2L_001539"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR1356456.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHU2L_001540"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHU2L_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR1356457.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHU2L_001541"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHU2L_001541"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR1356458.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHU2L_001542"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHU2L_001542"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR1356459.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHU2L_001543"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHU2L_001543"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR1356460.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHU2L_001544"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHU2L_001544"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR1356461.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="ACHU2L_001545"
     CDS             complement(20505..20798)
                     /locus_tag="ACHU2L_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1356462.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="ACHU2L_001546"
     CDS             complement(20795..22084)
                     /locus_tag="ACHU2L_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1356463.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="ACHU2L_001547"
     CDS             complement(22140..23078)
                     /locus_tag="ACHU2L_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR1356464.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="ACHU2L_001548"
     CDS             complement(23098..23862)
                     /locus_tag="ACHU2L_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR1356465.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24181..>24653
                     /locus_tag="ACHU2L_001549"
     CDS             24181..>24653
                     /locus_tag="ACHU2L_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR1356466.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6103 a         5950 c         6484 g         6116 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg gggcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
    24001 cgattgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
    24061 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
    24121 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
    24181 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
    24241 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat
    24301 gacaatgaaa ccgagctgat ttacgtcaag aaaggggttg caaggtttac catcgattct
    24361 tcgttatatg tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg
    24421 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
    24481 tttcaggggg ctgaggaaaa tcagttactg caaccgcatt cttgtccggt aattgccgcg
    24541 gggcagggaa aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa
    24601 agtaaaaata gccaaacatc ttcgttatgg gatgcattag cctatacatt agc
//
LOCUS       DAZPEU010000042        24563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPEU010000042 DAZPEU010000000
VERSION     DAZPEU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.042"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..507)
                     /gene="mug"
                     /locus_tag="ACHU2L_001550"
                     /pseudo
     CDS             complement(1..507)
                     /gene="mug"
                     /locus_tag="ACHU2L_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
     gene            632..707
                     /locus_tag="ACHU2L_001551"
     tRNA            632..707
                     /locus_tag="ACHU2L_001551"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:665..667,aa:Ile,seq:cat)
     gene            complement(761..1506)
                     /gene="nfeF"
                     /locus_tag="ACHU2L_001552"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             complement(761..1506)
                     /gene="nfeF"
                     /locus_tag="ACHU2L_001552"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            1783..2406
                     /gene="nfeR"
                     /locus_tag="ACHU2L_001553"
                     /gene_synonym="yqjI"
     CDS             1783..2406
                     /gene="nfeR"
                     /locus_tag="ACHU2L_001553"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR1356467.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2560..4080)
                     /gene="aer"
                     /locus_tag="ACHU2L_001554"
                     /pseudo
     CDS             complement(2560..4080)
                     /gene="aer"
                     /locus_tag="ACHU2L_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            4498..5877
                     /gene="ygjG"
                     /locus_tag="ACHU2L_001555"
     CDS             4498..5877
                     /gene="ygjG"
                     /locus_tag="ACHU2L_001555"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR1356468.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5919..6250)
                     /locus_tag="ACHU2L_001556"
                     /pseudo
     CDS             complement(5919..6250)
                     /locus_tag="ACHU2L_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHU2L_001557"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHU2L_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR1356469.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7634..10720
                     /gene="ebgA"
                     /locus_tag="ACHU2L_001558"
     CDS             7634..10720
                     /gene="ebgA"
                     /locus_tag="ACHU2L_001558"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR1356470.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            10717..11166
                     /locus_tag="ACHU2L_001559"
     CDS             10717..11166
                     /locus_tag="ACHU2L_001559"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR1356471.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11229..12662
                     /locus_tag="ACHU2L_001560"
     CDS             11229..12662
                     /locus_tag="ACHU2L_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1356472.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            12796..13867
                     /gene="ygjJ"
                     /locus_tag="ACHU2L_001561"
                     /pseudo
     CDS             12796..13867
                     /gene="ygjJ"
                     /locus_tag="ACHU2L_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            13884..16235
                     /gene="ygjK"
                     /locus_tag="ACHU2L_001562"
     CDS             13884..16235
                     /gene="ygjK"
                     /locus_tag="ACHU2L_001562"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR1356473.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATQEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            16461..18479
                     /gene="fadH"
                     /locus_tag="ACHU2L_001563"
     CDS             16461..18479
                     /gene="fadH"
                     /locus_tag="ACHU2L_001563"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR1356474.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18524..18940)
                     /gene="higA"
                     /locus_tag="ACHU2L_001564"
     CDS             complement(18524..18940)
                     /gene="higA"
                     /locus_tag="ACHU2L_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR1356475.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18937..19251)
                     /gene="higB"
                     /locus_tag="ACHU2L_001565"
     CDS             complement(18937..19251)
                     /gene="higB"
                     /locus_tag="ACHU2L_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR1356476.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(19536..20672)
                     /gene="rlmG"
                     /locus_tag="ACHU2L_001566"
     CDS             complement(19536..20672)
                     /gene="rlmG"
                     /locus_tag="ACHU2L_001566"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR1356477.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            20757..21260
                     /locus_tag="ACHU2L_001567"
     CDS             20757..21260
                     /locus_tag="ACHU2L_001567"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR1356478.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            21337..22029
                     /locus_tag="ACHU2L_001568"
     CDS             21337..22029
                     /locus_tag="ACHU2L_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR1356479.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            22108..23106
                     /locus_tag="ACHU2L_001569"
     CDS             22108..23106
                     /locus_tag="ACHU2L_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1356480.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            23377..24342
                     /gene="alx"
                     /locus_tag="ACHU2L_001570"
     CDS             23377..24342
                     /gene="alx"
                     /locus_tag="ACHU2L_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR1356481.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         5875 a         6462 c         6403 g         5823 t
ORIGIN      
        1 ttatcgccca cgcactacca gcgcctggtc cagctcgcga tacgcttcaa ccagtttctc
       61 cagtgaaacg cgacttaaac cgctgggatt tggcagcacc caaatctgcg tcgaaccaat
      121 ggtgagcgtt tgtttccccc actgtgcacc gcgctagctg aatccctgtt catatgcttg
      181 tttgcccaga atagccaacg cctgcggctg ataatcttca attttttcaa tcagcttacg
      241 cccacctgcg tgcagctcct gctttgaaac ttcattggct tgcaccgttg gacggtctac
      301 cagtttggtg atgccacaac gataatccag cagatgctgt gcctcctgcg gcttcaactg
      361 acggtcggta aacccggcct gatatatcac cttccagaag cgatttgccg gatgagcaaa
      421 gggaaaacca gtcccggcgg atgaaagccc agggttgata ccgcaaaaca cgacccgtaa
      481 ccctggagcc aaaatatcct caaccatatt tactcctttc taacatcacc aggaaaagta
      541 taacggattg aaaatacatt gtttataaaa acagcaggcg cgcggtaatg gctggattgc
      601 ggcacggagt tactttataa tccgctacca tggcccctta gctcagtggt tagagcaggc
      661 gactcataat cgcttggtcg ctggttcaag tccagcaggg gccaccagat atagcaaagg
      721 ctgacgagaa atcgtcagcc tttttctttt tatatatcag ttactttgcg tgccagtaag
      781 ccgctgcacg tacccgctgt gggtcatact gttccgcttc aaagcggcgg cttaaattct
      841 taacgacttt accttcgccg gttatccaga tgaagtaatc atcggcaggg atttgcatct
      901 gcgccagacg cgcatctacc gcctgctcat catgtgccag ccattcgata ttaaaaccat
      961 caaggtgcgc gagataatcc tgacaggcgt tatcccgcac gctaaccagc gcactaactt
     1021 gcggtttaac ggcaagtttg ctcaacgttt ccaggcggcg gcgcagtgcg ggcattccgg
     1081 attcatcgca gacatacagc tgatacgcgt aatcttccgg caccaccagc gaaccgcgcg
     1141 gacctgccac cgtaagttta tcgcccagcc gctggcgacc ccaccgtcgt gaataaagaa
     1201 atcgatcgcc agttcatggc gtagttcgtc atacagtggc gtatagtcac gcgacggtgg
     1261 gcgtggtcct tccggccaga cgatgctctc ttccgttacc gttggcggca caaagtgagc
     1321 gtcaggttga ggaaagaaga gtttgctgtg atcgtcaaag ccgcgcgatg taaaaccgtc
     1381 caacgcctcg ccgccaagga caatgcgctg aaaaccggcg ctgatgcgct caacgcgtag
     1441 cacagtcagt tcacggaagc gcagatcatt gcgaacgcgc tgcgggtagc ggggggtgtt
     1501 attcattgtt atcgccttcg tgatggtaat cagatatatc taaataaaat tcgcaaatga
     1561 taatgattgt taatcatgat aaatgcaagc gatttgtaga actgatatgt ctgaagtatg
     1621 tgaaggcatt cagtgccata gcaatgctgc cggatgcggc gtgaacgtct tatccggcct
     1681 agatgtgagg tgcttccacg tttaaaaatc atttttgtac ttgcagtaat gctcacatca
     1741 gatataaatt agatatatct aatcaagcaa aaggaggctg atatgagcca tcatcacgaa
     1801 gggtgttgta aacatgaagg ccagccacgc catgagggct gctgcaaagg tgagaagtca
     1861 gaacacgagc actgcggaca cggtcaccag catgaacacg gtcaatgttg cggtggtcgc
     1921 cacggtcgcg gcggcggtcg tcggcaacgt ttctttggtc acggtgaatt gcgtctggtg
     1981 attctggata ttctctcgcg cgatgacagc cacggttacg aattgattaa agcgattgag
     2041 aatctaaccc aggggaatta caccccaagc ccgggcgtca tctacccgac gctggatttt
     2101 ctgcaggagc agtcgctgat taccatccgc gaagaggaag gtggtaagaa gcagattgcg
     2161 ctgaccgaac aaggcgcgca gtggctggaa gaaaaccgcg aacaagtgga gatgattgaa
     2221 gaacgcatca aagcgcgttg cgttggcgcg gcgctgcgcc agaacccgca aatgaagcgg
     2281 gcgctggata attttaaagc ggtgctggat ttacgcgtca accagagcga tatcagtgat
     2341 gcacaaataa aaaagatcat tgcggtgatc gaccgcgccg cttttgatat tacgcaactg
     2401 gattaatcgc cgcatccgcc agtggcgcgg tgcaattgcc ggatgcgacg cttgacgcgc
     2461 cttatccggc ctacacccgc tacacacccc gcaggcctga taagatgcgc cagcatcgca
     2521 tcaggcattg tgctccaacc gccggatccg gcataccgat taatgcagca ccgtcaccgc
     2581 gtcttccagt cggctggcgc ggtgtttcac catcgccgac acctgcgcac tctcttccac
     2641 cagctcggca tttttctggg tgatcaggtt aagctcatcc actgcacggg tcaggctgga
     2701 aagcccatcg gcctgttcca gcgttgaatg gctaatctgg gcgatcaact gggtgacgtt
     2761 tttcacctgt gccacaatat cttccatcgt ccgtccggcg gcgtgtacct gctgcgaacc
     2821 ggattgcacc ttatcagcac tggcatcaat cagcttgcga atatcgttgg cagcattagc
     2881 gctgcggctg gctaaatgac gcacttcccc tgccaccact gcaaaacctt tgccctgttc
     2941 gccggcacgc gccgcttcca ccgccgcatt cagggccaga atattggtct gaaacgcaat
     3001 atcgttaatc agcgaagtaa tggtgccaat gcgctaggta ctgtcggcga tatcgtccat
     3061 tgtcttgatc accgtggtca tcgcctcccc accctgcact gccgcattac tggcggtgat
     3121 agaaagttta tcggcagccg acgccgtggc agagttctgt ttcaccgacg ccgccatttg
     3181 gttcatggtc gccaccgttt gctgaacgtt atcaactgtc tgctgggtat gttcgttcag
     3241 ttcatcggtg cctttcgcca gcgtctcact gccatttctg acactggaca cctggcttga
     3301 gacatcgtta attagccaac ggcacatcag gccaagctgc cctaccgcgc gtaatgtcag
     3361 ccccagctca tcgctgcgat tcaggtgctc aacactatta cgctctccgg tcgccacctt
     3421 cagtgcctga cgggcgacat tttctatcgg gcgcacaatc tgccattcaa aacaagcgct
     3481 tgccaacaat accactaaca cacagaggaa atacgtcacc accggggcag caacaaacca
     3541 aagcatggcc gccagcaaga taaacatcag ggtcatcact ccacgcgtcc gccagcgaag
     3601 cggtaatgaa ggcagtttac ccagccagcc tttacgcacc accaggcctt tatgaatacg
     3661 cttactggta cgtccggagt tcaacgcttt gtacagcggc tccaccgccg caatctcttc
     3721 atccgtcgcc cgggtacgaa tcgacatata gccactgatt tttccctcgc gcaccatcgg
     3781 taccgcattg gcccgcaccc aataatggtc gccatttttg cggcgatttt tcacgatgcc
     3841 gctccagggc tccccttttt tcagggtgaa ccacatatcc gcaaacgccg cttttggcat
     3901 atccgggtga cgcaccatgt tgtgcggctg cccttgtaac tcttgcaagg taaagccgct
     3961 cacctgcaca aaagtgtcat tagcatgagt gatatagctt tgcagatcgg tagtggacat
     4021 cagagtggta tcgtccgcca gcggggtatt ttgctgggtg acatacggat gagaagacat
     4081 gttcgcgtcc tgtgcaggtt atatggttgt taacttcttg tcagagttta tgtcggcccc
     4141 gctgcggtta tctttaaccg attaatttga tttagatcgc aatttgcgat taaagcacaa
     4201 atctaattct ttgatttaaa atactttcac tctgttacta tacgaaaacg ttaattatct
     4261 tgcccaaaaa tcaggcaatt attgccctga aaacgtgcat ttgcgcagca atcatcaaat
     4321 ccatacccga caaaaaccgt gcaaaataac aacaaatgtt aacggatagc attaaatatt
     4381 gcacaaatga taaccgcatt tgtgtttatc ccgattttcg cgatcgcagc cggagtggcg
     4441 caatccctgc aatacttaaa tcggtatcat gtgatacgcg agcctccgga gcatattttg
     4501 aacaggttac cttcgagcgc atcggcttta gcgtgcagcg cccacgccct gaatctcatt
     4561 gagaagcgaa cgctggatca tgaggagatg aaagcactta accgagaggt gattgaatac
     4621 ttcaaagagc atgtcaatcc ggggttttta gagtatcgca aatctgttac cgccggcggg
     4681 gattacggag ccgtagagtg gcaagcggga ggtttaaata cgcttgtcga cacccaggga
     4741 caggagttta tcgactgcct gggaggtttt ggaattttca acgtggggca ccgtaatcca
     4801 gttgtggttt ccgccgtaca gaatcaactt gcgaaacaac cactgcacag ccaggagcta
     4861 ctcgatccgt tacgggctat gctggcgaaa acccttgctg cgctaacgcc cggtaaactg
     4921 aaatacagct tcttctgtaa tagcggcacc gagtccgttg aagcggcgct gaagctggcg
     4981 aaggcttatc agtcgccgcg cggcaaattt acttttattg ccaccagcgg cgtgttccac
     5041 ggtaaatcac ttggcgcgct gtcggccacg gcgaaatcga ccttccgcaa accgtttatg
     5101 ccactgctgc cagggttccg tcatgtgccg tttggcaata tcgaagccat gcgcacggct
     5161 cttaatgagt gcaaaaaaac cggtgatgat gtggctgcgg tgatcctcga accgattcag
     5221 ggcgaaggtg gcgtaattct gccgccgccg ggctatctca ccgccgtgcg taagctgtgc
     5281 gatgagttcg gcgcactgat gatcctcgat gaagtacaaa cgggcatggg gcgcacgggc
     5341 aagatgtttg cctgcgaaca tgagaacgtt cagccggaca ttctgtgtct ggcaaaagcc
     5401 ctcggcggcg gcgtgatgcc gattggcgcg accatcgcca ctgaagaggt gttttcagtt
     5461 ctgttcgaca acccattcct gcataccacc acctttggcg gcaacccgct ggcctgtgcg
     5521 gcggcgctgg cgaccatcaa tgtgttgctg gagcagaact taccggctca ggctgagcaa
     5581 aaaggcgata tgttgctgga cggtttccgt caactggcgc gggaatatcc cgatctggta
     5641 caggaagcgc gtggtaaagg gatgttgatg gcgattgagt ttgttgataa cgaaatcggc
     5701 tataactttg ccagcgagat gttccgccag cgcgtactgg tggccggaac gctcaataac
     5761 gccaaaacga tccgcattga accgccactg acactgacca ttgaacagtg tgaactggtg
     5821 atcaaagcgg cgcgtaaggc gctggcggcc atgcgagtaa gtgtcgaaga agcgtaatat
     5881 caaatcggat ggcgatacga cgtcgccatc cgatcttttt atacgacgcg tacgcccgca
     5941 ggcatcatcc gctccggcgt taacaacacg ctttcactgc cgtcatcggt ttccgcacac
     6001 aacagcatgc attccgacgt ttcaccacgc attttcgctt tttgcagatt gcataagacc
     6061 accaccgttt tccccatcag ctcctcttcg ctgtagtacg gcaccaggct ggtcacggtt
     6121 tgcagtgttt tttccccaca tcaacctgta cgatgtacag cttgtcggcg ttttcatggc
     6181 gtttcacttc cacaatcttt ccgacgcgca tttccagacg tgcaaaatca gcgtaagcca
     6241 cggtttccat tgcttcctcc cttcgagtaa aattttacta aactatagaa aagtttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcctaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caagatattt
     7261 cgcttatcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgttc ggcaatcatt tttgccggac agtgctgccg
     7501 tttattttcg tgatccagtt aaagtaaatg catttacctg ctgcttttta gtaaaaattt
     7561 tactaaaccc ccagcaatta cacaaactac catcaccatg aatggttccg atttctctct
     7621 accgggaggc cctatgaatc gctgggaaaa cattcagctc acccacgaaa accgacttgc
     7681 gccgcgtgcg tacttttttt catatgattc tgttgcgcaa gcgcgtacct ttgcccgcga
     7741 aaccagcagc ctgtttctgc ccttaagcag tcagtggaat ttccactttt ttgaccatcc
     7801 gctgcaagta ccagaagcct tcacctctga gttaatggct gactgggggc atattaccgt
     7861 ccccgccatg tggcaaatgg aaggtcacgg caaactgcaa tataccgacg aaggttttcc
     7921 gttccccatc gatgtgccgt ttgtccccag cgataaccca accggtgcct atcaacgtat
     7981 tttcaccctc agcgacggct ggcagggtaa acagacgctg attaaatttg acggcgtcga
     8041 aacctatttt gaagtctacg ttaacggtca gtatgtgggt ttcagcaagg gcagtcgcct
     8101 gaccgcagag tttgacatca gcgcgatggt taaaaccggc gacaacctgt tgtgtgtgcg
     8161 cgtgatgcag tgggcggact ctacctacgt ggaagaccag gatatgtggt ggtcggcggg
     8221 gatcttccgc gatgtttatc tggtcggaaa acacctaacg catattaacg atttcactgt
     8281 gcgtaccgac tttgacgaag cctattgcga tgccacgctt tcctgcgaag tggtgctgga
     8341 aaatctcgcc gcctcccctg tcgtcacgac gctggaatat accctgtttg atggcgaacg
     8401 cgtggtgcac agcagcgcca ttgatcattt ggcaattgaa aaactgacca gcgccagctt
     8461 tgcttttact gtcgaacagc cgcagcaatg gtcagcagaa tccccttatc tttaccatct
     8521 ggtcatgacg ctgaaagacg ccaacggcaa cgttctggaa gtggtgccac aacgcgttgg
     8581 cttccgtgat atcaaagtgc gcgacggtct gttctggatc aataaccgtt atgtgatgct
     8641 gcacggcgtc aaccgtcacg acaacgatca tcgcaaaggc cgcgccgttg gaatggatcg
     8701 cgtcgagaaa gatctccagt tgatgaagca gcacaatatc aactccgtgc gtaccgctca
     8761 ctacccgaac gatccgcgtt tttacgaact gtgtgatatc tacggcctgt ttgtgatggc
     8821 ggaaaccgac gtcgaatcgc acggctttgc taatgtcggc gatatcagcc gtattaccga
     8881 cgatccgcag tgggaaaaag tctacgtcga gcgcattgtt cgccatatcc acgcacagaa
     8941 aaaccatccg tcgatcatca tctggtcgct gggcaatgaa tccggctatg gctgtaacat
     9001 ccgcgcgatg taccatgcgg cgaaagcgct ggatgacacg cgactggttc attacgaaga
     9061 agatcgcgat gctgaagtgg tcgatattat ttccaccatg tacacccgcg tgccgctgat
     9121 gaatgagttt ggtgaatacc cgcatccgaa gccgcgcatc atctgtgaat atgctcatgc
     9181 gatggggaac ggaccgggcg ggctgacgga gtaccagaac gtcttctata agcacgattg
     9241 cattcagggt cattatgtct gggagtggtg cgaccacggg atccaggcac aggacgacca
     9301 cggcaatgtc tggtataaat tcggcggcga ctacggcgac tatcccaaca actataactt
     9361 ctgtcttgat ggtttgatct attccgatca gacgccggga tcgggcctga aagagtacaa
     9421 acaggttatc gcgccggtaa aaatccacgc gctgactcgc ggcgagttga aagtcgaaaa
     9481 taaactgtgg tttaccacgc ttgatgacta caccctgcac gcagaggtgc gcgccgaagg
     9541 tgaaacgctc gcgacgcagc agattaaact gcgcgacgtt gcgccgaaca gcgaagcccc
     9601 cttgcagatc acgctgccgc agctggacgc ccgcgaagcg ttcctcaaca ttacggtgac
     9661 caaagattcc cgcacccgct acagcgaagc cgggcattct atcgccactt atcagttccc
     9721 gctgaaggaa aacaccgcgc agccagtgcc tttcgcacca aataatgcgc gtccgctgac
     9781 gctggaagac gatcgtttga gctgcaccgt tcgcggctac aacttcgcga tcaccttctc
     9841 aaaaatgagt ggcaaaccga catcctggca ggtaaatggc gagtcgctgc tgacccgcga
     9901 gccaaagatc aacttcttca agccgatgat cgacaaccac aagcaggagt acgaagggtt
     9961 gtggcagccg aatcatttgc agatcatgca ggaacatctg cgcgactttg ccgtagaaca
    10021 gagcgatggt gaagtgttga tcatcagccg cacagttatt gcaccgccgg tgtttgactt
    10081 cgggatgcgc tgtacctaca tctggcgcat cgctgccgat ggccaggtta acgtggcgct
    10141 ttccggcgag cgttacggcg actatccgca catcattccg tgcatcggtt tcaccatggg
    10201 gattaacggc gaatacgatc aggtggcgta ttacggtcgt ggaccgggcg aaaactacgc
    10261 cgacagccag caggctaaca tcatcgatat ctggcgcagc accgtcgatg ccatgttcga
    10321 gaactatccc ttcccgcaga acaacggcaa ccgtcagcat gtccgctgga cggcactgac
    10381 taaccgccac ggcaacggtc tgctggtggt tccgcagcgc ccaattaact tcagcgcctg
    10441 gcactatacc caggaaaaca tccacgctgc ccagcactgt aacgagctgc agcgcagtga
    10501 tgacatcacc ctgaacctcg accaccagct acttggcctc ggctccaact cctggggcag
    10561 cgaggtgctg gactcctggc gcgtctggtt ccgtgacttc agctacggct ttacgttgct
    10621 gccggtttct ggcggagaag ctaccgcgca aagcctggcg tcgtatgagt tcggcgcagg
    10681 gttcttttcc acgaatttgc acagcgagaa taagcaatga ggatcatcga taacttagaa
    10741 cagttccgcc agatttacgc ctctggcaag aagtggcaac gctgcgttga agcgattgaa
    10801 aatatcgaca acattcagcc tggcgtcgcc cactccatcg gtgattcatt gacctaccgt
    10861 gtggaaaatg actccgcgac cgatgcgcta tttaccgggc atcgacgcta ttttgaagtg
    10921 cattactacc tgcaagggca gcaaaaaatt gaatatgcgc cgaaagagac attacaggta
    10981 gtggaatatt atcgtgatga aactgaccgt gaatatttaa aaggctgcgg agaaaccgta
    11041 gaggtccacg aagggcaaat cgttatttgc gatatccatg aagcgtatcg gtttatctgc
    11101 aataacgcgg tcaaaaaagt ggttctcaaa gtcaccatcg aagatggtta tttccataac
    11161 aaataacaac tacggcggca aaaggagttt gccgccaccg ctaccctaca cattttcgga
    11221 gatgtgttat gtctgatacc aaacgtaata caatcggcaa attcggcttg ctctcgctga
    11281 cttttgccgc cgtttacagc tttaacaacg ttatcaataa taatattgag cttggactgg
    11341 cctcggcacc gatgtttttc ctcgcgacga ttttttattt tattcccttc tgtctgatca
    11401 tcgcagaatt tgtttcgtta aataaaaact cagaagccgg tgtctacgcg tgggtaaaaa
    11461 gttcgctggg cggacgttgg gcatttatta ctgcctacac ttactggttc gtaaatctgt
    11521 tctttttcac ctcgctgttg ccgcgcgtta ttgcttatgc ttcgtatgcc ttcctcggct
    11581 acgaatatat tatgacgccg gttgccacca ccgttatcag tatggtgctg ttcgccttct
    11641 ccacctgggt ttccaccaac ggggcgaaaa tgctggggcc aattacctcc gtcacttcaa
    11701 cgctgatgct gctgttaacg ctctcctaca ttttactggc aggtacggca ctggttggcg
    11761 gcgtacagcc tgccgatccg atcaccgttg acgcgatgat cccgaacttc aactgggcgt
    11821 tcctcggcgt taccacctgg atctttatgg ccgcaggtgg cgcggagtcc gtcgcggtgt
    11881 acgttaacga cgtcaaaggc ggttcgaaat cgttcgttaa agtgatcatc ctcgccggga
    11941 tttttatcgg cgtactgtat tccgtctcct cggtgctgat taacgtcttc gtcagcagca
    12001 aagagttgaa atttaccggc ggatcggtgc aggtattcca cggcatggcg gcgtattttg
    12061 gtctgccgga agcgttgatg aatcgctttg tcggtctggt gtcctttacc gcaatgttcg
    12121 gttccctgct gatgtggacc gcaacgccgg tgaaaatttt cttctccgaa atcccggaag
    12181 gcatctttgg taagaaaacc gtcgaactta acgaaaacgg cgttccggcg cgcgcagcat
    12241 ggatccagtt tctgatcgtc atcccgctga tgattatccc gatgctcggt tccaataccg
    12301 tgcaggatct gatgaatacc attattaata tgaccgccgc agcgtccatg cttccgccgt
    12361 tattcatcat gctggcttac ctgaatttac gcgccaaatt agatcacctg ccacgcgatt
    12421 tccgtatggg ctcccgccgc accggtatta tcgttgtttc aatgctgatt gcgatatttg
    12481 ccgtagggtt tgtcgcttcg acattcccga ctggcgcgaa tattctgacc atcatttttt
    12541 ataacgtcgg cggtattgtt atcttcctcg gctttgcgtg gtggaaatac agcaaatata
    12601 taaagggatt aacggctgaa gagcgccata ttgaagcgac gccagccagc aatgttgatt
    12661 aagcagacta ataaaaaatg agcaggcatt ttccctcttc ctggatgagg agagggaaaa
    12721 tagtttctgc ctttatattt ttataattac aacgataaaa ggctgtactt tttctttagc
    12781 tcatggatta acacaatgaa attaatcact gcaccatgca gagcattact tgctctgccg
    12841 ttttgctacg ccttttctgc ggcaggagaa gaagcacgtc cggcagaaca tgacgacaca
    12901 aaaacacccg caattacctc gacatcttct ccttcatttc gtttttacgg cgaattaggg
    12961 cttggtggat atatggattt agagggtgag aataaacata aatacagcga cggcacctat
    13021 attgaaggtg gcctggagat gaagtacggc tcctggttcg gcctgattta cggcgaaggc
    13081 tggaccgtgc aggccgacca cgacggtaat gcctaggtgc ctgaccatag ctggggtggt
    13141 ttcgaaggtg ggattaaccg tttctatggc ggttatcgta ccaatgatgg caccgaaatc
    13201 atgctcagtc tgcgtcagga ttcctcgctg gatgacctgc aatggtgggg cgacttcaca
    13261 cccgatttgg gctacgtcat tcccaatacc cgcgacatta tgaccgcgct gaaggtacag
    13321 aacttaagcg gcaactttcg ttatagcgtc accgccacac ctgccggaca tcatgacgaa
    13381 agcaaagcct ggctacattt tggcaagtac gatcgctacg acgacaaata cacctatccg
    13441 gcaatggatg aacggttaca tccagtatga ccttgccgaa ggcatcacct ggatgaatgg
    13501 tctggaaatt accgacggca caggacaact ctatctcacg ggcctgttta cccctaactt
    13561 tgccgctcgc gcctggcacc ataccggacg tgccgacggg ctggacgtac cgggaagtga
    13621 aagcgggatg atggtgagcg ccatgtatga agcgttaaaa ggcgtttatc tctccaccgc
    13681 ttacacctac gccaaacatc gccctgacca cgctgacgat gaaaccacct ctttcatgca
    13741 gtttggtatc tggtacgaat atggcggcgg acgtttcgcc acggcttttg atagccgctt
    13801 ctacatgaaa aatgcctcta acgatcccag cgaccaaatc ttcctgatgc aatatttcta
    13861 ctggtaataa ggactgtaat ctcatgaaaa tcaaaactat tttaacgcca gtaacctgcg
    13921 ctctgctgat aagtttttcc gctcatgccg ctaacgccga caattataaa aacgtgatta
    13981 accgtactgg tgcgccgcag tacatgaagg attacgatta cgacgatcac cagcgtttta
    14041 atccgttttt cgatctcgga gcctggcatg gtcatctgtt gccagacggc ccgaacacca
    14101 tgggcggctt tccgggcgtt gcgctgctga cggaagaata catcaacttt atggccagca
    14161 atttcgaccg cctgactgtc tggcaagacg gcaagaaagt caacttcacg ctggaggcat
    14221 acagtattcc cggcgcgctg gtgcaaaaac tgacagcaaa agatgtgcag gtcgaaatga
    14281 ctctgcgctt cgccacgccg cgcacgtcac tactggaaac taaaatcatc agcaataaac
    14341 cgctggatct ggtgtgggat ggcgaactgc tggaaaaact ggaagcgaaa gaagggaaac
    14401 cgctttccga taaaaccatt gctggcgaat accctgacta tcagcgcaaa atcagcgcca
    14461 cccgtgatgg cctgaaagtc acctttggca aagtgcgcgc cacctgggat ctgctgacct
    14521 ccggcgaatc ggaatatcag gtgcataaat ccctgccggt gcagactgaa atcaacggta
    14581 atcgctttac cagtaaggcg catatcaacg gttcgaccac gctctacacc acctattccc
    14641 atctgctgac cgctcaggaa gttagcaaag agcaaatgca gatccgcgat attctggcgc
    14701 gtccggcgtt ttatctcacc gcctcgcagc aacgctggga agaatacctg aagaaagggt
    14761 taaccaatcc ggatgcgacg caggaacaga cgcgcgtcgc ggtgaaagcg atcgaaacgc
    14821 tcaacggtaa ctggcgctca ccgggcggtg cggtgaatta taacaccgtc acgccgtcgg
    14881 tgaccggacg ctggttctcc ggcaatcaga cctggccgtg ggatacctgg aagcaggcgt
    14941 ttgcgatggc gcatttcaat ccggatatcg ccaaagagaa tatccgcgcg gtcttctcct
    15001 ggcagatcca gcctggcgac agcgtgcgtc cgcaggatgt gggctttgtc cccgacctga
    15061 tagcgtggaa ccttagcccc gagcgtggtg gcgatggcgg caactggaac gaacgtaata
    15121 ccaaacccag ccttgccgcc tggtcggtga tggaagtata caacgtcacc caggataaag
    15181 cctggttggc agagatgtac ccgaaactgg tggcctatca cgactggtgg ttacgtaacc
    15241 gcgatcataa cggcaacggg gtgccggaat atggcgcgac ccgcgacaaa gcccacaaca
    15301 ccgagagcgg cgagatgctg tttacggtga aaaaaggcga taaagaagag acgcagtctg
    15361 ggctgaacaa ctacgcccgc gtggtggaga aaggccagta cgacagtctg gaaattccgg
    15421 cacaggtcgc tgcgtcgtgg gaatcggggc gtgatgatgc cgccgtcttt ggttttatcg
    15481 acaaagaaca gttggataaa tatgtcgcta atggcggcaa acgtagcgac tggacggtga
    15541 aattcgccga aaaccgcagt caggacggaa cgttgctggg ctactcgcta ttgcaggagt
    15601 cggtggatca ggccagctat atgtacagcg ataaccatta tctggcggag atggcaacca
    15661 tcctcggtaa gccagaagag gccaaacgct atcgccagtt ggcacagcag ctcgcggact
    15721 acatcaacac ctgtatgttc gacccgacta cccagttcta ctatgacgta cgtattgaag
    15781 ataaaccact ggcgaacggc tgcgcgggca aaccgattgt tgagcgcggt aaagggccgg
    15841 aaggctggtc gccgctgttt aacggtgcgg caacgcaggc caatgccgac gcggtggtga
    15901 aggtgatgct cgatcctaaa gagttcaaca catttgttcc gctgggaacg gcggcgttaa
    15961 ccaatccggc ttttggcgct gatatctact ggcgcgggcg cgtatgggtg gatcagttct
    16021 ggtttggtct gaaagggatg gagcgttacg gttatcgtga tgatgccctg aagctggcgg
    16081 atacgttctt caggcacgcc aaaggattaa ccgccgatgg cccaattcag gagaattaca
    16141 acccgctgac aggcgcgcag caaggcgcac caaatttctc ctggagtgcc gctcatttgt
    16201 atatgttgta taacgatttt ttccgtaagc agtaatttgc aacggctggc ggatgcggcg
    16261 tgaacgcctt atccgcccta catgtgcaat cccccaagcc ggataaaaca cggcaagcgt
    16321 cgcatccggc gttaccaccg ggcgtattct ttttgaatcc catcacaaac cccgcactcc
    16381 ccttttccct tttctccatc gacggctaaa ttagaactca tccgactaca taacaattat
    16441 tttacatact ggacaatttt atgagctacc cgtcgctgtt cgccccgctg gatttaggtt
    16501 ttaccacgtt aaaaaaccgc gtgttgatgg gttcaatgca caccgggctg gaggaatacc
    16561 cggacggtgc tgaacggctg gctgcgtttt atgccgaacg cgcccgtcac ggcgtggcgc
    16621 tgattgtcag cggcggcatt gcaccagatt taacaggcgt tggcatggaa ggtggcgcaa
    16681 tgctcaacga cgccagccag atcccacacc atcgcaccat taccgaagcc gtacatcagg
    16741 aaggcggcaa aatcgccctg caaattttgc ataccgggcg ctacagctac caaccgcatc
    16801 tggtcgcacc gtccgcattg caggccccca tcaaccgttt cgttcctcac gaactcaccc
    16861 atgaagagat cctgcaactg atcgacgatt tcgcccgctg cgcgcaactg gcgcgggagg
    16921 ccggatacga tggcgtcgaa gtgatgggct ctgaagggta tttgatcaac gaatttctga
    16981 cgctgcgcac caatcagcgt agtgaccagt ggggcggcga ttaccgcaac cggatgcgat
    17041 ttgccgtaga agtagtgcgt acggtgcgcg aacgcgtcgg caacgacttc attattattt
    17101 accggttgtc gatgctcgac ctagtcgaag gcggcggcac ttttgccgaa acggtagaac
    17161 tggcgcaggc cattgaagcg gcgggcgcga ccattatcaa caccggaata ggctggcacg
    17221 aagcgcggat tccgaccatc gccacgcccg tgccgcgcgg cgcatttagc tgggtcacgc
    17281 gcaaactgaa aggccacgtc tcgctgccgc tggtaaccac caaccggatt aacgatccgc
    17341 aggttgccga cgatattctc tcgcgcggcg atgccgatat agtatcgatg gcgcgaccgt
    17401 ttctcgcgga tgcggaactg ctgtcaaaag cgcaatcagg gcgagccgat gagatcaaca
    17461 cttgtattgg ctgcaaccag ggctgcctcg atcagatctt cgttggcaaa gtcacctcgt
    17521 gcctggtgaa tcctcgcgcc tgccacgaaa ccaaaatgcc aatccttccc gccgtgcaga
    17581 aaaaaaatct ggcggtggtc ggcgctggac ctgctgggct ggcgtttgcc attaacgcgg
    17641 cggcgcgtgg gcatcaggta acattgtttg atgcgcacag cgagatcggc gggcagttta
    17701 atatcgccaa acagatcccc ggcaaagagg agttttacga aacgctgcgc tactaccgcc
    17761 ggatgatcga agtgacgggc gtgacgctga aactcaatca caccgtgacg gcggatcagt
    17821 tacaggcgtt cgatgaaacg atcctcgcca gtgggatcgt gccgcgtgtt ccgcctatcg
    17881 acgggatcga tcatccgaag gtattgagtt atctcgatgt actgcgcgac aaagcgccgg
    17941 taggcaacaa ggttgccatc atcggttgtg gcgggattgg ttttgatacg gcgatgtatt
    18001 taagtcagcc gggcgaatcc accagccaga atatcgccag gttctgtaat gagtggggga
    18061 tcgacagcag cctacaacag gctggtggct taagcccgca gggaatgcag atcccgcgta
    18121 gcccacggca aattgtgatg ctccagcgca aagccagcaa accgggacag gggttaggca
    18181 aaaccaccgg ctggatccat cgcaccaccc tgctctcgcg gggtgtgaaa atgatcccag
    18241 gcgtaagtta tcagaagatt gacgatgacg ggctgcatgt ggtgatcaac ggcgaaacgc
    18301 aggtattagc agtggacaat gtggtgatct gcgcagggca agagccaaac cgcgcgctgg
    18361 cgcaaccgct gattgatagc gggaaaactg tgcatttaat tggcggctgc gatgtggcta
    18421 tggagctgga cgcacgacgg gcaattgccc agggaacacg gctggcgctg gagatttaaa
    18481 tcgttatttg aagcgccgga tgcaacgcat tcggcacgtc gcgttaatca ataaacaagg
    18541 cgggagaaat gccgaatcgc gttgccaatt ttttagcgtg ttccagcgtt aatttcctct
    18601 tcccgctcaa aacgcgtgac accatagatt tactgccaat ttccggcaga tcggaaaggg
    18661 ttaaaccata ttgatccata agggtacgaa tcacggctat accgccaggc atggcttgag
    18721 ccatggcatt aaattccgca aattcgggcg ctgattcttc ccacgcggtt attttggcac
    18781 acaccagatc cagcaagggg ttttcaggat cgttgagcag cagatgatct accagttcca
    18841 gcgcctgggt gtattgttcc tcgttctgaa tacccgccag aaaaggtgcc acagcagtta
    18901 gcttttctcc tgcttgcaag atgtcggcaa tcgcaatcat tttttcccct tagtacgatg
    18961 aacagcggta aagaaatcgt attctttatg cgtcataact tcacgtatgt agcacttttg
    19021 cgattcaaaa aagaccattg ctacaacacg taattcattg cccccaacat tgaaaacata
    19081 atgcttatcc agatatttga agttatccag agatgggaat actgctttta atgactcagg
    19141 ttttttgaaa tatcccttag caatcgtgtt ccccagagcc accaactccg ttttatgttg
    19201 cgggtatttt tccgcagcat ctttcaatgc tttttgagtt atcaggtgca ttcttcatca
    19261 cgtccgttga caaattggca atatgataac atccgttgac agattggcaa cggatgaatt
    19321 tatctgtgtg acggaagaat aaacgtagaa aaacgctaaa caagagaatg tagaggatgg
    19381 tgacagggat tttttgtttt atggaagcgc gattccagaa ctgagcagac attgccggat
    19441 gcgacgcttg ccgcgtctta tccggcctgg gggtaacgtg ctgaacgtag gccggataag
    19501 gcgtttacgc cgcatccggc aggtaactcc caatcttacc gacgacgtcc cagcttcacc
    19561 gctttcagca ccacaaattt attattcgtc gcaatagtgg tgcagttgcc gaaaattttc
    19621 ttcagtttat ggaagtaatc cagatggcgg ttggcaacga tatacagctc gccgttgatt
    19681 ttcaggcagc ggcgggcgta gtggaacatc tcccaggcga cgttatcggt cagcgcatgt
    19741 tgttggtgaa acggagggtt gcagagcaca gcattaaagc ggaaaggctc cacgccggag
    19801 agcgcgttgt tgatcataaa ctcgctgcga tccaacgcct ctggcatgtt ggtttcaacg
    19861 ttcaaacggc tggaagcaac cgccatcggc gattcatcga caaacaccac tttcgcctgc
    19921 gggtttttat caagcagcgt cagaccaata acgccattac cgcaaccgag atcgacaatc
    19981 tccccttcga gattctctgg cagatgttgc ataaagaagc gcgcgccgat atcaagcccg
    20041 gtgcgggaga agacattcgc atggttatgg atagtccagt cagtaccttc cagcttccag
    20101 ctaacggtct gcggcgcatc ggccagcggc ggttcattga acgtgcaatt aatcaggcgc
    20161 gcttttttcc atgccagcgt ggtggtggtc gggccgagca ctttttcgaa cagttccagc
    20221 gtggaagtgt gaatgtcacg ggctttggca ccggcaataa tacgtgtatc cgacgtgacc
    20281 actttgcgca gcgcacgcag ttgctgttcc agcaatgcca gtgttttcgg cactttgatc
    20341 agcaccacac ccggctgttg cgggtagtcg gcggtgctgt cgagaaactt cacgctcgac
    20401 tcatcaatcc cgtttaggcg taaattctcg cgcgtcgcca gttcactgat gtatgagtcg
    20461 ccaatgctgt acggcttatg ttccgccagc gcgcagctta acgcaccaaa ggcatcattc
    20521 aggatcaaca ccgggccgag gatttctgtg tcgtccaact gttgcagcaa atattcatct
    20581 gccgcttccc acgcctgtag cgggttaacg tcatccgtcg ccggaaaacg ttgtagtgtc
    20641 agtgaacgga aaccgttgtc taagtggctc atcggccctc ctgaatgata aaatttcgac
    20701 gttgcccctg aaaagggtgc gtgagtatac cctttttctc tttttcgtgg gcggttatga
    20761 gcaatcttac ttatcttcag ggttatcccg agcagctgct ttcgcaggtg cgaacactga
    20821 ttaacgaaca gcgtctgggc gatgtgctgg caaaacgcta tccgggaaca cacgactacg
    20881 ccaccgataa agctctctgg caatatactc aggatctgaa aaatcagttt ctgcgtaatg
    20941 ccccgccgat caataaagtg atgtatgaca ataagatcca tgtgctaaaa aacgcgctcg
    21001 ggttacatac cgctgtttct cgtgtgcagg gcagcaagct gaaagcaaaa gcagagatcc
    21061 gcgtcgctac cgtgtttcgc aacgcgccgg aaccgttttt gcgcatgatc gtggtgcacg
    21121 agctggcgca cctgaaggag aaagagcata acaaagcgtt ctatcaattg tgctgccata
    21181 tggagccgca gtaccaccag cttgagttcg acacccggct gtggctaacg cagttgtcgc
    21241 ttgggcagga caaaatctga aaatgatgtc aggcgttatc ttttctttct ttagcgccta
    21301 aaatcgacct cccccctttc gttaaggata acgaccatgc tgcgcgcatt tgcccgcctt
    21361 cttctccgta tttgtttttc tcgccggact ctaaagattg cctgcttact gttacttgtg
    21421 gcaggagcga ccatttttat cgccgatcgc gttatggtca acgccagcaa gcagctgacc
    21481 tggggcgatg tcaacgccgt cccggcgcgt aacgtgggtt tattgctggg ggcgaggccg
    21541 ggtaatcgct actttacccg acgtattgat accgcagcag cgttgtacca cgccggaaaa
    21601 gtgaaatggc tactggtgag tggcgataac ggacgtaaaa attacgatga agcatcaggt
    21661 atgcagcagg ctttgatcgc taaaggcgta ccggcgaaag tgatcttttg cgactatgcc
    21721 ggattctcaa cgctggattc ggtagtgcgt gccaaaaagg tgtttgggga aaaccatatc
    21781 accattatct cgcaagaatt tcataaccaa cgcaccatct ggctggcaaa acagtacggc
    21841 atcgacgcta tcgggtttaa cgcgccagac ctcaatatga agcacggttt ttatactcaa
    21901 ctgcgggaga aactcgctcg tgtcagcgcg gtgattgacg caaaaatcct ccatcgccag
    21961 ccgaaatatc tcgggccatc ggtcatgatt gggccgttta gtgagcatgg ctgtccggcg
    22021 aaagaataat gcgtatctgc gcacgtcgaa gatgaaaaag gcgtgctaca ttgacgacag
    22081 aatcccttta tggagtatcc acgcgttatg atacgtttcg ctgtgattgg tacgaactgg
    22141 atcactcgcc agttcgtcga ggccgcccat gagagcggta aatacaagtt aaccgccgta
    22201 tattcccgca gccttgagca ggctcagcac ttcgccaatg atttttctgt cgagcatctg
    22261 tttacgtcgc tggaagcgat ggcggaaagc gatgccattg acgcggtgta tattgccagc
    22321 ccgaattccc tgcacttttc ccagacacaa cttttcctta gccataaaat tcatgtgatt
    22381 tgcgaaaaac cgctggcgtc gaatctggcg gaagtggatg ccgccattgc ctgtgcgcgc
    22441 gaaaatcagg tggtgctgtt tgaggcattt aaaaccgcct gcctgccgaa ctttcatttg
    22501 ttgcgccagg cgctgccgaa agcaggcaaa ctgcgtaaag tctttttcaa ctattgccag
    22561 tattcctcgc ggtatcaacg ttacctggac ggtgagaatc ccaacacctt taatccggca
    22621 ttctctaacg gttcaattat ggatatcggc ttttactgcc tggcgtcggc ggtggcgtta
    22681 tttggtgagc cgaaaagcgt gcaggcaacg gcgagtttgc tggcaagcgg cgttgacgcc
    22741 cacggcgtgg tggtgatgga ttacggtgat ttcagcgtca ccttgcagca ctcaaaggtc
    22801 agtgattctg tcctgggaag cgagattcag ggcgaagcgg gatcgctggt gattgaaaaa
    22861 ctgtctgaat gccagaaagt ctgctttgtg ccgcgtggca gccagatgca ggatctcacc
    22921 cagccgcagc atattaatac catgctctac gaagcagagc tgttcgctac cctggtggat
    22981 gagcatctgg tggatcatcc agggctggcg gtcagtcgca tcacagccaa actgctgacc
    23041 gagatccgcc gccaaactgg ggtgattttt ccggcagata gcgtaaaact atacttgcca
    23101 aagtaaaaca gtgtaaaagg tatgtaacag accattgact gactgaatgg tctgtcatac
    23161 tttgttacct gcaaagggga gtaacttcat tgccggtcga tcgtcattac gatgagtgaa
    23221 aaaccacatc cggtcaccgg gcaacccgaa aggaatacgc agacgtattc cttttttgtt
    23281 gtaagtgaga ccttgccgga aggcgaggtc tatgcataaa aagcagcggc tgacgtcttc
    23341 cgacgttggc cgttttttta tgtgtaagga acttctatga atactgtcgg cacgccgttg
    23401 ctatggggcg gattcgctgt tgttgtcgcc attatgctgg ctatcgacct gttgttgcag
    23461 gggcgtcgtg gggcacatgc catgaccatg aaacaggctg cggcctggtc gctggtctgg
    23521 gtgacgctgt cgttactgtt taacgccgct ttctggtggt atctggtgca aaccgaaggt
    23581 cgcgccgttg ccgatccaca ggccctggcc tttctcacgg gttatctgat tgagaaatcg
    23641 ctggcggtcg ataacgtctt tgtctggctg atgttgttca gctatttctc cgttccggcg
    23701 gcattacaac gtcgcgtgct ggtgtatggc gtgctcgggg cgattgttct gcgtaccatc
    23761 atgatcttca caggcagctg gctgatttcg cagttcgact ggatcctgta tatcttcggt
    23821 gccttcctgc tgtttaccgg cgtgaagatg gcgctcgccc atgaagatga atcaggcatt
    23881 ggcgacaagc cgctggtgcg ctggctacgc ggtcatttgc gcatgaccga caccatcgac
    23941 aacgagcatt tctttgtgcg taagaatgga ttgctgtacg ccacaccgct gatgctggta
    24001 ctgattctgg tggaattgag cgacgtgatt tttgccgtgg atagcattcc ggctatcttc
    24061 gccgtgacca ctgacccgtt cattgtgctg acctcaaacc tgtttgcaat cctcggcctg
    24121 cgtgcgatgt atttcctgct ggcgggcgta gcagagcgtt tctcgatgct caaatatggc
    24181 ctggcggtga ttctggtgtt tatcggtatc aagatgctga ttgtcgactt ctaccatatt
    24241 ccaatcgccg tctcgctggg cgtggtgttt ggcattctgg tgatgacgtt tattatcaac
    24301 gcctgggtga attatcggca tgataagcag cggggagaat gattcgctgt atattaagcc
    24361 attcctagag ataactcatt ccagacaaag gtgagctatt aattatgtaa tcggcttata
    24421 gctctttaca aaaagaccgc acgggtgttg gtggcgtcaa aatcgccgaa gcgttccctg
    24481 gaggccgggg cgagggcgca tcttacaggg acgttacctt gcgcccgacc cggtagccgc
    24541 aagggatagg ctgagggaac cgc
//
LOCUS       DAZPEU010000043        24338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPEU010000043 DAZPEU010000000
VERSION     DAZPEU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.043"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            133..1215
                     /locus_tag="ACHU2L_001571"
     CDS             133..1215
                     /locus_tag="ACHU2L_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR1356482.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1704..2502)
                     /locus_tag="ACHU2L_001572"
                     /pseudo
     CDS             complement(1704..2502)
                     /locus_tag="ACHU2L_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2512..3063)
                     /gene="ybcL"
                     /locus_tag="ACHU2L_001573"
     CDS             complement(2512..3063)
                     /gene="ybcL"
                     /locus_tag="ACHU2L_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR1356483.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3232..3564)
                     /gene="emrE"
                     /locus_tag="ACHU2L_001574"
     CDS             complement(3232..3564)
                     /gene="emrE"
                     /locus_tag="ACHU2L_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR1356484.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(3909..4223)
                     /gene="fliE"
                     /locus_tag="ACHU2L_001575"
     CDS             complement(3909..4223)
                     /gene="fliE"
                     /locus_tag="ACHU2L_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR1356485.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4437..6096
                     /gene="fliF"
                     /locus_tag="ACHU2L_001576"
                     /pseudo
     CDS             4437..6096
                     /gene="fliF"
                     /locus_tag="ACHU2L_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            6089..7084
                     /gene="fliG"
                     /locus_tag="ACHU2L_001577"
     CDS             6089..7084
                     /gene="fliG"
                     /locus_tag="ACHU2L_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR1356486.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7077..7763
                     /gene="fliH"
                     /locus_tag="ACHU2L_001578"
     CDS             7077..7763
                     /gene="fliH"
                     /locus_tag="ACHU2L_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1356487.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7763..9136
                     /gene="fliI"
                     /locus_tag="ACHU2L_001579"
     CDS             7763..9136
                     /gene="fliI"
                     /locus_tag="ACHU2L_001579"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR1356488.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9158..9499
                     /gene="fliJ"
                     /locus_tag="ACHU2L_001580"
                     /pseudo
     CDS             9158..9499
                     /gene="fliJ"
                     /locus_tag="ACHU2L_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            9496..10623
                     /locus_tag="ACHU2L_001581"
     CDS             9496..10623
                     /locus_tag="ACHU2L_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR1356489.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            10728..11192
                     /gene="fliL"
                     /locus_tag="ACHU2L_001582"
     CDS             10728..11192
                     /gene="fliL"
                     /locus_tag="ACHU2L_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR1356490.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11197..12201
                     /gene="fliM"
                     /locus_tag="ACHU2L_001583"
     CDS             11197..12201
                     /gene="fliM"
                     /locus_tag="ACHU2L_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR1356491.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12198..12611
                     /gene="fliN"
                     /locus_tag="ACHU2L_001584"
     CDS             12198..12611
                     /gene="fliN"
                     /locus_tag="ACHU2L_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR1356492.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12614..12979
                     /gene="fliO"
                     /locus_tag="ACHU2L_001585"
     CDS             12614..12979
                     /gene="fliO"
                     /locus_tag="ACHU2L_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR1356493.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            12979..13744
                     /gene="fliP"
                     /locus_tag="ACHU2L_001586"
                     /pseudo
     CDS             12979..13744
                     /gene="fliP"
                     /locus_tag="ACHU2L_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            13754..14023
                     /gene="fliQ"
                     /locus_tag="ACHU2L_001587"
     CDS             13754..14023
                     /gene="fliQ"
                     /locus_tag="ACHU2L_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR1356494.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14032..14817
                     /gene="fliR"
                     /locus_tag="ACHU2L_001588"
     CDS             14032..14817
                     /gene="fliR"
                     /locus_tag="ACHU2L_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR1356495.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15102..15726
                     /gene="rcsA"
                     /locus_tag="ACHU2L_001589"
                     /pseudo
     CDS             15102..15726
                     /gene="rcsA"
                     /locus_tag="ACHU2L_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(15770..15958)
                     /gene="dsrB"
                     /locus_tag="ACHU2L_001590"
     CDS             complement(15770..15958)
                     /gene="dsrB"
                     /locus_tag="ACHU2L_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR1356496.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16121..16352
                     /gene="yodD"
                     /locus_tag="ACHU2L_001591"
                     /pseudo
     CDS             16121..16352
                     /gene="yodD"
                     /locus_tag="ACHU2L_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            16650..17465
                     /locus_tag="ACHU2L_001592"
                     /pseudo
     CDS             16650..17465
                     /locus_tag="ACHU2L_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(17462..19156)
                     /gene="dgcQ"
                     /locus_tag="ACHU2L_001593"
                     /pseudo
     CDS             complement(17462..19156)
                     /gene="dgcQ"
                     /locus_tag="ACHU2L_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(19327..19509)
                     /locus_tag="ACHU2L_001594"
     CDS             complement(19327..19509)
                     /locus_tag="ACHU2L_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR1356497.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(19588..20505)
                     /locus_tag="ACHU2L_001595"
     CDS             complement(19588..20505)
                     /locus_tag="ACHU2L_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR1356498.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            20678..21598
                     /gene="yedA"
                     /locus_tag="ACHU2L_001596"
     CDS             20678..21598
                     /gene="yedA"
                     /locus_tag="ACHU2L_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR1356499.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21587..22057)
                     /gene="vsr"
                     /locus_tag="ACHU2L_001597"
     CDS             complement(21587..22057)
                     /gene="vsr"
                     /locus_tag="ACHU2L_001597"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR1356500.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22038..23456)
                     /gene="dcm"
                     /locus_tag="ACHU2L_001598"
     CDS             complement(22038..23456)
                     /gene="dcm"
                     /locus_tag="ACHU2L_001598"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR1356501.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23523..24024)
                     /locus_tag="ACHU2L_001599"
                     /pseudo
     CDS             complement(23523..24024)
                     /locus_tag="ACHU2L_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(24103..>24338)
                     /gene="drpB"
                     /locus_tag="ACHU2L_001600"
     CDS             complement(24103..>24338)
                     /gene="drpB"
                     /locus_tag="ACHU2L_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR1356502.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         6092 a         6272 c         6053 g         5921 t
ORIGIN      
        1 aaaataggga tctacttcac aatttaaatg agaaactaaa acttacatct tgaaataatc
       61 tcattgattg gatgaatatt tgtagtgcag tgacatcatt tttaaataat agttcaaata
      121 aaagggctca cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcagtactg
      181 atggcgatgt ctgctcaggc agcagagatt tacaataaag acagtaacaa gctggatctg
      241 tacggaaaag ttaatgctaa gcactacttc tcttctaacg atgcagatga tggcgacaca
      301 acttatgttc gtctgggctt caaaggcgaa actcaaatca acgatcaact gactggtttc
      361 ggtcagtggg aatatgaatt caaaggcaac cgtgctgaat ctcaaggttc ttccaaagat
      421 aaaacccgtc ttgccttcgc tggcctgaaa ttcggtgact acggctccat cgattatggc
      481 cgtaactacg gtgtagcata cgatatcggt gcatggaccg acgttctgcc agaattcggt
      541 ggcgatacct ggacccaaac agatgtgttc atgactggtc gcaccacagg tgttgcaacc
      601 tatcgtaaca acgacttctt tggtctggtt gatggtctga actttgctgc tcagtatcag
      661 ggtaaaaatg accgcactga cgtaactgaa gctaacggtg atggtttcgg tttctccact
      721 acttatgagt atgaaggatt cggtgtaggt gcaacctatg ctaaatctga ccgcactaat
      781 gatcaggtta tctacggtaa caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg
      841 gcagctggtc tgaaatatga tgcgaacaac atctatctgg ctaccaccta ttctgaaacc
      901 cagaacatga ctgtttttgg taataaccat attgccaaca aagcacaaaa cttcgaagta
      961 gttgcacaat atcagttcga cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa
     1021 ggaaaagact tgggtgcgtg gggtgatcag gacctgattg aatatattga tgtaggtgca
     1081 acctattact tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa cctgattgat
     1141 aagagcgatt tcacgaaagc atctggcgtt gctaccgatg atatcgttgc tgtaggattg
     1201 gtttaccagt tctaatctga ttactcaaag atatgttgtg ggaggctttg cctccccaac
     1261 atataagtgg cgctctcaag ccactttcct tagaagcact atcttgcttc tgactatata
     1321 aattaccctt tattttctgg gacgtttcaa cgccccattt ttaataatac tcagtgaaca
     1381 attaacgtat caattagatt taataacaac gatatccgta cctaacctga tatctaatgt
     1441 cataagcatc cctttaatta tcccctcaac ctcttgtaaa tttcccactc ttgagctcca
     1501 catagcccaa acgccggatg caaaaaaaat catacgtgca tctctgaaat tgtgatggag
     1561 gaagtgagta tcaattatat gtgcttataa acgttcccga ttggcacaga agtcaccgga
     1621 ttgttcaggc tccaataggt tgagtatgtc gtatttattt tccaaaataa aaatcgatgc
     1681 tgttgctcct taataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt
     1741 atattaaaac gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc
     1801 ctggcaatct cgctgatggg ttttactgtc gtttgtatag caggacaacg cattatttaa
     1861 cctcacgtcg tccaatatac tttgaaaact tattccctcg cttgccagac ggcgatgcaa
     1921 tgtggaaaca gaaatgtaga gatatctcgc taccttgttt gctgtccatt ttgttctagg
     1981 ttcggatagc attaggttat aacaacgact tattaatgat tgtttactat ctgataaaag
     2041 taaacaatta acatgatgtg ctcctaatga aagtagaacg cccattgcta agtgttcctg
     2101 aatttgcgtt gagaagcctc gggaaataga tgtttttagt tgctcccaac aatatattaa
     2161 ctcggggttc tgaggtaagc agaaacctgt cgtgttacga atttgatcag ttatcgcata
     2221 aaatttttga aacttttcaa ttagatcaat gggataataa agcatttctg caagataaag
     2281 acctgcttct ggataattca caatataaaa ttcatatcca cagggaaata atattatctg
     2341 attattatca acagttagac tatgcgtctc ccaattgata actttttttc cctgacggat
     2401 acgacataaa gctggcataa gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg
     2461 tatttcttca atctttaggt tagacttatc tcttgccagc atactcttac cctattttat
     2521 ctcataaact ggtgttatct cagcggttgc aattttattg gcattaagca tataaccaac
     2581 taacgctccg ctggaattag aatccacagg aatcttatca gtttttagag cccatacttt
     2641 aaactggtaa tgatggggtt tatctccttt gggaggacat gctccaccaa atcctgcata
     2701 accaaaatca tttcggcctt gaacagcacc agtcggaagt tttgtaccat cacgtcttcc
     2761 tgcatcagtg ggcaaatatg ttacagttgc tggaatatta gcaacagtcc agtgccacca
     2821 tccactgcct gtaggagcat ctggatcata cacagttacg gcaaagcttt tggtgccttc
     2881 aggagcacca gaccaggtta atgagggcga tgtattacca ccttcacacc caaatccaga
     2941 aaatacatga gacgttgtaa gttgctctcc tgtttttatt tcattactag tgacctgaaa
     3001 tgctgcggcc tgcgcagaaa atgttatgaa tgccaataca cttgaaacga taagtttttt
     3061 cataaaaacc tcattgttgt gacctatcgt tattttattt gatgtgcctt gatctcatta
     3121 tgcataaagg cgcaatgatc gcgcaaatgc aatcataatt ataaaggtgc cccgaacatt
     3181 agccataata tgcacaaatc agattcctgt tatctcaagg agaattattt tttaatgcgg
     3241 tgcacttcgt gacaacaagt taatgactaa cacaccggca caaatcaaca tcatgcctat
     3301 gatagctggc aggtccagtc gttgaccaaa aaatccccag gaaagcaaac taatcagaac
     3361 gataccgact cctgaccaga tagcataagc aatccctgta ggtatataag ccagcgtctg
     3421 agctaataac cagaatgatg cacaatagca aataattgtg ccaacagagg gccataaccg
     3481 tgtaaacccc tctgaaaact tcattaaggt tgtaccaatg acctctgcaa gtattgcgcc
     3541 accaagataa atataagggt tcatagcata ttctttcctg ttcatactgg agagaattgt
     3601 actacagttt gaacacaact caccgttttc atcctggtgt atgtgtatcc aaggggctct
     3661 ggcgtggcaa gagataaagg caggaacaat aatgatcaga aggaacccca ggctaacgaa
     3721 tgagttcact cttcctggga gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg
     3781 tggatggcag aaaacaacca ggatcaggta tccattttaa accagaaatt gagtgagttt
     3841 gccccatcca tgccccacgc tgtgggatcg gatataatta aacaagcata gtcaacaggt
     3901 tacacaacct acacctgcat ggacatcact tcctgatacg ccgccaccag cttattacgc
     3961 acctgaatcc ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt
     4021 agcgccacgc cgggttcacc gagggtgaat ttttctgcct gcgtgcgggc gactgtttgt
     4081 gtatcgctta tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgc
     4141 ggcagtgatt cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct
     4201 tcaatcccct gtatcgctga cattttcgtc tcccggataa tttctggtaa caaagcctac
     4261 cagtaagtca ataagacaaa ggcgctaaat agcaacaaaa aaactggttt attggcggat
     4321 agaaaaaacg aaagcacaaa taatgggagc gtcaattttt cgagtgtgct gacccgagag
     4381 tgagtcttgt tccactttgc caataacgcc gtccataatc agccacgaag tgcgcgatga
     4441 atgcgactgc agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga
     4501 aaattccatt gattgttacc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt
     4561 gggcgaaagc ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg
     4621 caattgtcag ccaactgacg caaatgaata ttccttaccg cttcagcgaa gccagcggcg
     4681 ctattgaggt tccggcagat aaagttcacg aactgcgcct ggcgcaacaa ggtttgccca
     4741 aaggcggcgc ggtcggtttc gaactgctcg atcaggaaaa gtttggtatc agccagttca
     4801 gcgaacaggt gaattatcag cgggcgctgg aaggcgagct ttctcgtacc atcgaaacta
     4861 tcggcccggt aaaaggggcg cgcgtacatc tggcaatgcc gaaaccgtct ttattcgtcc
     4921 gtgaacaaaa atccccttct gcatcggtga cggtaaatct gttacccggc cgcgcactcg
     4981 atgaagggca aattagcgct attgtacatc tggtttccag cgcggttgct ggtctgccgc
     5041 ctggaaacgt cacgctggtg gatcagggcg ggcatctgtt aacccagtcc aataccagcg
     5101 ggcgcgatct taatgacgct cagttgaaat atgccagcga tgtcgaaggc cgtattcagc
     5161 agcgtattga agcgatcctg tcacctattg ttggtaacgg taatattcac gcctaggtca
     5221 cggcgcaact ggacttcgcc agtaaagaac aaacggaaga acagtatcgc cctaacggtg
     5281 atgaatctca tgcggcgctt cgttcacgcc agcttaatga gagcgagcaa agcggttccg
     5341 gttatccggg cggcgtaccg ggggcgttgt cgaatcaacc ggcacctgcg aataacgcgc
     5401 caatcagcac gcctccgaca aatcaaaata accgccagca gcaggcgagc accaccagca
     5461 atagtgggcc gcgtagcaca cagcggaatg aaaccagtaa ctacgaagtc gatcgcacca
     5521 ttcgtcatac caaaatgaac gtgggcgatg tgcaacgtct gtcagtcgcg gtagtggtga
     5581 attacaaaac cttgccagac ggtaaaccat tgcctctcag caacgaacag atgaagcaaa
     5641 ttgaagctct gacccgcgag gcgatgggct tttctgaaaa acgcggtgat tcgctcaatg
     5701 ttgttaactc gccgttcaat agcagtgacg aaagcggcgg agcactgccg ttttggcaac
     5761 agcaagtgtt tatcgatcag ttgctggctg ccggtcgctg gttgctggtt gctggtactg
     5821 ctggtggcgt ggttgctgtg gcggaaagcg gtacgcccgc agctaacacg tcgcgccgag
     5881 gcggtgaaga ctgtgcagca acaggcgcag gcccgcgagg aagtggaaga tgcggtggaa
     5941 gtccgtctga gcaaagacga acaactccaa caacggcgcg ctaaccaacg actgggggca
     6001 gaagtcatga gccagcgtat ccgtgaaatg tctgataacg atccgcgcgt ggtggcgctg
     6061 gtcattcgcc agtggataaa taacgatcat gagtaacctg acaggcaccg ataaaagcgt
     6121 catcctgctg atgaccattg gcgaagaccg ggcggcagag gtgttcaagc acctctccca
     6181 gcgcgaagtg caaaccctga gcgctgcaat ggcgaacgtc acgcagatct ccaacaagca
     6241 gctaaccgat gtgctggcgg agtttgagca agaagctgaa cagtttgctg cactgaatat
     6301 caacgccaac gattatctgc gttcggtatt ggtcaaagct ctgggtgaag aacgtgccgc
     6361 cagcctgctg gaagatattc tcgaaacccg cgataccgcc agcggtattg aaacgctcaa
     6421 ctttatggag ccgcagagcg ccgccgatct gattcgcgat gagcatccgc aaattatcgc
     6481 caccattctg gtgcatctga ggcgcgccca ggccgccgat attctggcgt tgttcgatga
     6541 acgtctgcgc cacgacgtga tgttgcgtat cgccaccttt ggcggcgtgc agccagccgc
     6601 gctggccgaa ctgaccgaag tactgaatgg cttgctcgac ggtcagaatc tcaagcgcag
     6661 caaaatgggc ggcgtgagaa cggcagccga aattatcaac ctgatgaaaa cgcagcagga
     6721 agaagccgtt attaccgccg tgcgtgaatt cgacggcgag ctggcgcaga aaatcatcga
     6781 cgagatgttc ctgttcgaga atctggtgga tgtcgatgat cgcagcattc agcgtctgtt
     6841 gcaggaagtg gattccgaat cgctgctgat cgcgctgaaa ggggccgagc agccactgcg
     6901 cgagaaattc ctgcgcaata tgtcgcagcg tgccgccgat attctgcgcg acgatctcgc
     6961 caagcgtggt ccggtgcgtc tgtcgcaggt cgaaaacgaa cagaaagcga ttctgctgat
     7021 tgtgcgccgt cttgccgaaa ctggcgagat ggtgattggc agcggcgagg atacctatgt
     7081 ctgataatct gccgtggaaa acctggacgc cggacgatct cgcgccacca ccggcagagt
     7141 ttgtgccaat ggtcgagtcg gaagaaacca ttattgaaga ggccgaaccc agccttgagc
     7201 agcaactggc gcaactgcaa atgcaggccc acgagcaggg ttatcaggcg gggattgccg
     7261 aaggtcgcca gcaaggtcac gaacagggct atcaggaagg actggcccag gggctggaac
     7321 aaggtctggc agaggcgaag gctcaacaag cgccaattca tgcccggatg cagcaactgg
     7381 tcagcgaatt tcaaactacc cttgatgcgc ttgatagtgt gatagcttcg cgcctgatgc
     7441 agatggcgct ggaggcagca cgtcaggtga tcggtcagac accaacggtg gataactcgg
     7501 cactgatcaa acagatccaa cagttgttgc agcaagaacc gttattcagc gggaaaccac
     7561 agctgcgcgt acacccggat gatctgcaac gtgtggatga tatgctcggc gcaactttaa
     7621 gtttgcatgg ctggcgcttg cggggcgatc ccaccctcca ccctggcgga tgtaaagtct
     7681 ccgccgatga aggcgatctc gacgccagtg tcgccactcg ctggcaagaa ctctgccgtc
     7741 tggcagcacc aggagtggtg taatgaccac gcgcctgact cgctggctaa ccacgctgga
     7801 taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt cgctacgggc gattaacccg
     7861 cgctaccggg ctggtgctgg aagccaccgg attacaattg ccgctcggcg caacctgtgt
     7921 cattgagcgc cagaacggca gcgaaacgca cgaagtagaa agcgaagtcg ttggctttaa
     7981 cggtcaacgg ctgtttttaa tgccgctgga ggaagtggaa ggtgtcctgc ccggcgcgcg
     8041 tgtatatgcc aaaaacattt cggcagaagg gctgcaaagc ggcaagcagt tgccgctcgg
     8101 tccggcgtta ttaggtcgtg ttctggacgg tagcggtaaa ccgctcgatg gcctgccctt
     8161 ccccgatacg acggaaaccg gtgcgctgat taccccgcca tttaacccgt tgcaacgtac
     8221 accgattgaa catgtgctgg acaccggcgt gcgcccaatc aatgccctgc ttaccgtcgg
     8281 acgcgggcag cgtatggggc tgtttgccgg gtcgggcgtt ggtaaaagtg tgctgctggg
     8341 gatgatggca cgctacaccc gcgccgatgt cattgtcgtg ggtttgattg gtgaacgtgg
     8401 gcgcgaagta aaagatttta ttgagaatat cctcggtgcc gaaggacgtg cacgctcagt
     8461 ggtgattgcc gctccggcgg atgtttctcc gctcctgcga atgcaaggtg ccgcctatgc
     8521 cacgcgcatt gccgaagatt ttcgcgatcg tggtcagcat gtgttgctga ttatggactc
     8581 cctcacccgc tacgcgatgg cccagcgtga aattgcgctg gcgattggcg aaccacctgc
     8641 cactaaaggt tatccaccgt cggtgtttgc caaattaccg gcactggtcg agcgtgccgg
     8701 taatggcatt agcggcggcg gctcgattac cgcgttttat accgtgctca ccgaaggcga
     8761 tgaccagcag gaccccattg ctgactccgc gcgggcaatc ctcgacggtc acattgtgct
     8821 gtctcgccga ctggcggaag ccgggcacta tccggctatc gatattgaag cgtcgatcag
     8881 tcgcgcaatg acggcgttga tcagtgagca acattacgcg cgagtgcgca ccttcaaaca
     8941 gctgttgtcg agttttcagc gtaaccgcga tctggttagc gtcggcgcgt atgccaaagg
     9001 cagcgatccg atgctcgata aagccatcgc cctgtggccg cagctggagg gctatttgca
     9061 acaaggcatt tttgaacgcg cggactggga agcgtctctc caggggctgg agcgtatttt
     9121 cccgacagtg tcataaccca ggagataacg gcagatggcg gaagaacagc tcaaaatgtt
     9181 gatcgattat cagaatgaat atcgcaataa cctcaatagc gatatgagtg ccgggatgac
     9241 cagcaaccgc tggatcaact atcagcagtt tatccagacg ctggaaaaag ccattactca
     9301 gcatcgccag caacttaatc agtggacgca gaaagttgac attgccctga acagctggcg
     9361 agaaaagaaa caacgtttgc aggcctggca gacactgcag gaacggcaat ccacggcggc
     9421 actgcttgca gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc agcgcgccgc
     9481 catgaggaaa cctgaatgat tcgcttagcg cccttgatta ccgccgacgt tgacaccacc
     9541 acattgcctg gcggcaaagc cagcgatgct gcacaagatt ttctcacgtt gttgagcgaa
     9601 gcattagcag gcgagacaac aactgacaaa gcggcccccc agttgctggt agcaacagat
     9661 aagcccacga caaaaggcga gccgctggtc agcgatattc ttgccgatgc gcaacaagcg
     9721 gatttactga tccctgtgga tgaaacactt cctgtcatca acgacgaaca atccacatca
     9781 acaccgttaa ccaccgctca gacgatgacg ttggctgcgg tggctgacaa aaatacgaca
     9841 aaagacgaaa aagcggatga tcttaatgaa gacgtcaccg caagtctgag cgcccttttt
     9901 gcgatgttgc cgggttttga caatacgccc aaagtgactg atgcaccgtc aaccgtgtta
     9961 ccggcagaga aaccaacgct cttcacaaaa ctgacttctg cgcaactcac tacagcacag
    10021 cctgatgacg cccccggcac accagctcag ccattaacac cgctggtagc agaagcccag
    10081 agtaaagcgg aagtcatcag cacgccttcg ccggtgaccg ctgacgccag cccgctaatc
    10141 actccacacc agacacagcc actgcccacc gtcgccgcgc ctgttttgag tgcaccgctg
    10201 ggttctcacg aatggcaaca atcattgagc cagcatattt cgctgttcac ccgccagggg
    10261 caacaaagtg cagagttgcg tctgcaccca caggatttag gtgaagtgca aatttccctc
    10321 aaagtggatg ataaccaggc gcagatccag atgatttcac cgcatcagca cgtacgcgcc
    10381 gccctggaag cagcgctgcc ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta
    10441 gggcaaagca acattagtgg cgaaagcttt agtggtcagc agcaggccgc ttcccagcaa
    10501 cagcaaagcc aacgcacagc aaaccatgaa cctctggcgg gggaagacga cgatacgctt
    10561 ccggttcccg tctctttaca agggcgtgta acaggtaaca gcggcgttga tattttcgcc
    10621 taacgtcaga ggtagcaccg taatccgcgt cttttccccg ctttgttgcg ctcaagacgc
    10681 aggataatta gccgataagc agtagcgaca caggaagacc gcaacacatg actgattacg
    10741 cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat tctggtattc attaccctcg
    10801 cggcctgtgc cagcgcaggt tacagctact ggcattcgca tcaggttgcc gctgacgaca
    10861 aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc gctggatacc tttacggtca
    10921 atttgggcga tgcggatcgc gtgctttata tcggcataac cctgcgactg aaagatgaag
    10981 caacccgctc gcggctgagt gagtatttgc cggaagtccg tagtcgcttg ctgttactgt
    11041 tttcacgtca ggatgctgcc gtactggcga cagaagaagg caagaaaaac ctgattgccg
    11101 agattaaaac cacactttcc accccgcttg ttgccgggca accgaaacag gatgtcaccg
    11161 acgtgctgta taccgctttt attctgcgat aacgacatgg gcgatagtat tctttctcaa
    11221 gctgaaattg atgcgctgtt gaatggtgac agcgaagtca aagacgaacc gacagccagt
    11281 attagcggcg aaagtgacat tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc
    11341 gaacgtttgc aggcgctgga aatcattaat gagcgctttg cccgccattt tcgtatgggg
    11401 ctgttcaacc tgctgcgccg tagcccggac attaccgtcg gggccatccg cattcagccg
    11461 taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc tgaacctaat ccacctgaaa
    11521 ccgctacgcg gcacagggct ggtagtgttc tcgccgagtc tggtgtttat cgccgtggat
    11581 aacctgtttg gcggcgatgg acgcttcccg accaaagtgg aaggtcgcga gtttacccat
    11641 actgaacagc gcgtcatcaa ccgtatgttg aagctggcgc tggaaggcta tagcgacgcc
    11701 tggaaagcga ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca ggtggaattt
    11761 accaatatca ccacctcgcc gaacgacatt gtggttaaca cgccgttcca tgtggagatt
    11821 ggcaacctga ccggcgaatt taatatctgc ctgccattca gcatgatcga gccgctacgg
    11881 gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat
    11941 aacctggtgc gccaggtgca gcattcacag ctggaactgg tggccaactt tgccgatatc
    12001 tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg acgtcctgcc gatagaaaaa
    12061 cccgatcgca tcatcgccca tgttgacggc gtcccggttc tgaccagtca gtacggtact
    12121 ctcaacggtc agtatgcgtt acggatagaa catttgatta acccgatttt aaattctctg
    12181 aacgaggaac agcccaaatg agtgacatga ataatccggc cgatgacaac aacggcgcaa
    12241 tggacgatct gtgggctgaa gcgttgagcg agcaaaaatc aaccagcgaa aaaagcgctg
    12301 ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag cggaacgttg caggatatcg
    12361 acctgattat ggatattccg gtcaagctga ccgtcgagct gggccgtacg cggatgacca
    12421 tcaaagagct gttgcgtctg acgcaagggt cagtcgtggc gctggacggt ctggcgggcg
    12481 aaccgctgga tattctgatc aacggttatt taatcgccca gggcgaagtg gtggttgttg
    12541 ccgataaata tggcgtgcgg atcaccgata tcattacccc gtccgagcgg atgcgccgcc
    12601 tgagccgtta gtgatgaata accatgctac tgtgcaatct tccgcgccgg tttctgctga
    12661 gccactgctg caggtgagcg gcgcactcat cgccattatt gccctgatcc tcgctgctgc
    12721 ctggctggta aaacggctgg gatttgcccc taaacgcact ggcgttaacg gtctgaaaat
    12781 tagcgccagt gcttcgctgg gcgcgcgtga acgggtcgtg gtggtcgatg tggaagatgc
    12841 acggctggtg ctcggcgtta ccgcagggca aatcaatctg ctgcataaac ttcccccttc
    12901 agcaccaacg gaagagatac cgcagaccga ttttcagtcg gtcatgaaaa atttgcttaa
    12961 gcgtggcggg agatcctgat gcgtcgtttg ttttctgtcg caccggtcct tctctggctg
    13021 attacgcccc tcgccttcgc gcaactgccg ggtatcacca gccagccgct acctggcggg
    13081 tatcaccagc cagccgctac ctggcggtgg acaaagctgg tcgctcccgg tgcagacgct
    13141 ggtgttcatc acctcgttga cgttcattcc agcgatttta ctgatgatga ccagtttcac
    13201 ccgcatcatt attgtttttg gtttattgcg taacgcgctg ggaacgccct ccgcgccacc
    13261 taaccaggta ttgctggggc tggcactgtt tttgaccttt tttattatgt cgccagtgat
    13321 cgacaaaatt tatgtcgatg cgtaccagcc attcagcgaa gagaaaatct caatgcagga
    13381 ggcgctggaa aaaggggcgc agccgctgcg tgagtttatg ctgcgtcaga cccgcgaggc
    13441 agatttaggg ttgtttgcca gactggcgaa taccggcccg ttgcaggggc ctgaagctgt
    13501 gccgatgcgc attttgctcc cggcctacgt gaccagcgag ttgaaaaccg catttcagat
    13561 aggcttcacg attttcatcc cttttttgat tatcgacctg gtgattgcca gcgtgttgat
    13621 ggcattgggg atgatgatgg ttccccccgc caccattgct ctgcccttta aactgatgct
    13681 gtttgtactg gtggatggct ggcaattgct ggtcggttcg ctggcgcaga gcttttacag
    13741 ctagagaggc aaaatgacac ctgaatcggt catgatgatg ggaactgaag cgatgaaagt
    13801 cgcgctggca ctggcagccc cgctgttgct ggtagcgctg gtcacgggcc ttatcatcag
    13861 tattttgcag gcagccacgc agattaacga aatgacgctg tcgtttattc cgaaaatcat
    13921 cgccgtattt atcgccatca ttattgccgg accgtggatg ctcaatctgt tgctggatta
    13981 cgtccgcacc ttgttcacta acctgccgta tatcatcggg tagcccgtac aatgatgcag
    14041 gagacaagcg atcaatggct ttcctggtta agcctgtact tctggccgtt actgcgcgtg
    14101 ctggcgctca tttctaccgc gccaattctg agcgaacgca gcgtgccgaa acgggtaaaa
    14161 ctgggtctgg caatgatgat cacgttcgcc attgccccat cattacctgc caacgatgtt
    14221 cctgtttttt cgttctttgc tctgtggctg gccgtacagc agatcctgat cggcattgcg
    14281 cttggtttta ccatgcaatt tgcctttgcc gctgtgcgaa ccgctggcga aattatcggt
    14341 ctgcaaatgg ggctgtcatt tgcgacattt gtcgatcctg gcagccatct caatatgccc
    14401 gttttagcgc gcatcatgga tatgctggca ttactgctgt tcctgacatt taacggtcat
    14461 ttatggttga tttcactgct ggtcgatacc tttcacaccc tgccgattgg tagcgaaccg
    14521 ttgaacagca atgcgtttct ggcacccacc aaagcaggga gtttgatttt ccttaacggg
    14581 ctgatgctgg cgttaccgct cattactctg ttgctgaccc tgaatctggc attaggttta
    14641 ctgaatcgta tggccccgca attatccatt tttgttattg gatttccatt aactctgact
    14701 gtcggcatct ctttaatggc ggcattaatg ccgttaattg cgcctttttg cgaacattta
    14761 ttcagtgaaa tttttaattt gctggctgat attattagtg aattaccatt aatataattc
    14821 cgtaacgttt atcatgctac cctaaggatt atccgaaaaa taatatctgc gaacatcttc
    14881 caggatactc ctgtagcgaa atatttgttt taagctcact cacatatcgc aacatttact
    14941 ttaagacaat tccaggcaaa ttatacaaca ctttacggga tagtaagtcc gcctgaaaaa
    15001 ttgcgagagt ggcgcattag gtgacccatg ttgttccgtt tagtcatgat gaaatattca
    15061 ggtaagggga attatcgtta cgcattgagt gagggtatgc catgtcaacg attattatgg
    15121 atttatgtag ttacacccga ctaggtttaa ccgggtatct gttgagtaga ggggttaaaa
    15181 aaagagaaat caacgacatt gaaaccgttg atgaccttgc catagcttgt gattcacagc
    15241 gtccttcagt ggtgtttatt aatgaggact gtttcatcca cgatgcttct aacagtcagc
    15301 gcatcaagca catcattaat caacatccca atacgttatt tatcgttttt atggcaattg
    15361 ccaatgttca ttttgatgaa tatctattgg tcagaaaaaa atttattgat cagttctaaa
    15421 tcgattaaac cggaatctct cgacgatatc cttggcgata ttctgaaaaa agagacaacg
    15481 ataacctcgt ttttaaatat gccgacgtta tcattgagcc gaaccgaatc gagtatgttg
    15541 cgaatgtgga tggcaggtca gggaaccatt caaatctccg accaaatgaa tatcaaagcc
    15601 aagaccgttt catcgcataa aggtaatatt aaacgtaaga tcaaaacgca taataaacag
    15661 gttatctacc atgtcgtccg actgacggat aatgtgacta atggtatttt tgtcaacatg
    15721 cgctaacaca ttctgactgg tggtttccca ccagtcaggc tgaataagat tactctgctt
    15781 tctcaacaaa gataccgtcc tgatgccctg cttcattaaa gaaccagatg ccgagcgggt
    15841 agtcttccag cgcaaccagg tacattgtgc cttcactaaa ctcctcaact gccagtacca
    15901 ctccaggacg acgcggaccg ccatccgttt tgactgttac ccgatcattc accttcatcg
    15961 ttttcctcct gtggctttgt gccagtgtag aacaatttcg tttttctggc agcgccaagc
    16021 gcgcgcgagt gctgattttc tcgacggtct atacttaaga gatgccaggc ggacttaacg
    16081 actggcggca acaacagagt aacggttgcg aggaaagatg atgaaaaccg caaaagtgta
    16141 cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat gccctgctgg cggcgatcaa
    16201 tgaaattagc gaaagcgaag ttcattgatc gccgccagaa cgattctgaa cacgttagcg
    16261 tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa ctggatattc
    16321 atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc ccgaccctga
    16381 gggtgtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt acaccaggaa
    16441 atctgatgtg ttcatcacct tatctgcaat ttttttcgct gacaagaaaa tattcgcgct
    16501 atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct tggtggctct
    16561 ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt atttgtgctg ttttgagaat
    16621 catcctggta aggggagtac tgcgcgacca tgttttcaat tcaacaacca ctactggttt
    16681 ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa ccggcagccc
    16741 cctggctcag ccgtttacac gaagccaata ttcccgtcat tctctgtagc agtaaaactt
    16801 tagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg ctgattgcag
    16861 agaatggcgc agtgatccag cttgctgaac aatggcagga tatagacggt tttccacgca
    16921 tcatctcagg tattagccat ggcgaaatct gccaggtttt aaatacgcta cgcgagaaag
    16981 aacattttaa attcacgact tttgatgatg tcgacgatgc aaccattgcc gaatggacgg
    17041 gattaagccg tagctaggcg gcgctgacgc agctgcatga ggcgtcggta acgctaatct
    17101 ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg tctgaacgaa ctgggcttgc
    17161 agtttatgca gggtgcgcgc ttctggcacg tactggatgc ctctgccgga aaagatcagg
    17221 ctgccaactg gattatcgcg acctatcaac aattgtcagg cagacgccca accacacttg
    17281 gcctgggcga tgggccaaac gatgcgcctt tactggaggt aatggattac gcggtgattg
    17341 tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga ggatccggcc cgcgtctggc
    17401 gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt ttctccgccc
    17461 gttaagcgtt atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg aggtaaagcc
    17521 gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc tcgctgctac
    17581 ttacccccag cgaggcactg atgcgtatcg ttgtactctt ggcgatcagc atctcttttt
    17641 catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga ttcgcgcctg
    17701 gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc gcacgcaagg
    17761 aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga tgaccaaagc
    17821 ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaaa ggatgttggt
    17881 gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca cggttatata
    17941 aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga acatacatgt
    18001 tgctgaccat ccggcgaatc acataccagg agaggagtaa catggtggta aagagcgccc
    18061 acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct tcgcttagcg
    18121 tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg ttcccagcta acatagcgac
    18181 tgtccatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc aaggccagtt
    18241 cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc aaaaatctca
    18301 gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg tttcttaaaa
    18361 attgctgcat agtacgcacg ggaatactca tccccagcac gccataccag tagttattac
    18421 tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg
    18481 aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg ataaaccagg
    18541 gttgggtgac atagccgtaa taacgcgttg gtacattgcg cgtaaacaag gtcggctgcg
    18601 tcgaaacgta aaatccagca cgcgagacat acatcgcctg ttcaaccatc gacgaggagt
    18661 tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc
    18721 tttcattttc gcgagacagg aaattcccct cgctgactaa tgcatccgat acgccattga
    18781 ctggcagggt gcgccgtcgg ttgagttcga tttgccaggc gtgctcgtcg cgatgctgtt
    18841 cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgccacg agagcttcgc
    18901 gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg
    18961 cgttggctac gttttccaga tgattacgct agctggagat ataggcatct tccagcacca
    19021 ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag cagagattaa
    19081 cgacatgacc gggccccagg cggcgtgcga gttttttcaa ccagctctgg ttttccattt
    19141 ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct ggcataaccc
    19201 ggcaatagcg taccgcaaaa cagcgataat tttataatgg tggcatgata agacgcgact
    19261 atcattcatt gcaggcattg ggacaccagg ccggatgtga tccggcctgg tgttaatgat
    19321 accctttcaa acttcttccg cagaacgact cccctccccc ggaacaagat cggtttcatg
    19381 aaaagcttcc cgcttgaccc cgtaaccgtc ataccaacga cactcaacca taccgctgga
    19441 gtatccagtg acaatcatcc gcgggccgcc ctctttaacc gtaacttcct cactaaccat
    19501 aaagctcata ctcgcctcct tttttcgagt gaaacttgtt caccttagtt gaagatggcg
    19561 aattttgcat aatagccagt gcgagtatta atgtgcctga ccgcgcattt tcgctactgc
    19621 tttcactccc agcaccacga tgccgccgat gataaatcca agaatcagat ttaaaacagt
    19681 cggtaatatc atcgccacca ctgcactttg ctgcccggcg atatgttcaa tagcgtgatg
    19741 cagcggcgca ataccatgta ccacaatccc gccgccgacg aggaacatcg ccagcgtgcc
    19801 gacaatcgat aatgctttca tcagccaagg cgcgataatc aataaccctt tacctaatgc
    19861 ctgcatcagc gcgctagatt tttccgccag ccaataccct aggtcatcaa tcttaacgat
    19921 aacccctacc agaccgtaga cgcccacggt cactaccagc gcgatgcctg aaagcaccag
    19981 cacctgatta agcaacggcg cctctgccac aatccccagc gtgatggcga cgatttccgc
    20041 agacaaaata aaatcggtac gaatcgcccc tttaattttg tccttttcaa acttcagcgg
    20101 atcctgcgcc gccagcttct ccagacgctg ctggctctgc gctggatctt ctttatgttt
    20161 acgcgcctcc agcatatgca gcactttctc taccccttca aagcagagaa acgcgccacc
    20221 aatcatcaac agtggcgtaa tcgcccacgg gataaacgca ctgatgatca gcgccagcgg
    20281 caccagaatc actttattga tcagcgatcc tttcgccacg ccccagacca cgggaagttc
    20341 ccggttggcc cgcacgcctg aaacttgttg cgcattgagc gataaatcat cccctaatac
    20401 accggcggtt ttcttcgccg ccagtttgcc catcacagag atatcgtcca gcagtgtggc
    20461 gatatcatcg agcaacgtca gtaaactact cccggccaaa agcattccct ctcattttta
    20521 ttgttgaagg gtgtaagtat gaagcaaaaa cggcagctct gatacttgct tcacaggtca
    20581 acatttattt aacaatcaag tgcaattata tctctcgcca gcagaatgat tttcacgttt
    20641 actatgaacc acctttttat ttccgccgtg agggattatg cgtttccgcc agttgttacc
    20701 gctttttggc gcgctgtttg cgttgtatat catttggggc tcaacctatt ttgtcattcg
    20761 gattggcgtg ggaagctggc ctccgttaat gatggcgggc gttcgattcc aggcagccgg
    20821 tattttattg ctggcatttt tgctactgcg cggacacaaa cttcccccgc tacgtccgct
    20881 gctcaatgcc gcgctgattg gcctgttatt gctggctgtc ggtaatggca tggtgacggt
    20941 tgccgaacat caaaatgttc cttccggcat cgccgccgta gtgattgcaa ccgtgcccct
    21001 ctttaccctg tgcttcagtc gcctgtttgg cattaaaacg cgcaaactgg aatgggtggg
    21061 tattgccatt gggcttgccg gaatcatcat gctcaatagc ggtggaaatt taagcggcaa
    21121 tccgtggggc gcgattctga ttttaatcgg ctcgattagc tgggcgtttg gctcagttta
    21181 tggctcgcgc attaccttac ctgtagggat gatggcgggt gcgattgaga tgctggcggc
    21241 aggcgtggtg ttaatgatcg catcgatgat tgcgggtgaa aaactgacgg cgctcccttc
    21301 cctgtcaggc ttccttgcgg tcggctatct ggcgctgttt ggttcgatta tcgccattaa
    21361 cgcttatatg tatttaatcc gtaatgtcag tccggctctc gccaccagct acgcctacgt
    21421 taacccggtg gtcgcggtct tgctgggtac gggactgggt ggcgaaacgc tgtcgaagat
    21481 tgaatggctg gcgctcggcg taattgtttt cgcggtggta ctggtcacgt tgggaaaata
    21541 tctcttcccg gccaaacccg ttgtcgcgcc agttattcag gatgcatcaa gcgagtaaat
    21601 gaatcccctg cgtgtcgatc tgcgcgctgg caccttcgcc gcagatccac tcttccagac
    21661 gctcggtaag cgccgcatcc gtcagcttct cgcgcctacg taacgcacac tcccagacaa
    21721 tcagtacgcg ccagccgagc tcctgcaagc gactgatatc gcggcgatcg cgttcaacat
    21781 ttttacctat cttctccagc cagaactcag ttcgagtcgc aggcacttta aacagatagc
    21841 agtgatgatg atgccagaag cagccgtggg taaatatcac gcagcgatat tcatcaacga
    21901 caaaatccgg gcgtccgggc agactggcgt cctgaacgcg aaacgcaagg ccctgcccgg
    21961 ttaacaaact ggcgaggcgc ttctcaatcg ccgtatcacg cgtggcaatc gcgcgcatat
    22021 ttttgctgcg ggtggcctta tcgtgaacgt cgaccatgtt gtgcctcttg ctgacgcaac
    22081 gccaccgcct gtttgatttt tggctcaagc agttttgcca ccgcggcaaa gaccggcacg
    22141 accaccgagt tgccgaactg gcgataggcc tgagtgtccg aaaccggaat gcggaatttc
    22201 gcttctcccg gcgcttcaaa acccattaag cgcgcgcatt cacgaggcgt taaccgacgt
    22261 ggacgatgtt gctgattcag cggatcgtca aagtctttct cacccgtggc catatcccaa
    22321 ccgcgatcga ttaaaatttc cgcgccatct ttgtaataac gagcagacag cgtacgggtg
    22381 acgctttgcg gattgttcgg ataaaccatt ccataaccga agccgttacc gcgcgcctga
    22441 tgttttttcg catatcgata gaggtacttc cacagcaccg gcgtcaggat atatttcgcc
    22501 tcaaccatcg ggtccagcag ctgcgccagc gtcactcgct gtgcagggaa acattcgctg
    22561 atatcacgca gagtaaaatc ggctttcaga ttcagatcgc gacgaaaacc caccagcacg
    22621 atgcgttcac ggtgctgcgg caggaaatgt ttaccatcga tgattttcgg atcgtccggc
    22681 ccgttatctt ctgcatcggc aacgtcatag cccagttcgt ccagcgtctg catgatgatg
    22741 cggaacgttt ttcccttatc gtgactcttc aggtttttga cgttttcgag cacaaacatc
    22801 gccggacgac gcgcgtcgat aatgcgcacc acatcaaaga acagcgtgcc ctgggtatcg
    22861 caggcaaaac cgtgcgcccg cccgagcgag ttctttttcg atacgccagc cagcgaaaat
    22921 ggctgacaag ggaaaccggc cagtaaaaca tcgtgttcag gaatgtgttg acgaatatgt
    22981 tccgccgccg cctcatcact cacgccttct tgatgactga gggtgatgtc gcggatatct
    23041 tcattaaaat gatgcgtcgc cgggtcgcaa taatggttgg ctttataagt gcgtaccgca
    23101 tgtttgttcc attcgctggt aaacacgcac tgtccaccaa tcgattcaaa accacgacgg
    23161 atgccgccaa ttccggcaaa tagatcgata aagcgaaacg cataatgcgg atgatgttcc
    23221 ggtggtttgg gtaacaacgt ttgcagatgg gcaaactctt tctcacttaa acggtgccat
    23281 gccgagtcat tcgccagcgc acgttttaaa attgccgcgc tccagtgatt ctcacccaca
    23341 ccattaagct gcgccaccag cattttaaca tcataaattt gcagcagttt ctccagcatt
    23401 gcctgtgcgg cattcccggt gctgtatgaa tcggttactg atatattttc ctgcatagat
    23461 ttaaccggcc atctgagatg gccgacaggt taacataatt actgccgttt agggccacgc
    23521 gctcagccag cgggtgaaaa cgcatctatc accttgtgat cgataccttc attctccccc
    23581 gccagttcgg caccgagctt cgccatcaaa cagcgccaag ctcaatgccc ctgaaacggc
    23641 aaatacgcgc gccaggccaa tcgcccccag ggcttccagc cgatccgcat cctggacgat
    23701 tttagcttcc gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg caatggcatg
    23761 acaaacggcc tcaattttct ccgccggaaa ttgcacgaac tcttcacgga gcaggcgacg
    23821 cgtctcttct gccgccagga ttgaagaacg ctgccgttgc ggatgatttt tcgccaggct
    23881 cacgatatcg tgaaaataac aggcggttaa aatcaccagc atatcgacat cgtcatctgc
    23941 cgccagtttc tgtgccgtag cccagacacg gcgaaaatga cacaggtcat gcgccgcgtc
    24001 ctgatgttga tgatggtttt tcaaccagtt ctcgaactat gcctgccagt gttgtaactc
    24061 catacgcgct ccgttgccaa agagtctcaa ccttagcagt ttttattcat agcgtctgca
    24121 acgtgcggaa cgaggtcggg tgtaccatgc aatgccgcct aacaaggcac caaccagggc
    24181 cataaacaga aatccgacca atgctcctaa ccacttcccc gcggtagatc ctaaatcact
    24241 ttcaaatccc gacacggggg catcggggat ccggctcatc acccagatgt atcgcgccat
    24301 tgcccagacg aaatagccac accatgtata gaaagccc
//
LOCUS       DAZPEU010000044        24314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPEU010000044 DAZPEU010000000
VERSION     DAZPEU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.044"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            139..1638
                     /gene="pitB"
                     /locus_tag="ACHU2L_001601"
     CDS             139..1638
                     /gene="pitB"
                     /locus_tag="ACHU2L_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR1356503.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1687..2379)
                     /locus_tag="ACHU2L_001602"
     CDS             complement(1687..2379)
                     /locus_tag="ACHU2L_001602"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1356504.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPDAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2553..3266
                     /locus_tag="ACHU2L_001603"
     CDS             2553..3266
                     /locus_tag="ACHU2L_001603"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1356505.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3298..4056
                     /locus_tag="ACHU2L_001604"
     CDS             3298..4056
                     /locus_tag="ACHU2L_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356506.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            4102..5451
                     /locus_tag="ACHU2L_001605"
     CDS             4102..5451
                     /locus_tag="ACHU2L_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR1356507.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5451..6275
                     /locus_tag="ACHU2L_001606"
     CDS             5451..6275
                     /locus_tag="ACHU2L_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356508.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            6287..6847
                     /locus_tag="ACHU2L_001607"
     CDS             6287..6847
                     /locus_tag="ACHU2L_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1356509.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6878..7948
                     /gene="lptF"
                     /locus_tag="ACHU2L_001608"
     CDS             6878..7948
                     /gene="lptF"
                     /locus_tag="ACHU2L_001608"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1356510.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            7945..9024
                     /gene="lptG"
                     /locus_tag="ACHU2L_001609"
     CDS             7945..9024
                     /gene="lptG"
                     /locus_tag="ACHU2L_001609"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1356511.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9060..10231)
                     /locus_tag="ACHU2L_001610"
                     /pseudo
     CDS             complement(9060..10231)
                     /locus_tag="ACHU2L_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10231..10479)
                     /locus_tag="ACHU2L_001611"
     CDS             complement(10231..10479)
                     /locus_tag="ACHU2L_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1356512.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10512..11426)
                     /locus_tag="ACHU2L_001612"
     CDS             complement(10512..11426)
                     /locus_tag="ACHU2L_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1356513.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11423..13111)
                     /locus_tag="ACHU2L_001613"
     CDS             complement(11423..13111)
                     /locus_tag="ACHU2L_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR1356514.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            13515..14656
                     /gene="yghO"
                     /locus_tag="ACHU2L_001614"
                     /pseudo
     CDS             13515..14656
                     /gene="yghO"
                     /locus_tag="ACHU2L_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(14780..15550)
                     /gene="glcC"
                     /locus_tag="ACHU2L_001615"
     CDS             complement(14780..15550)
                     /gene="glcC"
                     /locus_tag="ACHU2L_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR1356515.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            15801..17300
                     /gene="glcD"
                     /locus_tag="ACHU2L_001616"
                     /pseudo
     CDS             15801..17300
                     /gene="glcD"
                     /locus_tag="ACHU2L_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            17300..18352
                     /gene="glcE"
                     /locus_tag="ACHU2L_001617"
     CDS             17300..18352
                     /gene="glcE"
                     /locus_tag="ACHU2L_001617"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR1356516.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            18363..19582
                     /gene="glcF"
                     /locus_tag="ACHU2L_001618"
                     /pseudo
     CDS             18363..19582
                     /gene="glcF"
                     /locus_tag="ACHU2L_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            19587..19991
                     /locus_tag="ACHU2L_001619"
     CDS             19587..19991
                     /locus_tag="ACHU2L_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFR1356517.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            20013..22184
                     /gene="glcB"
                     /locus_tag="ACHU2L_001620"
     CDS             20013..22184
                     /gene="glcB"
                     /locus_tag="ACHU2L_001620"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR1356518.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFFRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            22539..24125
                     /locus_tag="ACHU2L_001621"
     CDS             22539..24125
                     /locus_tag="ACHU2L_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR1356519.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         5778 a         5985 c         6526 g         6025 t
ORIGIN      
        1 ttgaatgaat gcttattgtc tgatacacaa gaaataacac tctttttatc gttaaaaaat
       61 gatatttcac tttgcccatg ccgttaaagt cactgataat gcgtccgttc gtaaattcaa
      121 aatggcgtaa tctaatatat gctaaattta tttgttggcc ttgatatata tacggggctt
      181 ttgttattac ttgctctggc atttgtgttg ttctacgaag caatcaatgg ttttcatgac
      241 acggcgaatg cggtggcaac cgttatttat actcgtgcca tgcagccaca acttgctgtg
      301 gtgatggcgg cattttttaa cttttttggc gtgttattgg gcggacttag cgttgcctat
      361 gccattgtcc atatgttacc aatcgatttg ttgctgaata tggggtcaac tcacggtttg
      421 gcgatggtct tttccatgct gctggcggcg attatctgga acctgggaac gtggttcttt
      481 ggtttaccgg cctccagttc gcacaccttg attggtgcga ttatcggcat cggtttaacc
      541 aacgcgctgt taaccggctc atcggtgatg gatgcgttaa acctgcgtga agtaaccaaa
      601 attttctcct cgctgattgt ttcccctatc gtcggcctgg tcattgcggg tggtctgatt
      661 ttcctgctgc gccgtttctg gagtgggacg aaaaagcgtg accgtattca ccgcattccg
      721 gaagatcgca aaaagaaaaa gggcaaacgt aaaccgccat tctggacgcg tattgcgctg
      781 attgtttccg ctgcgggcgt ggcgttttcg cacggcgcga acgacgggca gaaagggatc
      841 ggcctggtaa tgctggtact ggtggggatt gcccctgctg gcttcgtcgt caatatgaat
      901 gcgtccggct atgaaattac ccgtacccgc gatgccgtta ccaacttcga acactacctg
      961 caacagcatc ctgaactgcc gcagaagttg attgcgatgg aacctccatt gcctgcagca
     1021 tcgactgatg gcacgcaagt aacagagttt cactgtcatc cggcaaatac ctttgatgcg
     1081 attgcgcgcg ttaaaacgat gctgccaggc aatatggaaa gttacgagcc gttaagcgtg
     1141 agtcagcgca gccagctgcg ccgcattatg ctgtgcatct ccgatacctc cgcgaagctg
     1201 gcgaaactgc caggcgtcag taaagaagac cagaacctgc tgaaaaaact gcgcagcgat
     1261 atgttaagca ccattgagta cgctccggtg tggatcatca tggcggtagc actggcgctc
     1321 ggcattggca ccatgattgg ctggcgtcgt gtagcgatga ccatcggtga gaagattggt
     1381 aagcgcggca tgacgtatgc gcaaggcatg gcggcacaaa tgacggcggc agtgtctatc
     1441 ggccttgcca gttatattgg gatgcctgtc tccacaacac acgtcctctc gtctgcggta
     1501 gcagggacga tggtggtgga cggcggtggg ttacagcgta aaacggtaac cagcatcctg
     1561 atggcgtggg tgtttacttt accggcggca atttttcttt ctggtgggct gtactggata
     1621 gcattgcagt tgatttaacc ttcctgaaaa tgcccggtcc tggcgatcgg gcatttccat
     1681 ttttgactag tgataaccac gcgcggtcat aaaatccgta atcgcttttt ctgcatcaac
     1741 caacacctgt tccagtggct gattggcatc gatatcaacc agttgtgcac caccaaaggt
     1801 taactgtggc gttatggcaa tcttcctcgc cagcgattcc cgtttatggt cgggtttacg
     1861 tgcacaggca acttcaaggt caacattgag tttgatgacc agatcaggct tatggctcgc
     1921 catccagtga aacgctttac gttcctggct tgccagccat gagacaaaac gaccaccttc
     1981 aacgttaggt gggaacaccg taccatcgta agcgtcagga atttggtcct gaggataacg
     2041 gtcggttaga acaattaacc cgcgacgacg acaggcaagc atatgacgaa agcgcagtaa
     2101 gcgacgggcg acaaacgctg taattaccag cgccggaact ggtccaggca atttttttgc
     2161 tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca aaggtaattt
     2221 tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt cggcagcacc
     2281 atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg aagcatcgct
     2341 accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct ttaatttaaa
     2401 tatccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat tcaggtcaat
     2461 gtcacaatta gtcattgtca caataaggtt gaacggatat tctgagcacg gaccacgtaa
     2521 tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg tcaataatta
     2581 attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt aaatcgaccc
     2641 tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac atttatctcg
     2701 ggcaatcgtc cgggcgaatt ggcgaatgga tttcacagct ccctgttatt ggcgcacctt
     2761 gtgggtgtta tctgcgaagt aaagcggcac atgtacacga aaagccctta actccgcctg
     2821 gcaatattac tgcgctggtt atctatctgc tttcctgctg gcgggcgtac aagtttcgca
     2881 aaatgttgtg taaaagccag caaggctatc tgctcatcac cgaccgctac ccgcaggttg
     2941 aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc ggtaacggtt
     3001 ggataaaaat gttaaggcag cgcgagttga agctgtacca atggatggcg tcttatttac
     3061 ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt aaacctgacc
     3121 accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgaca ttcaatggcg
     3181 ccaagatcct tgaactggat ggtcggcaac cagccgatga aattatgcaa gcgtcactac
     3241 gcgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat ttaacgaatg
     3301 gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccaa ctacattccg
     3361 gggctgattg cggtggtcgg gtgtgatggc accggtaaat ccacgctgac caccgacctg
     3421 gtgaaatcgc tgcaacaaca ctggcaaacc gagcgccgct atctggggct gctctccggc
     3481 gaagacggcg acaaaatcaa acgattgccg ttggttggcg tctggctgga acggcgattg
     3541 gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct atgggcggcg
     3601 gtgattatgt actgcttctc gctgcgaaga atggcgaatc tacgcaaggt tcagcgactg
     3661 gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat ttcgggcttt
     3721 tattatgacg gaccggggat cggcgtcgaa cgtgcgaccg ggaaaatcag catgtttctg
     3781 gcgcagcgcg aacggagttt ataccaaaaa atggcgcaat accgtcctga attaatcatt
     3841 cgtttgggga ttgatattga tactgccatc tcccgcaagc ctgaccatga gtatgccgag
     3901 ctgcaggaca aaatcggcgt catgtcgacg attggctata acggcacaaa aattcttgag
     3961 attgattcca gagcgcccta cagcgaagtg ctggaacagg tacagaaggc ggtttctctg
     4021 gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatat tttttactat
     4081 caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt tgcagatggc
     4141 gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt cgagagcgcc
     4201 ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc catgtttggt
     4261 gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa gtttcagaca
     4321 tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc gcagcaattt
     4381 cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt ggcgattgtc
     4441 ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga ccaaagcttt
     4501 tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac gccgaccggc
     4561 attctgcgtg cggtagatcg ctttgatttg attgctgtac agcaggcgac gaaacctttt
     4621 ctgcgcgcag cggggagcgt cgcagcctgg tattttgact ttggttttgt gggttttgtt
     4681 attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt tgccgcgcgc
     4741 gaattacgcc gtagaaatat ccataacgcc ttcaaattga atctgtttga gtctgcccga
     4801 cacattaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc catctggtcg
     4861 gcgcgtaact cgtgcagcac agtgttggtg gggatcgtgt taggacccgc tgccgccggg
     4921 ttatttaaaa tcgccatgac attcttcgac gccgccggaa cgccagcggg tttgctgggt
     4981 aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc gtggttgtta
     5041 ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct cgcggtgttg
     5101 atcgtcggca agccgctcat ttcgctggtg tttggcgtta agtatctcga agcgtatgac
     5161 ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggcttccc gcaggagtca
     5221 ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat cgcctcaatc
     5281 ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg agcagcgttt
     5341 gcttacttcg gtgggcaatg tctggacgtg gcgctctcgt taattccaac cttaaaggca
     5401 ttttttcagc gccattcctt gctttataac gcagccggag agaaatcctg atgatgagaa
     5461 aatatttccc gcttgaggcg agcgagcgtc tatttgttgc gattgaagaa gatgatgtcg
     5521 ttgatgcaca ggttagtttg ccgccgacta ttgcactaag ttgtaccacc gaaatcattc
     5581 atgataatta cgcgttatgc ctgcaattct ggctcaatgg cgttgatcgt caggaattac
     5641 tgcgtttagt tcgcaaacaa gccaaaggcg atgagctgac tgctgatgag cgcaaacaat
     5701 ttaaatacat gcgagcgcgc tacaagcacc tgcgttttgc gcaacggctg tatttaaaga
     5761 agcatcaggc cggattttta ttcggcaaaa cgaccgtttt tctggggcgt tttcaggacg
     5821 gctttcgtaa cggtaagaaa aatattgtgt cgtattacgg caacctgtta cggatttatt
     5881 taagctctcc ggtctggtcg ttggtgaact attcattacg ccatagccag ttagaaagcg
     5941 taagcagttt tattgcctat cggcaaaagc aaatgcatac cctgaaagag attattgcca
     6001 aaccgcgact aaccggcagg gaatttcatg atgtgcgaaa aatcatcagc cagcaggttt
     6061 cttattacga caccttaaga tcgcttgatc ctgaaaataa agaggcgtta cagatttccc
     6121 gtttcctcgc cgccattaat ggcctgatgg gagataaaca cgacgacatg gtggcggatg
     6181 atatggaaaa ccgccagtcg tattatgcac cgttggcgct ggatagcgat atttgtcagc
     6241 gcttagagct gctgatttcg cgttttccgc tatagccgga gcaatgatgt catctcgttt
     6301 gattattgcg ttgattatca tgctcttagc cccgggtgtg caggcacaca atttcgtcac
     6361 tggcaaaacg gttacccctg tttatatcca ggaaggcggc gagctgcttc tgaatagcga
     6421 cgacgaaatt cactaccaaa agtggaacag cacacaatta gcgggaaaag tccgcattat
     6481 tcagtatatc gccgggcgta agtcggcgaa gaaaaagaac tcactgttaa ttaaagcggt
     6541 tgaagcggca aactttccgc aggatcgttt tcagcccacc accattgtga acaccgatga
     6601 tgccatcttt ggtactggct attttgttgt tggcaaaatc gaaaaaaata aacgccgcta
     6661 tccgtgggcg caattcgtta ttgacggcaa tggacaggga cgcgttgcct ggcgcttgcc
     6721 ggagcagagt tcaaccattc tggtgctgaa taaagccggg caaattcagt gggcaaagga
     6781 cggcagcctg acgccggaag aggtagatca tgtcattgcg ttggcgcaga aactgatcaa
     6841 tgaataaaaa actcaaagga ttgagggcgt tagtgagatg aagctggttg aacactacat
     6901 catgcgtggt acgcgccgtc tggtgctgat tatcgtcggt tttctgatct ttattttcgc
     6961 cagctactcc gcacagcgtt atctgaccga agcggcaaat gggacattag ctcttgatgt
     7021 tgtgctggat atcgttttct acaaagtact gattgcactg gagatgttgt tacctgttgg
     7081 tctgtatgtg tcagttggcg tgacgctagg gcagatgtat accgactcgg aaattaccgc
     7141 tatctctgcg gcggggggca gtccgggacg cttgtacaaa gccgttcttt atctggcgat
     7201 accgctaagt atttttgtca cccttctgtc gatgtatggt cgtccgtggg cttatgcgca
     7261 gatttatcaa ctggagcagc agtcgcagtc ggagctggat gttcgccagt tgcgggcaaa
     7321 gaaatttaac actaacgata acggacgaat gatcctttcg cagacggttg atcaggataa
     7381 taatcgcctg actgacgcgc tgatttatac ttctactgcc aatcgaaccc gtattttccg
     7441 cgccggttcg gttgatgtgg ttgacccatc acccgagaaa ccgaccgtta tgttgcacaa
     7501 cggcactgcc tatcttctcg atcatcaggg gcgtgacgac aacgaacaga tctaccgtaa
     7561 tctgcaatta catctgaatc cgctggatca aagcccgaac gtcaaacgca aagcgaaatc
     7621 ggtcacggag ctggcgcgct ccgcctttcc tgccgatcat gccgaactgc aatggcgaca
     7681 aagccgtggc ctgaccgcat tgttgatggc gctgctggcc atttcattaa gtcgggtaaa
     7741 accgcggcaa gggcgatttt caacgttatt gccactgacg ttgctgttta ttgccatttt
     7801 ttatggcggc gacgtctgcc gtacactggt ggctaacggc gcgattcccc tcattcctgg
     7861 tttgtggtta gtgcccggac tcatgctaat gggcctgctg atactggtcg cacgtgactt
     7921 ctctttgctg cagaaatttt cccgatgaat gttttcagtc gctatttaat ccgtcatctc
     7981 tttctcggtt ttgccgccgc tgcagggcta ttgctgccgc tttttaccac cttcaacctg
     8041 attaacgaac tggatggtgt cagcccgggc ggttatcgct ggactcaggc ggtgctggtg
     8101 gtgctaatga ccttaccgcg cacactggtc gaactttcgc cgtttatcgc gttattgggt
     8161 ggtattgttg gactggggca gttatcgaaa aacagtgagc ttaccgccat tcgcagcacg
     8221 gggttttcta tcttccgtat tgcactggtg gcgctggttg ccgggatatt gtggactgtt
     8281 tcgttaggcg cgatagatga gtgggtggcg tcgccattgc agcaacaggc gttgcaaatc
     8341 aaatcgaccg ccaccgcgtt gggggaggac gatgacatta ccggcaatat gctgtgggcc
     8401 aggcgtggta atgaatttgt gacggtgaaa tcgctgaacg agcagggcca gccggtgggc
     8461 gtggagatat ttcattatcg cgacgatctc tcgcttgaat cctacattta tgcacgcagt
     8521 gccaccattg aagacgacaa aacgtgggtc ctgcatggtg tgaatcataa aaaatggctg
     8581 aatggcaaag aaacgctgga aacattagat aatcttgcct ggcaatcggc cttcaccagt
     8641 atgaatcttg aagagttatc aatgccgggg aatacttttt ctgtccgtca gcttaatcat
     8701 tacatccatt atttgcagga aaccgggcaa cccagcagcg aataccacct tgcactgtgg
     8761 gaaaaactgg ggcaacccat cctgaccctg gcgatgattt tgctggctgt gccgtttacc
     8821 tttagtgccc cgcgttcgcc tgggatgggt agccgtctcg ctgtaggcgt catcgtgggc
     8881 ttactcacct ggatcagcta tcaaatcatg gttaatctgg gattgctatt tgcgttaagc
     8941 gcacctgtta ccgcgctcgg tttaccggta gcgtttgtgt tggtggcgtt gagcctggtg
     9001 tattggtatg acagacaaca ttaaacccgc cgtggcgcaa acaccacggc gggctcacat
     9061 tactgcttca gagtagcaaa tgcctgaatg atggcatcga tttgctcacg actgtgggca
     9121 gcatttacgc tacagcgcag cagcgtgatg cctgcaggcg cggcaggcgg caggataaga
     9181 ttcacataca cgcccggcga gataagctcg cgccagatgc gtaaaccttc ctctttcgaa
     9241 ccgattatta ctggcactac cgggctaata tgtgaactta actcataacc cagtttttgc
     9301 agaccgtcat acagatggtt ggcgttatcc atcagctttt gccgcagctc tggttgtgtg
     9361 gcaatagttc ttaacgacga gcgcacggtc gcaatgcagg acggagaagg agaggcggta
     9421 aaaatatacg ggcggctgcc gtagcgcagc acttccatcg cttccgaaac gacagcaaaa
     9481 ccgccaatcg aggccagact tttactgaaa gtaccaacga taatatccac atcatcttca
     9541 acgccgacag cttcagccag tccgcgcccc gttgcgccta acacgccaaa cgaatgcgct
     9601 tcatcgacaa tcaaataacc accgagacga cgtttgatat caacaatttc cgccagcggc
     9661 gcaacgtcac cgagcatgct gtagatgccc tcaacgatga taatcgcctc tttcgcccgt
     9721 tcaccgaggc gaaccatgcg ccgttccaga tctttagcat cgttatggcg gaaacgaata
     9781 atttccgcgc caccgagaga acagacgtca taaatactgg cgtggctgtc ggcatccagt
     9841 aacaccacgg cgttatggtc ggcaagtgcg ctgatgacgc ctaaattagc ggtataaccg
     9901 gttgaaaaac gatagcggta gggcgattga aaaactcggc aatctctttc tctaacgcca
     9961 ggtgcggcgc ataactgccg tttgccatac gagaaccggt ggtgccggtg ccttgggccg
    10021 ctaacgcggc ctgaccttcg ctgatggcat catgattaaa ggttaaaccg agataattat
    10081 tggtcccggc cagaatcact tttttattgc cgatccggcc ttcggtggcg gaatacactt
    10141 catcaataca ggtgccaaac ggtgttaacc cgttgtcact aaattgcaga cgctcgccag
    10201 caaggcgcgc atatttatcg tatagcccca ttatttattc tccagccatg gaagtagagt
    10261 ttccattaat tgcgcagggg ttttgacatc cagcagaata ttaataggaa tggaaatatc
    10321 gaatctgtct tccagcatca ttaagagatc catcactttg atcgactcca ggccaagatc
    10381 gttgacgaga tcgctgtcgg gcttaatttc cacaccgttt tcgaccaaat cctgtaaaca
    10441 ggaaaggatg taatccatta ctatttcacg atttaccata gaaacgttaa cacaccttta
    10501 aaacaagaca atcaaaatag cccgttgcgt aatgcgtgtt ccaggctaat cccgggaaac
    10561 caattgatat cttcagaaat acggttatta cttgccgacc agtcggcgtg ggttaattca
    10621 cgaattttcg agcgggtcag cataggttct ttacctgcca ggcgactcaa cgcggtactg
    10681 atatccgcaa ggcctgtgag taccggcaga ggaatgtcaa ccattcgcac ggaaccacaa
    10741 cgggctttgg cggcaagctg tcgtacgcgt tgccagtcat agccaccggc gacgccatcg
    10801 cataattcat aggtttgcgt ctgtacagtt tcggcgctta accactgacc cactgcttgc
    10861 gcaaaatcgg tgacgtgaag aaaagagagt tgtgtatctg gcgcaccaag tcgtggcagc
    10921 aggccgcgta gcatccagtc aaacagcggt tttaactctt tatcgccggg accatacacg
    10981 gctgtcgggc gaaaaacgcc cagcgtaatt tcgtccgcca ttgctgccag tcgttgctcg
    11041 gcgacgtatt tggattttgc ataccaggag agctcgggat ggcgcgccgc cagcgaagag
    11101 ataaacagaa aacgttggca aaagccgctc tcttttgctg cttgcatcag gcgcaggctg
    11161 ccgtcaacgt tacagcgggt gaaaatctct tctttgtgcc cgcgcacttg cccggcgcaa
    11221 tggaccacta cgctggctcc ggcaaccagc tcgctaagtg aatgagtatc ttccagcgaa
    11281 ccgcgcaccc aggtgagatt atcattgacg tgagcgcggg cagtacgcgt caatgcgcga
    11341 acgtgaaagc cgcgggcgag caggttatcg ataatatatt taccgataaa cccggtagcg
    11401 cccgtcaccg cgacagtttg atttatgcca gggattcctg cacattaaga ctggcagcat
    11461 aagccttctg ataacgtttt ttcgcttccg cacgggcagg cttgccggag gacgttcggg
    11521 gaatactgtg gggcggcaac agatcgatag ccgcggtcac gccaaattcg ctttggatcc
    11581 gtgccgccag cgcgtggata agctgcccgc gacgttcttc gtcgctgatc cgacactgga
    11641 tctgcaaaat gattttttcc tgggcggtaa caaaagcaat cgcatcgcca gaatgaattt
    11701 ccggttcctg ttccgctata tattcaatat cctgcggcca gatattacgg ccacgaataa
    11761 taatcagatc tttaatgcgt ccggtgacat acagataacc gtccagcaga taaccgaggt
    11821 cgccggtgtc taaccagccc gtcgcggcaa tctcgtcttg cgaaacctgg tcgccaaagt
    11881 aaccgctcat cagactggga ccggagatgc aaatatggcc taccacacgt tccgcgaccg
    11941 gcatacctgc ttcattgcgg atttcaatac catgttccgg caacgctttg ccgcagttga
    12001 cgaaagtcga tacggcgcgt gtctctgcac ccggcgcgac ggctttaccc tgatattcga
    12061 ggatgtcgcg atccacttcg ttaaccacaa ccccggaggc ttcatcagag aagctgacag
    12121 ccagcgcatt ttctgccagt ccgtagcacg gcatgaaagt tttattgtca aagttaacct
    12181 gacggaaaca ttcagcgaat tgatggagtt gttctgcgga gatcggctca gcaccaatac
    12241 cagcgacgcg ccagcaggaa agatccagtt cagcgagatc tttttcattc acgcggcgtt
    12301 ggcacaattc atagccaaac ggcggcgcaa cggaaacggt gccgcgattt ttactgatca
    12361 atttaagcca ttgcaaagga cgcatggcaa aatcctgagt gcgcaaataa tctactgaaa
    12421 gttgcgtggc gacggggggc aggagaaagc cgaccagtcc catatcatgg tagaaaggct
    12481 gccaggagac gcagcggtcg ctagggcgta atttaatgcc atcgtggctt atagcacgta
    12541 gattagccat cacttcgcga tgggtgataa tgacgccacg gggaaaacgg gtgctgccgg
    12601 aggtgtactg gaggtaggcg atatcgttcg gaactggacg ctggagcgca acatcggctt
    12661 ccggtaatgc cttaaaccaa gcgtggctta aaacatgtaa ttcggggttg ttatgcgtcg
    12721 cggcattgac cagtggcaac cactcatcac cagtgataat ggctgcgggc tggcaacttg
    12781 ccagtaaacc ctggagtttg gcgctccagg aatcccgctg accgacgccc attggaatcg
    12841 ccaacgggac ggcgactaag ccagcatact gacaggcaaa aaaagcctct acaaacccgc
    12901 tactcgtttc ggcaatcagc gccacgcgtt cgcctttttt caggttcagc gataacaacc
    12961 gcttcgcacc agcttcggca cgcgttttta atgtctgata ttccagttga tcttcaagtt
    13021 ggcaacgtct gtcataaaaa ttcattccgg ctctactcag agcggcgtag tccaaagcat
    13081 caaccagcgt tggaaaatcg gcatagcgca taggtaggga atgcgtaaag attttattag
    13141 acatatacac cacgaaaaaa ttggatatca aaaataaaat gacaataatg cggtggatta
    13201 tataggcata aaagataaat gagatagtat tttcatcggt gtgaaaatga acattgattt
    13261 aaataatgag tttaagtttt ttgtttttat tatgaatgat atttaattta tgaatatatt
    13321 aagaagaagc cttgtccggt cgccttaaat tgacaaaagg cattgaggcg tttctgtgta
    13381 gcctctgacc ttatcataat cattaattaa tactatttat tcgtcgtttg tattcattga
    13441 gttgattatt aagatgccgc tcgttgctgt tgcaacctgt ttttcaccat taattatttg
    13501 ttatccacag agatatggaa tgcgatctgc tgatgattaa aattgaaaaa gtaattaata
    13561 aaaatgacct taaggcattt atcgcttttc cgtcatcact ttatcccgac gatccaaact
    13621 ggataccccc tttattcatt gaacgcaacg agcatttgtc tgcgaaaaat ccggggacgg
    13681 accatatcat ctggcaggcg tgggtggcaa aaaaagcggg gcagatagtt gggcgtatta
    13741 ccgcgcaaat agataccttg caccgtgagc gttacggtga agataccggt catttcggca
    13801 tgattgacgc cattgatgat ccgcaggatt ttgccgcgtt gtttggcgca gcggaagcgt
    13861 ggttgaagtc acaaggtgca agtaagatca gcgggccttt cagcctgaat atcaatcagg
    13921 aaagcggatt actgattgaa ggttttgaca caccaccctg tgcgatgatg ccacacggca
    13981 aaccgtggta tgccgcgcat attgaacaac tgggttatca caaaggcatt gatttactgg
    14041 cgtggtggat gcagcgaacc gatctcactt tctctccggc gctaaaaaaa ctgatggatc
    14101 aggtgcgcaa aaaggtgacc attcgctgca tcaatcgtca gcggtttgcc gaagagatgc
    14161 agatcctgcg tgagattttc aactctggct ggcagcacaa ctggggattt gtgccgttta
    14221 ccgaacatga attcgcgacc atgggcgacc aacttaaata tctggtgccg gacgatatga
    14281 tctacatcgc tgagattgat tctgcaccct gcgcgtttat tgtggcttgc ctaacatcaa
    14341 cgaggcgatt gccgatctga acggatcgct ctttcccttc ggctgggcaa aattgctgtg
    14401 gcgtttgaaa gtcagcggtg tgcgaactgc gcgagtaccg ctgatgggcg tgcgcgacga
    14461 gtatcagttc agccgcatcg gcccggtgat tgcgctgtta ttgattgaag ccttacgcga
    14521 tccgtttgcc cgccggaaga tcgatgcgct ggagatgtca tggatcctcg aaaccaatac
    14581 aggcatgaat aacatgctgg aacgcattgg ggcggagccg tacaagcgtt accgattgta
    14641 tgaaaagcaa atttaatacc agatcctgtt tagcgcaggg gcggtcattc gggagagggt
    14701 atagatgaac agatttaaca ccagatcctg ttaagtgcag gggcggtccc ctcgccttgg
    14761 ggatagggta aaactctgct cacattccca ccttgttcat ctccagcggc accgccgagc
    14821 gaatcaaatg gtggccttcc agctcgatat cgtggagatt ctttttcacg gtccgtacat
    14881 gatcgcgcgc tgcgcgctga gcgacgtgcg gcagccgctg caacaccgcg ttgtagatcc
    14941 gcgcatgctg gcgatcgatc tgcttttttt gctgtggtcg atggtagaga ttatttacgc
    15001 tggcaaacac tgaattaaac atcagatcgg tcaatgattg cagcgtaaac accagcacct
    15061 gattgtgaga agcctgacaa atggcgagat ggaaagcgtg atccaactgc gcatgttcga
    15121 tcagcgaaat ctctttgtgg ttctcactgg cggcgagcat tttttcataa cagcgggtta
    15181 tcacaacaaa atcagcctgc gttcccagcg ttgccgccag ccttgccgat tcgccctcca
    15241 gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg ctgcgtactg aacagatgga
    15301 tcagcgggct ggtgtcctgc tcccgattaa gccgtgcgac atgagaatcg cgaccctgcg
    15361 ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc ttcacgcagt gcggagcgtg
    15421 agaagccgag cttttcacac agtcgacgtt ccgagggaag cggctgaccg accttcagta
    15481 cgccgtcgat aattaaccgt tcgatactct ctgcaaccac ttcgcaaata gggcgacgtt
    15541 catctttcat tcccggacct cgtgcacagg taggaccaat ttttctgttc ttaactcgca
    15601 aaacacgcac atcacgcaag tgattatgtt acatcaattt aacattaagt taaccaagac
    15661 aaggtcacag agctggaaaa aaaatggtct gaccggtagc taaagagata gacgaaaacg
    15721 aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa aaatcagctg ccacacaaca
    15781 caacaaagcg aagcctactc atgagcatct tgtacgaaga gcgtcttgat ggcgctttac
    15841 ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga gcatgtccct ggacttgaga
    15901 tcctgcatac cgatgaggag atcattcctt acgagtgtga cgggttgagc gcgtatcgca
    15961 cgcgtccatt actggttgtt ctgcctaagc aaatggaaca ggtgacagcg attctggctg
    16021 tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc aggcaccggg ctttctggtg
    16081 gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc gcgctttaaa gagagcctcg
    16141 acattaaccc cgttggtcgc cgcgcgcgcg tgcagccggg cgtatgtaac ctggcgatct
    16201 cctaggccgt tgcgccgcat aatctctatt acgcgccgga tccttcctca caaatcgcct
    16261 gttcaattgg cggcaatgtg gcggaaaatg ccggtggcgt ccactgcctg aaatatggtc
    16321 tgaccgtaca taacctgctg aaaattgaag tgcaaacgct ggacggcgag gcgctgacgc
    16381 tgggatcgga cgcgctggat tcacctggtt ttgacctgct ggcgctgttc accggatcgg
    16441 aaggtatgct cggcgtgacc accgaagtga cggtaaaact gctgccgaag ccgcccgtgg
    16501 cgcgggttct gttagccagc tttgactcgg tagaaaaagc cggacttgcg gttggtgaca
    16561 tcatcgccaa tggcattatc cccggcgggc tggagatgat ggataacctg tcgatccgcg
    16621 cggcggaaga ttttattcat gccggttatc ccgtcgacgc cgaagcgatt ttgttatgcg
    16681 aactggacgg cgtggagtct gacgtacagg aagactgcga gcgggttaac tacatcttgt
    16741 tgaaagcggg cgcgactgac gtccgtttgg cacaggacga agcagagcgc gtacgtttct
    16801 gggccggtcg caaaaatgcg ttcccggcgg taggacgtat ctccccggat tactactgca
    16861 tggatggcac catcccgcgt cgcgccctgc ctggcgtact ggaaggcatt gcccgtttat
    16921 cgcagcaata tgatttacgt gttgccaacg tctttcatgc cggagatggc aacatgcacc
    16981 cgttaatcct tttcgatgcc aacgaacctg gtgaatttgc ccgcgcggaa gagctgggcg
    17041 ggaagatcct cgaactctgc gttgaagttg gcggcagcat cagtggggaa catggcatcg
    17101 ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag cgatgaaatc acgaccttcc
    17161 atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa ccctgggaaa aacattccca
    17221 cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca tcacggtcat ttaccttttc
    17281 ctgaactgga gcgcttttga tgctacgcga gtgtgattac agtcaggcgc tgctggagca
    17341 ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt cagggcagca atagcaaagc
    17401 ctttttaggt cgcccagtca ccgggcaaac gctggatgtg cgttgtcatc gcggcattgt
    17461 taattacgac ccgaccgagc tggtgataac cgctcgtgcc ggaacgccgc tggtggcgat
    17521 tgaagcggcg ctggagagcg ccgggcaaat gcttccctgc gagccgccgc attatggtga
    17581 agaagccacc tggggcggga tggtcgcctg cgggctggcg gggccgcgtc gcccgtggag
    17641 cggttcggtc cgcgattttg tcctcggcac gcgcatcatt accggcactg gaaaacatct
    17701 gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac gatctctcac ggttaatggc
    17761 cggaagctac ggttgtcttg gcgtgctcac tgaaatctca atgaaagtgt taccgcgacc
    17821 gcgtgcctct ctgagcctgc gtcgggaaat cagcctgcaa gaagccatga gtgaaatcgc
    17881 cgagtggcaa ctccagccat tacccattag tggcttatgt tacttcgaca atgcgttgtg
    17941 gatccgcctt gagggcggcg aaggatcggt aaaagcagcg cgtgaactgc tgggtggcga
    18001 agaggttgtg ggtcagttct ggcagcaatt gcgtgaacaa caactgccgt tcttctcgtt
    18061 accaggtacc ttatggcgca tttcattacc cagcgatgcg ccgatgatgg atttacccgg
    18121 cgagcaactg atcgactggg gcggggcgtt acgctggctg aaatcgacag ccgatgacaa
    18181 tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg acccgcttta gtgccggaga
    18241 tggtggcttt gccccgctat cggctccttt attccgctat caccagcagc ttaaacagca
    18301 gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac gcggaacttt gaggagcagg
    18361 ctatgcaaac ccgattaacc gaagagatgc ggcagaacgc gcgctggaag ccgacagcat
    18421 cctgtgcgcc tgtgttcact gcggattttg taccgcaacc tgcccaacct atcagcttct
    18481 gggcgatgaa ctggacgggc cgcgcgggcg catctatctg attaaacagg tgctggaagg
    18541 caacgaagtc acgcttaaaa cacaggagca tctcgatcgc tgcctcactt gccgcaattg
    18601 tgaaaccacc tgtccttctg gtgtgcgcta tcacaatttg ctggatatcg ggcgtgatat
    18661 tgtcgagcag aaagtgaaac gcccactgcc ggagcgaatg ctgcgcgaag gattgcgcca
    18721 ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag gtagggctgg tgctgcgacc
    18781 gtttttaccg gaacaggtca gagcaaaact gcctgctgaa acggtgaaag ctaaaccgcg
    18841 tccgccgctg cgccataagc gtcgggtttt aatgctggaa ggctgcgccc agcctacgct
    18901 ttcgcccaac accaacgcgg caactgcgcg agtgctggat cgtctgggga tcagcgtcat
    18961 gtcagctaac gaagcaggct gttgtggcgc ggtggactat catcttaatg cgcaggagaa
    19021 agggttggca cgggcgcgca ataatattga tgcctggtgg cccgcgattg aagcaggtgc
    19081 cgaggcaatt ttgcaaaccg ccagcggttg cggcgcgttc gtcaaagagt atgggcagat
    19141 gctgaaaaac gatgcgttat atgccgataa agcgcgtcag gtcagtgaac tggcggtcga
    19201 tttagtcgaa cttctgcgcg aggaaccgct ggaaaaactg gcaattcgcg gcgataaaaa
    19261 actggccttc cactgcccgt gtaccctaca acatgcgcaa aagctgaacg gcgaagtgga
    19321 aaaagtgtta cttcgtcttg gatttacctt aacggacgtt cccgacagcc atctgtgctg
    19381 cggttcagcg ggaacatatg cgttaacgca tcccgatctg gcacgccagc tgcgggataa
    19441 caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc gtcaccgcca acattggttg
    19501 ccagacgcat ctggcgagcg ccggtcgtac ctctgtgcgt cactggattg aaattgtaga
    19561 acaagccctt gaaaaggaat aaaaaaatga aaactaaagt cattcttagc cagcaaatgg
    19621 cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa aaataactgg tctgtttcca
    19681 ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag tcgcatggac gattgcgcgc
    19741 cgattgcggc ttacatctcc caggagaaag cgcgtaccgc cgcgctgggg cgtcgtgaaa
    19801 ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc gttcgtgact gcgccgttac
    19861 tgacgtcgct ggaaggcggc gtaccggttg ttgttgatgg gcaaattatt ggtgccgtgg
    19921 gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc gaaagtggca gcagcggtgt
    19981 tggcgaaata agcgaaaacg aggagagaaa caatgagtca aaccataacc cagggccgtt
    20041 tacgcattga cgccaatttt aaacgttttg tggatggaga agttttgccg ggcgtggaac
    20101 tggacgctgc tgcattctgg cacaatgttg atgagatcgt tcacgatctg gcaccagaaa
    20161 atcgtcagtt gctggcagaa cgcgatcgca ttcaggcggc gcttgatgag tggcatcgca
    20221 gcaatccggg gccggtaaaa gataaagcgg cctataaatc tttctttcgt gaactgggct
    20281 acctggtgcc gcaaccggag cgcgtgacgg tggaaaccac gggcattgac agcgaaatca
    20341 ccagtcaggc ggggccacag ctggtggttc cggcaatgaa cgcccgctac gcgctgaacg
    20401 cggcgaacgc tcgctggggc tcactgtacg atgcgttata cggcagcgac atcatcccgc
    20461 aggaaggggc gatggtcagt ggctacgatc cgcagcgcgg tgagcaggtt atcgcctggg
    20521 ttcgacgttt cctcgatgaa tcactaccgc tggaaaacgg cagctatcag gatgtggtgg
    20581 cgtttaaggt ggtcgataaa caattacgca tccagttgaa aaatggtaaa gaaaccacgt
    20641 tacgtactcc agcacagttt gtcggttacc gtggcgatgc cgctgcgccg acctgcattt
    20701 tgctgaaaaa taacggcctg catattgaac tgcaaattga tgccaacggg cggattggca
    20761 aagacgatcc ggcgcacatc aacgatgtta tcgtcgaagc tgctatcagt accattctcg
    20821 actgcgaaga ttcggtcgcg gcggttgatg cggaagataa aatcctgctg taccgcaatc
    20881 tgctgggcct gatgcagggg actctgcaag agaaaatgga gaaaaacggt cggcaaatcg
    20941 tacgtaaact gaatgacgat cgtcagtaca ccgccgccga tggctctgaa atttctctgc
    21001 acggacgctc gttgctgttt atccgcaacg tgggtcattt gatgaccatt cctgtgattt
    21061 gggacagcga aggcaatgaa atcccggaag gtattcttga tggcgtcatg actggcgcga
    21121 ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac tggcagcgtc tatattgtga
    21181 aaccgaaaat gcacggtccg caggaagtgg cgttcgccaa caaactgttt acccgcattg
    21241 agacaatgct cggtatggca ccgaataccc tgaaaatggg cattatggat gaagaacgcc
    21301 ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg caaccgcgtg gcgttcatca
    21361 ataccggttt cctcgaccgt accggcgatg aaatgcattc ggtgatggaa gctggcccga
    21421 tgctgcgtaa aaaccagatg aaatcgacgc cgtggatcaa agcctacgaa cgtaataacg
    21481 tgctttccgg tctgttctgt gggctgcgcg gtaaagcgca aatcggtaaa ggcatgtggg
    21541 caatgccgga cctgatggca gacatgtaca gccagaaggg cgaccaactg cgtgccgggg
    21601 caaacacagc ctgggttccg tcaccaaccg ctgctacgct ccatgcgctg cactaccacc
    21661 ataccaacgt acagagcgtg caagccaaca ttgcccagac cgagttcagt gctgaatttg
    21721 aaccgctgct ggacgatctg ctgactattc cggtggctga aaacgctaac tggtcggcgc
    21781 aagagatcca acaagagctg gataacaacg tgcaggggat tctggggtac gtggtgcgtt
    21841 gggtggagca ggggattggt tgttcaaaag tgccggatat tcacaacgtg gcgctgatgg
    21901 aagaccgcgc aacgctgcgt atctccagcc agcatatcgc caactggtta cgtcacggta
    21961 ttctgaccaa agaacaggtg caggcgtcgc tggagaatat ggcgaaagtg gttgatcagc
    22021 aaaatgctgg cgatccggct tatcgtccga tggcggggaa tttcgctaac tcgtgtgctt
    22081 ttaaagctgc cagcgattta atcttcctcg gcgtgaaaca gccaaacggt tataccgaac
    22141 cgttattaca cgcctggcgt ttacgcgaaa aagaaagtca ttaatagcgt caaaaaagcc
    22201 ccggcaagtt ttattgtcgg ggctatgata gtaactgcgg tagtacattt atcccggaaa
    22261 taaccggagc gattatcagg gtttaacaat attaaatacc cgtagcgcat tactagcggg
    22321 cctaaaacaa caatatggac atcttaacga tactctgcgc ctgcgtgtgc ggaacagaaa
    22381 cccttacccg atatacttgt tatgccagag gtagcaattg cgttagcggc gtactgttgc
    22441 tgtaaagtat aacgcctata ttttacagtt cagtcctctt ttgcttatta caaccttaag
    22501 tgtatcaagc atataaagat aataagagac tgaacaatat ggttacctgg acccaaatgt
    22561 atatgccgat gggaggactg gggctatccg ctctggtcgc cctgatcccg ataatattct
    22621 tcttcgttgc actcgcggta ttacgtctga aaggacatgt tgctggagca ataaccctta
    22681 tattatctat cctgattgcg atattcgcct ttaaaatgcc gattgatatg gcatttgctg
    22741 ctgcgggcta tggctttatt tatggattat ggccaattgc gtggattatt gtcgcggcgg
    22801 tgttcctgta taaattaacc gttgccagcg ggcagttcga tattatccgc agctcggtta
    22861 tctccatcac cgacgatcag cgtttgcagg tgttactgat tggtttctcc tttggtgcgt
    22921 tgctggaagg agctgctggc tttggtgcga cggtggcgtt tggtgcgttg ggcgtgccga
    22981 ttctggtcgc cggtcaggta acgggaatcg atccgttcca cattggcgca atggcgggac
    23041 gtcagttacc gttcctgtcg gttcttgtgc cgttctggct ggtagcaatg atggacggct
    23101 ggaaaggggt gaaagagacg tggccagcgg cgctggttgc tgggggaagc ttcgctgtca
    23161 ctcagttctt tacctctaac tatattggtc cggaactgcc ggatattact tcggcgctgg
    23221 tgagtatcgt ctcactcgct ttattcctta aagtctggcg gccgaaaaat accgaaacgg
    23281 caatcagcat ggggcaatcc gcaggtgcga tggtggtaaa taagccatct tctggcggtc
    23341 ccgtgccttc agaatatagt ctggggcaaa tcattcgtgc gtggtcaccg tttttaatct
    23401 taacggtgct ggtcaccatc tggaccatga agccgtttaa agcgttattt gctccgggcg
    23461 gcgcgtttta ttcactggtg attaatttcc agatccctca tttgcatcaa caagtgttga
    23521 aagcggcacc cattgtcgcc caaccaacgc caatggatgc ggtgtttaaa ttcgaccctc
    23581 tctcggctgg cggcaccgct atttttattg cggcgattat ctctatcttc atcctcggcg
    23641 tgggcatcaa gaaagggatt ggcgtctttg ccgaaatgct aattagtctg aagtggccga
    23701 tattgtcgat tggcatggtg ctggcattcg ccttcgtcac caactattcc ggcatgtcca
    23761 ccacgctggc gctggtactg gcaggtacag gcgtgatgtt cccgtttttc tcaccgtttc
    23821 tcggctggct gggcgtattc cttaccgggt cggacacttc ttcaaacgcc ctgtttggtt
    23881 cactgcaatc gaccacggcg cagcaaatca acgtctctga cactctgctg gtggcagcaa
    23941 ataccagcgg cggcgtaact ggcaagatga tctccccgca atctatcgcc gtggcctgcg
    24001 ccgcgacggg catggtgggc cgagaatctg aactgttccg ctacaccgtg aagcacagtc
    24061 tgatttttgc cagcgttatc accctgctgc aggcgtatgt gtttaccggg atgttagtct
    24121 cgtaaatcaa taaagccgga tgaaggtaaa tatcacccgg ctttccattt gtctgtcgtt
    24181 tatctcgcca gggaaccgtt tttacgattg gcttctttat acgtagtctc cgttttacga
    24241 tgcgaattgt ttaatattta tacaacttgg cttctgtgct ggttataaag tacccacttt
    24301 catgagatca attt
//
LOCUS       DAZPEU010000045        24181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPEU010000045 DAZPEU010000000
VERSION     DAZPEU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.045"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(22..594)
                     /locus_tag="ACHU2L_001622"
     CDS             complement(22..594)
                     /locus_tag="ACHU2L_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR1356520.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="ACHU2L_001623"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="ACHU2L_001623"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR1356521.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="ACHU2L_001624"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="ACHU2L_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR1356522.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="ACHU2L_001625"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="ACHU2L_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR1356523.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="ACHU2L_001626"
     CDS             complement(3556..5337)
                     /locus_tag="ACHU2L_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1356524.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="ACHU2L_001627"
     CDS             complement(5330..7102)
                     /locus_tag="ACHU2L_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1356525.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="ACHU2L_001628"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="ACHU2L_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR1356526.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="ACHU2L_001629"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="ACHU2L_001629"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR1356527.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10354
                     /locus_tag="ACHU2L_001630"
                     /pseudo
     CDS             8662..10354
                     /locus_tag="ACHU2L_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="ACHU2L_001631"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="ACHU2L_001631"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR1356528.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="ACHU2L_001632"
     CDS             complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="ACHU2L_001632"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR1356529.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="ACHU2L_001633"
     CDS             complement(11658..12029)
                     /locus_tag="ACHU2L_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR1356530.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="ACHU2L_001634"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="ACHU2L_001634"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1356531.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DVASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14533)
                     /gene="hupB"
                     /locus_tag="ACHU2L_001635"
     CDS             complement(14243..14533)
                     /gene="hupB"
                     /locus_tag="ACHU2L_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR1356532.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="ACHU2L_001636"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="ACHU2L_001636"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR1356533.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="ACHU2L_001637"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="ACHU2L_001637"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR1356534.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="ACHU2L_001638"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="ACHU2L_001638"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR1356535.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="ACHU2L_001639"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="ACHU2L_001639"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1356536.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="ACHU2L_001640"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="ACHU2L_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR1356537.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="ACHU2L_001641"
     CDS             21800..22378
                     /locus_tag="ACHU2L_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1356538.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="ACHU2L_001642"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="ACHU2L_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR1356539.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6664 c         5826 g         6024 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc acatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgccca ccaagactcc ttctgcactc cccttttgtt
    24181 a
//
LOCUS       DAZPEU010000046        24105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPEU010000046 DAZPEU010000000
VERSION     DAZPEU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.046"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(74..1270)
                     /gene="bcr"
                     /locus_tag="ACHU2L_001643"
     CDS             complement(74..1270)
                     /gene="bcr"
                     /locus_tag="ACHU2L_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR1356540.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVL
                     VWLSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVL
                     SYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVR
                     RIGALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVIL
                     DEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYA
                     SRPKKR"
     gene            complement(1298..1993)
                     /gene="rsuA"
                     /locus_tag="ACHU2L_001644"
     CDS             complement(1298..1993)
                     /gene="rsuA"
                     /locus_tag="ACHU2L_001644"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR1356541.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2143..3903
                     /locus_tag="ACHU2L_001645"
     CDS             2143..3903
                     /locus_tag="ACHU2L_001645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1356542.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            4028..4312
                     /gene="rplY"
                     /locus_tag="ACHU2L_001646"
     CDS             4028..4312
                     /gene="rplY"
                     /locus_tag="ACHU2L_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR1356543.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4451..5458)
                     /gene="yejK"
                     /locus_tag="ACHU2L_001647"
     CDS             complement(4451..5458)
                     /gene="yejK"
                     /locus_tag="ACHU2L_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR1356544.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5640..5867
                     /locus_tag="ACHU2L_001648"
     CDS             5640..5867
                     /locus_tag="ACHU2L_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR1356545.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            5887..7647
                     /gene="yejM"
                     /locus_tag="ACHU2L_001649"
     CDS             5887..7647
                     /gene="yejM"
                     /locus_tag="ACHU2L_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR1356546.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7722..7798
                     /locus_tag="ACHU2L_001650"
     tRNA            7722..7798
                     /locus_tag="ACHU2L_001650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7756..7758,aa:Pro,seq:ggg)
     gene            complement(7901..10492)
                     /gene="yejO"
                     /locus_tag="ACHU2L_001651"
     CDS             complement(7901..10492)
                     /gene="yejO"
                     /locus_tag="ACHU2L_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR1356547.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            10812..11459
                     /gene="narP"
                     /locus_tag="ACHU2L_001652"
     CDS             10812..11459
                     /gene="narP"
                     /locus_tag="ACHU2L_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR1356548.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(11670..12722)
                     /gene="ccmH"
                     /locus_tag="ACHU2L_001653"
     CDS             complement(11670..12722)
                     /gene="ccmH"
                     /locus_tag="ACHU2L_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR1356549.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12719..13276)
                     /gene="dsbE"
                     /locus_tag="ACHU2L_001654"
     CDS             complement(12719..13276)
                     /gene="dsbE"
                     /locus_tag="ACHU2L_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR1356550.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(13273..15216)
                     /gene="ccmF"
                     /locus_tag="ACHU2L_001655"
     CDS             complement(13273..15216)
                     /gene="ccmF"
                     /locus_tag="ACHU2L_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR1356551.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(15213..15692)
                     /gene="ccmE"
                     /locus_tag="ACHU2L_001656"
     CDS             complement(15213..15692)
                     /gene="ccmE"
                     /locus_tag="ACHU2L_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR1356552.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(15689..15898)
                     /gene="ccmD"
                     /locus_tag="ACHU2L_001657"
     CDS             complement(15689..15898)
                     /gene="ccmD"
                     /locus_tag="ACHU2L_001657"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR1356553.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(15895..16632)
                     /gene="ccmC"
                     /locus_tag="ACHU2L_001658"
     CDS             complement(15895..16632)
                     /gene="ccmC"
                     /locus_tag="ACHU2L_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR1356554.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(16674..17336)
                     /gene="ccmB"
                     /locus_tag="ACHU2L_001659"
     CDS             complement(16674..17336)
                     /gene="ccmB"
                     /locus_tag="ACHU2L_001659"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR1356555.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(17333..17956)
                     /gene="ccmA"
                     /locus_tag="ACHU2L_001660"
     CDS             complement(17333..17956)
                     /gene="ccmA"
                     /locus_tag="ACHU2L_001660"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR1356556.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(17969..18571)
                     /gene="napC"
                     /locus_tag="ACHU2L_001661"
     CDS             complement(17969..18571)
                     /gene="napC"
                     /locus_tag="ACHU2L_001661"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR1356557.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(18581..19030)
                     /gene="napB"
                     /locus_tag="ACHU2L_001662"
     CDS             complement(18581..19030)
                     /gene="napB"
                     /locus_tag="ACHU2L_001662"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR1356558.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(19027..19890)
                     /gene="napH"
                     /locus_tag="ACHU2L_001663"
     CDS             complement(19027..19890)
                     /gene="napH"
                     /locus_tag="ACHU2L_001663"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR1356559.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(19877..20572)
                     /gene="napG"
                     /locus_tag="ACHU2L_001664"
     CDS             complement(19877..20572)
                     /gene="napG"
                     /locus_tag="ACHU2L_001664"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR1356560.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(20579..23065)
                     /gene="napA"
                     /locus_tag="ACHU2L_001665"
     CDS             complement(20579..23065)
                     /gene="napA"
                     /locus_tag="ACHU2L_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR1356561.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(23062..23325)
                     /gene="napD"
                     /locus_tag="ACHU2L_001666"
     CDS             complement(23062..23325)
                     /gene="napD"
                     /locus_tag="ACHU2L_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR1356562.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(23315..23809)
                     /gene="napF"
                     /locus_tag="ACHU2L_001667"
     CDS             complement(23315..23809)
                     /gene="napF"
                     /locus_tag="ACHU2L_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR1356563.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            23918..24082
                     /gene="yojO"
                     /locus_tag="ACHU2L_001668"
     CDS             23918..24082
                     /gene="yojO"
                     /locus_tag="ACHU2L_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR1356564.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
BASE COUNT         5927 a         6828 c         5927 g         5423 t
ORIGIN      
        1 cggggaaatg atagatttgt ggttgacatg catcaacaaa ggaagccttt taccttcctc
       61 gttgtgcaat agatcaccgt tttttcggcc gactggcgta cagacagaag agaatggagc
      121 tggttgcgca gaatgcaatt gaccaaatca tcggccatgc agagttaaag gtcgcaagag
      181 aaagcaatgc gccaacaatt gcccctatgc caaaacggaa ggttcctgcc agcgaagatg
      241 ccgttcccgc catatgggga aactcatcaa gaatgaccgc catcgcattg gatgacacca
      301 tcgacacgca gcccacaaac gccgcaacgc caaccaccag cgaccagaat cccagcccca
      361 gcggcgcact gatgaccatc cacgcagcca taataaattg tatccacaac cccgagcgga
      421 acatatttaa cgcgccaatg cggcggacga agcggctgtt aaagatggtc atcacgaaca
      481 gaaaaacaat gtttagcgca aagtaataac caaagttttc cggcgcgacg tggttaattt
      541 caatataaac aaacggtccg gcgcttaaga atgagaacat cccggcaaag ctgaaaccac
      601 tggcaagcat gtagctcagg acacgtttat ggcggaacag cgccgcaaag ttaccaatag
      661 tggtacgaat gtgaaatggc tgacgacgct ccggtggtaa ggtttcttta atcaggaaga
      721 aaatcattgc cgaagccaga atcgccgcta atgccaggat ccagaagatg taatgccagc
      781 tcagccacac cagcacccag ccgccaacta tcggtgccat cagcagtgca atggtcgtca
      841 ccagcatgac aaacgacatc atccgcgaga actcttcttt cgggtaaata tcacgcatca
      901 aagcgttgat aaccacgctg gccgcagccg cagccgcagc cagcccgtgg aagaaacgca
      961 tcacaatcag ctgatcgatg gtttgtgcca acgcacacgc caccgcggcg gcggcaaaca
     1021 ccagcgtccc gccaagaacc accggcttac gcccgaagct gtctgccatc ggcccgtaga
     1081 ttaactgccc caacgcaaag cccagaatat aagtactgag ggtcatctgc gtactgcccg
     1141 ccgatacgcc aaactgcgct gaaattaccg gtagcgcggg cagatacata tcaatcgaca
     1201 gcggcatcaa catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc
     1261 gggtggtcac aacgggctcc tgaaagtcat tgaaaagtta gacgacgctg gcaatttctt
     1321 cttcagttaa cggacgatat tcaccggggg ctaaatcagc atccagcgta ataccgccaa
     1381 tacgttcacg atgcagctca accacgtggt tacccacggc ggcgaacatg cgtttcacct
     1441 gatgataacg cccttcgctg atggtcagac gaacctgcgt tggggtaatc acttcaagca
     1501 ccgccggctt agtgagatct ttttcgttat gcagctgcac gcctttagca aattgctctg
     1561 ccgtatcgtc agctacaggt gattccagtg tcaccagata ggtcttctcg caatgatggc
     1621 gcggagaagt aatgcggtgc gaccactgac catcatcagt catcagcacc agaccggtgg
     1681 tatcgatatc cagacgtcct gcggcgtgca gtttccacgc taccggttca tccagaaaat
     1741 agagcaccgt cgggtgatcc gggtcgtccg tggagcaaac atagccctga ggcttattga
     1801 gcatgaagta acgtggaccg tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat
     1861 gttcagggag cagtttgaac gctgcattac gaacgatttc gccatcgacg gtgacacgat
     1921 tgccgcggat ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt
     1981 tatcaagtcg catgtgtgtg attttgcctg taaaaaacgg aggtcgggta atgacccgaa
     2041 aatctgaact gttgtctgcc cagtatagcg gtctaataac ttccctcaag ggaaaaagaa
     2101 ccgtggcata ctattagcag aataatctac ctacgcgaaa ccatgatttt tacactccgc
     2161 ccatatcagc aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct
     2221 gccgttatcg tgctgcccac cggcgcaggt aaaagcctgg tgatagcgga actggcacgg
     2281 ctggcgcgtg gtcgcgtact ggtgctggca cacgttaaag aactggtggc gcaaaaccat
     2341 gcaaagtatc aggcgctggg gctggaagcc gatatttttg ccgccgggct aaagcgcaaa
     2401 gagagccacg gtaaagtggt atttggcagc gttcagtcgg tcgcccgtaa tcttgatgcc
     2461 tttcagggtg aattttcgct gttgattgtc gatgaatgcc accgtattgg tgacgatgaa
     2521 gagagccagt atcagcaaat cctcactcac ctgactaaag tgaatcccca cttacgcctg
     2581 ctggggctga ctgccacgcc ttttcgattg ggcaaaggct ggatctacca gttccattat
     2641 cacggcatgg tacgcggcga tgagaaagcc cttttccgtg actgcattta tgagctgccg
     2701 ctgcgttata tgattaaaca cggctatctg acgccgccag aacgactgga tatgccagta
     2761 gtgcaatacg atttcagccg cttgcaggca cagagtaacg ggctgttcag cgaagctgat
     2821 ctcaaccgtg agctgaaaaa acagcaacgc attacgccac atatcatcag ccagatcatg
     2881 gagttcgccg aaaagcgcaa aggggtaatg attttcgccg ccaccgttga acacgcaaaa
     2941 gagattgtgg gattactgcc tgccgaagat gctgcactga ttactggcga caaccccggc
     3001 gctgagcgcg atgtgttaat tgaagatttt aaagcccagc gttttcgcta tctggtcaac
     3061 gtcgcagtac tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc
     3121 cctaccgaat cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg tctcgctccg
     3181 ggcaagactg attgtttaat tcttgattat gcgggtaatc ctcacgatct ctacgcgccg
     3241 gaagttggta caccgaaagg caaaagtgac aacgttccgg tacaggtttt ctgccctgcc
     3301 tgcggttttg ccaacacctt ttgggggaaa acgaccgccg acgggacatt gattgaacac
     3361 tttggtcgcc gctgtcaggg atggtttgaa gatgacgacg gtcatcgcga acagtgtgac
     3421 ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcag aaaatgatat tgccgctcgc
     3481 cgctgccgcg aatgtgacac ggtactggtt gatccggatg atatgttaaa agcggcgcta
     3541 cgactgaaag atgcgctggt attacgctgt agcggcatgt ctttgcagca tgggcacgac
     3601 gagaaaggcg aatggttgaa aatcacctat tacgatgaag acggcgcgga tgtgagtgag
     3661 cgtttccgtc tgcaaacgcc cgcccagcgt accgccttcg agcagctttt tatccgcccg
     3721 catacgcgca caccgggcat ccctctgcgc tggatcaccg ccgccgatat cctcgcccag
     3781 caagccttac tgcgacatcc ggattttgtc gtcgcccgca tgaaaggtca gtactggcaa
     3841 gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga attacgcggt
     3901 taatggtagt tttgattgat gtataaggcg attgagtata gaatctcgcc cgcttttgca
     3961 tacgcaaagc agatcactta cctgttgctg ggtcgcctgt agcaggatta atttaagaga
     4021 gaaagaaatg tttactatca acgcagaagt acgtaaagag cagggtaagg gtgcgagccg
     4081 ccgcctgcgt gccgctaaca agttcccggc aatcatctac ggtggcaaag aagcaccgct
     4141 ggctatcgag ctggatcacg acaaagtcat gaacatgcaa gctaaagctg aattctacag
     4201 cgaagttcta accatcgttg ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca
     4261 gcgtcacccg tacaaaccga agctgcagca catcgacttc gttcgcgctt aattgctgaa
     4321 taagttgtaa aaaaccccgc tccggcgggg ttttttgtat ctgcagatta tgcctgatta
     4381 cggtattgct attttttgca ggccagataa ggcgttcacg ccgcatctgg cattcagaac
     4441 acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa ttggtcgcgc aaattcggcg
     4501 gcgtgccttt aatggtcaag gtatcggttg ctggatccca gaagatgcgt tcacctaaca
     4561 gcatcgcatc aaagttaata gtcaagccac cgccgctacc ggcaaacttc gtcaactgac
     4621 gcagtgtgct acggtctgcc gggaagctct cttccagttc gtagcctttt tcagccgcaa
     4681 actccgtgaa gctcacttcg cttacgcctg ccagctcttt cgacagcgat tccagttcga
     4741 tctcttcgcc tgcctgcagc tgctcattac agtagctata aacctgctgg cgcacgttct
     4801 gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc atcaaccgct tgcaacagcc
     4861 cacggttttg cgctttcgcg ttcaatcctt cgctggcccc gaggaaatcc ataaagaaat
     4921 ccgccacttt gcgccctact cgccctttca ggaaagtgag atagcgggtg gactctggat
     4981 tggtttccca ttcggttaaa tcgatacgcg caacaatatc cgcatgattg atatcaagat
     5041 aatgggttgg gttgatgtcg agattttcgt taacgcgcat actgctcagg ttgctcagaa
     5101 ctgccaccag caaatactcc accgccagat aacggtagtg gcaaaacagc acgaatccac
     5161 cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg accggttgcc gcgcggctaa
     5221 atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc agttcactct
     5281 cttcgctaaa caagccgtaa gctttatttt tggcgctata gacccgatgc agttctgcca
     5341 ccatctcaac gacggtttct gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt
     5401 tctgctcatc acgcttgata agctggtgca gagcaatctg gttgatatcc agactcatga
     5461 taaactctcc tttaagaccg ggcggtattc aaccaccgcc agttaagcga cgcaataaaa
     5521 gatggcggtc attttacaga aggcagcgta acgagaatga tttaacgcat aaaaaagcag
     5581 aaaaaaaacc gttgctacgg taatatgttg ccctttcatc aactaactga tttcgattta
     5641 tgccacaaat ttcccgctac agtaatgaac aggttgaaca attgctcgca gagctgctca
     5701 acgtactgga aaaacataag gctccgaccg acctttccct gatggtgtta ggcaacatgg
     5761 tcactaacct tatcaacacc agcattgccc cggcccaacg ccaggcaatt gccaactctt
     5821 ttgccagcgc cttacagtcc tctatcaacg aagacaaagc gcactaaggg aaacagataa
     5881 caggttatgg taactcatcg tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg
     5941 gggcactggt ttgcactgtt caatattctg ctttcgctcg tcattggcag ccgttacctg
     6001 tttatcgccg actggccgac aacgcttgct ggtcgcattt attcctacgt aagcattatc
     6061 ggccatttca gcttcctagt gttcgccacc tacttgctga tcctcttccc gctgaccttt
     6121 atcgtcggct cccagaggct gatgaggttt ttgtccgtca ttctggcgac ggcgggaatg
     6181 acgctattac tgatcgatag cgaagtcttt actcgtttcc atctccatct taatcccatc
     6241 gtctggcagt tggttatcaa cccagacgaa aatgagatgg cgcgcgactg gcagctgatg
     6301 ttcatcagcg tgccggttat tttattgctt gaactggtgt ttgcgacgtg gagctggcaa
     6361 aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc cgctggccgc attcttattt
     6421 atcgccttta tcgcctcgca tgtggtgtat atctgggccg atgccaactt ctatcgcccg
     6481 atcaccatgc agcgcgctaa cctgccgctt tcgtacccga tgacggcgcg acgtttcctt
     6541 gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc gtcttattga gcaaggtaat
     6601 ccagacgccg tttccgtaca gtatccgtta agcgaactgc gctatcgcga tatgggcacc
     6661 gggcagaatg tgctgttgat tactgtcgat ggcctgaact actcacgctt cgagaagcag
     6721 atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc
     6781 ggcaacacta cagacaacgg catctttggc ctgttctatg gcatctcgcc gagctatatg
     6841 gacggcattc tgtcgacccg tacgcctgcg gcattaatta ctgcgcttaa tcagcaaggc
     6901 tatcagctgg ggttattctc atcagatggc tttaccagcc cgctgtatcg ccaggcattg
     6961 ttgtcagatt tctcgatgcc gagcgtacgc acccaatctg acgatcagac cgccacgcag
     7021 tggatcaact ggctgggccg ctacgcacaa gaagataacc gctggttctc gtgggtttct
     7081 ttcaatggca ctaacattga cgacagcaat cagcaggcat ttgcacggaa atatagccgg
     7141 gcggcaggca atgtcgatga ccagatcaaa cgcgtgctca atgcactgcg tgattctggc
     7201 aaactggaca atacggtggt gattatcact gccggtcggg gtattccact gagcgaagag
     7261 gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc cattagtgat tcactggcca
     7321 ggcacgccgg cgcagcgtat taatgctctg actgatcata ccgatctgat gacgacgctg
     7381 atgcaacgcc tgctgcatgt cagcacacct gccagcgaat attcgcaagg tcaggatttg
     7441 ttcaaccctc aacgccgtca ttactgggtt actgctgcgg ataacgatac gctggcaatt
     7501 accaccccga aaaagacgct ggtgctgaac aataacggta aataccgcac ttacaactta
     7561 cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt tgttattgca agtgctgaca
     7621 gacgagaagc gttttatcgc taactgatta attataaatc agttagcgaa atatcttact
     7681 tgcaatcggt gtggaaaacg gtagtattag cagccacgag tcggcacgta gcgcagcctg
     7741 gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa
     7801 aatcccaaga aaaaaccaac ccttacggtt ggttttttta tatctgcaat taattcgata
     7861 aacagaccgt gacacatcac agcctgttta ttttctgtta tcagaacgtc cagaccacac
     7921 ccgcctgagt attccacggc gattctaccc ctgcgccatt gccatagctg actgacaaat
     7981 gaccgcttaa cgtcggggta aacgatgacc ttatcccagc ctgataaacg ccactggttc
     8041 cagccacatc attattaaat ttgccatcgt cattaacttt cacctggtta gaatcggcgt
     8101 attcctgacg cacggcggct ttcagccagg gttccagcgt cgtaccgttt tgcaggtcca
     8161 tgtgatagct taccgccgtt cccgcttcag cgcgtaatat ccgggtattt cccacatccg
     8221 cgcgcatgcc gtttgataac gtgtagtcct gaccatctgt ggtaaagccg gtaaaggcca
     8281 gatagggtct aacactccac aatccgtcaa cccaacggaa cccgctctca acatgagcac
     8341 ccgcgccgtt actattgtaa tcgccaaacg ctgttgcccc attactcatc ttgccatgga
     8401 tggtgttggc aaaacggtca actttcacca ccccatcaac ataggcaccg ttctgatgct
     8461 cccaaccggc ataagccccc agggtatagc tatcgatatt accttttccg ccgcgatcaa
     8521 aaccaatatc agaatgagag taaccaaaga acaagccgcg aattgtactg ctttcttcac
     8581 gggagaaacg gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca aaaccagctc
     8641 ccgcatcagt ggtcacgttg ttgcgggtgt taattgccga actccacatc accgtatcgt
     8701 aactaacgcc ttttacgcta ccaagacgct cacgcacggt gtccagttct gcatcaaata
     8761 ccagcggttg tgcggctgcc atgttcagca catcagtggt tgaaggggta atttgcgcgc
     8821 gattctctgc gaggctccag ctatggttgc cattatccag caaggtatat tcatacgtac
     8881 cgatatcaac aacgcctccg gcattgccca acgtaaatgc agcatcaccg ccgcccgttg
     8941 ttaccagtgt aaggctatct cctgctgccg ggctggcacc ggtgtccgtc acgaatattt
     9001 tgaaatcacc tgttgcctga ccggtgacgt tgagctgatc gctctgatga ttggccatat
     9061 cggtacgcat ataaaaattg ccgtttcccg acagggtatt ggtggtcaac gtaatgaaat
     9121 tttccgcact tgccgtcaca gatgaacgag tcactggctc agccatcata atcacatttg
     9181 cgccggacat cgcaaggttg gctaagttga ggcgagtgtt ttggttcgtc gtagtgatat
     9241 cggcaatgat ggtccatgcg ctattttcga gcgaaacgtc cgctttgctg gtatccacgg
     9301 caccatgcgt tctaaaagaa gcgccctcag aaaccactac cttacccacc aacatagcgg
     9361 gtgcataatt tatttgcggc ttcatgacct caagaatgcc atcattccct ctgactgaca
     9421 cgttaaccat tgtgccagcc cagacattag cgcggccatt gttgattacc atattttcag
     9481 agatggcttt tgaggaatac gtgatacttc cattctggtc tgatcgaccg agatcatata
     9541 ccgcatttgc ctgcactttc gtaccagtat cctttcccag cgattgcata gtggcatgct
     9601 catcaaggat agtgtcaatg gcctgcgtgt cctccataac aatgagcccg gaaccatcat
     9661 ccagttcata attttttgac acaccatcct ttatactaaa ttggccttta ctgtttgtac
     9721 cactcacttc cagcgcattc gttgagacaa ttaatttccc gcctgctttt ttatcaacgc
     9781 cagttgcagt cccgccatcc ataacctcca gtaagccgcc actatccaca gtagtattat
     9841 aagcgaagtc attttcttca actcgcaaac ttccgccgtt ttccaacaac atattattag
     9901 caataccatt cttgatatcg aactgaccga gacggtttgt tccgaatacc tccatggccc
     9961 gcgtggttgt tttaatagca ccgcctgttt tctgatctac cgcaagcgca aatcccccct
    10021 ctttaacctc cagtagtcct cccttttcga tagcggcaaa aacggccccc ccatcttttt
    10081 caacgattaa gcgcccgcct ttaatcgtgg tatcattcgc tgggccataa acctgtagca
    10141 caccattatt gagggtagtc tggtttgccg cgccacccat ttgaacaatt tgataaccat
    10201 catttaatac tgagtattct gcggtaccat tcatttcaat gtattgatac cctccgttaa
    10261 tttcagtatt actacttaca ccaaattctt ttatatgctg ttctccgcca ggattgattt
    10321 tcgtattatt ggcggtccca taaaccagct ggatatcttt ttccaggata acaccatcaa
    10381 ctgtctcacc atactcaact gttgatgcca atgctatggg tgaatataac gctgtagcca
    10441 ccagaacaga tattgcggaa cgacaaagag aaacagaacc agattgatgc attgagcttt
    10501 catcctatga aattaattgc agttaaaagc attgggtaca gaaaataccc atagctccat
    10561 acccggagtc agtttttaaa aactgtttaa agaaatgcac aagtattgtg attgattttt
    10621 tagttgtttt tcttgatgag aagctgatgc aaaattccgt ctttataata aaaatgatgc
    10681 caaagcgaac gacaaggttg tagttttcac tacatgtcca tacataaaat ggggtaacat
    10741 tcacgcgcct ggtagcgtta ccaacgctac gctcaaacat aatgattcta ataaaacctc
    10801 aggagactac tatgcctgaa gcaacgcctt ttcaggtgat gattgtggat gatcatccac
    10861 ttatgcgacg cggtgttcgt cagttactgg agcttgatcc tggctttgaa gtggtcgccg
    10921 aagcgggcga aggcgcgagc gcgatcgatc tggcgaatag actggatatc gacgtgatct
    10981 tgctggatct caatatgaaa ggtatgagtg gcctggatac cctcaatgcc ttgcgcaggg
    11041 atggcgttac cgcgcaaatt attatcctga ccgtatccga tgcctccagc gatgtctttg
    11101 cgctgataga cgcaggcgca gacggttatc tgttgaaaga cagcgacccg gaagtattgc
    11161 tggaagcgat tcgtaccgga gcgaaaggca gcaaagtctt tagcgaacgc gtcaatcagt
    11221 acttacgtga acgtgaaatg tttggcgcgg aagaagatcc cttcagcgtg ctgacggagc
    11281 gcgagctgga tgttctgcac gagctggcac aggggctgtc aaataaacag attgcatcgg
    11341 tgttgaatat ttccgagcag acagtaaaag tacatattcg caatctgctg cgtaaactca
    11401 atgtccgctc acgcgtggcg gccaccattc tgttcctgca acaacgcggg gcacaataaa
    11461 aatagcccga tggatttatc atcgggctga gatttatgac aaacgcaaaa ctgcctgatg
    11521 cgctacgctt atcaggccta cgtggatcga tcaatttatt gaatttacac aattttgtag
    11581 gccggataag gcgttcacgc cgcatccggc ataaactaag cgcactttgt catcagtcta
    11641 gcccgatggc atcaccatcg ggcctctttt tatttactct cctgcggcga caaatgttgc
    11701 atcgcctgcg cgatactacg ttcaatcacc gcacggcgag tatcgttggc aggtaagagt
    11761 ttcaacatca tctcccacgc ggcaacggct tcgccaaatc gctgctgctc aaaggcatta
    11821 aacgcataca tgcttagcac acggatattg ctatggtccg ttctcaccag ctgacgtagc
    11881 agttcaccgc cgaggcggtt gtcgttggga tcagatgaac gagtcaacgc ttcagcgtat
    11941 cccagtgcgg catcgctgtt cttcggatcg aggcgatacg cagtagcgta tgcatcggtg
    12001 gcgatactgg cgttacccag cgccatgcca acgcggccca acataatcca gccttctata
    12061 tctcccggat ttttttgcag ttgagtacgc atccccagcg caaggcgcga catctcttct
    12121 tcgttgagcg gatcggcttt cgggtccagc gccctgtcca gcaacgccgg agcctgtgcc
    12181 gtggcctgct gccagatttt cacctgctga taattgccag tctggtagta gctgacgcca
    12241 gccacaatta acgccaccac aatacccggc agataaacaa catatccggc acgcttacct
    12301 tccggcacgc tttgttcagg aaacgcttcc ggcaccacgc gtacccgacg ccgcgagcgg
    12361 gcgtatatga cccaaccgcc aatgccaata gccactactg gcagcaccca cagcagcacg
    12421 gtcagcggcg ttaacggcgg atcgtaagtg acgaagttgc cgtaacgcgc caccatataa
    12481 tcgacaatct cttttttact tttaccttcc tgcatcagtt catacacttt ctgacgcagg
    12541 tcggtggcaa tcatcgagtt ggaatcggca atgctgttgt tctggcattt cgggcagcgc
    12601 agttcttcag tgagctgacg gaactgttgt tcctgtgctt catctttaaa ctgcaacacg
    12661 tcgatggtcg ccagcgctga gccggagatc atcagcatca gcacgcccaa taaaaacctc
    12721 attgtgcggc ctccttactg tatttctccc acagcggctt gatctcttct tcccagacgc
    12781 gaggattgag atcgcccgca tggcgatagc gaatgatacc gttgccgtca ataaggaagg
    12841 tttctggcgc gccatagaca ccgagatcca gccctaacat gccgtcgcca tcaaacaggc
    12901 ttagcgcgta aggattgccc agctctttca gccagctgat tgccttctgg cgatcgtctt
    12961 tatagttcat gccgaccacg cgaatgccct gcgctgaaag ctgattcaga tattgatgtt
    13021 ccgcacggca ggtcggacac caagtcgccc agacgttaag cagtactggt ttgccctgag
    13081 tcagcacatc cgcctgataa aactgccccg gattgtccag tgattcgaga cgaaacttcg
    13141 gcacaggctt gccaatgagc gccgattcca gattggtcgg atcatcccct tcggcattac
    13201 gcgccagctg ccacagcagc gccgcggcaa tcgccaggaa gataatcaac ggaattaaca
    13261 atactttgcg cttcatacgg cctccggcgc agttttttgc ggactcacgc gcttacgata
    13321 gcgaggatca aacagacaca gcagtccgcc caacgccatc atcagcccgc ccgcccaaat
    13381 ccagcgaaca aatggtttgt agtaaagacg cacggcccac gcgccgtttt ccagctcttc
    13441 accgagcgcg gcgtacaggt cacgcgtgat gccgccgtca attgccgctt cggtcatcat
    13501 cgacccggca gtgttgtaat aacgtttttc cgcatacagc accgtttccg gtttaccctc
    13561 tcgcgttacg ccaatagtcg ccacaccgcc gcgccagttc gggccagtca cctctttgac
    13621 atcacggaag gtgaagcgat attcatgaat atcgacgcta tcgccggact tcatgcgcac
    13681 atcacgctca acgctataat tctggctaaa ggcaatgcca acaattgtca ctgccagccc
    13741 caggtgagcc gccaccatcc cccaataact gaaggtggtt ttcgtgccgc gtgaaatacg
    13801 cagcgcagct tccgcagttg ccagcaccgc aatccagcag gccattgcca ggccgagcac
    13861 cgtcatcgcc acaactttgc tttcgaacag ccacggcaac agcagagaca gcaccagcgt
    13921 agagatgaag gcgataatca ataaattgcg gattttacgt gggcgatcgc gcccccagcg
    13981 caccagagga ccgacaccaa gcagtagcgc aaacggcacc atcagccagg taaacatggt
    14041 gttgaagaac ggttcgccaa tcgaaatact gcccagtcca agttgcttgt gcaccagcgg
    14101 cagcagcgtc cccagcaaca ccaccagcat cgcggcgacc agcaaaacgt tgttcgctaa
    14161 cagcaaagat tcccgcgacc acagcgcatt gtttacgcgt gagcgaactt tgtgtccacg
    14221 cgcggcaaac agcagcagcg aaccgccaat caccagcacc ataaaggcga ggataaacat
    14281 accgcgcgcc ggatcagacg caaacgcgtg taccgatacc agcacgccgg aacgcacgag
    14341 gaaagtcccc aacagacaca acgagaaggc actgattgcc agcagtaatg tccacgcttt
    14401 gaagctggcg cgttgttcag tgaccgccag tgagtgcatc agtgcagtcc ccaccagcca
    14461 cggcataaac gaggcgtttt ctaccggatc ccagaaccac cagccacccc agccgagttc
    14521 gtaataggcc catgcggaac cgagcacaat gccgagcgtt aggaaaatcc acgccgccag
    14581 cgtccacggg cgagtaaaac gcgcataagt gctgtccaga cgcccgctca gcaaagaagc
    14641 aatggcaaaa gcaaacgcca ccgagaaacc cacgtacccc atataaagca gaggcggatg
    14701 gaagatcagc cccggatcct gtaacagcgg gttaagatcg cgcccttcaa tcgggaagtt
    14761 cggcagcgtg cgtgagaacg ggttagaggt aaagagaatg aacagcaaaa agccgacgct
    14821 gaccatcccc attatcgcca gtacacgggc cacaatatcc agcggaatac gctgactaaa
    14881 aatcgccacc gcaaaggtcc agccgctcat cagcagcacc cacagcagaa gcgaaccttc
    14941 atgcgcgccc caagtagccg ccacgcgata ccacaccgga agctgggtat tggagttgct
    15001 ggcaacatag gtgacggtaa agtcgttgac cacgaaagca ttgaccagca ccagaaatgc
    15061 gccagccaca gacataaaca gcagccaggc aaacaagcgg gaagacgcca tcatgcgcgc
    15121 atctccgcgc gccacgcccc atagcggata cacggacagc agcagcgcaa tccctaacgc
    15181 caagcacagc agcccgtttc caatttccgg catcatgatg ctgggtcctt ataaacactc
    15241 gccgggcgac ggtggttagc ttccatcgct ttctcaactt ctggcggcgt gtagttttcg
    15301 tcgtgtttcg ccagcacttc tttcgcgagg atatgattgc ctttttccag ctcgccctgc
    15361 accacaacgc cctgcccttc acggaacaga tccggcaaaa tgccttcgta agagacatcc
    15421 actgagcctt cagcatcgta aatggtgaag gtcactttca gcgaattggg atcgcgctgc
    15481 acgctacccg gcatcaccat cccgccaacg cgcagacgct gaccgacttc cggcatctgc
    15541 tgagtttcac gcttgccgta gagaatttcc cccggcgtat aaaagagatc gatattcgag
    15601 cgcagcgcat atagcaccag accgatagtc agcgctagcc ctgccaacac ggcacaggca
    15661 atccacaagc ggtttttacg gcgaatattc atgcagcctc ctgctgttgc gcagcacgta
    15721 aacgcgcctc acgcgcccgc tgttgcgcca caccacgcag aattgcgcga tgttgcatca
    15781 ccgagtgcac gaccaaaacc accagcggaa taacggtcat caccaccgcc agccagacaa
    15841 aaaaggcgta accgcccatt gcgaaaaatt cattccagga agcaaatgca ggggtcattt
    15901 acggcctctt ttcagtatca gttcactcac ccacggacgg cgtttttcca tcagcaaaat
    15961 caaattacgc atccgcatca gcgtcagcgt ggcagacagg agcaggaagc caaaaatcga
    16021 ccagcgcagc ggcgaacgca tcgccggatc gatactttgc tgcatccgcg ttgatccctg
    16081 atgcagggtg ttccaccact ccacggagta atgaataatc ggcagattca ccacgccaat
    16141 cagcaccatg atacctgccg cacggcccgc cagacggcgg tcgtcgaagg cgtgccacag
    16201 ggcaatcaca cccacataca aaaacagcag caccagttca gaagtcagac gtgcatccca
    16261 tacccaccag gtgccccaca tcggttttcc ccatgcagag ccggtaacca gggcaataaa
    16321 ggtaaacacg gcaccaatgg gggccatcgc cgccaccgcc aggttggcca ttttcatctg
    16381 ccagacaagg ccaataaacg ccgccaccgc cattgatgca taaatgccca tcgaccagat
    16441 cgccgcaggc acatgcaggt agataatgcg gtagctattt ccctgctgat aatcagccgg
    16501 agcaaagccg aatccccaga tccagccgac ggtaagcacg accacactgg caattgccag
    16561 ccacggtata aaccagccac agatttgata cagccgtggt gggatcgcca gttgatgcag
    16621 tgttttccac atagtttcga taccagactc gaacaaaaat cagtaatcca gcgttattga
    16681 atgctgattc gtaacgctgc cgccgtcgca aaaggactta atgtcgcggt gcctgccagc
    16741 aacgcgccta aaattgccag atacccatca acgggcaaat gcatagaagc cgcgtccatc
    16801 gcggcggtgg caaagatgag taatgggata gtcagcggta acaccagtat gctgagcagc
    16861 acaccaccgc gcttaagtcc cactgtcagc gccacgcccg gtgcaccgag aaagccaagc
    16921 gtaggcgttc ccagcagcag cgtcagcgcc atcacttgcc agccataaac atccattccc
    16981 agtagcattg ctaccagtgg cgaaaggatg agtaacggca gaccggttac catccagtgc
    17041 gccatcacct tcgccagcac aacggcgggc aagggtaacg gcaacaacat caattgttca
    17101 agactgccgt cctgcaaatc gtcacggaac agtcgttcca gcgccagcaa ggatgaaagc
    17161 agcgcagcaa cccagataat gcccggtgca atacgcgcca gcagttgcgg ctccggaccg
    17221 atactgagcg gaaaaagggt aattacaatc aggaagaacc acagcgggtt ggcgatttcg
    17281 gcgctatggc gaaacgctac gcgcagttca agacggaaaa tacgccagaa catcatgcgg
    17341 ccctcgtttg cgtcagtgaa atgcggcgaa ttttactttc agcaacgttg agcggctggt
    17401 gggtagtcag aatcacaatc cccccctgct ccgtatgctg cgccatacgc tgggtcagac
    17461 gatcgacgcc gttaacgtca atcgcggtaa aaggctcgtc gaggatccat aatgtggcac
    17521 gggtcaacca cagacgcgct aaagcgacgc ggcgttgttg cccggcagaa agttgattta
    17581 ccggaatatc ttcgaatccg gcaagcccgg cctgcgccaa ggcttccaga cattgtgcgg
    17641 catcgccatc gcgatgataa aagtgcagat tttctaacgc cgtcagccgg gttttgatcc
    17701 ccggctgatg gcctatccat aacagggttt gatggtagct gtcgcgtacc tgatgcaagg
    17761 gctgcccttg ccagagaacc tcgccagcgt cagggcgaga caaccccgtc agcaaacgga
    17821 gaagcgttgt cttccccgcg ccgttgctac cggtgatttg tacccactct cctgcgttca
    17881 gcgtaaatga caagccacta aataaggttc gttcatcccg ctcacaaagt aactctctgg
    17941 cttcaagcat acccacgcaa taaccctgtt aaaaacctgg ctcgacttca cgcatatcgg
    18001 gcagcttgtg cgcgatccct ttatggcaat caatacaggt ttgcccatct ttcaccgcct
    18061 ggtcatgcat cttcgcggca accgatttct gggcggttgt atccatatac tcgaagttgt
    18121 gacagttacg gcactcctgc gagttattgt ccttcatgcg ccgccactca ttctgtgcca
    18181 tcgtcagacg atgagcttca aatttctgcg gcgtgtcaat aacgccaaaa attttaccat
    18241 acagctcttt acttgctttg agcttgcgta tcatcttcgg cacaaactcg tgcggaacgt
    18301 gacaatccgg acaggtcgca cggacgccgc tacggttgtt gtagtgcacg gaatccatgt
    18361 attcctgata caccgtgttg cgcatttcgt ggcagctaat gcagaactct tcggtattgg
    18421 ctttttccat cccggtgtta aagccccccc agaagacgat gccgccaaca aaaccgatca
    18481 acagcagcgt ccccagcgcc agatggctgg gggtacgcca ccatttccac aggcgcttaa
    18541 tcagaccagg cttacggtca gaatttccca taataacctc ttatttcccg taaccttttg
    18601 atggggtaaa ggtattcccc acgattggcg cggtatcggc ctgcggtacg tgacattgca
    18661 gacagaaata acgacgcgga gccacttccg cgcccacttt gccgtcgctg tccataaagt
    18721 gagtaggact gatacgcggc gcgccagtgg tgcgatagct ttcgacaccg tggcactgca
    18781 agcagcgatt ggtattggtc gttacctgat aaccttcaac gctatgcggg atcattggcg
    18841 gctgattcac atagttcagc ggcatccgat cctgctcttt tggcatccga atggcccctt
    18901 cctgcgtccc ggatacttcc ggcgactggc taaaatcgac gccattagcc gcccaaaccg
    18961 cgccgcttac caccagggcc agcatcgccg tccattgaca cagcgctttc ttcaggtcat
    19021 ggcttttcat gatttcgctc ccgaactcca tcgtgtagtt attgtaaata catcctcaga
    19081 acagacatcc acgcagcgac cgcacgtcat gcaatcgcgg ctggtgacct gcaccgggct
    19141 ttgttcatcc agcaccgggg cacgtagcac atgcggttcc gggcaaacat gaaaacaatc
    19201 catacagcgg ttacatttct gccgatcgct ggccgcaacg gtaatcacac ctttgctacc
    19261 cagcacgcca tacagcgcgc caaccggaca aatgtgcccg caccagccgt gttcaacgac
    19321 cagtagatca aataaaaaca gcgcgagaat aagcagcgcg ccgctgccga agcccattac
    19381 caggctacgc cccatcagag aaacggggtt gatccattcc caaatgagcg tgccagttaa
    19441 ggctgatccc accagaatga ccaccaacag cacgtagcga atgtggcgag ggatcgtcgc
    19501 agactgattg aggtcaaagc gcctgcgtaa ccagttcgct aagtcggtaa tcgggttcag
    19561 cgggcagacc cagctgcaaa ataatcgctt tcccgccagg gcgtagagca cggtgataat
    19621 caccgcaccg gtcaacgcca ccgtggcagg cagatgacca ctggcaagac tttgcagcgt
    19681 catcagcgga tcggtcagcg gaacggtgtc gaataacagg ctactgctgt agttgccgtg
    19741 caagatccac acaccaaacc atggaccgct caaaaacatc cccagcacga agaactgaca
    19801 aaggcgacgt aacaccagcc aacggtgact gcgccaccag ccttttttct ccagcgcctc
    19861 gcgcccggcg tcacgtttac gatttgccat tgttcccctc cagccagccg aagcggtaat
    19921 ggtgacctaa ctcccctttc gccagtgaca gcggtaacac cttgattgcc ggttgttcca
    19981 gcacgcatac tttttcgcac ttaccgcaac cggtacaggc gtcgctgtga accgtcggca
    20041 gaaagcgggc gtgcttaccg gtacgcgtgt tgcgctccag ctccaaggtg atggcctcat
    20101 caattttcgg gcattcgcga taacaaacat cgcagcgcag cccctgaaag ttgagacagt
    20161 tttcctggtc caccagtacc gccagcccca tccgcgcgtc gtcgatcgat tcaatctcac
    20221 gatccagcgc accgcttggg cacactttgg cgcacggaat gtcctcacac atttcgcaag
    20281 gaatatcccg tgcgacaaaa tatggcgtgc ccgccgacag accagaggcc agcgtcgcca
    20341 gttttaaggt gtcgtaaggg caagcctgaa cacactgacc gcaacgcaca caggcactgg
    20401 caaaggcgtt ctcgtttatg gctccgggcg ggcgcaaccg cacgccagat gcgcgtgcgg
    20461 tttgctgttg taaccccagc gccacaccca cggcagccag cccgcctgct gtgcgaacga
    20521 catcgcgcag aaagcggcgg cgaccatttt gaggtttcgc tgaccgggac ataatggctt
    20581 acaccttctc cagtttgacc gcgcacttct tgaagtccgt ctctttcgag agcggatcgg
    20641 tcgcatccag cgtcagtttg ttaaccagct gtgcggcgtc gaagaacggc atgtacacca
    20701 ggccctgtgg cggacggtta cgaccgcgcg tttcaacaat cgagatcact tcgccacggc
    20761 gagaaaccac tttcactttg tcgccacggc gcagatcgcg cgctttcgca tccagcgggt
    20821 gaataaacaa gaccgcttcc gggaaggcgc ggtgcagttc cggcacacgg cgagtcatac
    20881 tgccggtgtg ccagtgctcc agaacgcgtc cggtagagag ccacaggtcg tactcttcat
    20941 ccggtgcttc cgccgccggt tcgaacggca gtgcgaagat caccgctttg ccatccggtt
    21001 taccgtagaa cttatagcct tcgcccgctt tcacgtacgg gtcgttacct tcgctgtaac
    21061 gccactgtgt ttctttacca ttcaccaccg gccagcgcag accgcgcgct ttgtggtagt
    21121 catcgaacgg tgccagatcg tgaccgtgac cgcgaccaaa ccatgcgtac tcttcgaaca
    21181 gccctttttg cagatagaag cccagctcgc gggattcatc gttcagctga tcttccgcca
    21241 gttcggatac cgggaatttg ctcacttcgg gggtggcata cagaacttcg tacagcgttt
    21301 tgccacgcag ttccggtttc ttcgccagca gctcttccgg ccatacttct tcagttttga
    21361 agcggcggga gaactggact aactgccaga gatccgattt cgcttcgcct ggcgcctgta
    21421 cctgctgacg ccagaactga gtacggcgtt cggcgttacc gtaagcgccc tctttctcta
    21481 cccacattgc ggtcggcagg atcaagtcgg cggccagcgc actgactgtc ggatacggat
    21541 cggagacgat gatgaagttg cgcggatcgc gccagcccgg catacgctct tcattgatgt
    21601 tcggcccggc ctgcatgttg ttggtacaca tggtccagta aacattcagc ttgccgtctt
    21661 tcagcgcacg gtcttgtgcc accgcatgaa gaccgatttt cgccggaatg gtgccgctcg
    21721 ggatattcca cttcttctca cagatatcac gatgtttctc gttagttacc accatgtccg
    21781 caggcagacg gtgagcaaag gtgcccactt cacgcgcagt accacacgca gaaggttgcc
    21841 cggtcaggga gaacggaccg caacccggct gggaaatttt gccggtcagc aggtgcaggt
    21901 tgtagaccag gttgttagcc cacacaccac gggtatgctg gttgaagccc atcgtccagt
    21961 aggagatgac tttcttgttc ggatcggcat acagctgcgc cagttgttct aactggtctt
    22021 tcggcacgcc ggtcatttca gcggtttttt ccagcgtata ctcagcaacg aaggctttgt
    22081 aatcttcaaa gctcatcggt tcggaggcgt cagaacccgg attcttcgct gctttttcca
    22141 gcggatgggt cggacgtaaa ccgtagccga tgtccgtcgc ccctttgcgc aggttaacgt
    22201 gcttgctgaa gaagtcctga tttatcgcat tgttttgaat gatatagttg gcgatgtagt
    22261 tcaggatcac caggtcagat tgcggcgtaa agatgatgcc gttatccgcc agctcgaagc
    22321 tacgatgctg gtaggtagaa agcaccgcca cggtgacgtt ctggttagag agacgactgt
    22381 tagtgatgcg tgaccagagg atcgggtgca tctccgccat gttagcgccc cacagcacaa
    22441 acgcgtcagc ctgctcgatg tcgtcatagc agcccatcgg ctcatccata ccaaaggtac
    22501 gcataaagcc aactactgcc gacgccatac agtgacgtgc gttcgggtcg atgttgttcg
    22561 aacggaagcc cgctttgaac agcttggacg cggcataacc ttcccagata gtccactgac
    22621 cagaaccgaa cataccgatc gattccggcc ctttttcttt cagggcggtt ttgaatttct
    22681 cttccatcac atcgaaggcc tgatcccagg tgattggggt aaattcgcct tctttgtcat
    22741 atttaccgtt tttcatacgc agcagcggct gcgtcaaacg gtctttaccg tacatgatct
    22801 tgggcaggaa atagccctta atgcagttca ggccacggtt aaccggtgcg tccgggtcgc
    22861 cctgacaggc caccacacgc ccctgctgcg ttccgaccag aacgccgcaa ccagtaccgc
    22921 agaaacggca cggcgcttta tcccatttaa tggcttcctg ctgaccaaca acggcgcggg
    22981 caacgcccgg cacgctgaga ccggcagccg ccgcagcggc cgcaacggcg ttagctttca
    23041 taaagctacg acgactgagt ttcatggtgt ttcctcacct tgctcttcct gctggtgata
    23101 aaccagcgac accgccagca cgccctctac gttgcgtact gactcaatgg tttggatcag
    23161 cgtttcgctg tcttctgctt ccaccaccac aatcaactga ccgctcggcg cgtcgctgac
    23221 agcaacttca cagccgggaa aggcgttcag ttgggtgctg atgtctgaaa ttcgttcgct
    23281 tttggcctgc acgaccaggc tgcaaacttg ccagttagtg tgcatggaga tactccgcag
    23341 ttatggctga taccggacag ctggctgcac atgctccaca tccgttgcaa agttggctat
    23401 taagttgcgc ctggtagatc ccggagagcg tcgggcgaaa gataattgcc attggctcac
    23461 agctatcctg acagcggcgg cattcaactg actgatacgc caggcacgcg tccccgatgg
    23521 tgaactgcaa atcccaggcc ctggtgtggc gcggagaaaa tagcgattcg gggcaagcct
    23581 gcgcgcaggc gtaacagaag ctgcactcgt tatttttgaa attaacgctc ggatagccac
    23641 ccgtgccgcg ttgcagaatg ttgttttcac aagcattaat acaggcgtcg cagcgggtgc
    23701 aatgggtcag aaaatgagat tcatcaccgg accagggcgg acggataccg ttactggctt
    23761 tgcgccagca accagtgagt atgccccgac gggatgcatc aatcttcaca ttgaccttcc
    23821 atcattaacg cgctctgaaa attgagagcg accaaataga ccgcataatt aataagccat
    23881 ttttatagcc gctaagatat taaaggatgt gtcaaagatg cataccccga tcggggtaaa
    23941 acctgtagca ggatcaaaag agtggcggga agcgtggcaa aaacgggctt ttgctcacat
    24001 ttcaaatggt tataaacata tttatatagc gattaattca ccagagatat ttctgctggt
    24061 ttgctccctc atcagaattt aacactaaaa gagcaggtaa aattg
//
LOCUS       DAZPEU010000047        23885 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPEU010000047 DAZPEU010000000
VERSION     DAZPEU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.047"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..576
                     /locus_tag="ACHU2L_001669"
     CDS             <1..576
                     /locus_tag="ACHU2L_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1356565.1"
                     /translation="LAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLC
                     MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLD
                     NLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEK
                     GSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="ACHU2L_001670"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="ACHU2L_001670"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR1356566.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHU2L_001671"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHU2L_001671"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR1356567.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHU2L_001672"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHU2L_001672"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR1356568.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHU2L_001673"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHU2L_001673"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1356569.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHU2L_001674"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHU2L_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR1356570.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="ACHU2L_001675"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="ACHU2L_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR1356571.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="ACHU2L_001676"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="ACHU2L_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR1356572.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="ACHU2L_001677"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="ACHU2L_001677"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR1356573.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="ACHU2L_001678"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="ACHU2L_001678"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR1356574.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="ACHU2L_001679"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="ACHU2L_001679"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR1356575.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="ACHU2L_001680"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="ACHU2L_001680"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR1356576.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHU2L_001681"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHU2L_001681"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR1356577.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHU2L_001682"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHU2L_001682"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR1356578.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="ACHU2L_001683"
     CDS             13936..14316
                     /locus_tag="ACHU2L_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR1356579.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="ACHU2L_001684"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="ACHU2L_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR1356580.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHU2L_001685"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHU2L_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR1356581.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHU2L_001686"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHU2L_001686"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1356582.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="ACHU2L_001687"
     tRNA            complement(17417..17492)
                     /locus_tag="ACHU2L_001687"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="ACHU2L_001688"
     tRNA            complement(17496..17571)
                     /locus_tag="ACHU2L_001688"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="ACHU2L_001689"
     tRNA            complement(17623..17698)
                     /locus_tag="ACHU2L_001689"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="ACHU2L_001690"
     tRNA            complement(17701..17776)
                     /locus_tag="ACHU2L_001690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="ACHU2L_001691"
     tRNA            complement(17912..17987)
                     /locus_tag="ACHU2L_001691"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHU2L_001692"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHU2L_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR1356583.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHU2L_001693"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHU2L_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR1356584.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHU2L_001694"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHU2L_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR1356585.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHU2L_001695"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHU2L_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1356586.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHU2L_001696"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHU2L_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR1356587.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHU2L_001697"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHU2L_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR1356588.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHU2L_001698"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHU2L_001698"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1356589.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
BASE COUNT         5902 a         6313 c         5858 g         5812 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaatgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agctgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaaa gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta
    23881 gagcg
//
LOCUS       DAZPEU010000048        23563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPEU010000048 DAZPEU010000000
VERSION     DAZPEU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.048"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            21..518
                     /gene="ftnA"
                     /locus_tag="ACHU2L_001699"
     CDS             21..518
                     /gene="ftnA"
                     /locus_tag="ACHU2L_001699"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR1356590.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(555..794)
                     /locus_tag="ACHU2L_001700"
     CDS             complement(555..794)
                     /locus_tag="ACHU2L_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR1356591.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            984..2195
                     /gene="tyrP"
                     /locus_tag="ACHU2L_001701"
     CDS             984..2195
                     /gene="tyrP"
                     /locus_tag="ACHU2L_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR1356592.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2257..2922)
                     /gene="yecA"
                     /locus_tag="ACHU2L_001702"
     CDS             complement(2257..2922)
                     /gene="yecA"
                     /locus_tag="ACHU2L_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR1356593.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3118..3204)
                     /locus_tag="ACHU2L_001703"
     tRNA            complement(3118..3204)
                     /locus_tag="ACHU2L_001703"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3168..3170),aa:Leu,seq:taa)
     gene            complement(3217..3290)
                     /locus_tag="ACHU2L_001704"
     tRNA            complement(3217..3290)
                     /locus_tag="ACHU2L_001704"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3256..3258),aa:Cys,seq:gca)
     gene            complement(3345..3420)
                     /locus_tag="ACHU2L_001705"
     tRNA            complement(3345..3420)
                     /locus_tag="ACHU2L_001705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3385..3387),aa:Gly,seq:gcc)
     gene            complement(3572..4120)
                     /gene="pgsA"
                     /locus_tag="ACHU2L_001706"
     CDS             complement(3572..4120)
                     /gene="pgsA"
                     /locus_tag="ACHU2L_001706"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR1356594.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4177..6009)
                     /gene="uvrC"
                     /locus_tag="ACHU2L_001707"
     CDS             complement(4177..6009)
                     /gene="uvrC"
                     /locus_tag="ACHU2L_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR1356595.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6006..6662)
                     /gene="uvrY"
                     /locus_tag="ACHU2L_001708"
     CDS             complement(6006..6662)
                     /gene="uvrY"
                     /locus_tag="ACHU2L_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR1356596.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6804..6875
                     /locus_tag="ACHU2L_001709"
                     /pseudo
     CDS             6804..6875
                     /locus_tag="ACHU2L_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            6958..7134
                     /gene="yecU"
                     /locus_tag="ACHU2L_001710"
     CDS             6958..7134
                     /gene="yecU"
                     /locus_tag="ACHU2L_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR1356597.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            7121..7345
                     /gene="yecF"
                     /locus_tag="ACHU2L_001711"
     CDS             7121..7345
                     /gene="yecF"
                     /locus_tag="ACHU2L_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR1356598.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7378..7998)
                     /gene="sdiA"
                     /locus_tag="ACHU2L_001712"
                     /pseudo
     CDS             complement(7378..7998)
                     /gene="sdiA"
                     /locus_tag="ACHU2L_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(8228..8980)
                     /gene="tcyN"
                     /locus_tag="ACHU2L_001713"
     CDS             complement(8228..8980)
                     /gene="tcyN"
                     /locus_tag="ACHU2L_001713"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR1356599.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(8977..9645)
                     /gene="tcyL"
                     /locus_tag="ACHU2L_001714"
     CDS             complement(8977..9645)
                     /gene="tcyL"
                     /locus_tag="ACHU2L_001714"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR1356600.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9660..10646)
                     /gene="dcyD"
                     /locus_tag="ACHU2L_001715"
     CDS             complement(9660..10646)
                     /gene="dcyD"
                     /locus_tag="ACHU2L_001715"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR1356601.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10751..11551)
                     /gene="tcyJ"
                     /locus_tag="ACHU2L_001716"
     CDS             complement(10751..11551)
                     /gene="tcyJ"
                     /locus_tag="ACHU2L_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1356602.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(11639..12190)
                     /gene="fliZ"
                     /locus_tag="ACHU2L_001717"
     CDS             complement(11639..12190)
                     /gene="fliZ"
                     /locus_tag="ACHU2L_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR1356603.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(12236..12955)
                     /gene="fliA"
                     /locus_tag="ACHU2L_001718"
                     /pseudo
     CDS             complement(12236..12955)
                     /gene="fliA"
                     /locus_tag="ACHU2L_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(13120..14772)
                     /gene="fliC"
                     /locus_tag="ACHU2L_001719"
     CDS             complement(13120..14772)
                     /gene="fliC"
                     /locus_tag="ACHU2L_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR1356604.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            15038..16444
                     /gene="fliD"
                     /locus_tag="ACHU2L_001720"
     CDS             15038..16444
                     /gene="fliD"
                     /locus_tag="ACHU2L_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1356605.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            16469..16879
                     /gene="fliS"
                     /locus_tag="ACHU2L_001721"
     CDS             16469..16879
                     /gene="fliS"
                     /locus_tag="ACHU2L_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR1356606.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            16879..17244
                     /gene="fliT"
                     /locus_tag="ACHU2L_001722"
     CDS             16879..17244
                     /gene="fliT"
                     /locus_tag="ACHU2L_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR1356607.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            17322..18809
                     /gene="amyA"
                     /locus_tag="ACHU2L_001723"
     CDS             17322..18809
                     /gene="amyA"
                     /locus_tag="ACHU2L_001723"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1356608.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(18843..19256)
                     /gene="yedD"
                     /locus_tag="ACHU2L_001724"
     CDS             complement(18843..19256)
                     /gene="yedD"
                     /locus_tag="ACHU2L_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR1356609.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            19443..20648
                     /gene="yedE"
                     /locus_tag="ACHU2L_001725"
     CDS             19443..20648
                     /gene="yedE"
                     /locus_tag="ACHU2L_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR1356610.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            20645..20878
                     /gene="yedF"
                     /locus_tag="ACHU2L_001726"
     CDS             20645..20878
                     /gene="yedF"
                     /locus_tag="ACHU2L_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR1356611.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            20985..21655
                     /locus_tag="ACHU2L_001727"
                     /pseudo
     CDS             20985..21655
                     /locus_tag="ACHU2L_001727"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(21695..22903)
                     /locus_tag="ACHU2L_001728"
                     /pseudo
     CDS             complement(21695..22903)
                     /locus_tag="ACHU2L_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            22911..23337
                     /gene="tnpA"
                     /locus_tag="ACHU2L_001729"
                     /pseudo
     CDS             22911..23337
                     /gene="tnpA"
                     /locus_tag="ACHU2L_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         5770 a         6088 c         5698 g         6007 t
ORIGIN      
        1 aacctttgtg gagcactatc atgctgaaac cagaaatgat tgaaaaactt aatgagcaga
       61 tgaacctgga actgtactct tcactgcttt atcagcaaat gagcgcctgg tgcagctatc
      121 ataccttcga aggtgctgcc gcgttcctgc gccgtcacgc ccaggaagag atgacgcata
      181 tgcagcgtct gtttgattac ctgactgata ccggcaattt accgcgtatt aataccgttg
      241 aatctccgtt tgctgaatat tcctcacttg atgaattatt ccaggaaacc tataaacacg
      301 aacaattaat cacccagaaa attaacgaac tggctcatgc tgcaatgacc aatcaggact
      361 acccaacatt taatttcctg cagtggtatg tttctgagca gcatgaagaa gagaaactgt
      421 tcaaatcgat tattgataaa ttaagcctgg caggcaaaag cggcgaaggt ctgtatttta
      481 tcgacaaaga actctctacc ctcgacacac aaaactaatg ctctccggcg gcaggtttct
      541 gccgccgttt caaatcagtg acggcaaatc ttactctgat cggtcgaaaa accttcttct
      601 gaaggtataa atttgccttt tgctgccaga aacgctacca gctctcccgc tgtcacccct
      661 tctgccgagc acgtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat cgcagcttcc
      721 agactggcat gcgtatattg ctcgcctgat tcaatcatca tatttaacac ttcatgaccg
      781 tgaatagagt ccatcgttcc tcctcaaaaa aagcctagcg tagcgattac cgcttatgaa
      841 gactttgcgc cagcgcagga ctgaatgctt ttattgtaca tttatattta cacaatatgt
      901 aacgtcggtt tgacgaagca gccgttatgc cttaacctgc gccgcagata tcactcataa
      961 agatcgtcag gacagaagaa agcgtgaaaa acagaaccct gggaagtgtt tttatcgtgg
     1021 cgggaaccac aattggcgca ggcatgctgg caatgccgct ggctgcggcc ggtgttggtt
     1081 ttagcgttac gttaatcttg ttgattgggc tttgggcgtt gatgtgctac acggcgctat
     1141 tactgctgga ggtgtaccag catgttccgg cagataccgg tctgggcacg ctggcaaaac
     1201 gctatctggg acgctacggt caatggctga cgggcttcag tatgatgttc ttaatgtatg
     1261 ctctgactgc agcatacatc agcggtgccg gtgaattgtt ggcctccagc atcagcgact
     1321 ggacaggtat ttcaatgtcg gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg
     1381 gcgtggtttg tgttggaaca tcgctggtcg atttatttaa ccgttttctg ttcagcgcca
     1441 aaattatttt tctggtggtg atgctggtat tgctgctacc gcatattcac aaagtgaatc
     1501 ttttaaccct gccgttgcaa caggggctgg cactgtctgc aatcccggtg atttttacgt
     1561 cgtttggttt tcacggtagc gtgccgagta ttgtcagcta tatggatggc aacattcgta
     1621 agctacgctg ggtgtttata atcggtagtg ccatccccct ggtggcatat attttctggc
     1681 aggtggcgac gcttggcagc attgattcaa caacctttat gggattgctg gctaatcatg
     1741 ctggattaaa cgggctgtta caggcgttac gcgaaatggt ggcctctccg catgttgagc
     1801 tggcggtgca tttatttgct gatttagccc tcgccacgtc atttctcggc gttgcgttag
     1861 gcttatttga ttatctggct gatttgtttc agcgttcaaa taccgttggt ggacgattgc
     1921 aaactggtgc aattaccttt ctgccgccgt tggcgtttgc actgttttat ccacgaggat
     1981 ttgtgatggc gctgggttac gccggtgtgg cgctggcggt actggcattg attatccctt
     2041 cactattgac ctggcaaagc agaaagcaca atcctcaggc gggttaccgg gtcaaaggtg
     2101 gtcgtccggc gctggtggtg gtgtttctct gtggtattgc tgtgattggc gtgcaatttt
     2161 tgattgcggc agggttgtta ccagaagtgg ggtgatcaga tagcctcaaa ttccttattg
     2221 ggtgccagaa ttaacgctga cacccaatgt gacctcttaa tgcaggcagc actgcttaaa
     2281 tttcttacca ctgccgcacg gacaaggatc gttacgcccc ggtttctctt ctgctttgat
     2341 cggttgctga acagcttttt cctgcggatg cgccatccag tatgcatgta aatcaagcgc
     2401 cgccagtcga atggcatcta cgctctcttc aaacgcttct ggcgacattt tctctacccg
     2461 ctcgaagttt tcctcagtac cgtgcagcgc aatcgcctcc agcgctggtt ttaaagaatc
     2521 gggcaacgtt gaccagtcag aaagtgccac gccccgcata tagccaaagc accactcctc
     2581 aacaatcgtc agctcgctgc catcaacttc tcgcaagccg aataacggct caaactgctc
     2641 cgggaattcg ttcagacgct ctgcggtatc ggccatatgt tgaaaagcca ggttcataaa
     2701 gcgcgtcatc tctttctctg acgcccaacg cggcacatag tcagccccac cccacacggc
     2761 aaccagccac tgttctggtt caatctcttg cggagaactc aacaccgccg tcaataaacc
     2821 gtccagctcc gccacatcaa ggatggcgtg gtcagtgttg tatttggtca gaatatcgtc
     2881 cagccattcc aactcacttt cgtttaacgg tcccgttttc atacgctttt ccttgtggat
     2941 ctcaactcgc cagcacctat cttacatgcc ggtccgtatc agagatactt tttgagtggc
     3001 tttgccggtg attaaaaatt aaggagggtg taacgacaag ttgcaggcac aaaaaaacca
     3061 cccgaaggtg gtttcacgac actgcttatt actttgattt tattcttatc tttcccatgg
     3121 tacccggagc gggacttgaa cccgcacagc gcgaacgccg agggatttta aatcccttgt
     3181 gtctaccgat tccaccatcc gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac
     3241 cggactagac ggatttgcaa tccgctacat aaccgctttg ttaacgcgcc aaattcttca
     3301 ggcctttcag ccagacatcc gcttgacgcc gatgtctttt aaactggagc gggaaacgag
     3361 actcgaactc gagacctcga ccttggcaag gtcgtgctct accaactgag ctattcccgc
     3421 attcatcaag caatcagtta atcacttgat tttattatcg tctggcaatc agtgccgccg
     3481 ttcgatgcgt tgcattctac ttacctggcg cgatgagtca acgatatttt tcaccacttt
     3541 tgatcgtttg ctgaaaatta cgccgaaacg atcactgatc aagcaaatct gcacgcgcag
     3601 cgctcaaata ttgcaacatt gaccacagag tcagtaccgc agccacaaag aaaagtgcaa
     3661 taccgacgta ctcaacccaa atgttcggac gccacagcag ccaggccaac gccaccatct
     3721 gggcagtggt tttcactttc ccaatccagg agacggccac gctactgcgt ttacccaact
     3781 ccgccatcca ttcgcgtagc gcagaaataa taatttcacg ggcgatcatc gttgccgccg
     3841 gtaatgtcac ccaccagctg tgataatgct cggttaccag caccatggcg atagccacga
     3901 gaactttatc tgccacaggg tcaaggaaag caccaaaccg ggtactctgg ttccagcggc
     3961 gtgccagaaa accatcgaac cagtcagtca ccgccgcgac gcagaaaatg agcgcggcgg
     4021 caaacggcga ccaggtgaca ggcagataaa agaccaatac aaagaatggg ataaggatga
     4081 cacggaacag tgtaagcaac gtagggatat taaattgcat aatgacgggt aactatctgt
     4141 tgtcagtaag attaccccta tgttgctaca gagacatcaa tgtttcaacg accagaagat
     4201 cttttctgcc agaccttgcg aaatacccgg cacttttgca atttcctcga cgctggcgtt
     4261 acgtaaacct tgcaaaccgc ccatatattt caacaacatt tgccgacgtt ttggcccgac
     4321 gccttcaatg gtttccaggg aactggtatt tttgaccttc gcccgttttt tacggtgccc
     4381 gccaatcgcg tgatcatgtg attcatcgcg aatatgctgg ataacatgca gcgcgggtga
     4441 atctggcggc aaactaaatc cctcaccttc cggctcaaag aacagcgttt ccagtccagc
     4501 cttacgatct gctcctttgg caacgccaag tagcagcgga tgatttttat cccatgagac
     4561 atccagttcg gcgaagacat ttttcgcctg cgcaagctgg cctttgccgc cgtcgataag
     4621 gatcacatcc gggatcttac tgtcgtcaat ggctttacca taacgccgac gcagcacctg
     4681 attcatcgcc gcataatcat cgcccggcgt gatgcctgta atgttatagc gccgatactc
     4741 cgcacgcagc gggccgttag catcaaacac cacacaggaa gcgacggttt gttcgcccat
     4801 ggtatggctg atgtcaaagc actccatccg cttcacttcc ggcaatttca acacgctggc
     4861 aagcgcggtc agtcgctggt gaacggtaga ttgctgcgaa agtttgctgg ttaaggccgt
     4921 cgccgcattg gtgcgcgcga gtttcagata acgcgcccta tcgccgcgag gtttggtttg
     4981 aacattaatc ttgcgtcccg ccagttctga aagggaatcg gcgagcagcg ttttatcgct
     5041 aagattaaaa tcgagcagga tctcacccgg taaggtgcgc atctggctgc cttgtaaata
     5101 gaactgacct acgaaggttt ctaccacctc gctcagttcc gtaccgccag gcactttcgg
     5161 gaaatagctg cggctgccga gcactttgcc ctgacgaatg aacaatacgt ggacacaagc
     5221 catgcccgca tcgaacgcca caccaataac gtcaaggtcg tcgccggtat tggaaacaaa
     5281 ctgtttttcg gtgacgcgtc gcaccgcctg aatttggtcg cgaatacatg ccgcttcttc
     5341 aaactccaga ttctggctgg cagtttccat acggctaatg agttgcgtaa gcacctgatc
     5401 atctttgcca gacaaaaaca ggcgcacata ctcgacctgc tgagcgtatt cttcttcact
     5461 caccagtcct tcaacgcacg gtcccagaca gcgccctatc tggtattgca gacacggacg
     5521 cgagcgattg cgataaacac tattttcgca ctggcgaatg gggaaaatct tttgcagtag
     5581 cgccagtgtt tcacgtacgg catagccatt cgggaacggg ccgaaatatt cacctttggc
     5641 atgctttgca ccacgatgca tcgccagacg cgggtgggta tcgccactca ggaagataaa
     5701 aggataagat ttatcatcgc gtagcaaaac gttgtaacgc ggctgataga gtttgatgta
     5761 gttgtgttcc agcaacagcg cttcggtttc tgtatgcgta accgttacat caatttgctg
     5821 gatctgggcg accagcgctt cggttttgcg cgaagcgagg ttgctgcgga aatagctgga
     5881 aagccgtttt ttcaggtctt tcgctttgcc gacatagata accgtaccac cagcatcgta
     5941 catgcgataa acgcctggct ggctggttac ggtttttaaa aacgcttttg cgtcaaactg
     6001 atcactcact gacttgataa tgtctccgca ttacacagac catggcgaat tgccaggtga
     6061 gtcagctcaa catcgccatg aatgtttagt ttactgaaca tacgatagcg gtagctgttc
     6121 accgttttcg gactgagatt gagctgttct gagatctcat tgaccttctg gcccttagtg
     6181 atcatcagca taatctgcaa ttcacgttca gacaaactgg caaatgggct ttctgttttt
     6241 tctggttcga tctggcttaa cgccatttgt tgagcgatat cagaagcaat gtaacgctgc
     6301 cctgaataga cagaacgaat cgcactcacg acttcctgcg gagccgcgcc tttgctgagg
     6361 tagccagcag cacctgcctg catgactttc gctggtaaag ggttttctgt atggaccgta
     6421 agcatgatga ttttaacatc agctgtggaa cgcgcgattt tacgcgtcgc ctcaagaccg
     6481 ccaatgcccg gcatactcat gtccattagc accacgtcaa cggcatttgt ccggcaccac
     6541 ttaacggcgt cttcaccgca cgatgcctct ccgacgactt ttataccctt aatatcttcc
     6601 agaatgcgtc gtatccctgc gcgcaccagt tcgtggtcat caacaagtag aacgttgatc
     6661 aaaggaatat ctccagaaat agggataacg ctactgatag ttagtcattc gtatattagc
     6721 ggtttttatt gcaactttga aacgttaaaa atggtgggta ttcgattttt ctctcgtttt
     6781 tgtcaattcg attgtccaca gttatggtca cgcccgtcac caacggctta ccggatcgtt
     6841 tcacagtaag ccctacgatg aaatgtgaca aaaattgact ttattcagca aaaatgaaaa
     6901 tcagcccgct gaatatgtaa cattaattaa cccgaacaat acgggcaaaa aacattcatg
     6961 ataaaaatat ttattggtca ttatattaac gtattttata gcactgccga tatcacgctc
     7021 aaaaaacaac cactgctatt tttagcaaag cttatggtat actccgccgc cttaacattt
     7081 ttcaccacaa attttcattg caacatgacg aggaaaataa atgagtacgc ctgatttttc
     7141 tactgccgag aataatcaag aactggcaaa tgaagtctcc tgcctgaaag cgatgctgac
     7201 gctgatgctg caggcgatgg gacaagctga cgcgggccgc gtgatgttaa agatggaaaa
     7261 acagcttgcg ctgatcgaag acgaaaccca ggctgcagta ttttccaaaa cggttaagca
     7321 aattaaacaa gcctaccgtc agtaatataa aaccggctga gagcgtgcct ttcagccggt
     7381 tattgacaaa atcatcgcta tctctgctga tgtcttccct tccgccgtcc atttcagaat
     7441 ttctttttcg cgcttgctga aattcatctc tggcgtcatc actatttcat catttaaacg
     7501 catcagagcc atcagacttt cgcgcaccag taactgcatt tttaattgca gttcatcact
     7561 aagaatgggt atttcgcgcg tgctgcaacg ggaaaaggac aaaaagccca gcgcccgatt
     7621 tggcagcatt aaatactgag tgacaccgcg gcgtaaacca tgtgcgtgcg cggcttccca
     7681 taatggctgt gcttcgctga ataagtcatc attccacatt aaatggccct gactaaagtt
     7741 ttcagggttc agcacaggat caattgcgag aaaatttttt gcctgataat aactaaccca
     7801 cgactcaggg taattggtgt agaaagccac tttaggtcgc gtgaatggta ccgggtggcg
     7861 aacacataac gaatagtaat cgtactccag ctgctgagcc tgaagctcta tttcatggta
     7921 ggcctcttct gcggcctcca tcctctgaaa acgcaacagc atcgtgcgac gccagctgaa
     7981 aaaatccgta tcctgcatag taaaccgcaa cgcccctgag agtgagtatc atttataatg
     8041 ataattcaaa ctatcacata aataagattt atatataatt tatattattc aggcaatgaa
     8101 ttacttttgc aagccatcgc attctcttat gttattaatg agttatgctg atttgttaag
     8161 cagttttatc acgcttgaaa tggcgtcccg ccccgacagg tgaatcgtcg gggctgattt
     8221 tttcttatta ttgcagcaga agcttctcga ggaactggcg ggtgcgaggc tgctggggat
     8281 cggcaaataa cgcttttgct gccccctgct cgactatccg cccctggtcc ataaagatcg
     8341 cccggtccgc aacatcccgg gcaaagctca tttcgtgtgt cacaatcacc atcgtgcgct
     8401 tttcctgcgc cagctggcgg atggtgttca ggacttcgcc caccagctct ggatccagcg
     8461 ccgacgttgg ctcgtcaaac aaaatcacct caggacgcat tgccagtgca cgcgcaatcg
     8521 caacacgctg ctgttgaccg ccagacaaac gacgtggata gctggtttct ttaccggcca
     8581 gcccaacttt cgccagcagc tcgcgagcgc gcgccgtggc ctcttctttc ggttcgcctt
     8641 tgacgatcac cggcccttca ataatgtttt ccagcaccgt acgatgcgga aacaaattaa
     8701 agttctggaa gacaaacccg acgtgctgac gcaactggcg aatcagcgat ttttgctgac
     8761 ttaatgaacg tgcagtatca atagtgatat cgccaacggt gatcgttccc gcttccggtt
     8821 gttccagcag atttatgcta cgtagcaatg tggttttgcc ggaaccactc ggaccgataa
     8881 ttgccaccac ttcgccaggc tttacctcga ggtcgatacc gtgcagcacc gtctgaccgt
     8941 ggaatttttt caccaggttc ttaacttcaa tggcactcat tttggttctc tctcctggcg
     9001 attaagttga ttctcaaaat ggttctgcaa cgtcgataac acagtcgcca tgatccagta
     9061 gatgagcgaa gccgccaggt acatggtgaa cacctccagc gtgcgcgagg taatcaactg
     9121 cgcctgacgg aacagctccg gcacctggat tgtcgcggcc agcgacgtat cttttaccag
     9181 actgataaag ctgttcgaca gcggcggcaa cgccacccgc gccgcctgtg gcaaaatcgc
     9241 gcgacgcatg gtctgccacg gcgtcatacc aatactggca gcggcttccc actgaccttt
     9301 atcaatagaa gaaatggcgg cgcgcagcgt ttcggcggca taggcggcag tgttcagcga
     9361 cagaccaatc attgctgacg gaatgggatc taattcgata ccaaactgcg gcaggccgta
     9421 atagatcata aacagttgtg cgatgagcgg cgtaccacga aaaatagaga tataaaaacg
     9481 cgccagccag cgcacgggcc agattggcga caggcgcatc agcgcgagaa taaacccgag
     9541 tagtaagcca aaaaacatgc cgccgatact gagttgcagg gtatacccag cccctttcaa
     9601 caggaacggc aaggaatcaa taaccagttg tatactttct tgcatgagcg ttcccggagc
     9661 taaacgtggg gatgataggc gaacagcgca ggcgcgccgc cggtatgaat aaacagaatc
     9721 ggcccttcat ctttgaagcg tttctgactg ataccgtcaa tcagccccgc catcgctttt
     9781 ccggtataca caggatcaag cagaatgcct tccagacgcg ccagcaattt cactgcttcc
     9841 atgccttcgt cgttcggcac gccgtagcca ggtgcaaaat agtcatccca gagtaaaatt
     9901 tccgctgatg cggtcagctc cagttctttc gcaatcgcct gttgtaggtt aaccactttc
     9961 ggcaattgat cggcaacgga acgcgacacg gtcacgccaa tcagttcgct ttcaggcatc
    10021 aggtgttcca gcccaacagc cagtccggcg tgagttccgg cactgcccga tgcgactacc
    10081 accgacgaaa tattaaccgc cccttcacac tgttgcgcga tttccagcgc actctccaca
    10141 taacctagcg cgccaagagc attagaaccg ccaaccggaa tgacatacgg gcgaaagcct
    10201 tgtgcttcga ctcgcgtcgc cagttcttcc agttgggcat tgggatcggt cagtgcgtcg
    10261 cacatttcaa tctgggtatt gaacagatcc agcaacaaac gattgccgtt ggttaaatag
    10321 ttttctgcgg ttgtgccaat aggattttcc agcagcgcca cgcagtgcag accgagtttc
    10381 gccgcgactg cggcagtctg gcgcacatgg ttagactgga tcgcgccggc agtaatcagc
    10441 gtatcggcac cttcgcgcag ggcatctgcc gcgagaaatt ccagcttacg taatttattg
    10501 ccgcccattg ccatgggggt gacctcatcc cgtttgatga aaatttcccg tccaagataa
    10561 tcagaaaagc gcggcagata ttcgagcggc gttggcgcgc cgataaactc caaccgtgga
    10621 aaacgggtta aattatgcag tggcataaca gcctccgatg tgtgttgttg tgattttctt
    10681 attatgcacg ctgaaaacgc gtaaataaaa aaggcgctag tgaaagcgcc cttttttgtc
    10741 attatgctga ttatttggtc acatcagcac caaaccattt ttcggaaaga gcttgcagag
    10801 tgccgtcttt ttgcatttcc gcaattgcat cattcaccgc tttcagcagg tcttcatttc
    10861 ctttacgcag cgccacgcca gactcctgac gggagaatgc ttcaccggtt actgccagcg
    10921 tatcgttggt tttcttcacc agatccagcg ccgccagacg atcaacgagg atcgcatcga
    10981 tacgccctac gcgcagatcc tgatatttgg tcgggtcatc atcataggta cgcacatcga
    11041 caccctgaac attctgccgc agccactctt catagttggt gcccagaccg acacccactt
    11101 ttttgccttt cagatcagca gccgttttaa tggtgccttc gttacctttt ttcaccagcg
    11161 cctgaatacc ggaaatggtg taaggggtag agaaatcgta ttttttcttg cgctcgtcag
    11221 aaatggtgac ctgattaatc accacatcaa tgcgtttaga gtccagcgac gccagcatac
    11281 cgtcccattt ggtcggtttt agtgacgcct caacgccaag atgttttgcc agctgttggg
    11341 caaattccac ttcaaaaccg gttaatttgc cgtcatcccc ctgaaaactg aacggcggat
    11401 aagtgccttc cagccctacc agcagcgtgc cgctctcttt gactttatta agcagacctt
    11461 catctgcaaa acttttaacg ctcatgcccg caaccagcgc cacggccatc acacccatca
    11521 atgcctgacg tcccagatgt gctaatttca tattcacccc gaatgttgtt atgtctgttt
    11581 gcagtgtaga gccattgttt gtaaacacaa aaacaactcc gctacatctt attcttattt
    11641 aatatatatc agaagcaggc aggctggagg atttctgcac aagtccggta caggtttggc
    11701 gctgatagtg ttgatacttc cgtaatgcga tgcggtaatt gttggtgctg gtggtgggca
    11761 gccacggggc gaggatttca tcaaggaaac cgtcctgcag ctgatcgagg gaaatatttt
    11821 gctcatgtag atgattaccc aaacggcgca gacgaacgac atattcacgc accgtacctg
    11881 ggctcatttc agtttgctca aaaagaaatt tcttaaagcc gataatatcg aaaaaatcac
    11941 tctgcgtttt gcaatgtaaa tcaccgcaaa atcggcacaa tgccgcccat gattgttgtt
    12001 ccgtttgcca gcctttttca tcaagcagcg tgtccaggcg ggaaatctcg attttattca
    12061 ctattttgcc gtcgcgaact aaggtaatgc gatcgagtaa tttacggcaa tgcgcgcaat
    12121 gggtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt ttcaggtgct
    12181 gcaccatcat taagaactcc tggtagtcaa agttaaagtg cggcatttac tgacgttata
    12241 acttacccag tttagtgcgt aaccgtttaa tgtcctggct gtgtaactga ctgacccgcg
    12301 attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc tggtaataga
    12361 gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc acccgctggc
    12421 gcagattact gtccagcagt tgttgtagcg ggttttctcg ctgatgatca tcagtaacca
    12481 gttcgatgct atcgccgtgc tcttcacgcc actcatcgta ggagaagagc tggctgttat
    12541 tggtgtcgag cagcatttga cgataatcgg caatatcgat ccctaaacgt tccgctacct
    12601 cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc tgtgccactt
    12661 cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt tcatccagca
    12721 tagcgccacg gatacgctac actgcgtaag ttgtaaatgc cgttccttgt agggcgtcat
    12781 agcgttcgac agcattaagt aacccaatgc cgcccgcctg tagcagatcg tcaagttcca
    12841 cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc gggacataac
    12901 gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa ttcacgataa
    12961 acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggg tttttaatcg
    13021 gacgattagt gggtgaaatg aggggttatt tgggggttac gcaaaaagcc gaatgtaaaa
    13081 aaccccgccg gtggcggggt ttgagaggca ttcagacgat taaccctgca gcagagacag
    13141 aacctgctgc ggtacctggt tggcttttgc cagcacggag ttaccggcct gctggataat
    13201 ctgcgctttc gacatgttgg acacttcggt cgcatagtcg gcgtcctgaa tacgggactg
    13261 cgcttcagac aggttggtag tggtgttgtt caggttggtg actgcggaat ccaaacggtt
    13321 ttgcaccgca cccagggagg agcggaattt gtcgatggag ctgatggctt catccagagc
    13381 tttcagggga tcggctgtcg tagctgcttt tgtttcagca tctgtcgtca atttaccatc
    13441 agcatcttta taaactgcct taccgctgcc gttagtcact gaaccatcat cctgtaggta
    13501 aaaatctaca gtatcagatg tggtcaacgc ctcgttgtta tcaacataaa ccgaatcaac
    13561 accttttgtt acagcgccag ttgtgccgtt gacgctataa cctgcagtag tgaaatcaac
    13621 attgtttccc gaggttgata cagctttatc gaaagctgcc tgatcaactt ttgcacctgt
    13681 tactgaataa gtaatagtgt ccggcgtagt actattaccg gtaaaggaga tagaggtacc
    13741 gccaatgtca atggttgtcg ctttggtatt attggctgca agtgtggaca gactagcctc
    13801 agtcaaagaa gcaccagagc cgtttttcgt taagcgccca gttgtatcaa tgtagagttt
    13861 atctccattg cttgccgtaa ttttaccatc actgctgatc tgaacatcct gtgcagaacc
    13921 atcgatagta atagtgccct tagcggtatc accaactccc ggggtcaaat atttctgaac
    13981 atcggcagct gaagccgttg tggtatcaaa agagtatgac ttacttgcac tgtcatactt
    14041 gtaattcgtt gcactagccg ccgttccgaa gccgttatta atggttgcct gaacagtatc
    14101 accatcacta actccagcca gcaaatcaga cgctttagca gcatcgtaac ccgcactcac
    14161 agtatatttg ttgccgacca ctgttgcatt agcagctacc aagtcagctt tagatgcagc
    14221 agtattagcc acagccccgc caccattcac attaaaccca ttcagcccca gcgtatcaga
    14281 gtcaattttc ttcagatcaa tagtgatagt ctggccgtca ttcgcaccaa cctgaatttt
    14341 catcgaaccg tcttttgcca gtacgttcac gccgttgaac tgggtctgac cggatacgcg
    14401 gtcaatttcg tcgagacggg atttgatttc gtcctgaatg gagtccagat ccgaatcaga
    14461 gttagtcccg gtagaagcct gaaccgtcag ctcacggata cgctgtaagt tgttgttgat
    14521 ttcggacagc gcgccttcag tggtctgtgc aacagaaata ccgtcgttgg cgttacgtgc
    14581 agcctgagtc aggcctttaa tgttagaagt aaaacggtta gcaatcgcct gacccgcggc
    14641 gtcatccttc gcgctgttaa tacgcaagcc agaagacaga cgctcgatag aactcgacag
    14701 cgcagactgg ttcttgttga tattattttg agtgatcagc gagaggctgt tggtattaat
    14761 gacttgtgcc atgattcgtt atcctatatt gcaagtcgtt gattacgttt tgggtttcca
    14821 cccgtcggct caatcgccgt caaccctgtt atcgtctgtc gtaaaacaac ctttagaatt
    14881 tttttcacaa acagccattt tttgttagtc gacgaaatac tcttttctct gccccttatt
    14941 cccgctatta aaaaaaacaa ttaaacgtaa actttgcgca attcaggccg ataaccccgg
    15001 tattcgtttt acgtgtcgaa agataaaagg aaatcgcatg gcaagtattt catcgctggg
    15061 agtcgggtca ggtctggatt taagttcaat ccttgatagc ctcaccgccg cgcaaaaagc
    15121 gacgctaacc cctatttcaa atcagcaatc gtcgtttacc gctaaactta gcgcctacgg
    15181 tacgctgaaa agcgcgctga cgactttcca gaccgccaat actgcattgt ctaaagccga
    15241 tcttttttcc gccaccagca ccaccagcag caccaccgcg ttcagtgcca ccaccgcggg
    15301 taacgccatc gccgggaaat acaccatcag cgtcacccat ctggcgcagg cgcaaaccct
    15361 gaccacgcgc accaccagag atgatacgaa aacggcgatc gccaccagcg acagcaaact
    15421 caccattcaa caaggcgacg acaaagatcc gattaccatt gatatcagcg cggctaactc
    15481 atcgttaagc gggatccgtg atgccatcaa caacgcaaaa gcgggcgtaa gtgcgagcat
    15541 cattaacgtg ggtaacggtg aatatcgtct gtcagtcaca tcaaatgaca ccggccttga
    15601 taatgcgatg acactctcgg tcagcggtga tgatgcgcta caaagtttta tgggctatga
    15661 cgccagtgcc agcagcaacg gtatggaggt ctcggtcgcc gcccagaatg cgcagctgac
    15721 ggtcaacaac gtcgccatcg agaacagcag caacactatc agcgacgcgc tggaaaacat
    15781 cactctgaac ctgaacgatg tcaccacggg caaccagacg ctaaccatca ctcaggacac
    15841 ctccaaagtg caaacggcga ttaaagactg ggtgaatgcc tataactcgc taatagatac
    15901 cttcagcagc ctgaccaaat acaccgccgt agatgcggga gctgatagcc agagttctag
    15961 caatggcgca ctgctcggcg actccacgct gcggacgatt cagacgcagt tgaagtcgat
    16021 gctgagtaat accgtcagtt cttccagcta taaaacgctg gcgcagattg gtatcacgac
    16081 cgatcccagc gatggcaaac tggaactgga tgccgacaaa ctcaccgctg cactgaaaaa
    16141 agatgccagc ggcgtaggtg cattgattgt tggcgatggt aaaaaaaccg gcatcacaac
    16201 caccatcggc agcaacctga ccagttggct ttcgacaacg ggcattatta aagccgctac
    16261 cgatggcgtt agtaaaaccc tgaataagtt aactaaagac tacaacgccg ccagcgatcg
    16321 cattgatgcg caggtcgcgc gctacaaaga acaatttacc caactggacg ttttaatgac
    16381 ctcgttaaac agcaccagca gctacttaac gcagcagttc gaaaacaaca gtaattccaa
    16441 gtaatcatta ttcatcggga gacaggtcat gtacgcggca aaaggcaccc aggcctatgc
    16501 acaaattggc gtcgaaagca ccgtaatgag cgccagccaa cagcagttgg tcaccatgct
    16561 atttgatgga gtgctgagcg cactggttag agcgcgactg tttatgcagg acaacaatca
    16621 gcaaggcaaa ggcgtctctt tgtcaaaagc gatcaacatc attgagaacg gactgcgggt
    16681 gagtcttgat gaagagagca aagacgaatt aacccaaaac ttaattgctc tttatagcta
    16741 tatggtcagg cgtttgctgc aagccaattt acgcaacgat gtctcagcag tcgaagaggt
    16801 ggaagcatta atgcgtaata ttgccgatgc ctggaaagag tctttactct ccccttcttc
    16861 gattcaggac ccagtctgat gaacaatgca ccgcatttgt atttcgcctg gcaacaactc
    16921 gtcgaaaaaa gccagctcat gttacgcctg gcaacggaag aacaatggga cgaactcatc
    16981 gctagcgaaa tggcgtatgt gaatgcggtg caggagatcg cacacctgac tgaagaggtt
    17041 gccccgtcca ctacgatgca ggagcagctc cgcccgatgc tgcacctgat tctcgacaac
    17101 gaaagcaagg taaagcagtt attacagatt cggatggatg aactggcgaa actggtcggt
    17161 cagtcatcgg tgcaaaaatc ggtgttaagt gcctatggcg atcagggcgg ctttgtgctg
    17221 gctccgcagg ataacctctt ttgaatctta atgagccgat ggctcgcgaa taatccgatt
    17281 acggctacgc ttctaatatt ccccttgaat ggagtcgaag aatgcgtaat cccacgctgt
    17341 tacaatgttt tcactggtat tacccggaag gcggtaagct ctggcctgaa ctggccgagc
    17401 gcgcagacgg ttttaatgat attggtatca atatggtctg gttgccgccc gcctataaag
    17461 gcgcatcggg cggctattcg gtcggctacg actcctatga tttatttgat ctaggtgagt
    17521 ttgatcagaa aggcagcatc cctactaaat atggtgataa agcacaactg ctggccgcca
    17581 ttgacgccct gaaacgtaat gacattgcgg tgctgctgga cgtggtagtc aaccacaaaa
    17641 tgggcgcgga tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgat gaccgtacgc
    17701 aaattgacga agaaatcatt gagtgtgaag gctggacgcg ttacaccttc cccgctcgtg
    17761 ccgggcaata ctcgcagttt atctgggatt tcaaatgttt tagcggtatc gaccatatcg
    17821 aacaccctga tgaagatggc atttttaaaa ttgttaacga ctataccgga gaaggctgga
    17881 acgatcaggt tgatgatgaa ctcggtaatt tcgattatct gatgggtgag aatatcgatt
    17941 ttcgcaatca tgccgtgacg gaagagatta aatactgggc gcgctgggtg atggaacaaa
    18001 cgcaatgcga cggctttcgt cttgatgcgg tcaaacatat tccggcatgg ttttataaag
    18061 agtggatcga acacgtacag gaagttgcgc caaagccgct gtttattgtg gctgaatgct
    18121 ggtcgcatga agtcgataaa ctgcaaacgt atattgatca ggtggaaggc aaaaccatgc
    18181 tgtttgatgc gccgctgcag atgaaattcc atgaagcatc gcgcatgggt cgcgactacg
    18241 acatgacgca gatcttcact gggacgctgg tggaagccga tccttttcac gccgtaaccc
    18301 tcgttgccaa ccacgacacc caaccgttgc aagccctcga agcaccggtc gaaccgtggt
    18361 ttaaaccgct ggcgtatgcc ttaattttgt tgcgggaaaa tggcgttcct tcggtgttct
    18421 atccggacct ctacggtgca cattacgaag atgtcggtgg tgacgggcaa acctatccga
    18481 tagatatgcc aataatcgaa cagcttgatg agttaattct cgcccgtcag cgtttcgccc
    18541 acggtgtaca gacattattt ttcgaccatc cgaactgcat tgcctttagc cgcagtggca
    18601 ccgacgaata tcccggctgc gtggtggtca tgtcgaacgg agatgatggc gaaaaaacca
    18661 ttcatctggg agagaattac ggcaataaaa cctggcgtga ttttctgggg aaccggcaag
    18721 agagcgtagt gacagacgaa aacggcgaag cgaccttctt ttgcaacggc ggcagcgtca
    18781 gcgtgtgggt tatcgaagag gtgatttaaa tccatccccg gcggcaagcc ggggagattt
    18841 cattacggca gtggcgtcgg taacggcgct ttatccagcg cggcggcaca ctctgccgtc
    18901 gggcgatcca cacgctccat cgtcatacca tcgtactcaa tggtgttacc gtcacgctca
    18961 acctcgtaca gctcacgttt tacggtaacg ttagtgagat catcagacat caatgtcaac
    19021 ttacccggca cggcaatccc ccgctgccac tgacggcaat ccaaggtatc gccctcttta
    19081 gtgacaatca ggctgcctat cgcttccgga ctgaccagcg cacgttgcgg ccctttggtc
    19141 tgccagtagc ccgccagcca gtccggcgcg ggggttttca cgacattgtt atagttttcg
    19201 acttcggcac agcccgccag caacatgagc gcgcctgcaa ttgcgagttt tttcatcatt
    19261 cgtcctgcat gagaagaaaa gaggattgtg gcattaaagc tctgatgtcg ccagcctttc
    19321 caggttcaga gagaaatttg atctaactat tatttttgtg aattattacc ccgaaaataa
    19381 atttgcatgg tgtatgattc gcggctcttg tttcaaacct tctgagttca gaggctacat
    19441 tcatgtcatg gcagcaattc aaacacgcct ggttgattaa attctgggca cccatccctg
    19501 cggtcatcgc ggcgggtatt ctctccactt actattttgg cattactggc accttttggg
    19561 ctgtcacggg tgaatttacc cgttggggcg gtcagctcct gcagctgttt ggcgtccatg
    19621 ctgaagagtg gggttatttt aaaattatcc atctggaagg atcgccatta acccggatcg
    19681 acggcatgat gatcctcggt atgtttggcg gctgctttgc tgctgcgctg tgggccaaca
    19741 atgtcaaact acgcatgcca cgcagccgta tccgcattat gcaggccatc attggcggca
    19801 ttatcgccgg ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg ttctttaccg
    19861 gcattcctca gttctcgctg catgcctggt tctttgccat cgccactgcc attggttcat
    19921 ggtttggcgc gcgctttacc ctgctgccca tcttccgtat tcccgtgaaa atgcagaaag
    19981 tttctgccgc ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt tttcgtctcg
    20041 ggatgctggt ctttttcggc atgctgggct gggcgctgct cacggcgatg aaccaaccaa
    20101 aactggggct ggcaatgctg tttggcgtcg gctttggttt actgattgaa cgtgcgcaaa
    20161 tctgctttac ttcggcgttc cgcgatatgt ggatcaccgg acgtacccat atggcgaaag
    20221 caatcattat cggtatggcg gtgagtgcca tcgggatctt cagttacgta cagttaggcg
    20281 ttgaacctaa aatcatgtgg gcgggaccaa atgcggtaat tggtggttta ctttttggtt
    20341 ttggcatcgt gctggcaggc ggctgcgaaa ccggctggat gtaccgcgcg gtagaaggcc
    20401 aggtgcacta ctggtgggtc ggtctgggca acgtgatcgg ctcaacgatt ctggcgtatt
    20461 actgggatga tttcgctccg gcgctggcca ccgactggga caaaatcaac ctgctgaaaa
    20521 cttttggtcc gatgggaggc ctgctggtga catatttgct gctgtttgct gcgctgatgt
    20581 tgattatcgg ctgggaaaaa cgcttcttcc gccgtgaggc accgcagact gctaaggaga
    20641 tcgcatgaaa aatatcgttc ctgattaccg ccttgatatg gtgggtgaac cctgccctta
    20701 tccggcagtc gcaacccttg aggcgatgcc gcaattaaaa aaaggtgaga tcctggaagt
    20761 ggtgagcgac tgtccgcagt cgatcaataa tattccactg gatgcgcgta atcacggcta
    20821 cacggtgctg gatattcagc aagacgggcc gaccattcgt tatttaattc agaagtaatc
    20881 cctcattccc gctgtggccc acagcgggaa catcgttacc tctgacacat agattttacc
    20941 cgccccctct cctattcttt tcagtatgat ctcaggaggt tgctatgtgt ggacgctttg
    21001 cccaatccca aacgcgtgag gattaccttg cgcttctcgc ggaagatatt gaacgcgata
    21061 ttccctacga tcccgaaccc attggcagat acaatgtcgc gccgggaacc aaagttcttc
    21121 tgctcagtga acgtgatgaa caccttcatc tggatccggt tttctgggga tatgctcccg
    21181 gatggtggga taaaccgccg ctgattaacg cccgcgtaga aactgcctca accagtcgca
    21241 tgtttaaacc gctctggcaa catggtcggg caatctgttt tgccgatggc tggtttgagt
    21301 ggaaaaaaga aggcgacaaa aaaagcctta ttttatctat cgcgctgacg gacaacctgt
    21361 ttttatggcc gcgataggta gcataccgtt tgagcgtggt gacgaagccg aaggattttt
    21421 gatcgtcact gcggcggcag atcaaggtct ggtagatatt catgaccgcc gcccgctggt
    21481 tctgtcgcca gaagctgcac gggaatggat gcggcaagac attggtggta aagaagcctc
    21541 agaaatcgct accagaagct gtgtgccagc aaaccagttc acctggcacc ctgtgtcgcg
    21601 cacggtgggt aatgtcaaaa accagggcgc ggagttaatt caacctgttt gttgacatcc
    21661 tccacgccct gaatgacgag gacccctgct acgttcaggc tgtcgcctga atcatttcgg
    21721 tgggttcctg cttcaacgag cggcctgact gcaccatctc tccacaggca agaacggcgt
    21781 gccccgccgc taaaatgtta cgagctccat ttacatcggc gttcgctgta tatccacata
    21841 cctggcatct gaatttactt tgtgacaggc gattttcttt cgctgtatga ccacagcacg
    21901 cgcaacgctg gcttgtgtac gctggcggaa cagcaagcac ctgaccgcca cgccagagct
    21961 gcttgtactc aagctggcgg cgcatttcat accagccctg atccagtatc gaacggttta
    22021 aacctgattt tgcccggaca ttgcgccccg gctggctgac cgtacccgct gctgactttg
    22081 acatgttgct gaccttcaaa tcctcaatga ctatcattac gtggtttttg ctgacggtcg
    22141 ttgtgacttt gtgaaggtag tccctgcgga tatttgcgat acaggaatgc agtcgctgta
    22201 ttttgcgttt ctgcttctgc cagttgttgc tgaatttgac cttgcggctt aactgtcgct
    22261 gaagtctcgc cagcgtcttc tggtttttct gaaaactgtt tacaggctca aagactgtgc
    22321 catctgacag cgaggcaagt ttagccacgc cagcatccag cccgaccatt gatgctgaag
    22381 ggtgaaccgg agttgatact tcactttctg cctgaatact gatgtaccac ttaccgcagg
    22441 actggctgac agtgacattt ttcacaacac ccgtgacctg acggctgttc cggtagcgca
    22501 tccagccaag ttttggcaga aaaatacggc tgttttcctg agcgagctta acgccctgcg
    22561 gatagcggaa tgcatcattc tgtcaccgct ttttgaatca ggggaacgcc gagcgcttct
    22621 ggaaaaagtt tttgcaggcc tgctcagggt ctttcagtga ctgttgcaat ggctgtgacg
    22681 gagcatcttt aatccattgc gtttcagtgg catttttcca ctcaaccaat caggaagcca
    22741 ttttcgcgta agggatgtct ttgttaccgg ccttatgatt ctcattctga cgtgccagag
    22801 tacgactgaa aacgaaacga catgcgcctg cgaagcgcct catttcacgc gcctgttgac
    22861 caccgggtct taaccggaat ttgaatacct gtagtcgttt cacagggcaa attatacttt
    22921 ggtctgtgaa aaaaactgat atccgacgcg gaaggcactg tgttttcctg atgcatgctc
    22981 accgggtctt tgttaccaag taccgacgcc agatttttga ccatgacgcg acagaaaaac
    23041 tacgcactta cttttcaaat gtatgtgctt attttgaagc tgaactggtt gaaatggatg
    23101 gcgaaccaga tcacgtccat ctgttaatta actatccccc caaactggcg atatccagtc
    23161 tggtaaacaa acagcctcaa aggcgtatcg ggtaggttac tgcgacggga tcgaccagat
    23221 attgctgcca ggtattaccg caaagatgtt ttgtggcgtc ccggctattt tgccagtagc
    23281 tgcggaggtg cgccaatatc cgtcatccgc caatacattg aacaacagca aacacctggt
    23341 cagatgaaaa aacgagcctt atatccccgc cctgaaggac gggcttttac ggtgcactgg
    23401 ataagcctgg agattatcta cacctgcatg gacatcattg tactatgccc cgtacatgct
    23461 ccatcacatc ctcaacatca aagtattaga gccttgcaaa ccattatgcg tctcagtttg
    23521 tctcatctat cttacgcaaa aaaaatagaa ctaatatcaa gcg
//
LOCUS       DAZPEU010000049        23445 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPEU010000049 DAZPEU010000000
VERSION     DAZPEU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.049"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHU2L_001730"
     CDS             <1..246
                     /locus_tag="ACHU2L_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1356612.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHU2L_001731"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHU2L_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR1356613.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHU2L_001732"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHU2L_001732"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1356614.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHU2L_001733"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHU2L_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR1356615.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHU2L_001734"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHU2L_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR1356616.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHU2L_001735"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHU2L_001735"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR1356617.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACHU2L_001736"
     CDS             complement(4442..5650)
                     /locus_tag="ACHU2L_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1356618.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHU2L_001737"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHU2L_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR1356619.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACHU2L_001738"
     CDS             complement(6445..8130)
                     /locus_tag="ACHU2L_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR1356620.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACHU2L_001739"
     CDS             8400..8777
                     /locus_tag="ACHU2L_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR1356621.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHU2L_001740"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHU2L_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR1356622.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACHU2L_001741"
     CDS             9224..9511
                     /locus_tag="ACHU2L_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR1356623.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHU2L_001742"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHU2L_001742"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1356624.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACHU2L_001743"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACHU2L_001743"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR1356625.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACHU2L_001744"
     CDS             11268..11744
                     /locus_tag="ACHU2L_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR1356626.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACHU2L_001745"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACHU2L_001745"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR1356627.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14469
                     /gene="potG"
                     /locus_tag="ACHU2L_001746"
     CDS             13303..14469
                     /gene="potG"
                     /locus_tag="ACHU2L_001746"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR1356628.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLR
                     PINMMFQSYALFPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQ
                     LSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVT
                     HDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQE
                     DGLVLDSPGLVHPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHI
                     AYLGDLSVYHVRLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14479..15432
                     /gene="potH"
                     /locus_tag="ACHU2L_001747"
     CDS             14479..15432
                     /gene="potH"
                     /locus_tag="ACHU2L_001747"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR1356629.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15429..16274
                     /gene="potI"
                     /locus_tag="ACHU2L_001748"
     CDS             15429..16274
                     /gene="potI"
                     /locus_tag="ACHU2L_001748"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR1356630.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16334..16822
                     /locus_tag="ACHU2L_001749"
     CDS             16334..16822
                     /locus_tag="ACHU2L_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR1356631.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16863..17990
                     /gene="rlmC"
                     /locus_tag="ACHU2L_001750"
     CDS             16863..17990
                     /gene="rlmC"
                     /locus_tag="ACHU2L_001750"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR1356632.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18165..18896)
                     /gene="artJ"
                     /locus_tag="ACHU2L_001751"
     CDS             complement(18165..18896)
                     /gene="artJ"
                     /locus_tag="ACHU2L_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR1356633.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19188..19856)
                     /gene="artM"
                     /locus_tag="ACHU2L_001752"
     CDS             complement(19188..19856)
                     /gene="artM"
                     /locus_tag="ACHU2L_001752"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR1356634.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19856..20572)
                     /gene="artQ"
                     /locus_tag="ACHU2L_001753"
     CDS             complement(19856..20572)
                     /gene="artQ"
                     /locus_tag="ACHU2L_001753"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR1356635.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20579..21310)
                     /gene="artI"
                     /locus_tag="ACHU2L_001754"
     CDS             complement(20579..21310)
                     /gene="artI"
                     /locus_tag="ACHU2L_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR1356636.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21328..22056)
                     /gene="artP"
                     /locus_tag="ACHU2L_001755"
     CDS             complement(21328..22056)
                     /gene="artP"
                     /locus_tag="ACHU2L_001755"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR1356637.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22274..22789)
                     /locus_tag="ACHU2L_001756"
     CDS             complement(22274..22789)
                     /locus_tag="ACHU2L_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1356638.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         5773 a         5780 c         6045 g         5847 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccctagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctggg cgcatccggc
    13501 tgtggcaagt ccacgctgct gcgtatgctg gcaggtttcg aacaaccttc tgccggacag
    13561 ataatgcttg atggcgtcga tttgtcacag gttccgcctt acctgcgccc catcaatatg
    13621 atgtttcagt cttacgcgct gtttccgcat atgaccgtgg aacagaacat cgcttttggc
    13681 ctgaaacagg acaaactacc gaaagcggaa atttccagtc gggtcaatga gatgctcggc
    13741 ctggtgcaca tgcaggagtt cgccaaacgt aaaccgcatc agctttctgg tggtcagcga
    13801 cagcgtgtgg ccctggcccg aagccttgcc aagcgcccga aactattact gctcgacgag
    13861 ccgatgggcg cgctggataa aaagctgcgt gaccggatgc agcttgaagt ggtggatatt
    13921 ctggagcgcg tcggtgtgac ttgtgtgatg gtcacccacg atcaggaaga ggcgatgacc
    13981 atggcggggc gcatcgccat tatgaatcgt gggaaatttg tccagattgg cgaaccggaa
    14041 gagatctacg agcatccgac tacccgctat agcgccgaat ttattggctc ggtaaacgtc
    14101 tttgaaggcg tactcaaaga gcgtcaggaa gatggcttgg tgcttgattc gccggggctg
    14161 gtgcatccac tgaaagtcga cgcggatgcc tcggtggtcg ataacgtgcc ggtacatgtg
    14221 gcgctgcgcc cggaaaaaat catgctttgc gaagagccgc ccgccaatgg ttgtaacttc
    14281 gcggtggggg aggtgataca cattgcctat ctcggcgatc tttcggtgta tcacgttcgt
    14341 ctgaaaagtg ggcagatgat tagtgcccag ctacaaaacg cccatcgtca tcgtaaaggg
    14401 ttaccgacct ggggcgacga agtgcgtttg tgctgggaag tggacagctg tgtggtgctg
    14461 acggtttaag gagcaaagat gaatacactt gaacctgctg cccagtcgaa accgccgggc
    14521 ggatttaagc tgtggttgtc gcagctgcaa atgaagcatg ggcgcaaact ggtcattgcg
    14581 ttgccatata tctggttgat cttgctgttt ctgctgccat ttctgattgt ctttaaaata
    14641 agcctggcgg agatggcgcg cgctattcca ccttataccg aactgatgga gtgggctgac
    14701 gggcaactgt ccatcactct taatctcggt aattttctgc aactgaccga cgatccgctc
    14761 tatttcgatg cttatctcca gtcgctacag gtggctgcga tttcgacatt ttgctgttta
    14821 ctgatcggct atccgctggc gtgggcggtg gcgcacagta agccttcgac ccgtaatatt
    14881 ttattactac tggtgatcct gccgtcgtgg acctcgtttc tgatccgcgt ttatgcttgg
    14941 atgggaatat taaaaaacaa cggtgtgctg aataattttc tgctgtggct gggggttatc
    15001 gatcaaccgc tgaccattct tcataccaat ctggcagttt atattggcat tgtttacgcc
    15061 tacgtgccgt ttatggtgct gccgatttat accgcgttga ttcgtattga ttattcgctg
    15121 gtggaagcag cgctggatct cggtgcacga ccgctgaaaa cgttctttac tgtgatcgtg
    15181 ccgctgacta aaggtgggat tattgccgga tcgatgctgg tgtttatccc ggctgtgggc
    15241 gagtttgtga tcccggaact gctcggtggc ccggacagca tcatgatcgg gcgcgtgcta
    15301 tggcaagagt ttttcaataa ccgcgactgg ccggtggcct cggcggtagc gatcatcatg
    15361 ttgctgctgc taattgtgcc gataatgtgg ttccacaaac accagcaaaa aagcgtggga
    15421 gaacacggat gaataattta ccggtagttc gttcgccctg gcggattgtg attttgctgc
    15481 tgggctttac ctttctctac gcgccaatgc tgatgctggt tatctattcg tttaacagct
    15541 cgaagctggt gacggtgtgg ggcggctggt caacgcgctg gtatggtgag ttattgcgcg
    15601 atgacgcgat gatgagtgcg gttggttcaa gcctgacaat tgctgcctgt gcggcaacgg
    15661 cggcggcgat cctcgggact attgcggcgg tggtgctggt gcgctttggc aggtttcgcg
    15721 gatcaaatgg ctttgccttt atgatcaccg cgccgctagt aatgccagat gtcatcacgg
    15781 gcttgtcgct gttgttatta ttcgtcgcgc ttgctcatgc cattggctgg cctgcggacc
    15841 gcggtatgct taccatctgg ctggcgcatg tcactttctg tacggcttat gtggcggtcg
    15901 ttatttcgtc gcgtctgcgg gaactggatc gctcgataga agaagccgcg atggatctcg
    15961 gtgcgacgcc gctgaaagtg tttttcgtca ttacgctacc gatgatcatg cccgcgatca
    16021 tttctggctg gttactggct tttactttgt cgcttgatga tctggtgatc gccagctttg
    16081 tttccgggcc gggagccacc acgttaccga tgctggtctt ttccagcgtg cggatggggg
    16141 tgaatccgga aatcaacgcc ctggcaacat tgatcctcgg cgcggtcgga attgtcggat
    16201 ttatcgcctg gtatctgatg gcgcgcgcag aaaaacagcg gatacgcgac atccaacgtg
    16261 caagacgtgg ctgaagacac taaaatttgc caacctggct acataatgcc gcgcatgtcg
    16321 cggcattgtt ttcatggaag acgaaacgtt gggatttttt aagaaaacat cttcatctca
    16381 tgctcgcctg aatgtgcctg cgctggtgca ggtggcggcg ctcgccatta ttatgatccg
    16441 tggcctcgac gtgctgatga ttttcaatac gctgggcgtg cgcggtattg gcgagttcat
    16501 tcatcgcagc gtacaaacct ggagtttaac gctggtcttt ttaagcagtc tggtgctggt
    16561 tttcattgag atctggtgtg cgttttcact ggtgaaaggg cgtcgctggg cgcgctggct
    16621 atatctgctg acacaaatca ccgccgcaag ttacttgtgg gcggcttcgc tggggtacgg
    16681 ttatccggag ctgttcagca ttcccggtga atcaaaacgt gaaatcttcc atagcctgat
    16741 gctgcagaag ctgccggata tgctcatcct gatgctgctg ttcgttccct cgaccagtcg
    16801 gcggttcttc cagttgcaat aatgtgtata atcgtcgccc ctgatgatgt gaaggtcaat
    16861 gtatgcagtg cgcactttac gacgcgggtc gctgtcgttc ctgtcagtgg ataacgcagc
    16921 cgattccaga gcaactctcc gctaaaaccg ccgatcttaa aaatctgctc gccgattttc
    16981 cggttgagga atggtgcgcg ccggtgtcag gcccggaaca agggtttcgt aataaagcca
    17041 aaatggtggt gagtggtagc gttgaaaaaa cactgctcgg tatgctgcat cgcgatggca
    17101 cgccggaaga cctttgtgac tgcccgcttt atccagcctc atttgcgccc gtttttgcgg
    17161 cgctaaaacc gtttatcgcc cgagcggggt taacgcccta caacgtggcg cgtaagcgtg
    17221 gcgaactgaa atacattctg ctgactgaaa gccagagtga tggcggcatg atgctgcgct
    17281 ttgtactgcg ttctgatacc aagctggcgc aactgcgtaa ggcgctgccg tggttacacg
    17341 aacaactacc gcagctgaaa gttattaccg tcaatattca gccggtacat atggcgatta
    17401 tggaagggga gacggagatc tacctgaccg aacaacaggc actggcggag cgttttaatg
    17461 acgtaccgct gtggatccgt ccgcaaagtt tcttccagac taatccggcg gtcgccagcc
    17521 agttgtacgc caccgcgcgc gactgggtac gacagctgcc ggttaaacat atgtgggatc
    17581 tgttctgcgg tgtggggggc tttggtttac actgcgcgac gcctgacatg cagttaaccg
    17641 ggatcgaaat tgcgccagag gccattgcct gtgcaaagca gtcagccgct gaactgggct
    17701 taactcgttt acaatttcag gcgctggact ccactcagtt tgccactgct cagggggagg
    17761 tgccggagct ggtgctggtt aacccgccgc gccgcggcat tggtaaaccg ctgtgtgatt
    17821 atctttcaac gatggcaccg cgttttatta tctactccag ctgtaacgcc caaaccatgg
    17881 cgaaagatat ccgcgaactg ccaggttacc gcatcgaacg ggtacagctt tttgatatgt
    17941 tcccgcacac cgcgcactat gaagtgttga cgctgctggt gaagcaataa aaaagccaca
    18001 ggtgcggctt cagattgctg atcaggtgcg ctttgttcat gccggatgcg gcgtgaacgc
    18061 cttatccggc ctacaaaatc atgctaaatc aatacattgc agagttgatg tgggccggat
    18121 aagcgcagcg catcaggcaa tcttactttt gtcatcagtc tcaattactg cggaaaccac
    18181 tggtcattga ttttctgata tgtaccgtca gctttaattg ctgccagcgc gttattcagt
    18241 ttttccagca gagctttgtt atccggacgt acagcgatac ccaggccggt gccgaagtat
    18301 tgcggatcgg tcactttttc agtcgcaacg cccagttgcg gattggtttt cagccattcg
    18361 tttaccaccg cggtgtcacc aaatacccca tcaatacgac catttttcag atcgataaag
    18421 gcattctgat aactgtcata ggagacagtt ttcacttccg ggtgctgatc ctgaatatat
    18481 ttctggtgcg tcgtaccgtt ttccatccca atgcgtttgc ctttcaggtc ggcaaacgtt
    18541 ttgtaggtat cttttttgga aatcacgacg gctgagtttt catagtaggg tgtggtaaac
    18601 gaaacctgct tgctacgctc cggggtgata tccataccgg agattacggc gtcatatttt
    18661 ctgaatttca gtgacgggat caggctgtcg aacgcgtgat tagtaaaagt acattctgcc
    18721 tgcatttgtt tgcacaaggc ttttgccaga tcgatatcaa agccgacaat ctcattatta
    18781 gcacctatag attcaaaggg tggataggtg gctgaaacgc caaaattgat tttctctgcg
    18841 gcagaagcac cgaaagtaaa ggaagcaagt aaagcggcaa gaactaactt tttcatgatg
    18901 gaactcccgt ctgtcaatct tatgattttt ggccgtgtct gcggcatggg ataacaatgc
    18961 catcaagtga atttatatgc aataaacatg attaaataat ttaaatgaaa taaaaaaaga
    19021 cggataactt agtgagttgt ccgtcttcat tataagaatt tatgcactat gtaggccgga
    19081 taaggcgtcc acgccgcatc cggcacaggc accgtgctga tgtctgatgc gacgctggcg
    19141 cgtcttatca gacctacaaa cccccccggc gaatgtacgc agctacatta attccgccgt
    19201 tcgaatgcca gtgctttgcg ctcgatcaga cgcatcatca gcgtcagcag gccgttgacg
    19261 accaagtaaa taatccctgc cgcaccgaac accattacat cgtaggtgcg tccgtacaac
    19321 aactggctgt atcccatcac ttccatcagc gtaatggtgt aagccagaga ggtacttttg
    19381 aacaccagca ccacttcgtt ggaataagaa gagagcgagc gtttaaaggc atacggcagc
    19441 aggatcgcca gcgtatcttt tttgctcatt cccagggcgc tacaggactg ccactgacct
    19501 tctgggatcg cacgaattgc accgtaaaac agctgcgtgg tatacgccgc actgttcagc
    19561 gataacgcaa tcagcgcaca taaccacggt tctgacaaca aatgccacag tgccggatac
    19621 tcctgcaacg tcggaaactg gcccggcccg taataaatca ggaagatctg caccagcagc
    19681 ggcgtaccgg taaacagcgt gatataaccc cgcaccagcc acaccagcac cggcgttttc
    19741 agcgtcagga tgatggtaaa aatcaatgcc agaatcagtg ccacaatcag cgaggcaacg
    19801 gttagcgtca ggctggtgtg cagccctttc atcagctcgg gtaaatactc aaacattagc
    19861 tgggcctccg ctcaaaacgt gtcgcgcgca ggtcaatgcg tttgagaatg tactgactga
    19921 gcagggtgat caccaggtaa atcgccgccg ccacaatgta ccaggtaaat ggttcctggg
    19981 tacgagtagc aatgcttttg gtttgtaaca ttaaatcatt cacactaatc aaactgacca
    20041 gcgcggtatc tttcagcagc accagccact ggttaccgag gccaggcagc gcatgacgcc
    20101 acatctgcgg catcaccaga cggaaaaaga tagccgattt cgacagcccc agcgcctgac
    20161 cagattccca ctggcccacc ggtacagctt tcaacgcgcc gcgcagcgtt tgcgaggcat
    20221 aggcggcata cagcagtgac agagcgatga caccacaaag gaacggactc acgtcgaagt
    20281 tctcaatgtc catctgcacc gggatctgca cgaacccaag attgatagtg aagccatccg
    20341 aaagcgtcag cagtaactgc gaggagccaa aatagataaa cagcactacc agaatttctg
    20401 gcaggccacg cagaatggtt accagcgctg aacctgccca cgcgacagga cgccatttcg
    20461 ctgactccca taccgcaaag aacatcgcca gcgccagccc gacaatcaat gcacaaacgg
    20521 caaggccgac ggtcatcccg gcggcgcttg ctaaaggaaa aaattcattc atcaggaatt
    20581 acttctggaa ccatttgttg tagatggttt cgtaagtgcc atctttcttc actttttcca
    20641 gcgcagtgtt gagtttctgc tgcagttcag tgtttccctg acgtaccgcg atgccgaggc
    20701 cagtaccgaa ataatcttta tcggtcactt tatcgcccac tgccgccagt tttgggttat
    20761 ctttcagcca ctcagtgacc actgcggtgt caccaaatac gctgtcgata cgcccgtttt
    20821 gcagatccag ttttgcgttc tggtagctgt catacggaac ggtggtgatt tccgggtgct
    20881 tatccataat gaacttctgg tgtgtcgtcc cgttctgaac gccgactttt ttgcctttca
    20941 gctgatcaac actggtgtat ttgccttgct gacccacaaa cagggcagag ttgtcatagt
    21001 acggggtagt aaacagtacc tgcttttcac gctccggagt gatatccatg cccgccatca
    21061 ctgcttctac gcgacggaat ttcaggcttg ggatcaggct gtcaaacgcc tggttagaga
    21121 aagtacaggt tgcatcaatc tctttacaca gtgcctgcgc caggtcgacg tcaaaaccaa
    21181 cgatctggtt gtttgcatca atcgattcaa acggaggata gggggcttcg gtagcaaaac
    21241 gaatggtttc ggcagctgtg gcggaaagac taaaacctgc aattaacgcg gcaatcagaa
    21301 cttttttcat tgttgttatc ccgaatctta gtgagagaga tagtttttaa atgcttcggt
    21361 ttgcggctcg gtaaagcagc tcgcgtcgcc ttgttctacg atatgaccat tttccatata
    21421 caccactcgg ctggcggttt tacgcgccac ttcaacttcg tgggtgacga tcacctgggt
    21481 aatattcgtt tctgccagct cacgaatgat gctgacgatt tgtgccgtaa tttccgggtc
    21541 cagtgcggcg gtcggttcat cgaacagcag tacctgcggt tccatcatca acgcacgggc
    21601 aatagcaaca cgctgctgct gaccaccaga aagatgcagc gggtaacgat cgctataagg
    21661 tttgagacgc agacgttcca gcagtttttc tgcacgggcc agcgcctgat ctttactcaa
    21721 ccccagtaca cggcagggcg cttcaatcag gttttgctgc acggtcagat gcggccacag
    21781 gttgtattgc tgaaacacca tgccaacgtt acgacgcaaa tcgcgaatcg ctttgtcaga
    21841 gggtgttttg gtgaaatcga aatggttgcc tgcaatgttg agcgtaccgg agcgcggcat
    21901 ctcaagcaga ttgagtacac gcagcagcga gcttttaccc gcgccgctgg ggccaagtaa
    21961 caccagcgtt tcgccctgtg ggcaatccag cgtgatatcg aacagcgcct gatgcgcgcc
    22021 gtagaagcaa ttaatgccgt ttaattgaat actcattgac actcgtatac tggcagtctg
    22081 atagctattg aggtcgaaga tagtaccttt aacagaataa ttatgcaata tttatgcttt
    22141 aaaagttaaa agcaaagcgc attattcaat aaacatagca caaaataacg ggggcggtgg
    22201 tcggtgagca taaatgtcgg cattcctcac gaaatgccgg ataatttacg gggtttattg
    22261 gttgatcaag gcgttagcga ttctcgatgg actgacggag cgtacccgcc gtggcatgaa
    22321 cgctaccgcc taagtagcgc acatcgtcga tgacccaaca ctggccttcc tgaatcatta
    22381 acacttcatc ctgccagccc tggtcaccct gtttgagatc cacccgcaat ggaatgttac
    22441 gggcatcacg attagggata gtcgatgcac tggcaacgtg ggcgctgtct ggcaaggtgg
    22501 ttcgactgga gaacggatcg ctgcttaaca attcacgatg actattatcg cggctggcat
    22561 cgctaagttg ggttgccagt ttgtcactca aataggggcg cagggcggta atgtcattgc
    22621 tgcgatgttg aatgcggtag tcgtagaatt gttgagccac actgtcaggc cctccctgga
    22681 cacaggaacc ggtgcggggg ccgttatctt tataagctgg cgtgacggtg gtacaggcac
    22741 tcagcagcag tgcacatgga ataagtagag atagtgaacg gtagcgcata atcatttcct
    22801 tattagcttg ctgtctgcgt cacactacga ccaaacgcta tcagattatt taatacattg
    22861 tttttaatac aattctgaaa caatctgaac acctgattgg gtttgagtcc attgctggcg
    22921 acccactcaa ggttgctcgt agctgactta cttaaattac ccaaacaata gacagtgtaa
    22981 accatttgtg aggttgagcg tgttatatgg ctgaaatgat catttaaggt aaatatgata
    23041 gttctgaaat ggtggtataa atcctcctgt agaggttaag agcatctgca agaagccagt
    23101 ttcacattta taacgcgtga taaatgaatg cggttattgt ctaatcatta caaaattttt
    23161 cacatgcgat aagagatcct gcgattaaga tgattgccaa aattacgttg tatttttgaa
    23221 aatgagcgaa atttgataga acatttatct gctgtacgat ttaatgaact taatgataca
    23281 gcattacaaa aacaatcgaa gtttataaag atgatttctg attgaccagc cccctaccct
    23341 acaatggtga ttaattttat tactcgcgat ggagatgatg atatgaatat atcatatgtg
    23401 aatagcaaca aaacgacatc tttacctgtt gagctagatg cacta
//
LOCUS       DAZPEU010000050        22927 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPEU010000050 DAZPEU010000000
VERSION     DAZPEU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.050"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            19..573
                     /locus_tag="ACHU2L_001757"
     CDS             19..573
                     /locus_tag="ACHU2L_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1356639.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(583..2010)
                     /locus_tag="ACHU2L_001758"
     CDS             complement(583..2010)
                     /locus_tag="ACHU2L_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR1356640.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2007..2714)
                     /locus_tag="ACHU2L_001759"
     CDS             complement(2007..2714)
                     /locus_tag="ACHU2L_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1356641.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2878..3855
                     /gene="ybeQ"
                     /locus_tag="ACHU2L_001760"
     CDS             2878..3855
                     /gene="ybeQ"
                     /locus_tag="ACHU2L_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR1356642.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3925..4407)
                     /locus_tag="ACHU2L_001761"
     CDS             complement(3925..4407)
                     /locus_tag="ACHU2L_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR1356643.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4642..7224
                     /gene="leuS"
                     /locus_tag="ACHU2L_001762"
     CDS             4642..7224
                     /gene="leuS"
                     /locus_tag="ACHU2L_001762"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR1356644.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7239..7820
                     /gene="lptE"
                     /locus_tag="ACHU2L_001763"
     CDS             7239..7820
                     /gene="lptE"
                     /locus_tag="ACHU2L_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR1356645.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7820..8851
                     /gene="holA"
                     /locus_tag="ACHU2L_001764"
     CDS             7820..8851
                     /gene="holA"
                     /locus_tag="ACHU2L_001764"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR1356646.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8853..9494
                     /gene="nadD"
                     /locus_tag="ACHU2L_001765"
     CDS             8853..9494
                     /gene="nadD"
                     /locus_tag="ACHU2L_001765"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR1356647.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9518..10129
                     /locus_tag="ACHU2L_001766"
     CDS             9518..10129
                     /locus_tag="ACHU2L_001766"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR1356648.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10389..10706
                     /gene="rsfS"
                     /locus_tag="ACHU2L_001767"
     CDS             10389..10706
                     /gene="rsfS"
                     /locus_tag="ACHU2L_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR1356649.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10710..11177
                     /gene="rlmH"
                     /locus_tag="ACHU2L_001768"
     CDS             10710..11177
                     /gene="rlmH"
                     /locus_tag="ACHU2L_001768"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR1356650.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11208..13109
                     /gene="mrdA"
                     /locus_tag="ACHU2L_001769"
     CDS             11208..13109
                     /gene="mrdA"
                     /locus_tag="ACHU2L_001769"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR1356651.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13112..14224
                     /gene="mrdB"
                     /locus_tag="ACHU2L_001770"
                     /gene_synonym="rodA"
     CDS             13112..14224
                     /gene="mrdB"
                     /locus_tag="ACHU2L_001770"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR1356652.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14235..15323
                     /gene="rlpA"
                     /locus_tag="ACHU2L_001771"
     CDS             14235..15323
                     /gene="rlpA"
                     /locus_tag="ACHU2L_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR1356653.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15462..16673
                     /gene="dacA"
                     /locus_tag="ACHU2L_001772"
     CDS             15462..16673
                     /gene="dacA"
                     /locus_tag="ACHU2L_001772"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR1356654.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16784..17047
                     /gene="ybeD"
                     /locus_tag="ACHU2L_001773"
     CDS             16784..17047
                     /gene="ybeD"
                     /locus_tag="ACHU2L_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR1356655.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17148..17789
                     /gene="lipB"
                     /locus_tag="ACHU2L_001774"
     CDS             17148..17789
                     /gene="lipB"
                     /locus_tag="ACHU2L_001774"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR1356656.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18048..19001
                     /locus_tag="ACHU2L_001775"
     CDS             18048..19001
                     /locus_tag="ACHU2L_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR1356657.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            19210..20175
                     /gene="lipA"
                     /locus_tag="ACHU2L_001776"
     CDS             19210..20175
                     /gene="lipA"
                     /locus_tag="ACHU2L_001776"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR1356658.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20276..20479)
                     /gene="tatE"
                     /locus_tag="ACHU2L_001777"
     CDS             complement(20276..20479)
                     /gene="tatE"
                     /locus_tag="ACHU2L_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR1356659.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20608..21396)
                     /locus_tag="ACHU2L_001778"
     CDS             complement(20608..21396)
                     /locus_tag="ACHU2L_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR1356660.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21489..21872
                     /gene="crcB"
                     /locus_tag="ACHU2L_001779"
     CDS             21489..21872
                     /gene="crcB"
                     /locus_tag="ACHU2L_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR1356661.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(21926..22135)
                     /gene="cspE"
                     /locus_tag="ACHU2L_001780"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21926..22135)
                     /gene="cspE"
                     /locus_tag="ACHU2L_001780"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1356662.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22310..22870)
                     /gene="pagP"
                     /locus_tag="ACHU2L_001781"
     CDS             complement(22310..22870)
                     /gene="pagP"
                     /locus_tag="ACHU2L_001781"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR1356663.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5902 a         5751 c         5665 g         5609 t
ORIGIN      
        1 aaatcgaagc attcacacat gaataaaaaa ttaatgtata tattcgcaat ttttatagtt
       61 gcagcaatta cctgtattag ccaacccaag aaaacgatgt tgcgtgataa agccatggtg
      121 aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac ggcagccacg
      181 gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct gaaaggcctg
      361 ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      541 gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga agagatggag
      601 tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga tgacgcccag
      661 acagcccagc ccgccatcat caacgggcat tggctgcggt tgcggctccg gctcgacggt
      721 ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
      781 tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt cttctgtcgg
      841 ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt
      901 atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga
      961 acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg
     1021 ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca gatcacgcgc
     1081 ttcaatcatc atcaccacta atgggtaata atagacccga tgctgacggc accattgcaa
     1141 cattaaagca tccgcctggg tggagggata ctcgcgccag tagtaacaaa gttctgccag
     1201 caggctttcg ccttctccac aatagacgcg ttgtgcgtat tcataataac gcgcatcttc
     1261 attatcaggt tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg ggattcccag
     1321 gcggcttgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat
     1381 cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg
     1441 agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaactggca gatgcagaat
     1501 ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc
     1561 ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac
     1621 cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa
     1681 ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga
     1741 gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg
     1801 ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg
     1861 tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg
     1921 aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc
     1981 tatatcgagg atcttccagc aatttttcat aagtcgctca tatccttaag cgtttccggg
     2041 catgtggggt agtcagccag tggttgccag gacaaggtag ggtaatacgc atcatcactg
     2101 gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat
     2161 tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta
     2221 taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc
     2281 gactggatcc atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt
     2341 aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct
     2401 gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc aagatagtca
     2461 cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa ccgagcggca
     2521 tgaccgttct cgatcattcg gtgaattatg cgcagcaggt catcacggtc atcaattccc
     2581 catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga tgaatgcgaa
     2641 ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc
     2701 gaccccatgt ccatataatt tatccttaaa cattcattta atgaatgtaa actacatttc
     2761 gaggctcagt tatttatact tttctgacgc aacataatac gcataaaata aggcttacgc
     2821 aaaaatggaa cacataaaaa tataatatat tttatctaca accataagga aatgataatg
     2881 atttttacgt caagttgctg cgataattta tcaatagatg agattatcga acgtgctgaa
     2941 aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga tagcgcaatt
     3001 gactctccgg acgacgaaaa ggccttttac tggctgaagc tggccgctga gcaaggtcat
     3061 tgtgaagcac agtattcctt agggcaaaag tataccgagg ataaaagccg tcataaagat
     3121 aatgagcaag ccatcttctg gctgaaaaaa gctgccctac aaggccatac tttcgcttcc
     3181 aacgcccttg gctggatact ggatcgtgga gaagacccca actataaaga agcggttacc
     3241 tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct tggatggatg
     3301 tacagaaatg gcaacggagt cgcacaagac tatacgctgg catttttctg gtacaaacaa
     3361 gctgcattac aaggccatag ttacgcacaa gacaatctgg ccgatcttta taaagacgga
     3421 gaaggcgttg ctcaaaacaa gacactcgcc gcattctggt atttgaaaag cgcacagcag
     3481 ggtaatcggc acgcccagtt tcaaattgca tgggattata acgctggcga aggggtggac
     3541 caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcagga aagcgtcggc
     3601 gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa agattatcag
     3661 gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc ctggtataac
     3721 ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca ggctctcgac
     3781 ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat tcgtgagact
     3841 gaagctttac tgtagaaata aaaacaaggg ccggggaatt tcattctgcc catattctct
     3901 ttattgctgt gcgaaatcag actattacgg ctcaaacggg cgtctctgga actggtcata
     3961 accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat ggaagtgaca
     4021 tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa ccccatgatg
     4081 attgaggtcc tggaaaattt cgcgccattc aagctgcgtt ttatcggtga tgtctgccag
     4141 ctcctgccac aagctttctt taatcacccg cataaagacg ctgtcagatt cttctttcag
     4201 gctctcttca tagctcatgg cgaactcttc caggtcacgt ctgacagctc gcgtcagctc
     4261 atcgatctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg cctgttccac
     4321 cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ttcagtgacg cgaccagttc
     4381 acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa ctgactataa
     4441 taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt aaattaatgt
     4501 acgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg
     4561 tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt agccgtattg
     4621 aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat agaatccaaa
     4681 gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag
     4741 aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat gggccacgta
     4801 cgtaactaca ccatcggtga cgtgatcgcc cgttaccagc gcatgctggg caaaaacgtt
     4861 ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac
     4921 aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa
     4981 atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc ggaatactac
     5041 cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata taaaaagact
     5101 tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca agttatcgac
     5161 ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca gtggtttatc
     5221 aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga tcactggcca
     5281 gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc
     5341 ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc ggacaccttt
     5401 atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa
     5461 aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt tgccgaagct
     5521 gaaatggcga cgatggagaa aaaaggcgtc gatactggct ttaaagctgt tcacccatta
     5581 acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta cggcacgggc
     5641 gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc taaatacggc
     5701 ctgaacatca aaccggttat cctggcggct gacggctctg agccagatct ttctcagcaa
     5761 gccctgactg aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct tgaccatgaa
     5821 gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga gcgtaaagtg
     5881 aactaccgcc tgcgcgactg gggtgtttct cgccagcgtt actggggcgc gccgattccg
     5941 atggtgacgc tggaagacgg taccgtaatg ccaaccccgg acgaccagct gccggtgatc
     6001 ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga tccggagtgg
     6061 gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt
     6121 atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga aggtatgctg
     6181 gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg tatcgaacac
     6241 gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg
     6301 gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc
     6361 ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga tgctatcgtt
     6421 gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt
     6481 tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg
     6541 gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc tccggctgat
     6601 atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct gaaacgtgtc
     6661 tggaaactgg tgtacgagca cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg
     6721 ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc taaagtgacc
     6781 gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat ggagctgatg
     6841 aacaaactgg cgaaagcacc gaccgatggc gagcaggatc gcgctctgat gcaggaagca
     6901 ctgcaggccg ttgtccgtat gctcaacccg ttcaccccgc acatctgctt cacgctgtgg
     6961 caggaactca aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa
     7021 gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa
     7081 atcaccgtcc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg ccaggaacat
     7141 ctggtagcaa aatatcttga tggcgttact gtacgtaaag tgatttacgt accaggtaaa
     7201 ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg gcaacactgt
     7261 tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt gacaccacgc
     7321 aggttccttc cactatgaag gtcatgatcc tggactcagg cgatccgaac gggccattaa
     7381 gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat aaagaaacca
     7441 cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat
     7501 cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg aatgcgaccg
     7561 tgttgatccc cggccgtgat atctacccga ttagcgccaa agtcttccgt tcattcttcg
     7621 ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta aaagagatgt
     7681 acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct gcggatattc
     7741 gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct
     7801 ccaccatgct gggtaactga tgattcggtt gtacccggaa caactccgcg cgcagctcaa
     7861 tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat tgcaggaaag
     7921 ccaggacgct gttcgccagg tagctgcggc acaaggattc gaagaacacc acactttttc
     7981 cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt
     8041 tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa
     8101 tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctattga tcgtccgcgg
     8161 taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg cgaatcgcag
     8221 cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg ttgctgtgcg
     8281 cgcaaaacag ctcaacttag aactggatga cgcggcgaat caggtgctct gctactgtta
     8341 tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc tctggccaga
     8401 cggcaaactg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc
     8461 ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc atattcttca
     8521 gcaactgcgt ctggaaggca gcgagccggt tattttgttg cgcacattac aacgtgaact
     8581 gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg cgttgtttga
     8641 taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa atcgcttaag
     8701 tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca ccctcaaaca
     8761 agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt tgtgccataa
     8821 acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct ctgtttggcg
     8881 gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg gcgaatttga
     8941 ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt ccccagccgg
     9001 aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac aagccattat
     9061 ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgcg caaacactga
     9121 aagagtggcg gcaggaacaa ggaccggacg tgccgctggc gtttattatt ggtcaggatt
     9181 cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat gcacatttga
     9241 tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac cagcaatggc
     9301 tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc ggtaaaattt
     9361 atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa cgtttgcaaa
     9421 acggtgaatc gtgtgaggat ttattgccgg aaccggtatt gacttacatt aaccaacaag
     9481 gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt taattcgtca
     9541 tggcgaaaca caagcgaatg tcgatggtct gtacagcggt catgcgccca cacccctgac
     9601 cgcacgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg tttcctttga
     9661 cttggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg ttctcagtga
     9721 ccgccagctc cccgttcata tcatccctga actcaacgaa atgttttttg gcgactggga
     9781 gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa
     9841 tgactggcag catgctatcc ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt
     9901 ggaacgcttt atcgcaaggc ttagtgaata tcagcactat cagaatattt taatcgtcag
     9961 ccatcagggc gtactgagtc tgttaattgc tcgtttaatt ggcatgcctg ccgagtctat
    10021 gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc aaaaattcgc
    10081 gacgctacgc gtcctcaata gccgtgccat cggggtcgag aacgcatgac ttttctgttt
    10141 ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat tctccgcagc
    10201 cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat
    10261 ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc
    10321 gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg
    10381 gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca
    10441 aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc gactgcatga
    10501 tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac gttgtgcagg
    10561 agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga
    10621 ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt
    10681 atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtgggaacga
    10741 aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata
    10801 tgcccttcga gctgattgaa attccggccg gcaaacgcgg caagaatgcg gacatcaagc
    10861 gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca
    10921 ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag ctggaacgct
    10981 ggaagctgga tggtcgcgac gtaagtctac tgattggcgg gcctgaaggg ttgtcgcctg
    11041 cctgtaaagc ggcggctgag cagagctggt cgctgtcggc gcttaccctc ccccatccgc
    11101 tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc
    11161 cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg aaactacaga
    11221 actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct
    11281 ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg
    11341 ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg gtgcctatcg
    11401 cgcccagccg cggcattatc tacgaccgta acggtatccc tctggccctc aaccgcacta
    11461 tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg ctggacgctt
    11521 tgcgcagcgt ggtagatctg accgatgacg atattgctgc attccgaaaa gagcgcgcac
    11581 gttcacaccg tttcacctct attccggtga aaactaacct aaccgaagta caagtagctc
    11641 gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt
    11701 actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata
    11761 aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata
    11821 ttggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg
    11881 aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc
    11941 aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc
    12001 tgctggcggg tagccgcgca gctgtggttg ttaccgaacc gcgtacaggt ggggtgctgg
    12061 cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc tccagcaaag
    12121 attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc acacaggggg
    12181 tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcatta agcgccgggg
    12241 tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca gggtcggaaa
    12301 aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca agatcgctgg
    12361 aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct
    12421 ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg gcggaagaac
    12481 gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc
    12541 agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc ccaatccaga
    12601 tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg catttgctga
    12661 tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg
    12721 gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc
    12781 gccctaatgg tacggcgaat aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat
    12841 ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg
    12901 ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag
    12961 tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt acgctgatgc
    13021 gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct gcggaaaatc
    13081 cagcggttgc cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt
    13141 ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat tgctggttta
    13201 cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat
    13261 cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt
    13321 ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga
    13381 tgctttcgga gccatctcta aaggcgctca acgctggctg gacctcggta ttgttcgttt
    13441 tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct ttatcaaccg
    13501 cgacgtttgc ccgccatcgt tgaagaatac tggcatcgcg ctggtgctga tatttatgcc
    13561 cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg
    13621 tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct
    13681 ggtagcggcg ttcattccga ttctgtggtt cttcctgatg catgattacc agcgccagcg
    13741 cgtaatgatg ctcctggacc cggaatcaga cccactcggc gcgggctatc acattattca
    13801 gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc acggcactca
    13861 gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg tactggcgga
    13921 agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc tgatcatgcg
    13981 cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt
    14041 aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc
    14101 ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat
    14161 ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt
    14221 gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg
    14281 cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag cctgcggtat
    14341 gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca ctgaacgcga
    14401 cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc
    14461 gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt aacctgacgg
    14521 cctctggcga agctttcgat ccgacaaagc tgacggcggc ccatccaacg cttccgatcc
    14581 ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg
    14641 atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca gctgaccgtc
    14701 ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt
    14761 cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgcctg
    14821 cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg cagggtgaca
    14881 ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca
    14941 gtagcggttt cctcggcgct ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac
    15001 cgacgcctgc gccacagccc gttgttacag cttcgtcgac aacgcctgca acctcgcctg
    15061 caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg
    15121 gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga cagaagttcg
    15181 gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt ggcccattcg
    15241 ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt
    15301 catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat
    15361 gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag
    15421 ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc attttttccg
    15481 ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt atctctgctg
    15541 cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg
    15601 agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag aacgcagatg
    15661 tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa
    15721 tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac gcatgggcca
    15781 ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc atgcaggttc
    15841 cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca
    15901 tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac agctacgtta
    15961 acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat gctgatggtc
    16021 agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt gacgtaccga
    16081 atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc
    16141 gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc ggacacactg
    16201 acaaagcagg ttacaatctt gttgcttctg cgactgaagg ccagatgcgc ttgatttctg
    16261 cggtgatggg tggacgtact tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct
    16321 ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag ttcgcctctg
    16381 aaccggtttg gtttagtgat tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc
    16441 tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg aacagcagtg
    16501 aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc cagcttgatg
    16561 gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa ggtaacttct
    16621 tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt taaaaattga
    16681 acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa actcccgcct
    16741 tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa ccaaacttaa
    16801 cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg cgttacctga
    16861 gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact acaccccaac
    16921 ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca acgccactca
    16981 tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg tccgcatggt
    17041 tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactcccctc tcctcgctgt
    17101 gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg tatcaggata
    17161 aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccgg gctatgcatg
    17221 aattcaccga tacccgcgat aatagtaccc ttgatgaaat ctggctggtc gagcactatc
    17281 cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg ggtgatattc
    17341 cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga caacaggtga
    17401 tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc
    17461 ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat cctcgggctg
    17521 acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta cgaattcgac
    17581 gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca ccatttttac
    17641 gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa tggaaacccg
    17701 aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg ctactaaaca
    17761 atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa tttacactgg
    17821 gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta tattttcttt
    17881 atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt gattaaccta
    17941 ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat aagattattg
    18001 taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg gatagtaata
    18061 atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa atatttacaa
    18121 ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat gtacataaag
    18181 ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt cagtctattc
    18241 agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag ggtgtcactc
    18301 ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag tccattcttg
    18361 gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt gcgaccactc
    18421 cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact cactatccgc
    18481 agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt cagtttcaaa
    18541 ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat catgttctgt
    18601 tcaccgacaa tatggtgtta atttgccgca agggaaatcc actactctct ttagaagatg
    18661 acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaaggg caaaatttca
    18721 gcggtctgcg gcagagagtt caagagatgt ttccggaccg gcaaatcaat ttcaccagct
    18781 acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg attattccca
    18841 gccgttttta taacctgttt agtcgttgct ggccgctgga aaaactgcct tttccgtcct
    18901 taaatgagga gcaaatagat ttctctatcc actacaacaa attcatcctg cgtgatccca
    18961 tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt gttacagcca
    19021 gatcacagcg cagaacgata aaatggcaca atccaacaac aattttacat tttcgcgacc
    19081 gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat tcaaaaatag
    19141 ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg caactggaac
    19201 acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg cgatgccgat
    19261 aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct gctgcgcaag
    19321 ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg catcaaagcc
    19381 gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc taacctggcg
    19441 gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg tacccgccgt
    19501 tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa tgaaccagtg
    19561 aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac ctccgttgac
    19621 cgtgatgacc tgcgcgatgg cggtgcccaa cactttgcgg attgtattac tgccattcgg
    19681 gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgcgg tcgaatggat
    19741 cgtgctctgg atattctgac tgcaacgccc ccagatgtgt tcaaccataa cctggaaaac
    19801 gtaccgcgta tttaccggca ggtacggcct ggcgcagatt acaactggtc gctgaagctg
    19861 ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct gatggtgggt
    19921 ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg tcatggtgtg
    19981 acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc ggttcaacgt
    20041 tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat gggctttacc
    20101 catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca ggcgaaagga
    20161 atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa acccgctttt
    20221 tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg ccacgtcact
    20281 ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc gccgcagcat
    20341 cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct ccgcccagcg
    20401 tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc agcagtttgg
    20461 taatactaat ctcacccata gataccttct tgacatataa ccggcaacag aacgcgcctt
    20521 aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat ctgtaacaag
    20581 gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa agcgacggtt
    20641 gtttaagatg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca cttccgccat
    20701 aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat ctataatccg
    20761 gctttgcccg atatttttgt tcccgcactc ccccgccgcc accatataac aggtggtatc
    20821 cagcgcacga gcggcaagca acgttgacca gtgatgctct ttgagcggcc cacgaaccca
    20881 ggcggcagga agtaccagga tttcagctcc ctgtaaagcc tgtgccagcg ccagctctgg
    20941 aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccttccacct ccagtaacgg
    21001 agctatttca ttaccagcat caacatggcg tgattcctga atggcaaatg catcatagag
    21061 atgcagtttg gcataacggg cgacgatgtt tcctgcctga agtgccacca gcatattcca
    21121 tgcgcgcccc ggcgttgaag gaagatgaat cgtcagaatt gtcgtcatca tgttacgttt
    21181 actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg atttaaccga
    21241 tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacaa acagcgatac
    21301 gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct tttcccacac
    21361 agatgtaaca gcaaactgtc ctgcagcaac cagcattgcc acctcctgtc agagtcgtaa
    21421 aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc aaatagcagg
    21481 attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg ggaagcgtag
    21541 cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg ttggggacgc
    21601 tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg ttcagcagga
    21661 tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt ggcggtctaa
    21721 caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc cgctttggct
    21781 gggcattact gaacgttttc gtcaaccttc tggggtcttt tgccatgacc gcactggcat
    21841 tctggctgtt ttcggcctca accgtacact aaagaccaaa aaaaacccgc tgattaagcg
    21901 ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca gaagggcctt
    21961 tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt ttaaaaccat
    22021 tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc ggagtaatga
    22081 aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta gacatcaaaa
    22141 ttacctttac atgaaaaata gacacaaatg ctgtgtcggt tatcagtaca ccaattgtgg
    22201 tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat ttttgatgtt
    22261 gcttatttta gttattgatt ttaaagaagt tactaaaact tcatttgtct caaaactgaa
    22321 agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg taggtcatct
    22381 gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg agagggatgt
    22441 aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa tgaaaatttt
    22501 catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt tcccatttgt
    22561 tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt tcatcccaac
    22621 gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt ttttctttgt
    22681 cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa tgttcaggct
    22741 gttgccaggt ttgtgcaata ttttctctaa acgttgtcat cctctcatct gcgttagcaa
    22801 aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg acatatttac
    22861 tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca tagcaaagct
    22921 acaatta
//
LOCUS       DAZPEU010000051        22885 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPEU010000051 DAZPEU010000000
VERSION     DAZPEU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.051"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            134..1156
                     /locus_tag="ACHU2L_001782"
     CDS             134..1156
                     /locus_tag="ACHU2L_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR1356664.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1156..2136
                     /locus_tag="ACHU2L_001783"
     CDS             1156..2136
                     /locus_tag="ACHU2L_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR1356665.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2133..2891
                     /locus_tag="ACHU2L_001784"
     CDS             2133..2891
                     /locus_tag="ACHU2L_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1356666.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            2901..3710
                     /locus_tag="ACHU2L_001785"
     CDS             2901..3710
                     /locus_tag="ACHU2L_001785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1356667.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            3710..4564
                     /gene="modD"
                     /locus_tag="ACHU2L_001786"
     CDS             3710..4564
                     /gene="modD"
                     /locus_tag="ACHU2L_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR1356668.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            4590..6544
                     /locus_tag="ACHU2L_001787"
                     /pseudo
     CDS             4590..6544
                     /locus_tag="ACHU2L_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(6594..6848)
                     /locus_tag="ACHU2L_001788"
     CDS             complement(6594..6848)
                     /locus_tag="ACHU2L_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1356669.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7049..7783
                     /locus_tag="ACHU2L_001789"
     CDS             7049..7783
                     /locus_tag="ACHU2L_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR1356670.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7785..8396)
                     /gene="emtA"
                     /locus_tag="ACHU2L_001790"
     CDS             complement(7785..8396)
                     /gene="emtA"
                     /locus_tag="ACHU2L_001790"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR1356671.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8496..9410
                     /gene="ldcA"
                     /locus_tag="ACHU2L_001791"
     CDS             8496..9410
                     /gene="ldcA"
                     /locus_tag="ACHU2L_001791"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR1356672.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            9505..11241
                     /locus_tag="ACHU2L_001792"
     CDS             9505..11241
                     /locus_tag="ACHU2L_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR1356673.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            11852..13266
                     /locus_tag="ACHU2L_001793"
                     /pseudo
     CDS             11852..13266
                     /locus_tag="ACHU2L_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13434..14504)
                     /gene="dadX"
                     /locus_tag="ACHU2L_001794"
     CDS             complement(13434..14504)
                     /gene="dadX"
                     /locus_tag="ACHU2L_001794"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR1356674.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14514..15812)
                     /gene="dadA"
                     /locus_tag="ACHU2L_001795"
     CDS             complement(14514..15812)
                     /gene="dadA"
                     /locus_tag="ACHU2L_001795"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR1356675.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16142..17674
                     /locus_tag="ACHU2L_001796"
     CDS             16142..17674
                     /locus_tag="ACHU2L_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR1356676.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(17726..18445)
                     /gene="fadR"
                     /locus_tag="ACHU2L_001797"
     CDS             complement(17726..18445)
                     /gene="fadR"
                     /locus_tag="ACHU2L_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR1356677.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18666..20207
                     /gene="nhaB"
                     /locus_tag="ACHU2L_001798"
     CDS             18666..20207
                     /gene="nhaB"
                     /locus_tag="ACHU2L_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR1356678.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20353..20883
                     /gene="dsbB"
                     /locus_tag="ACHU2L_001799"
     CDS             20353..20883
                     /gene="dsbB"
                     /locus_tag="ACHU2L_001799"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR1356679.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(20929..22197)
                     /gene="umuC"
                     /locus_tag="ACHU2L_001800"
     CDS             complement(20929..22197)
                     /gene="umuC"
                     /locus_tag="ACHU2L_001800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1356680.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22197..22616)
                     /locus_tag="ACHU2L_001801"
     CDS             complement(22197..22616)
                     /locus_tag="ACHU2L_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1356681.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5483 a         6006 c         5723 g         5673 t
ORIGIN      
        1 gatagagagg gtcaacaggc ttgcggaagt cgtcacatct tcatgttttc aatagctaaa
       61 ctttgatatt gaacatgatt tttatcgata tatatgaaag tatatatcga ttttactttt
      121 caggatgttc accatgccca taaccagacg gacgtttgcg caggcacttg catctaccct
      181 gctcctgcag tctctaccgt ccttttcaca aacagttaat cgttttgctt cgcaatctct
      241 tcccgaagcc caaaacatta cacgcatcgt cagcgcaggc gcgcccgccg atttgctctt
      301 gctggctgtc gcgccagaaa aaatggtcgg tttttcctct tttgattttg cccgtcaggc
      361 attaattcca ttgccagagc acattcgcca gttacccagg ctgggacgac tcgccgggcg
      421 cgccagcaca ctatcgctgg aagggcttat ggcgttacat cccgatttgg tcgttgattg
      481 cggcaatacg gatgaaacct tgatctccca ggcacggcag gttagcgaac agacacaaat
      541 cccctggtta ttgcttaacg gaaaactggc gcaatcagca gaacagttaa caacgcttgg
      601 caaaacgtta ggcgaagagc accgcgccgc agaacaagcc aatctcgcca gccacttcgt
      661 tggtgaagct caggcattcg ccacctcacc cgccgctaac ctccgctttt atgctgcgcg
      721 cggtccccga gggctggaaa cgggcttaca gggttcgttg cataccgagg cggcggaatt
      781 attaggtttg cacaacgtcg cgcaaatagc cgatcgccac ggcctgacac aggtttccat
      841 ggaaaatctc ctgcgctggc agccggatat tattctggtt caggaggccg ttactgcaga
      901 ttttattcgt cgtgatcctc tctggcaggg cgtgaaagcc gttgcggaac aacgcatctt
      961 atttttaagt ggcttgccct ttggctggct cgatgccccg ccgggaatca accgtcttct
     1021 gggattacgc agacttcacg cctggctgga tcccgccatc aatcgccagt ttaaaagtga
     1081 catgcagcat tacgcccaac tgttctggca ttgttcactc agtgatgccg actatcaaaa
     1141 attggtggcg agctaatgag aatcgtaaac ggatgtattt tgctgctggc agctatcagt
     1201 atcacttttg ccgccgtaag tggggcttat catctcgata tgcagcaact gctggcgcta
     1261 attcttcgtc aagaaaatga gtctgtgcag gaacaaattg tcttctggca aatccgcgtg
     1321 ccgcgtattc ttgctgcgct gttactgggc gcagcgctgg caggagccgg aaccacctat
     1381 cagggaatgt tgcgtaatcc cttagtttca ccggatattt taggcgtctc cgccggtgcc
     1441 ggacttggtg cctgcgcggc aatcctgtgg ggattaccca ttgtttacat ccagctgtac
     1501 gcattttgcg gcgggctgat ggtggtcgcg ggcgtctggt tgattacgcg tcgggtgacc
     1561 cgccacgatc cgatcttaac cctggtgctg gtaggcattg cgttaggaac gttgtgcgga
     1621 gcggggatat cgctaattaa aacgctggcc gatccttaca cccagttacc gtcaattacc
     1681 ttctggttgc tggggggact gtccaccgtg accttacgcg atctctgcta tgctgcaccg
     1741 atcattctca taggctcctt gcccctgttt tttctccgct ggcgcatgaa tttactcacg
     1801 ctttccgacg acgaagcccg ttcgctggga ctgaacgtga cgcgtctgcg ctttggattg
     1861 attgtctgcg ccacactaat caccgccagc acggtagcaa tagcgggcat aattggttgg
     1921 atcggtctgg tcgttccgca cattgcccgc ttgttgaccg gtcataacca tcagcagctg
     1981 ttgcctatgg caatgtgtac tggcgctatt ctgctcctgc ttaccgatac gctggcgcgc
     2041 agcattggaa caacagaaat cccacttggc attctgaccg cttttgttgg cgcaccgttt
     2101 ttcctgcttt tgttgttacg cggaggtcgc cagtgacttt gcttgccgtt cgtcatgcgt
     2161 ctctgggtta ctcccgccat ccagtgctac gcgacgtgtc cttcaccctg tcgcaaggaa
     2221 caatttgttg tctgcttggc gcgaatggtt gcggcaaaac cacactcatg cgctccattc
     2281 ttggcgttat accgttatta aaaggcgaga tcctactgga tagcctctct gtgcagacac
     2341 tcagccatcg ccagcgcgca caggctattg cctgggtgcc gcaggcgcac gacggtattt
     2401 ttgctttcag tgtgctggat atggtgttga tgggcctggc ccccaccata ggcgcttttt
     2461 ccgttcccgg taagcaagaa cgcctgaaag ccacggagca actggaaaag ctgggcattt
     2521 tgcatctggc gacacgacgc tggaatacgc ttagcggcgg tgaacgtcag ctggctttaa
     2581 tcgcccgtgc actcgtccaa caaccgcgct tgttgttact ggatgagccc gcctccagcc
     2641 tcgatttcgg tcatcagatc caacttctgg acacgctggc gcagctcaaa aataacggca
     2701 tgacaatgtt aatgtcgact caccatacgc tccatgccaa cgccattgcc gacagcatta
     2761 tccaagtaga gccagatgga cgagtaacac aaggcttacc gaccgaacag ttaacaacga
     2821 acaaactggc ggccctgtat cgggtttctg ccgaccaaat tcatcatcac ctctctgcaa
     2881 ttagccgcta aggaacaatc atgctcattg atgatattga ttttgcagac ctttatctgc
     2941 agcaactcaa actggctcac cgcacagaaa aaacgcccga tcactgggat caacgggcgg
     3001 agaaaatggc ggaaaattgc gcctcgccaa cggacagcta cctgcaacaa ctgatcgcta
     3061 aaattgacct gcaaggcgct cagaccttat tcgatatggg ctgcggacca ggcaccgtat
     3121 cgctggcgct ggctgacaaa ctcactacgg tttatggcgt tgattacagt caggggatgc
     3181 tgaacgttgc cgcccgccgc gccgccgcac tgaaggctga caatgtccac tggatccagc
     3241 gcgcctggga agaggactgg agtgacctgc cacgctgcga tattgccgtg gcttcccgct
     3301 ccactctggt ggctgatatg cgccatgcaa tgagcaaact gaataatcag gcacggcttc
     3361 gcgtctatac aactcatctg gtcagcacct cgttcgtctc tccagccatt cagcgggcgg
     3421 ctggtcggga agtgatcgaa ttacccaact atatctttgc gctcaacgtc ctgtatcaga
     3481 tgggaattta tgcccacgtt gattttatcc gtggacaaaa ttgccaacag gataacagca
     3541 cctgggaacg ttttgagcag aacgtcagct ggagtcttgg cgcgcttaac gacgatgagc
     3601 gtgagcggtt atatcactgg tatcaacaac aagatgctcg tgccctcgcc cctgccagcc
     3661 gcgactgggc gctcatctgg tgggatagcg taccgcagga ggcactgtga tgattttcct
     3721 ttctcaggca caaatcgatg cgttactgct ggaagatatc cagggaggtg acctgaccac
     3781 gcgagcatta aatattggac accagcctgg ctatatagcg ttttttcacc gtcagggcgg
     3841 ctgcgtcagc ggcgtttctg tcgcgtgtaa gatgttaact acgctgggat taactattga
     3901 tgacgcggtc agcgacggtt cacaagcgaa cgcgggtcaa cggctaatcc gtgcgcaggg
     3961 taatgccgca gcacttcatc agggatggaa ggcagtccag aatgtgctgg agtggagttg
     4021 cggtgtttct gattatctcg ctcaaatgct ggcattgctt cgtgaacgtt atcctgacgg
     4081 caatatcgcc tgcacccgaa aagcaattcc gggcacccgc ttactggctt cgcaggcaat
     4141 tctggctgcc ggaggactaa ttcatcgtgc cggatgtgcg gaaaccatat tattgtttgc
     4201 caaccaccgc cattttcttc atgacaatca gggctggtca ggcgcaatcg ctcagttacg
     4261 tcgccacgca cccgagaaga aaattgttgt tgaagccgac acgccgaaag aggctatcgc
     4321 cgcgttacgc gcacaaccag acgtacttca gctcgacaaa tttagtccgc aacaggcaac
     4381 tgaaattgcg caaatagcgc cgtcgctggc tcctcactgc acgctggctc tgaccggcgg
     4441 aattaatctg acgacactca aaaattacct cgactgcggc attcgccttt ttattacctc
     4501 cgcgccttat tacgcggcac cagctgacat taaagtcagt ctgcaaccca cagcctctat
     4561 ttaattcttt gttaaacgga atttattcta tgagactcaa aaaacgttat ctctgcacag
     4621 cattatcact tgcctttacc cagcaggccg tagcggctca ggagagcgac acgctgaccg
     4681 tatggtccag tccggtatca tcgacgacga ccaccgttct cgatcaaccc accatgaagg
     4741 ccttggataa acagaatgtc gctcaggcat taagtgtcgt ccccggcgtg gtgctgcaaa
     4801 agtcaggcag ccgcaacgaa gaacaggtaa aagttcgtgg ctttgatagt cggcaggtgc
     4861 cggtctattt cgacggtgtg cccatttatg tcccctatga cggcaacctc gatctggcgc
     4921 ggattctgac caacaatctg ggggcagtcg aagtttccaa agggtattcg tcgctgcttc
     4981 atgggcctaa tcagatgggc ggagccatta atatcaccac cctgaagaag caagtctggg
     5041 atatcgccag ggatggagcc gtagtcagga caatgcctac gatatgcatg cttcatttgc
     5101 cgccagcagc gatcttgggt atttgcaagt cagcggtagc cagctaaagc aggattttct
     5161 cggcctgccg catggtgtaa ataatgatat tgcaggcaaa cacggcaaga tgattaattc
     5221 atcggctgat gataaacgcg gcattgtgaa gctcggtttt acaccgcgcg aaaacgatga
     5281 atacacgctg acttacatta agcaggatgg tgaaaaagat aacccgccat acagcggaaa
     5341 ttgtggtcaa aaatcacgct actggcagtg gccagagtat gacaaagaaa gtttttatta
     5401 tcagggagcg acccaactaa acgaccgttt taccctgaaa agtcggctgt atcgcgacac
     5461 ctttgaaaat acgctgatga tgtacaactc gctggctgat ttgaaaaata aaaaaggcag
     5521 ctacagccat tattccgatt acagcgacgg tgccggatta caactggcag ccgatgtgcg
     5581 tgaaaacgat ctgctgtcgt ttgccgttaa ctggaaagat gacgtacacc gggaaaaagg
     5641 tgcgccgcac gccgcttacg atcgctatga agatcgtacc tggtcgctcg ccagtgaata
     5701 tcaatgggct gctgctgata atgtcgatgt cgtggcggga atcagctatg actggcgcga
     5761 tagcgtagaa gcgaaaaaac acgagaaaga tggcagcatc acccattatg acgacaacaa
     5821 tcagtcagct tttaactggc aggtgatggg gaaataccac tttgccaatg aagacactct
     5881 ggcgctttcg tactatgacc gcacacgctt tccgacgctg aaagaacgct ataccacatc
     5941 caaacctgcg tataaccaga tagcgattgt taacccgcag ctcaagccgg aacgcgctcg
     6001 cggggtggat ttaacctgga atggtgcctt cacgcgcgac tggggatttg aggtcagcgt
     6061 ttactataac cgggtgagtg atgccatcct ctcgcacaat atcaatgccg ataccattca
     6121 aaatcagaac agcggcacgg tggattacag cggtctggat gccggtatta aggggaaaat
     6181 cagcaatata ctggatgtag gattgagcta cgctctgatc cacgctgacg ccaaacgcaa
     6241 agacatcggc aagataaccg atcttccaac gcagacaatg accgcatgga tgacactcaa
     6301 accatgggag ccgttaagcg taacgctgtc ggaagaggcg cgttcctcca gctacagcaa
     6361 cagtgacggt tcacaaaaag ccgccggttt tgcggtgacc cacattcgag ccgattacac
     6421 cttaggtcat ggcttcagcg tcaatgcgtc ggtcaataac ctgtttgata cccaatatgc
     6481 ctacagtgaa gggtttattg aagaaggtcg caatttctgg gcaggtgtcg agtatacgtt
     6541 ctgatgtgaa agcggatagc ggcatctgta tcgctatccg ccttatgaaa atcttattct
     6601 cttcgcagga ggcggaatac gcccagcacc aggatagcgc ccaccaccgc caccaggaag
     6661 ctgtgcaaat taaaaccgct gatggagccg tcaatgccaa acatggtcgc cagccagccg
     6721 ccgaccaccg caccgactat cccgagaata caggtcagga aaaatccacc accatcacgc
     6781 cccggcatga ttagcttgcc gataatgccg gctatcaggc caaaaataat ccaggcaata
     6841 attcccatgt tcaggccctc gtttcgatga atcggtcgca agaaatgctt acgtcaacgc
     6901 tctaagtata ggcaagggcg ggatcgatgc tgggcgtttg tcggctgatt taagaacaac
     6961 atgtaaaaaa tattcacgca tttgtattaa gttttgttaa ctatgaccga taaaccaaag
     7021 acagtttgtc agtcaggagt ttttccgcgt gagtcattac catgagcagt tcctgaaaca
     7081 aaatccgtta gccgtcctgg gcgtgttacg cgatttgcac aaagccgcaa ttcctttgcg
     7141 tctcagttgg aatggcgggc agctgatcag caaaatactg gcaataaccc cggataaact
     7201 ggtgctggat ttcggcagtc aagccgaaga caacatcgcc gtgctaaagg cacagcacat
     7261 taccattacc gccgaaactc agggtgcgaa agtcgagttt actgttgaac aactacagca
     7321 gagtgaatac ttgcagcttc cggcatttat taccgtaccg cctcccacct tatggtttgt
     7381 acaacgacgc cgatatttcc gcatctccgc cccactccat ccgccttatt tttgccagac
     7441 caaactggcg gataacagta cgttacgttt ccgcttgtat gatttgtcgt taggcggcat
     7501 gggcgcatta ctggaaacag caaagcctgc cgaattacaa gaaggtatgc gcttcgctca
     7561 aattgaagtc aacatggggc aatggggtgt ttttcacttt gacgcccagt taatctccac
     7621 cagcgagcgc aaagtgattg atggcaagaa tgaaaccatc accactcccc gtctgagctt
     7681 ccgttttctt aacgtcagcc cgacggtgga gcggcaatta cagcggatta ttttctctct
     7741 cgagcgagaa gcccgggaaa aagcggacaa agtgcgcgac tgatttacat cgcgtccagt
     7801 gcctgctcaa gtttgtagat atagcgcgga gcctgcggcg caggatgatt tcgcgctacg
     7861 tgatcgagga actcgtcagc atccaaatcg ttgattttgc tgatcgcctt tttccgatct
     7921 gacgagaaag tccgtagcag cgcacctgcc ccgttagcgt atgacaccac cagcgcatat
     7981 tgcagtacct tcggatcttc aatgcctgcc agcgggccgg tttccagaat attcaggtaa
     8041 gccgccccca ttgaaatatt acgctctgga tttttcagct cgctggtcgt cggctcacca
     8101 ctccagccca tacggcgata aacatcacgt ccggaggttg aagcttttaa ctgcatcaaa
     8161 ccaatggcat tcgatttact caccgcgttc ggattaccac ccgattcgat agcgataatc
     8221 gccgtgatca attgtggatc gacgccccag gctgcaccgg ctttctggct tattggcatc
     8281 cactgcatcg cacgttgcac cggaactttc gcgttccacg gcgggttcgt atagtcatgc
     8341 tttgatgaac aacccgctaa taacacaatc aaaaaggcaa accatctcaa tttcacttct
     8401 tctatcctta tagcctggaa tcgccgttga ggtgacaacg gcacacctaa gcggcatgat
     8461 atacatttcg tttctgttgt cagcaaggaa ttgccatgtc tctgtttcac ttaattgccc
     8521 catcgggtta cagcattaaa cagcacgccg cgctgcgtgg tatccagcgc cttaccgacg
     8581 cggggcatca agtcaataac gtcgaggtta ttgcccgtcg ttgtgagcgt tttgcgggta
     8641 cggaaacgga gcgtctggaa gatctcaatt cccttgccag actgactacc cccaacacca
     8701 tcgtgctggc tgtacgcggc ggttacggtg ccagtcgttt actggcagat attgactggc
     8761 aggcactggt ggctcgccaa caacatgacc cgttactcat ttgcggacat agcgatttta
     8821 ccgccattca gtgcggtctt ctggcgcaag gcaatgtcat cacctttagc ggccccatgc
     8881 tggtagcaaa ctttggtgcg gatgaactga acgcctttac cgagcaccat ttctggctgg
     8941 cgttacgcaa tgaaaccttc accattgaat ggcaagggga aggcccgaca tgtcagaccg
     9001 aaggcacgtt gtggggaggc aatcttgcga tgctgatttc actgattggt acaccgtgga
     9061 tgccaaaaat tgagaacggt attctggtgc tggaagatat taacgtgcac cctttccgcg
     9121 tcgaacgtat gctgttgcag ctctatcatg cgggaatttt gccacgtcag aaggcgatta
     9181 ttctcggtag ctttagcggc agcacgccca atgattatga cgcaggttac aacctggagt
     9241 cagtgtacgc gtttttgcgt tcccgcctgt cgattccact cattaccggc ctcgattttg
     9301 gtcatgaaca acgcacggtt acgctgccgt tgggcgcaca tgccattctg aataacaccc
     9361 gggaaggcgc tcagttgacg attagcggtc atcctgttct taaaatgtaa aaaaatcacg
     9421 cttcagggct aagtaaaacg ctgtctctgc cgctaatatg gtagggttta tataagagac
     9481 agcgtaatca ggagtaaccg accattggat gccacaacaa taattagcct tttcatctta
     9541 ggatccatcc ttgtcaccag cagtatatta cttagttcat tttcttcccg tcttggcatt
     9601 cctattctgg ttatttttct ggcgatcggc atgctggcgg gagtcgatgg cgtcggcggt
     9661 atcccgtttg ataattaccc cttcgcctac atggtaagta acctggcgct ggcgattatt
     9721 ttgctcgacg gcgggatgcg cactcaggcc agttcctttc gtgtggcgtt aggaccggcg
     9781 ctgtcgctgg cgacgctggg cgtacttatc acctctggtt taaccggcat gatggcggcg
     9841 tggctgttta atcttgattt gattgaaggc ttattaatcg gcgctatcgt cggctccacc
     9901 gatgctgcag cggtcttttc tctgctgggt ggtaaggggc ttaacgaacg tgttggctcg
     9961 acgctggaaa ttgaatccgg cagtaatgat ccaatggcgg tctttctgac gattaccctg
    10021 attgcaatga tccagcagca tgaaagcagt gtcagttgga tgttcgtggt cgatattctg
    10081 cagcaatttg gcctcgggat tgtcatcggt cttggcggtg gttatttact gctgcaaatg
    10141 attaatcgca tcgccctgcc tgccggatta tatccattgc tggcattaag cggcggtatt
    10201 ttaatttttg ctttaaccac ggcgctggaa ggcagcggta ttctggctgt ttatctgtgc
    10261 ggttttctgc tgggtaatcg gccgattcgc aaccgctacg gcatcctgca aaatttcgac
    10321 ggcctcgcct ggctggcgca aatcgccatg ttcctggtgc tggggctatt ggttaaccca
    10381 agcgatctgc tgcctattgc cattccggcg ctcattttgt cggcatggat gatattcttc
    10441 gcccgtcccc tttcggtatt tgctggattg ctccctttcc gcggtttcaa tctgcgtgag
    10501 cgcgtgttta tcagctgggt gggattacgc ggcgcggtgc caatcatcct agccgtgttc
    10561 ccgatgatgg cggggctgga gaatgcgcga ctgttcttta atgtcgcctt ctttgtggtt
    10621 ctggtttcac tgctcttgca gggaacatca ctctcgtggg cggcgaaaaa agccaaagtg
    10681 gtggttccgc ccgtcggacg tccggtgtca cgcgttggcc tggatattca tccggaaaat
    10741 ccgtgggagc agtttgttta tcaattgagt gctgataaat ggtgtgtagg tgcggcactg
    10801 cgcgatttgc atatgccaaa agagacgcgt attgcagccc tgtttcgtga taaccagttg
    10861 cttcatccta ccggcagcac ccgactgcgc gaaggcgatg tattgtgtgt aattggtcgg
    10921 gaacgcgatc tcccggcgct cggtaaactg ttcagccagt cgccaccggt cgcgctggat
    10981 caacgcttct ttggtgactt cattctcgaa gccagcgcca aatatgctga tgttgcgctg
    11041 atatatggcc tggaagacgg gcgagaatat cgtgataaac agcaaacgct gggtgaaatt
    11101 gtccagcagt tgctaggcgc ggcaccggtt gtcggtgacc aggtagagtt tgccgggatg
    11161 atctggacgg tggccgagaa agaagataat gaagtgttga agattggcgt tcgggtggct
    11221 gaagaggaag ctgaatctta aatttaatgc agcatctatc gccggatgcg atgctttact
    11281 gaacgcagcc tgagcctgta ataaacttgt catttcctga ttttgatatg ctcatcccaa
    11341 catcagaaga aggttaaatt atggatgaca aaccattgca ggctctggct aatgaactca
    11401 aaacaccggc tcaagaaagt cagcgtaagg gctttttccg actgtaacag gtaataaaaa
    11461 ccagccccgc attaccggtc aaccatcagg ggcccctgcc tgacatcgcc tgaacatatt
    11521 cccccacgca acctgccgca ctgacatagc ccacacaata ggcgtttcat ccgcctgtag
    11581 caccggatgc atgagcgatt tcttatagtt attcctttgc ttaataacga ttatttccgg
    11641 gtcgttcact ctcgtatttt ctttaatacg tcagagctac ctaggccgta atacagcatg
    11701 taccagaccc gacacggcat ttctgaccag gaaaaaagtc aacgatgagt agtgatatca
    11761 gcgcaaatga cgtcctgtgg ctcctttgcg gtgaccacat tcacgtctcc ggcgctgcca
    11821 gaggatattt atttcaagga aacgtagcat tatggctagc tttaaagaca tctgcccatt
    11881 cgcccccgcc attcccccat cttccgttaa cccaggcgta cgacgcccgc cagggcggct
    11941 cttctcaaga atcaaatttt gcagaacatg aagtatctca gcccgcaaaa attcagctgg
    12001 ggtgatgccc cctggcagat cattgacctc agtatcgcgg gcaaagtgaa catacaggtg
    12061 gacaacaata ccatcatcac gttggggact cgtctgaatc agcaacataa cgagttcatg
    12121 atggtcgcca aatggtgcga atgggctata cagcaggatg gtttgcaaga aaacctgcaa
    12181 aaaaacctgt acgaaatcct ggaagaaaac cagcaaaaca aacagtcaga aattccccaa
    12241 gaagacctga aagaaagtct ggaagaaatc aaggaaaata tcctggaaga aaacctgcca
    12301 gcaagccgga ttgaaaaccg ggcagaagca ttgcgcagaa tgaaggaatg tctcattaca
    12361 agacggagta tgctcaatct tagcaacctg ggattgactt cactccctga gaatttgcct
    12421 ccacatctga ttgaatttta ctgcagtaaa aacgtgttga ccgcgttacc gaaggtgatg
    12481 ccaaagtggc tgctggtgct tgactgtaca gataacgtct tgatcttgtt accgaaggtg
    12541 cagccctcaa aactgatggt actgaagtgc tatgaaaaca gtattatctg gctgcctgag
    12601 ctgtcgacta acctgagagt gattaactgt tctgaaaact tcttgcaatt tttaccgccg
    12661 tcgatgcccc agtacctgta taaactatcc tgtgccggca acaatattaa ctcaatacct
    12721 gatgagatgc tggagaactt gactcgcctg aaagtatttg actgttctag taacgatttg
    12781 atctcttcac cacggctgcc gcccaaacta ataatttact actgtggaga aaacaagttt
    12841 aaaacggtac aggtgccgca gccccagagc ctgaaggtgt ttgactgtaa tggtaacccg
    12901 tgggacaaag acaatttacc gacgctgctc aaagccgtcg agggcctgaa aaaacagcag
    12961 ggcctgaagg accttttgga ctttttgcac aaggaaggct aggttgacct ggaaggactc
    13021 gaggagctgg aggaccttat ggatctggag ttcctggatg acccggaact cctggagcgc
    13081 gtgaaggtac aggaggacct ggagctcctg gatcaacagt tgggcctgtt gagtctggaa
    13141 agacaacagg actcgcagcc tgttaatcaa caatctgaac atgaacccga atctgcatca
    13201 agggtgaagc gtgatttatc tgaagtcgac tccgagtcaa caatgaagcg taagcgtttt
    13261 atgtagaacg cctgtgcggc gagttcaggt tccggggaaa tagtgtccat tcttttaatg
    13321 gacactatca tgggcaatgg ctctgtcaca ttaacaacta cagttgctga ccagccggat
    13381 ggatgatttt ttcgcaacca gaagacgctg cctccgatcc ggcttacaac aagttacact
    13441 gtcacaaccg ggacgcgtaa cgccagtgcg cacatcaact catagcccac cgttccggca
    13501 gcggcggcga catcatcaat tttgatctcc ttgccccaca gctcaaccgg cgtaccaatc
    13561 cccgcctgcg ggcaaggcgt taaatcaacc gccagcatat ccatcgagac ggtccccacc
    13621 gtcatggtgc gcacgccgtc cactaaaaca ggagcaccgg taggcgcatg gcgcggataa
    13681 ccgtcggcgt atcctgcggc gacaatgccg attcgctgtt catcgcgcgc agtatagcga
    13741 ccgccgtagc ccacacgctc gcccgctttt agcgtctgga caccaataat ctcactgctt
    13801 agcgtcatca ccggacgtaa tccggtattg gcgatatcac gccactgacc ggacggcgaa
    13861 gcgccataca aaataatgcc aggccgaacc cagtcaaaat gcgcttccgg gtgccacaga
    13921 gtcgccgccg aattggacaa cgaacgccga cactccagcc cctccgccgc ctgctcaata
    13981 cgcgccatcg cgccggaaat tccatcagga tgttccgctt cagcaaaatg cgacatcagg
    14041 gtcatttcgc caacattcgc cattgcccgc aactgctgcc agacggtaag cacgcgatcg
    14101 gactggaagc ccaaccgatt catcccactg ttcactttaa gataaatatc caacggtgct
    14161 tttagccgcg cattttgcag tgctttgagc tgccagttgc tgtgtacgca ggtggtcagg
    14221 cggtgctggt cataaatctc cagatcctga gcatggaaaa atccttccag catcaggatc
    14281 ggccccttcc agccgcgctc acgtaacgtt attgcctctt ccaggttaag taatgcaaag
    14341 ccatcggtgg ccccgagcgc gctccagata cgctcaatac catgcccgta agcgttcgct
    14401 tttaccaccg accagacgcg cgcgtgcggc gcggcctggc ggacaatgga cagattctgt
    14461 tttaatgcct gcagatcgag gctggcctgt atcggacggg tcatctcgtt tccttagctg
    14521 tgtgcgccat gtaaatggcc cggacgtgat ggcgtaaatc cacggctgta gcgcgctacg
    14581 cttagatcct catatgggat cgctggcgtg cgaccagaga gcagatcgct taacaactga
    14641 ccggaaccgc aagccatcgt ccagccgagc gtgccgtgac cggtattcag ccacagattt
    14701 ttaaagcgtg tactcccgac aaccggcgta ccgtctggcg tcatcgggcg caaacccgtc
    14761 cagaaagtcg cctgctcgac atgaccgccg cgtggataga gatcgcgaac caccatttcc
    14821 agcgtttcac gacgcggttg caacagctcg gtgttaaaac caacaatttc cgccattccg
    14881 ccaacacgaa tgcggttatc gaaacgggta atggcgattt tgtaggtttc atcaagaatg
    14941 gtggatactg gcgcaccatc ttcctgcgca attggaatag tcagcgagta gcctttcagc
    15001 ggatacaccg gaatatcaac aatgcctttg agcatcgccg tcgagtaaga accaaacgcc
    15061 atcacatacg catcggcctt aatcacttca tcgccaaact tcacgccgta gatttgctcg
    15121 ccgtcgcaaa gcagttggtc aacgggcgta ttaaagcgga atttaacccc cgcctgctct
    15181 gccatccgcg ccagattctg ggtaaacagc tggcagtctc cggtttcatc attgggtaac
    15241 tgcaggccgc ccgtcagttt gtgcgctact tctgccagcg cgggatccac ttccgccagg
    15301 cggctggatt ccagcagctg atacggtacg ccggcatctt ccagcacggc gatatcgcgg
    15361 gtcgcatttt catactgttg ttcggtacgg aacagttgca gcgtcccacc ctgacgccct
    15421 tcatactgaa tattggtttc ggcgcgcaat gctttcaggc aatcacggct gtattccgcc
    15481 agacgcacca tccgcccttt gttttccatg tagtggctgg tgtcgcagtt acgtaacatt
    15541 tgccacatcc atttcaactg aaactgcgta ccgtcgagac gaaccgccag cggcgcatgg
    15601 cgctggaaca tccatttaat cgcttttaaa ggcacacctg gtgctgccca cggtgcagca
    15661 tatccggggg agatttgccc ggcattcgcg gcactggttt ccagggcggc ccccggctcc
    15721 cgatcaatga cggtgacctc atgtcctgcc tgatttaagt accaggcgct ggcaacgcct
    15781 accacaccac ttcccagtat gacaactcgc atagccactc cgataacggt agatacagta
    15841 aaagaataat aatctaatta catcttgata actcagttga aaatattatt caacatacgg
    15901 cttttttatg gtgagctgac tcacatctcc ctgactctgc ggcaatatgg cgtctttagt
    15961 atctcctgct gaacgccctt tttatccagc ataaaattcc gttcagaagc ggattagtgg
    16021 cactctgata ataagaaatc gcaaagaaaa aaattctccg gcgacgcggt ttttaacagg
    16081 tgttctatgc ttgaaatgag gtgctccaca cagagagcgc cgacaacaat gaggtgcgcg
    16141 tatggcgacg atcgattcta tgaataagga caccacacgt ttgagcgatg gacccgactg
    16201 gacgttcgac ctgctggatg tttatctggc agagatagac cgggtggcga aactctaccg
    16261 gctggatacc tacccgcacc agattgaagt gataacctca gaacagatga tggatgccta
    16321 ttccagcgtc ggcatgccaa ttaactatcc gcactggtca ttcggtaaaa agtttatcga
    16381 gactgaacgg ctgtataagc acggtcagca agggctggcc tatgaaatcg tcattaactc
    16441 taacccgtgt atcgcttact tgatggaaga gaacaccatt actatgcagg cgctggtgat
    16501 ggcacacgcc tgctatggac ataactcttt ctttaaaaac aattgcttat tccgcagttg
    16561 gaccgacgcc agttcgattg tcgattatct gatttttgcc cgtaaatata ttaccgagtg
    16621 cgaagaacgt tatggcgttg atgaagtaga acggcttctg gactcgtgcc acgcgctgat
    16681 gaactacggc gtggaccggt acaaacgccc gcaaaaaatc tcgctgcaag aagagaaagc
    16741 ccggcagaaa agtcgcgaag agtatctaca aagtcaggtc aatatgctct ggcgtaccct
    16801 gccgaagcgc gaggaagaga aaacggttgc tgaagcgcgc cgctatccgt ccgaaccaca
    16861 agaaaacctg ctctatttta tggagaaaaa tgcgccactg ctggaatcat ggcagcgtga
    16921 aatcctgcgt attgtgcgta aggtgagcca gtatttttat ccgcaaaaac agactcaggt
    16981 gatgaacgaa ggctgtgcga ccttctggca ctataccatc cttaaccatc tgtatgatga
    17041 agggaaagta acggaacgtt ttatgctgga gtttttgcac agccacacca atgtggtctt
    17101 ccagcccccc tataacagcc cgtggtacag cggcatcaac ccgtatgccc tcgggttcgc
    17161 catgttccag gatattaaac ggatttgtca gtcgccaacg gaagaagaca aatactggtt
    17221 cccggatatc gccggttccg actggctgga aacgctgcat ttcgcgatgc gtgatttcaa
    17281 agatgagagt tttatcagcc agtttctgtc accgaaagtg atgcgtgatt tccgcttctt
    17341 caccgtgctg gatgatgatc ggcataatta tctggagatt tccgctattc ataatgaaga
    17401 aggttatcgg gagatccgta accggttatc gtcgcaatat aacttaagta atctggagcc
    17461 gaatattcag atctggaacg tggatttgcg cggcgaccgt tcgctgacgc tgcgttatat
    17521 tccacataat cgcgcaccgc tggatcgggg gcgcaaagaa gtcctgaagc atgtgcatcg
    17581 cctgtgggga tttgatgtga tgctcgaaca gcaaaacgaa gacggcagca tcgagttgct
    17641 ggaacgttgc ccgccaagaa tgggaaatct gtaaaaacaa caaaaaaccc ctcgtttgag
    17701 ggggttgctc tttaaacgga agggattatc gcccctgaat ggctaaatca cccggcagat
    17761 ttttctgcat ccggtgccaa atctcgccac tctcatgccc atagcgacgc actgtttcgt
    17821 acacctgatc gtgcgcgcct tcactgcaca acgccgacag tttgtggtag aagcccagcg
    17881 ccagactgcg cgcttccgga ttggcgaaat agtgacgacc aatgcgcgta tacagccctt
    17941 tcatcccgtt aaggatcaga ccgtaaatcg ggttgccgga agcaaacgcc aggccgcgga
    18001 atatgttgta atccagctcg gcaaaggcat cggcgtgatc ggccacttca ttagcggtag
    18061 ccagcacttc ctgcgcttta tcgggatgct gacgaaacgc ggtgcgaata aaaatagtgg
    18121 aaatattggt acgcaccgac agcaaattat caataagctg cggcacactt tcgtgatcca
    18181 gtcgcgccag tgtttcaagg atatttaaac cggaagtttc ccagaaatta ttcaccttcg
    18241 tcggcttgcc atgttgaatg gtcaaccagc catctcgtgc cagacgctgt aacacttcac
    18301 gtaacgtagt acgcgttacg ccaattaatt ctgaaagttc acgttctgcg ggcaaaatag
    18361 tcccgggagg gaagcggtta ttccagatac tttcaataat gtactcttcc gcgaaacccg
    18421 ccgggctttg cgccttaatg accatagtga gatttccata acacagcaaa acaaagttgg
    18481 actcatcata ccagagggct aactacgctg atagcagaca aaaagaagaa aaagggatca
    18541 aggcttcatt ttccgcatct ctttcccctt gactatcaag acttgcctca ctcctgcctg
    18601 accgttaagc tttgcgttta aacataaaaa catcattgat tatttgtaag gtaagggaaa
    18661 ccatcatgga gatctcctgg ggccgcgcgc tatggcgcaa ttttttgggc cagtcccccg
    18721 actggtacaa actcgccctc atcattttct taatcgtaaa cccgttaatt ttcctcatca
    18781 gccctttcgt cgcgggctgg ttgctggtag cggaatttat tttcactctg gcgatggccc
    18841 tgaaatgcta cccgctactc cccggtggtc tgttggctat cgaagcggta ttcatcggca
    18901 tgaccagcgc ggaacacgtc cgtgaagagg tggcggcaaa tcttgaagtc ttgctgttat
    18961 tgatgtttat ggtggcgggt atctatttta tgaaacagct gttgctgttc atatttaccc
    19021 gtttgctgtt aagcattcgc tccaaaatgc tgctgtcgct ctctttttgc gtggcggcag
    19081 cgttcctctc cgcgttcctc gatgccttaa ccgtcgtggc ggtggtgatc agcgttgcag
    19141 tcggttttta tggtatttat catcgcgtag cctcttcccg taccgaagac accgacctgc
    19201 aagacgatag tcatatcgac aagcattaca acgtggttct ggaacagttc cgtggctttc
    19261 tgcgtagcct gatgatgcat gccggtgtcg gcaccgcatt aggcggcgta atgaccatgg
    19321 tgggcgaacc acagaacctg atcatcgcta aagcggctgg ctggcatttt ggcgatttct
    19381 tcctgcgcat gtcgccggtg accgttccgg ttctgatttg tggcctgtta acctgcctgc
    19441 tggtagagaa gctgcgttgg tttggctacg gtgaaacgct gccggagaaa gtccgcgaag
    19501 tgttgcaaca gtttgacgat caaagccgcc accagcgtac ccgtcaggat aaaatccgtc
    19561 tgattgtcca ggcgattatt ggcgtctggc tggtgactgc gctggcgttg catctggcgg
    19621 aagttggctt gattggtttg tcagtcatta ttctggcaac atcattgacc ggtgtcaccg
    19681 atgaacatgc tatcggtaaa gccttcaccg aatctctgcc attcaccgca ctgttgacgg
    19741 tgtttttctc ggtagtcgcg gtgattatcg accaacaact gttttcgccg attattcagt
    19801 ttgtgttgca ggcatctgaa catgctcagc tgtcgctgtt ctatattttc aacggtctgc
    19861 tgtcatccat ttcggataac gtcttcgtgg ggacgattta tatcaacgaa gcgaaagcgg
    19921 caatggaaag tggcgctatc acgttgaagc aatacgagct gctggcggtc gccattaata
    19981 ccggtaccaa tctgccctct gtcgctacgc cgaacggtca ggctgcgttc ctgttcctgc
    20041 tgacctctgc actcgcgcca ttgattcgcc tctcttatgg ccgcatggtg tggatggccc
    20101 tgccttacac cctcgtactg acactcgtcg gcttgctctg cgtcgagttt acgcttgccc
    20161 ctgtaaccga atggtttatg caaatgggct ggatagcaac gctttgataa caacttaccg
    20221 ggcatataaa tgcccggttt gccttttcgc ccgataattg tccaattgcg tcctattttt
    20281 gctgcctcct ggtggcgcag cgaatgaatt ggtttaaact gcgcactcta tgcatattgc
    20341 agggaaatga ttatgttgcg atttttgaac caatgttcac aaggccgggg cgcgtggctg
    20401 ttgatggcgt ttactgctct ggcactggaa ctgacggcgc tgtggttcca gcatgtgatg
    20461 ttactgaaac cttgcgtgct ctgtatttat gaacgctgcg cgttattcgg cgttctgggt
    20521 gctgcgctga ttggcgcgat cgccccgaaa actccgctgc gttatgtagc gatggttatc
    20581 tggttgtata gtgcgttccg cggtgtgcag ttaacttacg agcacaccat gcttcagctc
    20641 tatccttcgc catttgccac ctgtgatttt atggttcgtt tcccggaatg gctgccgctg
    20701 gataaatggg tgccgcaagt gtttgtcgcc tctggcgatt gcgccgagcg tcagtgggat
    20761 tttttaggta tggaaatgcc gcagtggctg ctcggtattt ttatcgctta cctgattgtc
    20821 gcggtgctgg tggtgatttc ccagccgttt aaagcgaaaa aacgtgatct gttcggtcgc
    20881 taatccattc ggcgctcctg cgggagcgct tttttcctgc cgctatattt atttgacccg
    20941 cagtaaatca gaacttcgcg ttgtataacg tggtgaaagc atagctcgct tcatctgcca
    21001 ttgttgttgg atcccctgcc cggcaaaata gagtgttcct ctgccctctt tagcattcag
    21061 agtatccatt accgccatca attgctcact cccggggcgc ggtgcgttgt catcgaataa
    21121 attgagctgc gcgactccct gactgaagaa atcccccagc atcacgcccg ctttttggta
    21181 gcgatggccc gcttgccaga tggcatccag agatcgcgta gcagcgttaa tgatatccct
    21241 gctgtcctga gtgggcgtca gcagttttac cgacgcgcta ttgccgtaat aaggttcatt
    21301 gagcgcaaat ggtgacgtct taataaatgt ggagataaac cgacaatatt gatgctcact
    21361 gcgaagtttt tccgccgccc gggcagcgta actacaaatg gcctgccgca tcgacgtata
    21421 atccgtgatg cgttcaccaa acgagcggga acagataatt tcctgcttcg tcggtgcaaa
    21481 ctcttccagt tgcaaacagg gttcgccgcg cagttcacgc accgttcttt cgagcatgac
    21541 attaaaatgt ttacggataa accggatatc tgtatccgcc aaatcgagaa cggttttgat
    21601 ccccatcgcg tccagttttt tgctgatccg ccgtccaatc ccccagacgt catccacggg
    21661 gagagcagac attaatttac gctggcgttc cagatttgat aaatccacca ccccacccgt
    21721 ctgccgctgc catttttttg ccgcatgatt ggcaagctta gccaacgttt tggtctgggc
    21781 gatccccaca ccaacagtaa gatgggtacg ttgtagcacc gttgcgcgaa tttctctgcc
    21841 aaaatcagtc agatcgcgac aattacgcac acctgtcaga tcgcagaatg cttcatcaat
    21901 actgtaaatc tcgacgcggg gcgatagttc ttccagcgtc gacatcaccc gattgctcat
    21961 gtctgcgtaa agctcataat tgctgctaaa gcaaaccaca ccacagcgac gaaacagatc
    22021 tttttgtttg aaccagggat cgcccatttt aacgcccagc gcctttgcct cagcgtttcg
    22081 ggcgataacg caaccgtcat tattcgatag cacaaccacc ggtttacccc ataaatcagg
    22141 gcgaaacacc gtctcacagc tggcataaaa cgcgtttaca tcacagaggg caaacatcag
    22201 cgcatcgcct taacgacgtg gatcaccaca ccaaagacat ccagcgtatc ttcgctactg
    22261 atggtaatgg gcgagtacgc actgttcatg ggaataagct gtaccgtcgg gcgtagttgc
    22321 aattttttca ccgtaaactc gccgtcaaca gcagcgatga caatatcacc atggctggcg
    22381 gtaatagcac tatcgacaat cagtaaatca ccgtcactaa ttccaccatc aatcatagaa
    22441 tcaccacttg ctttgacgaa gtaagtcgcg ctgggatgct ggatcaacag ttgattcaga
    22501 tcgatgcgct gttcaacgta atctgctgcc ggtgaaggaa agccacactg aacaagatcg
    22561 ctaaatagcg gaaaagtcac aatttcgcgg agatccgcag gcttgataaa caacataata
    22621 atctgcctga agttatactg tttttatata cagtggtctg ttcttgccag cagatcaata
    22681 ctgattcagg ctatcaatat ttgtcgctgc atagactgct gatttttcgt tctcttatcc
    22741 tgtgctcacg tggccttctg gcgacgacgc tcatccagca gaaatgaaaa atatcacccg
    22801 gcaaaaaaat agaatagaag cattcgccat aatgacatta aacattgttt gatatttatc
    22861 atattaatag aaataaagac attga
//
LOCUS       DAZPEU010000052        22410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPEU010000052 DAZPEU010000000
VERSION     DAZPEU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.052"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            82..2682
                     /locus_tag="ACHU2L_001802"
     CDS             82..2682
                     /locus_tag="ACHU2L_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR1356682.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACHU2L_001803"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACHU2L_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACHU2L_001804"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACHU2L_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACHU2L_001805"
     CDS             4302..4817
                     /locus_tag="ACHU2L_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1356683.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACHU2L_001806"
     CDS             4783..5520
                     /locus_tag="ACHU2L_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1356684.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHU2L_001807"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHU2L_001807"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR1356685.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACHU2L_001808"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACHU2L_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR1356686.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHU2L_001809"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHU2L_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR1356687.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHU2L_001810"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHU2L_001810"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR1356688.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACHU2L_001811"
     CDS             10827..12734
                     /locus_tag="ACHU2L_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1356689.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHU2L_001812"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHU2L_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR1356690.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHU2L_001813"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHU2L_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR1356691.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHU2L_001814"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHU2L_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR1356692.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACHU2L_001815"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACHU2L_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR1356693.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHU2L_001816"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHU2L_001816"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR1356694.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACHU2L_001817"
     CDS             complement(17402..19162)
                     /locus_tag="ACHU2L_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1356695.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACHU2L_001818"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACHU2L_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR1356696.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHU2L_001819"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHU2L_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR1356697.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHU2L_001820"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHU2L_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR1356698.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22410
                     /gene="sxy"
                     /locus_tag="ACHU2L_001821"
     CDS             22005..>22410
                     /gene="sxy"
                     /locus_tag="ACHU2L_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR1356699.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGE"
BASE COUNT         5722 a         5258 c         5871 g         5559 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag acatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg
//
LOCUS       DAZPEU010000053        22014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPEU010000053 DAZPEU010000000
VERSION     DAZPEU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.053"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(498..1589)
                     /gene="ychF"
                     /locus_tag="ACHU2L_001822"
     CDS             complement(498..1589)
                     /gene="ychF"
                     /locus_tag="ACHU2L_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR1356700.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1706..2290)
                     /gene="pth"
                     /locus_tag="ACHU2L_001823"
     CDS             complement(1706..2290)
                     /gene="pth"
                     /locus_tag="ACHU2L_001823"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR1356701.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2568..2846
                     /gene="ychH"
                     /locus_tag="ACHU2L_001824"
     CDS             2568..2846
                     /gene="ychH"
                     /locus_tag="ACHU2L_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR1356702.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2901..4580)
                     /gene="dauA"
                     /locus_tag="ACHU2L_001825"
     CDS             complement(2901..4580)
                     /gene="dauA"
                     /locus_tag="ACHU2L_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR1356703.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4705..5652)
                     /gene="prs"
                     /locus_tag="ACHU2L_001826"
     CDS             complement(4705..5652)
                     /gene="prs"
                     /locus_tag="ACHU2L_001826"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR1356704.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5803..6654)
                     /gene="ispE"
                     /locus_tag="ACHU2L_001827"
     CDS             complement(5803..6654)
                     /gene="ispE"
                     /locus_tag="ACHU2L_001827"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR1356705.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6654..7277)
                     /gene="lolB"
                     /locus_tag="ACHU2L_001828"
     CDS             complement(6654..7277)
                     /gene="lolB"
                     /locus_tag="ACHU2L_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR1356706.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7491..8747
                     /gene="hemA"
                     /locus_tag="ACHU2L_001829"
     CDS             7491..8747
                     /gene="hemA"
                     /locus_tag="ACHU2L_001829"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR1356707.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8789..9871
                     /gene="prfA"
                     /locus_tag="ACHU2L_001830"
     CDS             8789..9871
                     /gene="prfA"
                     /locus_tag="ACHU2L_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR1356708.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9871..10704
                     /gene="prmC"
                     /locus_tag="ACHU2L_001831"
     CDS             9871..10704
                     /gene="prmC"
                     /locus_tag="ACHU2L_001831"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1356709.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10701..11093
                     /gene="sirB2"
                     /locus_tag="ACHU2L_001832"
     CDS             10701..11093
                     /gene="sirB2"
                     /locus_tag="ACHU2L_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR1356710.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11097..11906
                     /gene="sirB1"
                     /locus_tag="ACHU2L_001833"
     CDS             11097..11906
                     /gene="sirB1"
                     /locus_tag="ACHU2L_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR1356711.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11942..12796
                     /gene="kdsA"
                     /locus_tag="ACHU2L_001834"
     CDS             11942..12796
                     /gene="kdsA"
                     /locus_tag="ACHU2L_001834"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR1356712.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12945..13052)
                     /locus_tag="ACHU2L_001835"
     CDS             complement(12945..13052)
                     /locus_tag="ACHU2L_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1356713.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(13457..14557)
                     /gene="chaA"
                     /locus_tag="ACHU2L_001836"
     CDS             complement(13457..14557)
                     /gene="chaA"
                     /locus_tag="ACHU2L_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR1356714.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14827..15057
                     /gene="chaB"
                     /locus_tag="ACHU2L_001837"
     CDS             14827..15057
                     /gene="chaB"
                     /locus_tag="ACHU2L_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR1356715.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15215..15910
                     /gene="chaC"
                     /locus_tag="ACHU2L_001838"
     CDS             15215..15910
                     /gene="chaC"
                     /locus_tag="ACHU2L_001838"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR1356716.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(15954..16307)
                     /gene="ychN"
                     /locus_tag="ACHU2L_001839"
     CDS             complement(15954..16307)
                     /gene="ychN"
                     /locus_tag="ACHU2L_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR1356717.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16492..17886
                     /gene="ychO"
                     /locus_tag="ACHU2L_001840"
                     /gene_synonym="iatA"
     CDS             16492..17886
                     /gene="ychO"
                     /locus_tag="ACHU2L_001840"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR1356718.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17887..18537)
                     /gene="narL"
                     /locus_tag="ACHU2L_001841"
     CDS             complement(17887..18537)
                     /gene="narL"
                     /locus_tag="ACHU2L_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR1356719.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18530..20326)
                     /gene="narX"
                     /locus_tag="ACHU2L_001842"
     CDS             complement(18530..20326)
                     /gene="narX"
                     /locus_tag="ACHU2L_001842"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR1356720.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            20665..>22014
                     /gene="narK"
                     /locus_tag="ACHU2L_001843"
     CDS             20665..>22014
                     /gene="narK"
                     /locus_tag="ACHU2L_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR1356721.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     "
BASE COUNT         5447 a         5478 c         5688 g         5401 t
ORIGIN      
        1 gtatctgcat aacaaaaaca atatgcgcca tttttgcatt actaataaga aaaagcaaaa
       61 aacatcgata ttcatttttg ttcaatggat tttctggcct atcgttatta ttgtgctact
      121 ggttatcatt ttcatcttgc caggatcgca cgtaatcacg ttattcgggg actttatctg
      181 cgggatatta cccttcaggt aagtcctttt ctcgccagca tattggagcg agatcaatat
      241 tgatcagctg tgaagagggc tgatgtgtag tggaacaaaa actgacgtta tatcactgct
      301 aatgagatat cagtcatctg aacacttatg tctttaatta ttctcgtggt tcactgtagg
      361 caataagcac aaaagtgtag gatgttacaa gaatgattag gactcggtga aatgaaaaat
      421 ccacgcaatt gcgtggattt tatatacttt tgcgctcttc atgagattta gcgaaacctc
      481 atgagacaat aaattaatta gacgttgaaa aggaagttca tcacatcgcc atctttcacg
      541 atgtaatctt taccttctgc acgcattttg cctgcttctt tcgcgccttg ttcacctttg
      601 taagtgatga aatcttcaaa cgagatggtt tgtgcacgga taaagccttt ttcaaaatca
      661 gtatggattt tgcccgctgc ctgcggcgcg gttgctccaa ccggaatggt ccatgcacgc
      721 acttctttca ccccagcggt gaagtaagtt tgcaggttca gcagcttata accggcacgg
      781 atcacacggt tcaggcccgg ttcttccagc ccaagctcct gcataaactc gtcacgttct
      841 tcgtcgtcca gttcggcaat gtctgcttca acagcagcac aaaccggaac cacaacagaa
      901 ccttctttcg ccgcgatttc acgcacctgg tcaagatatg ggttgttttc aaaaccatct
      961 tcgttgacgt tggctatgta cattgttggt ttcagcgtca ggaagctcag gtaacgaata
     1021 gccgctttct cttcagcgct taaatccagc gcgcgcagca tacctgcgtt ttccagctgg
     1081 ggcaggcatt tttccaggac cgccagctca gctttcgcgt ctttatcgcc acctttggct
     1141 ttcttctgta cgcgatgaat cgcacgttcg caggtgtcga ggtctgccag cgccagttcg
     1201 gtgttgataa cttcaatatc gtcagccggg ttaactttgc cggaaacgtg aatgatgttg
     1261 tcattttcaa agcagcgaac aacgtgaccg atcgcttcgg tttcacggat gttggtcagg
     1321 aactggttac ccagaccttc gcctttcgat gcgcctttta ccagaccggc gatatcgaca
     1381 aattccatgg tcgtgggaag cgtacgttgc ggttttacga tttcagccag ttgatccagg
     1441 cgagggtcag gcattggtac gacgcctgtg ttcggctcaa tggtgcagaa tggaaagttg
     1501 gccgcttcaa taccggcttt ggtcagcgcg ttgaacaggg tagatttccc gacgttgggc
     1561 aaaccgacga taccgcattt gaatcccatg atttaaatca ccttaatatc ttaataatca
     1621 acctgttatt gctaacagat tgcagaaatg gaaataactt tgcctattat acacggcact
     1681 cggcaaaaat gccgcagaca acgacttatt gcgctttaaa ggcgtgcaat cggttcgttg
     1741 ctttggtcaa gccatctgta aaccacattt cagtacaacg cgccgcttcg tcgatggctt
     1801 catcaattaa cttctgttca ctaacaggcg gtttgcctaa cacaaaaccg acaactttat
     1861 ttttatcgcc cggatgaccg attccgatgc gtaaacggtg aaagttaggg ttattaccca
     1921 atttactgat gatgtctttc agtccattgt gaccaccatg gccaccgccc aacttaaatt
     1981 tggcgacgcc aggaggcaga tccagttcgt cgtgggccac cagaatttcg tccggattaa
     2041 tgcggaaaaa actggccatc gccgcaacgg ctttgccgct gagattcata aatgtagtcg
     2101 ggactaacag gcggacatct tcgcttccaa gggtgactcg cgaagtataa ccaaagaatt
     2161 tagcctcttc gcgcagcgga gcgcgcaaac gctctgccag taagtcaacg aaccaggcac
     2221 cagcattatg tcgcgttgcg gcgtattcag caccggggtt cgccaggccg acaatcaatt
     2281 taatcgtcac gtttttttgt cctgagtgtg tacataactg gcgcgtagtt tactggttgc
     2341 ggccccgctt gacaaaaaac tgcgtatcaa atgcagataa cgtaataatt gcctgagtgg
     2401 actattagaa agtcaaggtg ttcaggcgtt tatttgtaaa gttttgttga aataagggtt
     2461 gtaattgtga ttacgcccgc acataaccca cagggtgttg tctatacttt acacataagg
     2521 aagaggggta ttccctgtta caacccagaa agttccggag gtgacatatg aaacgcaaaa
     2581 acgcttcgat actcggtaac gtgctcatgg ggttgggtct ggtggtaatg gtggtcggcg
     2641 tggggtattc aatcctcaac cagttaccac agtttaatat gccccagtat ttcgcacatg
     2701 gtgcagtgct aagtattttc gtcggtgcca ttctctggct ggcgggtgcc cgtgttggcg
     2761 ggcatgaaca ggtgtgcgac cgttactggt gggttcgtca ctatgacaaa cgttgccgcc
     2821 gtagcgataa tcgccgtcat agctaacaaa taatgcagtt tgctgaccag tcagtttgcg
     2881 ctgactggtc aatctcgtac ttataaatcc gccatcgccg cgcgacgatt cgggaagaac
     2941 gcgaggcgtc ccgggatcgg ttgaatgcct gcgcgagcca tagttcgcag tggctggaat
     3001 tccacgttgc acacgcgcag ttcacatcct tcgggcagac gcttcacaaa acgctggaac
     3061 gcatcaagac caccagcatc aagtaccgga acggcatccc acttcagaat cacaatccgt
     3121 ttgccttcaa gacgtgactc caggtccgtg aataagcctt cagcagcagc aaaaaacagc
     3181 gggccaataa cgcgcagaac caggacatcg tctggaacat ctacgactac cggtgccagg
     3241 cgagtcatac gtgcgatacg acgcataaac agcagcgatg ccagcacgat ccccacgctg
     3301 atggcaataa ccatatcaaa cagcacggtc agtgacatac acagcagcat gacgatgatg
     3361 tcatctttcg gcgcatgacg cagcaagtcg accactttgt gcgcttcact catgttccac
     3421 gccaccatca acagcagggc tgccatggcg gaaagcggca gccaggagag cagtggtgcc
     3481 agtaccagca gggcgagaat aaccagaata gagtggatca ccgccgagat aggggaagtt
     3541 gccccggcac ggacgttagc ggcagaacgc gcgatggcag cagtagcggt aataccacca
     3601 aagaatggag cgatgatatt ccccagcccc tgaccgacca gttcactatt cgccttgtgc
     3661 ttagtcccgg tcataccatc cagtaccaca gcgcagagca gagattcgat cgccccgagc
     3721 attgccattg agaatgccgc aggcagcagg gtgcgaatag aatcccaggt tagcgtgaat
     3781 tctgaattag gcagatccca cggcagcacc agttgcggca gcagttgcgg aataccgtta
     3841 ccctgagaac catcggccag gacgtagtgg aattgcgaac cgatggtagc aacatgtccg
     3901 ccgagcaggt taacaatccc catcaccgcg caaccagcca gcaaggccgg aaggtgaccg
     3961 ggtaaacgaa tgcccagacg cggccagaaa accagaatgc caagcgtcac aatgccaatg
     4021 gcagcatcac ccacattaat ggtcggcagc gccataaata atgcgccgac tttttgtaga
     4081 taatgttccg ggacatgggc catttgcaga ccgagaaaat ctttaatctg catggtaccg
     4141 atggtgatcc cgatacccga ggtgaaacct aaggtgacgg aaaccggaat atactcaatc
     4201 agacgaccaa agcgtgccag ccccatcaga atcaaaaaga ttcccgacaa caaggtcgca
     4261 accagcagtc ctgccagtcc aaactgttgc gaaacgggat agagaattac cacaaatgcc
     4321 gcagtcggac cggaaacgct aaagcgtgac ccacccgtta gagcaatgac aatccccgca
     4381 acagctgcgg tatataaacc gtactggggg gccacaccac taccaatagc caacgccatc
     4441 gccagcggga tagcaataat cccgacggtt atcccggcaa tcaggtcacg ggtaaaccgt
     4501 gcggcagtat atttttcttt ccagcaaacg tcgatcagag cgcggaaagg catcacatgt
     4561 gaggaaaata ttttgttcac aataatgttt catccgtgag cgcatcatct gtcaactaaa
     4621 tggcaggtga aggaggcata ggtcatacaa atggatatta cagacaaaaa aacccgccgc
     4681 agcgggtctt tgagccgggt tcgattagtg ttcgaacatg gcagagatcg attcttcgtt
     4741 gctgatacga cgaatcgctt cggccagcat acctgacagg gtcagagtac gcacgttcgg
     4801 cagtgatttg atttcatcgc tcagcggaat ggtatcgcag acaacgactt catcaattac
     4861 agagttacgc aggttgttcg ccgcgttgcc agagaagatc gggtgagtcg cgtacgcaaa
     4921 tacacgttta gcaccacgtt ctttcagcgc ttcagcagct ttacacagcg taccgccagt
     4981 gtcgatcata tcatcgacca gtacgcagtc acgacctgca acgtcaccga tgatatgcat
     5041 cacctgggaa acgttcgcac gcggacgacg tttgtcgatg attgccatat cggtatcgtt
     5101 cagcagctta gcgatagcgc gggcacgcac aacgccgccg atgtccggag aaaccacaat
     5161 tgggttatcc agattcagct gcagcatgtc ttccagcagg atcgggctac caaatacgtt
     5221 atcaaccgga acgtcgaaga aaccctgaat ctgttcagcg tgcagatcca ctgtcagcac
     5281 acggtcaaca ccgacgctgg agaggaagtc tgcaaccact ttcgcagtga ttggtacacg
     5341 agcggaacgg acgcgacggt cctggcgcgc atagccaaag taggggataa cagcggtgat
     5401 acgacctgcg gaagcacgac gcagggcatc aaccataacg actaattcca tcaggttgtc
     5461 gttagtaggg gcacaagtgg actggatgat gaaaatatca ccaccgcgta cattttcgtt
     5521 aatttgtacg ctgacttcgc cgtcgctaaa gcgacctaca gcggcgtcgc cgagtgaagt
     5581 gtacaggcgg ttggcaatac gttgtgctag taccggggtg gcgttaccag caaaaagctt
     5641 catatcaggc acgagaagaa cctcaggcat gcgtccattg gtggaaagaa tctgccgaaa
     5701 actgtgcggg ccaggcatga tcctttccag gcggtgtatt aaagagcgcg atgcaacgtc
     5761 tggaacaagg tgacgttgtc accgaaactc agcttgcccg gcttaaagca tggctctgtg
     5821 caatggggaa agattaacgc ctttcgccac aaagccattg agccattccg gggcttgctc
     5881 tagcacctgg cgggcttcag actctgtatc aaattcagca aagacacagg cccctgtccc
     5941 agtcaggcgt gacggggcgt attctaacag ccaggaaagc accgcatcaa cctcgcgaaa
     6001 acgttttctt gcgataacct cgcaatcatt gctgaattca cattttagca acgtttctat
     6061 tgaccttttt ggcgtattgc gcgggagttc aggatcttta aaaatcaccg gagtcggaat
     6121 acttacacca gggtgcgcca ccagatacca cttctctggc ggatccaccg gcgttagtat
     6181 ttcaccaacg ccttcggcaa acgcggcatg cccccgaaca aagacaggaa catctgcgcc
     6241 cagcgtcagc cccatttccg ccagctcatc catgcttagc ccgcattgcc agagatgatt
     6301 taatgccacc aggaccgtcg cggcattgga tgaaccaccg ccgagaccgc cgcccatcgg
     6361 caaacgcttg tcaatgctga tattcgcacc gcttcccgtc ggaagacgcc cgctgtctgc
     6421 cgcagttttc atcaacaacc gcgctgcgcg aacgatcagg ttatcttcat gttccacgcc
     6481 ttcaacgggc gttaacagac gaatatcccc atcgtcacga agctcaatgc tgatggtgtc
     6541 gccgtaatca agaaactgaa acagcgtttg cagcgtgtgg taaccatccg cacgctgacc
     6601 ggtaatgtat aaaaacagat taagttttgc cggagagggc cactgtgtcc gcattatttc
     6661 actatccagt tatccatttt taacttgatg cgttgaccac cgtcggtgag ttccatattg
     6721 gctggcatcg caggttgcgt tttggtgtca taaccaccat aaacaacctt ccagtttttg
     6781 ccattctggc tgtaggtaat ttcgctcagg cggtactggt cgtccagttt gtagtcggtt
     6841 gcatcacccg gtaaacctaa aatccactgg cgcaagctgt tgagcggaat tggcattccg
     6901 gtcaatttgc caatcatctc ttcggcgtca tcggcggtat aacgctgacc tttattgtcg
     6961 actaactgca cgttacccgg ttgagcattc agctccagtt ccgtgctgcc caatgggtta
     7021 gtgagcagca gacggtagcg atcctggccg gtttgctgcc agaaaaagcg ggcgtacact
     7081 ttttgttggt cagaaatata agcgaacgcg ccgcgagtct gatactgatt aagattgcgc
     7141 acgtcttgct gatgctgacg ccattgtggc gaatccgggc ttttgccagg acctttgggc
     7201 gtggtaacgg aacaggcagt gagcacaaga gcagccagcg gtagcaggcg gataagacga
     7261 aaatcgggca ggggcatagt gatgacaagt ccttgagata cgttgcagtt ataaccctta
     7321 atgctagcgt taccgtccgc tatcgtctat gttcaagttg tcttaattgc cagaatctaa
     7381 cggctttcgg caattactcc aaaagggggc gctctctttt attgatctta cgcatcctgt
     7441 atgatgcaag cagactaacc ctatcaacgt tggtattatt tcccgcagac atgacccttt
     7501 tagcactcgg tatcaaccat aaaacggcac ctgtatcgct gcgagaacgt gtatcgtttt
     7561 cgccggataa gctcgatcag gcgcttgaca gcctgcttgc gcagccgatg gtgcagggcg
     7621 gcgtggtgct gtcgacgtgc aaccgcacgg aactttatct tagcgttgaa gagcaggata
     7681 acctgcaaga ggcgttaatc cgctggcttt gcgattatca caatcttaat gaagaagatc
     7741 tgcgtaaaag cctctactgg catcaggata acgacgcggt tagccattta atgcgtgttg
     7801 ccagcggcct ggattcactg gttcttgggg agccgcagat cctcggtcag gttaaaaaag
     7861 cgtttgtcga ttcgcaaaaa ggtcatatga aggccagcga actggaacgc atgttccaga
     7921 aatctttctc tgtagcgaaa cgcgttcgca ctgaaacaga tatcggtgcc agtgctgtgt
     7981 ctgtcgcttt tgcggcttgt acgctggcgc ggcagatctt tgaatcgctc tctacggtca
     8041 cagtgttgct ggtaggcgcg ggcgaaacca tcgagctggt agcgcgtcat ctgcgcgaac
     8101 ataaagtaca gaagatgatt atcgccaacc gcactcgcga acgtgcccaa attctggcag
     8161 atgaagttgg cgcggaagtg attgccctga gtgagatcga cgaacgtctg cgcgaagccg
     8221 atatcatcat cagttccacc gccagcccgt taccgattat cgggaaaggc atggtggagc
     8281 gcgcattaaa aagtcgtcgt aaccaaccaa tgctgttggt ggatattgcc gttccgcgcg
     8341 atgttgagcc ggaagttggc aaactggcga atgcttatct ttatagtgtg gacgatctgc
     8401 aaagcatcat ttcgcacaac ctggcgcagc gtaaagccgc agcggttgag gcggaaacta
     8461 ttgtcgctca ggaaaccagc gaatttatgg cgtggctgcg agcacaaagc gccagcgaaa
     8521 ccattcgcga gtatcgcagc caggcagagc acgttcgcga tgagttaacc gccaaagcgt
     8581 tagcggccct tgagcagggc ggcgacgcgc aagccattat gcaggatctg gcatggaaac
     8641 tgactaaccg cttgatccat gcgccaacga aatcacttca acaggccgcc cgtgacgggg
     8701 ataacgaacg cctgaatatt ctgcgcgaca gcctcgggct ggagtagcag tacatcattt
     8761 tcttttttta cagggtgcat ttacgcctat gaagccttct atcgttgcca aactggaagc
     8821 cctgcatgaa cgccatgaag aagttcaggc gttgctgggt gacgcgcaaa ctatcgccga
     8881 ccaggaacgt tttcgcgcat tatcacgcga atatgcgcag ttaagtgatg tttcgcgctg
     8941 ttttaccgac tggcaacagg ttcaggaaga tatcgaaacc gcacagatga tgctcgacga
     9001 tcctgaaatg cgtgagatgg cgcaggatga actgcgcgaa gctaaagaaa aaagcgagca
     9061 actggaacag caattacagg ttctattact accaaaagat cctgatgacg aacgtaacgc
     9121 cttcctcgaa gtccgtgccg gaaccggcgg cgacgaagca gcgctgtttg caggcgatct
     9181 gttccgtatg tacagccgtt acgccgaagc ccgccgctgg cgggtagaaa tcatgagcgc
     9241 cagcgagggt gaacatggtg gttataaaga gatcatcgcc aaaattagcg gtgatggtgt
     9301 gtatggtcgt ctgaaatttg aatccggcgg tcatcgcgtg caacgtgttc ctgctacgga
     9361 atcgcagggt cgtattcata cttctgcttg taccgttgcg gtaatgccag aactgcctga
     9421 cgcagaactg ccggacatca acccagcaga tttacgcatt gatactttcc gctcgtcagg
     9481 ggcgggtggt cagcacgtta acaccaccga ttcggcaatt cgtattactc acttgccgac
     9541 cgggattgtt gttgaatgtc aggacgaacg ttcacaacat aaaaacaaag cgaaggcgct
     9601 ttcagtgctc ggtgctcgca tccacgctgc tgaaatggca aaacgccaac aggccgaagc
     9661 gtctacccgt cgtaacctgc tggggagtgg cgatcgcagc gaccgtaacc gtacttacaa
     9721 tttcccgcag gggcgcgtta ccgatcaccg catcaacctg acgctctacc gcctggatga
     9781 agtgatggaa ggtaagctgg atatgctgat tgaaccgatt atccaggaac atcaggccga
     9841 ccaactggcg gcgttgtccg agcaggaata atggaatatc aacactggtt acgtgaagca
     9901 ataagccaac ttcaggcgag cgaaagcccg cggcgtgatg ctgaaatcct gctggagcat
     9961 gttaccggca gagggcgtac ttttattctc gcctttggtg aaacgcagct gactgacgaa
    10021 caatgtcagc aacttgatgc gctactgaca cgtcgtcgcg atggtgaacc cattgctcat
    10081 ttaaccgggg tgcgagaatt ctggtcgttg ccgttatttg tttcgccagc gaccttaatt
    10141 ccgcgcccgg atacggagtg tctggtggag caggcactgg cgcggttgcc tgaacaacct
    10201 tgccgtattc tcgatctcgg gacgggtacc ggggcgattg cgcttgcgct ggctagcgag
    10261 cgcccggact gcgaaattat cgctgtagat cgtatgcctg atgctgtctc cctggcacaa
    10321 cgtaatgccc agcatctggc gatcaaaaat atccacattc tgcaaagcga ctggtttagc
    10381 gcgctagccg ggcagcagtt tgcgatgatt gtcagcaatc cgccgtatat tgacgagcag
    10441 gacccacatc ttcaacaagg cgatgtccgc tttgagccgc tcactgcgct ggttgcggca
    10501 gacagtggaa tggcagacat cgtgcatatc atcgaacagt cgcgtaacgc gctggtatcc
    10561 ggcggctttc tgcttctgga acatggctgg cagcagggcg aagcggtgcg acaagcattt
    10621 atcctcgcgg ggtatcatga cgtcgaaacc tgccgtgact atggtgataa cgagcgcgta
    10681 acgctcggcc gctattatca atgacaagtt tttctacact gcttagtgtt catcttatta
    10741 gtatcgcgct ttctgttggg ctattaacct tacgtttctg gctacgttat cagaagcatc
    10801 cacaggcatt tgctcgctgg acgcgcattg tgccgccggt tgtcgatacg gtgttattgt
    10861 taagcggcat tgcgttgatg gctaaagcgc acatcctgcc attttccggg caggcacagt
    10921 ggctgactga aaagctgttt ggagttatca tttatatcgt tttgggtttt attgcactcg
    10981 attatcgtcg tatgcacagt cagcaggcgc gcattattgc cttcccgctg gcgttggtgg
    11041 tgctgtacat catcattaaa ctcgccacca caaaagtacc gttactgggg taagtcatga
    11101 gatcgttagc tgatttcgaa tttaataaag cgccattgtg cgaaggcatg atcctggctt
    11161 gcgaagcaat ccgccgcgat tttccctcgc aagatgttta cgacgaactg gagcgtctcg
    11221 ttagtctggc gaaggaagaa atcagccagc ttctgccttt agaagagcag ctggaaaaac
    11281 tgatcgcgct gttttacggc gactggggat ttaaagcctc acgcggcgtt tatcgtcttt
    11341 ccgatgcatt atggctggac caggtgttaa agaatcgaca gggcagtgcg gtatcattag
    11401 gtgcggtttt attatgggtc gcgaatcgtc tcgatttgcc gctgctgccg gtgattttcc
    11461 ctacgcagct gatattgcgc attgaatgtc cggatggcga aatttggctg attaatcctt
    11521 ttaacggtga atcgttaagc gaacatatgc tggacggatg gttaaaggga aatatcagcc
    11581 cgtcggcgga actgttttat gaagaccttg atgaagctga taacattgag gtaatccgca
    11641 aattgctgga tacactcaaa gcctcgttga tggaagaaaa tcagatggag ctggcgttac
    11701 gcaccagcga agctttatta caattcaacc ctgaagatcc ctatgaaatt cgcgatcgtg
    11761 ggttgattta tgcgcaactg gattgcgaac acgttgcgtt gaacgattta agttatttcg
    11821 ttgaacggtg tccggaagac ccgatcagcg aaatgatccg tgcgcaaata aataacatcg
    11881 cgcataaaca tattgtgctg cattaattaa tcgacatttt actcaagatt aaggcgatcc
    11941 tatgaaacaa aaagtggtta gcattggcga catcaacgta gcaaatgacc tgccgttcgt
    12001 actgtttggc ggtatgaacg tgctggaatc tcgcgatctg gcgatgcgca tttgcgagca
    12061 ctacgtaact gtgacccaga aactgggtat cccttacgtg ttcaaagcct cttttgacaa
    12121 agccaaccgc tcctccatcc actcttatcg tggaccgggc ctggaagaag ggatgaaaat
    12181 cttccaggag ttgaagcaga cttttggcgt gaaaattatc accgacgttc acgaaccaag
    12241 tcaggcacag cccgttgctg atgtcgtgga tgtgattcag ttgccggcgt ttcttgctcg
    12301 ccagactgac ctggttgaag ccatggcgaa aaccggtgcg gtaattaacg tcaagaaacc
    12361 acagtttgtc agcccgggac agatgggtaa tatcgttgat aaattcaaag aaggcggcaa
    12421 cgaaaaagtg attctttgcg atcgtggtgc taacttcggc tatgacaacc tggttgtcga
    12481 tatgctgggc ttcagcatta tgaagaaagt gtctggtaac tcgccggtga ttttcgacgt
    12541 gacccacgca ctgcaatgcc gcgatccgtt tggcgcagct tccggtggtc gtcgtgctca
    12601 ggtggctgag ctggcacgag ccggtatggc ggtaggtctg gcggggctgt ttattgaagc
    12661 gcatccggat ccggaacatg cgaaatgtga tggtccatcc gcgctgccgc tggctaaact
    12721 ggaaccgttc ctcaagcaga tgaaagcgat tgatgatctg gtgaaaggtt tcgaagaact
    12781 ggataccagc aagtaatctt ttttgcttga aaaataaagt cttagcgttc tgcgttaaga
    12841 cttttttcat gggtgccaga tacaaaaaag gccgcaggct gttacccctg cggccggttt
    12901 cgggcgcata ttgccatcac ggcagcctga cgcccgtttt caccttactt ccggttacgc
    12961 caccagctga caatcgctgc ggcaataatc cccgccagga tcggtgctgc caggtcgtgc
    13021 cagaaagtca tggcaaactg cgcgagcgtc atatagccgc cttgttgtaa tgacaacatt
    13081 ttgcggctat tcttgaagtg tctggtttca agattagccc ccgttttgtt gtcaggtttt
    13141 acctctcaac gtgcgggggt tttctctttc cagcaaccaa tgccaccagg gataaagccc
    13201 ccgcaacatt gcgcctcacc ggataattcc ggcttggtgt ggatactacg tctcaattca
    13261 tcttcacttc atccctgaaa tgtttgcaat aaagagtaca ttccggcttt tcaacagctg
    13321 ttgcagtggt ttcgtgcagg ttgtggtaca ggctcgcaat tctgattaac gagctgtcca
    13381 ggtagtatga agaaatacaa taagcaggaa tttatcttat ctctcccgat ttccgtcatc
    13441 ggaggagata taactgtcag gcaaatatcg tcatcaaata ggcggcaaac agtgccagat
    13501 gcgctgcgcc attgagcacg ttagtacgtc cggtggagaa ggagatatgg cacagcacta
    13561 aagaggccac catcaccacc atttctggcg caccaagtgc aaactgcaat tcgttacccg
    13621 tcataaaggc aattagcgtg acgacaggta cggtaagcga aattgttgct aacactgaac
    13681 caaagaacag attcatcgcg cgctgaacct ggttgttcaa cactgctttt aacgcaccta
    13741 aaccttccgg cgacagaatc aacagtgcca ccaggaagcc agtaaaggca acaggggcat
    13801 tcatgctgtc gagcaatgtc tccagcgagc tggcgttcat tttggtgact gcaataacgg
    13861 caatcagatg gataatcaac cagatagcat gccacaggct gctatgggca gacgttttac
    13921 cgtggtgcgg gtcgtcgtca tcactgtcat cttcgtgctc gtagacaaac aaactttgat
    13981 gcgttttggc ctggatcagc aaaaatacgc catacattgc cgcagaaatt aatgctacca
    14041 gtaacgcctg accggttgaa aaattcgccg caggcagagc cattggaaat accagtacga
    14101 ttatcgccag ggggaacagc gcaattaaat actgcttgat accaaacaga ttcatatatt
    14161 gggtggcaaa cttacgaccg cccaacaata atgaaaagcc aaccagccca ccggtaacaa
    14221 tcataatgat tgaatagagc gtatcacgca ttagcgttgg cgcggcgtcg ccggttgcca
    14281 ttaaagctga aatcaaactg acttcaagaa taaccactga aaggctaaga ataagcgaac
    14341 cgtaaggttc tcccaggcga tgggctaata cgtccgcatg acggacaaca ctaaaggcgc
    14401 tacttaaaat accaataagc gcaagaagat tgatggcaat gaccactggt agtgtctggc
    14461 tacttcccca caggaacagc actaccagca ccagaaccgg gaaaataagc gaagtctcct
    14521 tgtggcgggt ttttaccgcc tcttgagcat ttgacattat ggttatccct ttgcagatga
    14581 atttatcgaa aatgtaaaaa ataggtagga aaaataacag aaagtgtctg gatatcggta
    14641 acattttacg aatttttacc ccgctgtcga ttttttactc attggggcat aaaaataagt
    14701 attacgttta gacaatgttt gttttagcgt ttcttaagaa cagtctgaca tgaaaattct
    14761 tatgttttgg caagagtaga tattgttgac cacacttaat gttcaacttt gtaaaaggag
    14821 tcaacgatgc cgtataaaac gaaaagcgat ctgccggaaa gcgtaaagca cgttctaccg
    14881 tctcatgccc aggatatcta taaagaagcg ttcaacagcg catgggatca atataaagat
    14941 aaagaagatc ggcgtgatga cgccagtcgc gaagaaaccg cgcataaagt ggcctgggct
    15001 gctgtgaagc atgaatatgc caaaggggat gatgataaat ggcataaaaa atcgtaaaac
    15061 cggtagctta gttaaagcta ttcgtgcggt gttgccttgc aagtggtccg tggattgcat
    15121 attgttccgt tagtggtttc aaaatgagcg gtaaaaatgt ccggaagaca ccaaaaagtt
    15181 gtcgcaggga agtatgcagt ggcggaggtg taaggtgata acgcgtgatt tcttgatgaa
    15241 tgccgattgt aaaacggcat ttggtgccat tgaagaatca ctcttatggt cagcagaaca
    15301 acgggcggct tcgctggcgg cgacgctggc ttgtcgacct gatgagggac cggtgtggat
    15361 cttcggttat ggatcgttga tgtggaatcc ggcactggag tttaccgaat catgcaccgg
    15421 tacactggtt ggatggcatc gcgcgttttg cctgcgcctg accgccgggc gcgggactgc
    15481 gcaccagccg ggacggatgc ttgcactgaa agagggcgga cgcaccacag gcgtcgccta
    15541 tcgactacca gaagagacgc tggagcagga actaaccctg ttgtggaagc gcgagatgat
    15601 taccggctgt tatctgccaa cctggtgtca gcttgatctt gatgatggac gcacagtaaa
    15661 cgccattgtg tttattatgg acccgcgaca tccagaatat gaatctgaca ctcgcgctca
    15721 ggtcatcgcg ccgttgattg cggcggcgag cggtccgctg ggaaccaatg cacaatacct
    15781 gttttcactg gaacaggagc ttatcaaact gggaatgcag gacgatgggc tgaatgattt
    15841 gctggtatcg gtaaaaaaac tactggcgga gaattatccg gatggtgtgt taaggccggg
    15901 attcgcctga gtaaacttcc ctcatagtgg ggcgtcagac gcccccttaa acattaaaat
    15961 gtgagcactt tatcggctga cagcgtccat tgcgccagtt ccacaagagt accgatttcc
    16021 accccatcaa tcagaggaag tgtgctaatc ccgcgcccgt cggtacaggt tttgcacaat
    16081 ttcaccggta cattctgagc ggtaaggatc tccagcattt gctgaatgtt gtagccttcc
    16141 cctggttttt gcccgcgcaa cccggctgtg accgcatcag acatcaggaa cagacgcaga
    16201 tccagattgc tctcctgctc tcgtaacgca atggccagcc gcaagctgtt aaacaaggat
    16261 tcgctcccgt aaggtgcgcc attggcaacg atcactattt tttgcattat ttactcctgt
    16321 attcagggaa ttagacactc atcttctatc ttactgcttc tgcagcgtct gaccaatcgg
    16381 tcacattttt aaggattttc ctgaaagcgc gagaaaatac gacaaaagtt gccagtaatc
    16441 gttattcttt aaggctatgg tttttcatta ttaccggaag ttaccgacgt tttgagccgt
    16501 ttcgttcctc gcattatccc gttttattta ctcttgcttg tagcaggcgg tacagctaac
    16561 gcacaatcta ccttcgagca aaaagcggca aatccctttg ataataacaa tgatggtctg
    16621 ccggatttag gcatggctcc cgaaaatcat gatggggaaa aacactttgc tgaaattgtg
    16681 aaagatttcg gcgaaaccag tatgaatgat aacgggctgg atactggcga gcaggcaaaa
    16741 gctttcgcat tgggaaaagt ccgcgacgcg cttagtcaac aggttaatca gcacgtagag
    16801 tcctggctat caccgtgggg aaatgccagt gttgacgtca aagtggataa cgaaggacat
    16861 ttcaccggca gtcgtggaag ctggtttgtg ccgttacaag ataatgatcg ttatctcacc
    16921 tggagccagc ttggtcttac tcagcaggat aatgggttgg tgagcaatgt gggcgttggg
    16981 caacgctggg cgcgcggcaa ctggctggtg ggttataaca ctttttatga caacttgcag
    17041 gacgaaaatc ttcagcgagc gggctttggt gccgaagcgt ggggcgaata tttgcgatta
    17101 tcggcaaact tttatcagcc gtttgctgca tggcatgaac agacagccac gcaggaacaa
    17161 cggatggcgc gcgggtacga cctgacagct cggatgcgca tgccgttcta tcaacacctc
    17221 aataccagtg tcagcctaga acagtatttt ggtgatcgtg ttgatttgtt taactctggt
    17281 acgggttatc acaatcccgt cgcgttgagt ctgggattaa attacacccc tgtgccatta
    17341 gtcactgtga cggcccagca taaacagggg gaaagtggcg aaaatcaaaa taacctcggg
    17401 ctgaatctta attaccgctt tggtgtaccg ctcaaaaaac aactttctgc gggcgaggtt
    17461 gccgaaagtc agtcgttacg tggtagtcgc tatgacaatc cgcagcgaaa taatctaccg
    17521 actcttgagt accgacagcg aaaaacgtta acggtgtttc tggcgacacc gccgtgggat
    17581 ctaaaacctg gcgaaacagt gccgctgaaa ttacaaatcc gcagtcgtta cggtattcgg
    17641 caactgattt ggcagggcga tacgcagata ttaagtttga cgccgggcgc acaagccaac
    17701 agcgcggagg gctggacgct gatcatgcct gactggcaga acggggaagg ggcaagcaat
    17761 cactggcgat tgtctgtggt ggtggaagat aaccaggggc agcgtgtctc ctccaatgag
    17821 atcacgctaa cgcttgtcga accgttcgat gcattgtcaa acgacgaact gcgctgggaa
    17881 ccgtaatcag aaaatgcgct cctgatgcac ccataccgct gcttccacgc gagacttgag
    17941 cttcattttc ttcagcatgt gcttgacgtg cacttttact gtgctttcgg tgatatccag
    18001 gcggcgggca atcatcttgt tcggcaaacc ctgggcaatc agcttgagaa tatcgcgctc
    18061 gcgtggggtt aactggttaa catcgcgctc agtagtggca cggttagcgc gcaagctggc
    18121 ggccagaaca ggcgttaatg cttcgcttaa taccatttcg ccagcagctg cctgatgcaa
    18181 tgctttcagc agatcttccg gttccatatc ttttaacaga tagccatccg cgccgcgttt
    18241 cagtgcggtg accacatctt cttcatggtt agagacgctg aataccacaa tgcgccctga
    18301 gagggacttt tcgcgcagtt tatccagcgt ttccagaccg ttcatgccgg gcatattgag
    18361 atctaacagg atcagatcgg gatcaagaga ttccgccagt tcaataccct gttcgccatt
    18421 actcgcttcg ccaaccacgg tgatatctgg tgccatactg ataagctgtt ttacgccagt
    18481 tcgcagcatc gggtgatcgt caatcagcag gatagtagcc ggttcctgat tactcatggg
    18541 tatctccttg gacgtctgtg aaagtttttt cgggaataaa ggtgaccacc acttcggtgc
    18601 cacctgattc acgacggcgg acgcggcaat cgcctcgtaa actttgcgca cggtcgcgca
    18661 ttattatcat gccgtagtga ttgctgcgga tggcattttc aggcacgcca cagccgttat
    18721 cctggacggt cagtttgacc tgattatcgt tttgcgccac cgtcaccacg acctcactcg
    18781 cttgcgaatg tttgagggcg ttacttaatg cctcacgggc aatttgcaac aagtggattg
    18841 cctgatgtga aggcaccaga cgaggcggca attgataatc cagcttcacc gggaagccaa
    18901 atttggcgct gtactcttcg caactcgctt ccagcgccgg acgtaatcca ggctcggtga
    18961 gctgcaagcg gaatgtggtg agcaattcac gcaactgcgc ccaggatgca ttcagttcgt
    19021 tacggatctg acttaacagt tcgcggctgc tttctggcag cgcatcgccc tgcatctgta
    19081 aacaactcac ctgcatcttc atgcaagaga gagattgggc aatagaatca tgcagttcgc
    19141 gcgcaatggt ggcacgctct tccatcacga tcaactgttg ctgacgttcc tgatggcgat
    19201 ccagcgccag cgtggcggta agttgttcaa ccagggtatc caccagttgt tgttggtcat
    19261 ggctaagatg acgcccctgc ggcagggtcg ccagcaaaat accgtactgc gtatgagagt
    19321 cagccagccg ccacttcagg gtcgtgccgc gatcaccaac gggtaatacg ccgcgcgggc
    19381 agagctggca gcctttatca tcacaagtca tatctggctg gcaggtaaac tcctgatgat
    19441 tctcttcatc atcagtgtca tacacccgca gttcgatatc acgtagcagg gttaaattct
    19501 gtaagccgtt gagtaccggt gacaggcgtt cacacagcgg ggcgcgggaa tgcaaacggc
    19561 ggttagcctg ccataaaaaa gagaggatct gatttttatg ctccagcccg gcggttttct
    19621 cctgaacccg ctgctcaagt acggcataac tttcggccag ttctgcagac atattgttca
    19681 acgcagttcc aagcatcgcc atttcgttgc gcccgctgat gtttgcgcgt tgggtaaaat
    19741 cgcgatggct gacggcactc gccattgcca gcagttgccg ccacggttgt agcagtcgcg
    19801 cccgcaacca gataatagtg aacaccagta aaagtgccat aaataccgcc attacccgat
    19861 ggaccagtac cactgtctca atacgcattt ccgtggtgcg gtcaaaacca gataccagct
    19921 gatcaagccc ggcaacaaac tggctgacat ccgctgacac cgtttctcgg ttttgtgcac
    19981 gcatcagcgc agggatcagt tcattacgcc agtaatcttg taagccctgt aattgcgcca
    20041 gttgtccgtc tcgttctgct gctcgagtca actcggcgct aaatgccgtt tgttccatct
    20101 ctttaattaa gggcttgtct ttctcgctta atggcactgc cgccaacaga cggtaacttt
    20161 gcatgcgcag cgatcccgct ttgttgatcg catgggcgct gccctgaacg ccttgcacca
    20221 gccagccaga aaccgccatc cctgccagtc caatagcagt agaaagcaac acaataagcg
    20281 caacctgatt aaccagggtg agcggagaga gacaacgttt aagcatgtaa accccttcct
    20341 tcaggcttta aatgagcaat aaccttaatg aatgtgacga tatattctgg aatggcagta
    20401 ttctcggcta ttggctgaag tatacccata gccggaaaga gttactcctt atttgccgtg
    20461 tggttagtcg ctttacatcg gtaagggtag ggattctaca gcaccgtgaa aaatctcata
    20521 atttttatga aatcactgta ctcactatgg gtaatgataa atatcaatga tagataaagt
    20581 tatcttatcg tttgatttac atcaaattgc ctttagctcc agacactaag gtggcagaca
    20641 tcgaaacgag tatcagaggt gtctatgagt cactcatctg cctccgaaag ggctactgga
    20701 gctgtcataa cagattggcg accggaagat cctgcgttct ggcaacaacg cggtcaacgt
    20761 attgccagcc gcaacctgtg gatttccgtt ccctgtctgc tgctggcgtt ttgcgtatgg
    20821 atgttgttca gcgctgttgc ggtgaactta ccgaaagtcg gttttaattt tacgaccgat
    20881 cagctattta tgttgactgc gctgccttcg gtttctggcg cgttattacg tgttccatac
    20941 tcctttatgg ttcctatctt cggtggtcgt cgctggacgg cgttcagcac cggtattctg
    21001 attattcctt gcgtctggct gggttttgcc gtgcaggata cctccacgcc ttatagcgtc
    21061 ttcatcatca tctctctgct atgcggcttt gctggcgcga acttcgcatc cagtatggca
    21121 aacatcagct tcttctttcc gaaacagaag cagggtggcg cgctgggtct gaatggtggt
    21181 ctgggaaaca tgggcgtcag cgtcatgcag ttggttgctc cgctggtggt atcactgtcg
    21241 attttcgcag tatttggtag ccagggcgtc aaacagccgg atgggactga gctgtatctg
    21301 gcgaatgcgt cctggatatg ggtgccgttc cttgccatct tcaccattgc ggcgtggttt
    21361 ggcatgaacg atcttgctac ctcgaaagcc tccatcaagg agcagttgcc ggtactcaaa
    21421 cggggtcatc tgtggattat gagcctgctg tatctggcaa ccttcggctc cttcatcggc
    21481 ttctccgcgg gctttgcgat gctgtcaaaa acgcagttcc cggatgttca gattctgcaa
    21541 tacgctttct tcgggccgtt tattggtgcg ctggcgcgtt ctgcaggtgg tgcattatct
    21601 gaccgtctgg gcggaactcg tgtcacgctg gtgaacttta ttctgatggc gattttcagc
    21661 ggcctgctgt tcctgacctt accgactgac gggcagggcg gaagcttcat ggcgttcttc
    21721 gcggtcttcc tggcgctgtt cctgacagct gggctgggta gtggttccac tttccagatg
    21781 atttcagtga tcttccgtaa actgacaatg gatcgcgtga aagcagaagg gggttctgac
    21841 gaacgtgcga tgcgtgaagc ggcaaccggc acggcagcgg cgctgggttt catctctgcg
    21901 attggcgcga ttggtggctt ctttatcccg aaagcgtttg gtagctcgct ggcattaacg
    21961 ggttcgccag taggtgcaat gaaagtattt ttgattttct atatcgcctg cgta
//
LOCUS       DAZPEU010000054        21493 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPEU010000054 DAZPEU010000000
VERSION     DAZPEU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.054"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            86..1600
                     /gene="caiT"
                     /locus_tag="ACHU2L_001844"
     CDS             86..1600
                     /gene="caiT"
                     /locus_tag="ACHU2L_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR1356722.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1631..2773
                     /gene="caiA"
                     /locus_tag="ACHU2L_001845"
     CDS             1631..2773
                     /gene="caiA"
                     /locus_tag="ACHU2L_001845"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR1356723.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2902..4119
                     /gene="caiB"
                     /locus_tag="ACHU2L_001846"
     CDS             2902..4119
                     /gene="caiB"
                     /locus_tag="ACHU2L_001846"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR1356724.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4193..5746
                     /gene="caiC"
                     /locus_tag="ACHU2L_001847"
     CDS             4193..5746
                     /gene="caiC"
                     /locus_tag="ACHU2L_001847"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR1356725.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5855..6640
                     /gene="caiD"
                     /locus_tag="ACHU2L_001848"
     CDS             5855..6640
                     /gene="caiD"
                     /locus_tag="ACHU2L_001848"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR1356726.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6646..7236
                     /gene="caiE"
                     /locus_tag="ACHU2L_001849"
     CDS             6646..7236
                     /gene="caiE"
                     /locus_tag="ACHU2L_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR1356727.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7322..7717)
                     /gene="caiF"
                     /locus_tag="ACHU2L_001850"
     CDS             complement(7322..7717)
                     /gene="caiF"
                     /locus_tag="ACHU2L_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR1356728.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7979..11200)
                     /gene="carB"
                     /locus_tag="ACHU2L_001851"
     CDS             complement(7979..11200)
                     /gene="carB"
                     /locus_tag="ACHU2L_001851"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR1356729.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11218..12366)
                     /gene="carA"
                     /locus_tag="ACHU2L_001852"
     CDS             complement(11218..12366)
                     /gene="carA"
                     /locus_tag="ACHU2L_001852"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR1356730.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(12822..13643)
                     /gene="dapB"
                     /locus_tag="ACHU2L_001853"
     CDS             complement(12822..13643)
                     /gene="dapB"
                     /locus_tag="ACHU2L_001853"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR1356731.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(13810..14724)
                     /gene="rihC"
                     /locus_tag="ACHU2L_001854"
     CDS             complement(13810..14724)
                     /gene="rihC"
                     /locus_tag="ACHU2L_001854"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR1356732.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(14790..15740)
                     /gene="ispH"
                     /locus_tag="ACHU2L_001855"
                     /gene_synonym="lytB"
     CDS             complement(14790..15740)
                     /gene="ispH"
                     /locus_tag="ACHU2L_001855"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR1356733.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15742..16191)
                     /gene="fkpB"
                     /locus_tag="ACHU2L_001856"
     CDS             complement(15742..16191)
                     /gene="fkpB"
                     /locus_tag="ACHU2L_001856"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1356734.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16278..16772)
                     /gene="lspA"
                     /locus_tag="ACHU2L_001857"
     CDS             complement(16278..16772)
                     /gene="lspA"
                     /locus_tag="ACHU2L_001857"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR1356735.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16772..19588)
                     /gene="ileS"
                     /locus_tag="ACHU2L_001858"
     CDS             complement(16772..19588)
                     /gene="ileS"
                     /locus_tag="ACHU2L_001858"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR1356736.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19631..20572)
                     /gene="ribF"
                     /locus_tag="ACHU2L_001859"
     CDS             complement(19631..20572)
                     /gene="ribF"
                     /locus_tag="ACHU2L_001859"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR1356737.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20580..20798)
                     /locus_tag="ACHU2L_001860"
     CDS             complement(20580..20798)
                     /locus_tag="ACHU2L_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR1356738.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20901..21164
                     /gene="rpsT"
                     /locus_tag="ACHU2L_001861"
     CDS             20901..21164
                     /gene="rpsT"
                     /locus_tag="ACHU2L_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR1356739.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5137 a         5762 c         5514 g         5080 t
ORIGIN      
        1 ttaataataa ttatattaaa tgttaacaaa aataaaacaa acgggaacgc aaaataaata
       61 ttcgttttca cagtggaatt aactcatgaa gaatgaaaag agaaaaacgg gaatagaacc
      121 gaaggttttc tttccgccat taataatcgt cggcatactt tgttggctta cagtcagaga
      181 tctggatgcg gcgaatgtcg ttattaatgc tgtattcagt tacgtcacca atgtatgggg
      241 atgggcattt gaatggtata tggtggtgat gcttttcggt tggttctggc tggtgtttgg
      301 cccgtatgcc aaaaagcgtt taggtaacga accgccagaa tttagcaccg ccagttggat
      361 ctttatgatg ttcgcctcct gtacgtctgc tgccgtactg ttctggggat cgattgagat
      421 ctactactac atctccaccc cgccgtttgg cttagaaccg aactcgacag gagcgaaaga
      481 gttggggctg gcttacagct tgttccactg gggacctctg ccgtgggcca cttacagctt
      541 cctttcagtc gccttcgctt acttcttctt tgtccgcaaa atggaagtga ttcgccccag
      601 ctcgacactg gtgccgctgg caggtgaaaa acacgccaaa gggttgttcg gtaccatcgt
      661 cgacaacttc tatctcgtcg ccttgatctt cgcgatgggt accagtctgg gccttgccac
      721 gccgctggtg accgagtgta tgcaatggtt gtttggcatt ccgcataccc tgcaactgga
      781 cgctatcatc attacctgct ggattatcct caacgccatt tgcgtcgctt gcggtctgca
      841 aaaaggggta cgtatcgcca gtgacgtgcg tagttacctg agcttcctga tgctgggttg
      901 ggtgttcatc gtcagcggtg ccagcttcat catgaactac ttcaccgatt cggtggggat
      961 gttgctgatg tatctgccgc gcatgttgtt ctataccgat cccatcgcta aaggcggctt
     1021 cccgcagggc tggaccgtgt tctactgggc atggtgggtg atttacgcca tccagatgag
     1081 tatcttcctc gcccgcatct cccgtggtcg tactgtgcgt gaactgtgct tcggcatggt
     1141 gatggggctg acagcatcca cctggatcct gtggaccgtg ctcggtagta acactctgct
     1201 gttgatggat aaaaacatca tcaacattcc aaatctgatc gaacagtacg gtgtggcgcg
     1261 cgccatcatc gaaacctggg ccgctctgcc actcagcacc gccaccatgt ggggcttctt
     1321 catcctctgc tttattgcca ccgttacgct ggttaacgcc tgctcttata ccctggcgat
     1381 gtctacttgc cgcgaagtac gcgatggtga agaaccaccg ctgctggtgc gtatcggttg
     1441 gtcaattctg gttggcatta tcggtattgt tctgctggcg ctcggcggcc tgaaaccgat
     1501 tcaaaccgcc attatcgccg gaggatgccc gctgttcttc gtcaacatta tggtgacgct
     1561 ctcctttatt aaagacgcga aacagaactg gaaagattaa ttaaccccca aaatatcaag
     1621 aggttgaaag atggatttta atttaaatga tgagcaggaa ctgtttgtcg ccggtatccg
     1681 cgaactgatg gccagcgaaa actgggaggc ctattttgcc gagtgcgacc gtgacagcgt
     1741 ctacccggaa cgttttgtca aagcactggc ggatatgggt atcgacagtc tgctgatccc
     1801 tgaagagcac ggtggtctgg acgcggggtt tgttactctc gccgccgtgt ggatggagct
     1861 gggacgtctg ggggcaccaa cctatgtact gtaccagctg ccgggcgggt tcaatacctt
     1921 cctgcgcgaa ggcacacaag agcagatcga caagattatg gctttccgcg gcaccggtaa
     1981 gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc tccgacgtgg gtagcctgaa
     2041 aacgacttat acccgtagaa atggtaagat ttatcttaat ggtagtaagt gttttattac
     2101 cagtagcgcc tacaccccgt acatcgtggt gatggcgcgc gacggggctt ctccggacaa
     2161 acctgtctac accgaatggt ttgttgatat gagcaaaccg ggcatcaaag tgaccaaact
     2221 tgagaagctc ggtctgcgta tggatagctg ctgtgaaatc acctttgacg atgtggaact
     2281 ggacgagaaa gacatgttcg gtcgggaagg taacggcttt aaccgcgtca aagaagagtt
     2341 cgaccatgaa cgtttcctgg tagccctgac caactacggt acggcgatgt gcgcctttga
     2401 agatgctgcg cgctacgcca accagcgcgt gcagtttggc gaggctattg gtcgtttcca
     2461 gttgattcag gaaaaattcg cccacatggc gatcaaatta aactccatga aaaacatgct
     2521 gtatgaagca gcgtggaaag cagacaacgg caccatcacc tctggcgatg cagcgatgtg
     2581 caaatacttc tgcgccaatg cggcatttga agtggtggat agcgcaatgc aggtgctggg
     2641 cggtgtcggg attgcgggca accaccgcat cagccgcttc tggcgtgacc tgcgtgtaga
     2701 ccgcgtttcc ggaggatctg acgaaatgca gatcctgacg ctgggtcgtg cggtgctgaa
     2761 gcaataccgc taagtccgtg cgctgcctga tgcgacgctg acgcgtctta tcaggcctac
     2821 gggagcacga atgtaagccg gataaggcgt ttacgccgca tccggcagtc atgcgctaca
     2881 taaatttttc aggagctaat aatggatcat ctacccatgc cgaaattcgg accgctggcc
     2941 ggattgcgcg ttgtcttctc cggtatcgaa atcgccggac cgtttgccgg acaaatgttc
     3001 gcagaatggg gcgcggaagt tatttggatc gagaacgtcg cctgggccga caccattcgc
     3061 gttcaaccga actacccgca gctctcccgc cgcaatttgc acgcgctgtc gttaaatatt
     3121 ttcaaagatg aaggccgcga agcgtttctg aaattaatgg aaaccaccga tatcttcatc
     3181 gaagccagta aaggtccggc ctttgcccgt cgtggcatta ccgatgaagt actgtggcag
     3241 cacaacccga aactggttat cgctcacctg tccggttttg gtcagtacgg caccgaggag
     3301 tacaccaatc ttccggccta taacaccatc gcccaggcct tcagtggtta cctgattcag
     3361 aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata ccgccgatta cttttctggc
     3421 ctgaccgcca ccacggcggc gctggcagca ctgcataaag tgcgtgaaac cggtaaaggc
     3481 gaaagtatcg acatcgccat gtatgaagtg atgctgcgta tgggccagta cttcatgatg
     3541 gattacttca acggcggcga aatgtgcccg cgcatgagca aaggtaaaga tccctactac
     3601 gccggttgcg gtctgtataa atgtgccgac ggctacatcg tgatggaact ggtgggcatt
     3661 acccaaattg aagagtgctt taaagatatt ggcctcgcac atctactcgg tacgccggaa
     3721 atcccggaag gcactcagct tatccaccgt atcgaatgcc cttacggccc actggtagaa
     3781 gaaaaactcg atgcctggct ggcggcacat accatcgcag aagttaaaga acgctttgcc
     3841 gaactgaata tcgcctgtgc caaagtactg accgtaccgg aactggaaag caatccacag
     3901 tatgttgccc gtgaatcaat cactcagtgg caaacgatgg atggtcgcac ctgcaaaggg
     3961 ccgaacatca tgccgaaatt caaaaataac cccggacaaa tctggcgcgg aatgccctca
     4021 catggcatgg acacggctgc cattttgaaa aatatcggct acagcgaaaa cgacattcag
     4081 gagttggtca gcaaaggtct ggccaaagtt gaggactaaa cgcgtggcgt cagtcggctt
     4141 gcctgactag cgcccgccca ataacaaacg aacaaaaatg gatagaggtg caatggatat
     4201 cattggcgga caacatctac gtcaaatgtg ggacgatctg gcggacgttt acggtcataa
     4261 aacggcgctg atttgtgaat ccagcggcgg agtcgttaac cggtatagtt atcttgagtt
     4321 aaatcaggag attaaccgca cggcaaacct gttttatacg ctggggattc gcaaaggcaa
     4381 caaggttgca ctacatctcg acaactgccc ggaatttatc ttttgctggt tcgggctggc
     4441 aaaaattggc gcgattatgg tgccgattaa cgcccgcctg ttacgcgagg aaagcgagtg
     4501 gatcttgcaa aatagccagg cgtgcctgct ggtgaccagt gcgcaatttt atcctatgta
     4561 tcaacagatt cagcaggaag atgccactca attgcggcac atttgcctga cagatgtggc
     4621 acttcccgct gatgatggag tgagttcatt tactcaactg aaaaatcaac aacctgccac
     4681 cttgtgctat gcaccgccgc tatcgactga cgatacggcg gaaattctct tcacctccgg
     4741 caccacctcc cgaccgaaag gtgtggtgat tacccattac aacctgcgct tcgctggata
     4801 ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc tacatgacgg taatgcctgc
     4861 gtttcatatc gattgccagt gtactgcggc gatggcggcg ttttctgccg gggccacctt
     4921 tgtgctggtc gagaaataca gcgcccgcgc cttctgggga caggcgcaga agtaccgcgc
     4981 caccattacc gaatgtattc cgatgatgat ccgtacgttg atggtgcagc cgccttcagc
     5041 gaacgatcgg caacaccgcc tgcgggaagt gatgttttat ctcaacttgt cggagcagga
     5101 aaaagacgca ttttgtgaac gcttcggcgt tcgcttgctg acgtcttatg ggatgacgga
     5161 aaccattgtg ggcattatcg gcgatcgccc tagcgataaa cgacgctggc cgtcgattgg
     5221 tcgggcgggg ttttgctacg aagcggagat ccgcgacgat cacaatcgcc cgctcccggc
     5281 aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct tcaaagagta
     5341 ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc gacggctggc tacataccgg
     5401 cgataccgga taccgcgacg aagaaggctt tttttatttc gtcgatcgcc gctgcaatat
     5461 gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg gaaaatatca tcgccaccca
     5521 tccgaaaatt caggacatcg tggttgtggg tattaaagat tcgattcgcg atgaagccat
     5581 caaagcattt gtggtgctga atgaaggtga aacattgagc gaagaggaat tttttcgctt
     5641 ctgcgaacaa aatatggcga aatttaaagt gccctcttat ctggagatca gaaaagatct
     5701 gccacgtaat tgctcgggga aaataattag aaagaatctg aaataaatga aacgtcaggg
     5761 aactaccctg ccagctaata atcacgccct aaaacaatat gcattttttg cagggatgtt
     5821 gtcgtcccta aaaaagcaaa aatggagaaa aggaatgagt gaatcattac atctgacccg
     5881 caatggatca attctggaaa ttacccttga tcgcccaaaa gcgaatgcta ttgatgcaaa
     5941 aaccagcttt gaaatgggcg aagtatttct aaatttccgt gacgatccgc aattacgtgt
     6001 cgccattatt accggtgccg gagagaaatt cttttccgcg ggctgggatt taaaagcggc
     6061 agcagaaggc gaagcaccgg atgctgactt tggcccgggt ggttttgcgg gattaaccga
     6121 aattttcaat ctcgacaaac cagttatcgc agctgtgaac ggctacgcct ttggcggcgg
     6181 tttcgaactg gcgctggcgg cagattttat tgtttgtgcc gataacgcca gcttcgccct
     6241 gccggaagcc aaactgggca tcgttcctga cagcggcggt gtgctgcgtc tgccgaagat
     6301 cctgccgcct accatcgtca atgaaatggt gatgaccggc agacgaatgg gcgcagaaga
     6361 ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag gcggaactga tggataacgc
     6421 ccgcgaactg gctcagcagc tggttaacag cgccccgctg gcgattgcgg cgctgaaaga
     6481 gatctaccgc accaccagcg aaatgccggt agaagaagcg tatcgctata ttcgcagcgg
     6541 cgtgttgaaa cactatccat cggttctgca ttcggaagat gccattgaag ggccgctggc
     6601 gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa caaccgtgag ctattacgcc
     6661 tttgaggggt taattccggt ggttcacccg acggcgtttg tccatcccag tgccgtcttg
     6721 attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc actgcgtggt
     6781 gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg cattatgcat
     6841 ggctactgcg acactgacac catcgttggg gaaaacggcc atatcgggca tggagcgatc
     6901 ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt aattatggat
     6961 ggcgcggtca ttggcgaaga gagcattgtt gccgcaatga gctttgtcaa agcaggcttt
     7021 agtggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtacg cagtgttagt
     7081 gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct tgtcgggcgc
     7141 tgtcatgcat cgttacatga aacgcagcca ctgaggcaaa tggaggaaaa tcgcccccgt
     7201 ttgcagggga cgacggatgt gacgcctaaa cggtgatctt gccggatgcg acctggttgc
     7261 tggataagat gctacaacat cgtatctggc aagcaggacg cccggtatca acgagataaa
     7321 attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg ttaagttccg
     7381 tgagcggaac ggcaggaata cgcggtgtct ttcttacgcc gactaatttt tcccgactgt
     7441 tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cactttaacc agtcgctgac
     7501 actggcatcc ccgcccttcc agcttattag ggatcatctt aacttcgcag cttatttctg
     7561 tgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca atatcgaaat
     7621 gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat tcggcgatta
     7681 acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt tcacaaatta
     7741 aaagtctagc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa attgtggata
     7801 tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct tatgtcatca
     7861 aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaaacggtc ctcatcagag
     7921 aaccgtatta gttattcgat caaattagcg gatgaaaaat atccgccacg acacgctatt
     7981 atttgatctg tgcgtgcatt tcctgcaccg aaattacttt ttcagtcgca tcggcattca
     8041 gcgccatcgc ggtagcgaaa ccaccgttca gggtggtgtc ataatgcact ttatattgca
     8101 gcgcactgcg acggatcacg cgggagtctt caatcgcacg acggcctgag gtggtgttga
     8161 tgatgtaggt atattcgcca ttcttgatac ggtcctgaat gtgcggacgg ccttcatgca
     8221 ccttgtttac cagacgcgga ttgatacccg cttcgcccag cacaatcgcc gtgccgtggg
     8281 tcgcatccag ctcgaagccc tgtttcagca gttttgccgc caggtccacc acgcgttctt
     8341 tatcgccttc gcgcacggaa agcagcgcac gaccgtgttt cttcatggtg gagttgctgc
     8401 ccagctgcgc tttggcgaac gcttcagcga aggtgcggcc cacgcccatg acttccccgg
     8461 tagagcgcat ttctggccct aacagcgggt caacgcctgg gaatttattg aacggcagca
     8521 ccacttcttt caccgagtag tacggcggaa taacttcttt ggttacgccc tgctcagcca
     8581 gcgatttgcc agccatcaca cgcgccgcca cttttgccag cggtacgccg gtggctttgg
     8641 agacgaacgg aacagtacgc gccgcacgcg ggtttacttc aatcaggtaa acttcgttgt
     8701 ttttcaccgc aaactgcacg ttcatcagac cgcgcacctg caattcgaag gccagtttct
     8761 gcacctgctg gcgcatcaca tcctgaattt cctgacttaa ggtgtacgct ggcagcgaac
     8821 atgcggagtc accggagtgc acacccgcct gctcgatgtg ctccatgatg ccgccaatca
     8881 gcaccatttc gccgtcgcag atggcatcca cgtcaacttc tactgcgtca tcaaggaaat
     8941 gatccagcaa cactggcgca tcgttagaca cgctaaccgc cgtctggaag tagcgacgca
     9001 ggtcagcttc gtcatagacg atttccatcg cccgaccgcc gagaacgtaa gacggacgca
     9061 ccaccagcgg gtagccaatc tcttttgcct tctcaactgc catttcaata gcagtaacgg
     9121 tggcgttcgc cggttgtttc agtttcagac ggtcaaccgc atgctggaag cgttcacggt
     9181 cttccgcacg gtcgatagca tccgggctgg tgccgataac cggtacgcca gcggcttcca
     9241 gtgcacgcgc cagtttcagc ggggtctgac cgccgtactg aacgataacg cctttcggct
     9301 tctcaatacg cacgatttcc agcacatctt ccagagttac cggctcgaag tagaggcgat
     9361 cggaagtgtc gtagtcggta gagacggttt ccgggttaca gttaaccata atggtttcgt
     9421 aaccgtcttc acgcagcgcc agcgaggcgt gtacgcagca gtagtcgaat tcgataccct
     9481 gaccgatacg gtttggaccg ccgccaagca ccatgatttt ttcacggtcg gtagacggat
     9541 tcgcttcgca ctcttcttca taagtggagt acatgtaagc ggtgtcggtg gcaaactctg
     9601 ccgcacaggt atccacgcgc ttgtagaccg ggtgcaggtc atattggtca cgcagcttac
     9661 ggatttccgc ttcgcgtacg cccgccagtt ttgccaagcg cgcatcggca aagcctttgc
     9721 gtttcagctg gcgcaggaat tcagcgttca ggccagtgat gcccacttcc gccactttct
     9781 cttccagacg caccagttct tcaatctgta ccaggaacca gcggtcaatg ttagtcaggt
     9841 tgaagacgcc gtccacagac aggcccgcgc ggaaagcatc ggcgatgtac cagatacgct
     9901 ctgcgcctgc gtctttcagt tcgcgacgga ttttggttaa cgcttccggg tcatccaggc
     9961 tcactttcgg gtcgaagcca gttgcgccca cttccaggcc gcgcagcgct ttttgcaggg
    10021 attcctgctg cgtgcgacca atcgccatca cttcgccaac cgatttcatc tgagtggtca
    10081 gacggtcgtt agcaccggcg aatttttcga agttgaagcg aggaatttta gtaaccacat
    10141 agtcgatgga cggctcgaag gaggccggag tacgtccgcc agtgatgtcg ttcatcagtt
    10201 cgtctagggt gtaacccacc gccagtttcg ccgccacttt agcaatcggg aaaccggtcg
    10261 ctttcgacgc cagcgccgaa gaacgggaca cgcgtgggtt catttcgata acaatcaggc
    10321 gaccgttttt cgggttcacc gcaaactgga cattggaacc accggtttca acgccgattt
    10381 cacgcagcac cgccatcgag gcgttacgca tgatttgata ttctttgtcg gtcagcgttt
    10441 gggctggcgc gacagtgatg gagtcaccgg tgtggatgcc catcgcatca aagttttcga
    10501 tagagcagac gatgatgcag ttgtcgtttt tatcacgcac cacttccatc tcgtactctt
    10561 tccagccgat cagcgactca tcaatcagca actctttggt cggagagaga tccagaccgc
    10621 gggcgcaaat ttcttcaaac tcttcacggt tataagcgat accgccgccg ctaccgccca
    10681 tggtaaagga tgggcgaata atgcacggga agcccacgtc agcggcaaca gccagcgctt
    10741 cttccatcgt gtgtgcgata ccggaacgcg cggtttccag accaattttc ttcatcgcta
    10801 cgtcgaaacg acggcggtct tctgctttat caatcgcatc ggcagtggca ccaatcatgg
    10861 tgacaccgaa ctcttccaac acgccctgac gttccagctc cagcgcgcag ttcagcgccg
    10921 tctgaccgcc catggttggc agcaccgcgt ccgggcgctc tttttcaata atcttgcgta
    10981 ccacttccca gtgaatcggc tcgatgtagg tcgcatcggc catttccggg tcggtcatga
    11041 tggtcgccgg gttggagttc accagaatga cgcggtaacc ctcttcgcgc agggctttac
    11101 atgcttgcgc gccagagtag tcaaactcac acgcctgacc gataacaatc gggcccgcac
    11161 ccagaatcag gatacttttt atatctgtac gttttggcat ggctctttta ctcctgatta
    11221 cttagcggtt ttacggtact gctcaattaa cttgataaag tggtcgaaca acggcgcggc
    11281 gtcgtgtgga ccagggctgg cttcagggtg accctggaag ctgaacgccg gcttatcgat
    11341 gcgatgaatg ccctgtaacg taccgtcgaa cagggattta tgcgtgacac gcaggtttgc
    11401 aggtaatgtt gcttcatcca ccgcaaaacc gtggttctgg gcggtgatca tcaccacgtt
    11461 tttctccaca tctttaaccg gatggttgcc gccgtggtga ccaaatttca ttttgacagt
    11521 cttcgcaccg ctcgccagcg ccagcagctg atgaccgaga cagatgccga ataccggaat
    11581 atcggtttcg aggaacttct ggatggcggt aatggcgtaa tcgcacgggg ccgggtcgcc
    11641 aggaccgttg gagaggaaga tgccgtctgg attcattttc agcacatctt ccgcagaagt
    11701 ttgcgccgga acgatggtca ggcgacagcc tctatccacc agcatccgca ggatgttgcg
    11761 cttggcacca aaatcataag ccacgacgtg gaacggcagc tcgtcttctt ttttcgcttc
    11821 tggcaggccg ccggtcaacg tccagctccc ttgtgtccag ctataggctt ctgcggtggt
    11881 cacttctttt gccagatcca tgccgttcag acctgggaac gcgcgggctt tttctaacgc
    11941 cagcgccgca tccgggttat cgcctgcgat aatgcagcca ttctgtgcgc ctttctcgcg
    12001 cagtaaacgc gtcagcttac gggtatcgat atcggcaatc gccacgatgt tatggcgctt
    12061 caggtaagaa gagaggtctt cggtattacg gaagttgctg gcaatcagcg gcaggtcgcg
    12121 aatcaccaga ccttgtgcat gtacctgaga agattcttca tcggcgtcat tagtgccgac
    12181 attgccaata tggggataag taagagtaac gatttgacga gaataggaag gatcagtgag
    12241 gatttcttga taaccggtca ttgaagtatt gaaaacgact tccccaaccg ccgaacctgt
    12301 tgcccctatg gcccgaccgt gaaactgggt tccgtcttcc agaaccaata gcgctgactt
    12361 aatcaaaaca ccctccagag aatactcact cactttattt gcatattaat tcacagtgat
    12421 gacgtaaatc aatgcaaatc tgcttaccga cgaatttctg gcaaacggcg gcattctgga
    12481 gatatgggcg ctaaaagtca acttaatggc aggatttttt atattatttt gtggctcatg
    12541 catgttttac aggcattaag cgacaaaaac atcatctaac ctgttctgga aaacgcttgc
    12601 gcagcaagaa gattgcatga aataaaaacg agcaggaaaa agtggaccaa atggtcaaaa
    12661 tttacattaa aacaacaaaa atagaagacg aaacatgtaa tttttggcag ttatagaaaa
    12721 tttaaaagca taacaccaaa aataaaaggg ctaaaaatta aagcccttta taattaataa
    12781 attaaatgtg ttacttttgc atcataataa acattttgtg gttacaaatt attgagatca
    12841 agcacatctc gcatatcaaa aagaccactt tccttaccac tcaaccacaa agccgatctt
    12901 accgcaccgt tagcaaatgt catgcggctg gacgccttat gggtgatctc cagacgctcg
    12961 ccaatatcgg caaacatcgc ggtatgttcg ccaacgatgt cacctgcacg cacggtagca
    13021 aaaccaatgg tacctggcac acgttcaccg gtatggcctt cacgactgta gaccgcgcaa
    13081 tctttcagat ctttatcaag ggcgtgggcg atcgcctctc ccattgccag tgcggtgcct
    13141 gacggcgcat caactttatg tctatgatgt gcttcaataa tttcgatatc ggtgtagtca
    13201 cccatcactt tggctgcttt ctccagcagc ttaagcatga cgttaacgcc aacgctaaaa
    13261 ttggcggcaa agacaatcgc aatatcggcg gcggcgtcac gaattgcctg tttaccggct
    13321 tcgtcaaacc ccgtagtgcc gatcaccatc cctttgccat gctgccgaca aaaagcgaga
    13381 tggttcagcg taccttccgg acgggtaaaa tcgataaaca catcaaaatc atcttttatc
    13441 gcatcaagac tgctttgcac ggtaacgcct gttttccctg caccagccag ctcaccggcg
    13501 tcgctgccca gtaaagaaga tccttcgcgc tccagcgccg cgcccagctg cacgccttct
    13561 aatgccagtg ccgcctgaat caactggcgg cccatacgcc ccccggctcc cgcgatggca
    13621 acgcggatgt ttgcatcatg catagctatt ctcttttgtt aatttgcata gaccgttttc
    13681 agagtaacca gcgcatcgct acgccgccag ccaaaaacgc tgataattag aaattaatga
    13741 taaacaggga gggatatcag tgaaaggcat gagtcgatga atgactgcat gccgataaca
    13801 tgtgacaggt tacggcacca gagccagcac ctcagccacc cactgttgga agccttttac
    13861 attcagatcc aatgccacct gtacattggc tggtttgccc aggcaaccgt cgatatcaac
    13921 caccgtcgtg cctgaggtaa attcgccttg agtttctacc gcgacaaaac acggcttgag
    13981 ggtgaataag tccgggcgca ccagccaggc gatggcgcag agatcgtgca ttcgcagacc
    14041 gctttgcata ctgccgctac ggtagtggct aaacagggcg tggagcattt ttccggtgcg
    14101 attcaactcc ggtaaagtag cgagatagtc aggggttaat attgcctgat tggtgacatc
    14161 cagaccgcac atgacaattt cgatgccgct acggaagaca caggcagccg cttctggatc
    14221 ggcagcgata ttaaactcgg catttggtgt acagttgccg cgaccagcag aaccgcccat
    14281 gatcaccaga cgacgaatat acggtttaca ttccgggcat tgtaaaagta acagcgcaat
    14341 attggttaac gggccgatgg ccaccagggt aacgggctct ggtgcacgca tcagggcatc
    14401 ccgaatcgcc agaaacgccg gtatcccgag tggctttcgg ttgtgctcaa caaagtcgta
    14461 gccagccatt cccgattcgc cgtgcacaga tgctgcatca cgcggtgcgc gtaccagcgg
    14521 cacagcggct ccctgggcga gcggaatctc cgcattccag aaatgcagca gttgcagggc
    14581 attgcgggta gttttctcaa ccgagacatt acccgcgacg gtggtcatca gttgcaggtc
    14641 gagttcgggg gcaaaaatcg cggcggcaat ggcgacggca tcgtcaatgc cggggtcagt
    14701 atcgaggaag ataggtaaac gcatgttttc ttcataaaaa atgccggtaa caagaccggc
    14761 attttcgcat aacttaggct gctaatgact taatcgactt cacgaatatc gacacgcagc
    14821 tctttcggca cttcgaaaac aatattttct tcacggcctt ccagcggaat ggcttcacca
    14881 ccacccaact gctgcaaacg tgccaccaca ttctgcacca gaatatccgg agccgatgcg
    14941 cccgcagtca cgccgacgca tttaacctct ttcacccact cttcctggat atctttcgca
    15001 tcgtcaatca aaaacgcgtg tttgcccata cgctgggcca gctccgccag acggttggag
    15061 ttggaggagt ttttcgaacc gaccaccaac acaacttccg cctgttctgc cagggcgcgt
    15121 accgcttcct gacggttagt cgtggcgtag cagatgtcat ctttgcgcgg accgacaatt
    15181 ttcgggaagc gtttacgcag cgcgtcgatc acatcagacg tgtcatccac cgacagcgtg
    15241 gtttgggtca taaaggagag cttctcttcg tttttgaccg tcagtttcca cacatcgtct
    15301 ggcgattcga ctagatacat tcccccttcg gggttgctgt actgccccat cgtcccttcc
    15361 acttccgggt gcccggcgtg gccgatgaga atagattctt cgccacggcg actggcgcgg
    15421 gcaacttcca tatgcacttt ggtcaccagc ggacaggtgg catcaaacac cgtcaaatcg
    15481 cggcttttcg cttcgttacg aaccgcctga gaaacaccgt gtgcggagaa aatcaggatc
    15541 gcgccgtccg gcacttcgct aatctgctca ataaagatag ccccacgctc gcgcaagcta
    15601 tcgaccacgt agcggttatg caccacttcg tgacggacat atatcggtgc gccgtaaatg
    15661 gccagcgcgt tttcaacaat gctgatagcg cggtctaccc cggcacaaaa accacgcggg
    15721 ttggccaaca ggatctgcat gttacgcctc cagtgccgga tcgatttcca gcacttcaat
    15781 atcaaaatga acggtctgcc cggccagcgg atggttgaaa tcaacggtaa tggagtcgcc
    15841 gttaatttcg cggatcacac caggcatctc actgccatcc attgcggtaa aaagcatgat
    15901 tgcgccaatt tctggctcgc ctgcatccat aaattcacgg cgggagaagt actgaatcag
    15961 gtccggtgac ggcacgccaa acgccgcatc tggctccagc gagaaggtgg ttttgtcgcc
    16021 cactttcagc cccagcaggt gttgctccag cccttcagaa agagaagcat cgcccaggcg
    16081 gaacagcgcc ggtttaccgt tgttgcgagt agactcggcg gtggtgccat cgtcgagttt
    16141 tagcgtgaag tgcaccagga cggcgctatt gctctgtaca gattcagaca tgcaggtttg
    16201 ctcttatttt tgaggatttt tgccggatgc ggcctacgat ttcgtagcaa tctgtaggcc
    16261 gcatccggca ggatttatta ttgttttttc gctttggaag gtaaaaaccc ttccagcacg
    16321 atcagtgccg caccgacaca aatggcagta tcggcaaggt tgaaggtggc gaagtgccag
    16381 tcgccgacgt agaagtcgat catatcgaca acgaagccgt gccacaggcg gtcgaacagg
    16441 ttgcccagcg cgccgccaat aatcagcgcg taagcgatat tgtttagctt ctgcgtggcc
    16501 ttcgagcgat acatcatcac tgccaggagc acgctaatac caatcgcaat accggcaaag
    16561 aaccaacgct gccagccgcc gctatcggca aggaaactaa acgccgcgcc atagttacgc
    16621 gcataatgca gattaagcga cgggaacagc gggaccgtat cccccagagc aaagttctgg
    16681 aggatcaggt atttgctgcc cagatcgata atcagcacga ctaccaccag ccacagccag
    16741 cgtagccctg ttgaacagat cgattgactc atcaggcaaa cttacgtttt tcaccgtcac
    16801 cggcgacgtt gctgacacag cggccgcaga tttctgcgtg ttccgccacc ttgccgacat
    16861 cctgggtgta gtgccagcag cgtgggcact tctcaccttc ggctttactc aacgcgactt
    16921 tcagcccttt gagtacttcg ctctgctgag catcagcagg tgcgtcgtta tagtctgcaa
    16981 cggtagcgcc ggaggtcaac aggacaaatc gtaattcatc gcccagcgcg gtcagtttcg
    17041 ccgccagttc cggttctgca tacaaggtta ctgccgcttc cagcgagcca cccactttct
    17101 tgtcggcacg cgcttgctca atgactttgt tcacttcgcc acgcactttc aacagctcgt
    17161 cccagaacgc atcgttcatt gcttcactgt ctgccagacc aaacaggcct tcgtaccact
    17221 caccggtgaa gacgtatttt tcacgttcgc ccggcagata gccccacact tcatcagcgg
    17281 tgaaggagag gattggtgcc atccagcgca ccagcgcttc agcgatgtga tacagcgcag
    17341 tctggcagct acgacgcgcc acgctatccg ctttggcggt gtactgacgg tctttgatga
    17401 tgtcgaggta gaaggaaccc atctcaacgg agcagaagcg catcagacgc tgtaccactt
    17461 cgtggaaatc gtatgcttcg tacgccttga ggatgtcttc ctgtgccgct ttcgcacaac
    17521 ctacggccca gcgatccagt accaccatct cttccggttt caccatatct tttgctggat
    17581 caaaaccatt caggtttgcc agcaggaagc gcgcggtgtt acggatacga cgatagctat
    17641 cggcagcacg tttcaggatc tcgtcagaaa cggccatttc accggtgtag tcggttgatg
    17701 ccacccacag acgcagaata tccgcgccca gtttgttcat cacatcctgc ggcgaaacgg
    17761 tgttgccgat ggatttagac atcttgcgac cctgaccatc cacggtaaag ccgtgggtca
    17821 gtacctgacg atacggcgct ttgcctttca tcgcggtgga gatcattagg gaagacatga
    17881 accaaccacg gtgctggtca gaaccttcca gatacatgtc cgctgcgtga ccggcaaatt
    17941 ccggacgcac gtcaacaaca gaagagtggg tagatccgga gtcaaaccat acgtcgagag
    18001 tatccggcac tttcacatac tggtcagctt cgtcgccgag gatctctttc gcatcgagat
    18061 cccaccacgc ctggatgccg tcaacttcaa cgcgtttagc cacttcttcc atcagttcga
    18121 gggtacgcgg atgcagctct tccgtgtctt tgtgcacgaa cagtgacatc ggtacgcccc
    18181 aggtgcgctg acgggagata caccagtcag gacggttagc aaccatcgac tcgatacgcg
    18241 cctggcccca gtccgggagc cactgcacgc ctttgatctc tttcagtgac tgcgcacgca
    18301 gacctttctg atccatgctg acgaaccact gcggtgtcgc gcggaagatg atcggcgttt
    18361 tgtgacgcca gcagcacgga tagctgtgct gcattttctc aacgtgcagc agcgcgcctt
    18421 tttcctgcag cagcgcaacg acgatgtcgt tcgctttgaa gacgttcacg ccatccagcg
    18481 tcggataagt gcccggcaga taagtgccgt ccgggccaac cgggttagcg gtttccaggc
    18541 cgtatttctg accgatcaca tagtcgtccg ggccgtggcc aggcgcggtg tgaacggcac
    18601 cggtaccggc atccagggta acgtgatcgc cgaggattgc cggaacgtcg aagcccataa
    18661 acggatgggt aaagcgcagc agctcaagct ctgcaccttt taccgtgccg agaatggtgt
    18721 aatcggtcac gccgatacgc tgcattacgc tttcaaccag atctttcgcc agaatcacgg
    18781 cctgaccgtc gatctgcacc agcgcatagt cgaaatcagg tgcaatagag attgcgcggt
    18841 tcgcaggcag agtccacggc gtagtggtcc agatcaccag cgagattggg ccgttaacgt
    18901 tgcttacgcc aaatttcgct ttcagcgcat cctgatcgac cgcctggaaa gcgacgtcaa
    18961 tggacggaga agttttgtcg taatactcaa cttccgcttc tgccagtgca gaacggcagt
    19021 ctacgcacca gtgcaccggc ttcgcgcctt tgtgcaggtg accattgccg atgattttgc
    19081 ccagcgcgcg gatgatgttg gcttcagttt tgaagtccat agtcagatac gggtgcgacc
    19141 agtcgcccag cacgcccaga cggataaagt ctttgcgttg accgtcaacc tgggtcgccg
    19201 cgtattcgcg gcacttggcg cggaactcgg cggcggtgaa tttctcaccc ggcttaccgt
    19261 attcttgctc tactttcagc tcgatcggca gaccgtggca gtcccagcca ggcacatacg
    19321 gcgagtcata accggaaagc cctttggact tcacgataat gtctttcaga atcttgttaa
    19381 ccgagtgacc aatatgaatg ctgccattcg cataaggagg gccatcatgc agaatgaagg
    19441 tttttttgcc ttttttagcc gcacggatga tgccgtacag atcatcatca gtccaacgcg
    19501 ccagcattcc cggttcgcgc ttggcgagat cgccacgcat cgggaaccct gtttccggca
    19561 aattcagggt tgatttatag tcactcatca gattctcggt tccgtgtttc ggtttgatta
    19621 cataacaggc ttaagccggt tttgttagcc caaaaaattc gcgggcggtt aattcatcac
    19681 gcgcaatctg cgctttcagt tcgtccagcg acgcaaatcg ctgctcattg cgtatttttt
    19741 tacgcagcac tacttgtata tggcgaccgt aaaggtccat tgcaacatct aacaaatgca
    19801 cttccagctg ctggcgaata ccggcaaccg ttgggcgtgt tccgatgttt gccacgccgg
    19861 gtaacggctt ttcaccgagg cccagcactt ctaccgcata aacccctttc accggggaaa
    19921 cctgacggcg tagcggtaca ttcgccgtcg ggaaacctat agtgcgccct aattcatcac
    19981 cgtggactac acgcccggag atggcaaacg ggtgccccag taaactttct gccagagcca
    20041 gattgtcatc agccagcgcc tgacgcacgg cggtgctgct gatacgcaca ccaccttcgc
    20101 aaaaagtttg cgtactggtg atatcgaagc cgtattccat gccagctttc tgtaataaca
    20161 agaaatcgcc ttcacgacca gcgccaaagc ggaaatcatc acctacggca agaaatttta
    20221 cgcgcaaatg cttcaccaaa agatcgctga tgaaattttg cgcggttaac gccgcaaaac
    20281 gcctgtcgaa acgcacgcac agcacgtaat caacgccaca ctctgcaagg taacgcagtt
    20341 tttcccgcag ccgggtcagt cttgccgggg ctttatcggt agcaaacagt tccagtggtt
    20401 gaggttcaaa aagcatcacc atcaccggta agttgcgctt gcgcccttct tcctgcaagc
    20461 cctgtaacag cgcgcgatga ccgcgatgca cgccgtcgaa attaccaata gtcagcacac
    20521 acccttcttg cggggcctgg ctgagattat gtatgccgcg tatcagcttc atgtctggct
    20581 caaaacagtg aaaatcgtcc gagtatacct tgtacagcgg tcaaggttaa ccggcgattg
    20641 agtaccgaaa tcagaaagaa gtggcttttc attgccatgg agcaaatcac gggaagaaac
    20701 tgaccgcctg ctgcaatttt tatcgcggaa aagctgtatt cacaccccgc aagctggtag
    20761 aatcctgcgc catcactacg taacgagtgc cggcacatta acggcgctta tttgcacaaa
    20821 tccattgaca aaagaaggct aaaagggcat attcctcggc ctttgaattg tccatataga
    20881 acacatttgg gagttggacc ttggctaata tcaaatcagc taagaagcgc gccattcagt
    20941 ctgaaaaggc tcgtaagcac aacgcaagcc gtcgctctat gatgcgtact ttcatcaaga
    21001 aagtatacgc agctatcgaa gctggcgaca aagctgctgc acagaaagca tttaacgaaa
    21061 tgcaaccgat cgtggaccgt caggctgcta aaggtctgat ccacaaaaac aaagctgcac
    21121 gtcataaggc taacctgact gcacagatca acaaactggc ttaatcgcca atttgctgaa
    21181 gctttgtgaa aaagcccgcg caagcgggtt tttttatgcc tgctgctttt gtgatgcgtg
    21241 agagaacaca ctgccggttg ccagatagtc aaaagagaca tgccgttgta ctatctgaac
    21301 tcttcgtggc ttaagaatcg tagaaccatc ggctgataca ttctgaacaa gaatatctac
    21361 tcaatctcca gactccccca caaagaatat ggatattgtg atacacattg aggtattaaa
    21421 cttaagacca ctctattttt gaattaaatt cctatatact ttaaccattc ataaatatct
    21481 cctcattaga cct
//
LOCUS       DAZPEU010000055        21205 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPEU010000055 DAZPEU010000000
VERSION     DAZPEU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.055"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            103..1356
                     /gene="amiC"
                     /locus_tag="ACHU2L_001862"
     CDS             103..1356
                     /gene="amiC"
                     /locus_tag="ACHU2L_001862"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR1356740.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(1431..1507)
                     /locus_tag="ACHU2L_001863"
     tRNA            complement(1431..1507)
                     /locus_tag="ACHU2L_001863"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1471..1473),aa:Met,seq:cat)
     gene            1716..2813
                     /gene="mltA"
                     /locus_tag="ACHU2L_001864"
     CDS             1716..2813
                     /gene="mltA"
                     /locus_tag="ACHU2L_001864"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR1356741.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            3051..3857
                     /gene="tcdA"
                     /locus_tag="ACHU2L_001865"
     CDS             3051..3857
                     /gene="tcdA"
                     /locus_tag="ACHU2L_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR1356742.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3908..4351)
                     /gene="csdE"
                     /locus_tag="ACHU2L_001866"
     CDS             complement(3908..4351)
                     /gene="csdE"
                     /locus_tag="ACHU2L_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR1356743.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(4351..5556)
                     /gene="csdA"
                     /locus_tag="ACHU2L_001867"
     CDS             complement(4351..5556)
                     /gene="csdA"
                     /locus_tag="ACHU2L_001867"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR1356744.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5748..5975
                     /locus_tag="ACHU2L_001868"
     CDS             5748..5975
                     /locus_tag="ACHU2L_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR1356745.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            6326..7243
                     /gene="gcvA"
                     /locus_tag="ACHU2L_001869"
     CDS             6326..7243
                     /gene="gcvA"
                     /locus_tag="ACHU2L_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR1356746.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            7262..7657
                     /locus_tag="ACHU2L_001870"
     CDS             7262..7657
                     /locus_tag="ACHU2L_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR1356747.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7650..8750
                     /gene="rlmM"
                     /locus_tag="ACHU2L_001871"
     CDS             7650..8750
                     /gene="rlmM"
                     /locus_tag="ACHU2L_001871"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR1356748.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8794..9525)
                     /gene="fucR"
                     /locus_tag="ACHU2L_001872"
     CDS             complement(8794..9525)
                     /gene="fucR"
                     /locus_tag="ACHU2L_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR1356749.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(9583..10005)
                     /gene="fucU"
                     /locus_tag="ACHU2L_001873"
     CDS             complement(9583..10005)
                     /gene="fucU"
                     /locus_tag="ACHU2L_001873"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR1356750.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(10007..11426)
                     /gene="fucK"
                     /locus_tag="ACHU2L_001874"
                     /pseudo
     CDS             complement(10007..11426)
                     /gene="fucK"
                     /locus_tag="ACHU2L_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(11535..13310)
                     /gene="fucI"
                     /locus_tag="ACHU2L_001875"
     CDS             complement(11535..13310)
                     /gene="fucI"
                     /locus_tag="ACHU2L_001875"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR1356751.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(13343..14659)
                     /gene="fucP"
                     /locus_tag="ACHU2L_001876"
     CDS             complement(13343..14659)
                     /gene="fucP"
                     /locus_tag="ACHU2L_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR1356752.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAATPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            15206..15853
                     /gene="fucA"
                     /locus_tag="ACHU2L_001877"
     CDS             15206..15853
                     /gene="fucA"
                     /locus_tag="ACHU2L_001877"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR1356753.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            15881..17029
                     /gene="fucO"
                     /locus_tag="ACHU2L_001878"
     CDS             15881..17029
                     /gene="fucO"
                     /locus_tag="ACHU2L_001878"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR1356754.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(17082..17837)
                     /gene="xni"
                     /locus_tag="ACHU2L_001879"
     CDS             complement(17082..17837)
                     /gene="xni"
                     /locus_tag="ACHU2L_001879"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR1356755.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(17948..19315)
                     /gene="sdaB"
                     /locus_tag="ACHU2L_001880"
     CDS             complement(17948..19315)
                     /gene="sdaB"
                     /locus_tag="ACHU2L_001880"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR1356756.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(19373..20662)
                     /gene="sdaC"
                     /locus_tag="ACHU2L_001881"
     CDS             complement(19373..20662)
                     /gene="sdaC"
                     /locus_tag="ACHU2L_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR1356757.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         5242 a         5185 c         5625 g         5153 t
ORIGIN      
        1 accgtttatt agatgacaga ttatgcgtct ttcgctaaag tttccggtca aattagtcgt
       61 ttacttgtta cacagcttag atttcttttg ctcgggagag gcatgtcagg atccaacact
      121 gcaatcagcc gtcgtcgttt actgcaaggc gcgggcgcta tgtggctatt gagcgtaagt
      181 caggtcagcc tggctgcggt tagccaggtc gtggcggtgc gcgtctggcc tgcgtccagc
      241 tacacccgcg tgacggtaga atcaaatcgt cagctgaaat ataagcagtt cgcgttgagt
      301 aaccctgaac gcgtggtggt ggatatcgaa gatgtaaacc tgaactcggt actcaagggg
      361 atggcggcac aaattcgtgc agacgacccg ttcatcaagt cggcgcgcgt cgggcaattt
      421 gacccgcaaa ccgtacgcat ggtttttgaa ttaaagcaaa acgtaaaacc gcagctgttt
      481 gcccttgcgc cggtcgccgg gtttaaagag cgtctggtga tggacctcta tccggccaat
      541 gcacaggata tgcaggaccc gctgctggcg ctgctggagg attacaacaa aggcgacctc
      601 gaaaagcagg tgccgccagc gcaaagtggt ccacaaccgg gtaaagcagg gcgcgatcgt
      661 ccgattgtca ttatgcttga ccctggccac ggtggcgaag actccggtgc ggtggggaaa
      721 tacaaaacgc gcgaaaaaga tgtggtattg caaatagctc gccgtttgcg ctctctgatc
      781 gaggaagagg gcaatatgaa ggtgtacatg acgcgcaatg aagacatctt cattccgttg
      841 caagtgcgcg tagcaaaagc ccagaaacag cgtgctgacc tgtttgtctc tatccatgcc
      901 gacgccttta ccagtcgtca gccgagcggt tcctcggtgt ttgcgctctc aaccaaaggc
      961 gcaaccagta ctgcggcaaa atatctggca caaacccaga acgcctcgga cttgattggt
     1021 ggcgtgagca aaagcggtga ccgctatgtc gaccacacca tgttcgatat ggtgcagtcg
     1081 ctgactattg ctgacagcct taagtttggt aaagcggtac tgaataagct cggaaaaatc
     1141 aacaagctgc ataaaaatca agttgaacag gccgggtttg ccgtactaaa ggcaccagat
     1201 attccctcca ttctggtcga aacggcgttt atcagtaacg ttgaggaaga gcgtaaactg
     1261 aaaacggcga ctttccagca ggaagttgcg gagtctattc ttgcggggat taaagcgtat
     1321 tttgccgatg gggcaacgct ggcgagaagg ggatgataaa aaggcgctga atggcgcttt
     1381 ttttatggga gatcagaaac aaaaaaacac ccgttagggt gttcaataat tggttgcggg
     1441 ggccggattt gaaccgacga ccttcgggtt atgagcccga cgagctacca ggctgctcca
     1501 ccccgcgtcc gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac
     1561 cccgatcacg gatttagcat gattttgaac aaaaacaaac cactaaagcc tgttttttgt
     1621 gcaaaatttg cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc
     1681 gttgcgcctt attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc
     1741 ttcttatggg cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg
     1801 gacagcaata taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg
     1861 ccgttggcgc gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt
     1921 cgtcaccgcg tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc
     1981 gcgcaggcgg tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag
     2041 gtgccgacaa ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc
     2101 gccatacccg ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc
     2161 gtctgccgtc tcgtgcggag atctacgccg gggcattggg cgataaatac atcctcgctt
     2221 acagcaactc tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact
     2281 ttggtgatgg cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc
     2341 gcagcattgg taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc
     2401 aggcgattcg tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac
     2461 agaacccgtc tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg
     2521 cggtgccgct ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta
     2581 ctaccttgct ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg
     2641 aattgcgtct gatggtggcg ttggatgtcg ggggcgcaat caaaggccaa cacttcgata
     2701 tctatcaagg gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac
     2761 gggtttgggt gctgaaaacg gctccgggcg caggtaacgt ctttagcggc tgatgtgata
     2821 ttctgtatgc cgtttttacg gatatgacag gggaggggta acctcacatc ctgattattg
     2881 actaagtgcg cgttgttcat accggatgcg gcgtgaacgc cttatccggc ctacaaatca
     2941 tgcaaatcca aaatattgca ggagttatgt aggcctgata agcgtagcgc atcaggcatt
     3001 tatgcgttta gggtgaggga taacttcgcc ctttttaatt ctgaggtgct atgtctgtgg
     3061 taattagtga tgcatggcgt cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag
     3121 cgttgcaact gtttgctgac gcgcatattt gtgtggttgg tatcggtggt gtcggttcct
     3181 gggcggcgga agcgctggcg cgcacgggga ttggcgcaat cacgcttatc gatatggatg
     3241 atgtgtgcgt caccaatacc aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg
     3301 caaaagcgga agtcatggcg gagcgcattc gccagattaa cccggagtgc cgtgtaacgg
     3361 tggtggatga tttcgtgacg ccggataacg tagcgcaata tatgagtgcc ggttattcgt
     3421 acgtgattga tgccattgat agtgtacggc ccaaagcggc gctgattgct tattgtcggc
     3481 gcaataaaat cccgctggtc acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga
     3541 ttcaggttac cgatctggcg aaaacgattc aggatccgct ggcggcgaag ttgcgcgagc
     3601 gcctgaaaag cgattttggc gtagtgaaaa acagtaaagg taagctcggc gtggattgcg
     3661 tgttttctac tgaagcgctg gtgtacccgc agtcagacgg tacggtgtgt gcgatgaaag
     3721 ccacggcaga agggccgaag cggatggatt gtgcatctgg atttggcgcg gcaacgatgg
     3781 tgaccgccac ctttgggttt gttgcggttt ctcatgcgct gaagaagatg atggcgaaag
     3841 cggcgcgtca gggttaaaca atcaggtcgg atgagacgcg aacgtcttat ccgacccggt
     3901 tctgtgctta aacctgcttc gtcgcagcga taatcgcctc gcttaacgca tttaacccct
     3961 ggctgcgtga ggcgctaagc tgcgcacgta atcccagctc atcaaacaat gccagcggcg
     4021 actgtgcctg taattcggcg gcggttttcc cctcaacggc agtcaacaac accgccaaca
     4081 ggccgcgcac aatgcgccct tcgctgttgc caaagaagtg cattttgccg ttttcagcca
     4141 cggtatatcc cagccagacg cggttttcgc atccggcaat ctctttagcc tgcgctttta
     4201 actcgtctgg caaagccgga agctgtttcc ccagcatgat caactggcga tatttatctt
     4261 cccattgcga caacggtgcg aaggtattgc gtaacgtttc tgcggttacg gttgtgccga
     4321 acggatgtcc ggcgaattgc gggtttgtca ttaatccacc aataattcca gcgcgcggtc
     4381 aacggcattc accagcgcat ccacatcact ctttgtatta tatggcgcaa atgaggcgcg
     4441 cagtgtgccg gttacgccta attctgccag tagcggctga gcgcaatgtt gcccggcccg
     4501 caaggcaata ccgtactccg ccagtagcgt caccatatcg ctgtgatgaa cgccagcaaa
     4561 atcaaaggcc agcaggctgg aatcctggca gcggaatgaa cgaaagcctg gacgtttcgc
     4621 cagcgcttct tctgcaagcg ttgctaagct acggctccag ctttcggcct ggttgatatc
     4681 gtaatctgcc agccattcca gcgccgcgct taatcctatg acgccagcga catttggcgt
     4741 tcctgcttcc agtttccacg gcgcagattg agtggtgaaa ccgtcaaaac tcacttcgtg
     4801 aatcattttg ccgccaccca gccagggcga catcgcttcc agcagttctg atttgccata
     4861 cagcacgccg atacccgtcg ggccatacag tttgtgacca gagaaagcat agaaatcgat
     4921 atccagttgc tgaacatccg cggggaaatg caccgtcccc tgagcaccat caaccatcac
     4981 caccatcccg gctgaatgag caaaggtaat cgctcgcgcc agatccgggc aaccgccagt
     5041 cacgttcgac atctgaccca acgccagaat ccgactacgg ggagtaatca gctctggcaa
     5101 caaatcgaca tccggcagtc gctgcgcatt aagcggcaat ttcaccactt tggctctagt
     5161 ttgttgggcg accatcagcc aggggacgag gttagcgtgg tgttctgcca cgctgacaat
     5221 aatctcatcg cccggttgca gacgcggacg cgcatagcat tgtgccacca tgttgatgga
     5281 ttcagtggtg ccgcgcgtcc agacgatagt tttatcatcc ggtgcattca gtaattgcgc
     5341 cactttctct cgtgcagctt cataacgcgc ggtcaggcgt tgggcttcgg caaactggct
     5401 gcgatggacg tttccggcgc tcaggctgta aaactgtcga gtggcttcaa ccacggcttc
     5461 aggtttaagc gcggtcgcgg cgctgtcgag atagacgccc gcatcctgta gtgcgggaaa
     5521 ctgggcgcga aactgcgcgg gattaaaaac gttcatggta ctcctcggct tgatgcaccg
     5581 atcgtgacgc aaaattcact ttgatacaag gagagtgcta accatcagaa ttatctggat
     5641 atcctggcga agccgcgtaa gtaacatatg ctgaatagtg ttaggcgtta attttagcct
     5701 gttaatcaat actattaaga tttattaatc gttttaagga gaagatgatg aaaaagactg
     5761 ccgcaattat ttctgcctgt atgctgactt ttgccctgag cgcctgttcc ggttcgaact
     5821 atgtgatgca caccaatgac ggacgtacca tcgtctctga cggcaaacca cagactgata
     5881 acgatatcgg tatgatttcg tataaagacg ctaatggcaa caaacagcag atcaaccgta
     5941 ctgacgtgaa agagatggtc gaactggaac agtaattcgc gatcgcaagg taaaaaaaag
     6001 caccgcaatt aggcggtgct acattaatca ctatggacag acagggtaaa tgtacaggaa
     6061 gtgaaaaaag gtagctttgc taccatggtc tgaatcgcag accaattgca aacacaacaa
     6121 cacaacatca caaccgtaag ccaaaagttc accagaatac gcattccgat aaaacttttc
     6181 gttccggctc aggaagtgcc gccactatag gtatttgctg gtagaagctc aacggacaat
     6241 ttataatggc tcagattaaa aaaactaata ggttacacag tgtgatctaa ttgttaaatt
     6301 catttaacat caaagtttaa aagccatgtc taaacgatta ccaccgctaa atgccttacg
     6361 agtttttgat gccgcagcac gccatttaag ttttactcgc gcagcagaag agctttttgt
     6421 gacccaggcc gcagtaagtc atcaaatcaa gtctcttgag gattttttgg ggctaaaact
     6481 gttccgccgc cgtaatcgtt cactcctgct gaccgaggaa gggcaaagct atttcctcga
     6541 tatcaaagag atattttcgc aattaaccga agcgacgcgt aaactccagg cccgtagcgc
     6601 caagggggcg ttgacggtca gtttactccc cagtttcgcc attcattggt tggttccgcg
     6661 actttccagc tttaattcag cttatccggg aattgacgtt cgaatccagg cggttgatcg
     6721 tcaggaagat aagctggcgg atgatgttga tgtggcgata ttttatggtc ggggcaactg
     6781 gccggggcta cgggtggaaa aactgtacgc cgaatattta ttgccggtgt gttcgccgct
     6841 actgctgaca ggcgaaaaac ccttgaagac cccggaagat ctggctaaac atacgttatt
     6901 acatgatgcg tcacgccgtg actggcagac atatacccga cagttggggt ttaatcatat
     6961 caacgttcag caagggccaa tttttagtca tagcgccatg gtgctgcaag cggctattca
     7021 cgggcaggga gtggcgctgg caaataacgt gatggcgcaa tctgaaatcg aggccggacg
     7081 tcttgtttgc ccgtttaatg atgttctggt cagtaaaaat gctttttatc tggtttgtca
     7141 tgacagccag gcagaactgg gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc
     7201 cgctgctgaa caagaaaaat tccgctttcg ttatgaacaa taatttacgt agggtacgac
     7261 catgaccagc cgttttatgc tgattttcgc cgccattagc ggcttcattt ttgtggcact
     7321 gggcgctttt ggtgcgcatg tgttaagtaa aaccatgggg gccgttgaga tgggctggat
     7381 ccaaaccggt ctcgaatacc aggcgtttca tacgctggcg atcttaggtc tggcggtggc
     7441 aatgcagcgt cgcatcagta tctggtttta ctggagtagc gttttcctcg cgttaggcac
     7501 ggtgctgttc agcggcagcc tttattgcct ggcgctgtcc catctgcgtt tgtgggcgtt
     7561 tgtcacaccg gttggcggcg tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc
     7621 tatccgttta aagcgcaagg gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg
     7681 gctttgaaaa agagtgcgcc gcagaaatta ccgataaagc cggccagcgg gaaattttcg
     7741 gttttgcccg cgtgaaagag aatgcgggtt atgtcattta tgaatgttat caacctgatg
     7801 atggcgataa gttaatccgt gagctgccgt tcagttcatt aatttttgcc cgccagtggt
     7861 ttgtggtggg ggaactcctg cagcatttgc cgccagaaga tcgtattacc cccattgtcg
     7921 gcatgttaca gggtgtagta gagaagggcg gtgaactgcg tgttgaagtt gccgatacca
     7981 acgaaagcaa agagttgctg aaattctgcc gtaagttcac tgttccgcta cgcgctgcct
     8041 tgcgcgatgc tggggtgctg gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat
     8101 tctttattgc accaggatgc tgctataccg gttactcata cagcaacaat aattcgccgt
     8161 tctatatggg cattccgcgc ctgaaatttc cggcagaggc gccgagtcgt tccacgctca
     8221 aactggaaga ggcatttcat gtgtttattc ctgcggatga gtgggatgaa cgcctggcga
     8281 acgggatgtg ggcggtggat ttaggcgctt gccctggcgg ctggacctac caactggtga
     8341 agcgcaacat gtgggtttat tccgtcgaca acggcccgat ggcgcaaagt ctgatggata
     8401 ccgggcaggt gacgtggctg cgggaagacg gtttcaaatt ccgtccgaca cgcagcaata
     8461 tctcctggat ggtatgcgat atggttgaaa aaccggctaa agttgcggca ttgatggcgc
     8521 agtggctggt taatggctgg tgccgtgaaa ccattttcaa tctcaaactg ccgatgaaaa
     8581 aacgctacga agaagtatca cacaatctgg cgtatattca ggcacagctt gatgaacatg
     8641 gcataaatgc tcagattcag gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc
     8701 acgtccgccg catctgggct gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc
     8761 cggggcgtcg tctccggcct gctacccttt ccgtcaggct gttaccaaag aagttgcaac
     8821 ctggcgctca tcagaaataa tgtgcgttac ctcatccaga tgcccgatgc gggcttcccc
     8881 tgaacgatta aatttacttt tatcaatcaa taacaacgat tgcgcggcac gttttaatag
     8941 catcgatttg taatcagcgt tgatcgcatt ggagtcccac agtgcgccgc tgctatcgat
     9001 cccttcacaa gaaaaaataa acagatcgat ttccagcgat ttaagttggg aaatcagcga
     9061 gggattgacg taacagccat atttgcgctc aagtgtgccg ccggaactga tcagttgaat
     9121 gcgttcgcgt ttaccgagtt catggcaaat cggatggcta ttggtgaaga cctgaatgtt
     9181 gatgtcaggc aactggcgtg ccagatacca gcaagttgaa ctggcgtcta aggctatcac
     9241 catcccttct tcaatccacg cgagcgcctc gcgcgcgata tctgctttat gcgcataatg
     9301 gcttttcagc ctgatgtgaa agggatcgcc actgtcttga ttttgacggt ggatatattt
     9361 agcgcgtcca tgattgcgca gaattttacc ctgcgtctgt aattcattga gatcgcgacg
     9421 aatggtttct ttactgacct ttagctgttc agagagagct tccgtggtca ggctggtatg
     9481 gttcagcagc aggtcgacta tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc
     9541 attcgataac cgcccgcgtc atgcgggcgt accggtatga gattacggtg ttaccccttt
     9601 ttttaaaaga atattcccgt acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt
     9661 ttgcgcccgt tcataaaacg caaaacgatt gatgcggatg atgtcaggac acggggcttg
     9721 tagtgaaagc gcattacggt aacgtcgttc tacttcagga tcgagagtgt caccctctac
     9781 cgccgccatc atcaccagcg gcggggcata actgtccagc tcaaataacg ggataatcgc
     9841 ctggagcaag tcgctcacca acaggccatc agcgcggatc acctgcggtt ccatcgaatg
     9901 ggcgggaaag tgagcatcgg aaaaaataat ttcatctcca tgtcccatct ctgccagcac
     9961 tttcaatagt tcgggagaaa ttaacggcga aattgttttc agcatttcac acttcctcta
    10021 taaattcagg ttcagtttgc gggtagaaat aacggtactg ataatgaatc tgtgcacggg
    10081 cttcttccgg gctgttaaat tcccctacgc cataccaacc gaacagcgca gctcctgcga
    10141 cggtcgtttc ggcatcgtcg agaactttta ccggaatatc aagcatattg gctttaatct
    10201 gattccacaa tgtgttgcga cttcctccac cgactaacaa taattcatag gctttaaagt
    10261 gcccgatttt ttccagcatc tgtagattgc gctgtaattg cgcagttaac ccttccagcg
    10321 ccgcgcgata gaaatgcccc cgcgtggtat taagcgtcac tccttgccag ccagcgttct
    10381 gacacgacaa taaatcacac tgcattttca caccatccgc gccaggtgcg atcagacgag
    10441 cttcttcaat caacatttgc cagggtgttt cagccgtcca gaacagtttt ctcacccatt
    10501 ccagcacgcc ggatgccagc cattgcatac ctgggttata caaccatgcc tggctatcta
    10561 gttcgcaggt ggaaccggcg tactgactta acagcgaagt atcaacctag gcgctgcgaa
    10621 ccattaaaat ttcccatgta ccggaggaga gcacgggttc attttgttcg gcaccagcgc
    10681 caaaaagggc gaactgggta tcgtgacctg cggaaatcac cggtatgcca acgggtaagc
    10741 cgagcattgc tgcggcgttg ttctgtagcg taccaatctg ttcacccgct tccaccagac
    10801 gagggaagag tcggcgtgga ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt
    10861 gctggatatc cagcatctgg ctggttccgg ccatcgtgat atcagtagtg aattcgctgg
    10921 ttaaacggtg gttaatcagc gacgaaatga agagccaggc gtgcgcgcgt tccagcagtt
    10981 gtggatgatt ttctttcaac cacaccaact tatataacgt attgaaacta aaggctccga
    11041 cgccagaaat agcctgcaac cgctgtgcgg agattaaccg ttcaatattg tccataaccg
    11101 ctgctgttcg cggacatttc cagctaatga tcggatagag cagattgcct tgcttatcta
    11161 ccagagcgcc atccacacta aaggtggtga cggcgatacc gcggatgtgg cattcagtca
    11221 gttcactatt gatttgccga cagcaatcag caaagcgttg caaaatggca gtctaaagac
    11281 cactggtgcc aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg
    11341 gcaacaattt ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg
    11401 agtaccagga taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgtt
    11461 tatccggcta accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat
    11521 cagggtgtaa tgtattaacg cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc
    11581 tggccttcaa tatccatgcc gtgcgcagcc caggcagaag gacgatacac tttggtctct
    11641 tcaacgttgt gcatacatac cgggatacgc agcatggagg caagggtgat aaagtctgcg
    11701 ccaacgtggc cgatggtcag aactccgtgg ttggcacccc agttcgccat taccgaatac
    11761 acatccgtaa acggcccttt accagtgagg cgcggtgcaa accaggtggt tggccaggtt
    11821 gagttggtgc gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct
    11881 tccgcgattt gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac
    11941 gggacgccgc cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg
    12001 atcgccgggc accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc
    12061 cagtgcggct tcatcgtcgg gttaccttcg ctgtcgcgtt gtttacagga accgtccagc
    12121 gcagcagaac ctgagttgat caaatggatg atgccgtgtt ctgccagtcc gtccagttta
    12181 tgccccgtta cacgctcaat tgcttctggt gaccagtagg tacgcacatc ggcaaatacc
    12241 tgagcggtgc cggtgagctg gtgacccatt agcattgcca cgccgttaag actgtcgttt
    12301 tcggtcgcca cgacaaaggg ttcgcgcacg ccattccagt caaatgaact gttgaggatc
    12361 gcttcggcgg tgtcaccatt gggatattga tcggtccagt gacgttgccc ctggaagccc
    12421 gcagcgatgg cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg
    12481 ttgccttgca tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg
    12541 cggctttgct cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag
    12601 tttttatcag cccaggccag tgccatttcc agttcggctt cgtcgtaaat cttctgatcg
    12661 atacggcgac gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca
    12721 aagaagttgt gatcaacaat agaaccggcg atacccatcg aaacgccgcc cagcgacaga
    12781 tagcttttac ctttcatgct ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt
    12841 tcttcaacat cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg
    12901 gagaatgccg ggatgccttt ctggctgtga gctgccagag ccgcagccag gtaaacagcg
    12961 ccggggcgtt cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatgtcg
    13021 atggtttcac tgccatagca ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg
    13081 ctgctgaatt tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa
    13141 atgacacact cgacggcagc tccgcaggca tggcgcagtt tctcggtcag cagtgcggcc
    13201 gtagctttcg ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga
    13261 ccgtcaataa ccgggcggat accaattttc ggtaagctga tttttttcat tacagattcc
    13321 tcactttatt cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga
    13381 taaagatgac cgcgaagcag agtgcgggga tcagttcagc agtggggatg ttgcccgccg
    13441 cgtcactgac aaaacccatg accggagtga caataccgcc gccaataatg gtcataacga
    13501 tgaaggacga accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg
    13561 ttgggtactg aatcgacata aaggcgctgc ataaagtcag ggctattaag cccacatgac
    13621 cgccagcgaa ggctgagatc aggcacagtg ccatagcgat taatgcgtag gcggccagga
    13681 ctttgtgtgg tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca ataaagaagc
    13741 acaccatggt tccggttaaa tagttagcgg caaagcctgc agtcatacct agaatttctt
    13801 ctacagcgta gcgaatcaaa tagctccagc aggccgtttg tgcgccgaca tagcagaatt
    13861 gcgctaatac cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc gatgcggaga
    13921 acgatccttg tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca
    13981 tgatcagcag ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg
    14041 ataataccag gctgtgttta tacgcactca attgctctgg agacatttta tcgagaacgt
    14101 cttgcgattg atgtggcacg ttagacaaaa taagactttg cccaaagaca accgcgataa
    14161 ttgcgccaaa cgagttaaat gtttgcgcaa gatttaagcg gaagtgacta ctgctttccg
    14221 gccctaatac cgtaacaaaa ggggttgcgg cagtttccag acaacctaat ccggctgcaa
    14281 taataaatag gccaactaaa aataaggtgt agttcattat ttctgcagcg ggccagaata
    14341 atgcagcgcc caaggcatat aaaaataatc cggtaataat ccctgcttta taactgagtt
    14401 ttttcatcaa tatcccagca gggattggga taatgaaata accaaagtaa aaggccgatt
    14461 ggatcaggcc agcctggaaa tttgtcagcg taaaagcctg ctggaattga ggtaataaaa
    14521 tgtcgttaag gttattggct accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg
    14581 gaataatgta acttctgctt tgccctgcat ctttatctac cgcacggtaa ctctgcgttt
    14641 gtattgatgt gtttcccata gcatcctctt aggttcagaa gcttaagcac gtaatcggtt
    14701 atgttttcaa aagtgcaaaa ccagcgagtt cgggcttcat atcggtgaat atttgcctgt
    14761 ctgaaatgaa ctcatggatt cattttcaaa taaaaaacgc tctgtaatat gacggcggtc
    14821 acacttatta gctggaaggt gactttatgt gactaccatc actttaataa tcgaaatttc
    14881 atgcccgttt cagaaaataa atcataaaaa acacaaacca gatgaccgta ttaattgttt
    14941 gtgccgaaaa cgggcatttc tggcgagggg aaatggaaaa atgaccgttt tgataaatat
    15001 ttttaagagc ggtcattttt aacggggtta taaaaagaga acttgcagtt atttactgtg
    15061 attagttgag ctaggtcaca aaatataagc ttgtgaattt ctttcttgtt aatcaggaag
    15121 aagatggtgg gtaattaaac ggctaattca atagtgtgaa aggaacaaca ttattgccct
    15181 gttttgaatc agagagaggt agctaatgga acgaaataaa cttgctcgtc agattattga
    15241 cacttgcctg gaaatgaccc gcctgggact gaaccagggg acagcgggga acgtcagtgt
    15301 acgttatcag gatgggatgc tgattacgcc tacaggcatt ccatatgaaa aactgacgga
    15361 gtcgcatatt gtctttattg atggcaacgg taaacatgag gaaggaaagc tcccctcaag
    15421 cgaatggcgt ttccatatgg cagcctatca aagcagaccg gatgccaacg cggttgttca
    15481 caatcatgcc gttcattgca cggcagtttc cattcttaac cgatcgatcc ccgctattca
    15541 ctacatgatt gcggcggctg gcggtaattc tattccttgc gcgccttatg cgacctttgg
    15601 aacacgcgaa ctttctgaac atgttgcgct ggctctcaaa aatcgtaagg caactttgtt
    15661 acaacatcat gggcttatcg cttgtgaggt gaatctggaa aaagcgttat ggctggcgca
    15721 tgaagttgaa gtgctggcgc aactttacct gacgaccctg gcgattacgg acccggtgcc
    15781 agtgctgagc gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct atgggttacg
    15841 gattgaagag taatttcgta aagcaacaag gagaaggatg atggctaaca gaatgattct
    15901 gaacgaaacg gcatggtttg gtcggggtgc tgttggggct ttaaccgatg aggtgaaacg
    15961 ccgtggttat cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt
    16021 ggcgaaagtg accgataaga tggatgctgc agggctggca tgggcgattt acgacggcgt
    16081 agtgcccaac ccaacaatta ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg
    16141 cgcggattac ctgatcgcta ttggtggtgg ttctccacag gatacttgta aagcaattgg
    16201 cattatcagc aacaacccgg agtttgccga tgtgcgtagc ctggaagggc tttccccgac
    16261 caataaaccc agtgtaccga ttctggcaat ccccaccaca gcaggtactg cggcagaagt
    16321 gaccattaac tacgtgatca ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc
    16381 gcatgatatc ccgcaggtgg cgtttattga tgctgacatg atggatggta tgcctccggc
    16441 gctgaaagcc gcgacgggtg ttgatgcgct cactcatgct attgaggggt atattacccg
    16501 tggcgcgtgg gcactaaccg atgcactgca cattaaagcg attgaaatca ttgctggggc
    16561 gctgcgagga tcggttgctg gtgataagga tgccggagaa gaaatggcgc tcgggcagta
    16621 tgttgcgggt atgggcttct cgaatgttgg gttagggttg gtgcatggta tggcgcatcc
    16681 actgggcgcg ttttataaca ctccacacgg tgttgcgaac gccatcctgt taccgcatgt
    16741 catgcgctat aacgctgact ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg
    16801 cgtgaaagtg gaaggtatga gcctggaagg ggcgcgtaat gccgctgttg aagcggtgtt
    16861 tgccctcaac cgtgatgtcg gtattccgcc acatttgcgt gatgttggtg tacgcaagga
    16921 agacatttcg gcactggctc aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg
    16981 tgaagcaacg cttgaggata ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg
    17041 tgataatgcc ggatacgatt tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat
    17101 tgctgtaaat tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga
    17161 aacgccatct ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga
    17221 ttctcatata tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt
    17281 ccgattcccg caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg
    17341 ggcaactgct gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt
    17401 ttctggaagt aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct
    17461 gtcgaaacaa tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag tgtggcggct
    17521 aagtcatcgg cttcgttgcc gctggctgac cagcacggga cgccgcgttg ctcaaaggcg
    17581 gcgcgtaatg caggcatctc gtcgtgcaac tcttccggca ttggcggtcg atccgctttg
    17641 taatctggta aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg
    17701 accgcgtggg ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg
    17761 acacagggcg aaccctgaac ggcatgaatg cgacggataa gattcagtgc atcgacaata
    17821 agcaaatgaa cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg
    17881 ccggaacagg ctatggctgg ttgagaaaac aggaagaggc ttcgcaaaac gaggcctctg
    17941 ggagagatta atcgcaggca acgatcttca tcgccaggcc gccgcgagag gtttcgcggt
    18001 acttggcgtt catgtcttta cctgtttcgt acatggtttc gataacttta tcgaggcaga
    18061 cgcgcggttc gctggtacgg cgcagtgcca tacgcgcggc gttcaccgct ttcactgctg
    18121 caatggcgtt acgctcaatg cacggtacct gaacctgccc cgcgaccggg tcacaagtca
    18181 gaccgaggtt gtgctccatc gcgatttccg ctgcgatgca aacctgcgcc gggcttgcgc
    18241 ctaacagttc tgccagacca gctgccgcca tagagcacgc cacgccaact tcaccctggc
    18301 aacccacttc ggcaccagaa atcgacgcgt tcatcttata aagagaacca atggcgctgg
    18361 ctaccagcag gtaacgagcc agtgagttag cgttcacttc gcggataaac ttatcgtagt
    18421 acgccagcac agccgggata atcccgcacg caccgttagt cggcgcagtc accacgcgac
    18481 cgccagcagc gttctcttcg ttcactgcca gtgcaaacat gttgatccag tcaacaaccg
    18541 ccatcgggtc agtggtggtt ttatcctggc tgaccagcat ccggcgtagt gccgcagcac
    18601 ggcgtggaac gcgcagtttg ccaggcaaca cgccttcggt ggaaataccg cgctcaatac
    18661 cgccacgcat gacttcccag acgttcgcca ggtgctgttc cagctcttct ttactgtgca
    18721 gcgccagctc gtttttcatc atcaggccag agagtgacag cccggtttcc tgacaatgtt
    18781 tttgcagatc ggctgctgaa ctgtacggat aaggaacttc aaccggtgcg ctatcctgct
    18841 ggccaaaatg ctcttcatca acgataaagc caccgccaat agagtagtaa gtctggctgt
    18901 aaacgacttt atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt
    18961 tgtctgcatg gaagttcatg cactgatcaa ccgggaactc cacttcatgc tgaccgtttg
    19021 ccagcatcag gcgaccatga gtattcacat cctgaataaa accggggatg gaatcgatat
    19081 ccacggtatc cggcaggtta cccgccaggc ccataataat ggcgatatct gtgtggtggc
    19141 ctttaccagt cagagagagc gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa
    19201 gcaggttacg ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg
    19261 tatgagaact ggaagggcca atgccgattt tgaaaatatc gaatacgcta atcataggaa
    19321 atacatcgcg ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga
    19381 acagagagta gaagattgcg gagattgcaa tcagacccat cacgacaacg aatacgttgc
    19441 tgatgtgacc gctgtactta cgcattgccg gtactttctg aattgcgtac atcggcatca
    19501 ggaacaggat catcgcgatg attgggccgc ccagggtttc aatcataccc aggatgctcg
    19561 ggttcagggt ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg atacggttca
    19621 gcttgttgat ttcgatagac ttacctttac cacgcagaga tttaatcacc ataccgttga
    19681 agccttcacg tgcgcccagg tagtggccga ggaaggattt ggtgatagcg ataatcgcga
    19741 taatcggagc catccacgcg ataaccggtg cgttaaagtg gttagccagg taagacagaa
    19801 tcgagatgtt ctgctcttta gccgcagcca ggtctgccgg agtcaggctc agtacgcagc
    19861 tgaagacgaa gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg
    19921 agcatttctg ttctgccata tcgccgtact cttcacgctt cgcaacggcg aaagaagaga
    19981 tgatcggaga gtggttgaac gagaacacca ttaccggaat tgccagccac agggtcatcc
    20041 acagaccgtt tccggttgca gatgcagtgt ccagagacag cgtttccagt gctgcgccgt
    20101 tccactgcgg gatcaggtac agagccagca gcatcagtac gccaacaaac gggaatacca
    20161 gaatactcat cgctttaacg atcatctgct caccaaagcg aacgatggtc atcataccca
    20221 cgatcaggat cagcgacaga atcgcacgcg gcggtggcgt catacccagc tggtgagaca
    20281 tgaagctttc aacggtattg gtgattgcca cgctataaac cagcaggatc gggtagatag
    20341 cgaagaagta gagcagggta atcagtttac ctgcgccaat accaaagtgt tcttctacaa
    20401 cctcggtgat gtcttcgccc gggtttttac cagacagtac gaagcgagtc aggccgcggt
    20461 gagcaaaaaa cgtcatcggg aacgcaagga tagccatgat gatcagcggg atcataccgc
    20521 caacaccggc gttgattggc aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc
    20581 ccagcatcca catggtgtct gtcttgcgcc aggcactacg agagtctttc gacgcaatcg
    20641 tgctggtttg agtcgtttcc atctatttct cctggaggaa tgcaaataat tcagctttta
    20701 gccagttcag cccgggaagg tgcctgaccg taaaaatgaa atctgtctat cggcgatggc
    20761 ttaatcattt accgccgtaa taatttgagg gcggaaagat acacacaagt tatgtatcta
    20821 tcagtgatct tgatcccaaa tacaataatc tgcttggaag atagattatc ccaacgcttt
    20881 actctgtaaa tcaaaagtaa aagagaattt aagggcaata tttgcccatt aaacgacatc
    20941 tgacttcttc cggggcgcgc aatatatgta ggttgaactg aaaaaatcaa tacttttagt
    21001 taatcatggc gataatttct ttttcatagc gataaaatac ggtttatgaa aatagggaaa
    21061 cgtttgtgtt gcaacataaa ttgtatgaca tattaactgt gatgatattt gtcgcgtgag
    21121 ggtggtaaga aacattcata taacaaatga aatgagttga tgagtaagtg accaaaaacg
    21181 agaaaacccg gcaacaaacg tcacc
//
LOCUS       DAZPEU010000056        21084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPEU010000056 DAZPEU010000000
VERSION     DAZPEU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.056"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(2..298)
                     /locus_tag="ACHU2L_001882"
     CDS             complement(2..298)
                     /locus_tag="ACHU2L_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR1356758.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="ACHU2L_001883"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="ACHU2L_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR1356759.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHU2L_001884"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHU2L_001884"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR1356760.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="ACHU2L_001885"
     CDS             complement(1784..2323)
                     /locus_tag="ACHU2L_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR1356761.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="ACHU2L_001886"
     CDS             complement(2353..3096)
                     /locus_tag="ACHU2L_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR1356762.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="ACHU2L_001887"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="ACHU2L_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="ACHU2L_001888"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="ACHU2L_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR1356763.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="ACHU2L_001889"
     CDS             complement(4151..4657)
                     /locus_tag="ACHU2L_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR1356764.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="ACHU2L_001890"
     CDS             complement(4703..5203)
                     /locus_tag="ACHU2L_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR1356765.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="ACHU2L_001891"
     CDS             complement(5289..5468)
                     /locus_tag="ACHU2L_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR1356766.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="ACHU2L_001892"
     CDS             complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="ACHU2L_001892"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR1356767.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="ACHU2L_001893"
     CDS             complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="ACHU2L_001893"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR1356768.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="ACHU2L_001894"
     CDS             complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="ACHU2L_001894"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR1356769.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="ACHU2L_001895"
     CDS             complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="ACHU2L_001895"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR1356770.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="ACHU2L_001896"
     CDS             complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="ACHU2L_001896"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR1356771.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12400..12493)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="ACHU2L_001897"
     CDS             complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="ACHU2L_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR1356772.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12652..13533
                     /gene="rnm"
                     /locus_tag="ACHU2L_001898"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             12652..13533
                     /gene="rnm"
                     /locus_tag="ACHU2L_001898"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR1356773.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13530..14150
                     /locus_tag="ACHU2L_001899"
     CDS             13530..14150
                     /locus_tag="ACHU2L_001899"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR1356774.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14251..15123
                     /gene="rluB"
                     /locus_tag="ACHU2L_001900"
     CDS             14251..15123
                     /gene="rluB"
                     /locus_tag="ACHU2L_001900"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR1356775.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="ACHU2L_001901"
     CDS             complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="ACHU2L_001901"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR1356776.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15750..16508)
                     /locus_tag="ACHU2L_001902"
     CDS             complement(15750..16508)
                     /locus_tag="ACHU2L_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR1356777.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16728..17777
                     /gene="sohB"
                     /locus_tag="ACHU2L_001903"
     CDS             16728..17777
                     /gene="sohB"
                     /locus_tag="ACHU2L_001903"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR1356778.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17813..18064)
                     /locus_tag="ACHU2L_001904"
     CDS             complement(17813..18064)
                     /locus_tag="ACHU2L_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR1356779.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18444..21041
                     /gene="topA"
                     /locus_tag="ACHU2L_001905"
     CDS             18444..21041
                     /gene="topA"
                     /locus_tag="ACHU2L_001905"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR1356780.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         5293 a         5239 c         5441 g         5111 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg atatattaac aattacctga ggaatatgtg
     5641 acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat
     5701 attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag cgaaacggta
     5761 aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg
     5821 ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac
     5881 aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt
     5941 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
     6001 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
     6061 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
     6121 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
     6181 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
     6241 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
     6301 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
     6361 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
     6421 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
     6481 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
     6541 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
     6601 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
     6661 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
     6721 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
     6781 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
     6841 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
     6901 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     6961 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     7021 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     7081 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     7141 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     7201 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     7261 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     7321 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     7381 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     7441 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     7501 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     7561 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     7621 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     7681 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     7741 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     7801 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     7861 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     7921 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     7981 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     8041 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     8101 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     8161 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     8221 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     8281 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     8341 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     8401 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     8461 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     8521 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     8581 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     8641 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     8701 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     8761 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     8821 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     8881 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     8941 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     9001 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     9061 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     9121 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     9181 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     9241 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     9301 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     9361 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     9421 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     9481 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     9541 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     9601 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     9661 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     9721 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     9781 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     9841 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     9901 tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac
     9961 tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga
    10021 acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc gggtgctcac
    10081 cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca
    10141 ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt
    10201 tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg
    10261 tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc
    10321 cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga
    10381 tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg
    10441 ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt
    10501 gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc
    10561 cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac
    10621 aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg
    10681 tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat
    10741 gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca
    10801 gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag
    10861 tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac
    10921 tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg agcggatcac
    10981 aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc
    11041 gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct
    11101 taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag
    11161 ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa ctttggtgag
    11221 atcggcgatg tagcggctgc caggggtgca aatgcgtgcc agatcattac gggcgagatc
    11281 aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca gttcaatacg
    11341 gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc
    11401 aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc tttccggcga
    11461 cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact
    11521 ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga
    11581 tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc
    11641 accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg aaaccactgg
    11701 cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg
    11761 ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg
    11821 atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc agttattttc
    11881 cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga acaggccgcc
    11941 gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca ataaacggaa
    12001 agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg
    12061 ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg caggcagggc
    12121 gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt
    12181 gtcacctaat gctgtaatgc gcagcgcact gtctatcagc agcaggcttt ttaaatcatc
    12241 tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc cacacaactg
    12301 gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca gttcgagagt
    12361 cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag
    12421 gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc
    12481 gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt
    12541 gcgtactagt taactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca
    12601 gacccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac
    12661 acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg
    12721 acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc
    12781 gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg
    12841 gcgctgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat
    12901 attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag
    12961 acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata
    13021 cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat
    13081 tttgcgcgtt tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag
    13141 tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct
    13201 attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat
    13261 cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg
    13321 atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg
    13381 cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc
    13441 gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa
    13501 cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata
    13561 acccgcagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg
    13621 tctatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg
    13681 agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg
    13741 atctttctga actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa
    13801 acaacacgcc gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc
    13861 tgttgcagga gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg
    13921 cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg
    13981 aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga
    14041 ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata
    14101 cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact
    14161 ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg
    14221 cctgtgaagg cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc
    14281 cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    14341 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt
    14401 cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    14461 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg
    14521 gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac
    14581 gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
    14641 catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac
    14701 gcgaaactgc gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa
    14761 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
    14821 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
    14881 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
    14941 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc
    15001 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt
    15061 gcggtgaaac gtcacagtca ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt
    15121 taatcaaagt atgccggacg tcatatccgg catttttaca gattaataat caatccctat
    15181 ttgcgcctta acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc
    15241 cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg
    15301 tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat
    15361 atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc
    15421 atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt
    15481 aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc
    15541 attaggccag gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc
    15601 gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat
    15661 gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg
    15721 ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt
    15781 tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag
    15841 agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt
    15901 gcgcgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga
    15961 tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca
    16021 ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg
    16081 tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg
    16141 atctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg
    16201 agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt
    16261 gccagttgtt ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc
    16321 tgacgcccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg
    16381 cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc
    16441 ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg
    16501 taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt
    16561 tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa
    16621 aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc
    16681 tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt
    16741 ctgaatatgg tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg
    16801 ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca
    16861 acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact
    16921 cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag
    16981 cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg
    17041 tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa
    17101 cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg
    17161 gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa
    17221 acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct
    17281 gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg
    17341 cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca
    17401 ccgccgggca gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg
    17461 agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta
    17521 tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg
    17581 cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga
    17641 tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat
    17701 tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc
    17761 aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa
    17821 ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg
    17881 tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca
    17941 cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac
    18001 gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt
    18061 tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat
    18121 ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag
    18181 ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg
    18241 cttgacattc gaccaaaaat ccgtcgtgct atagcgcctg taggccaaga cctgttaact
    18301 cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga
    18361 gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa
    18421 gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa
    18481 aagccaaaac gatcaacaag tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc
    18541 acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca
    18601 ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg
    18661 ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg
    18721 tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg
    18781 accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc
    18841 gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca
    18901 aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc
    18961 gcgtggtggg gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg
    19021 ccggtcgtgt gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag
    19081 cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag
    19141 cgttggagtt gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac
    19201 aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag
    19261 acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg
    19321 ccagcacccg tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg
    19381 aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa
    19441 atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga
    19501 atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg
    19561 tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt
    19621 taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc
    19681 tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg
    19741 gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag
    19801 tcgataaagg cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc
    19861 cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc
    19921 gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag
    19981 aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa
    20041 atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg
    20101 tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc
    20161 agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg
    20221 ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta
    20281 ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca
    20341 ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa
    20401 ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc
    20461 tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg
    20521 agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa
    20581 aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca
    20641 acgaagagtg taaaaacaca cgtaagattt tacgtaacgg tgaagtggca ccaccgaaag
    20701 aagatccggt gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc
    20761 gtgacggtgc tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc
    20821 gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt
    20881 atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc
    20941 gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag
    21001 cattttatgt tgatggcaaa tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt
    21061 tttataaaca aagggtcgcg aaag
//
LOCUS       DAZPEU010000057        20780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPEU010000057 DAZPEU010000000
VERSION     DAZPEU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.057"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..579
                     /locus_tag="ACHU2L_001906"
     CDS             <1..579
                     /locus_tag="ACHU2L_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1356781.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHU2L_001907"
     CDS             597..1235
                     /locus_tag="ACHU2L_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1356782.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHU2L_001908"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHU2L_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR1356783.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHU2L_001909"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHU2L_001909"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR1356784.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHU2L_001910"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHU2L_001910"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR1356785.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHU2L_001911"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHU2L_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR1356786.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHU2L_001912"
     CDS             complement(5552..6613)
                     /locus_tag="ACHU2L_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR1356787.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHU2L_001913"
     CDS             complement(6610..8007)
                     /locus_tag="ACHU2L_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR1356788.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHU2L_001914"
     CDS             8163..9161
                     /locus_tag="ACHU2L_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1356789.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHU2L_001915"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHU2L_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACHU2L_001916"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACHU2L_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHU2L_001917"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHU2L_001917"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR1356790.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHU2L_001918"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHU2L_001918"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR1356791.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="ACHU2L_001919"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="ACHU2L_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(15296..16071)
                     /locus_tag="ACHU2L_001920"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="ACHU2L_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(16089..17141)
                     /locus_tag="ACHU2L_001921"
     CDS             complement(16089..17141)
                     /locus_tag="ACHU2L_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR1356792.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="ACHU2L_001922"
     CDS             complement(17172..18014)
                     /locus_tag="ACHU2L_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1356793.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="ACHU2L_001923"
     CDS             complement(18001..18882)
                     /locus_tag="ACHU2L_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1356794.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="ACHU2L_001924"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="ACHU2L_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHU2L_001925"
     CDS             complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHU2L_001925"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR1356795.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5071 a         5669 c         5052 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttgccctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttacta
//
LOCUS       DAZPEU010000058        20708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPEU010000058 DAZPEU010000000
VERSION     DAZPEU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.058"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHU2L_001926"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHU2L_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR1356796.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHU2L_001927"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHU2L_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR1356797.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHU2L_001928"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHU2L_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR1356798.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHU2L_001929"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHU2L_001929"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR1356799.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACHU2L_001930"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACHU2L_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR1356800.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACHU2L_001931"
     CDS             complement(8217..9110)
                     /locus_tag="ACHU2L_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR1356801.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHU2L_001932"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHU2L_001932"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR1356802.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHU2L_001933"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHU2L_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR1356803.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHU2L_001934"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHU2L_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR1356804.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHU2L_001935"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHU2L_001935"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR1356805.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHU2L_001936"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHU2L_001936"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR1356806.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACHU2L_001937"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACHU2L_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHU2L_001938"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHU2L_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR1356807.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHU2L_001939"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHU2L_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR1356808.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHU2L_001940"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHU2L_001940"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR1356809.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHU2L_001941"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHU2L_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR1356810.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5726 c         5029 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaat
//
LOCUS       DAZPEU010000059        20581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPEU010000059 DAZPEU010000000
VERSION     DAZPEU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.059"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(190..1839)
                     /gene="actP"
                     /locus_tag="ACHU2L_001942"
     CDS             complement(190..1839)
                     /gene="actP"
                     /locus_tag="ACHU2L_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR1356811.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1836..2150)
                     /locus_tag="ACHU2L_001943"
     CDS             complement(1836..2150)
                     /locus_tag="ACHU2L_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR1356812.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2350..4308)
                     /gene="acs"
                     /locus_tag="ACHU2L_001944"
                     /pseudo
     CDS             complement(2350..4308)
                     /gene="acs"
                     /locus_tag="ACHU2L_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4701..6137
                     /gene="nrfA"
                     /locus_tag="ACHU2L_001945"
     CDS             4701..6137
                     /gene="nrfA"
                     /locus_tag="ACHU2L_001945"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR1356813.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6182..6748
                     /gene="nrfB"
                     /locus_tag="ACHU2L_001946"
     CDS             6182..6748
                     /gene="nrfB"
                     /locus_tag="ACHU2L_001946"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR1356814.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6745..7416
                     /gene="nrfC"
                     /locus_tag="ACHU2L_001947"
     CDS             6745..7416
                     /gene="nrfC"
                     /locus_tag="ACHU2L_001947"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR1356815.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7413..8369
                     /gene="nrfD"
                     /locus_tag="ACHU2L_001948"
     CDS             7413..8369
                     /gene="nrfD"
                     /locus_tag="ACHU2L_001948"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR1356816.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8389..10107
                     /locus_tag="ACHU2L_001949"
     CDS             8389..10107
                     /locus_tag="ACHU2L_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR1356817.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQENSLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10100..10483
                     /gene="nrfF"
                     /locus_tag="ACHU2L_001950"
     CDS             10100..10483
                     /gene="nrfF"
                     /locus_tag="ACHU2L_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR1356818.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10480..11076
                     /gene="nrfG"
                     /locus_tag="ACHU2L_001951"
     CDS             10480..11076
                     /gene="nrfG"
                     /locus_tag="ACHU2L_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR1356819.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11418..12731
                     /gene="gltP"
                     /locus_tag="ACHU2L_001952"
     CDS             11418..12731
                     /gene="gltP"
                     /locus_tag="ACHU2L_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR1356820.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12809..13498)
                     /gene="yjcO"
                     /locus_tag="ACHU2L_001953"
     CDS             complement(12809..13498)
                     /gene="yjcO"
                     /locus_tag="ACHU2L_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR1356821.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13592..15739)
                     /gene="fdhF"
                     /locus_tag="ACHU2L_001954"
                     /pseudo
     CDS             complement(13592..15739)
                     /gene="fdhF"
                     /locus_tag="ACHU2L_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(15937..17403)
                     /gene="mdtP"
                     /locus_tag="ACHU2L_001955"
     CDS             complement(15937..17403)
                     /gene="mdtP"
                     /locus_tag="ACHU2L_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR1356822.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17400..19442)
                     /gene="mdtO"
                     /locus_tag="ACHU2L_001956"
     CDS             complement(17400..19442)
                     /gene="mdtO"
                     /locus_tag="ACHU2L_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR1356823.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19442..20473)
                     /gene="mdtN"
                     /locus_tag="ACHU2L_001957"
     CDS             complement(19442..20473)
                     /gene="mdtN"
                     /locus_tag="ACHU2L_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR1356824.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20492..>20581)
                     /locus_tag="ACHU2L_001958"
     CDS             complement(20492..>20581)
                     /locus_tag="ACHU2L_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356825.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4612 a         5447 c         5641 g         4881 t
ORIGIN      
        1 attatagcgc ataaaatatc cttataatta acaagaaaga aaaggcatat tctctgcatt
       61 atcattttct acgactgtca aaaatcgctc attttttaat gagtttattt gtttaatatt
      121 atgggaaaag gtgatgcatg ttggagagga agcgtattcc ccggtcaggc gaccggggaa
      181 ggggagagat taatgtgcgc ggccttgctc aacgccaaag ccggtctggg agcggataaa
      241 ctgcgcgcgg aacagttcac gctcgcgcgc cccttccgct gagttatcgg ttgccgagaa
      301 gaaccagatg ccgaggaatg ccacgctgat agagaacagc gccgggtatt catacgggaa
      361 gatggctttt tcgtgaccga ggatctgtac ccaaatagtc gggccgagga tcatcagcac
      421 cactgcggta atcagcccca gccagccgcc cagcatcgcg ccacgggtag tcagtttcga
      481 ccagtacatg gaaagcagaa tgatcgggaa gttacagctc gccgcgatgg caaacgcaag
      541 gcctaccata aaggcgatgt tctgattctc aaacagcacg ccgaggataa tcgcaatcac
      601 gccgaggatc agtacggtga tttttgatac ccgcagctct tcacgttcgg tcgcgccttt
      661 tttgaagacg ttagcgtaca agtcatgcga aaccgccgat gcgcccgcca gcgtcagatc
      721 cgcaaccacc gcgaggatag tggcgaaagc aaccgctgaa ataaaaccga ggaataggtt
      781 gccgcccact gcattcgcca ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc
      841 cgccgcgtct ttatattccg gattcgcacc aacaagcatg atcgcgccga agccgataat
      901 aaaggtcaga atatagaagt agcccataaa tccggtggcg tagaacacgc tcttacgtgc
      961 ttcgcgggca tcgctgactg taaagaagcg catcagaatg tgcggcaagc ccgccgtacc
     1021 aaacatcagt cccagcccca gagagagcgc ggagatcgga tctttcacca gtccgcccgg
     1081 tttcatgatg tcgacacctt tcgggtgtac cgccatcgct tcgctgaaca gattgttgaa
     1141 gctaaagccg acgtgtttca tcaccataaa ggccataaag ctggcaccga acagcaacag
     1201 cacagctttg ataatttgta cccaggtggt cgccagcatg ccgccgaaca ggacgtacat
     1261 catcatcagc acgccgacca gcaccaccgc aatgtgatag ttaaggccaa acagcagctc
     1321 gatcagttta cctgcgccta ccatctgagc gataaggtaa agcgccacca ctaccagaga
     1381 accacaggcc gaaagaatac gaatcggccc ttgtttcaga cgataagagg ccacatcggc
     1441 agaggtgtag cgtcccaggt tacgcagacg ttcggcaatc agaaacagaa tgattggcca
     1501 gcccaccagg aagcccaacg agtagatcag cccgtcatag ccggaggtaa acaccagcgc
     1561 ggagatcccc aagaatgagg cggcggacat atagtccccg gcaatcgcca gcccgttctg
     1621 gaagccagtg atattgccgc ctgcggtgta gtagtcgcta cgagaacgta cgcgttttga
     1681 tgcccagtag gtaatgccga gcgtaaacac gacgaaaatc aggaacataa taatcgcctg
     1741 ccagttcgtt ggctggcgct ctacggcccc gctaatagca tccgcggcgt tagctgcgaa
     1801 agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg
     1861 acttcattat taagacggtc gaattcgccg ttcgcccgcc agatgtagat accggtgaga
     1921 acaaaggaga tcacaatcac tccaacacca attggaatac cgcgtgtgac gctggtgttc
     1981 ggattcagcg gcgtgcccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     2041 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2101 tccctgaaat gcgcattgtc ttctatccgc tgataaatcg taccattcat cacagattct
     2161 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2221 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2281 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgatt cgcatccggc
     2341 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgaccac
     2401 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2461 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2521 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2581 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acataggcgt agatcgcctg
     2641 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2701 cagcgccgac tctatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2761 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2821 atacatattt ttgaaggtgg aaaaataggt ctgctcaaaa cgttcgtgat cgccaaacag
     2881 cgtacgcgcc tgaccaggcc aggagtcggt gatcaccagg ctaccttcgg tagccccttc
     2941 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     3001 cgaaccggct ttcagctcgg tagcgccagg cagcggggtg atcatgaaac cgccggtttc
     3061 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3121 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgta gcgacgaacg
     3181 gtcggtgcct tcaatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3241 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3301 ccagttcggt acgccttcaa acatcagcgt ggttgcgccg caggccagcg ggccgtacag
     3361 caaataactg tgtccggtaa cccagcccac atcggcggtg caccagtaga tatcacccgg
     3421 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3481 gtgcagtacg ccttttggtt ttccggtaga accggaggta tagagaataa acagcggatc
     3541 ttcggcgttc atctcttccg cctagtgctg atcgctggct tgctcaacca ggtcgtgcca
     3601 ccacaggtcg cgcccttcct gccagtcgat gtttccgcca gtacgcttca gtaccaccac
     3661 atgctctacg ctggtgacgt tcgggttttt tagtgcgtca tcaacgtttt tcttcagcgg
     3721 aatactacgc ccggcgcgca cgccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3781 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcatcgc
     3841 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3901 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3961 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     4021 gatgatggcg gtgcgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4081 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtatt
     4141 tttcaccttc tggtacggtt tgatccagtc gagaattttt ccctgttcgc cccagaaggt
     4201 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4261 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4321 cttgtaggat gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4381 tttagtattt gggcgacaga tcacgcaaaa gtagaatggt gcaaataaac ggcagggtaa
     4441 tttttgaagg tcaccaacaa aagttgatta attctttgag gaacatgcag ttatgcatgc
     4501 tgttgaaaaa gaggaagata ctgactaact ctaaagtggt atttcacatg cacttacaat
     4561 tgattaaaga caacattcac agtgtggtta tttgttacac ataggggtga gcaatgtcat
     4621 gacagtgtag gtgcggttac tgtcgtgaaa aacaataaaa accgccattg caacaatggc
     4681 gcaattcgga tgaagcccct atgacaagga taaaaataaa cgcacgccgt atcttcagct
     4741 tattgattcc ttttttcttt ttcacttctg ttcacgctga acaaacggca gctcccgcaa
     4801 aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc gcagcatcac gatcaatatc
     4861 tctcctggaa agccacctcg gaacagtcag agcgtgttga cgccctggcg gaagatccac
     4921 ggctggtgat cctgtgggcg gggtatccct tctcgcgcga ttacaacaag cctcgtggac
     4981 atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg
     5041 aagatggtcc gctaccgatg gcgtgctgga gttgtaaaag cccggatgtg gcgcgtctga
     5101 tccagaaaga cggcgaagat ggctacttcc acggtaaatg ggcgcgcggc ggcccggaaa
     5161 tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc ctcaccagag ttcgccaaag
     5221 gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa gccattggta
     5281 aaccttttga gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg
     5341 tggagtatta cttcgacggc aaaaacaaag cggttaaatt cccgtgggat gacggcatga
     5401 aagtcgaaaa tatggagcag tattacgaca aaattgcctt ctctgactgg actaactccc
     5461 tgtcgaaaac gccaatgctg aaagcgcagc acccggaata tgaaacctgg acagcgggca
     5521 ttcacggtaa aaacaacgtg acctgtatcg actgccatat gccaaaagtg cagaacgccg
     5581 aaggcaaact ctacaccgac cataaaattg gtaatccgtt tgataacttc gcccagactt
     5641 gtgcgaactg ccatacccag gataaagctg ccttgcaaaa agtggtcgcg gaacgtaagc
     5701 aatcgattaa cgacctgaaa atcaaggttg aagatcaact ggttcacgct cacttcgaag
     5761 cgaaagcggc gctggatgca ggcgcgacgg aagccgaaat gaagccgatt caggacgata
     5821 tccgtcatgc ccagtggcgt tgggatctgg cgattgcttc ccacggtatt catatgcacg
     5881 caccggaaga aggtctacgg atgctcggta cggcgatgga taaagcggcg gatgcgcgca
     5941 ccaaactggc gcgcctgctg gcgaccaaag gtatcaccca tgaaatccag atcccggata
     6001 tctcgaccaa agagaaagcc cagcaggcca ttggcctgaa catggaacaa atcaaggccg
     6061 agaagcagga cttcatcaaa acggtgatcc cgcagtggga agagcaggca cgtaaaaacg
     6121 gtctgttaag ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa
     6181 tatgagcgta ttacgttcgt tattaactgc tggggtgctg gcgtcgggcc tgttgtggag
     6241 cctgaacgga attaccgcta cccctgcggt gcaggcatct ggtgatcgct acgaagttac
     6301 ccagcagcgt aacccggatg ccgcctgtct ggactgtcat aaaccagata ccgaaggtat
     6361 gcatggcaaa catgcttccg ttatcaaccc gaataacaaa ttgccggtca cctgcaccaa
     6421 ctgtcacggc caagcatcac cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa
     6481 cgagccgatg tataaggtcg gagagcagaa cagcgtctgt atgtcctgtc atctgccgga
     6541 acagttgcaa aaagcgttct ggccgcacga tgtccacgta accaaagtgg cgtgcgccag
     6601 ctgccattcc ctgcatccac agcaagatac gatgcagacg ttaagtgaca aaggacggat
     6661 taagatttgc gtcgattgcc acagcgatca gcgcaccaat ccgaacttta acccggcgtc
     6721 tgttccgttg cttaaggagc agccatgacc tggtctcgtc gccagtttct caccggcgtc
     6781 ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc
     6841 aatggggtgc gttacggcat ggtgcatgac gagtcgttat gcatcggctg taccgcctgt
     6901 atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt
     6961 cgtagcgagc cgcagggcga atttcctgat gtgaaatatc gcttcttccg taagtcttgc
     7021 cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc ttttcgtgat
     7081 gccgctagcg ggattgtcga tgttaatcct gatctctgcg tcggttgtca gtactgcatc
     7141 gccgcctgtc cgtaccgcgt gcgctttatc catccggtca cgaagacggc ggacaaatgc
     7201 gatttctgtc gtaagaccaa tttgcaggcc ggtaagcttc ccgcgtgcgt tgaagcttgc
     7261 ccgacgaagg cgctgacgtt tggcaatctg gacgatccta acagtgagat ttcgcaactg
     7321 ctgcgccaga agcctactta ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac
     7381 cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca gacttccgca tttcattttg
     7441 aatcgctggt gtgggactgg ccgattgcca tctacctgtt tttgattggt atttctgccg
     7501 gtctggtgac gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca
     7561 gtacgttgct gcgcaccacg ctgattgtcg ggccgggcgc ggtgatcctc ggtctgttga
     7621 tcctcgtctt ccacctgaca agaccgtgga ccttctggaa gctgatgttc cactacagtt
     7681 ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg gtgctggtgc
     7741 tgtggctggc gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc tggttgccga
     7801 agctggggat cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa
     7861 cgctgatgct ggtgttggcg gtgctgctgg gggcttatac cggctttctg ctgtcggcgc
     7921 tgaaatcgta tccgttcctc aataacccga gcctgccggt gctgctcctc ttctccggca
     7981 tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc agtaacccgc
     8041 attccacgga agcgcacttt gttcaccgga tggaaatccc cgtggtatgg ggtgaaatct
     8101 tcctgctggt ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa gttcgtgcgc
     8161 tggtggcggc attaggtggc ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc
     8221 tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc ggcattcctg
     8281 ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc
     8341 tctacgccgg acagttgacg gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc
     8401 cttcctgaag tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg
     8461 ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact cacttgcatc
     8521 ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac gtattgtttt
     8581 ctcatcagcg atttctcggt catttatgtc gcccaacata gctacagcct gctgtcgtgg
     8641 gaactcaaac tggcggcggt gtggggcggt catgaaggtt cgctgctgct ttgggtgctg
     8701 ctgctttccg cctggagcgc gctgtttgcc tggcattatc ggcagcaaag cgatccgcta
     8761 tttccgctga cgctagccgt tttatctctc atgctcgccg cactgctact gtttgtggtg
     8821 ctgtggtccg atcccttcgt gcggatattt ccaccagcaa tcgaaggccg cgatctcaat
     8881 ccgatgctgc aacatcccgg tcttatcttt catccaccgc tgctttacct tggctatggc
     8941 ggtttgatgg tagcggcgag cgtggcgctg gcgagtctac tgcgcggcga gtttgatgcg
     9001 gccagcgccc gaatttgctg gcgctgggcg ttacctggct ggagtgcatt aacggcgggg
     9061 atcatcctcg gttcctggtg ggcctactgc gaactcggct ggggcggctg gtggttctgg
     9121 gatccggtgg aaaacgcctc tttattaccc tggctttctg ccactgcgct gctgcacagt
     9181 ttgtccctga cacgccagcg ggggattttc cgccactggt cgctgttgct ggcgatagtt
     9241 actctgatgc tgtcgctgct gggcacctta attgtccgtt ctggcattct ggtttcggtt
     9301 catgcgttcg cactggataa cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt
     9361 agccttgcgt ctttggctct gtatggctgg cgagcgcggg acggtggccc ggcggtgcgt
     9421 ttttcggggt tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg
     9481 ctactgatcg tgctggtggg aacgctttat ccgatgattt acggcctgct gggctgggga
     9541 cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt taccgtttgg tctgttgatg
     9601 ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac gcgtgcagct tccggcgctg
     9661 gtagctcatg ctggcgtgct gttatttgcc gcggggatcg tggtttccag tgtcagtcgt
     9721 caggagaaca gcctgaattt acagccgggt cagcaggtga cgctggcagg atacaccttc
     9781 cgttttgagc gactcgatct gcaagccaga ggcaattaca ccagcgaaaa agcgatagtg
     9841 gcgctgtttg accatcagca acgcattggt gaattaacgc cggagcggcg tttttatgaa
     9901 gcacgccgtc agcaaatgat ggaaccgtca attcgctgga acggcatcca tgactggtat
     9961 gcggtcatgg gtgaaaaaac cagagcggat cgttacgctt ttcgtttgta tgtacaaagc
    10021 ggtgtgcgct ggatctgggg gggaggattg ttgatgattg cgggcgcatt gttaagcgga
    10081 tggcggggga agaagcgcga tgaataaagg gcttctcacg ctactgctgt tatttacctg
    10141 ttttgcgcgc gctcaggttg tagacacctg gcaattcgcc aatccgcaac aacagcaaca
    10201 ggcgttaaat attgccagcc agttacgttg tccgcagtgc cagaatcaaa acttactcga
    10261 atccaatgcg ccggtggcgg tcagtatgcg ccatcaggtt tatagcatgg tgtcggaggg
    10321 gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc tacggagatt ttgttcgcta
    10381 taacccaccg ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg tgttgttatt
    10441 gctgatggca ctgatcctct ggcgagtgag ggctaagcga tgaaacagcc tcaaataccg
    10501 gtgaaaatgc tgacaacact cacgatattg atggtattcc tctgtgtcgg cagttatctg
    10561 ttaagcccaa aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga tccgctacat
    10621 cagtttgcca gccagcaaac cccggaagcg cagcttcagg cattgcagga taaaatccgc
    10681 gctaatccac aaaacagcga acaatgggcg ttactgggcg agtactatct gtggcaaaac
    10741 gattacagca attcgctgct ggcgtatcgt caggcgttgc aactacgtgg tgagaacgct
    10801 gaactgtatg cggcgctggc gacggtgctt tattaccagg ccagccagca tatgaccgcc
    10861 cagactcgcg caatgatcga caaagccctc gcgctggact ctaacgaaat caccgctctg
    10921 atgctgctgg cttccgatgc gtttatgcag gcgaattacg cgcaagccat cgaactatgg
    10981 caaaaagtga tggatctcaa ctcaccccgg atcaaccgga cacagctggt cgagtcgatt
    11041 aatatggcaa aactgttgca gcggagatcg gattaatcat ctggtgatga agattatgtc
    11101 attgagctgc ataaaaaata atcgaatgaa catatgccaa aaataatcac caatcagtat
    11161 tattgcagat taaacaaata aaaatctttc cataacaaat ggttattcat taatcctgcc
    11221 ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca
    11281 acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca
    11341 caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca
    11401 ttgaggaagt tattctgatg aaaaatataa aattcagcct ggcctggcag attctgtttg
    11461 ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact
    11521 ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga
    11581 ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca
    11641 aacagctcgg gcgtattggc gcgaaaacca ttatttactt cgaggtgatc accaccgtcg
    11701 ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt
    11761 cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca
    11821 gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatt gtggcgtcga
    11881 tggcgaaagg cgaaatgctg ccgatcatct ttttctcggt gctgtttggt ctggggcttt
    11941 cttccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc atctctgaaa
    12001 ccatgtttaa agtgactcac atggtgatgc gttatgcacc ggtgggtgtg tttgcgctga
    12061 ttgcggtgac ggtggctaac tttggtttct cgtctctgtg gccactggcg aaactggtgc
    12121 tgctggtgca tttcgccatt ctgttcttcg cgctggtagt gctgggaatt gtggcacgtc
    12181 tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt
    12241 actccactgc cagctctgaa agcgtactgc cgcgaattat tgagaagatg gaagcctacg
    12301 gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt aaccttgatg
    12361 gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc
    12421 tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg
    12481 ctggcgtggc tggcgtgtcg tttgtggtgt tgctggcaac gctgggtagc gtaggtattc
    12541 cgctggaagg tctggcgttt attgctggtg ttgaccgtat cctcgacatg gcgcgtactg
    12601 cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat
    12661 ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt gataaaactg
    12721 cggatcaata attgaagatt gccggggata tcccccggca atgtgtggat gcctgatgcg
    12781 acgcttgccg cgtcttatca ggcctacctt aaccgttggt taatttttca aactcttcac
    12841 acccggtatc aaacccttcc atacagctca ggttcaacca gtgcagcgcc ttttgtttgt
    12901 tcttctcgat aaaaccttcc tcaccgttta agaacatcat tcccgcccag tactcggaat
    12961 aaccggttcg ggaaattgca gagctgcgtt tgaaatacca ggttgccttg tcatcgtcgg
    13021 ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag catttgggca tcgacggcgg
    13081 agtcattctc cagatcttcc gaggcgtttt ctaacagcga aatcgccttt ggataatccg
    13141 gtttacccgc ttgagtattt accagaatat gcgccagcgt tacttcacct tctttactgc
    13201 ccgcttgcgc cgctttctct gcaagaactt ttgcttgtgg atagtccaga ctgaccggat
    13261 tggtgatttt gatctgcgcc aacagcgcgc aggcatcagc atcaccgctg tcggcggctt
    13321 tctgtgccca atattcggct ttgctcaaat cgccggagct aaaccagctg tcggcaagaa
    13381 aatattgtgc acgtcggtcc ccggcctctg ctgcctttaa atactggctg ccaggctcgg
    13441 agtcgttcgc gtgggcaaag aatgtcaaaa acaacatcaa tgcgataatt tttttcattt
    13501 tgataatacg attgggtagg gagtaaccag tataaagagt tcgaatggat aaaaaaacag
    13561 cctccgaaag gaggctgtag aaaggactgt attacgccag tgccgcttcg cgcaggcgag
    13621 ttttcaactt gttgtactcg tcaatcacgt actgctcggc ggcgcgctga tcggagatcg
    13681 gctcgacgcg cacggcgcag tatttgtatt ccggcgtttt cgtaatcggg cttaagcttt
    13741 cggtaaccag ctcgttacag gcaccaatcc accactggta ggtcatgtaa atcgcccctt
    13801 tgttcggacg atcgctgacc tgcgcacggg tgataatttt gcctttacgc gagtgcactc
    13861 aaaccaatgc ctcatcttca atacccagac gtttggcgtc ttcggtattg atttgtgcgt
    13921 agccaggttc atcagccagc gccgccagtg ccgcacagtt accggtcatc gaacggcaag
    13981 agtagtgacc aacttcacgc accgttgaca gtaccatcgg gtactcgtcg gtgagtttgt
    14041 cgattggcgc tacccagtcg caggtgaaga actgcgccag accgttcggg gtatcaaact
    14101 tctctttaaa cagataagaa gtcccctgat cggcatctga agtatcgcgg caaggccact
    14161 gaatgaagcc cagttcgccc attttctcgt aagtcgcacc gtagaaatcc gggcacagat
    14221 gacgcaactc atcccagatc tcctgggtgt tgttgtagtg catcggataa cccatacggg
    14281 tggcgatttc actgatgatt tgccagtccg ttttcagatc ccatttcggt tcaactgctt
    14341 tgaagaaacg ctggaagcca cggtcagccg cagtaaacac gccttcatgc tcgccccacg
    14401 acgttgacgg caaaatgaca tccgccgccg acgcggtttt ggttataaag atgtcctgaa
    14461 cgatgaccag ttccagatct tcaaaggctt tacgtactgc cgacagctcc gcatcagttt
    14521 gcagcggatc ttcgcccata atgtacgcgg cacgcacttc gccatgcgct gcgcggtgcg
    14581 gcagctcgct gatgcgatag ccggtatgag caggcaggct ttccacgccc caggctttgg
    14641 cgaatttttc gcggttagcc ggatctttca cgtactggta tcccggatac gtatccggca
    14701 gcgcgcccat atcgcaggca ccctgcacgt tgttctgacc acgaaccggg tttacacccg
    14761 catgcggctt accgaggtta ctggtcagca tcgcgaggct ggtcagagaa cgcacggttt
    14821 ccacgccctg gtagaactgg gttacaccca tgccccacag gatggcggcg cttttcgcct
    14881 gggcatacat ccgtgccgcc tgacgaatct cactggcgct gacaccggtg atatcttcaa
    14941 ccgactccgg cgtgtagcct tcaacgattt tacgatactc ttcaaagcct tctgtacggg
    15001 aagcgacgaa cgctttgtcg tacagatttt cttcaataat gacatggccc atcgcattca
    15061 acagcgcgat gttcgagccg tttttcagtg caatgtgcat gtcagcaatg cgcgcggttt
    15121 caattttgcg cggatcgcag acgataattt tcgccccgtt acgtttagcg ttaattacgt
    15181 gattcgccac gattgggtgg gaatccgccg ggttgtaccc gaaaacgaac actaaatcgg
    15241 tattatcaat ttcgttaata gcattgctca ttgcgccatt accgaccgat tggtgcagac
    15301 ctgcaaccga tgggccgtgt cagacacgag cgcagcagtc aacgttattg gtaccaataa
    15361 cggcgcgcgc aaatttttgc attacatagt tggtttcgtt acccgtacca cgcgaggagc
    15421 cggtcgtctg gatggcatcc ggaccgtact tctctttgat ggcgctcagg cgctcggcaa
    15481 cgtaattcag tgcctcatct caggaaacag gttcgagttt gccgccacgc tggcgacgga
    15541 tcatgggggt tttcaggcgc ggggtcagga tctgggtatc gttaatgaaa tcccagccat
    15601 aataaccctt cagacacagg gtaccctggt tggttttccc ctgcgctgcc tccgcccgga
    15661 cgattttgcc gttatcgacg accaggttga ttttgcaacc tgatgcgcaa taggggcaaa
    15721 ccgtgacgac ttttttcatc ggtctcgctc cagttaatca aatcacgcat acgcgctctc
    15781 gactacagta tgcatctttt atgccacatt ttatttgggg tcattccctg atattacggg
    15841 cactatttat tcaaaactct gacgaaaaac aggctgtcgt cagttttgac gtgacgaaac
    15901 gaaataccgc gtgacagcca tcacgcggca gacattttat tttttctcga cgacgggagc
    15961 cgcctgatac ccgccgccca gcgatttcat caactgaatg ctttggatca cccggcggct
    16021 gtccagcatc agtaatgaca tctcttcggc aagtactggc aaccgggctt cagtggcctg
    16081 taagcggctg gttaagccgc gctgataggc ggcctcggca gcgcgctggg tgaagcgcgt
    16141 tgcttccacg tgttcagcct gcatttctcg ctcgtcgttg agcgtttgca gacgcgtgcc
    16201 gttgacggca acgtcacgca ccgcgttcag tactgactgg ttgtaacgtt caatcatcat
    16261 gttgctggcg gcgcgcgtgc cttcgagatt ggcattcaac cgtccaccgt caaacagcgg
    16321 caatttcaga cccgggatga agttgaactg gcgactggtt tttttgaata aggtatccag
    16381 gtggatggca tcaaggccga aaaacgcttt gatatcaaag ctcggataga acaacgcccg
    16441 cgcggaatcc acctgatcta atgacgcctg aacataccag cgcatggctt gcagatccgg
    16501 gcgtctggcg agcaactcat aagagagtgt cgccggaatg ccggtctgga ctcgtggtaa
    16561 tgccacaggt ttgatctccg gcatatcgct ggctcccgcg ccaatcaatg cgcgcagaga
    16621 ttctctcgtt tcggtgattt gccctttgac ggcagcaatt tgtttatcga ccgccagaat
    16681 ctgtgcccgc gcgccgtgga aaggcacttg tgcttccaga ccgtgcgcca ctttactttg
    16741 gtgcgctttc accgcgtaat caatcacatc gcgagtttgt tctaacagat cgagcatctg
    16801 atagctggcc tgcatactgt aataaagctg cgctacgccc gtggtcagcg atagctctac
    16861 tgctgcggtt tctgccagcg cggcattatg cgcgccaatg gcggcggcaa ccgctgagcg
    16921 atgcacaccc cacaaatcaa gatccagtcc ggcaaacaaa cctactgtgg cttccgtata
    16981 gtacggcccg tccatcccca gtgcgggcgc atccatcgca taagggctta aaaagccgtt
    17041 cgccgagaca cgttggcggt taagcatccc taacgctgcc acctgcaact gtgaaccggc
    17101 atctaacaaa tcggcctgcg attgcgcttt ttcttcccgc agtttcgctt cggcgagggt
    17161 gtgtgaacca cttagcgtcc gttggatcag ggcatccaac tgcgggtcat tgagttgttt
    17221 ccaccactgc gcctgcggcc agccggagct ggcaagatga atatcatcgg ccagtttgat
    17281 ttgttccggt ttgagctgtt gatgaggcgc agaatcctta cgtaccaggg cacagccgga
    17341 aatcagcgtc gtgctgccga gaatgctgca caacagcaga cgtgaaagtt gacgattgat
    17401 cattgcgtgg ctccttgtgc ctgttccgtg gcgggcgtta atgccggggc tgtccagtct
    17461 ggcaagcggg cgaaaagctg gcagacgtgt tgctcctgtt ttaataaggt gggcaggatg
    17521 gcctgcgagg tgggtgtctc ttcaagcgtt atttgaggag aacggctgag tgcggttgcc
    17581 agaccggcag cgtatttctc cagggcgtcg gcaaactgac ctgctctgtc cggctgtagt
    17641 gcgttatcca gcgcctgcgc cgaattccag gtggcatccc aggcgtgaag gatatcgcgg
    17701 ccctgacgaa taaccgtttg cgaacgttca atcagtaagg cgcgttcttc gctgtccagt
    17761 tgacgctcca gcgccacgcg ttggcacatc tcttcacagg cattaaacgc cgcatgcaga
    17821 ccgatacgaa tttgcagata agtgcgcaga gccgtgactt cctgctggcg tgggatccgc
    17881 attactttac ttaacatacc cagcgcgcca gccagttttt gcggcagcgt gcgcgcttca
    17941 ctttcaggcc agacaaaggt gtaaatcatc gcggacacca cggtaccaat gatgataccc
    18001 agggcgcgat cgcgaatttc caccaggtcg tacactgggc caaaaacgtt ttcgagcgtg
    18061 gcgagcgcga aggtgaccac catctgtgtg ccgatataag aagagcgttc agagctggtg
    18121 gcaatccatg cgcccaacag gaaaatcggt gccagcacaa acagcaattc gacaatattg
    18181 tccagccagg gcatgaccag tagcgtgaat aacagcgcca gaatcgcgcc gcaaaaggcc
    18241 ccgccaaaac gcagcaccat cttctggtac gacgaaccga catttggatt agcgacgatc
    18301 acgcaggtca gcatacaggt gtgaatgcct tcccagtcca cgccgctgta aaaggtgtaa
    18361 cagatcaaac aggcgagcaa tgtttttacc gcgtagcgta tatagtctgg attggtaaaa
    18421 gcatcggcga ccattgatgg cggtttggcg gcgggcgttg gcggcgtatt cgggttcatc
    18481 tggcccagtt gtaacaacgt ctggcagata ttctccaggt tacattcccg tgccgccacc
    18541 gcttcacttt cactgagccg ccagtcgctt tgccagcact gaccttcagc aacggcatgc
    18601 tgcagcttgt tgatttctga ggctaatttt tgccgaaatt caataattgc ctgagaatca
    18661 gcaaaagagg tgggatcgta gcgattcagc gtcgagtaaa tgtaggttac cgttgccacg
    18721 cagctttgcc accatgcgct ttgagttcgc cagttggcat cgtccgcgag acaaaagaca
    18781 ttcagttttt gcagcgccag cgcctctctt tcaatccgcg tttcgggtag cggtgcgagg
    18841 ctgtccgtca ggtggctaat ggcatcatca agccgatcat taagcgcttg atgcatttgc
    18901 gtaatggcac gattgggaaa ccacagcacg ccgattaacg tcatcagcaa ggttggatag
    18961 aggccaacaa cgatacacca cagcgttaag cgcacgacta cttccggata gtcgagcatg
    19021 gcggggaagg tttgcccgta aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg
    19081 cgcatcaaaa acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt
    19141 tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc
    19201 acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc
    19261 actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc
    19321 aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac
    19381 tcttcatgaa agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc
    19441 attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag
    19501 gtttatcgac catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac
    19561 gagacacttt tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc
    19621 caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg
    19681 cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc
    19741 agtgacgagt gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg
    19801 aggttttgag ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat
    19861 gtagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc agcgcatcga
    19921 cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatccgctt
    19981 ctgcggcgcg ctgcgccgtt cttgcacggt caacgtcttc tgctgagaca aaaccctctt
    20041 tcagtaatgg ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt
    20101 tttctaccgt ggcattaacc gagtcggcac caaactgttg ggcgtcaacg ctacgctggg
    20161 tgagcataat ttgcttatcc agcgccgcga gggaggcttc ggctttcgcc aggttggctt
    20221 cgtacgggcg cgggtcgatg cggaacagca aatcgccctg tttcaccgcc tggttgtcgg
    20281 tgaccgccag ttctacaatg cgaccgctga cttccggcac cacatcaatg gtatctgctg
    20341 acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg
    20401 caaccagcgc caacgcaacc actaacagag cagggaattt actgcgaggt gcttttttcg
    20461 gcgtactttc catgagcgcg tctcaaatag attagaaaaa tgccagccag aacagcatgg
    20521 cgtagagagc aaaaagggcg gtataaataa ttccgacaaa tgccagattg atatttgccc
    20581 g
//
LOCUS       DAZPEU010000060        20122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPEU010000060 DAZPEU010000000
VERSION     DAZPEU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.060"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            88..864
                     /gene="exuR"
                     /locus_tag="ACHU2L_001959"
     CDS             88..864
                     /gene="exuR"
                     /locus_tag="ACHU2L_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR1356826.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1104..1187
                     /gene="yqiM"
                     /locus_tag="ACHU2L_001960"
     CDS             1104..1187
                     /gene="yqiM"
                     /locus_tag="ACHU2L_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR1356827.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1209..1871
                     /gene="yqjA"
                     /locus_tag="ACHU2L_001961"
     CDS             1209..1871
                     /gene="yqjA"
                     /locus_tag="ACHU2L_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR1356828.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1875..2258
                     /gene="mzrA"
                     /locus_tag="ACHU2L_001962"
     CDS             1875..2258
                     /gene="mzrA"
                     /locus_tag="ACHU2L_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR1356829.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2405..2759
                     /gene="yqjC"
                     /locus_tag="ACHU2L_001963"
                     /pseudo
     CDS             2405..2759
                     /gene="yqjC"
                     /locus_tag="ACHU2L_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2797..3102
                     /locus_tag="ACHU2L_001964"
     CDS             2797..3102
                     /locus_tag="ACHU2L_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR1356830.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3105..3509
                     /locus_tag="ACHU2L_001965"
     CDS             3105..3509
                     /locus_tag="ACHU2L_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1356831.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3499..3798
                     /locus_tag="ACHU2L_001966"
     CDS             3499..3798
                     /locus_tag="ACHU2L_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR1356832.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3984..4280
                     /locus_tag="ACHU2L_001967"
     CDS             3984..4280
                     /locus_tag="ACHU2L_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1356833.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4350..5336
                     /gene="yqjG"
                     /locus_tag="ACHU2L_001968"
     CDS             4350..5336
                     /gene="yqjG"
                     /locus_tag="ACHU2L_001968"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR1356834.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5629..5997
                     /locus_tag="ACHU2L_001969"
     CDS             5629..5997
                     /locus_tag="ACHU2L_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1356835.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="ACHU2L_001970"
     CDS             complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="ACHU2L_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR1356836.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7205..7906
                     /locus_tag="ACHU2L_001971"
     CDS             7205..7906
                     /locus_tag="ACHU2L_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR1356837.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7929..8093
                     /gene="yhaL"
                     /locus_tag="ACHU2L_001972"
     CDS             7929..8093
                     /gene="yhaL"
                     /locus_tag="ACHU2L_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR1356838.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8305..9615)
                     /gene="cyuA"
                     /locus_tag="ACHU2L_001973"
     CDS             complement(8305..9615)
                     /gene="cyuA"
                     /locus_tag="ACHU2L_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR1356839.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9643..10974)
                     /locus_tag="ACHU2L_001974"
     CDS             complement(9643..10974)
                     /locus_tag="ACHU2L_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1356840.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="ACHU2L_001975"
     CDS             complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="ACHU2L_001975"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1356841.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12685..13074)
                     /locus_tag="ACHU2L_001976"
     CDS             complement(12685..13074)
                     /locus_tag="ACHU2L_001976"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR1356842.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="ACHU2L_001977"
     CDS             complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="ACHU2L_001977"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR1356843.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="ACHU2L_001978"
     CDS             complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="ACHU2L_001978"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR1356844.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="ACHU2L_001979"
     CDS             complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="ACHU2L_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR1356845.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="ACHU2L_001980"
     CDS             complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="ACHU2L_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR1356846.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="ACHU2L_001981"
     CDS             complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="ACHU2L_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR1356847.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5205 a         5270 c         4812 g         4835 t
ORIGIN      
        1 aatcatcaaa tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc
       61 gtccgcattc cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa
      121 caacttgctg ctgacctgaa agagcgcatt gaacagggcg tctatctggt gggtgataaa
      181 ctgcctgccg aacgctttat tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa
      241 gccatcatca tgctggaagt tgaaggctat gtggaggtgc gtaaaggttc aggtattcat
      301 gtggtttcca accagccgcg tcatcagcag gcggctgaca ataatatgga attcgccaat
      361 tacggtccgt ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc
      421 gcggcaactc aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg
      481 cgcggcgaac aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc
      541 ctggcgacgc agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc
      601 cataacccgt actggaaaaa actacacgaa cacattgatt cccgtaccgt tgataactgg
      661 tgtgatgacc acgatcaaat tctcaaggcg ttaattcgca aagatcctca tgctgctaag
      721 ctggcaatgt ggcagcatct ggaaaacacc aaaatcatgt tatttaacga aaccagcgac
      781 gatttcgagt tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat
      841 acggccacca gcggcagcaa atgaaattcc tgtccgacag gcgctgcaat gcgcctgttt
      901 gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc
      961 ccctgcaatc agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga
     1021 cctttgttac aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacgt
     1081 tttaacaggg aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca
     1141 gcaataacat tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg
     1201 aataatgaat ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa
     1261 ccctggccaa tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc
     1321 ttgaaaatgg cttgcttccg gcggcctttt taccgggcga cagtttactg gtattggtcg
     1381 gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg
     1441 ccgccagcct cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca
     1501 ccgtacaaaa ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt
     1561 ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt tattgcattt gtcagaacac
     1621 tgctgccgac gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact
     1681 ggatgagcgg tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa
     1741 cgccggtatt tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg
     1801 tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa
     1861 atcgggggta agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca
     1921 ctgttttact tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt
     1981 ctacgctggc gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct
     2041 ggcatcacct tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc
     2101 tgttaattac atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa
     2161 cattgcccca tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc
     2221 tgacccggtt acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat
     2281 cactcacttt ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta
     2341 ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc
     2401 aagaatgaaa taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta
     2461 tgccactacc ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga
     2521 aaaacaccaa aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc
     2581 caactgttca gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga
     2641 tgaggtggcg gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgatag
     2701 aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac
     2761 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
     2821 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
     2881 ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
     2941 cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg
     3001 gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg aattggcgct
     3061 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
     3121 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
     3181 ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta
     3241 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
     3301 tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga
     3361 ttgccaccac cgtggtgttg ctgctactgg cactgattgg cggtatctgg acgttacgta
     3421 aatcgcgtaa atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac
     3481 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg
     3541 cttagccaga tccagcaaca acggctggat atttccgcca gtagtcgtga atggctggag
     3601 gcaacaggcg cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg
     3661 gttggcagta gagtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg
     3721 gccagacgcg gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag
     3781 caacagcttc gcggttaact tccctctggc cggagcaatt ccggccttat ccatcaaatt
     3841 ttttgaatat ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc
     3901 tctccatcga cagcaacgac gctaatagcg cgcccttgca caaaaaaaca atcagcagcc
     3961 tgagtggcgc agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc
     4021 acattttaat gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg
     4081 gtacccaaca atatatggaa gcaatgggtg tcccgggttt tatgctgctg acggcatttt
     4141 tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa aacctgacaa
     4201 tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc atcggccgcc
     4261 tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga ggaggaagct
     4321 cctcgttttt gctattggag gagagaaaaa tgggtcaact gattgacggc gtctggcatg
     4381 acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct tccgcatttc
     4441 gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt tttatcgcag
     4501 agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga
     4561 tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg aacccgctga
     4621 tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga gatacgctct
     4681 atcaacatga atttctgtat cagctttatc tccacgccga tccacactac agcggacgag
     4741 ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac gaatcagcag
     4801 aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg ggtgattact
     4861 acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat gacaccgtta
     4921 acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac gaggcggtgg
     4981 cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat cgttacctga
     5041 ccggcaacca gctaaccgaa gccgatattc gcctgtggac tacgctggtg cgttttgatc
     5101 cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac ctgaatctgt
     5161 atggcttcct gcgcgatatc taccagatgc cggggatcgc cgaaactgtc aatttcgatc
     5221 acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg attatttcaa
     5281 ttggtccgtg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa
     5341 aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat gtcggatgcg
     5401 gcgtgaacgc cttatcaaac ctacaaagcg tttatattca atatgttaca atgaccgcgt
     5461 aggccagata agcgtagcgc atctggcgat tttgcgcttg ccatcagtct tagcgcgcct
     5521 tcacggcact ttattaattc cttgaacgaa tatttactgc catttcattc acgtctattc
     5581 ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat ggactggtat
     5641 ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg
     5701 atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga taaaatgtta
     5761 ggctggcaac gcgctggtgg cgaaggcatc ctgacgataa tctacggtat tctggtgttt
     5821 ttaccgtggt gggcggttca gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg
     5881 gcactgctat tcttaatccc gtttatcggc tggcttatta tcatcgtctt taactgccag
     5941 gcgggtacgc caggcgaaaa ccgctttgga ccagatccaa aactggaaca agaataatga
     6001 ttaagcacct tgtaaaaggt gcttcaaatt gttgacaaag tgcgctttgt ttatgccgga
     6061 tgcggcgtaa acgccttatc cagcctacaa attgccaaaa ttcaatatat tgcagtgact
     6121 gcgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgtcagca gtcagaagca
     6181 ccttgtgaaa ggtgcttaat agattatttt ccagcgaaca actttggaat ttcccgcaga
     6241 caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctcg
     6301 ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc
     6361 ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc
     6421 gtgctcaccg ttaagcgcgg ctgtttgtcc agcagctgta cggtcaacac cgggcgctca
     6481 cgagcggtat ccgctaccgc aattccacga tatttcacac gcgtcacttc agataacggt
     6541 tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag
     6601 agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacgata
     6661 tccgcccgcc cctgctccag ccgttcccac gcccccgcca gcacttctgt gatgattgcc
     6721 agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt
     6781 accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag agcttccgca
     6841 tcagtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag caacatcctc
     6901 ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct
     6961 tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgct
     7021 gccgccgcaa aactgccccg gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt
     7081 aatgcccttt ctttggccat ttcgttctca ttcaggaaat ttgaacatac cgggcagaat
     7141 atctggctaa caatgtagcg tccagcccct taccataaaa ggaagtaaag agaggtcaag
     7201 aattatgatt actacccgaa ctgccaggca gtgtggacaa gcagactacg gatggttgca
     7261 ggcccggtat actttttcct ttggacacta cttcgacccg aaattgttag gctatgcctc
     7321 cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta
     7381 tcccaaagtc gatattttaa atgtgattct ggatggagaa gcagagtatc gcgacagcga
     7441 aggcaatcat gttcaggcca gcgccggaga ggcgttgctg ctctctaccc agccgggtgt
     7501 cagctatagc gaacacaatc tcagcaaaga caaaccgtta acgcgaatgc agctttggct
     7561 ggatacctgc ccgcagcgag agaatccgct gattcaaaag ctggcgctta atatggacaa
     7621 gcagcaatta atcgcctcgc cagagggtgc gatgggaagc ctgcaattac gccaacaagt
     7681 gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg aatttccagt tgcatgggcc
     7741 acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg cttacgcatc atgaagagaa
     7801 agcggcgcta acctgcggtg atggggcgtt tattcgtgac gaggctaaca ttacgctggt
     7861 tgccgattcc ccactgcgcg ctttgctgat agatttgcct gtctagttgt ttttacaggg
     7921 agatgatgat gagtaaaaaa tcgaccaaaa agcgccagcc agcgaagccc gtggtggcga
     7981 aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga gatgttgagc gagctggaag
     8041 ctatcgtcgc ggatgctgaa acgcgtttag ccgaggatga agctaccgcg taaagacagg
     8101 ttgccggatg cggcgtaaac gccttatccg gcctacaaaa cacaacaaca tcaacaaatt
     8161 aaacaccccg taggccggat aagatgcgcc agcatcgcat ccggcatcag gtacaaaaca
     8221 caacaacatc aacaagttga gcaccccgta ggccggataa ggtgcgccag catcgcatcc
     8281 ggcacaatac tcaaaacctg gcgtttatct ggccttgctc gccataatct cgataatctg
     8341 ccgatccgtt tgctgcatcg aatggcttgc taacgcacac aggttggcaa tcgactgctc
     8401 aacatcatgc gccacgatcc cttcattgcc ggtcacggcg gtatcatcca gcgccattaa
     8461 caccgctttc cacgcagccg aagcactggt cgaaaccttc atcgcgcagc tgttcgacgc
     8521 accatcgcaa atcatgccgc tgacatcgcc gatcatactg ctgatcgcca tcgagatagt
     8581 ttcataacgc ccatccacca gccatgccat cccggcggcg gcccccattg ctgcggtcgt
     8641 tgcggcacaa agtgcagaca aacgcggtaa ctggttatgg atgtaaattg cgctcaaatg
     8701 agaaagcatc agcgcacgcg ccagtcgttc atcatccgct ccgaagtgtt ctgctacaac
     8761 caccacaggc attattgcag tgatcccctg gttacccgag ccggagttac tcatagccgg
     8821 aagcgtagcg ccgcccatac gcgcatcgga tgccgcgctg gtacgaatca caatggatga
     8881 agagagatct ttcgccagca aaccgcgctc gcactgtttt tccagcgtcg cgccaatatg
     8941 cagcccccac ttaccgctca aaccttcctg cgataacgca caatttagct tcgcggaatc
     9001 gagaataaag cggatcgccg caaatgggac ttcattgacg aacttcagga tctcagccag
     9061 cgtcgttctg gaaagcaccg tcagcggcga ctcttgctcg ccctctgcca cacacgcctg
     9121 gtgggtaaac accacaccat tgtgcgtctc aatatgcaca atgttggtat gcccgccgac
     9181 gatggtgaca cacgcccact tctcaccgtt ccagactttg gcgcgtgaga agaggatttc
     9241 attgcaaggt tcctggatct taacagagac tttccccgcc gccagcagtg ctttggcatc
     9301 ggtaattgcc tgcgcagttg cgtctttcag cacttccagc ccggcgttgg catttccacc
     9361 taacgccccc agcgccgccg caatcggcag ccccaccatt cccgtgccgg gaacggtgac
     9421 gcccagaccg ttcttcatca gatttggcga aacccaggct tctacacgtt caaccggacc
     9481 ttccagttct gccgcagcaa ccgccgccgc cagcgccagt gaaatcggtt cagtacatcc
     9541 cagcgccggt tttacttcct cctgaacggc gaggatgtaa cgctgccata acggatttaa
     9601 agtcgaatca aacatattaa aaaccttaaa atttcaggta aatcaagaaa atgccaggaa
     9661 cagagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca tccctttgta
     9721 tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc ctaccatgcc
     9781 gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta cgatggcgct
     9841 ccaggccagc aaaatggcga aaatcatgat gccgcgctga acaagatttt cgttaatctt
     9901 ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg cttcgcgaaa
     9961 gcctaaatag acgccaaaga atgcagtcat gacagcaaag atattgagaa tgacgctgac
    10021 cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg ccagcgcgga
    10081 aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg tgaacgacac
    10141 ggcgtagaaa aagacggtga caaacaaaat gccaaacgcg atattcattg cccgcaatgc
    10201 tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca ccatcgggct
    10261 taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa tggcgttttt
    10321 caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca ttcccaccat
    10381 cgacacgccc agcgcagcga ctaccagcag cttggtcagc accatgtcgg tcgaaatttt
    10441 gaataacaat ttctcgccgc gtgaagagat cgccaccaga atgcaaatca gtaccagacc
    10501 ataaaaggga ctgtctgaca gcaacccttc cgtcacgccg aaggtatgca gataggaagc
    10561 actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca ttacgaaata
    10621 gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctaatga cactcgggta
    10681 atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa acagatacat
    10741 tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca aaccgacctg
    10801 caccgggaga aaaacaatcc ccgcgccaat cgccatcccg atactcataa tcacccagcc
    10861 ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca ttccagcacg
    10921 acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt ccataatttc
    10981 tcgctcaata ttttgtaggg cttattattt ttttccgagc cgcatcaagg cgatatgcgg
    11041 tctacgtttt tgcaggcgag ttattagaat aaagaaatga tacgcgcgag gtcagagaaa
    11101 atcttcacaa tcaagacttt aaaataacgg agatggataa gaattttcta ctaaattaat
    11161 cacaagagaa tattcattct ctatttgcgg cagatcacaa aaaaaaggtg cacatttgtg
    11221 cacccaagga tgaaagctga cagcaatgtc agccgcagac cactttaatg gccagtcctc
    11281 cgcgtgatgt ttcgcggtat ttatcgttca tatctttgcc ggtttcatac atcgtctcga
    11341 tcactttatc gagtgaaaca cgcggtgccg aggtgcggcg catcgccatc cgcgcggcgt
    11401 ttactgcttt cacggcatta atggcattac gttcaatgca cgggatttgt acctgtccgg
    11461 caaccggatc gcaggtcagc ccaaggttat gctccatcgc gatttccgcc gcattgcata
    11521 cctgcgccgg actgccgccc agtagttcag ttaaccctgc cgctgccatt gaacaggcca
    11581 cgccaatctc cccctgacag ccgacctccg cgccagagat ggaggcgttc attttataca
    11641 gcgcgccaat agcccccgct gccagaaaat agcgggcaat tgaccgctcg tttaccggac
    11701 gacggaactt atcgtaataa gccagcactg ccggaataat gccgcacgca ccgttagtcg
    11761 gtgccgttac cacgcgcccg ccagctgcgt tttcttcact aaccgccagc gcgtacatgt
    11821 tgatccagtc gatgacattc atcggatcgt tagagatgtt atcgctagaa accagctgac
    11881 gacgcagcgc tacggcacga cacggcacat tgagcggacc aggcagcacg ccttcagtgt
    11941 tcatcccgcg ttcaataccg tcatgcatca cttgccagat acgggcaaac cctgcgtcaa
    12001 tttccgcttt gctgcgcagc gccagctcgt tgtgcatcat caggccagat atagacaggc
    12061 cgttgtaatc acacattttc agcagttcac ctgctgagtg gaaatcgtaa ggtacggacg
    12121 tttcgacatc gtgcgacagg ccgaagtgtt cttcttcgac aataaacccg ccgccgacag
    12181 agtaataggt tttacttaat agcgcttcct gtcccttcca ggcagtgatc cgcattccgt
    12241 tctcatggcg aggcaacatt tcgggatgga agataatgtt ctttgctaca ggaaaatcaa
    12301 caatatgcgc accagatgcc actggcagcc gcccgctgcg cgttactaac tctataaatg
    12361 cagggatctc atcaatgaca acatcctgcg gactgtttcc tgccagtccc atgataatgg
    12421 cgacatccgt ggcatggcct ttgcccgtca gtgacaacga cccgtacaga tcgaccacaa
    12481 tatggctcgt cgcggttaat aagccgctac tttccagccg atcaataaaa ctttttccgg
    12541 cattcattgg ccccacggta tgcgaactgg agggaccaat cccaattttg aaaatatcga
    12601 atgcactaat catatccaca ccctcggatt gccgttcagt gaagtggagc ggaacgacct
    12661 tgcgaccgtc ccgctcacga ggctttacgc actacgtact gcgatagctt caatttccag
    12721 cttcacatct ttcggcaaac gcgcaacctg cacacagctc cgggtcgggt aggtcgcctg
    12781 atgctcatcg aagaactgct tatagacttc attgatggtg gcaaaatcat tcagatcggt
    12841 gatgaacacg gtcatcttga tgatatcgcc cacgctcagc ccggcagcaa ccacgatcgc
    12901 tttgacgttt tcgaggctta aacgcgcctg atcttgcaca tcagccggga tctcaccggt
    12961 ctgcgggcaa accggaattt gcccggaggt gaagaccatg ctgcctaaat caacgccctg
    13021 aacataaggg ccgattgcgc ctggggcacg ttgcgtttcg ataatctttt tcatacatcc
    13081 tccggcgtca gagcgcctgg gtaaaagtac gtgaaataac atcctgttgc tgttcacggg
    13141 tcagtgcgtt gaagcgcacg gcgtagccag agacacggat tgtcaggtta ggatattttt
    13201 ccgggtgctc gatggcatcc agcagcattt cccgattcat tacgttgacg ttgaggtgtt
    13261 gaccgccttc gacatccgct tcgtggtgga aatacccatc cagcaggccg acaaggttgg
    13321 ttttacgtac tggatcttct ttgcccagcg ccgcaggaac gattgagaag gtgtacgaga
    13381 tcccatcttt ggcgtaggtg aacggcagtt tcgccaccga cgtcaatgag gccacggcac
    13441 ctttgcggtc acgaccatgc atcgggttag cgcccggcgc gaacggtgtt ccggcgcgac
    13501 gaccgtccgg cgtattaccg gttttctggc cgtacaccac gttagaagtg atagtcagaa
    13561 tcgactgggt agggacggcg ttgcgatagg ttggcagcgc tttaattttc ttcataaagc
    13621 gttcaaccag gtcgcaggca atgctgtcta cgcgctcgtc gttgttgccg tactgcggat
    13681 attcaccgtc gatttcaaag tccaccgcca ggccgttttc gtcacggatt ggtttcacgc
    13741 gggcatattt gatggcagac agggagtccg tcgccaccga caggcccgcg atgccgcatg
    13801 ccatagtgcg atagacatca cgatcgtgca gcgccatcag cgaagcttcg tagctgtact
    13861 tgtcgtgcat gtagtgaatg atattcagcg cgctgatgta ctgcaccgcc agccagtcca
    13921 tgaagtgatc gaggctgtcc atcactttgt cgtaatccag cacgtcgtcc atcagcggtg
    13981 ctgttttcgg cccgacctga atcttcagct tctcgtccac cccgccgtta attgcgtaga
    14041 gcagcgtttt cgccaggtta gcgcgtgcac caaagaactg catttgctta ccaatcacca
    14101 ttgggctgac gcagcaggca atcgcgtaat cgtcgctgtt gaagtcagta cgcatcagat
    14161 cgtcattttc atactgcaag gaagaggtga cgatcgacac ctgcgcggca tattttttga
    14221 aggcaatcgg taattcttcc gaccaaagaa tggtcaggtt aggttccggt gccggcccca
    14281 tagtgtgcag ggtgtgcaaa tagcggaagg agtttttggt caccagcgta cgaccgtcca
    14341 gccccatccc gccgatcact tccgtcgccc agattgggtc gccggagaac agcgaatcaa
    14401 attccggtgt acgcaggaag cgtaccatac ggatcttcat gatgaagtga tcgatcagtt
    14461 cctgtgcctg ctgctcattg aggacgccag ctttaaagtc gcgctcaatg tagatatcca
    14521 ggaacgatgc cgtgcgcccc agcgacatcg cgccgccatt ttgcgatttc actgccgcca
    14581 gataagcgaa gtagagccac tgcaccgctt cctgtgcatt ctgcgccggg cgagagatat
    14641 caaagccata tttcgccgcc atttcctgaa tctgcaacag cgcatgacga tgctctgcca
    14701 gctcctcacg cagacggatg gtggcttcca gatcctcgcc tttttccaga cgagactgga
    14761 gatcggcaaa ttgcagttcg cgttcacgca ccagataact gatgccatac agcgctacgc
    14821 ggcgatagtc accgataatg cgcccacgac catagccatc tggtaaaccg gtcagcacgc
    14881 cagatttacg gcagcgcagc atatccggtg agtaaacatc aaatacgccc tggttatggg
    14941 ttttacgcag atcggtaaac agatattcaa attcactgtc catttcacgg ccataggcgt
    15001 ggaatgaact tttaatcata ttaatgccac cgaacgggtg tagcgcacgt ttcaacggcg
    15061 catccgtttg caggccaaca attttttcca gcggctggtt aatatatccc gcatcatgag
    15121 cggtaattgt ggtggcaata ttggtatcga aatcaaccgg cgcgtgggtt gcgttttcga
    15181 tacggatgcc ttccattacc ttttcccaca attccgtggt ggcaggcgtc gcttcggcga
    15241 gaaaagattc atcgccttca tacggtgtat agttatgttg aataaaatcg cggacattaa
    15301 tttcgctttt ccagtccgta cctttaaagc caagccatgc gtcagcgtac agcttatcgc
    15361 tggtatcaat atctaccttc atgaaaaata atctctctac aatacttcaa ctaaattatg
    15421 caaattctgc gggcgcgtta actttaccta aatgaatggc atccaaagca atcatttttt
    15481 cttcgttagt cggaataacg acgcaaatga catgcgcatt ttcactggaa acaattcgct
    15541 cgccaaaaga gttagaacga ttattcattt ctgtatcaat ctctacgcct aatacagcca
    15601 aatgttccat gaccagacga cgaattaaac ttgaattctc tcctattccg ccggtgaata
    15661 taattccatc cagccgacgt aatgaagctg catgtccggc aatatgacgg gcaattcggt
    15721 gaacaaaggt tttaattgcc agttgcgcgc gttcgtgacc ttcatgccag gctttttcca
    15781 gaacacgtaa atccgaagaa agaccggaaa tacctaataa tcccgactct ttattcacta
    15841 cgcgttccag gtcacccagg ctctggttgg tttggctggc gacccaggac atcgcaccaa
    15901 agtcgacatc gccactgcgg gtacccatca tcaagccttc cagcggcgtc attcccattg
    15961 aggtgtcaac actctgaccg ttgcgaactg cgcagattga cgcgccattg ccaagatgcg
    16021 ccacaaccag gccggagtca tcttccgcca gattcagcag cgaatgtgcg cgctgggaaa
    16081 catagcggtg cgacgtgccg tggaaaccat aacggcgtac acctaactct tcataatatt
    16141 tccacggcag gccgtataaa taagcttccg gagccatcgt ctggtggaaa ctggtatcaa
    16201 ataccgccac ctgagttacg cccggaaata attgctgcgc cgattcaata ccacttaaat
    16261 ttgcgtaatt atgcaggggt gccagtggag aaacgcgacg gatattatca atgacttcat
    16321 cggtaataat ggcggactcg gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta
    16381 aggccacgct gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac
    16441 cttcgtagct gtggtgagcc agcggtgctg gctctccccc atttacggat aagaatgcat
    16501 tttccgagtt aataccgtcg gcaatacctg acattaatac ttcacaatcg ctggcatcga
    16561 gtacggaaaa cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat
    16621 tcatctcttt tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggatg
    16681 gtcagcagac caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga
    16741 gacggcgctt tacggatagc atacatcggc aacaggcaca gcagggatgc gataatcggt
    16801 gcgcccatgg cttcaatcag gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg
    16861 gagcccatga tgaagatcat gctgagagta ttcagtttac ccagcgacac tttggttttg
    16921 tcacctttat aaccgaactt cagaatcaga ccattcaagc cttccagcgt ccccagatag
    16981 tgaccgaaga aagatttgaa gatagccacg agtgcgatga tggaagccgc atattccagt
    17041 gtaatcgcga acgttgtttt cgtaccggtc atggacgcaa agtggttagc cagataagaa
    17101 agcactggaa tattctgcgc tttggcttcc gccatgttgg ccggagacag agtaaacagg
    17161 cagctaaagg caaagaacat caccactgca accatcagca tgctggcacg agaaatgatt
    17221 tgggaacatt tacgttcggt gaagtcgcga ccgaagtctt tctcatactc ttcacgttta
    17281 gaaaccacga aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc
    17341 cccagccaga cagtgatcag gataccgtca tgaccggtta acgacagcga accgaggtca
    17401 acctggtcga taactgcaga gttccagtaa gggatcagcg acagagaaat cagcaccagg
    17461 ctggcgataa acggccatac caggtagctc atcactttaa ccatcagatc cttaccaaac
    17521 cagatgacga aagccatcag cagcaacagg aacagcgcca caaagccgcg attcagcggt
    17581 gcaaatccga gctggttttc ccagaacgtc ataaaggtat tggtaatagt aacgccataa
    17641 atccacagca gtgggcaaat cgcgaagaag tacaggaacg tgataaccac gccgccagtt
    17701 ttaccaaaat gctcttccac cgtttccgta atgttgccgg aagggttaga gccagaaaga
    17761 cacagacgcg ccagcgcccg gtggcaataa aatgcgatgg ggtatgccaa taccaacatc
    17821 agaagaatcg ggatcagtcc gccaaaacct gcgcggatag ggaagaacag caccccggcg
    17881 ccgattgccg taccaaacaa gcctaacgtc catgtggtat ctgatttacg ccaggacgat
    17941 tgttttgtct ggctggatac aatgctatct gaagtactca tatcctatcc tcaacgaatt
    18001 aattaagcgt caacgaaacc ggtgatttga gagacgcgag aaagatcgat attgccgccg
    18061 gaaataatac tgacggtttt tctattttga atatattggt ctaatttacc gcttaataat
    18121 gcagcacatg ccagagcgcc tgcgccttcg gtgacgactt tattgcgctg aattaaggca
    18181 atcatactgt ttctgatttc gtcttcgctg accagcacga tgtcatcgac taattcacga
    18241 acgatttcgt aagttaaatt acccgggcgg gagacatcac aaccatccgc cagggtgccg
    18301 gtagttcggt gcgtggttat ttctccggag tggaaagaag ccgccatgcc gtgaacgttt
    18361 tctgactgta cgccaataac acgaatggtc gggttaatag atttaattgc caccgcaata
    18421 ccagcaatta aaccaccacc accaattggc acaatcacgt tatcgacatc atagagatct
    18481 tccataattt ccagaccaat cgttccctgg ccagcaatca ctttcggatc atcgtaaggt
    18541 gggataaaaa tacgaccttc catttcgaca atttcgctca ctttagcgat agtgtcgttg
    18601 aagttatcac catgcagaac gacttctgcg gagtagtcgc acgttgccgc tactttggat
    18661 tttggcgcac cttttggcat caccacttta ccgtcgatac ccagcatcgc gcaggagagc
    18721 gaaacccctt gcgcatggtt gcccgcagaa caggccacca cgcctttgcg tttttccgca
    18781 tcggtcagtg aacttaattt attaaatgcg ccacgaattt taaatgaacc cgtacgctgc
    18841 atattttcaa acttcaggaa tatttcacct ttgcaacgtt cactaaaata gttggagcga
    18901 ggcatgcctg ttttataaat tcgcccagcc agtcgttgtt tcgcttcaat aatatcatca
    18961 atagcaaccg gcagatcgta tgtaatatgc attataaaac ctcttcgcct gaattaaata
    19021 tgtaggtaac cgtaaccgac acctgcaaaa cagacaggtg gattatttat gtttaaataa
    19081 aaacaaacaa ctaaccaact tctattaatt gccttcgtct acacccatta taagatgaat
    19141 actctttggc taattccacc aaaaccgatg ctgctttttt aatacgataa tttttcgacc
    19201 ataccgcggc atattgtgcc acaggtaatg tttcttcaac cggaatagta ataaattgat
    19261 tagaaccaaa aggtgacgtc atatcacaag gaattacagt taagaaatca gcattgagaa
    19321 caagattata aattgtcacg actgagtcgg ttttaacgat gttttcaata ctgatgccat
    19381 ttctttgtaa cgtagtaagc agttcgctgt agtaccccat attagtttgt ggcaacaccc
    19441 actgttcgtt cttcaacgac tccagcgtgg tggtgccggt gcatgttcgg gacttactgg
    19501 ctaccagcac aaactcggac tcgaacagcg gctcaacatg taaatcctga agcttcattt
    19561 ctgcacttaa cgtaccaatc gcaaaatcca gtcgaccgtc gcggattgcc ggtaagaacg
    19621 aagacagttg cgcttcatac atagaaacct gcgctttcgg gaacacctct ttgaacttgt
    19681 tgatcatccc tgacataaaa gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca
    19741 ccaccgcttc agaagacata ccgcttatct cattaaccat atttttcatt tcacgggtaa
    19801 tggattcgga acgggagagt aacaattgac cggcaggtgt taatgttaca ccggtatttt
    19861 tccgcaccac taattccaca ccaaaataat cttcaatatc gttaatgatt ttactgacgg
    19921 ccggttgagt taaccctaat tcttttgcag ccgagccgat agagccactt ctaatgactt
    19981 cctgaaagac taccaggtgc tgcgttttcg gaagaagaat agtgctcata acgacctacg
    20041 ttaattacct cattgacggc atgaagtgta tcaaaatgaa ataaacagga tatgtgcgac
    20101 cactcacaaa ttaactttca tt
//
LOCUS       DAZPEU010000061        20084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPEU010000061 DAZPEU010000000
VERSION     DAZPEU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.061"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACHU2L_001982"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACHU2L_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR1356848.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHU2L_001983"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHU2L_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFR1356849.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHU2L_001984"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHU2L_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR1356850.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACHU2L_001985"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACHU2L_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACHU2L_001986"
     CDS             1631..2341
                     /locus_tag="ACHU2L_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR1356851.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHU2L_001987"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHU2L_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR1356852.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACHU2L_001988"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACHU2L_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6480
                     /locus_tag="ACHU2L_001989"
     CDS             4282..6480
                     /locus_tag="ACHU2L_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR1356853.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="ACHU2L_001990"
     CDS             complement(6449..6889)
                     /locus_tag="ACHU2L_001990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1356854.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHU2L_001991"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHU2L_001991"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR1356855.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="ACHU2L_001992"
     CDS             7607..8305
                     /locus_tag="ACHU2L_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR1356856.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="ACHU2L_001993"
     CDS             8534..9742
                     /locus_tag="ACHU2L_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1356857.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="ACHU2L_001994"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="ACHU2L_001994"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR1356858.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="ACHU2L_001995"
     tRNA            11849..11925
                     /locus_tag="ACHU2L_001995"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="ACHU2L_001996"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="ACHU2L_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR1356859.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="ACHU2L_001997"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="ACHU2L_001997"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR1356860.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="ACHU2L_001998"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="ACHU2L_001998"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR1356861.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="ACHU2L_001999"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="ACHU2L_001999"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR1356862.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="ACHU2L_002000"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="ACHU2L_002000"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR1356863.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVELIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="ACHU2L_002001"
     CDS             complement(18540..19811)
                     /locus_tag="ACHU2L_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1356864.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4811 a         4974 c         5275 g         5024 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagctcattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agca
//
LOCUS       DAZPEU010000062        20082 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPEU010000062 DAZPEU010000000
VERSION     DAZPEU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.062"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            77..1408
                     /gene="argA"
                     /locus_tag="ACHU2L_002002"
     CDS             77..1408
                     /gene="argA"
                     /locus_tag="ACHU2L_002002"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR1356865.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(1470..3296)
                     /gene="recD"
                     /locus_tag="ACHU2L_002003"
     CDS             complement(1470..3296)
                     /gene="recD"
                     /locus_tag="ACHU2L_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR1356866.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(3296..6838)
                     /gene="recB"
                     /locus_tag="ACHU2L_002004"
     CDS             complement(3296..6838)
                     /gene="recB"
                     /locus_tag="ACHU2L_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1356867.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(6831..9719)
                     /gene="ptrA"
                     /locus_tag="ACHU2L_002005"
     CDS             complement(6831..9719)
                     /gene="ptrA"
                     /locus_tag="ACHU2L_002005"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR1356868.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(9895..13263)
                     /gene="recC"
                     /locus_tag="ACHU2L_002006"
     CDS             complement(9895..13263)
                     /gene="recC"
                     /locus_tag="ACHU2L_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR1356869.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(13276..13626)
                     /locus_tag="ACHU2L_002007"
     CDS             complement(13276..13626)
                     /locus_tag="ACHU2L_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1356870.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(13611..14018)
                     /locus_tag="ACHU2L_002008"
     CDS             complement(13611..14018)
                     /locus_tag="ACHU2L_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR1356871.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(14015..14578)
                     /locus_tag="ACHU2L_002009"
     CDS             complement(14015..14578)
                     /locus_tag="ACHU2L_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1356872.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(14569..15039)
                     /locus_tag="ACHU2L_002010"
                     /pseudo
     CDS             complement(14569..15039)
                     /locus_tag="ACHU2L_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(15223..16017)
                     /gene="thyA"
                     /locus_tag="ACHU2L_002011"
     CDS             complement(15223..16017)
                     /gene="thyA"
                     /locus_tag="ACHU2L_002011"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1356873.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(16024..16899)
                     /gene="lgt"
                     /locus_tag="ACHU2L_002012"
     CDS             complement(16024..16899)
                     /gene="lgt"
                     /locus_tag="ACHU2L_002012"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR1356874.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(17050..19296)
                     /gene="ptsP"
                     /locus_tag="ACHU2L_002013"
     CDS             complement(17050..19296)
                     /gene="ptsP"
                     /locus_tag="ACHU2L_002013"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1356875.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(19309..19839)
                     /gene="rppH"
                     /locus_tag="ACHU2L_002014"
     CDS             complement(19309..19839)
                     /gene="rppH"
                     /locus_tag="ACHU2L_002014"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR1356876.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         4792 a         5595 c         5034 g         4661 t
ORIGIN      
        1 aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acaataattt caaataatca
       61 tgcaaagggg tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg gattccgcca
      121 ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga
      181 agccattgag catgagaatt tctccagtat cgttaatgat atcgggttgt tgcacagcct
      241 cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa atctggctgc
      301 gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgacgcca aaacactgga
      361 actggtgaag caggctgcgg gaacattgca actggatatt actgctcgcc tgtcaatgag
      421 tctcaataac acgccgctgc agggcgcgca tatcaacgtc gttagtggca attttattat
      481 tgcccagccg ctgggcgtcg atgacggcgt ggattactgc catagcgggc gtatccggcg
      541 gattgatgaa gacgcgctcc atcgtcaact ggaaagcggt gcaatagtgc taatggggcc
      601 ggtcgctgtt tcagtcactg gcgagagctt taatctgacc tcggaagaga ttgccactca
      661 actagccatc aaactgaaag ctgaaaagat gattggtttt tgctcttccc agggcgttac
      721 taatgacgac ggtgatattg tctccgaact tttccctaac gaagcgcaag cacgggtaga
      781 agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc gtggcgcagt
      841 gaaagcctgc cgcagcggcg tgcgccgctg tcatttaatc agttatcagg aagatggcgc
      901 gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga tggaaagcgc
      961 cgagcagatt cgtcgcgcaa caatcaacga tattggcggt attctggagt tgattcgccc
     1021 gctggagcag caaggtattc tggtgcgccg ttctcgcgag cagctggaga tggaaatcga
     1081 caaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc tctatccgtt
     1141 cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt accgcagttc
     1201 atcacggggc gaggttctgc tggaacgcat tgccgctcag gcgaagcaga gcggcttaag
     1261 caaattgttt gtgctgacca cgcgcagtat tcactggttc caggaacgtg gatttacccc
     1321 ggtggatatt gatttactgc ccgagagcaa aaagcagttg tacaactacc agcgtaaatc
     1381 caaagtgttg atggcggatt tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa
     1441 cactcgtgcg tcgcatccgg caattacgtt tattcccgcg aactaaacaa cgccgccaga
     1501 ccactgcgcc gctcagtacg agtggcgatt gccgcactta atatacgctc atcggcatac
     1561 agcgacagac gacgacgcgc gcgggtcacc gcggtgtata ccagctctcg cgttactacc
     1621 ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga actccgatcc ctgcgattta
     1681 tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc gactcggttg cacagactta
     1741 atattgccgt ccggcatcgc aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata
     1801 ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac gggcaatcat caccggtcgg
     1861 ccttcgtacc aacgagagtg cggatgacga tgaattttgc gcttctgttg cataaactgt
     1921 tcaattcgct cattcagtcc agccacgcca aacggccctt cccgcagggc gcacaaaagc
     1981 tggtactcat tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg cagcagatcc
     2041 agataacgtc cgtaacccgc aagagcttcc tcaagcatcg caatataatc ttcgccgctc
     2101 tgtaaaagtc gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt
     2161 ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct gccgaaacga
     2221 tagctttttt gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt gccggttcct
     2281 gccggaacgt gagtccccgt caggcggctt agctgcctgg cacgctcggc ggtaaagccc
     2341 gcgttggcat aagcgcagat atcgcccagc acagccccag cctcaaccga ggccagttga
     2401 tcacgatcgc cgagaaagat cactcgcgca tgatctggca aggcgtcgat cagtctcgac
     2461 atcataggca gatcgatcat tgacgcttca tctaccacca gcacatcaag atgcagcggg
     2521 ttaccggcat gatgacgtaa acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa
     2581 gtgctggcat cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa
     2641 gccttgccga gagattcggt taagcgcgcg gcagctttac ccgttggtgc agccagacgg
     2701 atacggcagc gttcgccgtc ggccatttga attaacgctg ccagcaactt cgccacggtg
     2761 gtcgttttac cggtgcctgg gccgccggaa atcaccgaga tccgccgcgt cagcgccact
     2821 gccgctgcaa ctttttgcca gttaatttca tcgcttactg gaaaaagttt gtccagggtt
     2881 tgcgccagta gagcttcatc aacctcaatg gtatgattca cctcattgaa gaagcgcgct
     2941 accgtccgct cgttacacca catgcgattc aaataaagac gatcgccaca gaggatcatc
     3001 ggcgtgggtt catctcccct gctgaccgct tgagaagcca gcaagcattc ttcccaattt
     3061 tgtagctcac cgatttcact gacacaggtc gccaacagcg gattcgacgc ctcgttattt
     3121 tccagtcgtg aaagcggcaa acagacgtgt ccctctccgg catcatgact taacagtgcc
     3181 gccgcgaggg tgacggcagg atgttcatct cccgccacgg tcagggcaaa ttgcacatcc
     3241 agcgggcgta gctgtttgtg ctccacagct tccagtaatt gcttttgcaa tttcattacg
     3301 cctcctccag ggtcataccg gcaaacatct catccatcag ggcaatcaac ccggcgttgg
     3361 gtcgggttgt gtaaatccct tgttgcggat gttctttatc aacgccacgc aggaacagat
     3421 agataacgcc gccaaagtga agatcatagt cgtaatcagc aatgcgatgg cgcagataac
     3481 gatgcagcgc cagggtataa agctgatatt gcagatcata gcggtgtgcc tgcatcgctg
     3541 ctgccatagc ctgttgggtg taagccgaac tgtcttcacc cagccagttg gatttatagt
     3601 cgagcaggta ataacgccct tcgtggcgga ataccaggtc gataaagcct tttaacatgc
     3661 cgcgtacctg catgaactcc agcggcgggc agccagcgga tagcgggtca aactggcgaa
     3721 ttagtgcatc aagctggctg gcgatgagcg gttcgctaat cggcaggtaa aactccatct
     3781 ccacctgttt atcgcggtcg gaaagctgac tcaggctaac acccgtttca ttgagaggtg
     3841 cctggaggac agccgtgatc cactcggtca aaaccggttc ccactgcgat tcaaagccgc
     3901 cgagttccag tttttcctgt acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt
     3961 caaacaaact gtgcaagaat gtccccggtg acgcaccgcg cgggaactga tgcggtgtta
     4021 acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc cgtggcatca
     4081 gatcctgagc gataccgtga ccacgctgct gcaagccgga gtagctggtg acacgccagt
     4141 tatcgccggg caatcgttgt agtgtcctcg cattcagttc tgcagttaaa gcatcattaa
     4201 cctgccaggg ctggttatca ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata
     4261 acgcttcaat acaggtgcga agccctgccg catcttgcgg ttcccctttt tgcagcaaac
     4321 gcccgagcgc actttggtgg acgtcggtgt cacctttttt atcgccacga cggcgcacca
     4381 gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac
     4441 gcagatcttc cgccagacgt tcgacctccg cgaggtcgac gctttctggc gcagcattaa
     4501 gatccaaaac tgcctcaaac gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga
     4561 aattggtaat aaatggcaac cagaccagcg gatattccag ccctttcgat ttatggatcg
     4621 tgacaatctg caccagatgt ttatcacttt cgaggcgcat ttgttggctg gaggcattac
     4681 tgtctggctc gaggatatgt tgcgataacc agcgtaccag cgcatgttca ctttccagct
     4741 gcgttccggc ttcttgaagc agttcgctga tatgcaagat atcggtaaga cgccgctcac
     4801 cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca
     4861 tcggcataac gccacgtttg cgccagattt gccgataacc atcgaactct tcgactaccg
     4921 catcccacgc atgttcgtca ttattcagcg tttcaatatc cagcgcattc agccccatca
     4981 ttgacgttgc cagcgcactg cgcagggtgt tctcacgttc gggcgtcatc accgcctgca
     5041 acaaccaaag catttcctgc gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt
     5101 aaacggaagg gatttccagc aacgttaagg catcgcgcac ctgggcggcc tcctggcggc
     5161 tgcgcaccag cacgctgatg tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca
     5221 gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg gatttgcgca gcacatacct
     5281 gcgccatggt actttgatag tcgccaacgc cgcagctttc gccttccatc agccacattt
     5341 tcatcgcagg ctgcgtttca cctttaaata caaaacgtaa cgcctgattt ttcccggctg
     5401 atttcactgg aataaacggt atttcgcgaa acatgaacgt gtcatcagtc tggctgaaaa
     5461 gcttgttcac actgttcacc attccaggtg cggagcgcca gttggtgtct aaagtgtagt
     5521 gggcatgaac ttcactacgc gccttcatat aagtgaagat atccgcaccc cggaatgcat
     5581 atatggcctg cttcgggtcg ccaatcagca acaatgcggt ttccggctga tggtgccaga
     5641 tacggcgaaa aattcggtac tgctgggggt cggtatcctg aaattcatcg atcattgcca
     5701 ccgggaatcg cgtacggatc gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg
     5761 aatcgagccg acttaacatg tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg
     5821 ctactgtttc gcggatctca gccaatgcgc gggtgatcaa cagatcgcgg atcgacaatg
     5881 gttctgcaag cagttgatcg atcgcctcaa acagtggatg tcgcggggtt tcccccccgg
     5941 ccttcgtgcg atcttctaag aaacgctggg agaatttttc cagcgactcc ggcaactgat
     6001 aactgtttgt ctcttcttct gcccaggcgc tgatcttttc gatccattta gcctgattgc
     6061 tacggttaaa cttgcgtcga tcaataccag aagattcgat cagcgcatcc agttcaccca
     6121 ctgcgtcgcg ccactgctgt tttaccgtat caatacgcgc cacaatttgc gcgtggcggg
     6181 aagccagcgt ttcatcatcg ggcggcggtg ctttgataac cggcggttcg ccttgcagat
     6241 aacgattaat atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag acgacctggg
     6301 ctatttcacg cggcagcggg tagcagtggc gacgccagaa atcggcgcag gcctggtagc
     6361 gtagcagaga ctcatcttca atcagctgct gctcaaacag catgccggat tcaaaggcat
     6421 tcaggttgag catgcgctgg caaaagccgt gaatagtaaa gactgccgct tcatccatct
     6481 gccgttcggc taacaacaac cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca
     6541 ggcgttcgta cagtggattg tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga
     6601 tattgctacg gatacgaccg cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca
     6661 gcagttcttc aacgatcagc gggcggggaa aggcggcgga accgcctagt ccaagtaaca
     6721 ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt gccggcagag gcttcaatca
     6781 ggcgctcgcc ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca tcactcattc
     6841 ttttcactca tcaggggcat tgtttgctgc aacgcgctga cgttctccca cactttccag
     6901 ccttcaggat gcacatattc ggctttcccg ttctggctgc cggaaatctg cgacagaata
     6961 gccatgcctt gcggctcgac caccgcctga tggaagaaat cagcaagttt ttgcggcgtc
     7021 agcagtttta tctgggccac gattttatca cgcgaatcga agcgcatatt gccgcgatcg
     7081 aaatctttac ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg
     7141 gtaattaccg cctgctggat ttgcgcaaac tcatctggct tcatcgctcg caattttgcc
     7201 tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg tttatcattg
     7261 ctttgcaaaa ggaagcccat gccccactga cgccccacgc tcattggaaa cgcaaacacg
     7321 gcatagccca attgttcttc ggtacgcaac tgattgtaga accacggctg tacgatctgc
     7381 cccaacagag agctataggc tgagctggtg tattcatcgt agccagtcgg tacaaatacc
     7441 gctgccagtg cggagtcggt gctgttaccg gctttttcaa agatgacgga ttgtttttta
     7501 tcgaccacta catctttgtt tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc
     7561 acatcgcgtg ccagcgttgt tgcctgggcc tcggtcatgt tgccgataac cataaactct
     7621 ggtcgagccc ctgattttaa ggcgtcgcga taggccagca cctctttcaa cgtaatggag
     7681 ggcaaaattt tacgccgttc atctcgcgag aagtacggca cttgcgagag catctgcgcg
     7741 ggcataatcg cctgctcaaa cgctttaccc ttttctgcgg aatccatcat ctggttatac
     7801 caggacttcg cctgctcaag ctgctcttcc gtagcggtat agctaaagta cccctcgagc
     7861 aatgcctgga acagctgcgg cagacgctgg gtgtaaccat tagcattaat cataaggccg
     7921 ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg cctggttgct taactgatca
     7981 agcgccagcc ctgcgagata atcattgagc gcaaacatca cctgattgcg ggcgctgtcc
     8041 atggctttcg gattacgcaa aatcaggctg acatcagctt tgggctcgct ggcaaaataa
     8101 cggcttggcg catacaccac gcgcagattc gactcatcaa caatcagctc tggatggtcg
     8161 tatttcttct ctgacttaat cagcgagaaa tcatcaggaa tataagggtt aagctctggc
     8221 aaagagagcg caatgtcggc ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt
     8281 ttatcgacct gatacggcgc atcgacaaag taagccgttt tgttgtgcgg ctctttcggg
     8341 ctgatatacc agatacgcgc attctgcggc gtcatcatcg ccagacgttc ctttactgct
     8401 ttagcatcgt accgatcggc aatattgact gcatccagcg tatgctcaac aggaacgcga
     8461 atcatggtat ctgccagcca ttcgacgtaa tccatatcac gggtgatcga cggataacgg
     8521 aagtcgatat ccagcacatt cgccagttca tcgaagtatt gtttatcaat gcctttttca
     8581 cgtaacagat tgagatagct aaaaattgcc gccacaacct gatcgcgatt agccaggcct
     8641 ttatcagtta aagacgcaga gatcgctaat acgccgctgt tgccgttgac gataggatcg
     8701 gagttggcgc taatgccctc aactaatccc tgcttttgca gccagtcaga aagtgtacct
     8761 gggctgcgat tgccaatcag ataggtaatc aattcatcgg ttttactacg gaacttcgct
     8821 gagttgttat cgatgcgaaa ctcaacgcgc aacactttac gcggcagcgc agggacgtaa
     8881 tgaatgataa tgcccttttg cgcgtcggtg actaccggca cggtgatttc cggttttttg
     8941 ctctctttgt tcggcacgcg accaaaggtg tccgccgcca tttttgccaa ctccggcagc
     9001 ggtttattac tgtaaataac cgccttcatc aaattggcgg aatagtactt ctcgtggaaa
     9061 tctttcagcg cctgctgcac cggattacca ggtttgtcgc ttaaagtttc gaggttacca
     9121 ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg
     9181 cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt tcactgcatt acgctcacgt
     9241 tcggcgtatt tcttgtcgag caagggttct gcaatagcat cggccaggcg gtctaccgca
     9301 ccgggcaagg cgtcgttctc aacttccaga tagaaagccg tgcgatacgg cgcagtgctg
     9361 gcattgtgac taccgccgtg cattttgaga tattcggcca gactgtcagc ctgcgggtac
     9421 tttttcgacc ccatcagact catatgttca aggtaatgtg ccagcccctg gtacgcctcg
     9481 ggatcttcca gcgacccaac gggcactacc agcgccgaga gcgatttaac tgcctgcgga
     9541 tcagaaacca gcaagaccac cataccgtta tccagacgta tagcctgata ctggcggtta
     9601 tctttatcac ttttacggat ggtttcctga atcggctgcc atcccgtttc tgcctgactt
     9661 aatggtgccc agagggcaac aaacaacaat aatgctttga accaggtgct gcggggcatt
     9721 cacggacctc ataatcaact ttattttctg gacagattca acaggttaac gcaaatcatc
     9781 tgccagaatt taatcttgtg ctgcacgagt cagcctatgt ttatataacc atcagtccgt
     9841 gactggtgcg catcataaag taagcagata gattgcgcaa tttttataca acactcatga
     9901 ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga tggcctccat
     9961 tgtctctggt gttaattgcc gccagagccg ttgataccag atatcatcac cttcgccacg
    10021 caccatcatg ttgccttcgt aagcctgaag gaatttcgta cgggcttttt gcaacgtgga
    10081 atcgtcatcc agcatggcat cgttttgcgc gtcataacag gtttttagcc acgcgccgcc
    10141 actttcaggt aacaccagca atggcgcgga cattccttca cgatacccct caatcagttg
    10201 tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga aaacgccact caccgtcttt
    10261 gcgtagaaaa aggcgacttt cgccattacc accgctggca cagtagacaa ggtgttccag
    10321 ccaaagttgc attccctgcg ccacacttaa taaagaggga cgccagcgca acaggccatc
    10381 cggctgcacc tgcggcaacc agccagttat ctgcacaccg ttgcaggcga gatcaatttc
    10441 catactctgc cccggctggc gacaggcaat gactctgtcg gcaagctgct gcatctcctg
    10501 gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg
    10561 gaagcggcgg aacaagcgtt cggcatcatc ctgctcaacc agtgcattca ataactgctg
    10621 attgatttga taacggctaa gtccttccag aataaatggc tcggtgtctg ggatttcgct
    10681 gtcttcagta tggaagttca cctgcaaacg catctggaag aacgcccgca ccggatgtgc
    10741 ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg gcagcggctg
    10801 aacaaattca gaatgtgctt taccagcctg gctggccgca ggtagccatt cacgagcata
    10861 gctttgtcgt tcgcctggct ggtagttttg cggatcaaac ggcatccggg tatggaggca
    10921 agtaagatgc gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg
    10981 tagataatga ctttgcccga tgtagtcgat cagttcctgc accagtaccg acgggaaacg
    11041 ttcactgtta tcctgaatgg aacgcccgat atagctgata tagagttttt gctgcgcgga
    11101 aattaacgct tccaggaaca ggtagcggtc gtcatcgcga cggctacggt cgccacgctt
    11161 cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc
    11221 gttcattccc agcaggcaaa ccactttgaa cggaattgaa cgcattggca tcagagtaca
    11281 aatgttaacc ggtccggcga gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc
    11341 cagttcatca cgcaatagtg acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc
    11401 ggcgataatc gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc
    11461 cggcaggaag aaggcgttga gcatatcgcg acaaaccggc aaccactctt ctagcggacg
    11521 ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt agcagtgaag ccagatgccc
    11581 caccagttct gcaattaagc cgctcgattc atcataaggt agaaccgatt gccactcgcc
    11641 ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc aggccaaatc gccaggtgtg
    11701 ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat
    11761 gccggattcg ttgacccact ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg
    11821 cgccgccagc accggcacat ccagcagcgc cagcacatcc tctgacacaa agcgactgtc
    11881 aggcagtgat aacaggctga taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg
    11941 gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat
    12001 aaacggactg tagctgtcga tatcagccac catcacgatg atgtcgcgcg gagtaagtgt
    12061 cggggcttcc tccagcatcg ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg
    12121 gctatggcaa acgtggaatg tgatactgct atccagtgga tcaagcgggc gtttgttatc
    12181 gctacgggaa aactcttcga tgttcacacc cgcaacggcg cggttttcca gttccagaat
    12241 gtcagactga atgttatgca gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc
    12301 ctggctgctc tccaggtcag aaaggagata aatgtagtcg cgcccaagct tcccccatga
    12361 ggccagcagc gagttgccga catcctgttc accatcgctg ttaaagagct gtccggcatt
    12421 ttcgctatcg cgaaatagcg gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt
    12481 cagcagtttc gccagataag caggatcttt aatatcgccc cagtaataac ggcaggggtt
    12541 ggtaaacagg agatggattt caatatgttt acccagcgcc tggagcgcct ggagataaac
    12601 aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt
    12661 cgtcgcggac tccagcgttt cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg
    12721 ttgtccgagt tcatgggtat attccaccag cgccttccac aacggggctt gccaggcctg
    12781 tgcttctccc agcccttcaa ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg
    12841 atagaccaga tactggtcaa acaggtccgc cgcttttgag gaaagctgga acagttttcg
    12901 cttgtcgcta tcgtcagtca gataatgccg caacagggta aagtcttcgc gctccagcaa
    12961 ttgcggcagc agagtcatca gtttccagct catgctctgt ttgttaaagg cgctctcttt
    13021 ggggatttcc ggtaacaccc ggacgaacat atcccagata aagctcgctg gcagcggaaa
    13081 atcaatgttt gccgcaatac caaacttttg cgacagggtc atttgcagcc actgcgccat
    13141 accggtactt tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc
    13201 gacaataaac tccatcaacg cttccagcac gtccagacga ttggaatggt agacccttaa
    13261 catagcggct cctgactact gacaattcgg gcaatgcagg cgggtcatct cgccttctct
    13321 gcccccgggt gaaactagcg taacgctgat gctgacacat cccgcctgcg atgtctgcat
    13381 tcggttgacc tgccagttgg caggtggcga aatcgcgcgc agttgcgttt gctgccaacc
    13441 atgccgccag agcttttggt actggtttct gctggcgaga ctgttcattg atgttcgctg
    13501 gatacccgat aacgcagtga caatcatcac catcagcacc atcagcacca tcagcaccat
    13561 cagcaccatc gcaaccatta cctccggcag gctaaagcct tgttgattct tcagggaagc
    13621 tgacataatg ccccctcttt cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca
    13681 atttttccat caatgacttc gcctgttcgc cacaacgaaa cgccttcata accggcaatc
    13741 aataaggctt cattatctgc cagtaaacgc aaacaaacct gggcatcggt tccagcgtac
    13801 tgcgagcact gaactgctgg ctgcgtctgc caggagtgca tttttcccca cgccagcgcc
    13861 gactgaacga tggcctggcg gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa
    13921 ctgcgatcct gctgactcat tccttgcaat agcaagctac ccaaaaccag cagcatcagg
    13981 accagagcca gtgacgaaac acctttttcg cggttcacag gttgaatcct gtcacgctat
    14041 agctggcatc caccacggtt tgcggttcag acttactggc agcacgcata ttaaccgtca
    14101 acaccggcga gaagccgctg acatcctgac gtacgacctg aaaagtgtcg ataatgatgg
    14161 catccggatt agtgacttta tcccagccct taccttcaca ggatgtcgca ccgcgtagcg
    14221 tttccagcac atgctccttc agacgaaatc caatctggtc ggactctttt accggttcgc
    14281 gatcccagat accgttactg ttcgcatccc actgcacaat gatacagtca ccctgtccga
    14341 caatttccag cccttcgccg gtacagctgc catgacaata acccgccctc tggagatgct
    14401 tcgcgacggt aaatacccgc agccagattt catcttccag tgccagctta cgagtgctcg
    14461 ttaaactttc acgctgtaac gcaggcagaa agcgtgcagc ccccagcaac aatacgctac
    14521 tgatcgccat agcaatcaac acttccagca gagaaaaacc ttgctctttt acaggcatcc
    14581 ttctgtttct ccttgctgat aaagccggag tcttccccac ggcgaaacca ccagccacca
    14641 ctcgcccgtt gagtttttga agcgaatatg cacggcccat gcggtattgc gcaggccaaa
    14701 gaaagcaagc gaaggtgtca ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac
    14761 caatggtgaa ctgccatgac aggtattggc cccagcagcg gaactcacaa ggcaccataa
    14821 cgtcccctcc ctgataacgc tgatactgtg gtcgcggtta tgccagttgg catcttcacg
    14881 taaatagagc aaatagtccc gcgcctagct ggcggtttgc caaagccgtt gcgactgctg
    14941 ccagtattgc cagccataga gtccacttgc gcttagcatg accaaaatca gcatcgcgac
    15001 cagcgtttca atcagcgtat aaccacgttg tgttttcatg ccggcagtat ggagcgagga
    15061 gaaaaaaaga cgagggccag tttctatttc ttcggcgcat cttccggact atttacgccg
    15121 ttgcaggacg ttgcaaaatt tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa
    15181 accgacgcac actggcgtcg gctctggcag gatgtttcgt aattagatag ccaccggcgc
    15241 tttaacgccc ggatgtggat cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa
    15301 gatggattcg ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa
    15361 ttgcagatga gtttgatcca tatggttgct gtacaggtgc gtatcgccac cggtccagac
    15421 aaaatcaccc acttccagat cgcactgctg cgccatcata tgcaccagta acgcgtagct
    15481 ggcaatgttg aacggcaggc cgaggaagac gtcacaggag cgctgataaa gctggcaaga
    15541 gagtttgccg tctgccacat agaactggaa gaatgcatgg cacggtgcca gcgccatttt
    15601 atccagttcg cctacgttcc acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt
    15661 cagctggttc agtaccgtag taatctggtc aatatgacga ccatctggcg ttggccaggc
    15721 gcgccactgt ttaccataca ctggcccgag gtcgccattt tcatcggccc attcgtccca
    15781 gatggtgaca ttgttttcgt gtagataagc aatgttagta tcgccctgca ggaaccacag
    15841 cagttcgtgg atgatggaac gcaggtggca acgtttggtt gtcaccagcg ggaatccatc
    15901 ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc gttccggttc cggtacggtc
    15961 gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt tctaaatact gtttcatggt
    16021 tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc cagaccatca tgatcacacc
    16081 cgcgacaatc atcgggatgg aaagaatttg ccccatgctg atgtactgca cccaggcacc
    16141 ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc
    16201 aatcaggaac aagcctgaga cagctcccat tgggcgcggt ttacgaatat acaggttgag
    16261 gataataaac agcaccacac cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg
    16321 cagcacaccg taagtgtcga aaatggattg ccactgcggg ttggtttgca gcagcaaaat
    16381 atcttctgta cgggagcctg ggaacagcat ggcaaacggg aagttcgggt caacgcggcc
    16441 ccacaattca ccgttaataa agttgcccag acgcccggca ccaagaccaa acggaatgag
    16501 tggtgcgata aaatcagaga cctggaagaa ggaacgttta gtacggcggg cgaagataat
    16561 catcaccacg ataacgccaa tcaggccgcc gtggaaagac atgccgccgt cccagacacg
    16621 gaacagatac agcggatcgg ccataaactg cgggaaattg tagaacagaa cataaccaat
    16681 acgtcccccg aggaagacgc cgaggaagcc cgcatagagt aagttttcaa cttcattttt
    16741 ggtccagcca ctgcccggac gattcgcccg acgtgttgcc agccacattg caaaaatgaa
    16801 ccccaccaga tacatcaggc cgtaccagtg aagcgccacg ggtcctattg agaaaatgac
    16861 cggatcaaac tccggaaaat gcagatagct actggtcatc tgtcaccaca agttcttgtt
    16921 atttcgctga aagagaacag cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg
    16981 tgcgaataat agcacaaggg gacctggctg gttgccggat accgttaaaa gatatgtata
    17041 tgatccgcgc tataaccctc cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc
    17101 tgcaacctga tgacgaactt cggtcgccag ttgcgcttcc agactacgct gcgcaagatt
    17161 ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt acccgcgcta cagaacgtcc
    17221 gttcatagac agatggcgat acccaagccc aatgaggatt gccacgcaca tgggatcgcc
    17281 cgccatttca ccgcacaaac gaagatcgat accatgtatt tccgcttccc gggcgatcat
    17341 cgccagagct cgtaacattg caggatgaag actgtcgtaa atgttcgcca cccgggtatt
    17401 gttgcgatca acggccagaa tgtattgagt cagatcgttg gtgccaacag agatgaaatc
    17461 gaccctcttt gccagatgcg gcagcataaa taccattgac ggcacttcca gcatgatgcc
    17521 gatacgtggt ttgggaattt cgtaaccgat catctcctcg acttcacgtc cggcacgttc
    17581 aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg accatcggca acagaatatt
    17641 caggttgccc gtagcggcat tagcacgcag catcgcccgc acctggatca agaagatctc
    17701 cggctgatcg agcgtaatgc gaatcccacg ccaacccagg catggattct cttcgctgat
    17761 cggcatgtaa ggcagctgct tatctgctcc gacatccagc gtacgcaagg tgacgggttt
    17821 atcattgaac atttgcagca tcccctgata ctgcgccacc tgttcttctt ccgacgggaa
    17881 accactttgc agcatgaatg ggatttcagt gcgataaaga ccgatgccat caatacagct
    17941 gcccagtttt tcttcatgtt ccgggcttaa accagcattg agcatgactt ttatacgctc
    18001 accgcttttt aactgcgcgg gtaaattgac gtcatcttcc gccagacggc taagctcaat
    18061 ctcttcacta attagccgct gatattcttg cagcagtacc ggctccggat cgaccagcaa
    18121 ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc agcaccgaag gctgaatatc
    18181 cgcgcccatc acggtaggga tccccagcgc acgtaccatg atcgcagcat gggagttggc
    18241 tgcgccatct cgcacgacaa caccgactaa gcgatcctgg ggcagctcag caagagttgt
    18301 cgctgacagt tcatctgcca ccagaatgaa acgttccggc caggcgttcg gcccttgatt
    18361 agcgtcatca agatgaaaca gcaatcgctg acccagcgca cgtaaatcac cagcccgctc
    18421 tttgagatag ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt
    18481 taccgcccac tctgccaccg agcctttatc aacctcggca aacaattcgc gacgcagccg
    18541 ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc
    18601 ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt
    18661 cagtcgttcg cgttccagag ccggatccag cgttgatgcc tgatacacct gttccattaa
    18721 aggtaacgtg gcatcctgcc agccttcggc aatcgccaca ccaggtgctg ccggtaatgc
    18781 gcggattcgc gtctggcgat attgcccaaa cagggcagtc aactgcgact gagaaagaat
    18841 agctgccatc tgggtggcaa gcgtcaccag gaaggattct tcactttcgt catactggcg
    18901 caactctcgt tgctgtacca ccagtacacc aagcaactgg cgacgttgaa taattggtac
    18961 gcctaaaaac gcgcggaaac gttcttcttt tacggagggg atgtatttga agctggggtg
    19021 cttttgcgca tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc cgacgatccc
    19081 ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat
    19141 caggtagtaa caacgtcgat catgatcggc caggtagacc gaacagacct cggtatccat
    19201 cgcaagacag atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc
    19261 taccttttcg actatttcgc gcaggcgagt gagcataatt ggcgtgactt aacctctttt
    19321 acgtcgataa gcagatgcgt tttgtggttt tggcgtattt tcctgcagtg acatcaccac
    19381 actcgcgaac tctttcatta ccctacggta gacatcacgt ttaaatgaca ccacctgtct
    19441 gaccggatac cagtaactta cccatcgcca gccgtcaaac tctggtgtac tgctggtttg
    19501 catattgatt tctgcatcgc cgcttaccag ctgcaagaga aaccattttt gtttttggcc
    19561 gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt agcgcaacca
    19621 gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa
    19681 ttcacggtac atcgcctgct ctgcggattc tccggggttg attccgcctt gcggaaattg
    19741 ccaggagtgc tgaccaaatc gccgggccca cattacctgc ccctggcgat tacaaatcac
    19801 gatacctacg tttgggcggt agccatcgtc atcaatcacc ggactacctc aaaataaagc
    19861 tttatatacg aatgattgtt tcatactcca ggaagacggt aaaccactct ctgcagggca
    19921 ttacacacta ataacaattg aataactcac agttatgtgc agagttataa acagaggaag
    19981 gggtggatag ccgtttttat tcaccttttc tgtggataga gttgtgaaga actacggaat
    20041 tactacggga aaacccggag cattctgaat aagcccgata ta
//
LOCUS       DAZPEU010000063        19698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPEU010000063 DAZPEU010000000
VERSION     DAZPEU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.063"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHU2L_002015"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHU2L_002015"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR1356877.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHU2L_002016"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHU2L_002016"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR1356878.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHU2L_002017"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHU2L_002017"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR1356879.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHU2L_002018"
     CDS             6038..6658
                     /locus_tag="ACHU2L_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR1356880.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHU2L_002019"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHU2L_002019"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR1356881.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHU2L_002020"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHU2L_002020"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1356882.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHU2L_002021"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHU2L_002021"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR1356883.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACHU2L_002022"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACHU2L_002022"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR1356884.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACHU2L_002023"
     CDS             complement(10068..11069)
                     /locus_tag="ACHU2L_002023"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1356885.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHU2L_002024"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHU2L_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHU2L_002025"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHU2L_002025"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR1356886.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHU2L_002026"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHU2L_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR1356887.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHU2L_002027"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHU2L_002027"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR1356888.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHU2L_002028"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHU2L_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR1356889.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHU2L_002029"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHU2L_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1356890.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHU2L_002030"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHU2L_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR1356891.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4684 a         5506 c         5030 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcactcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAZPEU010000064        18835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPEU010000064 DAZPEU010000000
VERSION     DAZPEU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.064"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHU2L_002031"
     CDS             <1..122
                     /locus_tag="ACHU2L_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR1356892.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHU2L_002032"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHU2L_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR1356893.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHU2L_002033"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHU2L_002033"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR1356894.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHU2L_002034"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHU2L_002034"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR1356895.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHU2L_002035"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHU2L_002035"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR1356896.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHU2L_002036"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHU2L_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR1356897.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHU2L_002037"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHU2L_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR1356898.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHU2L_002038"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHU2L_002038"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR1356899.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHU2L_002039"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHU2L_002039"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR1356900.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHU2L_002040"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHU2L_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR1356901.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHU2L_002041"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHU2L_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR1356902.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHU2L_002042"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHU2L_002042"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR1356903.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHU2L_002043"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHU2L_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR1356904.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACHU2L_002044"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACHU2L_002044"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR1356905.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHU2L_002045"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHU2L_002045"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1356906.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHU2L_002046"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHU2L_002046"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR1356907.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..>18835
                     /gene="rfbA"
                     /locus_tag="ACHU2L_002047"
     CDS             18000..>18835
                     /gene="rfbA"
                     /locus_tag="ACHU2L_002047"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1356908.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KK"
BASE COUNT         4655 a         4613 c         5149 g         4418 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaa
//
LOCUS       DAZPEU010000065        18224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPEU010000065 DAZPEU010000000
VERSION     DAZPEU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.065"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHU2L_002048"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHU2L_002048"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR1356909.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHU2L_002049"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHU2L_002049"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR1356910.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..3000)
                     /locus_tag="ACHU2L_002050"
                     /pseudo
     CDS             complement(1728..3000)
                     /locus_tag="ACHU2L_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3183..4388)
                     /locus_tag="ACHU2L_002051"
     CDS             complement(3183..4388)
                     /locus_tag="ACHU2L_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1356911.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="ACHU2L_002052"
     CDS             complement(4488..4844)
                     /locus_tag="ACHU2L_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR1356912.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="ACHU2L_002053"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHU2L_002053"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHU2L_002054"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHU2L_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR1356913.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="ACHU2L_002055"
     CDS             6774..9830
                     /locus_tag="ACHU2L_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR1356914.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="ACHU2L_002056"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="ACHU2L_002056"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR1356915.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="ACHU2L_002057"
     CDS             10337..10525
                     /locus_tag="ACHU2L_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR1356916.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="ACHU2L_002058"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="ACHU2L_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR1356917.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="ACHU2L_002059"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="ACHU2L_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR1356918.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="ACHU2L_002060"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="ACHU2L_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR1356919.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="ACHU2L_002061"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="ACHU2L_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR1356920.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="ACHU2L_002062"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="ACHU2L_002062"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR1356921.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHPLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="ACHU2L_002063"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="ACHU2L_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR1356922.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHU2L_002064"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHU2L_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR1356923.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHU2L_002065"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHU2L_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR1356924.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4790 a         4406 c         4681 g         4347 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 cgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc atcccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catc
//
LOCUS       DAZPEU010000066        18128 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPEU010000066 DAZPEU010000000
VERSION     DAZPEU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.066"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..793
                     /locus_tag="ACHU2L_002066"
                     /pseudo
     CDS             1..793
                     /locus_tag="ACHU2L_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACHU2L_002067"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACHU2L_002067"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR1356925.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACHU2L_002068"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACHU2L_002068"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR1356926.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACHU2L_002069"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACHU2L_002069"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR1356927.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACHU2L_002070"
     CDS             complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACHU2L_002070"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR1356928.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACHU2L_002071"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACHU2L_002071"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR1356929.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="ACHU2L_002072"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="ACHU2L_002072"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR1356930.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACHU2L_002073"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACHU2L_002073"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR1356931.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="ACHU2L_002074"
     CDS             complement(12980..14320)
                     /locus_tag="ACHU2L_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR1356932.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACHU2L_002075"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACHU2L_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR1356933.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="ACHU2L_002076"
     CDS             complement(16108..16557)
                     /locus_tag="ACHU2L_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR1356934.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACHU2L_002077"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACHU2L_002077"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR1356935.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="ACHU2L_002078"
     CDS             complement(17357..17686)
                     /locus_tag="ACHU2L_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR1356936.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(17933..>18128)
                     /locus_tag="ACHU2L_002079"
     CDS             complement(17933..>18128)
                     /locus_tag="ACHU2L_002079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356937.1"
                     /translation="RLREKDDSIFVDRESDNEVNIRMMTLLQDTPGWCFRERLLDEAK
                     RMTQDALKDTSRMENENRSG"
BASE COUNT         4444 a         4909 c         4314 g         4461 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctagt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccaaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16861 cgtcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtctt
//
LOCUS       DAZPEU010000067        17918 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPEU010000067 DAZPEU010000000
VERSION     DAZPEU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.067"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHU2L_002080"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHU2L_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR1356938.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACHU2L_002081"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACHU2L_002081"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR1356939.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACHU2L_002082"
     CDS             2826..3980
                     /locus_tag="ACHU2L_002082"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR1356940.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHU2L_002083"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHU2L_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR1356941.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACHU2L_002084"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACHU2L_002084"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR1356942.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACHU2L_002085"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACHU2L_002085"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR1356943.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHU2L_002086"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHU2L_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR1356944.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACHU2L_002087"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACHU2L_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR1356945.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACHU2L_002088"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACHU2L_002088"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR1356946.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACHU2L_002089"
     CDS             11296..11511
                     /locus_tag="ACHU2L_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1356947.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHU2L_002090"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHU2L_002090"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR1356948.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACHU2L_002091"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACHU2L_002091"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR1356949.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACHU2L_002092"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACHU2L_002092"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR1356950.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACHU2L_002093"
     CDS             complement(16247..16786)
                     /locus_tag="ACHU2L_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR1356951.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACHU2L_002094"
     CDS             complement(16802..17320)
                     /locus_tag="ACHU2L_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1356952.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="ACHU2L_002095"
     CDS             complement(17631..17822)
                     /locus_tag="ACHU2L_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR1356953.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..>17918
                     /locus_tag="ACHU2L_002096"
     CDS             17840..>17918
                     /locus_tag="ACHU2L_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356954.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKA"
BASE COUNT         4212 a         4412 c         4948 g         4346 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgt
//
LOCUS       DAZPEU010000068        17908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPEU010000068 DAZPEU010000000
VERSION     DAZPEU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.068"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="ACHU2L_002097"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="ACHU2L_002097"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR1356955.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="ACHU2L_002098"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="ACHU2L_002098"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR1356956.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /gene="yjgA"
                     /locus_tag="ACHU2L_002099"
     CDS             complement(2650..3201)
                     /gene="yjgA"
                     /locus_tag="ACHU2L_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR1356957.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="ACHU2L_002100"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="ACHU2L_002100"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR1356958.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5212
                     /gene="cybC"
                     /locus_tag="ACHU2L_002101"
                     /pseudo
     CDS             4830..5212
                     /gene="cybC"
                     /locus_tag="ACHU2L_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="ACHU2L_002102"
     CDS             complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="ACHU2L_002102"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR1356959.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="ACHU2L_002103"
     CDS             complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="ACHU2L_002103"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR1356960.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8411..10066)
                     /gene="treC"
                     /locus_tag="ACHU2L_002104"
     CDS             complement(8411..10066)
                     /gene="treC"
                     /locus_tag="ACHU2L_002104"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR1356961.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10116..11537)
                     /gene="treB"
                     /locus_tag="ACHU2L_002105"
     CDS             complement(10116..11537)
                     /gene="treB"
                     /locus_tag="ACHU2L_002105"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR1356962.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11656..12603)
                     /gene="treR"
                     /locus_tag="ACHU2L_002106"
     CDS             complement(11656..12603)
                     /gene="treR"
                     /locus_tag="ACHU2L_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR1356963.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12788..12841
                     /gene="mgtL"
                     /locus_tag="ACHU2L_002107"
     CDS             12788..12841
                     /gene="mgtL"
                     /locus_tag="ACHU2L_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR1356964.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12982..15678
                     /gene="mgtA"
                     /locus_tag="ACHU2L_002108"
     CDS             12982..15678
                     /gene="mgtA"
                     /locus_tag="ACHU2L_002108"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR1356965.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFRSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="ACHU2L_002109"
     CDS             complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="ACHU2L_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR1356966.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="ACHU2L_002110"
     CDS             complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="ACHU2L_002110"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR1356967.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="ACHU2L_002111"
     CDS             complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="ACHU2L_002111"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR1356968.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="ACHU2L_002112"
     CDS             complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="ACHU2L_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR1356969.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
BASE COUNT         4184 a         4551 c         4812 g         4361 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgctgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaaaagaag cgcaggctgc
     5161 tgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattcctcat
     5221 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
     5281 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
     5341 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
     5401 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttttaccag
     5461 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatccc
     5521 ctggcgtttg atacgagtgt cattcagatc tttaatgatc ttgtcttcca ttgctttggt
     5581 aaatggctga ccagaattta cccgccatgt gcttttgtta tagcaaccgg ggcattcatg
     5641 aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga tgtcgacagg
     5701 atagtactga tgataattca ttatttcgcc tggcttatca gtccgcgtgt aggctgcatc
     5761 cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacagc cgttgccaaa
     5821 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttagcgcag agtgaagatt
     5881 aacctatctg cccattcccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
     5941 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
     6001 cacgggaggc gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg gtgcactcga
     6061 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
     6121 gtacatgctg atagctgtaa tcccagacat cttccagcgc tttcaggttg tgctgaatgt
     6181 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
     6241 agtcgatctt gtcgtacggg ttcaccttct tctccacatc aaggtggaaa ctgttggtgt
     6301 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
     6361 agcggtcgca caggttttca ctcggcgtgc tgtaaagact gaaaccataa cccgtttctt
     6421 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgaa
     6481 gcttctcgtt gtcgtaaaca tgctcgccgc tgaacagcgc gttaatggtt tcgtggatgc
     6541 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acatcatcgt
     6601 cagcattcag acgtacgcca caagcacctt ccatatagag gatcggggcg acgcgcgctt
     6661 tcacgccttc gagacgagcg atacgggtca tcagtgcctt acgtgccagc accagacgtt
     6721 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
     6781 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
     6841 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgacgt
     6901 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
     6961 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
     7021 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
     7081 tacgcaggat cgattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
     7141 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
     7201 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
     7261 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggta acaaacggcg
     7321 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
     7381 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
     7441 cgttccccat tttaaaaccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
     7501 acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
     7561 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
     7621 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
     7681 acgaggcgtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
     7741 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
     7801 gacccgacat cagctggttt tcaactgcgg tctggatctc gttgatatcc acctggttgc
     7861 gcccctgcat ctgctcgttg acaaccgcgg caacagtggc gcaataatcg gcatcatcga
     7921 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
     7981 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
     8041 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
     8101 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
     8161 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
     8221 agtgctttgc atcaaggatg ttttaacttt ttttgatgta gctcaaagta aaaagcagag
     8281 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
     8341 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat tatcggggct
     8401 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa ttcatggcac
     8461 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
     8521 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
     8581 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
     8641 tgccccatgt caggatggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
     8701 acacagagga gtcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccta
     8761 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
     8821 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
     8881 gaccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
     8941 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
     9001 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
     9061 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
     9121 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
     9181 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
     9241 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
     9301 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
     9361 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
     9421 ccaggtcttc agggaaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
     9481 cgtcgacccc acgatcggcc cagaattcac agactttttt cagctctgcg cgtaccgctg
     9541 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
     9601 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
     9661 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
     9721 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
     9781 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
     9841 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgg ggagagacat
     9901 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
     9961 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    10021 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    10081 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    10141 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    10201 attgccagcg caaatacctg ccagtagctc ggttgaatag agagaattcc cggcaggccc
    10261 cctacgccga taccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    10321 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    10381 ggctcagtga ccccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    10441 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    10501 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    10561 agggtggtct ggtgtacgcc ggtgatcacc agcggcgcgt acaggaagcc aaacaatgcc
    10621 gcaccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    10681 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    10741 gaacagacgg gcaccaccac cagatagagg taatccggta cgatgcgttt aaggcgagtt
    10801 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    10861 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    10921 taagcgttca tcagctgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    10981 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaaaatc
    11041 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    11101 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    11161 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac
    11221 tgctcatgcc atttcatatt ttgccgggcg gcttttttta cctgctcttt atcaacctgc
    11281 gcctgtccgg ttgacgctat tagtgcttga tagtaatcac ccacgttggt gccaatcacc
    11341 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    11401 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    11461 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    11521 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    11581 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    11641 tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    11701 cccggttacc tgtgcgatca attggcaggc cgcctggcgt ccagcttcgg cgtagccagg
    11761 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac ctacgctcgc
    11821 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gagccagcgt
    11881 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttttcata
    11941 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    12001 gaacgccagg taggcttcgt gacgtcgctt cccggtcgtc acgtcactat gcggcacgcc
    12061 gagataactg atattgcgat gcccctggtc atagagccgt tgcatcagaa tcttgattgc
    12121 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    12181 cgatgactgc cagtgggcta acatttcttc ggttatgccg gtaaaaccga acagcactac
    12241 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    12301 ctgattttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    12361 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    12421 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    12481 tgcttcaaca cgctcgcgcg tgcgctgact cacgccgctt tcgttattca gcacccggga
    12541 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    12601 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    12661 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    12721 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    12781 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    12841 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa tgtccctgct
    12901 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    12961 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggttcat tcgccattta
    13021 ccttcccgtc tggttcatcg cgatccattg cctggcgcgc agcagacagt gaatacggcg
    13081 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    13141 tggaaaacgt tcgacaccca tccggaagga ttaaatcagg cggaagtgga atctgcccgc
    13201 gaacaacatg gtgataataa attacccgcc caacaaccgt cgccgtggtg ggtacattta
    13261 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    13321 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    13381 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    13441 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    13501 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    13561 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    13621 ggtgagtctc tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatccg
    13681 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    13741 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    13801 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgttagtat gctgctgatt
    13861 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggcgac
    13921 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc ggaaatgttg
    13981 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    14041 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    14101 aaaaccggca ccctgacgca ggacaaaatt gtgctggaga atcataccga tatctccggt
    14161 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaaca gtcattacca gaccggactt
    14221 aaaaatctgc ttgataccgc ggtgctcgaa ggtacggatg aagagtcagc gcgttcgctg
    14281 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    14341 gtggtagtgg cagaaaatac cgaacaccat cagctggttt gcaaaggtgc attgcaggaa
    14401 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    14461 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    14521 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    14581 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    14641 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    14701 ttagttgcag cgaaagtgtg ccatgaagtg gggctggatg cgggagaggt ggtcattggt
    14761 agtgatattg aaacgctatc tgacgatgaa ctggcaaatc tcgcacagcg caccacgctg
    14821 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ctttactgaa gcgcgaaggg
    14881 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    14941 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    15001 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    15061 aacatgctga aatacatcaa attgacggcg agttctaact tcggtaatgt gttcagcgtg
    15121 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    15181 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    15241 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    15301 ccgatcagct cgattttcga tattctgacg ttttgcttga tgtggtgggt attccatgcc
    15361 aacacgccgg aaacgcaaac gctgttccgg tcgggctggt ttgtggtggg attactgtcg
    15421 caaacgctga ttgtgcatat gatccgtacc cgccgtgtgc cgtttattca gagctgtgca
    15481 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    15541 tcaccgctgg ccagttatct gcagctgcag gcgctgccgt taagctattt cccgtggctg
    15601 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt
    15661 cgttacggct ggcagtaaag aataaactgg gcatggtggc ccggattcag attgcagaca
    15721 aagtgagctt tgttcatgcc ggatgcggcg taaacgccca atccggccta caaattcaat
    15781 aaattgcagg aatcacgtag gcctgataag cgtagcgcat cgggcgattt tgcttttgtc
    15841 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    15901 ttgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    15961 ccgggaaagt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    16021 agtcgttcag atctttcacg aacacagtgg ttttaacgat gtcgcccact ttcaggccag
    16081 cggcttcgac gattgctttt acattatcca gcgactgacg tgcctgtgca gcgacgtctg
    16141 ccggtacttc accagttttc ggatttaccg ggatctgacc ggaggtgatg atcatattgc
    16201 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    16261 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    16321 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    16381 cacagtattt gcatttaagc gcgatgtcat tggcgcgttt tcgcacggca aagctggatg
    16441 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    16501 gctctggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    16561 tggcttgcgg tgcatacaat gccagttggt ctacttgctc ttcactcaaa aaggtatttt
    16621 cgattttgat cagatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    16681 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    16741 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    16801 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    16861 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    16921 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    16981 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    17041 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    17101 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    17161 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    17221 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    17281 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    17341 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccagaaaatt
    17401 cggtggctag gcgtgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    17461 tgctgataac cgaaatggta tcggccagcg tttcgccttt tttacccagt gatgtattgg
    17521 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    17581 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    17641 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    17701 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    17761 tcctgacacc taggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    17821 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    17881 actgaaccat acatcctccc ggcaaatt
//
LOCUS       DAZPEU010000069        17822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPEU010000069 DAZPEU010000000
VERSION     DAZPEU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.069"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            10..534
                     /locus_tag="ACHU2L_002113"
     CDS             10..534
                     /locus_tag="ACHU2L_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR1356970.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            615..1289
                     /locus_tag="ACHU2L_002114"
     CDS             615..1289
                     /locus_tag="ACHU2L_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1356971.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1305..3767
                     /locus_tag="ACHU2L_002115"
     CDS             1305..3767
                     /locus_tag="ACHU2L_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR1356972.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3783..4816
                     /locus_tag="ACHU2L_002116"
                     /pseudo
     CDS             3783..4816
                     /locus_tag="ACHU2L_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4898..5236)
                     /gene="rcnB"
                     /locus_tag="ACHU2L_002117"
     CDS             complement(4898..5236)
                     /gene="rcnB"
                     /locus_tag="ACHU2L_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR1356973.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5455..6285)
                     /gene="rcnA"
                     /locus_tag="ACHU2L_002118"
                     /pseudo
     CDS             complement(5455..6285)
                     /gene="rcnA"
                     /locus_tag="ACHU2L_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6407..6679
                     /gene="rcnR"
                     /locus_tag="ACHU2L_002119"
     CDS             6407..6679
                     /gene="rcnR"
                     /locus_tag="ACHU2L_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR1356974.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6771..6867
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6902..7690
                     /gene="thiM"
                     /locus_tag="ACHU2L_002120"
     CDS             6902..7690
                     /gene="thiM"
                     /locus_tag="ACHU2L_002120"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR1356975.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7687..8487
                     /gene="thiD"
                     /locus_tag="ACHU2L_002121"
     CDS             7687..8487
                     /gene="thiD"
                     /locus_tag="ACHU2L_002121"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR1356976.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8552..9369
                     /locus_tag="ACHU2L_002122"
                     /pseudo
     CDS             8552..9369
                     /locus_tag="ACHU2L_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9421..10167
                     /locus_tag="ACHU2L_002123"
     CDS             9421..10167
                     /locus_tag="ACHU2L_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1356977.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10141..11106)
                     /locus_tag="ACHU2L_002124"
     CDS             complement(10141..11106)
                     /locus_tag="ACHU2L_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR1356978.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11103..12106)
                     /locus_tag="ACHU2L_002125"
                     /pseudo
     CDS             complement(11103..12106)
                     /locus_tag="ACHU2L_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12146..13198
                     /gene="fbaB"
                     /locus_tag="ACHU2L_002126"
     CDS             12146..13198
                     /gene="fbaB"
                     /locus_tag="ACHU2L_002126"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR1356979.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13506..14360
                     /gene="gatY"
                     /locus_tag="ACHU2L_002127"
     CDS             13506..14360
                     /gene="gatY"
                     /locus_tag="ACHU2L_002127"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR1356980.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14389..15651
                     /gene="gatZ"
                     /locus_tag="ACHU2L_002128"
     CDS             14389..15651
                     /gene="gatZ"
                     /locus_tag="ACHU2L_002128"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR1356981.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15661..16113
                     /gene="gatA"
                     /locus_tag="ACHU2L_002129"
     CDS             15661..16113
                     /gene="gatA"
                     /locus_tag="ACHU2L_002129"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1356982.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16144..16428
                     /gene="gatB"
                     /locus_tag="ACHU2L_002130"
     CDS             16144..16428
                     /gene="gatB"
                     /locus_tag="ACHU2L_002130"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR1356983.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16432..17787
                     /locus_tag="ACHU2L_002131"
     CDS             16432..17787
                     /locus_tag="ACHU2L_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR1356984.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4636 a         4086 c         4544 g         4556 t
ORIGIN      
        1 gaacatatta tgaaacgttc aattattgct gccgctgtct tttcttcttt ttttatgagc
       61 gctggagtat ttgctgcaga cgttgatacc ggaacattaa ccattaaagg aaatattgca
      121 gaatctccgt gtaaattcga agcgggtggt gattcagtaa gtattaatat gccgactgta
      181 ccaaccactg tctttgaagg taaagctaaa tattctacct atgatgatgc agtcggtgta
      241 accagcagca tgttaaaaat tagctgcccg aaagaagttg ctggtgtaaa actctcgttg
      301 atcacaaacg acaaaataac cggtaacgat aaggcgatag ccagtagcaa cgataccgta
      361 ggtgataaca gcgatgtcct agatgtttct gcacctttta acattgagag ttataaaaca
      421 gcggaaggtc aatatgctat tccgtttaaa gcaaaatacc tgaaactgac agataactca
      481 gtgcaatcag gtgatgtgtt atcttctctg gttatgcgtg tggcgcagga ttaataaata
      541 aaattgcaag tttctccagg taataaacat ggccgctatc cccatggcgg ctttttaatt
      601 taagaggcat taagatgaaa ggattattat ctttactcat tttttctatg gtccttcctg
      661 cacatgccgg aattgttatc tacgggacgc gcattattta cccggcagaa aataaagaag
      721 ttatggtgca gttgatgaac cagggaaatc gttcttcgct gctgcaggcg tggattgatg
      781 atggcgatac gtcgttacca ccagaaaaaa ttcaggttcc tttcatgtta acgccaccag
      841 tggcaaaaat aggggcaaat tccgggcagc aagtaaaaat caaaattatg ccgaataaac
      901 tgcccactaa taaagaaagc attttttatc tgaatgttct gtatattcca ccaaatagtc
      961 cagagcaaga aggcaagaat gcactgaagt ttgcgatgca aaacagaatt aagttgtttt
     1021 accggccagc gggtattgct ccggtaaata aagcgacctt taaaaagtta ctggtaaatc
     1081 gcagtggcaa tggattggtg ataaaaaatg actcagctaa ttgggtgacg atttcggatg
     1141 tcaaagctaa taatgttaaa gtcaattatg aaactattat gattgccccc ttagaaagtc
     1201 agagtgttaa tatcaaaagt aataatgcaa ataactggta tctgaccatt atcaatgacc
     1261 acggcaacta tattagtgac aaaatttaat tgcaggagct gcctatgttg agaatgaccc
     1321 cacttgcatc agcaatcgta gcgttattgc tcggcattga agcttatgca gctgaagaaa
     1381 cctttgatac ccattttatg ataggtggaa tgaaagacca gcaggttgca aatattcgtc
     1441 ttgatgataa tcaaccctta ccggggcagt atgacatcga tatttatgtc aataagcaat
     1501 ggcgcgggaa atatgagatt attgttaaag acaacccgca agaaacatgt ttatcaagag
     1561 aagttatcaa gcggttaggc attaatagcg ataacttcgc cagcggtaag caatgtttaa
     1621 catttgagca acttgttcag ggtgggagct atagctggga tatcggggtt tttcgtctcg
     1681 atttcagtgt cccgcaggct tgggtggaag aactggaaag tggctatgtt ccaccggaaa
     1741 actgggagcg gggtattaat gcgttttata cttcttatta tgtgagtcag tattacagcg
     1801 actataaagc gtcgggtaac aacaagagta catatgtacg ttttaacagc gggttaaatt
     1861 tactggagtg gcaactgcat tctgatgcca gtttcagtaa aacaaataac aatccagggg
     1921 tgtggaaaag caataccctg tatctggaac gtggatttgc ccaatttctc ggcacgcttc
     1981 gcgtgggtga tatgtacaca tcaagcgata tttttgattc tgttcgcttc agcggtgtgc
     2041 ggttgtttcg tgatatgcag atgttgccta actcgaaaca aaattttacg ccacgggtgc
     2101 aggggattgc tcagagtaac gcgctggtaa ctattgaaca gaatggtttt gtggtttatc
     2161 agaaagaggt tcctcctggc ccgtttgcga ttacagattt gcagttggcc ggtggtggag
     2221 cagatcttga tgttagcgtg aaagaggcgg acggctcggt aaccacctat ctggtgcctt
     2281 atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa atatgatttt gcggcggggg
     2341 cgagcaagca aagtgatttt gtccaggcag gttatcagta tggttttaat aacttattga
     2401 cgctgtatgg cggcacgatg gttgctaata attactatgc gtttaccctc ggaacgggtt
     2461 ggaacacgcg cattggcgca atttccgtcg atgccacgaa gtcgcatagt aaacaagaca
     2521 acggcgatgt gtttgacggg caaagttatc aaattgccta caacaaattt gtgagccaaa
     2581 cgtcgacgcg ttttggtctg gcggcctggc gttattcgtc gcgtgattac cggacattta
     2641 acgatcacgt ttgggcaaac aataaagata attatcgccg tgatgaaaac gatatctatg
     2701 acattgccga ttattaccag aacgattttg gccgtaaaaa tagcttttct gccaatatga
     2761 gccagtcatt gccagaaggc tggggttctg tgtccttaag tacgttatgg cgagattact
     2821 gggggcgtag cggcagcagt aaggattatc agttgagtta ttccaataac tggcggcgga
     2881 taagctatac cctcgcggca agccaggcgt atggcgagaa tcatcatgaa gagaaacgtt
     2941 ttaatatttt tatatcaatt ccctgtgatt ggggtgatga cgttacgacg cctcgtcggc
     3001 aaatatatat gtctaactca acgacgtttg atgatcaggg ttttgcctca aataatacgg
     3061 gattatcagg aacagtaggg agtcgggatc agttcaatta tggtgtcaac ctgagtcatc
     3121 aacatcaggg aaatgaaacg acagctgggg cgaatttgac ctggaacgcg ccggttgcga
     3181 cagtgaatgg cagttatagt cagtcgagta cttatcgaca gactggagcc agtgtttcag
     3241 ggggcattgt cgcctggtcg ggtggcgtta atctggcgaa ccgtctttcc gaaacgtttg
     3301 ctgtgatgaa tgcgccagga attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa
     3361 caaaccgtaa tggagtggtg gtatacgacg gaatgacacc ttatcgggaa aatcacctga
     3421 tgctggatgt gtcgcaaagc gatagcgaag cagaattacg tggcaaccgg aaaattgccg
     3481 ccccttatcg cggcgcggtt gtactggtta attttgatac cgatcagcgc aagccctggt
     3541 ttataaaagc gttaagagcg gatggtcaac cattaacgtt tggttatgaa gtcaatgata
     3601 tccatggtca taatattggc gttgtcggcc agggaagcca gttatttatt cgcaccaatg
     3661 aaataccgcc atcggttaat gtagcaattg ataagcaaca aggactttca tgcacaatca
     3721 ccttcggtaa agagattgat gaaagtagaa attatatttg ccagtaatag tcaggtggtt
     3781 ctatggaaat tcgcataatg ctatttatat taatgatgat ggttatgcct gtgagctatg
     3841 cggcatgtta tagtgagtta tctgttcagc acaatttggt tgttcagggg aattttgcac
     3901 ttactcaaac acaaatagcg acatatgagc ataattttaa tgattcgtca tgcgtgagta
     3961 caaatactat cacccctatg agcccatcgg atattattgt tggactttat aacgatacca
     4021 ttaaattaaa tttacatttt gaatggacca ataaaaacaa catcacgttg tcaaataatc
     4081 agagcagttt caccagtggt tattcggtaa cggtgacacc tgcggccagt aatgcaaaag
     4141 tgaatatttc tgcggggggc ggtggttcag tgatgattaa tggtgttgcg acattatcca
     4201 gtgcttcatc atcgacacgc gggagtgccg cagtacaatt tctactgtgt ttattaggtg
     4261 gcaagtcatg gggtgcatgt gtaaatagct acagaaatgc attggcacaa aatgcaggtg
     4321 tctattcctt caatctgaca ttgtcataca acccgataac cacaacctgc aaacaggacg
     4381 atttattaat tactttagac agtattcccg ttttacaatt accagccaca ggtaacaaag
     4441 caacaataaa tagtaaaaaa agggatatta ttctgcgttg taaaaattta ttaggtcaac
     4501 aaaatcaaac atcacggaaa atgcaggtgt atttatcaag ttctgacttg ttaaccaaca
     4561 gcaacacaat actgaaaggt gcggaagata atgcgtagga tttattcttg aaagtaatgg
     4621 ttcgccagtc acacttttaa atatcactaa cagcagtaaa ggatacacaa atttaaaaga
     4681 aattgcggca aagtcaaaac ttacagatac aacggtttca attccgataa cagccagtta
     4741 ctacgtctac gatacaaaca aagttaaatc tggcgcactg gaggcaaccg cattaatcaa
     4801 cgtgaaatac gactaataac aagcaaaacg agcaagtggc taataataaa aaataacaag
     4861 gtgtctgcat ttccccctca tgatgagggg gcttttttta gcgatgataa aaaatctcac
     4921 cgtcgtaggc tttaatgatt ttaccgtcgg tgtcgctgat caacacgtac gcgccaccca
     4981 tataggtcca gtgcgtcccg gcatcaggcg cgggcaggtt acgtaactgc cactgcttaa
     5041 tgttgtactc atcggtgcgg tacatctctg ggacggtatc acctggtttg aaatgcttaa
     5101 agtcagcaat gaaactactt aactcatact tagaaattcc cgaggtattg gtcgaacccg
     5161 cggtggctgg tgcggcccag gcggcactgg taaccagcaa aagcacaccc agcaacatct
     5221 tatttttaat ggtcataaac tctccacttt ggggatctgt aatatcagtc gcatatcttt
     5281 cctgtcatac aatataactg acaagcgata ttttgttagt gatcccatga tttcgtaaaa
     5341 agtgtaaatg aaaggatgcc ctaaaagcat ccttagcgag cagagagtca gcggaaatag
     5401 ctgatttaag aatcatcctg ggctgaagat aacatgcaag cctaaaggat tgagttatcg
     5461 cattatgccc atgaagccat gtacgcccat atacacaccg actaagccaa tcaacagact
     5521 ggaaaaatag ggggcgcgtt tagcgagagt gttaaatccg ctccagcgtt ttgcgacctg
     5581 ctgaacgctg attgctgcgc caacgcctac ggtgacaagc gttaacgcca ggccaatgct
     5641 gaaactgacg accagtgttg cgcccagtgt cagggctttc aactgaatgc atatcaacag
     5701 cacggtaatt gctgccgggc aggggataaa gccaccggtt aagccaaata acaaaatttg
     5761 ccagttgatg acctctctac catcaaagcg tcgtttaatg tcattggcat gggctcgtgc
     5821 atgggcatcc tgatactcgc catgttcgtg atggtgatga tgtccttggt cgtggtgatg
     5881 ttcgtgatcg tgatgatggt gatgatgctc ataatcatgc ccgtgcatat tctccagcca
     5941 gttgcgttcg ccgcgctagg tacgccagaa catccagaac gcggtgccaa taatgatcac
     6001 tgcggaaatc agctggagcc acggttctgc tgattgagca gtaaagcgct tactgatcac
     6061 catcccgcca aaggcaatca accagaccac cgcggtatgc gaaatggttg ctgccagtcc
     6121 gagcatcacc gcctgtttaa tggtgccttt gatggcgatg ataaacgccg ccatcatcgt
     6181 ttttgagtgc cctggttcca ggccatgaag cgcaccaagt aagatggcgc tggggatgaa
     6241 gaaccaggcg tttccttgct gaagaagagt tgtaaattcg gtcatgataa taattcttag
     6301 tattaattcg gcaatctgat tctactcccc cccagtacct gatactaccc ccccagtaga
     6361 ttaatagtgc tatgattttt catattcttg ttaaccaggt gttgccatgt ctcatacaat
     6421 ccgtgataaa cagaaactga aagcgcgtgc cagtaagatt cagggccagg tcgtggcgct
     6481 caagaaaatg ctcgacgagc cgcacgaatg cgctgcagtt ttacaacaga ttgctgctat
     6541 ccgtggagcg gtaaacggtc tgatgcggga agtgattaaa ggtcatctga cggaacacat
     6601 cgttcaccag ggggatgagc taaaacgtga agaagatctg gatgtcgttc tgaaggtgct
     6661 ggattcatat atcaaataat ttattaacgc gattgtaaaa ctgccgtttt tcctcgttta
     6721 caacgggggc gctggacatt accatcctcc tctgcgattt atcatcgcaa ccaaacgact
     6781 cggggtgccc ttctgcgtga aggctgagaa atacccgtat cacctgatct ggataatgcc
     6841 agcgtaggga agtcacggac caccaggtca ttgcttcttc acgttatggc aggagcaaac
     6901 tatgcaagtc gacctgctga gttcagcgca atctgcgcac gcgttacacc tttttcacca
     6961 acattcccct cttgtgcact gcatgaccaa tgatgtggtg caaaccttta ccgccaatac
     7021 cttgctggcg ctcggtgcat cgccagcgat ggttatcgaa accgaagagg ccagtcagtt
     7081 tgcggctatc gccagtgcct tgttgattaa cgttggcaca ctgacgcagc cacgcgcaca
     7141 ggcgatgcgt gctgccgttg agcaagcaaa aagctctcaa acacgctgga cgcttgatcc
     7201 agtagcggtt ggtgcgctcg attatcgccg ccatttttgt catgaacttt tatcttttaa
     7261 accggcagcg atacgtggta atgcttcgga aatcatggca ttagctggca ttgctaatgg
     7321 cggacgggga gtggatacca ctgacgccgc agctaacgcg atacccgctg cacaaacact
     7381 ggcacgggaa actggcgcaa tcgtcgtggt cactggcgag gtggattatg ttaccgatgg
     7441 acatcgtatc attggtattc acggtggcga tccgttaatg accaaagtgg taggaactgg
     7501 ctgtgcatta tcggcggttg tcgctgcctg ctgtgcgtta ccaggcgata cgctggaaaa
     7561 tgtcgcatct gcctgtcact ggatgaaaca agccggagaa cgcgcagtcg ccagaagcga
     7621 ggggccaggc agttttgttc cacatttcct tgatgcgctc tggcaattga cgcaggaggt
     7681 gcaggcatga aacgaattaa cgctctgacg attgccggta ctgatccgag tggtggtgcg
     7741 gggattcagg ccgatcttaa aaccttctcg gcacttggcg cttatggttg ctcagttatt
     7801 actgcactgg tggcgcaaaa tacccgtggc gtacagtcgg tgtatcgcat tgagcctgat
     7861 tttgtcgccg cccagctcga ttcggtgttc agcgatgtgc gaatcgatac cactaaaatc
     7921 ggtatgttgg cggaaaccga tattgttgaa gcggtggcag aacggttgca acgttatcag
     7981 atccaaaacg tggtactcga caccgttatg ctggcaaaaa gcggcgaccc gctgctttca
     8041 ccttcggcgg ttgctacgct gcgcagtcga ttattgccac aggtttcatt aataacgcca
     8101 aacttgcccg aagctgccgc cttgctcgac gcgccacacg cgcgcaccga acaggaaatg
     8161 ctggaacaag ggcgatcgct gttggcgatg ggctgtggcg cagtgctaat gaaaggtggt
     8221 catctggatg atgagcaaag cccggactgg ctgtttaccc gcgagggtga acaacggttt
     8281 accgcaccgc gcattatgac caaaaacacc cacggcactg gttgtacact ctctgcggcg
     8341 ttggctgcac tacgcccgcg ccatacaaac tgggctgaca ccgtacagga ggcaaaaatc
     8401 tggctttcat cggcgttagc ccaggccgac acgctggaag ttggtcacgg tattggtccg
     8461 gttcaccact tccacgcctg gtggtgagca ccgtactata gctgctttgg ttattgtcag
     8521 cctttggcac tgacattcag ggatgaaacc aatgcagcta agaattacca gtcgaaaaaa
     8581 actcaccgct ctattatgcg ctcttgggct aatttccatc gttgcaattt atccgcgtca
     8641 gacggtgaat tttttctatt cgacagcagt tcagattaca gactacatcc acttctacgg
     8701 ttatcgcccg gttaaatctt tcgctattcg cattcctgcc agttacacca ttcacggatt
     8761 agatgtttcg cgctggcagg agcgtatcga ctggcagcgg gtggcaaaaa tgcgcgacaa
     8821 cgatatccgc ttacagtttg cttttattaa ggctacggaa ggcgaaaagc tggtggaccc
     8881 ctatttttcg cgtaactggc aattaagccg cgaaaatggc ctgctgcgcg gggcgtatca
     8941 ttatttctcc ccgtcggtat ccgcttcagt tcaggcgcga ttatttctgc aaacggtgga
     9001 tttctcacaa ggcgatttcc ctgccgtgct ggacgtagaa gaacggggaa aattatcggc
     9061 aaaagaatta cgcaagcggg taagtcagtg gctaaaaatg gtcgaaaaac gcacgggaaa
     9121 aaagccgatt atttactcag gagccgtttt ttatcacacc aatctggcgg gctatttcaa
     9181 tgaatatccg tggtgggtgg ctcactatta tcaacgtcgt ccggacaatg acggcatggc
     9241 ctggcgcttc tggcagcatt ccgaccgtgg acaggtagat ggcatcaatg gtccggtgga
     9301 ttttaatgtg tttaatggca cggggatgag ttgcaggcat tcgttgatgg gattaaagaa
     9361 acgccttaaa taagaagcag tgatatactc ccaccagact ccctggcagg aaaaagacaa
     9421 atggaacaag cgcataccca gctgattgcc caactgaacg agcgtatttt agcggcggat
     9481 aacacgccgc tttatattaa gtttgccgaa acggtaaaaa atgccgtgcg cagcggggtg
     9541 ctggagcatg gcaatatttt gcccggtgag cgtgatttaa gtcagttaac cggcgtttcg
     9601 cgcattacgg tgcgcaaggc gatgcaggcg ctggaagaag agggcgtggt gacgcgttcg
     9661 cgcggttacg gcacgcagat caacaacatc ttcgaatact cgttgaaaga agcgagaggt
     9721 ttttctcagc aggtggtatt gcgcgggaaa aagcccgata cgctatgggt taataagcgt
     9781 gtcgtgaaat gtcccgaaga agttgcgcag cagctggcgg ccgaagcggg aagtgatgtc
     9841 tttttgctta agcgtattcg ctatgtcgat gaagaagcgg tatcgattga ggaatcgtgg
     9901 gttccggcgc atttaattca tgatgttgat gccatcggaa tttctcttta tgactatttc
     9961 cgcagccagc acatttaccc acaacgtacg cgttcccgcg ttagcgcccg gatgccggat
    10021 gccgagtttc agtcacatat tcagttagat agcaaaatac cggtgctggt gatcaagcaa
    10081 gtggcgcttg accaacagca acggcctatt gagtacagca ttagccactg tcgcagcgat
    10141 ctatacgttt ttgtgtgcga ggagtagttc ctcgcgcgtt ggcgcacaat caccgccccg
    10201 atgcccgaca acccacgacg ccactgcatt gcccagtaat acggcatccg ccagtggcag
    10261 accagaggcc agcccggcaa gtacgccacc tgcatgactg tcgcccgccc caatggtgtc
    10321 tacaacttgc gttggaaatg ccggaatgca gccagaagcg tcgttgctga aataccatgc
    10381 gccttcttta tcgagacgaa cgatcaacgg cgcggcaaat ttctcctgcc attgcttgcc
    10441 aagtgttgtt atctctgcgg ataaagcaaa acgttcggcg gcaatctcag cctcttgacg
    10501 attgagtgac actaaaggtc gacaggccat gatccgcgcc agtaatgcgt ccgggatatc
    10561 gccaatacgt gggcaaaaat cgataaacgg cgtcacgtct tgcagctttt ccagccattc
    10621 cactaacaat tcgccgcagg gcgaggccag ttgataaccg gaaaaataga gcaggttgcc
    10681 aggcgcaacg gttaatcgtg ccaaccattg gcgattccat tgattttcca caccgctgaa
    10741 tgacataaaa gtgcgttcgc catccggctc aaccagtgcc agacaccagc cgttatcgct
    10801 ttcggcgtta tcgatcaggc tgattaaccc ctctttcgcc atccggttgc gaataatctc
    10861 cgcccacacg ccctgaccga gcggcaaggc attacccgct tcgatgccga ggcgctttaa
    10921 cgccacggca atattcagtg cgcagccgcc aacgttaacg ctctgctgtt tcagttcgat
    10981 atcgcaccca cgccagggga gggcataagc gtcggcgatc acgtcaatga ccgccgcgcc
    11041 gacgaccatc accggctgac gcgtggttaa ttcaggcagc agtgtgtgta atcgagcgcc
    11101 gctcataccg cctcccgttg ttgacgatat ttcgccagcg ctgtggcata gcggttgaaa
    11161 tcaagctgat ttaccgcatc cagctccgcc tttaacgcag gatcgacagc gttaatgcca
    11221 tgcaacgcgc cgcaaatcgc cgtcgccata gcacctatgg tgtctgtgtc gccgccaaga
    11281 ttggcgcaca ggacggcgca gcggtttggg tcggtttgtg ccagttcaac tagcgcaatg
    11341 gcgcacggaa cggactcaat agtgctggtg cctgcgccaa cgacctggta aagctgttcg
    11401 ctggcggatt cggtgccgtc ggcattacgc acaattttca gcgcaatctc cagacgtgcc
    11461 gccagtgagg cgctgaacgt ggtggtgcgt ttttgctgtg catgtcgcgc aattgaaggc
    11521 aatgaatcaa caatcgctga ccagcgttct ccgtcaatgg cacgagaaat cgcccatgcg
    11581 atgactaccg cgcctgcaac cgccagatcg gatttatggg tcgggctgga cgccagcgcc
    11641 acatcatcaa taaaggaatc aacatcacgc gctggaagca aacaacctaa tggcgagacg
    11701 cgcatcgctg cgccgttggt tacgccgttg ttttccagtt cagcaacggg tttaccgtcg
    11761 cgaatggcgt taagcgcaat cttcgaggtc ggacctagta cgtttttgtt aaaggcgtcg
    11821 aaacgcaacg cccagtcgag aatattacgc ccaatcagat tcggatcgat cttgccttca
    11881 cattccagta acgcatccgc cagacacagg ccatcgaggt atcgtcggtg aattcggcgc
    11941 ggttaaaata acaggccgcg ttattctcct ttggtccagg cagaaaacgg tcaatccagc
    12001 caaagtgtgc tttaacgcgg ctgcgtggcc aaagctcgga gggcatcccc atcgcatccc
    12061 ctaacgcctg cccataaaga gcaccgagaa tacgttctgt tttcatttaa cttccccttg
    12121 aatcggaatt tacgggagca tagtaatgac agatattgcg cagttgcttg gcaaagacgc
    12181 cgacaacctt ttacagcacc gttgtatgac tattccttct gaccagcttt atctccccgg
    12241 acatgactac gtagaccgcg tgatgattga caataatcgc ccgccagcgg tgttacgtaa
    12301 tatgcagacg ttgtacaaca ccgggcgtct ggctggcaca ggatatcttt ctattctgcc
    12361 ggttgaccag ggcgttgagc actctgccgg agcttcattt gctgctaacc cgctctactt
    12421 tgacccgaaa aacattgttg aactggcgat cgaagcgggc tgtaactgtg tggcatcaac
    12481 ttacggcgtg ttggtgtcgg tatcgcggcg ctatgcgcat cgcattccat ttctcgtcaa
    12541 acttaatcac aacgagacgc taagttaccc gaatacctac gatcaaacgc tgtatgccag
    12601 cgtggagcag gccttcaaca tgggcgcggt ggcggttggt gcgactatct attttggctc
    12661 ggaagagtca cgtcgccaga ttgaagaaat ttctgcggcc tttgaacgcg cgcacgagct
    12721 gggcatggtg acagtgctgt gggcctattt gcgaaactcc gcctttaaga aagatggcgt
    12781 tgattaccat gtttccgccg acctgactgg tcaggcgaac catctggcgg caaccatagg
    12841 tgcagatatc gtcaaacaaa aaatggcgga aaataacggc ggctataaag cgattaatta
    12901 cggttatacc gacgatcgcg tgtacagcaa gttaaccagc gaaaacccga ttgatctggt
    12961 gcgttatcag ttagctaact gctatatggg ccgggctggg ttgataaact ccggcggtgc
    13021 tgcaggcggt gaaactgacc tcagcgatgc agtgcgtact gcggttatca acaaacgcgc
    13081 aggcggaatg gggctgattc ttggacgtaa ggcgttcaag aaatcgatgg ctgacggcgt
    13141 gaaactgatt aatgccgtgc aggacgttta tctcgatagc aaaattacta ttgcctgata
    13201 cgcctcatac gaacagcacc accccgtagg acggataaga cgtttacact gcatccggca
    13261 acctatgcct gatgcgacgc ggaagcgtct tatcatgcct acatagcact gccacgtatg
    13321 tttacaccgc atccggcata aaaacacgcg cactttgcta cggcttccct atcgggaggc
    13381 cgtttttttg cctttcactc ctcgaacaat tttcatattg ttgtttttgc gatcattatc
    13441 tcgacattta aaagcaaata atttcattat attttgaaat cgaaagcaaa cgacaagata
    13501 tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa cgcacagcgc ggcggttatg
    13561 cggttccggc attcaatatt cacaatctcg aaacgatgca agtggtggtg gaaaccgctg
    13621 ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg cacatttact catgctggta
    13681 cagaaaatct gttggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcaa
    13741 ttcatctcga ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc
    13801 gctcagtcat gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag
    13861 aggtggtgga tttttgccat cgctttgatg tcagcgtcga ggcggagctg gggcaacttg
    13921 gcggccagga agatgatgtg caagtcaatg aagtcgatgc gttgtacacc aaccccgcac
    13981 aggcgcgtga atttgccgag gcaaccggga ttgattccct ggcggtcgcc atcggcacgg
    14041 ctcacggtat gtatgccagc gcaccggtgc ttgatttttc tcgactggag aacattcgcc
    14101 agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg attatcgact aaggatattc
    14161 agcaaaccat caaactgggg atatgcaaaa tcaacgttgc aacggagctg aaaaatgcct
    14221 tcttgcagtc gttaaaaaat tacctgaccg agcaccccga agcgaccgat ccccgggatt
    14281 atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg
    14341 gctgcgaggg cagggcataa cgcacctgcc atttaacaag gaaaaaacat gaaaacgtta
    14401 attgcccggc ataaagctgg tgaacatatc ggcatatgtt cagtctgttc tgcccatccg
    14461 ttggttatcg aagcggcgct ggcatttgat cgcaacagca cgcgcaaagt gctgattgaa
    14521 gcaacgtcaa accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt
    14581 cgcgaatttg tttttacgat tgccgataaa gttgggtttg cacgcgaacg cattattctc
    14641 ggcggcgatc atctgggacc aaactgctgg cagcaagaaa atgcggatgc ggcgatggaa
    14701 aaatccgtcg agctggtaaa agcatatgtt cgtgctggct tcagtaaaat tcatcttgat
    14761 gcgtcaatgt cctgcgcggg ggataccata ccgttagcac cagaaacggt tgcggaacga
    14821 gctgctgtgc tttgctttgc tgcggaaagt gtggcgacag attgccagcg tgagcaactg
    14881 agctatgtca ttggcactga agttccggtt ccgggcggtg aggccagcgc cattcagtca
    14941 gtacacatca cccatgttga agatgccgcc aatactttac gtacgcatca aaaggccttt
    15001 attgcccgtg ggctgacaga ggcgttaaca cgtgtgattg ccatcgtggt gcagccgggt
    15061 gtggaatttg atcacagcaa tattatccat tatcagccgc aggaagcgca ggcgctggcg
    15121 caatggatag aaaacacccg aatggtttat gaagcacatt ctaccgatta ccagacccgg
    15181 acggcttatt gggaattagt ccgcgatcac tttgcaatat tgaaagtcgg tcccgcatta
    15241 acctttgctt tacgcgaggc gatatttgca ctggcacaaa ttgagcagga acttatcgcc
    15301 cccgaaaatc gcagcggttg cctggcggta attgaagaag tgatgctcga cgaaccgcaa
    15361 tactggaaaa aatattatcg cacgggtttt aacgattcat tactggatat tcgttacagc
    15421 ctgtcggatc gtattcgtta ttactggccg catagtcgga ttaaaaatag cgttgaaacg
    15481 atgatggtga atctgcaagg cgtggacatc ccactgggca tgattagtca gtatcttccc
    15541 aaacaatttg aacgcattca gtccggggaa ttatcagcaa tgccgcatca gcttattatg
    15601 aataaaattt atgatgtttt gcgcgcctat cgctacggct gtgcggaata aggacggtat
    15661 atgactaacc tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga agttttaacc
    15721 tatatcggta atgagatgct cgcgaaaggt gtggttcatg atacctggcc acaggcatta
    15781 attgccagag aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgcaata
    15841 ccgcattgtg aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat
    15901 aaagttcatt ttcagcaagc ggatgatgat aacgacgtgg cggtatcgtt ggttattgcg
    15961 ttgattgtgg aaaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta
    16021 caacagcccg atatcgtcga gacactaatt actcttcctg aaactcagtt aaaggaatac
    16081 ttcacaaagt atgttttaga ttcagacgaa taaatccctc tgtaacaata aaaaggacta
    16141 tttatgaaac gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg
    16201 gcggaagaaa ttaaagagtt gtgtcagagt cataatattc ctgttgaatt aatccagtgt
    16261 cgggttaatg aaatagaaac ctatatggat ggtgtgcatt tgatatgtac cactgccagg
    16321 gtggatcgta gttttggcga tattccgtta gttcacggca tgccttttgt ttctggtgtc
    16381 ggtatcgaag cattacaaaa taaaattctg actatcttac agggatgacc tatgttttca
    16441 gaagtcatgc gttatattct cgacctcggc cctacagtga tgctgccggt tgtcatcatt
    16501 attttttcta aaatattagg catgaaggca ggcgattgct ttaaagcggg tctgcatatc
    16561 gggattggct ttgttggcat tggccttgtg attggcttaa tgctggattc cattggtccg
    16621 gcggcgaaag cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg
    16681 ccgggctctt caccaatgac ctgggcgtcg caaattgcgc tggtggcgat tccgattgcg
    16741 atattggtta acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc
    16801 tggaatatct ggcatatgac cttcaccggc gcgttgctgc acctggcaac cggttcatgg
    16861 atgataggga tggcgggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg
    16921 tttgcccgcg atacccgaaa tttctttgag ctggaaggta ttgctattcc gcacggtacg
    16981 tcggcgtata tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc
    17041 gttaaccgaa ttaaatttag cgccgacgat attcagcgca aatttggccc ttttggcgaa
    17101 cctgtcaccg tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc
    17161 aaaggtgtat tgcagctggc ggtaaaaacg gcggcagtta tgctgctgat gccacggatg
    17221 attaaaccca tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag
    17281 gcgaagttcg gcggtcagga gttcctgatt ggcctggatc ccgcattact gctggggcat
    17341 acggcggtgg tatcggcaag cctgattttt attccgctca ccattttaat tgctgtttgt
    17401 gttccgggta atcaggtact gccgtttggc gatcttgcca ctatcggctt cttcgtggcg
    17461 atggcggtcg cggtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatt
    17521 atgagcatca ccctgtggat cgcgacgcaa actattggtt tgcacaccca accggcggct
    17581 aatgctgggg cgttaaaagc cgggggtatg gtggcttcaa tggatcaggg cggttctccc
    17641 attacctggt tactgattca ggttttctcc ccgcaaaata ttcccggttt cattattatc
    17701 ggcgcaattt atctgaccgg tattttcatg acctggcgta gagcgcgtgg ttttactaaa
    17761 caagagaaag ccgttctcgc agaataattt ttacctgagg gggaggttat cccccttaac
    17821 aa
//
LOCUS       DAZPEU010000070        17478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPEU010000070 DAZPEU010000000
VERSION     DAZPEU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.070"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(27..1310)
                     /gene="gltA"
                     /locus_tag="ACHU2L_002132"
     CDS             complement(27..1310)
                     /gene="gltA"
                     /locus_tag="ACHU2L_002132"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR1356985.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1516..1623)
                     /locus_tag="ACHU2L_002133"
     CDS             complement(1516..1623)
                     /locus_tag="ACHU2L_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356986.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2004..2408
                     /gene="sdhC"
                     /locus_tag="ACHU2L_002134"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2004..2408
                     /gene="sdhC"
                     /locus_tag="ACHU2L_002134"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1356987.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2402..2749
                     /gene="sdhD"
                     /locus_tag="ACHU2L_002135"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2402..2749
                     /gene="sdhD"
                     /locus_tag="ACHU2L_002135"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR1356988.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2749..4515
                     /gene="sdhA"
                     /locus_tag="ACHU2L_002136"
     CDS             2749..4515
                     /gene="sdhA"
                     /locus_tag="ACHU2L_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR1356989.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4531..5247
                     /gene="sdhB"
                     /locus_tag="ACHU2L_002137"
     CDS             4531..5247
                     /gene="sdhB"
                     /locus_tag="ACHU2L_002137"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR1356990.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5548..8349
                     /gene="sucA"
                     /locus_tag="ACHU2L_002138"
     CDS             5548..8349
                     /gene="sucA"
                     /locus_tag="ACHU2L_002138"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR1356991.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8364..9581
                     /gene="odhB"
                     /locus_tag="ACHU2L_002139"
     CDS             8364..9581
                     /gene="odhB"
                     /locus_tag="ACHU2L_002139"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR1356992.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            9675..10841
                     /gene="sucC"
                     /locus_tag="ACHU2L_002140"
     CDS             9675..10841
                     /gene="sucC"
                     /locus_tag="ACHU2L_002140"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR1356993.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10841..11710
                     /gene="sucD"
                     /locus_tag="ACHU2L_002141"
     CDS             10841..11710
                     /gene="sucD"
                     /locus_tag="ACHU2L_002141"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR1356994.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11813..12535)
                     /gene="mngR"
                     /locus_tag="ACHU2L_002142"
     CDS             complement(11813..12535)
                     /gene="mngR"
                     /locus_tag="ACHU2L_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR1356995.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12705..14621
                     /gene="mngA"
                     /locus_tag="ACHU2L_002143"
     CDS             12705..14621
                     /gene="mngA"
                     /locus_tag="ACHU2L_002143"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR1356996.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14639..17274
                     /gene="mngB"
                     /locus_tag="ACHU2L_002144"
                     /pseudo
     CDS             14639..17274
                     /gene="mngB"
                     /locus_tag="ACHU2L_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            17299..>17478
                     /locus_tag="ACHU2L_002145"
     CDS             17299..>17478
                     /locus_tag="ACHU2L_002145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1356997.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFVFIFTLKLRICG"
BASE COUNT         4234 a         4375 c         4756 g         4113 t
ORIGIN      
        1 aatatttaca acttagcaat caaccattaa cgcttgatat cgcttttaaa gtcgcgtttt
       61 tcatatcctg tatacagctg acgcggacgg gcaatcttca taccgtaact gtgcatttcg
      121 ctccagtggg cgatccagcc aacggtacgt gccattgcga aaatgacggt gaacatggaa
      181 gacggaatac ccatcgcttt caggatgata ccagagtaga aatcgacgtt cgggtacagt
      241 ttcttctcga taaagtacgg gtcgttcagc gcgatgtttt ccagctccat agccacttcc
      301 agcaggtcat ctttggtgcc cagctctttc agaacttcat ggcaggtttc acgcattacg
      361 gtggcgcgcg ggtcgtaatt tttgtacacg cggtgaccga agcccatcag gcggaaagaa
      421 tcatttttat ctttcgcacg acgaacaaat tccggaatgt gtttaacgga gctaatttct
      481 tccagcattt tcagcgccgc ttcgttagca ccaccgtgcg caggtcccca cagtgaagca
      541 atacctgctg cgatacaggc aaacgggttc gcccccgaag agccagcggt acgcacggtg
      601 gaggtagagg cgttctgttc atggtcagcg tgcaggatca gaatacggtc catagcacgt
      661 tccagaatcg gattaacttc atacggttcg cacggcgtgg agaacatcat attcaggaag
      721 ttaccggcat aggagagatc gttgcgcgga taaacaaatg gctgaccaat ggaatacttg
      781 taacacatcg cggccatggt cggcattttc gacagcaggc ggaacgcggc aatttcacga
      841 tgacgaggat tgttaacatc cagcgagtcg tgatagaacg ccgccagcgc gccggtaata
      901 ccacacatga ctgccattgg atgtgagtcg cgacggaaag cgtggaacag acgggtaatc
      961 tgctcgtgga tcatggtatg acgggtcacc gtagttttaa attcgtcata ctgttcctga
     1021 gtcggttttt caccattcag caggatgtaa caaacttcta ggtagttaga atcggtcgcc
     1081 agctgatcga tcgggaaacc gcggtgcagc aaaatacctt catcaccatc aataaaagta
     1141 attttagatt cgcaggatgc ggttgaagtg aagcctgggt caaaggtgaa cacacctttt
     1201 gaaccgagag tacggatatc aataacatct tgacccagcg tgcctttcag cacatccagt
     1261 tcaacagctg tgtccccgtt gaggttgagt tttgcttttg tatcagccat ttaaggtctc
     1321 cttagcgcct tattgcgtaa gactgccgga acttaaattt gccttcgcac atcaacctgg
     1381 ctttacccgt ttgttatttg gctcgccgct ctgtgaaaga ggggaaaacc tgggtacaga
     1441 gctctgggcg cttgcaggta aagaatccat tgatgacgaa taaatggcga atcaagtact
     1501 tagcaatccg aattattaaa cttgtctacc actaataact gtcccgaatg aattggtcaa
     1561 tacttccaca ctgttacata agttaatctt aggtgaaata ccgacttcat aacttttacg
     1621 cattatatgc ttttcctggt aatgtttgta acaactttgt tgaatgattg tcaaattaga
     1681 tgattaaaaa ttaaataaat gttgttatcg tgacctggat cactgttcag gataaaaccc
     1741 gacaaactat atgtaggtta attgtaatga ttttgtgaac agcctatact gccgccaggt
     1801 ctccggaaca ccctgcaatc ccgagccacc cagcgttgta acgtgtcgtt ttcgcatctg
     1861 gaagcagtgt tttgcatgac gcgcagttat ggaaaggacg ctgtctgacc cgcaagcaga
     1921 ccggaggaag gaaatcccga cgtctccagg taacagaaag ttaacctctg tgcccgtagt
     1981 ccccagggaa taataagaac agcatgtggg cgttattcat gataagaaat gtgaaaaaac
     2041 aaagacctgt taatctggac ctacagacca tccggttccc catcacggcg atagcgtcca
     2101 ttctccatcg cgtttccggt gtgatcacct ttgttgcagt gggcatcctg cagtggcttc
     2161 tgggtaccag cctctcttcc cctgaaggtt tcgagcaagc ttccgcgatt atgggcagct
     2221 tcttcgtcaa atttatcatg tggggcatcc ttaccgctct ggcgtatcac gtcgtcgtag
     2281 gtattcgcca catgatgatg gattttggct atctggaaga aacattcgaa gcgggtaaac
     2341 gctccgccaa aatctccttt gttattactg tcgtgctttc acttctcgca ggagtcctcg
     2401 tatggtaagc aacgcctccg cattaggacg caatggcgta catgatttca tcctcgttcg
     2461 cgctaccgct atcgtcctga cgctctacat catttatatg gtcggttttt tcgccaccag
     2521 tggcgaactg acatatgaag tctggatcgg tttcttcgcc tctgcgttca ccaaagtgtt
     2581 caccctgctg gcgctgtttt ctatcttgat tcatgcctgg atcggcatgt ggcaggtgtt
     2641 gaccgactac gttaaaccgc tggccttgcg cctgatgctg caactggtga ttgtcgttgc
     2701 actggtggtt tacgtgattt atggattcgt tgtggtgtgg ggtgtgtgat gaaattgcca
     2761 gtcagagaat ttgatgcagt tgtgattggt gccggtggcg caggtatgcg cgcggcgctg
     2821 caaatttccc agagcggcca gacctgtgcc ctgctctcta aagtcttccc gacccgttcc
     2881 cataccgttt ctgcgcaagg cggcattacc gttgcgctgg gtaataccca tgaagataac
     2941 tgggaatggc atatgtacga caccgtaaaa gggtcggact atatcggtga ccaggacgcg
     3001 attgaatata tgtgtaaaac cgggccggaa gcgattctgg aactcgaaca catgggcctg
     3061 ccgttctcgc gtctcgatga tggtcgtatc tatcaacgtc cgtttggcgg tcagtcgaaa
     3121 aacttcggcg gcgagcaggc ggcacgcact gcggcggctg ctgaccgtac cggtcacgca
     3181 ctgttgcaca cgctttatca gcagaacctg aaaaaccaca ccaccatttt ctccgagtgg
     3241 tatgcgctgg atctggtgaa aaaccaggat ggcgcggtgg tgggttgtac cgcactgtgc
     3301 atcgaaaccg gtgaagtggt ttatttcaaa gcccgcgcta ccgtgctggc gactggcgga
     3361 gcagggcgta tttatcagtc caccactaac gcccacatta acactggcga cggtgtcggc
     3421 atggctatcc gcgcaggcgt accggtgcag gatatggaaa tgtggcaatt ccacccgacc
     3481 ggcattgccg gtgcgggcgt actggtcacc gaaggttgcc gtggtgaagg cggttatctg
     3541 ctgaacaaac atggcgaacg ctttatggag cgttatgcgc cgaacgccaa agacctggcg
     3601 ggccgtgatg tggtggcgcg ttccatcatg attgaaatcc gtgaaggtcg cggctgtgat
     3661 ggtccgtggg ggccacacgc gaaactgaaa ctcgatcacc tgggtaaaga agttctcgaa
     3721 tcccgtctgc cgggtatcct ggagctttcc cgtaccttcg ctcacgtcga tccggtgaaa
     3781 gagccgattc cggttatccc aacctgtcac tacatgatgg gcggtattcc gaccaaagtt
     3841 accggtcagg cgctgactgt gaatgagaaa ggcgaagatg tggttgttcc gggactgttt
     3901 gccgttggtg aaatcgcttg tgtatcggta cacggcgcta accgtctggg cggcaactcg
     3961 ctgctggacc tggtggtctt tggtcgcgcg gcaggtctgc atctgcaaga gtctatcgcc
     4021 gagcagggcg cactgcacga tgccagcgag tctgatgttg aagcgtctct ggatcgcctg
     4081 aaccggtgga acaataatcg taacggtgaa gatccggtgg cgatccgtaa agcgctgcaa
     4141 gaatgtatgc agcataactt ctcggtcttc cgtgaaggtg atgcgatggc gaaagggctt
     4201 gagcagttga aagtgatccg cgagcgtctg aaaaatgccc gtctggatga cacttccagc
     4261 gagttcaaca cccagcgcgt tgagtgcctg gaactggata acctgatgga aacggcgtat
     4321 gcaacggctg tttctgccaa cttccgtacc gaaagccgtg gcgcacatag ccgcttcgac
     4381 ttcccggatc gtgatgatga aaactggctg tgccactccc tgtatctgcc tgagtcggaa
     4441 tccatgacgc gccgaagcgt caacatggaa ccgaaactgc gcccggcatt cccgccgaag
     4501 attcgtactt actaatgcgg agacaggaaa atgagactcg agttttcaat ttatcgctat
     4561 aacccggatg ttgatgatgc tccgcgtatg caggattaca ccctggaagc ggaagaaggt
     4621 cgcgacatga tgctgctgga tgcgcttatc cagctgaaag agaaagatcc cagcctgtcg
     4681 ttccgccgct cctgccgtga aggtgtgtgc ggttccgacg gtctgaacat gaacggcaag
     4741 aatggtctgg cctgtattac cccgatttcg gcactcaacc agccgggcaa gaagattgtg
     4801 attcgcccgc tgccaggttt accggtgatc cgcgatttgg tggtagacat gggacaattc
     4861 tatgcgcaat atgagaaaat taagccttac ctgttgaata atggacaaaa tccgccagct
     4921 cgcgagcatt tacagatgcc agagcagcgc gaaaaactcg acgggctgta tgaatgtatt
     4981 ctctgcgcat gttgttcaac ctcttgtccg tctttctggt ggaatcccga taagtttatc
     5041 ggcccggcag gcttgttagc ggcatatcgt ttcctgatcg atagccgtga taccgagact
     5101 gacagccgcc tcgacggttt gagcgatgca ttcagcgtat tccgctgtca cagcatcatg
     5161 aactgcgtca gtgtatgtcc gaaggggctg aacccgacgc gcgccatcgg ccatatcaag
     5221 tcgatgttgt tgcaacgtaa tgcgtaaact gtaggcctga taagacgcgc aagcgttgca
     5281 tcaggcaacc agtgccggat gcggcgtgaa cgccttatcc ggcctacaag tcattacccg
     5341 caggcctgat aagcgcagcg catcaggcgt aacaaagaaa tgcaggaaat ctttaaaaac
     5401 tgcccctgac actaagacag tttttaaagg ttccttcgcg agccactacg tagacaagag
     5461 ctcgcaagtg aaccccggca cgcacatcac tgtgcgtggt agtatccacg gcgaagtaag
     5521 cataaaaaag atgcttaagg gatcacgatg cagaacagcg ctttgaaagc ctggttggac
     5581 tcttcttacc tctctggcgc aaaccagagc tggatagaac agctctatga agacttctta
     5641 accgatcctg actcggttga cgctaactgg cgttcgacgt tccagcagtt acctggtacg
     5701 ggagtcaaac cggatcaatt ccactctcaa acgcgtgaat atttccgccg cctggcgaaa
     5761 gacgcttcac gttactcttc aacgatctcc gaccctgaca ccaatgtgaa gcaggttaaa
     5821 gtcctgcagc tcattaacgc ataccgcttc cgtggtcacc agcatgcgaa tctagatccg
     5881 ctgggactgt ggcagcaaga taaagtggcc gatctggatc cgtctttcca cgatctgacc
     5941 gaagcagact tccaggaaac cttcaacgtc ggttcatttg ccagcggcaa agaaaccatg
     6001 aaactcggcg agcttatttc cgccctcaag caaacctact gcggcccgat tggtgccgag
     6061 tatatgcaca tcaccagcac cgaagaaaaa cgctggatcc aacagcgtat cgagtctggt
     6121 cgcgcgactt tcaatagcga agagaaaaaa cgcttcttaa gcgaactgac cgccgctgaa
     6181 ggccttgagc gttacctcgg cgcaaaattc cctggcgcaa aacgcttctc gctggaaggc
     6241 ggtgacgcgt taatcccgat gcttaaagag atgatccgcc acgctggcaa cagcggcacc
     6301 cgcgaagtgg ttctcgggat ggcgcaccgt ggtcgtctga acgtgctggt gaacgtgctg
     6361 ggtaaaaaac cgcaagactt gttcgacgag ttcgccggta aacataaaga acacctcggc
     6421 acaggcgacg tgaaatacca catgggcttc tcgtctgact tccagaccga tggcggcctg
     6481 gtgcacctgg cgctggcgtt taacccgtct caccttgaga ttgtaagccc ggtcgttatc
     6541 ggctctgttc gtgcccgtct ggacagactt gatgagccga acagcaacaa agtgctgcca
     6601 atcaccatcc acggtgacgc cgcagtgacc gggcagggcg tggttcagga aactctgaac
     6661 atgtcgaaag cgcgtggtta tgaagttggc ggtacggtac gtatcgttat caacaaccag
     6721 gttggtttca ccacctctaa cccgctggat gcccgttcta caccgtactg tactgatatc
     6781 ggtaagatgg ttcaggcacc gattttccac gttaacgcgg atgatccgga agccgttgcc
     6841 tttgtgaccc gtctggcgct cgatttccgt aacaccttta aacgtgatgt cttcatcgac
     6901 ctggtatgct accgccgtca cggccacaac gaagccgacg agccgagcgc aacccagcca
     6961 ctgatgtatc agaaaatcaa aaaacatccg acaccgcgca aaatctacgc tgacaagctg
     7021 gagcaggaaa aagtggcgac gctggaagat gccaccgaga tggttaacct gtaccgcgat
     7081 gcgctggatg ctggcgattg cgtagtggca gagtggcgtc cgatgaacat gcactctttc
     7141 acctggtcgc cgtacctcaa ccacgaatgg gacgaagagt acccgaacaa agttgagatg
     7201 aagcgcctgc aggaactggc taaacgtatc agtacggtac cagaagcagt tgaaatgcag
     7261 tctcgcgttg ccaagattta tggcgatcgc caggcgatgg cagccggtga gaaactgttc
     7321 gactggggcg gtgcggaaaa cctcgcttac gccacgctgg ttgatgaagg cattccggtt
     7381 cgcctgtcgg gtgaagactc cggtcgcggt accttcttcc accgccacgc ggtgatccac
     7441 aaccagtcta acggttccac ttacacgccg ctgcaacaca tccataacgg tcagggcgcg
     7501 ttccgtgtct gggactccgt actgtcagaa gaagccgtgc tggcgtttga atatggttat
     7561 gccaccgcag aaccacgcac cctgaccatc tgggaagcgc agttcggtga cttcgccaac
     7621 ggtgcgcagg tggttatcga ccagttcatc tcctctggcg agcagaaatg gggccggatg
     7681 tgtggtctgg tgatgttgct gccgcacggt tacgaagggc aggggccgga gcactcctcc
     7741 gcgcgtctgg aacgttatct gcaactttgt gctgagcaaa acatgcaggt ttgcgtaccg
     7801 tctaccccgg cacaggttta ccacatgctg cgtcgtcagg cgctgcgcgg gatgcgtcgt
     7861 ccgctggtcg tgatgtcgcc gaaatccctg ctgcgtcatc cgctggcggt ttccagcctc
     7921 gaagaactgg cgaacggcac cttcctgcca gccatcggtg aaatcgacga gcttgatccg
     7981 aagggcgtga agcgcgtagt gatgtgttct ggtaaggttt attacgacct gctggaacag
     8041 cgtcgtaaga acaatcaaca cgatgtcgcc attgtgcgta tcgagcaact ctacccgttc
     8101 ccgcataaag cgatgcagga agtgttgcag cagtttgctc acgtcaagga ttttgtctgg
     8161 tgccaggaag agccgctcaa ccagggcgca tggtactgca gccagcatca tttccgtgaa
     8221 gtgattccgt ttggggcttc tctgcgttat gcaggccgcc cggcctccgc ctctccggcg
     8281 gtagggtata tgtccgttca ccagaaacag caacaagatc tggttaatga cgcgctgaac
     8341 gtcgaataaa taaaggatac acaatgagta gcgtagatat tctggtccct gacctgcctg
     8401 aatccgtagc cgatgccacc gtcgcaacct ggcataaaaa acccggcgac gcagtcgtac
     8461 gtgatgaagt gctggtagaa atcgaaactg acaaagtggt actggaagta ccggcatcag
     8521 cagacggcat tctggatgcg gttctggaag atgaaggtac aacggtaacg tctcgtcaga
     8581 tccttggtcg cctgcgtgaa ggcaacagcg ccggtaaaga aaccagcgcc aaatctgaag
     8641 agaaagcgtc cactccggcg caacgccagc aggcgtctct ggaagagcaa aacaacgatg
     8701 cgttaagccc ggcgatccgt cgcctgctgg ctgaacacaa tctcgacgcc agcgccatta
     8761 aaggcaccgg tgtgggtggt cgtctgactc gtgaagatgt ggaaaaacat ctggcgaaag
     8821 ccccggcgaa agagtctgct ccggcagcgg ctgctccggc ggcgcaaccg gctctggctg
     8881 cacgtagtga aaaacgtgtc ccgatgactc gcctgcgtaa gcgtgtggca gagcgtctgc
     8941 tggaagcgaa aaactccacc gccatgctga ccacgttcaa cgaagtcaac atgaagccga
     9001 ttatggatct gcgtaagcag tacggtgaag cgtttgaaaa acgccacggc atccgtctgg
     9061 gctttatgtc cttctacgtg aaagcggtgg ttgaagccct gaaacgttac ccggaagtga
     9121 acgcgtctat cgacggcgat gacgtggttt accacaacta tttcgacgtc agcatggcgg
     9181 tttctacgcc gcgcggcctg gtgacgccgg ttctgcgtga tgtcgatacc ctcggcatgg
     9241 cagacatcga gaagaaaatc aaagagctgg cagtcaaagg ccgtgatggc aagctgacgg
     9301 ttgaagatct gaccggtggt aacttcacca tcaccaacgg tggtgtgttc ggttccctga
     9361 tgtctacgcc gatcatcaac ccgccgcaga gcgcaattct gggtatgcac gctatcaaag
     9421 atcgtccgat ggcggtgaat ggtcaggttg agatcctgcc gatgatgtac ctggcgctgt
     9481 cctacgatca ccgtctgatc gatggtcgcg aatccgtggg cttcctggta acgatcaaag
     9541 agttactgga agagccgacg cgtctgctgc tggacgtgta gtagtttaag cttcacctgc
     9601 actgtagacc gacgtggata gggtgtcggt ctacggttta aaagataacg attactgaag
     9661 gatggacaga acacatgaac ttacatgaat atcaggcaaa acaactgttt gcccgctatg
     9721 gcttaccagc accggtgggt tatgcctgta ctactccgcg cgaagcagaa gaagccgctt
     9781 caaaaatcgg tgccggtccg tgggtagtga aatgtcaggt tcacgctggt ggccgcggta
     9841 aagcgggcgg tgtgaaagtt gtaaacagca aagaagacat ccgtgctttt gcagaaaact
     9901 ggctgggcaa gcgtctggta acgtatcaaa cagatgccaa tggccaaccg gttaaccaga
     9961 ttctggttga agcggcgacc gatatcgcta aagagctgta tctcggtgcc gttgtagacc
    10021 gtagttcccg tcgcgtggtc tttatggcct ccaccgaagg cggcgtggaa atcgaaaaag
    10081 tggcggaaga aactccgcac ctgatccata aagttgcgct tgatccgctg actggcccga
    10141 tgccgtatca gggacgcgag ctggcgttca aactgggtct ggaaggtaaa ctggttcagc
    10201 agttcaccaa aatcttcatg ggcctggcga ccattttcct ggagcgcgac ctggcgctga
    10261 tcgaaatcaa cccgctggtc atcaccaaac agggcgatct gatttgcctc gacggcaaac
    10321 tgggcgctga tggcaacgcc ctgttccgcc agcctgatct gcgcgaaatg cgtgaccagt
    10381 cgcaggaaga tccgcgtgaa gcacaggctg cacagtggga actgaactac gttgcgctgg
    10441 acggtaacat cggttgtatg gttaacggcg caggtctggc gatgggtacg atggacatag
    10501 ttaaactgca cggcggcgaa ccggctaact tcctggacgt tggcggcggc gcaaccaaag
    10561 aacgtgtaac cgaagcgttc aaaatcatcc tctctgacga caaagtgaaa gccgttctgg
    10621 ttaacatctt cggcggtatc gttcgttgcg acctgatcgc tgacggtatc atcggcgcgg
    10681 tagcggaagt gggtgttaac gtaccggtcg tggtacgtct ggaaggtaac aacgccgaac
    10741 tcggcgcgaa gaaactggct gacagcggcc tgaatattat tgcagcaaaa ggtctgacgg
    10801 atgcagctca gcaggttgtt gccgcagtgg aggggaaata atgtccattt taattgataa
    10861 aaacaccaag gttatctgcc agggctttac cggtagccag gggactttcc actcagaaca
    10921 ggccattgca tacggcacta aaatggttgg tggcgtaact ccaggtaaag gcggcactac
    10981 ccacctcggc ctgccggtgt tcaacaccgt gcgtgaagct gttgctgcta ctggcgcttc
    11041 cgcgtctgtt atttacgtac cagcaccgtt ctgcaaagac tccattctgg aagctatcga
    11101 tgcaggcatc aaactgatta tcaccatcac tgaaggcatc ccgacgctgg atatgctgac
    11161 cgtgaaagtg aaactggacg aagcaggcgt tcgtatgatc ggcccgaact gcccaggcgt
    11221 tatcaccccg ggtgaatgca aaattggtat ccagccgggt cacattcaca aaccgggtaa
    11281 agtgggtatc gtttcccgtt ccggtacact gacctatgaa gcggttaaac agaccacgga
    11341 ttacggtttc ggtcagtcga cctgtgtcgg tatcggcggt gacccgatcc ctggctctaa
    11401 ctttatcgac attctcgaaa tgttcgaaaa agatccgcag accgaagcga tcgtgatgat
    11461 cggtgagatc ggcggtagcg ctgaagaaga agcggcagcg tacatcaaag agcatgttac
    11521 caagccagtt gtgggttaca tcgctggtgt gactgcgccg aaaggcaaac gtatgggcca
    11581 cgcgggtgcg atcattgccg gtgggaaagg gactgcggat gagaaattcg ctgctctgga
    11641 agccgcaggc gtgaaaaccg ttcgcagcct ggcggatatc ggtgaagcac tgaaaactgt
    11701 tctgaaataa atatctgtaa taagaaatag ccctcgccgc ttccctctac aggaatggcg
    11761 aagggctgtc ggtttcgaca tggttggcca tcgtatgatg gcctttttgt gcttatcgcg
    11821 atgattttcg ctgcgctatc agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc
    11881 tatattcaaa cacccgacca tcaactaaat atccacgcga tactttttca agaatcggct
    11941 ttgtctggct gatattaagc agacggctca tctcttcggt tggcatcaga ggaatgattt
    12001 cctgttcgct acgatcgata accatcttct tcacttcttc aataaagtga tatttcgaat
    12061 tttccatgac ctgccaggtg agatccggga acaacgcaag cggcatccag gtttcttcca
    12121 gcgccattgg cttttgcttg cgatagcgca cgcgcttcac atgccaaaca cgatcctgca
    12181 cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg aatcacttca aatatcagaa
    12241 cttcactgtg tgtatcgacg tgacggtccg acagtttttc atcaaaactg gttaactgaa
    12301 aaatatcgta attgacccgc tcttctttga cgtaagtccc gctgccctga atgctttcga
    12361 ggatctgttg ctcgactaac tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa
    12421 actctgtttg taatgccgat tcggtaggta acgcatcgcc aggtttaagc tcgccacgcg
    12481 caatttgctc acgaatgcga tcagcaatct gtcggtataa gggtttgtgt cccattattt
    12541 agcatctcat taatacgaat ttagccatta tgcccgataa actcatcctg taaacaatac
    12601 aaataaaatg caaataattt caatcaagtg aaattgatta cgcaatggta ttgttttgtc
    12661 gggcacactg gcgcgactat aaaaacgatc aagtgaggat catgatgaac ctgacgactc
    12721 tgacccaccg cgatgcgttg tgtctgaatg cgcgctttac cagccgtgaa gaggccattc
    12781 acgcgttgac tcaacgtctt gctgctctgg ggaaaatttc cagtactgag caatttctga
    12841 aagaagtgta tcgccgtgaa agccttggcc cgaccgcctt aagtgaaggg ttggctgtgc
    12901 cgcatggcaa aactgctgcg gtaaaagaag cggcgtttgc agtcgccaca ctcagcgagc
    12961 cgcttcagtg ggaaggcgtg gatggcccgg aagcggttga tttagtggtg ttgctggcga
    13021 ttcccctcaa tgaagcgggt actacgcaca tgcaactgct gacagcgctg accacgcgcc
    13081 tggcggatga tgagattcgg gcgcgtatac agtcggcgac gacgcctgat gaattgctct
    13141 cggcgctgga tgacaaggga ggcacgcaac cttctgcctc tttttccaac gcgccaacta
    13201 tcgtttgcgt aacggcctgc ccggcgggta ttgctcacac ctacatggct gcggagtatc
    13261 tggaaaaagc cggacgcaaa ctcggcgtga atgtctacgt tgaaaagcaa ggtgctaacg
    13321 gtattgaagg gcgtttaacg gcggatcaac tcaatagtgc aactgcctgt atttttgcgg
    13381 ctgaagtcgc catcaaggag agtgagcgtt ttaacggcat tcctgcgctt tctgtgcctg
    13441 ttgccgagcc gattcgccat gcagaagcgt tgatccaaca atcgcttacc ctcgagcgtg
    13501 gcgatgagac gcgtacctta cagcaagata cgcaaccggt gaaaagcgtc aaaacggagc
    13561 tgaaacaggc actgttgagc gggatctctt ttgccgtacc gttgattgtc gcggggggga
    13621 cggtgctggc ggtcgcggta ttactgtcgc aaatcttcgg gctacaagat ctgtttaatg
    13681 aagaaaactc ctggctgtgg atgtaccgca aactgggcgg cgggctgctc ggaattttga
    13741 tggtaccggt gctggcagcc tataccgcct attcgctggc agataaaccg gcgttagcgc
    13801 caggctttgc ggctggactt gccgccaaca tgatcggctc cgggtttctc ggcgcggtcg
    13861 ttggcggatt gatagccggt tacttgatac gctggatgaa aaatcacttg cgtcttagca
    13921 gtaaattcaa tggcttcctg actttttatc tctacccggt gctcggtaca ttgggagcgg
    13981 gcagtctgat gctgtttgtg gtgggggaac ctgtcgcctg gatcaataac tcgcttaccg
    14041 cctggctgaa cggtctgtca ggaagtaacg cgctgttgct gggtgccatt ctcggtttta
    14101 tgtgttcctt tgaccttgga gggccagtga ataaagccgc ttatgcattc tgcctgggcg
    14161 caatggcgaa cggcgtttac ggcccgtatg ccattttcgc ctccgtcaaa atggtttcgg
    14221 catttaccgt aaccgcttcc acgatgctcg caccgcgcct gtttaaagag tttgaaattg
    14281 agaccgggaa atccacctgg ctgttagggc tggcaggtat taccgaaggg gcgatcccga
    14341 tggcgattga agatccgctg cgggttattg gttcgtttgt gctgggctct atggtaacgg
    14401 gcgctattgt cggtgcgatg aatatcggcc tttcgacacc cggtgctggc attttctcgc
    14461 tctttttact tcatgataat ggcgcgggcg gtgttatggc ggcaattggc tggtttggtg
    14521 cggcattggt gggggctgca atctcgactg caattctcct gatctggcgg cgtcacgcgg
    14581 ttaagcatgg caactatctg actgatggcg taatgccata aacaaaaagg aaacgacgat
    14641 gaaagcggta tctcgcgttc acatcacccc gcatatgcac tgggatcggg agtggtattt
    14701 caccaccgaa gagtcacgca ttctgctggt caataatatg gaagagatcc tgtgccgact
    14761 ggaacaggac aacgaataca aatattacgt actcgacggg caaacggcga tcctcgaaga
    14821 ttatttcgcg gtgaaaccgg aaaacaaaga ccgcttgaag aaactggtag aagccggcaa
    14881 gttgattatc ggcccctggt atacccagac cgataccacg attgtttctg cggaatccat
    14941 cgtccgtaat ctgatgtacg gaatgcgtga ctgcctcgcg tttggcgagc cgatgaaaat
    15001 aggttattta cccgattcct ttggcatgtc cgggcaactg ccgcatatct acaatggatt
    15061 tggcattacc tgcaccatgt tctggcgtgg atgttctgag cgccacggta cggataaaac
    15121 cgagtttttg tggcaaagca gtgacggtag cgaagtgacg gcgcaggtga tgccgttggg
    15181 ttatgccatc ggtaagtact tacctgcgga cgaaaacgga ttacgcaaac gcctcgacag
    15241 ttattttgac gtgctggaaa aagcgtctgt aaccaaagag attttgctgc cgaatgggca
    15301 tgatcagatg ccattgcagc aaaatatctt cgaagtgatg gagaaactgc gcgaaattta
    15361 tcctcaacga aagtttgtga tgagccgctt tgaagaggta tttgagaaga tcgaagcgca
    15421 gcgagataat ctggcaaccc tgaaagggga atttattgat ggcaaatata tgcgcgtgca
    15481 tcgcaccatc ggttccacgc gcatggatat caaagttgcc cacgcacgta ttgaaaataa
    15541 gattgttaat ctgctggaac cgctggcaac gctggccagg acgttgggtt ttggatacca
    15601 ccacggcttg ctggagaaaa tgtggaaaga gatcttaaaa aatcatgccc acgacagcat
    15661 cggctgctgc tgtcgtgaca aagttcatcg cgaaatcgtc gcccgcttcg aactggctga
    15721 agacatggcg gataatctgc tgcgtttcta tatgcgtaaa atcgccgaca acatgccgca
    15781 gagcgacgcc gacaaactcg tcctgtttaa cctgatgccc tggccgcggg aagaagtgat
    15841 caacaccact gtgcggctgc gcgccagcca gtttaatcta cgagacgatc gcggtcagcc
    15901 tgtaccgtat tttattcgcc atgcccgtga gatcgatcca ggcctaatcg atcggcaaat
    15961 agttcattac ggtaattacg atccctttat ggagtttgat atacagatca accagattgt
    16021 cccttctatg ggctatcgca cgctttatat cgaagcgaat cagcctggca acgtgattgc
    16081 ggcaaaaagt gacgctgaag ggatactgga aaatgctttc tggcaaattg cgctcaatga
    16141 ggatggaacg ctacaactgg tagataaaga tagcggtgtg cgctatgacc gggtgttgca
    16201 aattgaagaa agctctgatg atggtgatga atatgactat tcacccgcaa aagaagagtg
    16261 ggtaattact tcggcgaatg cgaaaccgca atgcgagatt attcatgagg cctggcagag
    16321 cagggctgtt atccgctatg aaatggcggt gccgcgtaat ctgcaacaac gcagcgcaag
    16381 acaatccact ggcagagtag atgtcgagtt ggttgtcact cttagtcata acaacaggcg
    16441 cattgatgtg gatatcaacc ttgataacca ggctgacgat catcgccttc gcgtcctgat
    16501 ccctacatct tttaacaccg atagtgttct ggcagatacc cagtttggtt cgctaacgcg
    16561 ccccgtgaac gacagtgcaa tgaacaactg gcagcaagaa agctggaaag aagcgccggt
    16621 tccggtatgg aatatgctca actatgcagc cttacaggaa gggcgtaacg gcatggctgt
    16681 ctttagcgaa gggctacgtg aatttgaagt catcggtgaa gagaagaaaa cctttgccat
    16741 tacgttgctg cgcggcgtgg gcttactggg gaaagaagat ctgcttttaa ggcctaggcg
    16801 gccttcggga attaaaatgc cagtcccgga ctcacaacta cgtggtctgc tttcttgtcg
    16861 cctaagttta ttgagttata ccggtacgcc aaccgccgct ggtgtagctc agcaggcgcg
    16921 agcatggctg actccagtac agtgttacaa caaaatccca tgggatgcga tgaagctcaa
    16981 caaagccgga ttcaacgtgc cggaaagtta tagtttgttg aaaatgcccc cagtgggatg
    17041 cccctgataa gcgcacttaa gaaagctgaa gaccgacaag aagtgatttt acggctgttt
    17101 aatccggctg aatcagcaac ctgtgatgcg actgttgctt tcagtcgcga ggtgatttct
    17161 tgctcagaaa cgatgatgga tgaacacatt accaccgagg aaaatcaagg ttcaaatcta
    17221 tcggggcctt ttttacccgg ccagtcacgg acgttcagtt accggcttgc ctgaatagca
    17281 atcaaaccga agccacatat gcgcggccag attgttgaca aagggcgctt tgttcatgcc
    17341 ggatacggca tgaacgcttt attcggtcta caaaagcagg caaattcaat atattgcaga
    17401 gatgatgtag acactggcaa gcgtagcgca tcaggtaatt ttgtgtttat cttcactctc
    17461 aagctacgta tatgtggc
//
LOCUS       DAZPEU010000071        17144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPEU010000071 DAZPEU010000000
VERSION     DAZPEU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.071"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..510
                     /locus_tag="ACHU2L_002146"
     CDS             <1..510
                     /locus_tag="ACHU2L_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR1356998.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHU2L_002147"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHU2L_002147"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR1356999.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHU2L_002148"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHU2L_002148"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR1357000.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACHU2L_002149"
     CDS             complement(2878..3075)
                     /locus_tag="ACHU2L_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1357001.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHU2L_002150"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHU2L_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR1357002.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHU2L_002151"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHU2L_002151"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR1357003.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHU2L_002152"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHU2L_002152"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR1357004.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHU2L_002153"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHU2L_002153"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR1357005.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHU2L_002154"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHU2L_002154"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR1357006.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHU2L_002155"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHU2L_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACHU2L_002156"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACHU2L_002156"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1357007.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHU2L_002157"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHU2L_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR1357008.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACHU2L_002158"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACHU2L_002158"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR1357009.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACHU2L_002159"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACHU2L_002159"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR1357010.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACHU2L_002160"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACHU2L_002160"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1357011.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHU2L_002161"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHU2L_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         4007 a         4874 c         4453 g         3810 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta attatgggta
    17101 atgactccaa cttactgata gtgttttatg ttcagataat gccc
//
LOCUS       DAZPEU010000072        17137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPEU010000072 DAZPEU010000000
VERSION     DAZPEU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.072"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            7..569
                     /gene="srlA"
                     /locus_tag="ACHU2L_002162"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7..569
                     /gene="srlA"
                     /locus_tag="ACHU2L_002162"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            566..1525
                     /gene="srlE"
                     /locus_tag="ACHU2L_002163"
                     /gene_synonym="gutE"
     CDS             566..1525
                     /gene="srlE"
                     /locus_tag="ACHU2L_002163"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR1357012.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1536..1907
                     /gene="srlB"
                     /locus_tag="ACHU2L_002164"
                     /gene_synonym="gutB"
     CDS             1536..1907
                     /gene="srlB"
                     /locus_tag="ACHU2L_002164"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR1357013.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1911..2690
                     /gene="srlD"
                     /locus_tag="ACHU2L_002165"
     CDS             1911..2690
                     /gene="srlD"
                     /locus_tag="ACHU2L_002165"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR1357014.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2795..3154
                     /gene="gutM"
                     /locus_tag="ACHU2L_002166"
     CDS             2795..3154
                     /gene="gutM"
                     /locus_tag="ACHU2L_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR1357015.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3221..3994
                     /gene="srlR"
                     /locus_tag="ACHU2L_002167"
                     /gene_synonym="gutR"
     CDS             3221..3994
                     /gene="srlR"
                     /locus_tag="ACHU2L_002167"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR1357016.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            3987..4952
                     /gene="gutQ"
                     /locus_tag="ACHU2L_002168"
     CDS             3987..4952
                     /gene="gutQ"
                     /locus_tag="ACHU2L_002168"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR1357017.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4949..6463)
                     /gene="norR"
                     /locus_tag="ACHU2L_002169"
     CDS             complement(4949..6463)
                     /gene="norR"
                     /locus_tag="ACHU2L_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR1357018.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6650..8089
                     /gene="norV"
                     /locus_tag="ACHU2L_002170"
     CDS             6650..8089
                     /gene="norV"
                     /locus_tag="ACHU2L_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR1357019.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8086..9219
                     /gene="norW"
                     /locus_tag="ACHU2L_002171"
     CDS             8086..9219
                     /gene="norW"
                     /locus_tag="ACHU2L_002171"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR1357020.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(9347..11599)
                     /gene="hypF"
                     /locus_tag="ACHU2L_002172"
     CDS             complement(9347..11599)
                     /gene="hypF"
                     /locus_tag="ACHU2L_002172"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR1357021.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11752..12279)
                     /gene="hydN"
                     /locus_tag="ACHU2L_002173"
     CDS             complement(11752..12279)
                     /gene="hydN"
                     /locus_tag="ACHU2L_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR1357022.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12428..13438)
                     /gene="ascG"
                     /locus_tag="ACHU2L_002174"
     CDS             complement(12428..13438)
                     /gene="ascG"
                     /locus_tag="ACHU2L_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR1357023.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(13500..13571)
                     /locus_tag="ACHU2L_002175"
                     /pseudo
     CDS             complement(13500..13571)
                     /locus_tag="ACHU2L_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13581..15290)
                     /gene="hycE"
                     /locus_tag="ACHU2L_002176"
     CDS             complement(13581..15290)
                     /gene="hycE"
                     /locus_tag="ACHU2L_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR1357024.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(15308..16231)
                     /gene="hycD"
                     /locus_tag="ACHU2L_002177"
     CDS             complement(15308..16231)
                     /gene="hycD"
                     /locus_tag="ACHU2L_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR1357025.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(16234..>17137)
                     /locus_tag="ACHU2L_002178"
     CDS             complement(16234..>17137)
                     /locus_tag="ACHU2L_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR1357026.1"
                     /translation="GIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGH
                     RDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVAR
                     LLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAIC
                     CVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRL
                     PSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGS
                     ALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         4049 a         4836 c         4526 g         3726 t
ORIGIN      
        1 agaacaatga tagaaaccat tactcatggt gcagagtggt ttatcgggct gttccaaaag
       61 ggcggagagg tgtttaccgg gatggtgacc ggcattcttc cgctgttgat tagcctgcag
      121 gttatcatga acgcactgat taattttatc ggtcagcatc gtattgaacg ttttgctcaa
      181 cgttgcgccg gtaaccctgt ttcccgttac ctactgttac cgtgcattgg cacgtttgtc
      241 ttttgcaatc cgatgacctt aagcctgggt cgctttatgc cggaaaagta caaacccagc
      301 tactacgcgg cggcctctta tagctgccac tcaatgaatg gcctcttccc ccatatcaac
      361 cctgcgaact gtttgtttat cttggcattg ccagcggtct gacaacgctg aacctgccac
      421 ttggcccact ggcggtgagt tatctgctgg ttggtctggt caccaatttc ttccgcggct
      481 gggtgaccga tctgaccacc gccatttttg agaaaaagat gggcattcaa cttgaacaaa
      541 aagttcacct ggcaggagca acatcatgac gcgtattcgg atcgaaaaag gaacgggtgg
      601 ctggggcggc ccgcttgagc tggaagccac gccgggcaaa aaaatcgtct atatcaccgc
      661 cggtacccgg cctgcgattg ttgacaaact ggcacaactt actggctggc aggctattga
      721 cggatttaaa gaaggtgaac ccgcggaggc ggaaattggt gtcgcggtaa tcgactgtgg
      781 cggcacatta cgctgcggca tctatccgaa acgacgtatt cccaccatta atatccactc
      841 gacgggcaag tccggtccgc tggcgcagta cattgtggaa gatatttatg tctctggcgt
      901 aaaagaagaa aacatcactg tagtgggtga tgcgacacca caaccctctt ccgtgggccg
      961 tgactatgac accagtaaga aaatcaccga acaaagcgat ggtttactgg cgaaggtggg
     1021 aatgggcatg gggtccgccg ttgccgtgtt gtttcagtct ggtcgtgaca ccatcgacac
     1081 tgtattaaaa accattctgc cgtttatggc attcgtctcg gcgctcattg gcatcattat
     1141 ggcttctggc cttggtgact ggattgccca cggtcttgct ccgctagcga gccatccact
     1201 gggtctggtc atgctggcgc tcatctgctc cttcccactg ctttcacctt tcctcggccc
     1261 aggcgcagtt atcgcacagg ttatcggcgt attgattggc gtgcagattg gtctcggcaa
     1321 tattccgccg catctggctt taccggcact gtttgccatc aacgcgcagg cggcttgcga
     1381 cttcatcccg gtcggtttgt cgctggcgga agcccgtcag gacacggttc gcgtcggtgt
     1441 cccttctgta ctggtgagcc gctttttaac cggcgcgccg actgtactga tcgcctggtt
     1501 tgtctccggt tttatctatc aatagaggct gaaacatgac cgttatttat cagaccacca
     1561 tcacccgtat cggcgcgagt gccactgacg ccctcagcga ccagatgctc atcacctttc
     1621 gtgaaggcgc gccagcggac ctcgaagagt attgcttcat tcattgccac ggcgagttga
     1681 aaggtgcact ccatcccggt ttgcaatttt cactcgggca gcatcgctat ccggtgaccg
     1741 ctgttggcag cgtggcggaa gacaaccttc gcgaactggg tcatgtcacc ctgcgcttcg
     1801 atggtttaaa cgaagcggaa tttccgggca ctgtccatgt ggcaggccct gtacccgacg
     1861 atatcgcgcc gggatcggtt ttgaagtttg aatctgttaa ggagtaaaaa atgaatcagg
     1921 ttgccgttgt catcggtggt gggcaaacct taggcgcgtt cctgtgccac ggtctggctg
     1981 ccgaggggta tcgcgtcgcg gttgtcgata ttcagagcga caaagccgca aatgtggcac
     2041 aagaaattaa cgccgaatat ggtgaaggta tggcgtacgg ttttggtgct gacgccacta
     2101 gcgagcaaag cgttctggcg ctctctcgtg gggtagatga aatctttggt cgcgtggatt
     2161 tgctggtcta tagcgccgga atagcaaaag cagcctttat cagcgacttc cagctcggcg
     2221 attttgaccg ttcgctacaa gtgaatctgg tgggttattt cctgtgtgcc cgtgaatttt
     2281 cgcgtttgat gatccgcgac gggattcagg gtcgcattat tcagatcaac tcgaaatccg
     2341 gcaaagtggg cagcaaacac aactctggct acagcgcagc gaaatttggt ggcgtcgggc
     2401 tgactcaatc actggcgctg gatctggcgg agtacggcat tacggtgcat tcactgatgc
     2461 tcggtaacct gctgaaatcg cggatgttcc agtcactgtt gccacaatac gcgaccaagc
     2521 tgggtatcaa accggatcaa gtcgagcagt attacatcga caaagtaccg ctcaaacgcg
     2581 gctgcgatta tcaagatgtg ctgaatatgc tgctgttcta cgccagtcct aaggcgtcgt
     2641 actgcaccgg acagtcgatc aatgtcaccg gcggtcaggt gatgttctga tcaacagcgg
     2701 agatccgtta aggatctccg tgagactata gaatgcctga tgcgctacgc tcatcaggca
     2761 tacaggactt ccgccactac attaaggaaa agttatggta tccgcactca tcaccgtcgc
     2821 cgttatcgcc tggtgtgcgc aactggcctt aggcggctgg caaatttctc gttttaaccg
     2881 tgccttcgac acactatgcc agcaagggcg ggttggcgtg gaccgttcca gcgggcgctt
     2941 taaaccgcgg gtcgtggtcg ccatcgcgct ggacgatcag cagtgcatcg tcgacacctt
     3001 gtttatgaaa ggactgactg tcttcgcccg accgcaaaaa attcccgcaa ttaccggcat
     3061 gcatgcgggt gatttacagc ccgatgtgat ctttccccat gatccactat cacagaatgc
     3121 tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt tgtgaatgtt acttgcttgc
     3181 gaagttatca ttttgaaacc taaatcaggt aatcacgccc atgaaacctc gtcagcgtca
     3241 ggccgccatt ctggagtatc tgcaaaagca gggtaaatgc tcggttgaag aattggcgca
     3301 atactttgac accacaggca caaccattcg caaagatctg gtcattctgg aacatgccgg
     3361 aaccgtcatt cgtacttatg gcggagtggt gttgaataaa gaggaatccg atccgcctat
     3421 cgatcataaa acgctcatca acacccacaa gaaagagctg attgcagaag ctgccgttag
     3481 ttttatccat gatggcgatt cgatcattct tgatgctggc agtaccgttt tgcagattgt
     3541 tcccctgctc tcgcgcttta ataacatcac ggtgatgacc aacagcctgc atatcgtcaa
     3601 tgcgctatcc gaactggata acgaacaaac tatcctgatg ccaggcggaa catttcgcaa
     3661 aaaatcggcc tcatttcacg ggcaactggc agagaatgcc ttcgagcatt tcaccttcga
     3721 taaattgttt atgggcaccg acggcatcga tctcaatgcg ggcgtaacca cgttcaacga
     3781 ggtttatacc gtcagtaagg caatgtgcaa tgccgcgcgc gaagtgattt tgatggcgga
     3841 ctcatcaaag tttggccgta aaagccccaa cgtagtttgc agtcttgaaa gcgtcgataa
     3901 gctaattacc gacgcaggta tcgatccggc gtttcgtcag gcgctggaag agaaagggat
     3961 cgatgtgatc ataaccggag agagcaatga gtgaagcact actgaacacg ggacgtcaga
     4021 cgttaatgct ggagttgcag gaagcaagcc gtttaccgga acgtctgggc gatgattttg
     4081 ttcgcgccgc caatatcatc ctgcactgcg aaggcaaagt ggtggtttcg ggaattggca
     4141 aatcgggcca cattggtaag aaaatcgccg caacacttgc cagtaccggc acaccggctt
     4201 tttttgtcca tccggcagaa gcactgcacg gcgatctggg gatgattgaa agccgcgatg
     4261 tgatgctgtt tatctcttac tccggtggcg cgaaggaact ggatctgatt attccgcgtc
     4321 tggaagataa atctatcgcg ctgctggcga tgaccggcaa accgacgtca ccgctgggcc
     4381 tggcggcgaa agcggtgctg gatatctccg tagaacgcga agcctgcccg atgcaccttg
     4441 cgccgacctc cagcaccgtc aataccctga tgatgggtga cgcgctggcg atggcggtca
     4501 tgcaggcgcg cggttttaat gaagaagatt ttgcccgctc ccacccagct ggggcactgg
     4561 gcgctcgctt gctgaataaa gtgcatcatc tgatgcgtcg tgatgacgct atcccacagg
     4621 tagcgttaac cgccagcgtg atggatgcga tgctggaact cagccgcacc ggtctggggc
     4681 tggtggcggt atgtgacgct caacaacagg tacaaggcgt ctttaccgac ggcgatttac
     4741 gtcgctggct ggtaggcggc ggcgcactca ccacgccagt taatgaagcg atgacgacag
     4801 gcggcaccac attacaggca caaagtcgcg ccatcgacgc caaagaagtt ctgatgaaac
     4861 gcaaaatcac tgccgcacca gtggtagatg aaaacggcaa actcaccggc gcaattaacc
     4921 tgcaggattt ctatcaggcc gggattattt aatccttcaa tcccagacgt ttcgccagcc
     4981 gatgcaggtt ggcgacgtca gtttccagca ttcgcgcaca ggctgcccag ttgtgatgat
     5041 tttgtgccag tgcctggcga atagtttcac gctggaacgc ttctgtcgct tcacgcaggt
     5101 tttgcttaac aacgggcacc gccgccgctt ctggcggcgg caacgtcacc tcaggaaaag
     5161 caaaatgttg cgcctcaaga atcacttcat cgccgttgcg ggtggctctc gccagcacta
     5221 ccgcccgatg aatagcatgt tccagttcgc gcacgtttcc cggaaaactg tagtgttgca
     5281 gtaaatttcg cgccccggca cttaatacca cgcgggagag cccctgccgc aaacgacact
     5341 gctcgcagaa ataccccgcc agcagaatga catcatcgcc ccgctcacgc agcggcggca
     5401 ccgaaagtgg aaacacgctc aggcgatgaa acaaatcggc gcggaatcgc cctgccagca
     5461 cctcttcgcg taagtcgcgg ttagtcgccg ccagcacgcg cacatcgacc cgcaaactac
     5521 ggtcatcgcc aacgcgctga atatcgccat actgcaacac cctcagcagc ttggcctgca
     5581 atgccaacga caactcgccg atctcatcga gaaacagagt accgttatcc gccatttcaa
     5641 acttcccgct gcggttactg atagcgccag taaacgctcc tttcacatgc ccgaacaact
     5701 cactttccgc cacactttcc ggcagtgcgg cacagttgag atagaccagc ggattcaccg
     5761 cccgtggcga ggcttcatga atcgctttcg ccaccagctc cttaccggtt cccgtctcac
     5821 cgctgatcag aacgttgaga tcggacgccg ccacaatctc aatctctttt ttcagttgcg
     5881 tcatgccagg ggacaagcca atcatctgcg tctgtttcac cgcttcaaac ggcgtggcat
     5941 cgcctggcag catattctgg ctttccagtt gttcaatcag caacgcattg cttaacgctc
     6001 ccgccgccag cgcggcaatc agccgtaact cttcgtcgct gaaaacatcg aactgatcgg
     6061 gctgcatccc gtcgagcgtc agtgcgccga tcaggttttg tccgacaaac aatggcagac
     6121 caacgcaggc gtgaaccttc agactctcct gcccaggaat caaaccgtca tagggatcgg
     6181 gcaattcgct gtctgcggga aagcgcacca catccccggc gcgggcaatc gcttccagcc
     6241 gtggatgccc ttccagcgca aagcgtctac cgagtacatc ctttgccaga ccgtcgatgg
     6301 caagcggaat aaactgccgc gaatcgtaac gtagcaacgc agacgcatcg cactccagca
     6361 cctgacgtag cgtggtgatc aggcgctgaa aacgatcctg atgaccaatc ccacgctgca
     6421 attcgatggc gatattcgcc agcacatcaa cggaaaaact catctttgcc tcactgtcaa
     6481 tttgactata gatattgtca tatcgaccat ttgattgata gtcattttga ctactcatta
     6541 atgagcataa ttttatttat agagtaaaaa caatcagata agaaactggc acgcaatctg
     6601 caattagcaa gacatctttt tagaacacgc tgaataaatt gaggttgcta tgtctattgt
     6661 ggtgaaaaat aacattcatt gggtgggtca acgtgactgg gaagtgcgtg attttcacgg
     6721 cacggaatat aaaacgctgc gcggcagcag ctacaacagc tacctcatcc gcgaagaaaa
     6781 aaacgtgctg atcgacaccg tcgaccataa attcagccgc gaatttgtgc agaacctgcg
     6841 taatgaaatc gatctggcgg atatcgatta catcgtgatt aaccatgcag aagaggacca
     6901 cgccggggcg ctgaccgaac tgatggcgca aattcccgat acgccgatct actgtactgc
     6961 caacgctatc gactcgataa atggtcatca ccatcatccg gagtggaatt ttaatgtggt
     7021 gaaaactggc gacacgctgg atatcggcaa cggcaaacag ctcatttttg tcgaaacacc
     7081 aatgctgcac tggccggaca gcatgatgac ttacctgaca ggcgacgcgg tgctgttcag
     7141 taacgatgct ttcggtcagc actactgcga cgagcatctg ttcaacgatg aagtggatca
     7201 gacggagctt ttcgagcagt gccagcgtta ctacgccaat atcctgacgc cgttcagccg
     7261 cctggtaacg ccgaaaatta ccgagatcct gggctttaac ttaccagtcg atatgatagc
     7321 gacctcccac ggcgtggtct ggcgcgataa cccgacgcaa attgtcgagc tgtacctgaa
     7381 gtgggccgct gattatcagg aagacagaat caccattttc tacgacacca tgtcgaataa
     7441 cacccgcatg atggctgacg ctatcgccca gggaattgcg gaaaccgacc cacgcgtggc
     7501 ggtgaaaatt ttcaacgtcg cccgaagcga taaaaacgaa atcctgacca atgtcttccg
     7561 ctcaaaaggc gtgctggtcg gcacttcgac gatgaataac gtgatgatgc cgaaaatcgc
     7621 cgggctggtg gaggagatga ccggattacg cttccgtaac aaacgcgcca gtgctttcgg
     7681 atctcacggc tggagcggcg gtgcgatgga tcgcctttcc acgcgcctgc aggatgcggg
     7741 tttcgaaatg tcgctcagcc tgaaagccaa atggcgacca gaccaggacg ctctggagtt
     7801 atgccgtgaa cacggtcgcg aaatcgcccg tcagtgggcg ctcgcgccgc tgccgcagag
     7861 cacggtgaat acggtagtta aagaagaaac ctctgccacg acgacggctg acctcggccc
     7921 acgaatgcag tgcagcgtct gccagtggat ttacgatccg gcaaaaggcg agccaatgca
     7981 ggacgttgcg ccaggaacgc cgtggagtga agtcccggat aacttcctct gcccggaatg
     8041 ctccctcggc aaagacgtct ttgacgaact agcatcggag gcaaaatgag caacggcatt
     8101 gtgatcatcg gttcgggctt cgccgcccgc caactggtga aaaatattcg caaacaggac
     8161 gccactattc cattaaccct gattgccgcc gacagcatag atgagtacaa caaacccgac
     8221 ctcagccatg ttatcagtca ggggcaacgt gccgacgacc ttacccgcca gacggcgggt
     8281 gaatttgccg ggcagtttaa tctgcgcctg tttccgcaca cctgggtgac ggatatcgat
     8341 gccgaagccc atgtggtgaa aagccagaat aatcagtggc aatacgacaa gttagtgctg
     8401 gcaaccggtg ccagcgcctt tgtcccgcct gtgcctgggc gtgagttaat gctgacgtta
     8461 aatagtcagc aagagtatcg cgcctgtgaa acgcaactgc gggatgcccg acgcgtgttg
     8521 attgtcggcg gtggtttgat tggtagcgaa ctggcgatgg atttttgtcg tgcaggcaaa
     8581 gcggtcacgc taatcgacaa cgctgccagt attctggcgt cattaatgcc accagaagta
     8641 agcagccgct tgcagcatcg gttgacggag atgggcgttc atctgctgtt gaaatctcag
     8701 ttgcaggggc tggaaaaaac ggattctggc attctggcaa cgctggacca ccagcgtagt
     8761 atcgaagtgg atgcggtaat tgccgccacc ggactgcgcc cggaaacagc cctggcacga
     8821 cgcgccgggc tgacgattaa tcgcggcgtt tgcgtcgata gttatctgca aaccagtaat
     8881 gccgatattt acgcgctggg cgattgcgcg gaaattaacg gtcaggtatt gccgttcctg
     8941 cagccgattc aacttagcgg gatggtgctg gcaaaaaatc ttctcggcaa taacacgccg
     9001 ctgaaactac cggcgatgct ggtgaaaatc aaaacgccgg aattaccgct gcatctggca
     9061 ggcgaaaccc agcgtcagga tttacgctgg cagattaata ccgaacgcca gggaatggtg
     9121 gcgcgcggcg ttgacgatgc tgaccagctt cgcgcctttg tggtcagtga ggatcggatg
     9181 aaagaggcat ttgtattgtt gaaaacgttg ccggtgtagg tgggctactg tgcctaaaat
     9241 gtcggatgcg acgctggcgc gtcttatccg acctacgggg acgcatgtgt aggccggata
     9301 aggcatttac gccgcatccg gcaagggtgt ccaaatgcaa cacgctttat ccgttctgga
     9361 cttcacccgc taaccaacgc gccgcagcaa taaccccctg ccccagagac aaaccgccat
     9421 cacccgccgg taaactctgt ggaaagagca atgtgaaatc agcgagataa tgcgccagac
     9481 gtgcacgcag caaacggtta tgaataaccc cgccgctaaa taccagcgta gtgataccac
     9541 gcatcgtggc ctgctcacgc atcaacgcgg caaaaccctg cgccagcgca tcatgaaacg
     9601 cccacgcgcg ttgattaacc agtgcctgcc agttcagcca ctgctgccag aaagttgcga
     9661 gatccagttg attgtccacc cgcggcatcg tcaccggatg cgtcactccg tcgcatgagg
     9721 ctgcgagcgc ctccagagca caagccgctt caccttcata acttaacgtg gctggcgcac
     9781 agcccagtgc cgccgccact gcatcgaaca accgcccaca cgatgatgcc agcggcgcgt
     9841 taattccacg ctcaatggcc cgcgccagta cgctccagtt ttgctgttgc acacttgccg
     9901 tttcagggta attctgccac tccggcacaa agcgcaggca ctgcgccagc aggtttcgcc
     9961 acggctgctt cgccgccaaa tcgccacccg gaagcgccac tgcaggcaag ccgcccaggt
    10021 gctcacattc gcgatagttc acccgcaggc actcgccgcc ccacaaagcg ccattctccc
    10081 ccataccgat accgtcgagc gtcaaagcaa tgacatcacc gccatccagc ggccactgat
    10141 gctctgccag acacgccgct gcatgggcat gatgatgcag taccgtttgc gtcggcagat
    10201 tcatttcgct cgcccactgg ctggagacat agcccggatg cacatcatgc acaatgtatt
    10261 gcggagtgaa atcgtagatg ttttgcatca ggcgtaacgc ttcgcgccac tgcatctgga
    10321 tgccatcgtc acttaaatcg cccagatgct gactcaacac cgcttgttca ccgcgcacca
    10381 ggcagaaggt atttttcaga tccgcgccaa gacacagcac aggcggaaca tttttaaagc
    10441 ccggaggcaa aaccagcgca tccggcacat acccccgcga acggcgcagc atttcgccgc
    10501 tttcgcgcac cactgaatca tccatccgct gcacgatgtc gcggttatgt agcaagaatc
    10561 cgtcggcaat gccctgcaaa tccgccagcg cctgttcgtt gctgatagct ggtggtttac
    10621 cgctcaggtt gccggaggtc atcaccagcg ggcattgcag ttcctgtaac agcaaatgct
    10681 ggagcgggtt cgcaggcaac atcaccccga cttcgttaag gccaggggcg atatcatcac
    10741 aaagctcagg tacgtatttt ttatccacca gcacaatcgg cgcagcgggc gtggtaagca
    10801 actggcgcgc agcgtctggt aaactttctg ccactggcaa catgactgcc agcggtttcg
    10861 ccgggcgatg tttgcgctcc cgcagtgtcg ccaccgcgtt actgttacgt gcatcgcagg
    10921 caagatgaaa tccgccaatc cctttgatgg cgacaatgtt gcccattttt aactgtgcga
    10981 tagctgcctg taatgccgcc tcttgttctg catgttcacc atgacttacc cattcaagat
    11041 gcgggccaca ctccgggcag gccaccggct gggcgtggaa gcgacgatcg agcgggtcgc
    11101 ggtactcttt gtcacaggcc ggacatagcg gaaacgccgc catcacggta aacgggcggt
    11161 cgtaaggcat cgcgcgaata atggtgaaac gcgggccgca gtgggtacag ttgataaacg
    11221 gataacgata acgccgctcg cctggggtat tcatttcggc aaggcaagca gggcaagtgg
    11281 cggcatccgg gacaatttgc gtattcatgg tgccgccggt gctctggcgg atggtgaact
    11341 cggtgggcag ttgtgaccag ataaacggct cacgctcgac gctatcaata cgcgccagtg
    11401 gcggacagtg ctgatgcaat tgaacaagaa acgtttccgg gtcttccagc agccggactt
    11461 ctacgccatc gccgtcatta catacatcgc cgtgaagatt taattgctgt gccagctgcc
    11521 agacaaacgg acgaaaaccg acgccctgca ctttgccacg aatacgcagt tggacaccgc
    11581 aagatgtgtt ttttgccatt gagttattcc cgccatcatg aattgcgtaa cccgccctgc
    11641 cggacacgac agcgtcgtat ccggcagtct caggtcggcg ataccgcccg ctccgtattc
    11701 tacgaatatt tccgggaatt cctttgatgc cagaacagtt ctgtaagatt tttagaacat
    11761 cagcgccgta cggcggcgtt tttctgcgct cagttgttca agtttattac gatcgacaca
    11821 aatcagcgca tgggtcgggc aagccgccat acacgccggg ccgtcttcac gatggttgca
    11881 caggtcgcat ttattggctt cggctttgtc agcccgtaca ttcagacccg cgccgctgtt
    11941 gcgaatcaca ggacgtacca ccacttccat cgcgccatac gggcaagcca ccacgcaggt
    12001 tttgcaacca atgcaacgtt cctgcatcac aggaacaaac cctttatcac ggctgatagc
    12061 accattcggg cagacgttag cgcacggtgc atcttcacac tgacggcaaa ctgtcgccgt
    12121 ggaaatgttc acacctttaa tgacatggat acgcggtaaa aatgtttccg gggtcagcga
    12181 tgcacagtcc tgattttcct gatgagaaac cacgcacgcc acttcacagg tacggcaacc
    12241 aatacattta ctcgcgtcag caatgatgaa acggttcatc aaattctcca gcaatgacag
    12301 ttaatgcgcc gatacactca caaatcatgc cagtttttaa tttgctgtta tttaaggaaa
    12361 ttaatttctg taatgcagga aaaaagatgt catcgacact agtgacgatg acatgtgatg
    12421 acaatgttta tcgcgaaagg gcaatgagtg agtcgcggcg gatcagtttt ccgctaaagg
    12481 ttttcggcgg tgagaaatcc ccgccatcga gcataaaaat cagccgtcca ataatttcct
    12541 gaatcatctc agtcaccgga atttttacgc tggagagcgc cggaacgata tagggggcaa
    12601 ttgcgatatc atcgaatccg ataactgaca cctgctctgg caccgctacg ccgcgctcgt
    12661 gcaacgcttt gatcgcacct atcgccatat cgtcgttact ggcaactaac gcgctaaatt
    12721 tagcccctcg ttcgagcaac gtttctaccc cttcggcccc gctggcgggc gtccatttac
    12781 cgttagcgat aagtttttca ttgagcgcaa taccatgctg cgacagcgcg tctttatacc
    12841 cggcaagacg ttcaatgctg gtgggggaat ccatcgagcc ggtaaggaaa gcaatctcct
    12901 gatgcccggc gtttatcaac tctgccacgg cgttaaagct ggtctgttta tgatcgcacc
    12961 agacgctatg gctgctatta ttgcgcaggc ggcgattaag caccattatc ggctgactgt
    13021 gcgcgtcaat gatgtcatca atctcatcca cgcttaaaaa acgcgggtaa atcataatgg
    13081 cgtcgcagcg caaatccagc agatactgaa tcgcctggcg ctcttcttcg gcgctgtgtt
    13141 taccatctgc caatagcaac tgtcgccctt tctcttccgc cattcgcgcg gcatgaaaca
    13201 gtaattcact aaaataaatg ccgtgataaa gcgtgttggt cactaccagc cccagcgtct
    13261 gagtactctt cgccgacaga ttgcgggcca gcaaatttgg gcggtaactg ctctcttcta
    13321 ccgcctgaaa cacgcgatct ttagtctcct ggctgacgta gccattacct gaaagcacgc
    13381 gggaaacggt cgcttttgaa accccggcgc gcttcgccac ttccagcatc gtcgtcatca
    13441 ttttcatccc tttacacgca atcaacgcag tgtactgcac cgtttgccga ttgtccttgc
    13501 gcaatcggct ccagcggata agacgaggtc gccgtgccgg ttttgatgac ttttttgata
    13561 aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt tacgctcaat gctgtaacgc
    13621 tcgagttctt tgtacggcac cactttgctc ttcttcttac gcacatcgac tacggtcatg
    13681 cggtcggtac aggagtagca agggtccagg ctaccgataa tcagcggcgc ataggaaacg
    13741 gtattgccac gcagcatgta gcgcagggtc ggccagttcg cgtaggtcgc ggcacggcag
    13801 cgccagcggt acagcttctg gttgtcgccg gtcatgctcc agtggatatc gtcgccgcgc
    13861 ggcgcttcgg caaagcccag cgcgaaacgg tgcggaatgt aggtaaagcc ttccaccatc
    13921 agcggaccgc ccggcaggtt atccagaccg tagtcgatca tattcagcgc ggtatagacc
    13981 tcgttgattc gcactttcag acgggaaata acgtcgcagc cctgctcgct gtggacttcc
    14041 attggcagca ggccataacc gacaaacggg tgatcggcgc gagtatcgcg ggcgagaccg
    14101 ctggcgcgaa ccatcgggcc aacgttactg aaatcgcggg cgatttccgg gtccagacga
    14161 ccaatgccga cggtgcgttg ttccatgttc ggcgtactca gcagcacatc caccagctcc
    14221 tgcacttcac gacgcatttg ttgcgccagc tggcgggtct ggatcatgtc atctttcagc
    14281 agatcgcgac gaatcccgcc gatcaggttc aggccgtagg ttttacgcgc accggtaagg
    14341 atctctgcca ttttcatgga ggtttcacgc acgcggaaga actgcataaa gccggagtcg
    14401 aagccggtaa agtgacaggc gaggccgagg ttgagcagat gcgggtgcag gcgttccacc
    14461 tccagcagaa tggcgcggat catctgcgcg cgttctggca cctgaatacc catcgcgttt
    14521 tccaccgacg tggtgtaggc ggtgctgtgg gcaaagccgc agatcccgca cacacggtca
    14581 gagaggaagg tcacttcgtt ataacccatg cgggtttccg ccagtttttc cataccgcga
    14641 tggacgtaga acagacggta gtcggcgtcg ataatgtttt cgccatcgac gaacagacgg
    14701 aagtgacccg gttcgtcaga agtgacgtgc agcggaccaa tcggcacgac gttgtttttc
    14761 ttgtcgccca gttcgttgat gaactcgtag gtttcagcat cggtagtcgg tgccggacgc
    14821 tgacgataat ccatgctgtc tttacgcagc ggataaagtt catccggcca gtcatccggc
    14881 agcaccagac gacgttcatc cggcagacca accggaatca aaccgtacat atcgcgcact
    14941 tcgcgctcgc cccacaccgc cgccggaacg cgcggcgtca cggacggata ctccggtttg
    15001 ttggcgtcaa cttcgacgcg aaccgttacc caacacttgg tgcccttctc catcgacagc
    15061 acgtaataaa cggcgtaatg accattcagt ttgcgttcgt cgttaccaaa cagcaccgac
    15121 agccagccac cctgcttgta gtagagaaac tccaccactt ccggcagata gttcaccttt
    15181 acggtgacag tcagctgatc tttggtctgc caggcgtggt ccagcacgac gcctagaaat
    15241 gcctcattca gcgcggcgag ataatgttga cctaattttt cttcagacat gctcaaactc
    15301 tctttaatca cgccgccagc aaggagacga acgctaaaaa tgcaaagcca aacccagccc
    15361 aggtaatgcg cggagtaata tcaagacgca gacgggccat gctgttttcg aacagcgcga
    15421 taaccaggac gccgaccacc agtttgacga tggcaatcac cagcgccagc agcagtccac
    15481 cgacggtgaa ggtttccatt tgtccccacg gaataaacac cccgacgaac atctgcaaca
    15541 ccaccagctg tttcaggctg ataccccatt tcatgacgcc aaagccgcta ccgctgtatt
    15601 cggagagcgg accttcctgc aactcctgct cggcttccgc caggtcgaac ggcagtttgc
    15661 ccatttcgat aaaagtggcg aacgcacagg cgcagagcgc cagcaccagc gggatgcttt
    15721 ggctcagcgg ccagtgataa acggtgtcgg taatgttgct gatgttggtg gaaccggcaa
    15781 cctgtgcggc aacccacaga ccaagcagca acatcggttc gaccagcacg ccaagcatcg
    15841 cttcacggct cgcaccgata gcggtaaacg ggctaccggt atccagaccg gaaatggcaa
    15901 agaagaaacg cgcgatggcg aagagataca gtaaggtgat caaatcgccc agttgcggca
    15961 gcggagaacc gacggtcacc accggcagcg cagtagcgat agtcagcatg acgcccacca
    16021 tcacatacgg cgtcaggcgg aacatccagc cagaggcatc cgggccaacg ctctggcggc
    16081 ccagcagttt gataatgtcg cgatactcct gcaacacgcc cggcccgcga cggttatgca
    16141 gacgggcgcg cgccacgcgg gtaataccgg agagcagcgg cgcaacggca aataacacca
    16201 gcgcctgaat taacggatat aaaacactca ttctcaggct cctcgtgaaa caataatcac
    16261 caccagtacc gccagttcaa ccagcgccat ccggcggaac agcaacgcac taccttcgca
    16321 ctgccagccc ggcactagcg acaccggatt cagccatttg cgcagtttca gcactggcgc
    16381 aaacgcctgt ttaaccggca tggcaaaacc gtgagcggta atcaccattg atttttcatg
    16441 gtcgtagccg cacacccagg ccgcaccgcg ggaacgtgac ggcaggcggt cgcctttgca
    16501 aatcgccata atgatgaatg gcagcagcgg gcaggcaatc agcagcaatg tgatcattgg
    16561 ttgagaaacg gtggtgttag caggctccag aggcagaggt acagcagcag agagcatcgg
    16621 cagcagccac ggcgcagcaa caccgccaat tacgcagcaa atcgccagtg ccactacgct
    16681 tacgctcatc aggagcggcg cacaggtggc gttttcggct tctttggtac gcggcgcgcc
    16741 gaggaacgtg acgccataga ctttcgccat acacatcacc gccagcgcac cggtaattgc
    16801 cagacccaca gcgagcagcg gccccagcag acgggcaaca aacgcgccgc tattgctcag
    16861 tttgaagaag gattgataga taacccattc cccggcaaac ccgttcagtg gcggcagcgc
    16921 agccattgcc atcaacccga ctaacatggc gatggagata accggcattt tcttgccaat
    16981 accaccgagt ttttcgatat cgcgatgacc ggtacggaac cagacgctcc ccgccccgag
    17041 gaacagtacg cttttgaaca ggctatggtt aagcagatgg tacagaccac cgaccaggcc
    17101 aagagcaatc agcgccggtt gttcgagcgc gataccc
//
LOCUS       DAZPEU010000073        17126 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPEU010000073 DAZPEU010000000
VERSION     DAZPEU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.073"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            120..1259
                     /gene="arnB"
                     /locus_tag="ACHU2L_002179"
     CDS             120..1259
                     /gene="arnB"
                     /locus_tag="ACHU2L_002179"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR1357027.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1263..2231
                     /gene="arnC"
                     /locus_tag="ACHU2L_002180"
     CDS             1263..2231
                     /gene="arnC"
                     /locus_tag="ACHU2L_002180"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR1357028.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2231..4212
                     /gene="arnA"
                     /locus_tag="ACHU2L_002181"
                     /pseudo
     CDS             2231..4212
                     /gene="arnA"
                     /locus_tag="ACHU2L_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            4209..5099
                     /gene="arnD"
                     /locus_tag="ACHU2L_002182"
     CDS             4209..5099
                     /gene="arnD"
                     /locus_tag="ACHU2L_002182"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR1357029.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVECCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5099..6751
                     /gene="arnT"
                     /locus_tag="ACHU2L_002183"
     CDS             5099..6751
                     /gene="arnT"
                     /locus_tag="ACHU2L_002183"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR1357030.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            6748..7083
                     /gene="arnE"
                     /locus_tag="ACHU2L_002184"
     CDS             6748..7083
                     /gene="arnE"
                     /locus_tag="ACHU2L_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR1357031.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7083..7469
                     /gene="arnF"
                     /locus_tag="ACHU2L_002185"
     CDS             7083..7469
                     /gene="arnF"
                     /locus_tag="ACHU2L_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR1357032.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7463..7735)
                     /gene="pmrD"
                     /locus_tag="ACHU2L_002186"
     CDS             complement(7463..7735)
                     /gene="pmrD"
                     /locus_tag="ACHU2L_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR1357033.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7840..9195)
                     /gene="menE"
                     /locus_tag="ACHU2L_002187"
     CDS             complement(7840..9195)
                     /gene="menE"
                     /locus_tag="ACHU2L_002187"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR1357034.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9192..10154)
                     /gene="menC"
                     /locus_tag="ACHU2L_002188"
     CDS             complement(9192..10154)
                     /gene="menC"
                     /locus_tag="ACHU2L_002188"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR1357035.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(10154..11011)
                     /gene="menB"
                     /locus_tag="ACHU2L_002189"
     CDS             complement(10154..11011)
                     /gene="menB"
                     /locus_tag="ACHU2L_002189"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR1357036.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11026..11784)
                     /gene="menH"
                     /locus_tag="ACHU2L_002190"
     CDS             complement(11026..11784)
                     /gene="menH"
                     /locus_tag="ACHU2L_002190"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR1357037.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11781..13451)
                     /gene="menD"
                     /locus_tag="ACHU2L_002191"
     CDS             complement(11781..13451)
                     /gene="menD"
                     /locus_tag="ACHU2L_002191"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR1357038.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13540..14835)
                     /gene="menF"
                     /locus_tag="ACHU2L_002192"
     CDS             complement(13540..14835)
                     /gene="menF"
                     /locus_tag="ACHU2L_002192"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR1357039.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14914..15219)
                     /gene="elaB"
                     /locus_tag="ACHU2L_002193"
     CDS             complement(14914..15219)
                     /gene="elaB"
                     /locus_tag="ACHU2L_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR1357040.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15274..15735)
                     /locus_tag="ACHU2L_002194"
     CDS             complement(15274..15735)
                     /locus_tag="ACHU2L_002194"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1357041.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15800..16717
                     /gene="rbn"
                     /locus_tag="ACHU2L_002195"
     CDS             15800..16717
                     /gene="rbn"
                     /locus_tag="ACHU2L_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR1357042.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16908..17096
                     /locus_tag="ACHU2L_002196"
                     /pseudo
     CDS             16908..17096
                     /locus_tag="ACHU2L_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         3993 a         4631 c         4348 g         4154 t
ORIGIN      
        1 gtttcttaag gttaagttaa tattctatcc ttaaaatttc gctccaaatg gcaaaatata
       61 cacaacactc tttatagcaa atataagtgg acaggtattc aatggcggaa ggaaaagcaa
      121 tgtcagaatt tttgcctttt tcgcgaccag caatgggcgt ggaggaactc gctgcagtta
      181 aagaggttct cgaatccggt tggatcacaa ccggtccgaa gaatcaggcg cttgagcaag
      241 ccttttgcca gttgacggga aatcagcatg ccatcgcggt cagttcagcc accgccggaa
      301 tgcatatcac gctaatggcg ttggaaattg gcaagggcga tgaagtgatt acgccttccc
      361 tgacctgggt ttcaaccctc aatatgattt ccttgctggg tgcaacgccg gtaatggtgg
      421 atgtcgaccg cgatacgctg atggtcacgc ctgaagcgat cgaatcagcc attacgccac
      481 gcactaaagc catcattccg gtgcattatg ccggtgcgcc agcagatatt gacgccattc
      541 gcgccattgg cgaacgttac ggcatcgcag ttatcgaaga tgctgcccat gccgtcggta
      601 cgtattacaa agggcgacat attggcgcaa aaggtaccgc tattttttca tttcatgcca
      661 ttaaaaatat tacctgtgct gaaggtggcc tgattgtaac tgataatgaa aaccttgccc
      721 gccagctacg gatgctgaaa tttcacggtc tgggtgtcga tgcctatgac agacaaacct
      781 ggggccgtgc accgcaggct gaagtcttaa caccgggcta taagtacaat ctgaccgata
      841 ttaacgccgc gattgccctg acacagttag tcaaattaga gcacctcaac acccgtcggc
      901 gcgaaattgc ccagcaatat cagcaagcac tggcagctct cccctttcag ccattaagcc
      961 ttcccgcctg gccgcacgtt cacgcctggc atctgtttat tattcgtgtc gatgaacaac
     1021 gttgtggtat cagtcgcgat gcgttgatgg aagcgttaaa agaaagaggc attggtaccg
     1081 ggttacattt ccgcgccgct cacacacaaa aatattatcg cgagcgtttt cccacgctgt
     1141 cgttaccgaa taccgaatgg aatagcgaac gcatctgttc gttgccgctg ttcccggata
     1201 tgactaccgc cgatgccgac cgcgtcatca cagcccttca gcaactcgca ggacaataag
     1261 ccatgtttga aatccaccct gttaagaaag tctcggtggt tattcccgtt tataacgagc
     1321 aggaaagctt accggaatta atcaggcgca ccaccacagc ctgtgaatcg ttggggaaag
     1381 agtatgagat cctgctgatt gatgacggca gtagcgataa ttccgcgcat atgctggtcg
     1441 aagcctcaca agcggagaac agccatattg tgtctatttt gattaaccgc aattacgggc
     1501 aacattcagc gattatggcg ggattcagtc acgttactgg cgacttaatt attacccttg
     1561 atgccgatct ccagaatccg ccagaagaaa tcccccgcct ggtggcaaaa gccgatgaag
     1621 gttacgacgt ggtagggact gtacgccaga accgccagga cagctggttt cgtaaaacag
     1681 cttcgaagat gattaaccgg cttattcagc gcaccactgg caaagcgatg ggtgactacg
     1741 gttgtatgct gcgcgcctat cgccgtcata ttgtcgatgc gatgttgcac tgccatgaac
     1801 gcagcacctt tatcccgatt ctggcgaata tcttcgcccg ccgtgcaatt gaaattccag
     1861 tacatcatgc cgagcgtgag tttggtgaat ccaaatacag ttttatgcgc ctgattaatt
     1921 tgatgtacga cctggtgacc tgccttacca caacgccgct acgtatgctg agtctgctcg
     1981 gcagcattat tgcgattgga ggttttagca ttgcggtgct gctggtgatt ttacgcctga
     2041 ccttcggacc acaatgggcg gcagaaggcg tctttatgct atttgccgtg ctgtttactt
     2101 ttattggcgc tcagtttatc ggcatgggat tactcggtga atatatcggc aggatctaca
     2161 ccgatgtccg cgcccgcccc cgctattttg ttcagcaagt tatccgtcca tccagcaagg
     2221 aaaatgaata atgaaaaccg tcgtttttgc ctaccacgat atgggatgcc tcggtattga
     2281 agctctgctg gctgccggtt acgaaattag cgccattttt acccataccg ataatcccgg
     2341 tgaaaaagcc ttttatggtt cggtggctca tctggcggcg gaaagagaca ttccggttta
     2401 tgcgccggat aacgttaatc atccgctgtg ggtggaacgc attgcccaac tgtcaccaga
     2461 ggtgattttc tctttttatt atcgccatct tatttgcgac gagatttttc agctcgctcc
     2521 cgcaggtgca tttaatctgc acggttcgct gttaccaaaa tatcgtggtc gcgcgccgct
     2581 gaactgggtg ctggtgaacg gtgaaacgga aacgggcgtt acattgcacc gaatggtaaa
     2641 cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc
     2701 gctattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta
     2761 cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt
     2821 tttggtcgca gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg
     2881 cacaacatgg tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat
     2941 cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg
     3001 agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc
     3061 accggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc
     3121 ctggtgcaag gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg
     3181 gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc
     3241 gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg
     3301 aatcatccac attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag
     3361 tatcacgtca aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa
     3421 tatacccgca acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc
     3481 cgctactgcg ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg
     3541 atgtgtagcg ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat
     3601 aaaccacgct ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat
     3661 ggcgaaaaag agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga
     3721 ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc
     3781 aatctggtag aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt
     3841 actgatatcc gggacggtat cgaggcgttg taccacatta tcgaaaatgc gggaaatcgc
     3901 tgcgacggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg
     3961 ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt
     4021 gcgggctttc gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag
     4081 catcgtaaac cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat
     4141 atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat
     4201 aaaccatcat gaccaaagta ggcttacgca ttgatgtcga tgcctttcgt ggcacccgtg
     4261 aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt
     4321 tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt
     4381 tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag
     4441 gtacggcctg gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg
     4501 ctaaacatca cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg
     4561 gtaactggga tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag
     4621 agattatcgg tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg
     4681 tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac
     4741 cgttccgtcc actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac
     4801 ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac
     4861 tcaaccgcat cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaat
     4921 gctgcgctta tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga
     4981 cattttgccc tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg
     5041 gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat
     5101 gaaatcggta cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc
     5161 gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga
     5221 aatgctggca tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa
     5281 acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt
     5341 tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt
     5401 tactctgcgc ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc
     5461 attgtttatt gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg
     5521 gctggtggcg ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa
     5581 aagcgcagga tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt
     5641 tctcgccctt gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg
     5701 gaaagatctc tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc
     5761 ctggggactg gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga
     5821 gcatattcaa cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt
     5881 gccggtcatc attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac
     5941 aggctggaaa aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc
     6001 gctgctgttt ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc
     6061 acctctggca atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc
     6121 gctgcggatt aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt
     6181 tgtgatctcc ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa
     6241 agtcttttgt gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac
     6301 aaacgtcgaa aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt
     6361 aggattttca attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat
     6421 gacacaagaa tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc
     6481 aggtctggca tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt
     6541 gaaatacggc cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc
     6601 aaactggctt aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg
     6661 cgatgaagat atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg
     6721 tctggtgctg attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg
     6781 cttagcgttg ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa
     6841 cggcgcaaac atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg
     6901 atgctctggc tgctggtctt gcagaacgta ccggtaggca ttgcctaccc gatgttaagt
     6961 ctgaattttg tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg
     7021 cgtcactggt gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg
     7081 taatgtgcct gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc
     7141 tgggttttgc ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc
     7201 tgttagcttt tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt
     7261 ccgttttttg ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc
     7321 tcagtatgag ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga
     7381 ctttttcgct gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt
     7441 tcctgcccac gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc
     7501 actcatcggg gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat
     7561 taatacaata aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt
     7621 cactcttcac ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat
     7681 gtctgttatc ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc
     7741 tgtttgctaa gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc
     7801 aatggaatgg cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca
     7861 ctcttttagc gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt
     7921 cagccagcgc accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc
     7981 aacggtttgc tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc
     8041 aacggaaacg ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc
     8101 ttccggctga ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt
     8161 cagcttgcca ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac
     8221 cagtgaaacc agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca
     8281 cacttcatta ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag
     8341 gccgtcggct tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa
     8401 gcaacgaatc ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc
     8461 aagtaacacc gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt
     8521 tggcaccagt gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg
     8581 caccgtcatc cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg
     8641 aaacagcggt aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac
     8701 accttgcgca ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt
     8761 agaacccgag gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac
     8821 gccttcaacc agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg
     8881 caccagcgca aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag
     8941 ctgagggttc acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag
     9001 cgtttgcggc gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc
     9061 cgcaaagccc gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc
     9121 gtcattaaga cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca
     9181 gtcagagaag atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc
     9241 cccggccagc gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg
     9301 gtgtctggcg ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca
     9361 atggaagaac tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt
     9421 actttttcca gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca
     9481 gccacaaagg caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt
     9541 tcacgggcaa acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg
     9601 cggtggcgat aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag
     9661 gcgcgatttg cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc
     9721 accatgccgt cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc
     9781 ggcatatcgg caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca
     9841 cggtagttgg ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca
     9901 aaggccacgg aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag
     9961 gcaagcagca cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag
    10021 atctcccccc acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt
    10081 tttaaccgcc tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc
    10141 tgcgcgctac gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt
    10201 tgaaagcgtt gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc
    10261 ccgccagctc ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc
    10321 agcgcagcgc catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt
    10381 ccagatccgc cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt
    10441 cgtactgacg gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca
    10501 tgtaggaagc gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga
    10561 tggcattatc tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga
    10621 tggagtagcc agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga
    10681 agtccagcac gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac
    10741 gcactttctg gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca
    10801 cgccgatatt gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca
    10861 gaggacggaa ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg
    10921 tggatttttc ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg
    10981 cgtaaggcat tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat
    11041 ctgcgccaga cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg
    11101 aggaatgaca tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg
    11161 ttcaccacat aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg
    11221 ctggacggcg agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct
    11281 gcgcagcgcc accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg
    11341 ataccagtcg gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg
    11401 atcggaacgc tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc
    11461 gacaataacc ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc
    11521 gccaagcgag taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg
    11581 cagtaagtcg gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc
    11641 taggagatca acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc
    11701 gtggcaatcg ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg
    11761 ttttgcctgc gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt
    11821 tgcgcaccat cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag
    11881 gcgtcggcaa atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg
    11941 aacatcgcgg cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc
    12001 tcgctttgcg gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt
    12061 aataccagcg gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg
    12121 gagagatcgc ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg
    12181 gtcgaaagca gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc
    12241 ggaagttgcg aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc
    12301 ccctgttcag gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca
    12361 cggcgggcaa taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag
    12421 ggctggcgtt tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga
    12481 cgtccgcgat ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct
    12541 tctggttcac agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt
    12601 tgcaccacaa tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga
    12661 tcggcacacg gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca
    12721 agagtttgcg cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc
    12781 accacgccgc gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga
    12841 ggcgcttcac gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag
    12901 ctaagcccgg tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg
    12961 actccccccg catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg
    13021 gggatatcct gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt
    13081 cccggctggc gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg
    13141 gttaagagaa tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt
    13201 gccaccgccg tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc
    13261 cccagcgcca gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa
    13321 ttctccgccg ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt
    13381 ctgacgccgt gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg
    13441 cttactgaca tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa
    13501 tagattattg atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt
    13561 acgcagccct gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg
    13621 gacaatgccc gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag
    13681 ggaaacgcag aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc
    13741 tcgggtgaac ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc
    13801 aactgctgcc gttggcagca actgatgtaa acagatcaca tcatccgctt tgttgagtga
    13861 agtccagata cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa
    13921 aacatccagc gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat
    13981 gttctcgcgc tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt
    14041 atcatcagga tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg
    14101 ccgccgccat aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat
    14161 gtaaaaatgg tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt
    14221 gaccggactt gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc
    14281 ttcggcgatg gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca
    14341 gtgttgttct cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt
    14401 ggcgataaat tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt
    14461 cagccgcagc gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt
    14521 gccctgcgac gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg
    14581 ctggcgaaga aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac
    14641 ggcagcttct tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact
    14701 ggccagccag ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg
    14761 aatgccgggt gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt
    14821 agtaagtgat tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac
    14881 aaaaaaaatg cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag
    14941 taccagcccg acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc
    15001 atcagcacga taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac
    15061 ccgttttttc acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg
    15121 atcggcggga tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag
    15181 gtcgtcatcg atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa
    15241 aacctgtcac taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat
    15301 cccaatgtgt gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact
    15361 ctggtagaag ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg
    15421 atgcgtacat gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa
    15481 cgcttcactg acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag
    15541 aatcctcgca tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc
    15601 cgtcaaatca tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc
    15661 gcaacgcagt tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc
    15721 ttgccattca atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt
    15781 tttgaaacgt gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac
    15841 ccgcacgcgc aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact
    15901 ttggttgttt gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc
    15961 tggaaaactg gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc
    16021 cggcttgctg tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc
    16081 gcaaggtatc cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta
    16141 tccgctggaa attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt
    16201 aaccgcttat ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga
    16261 taaaccgggt gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact
    16321 gtttcaggaa ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg
    16381 cgcagattac ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg
    16441 cccctgcgat gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac
    16501 gctggatata accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc
    16561 tgcgacacta gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg
    16621 ctatgatgac aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac
    16681 tgaactggcg aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg
    16741 gtgaaacttg ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg
    16801 agcgattttt gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc
    16861 ttttcatctt gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg
    16921 tcagcaatta ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta
    16981 ctgtgaccga ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa
    17041 gtattgagtt actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt
    17101 aatgctgcca acttactgat ttagtg
//
LOCUS       DAZPEU010000074        16853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPEU010000074 DAZPEU010000000
VERSION     DAZPEU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.074"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..4638
                     /locus_tag="ACHU2L_002197"
                     /pseudo
     CDS             1..4638
                     /locus_tag="ACHU2L_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5006..6661
                     /gene="lldP"
                     /locus_tag="ACHU2L_002198"
     CDS             5006..6661
                     /gene="lldP"
                     /locus_tag="ACHU2L_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR1357043.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6661..7437
                     /gene="lldR"
                     /locus_tag="ACHU2L_002199"
     CDS             6661..7437
                     /gene="lldR"
                     /locus_tag="ACHU2L_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR1357044.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7434..8624
                     /gene="lldD"
                     /locus_tag="ACHU2L_002200"
     CDS             7434..8624
                     /gene="lldD"
                     /locus_tag="ACHU2L_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR1357045.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8820..9293
                     /gene="trmL"
                     /locus_tag="ACHU2L_002201"
     CDS             8820..9293
                     /gene="trmL"
                     /locus_tag="ACHU2L_002201"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR1357046.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9346..10167)
                     /gene="cysE"
                     /locus_tag="ACHU2L_002202"
     CDS             complement(9346..10167)
                     /gene="cysE"
                     /locus_tag="ACHU2L_002202"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR1357047.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGCGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10247..11266)
                     /gene="gpsA"
                     /locus_tag="ACHU2L_002203"
     CDS             complement(10247..11266)
                     /gene="gpsA"
                     /locus_tag="ACHU2L_002203"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR1357048.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11266..11733)
                     /gene="secB"
                     /locus_tag="ACHU2L_002204"
     CDS             complement(11266..11733)
                     /gene="secB"
                     /locus_tag="ACHU2L_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR1357049.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11796..12047)
                     /gene="grxC"
                     /locus_tag="ACHU2L_002205"
     CDS             complement(11796..12047)
                     /gene="grxC"
                     /locus_tag="ACHU2L_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR1357050.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12189..12620)
                     /locus_tag="ACHU2L_002206"
     CDS             complement(12189..12620)
                     /locus_tag="ACHU2L_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR1357051.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12865..14409
                     /gene="gpmM"
                     /locus_tag="ACHU2L_002207"
                     /gene_synonym="pgmI"
     CDS             12865..14409
                     /gene="gpmM"
                     /locus_tag="ACHU2L_002207"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR1357052.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14443..15702
                     /gene="envC"
                     /locus_tag="ACHU2L_002208"
     CDS             14443..15702
                     /gene="envC"
                     /locus_tag="ACHU2L_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR1357053.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15706..16665
                     /locus_tag="ACHU2L_002209"
     CDS             15706..16665
                     /locus_tag="ACHU2L_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR1357054.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(16652..16853)
                     /locus_tag="ACHU2L_002210"
                     /pseudo
     CDS             complement(16652..16853)
                     /locus_tag="ACHU2L_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4110 a         4444 c         4485 g         3814 t
ORIGIN      
        1 ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga
       61 aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt
      121 ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac ggtagtggat
      181 cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact tttatgaaca
      241 ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa tatagctctg
      301 ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa
      361 tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg ggccaatact
      421 caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct atgggggact
      481 cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta gctagcggta
      541 ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct attgcgatag
      601 gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg aaggcaaaag
      661 ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat actatcgcat
      721 taggaaatgg cagccaggct ctggcagata atgcaatcgc cataggccag ggcaacaaag
      781 ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc ttaaacacca
      841 ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt ggtagtaata
      901 gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg gatttagaca
      961 ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg aaaaatggcg
     1021 cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc attagcgact
     1081 cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg tactgttaca
     1141 gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc gctcgctgta
     1201 cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc tgctcatggt
     1261 actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat ttcagcctcc
     1321 agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt cgaagccaac
     1381 accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga tgccctgctc
     1441 tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac cagcaaaatc
     1501 accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa cggctctcag
     1561 ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa taacacttcc
     1621 aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac taatcttggt
     1681 gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca tggcaccgag
     1741 accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag caccgatgcc
     1801 gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac caccaatatc
     1861 gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct tggtgaggat
     1921 gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa caataccgcc
     1981 agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga tgccattaac
     2041 ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg caatgcttct
     2101 gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac aaattattat
     2161 aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct cggcgatgct
     2221 ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa tggtgacgca
     2281 agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga cgcagtaaac
     2341 ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg tggtgccgaa
     2401 gtcaatgcag acggcaccat cactgcgccg acgtacacca ttgctaatgc tgattacgat
     2461 aatgtcggtg atgccctgaa tgctatcgat accactcctg acgacgctct gctctgggat
     2521 gcggacgccg gtgaaaatgg tgcatttagc gccgctcacg gaaaagataa aactgccagt
     2581 gtaatcacta acgtcgctaa cggtgcaatc tctgctgcca gcagcgacgc gattaacggc
     2641 tcacaactct ataccaccaa taagtacatc gctgatgcgc tgggtggtga cgcagaagtc
     2701 aacgctgacg gcaccatcac cgcaccgact tacaccattg cgaacgccga gtacaacaac
     2761 gtcggtgacg ccctggatgc gcttgatgat aacgccctgc tgtgggataa gactgccaat
     2821 ggcggtgctg gagcctacaa tgccagccat gacggtaaag ccagcatcat cactaatgtc
     2881 gctaatggca gtattagtga ggacagtacc gatgcagtga acggttctca gttgaatgcg
     2941 acgaatatga tgattgagca gaacacccaa attatcaatc agctcgctgg taacaccgac
     3001 gcaacctata tcgaagaaaa tggcgctggt atcaactacg ttcgtactaa cgacaacgat
     3061 ttagccttta acgatgcaag cgcctctggt gtcggcgcta cagctgtagg ttataacgct
     3121 gtcgcgtctg gtgccagcag cgtagccatt ggtcagaaca gcagcagcac cgttgatacc
     3181 ggtattgcgc tgggtagcag ctccgtttcc agccgtgtga ttgccaaagg ttctcgtgac
     3241 actagcgtaa cggaaaatgg cgtggtgatt ggttatgaca ccactgacgg cgaactgcta
     3301 ggtgcattgt caattggtga tgacggtaaa taccgccaaa tcatcaacgt agctgatggt
     3361 tcagaagccc atgacgccgt tacggttcgc cagttgcaga acgctattgg agcggtcgcc
     3421 actacgccaa ccaagtactt tcacgccaac tcaacggcag aagactcact ggccgttggt
     3481 gaagactcgc tggcaatggg tgcgaaaact gtcgttaatg gtaatgcagg tattggtatc
     3541 ggtttgaaca ctctggttct ggctgatgcg atcaacggca ttgctatcgg cagtaacgca
     3601 cgcgcaaacc atgcaaacag tatcgcaatg ggtaacggtt ctcagaccac ccgtggtgca
     3661 cagactggct acaccgccta caacatggac gcaccgcaga actctgtagg tgagttttct
     3721 gtcggtagtg aagacggtca acgtcagatc acaaacgtcg cagctggttc agcggatacc
     3781 gatgcggtta acgtgggtca gttgaaagtc actgatgagc gcgtagcgca aaatacccag
     3841 agcattacta acctgaacaa tcaggtcact aatctggata ctcgcgttac taatatcgaa
     3901 aacggtattg gcgacattgt caccaccggt agcaccaagt acttcaagac caacaccgat
     3961 ggcgtagatg ccaacgccca gggtaaagat agcgttgcta ttggttctgg ttccattgct
     4021 gccgctgaca acagcgtcgc actgggtacc ggttccgttg cagaggaaga aaatacaatc
     4081 tctgtaggtt cttctactaa ccaacgccgt atcaccaacg tagctgccag tgttaatgcc
     4141 accgatgcgg ttaacgtgtc gcagctgaaa tcttctgaag caggcggagt gcgctacgac
     4201 accaaagctg atggttctat cgactatagc aatatcaccc tcggtggtgg caacggcagt
     4261 acgactcgta tcagcaacgt ctccgctggc gtcaacaaca acgacgcggt gaactacgcg
     4321 cagttgaagc aaagcgcgca ggaaacgaag caatacaccg atcagcggat ggttgagatg
     4381 gataacaaac tgtctaaaac tgaaagcaag ttgagcggtg gtatcgcttc tgcaatggca
     4441 atgaccggtc tgccgcaggc ttatacaccg ggtgccagca tggcttctat tggtggcggt
     4501 acttacaacg gtgaatcggc agttgcttta ggtgtatcga tggtgagcgc caatggtcgt
     4561 tgggtctaca aattacaagg tagtaccaat agccagggtg aatactccgc cgcactcggt
     4621 gccggtattc agtggtaatg atccattaag ttagtgtgac taagggcaaa cgccaaattg
     4681 cctgatgcgt tacgcgcatc aggcaagata aggcgctccg ggtcaacaat ctaagggcaa
     4741 ttctctgatg aggattgccc ttttctttac cagacatctc cccccacaag aattggccct
     4801 accaattctt cgcttatctg acctctggtt cacaattccc caattaaaac tcacatcaat
     4861 attgccaata cataacattt agttaaccat tcattgtcat tatccctaca caacacaatt
     4921 ggcagtgcca cttttacaca acgtgtgaca aggagatgag caacagactc attacacgat
     4981 gtgcgtggac tctaggagac ctgcaatgaa tctctggcaa caaaactacg atcccgccgg
     5041 gaatatctgg ctttccagtc tgatagcatc gcttcccatc ctgtttttct tctttgcgct
     5101 gattaagctc aaactgaaag gatacgtcgc cgcctcatgg acggtagcaa tcgcccttgc
     5161 cgtggctttg ctgttctata aaatgccggt cgctaacgcg ctggcctcgg tggtttatgg
     5221 cttcttctat ggtttgtggc ccatcgcgtg gatcattatt gcagcagtgt tcgtctataa
     5281 gatctcggtg aaaaccgggc agtttgacat cattcgctcg tctattcttt cgataacccc
     5341 tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc ggcgctttcc ttgaaggagc
     5401 cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg ctggtcggcc tgggttttaa
     5461 accgctgtac gccgccggac tgtgcctgat tgttaacacc gcgccagtgg catttggcgc
     5521 gatgggcatt ccaatcctgg ttgccggaca agtaacaggc atcgacagct ttgagattgg
     5581 ccagatggtg gggcgtcagc taccgtttat gaccattatc gtgctgttct ggatcatggc
     5641 gattatggac ggctggcgcg gtatcaaaga gacgtggcct gcggtcgtgg ttgcaggcgg
     5701 ctcgtttgcc atcgctcagt accttagctc taacttcatt gggccggagc tgccggacat
     5761 tatctcttcg ctggtatcac tgctctgcct gacgctgttc ctcaaacgct ggcaaccagt
     5821 tcgcgtattc cgcttcggtg acttgggggc gtcacaggtt gatatgacgc tggcccacac
     5881 cggttacacc gcgggtcagg tgttacgtgc ctggacaccg ttcctgttcc tgaccgctac
     5941 cgtaacactg tggagcatcc cgccgtttaa agccctgttc gcatcgggtg gtgcgctgta
     6001 tgagtgggtg atcaacattc cggtgccgta cctcaataaa ctggttgccc gtatgccacc
     6061 agtggtcagc gaggctacag cctatgccgc cgtgtttaag tttgactggt tctctgccac
     6121 cggcaccgcc attctgtttg ctgcactgct ctcgattgtc tggctgaaga tgaaaccgtc
     6181 tgacgctatc agcaccttcg gcagcacgct gaaagaactg gctctaccca tctactccat
     6241 cggcatggtg ctggcgttcg cctttatctc gaactattcc ggactgtcat caacactggc
     6301 actggcactg gcgcacaccg gtcatgcatt caccttcttc tcgccgttcc tcggctggct
     6361 gggggtattc ctgaccgggt cggatacctc atctaacgcc ctgttcgccg cgctgcaagc
     6421 caccgcagca caacaaattg gcgtctctga tctgttgctg gttgccgcca ataccaccgg
     6481 tggcgtcacc ggtaagatga tctccccgca atctatcgct atcgcctgtg cggcggtagg
     6541 cctggtgggc aaagagtctg atttgttccg ctttactgtc aaacacagcc tgatcttcac
     6601 ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc ttaacgtgga tgattcctta
     6661 atgattgttt tacccagacg cctgtcagac gaggttgccg atcgtgtgcg ggcgctgatt
     6721 gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg agcgccaact ggcgatgcaa
     6781 ctcggcgtat cacgtaattc actacgcgag gcgctggcaa aactggtgag cgaaggcgtg
     6841 ctgctcagtc gacgcggcgg cgggacgttt attcgctggc gtcatgacac atggtcggag
     6901 caaaacatcg tccagccgct aaaaacgctg atggccgatg atccggatta cagtttcgat
     6961 attctggaag cccgctacgc cattgaagcc agcaccgcat ggcatgcggc aatgcgcgcc
     7021 acacctggcg acaaagaaaa gattcagctt tgctttgaag caacgctaag tgaagacccg
     7081 gatatcgcct cacaagcgga cgttcgtttt catctggcga ttgccgaagc ctcacataac
     7141 atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc tgcaatcctc agtgaagcat
     7201 agccgtcagc ggatgtatct ggtgccaccg gttttttcac aactgaccga acaacatcag
     7261 gctgtcattg acgccatttt tgctggtgat gccgacgggg cgcgtaaagc aatgatggcg
     7321 caccttagtt ttgttcacac caccatgaaa cgattcgatg aagatcaggc tcgccacgca
     7381 cggattaccc gcctgcccgg tgagcataat gagcattcga gggagaaaaa cgcatgatta
     7441 tttccgcagc cagcgattat cgcgccgcag cgcaacgcat tctgccgccg ttcctgttcc
     7501 actatatgga tggtggtgca tattctgaat acacgctgcg ccgcaacgtg gaagatttgt
     7561 cagaagtggc actgcgccag cgtattctga aaaacatgtc cgacttaagc ctggaaacga
     7621 cgctgtttaa tgagaaattg tcgatgccgg tagcactggc accggtgggt ttgtgtggca
     7681 tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc ggacgcgcat ggcattccgt
     7741 ttactctctc gacggtttcc gtttgcccga ttgaagaagt cgcgccagcc atcaagcgcc
     7801 caatgtggtt ccagctttat gtactgcgcg atcgcggctt tatgcgtaac gcgctggagc
     7861 gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt ggatatgccg acaccgggcg
     7921 cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa tgcggcaatg cgccgctact
     7981 tgcaggcggt gacgcatccg caatgggcgt gggatgtggg cctgaacggt cgtccacatg
     8041 atttaggtaa tatctcagct tatctcggca aaccgaccgg actggaagat tacatcggct
     8101 ggctggggaa taacttcgat ccgtccatct catggaaaga ccttgagtgg atccgcgatt
     8161 tctgggatgg cccgatggtg atcaaaggga tcctcgatcc ggaagatgcg cgcgatgcag
     8221 tacgttttgg tgctgatgga attgtggttt ctaaccacgg tggccgccag ctggacggtg
     8281 tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc ggtgaaaggt gatatcgcca
     8341 ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt gcgtatgatt gcgctcggtg
     8401 ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct ggcaacagcg ggccaggcgg
     8461 gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa agtggcgatg acgctgactg
     8521 gcgcgaaatc gatcagtgaa attacgcaag attcgctggt gcaggggctg ggtaaagagt
     8581 tgcctacggc actggctccg atggcgaaag ggaatgcggc atagtcgttt gcgccccctc
     8641 accctaaccc tctccctcag ggagagggga ccgttcggcg ctgtatgtac tccctcactc
     8701 tgaaacgaca ccgcactctt tttttctccc tcgcccctcc ggggagggcc ggggtgaggg
     8761 gaaaaggccg cacggttcac caattttctg ctatcctgcc cccgcactaa gggggcagta
     8821 tgctaaacat cgtactttac gaaccagaaa ttccgccaaa tactggcaac atcatccgtc
     8881 tttgcgctaa taccggcttt cgtctacata tcatcgaacc gatgggattt gcctgggacg
     8941 acaagcgcct gcgccgcgcg gggctggact atcacgagtt taccgccgtt acgcgtcatc
     9001 atgactatcg cgcgttcctc gaagcagaaa atccccagcg cctgtttgcc ctcaccacga
     9061 aaggtacgcc tgctcacagc gccgtaagct atcaggatgg cgattatctg atgttcggcc
     9121 cggaaacacg cggcctgcca gcgagcattc ttgatgccct gcccgctgaa caaaaaattc
     9181 gcattccgat ggtgccggac agccgcagca tgaatctgtc caatgcggtg tcggtagtgg
     9241 tgtatgaagc ctggcggcag ctgggatatc cgggagcgtt gttgagagat tagttactgt
     9301 atgccggata gatgattaca tcgcatccgg cacgatcaca ggacattaga tcccatcccc
     9361 atactcaaat gtatggttaa taccgttgaa atgctggtcc atatccattg atggcttatc
     9421 gctgtctggt ttaccgacaa tacgagccgg aacgccagcg gcggttgtat gcggcggcac
     9481 cggttgcagc accacggaac ctgcgccaat cttcgcgccg caaccaactt caatattgcc
     9541 gaggattttc gcgcccgcgc caatcatcac accttcacga attttcgggt gacggtcacc
     9601 accagattta cccgtaccgc caagcgtcac agattgcaga atcgatacgt cgttttcaat
     9661 taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg agcataatgc cacggccaat
     9721 ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa acctggtttt gcagaaagat
     9781 tgccaatgcg cgacgcccct gattccacaa ccagtgaccg atgcgatagg cctgcaaggc
     9841 atgaaaaccc ttcaggtata acaacggggt tgagtattta tcgactgccg ggtcgcgggt
     9901 acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt tccgggtcag cggcgtaggc
     9961 ttcttcaacc acttcacgga tagcaatagc aggcataatt ggcgatgaca gcttgttcgc
    10021 cagcatgtag ctcagtgcac tgccaaggtt ttcgtgcttg agtagcgtcg cgtggtaaaa
    10081 actggccagc attggctcac agtccgccag cgttctggct tcggctttaa tattgttcca
    10141 gacaatttcc agttcttcac acgacattgc ttactccaca cgatgagata atgaccggcc
    10201 cgttctgcgc gggccgggtc atagcggtaa caaaggttcc ctggggttag tgactgctgc
    10261 gctcgtcctt gcgtgcacga cctaataatg tcaatgctgc ctcgcgcgcg ttttttccgc
    10321 aatataatac ttgataaatt tcctcggtta ttggcatttc aacgccgaag cgatgcgcca
    10381 gttcgcggac ttctttcgta ttgcggtagc cttccaccac ctgaccaatc ttctcctgcg
    10441 cgctttgtac atccatgccc tgaccgagca tcataccaaa acggcggtta cgcgactggt
    10501 tgtcggtaca ggtaagcacc agatcgccca gccccgccat gcccataaag gtggcaggat
    10561 cggcacccag cgccgcacca agacgcgaca tttcagccag cccacgggtg atcagcgccg
    10621 tacgcgcatt cgcaccaaaa ccgataccgt cagacatccc cgcgccaatg gcaatgacgt
    10681 ttttcaccgc gccgccaagc tgcacgccaa tgaaatccgg gttgctgtaa acgcggaaac
    10741 ttttgccgca gtgcagcagc tgctggagat catcggcaaa ggtctgatcg gtcgaggcca
    10801 gcgaaatagc tgtcggtaaa cctgccgcca gttctttcgc aaacgttggg ccagagataa
    10861 ccgccagcgg aatttgatcg cctaaggcct cacgcgccac gtcctgtaac agacgtccgg
    10921 tttccgcttc cagccctttg gtcgcccaca ccagacgcgc atcaggacgc atcagcggtt
    10981 taatctggcg cagcacttca ccaaagacat ggctgggtac gacgacgaga atattacgac
    11041 tggctgccag cgcagtggcg agatcgcttt caagatggag cgtatcggga aaaggcacat
    11101 cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt tgcgatatgt tcagggtcat
    11161 ggccccagag gacaacctcg tggccatttc ttgccagggt aatggcaaga gcggtgccgt
    11221 acgagccggc accgatcaca gtcattgaag cattacgttg gttcatcagg catcctgatg
    11281 ttcttcagta ccttcgccag cctgctgctg caaatagttc atgaacagcg catcgaagtt
    11341 aaccggcgca aggttcagtt gcgggaatgt accgcgggat accatgctgg tgatgcactc
    11401 acgagcatac gggaacagaa tgttcgggca gtatgcgccc aggcaatgtg ccatctgggt
    11461 gccttcgata cccgcgatgg agaaaatacc gccctgctga acttcacaca ggaacgcggt
    11521 ttcttcgccc aaggaggccg ttacggtaac acgcagtacc acttcgtata cgtcatctgc
    11581 cagttgggaa gatgccgtat ccagatcaag tttaacttct ggttgccaat ctttctggaa
    11641 aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg gtataaatac gttggatctg
    11701 gaaagtcatt tcagtgttgt tttgttctga catgtgtaga aaacccttaa gtgttgtcct
    11761 taaatactgc gcaatgtaat accaacgttc acacgttatt tcagcagggg atccagtcca
    11821 ccacgtgcat ccaatgcata caagtcatca cagccgccaa tgtgctgtgc gtcaataaaa
    11881 atctggggaa ccgtggtgca accgctgcgt ttgatcatct cttcacgctt ggcggcattg
    11941 ccatcgatcg gcaactcctg gaaactcacg cccttgctgc tcagcagtgc ttttgcacga
    12001 tggcaatacg ggcaggtttc tttggtatag atttcaacat tggccataat ttagctcctg
    12061 tttttttacc ctgaaatttc atgttgcagg gcggctgcaa aatggagtgt ccccagaagc
    12121 ttataacaat aagtcgctgg gggcctaatt gcagctaacg gcctgcatca tgaaagacga
    12181 caggtaaatt atttgccgcg caccagaggc aagttttcgc cagcccagcc agcaacgccc
    12241 tctttcagca cgaatacttg cgcaaaacca gctttcgtca gcgcgtttgc aggctcctgg
    12301 cactgcatgc cagaaccgtc taccacgata accgatttgt ctttgtgctt ctcaagctca
    12361 ccaacattgt tggctttgat ttcgctcggc aacagattga tagaacctgc gatatggcct
    12421 ttacggaagt catcacgctg acgtaaatcc acaaccacag cgtcttcttt gttgatcaga
    12481 cgcgtagctt caccacgagt aatcaccttc actttcgagg tcaggctctt aaacgtagtc
    12541 acaagaaccg ccaccagtaa cgcgatccag gcgatactca gtatgggatg acggccaaca
    12601 aattgcataa tttcttgcat ggggggtaac aactcccgac gtagtgatta aaaaaaccag
    12661 gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc gcgttaactg gaatgcaatt
    12721 ttgcggggca cgacgaaaaa aaagcgcaaa tctggcatcg gcttgccgcg agcggtcgta
    12781 tattttgatc ttcagaggct attttatcga ttcagctgta gtaaaattac gcaaattttg
    12841 actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa aacctatggt actggtgatt
    12901 ctggatggct atggctatcg tgaagaacag caggataacg ccatttttag tgctaaaacc
    12961 ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc tgatcgacgc ttccggtctg
    13021 gaagtcggtc tgcctgaccg tcagatgggt aactccgaag taggccacgt taacctgggt
    13081 gccggccgca tcgtgtatca ggacctgact cgtctggacg ttgaaatcaa agatcgcgct
    13141 ttctttgcta atccagtgct ggctggcgcg gtacataaag cgaaaaacgc aggtaaagca
    13201 gtacacatta tgggtctgct ctccgcaggc ggcgtacaca gccacgaaga tcacatcatg
    13261 gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa tctacctgca cgcattcctt
    13321 gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc tgaaaaaatt tgaagagaaa
    13381 tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg gtcgttacta cgcgatggac
    13441 cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc tgctgactct ggcgcagggt
    13501 gagttccagg ccgatactgc cgttgctggt ttgcaggcag cttatgctcg cgacgaaaac
    13561 gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc agccagatgc ggcaatggaa
    13621 gacggcgatg cgctgatttt catgaacttc cgtgctgacc gcgcgcgtga aatcactcgt
    13681 gcattcgtga acgctgattt cgatggcttc gcgcgtaaga aagtggttaa cgtcgatttc
    13741 gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg ttgcttaccc acccgcttcc
    13801 ctggttaaca ccttcggcga gtggatggcg aaaaacgaca aaactcagtt gcgtatttcc
    13861 gaaaccgaaa aatatgccca cgttactttc ttcttcaacg gtggcgtaga agagtcgttc
    13921 aaaggcgaag atcgcattct gatcaactca ccgaaagtgg ctacctacga tctgcaaccg
    13981 gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg ccatcaagag cggcaaatac
    14041 gacaccatta tctgtaacta tccgaacggc gacatggtag gtcacaccgg ggtgatggaa
    14101 gcggcggtta aagcggttga agcactggat cactgcgtgg aagaagtcgc gaaagcggtt
    14161 gagtccgtgg gtggacaact gctgatcacc gctgaccacg gtaacgctga acagatgcgc
    14221 gatccggcaa cgggtcaggc acacacggca cacaccaacc tgccagttcc gctgatttac
    14281 gttggtgata agaacgtgaa agcggttgca ggcggcaaac tttctgacat cgcgccgacc
    14341 atgttgtcgc tgatgggtat ggaaatcccg caagagatga ctggtaagcc gctgttcatc
    14401 gtggaataat ccctccccat gaggggaaag gcgattaata ccatgacacg ggccgtgaaa
    14461 ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg ttcttagcgc tggcgtattg
    14521 ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac tcaaatctat tcaggccgat
    14581 atcgccgcaa aagagcgcgc ggtacgccaa aagcaacaac aacgcgcaag cctgctcgca
    14641 caattgaaaa agcaggaaga agcgatctct gaagccaccc gtaagctgcg cgaaacgcaa
    14701 aacacgctca atcaactcaa taaacagatt gatgagatga acgcgtcgat tgccaaactg
    14761 gagcagcaaa aagccgccca ggagcgcagc ctcgccgcac aactggatgc cgcattccgt
    14821 cagggcgagc ataccggtat tcagctaatt ctcagcggtg aagaaagcca gcgtggacag
    14881 cgtttacagg cttatttcgg ctatctcaac caggcgcgac aagaaaccat tgcccagttg
    14941 aagcaaacgc gtgaagaagt caccatgcag catgctgaac tggaagagaa acaaagcgag
    15001 caacaaacgc tgttatatga acagcgcgcc caacaggcga aactgactca ggctctgaac
    15061 gagcgtaaaa agacgctggc ggggctggaa tcttccatcc agcaaggtca gcagcagcta
    15121 agtgaactgc gtgccaacga atcccgtctg cgtaacagca ttgcccgtgc tgaagccgcg
    15181 gcgaaagcgc gtgctgaacg agaagcgcgt gaggcccagg cggttcgcga gcgccagaaa
    15241 gaagcgacgc gcaaaggcac cacctacaaa ccgaccgaaa gcgaaaaatc gctgatgtcc
    15301 cgaactggtg gcctgggtgc gccgcgtggt caggcattct ggccggttcg cgggccgacg
    15361 ctgcatcgct atggcgagca gctacagggt gaattacgct ggaaagggat ggttatcggt
    15421 gcctctgaag gtactgaagt taaagcaatt gccgacggtc gagtgattct ggctgactgg
    15481 ctgcaaggtt acggtctggt ggtggtggtt gagcacggta aaggcgacat gagtctttac
    15541 ggctataatc agagcgcact ggtgagcgtt ggttcgcagg ttcgcgcggg ccagccaatt
    15601 gcactggtgg gcagcagtgg cggtcagggt cggccttcac tctatttcga aattcgccgc
    15661 cagggtcagg cggtcaatcc acagccgtgg ttgggaagat aagttttgtt tccatttcgt
    15721 cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct cccccgtact tgctggcaaa
    15781 cttgccatcg tcattgatga ttttgggtat cgcccgcaca acgaaaacca ggtgctggcg
    15841 atgccttccg ctatctccgt cgctgtatta cccgattcac cgcacgccag agagatggcg
    15901 accaaagcgc ataacagcgg tcacgaagtg ttgattcatc tcccgatggc accgttgagt
    15961 aaacagccgc tggagaaaaa tacgctacgc ccggagatga gcagcgacga aattgagcgc
    16021 attattcgta gtgcggtcaa taacgtgccc tatgccgtgg ggatcaacaa ccacatgggc
    16081 agcaagatga cctctaacct gtttggtatg cagaaagtga tgcaggcgct ggagcgttac
    16141 aatctttact tcctcgacag cgtaaccatc ggtaataccc aggcgatgcg cgccgcgcaa
    16201 ggtactggcg tgaaggtgat caaacggaag gtgttcctcg acgattcgca gaacgaagcg
    16261 gacatccgtg tgcaatttaa tcgcgcaatt gatctggcgc gtcgcaacgg ttcaaccatt
    16321 gccattgggc atcctcaccc ttcaacggta cgcgtgctac aacagatggt ttataacctg
    16381 ccgccagaca ttacgctggt gaaagccagc agcttgctga atgaaccgca ggttgatact
    16441 tctacaccgc cgaaaaacgc tgtgcctgat gcaccgcgta atccgttccg tggcgtgaag
    16501 ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta atcgcttctt tgaagtatta
    16561 agcgaaagca tcagccagag cacgctgatc gtttacttcc agcatcagtg gcaaggctgg
    16621 ggcaaacagc ccgaagcggc gaagtttaac gctagcgcaa attaaaggcg ctacgcgtaa
    16681 tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa gcggaaagac cacagtaacg
    16741 cctggtaacc gaccttcacg ctgcgcacat tggtaatcag gcgcttatac atacccggaa
    16801 gtaaatatct cggcaatcat ccgttggcgg gtaagaatat ccggctcttt gcg
//
LOCUS       DAZPEU010000075        16430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPEU010000075 DAZPEU010000000
VERSION     DAZPEU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.075"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1025)
                     /locus_tag="ACHU2L_002211"
     CDS             complement(<1..1025)
                     /locus_tag="ACHU2L_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1357055.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACHU2L_002212"
     CDS             complement(1127..2107)
                     /locus_tag="ACHU2L_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR1357056.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACHU2L_002213"
     CDS             complement(2115..2825)
                     /locus_tag="ACHU2L_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1357057.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHU2L_002214"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHU2L_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR1357058.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACHU2L_002215"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACHU2L_002215"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR1357059.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACHU2L_002216"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACHU2L_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR1357060.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACHU2L_002217"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACHU2L_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR1357061.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACHU2L_002218"
     CDS             9402..9512
                     /locus_tag="ACHU2L_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR1357062.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHU2L_002219"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHU2L_002219"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR1357063.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHU2L_002220"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHU2L_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR1357064.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACHU2L_002221"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACHU2L_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACHU2L_002222"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACHU2L_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR1357065.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHU2L_002223"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHU2L_002223"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR1357066.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         3976 a         4235 c         4022 g         4197 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta
//
LOCUS       DAZPEU010000076        16416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPEU010000076 DAZPEU010000000
VERSION     DAZPEU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.076"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHU2L_002224"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHU2L_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHU2L_002225"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHU2L_002225"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR1357067.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHU2L_002226"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHU2L_002226"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR1357068.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHU2L_002227"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHU2L_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHU2L_002228"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHU2L_002228"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR1357069.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHU2L_002229"
     CDS             complement(5877..6218)
                     /locus_tag="ACHU2L_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR1357070.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHU2L_002230"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHU2L_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR1357071.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHU2L_002231"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHU2L_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR1357072.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACHU2L_002232"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACHU2L_002232"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR1357073.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACHU2L_002233"
     CDS             12897..14240
                     /locus_tag="ACHU2L_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR1357074.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACHU2L_002234"
     CDS             complement(14302..14508)
                     /locus_tag="ACHU2L_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR1357075.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACHU2L_002235"
     CDS             14833..15387
                     /locus_tag="ACHU2L_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1357076.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="ACHU2L_002236"
     CDS             complement(15450..16388)
                     /locus_tag="ACHU2L_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357077.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
BASE COUNT         3929 a         4459 c         3995 g         4033 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattt
//
LOCUS       DAZPEU010000077        16319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPEU010000077 DAZPEU010000000
VERSION     DAZPEU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.077"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            65..704
                     /locus_tag="ACHU2L_002237"
                     /pseudo
     CDS             65..704
                     /locus_tag="ACHU2L_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            807..1130
                     /locus_tag="ACHU2L_002238"
     CDS             807..1130
                     /locus_tag="ACHU2L_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR1357078.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1197..2654)
                     /gene="nuoN"
                     /locus_tag="ACHU2L_002239"
     CDS             complement(1197..2654)
                     /gene="nuoN"
                     /locus_tag="ACHU2L_002239"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR1357079.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2661..4190)
                     /gene="nuoM"
                     /locus_tag="ACHU2L_002240"
     CDS             complement(2661..4190)
                     /gene="nuoM"
                     /locus_tag="ACHU2L_002240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR1357080.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4354..6195)
                     /gene="nuoL"
                     /locus_tag="ACHU2L_002241"
     CDS             complement(4354..6195)
                     /gene="nuoL"
                     /locus_tag="ACHU2L_002241"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR1357081.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6192..6494)
                     /gene="nuoK"
                     /locus_tag="ACHU2L_002242"
     CDS             complement(6192..6494)
                     /gene="nuoK"
                     /locus_tag="ACHU2L_002242"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR1357082.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6491..7045)
                     /gene="nuoJ"
                     /locus_tag="ACHU2L_002243"
     CDS             complement(6491..7045)
                     /gene="nuoJ"
                     /locus_tag="ACHU2L_002243"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR1357083.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7057..7599)
                     /gene="nuoI"
                     /locus_tag="ACHU2L_002244"
     CDS             complement(7057..7599)
                     /gene="nuoI"
                     /locus_tag="ACHU2L_002244"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR1357084.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7614..8591)
                     /gene="nuoH"
                     /locus_tag="ACHU2L_002245"
     CDS             complement(7614..8591)
                     /gene="nuoH"
                     /locus_tag="ACHU2L_002245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR1357085.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8588..11314)
                     /gene="nuoG"
                     /locus_tag="ACHU2L_002246"
     CDS             complement(8588..11314)
                     /gene="nuoG"
                     /locus_tag="ACHU2L_002246"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR1357086.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11367..12704)
                     /gene="nuoF"
                     /locus_tag="ACHU2L_002247"
     CDS             complement(11367..12704)
                     /gene="nuoF"
                     /locus_tag="ACHU2L_002247"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR1357087.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12701..13201)
                     /gene="nuoE"
                     /locus_tag="ACHU2L_002248"
     CDS             complement(12701..13201)
                     /gene="nuoE"
                     /locus_tag="ACHU2L_002248"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR1357088.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13204..15006)
                     /gene="nuoC"
                     /locus_tag="ACHU2L_002249"
     CDS             complement(13204..15006)
                     /gene="nuoC"
                     /locus_tag="ACHU2L_002249"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR1357089.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15100..15762)
                     /gene="nuoB"
                     /locus_tag="ACHU2L_002250"
     CDS             complement(15100..15762)
                     /gene="nuoB"
                     /locus_tag="ACHU2L_002250"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR1357090.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15778..16221)
                     /gene="nuoA"
                     /locus_tag="ACHU2L_002251"
     CDS             complement(15778..16221)
                     /gene="nuoA"
                     /locus_tag="ACHU2L_002251"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR1357091.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         4040 a         4618 c         4270 g         3391 t
ORIGIN      
        1 aaataaacgc tcataattca ttataaataa taatgaaatg acgcctttta ttgataattt
       61 acatatgaaa aaattttcct tttgttatat tatttctgtt ttcactccct ctgattattt
      121 ttaccaaccc tgggtcactg cattatcgcc aacgccccga cacgtaagtc ctgagcaatt
      181 agaaaaaaca gttcgttatc ttacacaaac tgtgcatcca cgtaatgccg gcaatattga
      241 taatctgaat aggtccgcag agtatataaa agagttcttt gtcagtagcg gtgccagagt
      301 taccgcgcag ggcgtccccg attggctatt acgactctgc gcctggaagc cagaattacc
      361 cttacccggc aatgtcctgg ctttatcccg atcggggaga ctttattgct gtggtcggca
      421 gaatgcagga tatcaacgcc gttcgtcagg taaaagcggc attattgtca tcccgggatt
      481 tatctgttta ttctatgaat gccccagggt ttattcccgg tattgatttt tctgatcacc
      541 tcaattattg gcaacacgat attcccgccg taatgattac tgacaccgct ttttatcgta
      601 ataaacaata ccacttgccc ggtgataccg cagacagatt gaattatcag aaaatggctc
      661 aggtggtaga tggtgttata accttgttat acaacagtaa ataataaaaa tttctcgagg
      721 cgggtccaga aacagcgaca tgtacatttt attttccctc gtctgtttta agatgcgaaa
      781 cataaaattc aatggaggaa aaataaatgg gaatgattgg ctattttgca gaaattgact
      841 cagaaaagat taaccaactg cttgagtcta ctgaaaaacc tttgatggat aatatacatg
      901 acacgctttc aggattacgc cgactggaat cactggatcg aagtgagctg cgtaaacagt
      961 tttccattaa gcgactcaat gaaatggaga tttatccggg agtgaccttt tcagaagagt
     1021 tagaggggca actctttgcc tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc
     1081 gacgtatgct gcgacaaggg aatcatgcgc tgacggttat tgttgggtga tggtgtcaga
     1141 gcgccaaaaa aaaccgccga acatgtcggc ggctttctga cttacaaact aacagtttac
     1201 atcagcggca ttgctaaccg cacaatacta atcagcggtt gtggccatac acccagcacc
     1261 agtaccaaca gtgcagaaat cagcaccacg ataccgcccg cgctgtactg ccagtttgac
     1321 ggtgcatcgc gacccggttg ttccggtgcg tgcaggtaca ggctcaccgc cacgcgcagg
     1381 tagtagtaga ggccgattgc cgaaccgaca accacggcac ccaccagcca ccacaagtgt
     1441 gcctggacac cgaccgccag cacgtagaac ttaccgataa agcccagcgt catcgggata
     1501 ccggccagag acagcatcat caccgtcatc actgccgcga ggatcggacg atgccagaac
     1561 agaccgcggt aagagaacag ggaatcagca tccgggccac gatacgggct ggacatcagg
     1621 ctgaccacgc cgaacgcgcc gaggctgctg aacagataac cggccaggta aacccctacc
     1681 gcttccatcg acatctcgcc ggtttgcagc gcaatcagcg ccaccagcag atagccgagg
     1741 tgagagatag atgagtaacc gagcagacgt ttgatgttgg tctggctcaa cgccatcagg
     1801 ttaccgaaga tgatggaggc aaaggcgata atcgccagta ctacgcgaat cgcttcgctg
     1861 tcacccaccg gtgcgtagag gaacagacgc atcaccacac cgaagatagc gattttgctc
     1921 gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac
     1981 aggtggaacg gcaccagaga gagtttgaag ccgaggccaa caatcatcat gccgaaacct
     2041 gccagcaaca gcggctcgtt gagcataccg tcgccgaggt ttttacccag cgcgacaaac
     2101 gacaggtcgc cagactgcgc atacaccagc gccataccga acagcaggaa agaagacgct
     2161 gcggcagaaa ggatggtgta tttgatactg gcttccagcg aacgtttctg gcggaaagcg
     2221 taaccgacca ggccaaacag cggcaaagag atcagttcaa taccgaggaa cagagacgcc
     2281 agatggtttg cattcgccag caggatcccg cccagcgcgg caattaacac caacaggtag
     2341 aactcatcct tgttgtcgtt atagccttca agccacgggt aggcgaaagt acaggtggcg
     2401 aggctcgcca acaataccag cccggtgtaa agcatggcga aaccatcaac gcgcatcagc
     2461 ggcgtaacgt ccatagcgcc cgcctggcca acaaaccaga gcgaaaccag cgccgcgtta
     2521 agcccaataa ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc aatggagagc
     2581 atcacaacca ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat caggttttgt
     2641 ggagttattg tcatggcgat ttacggcctt gtagtagtaa cggaattaac aaaccactgc
     2701 tggatattgc caatcgcgga gtgcgaggta tccagaatcg gctgcggata gaagcccagc
     2761 agtaccagca gcaccaccag caacaggatc ataaacagct cacgcagcga catccctggc
     2821 agttcctggc tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg caacatcgcc
     2881 agcgaataaa cagatgcaaa gaccagccca aaggtagaga taacggtaat caccgggaca
     2941 acctggaagc tgccgaacag aatcataaat tcgccgacga agttaccggt gccaggcatc
     3001 ccaagcgttg ccaccgcaaa gaacagcgac agtgctggca gccatttcat cttgctccac
     3061 agaccgccca tcatgcgcat gtcgcgggta tggatacgtt cataaagctg accgcagaga
     3121 ataaacagac ccgccgccga caagccgtgc gcaatcatct ggattaccgc gccctggtag
     3181 gccaactggc tgccggtgta gattgcaatc agcacgaagc ccatgtggga aaccgaggtg
     3241 taggcgatca gacgtttgat atcggtctga gcgaaggtca tccacgcacc gtagaagatg
     3301 ccgataacac ccagccacat agcgattggc gcgaactctg ccgacgcgtt cgggaacagc
     3361 ggcagggaga aacgcaacaa accgtaagcg gcggttttca gcaagatccc cgcgaggtcc
     3421 acggaaccgg cggtcggagc ctgggagtgc gcatccggca gccagccatg cagcggaacc
     3481 accggcattt tgactgcgaa ggcgatgaag aagcccagca tcaacagata ttccacacca
     3541 ctggacattg gcgtattcag cagctcttca tagttgaagg tccagacgcc ggtcgcattg
     3601 tagtgaacaa aaaccagcgc caggatggcg atcaacatca ccagaccact cgcctgggtg
     3661 taaatgaaga acttggttgc cgccgtgata cgcgttttac cgtcagaggc tttatgcccc
     3721 cacagtgcga tcaggaagta catcggcacc agcatcattt cccagaagaa gaagaacagg
     3781 aacatgtcga tggcaaggaa cacgccgata acgccgccca ggatccacat caggttgagg
     3841 tggaagaagc cctgatattt ttcgatctct ttccacgaac atagtaccgc cagcacaccg
     3901 agcagaccgg tcagcacgac catcagcagc gacagaccgt caatggcgag atgaatagag
     3961 ataccaaaac gcgggatcca cggcatgtcg aattcagact gccactgcgg aattccggcg
     4021 gattgcgtca gtgaataacc gccctgcaac cacagttgca gcgacagcgc cagcgtcaat
     4081 cccatggtga tcagcgcgat ccagcgtggc accttgacgc caaagcgttc ggtctgccag
     4141 cacaggaagc cgccaataaa gggaattaat attagccagg gtagtaacat ggcgatcttt
     4201 attccttgta aaagtcccgt caggaccgga ttttcaacga attctcacga cagaaaattc
     4261 tcttcggact gggggtagtg tcggatgcgc cgcgaaggca gcttatccga cctacgttct
     4321 tatatgccca ggggcaatcc cacaatcctt aactcaacgc agtaccatca acagtgccag
     4381 caccacgacc gcaccgatgc tcatggatgc cacataccag cgcagataac cgttctcgct
     4441 taacagcaga cctttacctg caaagcggga aagaaccgcc gggatgttca tcattgagtt
     4501 cagcggatcg cgtttcagca accaggcaat acccaggaac ggcttgacga acactttgtc
     4561 gtacagccag tcaaagcccc aagcgttgta ccaccaggta ctcagcaggc ggcccggcgc
     4621 actgttggcg atggaggtca ccagagaacg tttacccagc cacagccagg ctgccagcag
     4681 aatgccgacc accgcgacca cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc
     4741 cagttccgtc gtttgcggaa gcacgccctg cagcggcggt acaatcagtg cgccaacgaa
     4801 ggtggaaagg atcagcagca caatcagcgg caggctgtga gttacccctt tcacggcgtg
     4861 agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc atacggaagg tgtagagcga
     4921 ggtcataaac gcaccgacca gacctgccac catcagattg atatgaccat tcgccatcgc
     4981 acccgcgagg atctcatcct tactgaagaa gcccgcagtg accagcggca gtgccgacag
     5041 tgctgcgccg cccaccagga agcagagata aaccagcgga atagatttac gcagaccgcc
     5101 catcttgaag atgttctgtt cgtgatggca ggccagaatg acggaaccgg atgccaggaa
     5161 cagcagcgct ttaaagaacg cgtgagtcat caagtggaaa atcgccgcat cccatgcctg
     5221 cacgccaagc gcgaggaaca tgtagccaat ctggctcatg gtagagtaag cgagaacacg
     5281 tttgatgtcg gtctgtacca gcgcggcaaa accggccagc agcagcgtaa ccgccccgac
     5341 aatacccacc agatgcagaa cttccggcgt catcaggaac aggccgtggg tacgggcgat
     5401 caggtagaca cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg
     5461 gcccgccatc gcgtcagcaa gccatgtctg caacggcaac tgcgcagatt taccgaccgc
     5521 accgcccagc agcatcagcg tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc
     5581 tggtgccagt tccaccatat cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa
     5641 cagtgcgaaa gcgaggaaaa cgtcacccac acgggtcacg acgaacgctt tcattgctgc
     5701 tgcgccattc ttcggatcgg tgtaatagaa accgatcagc agataggagc acaggcccac
     5761 gccttcccag ccgaggtaca tcagcagcag gttatcggcg agaaccagaa ctaccatgct
     5821 ggcgatgaac aggttggtgt aggcgaagaa gcgagagtag ccctcttcac cgcgcatgta
     5881 ccaggaggcg tacatgtgaa taaggaaacc cacaccagtg accaccgaga gcatggtcag
     5941 cgacaggccg tccagcacca ggttaaaacc gatgttaaag tcgcctaccg acatccacgt
     6001 ccacagcggc tggctgtatg cctgctcgcc gttagcgaag aaatcaacgc cgataaaggc
     6061 ggttaccagc gccgccaggc ccacagagcc aacgccgacg atcgccgaaa cgttttcaga
     6121 ccagcgccca cgggagaacg ccagcaggac gaagccaatc aatggcaaaa taatggttaa
     6181 ggcaagcatg ttcatccgcg catctcactt actgaatcga tgttcaggtt ctggcgacga
     6241 cggtgaagtt gcagcagcag cgcaaggccg atactcgctt ctgccgccgc gaggctgatg
     6301 gcgagaatgt acatcacctg accgtcggtc tgaccccagt agcttccggc gaccacaaag
     6361 gccagcgcgg aggcgttaat catgatttcc agaccaatca gcataaacag cagattgcga
     6421 cggataacca gaccggttaa gccaagaacg aataagattg ccgcgaggat cagtccatgt
     6481 tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc
     6541 tcagcacttc acccgcacgc tcttcacgac cgacgtggaa ggccacaacc agacccgcga
     6601 gcagcagcat agaagccagt tccaccgcca gtacgtaagg cccgaacagc gtaataccca
     6661 ctgctttagc actgattggc gtaccgtcga taccctgatc gttaacaccg aggatggcgt
     6721 aaacaatcac caccagcatg atggccgaca aaattgccgg gccaatccac acctgcggtt
     6781 tcagccactg gcgttcctgt tcgatttctg aaccgcccag gttgagcatc atcaccacga
     6841 acacgaacag caccataatg gcacccgcgt agacgataat ttccagcgca ccggcgaagt
     6901 aagcgcctag tgagaagaac accccggaaa tcgccagcag cgaaataatc aggtacagca
     6961 gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc
     7021 cacagatata aaaagcgaac tccattgccc ctctccttac ggtaacaggc tcttgacgtc
     7081 gataggcttg gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc
     7141 catccggtag aagttatatt ccgggtattt gcccggaccg gagatcagca gatcctcttt
     7201 ctcgtaaacc agatcctggc gcttgtattc ccccatttcg aaatccgggg ttaactgaat
     7261 cgccgtggtc ggacaggctt cttcgcacag accacagaaa atgcagcgtg agaagttaat
     7321 gcggaaaaat tccggatacc agcgaccgtc tttggtttct gctttttgca gcgagataca
     7381 gccgaccggg caggctaccg cacagaggtt acaggctacg caacgctctt cgccgtccgg
     7441 gtcgcgggtc agaacgatac gaccacgata acggggcggc agatagaccg gctcttccgg
     7501 gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga tactacgaac
     7561 ctgggtgccg aaacctacta acaattcttt taaggtcatg gtcttattgc cccttattgc
     7621 gcctgccaga gaatgacagc cgccgttacc agcaagttga tcagcgtcag cggcaggcag
     7681 attttccagc cgaaggacat tacccggtca taacgcggac gcggtaacga cgcacgaatc
     7741 aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga accagatgaa tggcggtaac
     7801 aacgggcctt gccagccacc gaagaacagc gtcaccatca atgcagagat ggtcacaatc
     7861 ccgatgtact cacccacgaa gaacagaccg aacttcatac cggaatattc aatgtggtaa
     7921 ccatccgcca gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg acacaccgcc
     7981 acgcccgcga tggcaaaggt aataaaacca aagaattgcg ggatgacgtt ccacacatgc
     8041 gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc
     8101 agggaaagcc cgaggaacac ttcgtagctc agggtctgcg cagaagcacg catcgcaccc
     8161 agcagcgagt atttgttgtt acttgaccag cccgcaaaca gcaccgcgta aaccgccaga
     8221 cctgccatca tcaggaagaa caaaatcccg atgttcaggt cggcaaccac ccaacccgga
     8281 ctgactggca caattgcaaa ggccagcagc agcgaggtaa aggcaatcat cggtgccagg
     8341 gtaaagatga cgcgatccga gaatttcggg atccagtctt ctttaaagaa cattttgatc
     8401 atgtccgcaa ccagctggag cgaaccgccc cagccaacac ggttaggtcc ataacggttc
     8461 tggaacagac ccagcaggcg acgttcgcca aagctcatga atgccccaca ggtgacaacc
     8521 accagcagga tcaccaccgc tttgaggatg gtcagcagga tctcaatcag ttccggtgat
     8581 atccaactca ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc agcaccggag
     8641 caatgccgga catacccatc ggcaagccca cctgccctgc cgtcagtcct tcggcgattt
     8701 caaccggcag cgtgaccgtg ttgccatcgt aactaaagga gacgcgtgta cctgcgttca
     8761 cacccaactt cgcggcatcc gctgggttga gtttgatgta cggctgcggc atacggctct
     8821 ggaagaccgg agcacgctgt gacaattcat cgctgccaaa caggtggtag tacggcgcga
     8881 tacgccattt cccgtcctgc ggctggaagc gtgccggtac gctggtgaag taatccagac
     8941 cgttttcgct ggtttcaaac agacgcacgc ccggatcgcc aaagcgcagt ttgccgccca
     9001 cttcgtcctg gaatttgttc cacgcctgcg gggagttcca gcccggcgcc caggcaaacg
     9061 gcacttgcga acggtgcgcg gtcggctggt tgttaccttc catcgagaag gtgaacatgg
     9121 tgtcaatatc ctgcggctga cgcggctcat gaacgctgat attggcgcgc atggcggtac
     9181 gaccgctgta acggtgcggt tcacgggcca gtttctgccc acgaatacgg aatgtcgcat
     9241 ccggcgcagc atctttgata cccgccagtt ctgggatttt cgccacaaca gcgtcaatta
     9301 catggtcgag ctgcgtccag tccacttcac ggctcagcaa ggtgctgtgc agcgagtgca
     9361 gccagcgcca gctttccagc atgacagttt tgctgtcgta ataagccgga tcgtaaacct
     9421 ggaagaaacg ttgggcgcga ccttcattat taattaccgt accgtcgctt tcagcaaagc
     9481 tggcagagga aagcaccaga tgggcgtttt ccatgatcgc tgtgcgttga tgatcaacca
     9541 ccatcaccag cggcgcttta gccagcgcag cattcacgcg ggtagcagaa gcgtaacgat
     9601 gcagatcgtt ttccaacacc accaccgcgt cggcgcgtcc ggtttccagt tcggttaacg
     9661 cttcttcaag cgaaccgcca cccataatgc ccagccccat gctgttgacg gaacgggcaa
     9721 tcatggtgat accaacgtca gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag
     9781 cctgaatcac ctctatgcta ccggcgttcg tcccggagat aatcaacggt ttcttcgcac
     9841 ctgccagtgc ctgcacgatg acgtcgattt tgttttgcag ctcaggttcg ataccgtcaa
     9901 ccgctggtgc ggagttatcc agcgcatggg caatggcaaa acctaaacgc gcctgatctt
     9961 caaccggtgc gcggtaagtc cacgccgcga tatcatccag acgagtgtca tcaacgttgg
    10021 taacaaacag cggatgcttc gcacgttgac cgatgttgag gattgccgca atctgccagt
    10081 cagccacttt ctgtgctgca gccatttcgc gcgctttacc tttcaccgcc tgacgcactg
    10141 ccagtgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt
    10201 aagattcgat ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc actttcagcg
    10261 ccagttgcag acgttcctgc tcaccgtgag cgataccggt gtagaagttt tcttcgccca
    10321 ccagttcacg cagcgcaaag ttgctttcca cgctggcacg cggagaacca ataccgatca
    10381 ctttcttcga ctgacgcaga atatctgccg cgccctgcat tgcctgttcg gcgttgaggg
    10441 taatgaaatc atcgccacga cgctgtactg gctgacgcgg acgatccttc aggttgacgt
    10501 aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac
    10561 ggttttcgat acgacgcagt tcgccgtaac gttcaccggg gctgatgtta cagccgatgg
    10621 aacattgctg gcagatgctc ggcgcaaact gcatatccca tttacggttg taacgctcgg
    10681 agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt
    10741 cgctttccag cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc gcgccataaa
    10801 cgcccagatc tgtaccgtca gcgtaatctt tgtagtaacg cacacagcgg tagcaggcga
    10861 tgcagcggtt catttcgtga gagatgaatg gccccaaatc ctgattacgg tgggtacgtt
    10921 tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc
    10981 agttaccgcc ttcttcacat accggacagt cgtgcgggtg gttggtcatt aaccactcga
    11041 ccacactttc acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg
    11101 aagccggtgt catacaggac atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt
    11161 gcttcaccgc acactggcgg caagcaccga cgcttcccag cgccggatgc cagcaaaagt
    11221 aaggaatatc aaggcccaga gacagacaag cttccagcag gttgtccgct ccgttgacct
    11281 cgtattcttt gccgtctaca tgaattgtag ccattagcat gcttccagtt ttctcggtca
    11341 gggaccgagc gttaatcgaa attcggttac cagcgctctt tcagcaggtt cggctgaatc
    11401 ccattaatca aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg
    11461 aaatatttga tggcgctctg taacggctcc actgcaccag gtgcgtgggc acagaaagtt
    11521 ttacccgggc ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc cggctggcct
    11581 tcgccacgct ccagcgcacg cagaattttc acgctccacg gcagaccgtc gcggcacggc
    11641 gtacaccagc cgcaggactc acgggcgaaa aactcttcca ggttacgcac cagcgacacc
    11701 atgttgatct catggtcaac cgccatcgcc agcgccgtac ccagacggct gcccgcttta
    11761 ccgatacttt cgaattccat cggcagatca aggtgcgctt cggtcaggaa gtcagtcccc
    11821 gcgccgcctg gctgccaggc tttaaatttc agaccatcac gcataccacc ggcgtaatct
    11881 tcgaggatct cgcgtgcggt ggtgccgaac ggcagttccc acagtcccgg atttttcacc
    11941 cgaccggaga agcccatcag cttggtgcca gcatctttac ttttcgagat gttctgatac
    12001 cactccacgc cgttagcgag gatcgccgga acgttacaca gggtttcgac gttgttgaca
    12061 caggtcggtt tgccccatac gccggaggtt gccgggaagg ggggtttcga gcgtgggtta
    12121 gcacgacgcc cttccaggga gttgattaac gctgtttctt ccccgcagat gtagcgccct
    12181 gccccggtat ggacgaacag ttcgaagtcg aaacctgttc ccataatgtt tttgccaagc
    12241 aagcccgctt cagtggcttc ggcaatggcg cggcgcagat ttactgccgc ttcgatatat
    12301 tcgccacgca ggaagatgta gccacggtaa gctttcagcg caaacgcgga gatgagcata
    12361 ccttccacca gcaggtgcgg cagttgctcc atcaacaggc ggtctttata ggtgcccggc
    12421 tccatttcat cggcgttaca cagcaggtaa cggatgttca tggattcgtc tttcggcatc
    12481 aggctccact tcaggccagt cgaaaagccc gcgccgccgc gccctttcag accagcgtct
    12541 tttacctgat taacgatttc gtccggagac agcccggtca gcgccttacg cgccccttcg
    12601 taaccgtttt tgctgcggta ttcgtccagc cacactggct gtttgtcatc gcgcagacgc
    12661 caggtcagcg gatgcgtttc gggagtacgg ataatgtttt tcatttatac cgctccagca
    12721 gttcagggat cgcttccggg gtcagatgcg cgtgagtgtc ctcatcgatc atcatgtttg
    12781 gccctttatc acagttcccc aggcagcaag ttggcagcag cgtaaagcgg ccatcaaatg
    12841 tcgtttgccc tggtttgatg ttcagctttt tctcgagcgc cgcctgaata ccctgatacc
    12901 cgttgatatg acagaccacg ctgtcgcaat aacggatcac atggcgacca accggctggc
    12961 ggaagatctg actgtagaac gttgccacac cttcgacgtc gcttgccgga atacccagca
    13021 catcggcgat cgcgtggatc gcaccatccg gcacccagcc acgctgcttc tgaacgattt
    13081 tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg
    13141 cttcacgctc tgccgcactc agctcaaaag cctcggtttg tggttgttga ttctcgtgca
    13201 taattagcgg tccacatctg acataacaaa atcgatactg cccagataaa caatcaggtc
    13261 agacaccagg ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt
    13321 gcggatacga gtgcgataac tcatggtgct accgtcgctg gtcaggtagt aactgttgat
    13381 ccccttggtc gcctcaatca tctggaaaga ttcattggca ggcatcaccg gcccccacga
    13441 cacttgcagg aagtgggtga tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg
    13501 cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg
    13561 ctcaagaatg cgcagactct ggcgcagctc ttccactttc agcattacgc gggtgtagca
    13621 gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc cagaataagg
    13681 acgcgcctta cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc
    13741 ccactccagc gcctctttcg cgccataggc ggcaacgccc tgggaacgac ctttcagaat
    13801 ggtattttgc agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa
    13861 ctcacgcagc agacgatccc agccgcgcgg caggtcgtgc gctacgccgc caatacggaa
    13921 ccacgccggg tgcatacgga aacccgtgat cgcttccacc agatcgtaaa ttttctgacg
    13981 atcggtaaag gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat
    14041 gtacagcagg tggctgttga tacggaacag ttcggagagc ataacgcgaa tgacgttaac
    14101 gcgatccggc acggtgatcc cggccagttt ctctaccgcc agcacgtaag gcatttcgtt
    14161 aacgcagccg ccgaggtatt caatacggtc ggtatacgga atgtagctgt gccaggactg
    14221 gcgctcgccc attttctccg caccacggtg gtggtagccg atgtctggta cgcagtcgac
    14281 aatctcttcg ccgtcgagtt gtaaaacgat acggaaagcc ccgtgcgccg acgggtggtt
    14341 cggaccgagg ttgaggaaca tgaagtcctc attttcggtg ccgcgtttca tcccccactc
    14401 ttccggtttg aaggtcaggg cttccatctc cagatcctgt ttggctttgg tcagctcaaa
    14461 cggcgagaat tcggtagcgc gtgccgggta atctttacgc agcgggtgac ctttccaggt
    14521 ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg aaagtaatgc caaacagatc
    14581 ccaggtttca cgctcatacc agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg
    14641 caggtcgttt tctgccagcg ccaccttcag catgatgtcg cggttacgat cgatagaaat
    14701 cagatggtag aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg
    14761 ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt tctttaagaa
    14821 atcgccaact tccagtaatt gttcacgctt gatccacaca acgggaaccc cggtgcgagt
    14881 cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa ttaccggatc
    14941 atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcag tcatattgtt
    15001 caccattgca aatggtccgt ggtgactgtc ggcgcagtaa gcttcgcgaa atagatttag
    15061 gaataagcga agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt caggtgtacg
    15121 caggttggtt acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt gcatattggc
    15181 gcgataaacg ccctgatcgc caaccaccca ggagagcgga cgacgttctt tgccgataga
    15241 ttcctgcaac agcatcagtg cctgcatgta cgcttcagga cgcggcgggc agcccgggat
    15301 atacacatca accgggatga atttatcgac gccctgcaca acggaataaa tatcgtacat
    15361 accaccagag ttggcacagg cacccattga gataacccat tttggttcca gcatctggtc
    15421 atacagacgc tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag
    15481 gtcagcctga cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac
    15541 cgcggtaaac gaagtcacca tctcaacgta acagcaggaa agaccgaagt tatacggcca
    15601 aattgagttt ttacgacccc agttaaccat gtcattgagc ttgcccataa acacgttttt
    15661 gttaacttct tgctccagag ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc
    15721 gttctcaccg ttgggatcta tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta
    15781 gcgttgacga ttagcgatac tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc
    15841 gggcgtccag tccagcgcgc caatacgcac cagataaacc agacctgcca gtaacacaaa
    15901 aataaaaatt gcagcttcca caaagcctac ccagccgctt tcgcggatag aggttgacca
    15961 tgcgaacaga tacagcgctt caacgtcgaa gataacgaag aacatggcca ccagataaaa
    16021 cttggcggac aggcgtaagc gggcggagcc gaccgagtcg ataccggatt caaacggcac
    16081 gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca
    16141 acacaggcca atggcaacga taagaaagat agcgaatgcc cagtgatgag cgatgacttc
    16201 agtggatgtt gacatactca ttgcttactc atcaaaagta gcgccagatt cactgctctt
    16261 cacggcagat ggacgccaca tcgattcatg gggaggaata aaaaaaacct tacaataac
//
LOCUS       DAZPEU010000078        16130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPEU010000078 DAZPEU010000000
VERSION     DAZPEU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.078"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            116..1351
                     /gene="moeA"
                     /locus_tag="ACHU2L_002252"
     CDS             116..1351
                     /gene="moeA"
                     /locus_tag="ACHU2L_002252"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR1357092.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            1351..2100
                     /gene="moeB"
                     /locus_tag="ACHU2L_002253"
     CDS             1351..2100
                     /gene="moeB"
                     /locus_tag="ACHU2L_002253"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR1357093.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2279..2941)
                     /gene="fsa"
                     /locus_tag="ACHU2L_002254"
     CDS             complement(2279..2941)
                     /gene="fsa"
                     /locus_tag="ACHU2L_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1357094.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            3072..3971
                     /locus_tag="ACHU2L_002255"
     CDS             3072..3971
                     /locus_tag="ACHU2L_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR1357095.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3977..6409
                     /locus_tag="ACHU2L_002256"
     CDS             3977..6409
                     /locus_tag="ACHU2L_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR1357096.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6555..7370
                     /gene="ybiV"
                     /locus_tag="ACHU2L_002257"
     CDS             6555..7370
                     /gene="ybiV"
                     /locus_tag="ACHU2L_002257"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR1357097.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7522..8787
                     /locus_tag="ACHU2L_002258"
     CDS             7522..8787
                     /locus_tag="ACHU2L_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR1357098.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(9028..10620)
                     /locus_tag="ACHU2L_002259"
     CDS             complement(9028..10620)
                     /locus_tag="ACHU2L_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR1357099.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            10839..11759
                     /gene="ldtB"
                     /locus_tag="ACHU2L_002260"
     CDS             10839..11759
                     /gene="ldtB"
                     /locus_tag="ACHU2L_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1357100.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(11817..12935)
                     /locus_tag="ACHU2L_002261"
     CDS             complement(11817..12935)
                     /locus_tag="ACHU2L_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR1357101.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(12932..13399)
                     /gene="mntR"
                     /locus_tag="ACHU2L_002262"
     CDS             complement(12932..13399)
                     /gene="mntR"
                     /locus_tag="ACHU2L_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR1357102.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            13585..13713
                     /gene="mntS"
                     /locus_tag="ACHU2L_002263"
     CDS             13585..13713
                     /gene="mntS"
                     /locus_tag="ACHU2L_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR1357103.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            13985..15568
                     /gene="opgE"
                     /locus_tag="ACHU2L_002264"
     CDS             13985..15568
                     /gene="opgE"
                     /locus_tag="ACHU2L_002264"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR1357104.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(15616..>16130)
                     /gene="ompX"
                     /locus_tag="ACHU2L_002265"
     CDS             complement(15616..>16130)
                     /gene="ompX"
                     /locus_tag="ACHU2L_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR1357105.1"
                     /translation="KKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKMG
                     GFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASIY
                     GVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDVG
                     TWIAGVGYRF"
BASE COUNT         3924 a         4326 c         4109 g         3771 t
ORIGIN      
        1 gatggatatg gcatgtaaag gcaggagcca acgctacgcc cgaggtggca tacagtctat
       61 ggcgtttttc tgacataata ggcaaattcg attttgcctc cgcaggagtg ttttcatgga
      121 atttaccacc ggattgatgt cgctcgacac cgcgcttaat gagatgcttt ctcgcgttac
      181 cccactgacc acccaggaaa cgctgccact ggttcagtgt tttggtcgta ttctggcgag
      241 cgatgtcgtt tcgccactgg atgtcccggg gtttgataac tccgctatgg acggctacgc
      301 ggtgcgttta gccgatattg cctccgggca accgctgccc gttgccggta aatcctttgc
      361 cggtcagcca taccatggtg aatggcctgc gggtacctgc attcgtatta tgaccggtgc
      421 gccggtgccg gaaggctgcg aagcggtggt gatgcaggag cagactgaac aaacggacaa
      481 tggcgtgcgt tttactgctg aagtgcgtag cgggcaaaat attcgccgtc gcggtgaaga
      541 tatctctgca ggtgcggttg ttttcccggc gggaactcgc ctgactaccg cagagctgcc
      601 agtaattgct tcactgggga ttgccgaagt tccggtgatt cgtaaagtgc gtgtagcgct
      661 tttttctacc ggtgatgaac tccagttgcc cggtcagccg ctgggcgacg gccaaatcta
      721 cgacaccaac cgtctcgccg tacacctgat gttagaacag ctgggttgcg aggtaatcaa
      781 cttagggatt atccgcgacg atccacatgc cctgcgcgcc gcatttattg aagccgacag
      841 ccaggcggat gtagtgatca gttccggcgg tgtttcagtg ggtgaagcgg attacaccaa
      901 aatgattctt gaagagctgg gggagatcgc cttctggaag ctggcgatta aaccaggtaa
      961 accgttcgcg ttcggtaaac tcagcaatag ctggttctgc ggcctgccgg gcaacccggt
     1021 ttcagcgacg ctgaccttct atcaactggt acagcctttg ctggcaaaac taagcggcaa
     1081 caccgccagc ggcctgcccg cgcgccagcg cgtacgcaca gcgtcccgcc tgaaaaaaac
     1141 gccaggacgt cttgatttcc agcgcggcgt gctgcaacgc aacgccgacg gtgaacttga
     1201 agtgacgacc accggacatc agggttcaca tatatttagc tcctttagtc tcggcaactg
     1261 ctttatcgtg ctggaacgcg atcgcggcaa tgtggacgtg cgcgaatggg tggaagtaga
     1321 accgtttaac gcgttgttcg gaggcctgta atggcggaac tcagcgatca ggagatgctg
     1381 cgctacaacc ggcaaatcat tctgcgcggt tttgattttg acggtcagga ggcgctaaaa
     1441 gattctcgtg tgctggtagt cggcctcggt ggactcggct gcgcagcttc gcagtatctg
     1501 gcaagcgccg gtgtcggcaa cctgacgctg ctcgacttcg acacggtttc gctctcgaat
     1561 ctgcaacgcc agacactgca cagtgatgcc acggtcgggc aaccgaaggt ggaatccgcc
     1621 cgtgacgccc tgacgcggat caacccacat atcgcgatta cgccagtcaa tgcactgcta
     1681 gatgacgcag aacttgcagc attgattgct gaacacgatc tggtgctcga ctgtacggat
     1741 aacgttgcgg tacgtaatca actgaacgca ggctgttttg ccgcgaaggt gccgctggtt
     1801 tccggcgcgg caattcgtat ggaagggcaa atcacggtct tcacttatca ggacggcgaa
     1861 ccgtgctatc gctgccttag ccgtttgttt ggcgagaacg cgttaacctg cgtagaagca
     1921 ggcgtgatgg caccgttgat aggcgtaatt ggttcgttgc aggcaatgga agcgatcaaa
     1981 ctgctggcag gttatggcaa accggccagc gggaaaatcg tcatgtacga tgcgatgacc
     2041 tgtcagtttc gcgaaatgaa actgatgcgc aatccggggt gtgaggtgtg cgggcagtaa
     2101 ttgccacctc caagcctgat aagacgcgac aagcgtcgca tcaggcattt gctccagacg
     2161 ccagatgcgg cgtgaacgcc ttatctggcc tacggttcgg gcacagattt ccaagcgcgg
     2221 caggcatcgg tgcactccgc cacgtcatca gcaatttatg aggatgcaga acgtccggtt
     2281 aaatcgacgt tctgccaaac gctccctgcc agtcctgctc aaacttcgtc acagcggctt
     2341 ccaccgccgg atagctaatc atctgttgtg ccacatccag tggcagagta attgattcac
     2401 atccggccag taagcagtcc agcgcctgac gcggggtttt gaaactcgct gccagcactt
     2461 tcgcctgcgg cgcatgcatt ttcaataact ggtgtaagtc ggtcacagtc tgaatgccgc
     2521 taccgccctg agcatcaata cgattaacgt aaggcgcaac atattccgca cctgccagcg
     2581 ccgacagcag cccttgtgcc gcgccatata ccgcggttcc cagcgtcgga atcccttccg
     2641 cttttaacat cttaatcgct gccagcccct cggctgtcac cggaactttc accacgatat
     2701 ccgcaataat agaacgcagc ttaagcgcgt cattcaccat cccttcagca gtggtagcca
     2761 ttacctgggc aaacagacgc ccctgaccgc ccatcgcttc atgaagttgc ggaagcacaa
     2821 catccagcgg ttttttaccc gcggcgataa tgcttgggtt agtggtcaca cccgccagcg
     2881 gaaaaatacg tgacagcgcc ttcaccgcaa caacgtctga agtatccaga tacagttcca
     2941 taaccatcct caaaatgtac tttttgtctg aatgtagact atcacgggaa aactctctga
     3001 acagttgacg cacatcaatc taactttcat tcgaaagtat tttaatgttt gaacgaaaca
     3061 gagaggcaac tatgattttc aatattcagc gctactcgac ccatgatggc cccggtatcc
     3121 gcacggtcgt atttcttaaa ggctgttcgc tgggctgccg ctggtgtcag aacccggaaa
     3181 gccgcgcccg tacgcaggat ctgctgtatg acgcacgact gtgtctggaa ggctgcgagc
     3241 tgtgcgctaa ggccgcgccg gaagtgattg agcgcgcgct gaatggtttg cttattcatc
     3301 gggaaaagtt aaccccggag catctgacgg cgttaaccga ctgctgtccg acacaggcgt
     3361 taaccgtgtg tggtgaagtg aaaagcgttg aggagatcat ggcgaccgtt ctgcgcgata
     3421 aaccgtttta cgatcgcagc ggcggcggct taacgctttc gggtggtgaa cccttcatgc
     3481 agccggaaat ggcgacggcg ctgctgcaag cctgccacga ggcaggcatt catactgcgg
     3541 tagaaacctg tctgcatgtg ccgtggaaat atatcgcccc ttctctgccc tatatcgatc
     3601 tgtttcttgc cgatttaaaa cacgttgccg acgcgccgtt taaacagtgg accgacggta
     3661 acgccgccag ggtgctggat aacctgaaaa aactcgccgc agcgggcaaa aaaatcatta
     3721 tccgcgtgcc gctgattcag ggctttaatg ccgacgaaac ctctgtaaaa gccattaccg
     3781 attttgccgc cgacgagctg cacgttggcg aaattcattt tctgccctac cacacgctgg
     3841 gcatcaacaa atatcactta cttaatctgc cctatgacgc cccggaaaaa ccgcttgatg
     3901 cgccagagct gctcgatttt gcccagcagt atgcctgcca gaaagggtta accgcgacct
     3961 tacgaggata acaaccatga ccacactgaa actggacacg ctcagcgacc gcattaaagc
     4021 gcacaaaaat gcgctggtgc atattgtgaa gccgccagtc tgtaccgagc gcgcgcagca
     4081 ctataccgag atgtatcaac aacatctcga taagccgatc ccggtacgtc gcgcgctggc
     4141 actggcgcat cacctggcga atcgcaccat ctggatcaaa cacgatgagt tgatcattgg
     4201 caaccaggca agcgaagttc gcgccgcgcc gatcttcccg gaatatactg tctcgtggat
     4261 cgaaaaagag attgatgatc tggcagatcg tcccggtgct ggctttgcgg tgagcgaaga
     4321 gaacaaacgt gttctgcatg aagtgtgccc gtggtggcgc ggtcagacgg tacaggatcg
     4381 ctgctatggc atgtttaccg atgagcaaaa aggtctgctg gcgaccggca tcattaaagc
     4441 ggaaggcaat atgacctccg gcgatgcgca cctggcggtc aatttcccgc tgctgctgga
     4501 aaaagggctt gatggtctgc gtgagaaagt agcggaacgt cgctcgcgca tcaatctgac
     4561 ggtgctggaa gatttgcacg gcgaacagtt cctgaaagcg attgatatca tgctggtggc
     4621 agtcagtgaa cacattgaac gtttcgctgc cctggcgcgt gcaatggccg cgaccgaaac
     4681 ccgcgaaagc cgtcgcgatg aactgctggc gatggcagaa aactgcgatc ttatcgccca
     4741 ccagccgccg cagactttct ggcaggcgct gcaactgtgt tacttcatcc agttgatttt
     4801 gcagattgaa tctaacggtc actcggtgtc gtttggtcgt atggatcagt atctctaccc
     4861 gtactatcgc cgcgacgttg aactgaacca gacgctggat cgcgaacacg ccatcgaatt
     4921 gctgcatagc tgctggttga aattgctgga agtgaacaaa atccgctccg gctcccactc
     4981 aaaagcctct gcgggaagtc cgctgtatca gaacgtcacc attggcggac aaaatctggt
     5041 tgatggtcaa ccaatggacg cggtgaatcc actctcttac gcgatcctcg aatcctgcgg
     5101 tcgcctgcgt tccactcagc ctaacctcag cgtgcgttac catgcaggaa tgagtaacga
     5161 tttcctcgac gcctgcgtac atgtgatccg ctgcggcttc gggatgccgg cattcaacaa
     5221 cgacgaaatc gtgatcccgg aatttattaa actcggtatt gaaccgcagg acgcttatga
     5281 ctacgcagcg attggttgta tagaaaccgc cgtcggtggc aaatggggct atcgctgtac
     5341 cggcatgagc tttatcaact tcgcccgcgt gatgctggcg gcgctggaag gcgggcgtga
     5401 tgccaccagc ggcaaagtgt tcctgccaca agaaaaagcg ttgtcggcag gtaacttcaa
     5461 caacttcgat gaagtgatgg acgcgtggga tacgcaaatc cgttactaca cgcgtaaatc
     5521 aatcgaaatc gaatatgtcg tcgacaccat gctggaagag aacgtgcacg atattctctg
     5581 ctcggcgctg gtggatgact gtattgagcg agcgaaaagt atcaagcaag gcggcgcgaa
     5641 atatgactgg gtttctggcc tgcaggtcgg tattgccaac ctcggcaaca gcctggcggc
     5701 agtgaagaaa ctggtgtttg aacagggcgc gattggtcag caacagctgg ctgccgcact
     5761 ggcggatgac ttcgatggcc tgactcatga gcaactgcgt cagcggctga ttaacggtgc
     5821 gccgaagtac ggcaacgacg atgataccgt cgatacgctg ctggctcgcg cttatcagac
     5881 ctatatcgac gaactgaaac agtaccataa cccacgctac ggtcgtggtc cggttggcgg
     5941 caactattac gcgggtacgt cgtctatctc cgctaacgta ccgtttggcg cgcaaactat
     6001 ggcaacgccg gacgggcgta aagcccacac cccgctggca gaaggcgcta gtccggcctc
     6061 tggtactgac catctcggcc ctactgcggt cattggctca gtgggtaaac tgcctacggc
     6121 agcgattctc ggcggcgtgt tgctcaacca gaaactgaat ccggcaacgc tggagaacga
     6181 atctgacaag cagaaactga tgatcctgct gcgtaccttc tttgaagtgc ataaaggctg
     6241 gcatattcag tacaacatcg tttcccgcga aacgctgctg gaggcgaaaa aacatccgga
     6301 tcagtaccgc gatctggtag tgcgtgtcgc gggttattcc gccttcttca ccgcgctctc
     6361 tccagacgct caggacgata tcatcgcccg tactgaacat atgctgtaat ctcttcggcc
     6421 cggctgcggc gtcgccgggc taaatcattc acatcatcaa tttcatcctt actttcattc
     6481 gaaatataat ttgtgctctg cgtcacattg ttattacact gttacggttt cattttcgag
     6541 ccaggagcac acctatgagc gtaaaagtta tcgtcacaga tatggacggt acttttctta
     6601 acgacgccaa aacgtacaac caaccacgtt ttatggcgca atatcaggaa ctgaaaaagc
     6661 gcggcattaa gttcgttgtc gccagcggta atcagtatta ccagcttatt tcgttctttc
     6721 ctgagctaaa ggatgagatc tcttttgtcg cggaaaacgg cgcactggtt tacgaacatg
     6781 gcaagcaact gttccacggc gaactgaccc gacatgaatc gcggattgtt attggcgagt
     6841 tgctaaaaga taagcaactc aattttgtcg cctgcggtct gcaaagtgca tatgtcagcg
     6901 aaaatgcccc cgaagcattt gtcgcactga tggcaaaaca ctaccatcgc ctgaaacctg
     6961 taaaagatta tcaggagatt gacgacgtac tgttcaagtt ttcgctcaac ctgccggatg
     7021 aacaaatccc gttagtgatc gacaaactac acatagcgct ggatggcatt atgaaacccg
     7081 tcaccagtgg ttttggcttt atcgacctga ttattcccgg tctacataaa gcaaacggta
     7141 tttcgcggtt actgaaacgc tgggatctgt caccgcaaaa tgtggtagcg attggcgaca
     7201 gcggtaacga tgcggagatg ctgaaaatgg cgcgttattc ctttgcgatg ggcaatgctg
     7261 cggaaaacat taaacaaatc gcccgttacg ctaccgatga taataatcat gaaggcgcgc
     7321 tgaatgtgat tcaggctgtg ctggataaca catccccttt taacagctga cctctctacc
     7381 ggagccatgc gctccggttt tcccttcttt ttacatcctg tacatcacat caaatcatta
     7441 aacttgcatt aacttattta taacttgtat tgtcacatgc aaattagcat agtttcgaat
     7501 gaaagttaat gcaaggtggt tatggcttct acttttacca gcgacacatt gcctgccgat
     7561 cacaaagcag ctatccgtca gatgaagcac gcgctgcggg cgcagcttgg cgatgtccag
     7621 cagatcttta atcagctaag cgatgacatt gccacgcgag tggctgaaat caacgcactc
     7681 aaagcacagg gcgatgccgt ctggccggtg ctgtcttatg ccgatatcaa agcaggtcat
     7741 gttactgcag agcagcgcga acagattaaa cgtcgcggtt gtgcggtgat aaaaggccat
     7801 ttcccccgcg aacaagcgct aggctgggat cagtcgatgc tggactatct gaaccgcaac
     7861 cgctttgacg aggtctacaa aggccccggc gataatttct tcgggacgct cagcgcttca
     7921 cgtcccgaga tttaccccat ctactggtcg caggcgcaaa tgcaggcccg ccagagtgaa
     7981 gaaatggcga atgcgcagtc gtttctcaat cgtctgtgga catttgaaag tgatggaaag
     8041 caatggttta acccggatgt gagcgtcatc taccctgacc gtatccgccg ccttccgccc
     8101 ggaacgacct ccaaaggtct tggagcgcat accgactccg gggcgctgga acgctggctg
     8161 cttccagcgt atcagcacgt tttcgccaac gtctttaatg gcaatctggc gaaatacgat
     8221 ccctggcatg cggcacatcg tacggaagtt gaagagtaca cggtggacaa caccaccaaa
     8281 tgttccgtgt ttcggacatt ccagggctgg actgcgctct cggatatgct gcctggtcag
     8341 gggttgctgc acgtcgtgcc cattcctgaa gccatggcgt acgtactgtt acgtccgctg
     8401 cttgatgatg tgccggagga tgaactgtgc ggcgtagcgc ccggaagagt gttgccggta
     8461 tcagagcaat ggcatccact gttaattgag gcgttaacca gcattccaaa actcgaggcc
     8521 ggagactccg tctggtggca ctgcgacgtc atccattccg ttgcccccgt tgaaaatcaa
     8581 cagggttggg gcaacgtgat gtacattcct gcggcaccga tgtgcgagaa aaatcttgcc
     8641 tacgcgcaca aggtgaaggc cgcactggaa aaaggcgcat cgccgggcga cttcccgcgc
     8701 gaggactatg aaacaaactg ggaaggacgc tttacgcttg ccgacctcaa cattcacggt
     8761 aagcgagcgt tgggcatgga tgtttgagag ttaatggaag tgaattgatg tgggattaag
     8821 aaaatcgatt cgacgttact cagattgctg acaagtgcgc tttcttaatg cctgatgcgg
     8881 cgttaacgcc ttatccggcc tacaaagtcc tgcagattca atatattaca ggtactgcgt
     8941 aggcctgata agcgtagcgc atcaggctat tttgcatttg tcatcagccg tcgtcacccc
     9001 gacagtatcg gggtgacgga taatcactta ctcgatccct ttactacgca ggtaatcttc
     9061 gtaattaccg ctaaagtcga tcacgcgttc cggggtgatt tccagaatgc gggtcgccag
     9121 ggagcttacg aactcacggt cgtgtgaaac aaagatcagc gtgccctgat acagttccag
     9181 cgccatgttc agtgactcga tggattccat atccaggtgg ttggtcggtt cgtccatgat
     9241 cagaatgttc ggcttctgca tcattaactt accaaacagc atccgccctt tttccccacc
     9301 ggaaagcact ttagctggct ttttgatgtc gtcctggctg aacagcaaac gaccgagaat
     9361 gctgcgtacc gcctgctcgt catcgccttc ctgcttccac tgactcatcc attcgaacac
     9421 ggtcagatca ttttcaaact catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt
     9481 ttcagaccat tttacggtgc cgctgtccgg ttgcagatcg cccaccagcg ttttcagcag
     9541 cgttgattta ccgacgccgt tggtacccag taccgcaagt ttttcaccga cctccagcag
     9601 caggttgaga tttttaaaca gtggaccgtt atcaaaccct ttggtcagac cttccacttc
     9661 cagcgcgtta cggaacagtt tcttatcctg ttcaaaacgg ataaacgggt tctgacggct
     9721 ggaggctttc acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg
     9781 gcgagatttc gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact cagcaatctg
     9841 cgctttcttc ttggcgttat cggccagcag acgttcacgc gcttgggtcg ccgccgtcat
     9901 gtactcatcg tagttacccg gataaacgcg cagctcgccg taatccagat ccgccatgtg
     9961 ggtacagacc atgttaagga agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg
    10021 ctcgttcagc acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc
    10081 gtcgagcagg agaatatccg gctcagcaaa cagcgcctgc gccagaagca cacgcagctt
    10141 ccagccagga gcaacttcac tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc
    10201 aagcaacagt tcaccggcgc gagcttccgc agagtaaccg tccatttcgc cgtatttcac
    10261 ttccagatcg gccactttat agccgtcttc ttcactcatt tccggcaaag catagatgcg
    10321 gtcgcgctcc tgcttcactt cccacaactc tttatgcccc atgatcaccg tatccagcac
    10381 agtgaactct tcaaaggcaa actgatcctg acgcagctta ccaatgcgct cgttgggatc
    10441 gagggaaacg ttacccagcg tcggctcaag gtcgccgccg agaatcttca taaaggtgga
    10501 tttaccacta ccgttcgcgc caatcaggcc gtaacggttg ccgccgccga atttgacgga
    10561 aatgttttca aacaacggct tactgccgaa ctgcatggtg acgttactgg aaactaacac
    10621 aggcgtatcc tgaaaagaga tatgacaaac cgcgcattat gccataagtc tgaaagaaga
    10681 tcctgctcct cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga tttcgtacac
    10741 atctgatttc actgtgagct ggaatgaact tataatgcgc ttccaatact ctaatattct
    10801 caacccaatg gcctgccagg cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat
    10861 tattcgcagc ggccttcgct gttgtcggct tttgcagtac cgcctctgcg gtaacttatc
    10921 ctctgccaac cgacgggagt cgcctggttg gtcagaatca ggtgatcacc attcctgaag
    10981 gtaacactca gccgctggag tattttgccg cggagtacca gatggggctt tccaatatga
    11041 tggaagcgaa cccgggtgtg gataccttcc tgccgaaagg cggtactgta ctgaacattc
    11101 cgcagcagct gatcctgccg gataccgttc atgaaggcat cgtcattaac agtgcagaga
    11161 tgcgtctgta ttactatccg aaagggacca acaccgttat cgtgctgccg atcggcattg
    11221 gtcagttagg caaagatacg cctatcaact ggaccaccaa agttgagcat aagaaagcag
    11281 gcccgacctg gacgccgacc gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc
    11341 taccggctgt cgttccggca ggtccggata acccgatggg tctgtatgca ctctacatcg
    11401 gtcgcctgta tgctatccat ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc
    11461 atggttgtgt gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg
    11521 gtacccgcgt acagtttatt gatgagccgg taaaagcgac caccgagcca gacggcagcc
    11581 gttatattga agtccataac ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa
    11641 tcgtgccaat taccctgacg aagagcgtgc agacagtgac cggtcagcca gatgttgacc
    11701 aggttgttct tgatgaagcg atcaaaaatc gttccgggat gccggttcgc ctgaattaat
    11761 cgtcaaaacg taaaacaaag gcggactcat aatccgcctt ttttatttgc cagaccttac
    11821 caggccggga tcataactaa caaaacgtat cccaccagcg ccgcccacaa cagcatcggt
    11881 attgaataga gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc
    11941 aaatttgcca gcgatcctgg taataaacca aagcccccca cgtttaccgc ccatgccagt
    12001 aataaagacg gaggtacata attgagcagc aaaatggtac tcggcacgtt actgatcacc
    12061 tgcgataaac cgactgccgt taaccataac ccgggttcag ataaatgact cacgttaccc
    12121 aacacgcctt gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata
    12181 aacaccagca gcaacgtcca gtccacactc aacaccacgc gacgcgccag cagcacaaag
    12241 cccgccgcca caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag
    12301 acgatataca gccccagaca attccacacc agccgcggtt tccactccgg tgtttgcacc
    12361 cccgtatggt attgcagcgc ctttccaggg aaacaacacc agcacaacag caggagtgtc
    12421 agcatcattg cgccagccat cggtgccatt tgggcaataa atccggcaaa cgaaagacca
    12481 gaacgtcccc agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg
    12541 ttgactgcca gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt
    12601 agcgtgatag tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa
    12661 agcagcgccg ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc
    12721 accattttgc gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc
    12781 atcaggccgc ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt
    12841 gcaaagggca cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga
    12901 tcgccttgca gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca
    12961 aacgaaaagc gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac
    13021 gggcgatttc cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct
    13081 cgcggctttc ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga
    13141 tcatttcaat cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg
    13201 aaactcccag acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt
    13261 cagaaatcag ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc
    13321 ggaacccttc aacgtgctct tccacgttca ctaactgcgt cacttttttt gctattggcg
    13381 tacctgcgcg acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat
    13441 ttcgtgcgca cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata
    13501 gctatataat atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac
    13561 gggattagca agtcaggagg tcttatgaat gagttcaaga ggtgtatgcg cgtgtttagt
    13621 cattctccct ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac
    13681 aaaccgcagc aagataaacc ttcagataaa tagcggcgtc gcggtacgcc gcttcactcc
    13741 tgcttttatg caggcataac gcgttttggt ctgaaaaaaa ccactttttg tcggatttgc
    13801 aatccccttc gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc
    13861 ttcttgcagc cctgcgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc
    13921 actcttgcag gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg
    13981 gcatatgaat ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg
    14041 gactgcgttc tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag
    14101 tttgctctac accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg
    14161 cgtacaaaaa gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc
    14221 tcaggcctac ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga
    14281 agagtcgacc gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat
    14341 ttttgcgctc ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa
    14401 taccttcgac agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca
    14461 aaacctggcc tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt
    14521 tgctaaggat gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa
    14581 actttccgga atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta
    14641 tgtggtagtg atcggtgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg
    14701 ggacaatacc ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgctgc
    14761 cagtggctcc acgcagaaat cgcttggctt aacgctcaat cgcgtagtcg atggcaaacc
    14821 acagtttcag gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt
    14881 ttccaaccag ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc
    14941 agatgaagtg tacttcctga aagaaggtaa ttttgaagca gataaaaaca cgaaagacga
    15001 agcgttactg gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat
    15061 tgttctgcat ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga
    15121 aacctttgtg caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga
    15181 tttactgcgc aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta
    15241 cttttctgac cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca
    15301 tgatgataaa tatcagcaaa atttccaggt gccttttatg gtcatttcca gcggcgataa
    15361 agcgcatcgg gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca
    15421 gtggacggga attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa
    15481 agccgggccg atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg
    15541 aaccgatatt ttcgacccga aaccttaaaa acaaaaatcc gccccgagag gcggattttt
    15601 atatcaccaa agtgattaga agcggtaacc aacaccggca atccaggtgc ctacgtcaac
    15661 gctacgaata cggctctgct cgtaagagaa gtccagagca acgttttcca tcgggttgaa
    15721 ctgcagacca gcaccgtagg agaaaccgta gtcgctggtg tcgtgtttgt aggtcgggta
    15781 ttcagtggtc tggaatttac cataacccac acccactaca ccgtagatgc ttgcccagtc
    15841 gttaatgcgg taagccggac cagcagtgat gccgtagtac tggtttttgt tgtagtcacc
    15901 agagcttgca gtacggcttt tctcggtgta agtgaaagaa ccgatcacac ccagcgggct
    15961 gttgtcttct tcatagcggt atttcaggtt gaaaccgccc attttgttca tttggccctg
    16021 agcgtcgctc tgtgcgtaac cgccagttac ggtagaagtc gcagctacgg aagtacctgc
    16081 ggtgaaagcc agaactgcgg ccagtgctga aagacatgca atttttttca
//
LOCUS       DAZPEU010000079        15982 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPEU010000079 DAZPEU010000000
VERSION     DAZPEU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.079"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(103..1557)
                     /gene="xylB"
                     /locus_tag="ACHU2L_002266"
     CDS             complement(103..1557)
                     /gene="xylB"
                     /locus_tag="ACHU2L_002266"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR1357106.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1629..2951)
                     /gene="xylA"
                     /locus_tag="ACHU2L_002267"
                     /pseudo
     CDS             complement(1629..2951)
                     /gene="xylA"
                     /locus_tag="ACHU2L_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            3317..4309
                     /gene="xylF"
                     /locus_tag="ACHU2L_002268"
     CDS             3317..4309
                     /gene="xylF"
                     /locus_tag="ACHU2L_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1357107.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4387..5928
                     /gene="xylG"
                     /locus_tag="ACHU2L_002269"
     CDS             4387..5928
                     /gene="xylG"
                     /locus_tag="ACHU2L_002269"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR1357108.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5906..7087
                     /gene="xylH"
                     /locus_tag="ACHU2L_002270"
     CDS             5906..7087
                     /gene="xylH"
                     /locus_tag="ACHU2L_002270"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR1357109.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7165..8343
                     /gene="xylR"
                     /locus_tag="ACHU2L_002271"
                     /pseudo
     CDS             7165..8343
                     /gene="xylR"
                     /locus_tag="ACHU2L_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(8451..9275)
                     /locus_tag="ACHU2L_002272"
     CDS             complement(8451..9275)
                     /locus_tag="ACHU2L_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR1357110.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9595..11625
                     /gene="malS"
                     /locus_tag="ACHU2L_002273"
     CDS             9595..11625
                     /gene="malS"
                     /locus_tag="ACHU2L_002273"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1357111.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11803..13056
                     /gene="avtA"
                     /locus_tag="ACHU2L_002274"
     CDS             11803..13056
                     /gene="avtA"
                     /locus_tag="ACHU2L_002274"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR1357112.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13207..13680)
                     /locus_tag="ACHU2L_002275"
     CDS             complement(13207..13680)
                     /locus_tag="ACHU2L_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1357113.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(13782..14630)
                     /gene="yiaJ"
                     /locus_tag="ACHU2L_002276"
     CDS             complement(13782..14630)
                     /gene="yiaJ"
                     /locus_tag="ACHU2L_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR1357114.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            14830..15828
                     /gene="yiaK"
                     /locus_tag="ACHU2L_002277"
     CDS             14830..15828
                     /gene="yiaK"
                     /locus_tag="ACHU2L_002277"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR1357115.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15840..>15982
                     /locus_tag="ACHU2L_002278"
     CDS             15840..>15982
                     /locus_tag="ACHU2L_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR1357116.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         4072 a         4062 c         3915 g         3933 t
ORIGIN      
        1 attaacaaat gattttcaga ataaattcat actaaatatt aattaattag tgagatatat
       61 agatgtgaat tatcccccac ccggtcaggc aggggataac gtttacgcca ttaatggcag
      121 aagttgctga tagaggcgac ggaacgtttc acgtcgtggc tgataagcgg cataacgctg
      181 cgcatctggt agatgcgact gttctaacgg tagctgcggc aacaactcaa tgagcgattt
      241 ctctggattc gccgcaatct gcgccagcct tgctgcgccc agtgctggcc ccacatcccc
      301 ccccgtacgg taatcgagct gctgaccgct gatatccgcc agcatctgac gccagtactc
      361 actacgcgcc ccgccaccaa tcaacgtaac actttgcggt ttaatgccgc aggcatgcac
      421 gacatccatg ccatctgcca gcgcataacc cacgccttcc agcactgctc gcgccagttc
      481 attggggcca tgttgatgag tcaaaccaaa gaaaaccccc ttcgcctggg gattattgtg
      541 tggtgtacgc tcgccggaaa gataaggcag aaaccaaact ggctcggcac tttcatcagc
      601 ctgttgagct gcagcgatta aagctgggac attgcacagg ccggttaatt tcgcggccca
      661 atccagacac gatgctgcac tcagcattac agacattaaa tgccaacgct gcggtagcgc
      721 atggcaaaag ctgtgtacag cgctttctgg cttgcttaag aacccttcgc tgacagcaaa
      781 atagaccccc gacgtcccca gcgataacat tgcctgatta gcatcaacca ttcccacacc
      841 aactgcacca gctgcattgt cgccaccgcc tgcgacaact ggcaccgtcg ccatacccca
      901 cgctttcgca acttcaggta acaaagcacc agtaatttcg ctgccttcgt ataatgcggg
      961 catctggtca cgagataagt cgcaagcctg cagcatgacg tcactccagt cacgctttgc
     1021 gacatccagc cacatggtgc cagctgcgtc agacatatcg ctggcaaact cccccgtcat
     1081 acgcagacgc aagtaatctt tcggtaataa tactttgtcg atttgacgga atatctccgg
     1141 ctcatgccgc tgaacccata gcaatttagg cgcagtaaat ccgggcatca tcaggttgcc
     1201 ggtaatcact cgtgattgcg gaactctcgc ttccagcaaa gtgcactctt gcgcacagcg
     1261 cccgtcgttc cacaaaatgg cagggcgcaa tacccgttgt tgagcatcca gtaaggttgc
     1321 tccatgcatc tggccggcaa tacccaatgc tttaacgtcc tgcagagaat gctgatcgcc
     1381 cagagctttc attgcgcgat cagttgcctg ccaccactgt tccgggtctt gttccgacca
     1441 aagtggatgc gggcgcgaaa cggtcagctt ttccgtttgc gaagcaacca cctcaccctg
     1501 ctcgttgagc aaaataactt ttacgcccga ggtgccaaga tctatcccga tatacatatc
     1561 gatcgttcct taaaaaaatg cccggtatcg ctaccgataa ccgggccaac ggactgcaca
     1621 gttagccgtt atttgtcgaa cagataatga tttaccagat tttccagttg ctcctggcga
     1681 ccactctgat gcaccggaga caaattatgt tcctgagcat atttggctaa atctgccagt
     1741 gacatttggc ctttcaggat ttgctggccc aattcgctat tccagccgga ataacgctgc
     1801 gcgatgcgtt tatccagctc gccatcttca atcatgcgcg ctgcaatttt cagcgccagt
     1861 gccatcgtat ccatcgcgcc gatatgaccg taaaacagat catatttatc agtactttga
     1921 cgacgtactt tggcatcgaa gttcagacca ccggtggtga aaccgcctgc tttgagaatt
     1981 tcatacatca ccagcgcatt ctcttccaca ctgttcggga actggtcggt gtcccagccc
     2041 agttgcgcat cgccacggtt ggcgtcgaca gaaccgaaca ggccaagcgc aatggcggtg
     2101 gctatttcat gatggaaaga gtgacctgcc agcgtcgcgt ggttagcttc aatgttcagt
     2161 ttaatctctt tttccagacc aaactgtttc aggaagccat agaccgtcgc ggcatcgtaa
     2221 tcatattgat gtttagtcgg ttcttgcggt ttcggttcga taagcaacgt gccctagaag
     2281 ccgattttat gtttatgctc aaccaccatc tgcataaagc ggcccagttg ttcacgctcc
     2341 tgacgcaagt cggtatttaa cagcgtttcg taaccttcac gaccacccca caggacatag
     2401 ttttcaccgc ccaatttatg ggttgcttcc atcgctgtaa caacttgcgt tgccgcccag
     2461 ctgaagactt caggatctgg gttcgtcgcc gcacccgcgc catagcgagg gtttgtaaag
     2521 cagttagcgg ttccccacag cagcttcacg ctgctctctt cttgcttggc tgccaggaca
     2581 tcaaccattt gcgcaaaatt attgatgtac tcttttaacg acgcgccctc aggggaaaca
     2641 tccacatcgt ggaagcaata aaatggcaca tgtaacttgt ggaaaaactc aaatgcgaca
     2701 tctgctttac gcttcgccaa cgccagtgcc tcaccaggct gctgccacgg acgattaaac
     2761 gcccccacac caaacatgtc cgccccgttc cagcagaagg tgtgccagta gcaggcggca
     2821 aaacgcaagt gctcttccat acgcttaccc aacaccagtt cgtcgggatt gtagtgacgg
     2881 aatgctaacg ggtttgagga ttttgagcct tcataacgaa cgcgatcgag ctggtcaaaa
     2941 taggcttgca tattgaactc cataatcagg taatgccgcg ggtgatggat gatgtcgtaa
     3001 tattgggcac tccctttcaa ttgctcaatt atgttatttc acactgctat tgagataatt
     3061 cacaagtgtg cgctcgctcg caaaataaaa tgggatgatg aaaatgggta attcctcgaa
     3121 gataaaaatg caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga
     3181 aataaaccaa aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt
     3241 gctaaaaatt ggttacgttt atcgcggtga ttgttactta ttaaagctgt cctctaacta
     3301 cagaaggccc tacaccatga aaataaagaa cattctactc accctttgca cctcacttct
     3361 gcttaccaac gttgcggcac acgccaaaga agtcaaaata ggtatggcga ttgatgatct
     3421 ccgtcttgaa cgctggcaaa aagatcgaga tatttttgtg aaaaaggcag aatctctcgg
     3481 tgcgaaagta tttgtacagt ctgcaaatgg caatgaagaa acacaaatgt cgcagattga
     3541 aaacatgatt aaccggggtg tcgatgttct tgtcattatt ccgtataacg gtcaggtatt
     3601 aagtaacgtt gtaaaaaaag ccaaacaaga aggtattaaa gtattagctt acgaccgtat
     3661 gattaacgat gcggatatcg atttttatat ttctttcgat aacgaaaaag tcggtgaact
     3721 gcaggcaaaa gccttggtcg atattgttcc gcaaggtaat tacttcctga tgggcggctc
     3781 gccggtagat aacaacgcca agctgttccg cgccggacaa atgaaagtgt taaaacctta
     3841 cgttgattct ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct ggttaccgga
     3901 aaacgcattg aaaattatgg aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt
     3961 agttgcctca aacgatgcca ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt
     4021 atcagggaaa gtagcaattt ccggccagga tgcggatctc gcaggtatta aacgtattgc
     4081 tgccggtacg caaactatga cggtgtataa acctattaca ttgctggcaa atactgccgc
     4141 agaaattgcc gttgaattgg gcaatggtca ggagccaaaa gcagatacct cactgaataa
     4201 tggcctgaaa gatgtcccct cccgcctcct gacaccgatc gatgtgaata aaaacaacat
     4261 caaagatacg gtaattaaag acggattcca caaagagagc gagctgtaag cgttacgccc
     4321 cggcgcggag cgggggcgtg atttctctcc atgccgcgtg aatgaattgg cttaggtgga
     4381 gtcgttatgc cttatctact tgaaatgaag aacattacca aaaccttcgg cagtgtgaag
     4441 gcgattgata acgtcagctt gcggttgaat gctggcgaaa tcgtctcact ctgtggggaa
     4501 aatgggtctg gtaaatcaac gctgatgaaa gtgctgtgtg gtatttatcc ccatggctcc
     4561 tacgaaggcg aaattatttt tgcgggagaa gagattcagg cgagtcacat ccgcgatacc
     4621 gaacgcaaag gtatcgccat cattcaccag gaattggccc tggtgaaaga attgaccgtg
     4681 ctggaaaata tcttcctggg taacgaaata acccacaatg gcattatgga ttatgacctg
     4741 atgacgctac gctatcagaa gctgctcgca caggtcagtt tatccatttc acctgatacc
     4801 cgcgttggcg atttagggct tgggcaacaa caactggttg aaattgccaa ggcacttaat
     4861 aaacaggtgc gcttgttaat tctcgatgaa ccgacagcct cattaactga gcaggaaacg
     4921 tcggttttac tggatattat tcgcgatcta caacagcacg gtatcgcctg tatttatatt
     4981 tcgcacaaac tcaacgaagt caaagcgatt tccgatacga tttgcgttat tcgcgacgga
     5041 cagcacattg gtacgcgtga tgctgccgga atgagtgaag acgatattat caccatgatg
     5101 gtcgagcgag agttaaccgc gatttaccct aatgaaccac ataccaccgg agatgaaata
     5161 ttacgtattg aacatctgac ggcatggcat ccggtcaatc gtcatattaa acgagttaat
     5221 gatgtctcgt tttccctgaa acgtggcgaa atactgggta ttgccggact cgttggtgcc
     5281 ggacgtaccg agaccattca gtgcctgttt ggcgtgtggc ccggacaatg ggaaggaaaa
     5341 atttatattg atggcaaaca ggtagatatt cgtaactgtc agcaagccat cacccagggg
     5401 attgcgatgg tacccgaaga cagaaagcgc gacggcatcg ttccggtaat ggcggttggt
     5461 aaaaatatta ccctcgccgc actcaataaa tttaccggtg gcattagcca gcttgatgac
     5521 gcggcagagc aaaaatgtat tctggaatca attcagcaac tcaaagttaa aacgtcgtcc
     5581 cccgaccttg ctattggacg tttgagcggc ggcaatcagc aaaaagcgat cctcgcccgc
     5641 tgtctgttac ttaacccgcg catcctcatt cttgatgaac ctaccagggg tattgatatt
     5701 ggcgcgaaat atgagatcta caaattaatt aaccaactcg tccagcaggg tattgccgtt
     5761 attgtcatct cttccgaatt acctgaagtg ctcggcctta gcgatcgtgt actggtgatg
     5821 catgaaggga aactaaaagc caacctgata aatcataacc tgactcagga gcaggtgatg
     5881 gaagccgcat tgaggagcga acatcatgtc gaaaagcaat ccgtctgaag tgaaattggc
     5941 cgtaccgaca tccggtggct tttctgggct gaaatcactg aatttgcagg tcttcgtgat
     6001 gattgcagct atcatcgcaa tcatgctgtt ctttacctgg accaccgatg gtgcctactt
     6061 aagcgcccgt aacgtctcca acctgttacg ccagaccgcg attaccggca tcctcgcggt
     6121 aggaatggtg ttcgtcataa tttctgctga aatcgacctt tccgtcggct caatgatggg
     6181 gctattaggt ggcgtcgcgg cgatttgtga cgtctggtta ggctggcctt tgccacttac
     6241 catcattgtg acgttggttc tgggactgct tctcggtgcc tggaacggat ggtgggtcgc
     6301 gtaccgcaaa gtcccttcat ttattgtcac cctcgcgggc atgttggcat ttcgcggcat
     6361 actcattggc atcaccaacg gcacgactgt atcccccacc agcgccgcga tgtcacaaat
     6421 tgggcaaagc tatctccccg ccagtaccgg cttcatcatt ggcgcgcttg gcttaatggc
     6481 ttttgttggt tggcaatggc gcggaagaat gcgccgtcag gctttgggtt tacagtctcc
     6541 ggcctctacc gcagtagtcg gtcgccaagc tttaaccgct atcatcgtat taggcgcaat
     6601 ctggctgttg aatgattacc gtggcgttcc cactcctgtt ctgctgctga cgttgctgtt
     6661 actcggcgga atgtttatgg caacgcggac ggcatttgga cgacgcattt atgccatcgg
     6721 cggcaatctg gaagcagcac gtctctccgg gattaacgtt gaacgcacca aacttgccgt
     6781 gttcgcgatt aacggattaa tggtagccat cgccggatta atccttagtt ctcgacttgg
     6841 cgctggttca ccttctgcgg gaaatatcgc cgaactggac gcaattgcag catgcgtgat
     6901 tggcggcacc agcctggctg gcggtgtggg aagcgttgcc ggagcagtaa tgggggcatt
     6961 tatcatggct tcactggata acggcatgag tatgatggat gtaccgacct tctggcagta
     7021 tatcgttaaa ggtgcgattc tgttgctggc agtatggatg gactccgcaa ccaaacgccg
     7081 ttcttgattt tgataaaaat tttctcaaag ccggttacgt attaccggtt ttgagttttt
     7141 gcatgattca gcaggaaaag aaccatgttt actaaacgtc accgcatcac attactgttc
     7201 aatgccaata aagcctatga ccggcaggta gtagaaggcg taggggaata tttacaggca
     7261 tcacaatcag aatgggatat tttcattgaa gaagatttcc gcgcccgcat tgataaaatc
     7321 aaggactggt taggagatgt cgtcattgcc gacttcgacg acaaacagat cgagcaagcg
     7381 ctggctgatg tcgacgtccc cattgttggg gttggcggct cgtatcacct tgcagaaagt
     7441 tacccacccg ttcattacat tgccaccgat aactatgcgc tggttgaaag cgcatttttg
     7501 catttaaaag agaaaggcgt taaccgcttt gctttttatg gtcttccgga atcaagcggc
     7561 aaacgttggg ccactgaacg cgaatatgca tttcgtcagc ttgtcgccga agaaaagtat
     7621 cgcggagtgg tttatcaggg gttagaaacc gcgccagaga actggcaaca cgcgcaaaat
     7681 cggctggcag actgactaca aacgctgcca ccgcaaaccg ggattattgc cgttactgac
     7741 gcccgagcgc ggcatattct gcaagtatgt gaacatctac atattcccgt accggaaaaa
     7801 ttatgcgtga ttggcatcga taacgaagaa ctgacccgct atctgtcgcg tgtcgccctt
     7861 tcttcggtcg ctcagggcgc gcggcaaatg ggctatcagg cggcaaaact gttgcatcga
     7921 ttattagata aagaagaaat gccgctacag cgaattttgg tcccaccagt tcgcgtcatt
     7981 gaacggcgct caacagatta tcgctcgctg accgatcccg ccgttattca ggccatgcat
     8041 tacattcgta atcacgcctg taaagggatt aaagtggatc aggtactgga tgcggtcggg
     8101 atctcgcgct ccaatcttga gaagcgtttt aaagaagagg tgggtgaaac catccatgca
     8161 atgattcatg ccgagaagct ggagaaagcg cgcagtctgc tgatttcaac caccttgtcg
     8221 atcaatgaga tatcgcaaat gtgcggttat ccatcgctgc aatatttcta ctctgttttt
     8281 aaaaaagcat atgacacaac gccaaaagag tatcgcgatg taaatagcga ggtcatgttg
     8341 tagcgatgat gagaattgtc ggcgtcacat cagggaactc agcacagcca cctgatgcga
     8401 gcaaaagcct tatccgaccg acaaatcaga taaggctcga ggaaatgaaa ttacatatgc
     8461 gccgcgatta accgttggtt atcctggtac attgcgaaca ggtagttgtt ataactcttc
     8521 cctttggtcg agtagccctt cagcttgtga atcatcgctg tggcagtcac ttcctgatcc
     8581 gctttacgca gctgtgcacg cgatttacgg aacgaagagt aagccgggtg cgtattcagg
     8641 ttagtgacat aggcgctcac cgattctttg acagaactaa actgtgagta ccctttcact
     8701 ttacccggcg cattggtaca acgtcctttc atgcatttca tgccgaacag attgttattg
     8761 ttgcgcgcca gcttcgacgt tccccaaccg ctttctgctg cagccatcgt cgccaccata
     8821 ctggtaggga taatgtctac gcgttcaagc aaggtattcc acgggatttt tcgcgtatta
     8881 ccagaccact tcaccttgta gcgtttggcg atgtctttca gacgcgcacg ctcagcaggt
     8941 gcccattggc cctgatactg ttttgaaatc agccagttac gttctgcagt aatggctgca
     9001 ttttggctgg taatgtaagg cattacggtc cggagaaacg cctttttcct tggtgttccg
     9061 gaagggtatt ttcgcaaatc aggaagtgaa ctactctttg cactattgcg agaatactct
     9121 tgtttactgc ttacctgttt attactagct ttagttaagt gggacttttg actcgctgtt
     9181 gttgtgtgcg tcttcgctaa cacctcactc gaaaacacca gagtgagtaa cataagaatc
     9241 atcgccccat atcgtcgtat gggagtcaaa atcatcaggt ctcctggtcg gatttaatca
     9301 ttccaacacc ttatattttt cacaaatttg agagttgaat ctcaaatcat atcaaaaata
     9361 gctgtcaaga gcaccccaag gaatagtcca aatctgaaac tatgtcacga gttaacgatt
     9421 cagattggcg ctaaatcgca gaaaatgtgg gggttatcgc aaaattcagc cgttttttgc
     9481 gcgcgatcgc tcacccttgc ttctcatcct gtggacttac cgctcaggga tgagttttgt
     9541 ctggcttatc gctggcaaac tgtctgaaat cgcagcaata aggactcatc caccatgaaa
     9601 ctcgccgcct gttttctgac actccttcct ggcttcgccg ttgccgccag ctggacttct
     9661 ccggggttcc ctgcctttag cgaacaggga acgggaacat ttgtcagcca cgcgcagttg
     9721 cccaaaggta cgcgtccact cacgctaaat tttgaccaac agtgctggca gcctgcagat
     9781 gcgataaaac tcaatcagat gctttccctg caaccttgta gcaacacgcc gcctcaatgg
     9841 cgattgttca gggacggcaa atatacgctg caaatagaca cccgctccgg tacgccaaca
     9901 ttgatgattt ccatccagaa cgccgccgaa ccggtagcaa acctggtccg tgaatgcccg
     9961 aaatgggatg gattaccgct cacgctggat gtcagcgcca ctttcccgga aggagccgcc
    10021 gtacgggatt attacagcca acaaattgcg atagtgaaga acggtcaaat aacgttacaa
    10081 cccgctgcta ccagcaacgg tttactcctg ctggaacggg cagaaactga cacatccgcc
    10141 cctttcgact ggcataacgc cacggtttac tttgtgctga cagatcgttt cgaaaacggc
    10201 gatcccagta atgaccagag ttacggacgt cataaagacg gtatggcgga aattggcact
    10261 tttcacggcg gcgatttacg cggcctgacc aacaaactgg attacctcca gcagttgggc
    10321 gttaatgctt tatggataag cgccccattt gagcaaattc acggctgggt cggcggtggt
    10381 acaaaaggcg atttcccgca ttatgcctac cacggttatt acacacagga ctggacgaat
    10441 cttgatgcca atatgggcaa cgaagccgat ctacggacgc tggttgatag cgcacatcag
    10501 cgcggtattc gtattctctt tgatgtcgtg atgaaccaca ccagctatgc cacgctggcg
    10561 gatatgcagg agtatcagtt tggcgcgtta tatctttctg gtgacgaagt gaaaaaaacg
    10621 ctgggtgaac gctggagcga ctggaaacct gccgccggac aaacctggca tagctttaac
    10681 gattacatta atttcagcga caaaacaggc tgggataaat ggtggggaaa aaactggatc
    10741 agaacggata tcggcgatta cgacaatcct ggattcgatg atctcactat gtcgctggcc
    10801 tttttgccgg atatcaaaac cgaatcaact accgcttctg gtctgccggt gttctataaa
    10861 aacaaaacgg atactcacgc taaagtcatc gaaggcttta cacctcgcga ttacttaacc
    10921 cactggttaa gtcagtgggt ccgcgactat gggattgatg gttttcgggt cgataccgcc
    10981 aaacatgttg agttgcccgc ctggcagcaa ctgaaaaccg aagccagctc cgcgcttcgc
    11041 gaatggaaaa aaactaaccc cgacaaagca ttagatgaca aacctttctg gatgaccggt
    11101 gaagcctggg gccacggcgt gatgcaaagt gactactatc gccacggctt cgatgcgatg
    11161 atcaatttcg attatcagga gcaggcggcg aaagcagtcg attgtctggc gcagatggat
    11221 acgacctggc agcaaatggc ggagaaattg cagggtttca acgtgttgag ctacctctcg
    11281 tcgcatgata cccgtctgtt ccgtgaaggg ggcgacaaag cagcagagtt attactatta
    11341 gcgccaggcg cggtacaaat cttttatggc gatgaatcct cgcgtccgtt cggtcctaca
    11401 ggttctgatc cgctgcaagg tacacgttcg gatatgaact ggcaggatgt tagcggtaaa
    11461 tctgccgcca acgtcgcgca ctggcagaaa atcagccagt tccgcgcccg ccatcccgca
    11521 attggcgcgg gcaaacaaac gacactttcg ctgaagcagg gctacggctt tgttcgtgag
    11581 catggcgacg ataaagtgct ggtcatctgg gctgggcaac agtgactttt ccggcttccc
    11641 gttcgtcagt acctcgggaa gccgccaacc aggataaaat gtcagcccta atcagcgttg
    11701 cgggataaag caccgctcac tcttcaacag accgatttgc accccagcaa atgtagcgtt
    11761 attgttacct tccttgctac agagttcgac agatatcccg ctatgacatt ctcccttttt
    11821 ggtgacaaat ttacccgcca ctccggcatt acgctgctga tggaagatct gaacgacggt
    11881 ttacgcacgc ctggcgcgat tatgctcggc ggcggtaatc cggcgcagat cccggaaatg
    11941 caggactact tccagacgct actgaccgac atgctggaaa gtggcaaagc gactgatgca
    12001 ctgtgtaact acgacggtcc acaggggaaa acggagctac tcacactgct tgccggaatg
    12061 ctgcgcgaga agttgggttg ggatatcgaa ccacagaata ttgcactaac aaacggcagc
    12121 cagagcgcgt ttttctactt atttaacctg tttgccggac gccgtgccga tggtcgggtc
    12181 aaaaaagtgc tgttcccgct ggcaccggaa tacattggct acgctgacgc cggactggaa
    12241 gaagatctgt ttgtctctgc gcgtccgaat attgaactgc tgccggaagg ccagtttaaa
    12301 taccacgtcg attttgagca tctgcatatt ggcgaagaaa ccgggatgat ttgcgtctcc
    12361 cggccgacga atccaacagg taatgtgatt actgacgaag agttgctgaa gcttgacgcg
    12421 ctggcgaatc aacacggcat tccgctggtg attgataacg cttatggcgt cccgttcccg
    12481 ggtatcatct tcagtgaagg gcgcccgcta tggaatccga atatcgtact gtgcatgagt
    12541 ctttccaagc tggggctgcc tggctcccgc tgcggcatta tcatcgccaa tgaaaaaatc
    12601 atcactgcca tcaccaatat gaacggcatt atcagcctgg cacctggcgg tattggtccg
    12661 gcgatgatgt gtgaaatgat taagcgtaac gatctgctgc gcctgtctga aacagtcatc
    12721 aaaccgtttt actaccagcg tgttcaggaa actatcgcca tcattcgccg ctatttaccg
    12781 gaaaatcgct gcctgattca taaaccggaa ggagccattt tcctctggct atggtttaag
    12841 gatttgccca ttacgaccga gcagctctat cagcgcctga aagcacgcgg cgtgctgatg
    12901 gtgccggggc acaacttctt cccgggactg gataaaccgt ggccgcatac gcatcaatgt
    12961 atgcgcatga actacgtacc agagccggag aaaattgagg cgggggtgaa gattctggcg
    13021 gaagagatag aaagagcctg ggctgaaagt cactaaaaat tgcctgatgc gctacgctta
    13081 tcaggcctac atgatttctg caatatattg aatttggaag aatttgtagg ccggataagg
    13141 cgtttacgcc gcatctggca taaacaaagc gcactattcc ggcattgaga aacgccggaa
    13201 aacgtttcaa acaggctgct gctgtagccg atgccgctgc actttctcga catccatgca
    13261 ctgcaacgcc tttgtcgggc aggcttcaac acaggccggc cccgtctccc gatgccagca
    13321 cagatcgcac ttaatcgccc tcgccttttt gcgcgaagaa acgacctcca tcgcaccaaa
    13381 cgggcaagcc agcatacagc ttttacagcc aatgcaacgt gtttgttcaa cgaaaatatg
    13441 cccatgttcg cggcttatcg cgtcaacagg gcagacattc gcgcacggtg catcttcaca
    13501 ctgatgacag gctactggcg tggtccagct gtggtcttta atgacacgaa tacgggaaat
    13561 aaactcgtct ggtgacaacg cagcgcaatc ctgattctcc tgatgcgaca ctgcgcaagc
    13621 cacttcacag gtacggcaac cgatacattt cgtcgcatcc gcaataataa accggttcat
    13681 gatcattcct ttgcactaaa acagcagagt gccagagagc gcgtccctac tgctttgatc
    13741 caacgaagga aatgttaaag ttttggcact tgtccaaagc gttatgtgat cgcgcccagg
    13801 tcatccctga cagtaaatcc cagttcatta gaaatagcct gcgcggtttc acgcagtggt
    13861 ttcaggagat ttttctctcc cacctgtttc agacgtgatg tcgaaagcga aatcgacacg
    13921 gcgtacggca cccgcccatg aatatcaaac accggaacag caatacagga gacgccgagt
    13981 tcgttttctt ctctgtccat cgccgctccg ctttcacgaa tgtgcgccag ttcgtcgaac
    14041 atcgcgggca gctcggtaat ggtattgcgg gttaacggct ggatctcatg ctgatggttt
    14101 tcccagtatg acttcacgta gtccgggtga ccaaacgcca tgtagatctt gcccattgcg
    14161 gaacagtaga gcggcatatg ctggccaata taggcacggg ttcgcagcat cccggttgtg
    14221 ggttccagct tataaatcaa aatagcgtga tcgtcttcgc ggctggagaa gttaatggtt
    14281 tcaccagtgg cgatgttcag tgcctcaaga tgcggagcgg cgatatgaat gatattcagc
    14341 gaagacagcg ccttctgccc gacggcaata aatttggtag tcaggcggta actccctgcg
    14401 gcgggcgcgg tggtcacata gccgcaggac tgtaatccct gcaataagcg atggacggta
    14461 ctcttattta aaccagccag ctccgaaaga tgcgccaacg gacaaccgtt tggatagttg
    14521 ctcaaaatct caatcagcat caacccgcga aacagactct ggcttccggc tggacgctct
    14581 ttttcctgcg ccatctcgtt ctcttttttt cccatcactt ctttccccat tttgtcgcgt
    14641 cctgatggta gcgcaaagtg tgccgtagtt cacgatctcg acagataatt tataaccact
    14701 tgatttttat gtcttttgaa attcatcaat cagattgctt tgttaaaaag tgatcgatat
    14761 atttgaaacc tagttccgca tattgaaatt ttaagccaaa aaagcgatca aaaaacaagg
    14821 aagcctagga tgaaagtgac atttgagcag ttaaaagcag cctttaatcg ggtcttaatt
    14881 tcacgcggcg ttgacagcga aacggctgac gcctgtgcag agatgttcgc ccgcaccacc
    14941 gaatccggcg tttattctca cggcgttaat cgtttccctc gtttcattca acaactggaa
    15001 aacggcgata tcattcctga tgcccaaccc aaacgtataa ccagcctcgg cgcaattgaa
    15061 cagtgggatg cccagcgttc gatcggcaac ctgacggcga aaaagatgat ggatcgcgcc
    15121 attgaactgg ctgccgatca cggtattggt ctggtggcac ttcgtaatgc caaccactgg
    15181 atgcgcggcg gcagctacgg ctggcaggcg gcggaaaaag gctatattgg catttgctgg
    15241 accaactcca tcgccgtaat gccgccgtgg ggcgcaaaag agtgtcgcat cggcactaac
    15301 ccgctgatcg tcgccattcc ttccacgccg atcaccatgg tcgatatgtc gatgtcgatg
    15361 ttctcttacg gcatgttaga agttaaccgc ctggcaggcc gtcagctccc ggtcgatggt
    15421 ggctttgatg atgagggcaa tttgaccaaa gaacctggcg ttatcgagaa gaatcgccgc
    15481 attttgccga tgggctactg gaaaggttct ggcatgtcga ttgtgctgga tatgatcgct
    15541 actctccttt ccgacggcgc atcggttgcc gaagtcaccc aggacaacag cgacgaatac
    15601 ggcatttcac aaatttttat tgccattgaa gtggacaagc ttatcgacgg tcccacccgc
    15661 gatgccaagc tgcaacgtat catggattac gttactaccg ctgaacgcgc tgacgaaaac
    15721 caggccatcc gcttacccgg ccacgaattt actaccctgc tggccgaaaa ccgccgtaac
    15781 ggcatcactg ttgatgacag cgtgtgggcc aaaatccagg cgttatgagg agataagtca
    15841 tgatttttgg acatatcgcg cagcccaatc cgtgccgttt gcccgccgcc attgaaaagg
    15901 cgctcgattt tctgcgcgct accaacttca acgtcctgga gccgggcgtt gtcgaaatcg
    15961 acggcaagaa tatttatgcg ca
//
LOCUS       DAZPEU010000080        15778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPEU010000080 DAZPEU010000000
VERSION     DAZPEU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.080"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            31..738
                     /locus_tag="ACHU2L_002279"
     CDS             31..738
                     /locus_tag="ACHU2L_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1357117.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            735..2186
                     /gene="djlC"
                     /locus_tag="ACHU2L_002280"
     CDS             735..2186
                     /gene="djlC"
                     /locus_tag="ACHU2L_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR1357118.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2246..3916)
                     /gene="hscC"
                     /locus_tag="ACHU2L_002281"
                     /pseudo
     CDS             complement(2246..3916)
                     /gene="hscC"
                     /locus_tag="ACHU2L_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(4000..4935)
                     /gene="rihA"
                     /locus_tag="ACHU2L_002282"
     CDS             complement(4000..4935)
                     /gene="rihA"
                     /locus_tag="ACHU2L_002282"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR1357119.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(5053..5778)
                     /gene="gltL"
                     /locus_tag="ACHU2L_002283"
     CDS             complement(5053..5778)
                     /gene="gltL"
                     /locus_tag="ACHU2L_002283"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR1357120.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5778..6452)
                     /gene="gltK"
                     /locus_tag="ACHU2L_002284"
     CDS             complement(5778..6452)
                     /gene="gltK"
                     /locus_tag="ACHU2L_002284"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR1357121.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6452..7192)
                     /gene="gltJ"
                     /locus_tag="ACHU2L_002285"
     CDS             complement(6452..7192)
                     /gene="gltJ"
                     /locus_tag="ACHU2L_002285"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR1357122.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7362..8270)
                     /gene="gltI"
                     /locus_tag="ACHU2L_002286"
     CDS             complement(7362..8270)
                     /gene="gltI"
                     /locus_tag="ACHU2L_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR1357123.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            complement(8667..10205)
                     /gene="lnt"
                     /locus_tag="ACHU2L_002287"
     CDS             complement(8667..10205)
                     /gene="lnt"
                     /locus_tag="ACHU2L_002287"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR1357124.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10230..11108)
                     /gene="corC"
                     /locus_tag="ACHU2L_002288"
     CDS             complement(10230..11108)
                     /gene="corC"
                     /locus_tag="ACHU2L_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR1357125.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11198..11665)
                     /gene="ybeY"
                     /locus_tag="ACHU2L_002289"
     CDS             complement(11198..11665)
                     /gene="ybeY"
                     /locus_tag="ACHU2L_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR1357126.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11662..12702)
                     /locus_tag="ACHU2L_002290"
     CDS             complement(11662..12702)
                     /locus_tag="ACHU2L_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR1357127.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(12855..14279)
                     /gene="miaB"
                     /locus_tag="ACHU2L_002291"
     CDS             complement(12855..14279)
                     /gene="miaB"
                     /locus_tag="ACHU2L_002291"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR1357128.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14425..15600
                     /gene="ubiF"
                     /locus_tag="ACHU2L_002292"
     CDS             14425..15600
                     /gene="ubiF"
                     /locus_tag="ACHU2L_002292"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR1357129.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         3840 a         4028 c         4093 g         3817 t
ORIGIN      
        1 cagttaatgc acttaaggga ttattaaaaa atgaataagg aagaacaata tcttcttttt
       61 gcgctttctg cgcccatgga aatacttaat cagagttgca aacccgcgca tgactcgcca
      121 aaaatgtata ccgggataaa agaattcgat ctctcatcat cctggggaat aaataatcgt
      181 gatgatttaa ttcagactat ttatcagatg actgatgatg gtcatgccaa tgatttagct
      241 ggcttatatc tgacatggca acgctcttcc cctgaagagt ggaaagcatt aattgctggt
      301 ggctcggaga gagggttgat ttatacccaa ttcgttgccc aaaccgctat gtgctgtgga
      361 gaagggggaa tcaaagcctg ggattatgtt cgaatgggat ttctgtcacg ggccggtgtg
      421 ctcaacaact ggctgacgga agaagagagt ttatggctgc aatcgcgcgt ctatgcacga
      481 gctcaccatt actatcacag ctggatgcac tatttttctg cctattcact agggcgtctc
      541 tactggcaat cttctcagtg cgaagacaac gcttcgctac gcgaggcgct aaccctgtac
      601 aaatacgata gcgccgggag tcggatgttc gaagagctgg cagcgggaag cgaccgtttc
      661 tatgccacgc ttccctggca gccattaact gtccagcctg aatgcccggt aacgcttaag
      721 gatgtgagcg acctatgaaa acatgttggc aaatcctcga aattgaaagc acgacgcaaa
      781 tagacattat ccgccaggct tatcttgctc gcttaccgtt gtgtcatccc gaaaccgatc
      841 cgcaggggtt taaagcatta cgccaggcct atgaagaggc gctgcgactg gcggtaaatc
      901 ctgtcgagga agcagatgat gaagaaaaag atgccaccgc tgaacatgaa atactacgtg
      961 cattcaggac attactggat tcagaaagtg atcgttttca gccctccgcc tggcagaaat
     1021 ttattcagca attaaatacc tggaacatgg aggatgtcga tcaattacgc tggccgctgt
     1081 gtgcaatcgc tatagaagcg cgatatctgt cattaaattg tgcttctttg ttggcagagc
     1141 gtttgaactg gcattcgttt aatgacagcg aaggaatgga tgaggaagaa agggaggctt
     1201 ttcttgaggc cattcaggct ggtgattgtt tcgatttcct tagccttctg gaatatcccg
     1261 tcgcgttaca gaaccagact gttgaatatt acttcgcgct ggaacgttgc tgccgttacc
     1321 atcctgacta tgtcactgcg tttttggcga tggaaggacc gtggttcatt cctgatgatg
     1381 caaagttaca tcgcaaactg ttgcgctggt acagctcggt gcaaacaggt atggtggaac
     1441 tcattcctgt agccaaacag tggcaagcgg aagaaccaga aagcgaagat gcccggtatt
     1501 accagtgtgc acaacgtttg tactgcggcg agggggaaag cctgcttgct gatctcggcg
     1561 cgtactggga aagttaccca tctacacaag ctgataattt gttgttgcag tggagcaagc
     1621 gtcattgccc ggattatttc gcgttattag tgatggttat cgaagcgcgg agcatggtag
     1681 atgcgcaagg tcaaccgctg aaatatgttc ctggtgagag cgcccggacg cggctgttat
     1741 gggcggaaat tttacatagc ggaaaattat cgccgttagg tcaatcgttt attgagtcgt
     1801 tattcttcaa acgcaaagca tgggcgtggt ggaaatcgag agtcggtagc gagacagaag
     1861 aagattcacc gttactggat ttgtatcggg tagcggaaca ggtagtactt gaagcgtttc
     1921 cgaaacaaga gatgctggcc cgtcttaata caaggctgga aggcggagat gctcatccat
     1981 tagaggccat tatcacccgg atgctgttga cgaaagtaaa actcgagccg gaggatgaag
     2041 atgtcgatga gccaacacct gaaaaccatg aagaaaaaaa tgatgagggt gaaaaaccac
     2101 agagcattac cagcattatc aaaatcagtt taacggtgct ggttataggt tatgctctcg
     2161 gcaaaatcgc gatgttgttt agttgacgat gtgttttttg cgccgatgcc ggatgcagca
     2221 tttacgccgc atccggcagc cgtgattatg aggtctcaat ggctaaataa acgctcgccc
     2281 gccgcctgac ctcatcgacg cgggccatat cgtttgactg cagcgccgca tcaaaatcgg
     2341 tgatcacccg gccaatctct tctcgctcgt cacccagcgc ccgcgcccac aactcttcca
     2401 gtttggcttt aaaggtgcga ttgatgagca tatcgcgcgg ataaattttc aatgcggata
     2461 accgcgtccg actctcttca atctgctgcg cgctcaatgt taccgggctg tggttaatca
     2521 ctctggactt aacgctgccg tcttccagaa gcacgtcaac ttcaagcaac ccgttgatat
     2581 cataactaaa gcgaatatca atcgactgat aagccccggt tttcttcaac ggcacatcga
     2641 aggattccac cagaatgttg tttttaactt tgtggttttc tccctgatag acgttaaccg
     2701 taattgaatc ctgttccggg tgcatggttg aataagtttc tacacgcgac acgggcacag
     2761 tggtgtttcg ttcaataatc ggcgagaaaa tgccggaaac gccctggcgg ttaacctcaa
     2821 cgcccaacga gtaagggcaa atatcagtga ggatcacttc ttcaatatct tcactgcgta
     2881 agcggcaggc ggcctggatt gctgcgccca gcgcgacaat ggtgctcgga tcgtaacttt
     2941 gatacggtaa tttgccaaac agacgcacgg cgattcgctg caccagcggc atttgtgacg
     3001 cgccgccaac cagcaccaga ctgtcgattt gactcggctt cagacgcgca tcgcgcaacg
     3061 cctgttcaat cggcactcgc aagcgattga gcagcggcaa ccacaaatct tccagttcgt
     3121 tctcgtaaaa ttcgcattcc cgcgtttcat cctggtacta ccagcgaatg tgcattggcg
     3181 attgattgct acattttgcc gcttccacac aggcgtacaa ggctgccagt tcactctcgt
     3241 taagcgtggt ccttgtgaca tccgcgcgct ttaaaacgtc atcgaccaac atatgggtaa
     3301 aatcttcgcc accgaggaag ttgtcgccag cggaggcgtg aacttcaatc accggcgtgg
     3361 cgtactcaag caccgtaacg tcaaacgtgc cgccaccgag atcaaacacc agcgaacggg
     3421 tattttgttg ggtatgcagg ccatacgcca tcgcagctgc tgtgggttca ttaattaagc
     3481 gtaccgcatt taacccggct aagtccgctg ctaaacgggt atgcttgcgt tgttcatcgc
     3541 tgaaataagc cagaacggag atcaccacat ctttaatcgg acgttgcaga aattcttcgg
     3601 catcttcttt taatgagcgt aataccaaag aggacagttc tggcgcgtta aatgtgtcgc
     3661 tgcctaaccg ccagttggta ttactgccca ttgcacgttt aaataacgct gccgttttat
     3721 ccggatgcga agtacgccgt gatacagccg gttttccgac taaaatatga ttattttcat
     3781 ccatgctaat tatggatggt gttaaatatt caccgaactt atttggaatt aattgcgcgg
     3841 caccgtcttt ccagacggca attaaactat tggtagtgcc gagatcgata cctatggcga
     3901 gttctgcatt atccattgca tttatatctt gaaagaaaaa gagataaata gattaagacc
     3961 acccaggtaa cgcgtggtga tcttaatcaa tgacgagtgt taagcgtaaa atttcagacg
     4021 atcagccagt aaatcaacaa agccctgacg atcaacatcg acaattacag tggcattcgg
     4081 tttattgcca gtcagataat aataatcaac aaccgtcata ccctgggtat atttcccctg
     4141 tgtttccacg ccaacccagc gctcaacagt ggtaaacaac tcgggtttca acagccaggc
     4201 gatggtgcat gggtcatgca gcggtgcgcc gacaaagccc catttttcgt ctttatgata
     4261 ttcgaggaag aaatccagca gttcggcaac aatggttgaa acagggttgc caatcgcgcg
     4321 gaaacgctcg gtgtcttcaa cgtggatttg cgctttatga gtaacatcca gaccggccat
     4381 caccaccggg atccctgact ggaagacaat ttctgccgct tccgggtcaa cgtaaatatt
     4441 aaattcagcc gcaggcgtcc agttaccaag ccccattgcg ccgcccataa tcacgatacg
     4501 ggcaattttg ctatgcagtt ccggatggct attgagcagc aaggcaacgt tagtttgcgg
     4561 tccggtagaa acaatcgtga caggttccgc actttcgcgc agcgttttcg ccatcagctc
     4621 caccgccgta cagttttgcg gtgcgaacgt cggttccggt aatgccgggc cgtcgagacc
     4681 gctttcgccg tgcacattgt ccgcgataat caactcacgc attaacggtt ttaccgcgcc
     4741 gcctgccacc ggaatatcgg tgcgattaag caaggtcagc atacgcagaa cattgcgtaa
     4801 ggttttttct ggtgtctggt ttccggcgga agacgtaatt gctttgacat caagctctgg
     4861 tgaggcgagt gcgagaacta ttgcgatagc gtcatcatga cctgggtcgc aatctaacag
     4921 aattggcagt gccattgttg ctccttgttg tgtgcttctt tgcgacaagg gtaacgccag
     4981 gatgtaacag atacgagggg cgaaacgata aagcgtgaga tggcgcgcaa ttgggtatgt
     5041 gcgccagagt gattaatgca ggattttcgc gaggaagtct tttgcgcggt ccgatttcgg
     5101 atcatcgaag aaagcgtctt tcggcgagtc ttcgacaatt ttaccctcgt ccataaagat
     5161 cacccgattc gccactttgc gggcaaagcc catttcgtgg gtcaccacca tcatggtcat
     5221 tccttcgttc gccagttcca ccatcacgtc cagcacttcg ttgatcatct ccggatccag
     5281 cgccgatgtc ggttcgtcga acagcatcgc aataggagcc atacacaatg cgcgagcgat
     5341 tgccacgcgc tgctgctgac cgccggaaag ctgcgccgga aatttattgg catgtgcgga
     5401 aagtcccacg cgctccagca atttcaatgc tttttcacga gcgggcgctt tatcgcgttt
     5461 aagcactttc acctgcgcca gggtcaggtt ttcgataatc gacagatgag ggaacagctc
     5521 gaaatgctgg aataccatcc cgacgcggga gcgcagcttt gccagatcgg ttttcttgtc
     5581 gttaaccacg ataccatcga cggtgatttc accttgctgc actggttcga ggccgttgac
     5641 ggttttaatc agcgttgatt tgccggaacc agacgggccg caaaccacca ccacttcgcc
     5701 ttttttcact tcggttgagc agtcggtcag cacctgaaag tgaccatacc attttgaaac
     5761 atttttcagg gtaatcatta tgctgtcctt cttttcaagt agctgaccaa caacgacgcg
     5821 ctaaggctaa taacgaaata aacaaatccg gcaaacagga tcatctcgac ctgcgtacca
     5881 tcacgctcac caatggttga ggcggtacgg aagaaatcgg ccagagataa cacatacacc
     5941 agcgaggtat cctggaacag tacgatgccc tgagtgagca gcagcggcac catcgcgcgg
     6001 aaagcctgcg gcagaataat cagtttcatc gactgccagt gagtcatccc caacgccagc
     6061 gcggcgctcg actgaccacg ggaaatactt tgaataccgg cacggataat ctctgaatag
     6121 taggccgctt caaacatcga aaacgccacc atcgccgaaa ttaaacgaat atcatttttt
     6181 ggcgataatc ccagcacatt ttgcagaaat cccggcacaa tcaagtaaaa ccacagcaaa
     6241 accatgacta aaggaatcga gcggaatacg ttaacgtagg ctttggcaaa ccacgaaaca
     6301 ggcgcgaagc tggacaagcg catcaccgcc agcatggtac cccacaaaat accaatcacg
     6361 actgccgtga cggtgatttt cagggtgatc accagcccgt cgagcagata tggcagggaa
     6421 ggaacaatgg aactccagtc aaactcgtac attatttgcc ccccatgttg ccgggcaggc
     6481 gaactttacg ttcaaccagc gtcatcacca gcatgataaa agcgttaatc aacacatacg
     6541 caagcgtgat ggcggtaaac gactcccagg cgtgagccga gtagtcgagc aatttacccg
     6601 cctgcgccgc catatccacc agaccgatgg tcgaggcgat ggcggagttt ttcaccaggt
     6661 tcatcatctc ggaagtcatc ggcgggacga taacgcgata agcattaggc agcagtacgt
     6721 atcgataagt ctgcggtagc gtcaggccca tcgccagcgc ggcattcttc tgcccacgcg
     6781 gtagcgactg aatagcggcg cgtacctgtt cgcaaacacg ggcggcggta aacagcccca
     6841 ggcagagcat ggatgaaagg aaaaactgaa tattgggatc cagctcggcc ttaaaccaca
     6901 tgccgatttt ctccggcagc agctccggga tcaccagata ccaggtaaag aattgcacaa
     6961 tcaacggcac gttgcggaac agttcgacat acaacgtacc cagaccggag agaaaacggt
     7021 ttggaacggt acgtaaaatg ccgaaaaatg aaccgacgag gaaagcgata atccaggcgc
     7081 agatcgacaa agcgatcgtc acctgaaaac cactccagac ccaaccaaga taggtggtat
     7141 tgccgaacgg ggcttgttgt aaaaaaatac cccagttcca gtctatagac ataaatctac
     7201 tccagaaaaa agagggtagc agcgttaact gctaccctcg aagattgtta cccagcgtat
     7261 tgcggttttc aggcccgatg gggaacgacc atcaggcgta tagtctgtcc gtgctacgta
     7321 acaatcgaga gggctggaat ttccgcccct ggttcttgta attagttcag tgccttgtca
     7381 ttcggttctt tgaacagtgc tttcatttcg tctgacagtt cgaaattcat gttcaggttt
     7441 ttcggcggaa ttggattttt gaaccattta tcaaaccatt tttccgcttc accggaggtc
     7501 tgcacctgag cgatagtgtc atccatcagc tttttgaact gcggatcgtc tttacgcaac
     7561 atgcaaccgt aggcttcctg agactgcggc ttgccgacga tatcccagtt gtctggtttc
     7621 ttcgctttcg cacgttcacc ggccagcaga gcgtcatcca tcataaaggc aacggcacga
     7681 ccgctttcca gggtgcggaa agagtcaccg tgatctttgg cgctgatgat gcgcatattc
     7741 attttttgct cttcattcag tttgttgagc aaaacttcag aggtagtgcc ggaagtgacg
     7801 actacggctt tgcctttcag gtcggcaaaa tctttgatat cgccgccctt tttggtcaac
     7861 aggcgcgtac cgaccacgaa aatagtgtca gagaaagccg cctgtttttg gcgttcgacg
     7921 ttgttggtgg tagaaccaca ttcaaaatcg aaagtgccgt tttgcagcag tggaatacga
     7981 ttttgtgagg taatcggaat cagttttacc tgcaagtccg gtttgttgag tttctttttc
     8041 actgcttcaa caatggcgtt ggagtaatcc tgcgagtaac ccaccacttt ttgctgattg
     8101 tcgtaataag agaaaggtac tgaagattca cggtgaccga cgacaatcac accgtttttg
     8161 gcgattttgt ccagcgtact gcctgctgcc ggggcggcgt catctgcctg tgccagtcct
     8221 gcggaaagcg ccagggcgag gattgctgtg gcaggtttac gtaattgcat atccaacacc
     8281 tttatcttct gcgttaagaa cgcatggata cccgttgtga gtgtttgtgt tgttatcgtc
     8341 tgcaacttta ttgtgcagtg ttgtgcctgt taggtaacat ttagtttggc taaatgtaaa
     8401 gatattgatg ttttattgtt tgtttttgcg aggtgcaccg caccattctg aagcaaaatt
     8461 cttaaaatgc actcttttgg tgctaccggt ggattgctgt ggtgcaaccg ggttgtactg
     8521 atgctggttc aagctttgcc ttgtataaca aagcaataga tgtgccaaag ttggatagga
     8581 gaatattgtt atccggataa tgcactgatg ccgcatccgg tgagcgtgcc gaaatatggg
     8641 atgtattccg gcacgataag aagggattat ttacgtcgct gacgcagact catcaacaca
     8701 gcagcaaaac caaacagtgc cgtcagcacc cacagcggcc agttgccggt acgtgcgtat
     8761 ggtgtgagtc cggtggtcgg cgtcacgtta gtggttaaca cctcgcgggc gaactgcggg
     8821 atcatcgcct gaatctcacc ctgcgggcca atcaccgccg taatgccgtt gttggtgctg
     8881 cgcaacagtg ggcgcgccag ctccagcgca cgcattcgcg ccatctggaa gtgttgccat
     8941 ggaccaatag atttaccaaa ccacgcatcg ttggagatag tcagcaggta gtcggtatcc
     9001 gggcggaagt tatcgcgcac ttgctcggcg agaataatct cgtagcaaat agccgcagta
     9061 agctcaatac catttgccga cagcggcggc tggatatatg gcccacggct gaacgacgac
     9121 atcggcagat caaagaacgg tgctaacgga cgcagaatcg actccagcgg gacaaactcg
     9181 ccaaacggca ccagatggtt tttgttatag cgatcggctg attcgtagct gtacggtgca
     9241 cctttaccca gcgtgatgat ggtgttgtag gtatcgtagc ggttctgctt attgagacgc
     9301 gcgtcgacaa tcccggttac cagcgagcta cctttatcac gcaactcacc gtccagtgct
     9361 ttgaggaacg gttgctgatt aatttccaga tcggttatcg ccgactccgg ccagataatc
     9421 aacgatgatt tgcccatcag cggtgccgtt gcgttgtagt aaatctttag cgtattaaga
     9481 agctggcctt cgtcccattt cagcgattgc ggaatatcgc cctgaaccat cgaaacctga
     9541 atggttttct ccggttgcgg ggtaaaccat tggatgtaac gcagcgggaa gggtagggca
     9601 aacagcacga cggccaccac cagcggacgc cagttgcgtt tgaccaacgc cagtgccagc
     9661 aggccactaa ccatcatcag caggaagtta atggcttcca cgcccattag cggtgccagc
     9721 ccttttaacg ggccatcaat ctggctatag ccgaactgta accacgggaa gccagtcagc
     9781 acccaaccgc gcagaaactc ggtcacttgc cagagagcag gggtggcaat cgccacgcgc
     9841 agccaggtgg ttttcggcca cagacgcgac agcacgccag caaacagtcc ggtatacagc
     9901 gacaaatacg ccgccagcag caccaccagg aagatgttaa ccgggccagg catcccgcca
     9961 aaggtcgcga tgctgacata gacccagtta ataccgctgc caaagaggcc aaatccccag
    10021 caaaagccaa tagcggcaga ctggagtggg cgacggttaa aggtcaacgc ctgaagcccc
    10081 atcagcgaaa taatcgccgc aggccagacg tcgtaaggag agaaggccag cgttccgcag
    10141 gcaccgaata ataacgccag cagcaggcga atgcgctggc gttcaattaa tgaggcaaaa
    10201 tccatgtagt tatctatcca gtttcggttt tattcatcca gctttggctg gggtgagtca
    10261 tccgggattt tgacatgaac ctgaataata cgccgactat cggccatcgc cactttgaac
    10321 tggtaaccgt cgatgtcgat agtttcgcca cgcgccggaa gatgcccaaa cgcctgcatc
    10381 accagaccac cgatagtgtc gacttcttca tcgctaaagt gggtgccgaa cgcttcgttg
    10441 aagtcttcaa tggaagccag tgcgcgcacg gtccaggtat gacgactcag ctgacggaag
    10501 tcgatatcat cttcttcgtc atactcgtct tcaatctcac caacaatcag ttccaggatg
    10561 tcttcaatgg tcaccagacc ggaaacccca ccgaattcgt caataacgat cgccatgtgg
    10621 taacgctgag agcgaaactc tttcagcatc cggtctacgc gcttactttc aggaacgaca
    10681 accgcctgac gtaacacttt gtccatgctg aaggcttcag catcgctgcg cataaacggc
    10741 agcaagtctt tcgccatcag aatcccttca atgtgatctt tgtcttcgct aatcaccggg
    10801 aaacgtgagt gggcggactc gatgatgaca tcaaggcatt cgtccagcgt ctggttgcgt
    10861 ttcagggtaa tcatctggga gcgggggatc atgatgtcgc gaacgcgttg gtctgcgatg
    10921 tccatcaccc cttcgagcat atcgcgcgta tcttcgtcga taaggtcgtt ctgcccggaa
    10981 tcacggatca gcgccagcag ttcgtcacgg tttttcggtt caccgtggaa aagttggctg
    11041 agtaacaggg agaaaaatcc cttcttgttg cttatcgtgt cactactgtg tgaattgtcg
    11101 tcgctcatgg cgtcgtatgg gttctcatgt tagttaatca aaacgccgtc gttaatcacc
    11161 aacggcgggg acgtctgcca gtcaaatgcc tggcaaatta ttctttctcg gcaatgtacg
    11221 gatcctcata gcccagagca agcataatct ctgtttcgag ggcttccatt tcttctgctt
    11281 cgtcatcttc aatgtgatcg taacctaaca aatgcagact gccgtgcacc accatatgcg
    11341 cccagtgcgc ctccagtggt ttgccttgct cctgagcttc cttctcaacc acctgacggc
    11401 agataaccag atcgcccagt agcgacatct ccatgccagg cggcacttca aacgggaagg
    11461 agagcacgtt ggtcggctta tccttaccgc gataggtcag attcagactg tggctttcgg
    11521 cggtatcgac cacgcgaata gtcacttccg actcttcctg aaactgcggg atcaccgcat
    11581 tcagccatgt ttgaaactgg ctctcttccg gtaacccgga attatcttca catgccagtt
    11641 gtaaatcgag gatcacctga ctcatttttg ttcctgttct tcgcgcttgc gttctgctgc
    11701 cagcgccgct ttacgttttt gttctgcttc ttcccaggct tcataagcgt taacgatacg
    11761 cgccaccacc gggtgacgaa ccacgtcttc gctgtggaag aagttaaagc tgatctcttc
    11821 gacatcggcc agcacttcaa tggcatgacg taagcctgat ttagtattac gcggcaggtc
    11881 gatctgtgtg acgtcgccgg tgataaccgc ttttgagtta aaaccgatac gggtcaggaa
    11941 catcttcatc tgttcgatgg tagtgttctg gctctcatcg agaatgataa acgcgtcgtt
    12001 cagcgtacga ccacgcatat aggccagcgg cgcgacttca ataacgttgc gctcaatcag
    12061 tttctcgact ttctcaaagc ccagcatttc aaacagcgcg tcgtacagtg ggcgcagata
    12121 cgggtctact ttctggctta aatcgccagg caggaagccc agtttctcac cggcttcgac
    12181 cgccggacga gtcagcagaa tacggcgaat ttcctgacgc tccagggcat caactgccgc
    12241 agccactgcc aggtaggttt tacccgtacc cgccgggcca acgccgaagg taatgtcatg
    12301 gtcgagaata ttggcgatgt actgcgcctg gtttggcgtg cgcggcttaa ttacgccgcg
    12361 tttagttttg atattgaccg ctttgccgta ctccggcacg ctctccgcgc tttgctccag
    12421 tacccgcgct tctttaatcg caaggtggat ctgttccggt tcgatatcct gaatctgacc
    12481 gcgcatcggg gcagtatcga catacaggct acgcagaatg tctgccgcag cggtgacgca
    12541 aatcggacgg ccggtcagtt taaagtggtt atcgcggcga ttgatctcga tgccgagacg
    12601 gcgttcgagc tgcttgatgt tgtcatcaaa cgggccgcac aggctcaaca gacgcgcatt
    12661 gtctgctggc tccagggtga tttcgcgagt gtctatgttc aaaccgttcc tcttatctgt
    12721 atgccgccgg aagctgaaca ttcaccggcc tataaggaaa ttattcacgc cacaggaaaa
    12781 aggcgcaagc attgcgataa agatggggat aaagagagaa aaaacaaggc ccaccggaac
    12841 ggcaggcctg agaattacgg ctgataataa cccacgccaa ggtcgttttc tttgcgggtg
    12901 cgggcaatca ccgactccgg tgtttctgcc atgcgcagac ccatttcatc ttcagttcgc
    12961 accactttac cgcgcagaga gttcgggtag acgtcggtaa tttctacatc gacgaattta
    13021 ccgatcatat ccggtgtgcc ctcgaagttg accacgcggt tattttcggt acgcccggaa
    13081 agctccatga tgctcttacg cgaagtccct tctaccagaa tacgctgggt ggtgccgagc
    13141 atccgacggc tccatgccat cgcttgctga ttgatacgtt cttgcagaat atacagacgc
    13201 tgcttcttct cttcttccgg aacatcatca accatatcgg cggcaggcgt tcctggacgt
    13261 gcagagaaga taaagctgta gctcatgtcg aaattgacgt cggcaatcag cttcatcgtt
    13321 ttctcgaagt cttcggtggt ttcgccaggg aagccaacaa tgaagtcaga actgatctga
    13381 atatccggac gcgccgcacg cagtttacgg atgatcgctt tgtactccag cgccgtatgg
    13441 gtacgtccca tcaggttcag aatgcgatcg gaaccgctct gtaccggcag atgcaggaag
    13501 ctcaccagct caggcgtgtc gcggtacact tcgatgatat cgtcggtgaa ttcgatcgga
    13561 tggctggtgg taaagcgaat acgatcgatc ccgtcgatcg cagcaaccag acgcaacaga
    13621 tcggcaaacg atccggtggt gccgtcgtag ttctcaccac gccaggcatt cacattctga
    13681 ccgagcaggt tgacttcacg cacgccctga gccgcaagct gggcaatctc aaacagaata
    13741 tcgtcagacg gacggcttac ctcttcacca cgggtgtaag gcaccacgca gtaggtgcaa
    13801 tatttattgc agccttccat gatggagaca aacgcggtcg gcccttcggc gcgcggttcc
    13861 ggcagacggt caaacttctc gatttccggg aagctgatat ctacaaccgg gctgcggtcg
    13921 ccgcgcacgg agttgatcat ctccggcaga cggtgcagcg tttgcggccc aaaaataata
    13981 tcgacatagt gggcgcgctg gcgaatgtgc tcgccttctt gcgatgccac gcagccaccg
    14041 acgccgataa tcaggtctgg attcttctct tttaacagtt tccagcgacc caactgatgg
    14101 aagacttttt cctgagcctt ctcgcggatt gagcaggtgt tcagcagtag cacatccgct
    14161 tcttccgcca cgtcggtcag ttgatagccg tgggtggcat ccagcagatc ggccatcttc
    14221 gatgaatcgt actcgttcat ctgacagccc caggttttaa tatggagttt tttggtcatc
    14281 gacttgctct tgcgaaatag tagccaggaa tgcagggcgc atagtgtaat gctttgctgc
    14341 cgttgtgacc agtatgagcg ttatcagccc ttgggggtaa aaatcctgta aacttaaagc
    14401 atattgctaa caggatgatt gaccatgaca aatcaaccaa cggaaattgc cattgtcggc
    14461 ggaggaatgg tcggcggcgc actggcgctg gggctggcac agcacggatt ttcggtaacg
    14521 gtgatcgagc acgcagaacc tgcaccgttt gtcgctgaca gccaaccgga cgtgcggatc
    14581 tcggcgatca gcgcggcttc ggtatcattg cttaaagggt taggggtctg ggatgcagta
    14641 caggctatgc gttgccatcc ttaccgcaga ctggaaacct gggagtggga aacggcacat
    14701 gtggtatttg acgcagccga attaaagttg ccgttgcttg gctacatggt ggaaaacaca
    14761 gtgctgcaac aggcgttgtg gcaggcgctg gaagcgcatc cgaaagtaac gttacgtgtg
    14821 ccaacctcgc tgattgcatt acatcgagat aatgatcttc aggagctgga actgaagggc
    14881 ggtgaaacga tccgcgcgaa actggtgatt ggtgccgacg gcgcaaattc tcaggtgcgg
    14941 cagatggcgg gaattggcgt tcatgcctgg cagtatgcgc agtcgtgcat gttgattagc
    15001 gtacagtgtg agaacgatcc cggcgacagc acctggcagc aatttacccc ggacggaccg
    15061 cgtgcgtttc tgccgttgtt tgataactgg gcatcgctgg tgtggtatga ctctccgacg
    15121 cgtattcgcc agttgcagaa tatgaatatg gcgcaactcc aggcggaaat cgcgaagcat
    15181 ttcccgtcgc gtctgggtta cgttacaccg cttgccgctg gtgcgtttcc gctgacgcgc
    15241 cgacatgcgt tgcagtatgt gcagccgggg cttgcgctgg tgggcgatgc cgcgcatacc
    15301 atccatccgc tggcggggca gggggtgaat cttggttatc gtgatgtcga tgccctgatt
    15361 gatgttctgg tgaatgcccg cagctacggt gaagcgtggg ccagttatcc tgtcctcaag
    15421 cgttaccaga tgcggcgcat ggcggataac ttcattatgc aaagtggtat ggatctgttt
    15481 tatgccggat tcagtaataa tctgccgccg ctgcgttttg tgcgtaatct cgggttaatg
    15541 gcggcagagc gtgctggcgt gttgaaacgt caggcgctga aatatgcgtt agggttgtag
    15601 ccttacaaca ttgccggatg cgtgccagcc gtaggtcgga taagacgcgg cagcgtcgca
    15661 tccgacattg aaggataaga cgtgtcaaca tcgcattcga cattgaatga acgcagaaaa
    15721 gcaaaaagct cgccgaagcg agctttttta atgtggctgg ggtacgagga ttcgaacc
//
LOCUS       DAZPEU010000081        15653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPEU010000081 DAZPEU010000000
VERSION     DAZPEU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.081"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            96..614
                     /locus_tag="ACHU2L_002293"
     CDS             96..614
                     /locus_tag="ACHU2L_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR1357130.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            604..1829
                     /gene="dgcN"
                     /locus_tag="ACHU2L_002294"
                     /pseudo
     CDS             604..1829
                     /gene="dgcN"
                     /locus_tag="ACHU2L_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            1845..2327
                     /locus_tag="ACHU2L_002295"
     CDS             1845..2327
                     /locus_tag="ACHU2L_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR1357131.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(2404..2751)
                     /gene="rplS"
                     /locus_tag="ACHU2L_002296"
     CDS             complement(2404..2751)
                     /gene="rplS"
                     /locus_tag="ACHU2L_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR1357132.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2793..3560)
                     /gene="trmD"
                     /locus_tag="ACHU2L_002297"
     CDS             complement(2793..3560)
                     /gene="trmD"
                     /locus_tag="ACHU2L_002297"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR1357133.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3591..4139)
                     /gene="rimM"
                     /locus_tag="ACHU2L_002298"
     CDS             complement(3591..4139)
                     /gene="rimM"
                     /locus_tag="ACHU2L_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR1357134.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4158..4406)
                     /gene="rpsP"
                     /locus_tag="ACHU2L_002299"
     CDS             complement(4158..4406)
                     /gene="rpsP"
                     /locus_tag="ACHU2L_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR1357135.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4543..5904)
                     /gene="ffh"
                     /locus_tag="ACHU2L_002300"
     CDS             complement(4543..5904)
                     /gene="ffh"
                     /locus_tag="ACHU2L_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR1357136.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6071..6862
                     /locus_tag="ACHU2L_002301"
     CDS             6071..6862
                     /locus_tag="ACHU2L_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR1357137.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6883..8169
                     /locus_tag="ACHU2L_002302"
     CDS             6883..8169
                     /locus_tag="ACHU2L_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR1357138.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8224..8817)
                     /gene="grpE"
                     /locus_tag="ACHU2L_002303"
     CDS             complement(8224..8817)
                     /gene="grpE"
                     /locus_tag="ACHU2L_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR1357139.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8940..9818
                     /gene="nadK"
                     /locus_tag="ACHU2L_002304"
     CDS             8940..9818
                     /gene="nadK"
                     /locus_tag="ACHU2L_002304"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR1357140.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9904..11565
                     /gene="recN"
                     /locus_tag="ACHU2L_002305"
     CDS             9904..11565
                     /gene="recN"
                     /locus_tag="ACHU2L_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR1357141.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11714..12055
                     /gene="bamE"
                     /locus_tag="ACHU2L_002306"
     CDS             11714..12055
                     /gene="bamE"
                     /locus_tag="ACHU2L_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR1357142.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12117..12407)
                     /locus_tag="ACHU2L_002307"
     CDS             complement(12117..12407)
                     /locus_tag="ACHU2L_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR1357143.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12397..12834)
                     /gene="ratA"
                     /locus_tag="ACHU2L_002308"
     CDS             complement(12397..12834)
                     /gene="ratA"
                     /locus_tag="ACHU2L_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR1357144.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13005..13487
                     /gene="smpB"
                     /locus_tag="ACHU2L_002309"
     CDS             13005..13487
                     /gene="smpB"
                     /locus_tag="ACHU2L_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR1357145.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13702..14064
                     /gene="ssrA"
                     /locus_tag="ACHU2L_002310"
     tmRNA           13702..14064
                     /gene="ssrA"
                     /locus_tag="ACHU2L_002310"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14269..15510
                     /locus_tag="ACHU2L_002311"
     CDS             14269..15510
                     /locus_tag="ACHU2L_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1357146.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         3964 a         4115 c         3718 g         3856 t
ORIGIN      
        1 tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg aaaaaatatc
       61 gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac cgactgttct
      121 tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct tctgacgttg
      181 cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg cctgcgctat
      241 cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc ctacaagaga
      301 atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa gaggcgatga
      361 tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag atggaattaa
      421 ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc gagtgcatta
      481 ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc gtaaacctgg
      541 atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc gcacggaaga
      601 aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag agcattacgc
      661 aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct gctttccgtg
      721 acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac agcagcaaca
      781 atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc aactgaaaca
      841 ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat aagcagcaaa
      901 atattctggc atcctggcat tacccccgta aggatccagg cgatactttc agcaatttca
      961 taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat ggtgaaacca
     1021 ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc tggttttcgc
     1081 tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc ctcacccgcc
     1141 atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta catgatgtgc
     1201 gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag tttcaccgct
     1261 tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt cgtttacagg
     1321 ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg ctggctaacc
     1381 gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc cgaaaaacgt
     1441 cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc tggggtcatg
     1501 cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt ggcgggctgc
     1561 gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat gatgtacagt
     1621 cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat ctcccgtttg
     1681 atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg atgaccattg
     1741 agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat caggccaaac
     1801 accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata aagcacctgg
     1861 tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct cagggaaagt
     1921 ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc gccggcgact
     1981 ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa ttgctcccgg
     2041 aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg ctaacacatg
     2101 cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa ggcttatcat
     2161 tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt ccacgcagca
     2221 atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag accgcccagg
     2281 gccgcgccga gaaccgccgc gtcgcagtgg tgattgcaac cccttaaaac acgagcaaac
     2341 agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg cttaagcgaa
     2401 atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac gcaggtagta
     2461 cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa ctaccggaga
     2521 gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa cagtgaatgc
     2581 agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct gcagacgttt
     2641 tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac ggaaggaagg
     2701 tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca taatttaatc
     2761 tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg tttatgttgc
     2821 tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc agtcagagcc
     2881 aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg tttcaaacgc
     2941 cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc catcccttct
     3001 aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa ggaatcttcc
     3061 gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga gtcaatcagc
     3121 gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca ttcttcgtca
     3181 atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac cagaatcaat
     3241 ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc ctgtggtgac
     3301 agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat ggcgtcccgc
     3361 aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc gtccacggta
     3421 cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag caggccattt
     3481 ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc aggaaacagg
     3541 ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt ttaaaaacca
     3601 ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat aacctgccca
     3661 tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt tgccttaatg
     3721 acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag atcgtagcct
     3781 tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc ctcttcaagc
     3841 tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt cgccgcatca
     3901 cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt ccagctttcc
     3961 agctggactt gctgccactg acccgccttc tggataaacc agggctgata gtcaaaaatg
     4021 ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc gtaagacgaa
     4081 cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg tttgctcatc
     4141 atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc agcaacgcga
     4201 tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc caggcgagtg
     4261 ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat gaagcgaccg
     4321 ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt tttagcgccg
     4381 tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac aaccggaccc
     4441 catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg atttctggag
     4501 aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac caggaaatcc
     4561 cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt tcttcatttt
     4621 cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt cctgtacctg
     4681 catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt ctggcttagc
     4741 gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca ctttatcgtc
     4801 catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc ccatcaagct
     4861 ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt tgagatcgaa
     4921 gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt caactttgct
     4981 ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg acgcgatgcg
     5041 gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga acttgatcgg
     5101 tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc cgtccacttt
     5161 ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg tattggccgc
     5221 atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa tcgacgcatg
     5281 gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag cggtatccac
     5341 cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa caatatcaac
     5401 cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg ccagcgtctc
     5461 aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca ctttcttctt
     5521 gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac cggcaccttg
     5581 caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg tctggttctc
     5641 ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct gccccggcgt
     5701 caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga taaactcacg
     5761 cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc gcagcgtatc
     5821 ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg tgcgcgacaa
     5881 acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt tggccgcaat
     5941 cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg ttgttatgca
     6001 acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg ctcaaactgt
     6061 cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg tcagtcttgc
     6121 gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg ctattatttc
     6181 tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc tgcccgacgg
     6241 tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt tggtgatctg
     6301 tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac ccattgtcta
     6361 tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat acatcaccca
     6421 tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt cctatgccac
     6481 gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc aactgaagaa
     6541 caagaagctg gcatttaacc aagaaatgcc gccattgatg agtatcgagc gtaaaatgtt
     6601 ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg gcctgttcta
     6661 catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta tcgtggcgtg
     6721 gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg gacgccgcgt
     6781 cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg gcagccgaat
     6841 tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca catttctact
     6901 actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt ttccgggtcc
     6961 gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa acagggtaat
     7021 cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat aagcctggtg
     7081 ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat tgtcgggatg
     7141 cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt tgtcgtactg
     7201 gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa agtcgcttat
     7261 ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt ctggttgctg
     7321 aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat cgtggttagc
     7381 ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc acaaatttcc
     7441 cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac cgttgatgac
     7501 atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg gaaatctatt
     7561 ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga ttcgctggac
     7621 gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga gaaaaaagag
     7681 ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc tgaaggtacg
     7741 ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg cctggtcgtc
     7801 aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga agagattgtt
     7861 ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc gcaaaacgac
     7921 ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc ctttaactgg
     7981 catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc actggaagag
     8041 atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat tctcgacgta
     8101 caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg cgagagtgtg
     8161 gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt acgaaaacag
     8221 aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac gaccattcag
     8281 cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat cagattccac
     8341 cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg cgatcacttc
     8401 aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa tgccttcaac
     8461 catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat ccaggctatc
     8521 aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg ctttttcaat
     8581 atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca aaataccgtc
     8641 acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt tttcatcgcg
     8701 cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt cgtgctgatc
     8761 catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac tactcatgaa
     8821 tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg gatcagtttc
     8881 agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac ctcggaaaaa
     8941 tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct actgcactga
     9001 caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc atcgttgagc
     9061 aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc gcggagattg
     9121 ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg ggcgcggcgc
     9181 gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac ctgggtttcc
     9241 tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg gaaggtcact
     9301 acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat tgccaaaaac
     9361 gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg catatgatag
     9421 agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat gggctaatta
     9481 tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct attctgaccc
     9541 cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca gcacgaccac
     9601 tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt aacgacctgg
     9661 aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat gtcctgattc
     9721 gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat ttcaatacat
     9781 taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc ctctttactg
     9841 tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata caggaaaacg
     9901 actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct tgagattgat
     9961 tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc tattgcaata
    10021 gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg taccggcgct
    10081 gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc cctgcgctgg
    10141 ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt gatcagcagt
    10201 gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca actgcgcgaa
    10261 ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact caccaaacct
    10321 gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact tcaggaaatg
    10381 accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca tcaacagtta
    10441 agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga acttaacgaa
    10501 tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg tctggcgaac
    10561 agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga cggtgaagac
    10621 gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt gattggcatg
    10681 gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca gattgctgaa
    10741 gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa ccgactgttt
    10801 gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca cgtcagccct
    10861 gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact ggacgatcag
    10921 gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca ggcactggaa
    10981 accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc acagctgatc
    11041 accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga tgtcaaatat
    11101 gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt aaccaccaac
    11161 ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt gtcccgcatc
    11221 gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact gatttttgat
    11281 gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact gctgcgtcaa
    11341 ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc gggatgtggt
    11401 catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac acatatgcaa
    11461 tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg cagtgaagtc
    11521 acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt tttcctgctt
    11581 cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt ttaaagtgat
    11641 gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg gcccgaaaag
    11701 gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac tattgatgtt
    11761 aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca accaggggaa
    11821 ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac aacaagttgc
    11881 gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct ggttctatgt
    11941 cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc tgacctttaa
    12001 cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta actaataata
    12061 aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc tgtctgttat
    12121 ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc aatgagaggg
    12181 cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc cggacggctg
    12241 taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag caagccactg
    12301 gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg caggtattgc
    12361 ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt aaacctcttt
    12421 cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca cgcgaccaaa
    12481 ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga tacgacacgc
    12541 ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg gcccatccac
    12601 cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa acgttttgct
    12661 gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg actccagaat
    12721 ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat tcactaactg
    12781 atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag gcataacaat
    12841 ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa aaacgctatc
    12901 ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg caggattgat
    12961 atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg aagaaaaaag
    13021 cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac gaatacttta
    13081 tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa tccctgcgcg
    13141 caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag gcatttctgt
    13201 ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc gatcctaccc
    13261 gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt cgcgtcaatc
    13321 gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg tgcaaagtga
    13381 aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc aaagaacgcg
    13441 aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc tgcactccaa
    13501 ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac atcttattgg
    13561 ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa atcaggctac
    13621 atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct cataaatctg
    13681 gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg cgaaacccaa
    13741 ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc gcaaacgacg
    13801 aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta gcctccgctc
    13861 ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc cctgcctggg
    13921 gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc cgcagctggc
    13981 aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt tcggacgcgg
    14041 gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag tcatccgatg
    14101 aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc gaatttgtcc
    14161 cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt taggcacttc
    14221 ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat ggcaagacaa
    14281 accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc ggactacgtt
    14341 ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa aatctggcag
    14401 tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg cccttacccg
    14461 tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact cctagctaaa
    14521 cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga agccaaaacc
    14581 aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag tgtgacagag
    14641 gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc gattggcgac
    14701 gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc ggttcaggcc
    14761 agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt catgatctat
    14821 gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa agccttcgaa
    14881 aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca actgatgcag
    14941 acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg gcaacttctt
    15001 actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga catagaagcg
    15061 caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac tgttccattg
    15121 tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa ccgagaattt
    15181 atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt taacgcctca
    15241 ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc aattgccagt
    15301 acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt ggcccatgtg
    15361 gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca gcgtcgcccg
    15421 atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat gattgaaggt
    15481 gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata gtgtcccact
    15541 ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc gcgttattag
    15601 gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa gtt
//
LOCUS       DAZPEU010000082        15526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPEU010000082 DAZPEU010000000
VERSION     DAZPEU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.082"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHU2L_002312"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHU2L_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR1357147.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHU2L_002313"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHU2L_002313"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR1357148.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHU2L_002314"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="ACHU2L_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHU2L_002315"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHU2L_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR1357149.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHU2L_002316"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHU2L_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR1357150.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHU2L_002317"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHU2L_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR1357151.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHU2L_002318"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHU2L_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR1357152.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHU2L_002319"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHU2L_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR1357153.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHU2L_002320"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHU2L_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR1357154.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHU2L_002321"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHU2L_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR1357155.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHU2L_002322"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHU2L_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR1357156.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHU2L_002323"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHU2L_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR1357157.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHU2L_002324"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHU2L_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR1357158.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHU2L_002325"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHU2L_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR1357159.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHU2L_002326"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHU2L_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR1357160.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHU2L_002327"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHU2L_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR1357161.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHU2L_002328"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHU2L_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR1357162.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHU2L_002329"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHU2L_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR1357163.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHU2L_002330"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHU2L_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR1357164.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHU2L_002331"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHU2L_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR1357165.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHU2L_002332"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHU2L_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR1357166.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHU2L_002333"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHU2L_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR1357167.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHU2L_002334"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHU2L_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR1357168.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHU2L_002335"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHU2L_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR1357169.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHU2L_002336"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHU2L_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR1357170.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHU2L_002337"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHU2L_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR1357171.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHU2L_002338"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHU2L_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR1357172.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHU2L_002339"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHU2L_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR1357173.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHU2L_002340"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHU2L_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR1357174.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHU2L_002341"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHU2L_002341"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR1357175.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHU2L_002342"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHU2L_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR1357176.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3642 c         4130 g         3747 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ttggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAZPEU010000083        15412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPEU010000083 DAZPEU010000000
VERSION     DAZPEU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.083"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1177)
                     /locus_tag="ACHU2L_002343"
     CDS             complement(<1..1177)
                     /locus_tag="ACHU2L_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1357177.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACHU2L_002344"
     tRNA            complement(1376..1460)
                     /locus_tag="ACHU2L_002344"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACHU2L_002345"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACHU2L_002345"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR1357178.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACHU2L_002346"
     CDS             2804..4306
                     /locus_tag="ACHU2L_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR1357179.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHU2L_002347"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHU2L_002347"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1357180.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHU2L_002348"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHU2L_002348"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1357181.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHU2L_002349"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHU2L_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR1357182.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACHU2L_002350"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACHU2L_002350"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR1357183.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACHU2L_002351"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACHU2L_002351"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR1357184.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACHU2L_002352"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACHU2L_002352"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR1357185.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="ACHU2L_002353"
     CDS             complement(11871..13067)
                     /locus_tag="ACHU2L_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR1357186.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="ACHU2L_002354"
     CDS             13260..13763
                     /locus_tag="ACHU2L_002354"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1357187.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHU2L_002355"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHU2L_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR1357188.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="ACHU2L_002356"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="ACHU2L_002356"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1357189.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3978 a         3933 c         3968 g         3533 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa tatctctata aa
//
LOCUS       DAZPEU010000084        15266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPEU010000084 DAZPEU010000000
VERSION     DAZPEU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.084"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(177..371)
                     /locus_tag="ACHU2L_002357"
     CDS             complement(177..371)
                     /locus_tag="ACHU2L_002357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357190.1"
                     /translation="MNGDRYEFTKDSTEDSVFHVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVET"
     gene            complement(461..1093)
                     /locus_tag="ACHU2L_002358"
     CDS             complement(461..1093)
                     /locus_tag="ACHU2L_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFR1357191.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            1197..1412
                     /locus_tag="ACHU2L_002359"
     CDS             1197..1412
                     /locus_tag="ACHU2L_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1357192.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            1529..1810
                     /locus_tag="ACHU2L_002360"
     CDS             1529..1810
                     /locus_tag="ACHU2L_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFR1357193.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            1845..2006
                     /locus_tag="ACHU2L_002361"
     CDS             1845..2006
                     /locus_tag="ACHU2L_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357194.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            1993..2823
                     /locus_tag="ACHU2L_002362"
     CDS             1993..2823
                     /locus_tag="ACHU2L_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR1357195.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            2820..4196
                     /locus_tag="ACHU2L_002363"
     CDS             2820..4196
                     /locus_tag="ACHU2L_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1357196.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIDELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            4269..4595
                     /locus_tag="ACHU2L_002364"
     CDS             4269..4595
                     /locus_tag="ACHU2L_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357197.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            4592..4792
                     /locus_tag="ACHU2L_002365"
     CDS             4592..4792
                     /locus_tag="ACHU2L_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357198.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            4804..4992
                     /locus_tag="ACHU2L_002366"
     CDS             4804..4992
                     /locus_tag="ACHU2L_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357199.1"
                     /translation="MDDSLEIFEEWCADELGVSAYFIRQMRSKNILGVIEYKRVEINK
                     RYRAWMAAVRAAGVKVKE"
     gene            4995..5159
                     /locus_tag="ACHU2L_002367"
     CDS             4995..5159
                     /locus_tag="ACHU2L_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357200.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            5140..5580
                     /locus_tag="ACHU2L_002368"
     CDS             5140..5580
                     /locus_tag="ACHU2L_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFR1357201.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGRSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKTE"
     gene            5577..6167
                     /locus_tag="ACHU2L_002369"
     CDS             5577..6167
                     /locus_tag="ACHU2L_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFR1357202.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            6164..6337
                     /gene="ninD"
                     /locus_tag="ACHU2L_002370"
     CDS             6164..6337
                     /gene="ninD"
                     /locus_tag="ACHU2L_002370"
                     /inference="COORDINATES: protein motif:HMM:NF036649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinD"
                     /protein_id="HFR1357203.1"
                     /translation="MKHCYRCGERKEDDRFRPGQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            6304..6479
                     /locus_tag="ACHU2L_002371"
                     /pseudo
     CDS             6304..6479
                     /locus_tag="ACHU2L_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            6482..6883
                     /locus_tag="ACHU2L_002372"
     CDS             6482..6883
                     /locus_tag="ACHU2L_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357204.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMMSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKANMTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            6843..7073
                     /locus_tag="ACHU2L_002373"
     CDS             6843..7073
                     /locus_tag="ACHU2L_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFR1357205.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDGQTSAKTM"
     gene            7045..7656
                     /locus_tag="ACHU2L_002374"
     CDS             7045..7656
                     /locus_tag="ACHU2L_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFR1357206.1"
                     /translation="MAKPARRRCKNEECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVVECKAIKAEYQQKLKDLRNSRSEAA"
     gene            7653..7859
                     /locus_tag="ACHU2L_002375"
     CDS             7653..7859
                     /locus_tag="ACHU2L_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFR1357207.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            7837..8502
                     /locus_tag="ACHU2L_002376"
     CDS             7837..8502
                     /locus_tag="ACHU2L_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR1357208.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NIDYDKEILAKALSHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            8499..9122
                     /locus_tag="ACHU2L_002377"
     CDS             8499..9122
                     /locus_tag="ACHU2L_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1357209.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            9555..9878
                     /locus_tag="ACHU2L_002378"
     CDS             9555..9878
                     /locus_tag="ACHU2L_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFR1357210.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            9862..10338
                     /locus_tag="ACHU2L_002379"
     CDS             9862..10338
                     /locus_tag="ACHU2L_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR1357211.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            10335..10772
                     /locus_tag="ACHU2L_002380"
     CDS             10335..10772
                     /locus_tag="ACHU2L_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1357212.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            10977..11519
                     /locus_tag="ACHU2L_002381"
     CDS             10977..11519
                     /locus_tag="ACHU2L_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1357213.1"
                     /translation="MNYPTVVNDIDFRDLIFVANNDPVTDSFMVAKAFGKLPKNVVRD
                     IERTIEACPPEFDTKLNFELCYKNNELQNGKPQKFYRLRKDGLMLLVMSDTKKEAMRI
                     KIAYINAFNWMYAMLQVGHRQFEEERNAVMLEYMKEKDVASMSGRLLNRWGKIKKPQL
                     MARIERLEQHGQTVIPGLIN"
     gene            11739..11954
                     /locus_tag="ACHU2L_002382"
     CDS             11739..11954
                     /locus_tag="ACHU2L_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1357214.1"
                     /translation="MDDNGNQWVNVEHRLPESKEGMWSKEVIALTDTGDVFKLSCMGS
                     YWQRTKAFIDSGASKVTHWMPLNYPDD"
     gene            12102..12344
                     /locus_tag="ACHU2L_002383"
     CDS             12102..12344
                     /locus_tag="ACHU2L_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFR1357215.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            12380..12868
                     /locus_tag="ACHU2L_002384"
     CDS             12380..12868
                     /locus_tag="ACHU2L_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR1357216.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            12846..14345
                     /locus_tag="ACHU2L_002385"
     CDS             12846..14345
                     /locus_tag="ACHU2L_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1357217.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
     gene            14346..>15266
                     /locus_tag="ACHU2L_002386"
     CDS             14346..>15266
                     /locus_tag="ACHU2L_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR1357218.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEW"
BASE COUNT         4467 a         3452 c         3904 g         3443 t
ORIGIN      
        1 actccattgt ttttaaatga aaaatagttt gtttttggtt atcgagattg aggtggggat
       61 tactggttgc aggttccgac tacatcacca acaaatgatt tggttgatgt aagttgttgc
      121 atacctggga tattcattac tttggagtaa agagcttttt tgtctgtagt gattgatcaa
      181 gtttcaacgg ttattcctcc tccagactgg tattctccta ccatagtgtt cgatgacaaa
      241 gcagtgtatt tcatctctgg atagacgcca gaaactgatt cataaactga tgatttatcg
      301 ccatttattg ttacgtggaa aacggaatct tccgtgctgt cttttgtaaa ctcgtaacga
      361 tcgccattca ttgacccgta cccgtgcagg tttgtgacaa tccagcattc agaattggcg
      421 ctggtagtta agagtattga gagtagcgcc gcaatcctga tcatacgaat tttaccctcg
      481 cttccacgac aacaccgata atcttgcagt tcccgttgat aggagtcata ggccatgaag
      541 ggttcaggcc tttcaggtac ttctgcccgc catctataac cagtttcttg aatgttgctt
      601 cgttcgcgtc agtcagtttg gctacaacaa ggcttccatt cactggctcg cgtccagtat
      661 ctactaacac catatgacct tcagggatgc tttgacctac aggtgaggtc atggaatcac
      721 cttcaacctt cagccagaat ccatcgccta ataagttaac gtcactgtca taccattcat
      781 caatgtcctt gatatcgtag ggttcacaag cttcacacca cgaaccagct ctaaccatgc
      841 taatcaatgg atatttccct ttgggctcaa catgcccaac aaatctaaca ttcgaatcag
      901 aggtgccatt gagcagccag tcaacactta cgccaagagc tgacgcaagt tctggtaaaa
      961 agcgtggtcg cttagtttta ccgttttcga gctgctctat agactgctgg gtagtcccca
     1021 ccttttgagc aagttcagcc tggttaagtc caagctgaat tcttttgctt tttaccctgg
     1081 aagaaatact cataagccac ctctgttatt tacctccaat cttcacaaga aaaactgtat
     1141 ttgacaaaca agatacattg tatgaaaata caagaaagtt tgttgatgga ggcgatatgc
     1201 aaactctttc tgaacgcctc aagaagaggc gaattgcgtt aaaaatgacg caaaccgaac
     1261 tggcaaccaa agccggtgtt aaacagcaat caattcaact gattgaagct ggagtaacca
     1321 agcgaccgcg cttcttgttt gagattgcta tggcgcttaa ctgtgatccg gtttggttac
     1381 agtacggaac taaacgcggt aaagccgctt aagacattcc cgctcttaca catcccagcc
     1441 ctgaaaaagg gcattaccag aaacaaatct ctatggtttt gcgtttcttt gcgaagccaa
     1501 ctctatctaa tcattaagga aattatctat gggtactatt gcaactaaaa gcaagaaagc
     1561 ggctcgcatc gagtcagcct tgctgaacaa actggcactg atggggcaga agacattcgc
     1621 tcgagcaatg ggggttcctg aatatcaggt aagccgatgg aagaatggtt tcttctcgca
     1681 ggtaagcatg atgcttgctg ttctggaata cggaatcgaa gacgatgaaa tggctgaatt
     1741 gactaagcgg cttgccgatt acctgacaaa agaaaaagcc ccgaagaacg gcgaattctt
     1801 cgaggcctaa tgtagaaaga ctggatcaat ccacgggagt aattatgaca aaacgtcgta
     1861 agaaatacca ggaaaaagaa gagattcgac accctgattc acctgaggga ttagtggtag
     1921 ccgcagcaaa taacagggcg ttcgcagagc gccttgttgg tgtttacaga ctagccaaag
     1981 caggagtgaa acatgggcgt cgttaagtta gctgattaca ggcctcaact ggaggtcgtg
     2041 gagcatcgcg tggcagaact cgaagatggc tacactcgga ctgcaaacac actgttagaa
     2101 gccgccatgc tttctggact tactctacat cagttactga ttgttatggc tgtgtggcgc
     2161 aagacatacg gttacaacaa aaaaatagat tggatcggaa acgaacagtt cgctgaactc
     2221 actggcatgg caccaaccaa atgctctacc gccaaaaacg agcttatcag aatgggagtt
     2281 ctcattcagg tggggcgtca agttggtatg aatacaaaca tttccgagtg gaaaacgaaa
     2341 gttaacggat tcggtaaaac atttaccaat tcggtaaaac aaaccttcac caaatcggta
     2401 aaaagcaatt taccgaatca gtcaaacaca aaagacaata tacaaaagac aataaataca
     2461 aataccccct taccccctaa cgggggcggc gatgggcagg ttaaacctga acgtcgcaag
     2521 gcagaacgaa tcgactacga atccttcctg aacgcctaca acaccgaagt cggtgacaga
     2581 ctgccacacg ctgttgcggt caacgagaaa cgcaaacgcc gcctgaagaa aatcatcccg
     2641 caactgaaaa cgccaaacgt ggacggtttc agagcgtatg tcagggcgtt tgtgcatcag
     2701 gccaagccgt tttacttcgg agacaacgac acgggctgga cggccgattt tgattacctg
     2761 ctgagggaag actcgttaac gggggtacgg gaagggaagt ttgcagacag ggggattgca
     2821 tgaaacagga tatcgaagcg agcgttatcg gtggcctgct gattggtgga ttaacaccaa
     2881 ccgccagcga cgttctggca acgctggagc cggaagcgtt ttcaattccg ctctaccgga
     2941 aagccttcga ggttatccgc aagcaggcgc gaaacagaaa cctaatcgac gcgctgatgg
     3001 ttgccgaggc gtgcggagag gagcatttca cgtcaatcct gatgaccagc aagaactgcc
     3061 cgagtgccgc aaacctgaag ggatatgctg gaatggtcgc ggataactat caccgccgtc
     3121 tggtgctgga aatcatggat gaaatgcgtg aaccaatcca aagcggaacc atcgacgcat
     3181 cgagtcaggc gatggatgaa cttgtaaagc gtctttcagc catcagaaag ccccgtgacg
     3241 aggtaaaacc tgtacggtta ggggaaatca tcactgacta cactgacacg cttgacaggc
     3301 gtctgaggaa cggagaagag tcagataccc tgaagaccgg aatcgacgaa cttgacgcca
     3361 tcaccggagg gatgaacgca gaagacctgg tgattatcgc cgctcgtcct ggtatgggta
     3421 aaaccgaact ggcgctgaag attgccgaag gcgttgcaag ccgcgttatt cctggttctg
     3481 acgtccggcg cggagtgttg attttctcga tggaaatgag cgcattgcag attgcagagc
     3541 ggagcattgc caacgccggg aggatgtcgg ttagcgtgct gcgaaatcct gcatcgatgg
     3601 atgacgaagg ctgggcgcgt gttgctaacg gcatgagtca gcttgcagat ttggatgtat
     3661 gggtagtcga tgcctcgcgg ttatcggtcg aagaaatacg ctcaatcgca gaacggcaca
     3721 aacaggaaaa tccaaacctg tcactcatca tggcggatta tcttggcctg attgagaagc
     3781 cgaaagcaga ccgcaacgac ctcgcaattg ctcacatctc cggaagcctg aaggcgatgg
     3841 cgaaagacct gaaaacgcca gttatctccc tgagtcagct ttcgcgcgat gttgagaagc
     3901 gaccaaacaa acgcccgaca aacgcagatt tgcgtgattc aggaagcatt gaacaggacg
     3961 cagactcaat catcatgctc tatcgggaag cggtatatga cgagaacagt agcgccgcgc
     4021 catttgctga aatcatcgtg acgaaaaacc gttttggctc gcttggtacg gtttaccagc
     4081 ggttctgtaa cggacacttt gttgcatgtg accaggatga agccagacag atttgcacag
     4141 catcaaatgc acctgctgcg cgtggcagac gatatgcaca aggggctgac gtatgaccat
     4201 ctacatcact gagataataa caggcctgct ggtaatcgca ggccttttta tttgggggag
     4261 agggaagtgt ggctgactgg caaattccaa tcatcattct tgccggagct tcgctggttg
     4321 ctggctttat cctgctgaaa aagcataaag accgtgatca aaaagttgaa gttctctatg
     4381 ggtatccagc gaacagcaca acatggctga ccatttacca ctaccgaaaa tcaggacgct
     4441 gggtattcga atgggatgat ctgttcgctg aaaagcgacc aaagtcatgg ggagacatca
     4501 gcgaatgcat gatgtttgaa gaaagaaaat ccggcgcaac ccgagaagag tttaacgaag
     4561 cgtgggcgcg attaagtgag agagggtatt tgtgagcaag tacgaaaaat tagatcaaaa
     4621 cattctttca atgctgagtg aaagaccaac acctgttttt gatatctggc ttaaatggcg
     4681 gagcaatgga atgtatatcg aaaccatcga tcgccgtatg caatacctga gaaagaaagg
     4741 gcttgttgca aatgtgcgtg ggaagggttg ggtgaaaatt aacctgtcat aacggggatt
     4801 gatatggacg atagcttaga aatatttgaa gagtggtgcg ctgatgaact tggtgtctcc
     4861 gcttacttca ttcgacaaat gcgaagcaag aatattttgg gcgtgattga gtataaacga
     4921 gttgaaataa acaagagata cagggcttgg atggccgcag ttcgcgccgc cggagtcaaa
     4981 gtgaaggagt gagtatgagc gcatacgaag aaatcatgtt agccctgcga ttctttttcg
     5041 gtgtggaaga agatgaaaac gtaaatgaga ttatcggaca agaccatgac ccgataggga
     5101 ctattgcagc tgcacttgac gattacagga gcgtaaatgg tgaggaaact aacgtttgaa
     5161 ctaagaagcc ccatccatca gcagaacgcc attcaagcta tccagcaaat ccttccagac
     5221 ccaaccaaac caatcgtagt aaccattcag gaacgcaacc gcagcttaga ccagaatcga
     5281 aagctttggg cttgccttgg tgacgtttcg cgtcaggttg aatggcatgg tcgctggctg
     5341 gatgcagaaa gctggaagtg tgtgtttacc gcagcattaa agcagcagga cgttgttcct
     5401 aaccttgccg ggaatggatt tgtggtaata ggccggtcaa ccagcaggat gcgtgtaggc
     5461 gaatttgcgg agctattaga gcttatacag gcattcggta cagaacgtgg cgttaaatgg
     5521 tcagacgaag cgcgactggc tctggagtgg aaagcgagat ggggagataa aactgaatga
     5581 acaaataccg acttatttac gcagatccgc cttggcaata tcgcgacaaa gccaacgatg
     5641 gcaatcgcgg tgctggacat aaatacgatg ttatgaatgt tcaggacatt tgccgactgc
     5701 cagtatggga tttagcggat ccagaatctt gcttgttagc gatgtggtgg gtgccgacac
     5761 agccagccga agcgctaaag gtaattgagg cgtggggatt caggttgatg actatgaaag
     5821 gctttacttg gcacaaaacc aataagcaca aaggcaacag tgcgatcgga atggggcata
     5881 tgaccagggc aaatagcgag gattgcttgt ttgccgttcg agggcggtta cctgagagaa
     5941 tggacgcttc aatatgccag cactttaccg caccgagaat ggagcacagt gcaaaaccac
     6001 cgatcgtaag agacatgtta gctaagttgc ttggcgacgt gccgcgctgt gagttattta
     6061 gccgcgacaa agtgaccggg tgggatatgt ggggaaatca gtgcgactcc gattttgaac
     6121 ttgctcccgg catggcgatt aaaccatgca aaatggtgat cgcatgaagc actgttatcg
     6181 atgtggagag cgaaaggaag acgatcgctt tcgaccagga cagccttact ggaatcgatg
     6241 gtgtctccgg tgtgaaagaa caccaacagg ggtgttacca ctaccgcagg aaaaggagga
     6301 cgtgtggcga gacagcgacg aagtatcacc gacataatct gtgaaactgc aaataccttc
     6361 caacgaaacg ctccagaaat aaacgcaagc caatcccaaa agaatctgac gtaaaaacct
     6421 tcaactacac ggctcacctg tgggatatcc ggtggctaag acatcgtgcg aggaaatgac
     6481 aatgctttta attcaacctg gatttggact tagcatcaaa aaaggccaca tgtttggcga
     6541 gaaagagtca caacgaaaaa tgatgtctat ccggttgcca tttatcagta tttattggct
     6601 aaacagggag gcaacaaatt attggtatac atgcgccaga gcagcattta acgaccctga
     6661 ctggtttgtg gaaaaccacc acgcagttcg tcaggcaaag agaaaggcca acatgacata
     6721 catgaaggcg tatcgaaaag catggaaaga acaccgcgat cgataccagc aagatatgga
     6781 aaagcttgaa tcagaaaaca tggaattaag acgaaagctt ggtgaagcaa aacgagacat
     6841 tgatgcttac aagcgacttt ttaatggtga aagccatgct tagtccatcc caatctcttc
     6901 aataccagaa agaaagcgtc gagcgggctt taacgtgcgc taactgcggt cagaagctgc
     6961 atgtgctgga agttcacgtg tgtgagcact gctgcgcaga actgatgagc gatccgaata
     7021 gttcaatgta cgaggaagaa gacgatggcc aaaccagcgc gaagacgatg taaaaacgaa
     7081 gaatgtcggg aatggtttca ccctgcattc gctaatcagt ggtggtgctc tccagagtgt
     7141 ggaaccaaga tagcactcga acgacgaagt aaagaacgcg aaaaagcgga aaaagcagca
     7201 gagaagaaac gacgacgaga ggagcagaaa cagaaagata aacttaagat tcgaaaactc
     7261 gccttaaagc cccgcagtta ctggattaaa caagcccaac aagccgtaaa cgccttcatc
     7321 agagaaagag accgcgactt accatgtatc tcgtgcggaa cgctcacgtc tgctcagtgg
     7381 gatgccggac attaccggac aactgctgcg gcacctcaac tccgatttga tgaacgcaat
     7441 attcacaagc aatgcgtggt gtgcaaccag cacaaaagcg gaaatctcgt tccgtatcgc
     7501 gtcgaactga ttaaccgcat cgggcaggaa gcagtagacg aaatcgaatc aaaccataac
     7561 cgccatcgct ggactgtcgt agagtgcaag gcgatcaagg cagagtacca acagaaactc
     7621 aaagacctgc gaaatagcag aagtgaggcc gcatgacgtt ctcagtaaaa accattccag
     7681 acatgctcgt tgaagcatac ggaaaccaga cagaagtagc acgcagactg aaatgtagtc
     7741 gcggtacggt cagaaaatac gttgatgata aagacgggaa aatgcacgcc atcgtcaacg
     7801 acgttctcat ggttcatcgc ggatggagtg aaagagatgc gctattacga aaaaattgat
     7861 ggcagcaaat accgaaatat ttgggtagtt ggcgatctgc acggatgcta cacgaacctg
     7921 atgaaaaaac tggagacgat aggattcgac accaaaaaag acctgcttat ctcggttggc
     7981 gatttggtcg atcgcggtac agagaacgtc gaatgcctgg aattaatcac attcccctgg
     8041 ttcagagctg tacgtggaaa ccatgagcaa atgatgattg atggcttatc agagcgtgga
     8101 aacgtcaatc actggctact taatggcggc ggctggttct ttaatatcga ttacgacaaa
     8161 gaaattctgg ctaaagctct ttcccataaa gcagatgaac ttccgttaat catcgaactg
     8221 gtgagcaaag ataaaaaata tgtcatctgc cacgccgatt atccttgtga cgaatacgag
     8281 tttggaaagc cagttgatca tcagcaggta atctggaacc gcgaacgaat cagcaactca
     8341 caagacggga tcgtgaaaga aatcaaaggc gcggacacgt tcatctttgg tcatacgcca
     8401 gcagtgaaac cactcaaatt tgccaaccag atgtatatcg ataccggcgc agtgttctgc
     8461 ggaaacctca cattgattca ggtacaggga gaaggcgcat gagactcgaa agcgtagcta
     8521 aatttcattc gccaaaaagc ccgatgatga gtgactcacc acgggccacg gcttctgact
     8581 ctctttccgg tactgatgtg atggctgcta tggggatggc gcaatcacaa gccggattcg
     8641 gaatggctgc attctgcggt aagcatgaac tcagccagaa cgacaaacaa aaggctatca
     8701 actatctgat gcaatttgca cacaaggtat cggggaaata ccgtggtgtg gcaaagctcg
     8761 aaggaaatac taaggcaaag gtactgcaag tgctcgcaac attcgcttat gcggattatt
     8821 gccgtagtgc cgcgacgcct ggcgcaagat gcagagattg ccacggtaca ggtcgtgcgg
     8881 ttgatatagc caaaacagag cagtggggga gagttgtcga gaaagagtgc ggaagatgca
     8941 aaggcgtcgg ctattcaagg atgccagcaa gcgcagcata tcgcgctgta acgatgctaa
     9001 tcccaaacct tacccaaccc acctggtcac gcactgttaa gccgctgtat gacgctctgg
     9061 tggtgcaatg ccacaaggaa gagtcaatcg cagacaacat tttgaatgcg gtcacacgtt
     9121 agcagcatga ttgccacgga tggcaacata ttaacggcat gatattgact tattgaataa
     9181 aattgggtaa atttgactca acgatggata aatgcactcg ttaaataaag ccctgagtta
     9241 atagctcggg gctttttgcg ttttaagcac ggcctttctg aaagcacatc aagccaaata
     9301 ccagacagac aaaaataatc accttatccg cttggctacg gtgcggtgtg ctttgcataa
     9361 aagaaaacca gcgcaatggc tggcttcgtg aaagcgggtg gcaagaggct gcgctaacaa
     9421 cctcatgccg ttttgcccgt gcatatcggt cacgaacaaa tctgattact aaacacagta
     9481 gcctggattt gttctatcag taatcgacct tattcctaat taaatagagc aaatcccctt
     9541 attgggggta agacatgaag atgccagaaa aacatgacct gttagccgcc attctcgcgg
     9601 caaaggaaca aggcatcggg gcaatccttg cgtttgcaat ggcgtacctt cgcggcagat
     9661 ataatggcgg tgcgtttaca aaaacagtaa tcgacgcaac gatgtgcgcc attatcgcct
     9721 ggttcattcg tgaccttctc gacttcgccg gactaagtag caatctcgct tatataacga
     9781 gcgtgttcat cggctacatc ggtactgact cgattggttc gcttatcaaa cgcttcgctg
     9841 ctaaaaaagc cggagtagaa gatggtggaa atcaataatc aacgtaaggc gttcctcgat
     9901 atgctggcgt ggtcagaggg aacagataac ggacgacaga aaaccagaaa tcatggttat
     9961 gacgtcattg taggcggaga gctatttact gattactccg atcaccctcg caaacttgtc
    10021 acgctaaacc caaaactcaa atcaacagca gccgggcgct atcagcttct ttcccgttgg
    10081 tgggatgcct accgtaagca gcttggcctg aaagacttct ctccgaaaag ccaggacgct
    10141 gtggcattgc aacagattaa agagcgtggc gctttgccga tgattgatcg cggtgatatc
    10201 cgtcaggcaa tcgaccgttg cagcaatatc tgggcttcgt tgcccggggc tggttatggc
    10261 cagttcgagc ataaggctga cagcctgatt gcaaaattca aagaagcagg cggaacggtc
    10321 agagagattg aggtatgagc agagtaaccg cgattatctc cgctctggtt atctgcatca
    10381 tcgtctgcct gtcatgggct gttaatcatt accgtgataa cgccatcgcc tacaaagagc
    10441 agcgcgacaa agccgcatcc actatcgctg atatgcagaa gcgtcaacgt gacgtagcag
    10501 aactcgacgc cagatataca aaggagcttg ctgatgctaa cgcgactatc gaaagcctcc
    10561 gtgctgatgt ttctgctggt cgtaagcgcc tgcaagtcgc cgccacctgt gcaaagtcaa
    10621 cgaccggagc cagcagcatg ggcgatggag aaagcccaag acttacagca gatgctgaac
    10681 tcaattatta ccgtctccga agcggaatcg acaggataac cgcgcaggtt aactacctgc
    10741 aggaatacat caggactcag tgcctgaaat aatttttttg caaatcacaa agtccattta
    10801 atgagcctcg cgatgcgggg cttttttaca tctgaatttc acagcgcatc tcacgcgcat
    10861 attacatcac ccgagccttt cagaaagttg agcctgagaa ctgccgtata tggtggcgac
    10921 catctcggga cggcttttct gtgagacagg ctcactttct aaaaggtaaa gacgcaatga
    10981 actacccaac cgttgttaac gatatagatt tcagagacct aatttttgta gcaaacaacg
    11041 atccggttac agattctttt atggtggcaa aagcatttgg aaagctgccg aagaacgtag
    11101 ttcgtgacat tgagcgaacc atagaagctt gccctcctga gtttgataca aagctcaact
    11161 ttgagctttg ctataaaaac aatgagttac agaatggtaa gccgcaaaaa ttctaccgtc
    11221 tccgcaagga tgggttgatg cttttggtta tgtccgacac caaaaaagaa gcaatgcgta
    11281 tcaaaattgc ttacatcaac gcatttaact ggatgtacgc catgcttcag gttggtcatc
    11341 gtcaatttga agaagagaga aatgccgtaa tgctggagta catgaaagag aaggatgttg
    11401 ccagcatgtc aggtcgcctg ctaaatcgct ggggcaaaat taagaagcca cagctgatgg
    11461 ctagaattga acgccttgaa cagcacgggc aaaccgtaat ccccggactc atcaattaac
    11521 ggcagcaccg cgaaacaacc caagccagta agtggggaaa taacactggc agccactgaa
    11581 agatgaacct ccagccttat ggcaaaaaag attctttgtg gtggcggact gatggaaaga
    11641 catcggatag aatcaagcag tggctagggt agctcccgaa aagcggactc gtcaccgcct
    11701 gccactgaat ctatggcgaa caactagacg aggttgtgat ggatgataac ggaaaccaat
    11761 gggtcaatgt tgaacaccgg ctaccagaaa gcaaagaagg gatgtggtct aaagaggtta
    11821 tagctcttac tgatactggt gacgtgttca aactatcatg tatgggctct tactggcaga
    11881 gaaccaaggc attcatcgat tcaggagcaa gcaaggttac acactggatg ccgcttaatt
    11941 acccagatga ttaaaacgga tgaaaaatga aaggtcgctc aggcggcctt ttttattgcc
    12001 atcacaaaag ccattcctta ctgagtggct ttgataatgg cttataccct acacgggata
    12061 acttaactga tatccctttt aacggataaa tggagccaac aatggcagag attattccca
    12121 tgactgaaga acagaaattc cagttagaga tttacaaact ggtcatgaac cagaacgcag
    12181 ccgcagaaga agcatttcaa ttcattggca ctgacgaact gaagcttgag ctattcaaaa
    12241 ttcacttcca gtcaggtggc gctaattcag atatcacgat ccgcacattt gaagcggtgc
    12301 gtaaatcgaa ggaagcgtta gacctgttca ccaccggagc atgatgctca acctgaaata
    12361 acgattaagt gagatgaata tggcagcacc aaagggcaac cgattctggg aggcccgcag
    12421 tagtcatggg cgaaacccta aattcgaatc gcctgaggcg ctgtgggctg cttgttgtga
    12481 atacttcgag tgggctgatg ataacccgct atgggagggt aaggtatttt catatcaggg
    12541 agaaataatt aaggctaatg tccctaagat gcgagccatg actatttcag gattgtgtac
    12601 cttccttgat atcaccaggc aaacatgggg aaccttccgg tcaatggaag gtttttctga
    12661 cgtcacatca cgagcggaag acatcatcta cgaccagaaa ttctctggcg cagccgctga
    12721 ccttctcaac gctaacatca tcgcccgtga tttgggcctc aaagagcagt cgcaagttga
    12781 agacgtgaca cctgataagg gagatcgcga taagcggcgc tctcgtatca aggagctatt
    12841 caaccgtgga actggacgcg attcttgata acttgagcga cgaagagcaa atcgaattgc
    12901 tcgagctact cgaagaagaa gagaactacc gtaacacaca cctgctatat gaatttacgc
    12961 catacagcaa acagcgtgag ttcatcgacg ccgggcatga ctatccagag cgctgtttta
    13021 tggctggtaa ccagcttggt aagtcattta ctggtgctgc tgaagtcgcg tttcacctta
    13081 ccgggcgtta tccgggcaca aaaggctatc ctgctgatgg taaatatggc ggtgagtgga
    13141 aaggtaagcg tttttatgag cctgttgtct tttggattgg tggcgagaca aacgagactg
    13201 taaccaaaac gactcaacgc atcctgtgtg gtcgtatcga agagaatggt gagccaggct
    13261 acggttccat acctaaagaa gacatcatta gctggaagaa gtctcctttc tttccgaacc
    13321 ttgttgatca ccttctggtt aagcatcaca cggctgatgg cgttgaagat ggcatttcaa
    13381 tctgctactt caagccatac tcgcaaggtc gtgctcgctg gcagggtgac acaatccacg
    13441 gcgtgtggtt tgacgaagag ccaccataca gcatttatgg tgaaggcctt acccgtacca
    13501 acaaatacgg gcaattctca attctgacgt ttaccccgct gatggggatg tctgacgttg
    13561 ttaccaagtt cctgaagaat ccaagcaagt cgcagaaagt ggtcaacatg accatctacg
    13621 acgctgagca ctacacagac gaacagaaag agcaaatcat cgcatcttat cctgagcatg
    13681 agagagaggc gcgtgctcgc ggtattccta cgatgggtag tggtcgaatc ttccagatac
    13741 cggaagagac gattaagtgt cagccgttcg agtgtcctga tcacttctac gtaattggcg
    13801 ggatggattt cggatgggat cacccgcagg cgcaggttca gctttggtgg gacaaggacg
    13861 cagacacaat atacgtttca cgcgtgtgga aggcgaaaga aaaaacagcc gttcaggcgt
    13921 ggggagccgt taaatcatgg gcgcataaag tgccaactgc atggcctcat gacggaaacc
    13981 agcacgagaa gggcggcggt gagcagctca aagggcagta tgcagatgct ggttttatga
    14041 tgttgcagga gcatgcgaca tggcctgatg gcggtaatgc tgtggagcct ggcatcactg
    14101 aattgcgcga catgatgctc gatggtcgct tcaaagtatt caacacctgt gagccattct
    14161 ttgaggagtt ccgcctctat caccgtgatg aaaacgggaa aatcgtcaag cttaacgacg
    14221 acgttctctc agccgttcgc tatgcataca tgatgcgccg cttcgccaaa atgatgcgcg
    14281 acatcaaaaa accaaaagag aaaaagatac cagccccaat caggcccatc gcacggagaa
    14341 cttaaatggc cgacgaaaac agactcaatt ccattctgtg taagtttgac gcggactgga
    14401 tggcgagcga tgaagccaga accgaggcga caaatgacct gtattttagc cgagtgtcgc
    14461 aatgggatga ctggctatca aactacacta ccctgcaata tcgcggacaa ttcgatgttg
    14521 ttcgcccgga ggtcaggaaa ctggtcgcag agatgcgccg gaaccctgtc gacgttctct
    14581 tccgacccaa agacggcgct aatcctgatg cagccgatgt attgatgggg atgtatcgta
    14641 ctgatatgcg ccataacacg gcaaagattg ccgttaacgt tggcgttcgt gagcagatag
    14701 agtcaggcgt tggtgcatgg cgtctggtca cgcagtacga agacaacgat ccaacaagca
    14761 acaatcaggt aatccgacgc ctgccaatcc atgaagcctg ctcacacgtc atatgggacg
    14821 ccaacagcaa gcagatggat aagagcgacg ctaagcactg cacggtgatt aacgccttgt
    14881 cgcgcaatgg ctggaaagag ttcgcagagg attacggtat tgacccggat accctgccat
    14941 ctttccagaa tcctaacgat acatggctat tcccgtgggt gtcgaatgat gtcgtctacg
    15001 tcgctgagta ttacgaggta gaagaggaga aggagaaggt cttcatctac cgcgacccgc
    15061 tgacaggtga gccggtcagc tattaccagc aggatatcaa agacgtcatc gacgacctgg
    15121 ctaatcgtgg attcattaag gtagcagagc gcaaggtgaa gcgtcggcgt gtgtataagt
    15181 cgatcatcac ctgtacgcag atactgaaag accgcgagaa gatagccgga gagcatattc
    15241 caatcgttcc agtgtatggc gaatgg
//
LOCUS       DAZPEU010000085        15198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPEU010000085 DAZPEU010000000
VERSION     DAZPEU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.085"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(126..2405)
                     /gene="maeB"
                     /locus_tag="ACHU2L_002387"
     CDS             complement(126..2405)
                     /gene="maeB"
                     /locus_tag="ACHU2L_002387"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR1357219.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDSLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2694..3644
                     /gene="tal"
                     /locus_tag="ACHU2L_002388"
     CDS             2694..3644
                     /gene="tal"
                     /locus_tag="ACHU2L_002388"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1357220.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3664..5667
                     /gene="tkt"
                     /locus_tag="ACHU2L_002389"
     CDS             3664..5667
                     /gene="tkt"
                     /locus_tag="ACHU2L_002389"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1357221.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5770..6813)
                     /locus_tag="ACHU2L_002390"
     CDS             complement(5770..6813)
                     /locus_tag="ACHU2L_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR1357222.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(6939..7514)
                     /gene="nudK"
                     /locus_tag="ACHU2L_002391"
     CDS             complement(6939..7514)
                     /gene="nudK"
                     /locus_tag="ACHU2L_002391"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR1357223.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7582..9561)
                     /gene="aegA"
                     /locus_tag="ACHU2L_002392"
     CDS             complement(7582..9561)
                     /gene="aegA"
                     /locus_tag="ACHU2L_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR1357224.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9767..11484
                     /gene="narQ"
                     /locus_tag="ACHU2L_002393"
                     /pseudo
     CDS             9767..11484
                     /gene="narQ"
                     /locus_tag="ACHU2L_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11686..14799
                     /gene="acrD"
                     /locus_tag="ACHU2L_002394"
     CDS             11686..14799
                     /gene="acrD"
                     /locus_tag="ACHU2L_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR1357225.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14898..14957)
                     /gene="ypfM"
                     /locus_tag="ACHU2L_002395"
     CDS             complement(14898..14957)
                     /gene="ypfM"
                     /locus_tag="ACHU2L_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR1357226.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3584 a         4018 c         3948 g         3648 t
ORIGIN      
        1 atatcgagat attttatata ctgatttttt tattattcgc aaactcagta ataagctttg
       61 ctaatatatt tcaggaaaac cgcttaccct gaatattcag ggtaagcgtg agagttaaga
      121 atgaattaca gcggttgggt ttgcgcttca accacggcca gcgccaccat attgacgata
      181 cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt tcgccacgcc catcagcacc
      241 ggaccgacag tcacaccttc cgagctggaa acgcgcagta agttgtaact aatgcgggca
      301 gcttccatgt tcggcatcac cagaatattg gcagaacctt tcaaagggct gtccggcata
      361 cggtcgttgc gaatcgcttc caccagcgct gcatcgccgt gcatttcacc atcaatcatc
      421 agatctggcg cacgatccct gaccagttcc agcgcctgac gcattttgct cgacgacggg
      481 cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa cgcgcggctc aataccaaaa
      541 cgacggacag tttctgccgc catcaaggtg atctccgcca gctcttctgc gtctggttcg
      601 tcattgacgt aggtatcggc aataaaggtg ttaccactcg gcagcagcag cgcattcatt
      661 gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat ttttcaccac actaaaatgt
      721 tcatgataat cgcccaccgt accgcagatc attgcatcgg cttccccacg ctgaaccatg
      781 atcgcgccga tcactgtcgg gttactgatc agcgcacgct gcgcctgttc ctgagtgaca
      841 ccgcgacgct tcatgatctg gaagtattcg gtccagtact ctttaaagcg cggatcggat
      901 tcgttattga cgatttcaaa atcaacgccc gctttgatct gcaagcccag tttctgaatg
      961 cgcatttcga tcacgttcgg acgaccgata aggatcggtt tcgccagtcc cagcgttacc
     1021 agttcctgag tcgcatgcag aacgcgcgcc tcttcccctt ccggcagaac tacgcgcttc
     1081 ggcgctttgc gagcctggga gaaaatcggc ttcataaaca ggttggtttt gtaaacgaac
     1141 tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg gacgcgtcgc cacgcccgac
     1201 tccatcgccg cttttgcgac cgcaggagcg atcttaacga tcaagcgcgg atcaaacggt
     1261 tttggaatga tgtattccgg accaaagctc agatcctgaa cgccatacgc tgaagccacc
     1321 acttcactct gttctgcatg ggccagttct gcaatcgcgc gtaccgccgc cagtttcatc
     1381 tcttcgttga tggcggttgc gccaacgtcc agcgcgccac ggaagatgaa cgggaagcaa
     1441 agaacgttgt tcacctggtt cgggtagtcg gaacggccgg tacagatgat ggcatccgga
     1501 cgcacttctt tcgccagcgg cggcagaatt tccggttccg ggttcgccag cgccaggatc
     1561 attggtgccc gagccatttt cttcaccatt tcctgggtca gcactttcgg gccggaacag
     1621 cccaggaaaa tatccgcacc ttcaatcaca tcatcgaggg tacgtttgcc gtcatccacc
     1681 accgcatatg cggctttggt ttccgccata tttggctcac ggccctgata gataacgcct
     1741 tttgaatcgc aaaccacgat gttatgtttt tgcagcccca gcgctaccag caggttcata
     1801 caggcgattg ctgcggcacc cgcgccggaa accaccatcc gcacgtcgga gatgtttttc
     1861 tccaccacgc gcaagccgtt gaggatggcg gcagtgctga tgattgccgt gccgtgctga
     1921 tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt tctgttcaat atagaaacat
     1981 tctggcgctt taatgtcttc gaggttgatg ccgccgaagg tcggttcgag cgcggcgacg
     2041 acttcaataa atttgtccgg gtcgagttca tcaacttcaa tgtcaaatac atcaatccca
     2101 gcgaatttct taaatagaac gcccttgcct tccatcaccg gtttgcctgc cagcgcgcca
     2161 atgttgccta accccagcac cgccgtaccg ttagagatca ccgcaaccag gttaccgcgc
     2221 gcggtatatt tgtaggcttt tagcgagtct ttttcgattt caagacaagg tgcggcaacg
     2281 cctggtgagt aggccagcgc cagatcgcgc tgcgttgcca gaggcttggt tggagaaacc
     2341 tggattttcc ctggaactgg aaattcatgg aaatcaagtg cactttgttt taactggtca
     2401 tccatttggt tgttcctttc acgtaacgtt cacaaataaa gtgtgtgggc aacagcccct
     2461 gcccacaacg tggcgcacat tattaccctg ccggagtcta cagactttga gcaagtccaa
     2521 actctcacca ttaatataat gttttggtaa taatcctata acactgatgt tacctgctta
     2581 atccagcaat accatgcctg tctgctatgc ttttttgatg cgtttagcga aatttctcag
     2641 aagtgtgaat taacgcactc atctaacact ttacttttca aggagtattt cctatgaacg
     2701 agttagacgg catcaaacag ttcaccactg tcgtggcaga cagctgcgat attgagtcca
     2761 ttcgccatta tcatccccag gatgccacca ctaatccttc gctgttactc aaggctgccg
     2821 gattatcaca atatgagcat ttaatagacg atgctatcgc ctggggtaaa aaaaatggca
     2881 agacccagga acaacaggtg gtcgcagcgt gtgacaaact ggcggtcaat ttcggtgctg
     2941 aaattctcaa aatcgtaccc ggttgcgtgt caacagaagt tgatgcacgc ctctcttttg
     3001 ataaagaaaa gagtattgag aaggcgcgcc atctggtgga cttgtatcag caacaaggcg
     3061 ttgagaaatc acgcattttg atcaaactgg cttcgacctg ggaaggaatt cgcgcggccg
     3121 aagagctgga aaaagaaggt attaactgca acctgacgct gctgttttct tttacccagg
     3181 cgcgggcctg tgcggaagca ggcgtttttc tgatttcgcc gtttgtaggg cgtatttatg
     3241 actggtatca ggcacgcaag ccgatggacc cgtatgtggt ggaagaagat ccgggcgtta
     3301 aatcggtgcg caatatctac gactactata agcaacacca ctatgaaacc attgtgatgg
     3361 gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac cggctgcgat cgactgacta
     3421 tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc tccagtggta cgtaaattaa
     3481 tcccgccttc tcagacgttc ccacgcccag cacccatgag cgaagcggag ttccgttggg
     3541 agcacaatca ggatgcgatg gcggtagaaa aactgtctga aggcattcgt ctgttcgctg
     3601 ttgatcaacg caaactggaa gatcttcttg ccgccaaact ataaaccagc cacggagtgt
     3661 tatatgtccc gaaaagacct tgccaatgcg attcgcgcac tcagtatgga tgctgtacaa
     3721 aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg ctgatattgc cgaagtgctg
     3781 tggaacgatt ttcttaaaca taatcctacc gacccaacct ggtatgatcg cgaccgcttt
     3841 attctttcca acggtcacgc gtcgatgctg ctctacagtt tgctacatct gaccggttac
     3901 gacctgccgc tggaagaact gaagaacttc cgtcagttgc attcgaaaac cccaggccac
     3961 ccggagattg gctatacgcc aggcgttgaa accaccaccg gcccgcttgg acaaggtttg
     4021 gcgaacgccg tcgggctggc gatagcagag cgtacactgg cggcgcagtt taaccagcca
     4081 gaccatgaga tcgtcgatca cttcacctat gtgtttatgg gcgacggctg cctgatggaa
     4141 ggtatttccc acgaagtctg ttcgctggca ggcacgctgg gactaggcaa gctgattggt
     4201 ttttacgatc acaacggtat ttccatcgac ggtgaaacag aaggctggtt taccgacgat
     4261 acggcaaaac gttttgaagc ctatcactgg catgtgatcc atgaaatcga cggtcacgat
     4321 ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg tgaaagataa gccgtcgctg
     4381 attatctgcc gtacggtgat tggctttggt tcgccgaata aagcaggtaa ggaagaggcg
     4441 cacggcgcac cactggggga agaagaagtg gcgctggcac ggcaaaaact gggctggcac
     4501 catccgccat ttgagatccc taaagagatt tatcacgcct gggatgcccg tgaaaaaggc
     4561 gaaaaagcgc agcagagctg gaatgagaag tttgccgcct ataaaaaggc tcatccgcaa
     4621 ctggcagaag agtttccccg acggatgagc ggtggtttac cgaaggactg ggagaaaacg
     4681 actcagaaat atatcaatga gttacaggca aatccggcga aaatcgctac ccgtaaggct
     4741 tcgcaaaata cgcttaacgc ttacgggccg atgctgcctg agttgctcgg cggttcggcg
     4801 gatctggctc ccagcaacct gaccatctgg aaaggttctg tttctctgaa ggaagatccg
     4861 gcgggcaact acattcacta cggggtgcgt gaatttggca tgaccgctat cgccaacggc
     4921 atcgcgcacc acggcggctt tgtgccgtat accgcgacgt tcctgatgtt tgttgaatac
     4981 gcccgtaacg ccgcgcggat ggcggcactg atgaaagcgc ggcagattat ggtttatacc
     5041 cacgactcaa ttggcctggg cgaagatggt ccgacgcacc aggctgttga gcaactggcc
     5101 agcctgcgct taacgccaaa tttcagcacc tggcgaccgt gcgatcagat ggaagcggcg
     5161 gtgggctgga agctggcggt tgagcgccac aacggaccga cggcactgat cctctcaagg
     5221 cagaatctgg cccaggtgga acgtacgccg gatcaggtta aagagattgc tcgtggcggt
     5281 tatgtgctga aagacagcgg cggtaagcca gatattattc tgattgccac cggttcagag
     5341 atggaaatta ccctgcaagc ggcagagaaa ttagcaggag aaggtcgcaa tgtacgcgta
     5401 gtttccctgc cctcgaccga tattttcgac gcccaggatg aggaatatcg ggagtcggtg
     5461 ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag gtattgccga ttactggtac
     5521 aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg gttacgggga atctgctccg
     5581 gcggataagc tgttcccgtt ctttggcttt accgccgaga atattgtggc aaaagcgcat
     5641 aaggtgctgg gagtgaaagg tgcctgatgg tgatcgccgg atgctgtatg ttcccctcac
     5701 cctaaccctc tccccagaag ggcgagggga cggtccgtgc gctttttagg gccgcatctg
     5761 cgattttgtt taacgggtga tccacaacgt gggcctggcg tctggcccat gccagttatc
     5821 gcaggtaggt tctgcggcgt aacgcaccag gcgaaaacgc tgaccgtcaa agcgccagcg
     5881 cgcctgaatg ccacaatcgc ttaatccgcg ccctttcgct aaggtcacca gttcacgcga
     5941 tttctcatca aatgttgcgt tcatcagttc cagttcattc gtctcctggc cgttgttgaa
     6001 cggcaaacgc aaccgaaccg ggcgcgaggc tagcggcttt ttacgcgaca caatccatgc
     6061 caaatcaatg gtgttatagg cccctgcctc acagctaatc atcatcagtg ctttatcatc
     6121 ggtcagcgca gtgacattca cctcacgacg taacggatca agcgagcagc gcagaccatt
     6181 catccgccag tttccataat ccagcaaatc gttacgttct tcgagtgaga gtggcgtcgg
     6241 cgttgggtta accaccgcga cctctttcag cgcaggcgcg ggcggtacgc tgagaggcgg
     6301 ttcgtcccct ttcttgatcc acgcggtttc actgccaacg cgcttttgct gagcatcaat
     6361 aaacaacaac gccgctttta agccgctcag agaaatggtc tgattgccgt cgcgtaaggt
     6421 gatcgccttc ccttcctgaa ttatctgcaa aaacgcggtg atggttgccg tatcatcggt
     6481 tactaataac catggtgaaa tccgccactt gtcaccactt aatgccaacg gctcaccatc
     6541 taatagcagc cgtggcgcta tctccccttc agacgcatcc ggcgacttca acccaccgcg
     6601 ctcaatacgt aaaacggcat cggtatgcgc cccagcgctg cggctcaggg tcatcaccag
     6661 tccattatga tcgcccgtat tgcgcgccac acaaaaattt tgattattac aggtgacctg
     6721 ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc aaagacgtcg gcaacaaagc
     6781 gaaaaaaaag agaaggaaaa tgcgatagcg catggacggt acgaccccag aagttcaaac
     6841 aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg ctcaatcgga tttgtcggat
     6901 tgagccaata ataaacaacg aaatattatt gctatttttc agtccattaa atgtgacatt
     6961 tgcaaatagt taagcaataa caccgtctta ccgtcacgta tctcgccggt tttgatcatc
     7021 tccagcgcct ggctgaacgg cagctcgagc acttcaatat cttcatcttc aacaccgcca
     7081 ccggcgttag cgcgctgatt gtcactgtat tcggcgataa aaaagtggat tagctcagtc
     7141 acaccgccag gcgacatata cagttcaaat aatttgcgca cttcgcccac ttcatagccc
     7201 gtctcttcaa tcgcttcttt acgaatgcac acttccggtt cgtcgttatc cagcagcccg
     7261 gcgcaggttt caatcagctg cccgctttca ttgccattaa cccaggtagc gacacggaac
     7321 tgacgaatca gaaccacgct ctttttcttc gcgttgtaca ggaggatcgt cgcaccatta
     7381 ccgcgatcgt aaacttcacg tttatggcgg ataacttcgc catctttgcg cgtgagatcg
     7441 taagtaatgt tgtgcagggt gaaatagtta tcggagagaa ttttgtcttt aatgagggtg
     7501 atttgttgcg tcataccgac tccacagcgc gaaatgaaca attatattac gctgtgaagt
     7561 cgggtttgtc tgcgcaggct atcagtgaga tttgactgat tttaccccca gccagtcaat
     7621 aatcccctgt gccgcatgac gtccttctgc cattgcggta accaccagat ccgcaccacg
     7681 cacggcgtcg ccaccggcga agattttcgg attggtggtc tggtaacggt actggctttc
     7741 cacatccgcg atgatgcggc cccatttgtc taccgttaca ccgtgcgact ccagccacgg
     7801 catcccgtgt ggattgaagc caaacgccat aatcaccgca tcggctggca tgacaaattc
     7861 actgccctca atcggtacag gtcgccgacg cccctgggca tccggctcgc caagacgcgt
     7921 gcgcaggaaa cgaattccgc aaacgtgacc ttgctcattc agctcaagcg ccaccggctg
     7981 gacgttaaat tcgaagttgg ccccctcttc gcgggcgttc ttcacctctt tcttcgagcc
     8041 tggcatgtta gcttcatcac gacgataagc gcaggtgacg ttactcgcgc cgtggcgcag
     8101 cgcagtacgc acacagtcca tcgcggtgtc gccgccgccc agtaccacga cgttaagtcc
     8161 ggcggtattg ataaacggct cttccggtag ctcttcgagc cccatcacct gtttagtgtt
     8221 ggcaatgagg aacggcagcg cgtcataaac gcccggcgca tcttcattgg gtaaacccgc
     8281 tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg tcgtattgtt ccaaaagcaa
     8341 atccaaagag acatctttac ccacttcaca attgagttcg aagtgaatcc ccatcgcgct
     8401 gaagatttcc cgacggcgtg ccagcaggga tttatccagt ttgaaagaag gaatgccgaa
     8461 agtgagcaag ccaccgattt ctggatggcg atcgtacacc gtcaccgcca cgccattgcg
     8521 ggtcagaaca tccgcacagg ccagccctgc cggacctgcc ccgataatcg ccacccgctt
     8581 gtccactttg gtgacatgac ttaagtcagg acgccaacct ttcgccaacg cctgatctga
     8641 aatatagcgt tcaatgttgc cgatagttac cgcgccgtgc tcatcgcgaa tagtacaggc
     8701 accttcacac aaacggtctt gcggacaaac gcgtccggta atttccggca gggtgttggt
     8761 ctggtgagaa agctcgacgg cggcgtcgat gtttccggct ttcaccagtt caatccactg
     8821 cggaatatgg ttatgcagcg ggcaggtcca ttcacaaacg ctgtgctcgc cgaacttaag
     8881 gcagcgcgag gcttcccgtt gtgcctggtc ggcgcgaaat ggcagataaa tttcatcaaa
     8941 accggttttg cgcgcttcaa tcgccagttt atccggctcg ccacgcgcgg gcgttgcctg
     9001 catttgttcg actttactca ttaccggcat ttcttgtgca gcggtactgg catgccacgg
     9061 ttgatgctcc tgacgtgcgg tgcgcaagcg gcgggatttc gccatgccgg agagtgcgac
     9121 gtcagtgacc agttgcagcg cgtccgccgg gcaattctcg acacaggcag gaccgttttc
     9181 gcgccctgca caaaggtcgc atttatgcgc cgtggctttt acttttcctg ccgcgacggg
     9241 tgtcaggacg atttgcatcg taccaaacgg acaggccacc acgcaggatt tacagccaat
     9301 acacttttgc tgattgacct gaatgctgtc atcaacgtgg ctgattgcgc cattagggca
     9361 gctacgggcg cagggcgcat cttcacaatg gtgacaggtc actgcactac gttgctgctg
     9421 atgtttgata accgtaattc ggggatgaaa atggtgttgg ctcaggacat gttgctcatc
     9481 attgtgagcc atgacacagg cgatttcaca agcatgacaa cccagacatt gctgactgtt
     9541 ggccataata aaacgattca taacgacctt cttttttggt tgtaaaaacc ttattcttta
     9601 tatgagtgtt gttattaccc gacttacagg ggaaccggca atgttcatgt gcccagaata
     9661 agtaactatt tcgctataaa ctgtggcaga tcaaataatc ccatcactga ctaaattgcg
     9721 tttcagcgaa ctggaacatt aatgattttt tgtggagaag acgcgtgtga ttgttaaacg
     9781 acccgtctcg gccagtctgg cccgggcctt tttttacatt gtgctgctgt cgattctttc
     9841 cacgggtatc gctctgctaa ctctggcgag cagtttgcgc gacgctgagg ctatcaatat
     9901 tgccggatcg ctgcgtatgc agagttaccg cctgggctac gacttgcaaa gtggcagtcc
     9961 acaactcaat gcacatcgcc agttgtttca gcaggcactg cattcaccgg tattaaccaa
    10021 cctcaacgtc tggtatgtgc cagaagcagt aaaaactcgc tatgcgcatc tgaatgccaa
    10081 ctggctggag atgaataatc ggctcagcaa gggcgatttg ccgtggtatc aggccaatat
    10141 taataattat gttaatcaga tagacctgtt cgtgctggct ttacagcact acgctgaacg
    10201 caaaatgctg ctggtggtgg cgatttccct ggctggcggt atcggtattt tcacgctggt
    10261 cttttttact ttgcgccgca tacgccatca ggtggttgcc ccgctgaatc agctggttac
    10321 cgccagtcag cgtattgaac acgggcagtt cgactcgccg ccgctggata ccagtctgcc
    10381 gaatgagctt ggtctgcttg caaaaacctt taaccaaatg tcgagcgagc tgcataaatt
    10441 gtacctttcg ctggaggcgt cagtagaaga aaagacccgc gatctccacg agaccaagcg
    10501 tcgtctggag gtgttgtatc agtgcagtgt tcccaggcgc tgaacaccag ccagattgat
    10561 gtgcattgtt tccgccatat tttgcagatt gttcgcgaca atgaagcagc tgaatatctg
    10621 gagctgaacg ttggggataa ctggcggatt agcgaaggga agccaaaccc tgagctgcca
    10681 atgcagattt taccggtgac aatgcaagag acggtttacg gcgaactgca ctggcaaaat
    10741 agtcacgttt catcatcaga accgctgctt aacagtgttt catcgatgct gggacgcggt
    10801 ttgtacttta atcaggcgca gaagcatttt cagcagttat tgttgatgga agaacgtgcg
    10861 accatcgccc gcgaattgca cgactcgctg gctcaggtgc tttcttactt acgtatccag
    10921 ttgacgttac tgaagcgttc gataccggaa gataacgcca ccgcacaaag tatcatggcc
    10981 gatttttccc aggcgttgaa tgatgcttat cggcagttac gcgagctgtt gactactttc
    11041 cgcctgacgc tgcagcaggc ggatctcccc tccgcgttga gggaaatgct ggatacgtta
    11101 caaaatcaaa ccagcgctaa actgaccctc gactgccgtc tgccaaccct ggcactggat
    11161 gcgcaaatgc aggtgcattt gttgcaaatt attcgcgaag cggtgctgaa tgcgatgaag
    11221 cacgccaacg ccagcgaaat cgccgtcagt tgcgtcaccg cgccggacgg caatcacacg
    11281 gtttatatcc gtgataacgg gattggtatc ggtgaaccga aagaacccga aggtcattat
    11341 ggactgaata tcatgcgcga acgcgcggaa cggctgggcg ggacgctgac tttttcacaa
    11401 ccttccggcg gcggcacgtt agtgagtatt agctttcgct ccgcagaggg tgaggggaag
    11461 gggaacacgc cggctgaggt gtaaataatt accctcacac cggccctctc cctttaaagg
    11521 cgagagggaa aaacgtgccc atattgacct gtaagcgagt gtgtcagcaa tctcatgata
    11581 atttacatta actccttttt ttctccacga ttggctcgta ccttgccgct acagtgaagc
    11641 aagtcaagcc tacaacaata cgcagaaaca cgaggtcctc ttttaatggc gaatttcttt
    11701 attgatcgcc ccatttttgc ctgggtgctg acaatcctgt tgtgtctgac aggtaccctg
    11761 gcgatttttt cattgcccgt tgaacaatac cccgatctcg cgccaccgaa tgtgcgagtg
    11821 accgctaact atcccggcgc atcggcccag acgctggaaa acaccgtgac ccaggttatc
    11881 gagcaaaata tgaccggcct cgataatctg atgtatatgt catctcagag cagtggcacc
    11941 ggtcaggcat ctgtcacttt aagttttaaa gcaggcaccg atccggacga agccgtgcag
    12001 caagtacaaa accagctgca atcagccatg cgaaagttac cgcaggcggt gcaaaatcag
    12061 ggcgtgacgg tgcgtaaaac cggcgatacc aacattctga ccattgcctt cgtctctacc
    12121 gatggctcga tggataaaca ggatattgct gattatgttg ccagtaatat tcaggacccg
    12181 ttaagccgcg tgaatggcgt cggggatatc gatgcctatg gttcgcaata ttccatgcgt
    12241 atctggctgg acccggcgaa actcaacagt ttccagatga cggctaaaga tgtcactgat
    12301 gccattgagt cacagaacgc gcagattgcg gttgggcaac ttggtggtac accttccgtc
    12361 gataagcagg cgctcaacgc caccattaac gcccagtcac tgctgcaaac accagaacag
    12421 ttccgcgata tcaccttgcg ggtgaatcag gacggctcag aggtaaggct gggcgatgtc
    12481 gccaccgtcg aaatgggggc ggagaaatac gattatctta gccgcttcaa tggtaagcca
    12541 gcctccgggt tgggggtaaa actggcctcc agcgcaaacg aaatggcgac agcggagctg
    12601 gtgctcaatc gtctcgacga gctggcgcag tatttcccgc acggactgga atacaaggtg
    12661 gcgtatgaaa ccacctcgtt tgttaaagcc tccattgaag acgtggtgaa aacgctgctg
    12721 gaagctatcg ctctggtttt cctcgttatg tatctgttcc tgcaaaactt ccgcgccacg
    12781 ctgataccca ctatcgccgt gccggtggtg ttgatgggaa ccttctccgt actttacgcc
    12841 ttcggttaca gcgtcaacac cttaaccatg ttcgcgatgg tgctggcgat cggtctgctg
    12901 gtggatgacg ccatcgtggt ggtggaaaac gtcgaacgta ttatgagtga ggaaggactc
    12961 actcctcgcg aagccacacg taaatcgatg gggcagatcc agggggcact ggtcgggatt
    13021 gcgatggtgc tttcggcggt atttgtacca atggccttct tcggcggcac caccggtgcc
    13081 atctatcgcc agttctctat taccattgtt gcggcgatgg tgctgtcagt actggtagcg
    13141 atgctcctca ctccggctct gtgtgccacg ctacttaagc cactgaaaaa aggtgagcat
    13201 cacgggcaaa aaggtttttt tgcctggttt aaccagatgt ttaaccgcaa cgccgaacgc
    13261 tacgaaaaag gggtggcgaa aattctccac cgtagcctgc gctggattgt gatttatgtc
    13321 ctgctgcttg gcggcatggt gttcctgttc ctgcgtttgc cgacgtcgtt cttaccgctg
    13381 gaagaccggg gcatgtttac tacctcggta caattgccca gcggttcaac ccaacaacag
    13441 accctgaaag tcgttgagca aatcgagaaa tattacttca cccatgaaaa agacaacatc
    13501 atgtcggtgt ttgccaccgt tggttctggc cctgggggta acgggcaaaa cgtggcgaga
    13561 atgtttatcc gcctgaaaga ctggagcgaa cgcgacagta agaccggcac ctcgtttgcc
    13621 attatcgagc gtgcaacgaa ggcgtttaac caaattaaag aagctcgcgt tatcgccagc
    13681 agcccaccag caattagcgg tcttggtagt tctgcaggtt ttgatatgga gttgcaggac
    13741 cacgctggag cgggtcacga tgcgctgatg gcagcacgta atcagttgct ggcgctggcg
    13801 gcggaaaacc cggagctaac ccgtgtgcgc cataacggcc tcgatgacag tccgcagttg
    13861 cagattgata tcgaccagcg taaagctcag gcgctgggcg ttgatatcga cgatattaac
    13921 gacacactgc aaaccgcctg gggttcgagc tatgtgaatg actttatgga tcgcggtcgc
    13981 gtgaagaaag tctatgtgca ggcagctgcg ccgtatcgca tgctgccaga tgacatcaat
    14041 ctctggtatg tccgaaataa agatggcggc atggtgccct tctctgcttt cgcgacctca
    14101 cgctgggaaa caggctcgcc gcgtctggaa cgctataacg gttattctgc ggttgagatt
    14161 gttggggaag ccgcaccggg ggtcagtacc ggtacggcga tggatattat ggaatcgtta
    14221 gtgaagcagc tgccaaacgg ctttggtctg gagtggacgg cgatgtcgta tcaggagcgg
    14281 ctttccggcg cgcaggctcc ggcgctgtac gccatttcct tgctggtggt attcctgtgt
    14341 ctggcggcat tgtatgaaag ctggtcggta ccgttctcgg taatgctggt cgtgccgctg
    14401 ggggtaatcg gcgcgctgct ggcaacctgg atgcgcgggc tggaaaacga cgtttacttc
    14461 caggtgggcc tgttaacggt cattggttta tcggcgaaaa acgccatcct gatcgtcgag
    14521 tttgctaacg agatgaacca aaaaggccat gacctgtttg aagcgacgct ccacgcctgc
    14581 cgtcagcgtt tacgcccgat tctgatgacc tcgctggcat ttatcttcgg cgtattgcca
    14641 atggcaacca gcacgggtgc cggttccggt ggtcagcatg cggtgggtac tggcgtaatg
    14701 ggcgggatga tttcagctac tattctggct atttacttcg tgccgctgtt ctttgtgctg
    14761 gtgcgccgcc gcttcccgct gaagccgcgc ccggaataag caataaaaaa ggcgacatgc
    14821 caatgtgtcg cctttttcaa ctttccgatt aagaacctgc tcagcgggtt cttgctgttt
    14881 atactttgtc tcaggaatta cttacgaagc ataacttcga taaagtcttt ccagttcccc
    14941 agttcacgtt caatcataac aacctctctt ataattatgg gtattctacg gaaacaatat
    15001 accgtggtga agctaattta ctcgattgct gcgatgacta cctccggggg acaaacctta
    15061 tgtaaatact atggtcctgc ggtgataatt tgtatgtgat acacagcaac atttcgagat
    15121 attcatacgg catctaatac tgatttaatt ctggttaaaa tacagacaga taacaagatg
    15181 aatattctta atgtttac
//
LOCUS       DAZPEU010000086        15135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPEU010000086 DAZPEU010000000
VERSION     DAZPEU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.086"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..539
                     /locus_tag="ACHU2L_002396"
     CDS             <1..539
                     /locus_tag="ACHU2L_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357227.1"
                     /translation="PADGKEPIIERIFRLVERYPSRNAAARAWGINEGTLKNYYNRRD
                     YAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDAQILMFLSFL
                     KPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLPDEKVREIYA
                     LSEAKGDHLNLPQKAASA"
     gene            complement(812..1759)
                     /locus_tag="ACHU2L_002397"
                     /pseudo
     CDS             complement(812..1759)
                     /locus_tag="ACHU2L_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(1759..2352)
                     /locus_tag="ACHU2L_002398"
     CDS             complement(1759..2352)
                     /locus_tag="ACHU2L_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR1357228.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            2499..2906
                     /locus_tag="ACHU2L_002399"
     CDS             2499..2906
                     /locus_tag="ACHU2L_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR1357229.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(3026..4018)
                     /gene="rpoS"
                     /locus_tag="ACHU2L_002400"
     CDS             complement(3026..4018)
                     /gene="rpoS"
                     /locus_tag="ACHU2L_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR1357230.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(4081..5175)
                     /gene="nlpD"
                     /locus_tag="ACHU2L_002401"
     CDS             complement(4081..5175)
                     /gene="nlpD"
                     /locus_tag="ACHU2L_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR1357231.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(5360..5986)
                     /gene="pcm"
                     /locus_tag="ACHU2L_002402"
     CDS             complement(5360..5986)
                     /gene="pcm"
                     /locus_tag="ACHU2L_002402"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR1357232.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(5980..6741)
                     /gene="surE"
                     /locus_tag="ACHU2L_002403"
     CDS             complement(5980..6741)
                     /gene="surE"
                     /locus_tag="ACHU2L_002403"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR1357233.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(6722..7771)
                     /gene="truD"
                     /locus_tag="ACHU2L_002404"
     CDS             complement(6722..7771)
                     /gene="truD"
                     /locus_tag="ACHU2L_002404"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR1357234.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(7768..8247)
                     /gene="ispF"
                     /locus_tag="ACHU2L_002405"
     CDS             complement(7768..8247)
                     /gene="ispF"
                     /locus_tag="ACHU2L_002405"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR1357235.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(8247..8957)
                     /gene="ispD"
                     /locus_tag="ACHU2L_002406"
     CDS             complement(8247..8957)
                     /gene="ispD"
                     /locus_tag="ACHU2L_002406"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR1357236.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(8976..9287)
                     /gene="ftsB"
                     /locus_tag="ACHU2L_002407"
     CDS             complement(8976..9287)
                     /gene="ftsB"
                     /locus_tag="ACHU2L_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR1357237.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(9481..9804)
                     /locus_tag="ACHU2L_002408"
     CDS             complement(9481..9804)
                     /locus_tag="ACHU2L_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR1357238.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(9854..10459)
                     /gene="cysC"
                     /locus_tag="ACHU2L_002409"
     CDS             complement(9854..10459)
                     /gene="cysC"
                     /locus_tag="ACHU2L_002409"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR1357239.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(10459..11886)
                     /gene="cysN"
                     /locus_tag="ACHU2L_002410"
     CDS             complement(10459..11886)
                     /gene="cysN"
                     /locus_tag="ACHU2L_002410"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR1357240.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(11888..12796)
                     /gene="cysD"
                     /locus_tag="ACHU2L_002411"
     CDS             complement(11888..12796)
                     /gene="cysD"
                     /locus_tag="ACHU2L_002411"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR1357241.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            13048..14085
                     /gene="iap"
                     /locus_tag="ACHU2L_002412"
     CDS             13048..14085
                     /gene="iap"
                     /locus_tag="ACHU2L_002412"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR1357242.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(14231..15135)
                     /locus_tag="ACHU2L_002413"
                     /pseudo
     CDS             complement(14231..15135)
                     /locus_tag="ACHU2L_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
BASE COUNT         3687 a         4054 c         3749 g         3645 t
ORIGIN      
        1 ttcctgccga tggaaaagaa ccgattatcg aaagaatctt taggttagta gaacgatacc
       61 cttcaagaaa tgcggctgct cgggcatggg ggatcaatga aggcacttta aaaaattatt
      121 acaacaggcg ggattacgca ccgataccac gcccgtatca gttaaaaaag atagcagaaa
      181 gcgaaaatgt taccttggaa tggctcatga cgggagaggg tgatgttgaa ctaaaagatt
      241 ccaaagaacc caaaaaagag aatgcggata atgttgatgc tcaaattcta atgttcttgt
      301 catttcttaa accacaagaa aaacaacaag tagctgatgt tcttgggaga aaaggagcgg
      361 agcaattact cattcttctg gatgagcaca tccaagagtt gcacgctcta gttggagttc
      421 gtagagccat agcgttaagc cttggtaatt tgccagacga aaaggttaga gagatttacg
      481 cgttgtccga agctaagggc gatcacttaa atctacctca gaaagcagcc agcgcataac
      541 ccagtgcaag tgcctagctg tgctaaaatt acattggttt tggcgcttgc acccatcagc
      601 gctataataa acacctgaat cgtttttgaa cggtctttaa aggctattgt aagaacctgt
      661 gagttatctt gaatgtttta ttgatatcga accgtttaac atttttggac tcgctttatc
      721 ttgaaaacct tgagttccta acttgttgat tccaatctat tcccgtcttt tttaatctct
      781 tcccggttat cttgaataaa ttacctagtc ataggtagtc gatttcggtc cacggcatac
      841 cgagatagag tgattcgaaa atcggtttgc tgcgataaga gactttatcg atgcgcacca
      901 cggtcatgtt gcgcccaccg gagtagtgcc cggtaaactc accgaacggc ccttcgattt
      961 cgcgtttacg gctttcgata accccttcga ggatcacttc tgaaccccac ggcacatcaa
     1021 aaccggtcag cggagcggtg gcgatcgggt acgggctttc acgcagcgcg cctgccattt
     1081 catactcaga ctgatcgtat ttcagcggcg tggcccccat cagcgtgatg atcggatcgt
     1141 taccgagcgt gatcgcaatc ggcagatctt caccgcgctc ttctgcttta tgcagatgca
     1201 gggcgatatc gtgcatcggc accggttgca ggccgagctt acgcttgccc ttcacttcca
     1261 tgcggtagat gccgacgttc tgcttgccga agttatccgg gtcgagcgga tcgcgggaaa
     1321 ccacgcacgc tttgtcgaga tagaaaccgc catcgccatc attcaggcga aacagcggca
     1381 gaatatcgaa caggttgatc tcttcgccat cgacggtgtt ctgcgcccag gctggattgg
     1441 cgcggcgctc cggggcaatc gggaagttat cccagcggcg gataaactca tcaatctgct
     1501 ttttgaccgg ggtgtttggt ggcaggccaa gggaaatcgc gtggttctgc caggaaccga
     1561 tggtattcat cgccacgcgg gcatcggtaa agccgcgaat attatcaaac cacagcgcgg
     1621 gcgcgccgtc gccgatacgc ccggtggcgt tagctgctgc tgccagatcc ggctcagcgt
     1681 tcacttcttc gctgattttc agtaactggc cgtggtcatc aagcgcctgt aaaaagctgc
     1741 gtaaatcatc aaatgccatt attcattctc ctgagaaaaa ttccgggcct gcggcaatcc
     1801 ttgccagcgc ctggcgtggg gatgttcgag gccaaattga tccagcacgc gggctaccac
     1861 atggtggaca atgtcatcta ccgtttcggg atggttataa aaggcaggca tcggcggcac
     1921 catcgccacg cccatgcgtg aaagtgcgag catattttcg agatggatgg tgctaagcgg
     1981 catttcacgc ggcaccagca ccagtttgcg gccttctttg agcacgacgt ccgccgcgcg
     2041 ccctaccagg ccatcggcgt aaccagcgcg gataccggcg agcgttttca tactgcacgg
     2101 aataacgatc atgccgtcgg tacgaaaaga accggatgag atgatcgccg cctgatccgc
     2161 cgggttatgg ctgaagtctg cgagggcagc aacatcgcga gcgctgtaag gcgtttccag
     2221 ttcaatggtg gttttcgccc acttcgacat caccagatga gtctcgacat tcggcatctc
     2281 acgcagcgct tgcagtaatg ccacaccaag aggcgcaccg gtagcccctg tcatcccgac
     2341 gatcagtttc attgcttatt ctcttaatta ttcgtatacg aacgttatta ttaacatact
     2401 ccttcatttc cacagcggca aggattgaaa agtaacaaca tcttcttcgt cactaaaaaa
     2461 ccgctatgat agcgacagag aattagcccg gagtttacat ggcgttacga aataaagcgt
     2521 tccatcagtt acgacagctt tttcagcagc acacggctcg ctggcagcac gagttacctg
     2581 acctgaccaa accacagtac gcggtgatgc gcgccattgc tgataaacct ggtattgaac
     2641 aggtggcact gatagaagca gcagtcagca ccaaagcaac gctagcagaa atgttggcaa
     2701 gaatggaaaa tcgtggctta gtcagacgtg aacatgacgt cgctgacaag cggcgacgct
     2761 ttgtctggct gactgccgaa ggagaaaagc tacttgctgc ggcgatacct attggtgaca
     2821 gcgtggatga ggaatttttg gggcgtttga gtggagaaga gcaggagctg tttatgcagt
     2881 tggtgcgcaa gatgatgagc acatagaatg ctgattgccg ggtaggacgc tgacgtgtct
     2941 tatccaggcg acaaaaacac aagcttccca aacaaaagaa aaaggccagc ctcgcttgag
     3001 actggccttt ctgacagatg cttacttact tgcggaacag cgcttcgata ttcagcccct
     3061 gcgtttgcag gatttcgcgc aaacggcgca ggccttcaac ctgaatctgg cgaacacgtt
     3121 cacgggtgag gccaatttca cgacctacat cttccagtgt tgccgcttca taccccagca
     3181 aaccgaatcg acgtgccagt acttcacgct gtttggcgtt cagctcgaac agccatttga
     3241 cgatgctctg cttcatatcg tcatcttgcg tggtatcttc cggaccgttc tctttttcat
     3301 cggccaggat gtccagcaac gctttttcgg aatcaccacc cagcggggtg tctaccgagg
     3361 taatgcgctc gttaagacga agcatacggc tgacgtcatc aactggctta tccagttgct
     3421 ctgcgatctc ttccgcactt ggttcatggt ccagcttatg ggacaactca cgtgcggttc
     3481 gcaggtaaac gttcagctcc tttacgatgt gaatcggcaa acgaatagta cgggtttggt
     3541 tcataatcgc ccgttcaatc gtctggcgaa tccaccaggt tgcgtatgtt gagaagcgga
     3601 aaccacgttc cgggtcaaac ttctctaccg cgcggatcag ccccaagttg ccctcttcga
     3661 taaggtccag caacgccaga ccacgattgc cataacggcg ggcaattttt accaccagac
     3721 gcaagttact ctcgatcatc cggcggcgag aggcgacatc tccacgcagt gcgcgacgcg
     3781 caaaataaac ttcttcttcg gccgttaaca gtggtgaata accaatctca ccaaggtaaa
     3841 gctgagtcgc gtccaacaca cgctgtgtgg ctccctgcga taacagttcc tcttcggcca
     3901 aatcgttatc actgggttcc tcttctacta aggccttttc gtcaaaaacc tcaactccgt
     3961 tctcatcaaa ttccgcatct tcatttaaat catgaacttt cagcgtattc tgactcataa
     4021 ggtggctcct acccgtgatc ccttgacgga acattcaagc aaaagcctgg ttccgccgat
     4081 ttatcgctgc ggcaaataac gcagcgggtt tacggatttc cccttgtaac gaatttcaaa
     4141 atgcaagcgt gttgaactgg ttccggtgct acccatggtt gctatttttt gccccgcctt
     4201 cacttcttgt tgttcccgga ccagcattgt gtcgttatgg gcgtaggcac tcaggtaatc
     4261 atcattatgt ttgatgataa tcagattacc gtagccgcgc agcgcgttac cagcataaac
     4321 aacgcggcca tctgcggtcg cgataattgc ctgtcctttg ctgcctgcga tatcaatccc
     4381 cttgttgccc ccctcagaag cgccaaaggt ttcgatcact ttgccctcag tcggccagcg
     4441 ccaggtggag ataggcgtac tggttgatgt actgctgaca gtcggctcgg ttgtgcttgc
     4501 tgttggtacc gttacaggcg ctgtgaccgt ggtcgcagtt ggcttgttgt tcggcaacat
     4561 tttgttagca ctctgttcac ccgaagactc agaatacgta attgtcggtt gcgacgcaac
     4621 agcaacggtg gaattttgtg caggcttgat cacaactcct tgctctgctg cgtcggcctg
     4681 ggtaatggca tttccgccag tgattggcgt accggaagca ttacccacct gcaaggtctg
     4741 accaacgttc agcgcgtatg gtgcctgaat attgttgcgc tgagcaaggt cacggaaatc
     4801 gttgccagta atccaggcga tatagaaaag tgtgtcgcct tttttcacgg tataggtact
     4861 gccgctataa ctgcctttcg gaatgttccc atactgacgg ttatagacga tgcgtccgtt
     4921 ttccatctgt accggctgct gagctactgg ctgcactggc tggatttgcg gttgttgagt
     4981 agcctgaatt tgtggctgct gtaccggctg aatttgcggt tgctgcgctg tagacgtcgt
     5041 ccccattttc ggcggcggcg taatcaacat accagaatta gtatttgcag gcgcattgcc
     5101 attaacggag ctgaccggtg ccggtggatt tgaagtgtca gaacagcctg ccagccatag
     5161 cgaaaccagt gacaaagccg caatgcggcg aacggtgaat tttgggcttc ccgcgctcat
     5221 ttatccccca ggaaaaattg gttaataacc agtgacataa ttaccgtgca aggcacccta
     5281 ctgaacactg gaaaagatgt tcacgatacg ctgacctacg gcaaaataac caggaaaaat
     5341 ccaggtattt cctcacgttt taagccagct cacccttcac taaagggaca aagcgcacgg
     5401 cctccacggt atcgataata aattcgcctc cccgacgacg cacccgtttc aaatactggt
     5461 gctcctcccc tacgggtaag acgagaatcc cgccttcgtc cagctgcgtc attagcgcag
     5521 ttggaatttc cggcggtgcc gccgtaacaa tgatagcgtc aaacggcgca cgtgcctgcc
     5581 aaccttgcca tccatcacca tgacgggttg aaacattatg taaatcaaga tttttcaggc
     5641 ggcgacgtgc ctgccactgc aagcctttaa tccgttcaac cgagcaaaca tgctggacaa
     5701 gatgcgccag gattgccgtt tgatatcccg aaccagtgcc aatttccagc acccgcgact
     5761 gcggcgtcag ctcgagtaat tcggtcattc gcgccaccat atacggctgc gaaattgtct
     5821 gcccctgacc tatcggcaag gcgatattgt cccaggcttt ttgttcaaac gcttcatcaa
     5881 cgaatttttc acgcggcacg gcggcaagtg cattcagcac ctgctcatcc tgaatacctt
     5941 gcgcacgtaa ttgatccaga agtgcttgta cgcgtctgct taccattgcg tgccaactcc
     6001 cacgctgttt aaccagtctg aaaccacatc ttgcgcgcta tgcgcagtta aatccacatg
     6061 cagcggcgtg atggagacat agccctcatc taccgcagca aaatcggtcc ccggaccggc
     6121 atcacattta ccgcccggcg ggccaatcca gtacagcgta ttgccgcgcg gatcttgctg
     6181 cgggatcacc tgatctgccg gatgtcgtgt accgcagcgc gtcacgcgaa tacctttgat
     6241 ttgatccaag ggtaaatcct gaacgttaat attaagaata cgtccggtgc gcagcggctc
     6301 tttacacagt gcgcgcaaaa ttgaacaggt taccgccgcg gcagtgtcgt aatgtttatg
     6361 cccgtcaagc gagacggcca gcgccggaaa acctaaatga cggccttcca tcgcggcggc
     6421 taccgtaccg gaataaataa catcatcccc cagattcggc ccggcgttaa ttccggacac
     6481 aacaatgtcc gggcgcggac gcatcagagc attcacgcca agatagacac aatcggtcgg
     6541 ggttcccatt tgcacagcaa tatcaccatt ttcaaaggta aacgtgcgca gggaggattc
     6601 cagtgtcaga gaatttgaag cgccgctgcg gttacgatcg ggggcgacca cctgaacgtc
     6661 agcaaactca cgcaaggctt tcgccagcgt ttgtataccg ggtgcatgta ccccgtcata
     6721 attactcagc aatatgcgca taatcacctg ttgtgttgat aagttccctg acaacgctgg
     6781 ttgcaaaact acccgccgga agccagaaac ggatctctac ggtgacgtca tcccaccagt
     6841 tccagcttaa ttgttgcgga tacagcagca tcgctctgcg cgcggcttca actttttcgc
     6901 gcaccagtaa agcttgtaat tcagtttctg cggcgacagc tgcttgttcg aatgccagcg
     6961 cttcacgctg agttccccat tcgccactgc ccggcaatgc ggcggttatc atcagctctt
     7021 tatcgttgac gcgacgctgt aattccgcca gttcttcggt ggttgctaca aaccagctac
     7081 cacgtccggc taattgtagc gcatcgccgt caacaacttg attaacgtct gcttttttga
     7141 ggcgctcagc aacaatctga ttaaacaacg cactgcgggc tgccgacaac caaaaactcc
     7201 gtttattgcg atcgcgcacc ggagtattgg tttgcgccca gcgcagcgcg ccctgcaagt
     7261 tgctaccgcc aatcccaaaa cgttgggcac cgaagtagtt cggtacacct tttacgcaaa
     7321 tatcgatcag acgttgttca acgtcatcgc gattgctcac ttcgcgcaga accagggtaa
     7381 aggcgttacc tttcagcgcc cctaaacgca gcttgcgctt gtgccgcgca tactccagca
     7441 cctggcagcc ttccagttga aaggcgctca gatcgggcat ttccttgccc ggcacgcgag
     7501 cacataacca ctgttccgtt acagcatgtt tgtctttttg cccagcgaag ctgacttcac
     7561 gggaatgaat tttcaggaat ttcgccagtg catccgccac aaaacgggta ttgcagccgt
     7621 ttttgaggat tctaaccaga atatgctcac cttcaccatc aggctcaaag cccaaatctt
     7681 ccaccaccac aaagtcttcc ggattggctt tcagcagccc ggtgccttgc ggtttaccgt
     7741 ggaggtaagt gagattatca aactcaatca ttttgttgcc ttaatgagta gcgccaccgc
     7801 ttcacaggca atcccttccc cacgtccggt aaatccaagt ttttccgtag tagtggcttt
     7861 cacgttaaca tcatccatat ggcagccgag atcttcggca ataaacacgc gcatttgtgg
     7921 aatgtgcggc aacatcttcg gtgcctgagc gatgatagtg acatcgacgt tgccaagggt
     7981 ataacccttc gcctgaatac gacgccaggc ttcgcgtagc agctcgcggc tgtcggcacc
     8041 tttaaatgcc ggatcggtat ccgggaacag tttgccgata tcccccagcg ccgccgcgcc
     8101 aagcaatgca tcggtcaacg catgaagcgc cacgtcgcca tcagaatgcg ccagcaatcc
     8161 tttttcgtaa ggaatgcgta cgccaccaat gataattggg ccttcaccgc caaaggcgtg
     8221 tacgtcaaaa ccgtgtccaa ttcgcattat gtattctcct gatggatggt tcgggtgagg
     8281 taaaactcgg ccagtgccaa atcttccggg cgcgtgactt taatgttatc cgcgcggcct
     8341 tcgaccaact gcggatgaaa tccgcaatat tccagcgccg aggcttcgtc ggtaatggta
     8401 gcgccttcat ttagagcgcg cgtcagacag tcatgtaaca gctcacgagg gaaaaattgc
     8461 ggcgtcagcg cgtgccataa gccgttgcga tcaacggtat gagcaatggc atttttgccc
     8521 ggttcggcac gtttcatagt atcgcgcact ggtgcggcga ggatccctcc cgtgcggctg
     8581 gtttcgctca acgccaacaa tcgcgcaagg tcatcctgat gcaaacaagg acgagcggcg
     8641 tcatgcacca atacccactg cacatcgcca gcggctttca gacctgccag cacggaatcg
     8701 gcacgctcat caccgccatc tacaacggtg atttgcggat gattcgccag aggaagttgt
     8761 gcaaaacggc tatcgccagg acttatggca atgacgacac gtgtcacccg gggatgcgcc
     8821 agcagcgcat gcaccgagtg ttcaagaatg gtttgattac cgattgagag atattgctta
     8881 ggacattccg tttgcattcg acggccaaat ccggccgccg gaaccacggc gcaaacatcc
     8941 aaatgagtgg ttgccatgtt aattcccggg ctgatttatc gattgttttg ccccgcagac
     9001 tgtgcgcgct tcgacgcgtc aggcaccaga cgataaaaag tttcgcccgg cctggtcatg
     9061 ctgagttcat tacgcgcacg ctcttcgagc gcctcctggc cgccattgag atcgtcaatt
     9121 tcggcaaaaa gttgatcgtt tcgcgcttta agtttcgcgt ttgtagcttg cagtgccgcc
     9181 acatcatcat tgacgcgggt atagtcatgt ataccgttct taccgaacca cagcgaatac
     9241 tgtagccaga ccagaatagc cagcaacagc agcgttagtt tacccatcct gccccctgaa
     9301 aaactgcatc atcatcccat gcatccgaag acgactctac atcctctgtt ggggataccg
     9361 cgacaacgcg ggcaaatgta ccacatttgc ccattgttac gtatacccag ggcgtgcaga
     9421 acataatctc attattagtt acggtttgaa ttatgaacag aggagacaag aaagtacaaa
     9481 ttagcccagt agccacataa acagtgcgcc aaacataatg cctactgtca ccagggtgaa
     9541 aacaatactg tagcgtagct ttccgtccat caatgaatgc agcgcaatcc ccaccactac
     9601 cgcgacaggc atcagtgcca gaaagaaagg ccaggtgtag agaaagaaga acagcgtgtt
     9661 agagccataa atcaacatcg gcatcgccag cgcaaataac caggagataa aaccgaccac
     9721 ggcaccaggc agtgaccatg tagtttcttc atcctcagta aggctgtcgt tatttgttag
     9781 tgtaatgtta tggctattac gcatatttga tcctgttact ttgacgaacc gggcatggaa
     9841 acccggtggt gtctcaggat ctgataatat cgttctgtct caacagatct aataattgct
     9901 gtaccaaatt tgttactaat tgttcaccat tgagatgaat ttctgccgat tcaggcgctt
     9961 cgtaaacgga atctattccc gtaaagttgc gcagttcacc ggcacgcgct ttcttatata
    10021 agccttttgg atcgcgggct tcgcaaatcg ccagcggcgt atcgacaaac acttcaataa
    10081 agcgcccttc tcctacgcgt tcgcgaacca tctggcgttc ggcgcggtgt ggcgagataa
    10141 atgcggtcag caccaccagt ccggcttcaa ccatcaaatt cgccacttca ccgacgcgac
    10201 ggatattctc tttacgatcg gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa
    10261 cattgtcgcc atccagcaga tacgtactga cgctgagttt atgtaacgcc tcctccagcg
    10321 ccccggcgac cgttgattta ccggacccgg agaggccggt aaaccacagc actacaccac
    10381 gatgaccgtg gtgtagttcg cgttgttgca cagtgaccgg atgactatgc cagacgacgt
    10441 tttcgtcatg cagcgccatt atttatcccc cagcaaatcg cgcgcgcccc agtgcggaaa
    10501 gtggcgacga accagggcat tcaattccag ttcgaatgca ctgaattcag atgacgcagc
    10561 tgttgcctgg ctaactggct cgtgcaccat accggcaccc acggtcacat tgctcaggcg
    10621 atcgataaaa atcagcccac cggtaaccgg attttgctga tagcgatcta acaccagtgg
    10681 ctcgtcaaaa gtgagatcca ccaggccgat gccgttcagc ggcaggtttt caacttcgcg
    10741 ttgggtaagg ttattaatat caacctgata acgaatgcca tcaacacgag cacgcgtctt
    10801 cttaccggca attttgatgt cataactctg gcccggggaa agcggctgtt ccgccatcca
    10861 taccacatcc accgacgcac tctgcaccgc cggtaacgct tcgtctgccg ccagcagcag
    10921 atcaccacgg ctgatatcga tctcatccgt cagcaccagg gtgatcgctt ctccggcaaa
    10981 gacttcttcg cgatcaccat caaaagtcac gatccgcgcg acgtttgatt ccacaccaga
    11041 gggcagcact tttacacgtt gcccgacttc cacgcgaccg gatgccagca ttccggcgta
    11101 gccacgaaaa tcgagatttg ggcggttaac gtactgcacc gggaagcgca ttggctgggc
    11161 atccaccact cgctggatct ccacggtttc cagcacttcg agcagtgtca gaccgctgta
    11221 ccacggcata ctttcacttt gcgaagccac gttgtcacct tccagtgcgg agagtggcac
    11281 aaagcggata tccagattac ccggcagctg cccggcaaag gtcagataat cttcacgaat
    11341 acgggtgaac gtctcttcac tgtaatccac cagatccatt ttgttgatcg ccacgaccag
    11401 atgtttgatc cccaacagtg tggagataaa actgtgacga cgggtttgat cgagcacgcc
    11461 tttacgggca tcaatcagta agatcgccag ttcacatgtc gatgcgccag tcgccatatt
    11521 gcgagtgtac tgctcgtgcc ctggggtgtc ggcgataata aatttacgct tctcggtaga
    11581 gaaatagcgg taggccacat caatagtgat gccctgttcg cgctcagctt gcaggccgtc
    11641 caccagcaga gccagatcca gcttttcgcc ctgggttccg tgacgcttac tgtcgttatg
    11701 cagcgatgag agctgatctt cgtagatttg gcgggtatcg tgcagcagac gaccaatcag
    11761 ggtacttttg ccgtcatcga cgctaccaca ggtcagaaaa cgcagcaggc ttttatgttg
    11821 ttgcgcaatc atccaggctt cgacgccgcc ttcattggcg atttgttgtg caagtacggt
    11881 gttcatctta aaaatacccc tgacgttttt tcagctccat ggatcccgcc tggtcgcggt
    11941 caatcacgcg accctgacgt tcactggtgg tggaaaccag catctcttcg atgatttccg
    12001 gcagtgtttg tgcatttgac tccaccgcac cggtcagcgg ccagcagccc agcgtacgga
    12061 aacgcaccat ccgttttttg atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa
    12121 tcatcatcaa cataccgtcg cgttccagaa ccggacgttc cgcagcgaga tacagcggca
    12181 caatgtcgat attttccagc cagatgtatt gccagatatc ctgctcggtc cagttagaga
    12241 gcgggaagac gcggatgctt tcgcctttgt taatttgccc gttgtagttg tgccacagct
    12301 ccgggcgctg atttttcgga tcccagcgat ggaagcggtc acggaaagag tagattcgct
    12361 ctttagcgcg ggatttctct tcgtcacggc gcgcaccacc gaaggcggca tcaaaaccgt
    12421 atttgttcag cgcctgtttc aggccttcag ttttcataat atcggtatgt ttcgcgctgc
    12481 cgtgcacgaa tggattaatc cccatcgcca cgccttccgg gtttttatgc accagcagct
    12541 cgcagccgta ggctttcgcc gtacgatcgc ggaactcata catctcgcgg aatttccagc
    12601 cggtatcgac atgcagcaac gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca
    12661 gatgcagcat gacgctggaa tctttaccga tagagtagag catcaccgga tttgagaatt
    12721 ctgccgccac ctcgcgaata atgtggatgc tttccgcctc cagttgccgc aggtgagtaa
    12781 gtcgtttttg agccataacc gttcctttgc aataccgcta ttttcttgcc atcagatgtt
    12841 tcgactatag ggagcgtaag agaacgaatg aaattaccaa ttagaatgag tagttcctta
    12901 acggaataac gatttggcaa agctaatatc aaaaagtgct taaggcaccg gatttcgggc
    12961 gtttaggaag atttgaaatt gttttagcgc agcggcagtt tcatactatg gcggtaaaaa
    13021 aatttgcatg gtatttaagg actcactatg ttttccgcat tgcgccaccg taccgctgcc
    13081 ctggcgctcg gcgtatgctt tattctcccc gtacacgcct cgtcacctaa acctggcgat
    13141 tttgctaata cccaggcacg acatattgct actttctttc cgggacgcat gaccggaact
    13201 ccagcagaaa tgttatctgc cgattatatt cgccaacagt ttcagcaaat gggttatcgc
    13261 agtgatattc ggacatttaa tagtcggtat atttataccg cccgcgataa tcgcaagaac
    13321 tggcataacg tgacaggaag tacggtgatt gccgctcatg aaggcaaagc gccgcagcag
    13381 atcatcatta tggcgcatct ggatacttac gccccgctga gcgatgctga cgcagatgcc
    13441 aatctcggcg gactgacgtt acaaggaata gatgataacg ccgcaggttt aggtgtcatg
    13501 ctggaactgg cagaacgcct gaaaaatacg cctaccgagt atggtattcg atttgtggcg
    13561 accagcggcg aagaggaagg aaaattaggc gctgagaatt tactcaagcg gatgagtgac
    13621 accgaaaaga aaaatacgct gctggtgatt aatctcgata acttaattgt tggcgataaa
    13681 ttgtatttca acagcggtgt aaaaacccct gaagcagtaa ggaaattaac gcgcgacagg
    13741 gcgctggcaa ttgcacgcag tcacggaata gccgcaacga ccaatccggg tttgaataaa
    13801 aattatccga aaggcactgg gggttgtaat gacgcagaaa tattcgacaa agcgggcatt
    13861 gctgtacttt cggtggaagc gactaactgg aatcttggaa ataaggatgg ttatcagcaa
    13921 cgcgcaaaaa cacctgcctt cccggcggga aatagctggc atgacgtaag actggataat
    13981 caacaacata ttgataaggc acttcctgga agaatagaac gtcgctgccg tgacgttatg
    14041 cggataatgc taccgttggt gaaggagctg gcgaaggcgt cttgatgggt ttgaaaatgg
    14101 gagctgggag ttctaccgca ggggcgggga attctaagtg atacccatca tcgcatccag
    14161 tgcgtccggt ttacttccgg gaaaaccgca ccgtattgcg ccggtttatc tccgctggag
    14221 cggggaacac ctctggcgaa tagaaagcaa gccaggtttt gaccagtcga tcgtacatgg
    14281 cattggccgt tgccattgtt ggcaggccaa aaaacagccc ctgagcgtta cccgcagcca
    14341 tcaacctgtg cgccaggata agcgccgctt ctgttttacc tgcgccggtc acatcttcca
    14401 gaataaacaa ctgtggtcct ggctgattga tatccagatc cagcaccttt tgctgtaacg
    14461 gtgtcgtatt ctcaataaaa ggaaacagcg tattaattcc ggtgaaaggc gcggtttctg
    14521 cttgtggcgg aaagacggtt aatgcgtttt gagcctgaac taaagccttt tgccagtaat
    14581 cttcaatatc cattgcctgt gcgacgcgtg gaaaaaatcg cgttgacgac cctgtccagt
    14641 ctgcaagtac gacggttgca gagatatacc aggaaagttg ttttataagt tcaacgccct
    14701 cgtcatcatc ccagaatgtg gggatctcta ttcgcggaaa cagtggcttg atttcaagga
    14761 gaaaatctcg tgctgcagct ttgtcttcag gcagaaaatt atccaattca tccatacgat
    14821 cagggggccg accatgatgc cctgtcgtca cgggcatcca catttctatc actcgcctaa
    14881 gtttgcgcgg agagagcgaa gatgaaggaa gtaactcctg acattcactt aaataatgat
    14941 tccacaacca gtagcccagc gttgaatgag agattttttc tggcaccttc ctgaatcttg
    15001 aattcagggg gcaggtaaag ttgttgaaaa gagcgggcaa attttccaat atcgtgccag
    15061 cacagcaacc aggcgaaaaa tcgagctgcc tgttccttgt cagaaatccc taattgacga
    15121 aaataatcag ccagc
//
LOCUS       DAZPEU010000087        15052 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPEU010000087 DAZPEU010000000
VERSION     DAZPEU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.087"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..179
                     /gene="yjbD"
                     /locus_tag="ACHU2L_002414"
     CDS             <1..179
                     /gene="yjbD"
                     /locus_tag="ACHU2L_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR1357243.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(312..1184)
                     /gene="rluF"
                     /locus_tag="ACHU2L_002415"
     CDS             complement(312..1184)
                     /gene="rluF"
                     /locus_tag="ACHU2L_002415"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR1357244.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1532..2479
                     /locus_tag="ACHU2L_002416"
     CDS             1532..2479
                     /locus_tag="ACHU2L_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR1357245.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2549..3355
                     /locus_tag="ACHU2L_002417"
     CDS             2549..3355
                     /locus_tag="ACHU2L_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1357246.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3365..3772
                     /locus_tag="ACHU2L_002418"
     CDS             3365..3772
                     /locus_tag="ACHU2L_002418"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR1357247.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3772..4266
                     /locus_tag="ACHU2L_002419"
     CDS             3772..4266
                     /locus_tag="ACHU2L_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR1357248.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4332..5129
                     /locus_tag="ACHU2L_002420"
     CDS             4332..5129
                     /locus_tag="ACHU2L_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR1357249.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5140..5928
                     /locus_tag="ACHU2L_002421"
     CDS             5140..5928
                     /locus_tag="ACHU2L_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR1357250.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5980..7209
                     /gene="sorE"
                     /locus_tag="ACHU2L_002422"
     CDS             5980..7209
                     /gene="sorE"
                     /locus_tag="ACHU2L_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR1357251.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7591..8280
                     /gene="pepE"
                     /locus_tag="ACHU2L_002423"
     CDS             7591..8280
                     /gene="pepE"
                     /locus_tag="ACHU2L_002423"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR1357252.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8371..10002)
                     /locus_tag="ACHU2L_002424"
     CDS             complement(8371..10002)
                     /locus_tag="ACHU2L_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR1357253.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10222..13905)
                     /gene="metH"
                     /locus_tag="ACHU2L_002425"
     CDS             complement(10222..13905)
                     /gene="metH"
                     /locus_tag="ACHU2L_002425"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR1357254.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14105..14929
                     /gene="iclR"
                     /locus_tag="ACHU2L_002426"
     CDS             14105..14929
                     /gene="iclR"
                     /locus_tag="ACHU2L_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR1357255.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3567 a         3972 c         3939 g         3574 t
ORIGIN      
        1 gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat aaattaatgg
       61 tcgagcgtga ggctgaagcg aaaaaagccc gccaattgaa gaaaaagcag gcttataaac
      121 cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc aggcgctaat
      181 gaacaatcaa cgcctgagat tttcactcgt ctgtgtggac acaatcttcc cctcacccta
      241 accctctccc cgaaggggcg aggggactgt ccgagctctt tttggacgtt ttcatcagtc
      301 tgaggcgtta atcagcgtcc cttcttttta cgccccggcg aggtaaaacg cttaccgttg
      361 gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg
      421 cccgctgttt tcggtttggc cttcgcttta ggttttgcct cggaagagga attttcgatg
      481 agcttaaaga ggtcgattaa ctcatcgtcg gttaaatcgc gccactcccc cagcggaatg
      541 ccgcttaagc tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa
      601 tgctcgcaca tgcgccggat ctgacggttc agcccctgca ctagggtaat gcggaagaca
      661 aacggcgctt cttttttgac tttgcacttt ttcgtcaccg tcccgaggat cggcaccccc
      721 gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct
      781 ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg
      841 aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg
      901 ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aataccgacg
      961 ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg
     1021 tttactttca caatgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta
     1081 aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt
     1141 tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa
     1201 cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt
     1261 aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacga tgcaaatttg
     1321 cctggcaaaa taaggataat caacatatta cgaatcgctg cacacctgtg ctgatgacta
     1381 ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa
     1441 aatcgctttg aaaggccctc atgatggggt tatatcagat caagcggtga gaaagcgtat
     1501 ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat
     1561 tgtgaagatt gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga
     1621 actgggtatt taccgcacca ccatcagccg cttgcttaaa cgaggccgcg atcagggaat
     1681 ggtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa
     1741 gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac
     1801 tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg
     1861 cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca
     1921 ggcggggcaa tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact
     1981 cgaaagccgc tatcatgtaa acacattaac ctacagcgcg gcagcgaagc tgaaagggga
     2041 atcgcatctc gcggattttc cggctctgct ggataaccca ttaattcgta atgggatcat
     2101 gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggata tcgccctggt
     2161 gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga
     2221 agagagtgac gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga
     2281 tattcacggc gcaatggttg aaacgaatat gagcgaaaaa acgctctcta tcgaaatgaa
     2341 taaattaaag caggcgcgat attccattgg cattgccatg agtgaagaaa aatacagcgg
     2401 aattattggt gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc
     2461 tgaactatta ctgaaataaa aatcttcccg atgtttaccg taataaaaat tacggcgggt
     2521 ccctattatc taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat
     2581 cattattgtc accggcggcg catccggtat tggtctggcg attgtagagg aattattagc
     2641 acaaggcgcg aatgtacaga tggtcgatat tcacggtggc gatggtcaat atgaaggtca
     2701 taaaggctat cagttttggc cgaccgatat ttctagcaca aaagaggtaa atcatacggt
     2761 agcggaaatt atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa
     2821 tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc
     2881 tgcattcgaa aaaatggtca atatcaacca gaaaggtgtt tttctgatgt cgcaggcggt
     2941 ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg
     3001 gctggaaggc tcagaaggac aaagctgtta cgccgctacc aaagccgcgc tcaatagctt
     3061 cacgcgctcc tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc
     3121 cgggattctg gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac
     3181 gcgcaatatc accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg
     3241 gcgcgccgga agattagcag aagtagctga ttttgtttgt tatctgttgt ctgaacgcgc
     3301 cagctacatc accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg
     3361 ctttatggtc aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc
     3421 ggttcagatg gtttacggca acgccaatgt tgaagccgtt gcatttgtgc ccggtgaaaa
     3481 cgccggagac attgcggtaa agctggaaaa gttagtaagc attcacaatc aagatgagtg
     3541 gctgattgcc gtggatttac agtgcggcag cccgtggaat gcggcagcaa tgcttgcgat
     3601 gcgcaatcct cgtttgcggg taatcagcgg cctttctctg ccgctggcgc tcgaactggt
     3661 tgataaccag gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa
     3721 gcaaacctgc gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt
     3781 acgctcgccc gcattgatga ccgtctgatc cacggccagg tcactaccgt ctggtcgaaa
     3841 gtggctaacg cccagcgcat tattatctgc aatgacgaag tttataacga tgaagtgcgc
     3901 cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa
     3961 aaagccgtcg cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc
     4021 actcgcccac aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctgaat
     4081 attggcggca tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat
     4141 gatgatgata ttaacgcatt tcatgagtta aataatcttg gtgtcatact ggatttacgc
     4201 gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa taaacgaaca gttaatcgca
     4261 aattaaataa tagcgcctgt gtacatatgc tttaacaggc agggatgacg acacttaaaa
     4321 ggtgacaaat catggaaata agtaccctac aaatcattgc tatatttctt ttttcctgta
     4381 ttgccggaat gggcagcgtg ctggatgaat ttcagactca tcgcccgtta attgcctgta
     4441 cggtgattgg tttaattctc ggtgatttaa aaaccggaat aatactcggc ggtacgctgg
     4501 aattgatagc tctctgctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg
     4561 ccagcataat ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg
     4621 ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca
     4681 ccgtagtgtt ccagcacgcg gcggataaag cagcagaaga ggcgcggttt cgcactctcg
     4741 atattctgca tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga
     4801 ttgtctcact gttcgtcagc gccgatatgg tcagcaatat gttaaacgcc attcccgaat
     4861 ttgtgacccg aggactacag attgctggcg gttttatcgt ggtggtcggt tacgccatgg
     4921 tgctacgtat gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag
     4981 gtggctacct cgatctcagc ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg
     5041 tctacatcca gctaaatcca cagtggtgta aagctgaacc tcaactccag gccaccgcct
     5101 ccaccgccct tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca
     5161 cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt
     5221 gaacgtattc acgggctggg cttttgctac gacatgatcc ccgccatcaa gcgactttac
     5281 ccattaaaag aggatcaggt tgcggcgctc aggctacacc tggtgttctt caataccacg
     5341 ccagccgtat gtggcccggt catcggcgcg gaaattgatg acggtaccat caacggcatc
     5401 aaagtcggtc tgatgggacc attggcagga gttggcgatc cactggtctg gggaacgctg
     5461 cgcccgatta ccgccgcgct cggcgcatct ctggcacttt cgggcaacat tctcggcccg
     5521 ctgctgttct tctttatttt caacgcggtg cgtctggcga tgaagtggta tggcctacag
     5581 ctcggctttc gcaaaggggt gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa
     5641 ctcaccgaag gcgcgtcgat tctcggactg tttgtgatgg gcgtgctggt gaccaaatgg
     5701 acgtcaatca acgtaccgtt ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc
     5761 accatgaccg tgcagaacat tctcgaccaa ctttgccctg gtttgctggc gctcggtctg
     5821 acgctactaa tggttcgtct gctcaacaaa aaaattaacc cggtatggct gattttcgcc
     5881 ctgtttggct tagggattat cggcaatgct ctgggcttcc tgtcctgatt cttcgccccg
     5941 gcacgactgc cgaggccatc gctcaacatg aggtggttta tgaaaacaac agctctgcgt
     6001 ctttatggta aacgtgattt acgcctggaa acctttgacc ttcctgaaat gcaggaggat
     6061 gaaatcctcg caacggtggt cactgacagc ctgtgcctct cttcctggaa agaggccaat
     6121 ctgggtgaaa accataaaaa agtacccgac gatgtgacga ccaacccaat catcatcggc
     6181 cacgaatttt gcggcgatat tctggccgtg ggtaaaaagt ggcagcacaa attccagccg
     6241 ggtcagcgtt atgtgattca ggccaacctg caactccccg accgcccgga ttgccccggc
     6301 tactccttcc cgtgggtaga cggcgaagcg acgcatgtgg ttattcccaa cgaggtcatg
     6361 gaacaatatt gcctgctggc atacgacggc gaaacctatt ttgaaggctc actggttgaa
     6421 ccgctttcct gcgtgattgg cgcgttcaac gccaactatc atcttcagga aggtagttat
     6481 aaccacacga tggggattcg cccgcaaggg cacacgctga tcctcggcgg caccggacca
     6541 atgggactgt tggcgattga ttatgcgctg catggaccag ttaacccgtc gctgctcgtc
     6601 attaccgata ccgacaacga caaattgagt tatgcgcgca agcactatcc atcagaaccg
     6661 caaacactga ttcattatct caatgccacc gatgcagcat ttgatacgtt aatggcgctg
     6721 agtggcggtc acggcttcga tgatattttc gtctttgtgc ctaatgaagg actggtgact
     6781 ctcgcctctt ccttgctggc gacagatggt tgcctgaatt tcttcgccgg accgcaggat
     6841 aaacatttca gcgcgccaat taatttctac gatgtgcatt atgcatttac ccactacgtg
     6901 ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg tcaaactgat cgaagagaaa
     6961 aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc tgaatgccgc gggcgaaacc
     7021 acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg tgtataccgg gaaatacctg
     7081 ccgctgacgt cactcacgca gattcaggat caagcactgg cggcgattct ggcgcgtcat
     7141 caggggatct ggtccggtga ggtggagcaa tacctgctcg cccatgcaga ggcaatttcc
     7201 catgattaat cgcgctactt aactctgcat gtcgctggct ggtcgcccca gcaacttcgc
     7261 gaccggatgt gcggctgatt gctgaagcag cacaatttgc acgagaagga taaaaaatat
     7321 cagcgcctta aggggcgctc aaattggtgg caaagtacac attgtttatg ccggatgcgg
     7381 aatgaagcct tattcggtct acataatttt gaaaatccaa tatattgcag ttatctggta
     7441 ggccaggtaa gcgtagcgca tcaggcaatg ctgtgtttgt catcagtctc agcgcccggt
     7501 gcaatacgtg acatcgtgag ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc
     7561 ttccgtgatt tcttactaac cggagaagta atggaactgc ttttattgag taactcgacg
     7621 ctgccgggta aagcctggct ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt
     7681 cgccgctcag cggtgtttat ccctttcgct ggcgtaacgc agacctggga tgattacaca
     7741 gagaaaacgg ctgcggttct cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt
     7801 gtcgatcccg ttgccgcgat tgaaaatgcg gaaattgtga ttgtcggcgg cggtaatact
     7861 ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg ctccaattac tgacgtggtt
     7921 aaacgtggcg cgctgtatat tggctggagc gcaggcgcta accttgcttg cccaaccatt
     7981 cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct aaatctgttc
     8041 ccgttgcaaa tcaacccgca cttcaccaac gcgctgccgg aaggccataa aggtgaaacc
     8101 cgtgagcagc gtattcgcga actgctggtc gtcgcgccag aactgacgat tattggtctg
     8161 ccggaaggta actggatcac agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc
     8221 acttatgtgt ttaaggctgg tgaagaagcg gttccgctgg aagctggtca ccgtttttaa
     8281 gtgaattgcc ggatgcatga tccggcatct tgttcatagt gcctgatgcg acgcttgacg
     8341 cgtcttatca ggcctacagg attcacaata ttaatactcg tcccgtcctt catcttcatc
     8401 cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcaggc gctgcatatc
     8461 tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgcacgtttt gctgatgcag
     8521 gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat gtttgctgcg
     8581 acgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg ccagctttaa
     8641 attactgagc aattgctcat agagcgcatc cagttccttc aacccatcaa gcgaaaatgc
     8701 ccgccgtgct gccagcgatt tatcggcaat ttcgctgccc atgcgctcga cgatatcgga
     8761 ggcctgttca aggttgagcg acatttcgat gatctccgcc cagcggcgcg actcttcctc
     8821 cgccaactct tcttttggca tccgcgccag atacagctta atggcggtgt agagaacgtt
     8881 gatatcatct gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt
     8941 attcagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgcgcggc
     9001 gttcgccaga gcaagcgtgg gcgtgtccag cgcgctgaca tccagatgtt taggccgtag
     9061 ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc
     9121 aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga aatagatcac
     9181 cagttccgct tttggcaatg acagcttccc cattgtctct gccagcaaat ggacaaacgg
     9241 caggataatc aggctaccca ccagcttaaa cagcagacta cccagcgcga cacggcgggc
     9301 tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggttagctcc
     9361 aatcaccaga cagagcgcca cggggaagga gataatgcct gcagcggtca gagtcgcggt
     9421 cagcagtaca gcagcaaggc tggagtagct gataatggcg aacatcgcgc caatcagcgc
     9481 atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt ttgcctgggt
     9541 gatcggcgtt acggcctgca caatcaactc cagcgccagc aaaatcagcc caagaccaat
     9601 accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc
     9661 gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac gcgccattag
     9721 cgccgtcccg acatctgccc ccagcacaat gaccagagcc ggtgcgaggg ctaccagatc
     9781 ctgagcgacg aacgaggtca ccagcatggt ggtggcatta ctgctctgta ccagtgcggt
     9841 aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt
     9901 acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag tcccccagac
     9961 cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag attctcctta
    10021 tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc tgactggcat
    10081 ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc tgtttccagt
    10141 ataagtgttt catattaata aagagacagg gcactcattg agcgccctgt ttgttgtaag
    10201 gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg ccagccagcg
    10261 ctcaacatcg gagacgctca tacctttacg gcgggcatgg tcttcaacct gatcgcgctg
    10321 aatttgcgcc accgcgtagt acttgctgtc cggatgactg aaataccaac ccgaaaccga
    10381 tgcaccaggc cacatcgcga aagattctgt gagtttcatg ccagtatgtt tttccacttc
    10441 cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccggat agcccggtgc
    10501 cggacggatg ccctggtagt tttcgcggat cagttcttcg ttgctgaggt tctcgttcgg
    10561 cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa aggcttcggc
    10621 taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct gcgcttcaaa
    10681 ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc cgatgtaatc
    10741 tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag cgaatcccgt
    10801 tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtgc gcgtttcgtc
    10861 acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca ccacgccacg
    10921 cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt ctttaaacag
    10981 ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgaggatact tcccggccag
    11041 cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagcgttt cgatgctggc
    11101 ttccacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct gccagtcaaa
    11161 tgcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgtg gtttcttgcg
    11221 cccgtgctga atacgcacgg tttcgtactc cttgcaggta cgagcgacaa aatcatcacg
    11281 ctgagtatcg gaaagcagcg ccgctaccac accaacggtg cgcgaggcat tctgcacata
    11341 caccgtaggg ccgctgtagt tctgctcgat tttcaccgcc gtgtgcgctt ttgaggtggt
    11401 cgcgccgcca atcagtaacg gaatggcgaa gccctgacgc tccatctctt tcgccacgtt
    11461 aaccatctcg tccagcgacg gcgtgataag ccccgaaagg ccaatcaaat cagcattcac
    11521 ttctttggcg atacggagaa ttttttccgc aggcaccata acgccgagat cgacaatttc
    11581 gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt ggacatcgcc
    11641 tttcacggtg gcgatcacca tcttgccgtt ggttttaccc tgctctttgc tggcttcaat
    11701 aaacggttcg agatacgcca ctgcctgttt catgacgcgc gccgatttga ccacctgtgg
    11761 caggaacatt ttgccttcgc caaacagatc gccgaccacg ttcatgccgt ccatcaacgg
    11821 gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcag tatcctgctc
    11881 gataaactcg gttatgcctt tgaccagcga gtattccaga cgtttattca cgtcccacga
    11941 gcgccactcc gcttgctggg cgttagcagt gtcgtccgct ttactgccgc gatatttctc
    12001 ggcaagctcc agtaaacgct cagtgccatc gtcacgacga ttaagaatca catcttccac
    12061 cgcgtcgcgc agttcagcgg gtaggtcgtc gtaaatcgcc agttgcccgg cgttgacgat
    12121 ccccatatcc atgccattgc gaatagcgta gtagaggaac actgcgtgaa tggcttcgcg
    12181 caccggatcg ttgccacgga atgagaaaga aacgttagat acgccgccgg aaatcagcgc
    12241 gtgcggcagt tcgcgtttga tgtcttcaca cgcgccgata aagtcctgcg cgtagttgtt
    12301 gtgctcttca ataccggtgg cgaccgcgaa gatgttcggg tcgaagatga tatcttctgg
    12361 cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct cgattttccg
    12421 tgcgcgagta tcggcctgtc cctgttcgtc aaaggccatc accaccactg ccgcaccgta
    12481 gcgacgcaac aatttcgcgt gatggataaa ggcgtcgacg ccctctttca tcgagataga
    12541 gttaacaatg cctttgccct ggatacactt cagacctttt tcaatgacat cccattttga
    12601 ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca gattgagaaa
    12661 acgcaccatc gccgcttcgg catcgagcat cccttcatcc atgttgatat cgataatctg
    12721 cgcgccgttt tccacctgct gacgcgccac atccagcgcc tcgctgtatt tctcttcttt
    12781 gatcaggcgc ttgaacttag cagagccggt gacgttggtg cgttcaccca cgttcacaaa
    12841 caggctatcg tcgccaatgt tcagcggctc caggccagac aaacggcagg ctacgggaat
    12901 ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg caatatgttg
    12961 cggcgtggtc ccgcagcaac caccgacgat attgagaaag cccgcttgcg cccattcacg
    13021 tatctgtttt gccatcgtgt cggcgtcgag atcgtattca ccaaaggcgt taggcagccc
    13081 ggcgttcggg tgggcggtga cgtagcattc cgcaatccgt gatagttcct gcacgtactg
    13141 gcgcagttca tcgggcccca gcgcacagtt caggccaaag gtcagcgctt cggcgtggcg
    13201 caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg aggcgtcggt
    13261 gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact ccgttttcac
    13321 cgcaaagacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca ggatcagatc
    13381 cgcaccacct tccaccagcg ctttggtgga ttcgcgatag gccgccgcca gctggtcaaa
    13441 agtgatgttg cggaatgccg gatcgttgac gtccggagaa atagacgccg tgcggttggt
    13501 tgggccaaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg tccactcgtc
    13561 agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca gggattccat
    13621 ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga tgatatccgc
    13681 gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttac tgagtaccag
    13741 caggtcgttg ttgcctttga ggtcgcatgg ccagtcggca aagcgttcac cacgaaaatc
    13801 ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca acaccagaat
    13861 acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg ctcccgacac
    13921 gcttaaccca gatttaataa aaattcaaca aaccatactg gaataaacgc atctgtggta
    13981 aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat cagttcagca
    14041 actattgcat tagctaacaa taaaaatgaa aataatttcc acgatacaga aaaaggaggc
    14101 tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg ccaccgcacc
    14161 agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt ggattgccga
    14221 atccaatggc agcgtggcac tcacagagct ggcgcaacaa gccgggttac ccaattccac
    14281 gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg tcggcgaact
    14341 gggacactgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc tccagagccg
    14401 taatttgtta gcaattgttc accctatcct gcgcaatcta atggaagagt ctggcgaaac
    14461 ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg accaggtaca
    14521 gtgtacccac ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga tgcacgcttc
    14581 cggtgcgggt aaagcctttt tagcccaact gagcgaagaa caggtgacga agctgctgca
    14641 ccgcaaaggg ttacatgcct atacccacgc aactctggtg tctcctgtgc atttaaaaga
    14701 agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac atgcgctggg
    14761 gctacgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg ccgcaatttc
    14821 tatttccgga ccgatttcac gtattaccga tgatcgcgtg accgagtttg gcgcgatggt
    14881 gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac tttttctggc
    14941 gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg gtgtctgtta
    15001 atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta tt
//
LOCUS       DAZPEU010000088        14959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPEU010000088 DAZPEU010000000
VERSION     DAZPEU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.089"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..256
                     /locus_tag="ACHU2L_002427"
     CDS             <1..256
                     /locus_tag="ACHU2L_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1357256.1"
                     /translation="KSLAKQLAPKGIRVNAVAPGPYWTVLQCCGGQPQEKIEKFGANA
                     PLGRPGQPVEIAPLYVTLAAEENSYTSGQVWCSDGGTGTL"
     gene            complement(352..996)
                     /locus_tag="ACHU2L_002428"
     CDS             complement(352..996)
                     /locus_tag="ACHU2L_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB4"
                     /protein_id="HFR1357257.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQ
                     FYDRESQKGCNFSRANLKDAIFKSCDLSMADFRNINALGIEIRHCRAQGSDFRGASFM
                     NMITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGE
                     FSSFDWRAANVTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLLERLGIAVMG"
     gene            complement(1079..2068)
                     /gene="pspF"
                     /locus_tag="ACHU2L_002429"
     CDS             complement(1079..2068)
                     /gene="pspF"
                     /locus_tag="ACHU2L_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1357258.1"
                     /translation="MVNFIMAGYKDNLLGEANSFIEVLEQVSRLAPLDKPVLVIGERG
                     TGKELIANRLHYLSTRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFE
                     RADGGTLFLDELATAPMLVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPRM
                     VSEGTFRADLLDRLAFDVVQLPPLRERQSDIMLMAEHFAIQMCREIGLPLFPGFTDEA
                     KETLLHYRWPGNIRELKNVVERSVYRHGTSDYPLNEIVIDPFRRHAAPPLAQETRHTS
                     VTLPLDLREFQQQQEKEFLQTSLQQAKFNQKKAAELLGLTYHQLRALLKKHQI"
     gene            2224..2892
                     /gene="pspA"
                     /locus_tag="ACHU2L_002430"
     CDS             2224..2892
                     /gene="pspA"
                     /locus_tag="ACHU2L_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1357259.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     SNSARALAEKKQLSRRIEQASAQQAEWQEKAELALRKEKEDLARSALIEKQKLTDLIA
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAASSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHNFGKQKSLDQQFADLKADDEISEQLAQLKAKMQ
                     QDKQ"
     gene            2947..3171
                     /gene="pspB"
                     /locus_tag="ACHU2L_002431"
     CDS             2947..3171
                     /gene="pspB"
                     /locus_tag="ACHU2L_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1357260.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSSRGELAQSEQQRLV
                     ELNQDAQRMRERIQALEDILDAEHPNWRDR"
     gene            3171..3530
                     /gene="pspC"
                     /locus_tag="ACHU2L_002432"
     CDS             3171..3530
                     /gene="pspC"
                     /locus_tag="ACHU2L_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1357261.1"
                     /translation="MGGVNLNKKLWRIPQQGMVKGVCAGIAHYLDVPVKLVRILVVLS
                     IFFGLAFFTFVAYIVLTFVLDPMPDNVVSGEQQPSSGELLDAVDRELAASEKRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            3539..3769
                     /gene="pspD"
                     /locus_tag="ACHU2L_002433"
     CDS             3539..3769
                     /gene="pspD"
                     /locus_tag="ACHU2L_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1357262.1"
                     /translation="MNNRWQRAGQRVKPGIKIAGKLVLLTALRYGPAGVAGWAVKSVA
                     RRPLKMLLALVLEPLLSRAATKLSKRYSGNQP"
     gene            complement(3958..4146)
                     /locus_tag="ACHU2L_002434"
                     /pseudo
     CDS             complement(3958..4146)
                     /locus_tag="ACHU2L_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
     gene            complement(4104..4925)
                     /locus_tag="ACHU2L_002435"
     CDS             complement(4104..4925)
                     /locus_tag="ACHU2L_002435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357263.1"
                     /translation="MDVLRFILRLPFILLRLAARSLVYLFTLLGFLLRPFTGRIRWAV
                     PGWVTFAGNQLARLERGGNRYPKTISALLLLTAAVAAGSYYTWHWYQNKPKPVDVAPL
                     VVQDISASVQRPSAVNYNRDDNSAQIVVVTFSRSAAPVTLIGKPVTAGITLTPAMEGE
                     WQWRNDRKLVFTAKKTFPMGKTYTVDMDAKTLLAPQVALTEKQKTFTTPEFYYRGGRA
                     EFYQDPQDPMKKHAIIGLTFNAPADVKNLESRLSMTRDGKPVPYTVTVMNCCHLC"
     gene            complement(5120..6259)
                     /locus_tag="ACHU2L_002436"
     CDS             complement(5120..6259)
                     /locus_tag="ACHU2L_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032097955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class C beta-lactamase DHA-1"
                     /protein_id="HFR1357264.1"
                     /translation="MKKSLSATLISALLAFSAPGFSAADNVAAVVDSTIKPLMAQQDI
                     PGMAVAVSVKGKPYYFNYGFADIQAKQPVTENTLFELGSVSKTFTGVLGAVSVAKKEM
                     ALNDPAAKYQPELALPQWKGITLLDLATYTAGGLPLQVPDAVKSRADLLNFYQQWQPS
                     RKPGDMRLYANSSIGLFGALTANAAGMPYEQLLTARILAPLGLSHTFITVPESAQSQY
                     AYGYKNKKPVRVSPGQLDAESYGVKSASKDMLRWAEMNMEPSRAGNADLEMAMYLAQT
                     RYYKTAAINQGLGWEMYDWPQQKDMIINGVTNEVALQPHPVTDNQVQPYNRASWVHKT
                     GATTGFGAYVAFIPEKQVAIVILANKNYPNTERVKAAQAILSALE"
     gene            6370..7245
                     /gene="ampR"
                     /locus_tag="ACHU2L_002437"
     CDS             6370..7245
                     /gene="ampR"
                     /locus_tag="ACHU2L_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator AmpR"
                     /protein_id="HFR1357265.1"
                     /translation="MVRRYLPLNPLRAFEAAARHLSFTRAAIELNVTHAAVSQQVRAL
                     EEQLGCVLFTRVSRGLVLTHEGEGLLPVLNEAFDRIADTLECFSHGQFRERVKVGAVG
                     TFAAGWLLPRLAGFYDSHPHIDLHISTHNNHVDPAAEGHDYTIRFGNGAWHESDAELI
                     FSAPHAPLCSPAIAEQLQQPDDVHRFTLLRSFRRDEWSRWLDCAGGTPPSPSQPVMVF
                     DTSLAMAEAAQLGAGVAIAPVCMFSRLLQSGALVQPFAAEITLGGYWLTRLQSRTETP
                     AMQQFARWLLNTAAA"
     gene            complement(7249..7614)
                     /gene="hypA"
                     /locus_tag="ACHU2L_002438"
     CDS             complement(7249..7614)
                     /gene="hypA"
                     /locus_tag="ACHU2L_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1357266.1"
                     /translation="MTDYDNGGRGKLSKALGAADIIREQAEQHGIARVTDVWLEVGAL
                     ADVEESALHFCFDIACRDTVAQGCTLHIDVIPAQAWCWDCSREAEIMQHAGCCPHCGS
                     ERLRISEGDDLRVKSLEGE"
     gene            7507..7842
                     /locus_tag="ACHU2L_002439"
     CDS             7507..7842
                     /locus_tag="ACHU2L_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HFR1357267.1"
                     /translation="MRYPRNAVLFSLFADNVRRTEGFTKLAPSAVVIIGYGIAFYFLS
                     LVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSPSW
                     KSLRRPTPW"
     gene            7836..8675
                     /gene="sul1"
                     /locus_tag="ACHU2L_002440"
     CDS             7836..8675
                     /gene="sul1"
                     /locus_tag="ACHU2L_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR1357268.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            8803..9006
                     /locus_tag="ACHU2L_002441"
     CDS             8803..9006
                     /locus_tag="ACHU2L_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357269.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            9162..10367
                     /gene="chrA"
                     /locus_tag="ACHU2L_002442"
     CDS             9162..10367
                     /gene="chrA"
                     /locus_tag="ACHU2L_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR1357270.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            10378..10683
                     /locus_tag="ACHU2L_002443"
     CDS             10378..10683
                     /locus_tag="ACHU2L_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR1357271.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            10910..11674
                     /locus_tag="ACHU2L_002444"
     CDS             10910..11674
                     /locus_tag="ACHU2L_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR1357272.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(12167..12751)
                     /gene="mphR(A)"
                     /locus_tag="ACHU2L_002445"
     CDS             complement(12167..12751)
                     /gene="mphR(A)"
                     /locus_tag="ACHU2L_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR1357273.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(12751..13989)
                     /gene="mrx(A)"
                     /locus_tag="ACHU2L_002446"
                     /gene_synonym="mrx"
     CDS             complement(12751..13989)
                     /gene="mrx(A)"
                     /locus_tag="ACHU2L_002446"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR1357274.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(13986..14891)
                     /locus_tag="ACHU2L_002447"
     CDS             complement(13986..14891)
                     /locus_tag="ACHU2L_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR1357275.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         3260 a         4178 c         4247 g         3274 t
ORIGIN      
        1 taaatcgctg gcgaagcaac tggcccctaa agggatccgt gtaaatgctg tcgcgcctgg
       61 gccgtactgg acggtattgc agtgctgcgg cggccaacca caggaaaaaa ttgagaaatt
      121 tggtgcaaat gcgccgcttg gtcgccctgg ccaaccggta gaaattgctc cgctgtatgt
      181 cactttagcg gctgaagaga acagctatac atccggtcag gtatggtgtt ctgacggggg
      241 gaccggaacc ctctgacgtt tatgcccccg aagtcgaaac gtctgagggg gaaagccagc
      301 ccggacaata ttgtataagg gcagggggcg gcatataccg ctccctgaac attaacccat
      361 gacagcgata ccaagacgtt ccaggagcaa cgatgcctgg tagctgtcca gtttgacgcc
      421 ttgcaaatca accccgcgga tatctaaatc gcccagttcc gaattggtca aatcacagtg
      481 cgtaacgttt gctgctcgcc agtcgaagga tgaaaactcg ccgccagaga ggtctgatcc
      541 actgaacgtt gcgcccagca cctgagtacc catccagcgg ttttcccaca gctcgcactt
      601 ttccagtacg acttttgaaa agttggcgta gcttaagttg gtattggtga tataggcgct
      661 acaaaaccag gtgcgggtgg tgatcatatt cataaaactt gcgccgcgaa aatctgaccc
      721 ttgtgcccgg cagtggcgaa tttcgattcc cagcgcattg atattcctga aatcagccat
      781 ggagagatca caacttttga aaatggcatc tttcaggtta gcgcgactaa aattacatcc
      841 tttctgactt tctcgatcat aaaactggca gccaataaat tcagtgccgc taaggtcggc
      901 acccgaaaaa tcacagttga aaaatgtgct attttcaact ttttcaccgg tgaacctgtt
      961 tctgtcaatt ttttcgccaa ctaacgccag agtcatcatg attaacctgt ttttttatac
     1021 agtgattgca acatggtaaa ctgttttaac tctcgcgtcc aacgtctgcc cctgcacgct
     1081 aaatttggtg tttcttcagt aatgcgcgga gttgatggta ggtcagacct aataattcag
     1141 cggctttttt ctgattaaat ttcgcctgct gtaagctggt ttgcaaaaat tccttctcct
     1201 gctgctgttg gaattcacgc agatccagcg gtagggttac tgatgtatgt ctggtttctt
     1261 gcgccagcgg cggtgcggcg tgacgcctga acgggtcaat cacaatctca ttcagtgggt
     1321 aatcgctggt cccgtgtcgg tataccgaac gctccacgac gtttttcagt tcacgaatat
     1381 tgcccggcca acggtagtgg aggagcgttt ctttggcctc atcggtgaac ccaggaaaca
     1441 acggtaaccc gatctcacgg cacatctgaa tggcaaaatg ttcagccatt aacatgatat
     1501 cgctttgacg ttcgcgtaac gggggaagtt gcaccacgtc aaaggccaga cgatccagca
     1561 ggtcggcgcg gaacgtgcct tcactcacca tgcgtggcag gtcggcgttg gttgcgcaaa
     1621 ccagtcttac attgacctgc agaggttggc taccgcctac gcgttccagc tctccgtact
     1681 caataacgcg cagcagtttt tcctgtacca gcatcggggc ggttgccagt tcatcaagaa
     1741 acagtgtgcc gccatcggcg cgctcgaacc gacctgggtg acgtttctgc gcgccggtaa
     1801 aggcgcccgc ttcgtgccca aagagttcgg aatcaagtaa attttcgttg agcgcggcgc
     1861 agttaagtga aataaacggg ccttgccaac gggttgagag gtagtgcagg cggttggcga
     1921 taagctcttt ccctgttcct cgttccccaa tgaccagcac aggtttatcc agcggcgcga
     1981 gacgagaaac ctgctccagc acttcaataa agctgttcgc ttcgcccagc agattatctt
     2041 tgtatcctgc catgatgaaa ttcaccattt gttagtgtta ttcaccaatt taccctattt
     2101 atcccgacag gtaaaattag ttattttcaa atatttcaat gaattaaaaa gttggcatgc
     2161 gaagtgcatt agaacagcag cagggcattg cccgatatca gaacagtaag tgaggattac
     2221 attatgggta ttttttctcg ttttgccgac atcgtgaacg ccaacattaa tgcgttgctg
     2281 gaaaaggctg aagatccgca gaagctggtg cggttgatga tccaggaaat ggaagacacg
     2341 ctggttgagg tgcgctccaa ctccgcacgc gcgctggcag aaaagaaaca gttatcgcgt
     2401 cgtattgaac aggccagcgc ccaacaggca gagtggcagg aaaaagcaga gctggcgctg
     2461 cgtaaagaaa aagaagattt ggcgcgttct gcgttaatcg aaaagcagaa actgactgac
     2521 ctgattgcct ccctggagca tgaggtgacg ctggttgacg atacgctggc gcgtatgaaa
     2581 aaagagatcg gtgaactgga aaacaaactc agtgaaaccc gtgctcgtca gcaggcattg
     2641 atgttgcgcc atcaggcggc gagttcttcc cgtgatgttc gtcgtcaact ggacagcggc
     2701 aaactggatg aagcgatggc tcgctttgaa tcttttgagc gtcgtatcga tcaaatggaa
     2761 gctgaagctg aaagccataa cttcggtaag cagaagtctt tggatcagca atttgctgac
     2821 ctgaaagcag atgatgaaat cagcgagcag ttggcgcaat tgaaagccaa aatgcagcaa
     2881 gacaagcaat aataatatct ggcggtgccc gaacgcgccg ccgctcatca cctgtaagga
     2941 gtactcatga gcgcgctatt tctggccatc ccgttaacca tctttgtgct gttcgtctta
     3001 ccgatttggc tctggctgca ttacagcaat cgttccagtc gcggcgagct ggcgcaaagt
     3061 gaacaacagc gtctggtaga acttaaccag gacgcacagc ggatgcgtga gcgcattcaa
     3121 gcgctggaag acatccttga tgctgaacat ccaaactgga gggaccgcta atggggggcg
     3181 ttaatctgaa taaaaaactg tggcgtatac cgcagcaggg catggtgaaa ggggtttgcg
     3241 cgggtattgc gcattatctc gatgttcctg tgaaactggt gcggatcctg gtggtgctgt
     3301 ccatcttttt tggtctggcc tttttcacct ttgtcgccta catcgtactg acgtttgtgt
     3361 tggatccgat gccagacaac gtggtttccg gagaacagca gccatcaagc ggtgagctat
     3421 tggatgctgt cgatcgtgaa ctggctgcaa gtgaaaagcg tttgcgtgag atggagcgtt
     3481 atgtaacgtc tgatactttt acattacgca gccgcttccg ccaattgtga ggttagaaat
     3541 gaataatcgc tggcaacgtg ccgggcaaag ggttaagccg ggcattaaaa tagcaggtaa
     3601 gctggtgcta ctgactgcgc tgcgctatgg cccggctggg gtagcagggt gggcggtgaa
     3661 gtccgtggcc cgacgtccgc taaaaatgtt gctggcgtta gtattggaac ctttgttgag
     3721 ccgggcagcg acaaaattgt cgaaacgcta ttcaggtaat caaccctgat atccgaaaca
     3781 acaaaagcaa gcaacattta gtcaacattc ccccagttaa tttgcggcag agtagtctct
     3841 tgtgtcatgg gatcgctacc aatagaaatg aaggtgaaag cgacagactc cattatgcag
     3901 gtatcggtgt gaattacgca ttctaaaatg cactggggca ttgttatgcc ccagttttta
     3961 taccagtgag tcggctaacg atatttgtgt acccacggtg atattttgta aacctttctc
     4021 accggcaatc aggctaaata ataaattaca gctttgttcg ccgagctgct gggttgggac
     4081 atcaatgcct cccggcacgg gcgtcagcat aaatgacaac agttcattac agtgacggta
     4141 tacggcaccg gcttgccgtc acgggtcata gacagacgcg attccagatt tttgacatca
     4201 gcgggggcat tgaaggtcag gccgataatg gcgtgttttt tcatcggatc ttgcggatcc
     4261 tgatagaact ccgcccggcc gccgcgataa taaaactcag gggtggtaaa ggttttttgt
     4321 ttctctgtta acgccacctg cggagccagc agtgttttgg catccatatc gacggtgtag
     4381 gttttcccca tcgggaaggt ttttttcgcc gtgaatacca gcttacggtc attacgccac
     4441 tgccactcac cttccattgc gggggtcagg gtaataccgg cggtgaccgg tttaccgatc
     4501 agcgttaccg gtgcggcaga gcgggagaat gtcaccacca caatctgcgc tgaattatca
     4561 tcacggttat aattaaccgc cgaagggcgc tgcacagacg cggaaatatc ctgcaccacc
     4621 agcggcgcaa catccaccgg cttcggtttg ttctgatacc agtgccaggt gtaataactg
     4681 cccgctgcca ccgcagcagt cagtaataat aaggcagata tggttttcgg atagcggttg
     4741 cccccccgct ccagccgggc aagctgatta ccggcaaagg tcacccagcc cggcaccgcc
     4801 cagcggatcc gtccggtgaa cgggcgcagc agaaaaccca gcagagtaaa gagataaaca
     4861 agactgcgag ccgccagacg cagcaaaata aaaggtaagc ggaggataaa acgcagaacg
     4921 tccatgacaa cattttcctc tgtgtgttga gactgacatc agtttgccgc agcacacagt
     4981 gaaatccggt tcagccggaa gctgtcagtg cccgatactc tcatcctcca taaaacagcc
     5041 cataaagcaa attattgcat aaggaataat cttaaattac ggccccggcg tatccgcagg
     5101 ggcctgttca ggaaaaaaat tattccagtg cactcaaaat agcctgtgca gctttgactc
     5161 tttcggtatt cgggtagttt ttattcgcca gaatcacaat cgccacctgt ttttccggaa
     5221 taaaggcgac ataggcgccg aaaccagttg ttgcgcccgt tttatgcacc caggaagcac
     5281 ggttatacgg ctgaacctgg ttgtctgtta ccggatgcgg ctgcaatgcg acctcgttgg
     5341 tcacaccgtt aatgatcata tctttctgct gcggccagtc atacatttcc cagcccagcc
     5401 cctggttaat cgcggcggtt ttatagtagc gggtctgggc gagatacatt gccatttcca
     5461 gatccgcatt accggcccgt gacggctcca tattcatttc cgcccagcgc agcatatctt
     5521 ttgaggcgga tttcacgccg taagattccg catcaagctg tcccggcgac acgcggaccg
     5581 gttttttgtt tttataaccg tacgcatact ggctttgcgc actttccggc acagtaataa
     5641 aggtgtgaga taaccccagc ggtgccagga tccgtgcagt cagcaactgc tcatacggca
     5701 tccccgccgc gtttgcggtc agagcaccaa acaggccgat actgctgttt gcatacagac
     5761 gcatatcgcc cggtttccgg gacggctgcc actgctgata gaaattcagc agatccgcac
     5821 ggctttttac cgcatccggc acctgtaacg gcagtccgcc tgcggtatag gtagccagat
     5881 ccagcaatgt gatccccttc cactgcggca gagccagctc cggctggtat tttgccgccg
     5941 gatcattcag cgccatctct tttttcgcca cagaaaccgc acccagcaca cctgtgaaag
     6001 ttttacttac agatccgagc tcaaatagtg tattttcagt gaccggctgt tttgcctgaa
     6061 tatcggcaaa accataattg aaataatagg gcttaccctt tacggagacg gcaaccgcca
     6121 tcccgggaat atcctgctgt gccatcagcg gtttaatggt gctgtccacc accgccgcga
     6181 cattatcagc ggcagaaaac cccggggcgg aaaacgccag cagagcggaa atcagtgttg
     6241 cagataacga ttttttcatc agaattaacc ttccgtgtgt gagtaacggc ggcaattatc
     6301 gtcagattca ccggacagaa caaacggtta tttataacaa gagcctaaag aaaaacttac
     6361 aggtggatta tggtcagacg ttatctcccc cttaacccgc tgcgcgcctt tgaggccgcc
     6421 gcccgtcatc tcagttttac ccgcgcggcg attgagctga atgtcaccca tgccgccgtc
     6481 agccagcagg tcagggcgct ggaagaacaa ctcggctgtg tgctgtttac ccgcgtctcg
     6541 cgcgggctgg tgctgaccca tgaaggtgag ggattactgc cggtgctcaa tgaggcgttt
     6601 gaccggattg cggatactct ggagtgtttt tctcacgggc agttccgtga gcgggtgaaa
     6661 gtcggtgcgg tgggaacatt tgccgcaggc tggctgctgc cgcgtctggc cggattctat
     6721 gacagccatc cgcatattga tctgcatatc tccacccata acaatcatgt ggacccggcg
     6781 gcggaagggc atgattatac gatccgtttc ggtaacggcg cgtggcatga gtcagatgcg
     6841 gaactgattt tcagtgcacc acacgctccg ctgtgctcac cggccattgc agaacagtta
     6901 cagcagccgg atgatgttca ccgctttacc ctgctgcgct cattccgccg ggatgaatgg
     6961 agccgctggc tggattgtgc gggcggcaca ccgccttccc cgtcacagcc ggtaatggtg
     7021 ttcgatacct cactggccat ggccgaggcg gcacaactgg gtgccggggt agcgatcgca
     7081 ccggtatgta tgttcagccg cctgttacag tcaggcgcac tggtacagcc gtttgccgca
     7141 gaaatcaccc tcggcggcta ctggctgacg cggttacagt cccgtacgga aaccccggcc
     7201 atgcagcaat tcgcccgctg gctgctgaat acggcggcgg cgtaaaactc actcaccttc
     7261 caggcttttt acccgcaaat catcaccttc actgatgcgc agccgttcac tgccgcagtg
     7321 cggacagcat ccggcgtgct gcatgatttc ggcctcacgg ctgcaatccc agcaccatgc
     7381 ctgtgccggg ataacatcaa tatgcagtgt gcagccctgc gccacggtat cacggcaggc
     7441 gatatcaaaa cagaaatgca gtgcactctc ctcaacatcc gccagtgcgc cgacttccag
     7501 ccagacatcc gttacccgcg caatgccgtg ctgttcagcc tgttcgcgga taatgtccgc
     7561 cgcaccgagg gctttactaa gcttgcccct tccgccgttg tcataatcgg ttatggcatc
     7621 gcattttatt ttctttctct ggttctgaaa tccatccctg tcggtgttgc ttatgcagtc
     7681 tggtcgggac tcggcgtcgt cataattaca gccattgcct ggttgcttca tgggcaaaag
     7741 cttgatgcgt ggggctttgt aggtatgggg ctcataattg ctgccttttt gctcgcccga
     7801 tccccatcgt ggaagtcgct gcggaggccg acgccatggt gacggtgttc ggcattctga
     7861 atctcaccga ggactccttc ttcgatgaga gccggcggct agaccccgcc ggcgctgtca
     7921 ccgcggcgat cgaaatgctg cgagtcggat cagacgtcgt ggatgtcgga ccggccgcca
     7981 gccatccgga cgcgaggcct gtatcgccgg ccgatgagat cagacgtatt gcgccgctct
     8041 tagacgccct gtccgatcag atgcaccgtg tttcaatcga cagcttccaa ccggaaaccc
     8101 agcgctatgc gctcaagcgc ggcgtgggct acctgaacga tatccaagga tttcctgacc
     8161 ctgcgctcta tcccgatatt gctgaggcgg actgcaggct ggtggttatg cactcagcgc
     8221 agcgggatgg catcgccacc cgcaccggtc accttcgacc cgaagacgcg ctcgacgaga
     8281 ttgtgcggtt cttcgaggcg cgggtttccg ccttgcgacg gagcggggtc gctgccgacc
     8341 ggctcatcct cgatccgggg atgggatttt tcttgagccc cgcaccggaa acatcgctgc
     8401 acgtgctgtc gaaccttcaa aagctgaagt cggcgttggg gcttccgcta ttggtctcgg
     8461 tgtcgcggaa atccttcttg ggcgccaccg ttggccttcc tgtaaaggat ctgggtccag
     8521 cgagccttgc ggcggaactt cacgcgatcg gcaatggcgc tgactacgtc cgcacccacg
     8581 cgcctggaga tctgcgaagc gcaatcacct tctcggaaac cctcgcgaaa tttcgcagtc
     8641 gcgacgccag agaccgaggg ttagatcatg cctagcattc accttccggc cgcccgctag
     8701 cggaccctgg tcaggttccg cgaaggtggg cgcagacatg ctgggctcgt caggatcaaa
     8761 ctgcactatg aggcggcggt tcataccgcg ccaggggagc gaatggacag cgaggagcct
     8821 ccgaacgttc gggtcgcctg ctcgggtgat atcgacgagg ttgtgcggct gatgcacgac
     8881 gctgcggcgt ggatgtccgc caagggaacg cccgcctggg acgtcgcgcg gatcgaccgg
     8941 acattcgcgg agaccttcgt cctgagatcc gagctcctag ggatcgcctc agaaaacgga
     9001 aaataaagca cgctaagccg taagtaagcg tgctcctgtg aaagccacag ctaaaactgc
     9061 gtagtacaca tagaggtatt tttttctaga tttatatcga atttcgttat cgaatttcgc
     9121 ttattattga tcaactcgtt gcagatcatg aaggcgaaag tatgacgaat aaccctaccg
     9181 atgacagcag accatggtcg gtctttctta tttttctgcg gcttggattg acatcttttg
     9241 gcggccccat tgcgcacttg ggctacttcc gcgccgaatt tgtcacacgg cggcgctggc
     9301 tctccgaacg gagctatgct gacttggtcg cgctttgtca gttcttgcca gggcctgcaa
     9361 gcagccaggt cggcatagcg gtaggactgt ctcgggctgg atacagcggg gcgctggctg
     9421 cttgggctgg cttcacgctg ccgtctgcca tagccttgat cctttttgcg ctcggcatct
     9481 ccagctatgg cgattacgtc tcgcagggcg cgttgcatgg cttaaaagtg gtggctgtgg
     9541 ccgtggtcgc tcaagcagta tggggcatgg cgcgtaacct atgcacggat gggctgcgag
     9601 tcaccatcat ggcaattgct acctgcgtcg ttttacttgt gccgtccgcg tggggacagg
     9661 ttggcgtgat tgctatcgca ggcatcgcag gccggttatt gttcaagcca gcgaaagttg
     9721 ttgagcatga ccccctacct atcacggtca gtcaccgggc cggcgtgctt tggctctcgc
     9781 tgttctttgt cttgctgatt ggcctgccgg tgttggccga actgatgcca agtcaaacca
     9841 tggcaatggt ggattccttc tatcgtgtcg gatcactggt gttcggcggt ggtcacgttg
     9901 tgctgccatt actgcaagcc gaagtggtgc cctccggctg ggtcaacaat gaatcctttc
     9961 tcgcggggta cggggcagct caagcggtgc ccggcccttt gttcacgttc gccgcgtttc
    10021 ttggtgcctc gatgaacacc gccccgtcgg gctggatcgg cggcattgtg tgtctgctgg
    10081 ctatcttcgc gccctcgttc ttgctggtcg tcggatcaat gccattttgg gagcgtttgc
    10141 gccgcaatac aggcatccaa gctgcgctgg ccgggatcaa tgccgctgta gtcggcttgc
    10201 tgctggccgc gctgtatcag cctgtatgga ctagcgccat ctttcagccg caagacttcg
    10261 gcttggcatt agttgccctt gtcgcactta tgttctggaa gctcccgccg tggctggttg
    10321 tcctcggtag cggtgcagct gggtggctgt tgagcgtcgc tctgtgaggc acaaaaaatg
    10381 actgacaaag acctctacgg cggtttgatc cgcctgcaca tccttcacca tgcagccgag
    10441 gaacctgtct ttgggctggg gatcatcgaa gagctacgcc gacacggcta cgagatgagc
    10501 gctggcaccg tgtacccgat gctgcacggc ctggaaaaaa aaggctatct gacctcacgc
    10561 cacgaacgca ccgggcgacg tgaacggcgt gtttatgaca tcactgaaca aggcagaact
    10621 gcacttgctg atgcgaaaac aaaggtcaag gagctgttcg gagagttggt agaaggtggt
    10681 tgagtttttg cttctatgtc aggttgagct ataccctaac tggattgtca ttttcagaag
    10741 acgactgcac cagttgattg ggcgtaatgg ctgttgtgca gccagctcct gacagttcaa
    10801 tatcagaagt gatctgcacc aatctcgact atgctcaata ctcgtgtggg ctctgttgca
    10861 aaaatcgtga agcttgagca tgcttggcgg agattggacg gacggaacga tgacggattt
    10921 caagtggcgc catttccagg gtgatgtgat cctgtgggcg gtgcgctggt attgtcgcta
    10981 tccgatcagc tatcgcgacc ttgaggaaat gctggcggaa cgcggcattt cggtcgacca
    11041 tacgacgatc tatcgctggg tccagtgcta cgccccggag atggagaagc ggctgcgctg
    11101 gttctggcgg cgtggctttg atccgagctg gcgcctggat gaaacctacg tcaaggtgcg
    11161 gggcaagtgg acctacctgt accgggcagt cgacaagcgg ggcgacacga tcgatttcta
    11221 cctgtcgccg acccgcagcg ccaaggcagc gaagcggttc ctgggcaagg ccctgcgagg
    11281 cctgaagcac tgggaaaagc ctgccacgct caataccgac aaagcgccga gctatggtgc
    11341 agcgatcacc gaattgaagc gcgaaggaaa gctggaccgg gagacggccc accggcaggt
    11401 gaagtatctc aataacgtga tcgaggccga tcacggaaag ctcaagatac tgatcaagcc
    11461 ggtgcgcggt ttcaaatcga tccccacggc ctatgccacg atcaagggat tcgaagtcat
    11521 gcgagccctg cgcaaaggac aggctcgccc ctggtgcctg cagcccggca tcaggggcga
    11581 ggtgcgcctt gtggagagag cttttggcat tgggccctcg gcgctgacgg aggccatggg
    11641 catgctcaac caccatttcg cagcagccgc ctgatcggcg cagagcgaca gcctacctct
    11701 gactgccgcc aatctttgca acagagcccg ccgtgctagt ctgctcggtg atggtggagt
    11761 gaagccaacc cgcaatcggg ttatgaatct gcatcgcgat tcgcaatcag ctgtctcttg
    11821 agcatgtcga actcctgcga tgttatctcg ccgtgctcgt gctgctacac caacttgtcc
    11881 aactcatccg ccacaccgct ccccaccacc tgcgatggcc aggttgtttg ctgggcgcat
    11941 tggcggagta tcgtctcgac ccgggacacc cacaccggca caaccttacg ggagtgattc
    12001 actgtcaaag aatcggcccg gtgctctgac gcaagtatcg ggatggtcac catttgtaag
    12061 ccgtagacct gagtggtgat caagacttcg ataccaccga ccgtaccggt actaatcgac
    12121 gacggtcgtg ttcgtcgcct gccgcaggga ctctgcacac ctccgtttac gcatgtgcct
    12181 ggaggagttg gaaatcgtcg tgttcgggaa acattaaaca caggatggca gcgatctgag
    12241 ccagcacatg atcagctagc tcaccatccg gatcgacggc ccactgcatc gtcgcgccag
    12301 cgatgaccga gtgcaggagc aactcagctg ccgcaggagc acctgggggc agtcgcttgc
    12361 ggatcccctc caccaccgcg cggttccgct ggatcgcaag cgtgcgtagc tccggcacct
    12421 ggagctcgta ccaggagatg agatagttca ccgagaagtc gttgcgagtg ttcatgctcc
    12481 gaacgagcac ctgcaaaaat tcccagagcc cttgcggccc tgcgcctatc ggtatcgcat
    12541 tcaggtaatg ccgcacctgc tcgacgccgc gctccatcat cctcaccagc agcgtatcgc
    12601 ggttggtgaa gcgctggatt aacgctgcgc gggagagccc cacctccttt gctactccgc
    12661 tgagcgtgaa ctctatggga ccgcaacgct tcagcactac ggtggcggcc tcgagtacct
    12721 cgtcatcgga cttgagcttg gggcggggca tcagtgttca ccttctgtat gggttggggc
    12781 ggaggctgtg gctgccgccg ccattgtagc aaattgaaga cggagcgaga gtagagccac
    12841 gagccccgca aacacggccg atacaacgag gccagggagc ggaccagcaa ggtcgacaaa
    12901 cgcgccggcc gcaagcataa ccatgggcga ggctgacagc atcaccgccg agaccgtgcc
    12961 gagtacccgg ccgagaagtt ctggcggcgt gcggttgtag atggcagcgt tgagaatggg
    13021 agagactgag ccggtcagca gtcccacgag cgcgcccaac aacatcagca ccggcacgcc
    13081 tggcaactgt gaaagcagaa gcgagcccac cgcagagcca caaaatgcca ccgccagcca
    13141 gttctgcgct gatatccggg cgccgaccga cgcatgaatg gcaatgccaa ggagaccacc
    13201 agcccccatc attgaggaga acagcccgag ctctgctact tggcgtcctg catctacaaa
    13261 cagcgcaggc atgatgacgc tgccgttggc gccaacgatg cccacgaaga tcatcactat
    13321 accaaagaga gggcgcagca ggggttcgct ccagagaaaa gcgacgccgg cgcgcatgga
    13381 gagagtcgcc gtcgtggtca tcgtccgagc ggcacgcgcg ggaagcaccc acgcgccgag
    13441 cagacctgca aggacggagc agaacgccgt cagcccgagc gttggcgcag cgccaagcag
    13501 gccgattgcg gcccccccaa gggccgggcc acctagaatc gcgacgttcc cgatcaccgc
    13561 tttcagtgac gagacgcgct caacggagag cccggcgacg tggccgagtt tgggcagctc
    13621 actgtcctgc gcggccatac cgggtgcgtc gaacgcggca ccgagcacca cgcaagcgat
    13681 cagcccagtg ttcgagaggg cgccaacggc atcgagcagt gggatgctcg ccatggccac
    13741 gccgcccacc acacccgaga tcaatgcgac gggcgcgcgc ccgaaccgat cgacgaggcc
    13801 accaccaacc cacgcgccga tgatggtcgc gatgacgctg ctagcggccg tggcgcccgc
    13861 ccaggccgcg ctctttgtat gagacaggac gaaccatgga agcgcgaggg ccgccaccgc
    13921 gttgccgatc cggaagagaa aggtcgccgc gaacagcgtc gcgagcgggc tatatcgacg
    13981 ttcgctcatt ccgctgcggc gagctgcgcc ttcgccgcag cgaggtactc ttcgttaccc
    14041 gagtcgaggg cgaagagtgc gtaggtgacc gccccgaacg caaggcgctc cgcgatgtgg
    14101 tgggcgagcc gcggccacac ccggccaccg gccgcttcat acgtgaggag gagcttcgcg
    14161 agcccctctt caccaaagac cataaggtgc gcggccatgt cgatggcagg gtcatcaacg
    14221 cgggcctcgc tccagtcgat catcccgctg acgcgctccg tgttgtcgat gagcacatgg
    14281 cccacgtaga gatcgccatg caccaccacg gagaaatctg gccacgacga atcgtcgtcg
    14341 agccagcgct gccaccggtg gaggcgcttg tcgttcacca cgaactcgcg tcggacgcgg
    14401 tcaacgtcgt cggccacctt ctgacgggcc tgcgtcggtg tacggatgag catccccgca
    14461 tccacggcgg cggaaatggg gacggcatgc agggcggcga gcgcggtcgc gaagctctcc
    14521 gcgaagacct ccgagtcctg cggcacgacc cagtcgggcg tggacgaacc aggctggatg
    14581 accatcgcag tcgagtcttc gagcatggga taggcaacga gctcggcgtt ggccacgcgc
    14641 cagtccggca ccgcgaacgg caggcgattc ttgagcattg ccagcacccg cgcctctggt
    14701 tcgaccttcg cgcttacctc ggctcggcgc gggatgcgca gcacccaccg acgtccatcg
    14761 tcgacggtgg cgatcacgat cctatagtcg agcccaagct cattgacagt cagcgggcca
    14821 tggagcttga gcccatgtcg ggctgcaagt gcgtacagtt gggaggtatc ggcggtcgtg
    14881 actacggtca tgattcactc ctgagggctt gacgggttta gccacctaaa tgtaacagtc
    14941 acgtcggtta tattcaatc
//
LOCUS       DAZPEU010000089        14939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPEU010000089 DAZPEU010000000
VERSION     DAZPEU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.090"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            65..307
                     /gene="yjbE"
                     /locus_tag="ACHU2L_002448"
     CDS             65..307
                     /gene="yjbE"
                     /locus_tag="ACHU2L_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR1357276.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            421..1059
                     /locus_tag="ACHU2L_002449"
     CDS             421..1059
                     /locus_tag="ACHU2L_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR1357277.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1056..1793
                     /locus_tag="ACHU2L_002450"
     CDS             1056..1793
                     /locus_tag="ACHU2L_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1357278.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1793..3889
                     /locus_tag="ACHU2L_002451"
     CDS             1793..3889
                     /locus_tag="ACHU2L_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1357279.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3936..4214)
                     /locus_tag="ACHU2L_002452"
     CDS             complement(3936..4214)
                     /locus_tag="ACHU2L_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357280.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4215..4358
                     /locus_tag="ACHU2L_002453"
     CDS             4215..4358
                     /locus_tag="ACHU2L_002453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357281.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4484..4894
                     /gene="psiE"
                     /locus_tag="ACHU2L_002454"
     CDS             4484..4894
                     /gene="psiE"
                     /locus_tag="ACHU2L_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR1357282.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4938..6413)
                     /gene="xylE"
                     /locus_tag="ACHU2L_002455"
     CDS             complement(4938..6413)
                     /gene="xylE"
                     /locus_tag="ACHU2L_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR1357283.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6784..7674)
                     /gene="malG"
                     /locus_tag="ACHU2L_002456"
     CDS             complement(6784..7674)
                     /gene="malG"
                     /locus_tag="ACHU2L_002456"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR1357284.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7689..9233)
                     /gene="malF"
                     /locus_tag="ACHU2L_002457"
     CDS             complement(7689..9233)
                     /gene="malF"
                     /locus_tag="ACHU2L_002457"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR1357285.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9387..10577)
                     /gene="malE"
                     /locus_tag="ACHU2L_002458"
     CDS             complement(9387..10577)
                     /gene="malE"
                     /locus_tag="ACHU2L_002458"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR1357286.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10942..12057
                     /gene="malK"
                     /locus_tag="ACHU2L_002459"
     CDS             10942..12057
                     /gene="malK"
                     /locus_tag="ACHU2L_002459"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR1357287.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12129..13469
                     /gene="lamB"
                     /locus_tag="ACHU2L_002460"
     CDS             12129..13469
                     /gene="lamB"
                     /locus_tag="ACHU2L_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR1357288.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13621..14541
                     /gene="malM"
                     /locus_tag="ACHU2L_002461"
     CDS             13621..14541
                     /gene="malM"
                     /locus_tag="ACHU2L_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR1357289.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14770..>14939
                     /locus_tag="ACHU2L_002462"
     CDS             14770..>14939
                     /locus_tag="ACHU2L_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357290.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDY
                     FIYISDIFLNIK"
BASE COUNT         3717 a         3827 c         3913 g         3482 t
ORIGIN      
        1 atactcgatc ccgcccgaaa tgtttttggg taaatctcca ttcattcaat gaagggaaat
       61 tgttatgaaa aaagttctgt atggcatttt tgccatatct gcgcttgcgg cgacttctgc
      121 gtgggctgca cctgtacagg tgggcgaagc ggcagagtcg gcagcaacgt cggtttcggc
      181 ggggagttcc tccgcgacca gcgtcagcac cgtaagctcg gcggtgggtg tcgcgctcgc
      241 ggcaaccggt ggcggtgatg gttctaatac cgggaccacc acaaccacga ccaccagtac
      301 ccagtaataa agtatgtatc cccaaaataa tttgagtcat tgcatctgtg gcttgaagta
      361 tgaagggatt taccataacc acactccggt gtggttattc tgcccctctg gagaagagtc
      421 gtgaagcgac ctgcactcat tcttatctgc ctgctattac aggcctgttc agccacgact
      481 aaagagctgg gcaattcact gtgggacagt ctgttcggca cgccaggcgt acagctgacg
      541 gacgatgata ttcaaaatat gccctacgcc agccagtaca tgcagcttaa tggcgggccg
      601 caattgtttg tggtgctggc cttcgctgaa gacggacaac aaaaatgggt cactcaggat
      661 caagccactc ttgtcacaca acatggtcgt ctggtgaaga ctttgcttgg cggcgacaac
      721 ctgattgaag tgaataacct tgccgccgac ccgctgatta aacccgcaca aattgttgat
      781 ggtgcaacat ggacgcgcac gatgggctgg accgagtacc agcaggtacg ctacgccacc
      841 gcacgctcag tcttcaaatg ggatggcacc gataccgtca aggtcggcag cgatgaaacc
      901 ctggttcgcg tgctggacga agaagtctcc accgaccagg cgcgctggca taaccgctac
      961 tggatcgaca gcgaagggca aattcgccag tcggaacagt atctcggcgc ggattatttc
     1021 ccggtgaaaa ccactctcat caaggcggca aaacaatgat taaacaaact attgtcgcgt
     1081 tgcttttgag tgtgggagcg tcatcggtct tcgcggcagg aaccgtcaag gtgttcagca
     1141 atggcagcag tgaggccaaa acgctaacgg gcgcagagca tttaatcgat ctggtagggc
     1201 aacctcgact ggcaaacagc tggtggcctg gtgcggtgat tagcgaagag ctggcaacgg
     1261 cggcagcatt gcgtcagcag caggcgttgc tgacacggct ggcagaacag ggcgcagatt
     1321 ccagcaccga cgatgccgcc gccattaacg ccttacgcca gcaaatccag gcattgaaag
     1381 tgacaggcag gcaaaaaatc aatcttgatc ccgatatcgt ccgcgttgcc gaacgcggta
     1441 acccgccgtt gcagggcaac tacacgctgt gggtcggacc accgccgtcc acggtcacgt
     1501 tgttcgggct tatcagccat cctggcaatc agccattcac tccaggtcgc gacgtggcga
     1561 gctatctctc tgaccagagc ctgctcagcg gtgcggatcg cagctacgcg tgggtcgttt
     1621 acccggacgg acgcacgcaa aaagcgccgg tggcttactg gaacaagcgt cacgtagagc
     1681 cgatgcccgg cagcattatt tatgttggcc tcgcagattc cgtctggagt gagacgcctg
     1741 atgcccttaa cgccgacatt cttcagactc tgacgcagcg gataccccaa taatgaaaaa
     1801 aagacatctg cttagcttac tggcgctggg cattagcaca gcttgctacg gcgaaacata
     1861 tcctgcgccc attggtccgt cgcagtcgga tttcggtggc gtaggattat tacaaactcc
     1921 caccgcgcgt atggcacggg aaggggagtt gagtctgaac tatcgcgata acgatcagta
     1981 ccgttattac tcagcttcag tgcaactctt cccgtggctg gaaacaacgc tgcgctacac
     2041 cgacgtgcgc acccggcagt acagcagcgt cgaagcgttc tctggcgatc aaacgtataa
     2101 agataaagcc ttcgatctca aactgcgttt gtgggaagag agttactggc tgccgcaagt
     2161 ggcggttggt gcgcgggata tcggcggtac ggggctgttt gatgcggaat atcttgttgc
     2221 cagcaaagcc tgggggccgt tcgattttac gctcggcctg ggctggggat atctgggcac
     2281 cagcggtaat gtaaaaaatc cgctctgttc agccagtgat aaatattgct atcgcgataa
     2341 cagctacaaa caggcgggat ctatcgacgg tagccagatg ttccacggtc ctgcctcact
     2401 gtttggcggc gtggaatacc agacgccctg gcaaccgctg cgtctgaaac tggagtatga
     2461 aggtaataat tatcagcagg attttgccgg gaagctggag caaaaaagta agtttaacgt
     2521 cggggcgatt tatcgcgtta ccgattgggc cgacgttaac cttagctatg aacgtggcaa
     2581 cacctttatg tttggcgtca cgctgcgcac caactttaac gatctgcgcc cgtcctacat
     2641 cgataacgcc cgcccgcaat atcaaccgca gccgcaggat gccattttgc agcattcggt
     2701 ggtggcgaat cagttaacgt tgctgaaata caacgccgga cttgccgatc cgcagatcca
     2761 ggcgaaaggc gatacgctgt atgttaccgg cgagcaggtg aaatatcgtg attcgcgcga
     2821 agggatcatc cgtgctaatc ggatcgtgat gaacgatctg ccggatggga tcaaaacgat
     2881 ccgcattacg gaaaatcgcc ttaacatgcc gcaagtgacg acggaaaccg atgtcgccag
     2941 cctgaaaaat catctcgccg gagagccgtt gggccacgaa acgacgctgg cgcacaaacg
     3001 cgtcgagcca gtggttccga agtccaccga gcagggctgg tatatcgaca aatcacgctt
     3061 tgatttccat atcgatccgg tgctgaacca gtcggtcggt ggcccggaaa acttttacat
     3121 gtatcagctg ggcgtgatgg gaacggcaga tttgtggctg acggaccatc tactgaccac
     3181 cggcagcctg tttgcaaatc ttgccaacaa ctacgacaag tttaactaca ccaatccacc
     3241 gcaggactcg cacttaccgc gcgttcgtac ccatgtgcgc gagtatgtgc agaacgatgt
     3301 ctatgtgaat aacctgcaag ccaactactt ccagcatctg ggcaacggct tctacggtca
     3361 ggtctatgga ggttatctcg aaaccatgtt tggcggtgcg ggggccgaag tgttgtatcg
     3421 tccgctggac agtaactggg cgtttggtct ggatgccaac tacgttaaac agcgcgactg
     3481 gcgtagtgca aaagatatga tgaaattcac cgactacagc gtgaaaaccg gacatctgac
     3541 cgcctactgg acgccatctt tcgcccagga cgtgttggtg aaagccagcg tcgggcagta
     3601 tctggcaggg gataaaggcg gcacgctgga gatcgctaaa cgctttgaca gcggcgtggt
     3661 ggtgggtggc tatgccacga tcaccaacgt ttcgaaagag gagtacggcg aaggggactt
     3721 caccaaaggc gtgtatgtct cggtaccgtt ggatctcttc tcgtctggcc cgacacgcag
     3781 ccgtgcggcg attggctgga cgccgctgac gcgtgacggt ggtcagcaac ttgggcgtaa
     3841 gttccagttg tatgacatga ccagcgaccg tagcgtaaat ttccgctaag tcatgggaaa
     3901 ggtgccagtt ttcgcactca ctggcaccct cattcttaat gacctgttga gccatcagga
     3961 acgcagttca cccccggttt acgatctggt gtgtaaaggc gaaaacattc tgtcttcctg
     4021 tccgtcgata tcggttgcag gtctggttgc ctgagcgact gggccatcat tgggctggag
     4081 acattgcttt gcggtaggtc ggctttatca gcagtaccga gcggaccagc ataagcagga
     4141 agaacagaga ctgataacat caaagcagca aaataaggct tcatttttac cacctttatc
     4201 aggttacgtt tcatttgttc cagaggaaca ttgtcgattt ttcgcgcatt gctggtggct
     4261 gggaatcacc tgaatgggtg atttttgaat taccggcttt ggtgcggttt gtcttgccgg
     4321 atgcgccgcc aggcgcggct tatccggcct acgggtaggt atatccggct ttggtggagg
     4381 cgcgctccaa atccaggttg aacaaaacat acacaaaaaa tatagatctc cgtcacattt
     4441 ttgcgttata caggaagctc gccactgtga aggaggtact gctatgacgt cactctctcg
     4501 tccgcgcgtg gagtttatct ccaccatttt gcagaccgta ctcaatcttg gcctgctgtg
     4561 cctcggcctg attttggttg tcttcctcgg caaagaaacg gtgcatctgg ctgatgtgct
     4621 gttcgcgcca gaacaaacca gcaaatatga gctggtagaa ggactggtgg tttactttct
     4681 ctatttcgaa tttatcgcgc tgattgtgaa gtactttcag tccggttttc acttcccgct
     4741 acgttacttt gtctatattg ggatcaccgc cattgtgcgc ttgatcatcg tcgatcataa
     4801 atcgccactg gatgtgttga tctactcggc tgcgatcctg ttactggtga tcaccctgtg
     4861 gctgtgtaat tcgaaacggc tgaagcggga gtaaaaagac agcacgccga aatggcgcgg
     4921 cgtgctggac aggaagatta cagcgtagca gtttgttgtg ttttcttcgt ttccggttcc
     4981 cagagcgctt ccagctcctc aagggtttta cctttggttt ccgggacaaa tttccacata
     5041 aacagtgctg ccagaacgcc catacaaccg taaatccagt aggagaaacc gttgtggaaa
     5101 tgggccacca gccaggagtt tttgtccatc atcgggaagg tccaggagac gaagtagttc
     5161 gccagccact gggccgccac cgcgattgcc agcgctttac cacgaatagc attcgggaag
     5221 atttccgaca gcagtaccca gcataccgga ccccaggaca tggcaaaggc ggcaacatag
     5281 aacagcatcg acagtagcgc cacaataccc gatgcctgag tgtaaaacgc ggtaccgagg
     5341 ctaaacatac cgattgccat tccgagtgcg ccgataattt gcagtggctt acgaccaaat
     5401 ttatccacag tcataattgc cagtacggta aaggtgaggt tgataactcc gacaataatg
     5461 gtctgcaaca gcgcgatatc cgtgctggcc cccagcgttt tgaacacttc cggcgcgtag
     5521 tacagcacca cattgatgcc gacaaattgc tggaagatgg agagcataac gccgattaca
     5581 atcacgccca cgccaaacat cagcagacga ccaccggttt tgcggccatg atccagggag
     5641 tgtttaattt cctgtactgc ctgagttgca agcgtgttgc ccataatttt gcgcaggata
     5701 ctttccgtct gttcttgctt gccgcgtgac atcagccagc gaggactttc tggcacggta
     5761 tacagcagca ttaagaacag cagtgcaggg atacattccg aggcaaacat ataacgcctg
     5821 ccgtcagtat tcagccagct ggcatcaccg gaacgggcaa taaaatagtt tacgcagtaa
     5881 actaaaagtt gcccgaaaat aatcgcaaac tggttaaatg aaaccagttt tccgcgaata
     5941 tgagctggag ccagttccgc aatatacatt ggtgaaagca ttgaggctaa accaacgcca
     6001 ataccgccaa taatacgata aataacaaat tccgggacat aacctgccag ataaataggt
     6061 actgtgttgt ccgggtttat agaggtaaaa ccaagttctg gccaggcaga acctacacca
     6121 gaaataaaaa acaggacagc agaaatctta agtgaatcac gacggccgaa gcggttactg
     6181 caataaccac cgagggcacc gccgatgatg caaccaatca gagcgctggc tacgcaaaat
     6241 cctaacaggg agttggcagc ggattcactt aagttttgtg gagcaacaaa gacggtattg
     6301 agtgactcaa cagtaccgga aataacggcg gtgtcgtagc caaataataa accacctaat
     6361 gtagcgacta aggtaatcga aaatatataa ctggaattat actgggtatt cattcagacc
     6421 tgccttagac cattctgatc ttttatcagt gcattgatgt tggaattgtc aggcagttaa
     6481 ggcaattatc attttttaca atgcgtttac gtaatgtctt ttctgtgatc ttaattgtga
     6541 taattatcaa aaattgatga aacaatacta ttgccgtgac tcagagcacg aaagagaatt
     6601 atcgtaagtg ggaaaacaaa taacgtaaaa ataataagtt ctatgatggg aatattaacc
     6661 ggcgaacgat tcagattgca gacgaaagaa aaaaaggcgc tccgtggagc gccgaataat
     6721 agtcacaagt tgggataacg taagttgagg gtgcagcggc ataacattgg cagaacaaca
     6781 tctttaacct ttcacaccac ctgccgtcag gccgttgacc agccagcgtt gagccagcaa
     6841 gaagacgatg gtgatcggta atgcagacat cacggcagcg gcggcaaagt caccccacag
     6901 gtagttttgc gggttgaggt attgctgcat ccccacggcc agggtgtagc tgtttacgtc
     6961 acgcagtaac agcgacgcga ccggaacttc agtaatggca gcgataaacg acaggatgaa
     7021 taccaccgcc agaatcggta ctgacagcgg caacaggaca aggcggaagg cctgccacgg
     7081 tgtcgcacca tccagcgcag ccgcttcttc cagcgaactg tcgatggttt cgaaatagcc
     7141 tttgatggtc cagacatgca gcgcaatccc acccagatac gcgaaaatta cgccgccgtg
     7201 agtattcagg ccaatgaatg gaatgtactc acccagacga tcaaacaacg catacaacgc
     7261 gaccagtgaa agtactgccg ggaacatctg gaaaatcagc attcctttca gcagcgtcgc
     7321 tttgcctgga aagcgcatac gggcgaaagc gtaggcgcag gtggtggaga gcgccacaat
     7381 gccaatcgcg gaaatcccag cgacctttac cgagttccac agccacagca gtaccgggaa
     7441 tggcggtggc gtaatgcgac catcagcctg ttcaacgcta aaacctaacg ccagtttcca
     7501 gtgatcccag gagatttgct ccgggatcag gctaccggtc gcgaagtttc cctgacgcag
     7561 cgagatagcg acgaccatca gcagcgggaa cataatcgcc gcgataaaaa gtagcagtag
     7621 caggtgagta ataaacaaac gtgctttttg cgatttcggt tggaccattg ccatttttgt
     7681 tatctccctt aatcaaactt cattcgcgtg gctttcaggt tcactatcgc cagcgcaccc
     7741 accagcagga agatcagcgt ggcaattgct gccgccagac cgaagtcctg acccccgccg
     7801 ccttcaaaag cgatacggta ggtgtagtta acaagcaggt cggtataacc ggctggcgtg
     7861 gtcgtgccaa gacgatccgg gccgccgttg gttaacagct gaatcagcac gaagttgtta
     7921 aagttaaagg cgaagctggc gatcatcagc ggcgtcagcg gtttaatcag cagcggcagc
     7981 gtaatcttaa agaagttctg gaacggacct gcgccatcca ttgctgaggc ttcatacaaa
     8041 tcgtccggaa tcgctttcag caagcccatg cagaggatca tcatgtacgg ataacccagc
     8101 caggtattga cgataatcag catcgtgcga gcggtggtcg gatcgctgaa ccaggcgggc
     8161 ttcacgccaa acagcgcgct caacatcatg ttgatttcac cgaagctctg gttaaacaat
     8221 cctttgaaaa tcaagattga aatgaacgat ggcaccgcgt agggcagaat cagcaggacg
     8281 cgatagaccg ctttgccgcg caacgcttcc cactgcacca gacatgccag aaccatgccg
     8341 accgccaccg ttaaaaagac agtgatcagc gagaacacca cggtccagac gaaaatggcg
     8401 aggaacggtt tctgaatgcc ttcgtcggta aagacgcggg taaagttttt ccagccggtg
     8461 gtcacggtgt aaccggggct tagcttttca tccccccagt tgccgtcggc ggtaatggac
     8521 tggtaaaagc caatttggtt attcggacga tatttcacgc cactctgatt attcgtcaac
     8581 gtgccgtcac cgtcgagtgt gtagagcggc tgcgtgccag aaaactggcg cagggagctc
     8641 atcatcactt tgttgccatc cggcagaatg gcggtaatgt cactcagcgc ctgacgattc
     8701 tgggtaatca cgcgcagatt cgcgcgttcg ccttcgggct gggcggtcgt ttctttcagt
     8761 tgcagttttt gctcgccgcc aaatttaaac gcgtcggaga ggtaattttt gccggtttcg
     8821 ccgtcgctga gcgccagttg ccactcatcg cccgccgggt aaagaccaaa gttataggtt
     8881 ttgcctgctt gccaggagcg atctaacaac acttcctgcg cacgttcaaa agtcagctgg
     8941 ttagtgctgc tgtagttggt gaaggcaatg gcgatggtgc agaccagagg gaagaggacg
     9001 aataatccca ttccagccat tcccgggtaa acatagcgcc aggcgtaggc tttacgattg
     9061 gcgaaaatat acagccccgc tgaactcaat atcagcgtgg taatggcgaa caggtattcc
     9121 ccttgtgcgt acattaaaac aacaaggtaa cccaccagca ggccgagcag acctagcact
     9181 gaccatttca gcgcgtcgct ttgccaccaa tgtttctttt taatgacatc catggggttc
     9241 ttcctcattc caggacggat aaggctttca cgccttatcc gacaacaact gcctgatgcg
     9301 acactgatgc gtcttatcag gcctacatgc gtttcagttt tgtaggccgg acaaggcgtt
     9361 cacgccgcat ccggcatttc acagcattac ttggtgatac gagtctgcgc gtctttcagg
     9421 gcttcatcga cagtctgacg accgctggcg gcgttgatca ccgcagtacg tacggcatac
     9481 cagaaagcgg acatctgcgg gatgttcggc atgatttcac ctttctgggc gttttccatg
     9541 gtggcggcaa tacgtggatc tttcgccaac tcttcctcgt aagacttcag cgctacggca
     9601 cccagcggtt tgtctttatt aaccgcttcc agaccttcat cagtcagcag atagttttcg
     9661 aggaactctt tcgccagctc tttgttcgga ctggcggcgt taataccggt gctcagcacg
     9721 ccaacgaacg gtttagatgg ttgacccttg aaggtcggca gtaccgttac accataattc
     9781 actttgctgg tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat cgctgtttcg
     9841 cctttattaa aggcagcttc tgcgatggag taatcggtgt ctgcattcat gtgtttgttt
     9901 ttaatcaggt caaccaggaa ggtcagaccc gctttcgcgc cagcgttatc cacgcccacg
     9961 tctttaatgt cgtacttgcc gttttcatac ttgaacgcat aacccccgtc agcagcaatc
    10021 agcggccagg tgaagtacgg ttcttgcagg ttgaacatca gcgcgctctt acctttcgct
    10081 ttcagttctt tatccagcgc cgggatctct tcccaggttt ttggcgggtt cggcagcagg
    10141 tctttgttat aaatcagcga taacgcttca acagcgattg ggtaagcaat cagcttgccg
    10201 ttgtaacgta cggcatccca tgtaaacgga tacagcttgt cctggaacgc tttgtccggg
    10261 gtgatttcag ccaacaggcc agattgagcg tagccaccaa agcggtcgtg cgcccagaag
    10321 ataatgtcag ggccatcgcc agttgccgca acctgaggga atttctcttc cagtttatcc
    10381 ggatgctcaa cggtgacttt aattccggta tctttctcga atttcttacc gacttcagcg
    10441 agaccgttat agcctttatc gccgttaatc cagattacca gtttaccttc ttcgattttg
    10501 gcgagagccg aggcggaaaa catcatcgtc gttaatgcgg ataatgcgag gatgcgtgca
    10561 cctgttttta ttttcataat ctatggtcct tgttggtgaa gtgctcgtga aaacacctaa
    10621 acggactcta gtttctttat acggcaacct ctttccatcc tccttgcccc tacgccccac
    10681 cgttgctttg tgtgatctct gttacagaat tggcggtaat gtggggatgc gcacataaaa
    10741 tcgccacgat ttttgcaagc aacatcacga aattccttac atgacctcgg tttagttcac
    10801 agaagccgcg ttctcatcct cccgcctcct cccccataaa aaagccaggg ggtggaggat
    10861 ttaagccatc tcctgatgac gcatagtcag cccatcatga atgttgctgt cgatgacagg
    10921 ttgttacaaa gggagaaggg catggcgagc gtacagctgc aaaatgtaac gaaagcctgg
    10981 ggcgaggtcg tggtatcgaa agatatcaat ctcgatatcc atgaaggtga attcgtggtg
    11041 tttgtcggac cgtctggctg cggtaaatcg actttactgc gcatgattgc cgggcttgag
    11101 acgatcacca gcggcgacct gttcatcggt gagaaacgga tgaatgacac tccgccagca
    11161 gaacgcggcg ttggtatggt gtttcagtct tacgcgctct atccccacct gtcagtagca
    11221 gaaaacatgt catttggcct gaaactggct ggcgcaaaaa aagaggtgat taaccaacgc
    11281 gtcaaccagg tggcggaagt gctgcaactg gcgcatttgc tggatcgcaa accgaaagcg
    11341 ctctccggtg gtcagcgtca gcgcgtggcg attggccgta cgctggtggc cgagccaagc
    11401 gtatttttgc tcgatgaacc gctctccaac ctcgatgctg cactgcgtgt gcaaatgcgt
    11461 atcgaaatct cccgtctgca taaacgcctg ggccgcacaa tgatttacgt cacccacgat
    11521 caggtcgaag cgatgacgct ggccgacaaa atcgtggtgc tggacgccgg tcgcgtggca
    11581 caggttggga aaccgctgga actgtaccac tatccggcag accgttttgt cgccggattt
    11641 atcggttcgc caaagatgaa cttcctgccg gtaaaagtga ccgccaccgc aatcgatcaa
    11701 gtgcaggtgg agctgccgat gccaaatcgt caacaagtct ggctgcctgt tgaaagccgt
    11761 gatgtccagg ttggagccaa tatgtcgctg ggtattcgcc cggaacatct actgccgagt
    11821 gatatcgctg acgtcatcct tgagggtgaa gttcaggtcg tcgagcaact cggcaacgaa
    11881 acccaaatct atatccagat cccttccatt cgtcaaaacc tggtgtaccg ccagaacgac
    11941 gtggtgttgg tagaagaagg tgccacattc gctatcggcc tgccgccaga gcgttgccat
    12001 ctgttccgtg aggatggcac tgcatgtcgt cgactgcata aggagccggg cgtttaagca
    12061 ccccacaaaa cacacaaagc ctgtcacagg tgatgtgaaa aaagaaaagc aatgactcag
    12121 gagatagaat gatgattact ctgcgcaaac ttcctctggc ggttgccgtc gcagcgggcg
    12181 taatgtctgc tcaggcaatg gctgttgatt tccacggcta tgcacgttcc ggtattggct
    12241 ggacaggtag cggcggtgaa caacagtgtt tccagactac cggtgctcaa agtaaatacc
    12301 gtcttggcaa cgaatgtgaa acttatgctg aattaaaatt gggtcaggaa gtgtggaaag
    12361 agggcgataa gagcttctat ttcgacacta acgtggccta ttccgtcgcg caacagaatg
    12421 actgggaagc taccgatccg gccttccgtg aagcaaacgt gcagggaaaa aacctgatcg
    12481 aatggctgcc aggttccacc atctgggcag gtaagcgctt ctaccaacgt catgacgttc
    12541 atatgatcga cttctactac tgggatattt ctggtcctgg tgccggtctg gaaaacatcg
    12601 atgttggctt cggtaaactc tctctggcag caacccgctc ctctgaagct ggtggttctt
    12661 cctcttttgc cagcaacaat atttatgact ataccaacga aaccgcgaac gacgttttcg
    12721 atgtgcgttt agcgcagatg gaaatcaacc cgggcggcac attagaactg ggtgtcgact
    12781 acggtcgtgc caacctgcgt gataactatc gtctggttga tggcgcatcg aaagacggct
    12841 ggttattcac tgctgaacat actcagagtg tcctgaaggg ctttaacaag tttgttgttc
    12901 agtacgctac tgactcgatg acctcgcagg gtaaaggtct gtcgcagggt tctggcgtcg
    12961 cgtttgataa cgaaaaattt gcctacaata tcaacaacaa cggtcacatg ctgcgtatcc
    13021 tcgaccacgg tgcgatctcc atgggcgaca actgggacat gatgtacgtg ggtatgtacc
    13081 aggatatcaa ctgggataac gacaacggca ccaagtggtg gaccgttggt attcgcccga
    13141 tgtacaagtg gacgccaatc atgagcaccg tgatggaaat cggctacgac aacgtcgaat
    13201 cccagcgcac cggcgacaag aacaatcagt acaaaattac cctcgcacaa caatggcagg
    13261 ctggcgacag catctggtca cgcccggcta ttcgtgtctt cgcaacctac gccaagtggg
    13321 atgagaaatg gggttacgac tacaacggcg atagcaaggt taacccgaac tacggcaaag
    13381 ccgttcctgc tgatttcaac ggcggcagct tcggtcgtgg cgacagcgac gagtggacct
    13441 tcggtgccca gatggaaatc tggtggtaat agcaaaacct gggccggata aggcgtttac
    13501 gccgcatccg gcataaaaac aggttgtcat tatctgaaag gggcgaaagc ccctctgatt
    13561 atcgggttta gcgcgctatt gcctggctac cgctgagctc cagattttga ggtgaaaaca
    13621 atgaaaatga ataaaagtct catcgccctc tgtttatcag cagggttact ggcaagcgcg
    13681 cctggaatta gccttgccga tgttaactac gtaccgcaaa acaccagcga cgcgccagcc
    13741 attccatctg ccgcgctgca acaactcacc tggacaccgg tcgatcaatc taaaacccag
    13801 accacccaac tggcgaccgg cggccaacaa ctgaacgttc ccggcatcag tggtccggtt
    13861 gctgcgtaca gcgtcccggc aaacattggc gaactgaccc tgacgctgac cagcgaagtg
    13921 aacaaacaaa ccagcgtttt tgcgccgaac gtgctgattc ttgatcagaa catgacccca
    13981 tcagccttct tccccagcag ttatttcacc taccaggaac taggcgtgat gagtgcagat
    14041 cggctggaag gcgttatgcg cctgacaccg gcgttggggc agcaaaaact ttatgttctg
    14101 gtctttacca cggaaaaaga tctccagcag acgacccaac tgctcgaccc ggctaaagcc
    14161 tatgccaaag gcgtcggtaa ctcgatcccg gatattcccg atccggttgc tcgtcatacc
    14221 agcgatggct tactgaaact gaaagtcaaa acgaactcca gctccagcgt gttggtagga
    14281 ccgctatttg gttcttccgc tccagctccg gttacggtag gcaacacggc ggcaccagct
    14341 gtggctgcac ctgctccggt gccggtgaag aaaagcgagc cgatgctcaa cgacacggaa
    14401 agttatttta ataccgcgat caaaaacgct gtcgcgaaag gtgatgttga taaggcgtta
    14461 aaactgcttg atgaagctga acgcctggga tcgacatctg cccgttccac ctttatcagc
    14521 agtgtaaaag gcaaggggta attacgcccc acagtgctga ttttgcaaca actggtgcgt
    14581 ctcctggcgc accttttttt atgcttcctt cctgggatat gagcgatttt ttatagtact
    14641 tcacttcttc ttcactaaga atgtccatta tttcaatgca ttatcagaga ttcttttcct
    14701 ttcgccggta gtgtctggac gctcaggcta cttttccagg ttattttatt tctgtattac
    14761 agaggtttta tgataggtca tatcacaaat tctggcggca ataactcttt gatgaaacat
    14821 gatgtggtgc aaggaaataa tatagtagat cttgatttac tacgtaattt caatggggtg
    14881 acaggtttaa acagagatta ctttatttat atcagtgata tatttttaaa tataaaaca
//
LOCUS       DAZPEU010000090        14681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPEU010000090 DAZPEU010000000
VERSION     DAZPEU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.091"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1247)
                     /locus_tag="ACHU2L_002463"
     CDS             complement(<1..1247)
                     /locus_tag="ACHU2L_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1357291.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKI"
     gene            complement(1445..1520)
                     /locus_tag="ACHU2L_002464"
     tRNA            complement(1445..1520)
                     /locus_tag="ACHU2L_002464"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1485..1487),aa:Phe,seq:gaa)
     gene            complement(1626..2333)
                     /locus_tag="ACHU2L_002465"
     CDS             complement(1626..2333)
                     /locus_tag="ACHU2L_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR1357292.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2486..2569
                     /gene="yqgH"
                     /locus_tag="ACHU2L_002466"
     CDS             2486..2569
                     /gene="yqgH"
                     /locus_tag="ACHU2L_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR1357293.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2731..4866
                     /gene="speC"
                     /locus_tag="ACHU2L_002467"
     CDS             2731..4866
                     /gene="speC"
                     /locus_tag="ACHU2L_002467"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1357294.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4915..6171)
                     /gene="nupG"
                     /locus_tag="ACHU2L_002468"
     CDS             complement(4915..6171)
                     /gene="nupG"
                     /locus_tag="ACHU2L_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR1357295.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6373..7452)
                     /gene="mltC"
                     /locus_tag="ACHU2L_002469"
     CDS             complement(6373..7452)
                     /gene="mltC"
                     /locus_tag="ACHU2L_002469"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR1357296.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7517..7792)
                     /locus_tag="ACHU2L_002470"
     CDS             complement(7517..7792)
                     /locus_tag="ACHU2L_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR1357297.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7820..8872)
                     /gene="mutY"
                     /locus_tag="ACHU2L_002471"
     CDS             complement(7820..8872)
                     /gene="mutY"
                     /locus_tag="ACHU2L_002471"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR1357298.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9033..9752
                     /gene="trmB"
                     /locus_tag="ACHU2L_002472"
     CDS             9033..9752
                     /gene="trmB"
                     /locus_tag="ACHU2L_002472"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR1357299.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9752..10078
                     /locus_tag="ACHU2L_002473"
     CDS             9752..10078
                     /locus_tag="ACHU2L_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR1357300.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10262..10981
                     /locus_tag="ACHU2L_002474"
     CDS             10262..10981
                     /locus_tag="ACHU2L_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR1357301.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11157..12203
                     /gene="ansB"
                     /locus_tag="ACHU2L_002475"
     CDS             11157..12203
                     /gene="ansB"
                     /locus_tag="ACHU2L_002475"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR1357302.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12320..13282
                     /locus_tag="ACHU2L_002476"
     CDS             12320..13282
                     /locus_tag="ACHU2L_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1357303.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13383..>14681)
                     /locus_tag="ACHU2L_002477"
     CDS             complement(13383..>14681)
                     /locus_tag="ACHU2L_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR1357304.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3830 a         3547 c         3786 g         3518 t
ORIGIN      
        1 ttaatctttg catattcaaa cgggctgata cccttctccc ggttcgcatc tagaaaatcg
       61 gcccaccatt gcaacatcag tcgccgttct tccagatgtt ctgctttatg gatatacgca
      121 gcacgtacac cattacgctc ctgatggctc atctgacgtt ccacagcatc gcgggaccac
      181 aaacctgatt caatcaatgc actgcacgcc atcgcccgga atccatggcc acagacttcc
      241 tgggttgtgt cataccccat caccctcagg gctttattta cagtattttc actgattggt
      301 ttacgcgggt catgtgcacc cgtaaagatc agaccatttt caccagccca tgtctgaagt
      361 tctgccagta tggctacaac ctgtttgctg agtggcacaa gatgtggtgt tcgcatcttg
      421 gatccccggt gtgagaattt tacgcctggc agaggttcgc gttcggctgg aatagtccac
      481 atggaattgt caatatcaat ttccgaccag cgggcatatc tgagttcact ggaacgaata
      541 aaaaccagta aattcagcat aactgccagt cgggtgacag gctggccttt ataaccgtca
      601 atacgcgaca gaagttcagg caggcgtgaa agctccagag cagggcgatg ctggcgtttt
      661 accgtggtca atgcaccagc catatccaga gccgggttat atctgatgat gccagactgt
      721 acggcgtaac gcattacagc agatattctt tgctgcagac gactggcgat ttcataaatt
      781 tgttttgctt ctgcagcacg aacagggata agcaggtccg gggtattgag tgttgtgata
      841 tcccggttgc cgataaaggg gaaaacgtgt gtctcgaggc ttttgagtac tttatcgctg
      901 tgtgattgtg cccattgttt attactggca tgccagcgtc gtgcaatatg ttcaaaagag
      961 agctgtaaat cagggacaac ttttttaact cttttctgtt cgcaagggtc aatcccctct
     1021 gaaatcagtt ttcgggcctc atcccgtttt gtacgagctt cggagagaga aacttctgga
     1081 tacacaccaa gtgccagagt cttctcctta cccagaatac gataacggag acgccagtaa
     1141 cgggaaccat taggatgaac cagcaggaac atgccggccc cgtcagtgag tttataagcc
     1201 ttgtcagtgg gctttgcagc ccggattttt gcgtcagtca gtgccatagt tcagccttct
     1261 gaatgcgtta aagagggtac aaattctgtc gaactggggt atacccgcaa ttatacccac
     1321 acaatggggt tgatgtgggg ggaatctggt tgagttcggt agaattgatt tggaggaaga
     1381 acacttaaat catggtgttc tgaaacgaaa aacggacctc cgtggaggtc cgttgatatg
     1441 aatttggtgc ccggactcgg aatcgaacca aggacacggg gattttcaat cccctgctct
     1501 accgactgag ctatccgggc aacggggcgc attaaacctg attcgcctcg tctcgtcaat
     1561 caaatttctt caatttactg cagactgcac aatctatcat cactttgccg ctattgcacg
     1621 cattgtcagg caaaccaggc ggtccaggcg gcggaaagcg gcattgccag aaggagtgct
     1681 ggaagcatgt tgaccaccgg gaacatttta atgccacaga tgcgtaatcc ggttgccagc
     1741 agcaataaac cgcctacggc gctgaagtct gccatcatcg acggtgtgat cagcggtaat
     1801 atcagcgcgg cagcccacgc cagcgttaac tggatgatca gtaatgggat actaattacc
     1861 gacaccgcaa tacccagcga gcaggcgaag atcatcgccg taaagaaatc aagaaatgac
     1921 ttggcgatta aaatactcgg atcgccggtc atcccttcgt tcatcgcccc gaagatcccg
     1981 gtgccgctgg cgcaaaacag gacaataatc gcgacataat tctgaataaa agattcatgc
     2041 gctggcttct tacgtgagtg acgaaacaga ttttgtgctt tggcgactgc tgtattgaca
     2101 cctttctcca gcaaacaaat ttcgccaatt agagcgccaa gtagcgtagc taaaaccatc
     2161 gctggaaggt tggcgcattt cacaaccagt aaaataccaa tccccagcga tgccagacca
     2221 aaaatagatg tcatggagac gcggatacgt tccggtaagc gttggctgag cagtgcgccg
     2281 agaacgccac ccagtaagac tgcactagcg ttgataaaag ggccgatgac cacaagagtt
     2341 cctgttagct gtattgtttg attttcttat tatgaacttt ttgccttgtt gatggcttgt
     2401 ctggtcgcaa agtcctggct tgcacgcctt agcgaaaggt gccatgattg cgcgaatttt
     2461 ctcctctctg tacggagttt gcccgatgca cgccatctcc ttacattctc tcgcttatcg
     2521 ccgtttcgcg cgaaacgttt ccctttctat gttactgctc atgcggtgaa gttaacgcac
     2581 gctcactgca ggacaacagt aaaatcagag cgtttctgct tttactgatg tctggcggtc
     2641 ggagctggtg accagtttga cccatatctc atggggcagg gttttccacc ttgtccggta
     2701 ttcttacttc cccgaaacgg gtttgcgctt atgaaatcaa tgaatattgc cgccagtagt
     2761 gaactggtat cccgactttc ttctcatcgc cgcgtggtgg cgttgggaga tactgatttt
     2821 acggacgtcg cggcagtcgt cattaccgct gcggatagtc gcagtggcat tcttgcgttg
     2881 cttaagcgca ccggttttca tctaccggtg tttttgtatt ccgaacatgc tgttgaatta
     2941 cctgcgggcg ttacggcggt aatcaacggc aacgagcagc agtggctgga gctggaatcc
     3001 gcagcctgtc agtatgaaga gaatttgctg ccaccgtttt atgacacgct gacgcagtac
     3061 gttgagatgg gcaacagcac ctttgcttgc cctggacatc aacatggtgc gttctttaaa
     3121 aagcatcctg ccggacgcca tttttacgat ttctttggtg agaacgtctt tcgcgccgat
     3181 atgtgtaacg ctgacgtaaa attgggcgat ctgcttattc atgaaggatc ggcgaaagat
     3241 gcgcagaaat tcgcagccaa agtctttcat gccgataaaa cctattttgt gctgaacggc
     3301 acatcggcag cgaataaagt ggtgacgaat gcgctgttaa cgcgtggcga tctggtgctc
     3361 ttcgaccgta acaaccataa gtcgaatcat cacggcgcgc tgattcaggc ggggacgacg
     3421 ccggtctatc tggaagcttc acgtaacccg tttggtttca ttggcggtat tgatgcgcac
     3481 tgttttaatg aagagtatct gcgccagcaa attcgcgacg ttgcgccaga aaaagccgac
     3541 ctgccgcgcc cgtttcgcct gacgattatt cagctgggaa cctatgacgg cactgtctat
     3601 aacgcccgtc aggtgatcga taccattggg catctgtgtg attacattct gtttgattcc
     3661 gcgtgggtcg gttacgaaca gtttatctcg atgatggcgg atagctcgcc gctgctgtta
     3721 gaacttaacg aaaacgatcc ggggattttt gtgacccagt tggtgcacaa acagcaggct
     3781 ggattctcac agacgtcgca gatccataaa aaagataacc atatccgcgg acaggcgcgt
     3841 ttttgcccgc ataagcggtt gaataacgcc tttatgctcc atgcttctac cagccctttc
     3901 tatccgctgt ttgctgcact ggatgttaac gccaaaattc atgaagggga gagtgggcgt
     3961 cggctgtggg ctgagtgcgt tgagttgggt attgagtcgc gcaaggctat tcttgcgcgc
     4021 tgtaagctgt tccgcccgtt tatcccgccc gttgttgatg gcaaattgtg gcaggattat
     4081 ccgacatcag tgttagccag cgaccgccgt tttttcagtt ttgagccggg ggcgaagtgg
     4141 cacggctttg aaggatatgc cgcggatcag tattttgttg atccgtgcaa gctgttactc
     4201 actacaccag gtatcaatgc cgaaaccggc gaatatagcg actttggcgt tccggcgacg
     4261 attctggcgc actatctgcg tgagaacggc attgtgccgg agaagtgcga tctcaactcc
     4321 attctgttct tattaactcc ggcggaaagc cacgagaagc tggcacaact ggtggcgatg
     4381 ctggcgcaat ttgaacagca tattgaggat gactcgtcgc tggctgaggt gttgccgagc
     4441 gtttataaca aatatccggt gcgctatcgc gactacaccc tgcgccagtt gtgtcaggag
     4501 atgcacgatc tgtatgtcag tttcgacgtc aaagacctac aaaaagcgat gttccgccag
     4561 cagagtttcc cgtcagtggt gatgaacccc caggatgcgc atagcgctta tattcgcggt
     4621 gaagtggagt tggtgcggat tcgtgatgcc gaagggcgaa ttgcggcaga aggggcgttg
     4681 ccttatccac ctggcgtgct ttgcgtggta cccggggaag tctggggtgg ggcggttcaa
     4741 cgttatttcc ttgcgctgga agaaggcgtg aatttgttgc cgggattttc gccggagttg
     4801 caaggtgtct atagcgaaac cgatgcggat ggcatgaaac gattgtacgg ttatgtgttg
     4861 aagtaagaat aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc gtaattagtg
     4921 gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca tcgccatgaa
     4981 cgcgaaggcc agaaccacgg aataaccagc gaaaatcaac catacggtct gccagtcggt
     5041 aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc cgaggataca
     5101 gccgaagccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa ttgccgggct
     5161 aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg cgcaaccgta
     5221 aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc cgtaagcaaa
     5281 cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct taataccgta
     5341 gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct gcgaaatcga
     5401 catgatgatt gacgcatgct gcacgatgaa gctgctggca aacatcggat ctttgtcgaa
     5461 gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc ccagcagcat
     5521 tgagaagatg aagaagattg ccatacgctt gtttttaaac agcgcgaatg catcgaggcc
     5581 cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat gcggcagagt
     5641 cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct gcatgtggct
     5701 taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga tggtgcccca
     5761 gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca ggcgatagta
     5821 agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa tcaatatcac
     5881 aaggaacatc gcttccggcg tcgtgacctg tgccgccatg aacagcgtga tagcgccaat
     5941 ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca caatccccag
     6001 cagcgcaggc ataaataccg cggcgatacc cagtgagcta taaactgcgc caatagaagc
     6061 accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc aacttcccca
     6121 cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca tgttaatttc
     6181 ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg agatgatggg
     6241 attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg caaacattcc
     6301 caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca tcgtcagggg
     6361 cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt tataaagata
     6421 acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat cgcccggcgt
     6481 catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc gcagcacgct
     6541 gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg ggttatcaat
     6601 tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat caatattgct
     6661 ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg agcggaacac
     6721 atctttcccg gcagtatttt gtaccacctg cattaatccc agcgcatcgg aacggctgac
     6781 cgcatacggg ttaaaggaag attcagtctg cataattgcc agaatcagcg actcatcaac
     6841 gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac gtttatcaag
     6901 gtggttcggc accatgttaa tggtgacgct gtagatgata cgtagcccgt tgctgcggct
     6961 tttcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt cccagcgaat
     7021 cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg aaattgtaat
     7081 atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca gcaacgtttt
     7141 gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatggttt cgatagtaat
     7201 cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt atttcacgta
     7261 gtccttagga ccggcgatca ccacctcttt gaagccccag atgttctcaa tattgtgggc
     7321 aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt cgttataggt
     7381 atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag ccagcgcgag
     7441 atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagct gccggaggcg
     7501 tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata tgcacctctt
     7561 taccctcgaa caggaagttg accatctcct gctccagcag cttgcggtgc tcggcattca
     7621 tcatgttgag tttcttttca ttaatcagca tggtttgttt gtgctgccac tgcgcccagg
     7681 cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac agctgaaaat
     7741 cctgaccttc tgcttcacgt tgcaggaaag tacaaaaaat cgttctgctc ataaatcatc
     7801 ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt aacaaacgct
     7861 ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag agcgcattgc
     7921 cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca atatctaagt
     7981 ggaaatggct gaaggtatgg cgaaacgcgg tcagttgcgt caggttatcg gcagcaatct
     8041 gccgttgtgc cagccaatgc cgcaaacttt cttcgtcggc aaactgcggg aaacagtata
     8101 aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct tcgtgctgta
     8161 acaggagaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg cccggataaa
     8221 gcgaccagct attgttggtg gcggcaatac atccgttttg tagcggacag agcgaacatt
     8281 tcggcttcga acgcgtgcaa atcatcgcgc ccaaatccat catcgcctga ttaaaccgtt
     8341 ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca tagtttattc tcgacctctt
     8401 ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg acgttaccgt
     8461 cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg gtggaacgcc
     8521 cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttacca ccgtgtaagg
     8581 tcgccacttg ttgtgccgct ttatgcaggt tgcgcgcgcg ggcgtaatag ccaagcccgg
     8641 tccacaagtg gagaacttcg tcgagcggcg cattggcgag atcggtcacc gtcgggaagt
     8701 gcgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt tgcaacatca
     8761 cttctgagag ccatactttg tagggcgtct tgtcaatttg ccagggcagc gttttccgcc
     8821 cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc atggtcaccg
     8881 acttcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta aaccggaact
     8941 ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat aatgcccgtt
     9001 ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt tcaccggaat
     9061 ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc caggggcgac
     9121 tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc gttgagttca
     9181 gtgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg acgcttgaga
     9241 ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgcccc gagcaggact
     9301 tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct gcgcatgaag
     9361 aaggtttaag caacctgcgc gtgatgtgtc acgatgcggt tgaagtgctg cataaaatga
     9421 ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg cacaaagcgc
     9481 gccataataa acgccgtatc gttcaggtgc cgtttgccga actggtaaaa agcaaactgc
     9541 agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa catatgcttg
     9601 aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat tacgtaccgc
     9661 gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt ggtcacggag
     9721 tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc gtcgtctgcg
     9781 taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat ggcgattccc
     9841 ggaaggtaca tcggaagaac agattgataa aatcgttgat gactttatta acgaggttat
     9901 cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag gtctgatctg
     9961 catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta agtggctgga
    10021 agagtgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt gggactaaga
    10081 aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat caggcctaca
    10141 gagatgcacg atcgagtagg cgggataagg cgtttacgcc gcatccggca tggaaaacgc
    10201 gtactttgtt atcaatctgg ggccagcata tgctggcctg atttgttctt gagggaagac
    10261 tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga ccgctcacgc
    10321 cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc aaaccgtgca
    10381 ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga tgtataacgg
    10441 taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc aggctgaact
    10501 acgtagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaga aagcccgtat
    10561 tgcgctggat aaaattatcg ttcaggagat gggcgacagc agcaaaatgc gcagccgtct
    10621 gaccaaactt gatgcgcagc ttaaagagca gatgaatcgc attatcgaaa cgcgcagcga
    10681 tggcctgacg ttccactata aagccattga tcaggttcgc gccgaaggcc agcaactagt
    10741 gaatcaggca atgggcggaa ttttacagga cagcattaat gaaatgggcg cgaaagcggt
    10801 gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcttgggcg gtctgcaatc
    10861 ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg gcaaagatgt
    10921 ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga atttaaaata
    10981 atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc gttttgttac
    11041 ctgcctctaa ctttgtagat ctccaaaata tattcacgtt gtaaattgtt taacgtcaaa
    11101 tttcccatgc aaagctaagg gataatgcgt agcgtccacg taactggagg aatgaaatgg
    11161 agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt gcagcattgg
    11221 cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt ggtgactccg
    11281 caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt aatgcggtgc
    11341 cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc ggctcccagg
    11401 acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacgccgac tgcgataaaa
    11461 ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaactgct tacttcctcg
    11521 acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc ccgtccacgt
    11581 ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgca gctgataaag
    11641 cctccgctaa tcgtggcgtg ctggtggtga tgaacgacac cgtactggac ggtcgcgatg
    11701 tcaccaaaac caacaccacc gacgtagcga ccttcaagtc tgttaactac ggtcctctgg
    11761 gatacattca caacggtaag attgattacc aacgtacccc ggcacgtaag cacaccagcg
    11821 atacgccatt cgatgtctct aagctgaatg agctgccgaa agtcggcatc gtttataact
    11881 acgctaacgc atccgatctt ccggctaaag cactggtaga tgcgggctat gatggcatcg
    11941 ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt gttcgacacc ctggcaaccg
    12001 ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt accgacgggt gctaccactc
    12061 aggatgctga agtggatgat gcgaaatacg gcttcgtcgc ctctggcacg ctgaacccgc
    12121 aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac caaagatccg cagcagatcc
    12181 agcagatttt caatcagtac taatcacctc gtcccggcat cgtgccgggg ctttttcact
    12241 tcagacttac gtccattgcc aattttcatt accctaatga taatcaccgg aataaattat
    12301 tccgcgcgag ggttttcggg tgaaaaagca atggattgtt ggcacggcgc tgcttatgtt
    12361 gatgactggt aatgcctggg cagatggcga accgccaact gaaaatatct taaaagatca
    12421 attcaaaaag cagtatcacg gcattctcaa gcttgatgcc atcagcttaa aaaatcttga
    12481 tgctaagggt aatcaggcca cctggtcagc ggaaggcgat gtctcttcca gtgacgatct
    12541 ctatacctgg gtcggtcagt tggcagatta cgaactgctc gaacagacct ggacgaaaga
    12601 taaaccggta aaattctcgg cgatgttaac cagtaaagga acgcccgcgt ctggctggtc
    12661 ggtgaacttt tactcttttc aggcggcagc cagcgatcgt gggcgggtgg tcgacgatat
    12721 caaaacgaat aataaatatc tgatcgtgaa tagcgaagat ttcaattatc gctttagtca
    12781 gcttgaatct gcgttgaata cccagaagaa ctcgattcct gcgctggaaa aggaagtgaa
    12841 agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg gatgcatatt ggggaaaaga
    12901 tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa aaaattcacc aacagcgtga
    12961 tgagtttaat aaacagaacg atagcgaggc gttcgcggtt aaatatgaca aagaggttta
    13021 tcaaccggcg attgcggcat gtcataaaca gagtgaagag tgttatgaag tgccgattca
    13081 gcagaaacga gatttcgata tcaacgaaca acggcgacag acttttctgc aatcacaaaa
    13141 actcagccgt aaattgcagg atgactgggt aacgctcgaa aaagggcaat atccgttaac
    13201 gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt ttgatgaaaa ttgacgatat
    13261 caaccaagct aatgaacgct agaaaaaaga taccgaacaa ctgcggcgca atggggtgat
    13321 taagtaactc atgttaagtg cctttgccgg ataatataaa aagttatccg gcaaattatt
    13381 ttttacatta gccctagtag ctgtggaatg aacaaggata tttgcggaat gtaagtgatc
    13441 atcatcaacg ctactagcaa tgcagcataa aatagcagca atggtttgat taaatgttga
    13501 atcttcacac ccgagataga acaaccaaca aatagcgcac tacccaccgg cggtgttaac
    13561 agcccaatac aaaggttggc caccatcata ataccgaaat gtactgggtc cattcccagc
    13621 tcttgtgcaa tcggcaagaa gatcggtgta aagattagta cagccggtgt catgtccata
    13681 aagatgccga caattaataa gacgatatta atcagcagca atatgatcag tgggttatca
    13741 gaaatcccca tcaatgcgtc gctgatcata taaggaatat cagcattagt cattgcccag
    13801 gacatgccca cggaaaaacc gatcaacagt aatacaatcg aagtggtaac aaccgactcc
    13861 agaatcagtt tgggtaaatg gcgccatttt acttcccggt aaatcagcac cgagagaata
    13921 aatgtgtaga caacggcaat tgctgacgct tcagttgcgg taaaaatgcc gcccagaatg
    13981 ccgcccatca caatgaacac cagaagcaaa cttggtaagg catccagaaa cgctttacag
    14041 gcctgagcaa aggttgctct ttctgaaagc gggtagccac ggcgtttagc aataatgccg
    14101 cagactatca ttaccgcaag acccattaag atccctggca aatatcccgc cataaacagc
    14161 gatgctacag aaacgccgcc agctgtcagg gaaaacacaa ttaacacgtt tgagggcggt
    14221 atcagtaatc cagtaataca agatgagacg tttacagcgg tagaaaatgc cggatcataa
    14281 ccttctttgg tctgaatggg attcagtgtg ccaccaacgg cagcagctgc cgcaacggct
    14341 gaaccagaaa tggaaccaaa catcatattc gccaggacgt tcacatgacc aagcgaacca
    14401 ggaacgcggc caaccattac ctgggcgagg ttaatcaaac gtatcgcaat accgccgctg
    14461 ttcatcagtg ttccggcaaa gatgaaaaag gggattgcca acagggcaaa gttatccagt
    14521 ccattagcca gtcgttgcga aacagtgatt gccgcgatat cccagggaaa catcaaaagc
    14581 attgaagcta cagtggcaat accgattgag aacgaaatgg gcacaccaat aaagaccagc
    14641 acaaaaaaac agccaaacag tgtaatcgcg atataactat c
//
LOCUS       DAZPEU010000091        14577 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPEU010000091 DAZPEU010000000
VERSION     DAZPEU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.092"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(77..2053)
                     /gene="speA"
                     /locus_tag="ACHU2L_002478"
     CDS             complement(77..2053)
                     /gene="speA"
                     /locus_tag="ACHU2L_002478"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR1357305.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2062..2193)
                     /gene="yqgB"
                     /locus_tag="ACHU2L_002479"
     CDS             complement(2062..2193)
                     /gene="yqgB"
                     /locus_tag="ACHU2L_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR1357306.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2287..2430)
                     /gene="yqgG"
                     /locus_tag="ACHU2L_002480"
     CDS             complement(2287..2430)
                     /gene="yqgG"
                     /locus_tag="ACHU2L_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR1357307.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2541..2792)
                     /gene="yqgD"
                     /locus_tag="ACHU2L_002481"
     CDS             complement(2541..2792)
                     /gene="yqgD"
                     /locus_tag="ACHU2L_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR1357308.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2848..4002
                     /gene="metK"
                     /locus_tag="ACHU2L_002482"
     CDS             2848..4002
                     /gene="metK"
                     /locus_tag="ACHU2L_002482"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR1357309.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4439..5833
                     /gene="galP"
                     /locus_tag="ACHU2L_002483"
     CDS             4439..5833
                     /gene="galP"
                     /locus_tag="ACHU2L_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR1357310.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5910..6407
                     /locus_tag="ACHU2L_002484"
     CDS             5910..6407
                     /locus_tag="ACHU2L_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR1357311.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6502..7209
                     /gene="endA"
                     /locus_tag="ACHU2L_002485"
     CDS             6502..7209
                     /gene="endA"
                     /locus_tag="ACHU2L_002485"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR1357312.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7289..8020
                     /gene="rsmE"
                     /locus_tag="ACHU2L_002486"
     CDS             7289..8020
                     /gene="rsmE"
                     /locus_tag="ACHU2L_002486"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR1357313.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8033..8983
                     /gene="gshB"
                     /locus_tag="ACHU2L_002487"
     CDS             8033..8983
                     /gene="gshB"
                     /locus_tag="ACHU2L_002487"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR1357314.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9092..9655
                     /locus_tag="ACHU2L_002488"
     CDS             9092..9655
                     /locus_tag="ACHU2L_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR1357315.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9655..10071
                     /gene="ruvX"
                     /locus_tag="ACHU2L_002489"
     CDS             9655..10071
                     /gene="ruvX"
                     /locus_tag="ACHU2L_002489"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR1357316.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10247..11227)
                     /locus_tag="ACHU2L_002490"
     CDS             complement(10247..11227)
                     /locus_tag="ACHU2L_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR1357317.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11245..11949
                     /gene="yggS"
                     /locus_tag="ACHU2L_002491"
     CDS             11245..11949
                     /gene="yggS"
                     /locus_tag="ACHU2L_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR1357318.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11967..12533
                     /gene="yggT"
                     /locus_tag="ACHU2L_002492"
     CDS             11967..12533
                     /gene="yggT"
                     /locus_tag="ACHU2L_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR1357319.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12530..12820
                     /gene="yggU"
                     /locus_tag="ACHU2L_002493"
     CDS             12530..12820
                     /gene="yggU"
                     /locus_tag="ACHU2L_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR1357320.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12828..13421
                     /locus_tag="ACHU2L_002494"
     CDS             12828..13421
                     /locus_tag="ACHU2L_002494"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR1357321.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13414..14550
                     /gene="hemW"
                     /locus_tag="ACHU2L_002495"
     CDS             13414..14550
                     /gene="hemW"
                     /locus_tag="ACHU2L_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR1357322.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3513 a         3856 c         3749 g         3459 t
ORIGIN      
        1 ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa tcgacccgcc
       61 agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa cctgcctcga
      121 actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc acttgatcgc
      181 ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg tccgccacgg
      241 tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg aacacgtcaa
      301 ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga tatgcaccga
      361 ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt ggcattgtcg
      421 tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca caggtaatat
      481 ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt agaaccggga
      541 acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa ttgacgtaca
      601 ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca cggttttgcg
      661 gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct gcccatgcac
      721 gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc agatccatct
      781 ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc atttcctgcc
      841 aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg gtcggcaccg
      901 tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg tgcgcagtca
      961 ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct tcacacgcat
     1021 cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt acggagcagt
     1081 cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg tcgaagcact
     1141 gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca cgaacgcctg
     1201 tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt agttgcaggc
     1261 tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga gtcgcagcca
     1321 ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc tgcgaagcca
     1381 gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca tccagcacaa
     1441 tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc ttctcgccaa
     1501 ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg acgctacgag
     1561 tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct tccagaccca
     1621 gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta actttgatcg
     1681 gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt ttgaacgcgg
     1741 cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt gcaggcagac
     1801 gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga gcttccggga
     1861 cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag tagttattgc
     1921 cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag ctcattgcaa
     1981 cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa cccatagaca
     2041 tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt taacgactgt
     2101 cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca gtgtatgctg
     2161 acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat aaccgaacgt
     2221 aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag cgcccttgtt
     2281 cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat gggtataaca
     2341 tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac cagaacggtg
     2401 agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg gccgcctgtt
     2461 aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc aacacgaaag
     2521 aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac aatggcagaa
     2581 aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc cagccacgtt
     2641 tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa ttgacctaaa
     2701 atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc agtgttttta
     2761 acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag tttgagctaa
     2821 ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc cgagtccgtc
     2881 tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt agacgcgatc
     2941 ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac cggcatggtt
     3001 ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat cacccgtaac
     3061 accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa ctcctgtgcg
     3121 gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga ccgtgccgat
     3181 ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac taatgaaacc
     3241 gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg tcaggctgaa
     3301 gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca ggtgactttc
     3361 cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac tcagcactct
     3421 gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat caagccaatt
     3481 ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac cggtcgtttc
     3541 gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat cgttgatacc
     3601 tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc atcaaaagtg
     3661 gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc tgctggcctg
     3721 gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc gacctccatc
     3781 atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct gctggtacgt
     3841 gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct gcacccgatc
     3901 tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg ggaaaaaacc
     3961 gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt cacctgcgtt
     4021 caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat aggcgttcac
     4081 gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat cacgtttcac
     4141 ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa atatttgtaa
     4201 tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt aaagtcttaa
     4261 ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat gcccttaaat
     4321 cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct tcacatcttt
     4381 ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg agggcatcat
     4441 gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg tctgcttcct
     4501 tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg gcgcactgcc
     4561 gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg taagctccat
     4621 gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta aactcgggcg
     4681 caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt tctctgcggc
     4741 tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg cggtgggtgt
     4801 ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa ttcgcggcag
     4861 tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt atctttctga
     4921 taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta tcatcccggc
     4981 aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt ttgccgccaa
     5041 acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca gcgcggaagc
     5101 gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg gctgggcgct
     5161 gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt tacaggtaat
     5221 gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct tcgaactggc
     5281 gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc tgaccaacgt
     5341 acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac caacgctaac
     5401 gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga tgcatatcgg
     5461 tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt ttattgtcgg
     5521 ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc agccgctgaa
     5581 aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca acatgatcgt
     5641 tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct tctgggtgta
     5701 tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg aaaccaaaca
     5761 cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc gcgaaatcgg
     5821 cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc tcttgcagtc
     5881 atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct atcgccatcc
     5941 aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg aagctagggc
     6001 gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc ggaacagcct
     6061 ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac agtgaagctt
     6121 ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa catttcggcc
     6181 gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg ggcgttcccg
     6241 cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc ccctaccgct
     6301 gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt cgtggcgagg
     6361 ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca cctgaactat
     6421 caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac gttgctggct
     6481 cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt ggtactgagc
     6541 gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca ggcgaaagcc
     6601 gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg taaaattaac
     6661 tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt gcgcaaaaat
     6721 gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca attcggtcac
     6781 cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc ggtctatcgc
     6841 aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa tggcgatcgc
     6901 ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca atgcgccatg
     6961 aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg tgccattgcg
     7021 cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca gcaaacgcag
     7081 ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga gcgcgatgaa
     7141 cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg ccaggcgcga
     7201 aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg cgtacaaccg
     7261 cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc atcctgaacc
     7321 actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata tcgggcgcgt
     7381 actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc aggtctttga
     7441 cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg aaggccagat
     7501 cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc gtggtgaaaa
     7561 aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta cgccactttt
     7621 ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc ttcagcagtg
     7681 gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc cggaaatccg
     7741 tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga aactgaatct
     7801 tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac gcgtccgcct
     7861 gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg cccgctatca
     7921 atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg cgctcaccgc
     7981 cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa taatgatcaa
     8041 gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt ccagtttcgc
     8101 tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga tgggcgatct
     8161 gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga agcagaacta
     8221 cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc tcgatgtgat
     8281 cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct atattctgga
     8341 acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc gcgactgtaa
     8401 cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg ttacgcgcaa
     8461 taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta agccgctgga
     8521 cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc tcggcgtgat
     8581 tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt acctgccagc
     8641 cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac cgtactgcct
     8701 ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg gtcgcggtga
     8761 acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc cgacgctgaa
     8821 agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga ctgaaattaa
     8881 cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt cgatcaccgg
     8941 aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct tagcgagaag
     9001 gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg gcgctgttgc
     9061 ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact ttcttattgc
     9121 catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt gcgaacataa
     9181 taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca aaattgaagg
     9241 gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc gtctggataa
     9301 accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc atactccgcc
     9361 ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca cttcccgcga
     9421 tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg ctctgggcta
     9481 tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt ggctaacggc
     9541 cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc gcgaggcggc
     9601 aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg cctgatgagt
     9661 ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt cggccaacgc
     9721 attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac gccggactgg
     9781 aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt cggtttgccg
     9841 ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt tgccaaccgt
     9901 attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag cactgtggaa
     9961 gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg caaagttgac
    10021 tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta aggcgatcta
    10081 aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac gccttatcca
    10141 gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca tcagacattt
    10201 attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac aaacgccctt
    10261 cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc tgcccggttt
    10321 gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc accgcgggag
    10381 tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact tccagctttt
    10441 gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct ttttcctgcg
    10501 ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta tgtaaggttg
    10561 ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc tcactgtcac
    10621 gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc aaccccgatg
    10681 cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc tggctggcat
    10741 agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt tgattaagat
    10801 agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc gtcaccagaa
    10861 tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca agctgttcga
    10921 gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc cgttgcgcga
    10981 acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc agctggccat
    11041 tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt
    11101 ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt gcgggccagg
    11161 cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca atttcttcca
    11221 tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc acaggtccgg
    11281 gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat tacgctgctt
    11341 gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt
    11401 caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt tcaggaactg
    11461 ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa aagccgccta
    11521 gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc
    11581 aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat taatattagt
    11641 gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc agcggcggtc
    11701 gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct tgagtcagaa
    11761 tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg actgaaaacg
    11821 cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga agccgccatt
    11881 gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg tgattactct
    11941 aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt tcaacggtca
    12001 ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct cattgtgatt
    12061 tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc gggccactgc
    12121 gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt gcctatattc
    12181 tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca atcatctgga
    12241 ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg gtcctgctgg
    12301 tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac gtgctgattc
    12361 agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg ggtgggattg
    12421 atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt gtcgcagaag
    12481 tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta tgagtgccgt
    12541 aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag ccagccgtga
    12601 ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc cgccggttga
    12661 cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg ttgccaaaag
    12721 ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc
    12781 gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg caaaaagttg
    12841 tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt agcgactttg
    12901 gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa accggcctaa
    12961 cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc ggtttaccgg
    13021 caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg ggtatttact
    13081 ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa ctgttggaaa
    13141 cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc
    13201 tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg ggcgtgatta
    13261 ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc gtaccttccg
    13321 aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc caccgtggtc
    13381 aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc gctgagtctc
    13441 tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg
    13501 ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga tctggacaat
    13561 gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg tggtacgccg
    13621 agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc gcgtttgccg
    13681 ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga agcggatcgc
    13741 tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca gagttttagc
    13801 gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa acgagcggca
    13861 aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca tggactaccg
    13921 gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact gaatccgccg
    13981 catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc gcgaccgccg
    14041 gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca gttattaacc
    14101 gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta tcagtgccag
    14161 cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg cgcgcacggc
    14221 aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca tcctcgtggt
    14281 tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga taagccgttt
    14341 gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga gtttattgcg
    14401 tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat tgcccagggt
    14461 tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt attcttaaac
    14521 tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc gcattaa
//
LOCUS       DAZPEU010000092        14537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPEU010000092 DAZPEU010000000
VERSION     DAZPEU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.093"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            211..777
                     /locus_tag="ACHU2L_002496"
     CDS             211..777
                     /locus_tag="ACHU2L_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1357323.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            828..3287
                     /locus_tag="ACHU2L_002497"
     CDS             828..3287
                     /locus_tag="ACHU2L_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1357324.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKIPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DGNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3302..4033
                     /locus_tag="ACHU2L_002498"
     CDS             3302..4033
                     /locus_tag="ACHU2L_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1357325.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            4030..5106
                     /locus_tag="ACHU2L_002499"
                     /pseudo
     CDS             4030..5106
                     /locus_tag="ACHU2L_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5258..6304
                     /locus_tag="ACHU2L_002500"
     CDS             5258..6304
                     /locus_tag="ACHU2L_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR1357326.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6301..7092)
                     /gene="nei"
                     /locus_tag="ACHU2L_002501"
     CDS             complement(6301..7092)
                     /gene="nei"
                     /locus_tag="ACHU2L_002501"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR1357327.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7128..7862)
                     /gene="pxpA"
                     /locus_tag="ACHU2L_002502"
     CDS             complement(7128..7862)
                     /gene="pxpA"
                     /locus_tag="ACHU2L_002502"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR1357328.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7852..8784)
                     /gene="pxpC"
                     /locus_tag="ACHU2L_002503"
                     /pseudo
     CDS             complement(7852..8784)
                     /gene="pxpC"
                     /locus_tag="ACHU2L_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(8778..9434)
                     /gene="pxpB"
                     /locus_tag="ACHU2L_002504"
     CDS             complement(8778..9434)
                     /gene="pxpB"
                     /locus_tag="ACHU2L_002504"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR1357329.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(9457..10200)
                     /gene="ybgI"
                     /locus_tag="ACHU2L_002505"
     CDS             complement(9457..10200)
                     /gene="ybgI"
                     /locus_tag="ACHU2L_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR1357330.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10471..11952
                     /gene="dtpD"
                     /locus_tag="ACHU2L_002506"
     CDS             10471..11952
                     /gene="dtpD"
                     /locus_tag="ACHU2L_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR1357331.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(12102..13520)
                     /gene="phrB"
                     /locus_tag="ACHU2L_002507"
     CDS             complement(12102..13520)
                     /gene="phrB"
                     /locus_tag="ACHU2L_002507"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR1357332.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13517..14026)
                     /locus_tag="ACHU2L_002508"
     CDS             complement(13517..14026)
                     /locus_tag="ACHU2L_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1357333.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            14155..14442
                     /locus_tag="ACHU2L_002509"
     CDS             14155..14442
                     /locus_tag="ACHU2L_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR1357334.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
BASE COUNT         3563 a         3880 c         3516 g         3578 t
ORIGIN      
        1 tctcagttac atgaataaac caccaaatat agcgcacccc tataattccc aatactttat
       61 tcatcaaaca tttaattcat accatcaatt aattactttt acatttaata aaattaataa
      121 tatttttaag aaaaactaaa aacattcgac aatcaaataa gatattcttg caccgatata
      181 cttttttctt ttctgttaaa ggactcagtt atgtttaaag gacaaaaaac attggccgca
      241 ctggccgtat ctctgctgtt cacagcacct gtttatgcgg ctgatgaagg atccggtgaa
      301 attcacttta aaggtgaagt tattgaagca ccgtgtgaaa tacatcagga tgatattgat
      361 aaagaggttg aactcggtca ggtgaccacc agccacatta atcagtcaca tcacagcgat
      421 gccgttgctg tcgacctgct cttagtcaac tgtgatctgg aaaactccag caacggttcc
      481 ggtggcaaga tttctaaagt tgcagtcacc tttgatagct cagcgaaaac caccggcgca
      541 gatccgattc tcaacaacac cagcacgggt gaagccaccg gcgtcggcgt acgtttaatg
      601 aataaagatc aaagcaatat cgttttaggc accgctactc cagatatcga cctggctccg
      661 acctccagcg aacaaacgct gaatttcttt gcctggatgg aacaaattga tcaggctaca
      721 cctgtaacgc caggcgcagt tacggcgaat gcaacctacg tgctggatta taaataagta
      781 ttactttggc ggcgctatcg ccgcctttca taaaatattt atcaggtatg gacaccgtga
      841 atatttatcg actctctttt gtatcctgcc tggtcgtggc gatgccttgc gcattggcgg
      901 tcgaattcaa tctgaatgtt ctcgataaat caatgcgcga ccgcattgat atttcattat
      961 taaaggaaaa aggagtcatt gctcccggtg agtattttgt tagcgttgcg gtaaataata
     1021 accaaatcag taacgagcaa aagattaact ggcacaaaaa tgacgataaa accattccgt
     1081 gcatcaatga tttactggtc gataaatttg gcttaaaacc tgaagtccgt cagtcgttac
     1141 cattgataaa tcagtgcgtc gattttagct cccgacctga aatgctcttc aatttcgatc
     1201 aagccaatca gcaactgaat attagtattc cgcaagcctg gctggcgtgg cactcagaaa
     1261 actggacccc accctccaca tggaaagaag gtgtcgccgg tgtcctgatg gattacaact
     1321 tgtttgccag cagctaccgc ccacaggatg gcagcagcag cactaatctt aacgcctatg
     1381 gaacagccgg aatcaacacg ggggcatggc gcttacgcag tgattatcag ttgaatcata
     1441 ctgatagcga tgataaccat gaacagtcag gcgaaatatc acgtacctat ctttttcgcc
     1501 cgttaccgca attaggttca aaattaaccc tcggcgaaac ggattttagt cccaatattt
     1561 tcgacggttt ttcttatacc ggcgcggcac tggcaagtga cgatcgaatg ttgccatggg
     1621 aactacgcgg ctacgcccca caaattagcg gtattgcaca gaccaatgcc acggtgacga
     1681 tcagtcaatc aggccgcgtc atttaccaga aaaaaatccc accaggccca tttatcattg
     1741 acgaccttaa tcagtctgtt cagggcacac tggatgtcaa agtgacggaa gaagatggtc
     1801 gggtgaacaa tttccaggtt tcagcagcat cgacgccctt cctgactcgt cagggacagg
     1861 ttcgctataa actggccgcg ggtcagccac ggccctccat gtcacatcaa actgaaaatg
     1921 aaaccttttt tagcaatgaa gtttcctggg ggatgctgtc aaacacctcg ctgtacagcg
     1981 gcctgctgct ttctggtgat gactaccatt ctgccgcaat gggtattggg caaaatatgc
     2041 tgtggcttgg tgcgctgtcg tttgatgtca cgtgggccag tagccatttt gatactcagc
     2101 aggacgagcg gggcttaagc taccgtttta attacagcaa acaagtggat gccactaaca
     2161 gcacgatttc cctcgccgct tatcgtttct ccgatcgtca ttttcacagc tacgccaact
     2221 atctggatca caaatacaac gacagcgatg cgcaggacga aaaacagacg atcagcttat
     2281 ctgtgggcca accgattacc ccactaaacc tcaatcttta cgccaacctg ctacatcaaa
     2341 cctggtggaa tgcagacgcc tccacgaccg ccaacatcac ggcaggtttt aatgttgata
     2401 ttggtgactg gagagatatc tctatttcta cgtcattcaa tacaacccat tacgaagata
     2461 aagatcgcga caaccagatt tacctgtcga tttcgctccc cttcggtaac ggtggtcggg
     2521 ttggctatga catgcaaaac agtagccaca gcaccacaca ccgcatgtcg tggaacgata
     2581 cgctggatga acgtaatagc tggggcatgt ctgccggact gcaatccgac cgtcctgaca
     2641 atggagccca ggtgagcggt aactatcagc acctgagttc agcgggtgag tgggatattt
     2701 ctggtaccta tgccgccaat gattacagtt ccgtcagcag cagctggagc ggttctttca
     2761 ccgcaaccca atatggtgca gcgtttcatc gccgcagctc caccaatgaa ccacgcctga
     2821 tggtcagcac cgatggcgtg gcagatattc cggttcaggg caatctcgac tacaccaacc
     2881 attttggcat tgcggtggtg ccgttgattt ccagttacca gccttccacc gtggcggtga
     2941 acatgaatga cttacccgac ggcgtaacag ttgcagaaaa cgttatcaaa gaaacgtgga
     3001 ttgaaggcgc gataggttac aaatcactgg cttcccgttc cggtaaagac ggtaacgtca
     3061 tcattcgcaa cgccagcggt cagtttcctc ccctcggagc ggatatccgc caggatgaca
     3121 gcggcattag cgtggggatg gttggcgagg aaggacatgc ctggttaagc ggagtcgctg
     3181 aaaatcaaaa gtttaccgtg gtctggggtg atagccagca ttgctcgctc catcttcctg
     3241 aacatatgga agacaccgca aatcgcctga ttttaccttg tcattaatta aggaaatgaa
     3301 aatgacattt atgaaaggac tacccctttt gttgttggtt gccagtctgt gcagccacgc
     3361 tgcactacaa cccgatcgca ctcgtattgt gtttaacgct aatgataaag ctaccagtct
     3421 gcgggtggat aatcgtagcg ataaactccc ctacctcgct tattcctggc tcgaaaatga
     3481 aaaaggggag aaaagcgatg atcttctggt cgccctgccg ccgatacaac gtctggaacc
     3541 gaaagcaaca acacaggtac ggatagtaaa gcaagcctcg accacaaaat taccgggcga
     3601 tcgcgaaacg ctatttttct acaatatgcg tgaaattcct cccgccccgg aaaaaaacag
     3661 cgaccatgcc gttctgcaag tcgccattca gagtcgtatc aaggtgttct ggcgaccggc
     3721 tgcattacgc aagaaagcag gagaaaaggt cgaactgcag ttacaggtca gccagcaggg
     3781 caaccaactg acactgaaaa atcctaccgc gtattatctg accattgcct acctgggacg
     3841 aaatgaaaaa ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt ttagtacggt
     3901 caacacaaac accggaaatt atagcggcag ccagttttat ctcggttata tggatgatta
     3961 cggcgcgtta cgcatgacga cgctcaactg cagcggacaa tgccatttac aagccgtgga
     4021 ggcgaagaaa tgagtgctgg caagggagtg ttgctcgtca tctgtctgtt atttctgtcg
     4081 ttgaagtctg cgcttgccct gaactgctac tttggttcat ccggtggctc ggtagaaaaa
     4141 tcagaagcga ttcaaccttt tgctgtacca ggcaatgcca aactcggcga taagatctgg
     4201 gaatctgacg atattaaaat aagatctggg aatctgacga tattaaaatt cccgtctatt
     4261 gtgacaacaa taccaacggc aactttgaaa gcgaacatgt ctacgtatgg gtaaacccct
     4321 acccgggggt gcaggatcgt tattatcaac tcggagtaac ttacaatggc gttgattatg
     4381 acgccaacca gggaaaaagc cgcatcgaca ctaaccagtg tatcgacagt aagaacattg
     4441 acatttatac ccctgatggg atggcagaac aaaatttgct cgggtgatcc cgctaatatt
     4501 cacatgtcgc gaacctttct cgctcgcatg cggttatacg tcaaaatacg agaaatgccg
     4561 ccgcatgatt atcaaagtac gcttagcgac tatatcgtcg tgcaatttga cggtgccggt
     4621 agcgttaacg aagaccctac tgcccaaaac ctgaaatatc atattactgg tctggaaaac
     4681 attcgcgtgc tggattgcag cgtcaatttt tccatttcac cggaaacaca gatgattgat
     4741 tttggtaaat ttaatttgct ggatattcgt cgccacacaa tgtcgaaaac attcagtatt
     4801 aagacgacaa aatcacaaaa tgaccaatgc accgacggat ttaaagtcag ttcctccttt
     4861 tataccgaag aaacgttggt tgaagaagat aaagcattgt tgattggtaa tggtctgaag
     4921 ctacgtttgc tggatgaaaa cgcctcgcct tataccttca ataaatatgc tgaatacgcc
     4981 gatttcacca gcgacatgtt ggtctacgaa aaaacctata cagctgaact ttcgtccatt
     5041 gcaggtaccc ccatcgaggc tgggcccttc gatacagtgg tgctttttaa aattaactat
     5101 aactgatttg ccatgatgcc ctttgcagcc ggaaagggca tctctttcag aaaattgctg
     5161 attttcccct tttccttgat tgctgttatt ttttcctagc ccggctttct ttatgctgta
     5221 tcgatacacc aaagagaaaa taatgaggga gcgaaggatg ccagttttgc agtggggaat
     5281 gttatgtgtg ctatcacttc tcctttctat tggtttcctc gcggtacatc tcccggcggc
     5341 gctattactt gggccgatga tcgccgggat catcttcagt atgcgcggaa ttactctgca
     5401 acttccccgc tccgcttttc tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa
     5461 cctcactggt tcgattctca ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt
     5521 actggtgacg ctgctttcca gcgccatcgt gggttggtta ttggtgcgct atagctcact
     5581 gcctggaaat accggtgcct ggggttcttc tccaggcggc gcagcggcaa tggtcgctat
     5641 ggcgcaagat tacggcgcag atattcgcct ggtggcgttt atgcaatatc tgcgagtgct
     5701 gttcgtcgcg ggggccgctg ttctggtcac ccgcatgatg ctgggtgata acgctgaagc
     5761 ggttaaccag caaatcgtct ggttcccgcc agtgagcatt aatctcctgc ttacgatttt
     5821 gctcgcagtc gttgccggta cggcgggatg catgctgcgt cttccttccg gcacgatgct
     5881 catcccgatg ctggcgggcg cagtgctcca gtctggtcag ctcatcacca tcgaactacc
     5941 ggaatggctg ctggcgatgg cgtatatggc aattggctgg cggattggtc ttggtttcga
     6001 taaacaaatc ttactgcggg cactacgccc gctaccgcaa atcctgctgt cgatttttgc
     6061 tctgctggct atttgtgcag gtatggcgtg gggactgacc cggtttatgc atattgattt
     6121 tatgactgcc tacctcgcca ccagtcccgg cgggcttgat accgtggcgg tcatcgccgc
     6181 agggagcaat gccgatatgg cgcttattat ggcgatgcaa accctgcgcc tgttcagtat
     6241 tttgctgacg gggccagcca ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc
     6301 ctagtgctgg cagcctggac accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat
     6361 gatgccgcca cagcgttcgc acagttcgcc atcacgatga aaaaccttaa agcgaaacag
     6421 cgccccatga tgcttattct catccacctg cccccgcgta gcgtaagaaa accgaggaat
     6481 ctccagtaac gcgtgtgcga gtgcatccag ttgcgccgca ttgagatctt tcgctttatg
     6541 atttccagtc aacccaacct gccagaggat ctccacccgc aaataattgc caagcccagc
     6601 cagaaacgcc tgatcgagca gtaatcctgc aaactgacgg ttacgaaagc gcggcgacaa
     6661 taaccgttcc tttaccacct ccggcgtcag attcggatcc agcacatcgg gaccaacgcg
     6721 ttgtaaaaac ggatgcgtgg tcagttgttc cggcctcaac atctcaatat ccgaggcgct
     6781 ataaagcaga atggttttgt cagccgtttg cagttttacc cgcaatactc gcgtggtctg
     6841 cggctcttcg ccggtatcaa ccacgcgcca gacgccgtaa agctgattat ggctgtagag
     6901 cgttaaaccg ttgggaaaat gagttaacaa cgccttacca cgcgtttcca catgggtaac
     6961 gtgttgaccg ataagttgtg attgataagt ttttaactgc gggaaggcaa accagacatc
     7021 agttagtggt ttgcctttga tcgccgcctc caggttatcc gctgcacggc ggatctccgg
     7081 gccttcaggc atggtgatat ccttttttca ttctttatta agcggggtta tgctgcgaca
     7141 acaatcccct ttttggcaaa tgtagctcgt aatcggcggg cgaacgccag agcgtgctcg
     7201 ccgtcgccat gcaggcagac cgtttgcgcc gcgaccgttg cccattcgcc ggtgatactt
     7261 ttgactctgc cgtgttgcac catctccagc gtttgcgcca acgcctgttc ttcgttttca
     7321 atcaacgcgc ctggctgact tcgcggcacc agcgagccgt cagcctgata accgcgatcg
     7381 gcaaacactt cctcgcgcgt tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt
     7441 cctgccagcc caacgagaac caatgccgga tcgcaagcgt ataccgctct ggcgatggcg
     7501 tctgccagtt gtgcttcttt cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga
     7561 cgcattacgc cgccttgcgc acgggcaatc gttgccagcg cgccaatttg atacagcgtc
     7621 tgggcgtaaa cggtttccgg cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg
     7681 ggaaaactcg ggtgagcgcc aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc
     7741 tgcatggttt gcgcatcgcc tgcatgaaat ccacaagcaa tattggcaga ggaaaccagc
     7801 gttaatagct ctgcgtcgct ggcgcagcct tcgcccagat cggcgttcag gtcaattttc
     7861 attgtgcagc cgccacgcta attgttcaaa ataacgttgc tgatcttgcc gcgctttcag
     7921 tgcctcttcc agtgaacact ggacaaaatg aatcggctga ccgagcggaa tttgcgccag
     7981 atggtacata tcagcctcaa tgatacaggc aatacgtgga taaccaccgg tggtctgtgc
     8041 gtcgttcatc aacacaatcg gctgcccgtt atgcggcacc tgcaccacgc ccggtaacaa
     8101 accgtgagat aacagttcgc gatcggtgat gcgttttaaa atttgcccct gtaaacgata
     8161 gcccattcgg ttactttgcg agctaagctg ccagggcgaa cgccagaatg catcctgcga
     8221 ggcgcgatcg aactcatgat attccggccc cggcaaggcg cgaatgcggt tgccccacag
     8281 cagctgttta acgccctgcg cttccataaa atcacgcttc gatttgccaa tcgggagtca
     8341 gtcaccatcc ttcagtaaac ggccttccag cccgccaatc cccactttga gatcggtgct
     8401 gcatgacccc attaccggcg gaacatcaat accacccgcg accgccagat aactgcgcat
     8461 cccgtgctgc gggcgtttaa gcgttaaacg ctggcctgct ttcatcggca atcgccagcc
     8521 ggtccagacg gcgttatcat ccaaccgcgc ttcgcaacct gcacccgtca gggcaaacca
     8581 cccatcagtt tcgaactcaa ctgttaactg accgagcgtg atctccagcg cgggggcatt
     8641 ggcgtcatta cccaccagca ggttagcaat gcgcagcgca ggcatatcca gtgcgccaca
     8701 gtggctgata cccgactggc aaaaaccgtg acgaccgcca tcctgcacag tggtgtacat
     8761 gcccgcacga ataatcttca gcatattccc tccttctgcg gtacaaatcg cacgctgtct
     8821 cccggacgta ataagatggg ttcgtcacgc gccggatcaa acaggctgag tgaggtatga
     8881 ccaatcaact gccagccacc cggcgttgcc agcggataaa caccagtctg cggcccgccg
     8941 atcccgacag aacctgccgg aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt
     9001 tgttccggca aactcccgag atacgggaag cccggttgaa aacctaaaaa ccagaccacg
     9061 tattccacgg aggagtgcaa ttcaacaacc tgtttttcgc tcaacccgca atacgccgcg
     9121 accaccgcca aatccggtcc gcctgcacca ccgtaaacca ccggaatttc aataaagcga
     9181 gactcaggtt ccagggcctc gctctcctcc caccagcgtt gcaaacgctc tatggcatcc
     9241 agcgccagcg actcaggatt acgcaaaatc accgtgatat tgttcatgcc gggaatggct
     9301 tcaaccacat tcggcatatc caccagacgc tgcgccagtc gccagatccg tttctggcta
     9361 gccagcgtca ccggcggttc cagttccagc actaccgccg tttcacctat cagataacaa
     9421 cgcgctcgtt gcactttcga tccctctgat tattcgttat gcaggattag gaatatcaat
     9481 aaaggtcaca tcaagatcgg tattttcatt cagccactcg ctcaatgcgc gaataccacc
     9541 gcgttcagtg gcatggtgac ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg
     9601 aatggtctgt tcagaaactt cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct
     9661 atcgataaaa ctttgcccgc cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc
     9721 ggtatcgcca caccataacg gcttacgtcc cagacgcgct tcaatccagg aggctagttc
     9781 cattcccggc acaggcatgg tcagctctcc ccacggcacc aacggctcaa tttcgcccat
     9841 gaccgtgatc cccagtaacg ccgccagctg tgcgttattg cccagctcag gatgtgcgtc
     9901 aagcggcaaa tgccagccat acaggttgat atcattcgcc agcaacgttt ttaaacggtt
     9961 acgcttcatt ccgcgaatga ccggagactc tcctttccag aagtagccat gatgcacaat
    10021 gaccgcatcc gcgcccagac gcactgcctc atcgagcaaa gcctggctgg cggtgacacc
    10081 ggtaacaatt ttttgcaccg tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc
    10141 actaatcgcc gcgctgttca gtttttcgtt aatcagttgt tccagttcgg tgtttttcat
    10201 cttcgcgcct tccgtattct gatagctaca ccgttaacat agcgttacat cgcctgttcg
    10261 tcgatagcca atcttttgac atattaagaa acaaacccga cgccaaaaga cgcaaattgt
    10321 ttatatcatc acgcttcagc taatgtatat ttatgcaatc agacagcata ttgcataaat
    10381 aatcagatct gtatcaaagt tttaacttta aggaaacaca agcacgccgc gcaactatta
    10441 cgtgacgttg ccgtcaattg tggaattatt atgaataaac acgcatcaca accgcgcgct
    10501 atttactatg tcgttgcgct gcaaatctgg gaatatttta gcttttacgg catgcgtgcc
    10561 ctgctgattc tctatctcac caatcaacta aaatacaacg atactcacgc ctacgagtta
    10621 tttagcgcct actgttcgct ggtgtatgtc acgccaatcc tcggtggctt tttggcggat
    10681 aaagttctcg gcaatcgcat ggcggtgatg ctgggggcgt tgttgatggc gatcggtcat
    10741 gtggtgctgg gtgccagtga gatccatccg tcattcctct atctgtccct ggcgattatc
    10801 gtctgcggct atggcctgtt taaatctaac gtaagctgtc tgctcggcga gctgtatgag
    10861 ccaaccgatc cgcgtcgtga tggcggtttc tcgctgatgt atgcggcggg taacgtgggg
    10921 tctattatcg cacctatcgc ctgtggttac gcccaggaag agtacagctg ggcgatgggc
    10981 tttggcctgg cggcggttgg aatgatcgcg ggtctggtca ttttcttatg tggcaatcgt
    11041 catttcactc atacccgcgg cgttaacaaa aaagtactgc gtgcgacaaa ctttctcctg
    11101 ccgaactggg gatggctgct ggttctgctg gtggcaacgc ctgcactgat taccgtgctg
    11161 ttctggaaag agtggtcggt atacgcctta attgtcgcga ctattattgg cctgggtgta
    11221 ctggcaaaaa tttatcgcaa agcagaaaac cagaaacagc ggaaggagct ggggctgatt
    11281 gtgacgctca ccttcttcag tatgttgttc tgggccttcg cacaacaggg cggtagctcg
    11341 attagccttt atatcgaccg cttcgttaac cgcgatatgt ttggttatac cgttccgacc
    11401 gcgatgttcc agtcgattaa tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg
    11461 gtggttaaag aaagcgtcgc aggtaatcgt accgtgcgca tctggggaaa atttgctctt
    11521 ggccttggcc tgatgagcgc cggattctgc attctgacct taagcgcccg ctggtccgca
    11581 atgtatggtc actcttctct gccactgatg gtgttaggcc tcgcggtgat gggctttgcg
    11641 gaactgttta tcgacccggt tgccatgtcg caaattacgc gcattgaaat tccgggcgtg
    11701 accggcgtat taaccggcat ctacatgctg ctttctggcg cgatcgcgaa ctatctggcg
    11761 ggcgtgattg ccgatcagac atcgcaggct tcgtttgatg cttccggggc gatcaactac
    11821 tccatcaatg catatattga agtatttgat caaattacct ggggcgcact ggcgtgtgta
    11881 ggactggtac tgatgatttg gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg
    11941 ctggagtcat aataggccag caccgatgcc tgatgcgacg cttgacgcgt cttatcaggc
    12001 ctacgtattt cctgcaattt attgaatttg cacaaatttg taggctggat taggcgttca
    12061 cgccgcatcc ggcatctggg ctcgattgcc tgacgcgtct gttatttccc cttccgcgcc
    12121 gcctcatacg ctgccaacgt ttgtactctc gcctctttgt gctcgactat cggttgcgga
    12181 taatccagct tcacacctgc tttctgcgcc cacttccacg gctcatgcac cgctttccct
    12241 ggtacatcgc gcagctccgg taaccatcga cgaataaact cgccctcacg gtcaaatttc
    12301 tcgccctgag ttatcggatt gaaaatacga aaatacggcg cagcatcagt acccgtagaa
    12361 gccgcccact gccagccacc gttattagct gccaaatcac catcaatcag ctgcgacatg
    12421 aaatatcgct cgccttcgcg ccagtcgatc aacaaatctt tcaccagaaa actggctgta
    12481 atcatccgta gccggttatg catccagcca gtgctgttaa gctgacgcat agcggcatca
    12541 ataatcgggt atcccgtttt gcctttctgc caggcctgta aatgtgcggg attgctttgc
    12601 cactgcacac gatccgtcca ggcaataaat ggacgatgtt tacacaacga ggggtaatac
    12661 gtcatcagat gacggtagaa ctcgcgccag atcagctcac taagccagac actaccggcc
    12721 ccaccgtcca gcgcctgcgg ctgttccgcc aacaagcgat gcaaacactg gcgaggcgat
    12781 aacccgcccg ttgccaagct ggcggacaaa cggctggtgc cttccactgc cggaaaatct
    12841 cgttgttgct catactctcc agcaccgttc tcgcaaaact ggcgtaattg cgcaatcgcc
    12901 gctttttctt ccaccgggaa atgcgcagta tcgaaagact gacgaggata attcagcgta
    12961 atggatggcg cgggctttat cgatccgcta ctacgaactt ttggtgcagc gacgcactcc
    13021 ggcatccctt cccgcagccg tttcagccag gcattcttaa aaggcgtaaa gactttgtac
    13081 atctcgtgat tgccggtcat caccgccccc ggcggcagga tcacgctgtc atcaaatcct
    13141 tcacacacca cgttacgcag cgctctttca acgtccacat cccgcgctcg ctcattcacc
    13201 tcatactgat agttataaaa aagatgagta acgctgtttt ccgcgcacac ctgtttaact
    13261 atttcgacac tggcgacaaa gtcatccact tcacggaaca ataaaggaat acctttttcc
    13321 gccagcgcga tttgtagccc attcagttga gcattgataa gttcagcctg acgcggcgac
    13381 atgttatgcg tctcccactt gcgtggtgta gcgatataca gcgccagcac gcgtgcagac
    13441 gaattgcggc aggcggcagc cagtgcgaga ttatcgtgca gacgtaaatc ctggcgaaac
    13501 caggccagat gggtagtcat aaaactcctg aatggcgcaa gttaatcaca cgcggccaaa
    13561 gttcgaaata acgctgccct gaaagccagg cgtcaggata aagcgccata tagtgtttga
    13621 tacgcatcag cggcgcaaga agtggttgct cgccacggcg ataactgtcg ataagcgcag
    13681 ccagctgctg gcgttctgtg gaatcaatat gcgggcgaaa ataaccctga acgtgcatca
    13741 aaacgttggt gtgatcgcgc gggttggcgg gatgagaaag caaaacaatt acgcgttggc
    13801 ggtactggtt gtaaaaatca tccagatttt gccactgatg aatatcagcc acaaacgggc
    13861 caagtttgcg gtactcgggc tgagaatggg ccagaaagac gagtttatag cggctgtgat
    13921 aatcgagaag cgccccgcgc gttaagccat gttctttcag tcgatgtaac tcatgaaggg
    13981 cgaagatacg gattagggtg ctgtcatcaa atcgttgtag attcattact gctcctgaac
    14041 ggtatacagg agtaagtgta ggcgttggta aatgatgttt gtgaagttaa tccgctagca
    14101 gggcaagatt acgaaagcgc gctccccgca agcaactaat caaggtagtt aatcatggct
    14161 gaacacgata ttacgccaga agtcactatt tccaaaacac agcgccgcta taaagtcggc
    14221 tatgtcagag cccgccacca ggaccgcaaa accggtatag cccggtatta ttcacaacat
    14281 cccagtctgc accttaaggg cgactggctg aaagaggccg gatttgatac cggacggggt
    14341 gtcacggtga agatttcgga ggggggcctg accatcatcg ctgacagtga cgaggtgcag
    14401 gaactgcggg atgggatgtt tagtgtgctg aacgaaagtt aaatattaaa aaagctggga
    14461 agatttaaaa ccttcctagc tttttattct gtcttattat aatggatact catcatcttc
    14521 cgatgaattt ataaatt
//
LOCUS       DAZPEU010000093        14470 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPEU010000093 DAZPEU010000000
VERSION     DAZPEU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.094"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..171)
                     /locus_tag="ACHU2L_002510"
     CDS             complement(<1..171)
                     /locus_tag="ACHU2L_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR1357335.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMTPTY"
     gene            complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHU2L_002511"
     CDS             complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHU2L_002511"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1357336.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHU2L_002512"
     CDS             complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHU2L_002512"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR1357337.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHU2L_002513"
     CDS             complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHU2L_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR1357338.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2840..3175)
                     /locus_tag="ACHU2L_002514"
     CDS             complement(2840..3175)
                     /locus_tag="ACHU2L_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR1357339.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            3577..5805
                     /gene="crfC"
                     /locus_tag="ACHU2L_002515"
     CDS             3577..5805
                     /gene="crfC"
                     /locus_tag="ACHU2L_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR1357340.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            5802..6680
                     /locus_tag="ACHU2L_002516"
     CDS             5802..6680
                     /locus_tag="ACHU2L_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFR1357341.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            6944..8446
                     /gene="proP"
                     /locus_tag="ACHU2L_002517"
     CDS             6944..8446
                     /gene="proP"
                     /locus_tag="ACHU2L_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR1357342.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            8558..8647
                     /gene="pmrR"
                     /locus_tag="ACHU2L_002518"
     CDS             8558..8647
                     /gene="pmrR"
                     /locus_tag="ACHU2L_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR1357343.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8623..9723)
                     /gene="pmrB"
                     /locus_tag="ACHU2L_002519"
     CDS             complement(8623..9723)
                     /gene="pmrB"
                     /locus_tag="ACHU2L_002519"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR1357344.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(9724..10392)
                     /gene="basR"
                     /locus_tag="ACHU2L_002520"
     CDS             complement(9724..10392)
                     /gene="basR"
                     /locus_tag="ACHU2L_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR1357345.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(10389..12032)
                     /gene="eptA"
                     /locus_tag="ACHU2L_002521"
     CDS             complement(10389..12032)
                     /gene="eptA"
                     /locus_tag="ACHU2L_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR1357346.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(12136..13473)
                     /gene="adiC"
                     /locus_tag="ACHU2L_002522"
     CDS             complement(12136..13473)
                     /gene="adiC"
                     /locus_tag="ACHU2L_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR1357347.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(13610..14371)
                     /gene="adiY"
                     /locus_tag="ACHU2L_002523"
     CDS             complement(13610..14371)
                     /gene="adiY"
                     /locus_tag="ACHU2L_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR1357348.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
BASE COUNT         3432 a         3568 c         3819 g         3651 t
ORIGIN      
        1 gtaagttgga gtcattaccg cgaaggttgc catgttgccg ccgtctttaa tcagcgtaag
       61 cggggccatt tccgcgccct gccacgagac gaccagcacg tcgagaacaa cggcccagct
      121 cagaagcgag aaccagctac gcttgggtgg ggcgatggtg atggtttgca tgtttggctc
      181 cggtctgtag gccggataag gtgcttgcac cgcatccggc atcaacgcct acacattgtc
      241 ggatgcgacg ccggggcgtc ttatccgacc tacgatttac tgcaccgctt tactcaccga
      301 actcatcgcg cttaacgcgt tgttcaggcg gtccaggtca tccagctgcg cctgaatctc
      361 ggtggttttc gccagcttgt cctgctcatt cagcccttta ttgcctttca cgctttgtat
      421 ctctttaaac agtgcgagct ggcgaatcgg caccagttgc aggtcgctgg aagcgcggaa
      481 cggcgcccag cccaggcgtt ccagcaccgc tttctcttcc ggcgtttttc cgtagttcat
      541 aaagaagtcg tagatcttgt ctttggtagt ttcggacaga ttcttgcgcc agacgatcgg
      601 atcgcctagg atcagcggcg atttccagat caccttcagc tctttaagct tctccggcgc
      661 ggaggttttc agcttgtcga ggttttcggt gttgttggtg gcaacatcca cttgcttgtt
      721 ggcgacggcc agcgcgttgg tttcatgccc ggcgttgacg gtgcgcttga agtcgctggc
      781 ggagatattg tttttagcga agacgtagta accggggacg aggaagccag aggtggagtt
      841 aggatcgcca ttgccgaagg tgagatcttt ccgcttcgcc agcagatcgt tcaggttgtt
      901 gatcgggctg tctttgttga cgatcaacac gctccagtaa cccggcgatc catccgccgc
      961 gaccgtctgg gcgaagacct ggccattggc gcgatccacc gcttccatcg ccgacaggtt
     1021 gccgtaccag gcaatatcca ctttattgaa gcgcatcccc tggataatgc ccgcgtagtc
     1081 cggggcaaag aaggcgttga ccttcacgcc cagtttcttc tccatatcct gtaaaaacgg
     1141 cgtccattgc ggtttcaggt tttgctgtga ttccgttgaa ataatgccga aattcagcgc
     1201 cttttcctgc tcttcggcgt gtgccggggt taacagggtg ctgaggctga acatgctggt
     1261 gaaggccagc gaggcaatta tcttagcgtt cattcgtttt cctcaatgat ggggatatca
     1321 ggcagctttc gcgttctctt cgatgcggtt aatgctgcgg tagagatggt caaaacgttc
     1381 gttatcaaac agttggctgc tgccgtcgta gaagacgtgc ccctgacgca gggcgacgat
     1441 gcgttcgcag tagcgcaggg cgtaatccac ctgatgcagc gtgacgacca cggtgatgcc
     1501 gtcgttctgg ttgatgtcgc gcagggtgtc catcacgatg cgcgctgatt ccgggtccag
     1561 cgaggcgatg ggttcatcgg ccagaatcac tttcgcctgc tgcatcagcg cacgggcaat
     1621 cgccacacgt tgctgctggc cgccggagag ggtggaaacg cgctgatggg caaaatgcac
     1681 catgccaacg cgggtcagcg cctgtaacgc gcgctgtttc tgctcgccgg tgaaccagct
     1741 aaaacaggtg cgccagaacg gcgtgctgcc gagcgcgcca atcagcacgt tctccagtac
     1801 gctcaggcgg ttcaccaggt tgaattgttg aaagatgtag ccggtgttgg cgcggctttt
     1861 gcggatatcg cgggccaggc ggccttcgcg ctggacagtg cggcccagta gctcgatatg
     1921 gctaccggcg gatttatcgc cggtaatcaa accgcttaaa tgacgtaaaa gggtggattt
     1981 gccggaaccc gacggcccaa gcagagccac catttcactg tgatgaatgt tcagatcaac
     2041 cgcatgcagc gcttgatgct gattgaaggt tttggcgagc ttctcgacac ggataatcgt
     2101 ttgcatgatg cagcctccct aaaaaagtgg ctccatcgtg gcggattatt gtgacgattt
     2161 ggttaattaa aagtgactaa cagatgaaga gttaacggga attcgatgac agtgcgggag
     2221 ggcgggcctc ccggcgggtt attgcgttgg ttgttgtttg acgacattaa tcatccacgg
     2281 tacgccaaat ttatcggtga ctttgccaaa gccatgcgcc cagaaagttt cctgccaggc
     2341 catttcgatt tttccgttag cggcaaggtt atcaaaccag cgttttcctt cttcgacctg
     2401 ttgcgaatcg agcaccagcg taaagccgga gtagctggct tttcctgacg gtatagcatc
     2461 gctcatcatg atgtcgcttc cggcaatgcg cacgttggca tgagcgatgg cggtatcggg
     2521 aaattgcatt ccggaagggc agttctcggc gctgtcctgc gctgatttcg gcatttcgcc
     2581 gaagctgatt ttatagagca gttccgcgcc caacgtacgt tgataatagg caatcgcgtc
     2641 ggaacagtta ccggcaaaag agaggtaggg acttaacggc atgatggtga cctcagttaa
     2701 gagaagccag ttaagtgtag ttcgaaattt atacagatga gaggctgcct gataagacgc
     2761 gcaagcgtcg catcaggcag tcggcatcgt tgccggatgc ggcgcgagcg ccttatccgg
     2821 cttaccgatc acaatacaat cagttctttt tcacaaactc agatttcagt ttcatcggac
     2881 caaaaccgtc gattttgcaa tcgatgttat ggtcgccttc aaccaggcgg atgtttttca
     2941 ctttggtgcc aattttcagc atcgaagagc tacctttcac cttcagatct ttaatgatgg
     3001 taacgctgtc gccgtcagcc agcagattgc cgttagcatc tttaacgatc agctcgtcgc
     3061 tttcctgtgc aggttctgcg tcgttccatt cgtaggcaca ttccgggcag atgtacatgc
     3121 cgttatcttc gtaagtgtat tcggagttgc attttgggca gtgtggtaat gacatgtgga
     3181 tttcctcaaa agtcagcagg gcaaaagcgc caaaaaacgg cagattgccg aaaaaggccg
     3241 caattataca caaaatccct gagtttgtcg ggggtactgc tgattttaga cgacgatcgt
     3301 tttcatttga aaggaaacgc aaaaagaaaa taatcttttt gcctctattt attatttaag
     3361 ataatgttaa tgctctactt gttctttttt tattcacatg atatttttat ctttctgacg
     3421 ttttaaacgc atcagaagac gttatttatt ctgataaaaa aacaacacac taaatataaa
     3481 attatttctt gcagtaatta taaaagccga aaacagaact caaaaacaat ctggctaaat
     3541 aaaagtaaca aaatttgctt taaggaagaa ttttctatgt acacacagac cctgtatgag
     3601 ttaagtcagg aggctgaacg cctgttacag ctttctcgcc aacagttgca gttactggaa
     3661 aaaatgcctc tctctgtacc cggagacgac gcgccacaac tggctttacc ctggagtcag
     3721 cctaatatcg ccgaacgtca cgcgatgctg aataatgagt tgtgtaaaat ttcccgactg
     3781 gaaatggtgc tggcaattgt cggtaccatg aaagcaggga aatcaaccac cattaatgcc
     3841 attgttggta cggaggttct gcctaatcgt aatcgcccaa tgactgcgct gccgacgctt
     3901 attcgccata cgcccgggca aaaggaaccg gtactgcatt tttcacatgt cgcgccaatc
     3961 gattgtttaa ttcaacaatt acaacagcgc ctgcgtgatt gcgatattaa gcatctgacc
     4021 gatgtgctgg aaatagataa agatatgcgt gcgcttatgc agcggatcga aaatggcgtc
     4081 gctttcgaaa aatattatct gggtgcccag cctatttttc attgtctgaa aagtttgaat
     4141 gatttagtgc gactggcgaa ggcgctggac gtcgattttc ctttttctgc ttacgccgcc
     4201 attgagcata ttcccgtgat tgaagtggag tttgtccatc tggcggggct ggaaagttat
     4261 cccggtcagt tgacgttact ggataccccc gggccaaatg aagccgggca accgcatctg
     4321 caaaaaatgc ttaaccagca gctggcacgc gcctcggcgg tactggcggt gctggattat
     4381 acgcaactga aatcgatctc cgatgaagag gtccgtgagg cgattttggc ggtggggcaa
     4441 tcggtgccgc tgtatgtgct ggtcaataag ttcgatcaac aggatcgtaa cagtgacgac
     4501 gccgaccagg tgcgggcact gatttccggg acgctgatga aaggctgtat tacgccacag
     4561 cagatattcc cggtgtcgtc gatgtggggc tacctggcga atcgggcgcg ctatgagtta
     4621 gccaacaacg gtaagttacc agcgccagag caacaacgct gggtggaaga ttttgcccat
     4681 gccgcgctcg gcaggcgctg gcgtcatgcc gatctggcgg acctcgaaca tattcgtcat
     4741 gctgccgatc agttgtggga agattcgctg ttcgcccagc caattcaggc gttgcttcat
     4801 gccgcttacg ctaacgcctc attgtatgct ctgcgatctg ctgcgcataa actgttgaat
     4861 tacgcgcagc aggcgcggga atacctggat tttcgtgcgc acgggttaaa cgtcgcttgt
     4921 gaacaattgc ggcaaaatat ccatcaggtc gaagaaagtt tgcagctatt gcaactcaat
     4981 caggcgcagg tgagcggcga gattaaacat gaaatcgagc tggccctgac ctccgccaac
     5041 cactttctgc gtcaacagca agatgcggtg aatgcgcagt tagccgcctt gtttcaggat
     5101 gattcggagc cgttaagcga gattcgtacc cgctgtgaga cactgttaca gacggcgcag
     5161 aacaccatca gtcgcgactt tacgctgcgt tttgccgaga tggaatccac cctttgccgg
     5221 gtgttaaccg atgttattcg ccccattgag caacaagtca aaatggaatt gagcgagtca
     5281 gggtttcgtc ctgggtttca ttttcctgtt tttcacggcg tagttcccca cttcaacact
     5341 cgccagctgt tcagtgaagt catttcgcgc caggaagcaa cggacgagca gagcacgcgt
     5401 ttaggcgttg tgcgtgagac tttttcgcgc tggttgaatc agcccgactg gggacgggga
     5461 aatgagaaat ccccgacaga aacggttgat tacagtgtgt tgcaacgagc attaagcgca
     5521 gaagtcgatc tctattgcca acaaatggct aaagttctgg cagagcaggt cgatgaatct
     5581 gttacggcag gcatgaatac ttttttcgct gagttcgctt catgtttgac agaattacag
     5641 acgcgtttac gcgaaagtct ggctctgcgt caacaaaatg aatcggtggt caggctgatg
     5701 cagcagcaat tgcagcagac tgtgatgact cacagctgga tttacaccga cgctcagctg
     5761 ttacgcgatg atattcaaac acttttcacg gcagaacgat attgaccaag acgttactgg
     5821 acggccccgg tcgcgtgctg gagtcggttc atccgcgctt tttagtggat ctggcgcagg
     5881 gcgatgatgc ccgccatcct caagcccatc agcagcagtt tcgtgaacga ctgatgcagg
     5941 aacttctttc gcgtgtgcag cttcagacat ggacgaacgg cggcatgtta aatgcgccgc
     6001 ttagcctgcg actgacaatg gtggaaaaac tggcgtcgat gctggatccc ggtcatctgg
     6061 cgctgacgca gatcgcgcag catctggcgc tgctgcaaaa aatggatcac cgccagcact
     6121 ctgctttccc ggagctcccc cagcaaattg ccgccttgta tgagtggttt tcagcccgtt
     6181 gtcgctggaa ggaaaaggcg ttaacgcaac gaggcctact ggtgcaggca ggtgatcaga
     6241 gcgagcaaat ttttacccgc tggcgtgctg gggcgtataa cgcctggtcg ttgcctgggc
     6301 gctgttttat cgttctggag gagttgcgct ggggtgcatt tggcgatgcc tgccgtctgg
     6361 gaagcccgca agcggtggtg ttgttgctgg gtgatttgcg cgagaaagcg acacaacatc
     6421 tggcagagag tatcaatgcg gcaccgacta cgcgtcacta ttaccatcag tggtttgcct
     6481 cttcgaccgt tccgacgggc ggggatcatg ctgatttttt aagttggctg ggaaagtgga
     6541 ccacggcaga taaacaaccc gtttgctggt cagtgaccca acgctggcaa actgtcgcgc
     6601 tggggatgcc acgactctgt tcagcgcagc gtttggtggg ggcaatggtc gaggaaatct
     6661 tctctgtaaa tttggcgtaa ataatcagtt acatcaatga gtcctaaacg aaatccatgt
     6721 gtgaagttga tcacaaattt aaacactggt agggtaaaaa ggtcattaac tgcccaattc
     6781 aggcgtcaac tggtttgatt gtacattcct taaccggagg gtgtaagcaa acccgctacg
     6841 cttgttacag agattgcatc ctgcaattcc cgctcccctt ttgcggccgt cgcgctgatt
     6901 tttctggcgt ttgcggaaat gggccaactc tgcgaggaaa gctatgctga aaaggaaaaa
     6961 agtaaaaccg attacccttc gtgatgtcac cattattgat gacggtaaac tgcgtaaagc
     7021 cattaccgca gcatcactgg gtaatgcaat ggaatggttt gattttggtg tttatggttt
     7081 tgttgcttac gcattaggta aagttttttt cccgggggct gaccccagcg tgcagatggt
     7141 tgctgcactt gccactttct ccgttccctt tctgattcga ccgcttggcg gactcttctt
     7201 tggtatgttg ggcgataaat atggtcgcca gaagatcctc gctatcacta ttgtgattat
     7261 gtcgatcagt acgttctgta ttggcttaat accgtcctac gacacgattg gtatttgggc
     7321 accgattctg ctgttgatct gtaagatggc acaaggtttc tcggtcggtg gtgaatatac
     7381 cggggcgtcg atatttgttg cggaatactc ccctgaccgt aaacgtggct ttatgggcag
     7441 ctggctggac ttcggttcta ttgccgggtt tgtgctgggt gcgggcgtgg tggtgttaat
     7501 ttcgaccatt gtcggcgaag cgaacttcct cgactggggc tggcgtattc cgttctttat
     7561 tgctctgccg ttagggatta tcgggcttta cctgcgccat gcgctggaag aaactccggc
     7621 gttccagcag catgttgata aactggaaca gggcgaccgc gaaggtttgc aggatggccc
     7681 gaaagtctcg tttaaagaga ttgccactaa atactggcgc agcctgttga catgtattgg
     7741 tctggtaatt gccaccaacg tgacttacta catgttgctg acctatatgc cgagttattt
     7801 gtcgcataac ctgcattact ccgaagacca cggggtgctg attattatcg ccattatgat
     7861 cggtatgctg tttgtccagc cggtgatggg cttgctgagt gaccgttttg gccgtcgtcc
     7921 gtttgtgcta cttggtagtg ttgcactgtt tgtgttggcg atcccggcgt ttattctgat
     7981 taacagtaac gtcatcggcc tgatttttgc cgggttactg atgctggcgg tgatccttaa
     8041 ctgctttatg ggcgttatgg cttctacctt gccagcgatg ttcccgacgc atatccgtta
     8101 cagcgcgctg gcggcggcat ttaatatttc ggtgctggtt gccggtctga cgccaacact
     8161 ggcggcctgg ctggtcgaaa gctcgcagaa tctgatgatg cctgcctatt acctgatggt
     8221 agtggcggtg attggtttaa tcaccggcgt aaccatgaaa gagacggcaa atcgtccgtt
     8281 gaaaggtgcg acaccggcgg cgtcagatat acaggaagcg aaggaaattc tcgtcgagca
     8341 ttacgataat atcgagcaga aaatcgataa tattgaccac gagattgccg atttgcaggc
     8401 gaaacgtacc cgcctggtgc agcaacatcc gcgaattgat gaataagctg aaacggatgg
     8461 cctgatgtga cgctgtctta tcaggccaat tgaactctta aggttcactt aatctctgac
     8521 gcgcatactc tcctccaggt taacggagga gagtgcaatg aaaaaccgtg tttatgaaag
     8581 tttaactacc gtgttcagcg tgctggtggt cagcagcttt ctttatatct ggtttgccac
     8641 gttctgatct ttcttcagcc gtacccaggc ccgcgtgccg gaagtctctt gccggttttg
     8701 caggaaaaac tgcccgtgat gcaactgtgt aatgcggctg acaatactta accccagacc
     8761 aatcccgcca taacggctgt ccatacgtac aaacgcttta ctcaactccc cgcatttact
     8821 ctcatcaata cctggtcctt catcttcaac tgccatgacc gctccgccat cttcttgcag
     8881 cttaatcata atgttgctgc cttgcgggct gtaacgatgg gcgttttcta ccaggtttcg
     8941 caataacatc cgcagcaggg ttgcatcacc ctgaacggtg atgtcggcgg cgctctctgg
     9001 caatagcagg gtttgctgtc gctggtcgag catggtactg agttcgtcat acgaggggag
     9061 aatgacatct tccagcagtt ttacatgttg ataattaccg gaagaaaatg actgtccggc
     9121 acgcgccagt tgcagcagct gggagacgct ctccatcatc tgatcaagcc gtgccactaa
     9181 cggtgctaca tcaatgtgat gcgttttcgc cagcagttcc agatgcaaac gcacccccgc
     9241 cagtggcgtt cgcagttcgt gcgcgacgtc agcggtaaac aacctttcgt tatccagcgt
     9301 gctggtcagg cgactgacca gatcgttcaa cgccgaaacc accgcttcga tttcgagggt
     9361 ggcgctgtga atggcaatgg gcgttaagtt gtcggcggtg cgcgcttcca gctctttttg
     9421 cagctccgcc agcgggcggg tgatgcggcg taccgcctga tagcagataa atagcgtcag
     9481 gctgaccata aagacgccgg ggacaatcag gctggcgacc gcctcgcgga tctcacgcat
     9541 gatgtggcga tcgttgttgc gattgtcgcg tagcgcctgc tcaaacagct gaatctgctc
     9601 ggtactttca tgccatagcc agaagacgct gatcagctca aacaccaaca aaatggcccc
     9661 gatggtcaat atcagccgtt ggcgcagcga tataggtcgg cgcagaaaac gcatcagatt
     9721 caattagttt tcctcattcg cgaccagcat atagccaaag ccgcgcacgg tgcggatacg
     9781 ggctttgccc actttgtcgc gcagattgtg gatatgcact tccagggtgt tggtcgaggg
     9841 ttcattgtcc cagttataga tgtcgttgta gagaatttcc cgatgtaccg gactgcctgc
     9901 tttgagcatt aaccgtgaca gcagagcata ttctttgggc gtcagaatca actcttcacc
     9961 gcccatccat acctgacggc gacccatatt cagcgtcaga ttgccaacaa tcagctcact
    10021 ttcgccctga ttattatggc gtcgtagcag ggcgcggata cgggcgtgta actcttccag
    10081 cgcaaaaggc ttcaccagat agtcatcggc accgacatcc agcccggcga ttttgtcggt
    10141 cagcgtatcg cgagcggtga ggatcagtac cggcagggtg tattttttct gccggatacg
    10201 ggcgagaaaa tgcagtccat cttcgtcggg taaccctaaa tccagtacca ccaggctgta
    10261 atgaccgtcc tcaaggcttt gttccgccat ccgcgcggtt gtcacgccat cgcacgcgta
    10321 gccttcggtt tgcgccgcca gaatcagtcc ctgcaataac agcgtatcgt cttcaacaat
    10381 cagaattttc atttactcac tctcctgcaa gtttgcagaa tatcatccgc ggcctggtaa
    10441 tacttcgtct caacgccagt taatcccaat agcgtggaga ataaattgtc ttgtgaatag
    10501 tgttgcgttt gcgcctgttt ttgcaggcag ttctggtcaa cctgataccg cttttgataa
    10561 tcctccgaca gccacagcag cattggcacc tgtttttggc tatccggggc gatggcataa
    10621 ggcagaccgt gcagatagat gccattttca cctaacgatt caccgtggtc agaaagataa
    10681 accaggctgg tggtaaattt atcctgatgt tctttaagca gattaatcgc tttatcaaca
    10741 atatagtcga cgtaaaccag cgtgttgtcg taagtgttca ccagttgctc tttggtacag
    10801 gtctggatct cattggtgtc gcaggttggg gtaaatttcc tgaactgcgg cggatagcgg
    10861 ttgtaatagg tcggaccgtg gctgccgatg gtgtgtaaga caatcacgcc atcaccctgc
    10921 aggttattga tgtactcttc cagcccgtgg aacagcactt cgtcatagca ttcgccgttg
    10981 atgcactgat caggcagatt cagcgcggtg acgttctggt gaggcacgcg gtcgcaggca
    11041 cctttacagc cgccatcgtt gtcattccac agcacgttga tgcccgctcg ctgaatgata
    11101 tccagcacgc cttcctggtg ctgtgccagc tcttctttgt agtgctcacg cggcatatcc
    11161 gagaacatgc acggtactga aactgccgtt gccgtgccgc aagatgcggt attagggaaa
    11221 tagaccacgt tatctttcgc cagccgcggg ttagtttcac gcgggtagcc gttgagggag
    11281 aagttctccg cccgcgaggt ttcgccgaca atcaggatgg tcaaattttt acgtttttcg
    11341 ttctgcatta acgggttgcg gtgcgcgtct tcaccaattc gcaccagcgg cagatttgcc
    11401 agtcgctgat gggagtacca tgaccagctg gcaacaatgc tgttagaggg gcttaaggat
    11461 ttcaccagct ctttgttatt gcggaacaac gaggcgtagt ctttataaaa cagtgcggcg
    11521 accagcaaaa tcagcagtac agaaaccaga atattggctc cacggaaaag aacactgcgc
    11581 agacgcgagg tggcaggttt gatttttagc cagcaggcaa tcagctcagc aagcacgccg
    11641 ctgaatccca gcgttaataa catttgcggt gtcatcagcg cataactttc tgctggagtg
    11701 gtatcaataa tattggcaat catcgagcgg tcgatgacga tgccgtaagt cattatgaaa
    11761 tattgtgcag ccgcgccaat cagaataaac aggcaggcca gtggtcgatt aagccataag
    11821 aaagagctta gtgtcaggac aatattaatc acgctgaaag cgacaaccgg catcgacaag
    11881 aaaaccagta cgttatgcag cgaatccagc ggcagcgcct gcaacacctg tttaaaaaag
    11941 gcaatattca ggcagataga gatataaaaa gcggccaaca atagccaggc gagtaaattc
    12001 aaagagggtc tttttagtag gcgcttcaac atcacggtgt ttccatcgaa caaagtgcgc
    12061 atatgctcgc aaagcaaaat taagccaacc ttaagttctt aaggttggct tttatgtttg
    12121 ctggattaag gggaattaat ctttgcttat tggtgcatct aagggatacg ggtttttatg
    12181 tagccggttg taattcagcg catacatggc ggtgatgacc atcagggtga caaatgacca
    12241 cataacctct ttcgccccgg accccaccac ggcccaaatg cagtagagga aggcaatggt
    12301 agtaacagcc agatatgccg ggcgtgcttt accaaagtga ccgtggccga gcagcagtaa
    12361 cgccgcacag gtgtaaagat atggcaccag tgtaaagatg accgagacgg aagaaaccag
    12421 accgaactct ttggtcgcgt ttggtgaaat gctgctgagc tggaagatgg tcatcaaaat
    12481 accgacgata atcagccccg ccactggcgt acccgcttta tttacacggg caaaaatcgg
    12541 tgggaacagt ccgtcatcgg cagcggcttt cgccgtttga cccgccagca acgtccagcc
    12601 gcccagtgaa cctaagcaac ccgcagctgc gcagaaggaa acaatggccc cagcggtgtc
    12661 acccaacgcc atccgtgcgg catcaccgaa tggcgaggca gaaacgcgca gtgcggcatt
    12721 agggatcatc cccataatcg cggtggtgga gagcacatag caaacggcgg caatcaatac
    12781 cccaccaatg gtggcgatag ggacattgcg tttcgggttt ttcaccacac ctgcggcaac
    12841 ggaggcactt tccacaccga tgaacgacca cagcgtaacg ttaagggtac tttgaatcgc
    12901 accgaaggtg cccaggccgc tgacgttcca tgccgccata taggtttcac cacggaacca
    12961 gaaccagcca aatacggcaa tcccgacgat ggggatcagc gccagcacag tggcaacggc
    13021 ctgcacgcgg gtgatcattt tcggaccgac aatgttcagc aggacgaaga tccacagcac
    13081 cacgacgcag gtgatggtta ataccaatgg atctttcaga atcgggaaga agtaacttaa
    13141 atatcctacg ccaatgacca ccatggcgat attgccgatc cagcaggcca gccagtagag
    13201 gacgttggtt tgataaccga gaaacgggcc aaagcagcgg cgggcgtaag cgtaagaacc
    13261 accaggactt gggtcgagga acgacatttt ggcgtatacc atcgagagcc ccagcgcacc
    13321 gataatcgtc accaaccatc cataaatggc aatcccgcca gtggaggcca ggtttgcagg
    13381 taacagaaaa acacctgacc ccataatatt ccccgacacc atcagggtga cggggattaa
    13441 gcccactttg tgagcatcag catccgaaga cataattaaa ctcctgcgaa ggcgagcttc
    13501 gtgacaataa attacgtcat atcatacgcc tgcattcgat agttgaatta ttatcgaccg
    13561 ggttatcgct gatgccgtta ttaatatagc tcttcgttaa ggttaaaaat caggcgacag
    13621 tgcgttctct gtgctgacta acataatgca gcggcgtcat accgtagaag tctttaaaga
    13681 cagaaataaa gtacgacgta ctgttgtagc cgcaggactg tgatacctga gagatatttt
    13741 taccgtccat cattaattca tttacggcat aacgcatgcg gcaggtggtg attatttggc
    13801 tataactggt gttttcgctt ttcaactttt tctttaacag acttgggcta agacataaac
    13861 aactggctac catactaaga ttccagtctt tgtgaatatc gctttcaata atttgataaa
    13921 cgctgtcact tacacaatta cgtaacatat acatcattaa tgaaaggaat tttttactgt
    13981 cgagaaaacg ggatagcacc gtgaataata atgcgcgtgt tcgttccttt tctgactcag
    14041 tttctgcggg catcatgctg tgttgcgcgg caacgcgaaa gacgtctggc gtcaggcatg
    14101 gcagggtgag tatgggcgta gcgctacgtt gccatactgg tatttgcgag agatctttat
    14161 tcagaaaccg gaggtaatct ttgatgatgt cgtgactaat atgagcaacc aaagtgttgt
    14221 taagagagga gacgtcgata atattatttt cacaatttaa taacgccata tggttagctt
    14281 taaggctaat aggttctttc ccattcaccc ttatccagac atctttttca gtcaataaaa
    14341 caatacaagg ttggtcgctg caaatcctca tacatgtact cctgagtgcg aataaaagac
    14401 cagttctcgc taaagcaaag cgataccggg aaatggttat ataattgcat gatgaatata
    14461 aacatgcata
//
LOCUS       DAZPEU010000094        14423 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPEU010000094 DAZPEU010000000
VERSION     DAZPEU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.095"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..185
                     /locus_tag="ACHU2L_002524"
     CDS             <1..185
                     /locus_tag="ACHU2L_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1357349.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="ACHU2L_002525"
     CDS             complement(210..401)
                     /locus_tag="ACHU2L_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR1357350.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="ACHU2L_002526"
     CDS             complement(506..742)
                     /locus_tag="ACHU2L_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR1357351.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACHU2L_002527"
                     /pseudo
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACHU2L_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACHU2L_002528"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACHU2L_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACHU2L_002529"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACHU2L_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR1357352.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="ACHU2L_002530"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="ACHU2L_002530"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR1357353.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="ACHU2L_002531"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="ACHU2L_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR1357354.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="ACHU2L_002532"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="ACHU2L_002532"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR1357355.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="ACHU2L_002533"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="ACHU2L_002533"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR1357356.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMMMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="ACHU2L_002534"
     CDS             complement(10860..12233)
                     /locus_tag="ACHU2L_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1357357.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="ACHU2L_002535"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="ACHU2L_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR1357358.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="ACHU2L_002536"
     CDS             complement(13344..13583)
                     /locus_tag="ACHU2L_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR1357359.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="ACHU2L_002537"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="ACHU2L_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR1357360.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="ACHU2L_002538"
     CDS             13960..14247
                     /locus_tag="ACHU2L_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR1357361.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3789 a         3520 c         3695 g         3419 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtat
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcatggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atgatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa
    14401 aagatatttt cctcattaat tta
//
LOCUS       DAZPEU010000095        14318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPEU010000095 DAZPEU010000000
VERSION     DAZPEU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.096"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..3052
                     /locus_tag="ACHU2L_002539"
                     /pseudo
     CDS             1..3052
                     /locus_tag="ACHU2L_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3062..6708
                     /locus_tag="ACHU2L_002540"
                     /pseudo
     CDS             3062..6708
                     /locus_tag="ACHU2L_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6769..7176
                     /locus_tag="ACHU2L_002541"
     CDS             6769..7176
                     /locus_tag="ACHU2L_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357362.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7718..8806
                     /locus_tag="ACHU2L_002542"
     CDS             7718..8806
                     /locus_tag="ACHU2L_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR1357363.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8817..11096
                     /locus_tag="ACHU2L_002543"
     CDS             8817..11096
                     /locus_tag="ACHU2L_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR1357364.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11089..12225
                     /locus_tag="ACHU2L_002544"
     CDS             11089..12225
                     /locus_tag="ACHU2L_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1357365.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12222..14225
                     /locus_tag="ACHU2L_002545"
     CDS             12222..14225
                     /locus_tag="ACHU2L_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357366.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3546 a         3755 c         3654 g         3363 t
ORIGIN      
        1 tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg
       61 gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct
      121 ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg
      181 caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag
      241 catctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat
      301 gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac
      361 tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc
      421 cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat
      481 ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga
      541 atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta
      601 gtgcaaaaag aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca
      661 gcactggcag aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca
      721 tgccagcacc aactgacaca gccctcaaac catgccagcg ccgatctact gccagccata
      781 gtagtctccc caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta
      841 gcgccattag gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg
      901 gcgaaatata tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac
      961 ctgttggcaa ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa
     1021 gcggtagttg aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg
     1081 tttcattcac ctaccgggca ttattggcag ttggggattt tgacaacatt gccgctggag
     1141 gctggagaaa gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga
     1201 atacgccatg ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg
     1261 ctatccacag gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
     1321 cgtcgcccgt gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt
     1381 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
     1441 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
     1501 actgcaagaa atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct
     1561 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta
     1621 tcagccatcg atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa
     1681 cgccctcctc cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     1741 attattgcag gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctgggatct
     1801 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     1861 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     1921 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag
     1981 tgatatcgcc ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca
     2041 ggagcgggca aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga
     2101 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga
     2161 ttttggccca cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     2221 tgtttccggc agccgcctga aagacctgcc aaaaccaaac aaaagcgatt atgagtcaca
     2281 ggcgaacgat gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgcggc
     2341 ccagcaggta gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa
     2401 cttccaactc ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg
     2461 gggcgtttat agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag
     2521 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat
     2581 tcctcacgtt ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga
     2641 ctacgaactg ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc
     2701 cgagcgcaac gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt
     2761 catggggctg gcaaataaag gctggataaa gggcgaacca caggatggag gctggatgat
     2821 caaacctctc ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat
     2881 atcaccagcc gaacttagcg ctgagcagct cttaagcaag ctgtggctat gggaaggcaa
     2941 agcggaaagc tatggctggg ggagtaattc aacacaggaa gcgcagttct ccgtactcga
     3001 tgccatcacc gccagcgagc taattaacga tattgaagcc ctgtttgaat aaggaaagag
     3061 catggacaag gaattaccgt ggctggcgga taacgcccaa ctggaactga aatataaaaa
     3121 aggcaaaaca ccactcagtc atcgcaattg gccgggcgaa ccagtgcctg ttatcactga
     3181 aagcatcatt cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat
     3241 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
     3301 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
     3361 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctggaata
     3421 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
     3481 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
     3541 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
     3601 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
     3661 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
     3721 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gttgctactg accccaaagc
     3781 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     3841 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     3901 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     3961 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     4021 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     4081 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     4141 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     4201 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     4261 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     4321 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     4381 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     4441 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     4501 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     4561 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     4621 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     4681 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     4741 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     4801 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     4861 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     4921 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     4981 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     5041 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     5101 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     5161 acttgccctg cgtttacttt acgaacatgg gcatggcgaa tggctacaaa ctgtagccaa
     5221 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     5281 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     5341 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     5401 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     5461 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     5521 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     5581 aagcacggca cgggatctaa cgacacatat cctcgcctgg ccacaagaag gcaaatctgc
     5641 ccgtgcggtc agtggcatga acatccttac cctgatgaat aatgatatgg cgctgatgca
     5701 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     5761 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     5821 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     5881 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     5941 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     6001 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     6061 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     6121 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     6181 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     6241 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     6301 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     6361 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     6421 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     6481 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     6541 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     6601 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     6661 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     6721 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     6781 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     6841 agacttgaga tccataaatg ctctgaatta tttacaggtt ccgtctaccg actggaacgg
     6901 ttccggctac gtccaacatt tcatcaacgt gatcgagagg atgttgaccc gctaataaat
     6961 gatgcgttga tttatataag agatgagttt attgatgagc aaaaattacg aggcgaatca
     7021 cctgaacctg taatagtaat ttttaattgt aaactacaga atatattcaa ccaacaaata
     7081 gaattatata ctctagataa ttcaagtcgc agcacatgca acttgaagta tgacgagtat
     7141 agctttatta aaggggggaa cggtattccc ccttagatgc gtttcaattt gcccgttact
     7201 tcaacaaatc aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg
     7261 ttaacttttg tgtcagcgtg tccatccgct ctccttatta aggagcacaa caccagcacc
     7321 ttacccttac actgcctgtc gatactggtt tacgtttgtt gagtcgccat gttgcactcc
     7381 ggatcagagc actacctgcg gcaccacccg tccggtagcc aggacttgaa ataggttcga
     7441 tccggcgcaa cttcccaaca gtcaggatta taaatctcat tatcttaatt gcaacactgt
     7501 ccagaggtgg ttataattcg ttcgttggaa cacgatgttg cacaggcagt ggtgttaggc
     7561 ctgaaaatag taaataaaag aacctgcctc accagcagtt ttttttattt actgtgatct
     7621 gctttccaga tatttttcgc tcaaacaact aatgcgccaa acatttattg cgcgtaaaat
     7681 atcgtttatt tcattaatac atttcaggga tgaatatatg tcaccgcaga ataatcatct
     7741 gcagcgtccg cctgctgctg tgttatacgc cgatgaactg gcaaaattaa aacaaaatga
     7801 taacgcacct tgcccgcccg gttggcagtt aagtttgcct gcggcccgtg cttttatcct
     7861 tggcgatagc gcgcaaaata ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg
     7921 tatgttagtc actcttgcca ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc
     7981 aaaatctctc ctgtctgaat tactggcaac ctctattagc ggcgatgccg ggttaaccat
     8041 ccagggcggc gcatctacta ccgaagatca aatcaagtac ggctggaact acgccctgct
     8101 tatcaatcat ggtccatcaa cggaagcgct ggtccctgcc ccgctttacc agggaatgcg
     8161 cgacggcaaa atcgtccgtt ttgaagagat aacgcgtaca ccgctggaag tgcaagactg
     8221 tctgttaggt atgctttctg acagggtgat gacggtgccg gaactcactg gtgaagccag
     8281 tcagctctat gcgcgagaag gctttaatat tattgccact gccaataccc gcgatcgcgg
     8341 cgtcaatgaa atgagtgctg cgctaaagcg tcgcttcgat tttgaaactg tctttccgat
     8401 tatggatttc gcccaggagc tggagcttgt cgccagtgct tcggcgcgtt tgctggccca
     8461 tagcggtatt ccgcataaag tgcctgacgc ggtactggag ttactggtcc gcaccttccg
     8521 cgatctgcgt gccaacggcg aaaagaaaac gtcaatggat acactaacag cgattatgtc
     8581 caccgctgaa gccgtgaacg tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa
     8641 tcgcgcaggt gagccagccg acctggtgga ctgcattgcc ggaaccatcg tcaaagataa
     8701 cgaagaagat cgcgctcgtc tgcgccgtta cttcgaacag cgtgtcgcca cacataaaga
     8761 agctcactgg caggcttatt atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga
     8821 gcgagccgtt aattgtcggc atccggcatc atagtccggc ctgcgcccgg ctggtgaaat
     8881 cgttaatcga aagccagcgg ccacgatacg tgttgattga aggccctgct gattttaatg
     8941 accgggtaga cgaactgttt ttagcccacc agcttccggt agctatttac agttattgcc
     9001 agtatcagga cggtgcagcc cccgggcgtg gtgcctggac gccatttgct gaattttcgc
     9061 cggagtggca ggcgctacaa gccgatcgtc gtattcaggc acaaacttac ttcatcgatt
     9121 tgccttgctg ggcgcagagt gaagaagagg acgattcgcc tgatacgcaa gaggaaagcc
     9181 agaccttgct gctgcgtgcc acccgcatgg ataacagcga caccctgtgg gatcacttgt
     9241 tcgaagatga aagccagcaa actgcattac cctctgcact ggcgcactat tttgcccaac
     9301 tgcggggcga ctcccccggc gatgctctaa atcgtcagcg cgaagccttt atggcccgct
     9361 ggattggatg ggcgatgcag caaaataatg gcgacgtgtt agttgtctgc ggtggctggc
     9421 acgctccggc actggcaaaa atgtggcgcg aatgtccgca ggtaattaac acgccagaat
     9481 tgccgtcgct ggcagatgcc gttacaggtt gttatctcac cccctacagt gaaaagcgcc
     9541 ttgatgtgct ggcaggatac ctttcaggaa tgcctgcccc ggtctggcaa aactggtgct
     9601 ggcagtgggg cttacagcag gcgggagaac aactgctaaa aacggttctc acccgtttgc
     9661 gccagcacaa gttgcctgct tcgaccgcgg atatggctgc cgctcatctg catgcgatgg
     9721 cactggcaca gttgcgcggt catacactac cgttacgcac tgactggctg gatgccatag
     9781 caggatcgct gattaaagaa gccctgaatg cgccgttgcc gtggagctat cgcggcgtta
     9841 ttcatcccga taccgatccg attctgctaa cgttgataga cacattagcg ggtgacggat
     9901 tcggtaaact tgccccttct acgccacaac cgcctctgcc aaaagatgtc acttgcgaac
     9961 tggaacgtac cgcaatctct cttccggcgg agcttacctt aaatcgcttt aaccccaatg
    10021 gactagcgca aagtcaggtg ttacatcggc tggcaatact ggagatccca gggattgtac
    10081 gccagcaggg aagcacactg acacttgcag gcaacggtga agaacgctgg aaattaaccc
    10141 gcccgcttag ccagcatgcg gcattgattg aggccgcctg ctttggtgcc acactcctgg
    10201 aagccgcacg ccataaatta gaagccgata tgctggacgc gggtggaatc ggcagcatta
    10261 ccacatgtct tagccaagcg gcgtcagcgg gtctggcgtc cttcagtcaa caattactgg
    10321 agcaactcac attattaatc gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg
    10381 aagtgctata tgccttatgg cggctggatg aaattagcgg tatgcaaggc gcgcagatat
    10441 tacaaacgac gttatgcgcg gctatcgatc gcacgctgtg gctgtgtgaa tccaacggca
    10501 gaccggatga aaaggagttt cacgctcact tgcatagctg gcaagcgttg tgtcatatcc
    10561 tgcgcgatct acatagcggt gtgcaactcc ccggcgtttc gctttctgcg gcggtagcct
    10621 tgctggagcg acacagtcag gcgattcatg tcctggcact ggatcgcggc gcgactcttg
    10681 gtgcgctgat gcgtctggaa catcccaacg ctagtgccga agcggcgctg acgatgctgg
    10741 cgcagttatc cccggcacaa tccggcgagg cgctgcacgg tttgctggca ttagcccgtc
    10801 atcaactggc ctgtcagccg gcatttatcg ccggtttcag cagtcattta aatcaactaa
    10861 gtgatgccga ttttaccaat gccctgcccg atttacgcgc ggcaatggcc tggctaccgc
    10921 cacgagaacg cgggacgctg gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc
    10981 ccgtttcggc actgcaaatg ccgttgcatt gtccaccgca agccattgca catcatcaac
    11041 aactcgaaca gcaggcactg gcatcgctgc aacactgggg agttttccat gtctgaactg
    11101 aacgatcttc tgaccacccg tgagctacaa cgctggcgat taattcttgg cgaagcggca
    11161 gaaacgacgc tttgtgggct ggatgacaac gcccggcaga tagatcacgc gctggagtgg
    11221 ctgtatgggc gcgatcctga acggctccag cgtggtgaac gctctagtgg attaggtggc
    11281 tcaaatctca ccactcctga gtggatcaac agtattcaca cgctgtttcc gcaacaggtg
    11341 attgagcggc tggaaagcga tgccgtactg cgctacggca ttgaagatgt ggtgacaaat
    11401 ctcgacgtgc tggaacgtat gcagccttcc gaaagcctgc tacgcgccgt tttgcacacc
    11461 aaacatctga tgaaccccga agtactggct gccgcccgca ggattgtgcg ccaggttgtg
    11521 gaagaaatta tggcgcgact ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat
    11581 cgtcgtcgcc gctcatttat tccactggcg cgaaactttg atttcaaaag tactctgcgc
    11641 gccaacctgc aacactggca cccgcaacac ggcaagttgt atatcgaatt cccccgcttt
    11701 aacagccgca ttaaacgcca aagcgagcaa tggcaactgg tcttactggt tgatcaaagc
    11761 ggatcgatgg tcgattcggt gatccactct gctgtgatgg cggcctgttt atggcagtta
    11821 cccggcattc gtacccatct ggtggcgttt gacacaagcg tcgttgatct cacggcagac
    11881 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11941 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    12001 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    12061 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    12121 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    12181 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    12241 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    12301 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    12361 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    12421 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    12481 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    12541 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12601 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12661 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12721 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12781 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12841 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12901 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12961 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    13021 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagccac tatcatgccg
    13081 gagaatgctt acgtcagctt gcggcattaa acagtcgatt aaattgcgca caagagatgg
    13141 cccggagcga cagtgttggt gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg
    13201 gcattgccgg agaagcaaag cttgatcatc tgcggctggt gtctttaggt atgcgttgct
    13261 ggcaggatat tgagcattat ggtttacgca tctggtttac cgatcccgac accggcagta
    13321 ttttgcacct ttcgcgcagt tggccgcgaa gtgaacagga agactcaccg gcagctacgc
    13381 gtcggctgtt tagttttcag gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag
    13441 caaaacgcag tgccgatggc gagctgctgt tagctacccg caaccgctta agcagcgttg
    13501 taccgctgtc gcctgatgcc tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg
    13561 tggctttgcg ggaatattta cgccagcgtc cccccgcctg catacggcct cttaatcagg
    13621 tcgataactt atttattctg ccggtcgctg agtgtatttc gctcggttgg gatagcagcc
    13681 gccagacgct ggatgcgcag gtaatcagcg gcgaaggtga agataatctg ctgacgttat
    13741 cactgccagc ctcagccagc gcgccttatg ccattgaacg catggcggcg cttttgcaac
    13801 aaacagacga ccccgtgtgt ctggtttctg gctttgtcag ttttgttgat gggcaattga
    13861 cactggaacc acaggtaatg atgacaaaga ctcgtgcctg ggcgctggac gcagaaactg
    13921 cgcctgtggt ggtatcacta ccttctgcca gcgttctgcc tgtgccgtct actgctcatc
    13981 agttgctgat gcgctgccag gcgttactta ttcaactgct ccataacggc tggcgctatc
    14041 aggaacagag cgctattaat caggcagagt tgctggcgaa tgacctcacc gcggtgggtt
    14101 tttatcggct ggcacatgtg ttggcacaat ttcgtaatac agaaagcgag gcacgggtag
    14161 aagcaatgaa taacggtgtt ttgctttgcg aacaattatt ccccatgctt cagcaacaag
    14221 gatgaaatag tgctttttac taagagttct actccagttc cggactgctc acgccacggt
    14281 attaggcata tcctatatag cccctggtga gagtcacc
//
LOCUS       DAZPEU010000096        14110 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPEU010000096 DAZPEU010000000
VERSION     DAZPEU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.097"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..415
                     /locus_tag="ACHU2L_002546"
                     /pseudo
     CDS             1..415
                     /locus_tag="ACHU2L_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555244.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            580..4698
                     /gene="pic"
                     /locus_tag="ACHU2L_002547"
     CDS             580..4698
                     /gene="pic"
                     /locus_tag="ACHU2L_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR1357367.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4849..5091)
                     /locus_tag="ACHU2L_002548"
                     /pseudo
     CDS             complement(4849..5091)
                     /locus_tag="ACHU2L_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5130..6080)
                     /locus_tag="ACHU2L_002549"
                     /pseudo
     CDS             complement(5130..6080)
                     /locus_tag="ACHU2L_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6131..6706)
                     /locus_tag="ACHU2L_002550"
                     /pseudo
     CDS             complement(6131..6706)
                     /locus_tag="ACHU2L_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6710..7110
                     /locus_tag="ACHU2L_002551"
                     /pseudo
     CDS             6710..7110
                     /locus_tag="ACHU2L_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7513..8094)
                     /locus_tag="ACHU2L_002552"
     CDS             complement(7513..8094)
                     /locus_tag="ACHU2L_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357368.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHU2L_002553"
     CDS             complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHU2L_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR1357369.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12359..13363)
                     /locus_tag="ACHU2L_002554"
                     /pseudo
     CDS             complement(12359..13363)
                     /locus_tag="ACHU2L_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13348..13725
                     /locus_tag="ACHU2L_002555"
                     /pseudo
     CDS             13348..13725
                     /locus_tag="ACHU2L_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13764..>14110)
                     /locus_tag="ACHU2L_002556"
     CDS             complement(13764..>14110)
                     /locus_tag="ACHU2L_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1357370.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3541 a         3317 c         3459 g         3793 t
ORIGIN      
        1 catcgtgtgg tgacgagtct catcgtctat ggaaacgaca ttcgtgctgg atgcactgga
       61 gcaggcgttg tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca
      121 gtatgtgtca ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg
      181 gagtactggt gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc
      241 ggaggtaata caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac
      301 gtgggtggac tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc
      361 agaagcagaa aaagcttatt atgcttccat cggaaatgat gatctggcag cctgagttca
      421 cagataaaac actctccagg aaacccgggg cggttcagtt cacaaaaaca cattaatgca
      481 gtaactatat tttcctttct ggtgataacg tcgggttatc attagcttct tcagctattt
      541 tacttttata tcccttgtaa acatcatgga gaatccatag tgaataaagt ttattctctt
      601 aaatattgcc ccgtcaccgg ggggcttatt gctgtctctg aacttgcccg cagggtaata
      661 aaaaagacat gccgaagatt aacgcatatt cttctggctg gcattccagc aatctgtctg
      721 tgttactctc agatatccca ggcgggtatt gtccgttccg atattgccta tcagatttat
      781 cgtgatttcg ccgaaaacaa agggcttttt gtacctggtg ccaatgatat tccggtatat
      841 gataaggacg gaaaacttgt gggaagactg ggtaaagccc caatggccga tttcagcagt
      901 gtgagctcaa atggcgttgc tacgcttgta tcgcctcagt atatcgtcag cgtaaagcat
      961 aacggaggat atcggagtgt gagctttggt aatgggaaaa atacatattc ccttgttgac
     1021 cgtaataacc acccttctat tgacttccat gctccacgtc tgaataaact ggttacagaa
     1081 gttattccct cagcggtaac atcagaagga accaaagcca atgcttataa atacactgaa
     1141 cgttacaccg ctttttatcg ggtgggtagt ggtacgcagt acactaagga caaggacgga
     1201 aatttagtta aggttgccgg tggatatgct tttaaaacag gaggaaccac aggagttcct
     1261 ctgatatctg atgcaacaat agtctctaat cccgggcaaa cttataatcc tgtaaacggc
     1321 cctttacctg actatggagc ccctggggat agtggttctc ctttgtttgc ttatgataaa
     1381 caacaaaaaa aatgggttat tgttgctgta ttaagagcat atgcaggtat taatggtgct
     1441 acgaactggt ggaatgtcat accaacagat tatctgaacc aggttatgca ggacgatttc
     1501 gatgcccccg tagactttgt ttccggactg ggccccctga actggacata cgacaaaaca
     1561 tcaggcacag gtaccctgag ccagggcagt aaaaactgga ccatgcacgg gcagaaagac
     1621 aatgacctca atgccggtaa aaatctggta ttcagcgggc agaatggtgc aattatcctg
     1681 aaagacagtg tgactcaggg tgccggttat ctcgaattta aagacagtta caccgtatct
     1741 gctgaatccg gaaaaacatg gacgggtgcc ggcattatta ctgacaaggg gacgaatgta
     1801 acctggaagg tcaacggcgt tgccggtgac aacttgcata agctggggga aggaaccctg
     1861 accataaacg gaacaggtgt aaacccggga ggactgaaaa cgggagacgg tatcgttgta
     1921 cttaaccagc aggcagacac tgcaggtaat atccaggcct tcagttcagt gaacctcgcc
     1981 agcggacgtc cgaccgtggt gctcggggat gcccgtcagg tcaatccgga taacatttca
     2041 tggggatacc ggggaggtaa gcttgacctt aatggtaatg ccgttacctt cacccgactg
     2101 caggctgctg attacggggc ggtgattaca aataatgcac agcaaaaatc ccagctttta
     2161 ctggatctta aggctcagga tacaaatgtc agtgaaccga cgattggaaa tatatccccc
     2221 tttggtggta ccggcactcc aggaaacctg tacagcatga tactcaacag ccagacccgc
     2281 ttctatattc tgaaatctgc cagctatggt aacactctgt gggggaacag cctgaatgat
     2341 ccggctcagt gggagtttgt tggcatgaac aaaaacaaag cagttcagac agtaaaagat
     2401 aggatcctgg ccgggcgggc aaaacaaccc gttatctttc atggtcagct gaccgggaat
     2461 atggatgtcg ccattccaca ggtgccgggg ggaagaaagg taatctttga tggtagcgtg
     2521 aacctgccgg aaggtaccct gagtcaggac agtggcaccc tgatattcca gggacatccg
     2581 gttatccatg cctccatcag tggcagtgca ccggtcagcc tgaaccagaa agactgggaa
     2641 aaccgtcagt ttacaatgaa aacactgtcg ctgaaagacg ctgacttcca tctttcacgt
     2701 aacgcctcgc tgaacagtga cattaagtcg gataacagcc atatcacact gggaagtgac
     2761 agggcatttg tggataaaaa tgacggaaca ggaaattatg tcattccgga ggaaggtacc
     2821 tctgtcccgg acaccgtgaa tgacaggagc cagtatgaag ggaatattac gctgaaccat
     2881 aactcagccc tggatatcgg cagcaggttc accgggggga ttgacgctta tgacagtgcc
     2941 gtcagcatca cctctccgga cgtcctgttg acagccccgg gtgcttttgc cggcagttca
     3001 ctgacagtgc atgatggcgg tcatcttaca gcactgaacg gtcttttcag cgacgggcat
     3061 attcaggccg gtaagaacgg caaaatcacc ctgagcggta caccggttaa agatacggct
     3121 aatcagtatg cccctgctgt atatctgacg gacggatatg acctgaccgg cgataacgca
     3181 gcactggaaa ttacccgtgg agcacatgct tccggtgata ttcatgcctc tgcggcatca
     3241 acagttacca tcgggtctga cacgccggca gaactggctt ctgcggaaac ggctgcatcg
     3301 gcgtttgccg gcagtcttct tgagggctat aacgcagcat tcaatggtgc cataaccggt
     3361 ggcagggctg atgtcagtat gcataatgca ctgtggactc tgggtgggga ctctgccatc
     3421 cacagtctta ccgtcagaaa cagccgtatt agttctgaag gagaccgtac attccgtacc
     3481 ctgacggtga ataaactgga tgcaacaggc agtgattttg ttttgcgtac ggacctgaaa
     3541 aatgccgata aaattaatgt gactgaaaaa gccactggtt cagataacag cctgaacgtc
     3601 agctttatga ataatcctgc tcagggacag gccctgaata ttcctctggt cacggcaccg
     3661 gcgggaactt cagcagagat gtttaaggcc ggcacccggg tgacaggttt cagtcgggtg
     3721 accccaaccc tgcatgttga taccagtggt ggcaatacga agtggatact ggatggtttt
     3781 aaagcggagg ctgataaagc cgctgccgcg aaggctgaca gtttcatgaa tgccgggtat
     3841 aaaaacttca tgacggaagt taacaatctg aacaaacgta tgggtgacct gcgtgacaca
     3901 aacggtgatg ccggtgcctg ggcgcgcatc atgagtggtg ccggttctgc agacggtggt
     3961 tacagtgata attacaccca tgttcaggtc ggctttgaca aaaaacatga actggacggt
     4021 gtggacctgt ttaccggtgt cacgatgacc tataccgaca gcagtgcaga cagccatgca
     4081 ttcagcggaa agacgaaatc ggtggggggc ggtctgtatg cttcagcatt gtttgagtcc
     4141 ggtgcctata tcgatttgat tggtaaatat attcaccatg acaatgatta cacaggtaac
     4201 tttgctagcc tgggaacgaa acactacaac acccattcct ggtatgccgg tgctgaaacg
     4261 ggttaccgct atcacctgac agaggacacg ttcattgagc cgcaggctga actggtttac
     4321 ggcgccgtgt ccgggaaaac attccgctgg aaagacggtg atatggacct gagcatgaag
     4381 aacagggact tcagtccgct ggttggaaga acaggggttg aactgggcaa gaccttcagt
     4441 ggtaaggact ggagtgtgac ggcccgtgcc ggaaccagct ggcagtttga cctgctgaat
     4501 aatggagaga ccgtactgcg tgatgcgtcc ggggagaaac ggataaaagg agagaaggac
     4561 agccggatgc tgtttaatgt tggtatgaat gcgcagataa aggacaatat gcgctttggt
     4621 ctggagtttg agaagtcagc ctttggtaaa tataacgtgg ataatgcggt aaacgcgaat
     4681 ttccggtata tgttctgatg tgggggtaaa gggctgccgg aaggcagcct ttctcagaga
     4741 ctcttaaaat ataaaactga aggtcttcgg taagggggag tgtattagac gtcaactagt
     4801 tttgccgact ggcgcgagcc tgtttaagat tctgtgtaaa tgcctttctc agaagtgacc
     4861 gtccaggcgg tcaccgaact cgataataaa gcggctcatt gccattcgcc agtccttcaa
     4921 cggcatcgtc catttctggg acgcagactg gattgccagc cacaccactt ttttcaccga
     4981 gtcgtctgtc gggaacactt tacgcttttt gatcgcatgg cggatcacgc tgtttagcga
     5041 ctcgatggca ttcgtcgtat agatcacttt gcggatgtcc gttggataag cggtaagcgt
     5101 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     5161 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     5221 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgcg
     5281 ccccgctcac caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc
     5341 gcccgcaggg cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac
     5401 acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga
     5461 cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg
     5521 gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5581 atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5641 gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg
     5701 ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa
     5761 ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc
     5821 gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5881 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5941 ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     6001 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     6061 atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg
     6121 gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc
     6181 gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac
     6241 cattactcca gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg
     6301 ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta
     6361 gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta
     6421 ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     6481 catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc
     6541 tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga
     6601 cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt
     6661 tataattacc actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac
     6721 gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga
     6781 cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac
     6841 tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg
     6901 gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggga
     6961 gcgctactgt acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac
     7021 cttcgccatg tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt
     7081 ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaatacggc cctcgctgta
     7141 cgcttactca tcacctgcta ttaatccccg agtcagtact tcgcggcctg acgcgatcac
     7201 cctactcagt acttcagagc ccgcctccgg tacctgcctg gcgatcgccc ctgtgcaaca
     7261 aaataaatca accgttatca aaccacgatc gttcgtatgc gccgccgatg acgcgcgcct
     7321 gaaaaacgcg tcaatatgag ttccccggaa gcttacgata atacgcttat gaacattaca
     7381 acacgctcat gcggaacctg gatgggggaa ggaacggtga agctgttagc gaatgaccgg
     7441 cgtttataca aacgccggtc atgatgaatg atatttcaga atatacctcg ggctaaaata
     7501 ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa gtactgagct
     7561 tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc cctgaaataa
     7621 tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca tatttttaat
     7681 gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac tgagtctgat
     7741 gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt cagtgaccag
     7801 catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg tgattgtaat
     7861 gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc cgtatcggaa
     7921 ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt tttgcctgat
     7981 agtttctttt tttattcgct catgtttaag gcataacagg cagataaata atgtcagaaa
     8041 aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat acatattctc
     8101 tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag taacggaagt
     8161 tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca aactcaagac
     8221 cgaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga atacgaccgt
     8281 ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt tcaccgttgg
     8341 caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag tccttaccgg
     8401 agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag tccttatcct
     8461 tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat acggccccat
     8521 aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag cggtagcctg
     8581 cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagt ccagcaaagg
     8641 tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata taggcaccag
     8701 agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg ccactgaatg
     8761 cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa aggtcaagtc
     8821 catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg tcactgtatc
     8881 caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccggcc tcgccattgg
     8941 tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg aaggctttgt
     9001 aattaccgga catatacgat tttgctgcac cggaagatgc tgtattttca gccaccttat
     9061 atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc acaggagata
     9121 tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg cttccttttg
     9181 gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc tgtatatcga
     9241 ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta tccgattgat
     9301 ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca tccagtgtgt
     9361 ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa agactgtcgc
     9421 gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg ttatacatgc
     9481 tggctgaggc tgcaggagca tcaatacctc cggtataaga agtatcaaag ccacccagca
     9541 aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta tactggtcaa
     9601 caccaaacag aatagatgct tttttgtctg attttatatt accattgacc caggcataat
     9661 tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac atatagattg
     9721 ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt ccggccagtt
     9781 caatagcttt atctacatac atttttccct gggcggtaac agtagctcct ttgttgataa
     9841 tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat ttatcaccaa
     9901 cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg cctgctgaat
     9961 ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt cttcctgaga
    10021 acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta gcctctatgt
    10081 gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa tttaatttcc
    10141 ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc tgattgttgg
    10201 acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt atggcagcag
    10261 aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct ttaaatatgg
    10321 catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag atatcgtttt
    10381 tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc tgggtagtat
    10441 tgataatatg atttatgctt atattacctg atacattccc atggtacata tatgcatcag
    10501 tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt gttcccgcat
    10561 ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt gttccgccat
    10621 ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg gcatttattt
    10681 ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca gcactaagga
    10741 tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca tccagagtac
    10801 cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat tcaacggtgg
    10861 tattattatc aatatctatc ccggcacctt tataagactt atcttttccg tacactttat
    10921 atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc tgcttgagcg
    10981 ttaatacccc tcctcctgag acagacaggt ccttatcttt tataatcgat gataactctt
    11041 gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg tttaccagaa
    11101 tattattaaa taacgagtag tttgtccagg ctatattggt actactgata aaaccaactg
    11161 tactggtaac accatggaaa acccattttt gttgttcttt atcatataga taagaaccag
    11221 aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta taaatgtaac
    11281 tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga ttatagtttt
    11341 gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca cctgctctga
    11401 agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc gcatcttcgg
    11461 gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg aatttattca
    11521 gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat gctgtctgat
    11581 cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca gtaacactgt
    11641 acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa tcaggtacag
    11701 gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca tttgttgcac
    11761 ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc catatattat
    11821 ttatatccag cattgctgca aatgctgttt cgccataact tccatatatt gaggataaaa
    11881 taataccgag gataactttc tttcttgatg taccgacact aactttccgg gtcagttcag
    11941 aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt ttattcatac
    12001 gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca acggggaact
    12061 atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt tatatttgta
    12121 tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg ttagatattg
    12181 atcacttggt gtttgtattt tgaattttgc gatcaaaatt tgatctttgt tgtttccggt
    12241 caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat gtccgacaga
    12301 cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca ggtggaactc
    12361 agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg atcaggcctg
    12421 caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg aatgcactgc
    12481 cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat ttccaggcat
    12541 taaattctgt gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa ccgctggccg
    12601 gggtcagatc cacatcaaat gcagtggtga acatcgcgtt gtgccaggag acaccggccg
    12661 tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt gtctcaatac
    12721 tacgggagat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc aggtccgtac
    12781 tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg cctttgaagg
    12841 cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg cgctggaatt
    12901 tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta gccagagcaa
    12961 tacccacatc agtgatcagt gccgtgcggg cacaggcctg tgatgtcagg cggcttttgt
    13021 ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca gcatcagaga
    13081 ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca gagaggcgat
    13141 caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt gtgttacgga
    13201 agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg ttactttcga
    13261 tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg gtgatattat
    13321 ccggattgga gagctgcgcg ccgacggacg gaagcaggat gctgggctca tgcgcgacgc
    13381 aaaatccacg atgtatatat cagtactaaa agcgcgacgg cggaagaagc tctgaaacta
    13441 atcggtgaac tgtacgccat agagcacgat atacgcgggt taccggtgtc tgaacgcctg
    13501 gcggtcaggc aaatgcagag tagaccgctc ctgacttccc tgtataagct gatgcaggag
    13561 aaagaacaca cgttatcgaa gaaatgtcgt ctgggagatg cgttccggta tatcaggaag
    13621 cactgggctg cgttgtgcaa cttctgtgat gatggcctgg ctgaggtaga taataatgca
    13681 gcagaaagag cgcttcgtgc agtctgtctc gggaagaaaa ctttttcgag gatcactcat
    13741 tccgtgtctg actgcttcag ggtttaattt tgcttctgct gactgaatga tgattgggca
    13801 gcaagtgcaa tatcccacat aaatccagtc aactcacgag ccaccgccgt aatggctact
    13861 tgtgattttt tcccttttgc tgtcagagtt cggtaacgag aacacaatcg ttgttgagct
    13921 ttccatgcaa tatctttgga atattgtgtt acagagtccg gtcggtaata tataagccat
    13981 gctccaactt ttgcaggtgt acgataagac caggcggctt cgtaaagcag acgtctcagt
    14041 tcgctgtttc caacttttgt tattcctctg ggacgaatac tgtttccact ggaatattca
    14101 cctggcacca
//
LOCUS       DAZPEU010000097        13975 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPEU010000097 DAZPEU010000000
VERSION     DAZPEU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.098"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHU2L_002557"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHU2L_002557"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR1357371.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHU2L_002558"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHU2L_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR1357372.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHU2L_002559"
     CDS             2630..3391
                     /locus_tag="ACHU2L_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR1357373.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHU2L_002560"
     CDS             complement(3442..4326)
                     /locus_tag="ACHU2L_002560"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR1357374.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHU2L_002561"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHU2L_002561"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1357375.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHU2L_002562"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHU2L_002562"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR1357376.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHU2L_002563"
     CDS             6202..6474
                     /locus_tag="ACHU2L_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR1357377.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHU2L_002564"
     CDS             6844..7920
                     /locus_tag="ACHU2L_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1357378.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHU2L_002565"
     CDS             7947..9326
                     /locus_tag="ACHU2L_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1357379.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHU2L_002566"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHU2L_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACHU2L_002567"
     CDS             10409..11245
                     /locus_tag="ACHU2L_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR1357380.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACHU2L_002568"
     CDS             11250..12197
                     /locus_tag="ACHU2L_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1357381.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHU2L_002569"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHU2L_002569"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR1357382.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..>13975
                     /locus_tag="ACHU2L_002570"
     CDS             13324..>13975
                     /locus_tag="ACHU2L_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1357383.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAF"
BASE COUNT         3664 a         3101 c         3568 g         3642 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 taatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttg
//
LOCUS       DAZPEU010000098        13871 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPEU010000098 DAZPEU010000000
VERSION     DAZPEU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.099"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..455
                     /locus_tag="ACHU2L_002571"
                     /pseudo
     CDS             1..455
                     /locus_tag="ACHU2L_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            515..622
                     /locus_tag="ACHU2L_002572"
     CDS             515..622
                     /locus_tag="ACHU2L_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR1357384.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(603..1334)
                     /gene="yehW"
                     /locus_tag="ACHU2L_002573"
     CDS             complement(603..1334)
                     /gene="yehW"
                     /locus_tag="ACHU2L_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR1357385.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1339..2265)
                     /gene="yehX"
                     /locus_tag="ACHU2L_002574"
     CDS             complement(1339..2265)
                     /gene="yehX"
                     /locus_tag="ACHU2L_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR1357386.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(2258..3415)
                     /gene="yehY"
                     /locus_tag="ACHU2L_002575"
     CDS             complement(2258..3415)
                     /gene="yehY"
                     /locus_tag="ACHU2L_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR1357387.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="ACHU2L_002576"
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="ACHU2L_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR1357388.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="ACHU2L_002577"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="ACHU2L_002577"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR1357389.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="ACHU2L_002578"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="ACHU2L_002578"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1357390.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="ACHU2L_002579"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="ACHU2L_002579"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR1357391.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="ACHU2L_002580"
     CDS             complement(9943..10530)
                     /locus_tag="ACHU2L_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR1357392.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="ACHU2L_002581"
     CDS             10700..11278
                     /locus_tag="ACHU2L_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1357393.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12141)
                     /locus_tag="ACHU2L_002582"
                     /pseudo
     CDS             complement(11395..12141)
                     /locus_tag="ACHU2L_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="ACHU2L_002583"
                     /pseudo
     CDS             complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="ACHU2L_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
BASE COUNT         3299 a         3888 c         3500 g         3184 t
ORIGIN      
        1 cggcaatcta catagcctgc gaacgtggat caaagagcgc ggtcaggatt accccgccca
       61 gacactcacc acacatctgt ttattcctct gcgccgacgg cttcagtgcc aacaaccgac
      121 tctccaggcg ctgctggcga tcctcgacgg cgtactgatc aactacatcg ccatttgtct
      181 ggcttcggca cgtaaaaaac agggtaaaga tgcgctggtg gttggctgga atattcagga
      241 taccacccgt ctgtggctgg agggctggat tgccagtcaa caaggatggc gcattgatgt
      301 cctcgctcgc ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc
      361 tggtgtggtg cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag
      421 aacaaggcca tgatattttc ccactcggta tttaatgatt cgttaacaaa tgcgctttac
      481 tgtacaatcc tttcgttaac ataaggagtg cattatgcgc atagctaaaa ttggggtcat
      541 cgccctgttc ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac
      601 ctttattttg cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg
      661 atcgccgcca gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta
      721 agcccgatga tgatgggcgt acccagcgta ctggccccta ccgttgaggc gatcgtcgcc
      781 gtaccaatgt tgataatcac cgaagttcgc acgcccgcca gaatcaccgg agccgccagc
      841 ggtagctcga ccttacgcag tcgctgacct cgactcattc ccataccttt cgcaacttct
      901 gtcacgctgg gatcaatcgc tcccagcccg gcaagtgtca cctgcaggat gggcagcaca
      961 ccgtaaagga tcaaggcgat aatcgctggt tgcagaccaa agccgatcac cggaacggca
     1021 atcgccagca ctgcgacagg cggaaaagtc tgtccaacgg cggcaatagt ttccaccagt
     1081 gggcgaaatt ccgcgcccca cgggcgagta acagcaattc cggcacctgt gccaatgatc
     1141 accgcaaaca aactcgaaat tcccaccagc cagaaatgag ccagtgccag agctgcaaaa
     1201 ctttcttgct gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg
     1261 ctgtaaggca gccagaaaat cagcgccaca aaaagagcaa tgagccagaa cagcggatcg
     1321 cgcaacatct tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtgccgca
     1381 aggctggccc tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga caaacaggga
     1441 gagcgcatcg cgtagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg
     1501 acgcacgtaa tccgccacgc tacgtaacga aagcaggtgc acccccagtt cactacgtcc
     1561 aaaaaactgg cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg
     1621 ctgcaccact tcaccgtgat ccatcaatac cagatgttct gccagccgta gcgcctcatc
     1681 aatatcatga gtgaccagca caatagtgcg ccccagcaaa cggtgaatgc gcgtcatctc
     1741 ttgttgcaac gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa
     1801 gacttgcgga tcggcagcca gtgcacgcgc cactcccaca cgttgctgct gaccaccgga
     1861 aagctgatgc ggataacgct cacgcaaatt tgactccagc cccagtagcg ccattaattc
     1921 gtcgatacga tcgtcaatcc gcgcccgcga ccatttttgt aattgcggca cggtagcgat
     1981 gttttgcgcc acgctccagt gggggaacag gccaatagat tgaatggcat agcccatccg
     2041 gcggcgcaac tccagtactg gcagcgagcg aatttcttct ccggcaaagc ggatcactcc
     2101 gctgtcatgc tccaccaggc ggttaatcat tttcagggtg gtggatttgc cggagccaga
     2161 tgtgccaatc agcaccgaaa aactcccttc ctgaaaattg agattgagat cgttaacggc
     2221 tttttgtgcg ccgaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca
     2281 gcagtgcgat aagcaaatcg aacagcgcgt cggtaagcac cgccagaaca attaccggga
     2341 tcacccccag caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac
     2401 caaaaccgcc tgcgccgatt aacgccgcaa tcaccgccat acctacagtt tgcaccatca
     2461 ccacccgcag gctgcgcaga aataccggta acgccagcgg taactgaaca tgcaggaaac
     2521 gctgcgcccc gctcatcccc atcgctctgg cgctctccag cacatcgcgc gggatctggt
     2581 tcaaaccgac taccacgcct cgcaccagcg gcagcaaggc atagagcacc agcgcaatca
     2641 gtgcgggtgt cattccggtt cctgctatgc cgagcttccc cagccacgga aaggccgtaa
     2701 ccagcgcggc aagcggcgca atcaacaggc caaagagcgc caccgaaggc acggtctgaa
     2761 tgacattgag cagagaaaaa attgccccct gccgcgcagt ggaaaagtag caccagatgc
     2821 ccaacggcac accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt
     2881 gtgccagcgc gtcgtcaaac acatcctgac ggttggcgta ttctttcatt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgcagc agccagccag aaacctctgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg accgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg tgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcatc ggaagtaatc gccccgcgcc
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgctcc
     4981 gttagcttca tcaaaataac gcgaagtata tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggaaat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 caccaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac cgtcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaatgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccaag ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca aactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc acagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccgcgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccacaccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcaataacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 aaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggcaaagggc ttcgtttttt cctatactta ttcagcactc acaaataaag
    13861 gaacgccaat g
//
LOCUS       DAZPEU010000099        13833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPEU010000099 DAZPEU010000000
VERSION     DAZPEU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.100"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="ACHU2L_002584"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="ACHU2L_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR1357394.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /locus_tag="ACHU2L_002585"
     CDS             322..1953
                     /locus_tag="ACHU2L_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR1357395.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="ACHU2L_002586"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="ACHU2L_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR1357396.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /gene="dcuB"
                     /locus_tag="ACHU2L_002587"
     CDS             3240..4580
                     /gene="dcuB"
                     /locus_tag="ACHU2L_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR1357397.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /gene="fumB"
                     /locus_tag="ACHU2L_002588"
     CDS             4658..6304
                     /gene="fumB"
                     /locus_tag="ACHU2L_002588"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1357398.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6427..7056
                     /locus_tag="ACHU2L_002589"
     CDS             6427..7056
                     /locus_tag="ACHU2L_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR1357399.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7195..8616)
                     /gene="melB"
                     /locus_tag="ACHU2L_002590"
     CDS             complement(7195..8616)
                     /gene="melB"
                     /locus_tag="ACHU2L_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR1357400.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8719..10074)
                     /gene="melA"
                     /locus_tag="ACHU2L_002591"
     CDS             complement(8719..10074)
                     /gene="melA"
                     /locus_tag="ACHU2L_002591"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR1357401.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10357..11265
                     /gene="melR"
                     /locus_tag="ACHU2L_002592"
     CDS             10357..11265
                     /gene="melR"
                     /locus_tag="ACHU2L_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR1357402.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11464..13731
                     /gene="adiA"
                     /locus_tag="ACHU2L_002593"
     CDS             11464..13731
                     /gene="adiA"
                     /locus_tag="ACHU2L_002593"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR1357403.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3611 a         3585 c         3357 g         3280 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaagct gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gagttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagcc aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtcacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctacgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg atcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaacaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaatcgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagcg cctggcagca tgctgccagg tgatccccct gggccacctc
     6361 ttttgcgatt gtaatttcat gcttgctggt gaatagtcag tattttccct gacttgcgaa
     6421 ctcaccatga cccgcacact caagccgtta attcttaaca ctggcgcact ggcgctaacg
     6481 ttaatcctga tttataccgg catttcggcc catgacaaac tcacctggct gctggaagtg
     6541 acaccggtga ttatcgtcgt gccgctacta cttgccaccg ccagacgtta cccgttaacg
     6601 ctgctgctct atacgctcat cttctttcac gccatcatcc tgatggtagg cgggcagtac
     6661 acctacgcga aagtccccgt tggttttgag gtgcaggaat ggctggggtt gagtcgtaat
     6721 ccgtatgaca agctggggca ctttttccag gggctggtgc ctgcactggt ggcacgagaa
     6781 attctcgttc gcgggatgta cgtgcgcgga cgtaaaatgg tggcgtttct ggtctgttgc
     6841 gtggcgctgg cgataagcgc gatgtatgaa ttaatcgagt ggtgggcggc actggcgatg
     6901 ggccagggag cggatgattt tctcggtacc cagggcgacc agtgggacac gcaatccgat
     6961 atgttctgcg cgctgcttgg cgcattaacg acagtgatat tgctcgctcg ctttcattgc
     7021 cgccagctac ggcgctatgg cttgataact ggataacaac gtgcactttg ttgatgccgg
     7081 atgcggcgtg aattccttat ccggtctaca aaatttgcaa atccaaagca ttacattaag
     7141 acgtggcctg ataaacatat cgcatcaggc cattttcgtt agctattgtc cacgtcaggc
     7201 tttcacatca ctcactgcac cgaccggaat atcagcatga acaggctcgg gcggtacttt
     7261 gcgatattta tccagcagat gaatctggat cctgcgcagc gtgtcgccat tgaggcgata
     7321 gaaacggaag tagagaatca gcgttaccat gaaaaacaga gtcggtagag caatcataat
     7381 aaactgcata cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat
     7441 ccctaacaca accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt
     7501 ctgcacggag taagcgatgc tttcacagcg tacgtgcagt ttatattcac cgtaatcaac
     7561 ggtatctgcc accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa
     7621 aatacccgca atcacaatga ggacgacgtt gtgatagctc attaatgcca ttaacaggag
     7681 aacaccacag cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa
     7741 tgatttaact aagcgtggga agaatactaa cgtcaccagg ttagcagctc ccgcatacga
     7801 cagataatag gggaacaaat ccgcatcacc gataacatat gagaaataat agatagcaaa
     7861 tccggtaata atgttgctgg ctacattata agcaagagcc atacccaaga ggcatgaaag
     7921 ctgatcgttt ttataaataa gcgcaacgat ggctttaagt gtcagatggc ttccttcagc
     7981 agacgattga ttgtctgacg aaaagacttc atgcacattg cgcagagtga tgatggttga
     8041 aacaataaaa aaggcgatca ataccagagt gaacatctga aagccaaatc cccgatcgcc
     8101 accgccgaca taattaacaa atggtagcgt cacacctgcc gtaacaaagc ctgccagact
     8161 ggcaaaaaaa cgcggataag gaaccagttg ttcgcgttca cgtttatcga gcgtgatggt
     8221 tggaaccagc gaccagaagg gaatatccat aatggtgtaa gtcatgcccc agaggatgta
     8281 ggtcacgcaa acaaagacaa tctgagtagt accttcaaac agatgcgcac taaagaggag
     8341 aaataagatt acagagtttg ccaacgtacc gatcaggatc cagggtttga acttacccca
     8401 tcgcgatcgc gtagcattta caatccatcc cataatcgga tcgttaatag catcccagat
     8461 cctcgccacc agaaacaaag taccgaccaa acccacagac agcccgacga catcggtgta
     8521 gtaatacatg aggtacatat acacaatgcc gatcgcaaaa tccttcccga acgctccaaa
     8581 tccataactg agttttgtag tcattgaaat gctcataggg tatcgggtcg ctgttaccaa
     8641 cgcccgcctt ctgttattgg tatcgtcaga ggtgtgcgcc ggaataaatc ccccggcgca
     8701 gtagcgttta gtcgcgtttt aacggtgcaa ccagcttggc agccagtcgc cgtgggcggc
     8761 aatcaggtcg tcaacaagag catatatttc gtcaatgccc agcacggcgg cagtatgcgg
     8821 gtccatcatc gcggcgtggt aaacacggtc gcgattttcc gtaagaatag cttcggtcag
     8881 cagcgtctgt acgttgatgt tggtttgcat cagggcggcc agatgcgaag gtagcgtacc
     8941 gactttggtc ggctgaatgc cattagcatc aaccagacag gctacttcca cgcaacatcc
     9001 ttgtggcagg ttatcaatca aaccatcgtt acggacgttg ccgtaaatca cactcggctc
     9061 gccagtccag atagcgttca tgattgtgct ggcatattcc cgtgacggtt taatatcaat
     9121 ccgggaggcg tttttatact cctccagctc tttatgccag ttcgccagct gctcgacgca
     9181 gcgtttcggg tactcatcca gcggtacttt ataacgctca atcaaatcct cacgacctag
     9241 cttaataaac cacggcgtat attcggcaaa atgttctgat gattccgtga cgaagtagcc
     9301 cagctttttg aacatttcgt agcgcacaat attctggcag cgagtattac catgaatatt
     9361 tggcttcggt gcctgccctg cttcataagc cgccagcagt tccgggtaga gattcacgta
     9421 actgccgtcg gcggttttgc gctccagctc caggtaaaac gccatatggt tgatacctgc
     9481 gcaacggtaa cgcagcgtag ccgggtcgat attgaggtca cgcgccagct cttccgccgt
     9541 tccctgcacc gaatggcaca gcccgacctg tttgatatac ggatagcggg catacatcgc
     9601 ccaggtattc atcgccattg ggttaacata gttgagcatg gtggcatcgg ggcagacttc
     9661 cgtcatgttc tcgcaaattt gccacagatg cggaatggta cgtagcgcgc gcataatacc
     9721 gcccggcccc aacgtatcgg caatggtttg ttccagacca tgccgcttac agacctcgaa
     9781 atcagtcacc gtgcaaggtt cataaccgcc aatctgaaat gccaccacga caaaatcggc
     9841 atcctctaag gcttctttct gttgggtgtg gcaggtgatt ttgccgctgg cccctgctga
     9901 atccatcagc ttacgcacca caatatgcga ctcttccagg cgggtggggt caatgtccat
     9961 cagggcaata tgcgccgttt tcagcgcctc gcgatggaac acatcaccaa gaatattttt
    10021 aacgaaaatc gtcgaaccag cgccgataaa tgtaattttg ggtgcagaca tcatgcagat
    10081 ctcctggctt gcttgaataa cttcatcatg gcaggcgaat ctgcagaaaa cctccggctt
    10141 ccaggttgag cattcccata aactcagatc ctgcgtgaag cagcagtaaa tctgagttta
    10201 tgggaataaa ttacctgaaa agtagagttt tcggcctcat gatgctcgga cagtcccctc
    10261 gcccctctgg gaagagggtg aaaaccgtgc tcccactcgc agtcatcctc cctcactcct
    10321 gccataattc tgatattcta ggaaagagag ccatccatga atacagatac gtttatgtgc
    10381 agcagcgacg aaaaacagac ccgcagcccg ctgtcgctgt attcggaata ccagcggatg
    10441 gagattgagt ttcgcgcacc gcatatcatg cccaccagcc actggcatgg tcaggtcgaa
    10501 gtgaatgtgc ctttcgatgg cgatgtggaa tacctgatca acaatgaaaa agtgaatatc
    10561 aatcagggtc atatcacgct gttctgggcc tgtacaccgc accaactaac agataccgga
    10621 acctgtcaga gcatggcgat ttttaatctg ccgatgcatc tgtttctctc ctggccgctg
    10681 gataaagacc tgattaacca cgtcactcac ggcatggtga tcaaatcact tgccacacag
    10741 caacttagcc cgtttgaagt acgccgctgg cagcaggaat taaacagtcc gaatgagcaa
    10801 attcgccagc tcgccattga tgaaattggc ctgatgctca agcgatttag cctctctagc
    10861 tgggaaccga ttctggtcaa taaaacctcg cgcacacaca aaaacagcgt ctcgcgccat
    10921 gcgcaatttt atgttagcca gatgctgggc tttattgccg aaaactatga tcaggcgctg
    10981 accatcaacg atgtggctga gcacgtcaaa cttaacgcca actatgcaat ggggatattc
    11041 cagcgggtca tgcaattgac gatgaaacag tacattaccg cgatgcgcat caaccacgtt
    11101 cgcgcgttac tgagcgatac cgataaaagt attctcgata ttgccctgac ggcaggcttt
    11161 cgttcaagta gtcgttttta cagcacgttc ggcaaatatg tcggcatgtc gccgcaacaa
    11221 taccgcaaac ttagccaaca gcgccgccag acgtttcccg gctaaagacg atatcagtat
    11281 cagccaaaaa aatagtttag ccgtcgataa taagcagtga aaataattta aaataatcac
    11341 ataacgtatt gtttataaaa ctaaatattt caaaaatgtt tgtttttcac gcgctttaca
    11401 gcccgaaaag gccggaagat acttgcccgc aacgaagatt ccttcataac cgggtaagca
    11461 atgatgaaag tattaattgt tgaaagcgag tttctccatc aagacacctg ggtcggtaac
    11521 gccgttgagc gtctggcaga tgctttaagc cagcaaaatg ttaccgtgat taaatccacc
    11581 tcctttgatg atggttttgc cattctctct tcaaacgaag ccattgactg cctgatgttc
    11641 agctatcaaa tggagcatcc ggacgaacat caaaacgtca gacaattgat cggtaagctt
    11701 catgagcgcc aacaaaacgt gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc
    11761 gcaatggacc gggatctgct ggagcttgtc gatgaattcg cctggattct ggaagatacc
    11821 gccgacttta tcgccggacg cgccgttgcc gcgatgaccc gctaccgcca gcagctgttg
    11881 ccgccactgt tcagcgcgct gatgaaatat agtgacatcc atgaatattc ctgggcagcg
    11941 ccaggccacc agggcggcgt tggttttacc aaaacacccg ccggacgttt ctaccatgac
    12001 tactatggtg aaaatctgtt ccgtaccgac atgggcatcg aacgaacttc cctcggttct
    12061 ttgcttgacc atactggcgc atttggcgaa agcgaaaaat atgccgcacg cgtatttggt
    12121 gccgatcgct cctggtcggt agtcgtcggt acttccggct ctaaccgcac catcatgcag
    12181 gcttgcatga ccgataacga tgtcgtggtc gttgaccgta actgccataa atccatcgaa
    12241 caaggtttga tgctgacagg cgcgaaaccg gtctatatgg tgccaagccg caaccgctac
    12301 ggcattatcg ggccaatcta tccgcaggaa atgcaacctg aaaccttgca aaagaaaatc
    12361 agtgaaagcc cgctgaccaa agacaaagcc gggcaaaaac cgtcttactg cgtggtaacc
    12421 aactgcacct atgacggcgt gtgttataac gctaaagaag cgcaggatct gctggaaaaa
    12481 acctccgatc gtctgcactt tgacgaagcc tggtacggct atgcacgttt caacccgatc
    12541 tatgccgatc actatgccat gcgcggcgaa cctggcgatc acaacggtcc taccgttttc
    12601 gccacccact ccatccacaa actgctgaat gcgctgtcac aggcttctta tattcatgta
    12661 cgtgaaggtc gtggggcgat taacttctcc cgcttcaacc aggcctacat gatgcatgcc
    12721 accacctccc cgctgtatgc catctgcgca tccaacgacg tggcggtgtc gatgatggac
    12781 ggcaacagcg gcctgtcact gacacaggaa gtgattgacg aagcggttga tttccgtcag
    12841 gcgatggcgc ggctatataa agagttcacc gctgacggta gctggttctt caaaccgtgg
    12901 aacaaagaag tcgtcaccga cccacaaacc ggcaaaacct atgactttgc tgacgcacca
    12961 accaaactgc tgaccaccgt tcaggactgc tgggtaatgc atccgggcga aagctggcac
    13021 ggcttcaaag atattccgga taactggagt atgctcgacc caattaaagt cagcatcctt
    13081 gctccgggaa tgggtgaaga tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc
    13141 actgcctggc ttggtcgcca cggcattgtg cctacccgca ccactgactt ccaaattatg
    13201 ttcctgttct ctatgggcgt aacccgtggg aaatggggga ctctggttaa caccctttgc
    13261 tccttcaaac accactatga cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt
    13321 gaacaatatc ctgacactta cgcgaacatg gggattcacg atctgggtga caccatgttt
    13381 gcctggctga aagaaaacaa ccctggcgca cggttgaacg aagcctattc cggcctgccg
    13441 gtggcggaaa tcaccccgcg tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg
    13501 gtatccattg aaaatctgcc aggacgcatc gcggcaaact cagttatccc gtatccgcca
    13561 ggaatcccga tgctgctgtc tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt
    13621 tatttacgct cgctgcaatc ctgggaccac catttcccta gatttgaaca cgaaactgaa
    13681 gggactgaaa ttattgacgg tatttaccac gttatgtgcg tgaaagcgta accactattc
    13741 cgctgaaggc gtaattgttt aaataacatt acgccgcctg gccttaggcc ttttgagtat
    13801 ggcaacgttt tcataaaaat tgctgcaaac aaa
//
LOCUS       DAZPEU010000100        13560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPEU010000100 DAZPEU010000000
VERSION     DAZPEU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.101"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="ACHU2L_002594"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="ACHU2L_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1357404.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACHU2L_002595"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACHU2L_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1357405.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACHU2L_002596"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACHU2L_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1357406.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACHU2L_002597"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACHU2L_002597"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1357407.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="ACHU2L_002598"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="ACHU2L_002598"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1357408.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACHU2L_002599"
                     /pseudo
     CDS             complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACHU2L_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="ACHU2L_002600"
     CDS             complement(5911..6954)
                     /locus_tag="ACHU2L_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1357409.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            complement(7200..7652)
                     /locus_tag="ACHU2L_002601"
     CDS             complement(7200..7652)
                     /locus_tag="ACHU2L_002601"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1357410.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACHU2L_002602"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACHU2L_002602"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR1357411.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACHU2L_002603"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACHU2L_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR1357412.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACHU2L_002604"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACHU2L_002604"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR1357413.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRECRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACHU2L_002605"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACHU2L_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR1357414.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="ACHU2L_002606"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="ACHU2L_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACHU2L_002607"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACHU2L_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR1357415.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..13414)
                     /locus_tag="ACHU2L_002608"
                     /pseudo
     CDS             complement(12893..13414)
                     /locus_tag="ACHU2L_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         3376 a         3517 c         3393 g         3274 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agttgcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtcaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact aatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgccctgac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggctgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacattcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctgccgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacgggatt
//
LOCUS       DAZPEU010000101        13437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPEU010000101 DAZPEU010000000
VERSION     DAZPEU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.102"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(24..278)
                     /locus_tag="ACHU2L_002609"
     CDS             complement(24..278)
                     /locus_tag="ACHU2L_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR1357416.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHU2L_002610"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHU2L_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR1357417.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHU2L_002611"
     CDS             1228..2694
                     /locus_tag="ACHU2L_002611"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1357418.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHU2L_002612"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHU2L_002612"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR1357419.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHU2L_002613"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHU2L_002613"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR1357420.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACHU2L_002614"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACHU2L_002614"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR1357421.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACHU2L_002615"
     CDS             5709..7265
                     /locus_tag="ACHU2L_002615"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1357422.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACHU2L_002616"
     CDS             7347..9161
                     /locus_tag="ACHU2L_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR1357423.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACHU2L_002617"
     CDS             9162..10256
                     /locus_tag="ACHU2L_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR1357424.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACHU2L_002618"
     CDS             10256..11281
                     /locus_tag="ACHU2L_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1357425.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACHU2L_002619"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACHU2L_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR1357426.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACHU2L_002620"
     CDS             complement(12876..13220)
                     /locus_tag="ACHU2L_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR1357427.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         3230 a         3327 c         3354 g         3526 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgt
//
LOCUS       DAZPEU010000102        13208 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPEU010000102 DAZPEU010000000
VERSION     DAZPEU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.103"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHU2L_002621"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHU2L_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHU2L_002622"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHU2L_002622"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR1357428.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHU2L_002623"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHU2L_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACHU2L_002624"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACHU2L_002624"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR1357429.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACHU2L_002625"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACHU2L_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR1357430.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="ACHU2L_002626"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="ACHU2L_002626"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR1357431.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="ACHU2L_002627"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="ACHU2L_002627"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR1357432.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACHU2L_002628"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACHU2L_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR1357433.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACHU2L_002629"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACHU2L_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR1357434.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACHU2L_002630"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACHU2L_002630"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR1357435.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8149..9470)
                     /gene="astB"
                     /locus_tag="ACHU2L_002631"
                     /pseudo
     CDS             complement(8149..9470)
                     /gene="astB"
                     /locus_tag="ACHU2L_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9467..10945)
                     /gene="astD"
                     /locus_tag="ACHU2L_002632"
     CDS             complement(9467..10945)
                     /gene="astD"
                     /locus_tag="ACHU2L_002632"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR1357436.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10942..11976)
                     /gene="astA"
                     /locus_tag="ACHU2L_002633"
     CDS             complement(10942..11976)
                     /gene="astA"
                     /locus_tag="ACHU2L_002633"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR1357437.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11973..13193)
                     /gene="astC"
                     /locus_tag="ACHU2L_002634"
                     /pseudo
     CDS             complement(11973..13193)
                     /gene="astC"
                     /locus_tag="ACHU2L_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
BASE COUNT         3285 a         3591 c         3182 g         3150 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc ctccctcgcg
     8161 ctggaacgga taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg
     8221 cccttcgcgc agcaattgcg ggtcggccag atcggcagca gtaagacgat cgcggtagta
     8281 acgatccacc cagtcattga gcgcattaaa caaggtatcg ttcatcatca ccgccggatt
     8341 caccgcccgg cgttcttctt ctgtcaatac cacccgcaac cgcaggcacg ccgggccgcc
     8401 gccattcgcc atgctttcac gtaagtcaaa gacttttagt tcgctaatcg ggttgtcagc
     8461 ggcaaggagt tcattgagat aaccccatac tccggcgtgt tcccgacact cctgaggcag
     8521 caccaacatc atggaaccat catcgcggct caacagttgg ctgttaaaca gataggtaga
     8581 caccgcatcc gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac
     8641 ccgcgtgcca gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg
     8701 cggttactca cggcaatcac gtcattatga aaaacgctct ggtcgataac gtccgggttt
     8761 tgctgggcga aaatcacctg ttggggattc acctgattaa gccttgccac cgcctcgctg
     8821 gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg tatcattgcc ttcttctcgc
     8881 ccgtagacaa aaagtagcat acccggttca ccgtaatgac cgccgagacg attgtggttt
     8941 gccgccccct catcaccgag caacgctacc tgcggcaacg ccgaatggac gctaaatttc
     9001 tcttcgtcct taaaaatcgc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg
     9061 tgaaatttat tgttcaggtt ggcaacggtg agatgcactt tgccatccag cgtatcggca
     9121 gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggaact gacgctggaa
     9181 agccagtgcg gtgcctggcg tgcaactttt tccagtacct gctcatcgct accactgaat
     9241 cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcggaatcac ggcctggggg
     9301 aatcccgcat cggcaagggc tttcattttc agtaagccct gttttgccgc cagtcgcggg
     9361 ttagacacct gaaaacggtg acgggtagag gcttcattac caaacgacag gcccgcgtaa
     9421 tgatgcgtca gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct
     9481 catcggaaaa atccagcccg gggttaagcg tggcgggcaa tgttaacgag tccgactcca
     9541 ggctcgccat aggccatgcg cagtaatctg cggcatacca ggcgctgggg cgatggttac
     9601 cggaagcacc aatgccgccg aatggggcgg tactggcagc accagtaagc ggtttgttcc
     9661 agttaacaat ccccgcccgc gcctccagca acagttgatc gaatttttcc cgctcggggg
     9721 aaaccagacc gcaaaagagt ccgaagcgag tgttgttcgc catccgaatc gcctcatcaa
     9781 aagtatcata acgccagacg cgcaaaaacg gcccgaacac ctcttcatcc ggcacaccgc
     9841 caacgcccgt catttcaatg atccctggtg tcagcaacga tgtccctgct tgtaataagc
     9901 gcggcgcaag cagggttcgt ccgcccatcg cttccagttg ctgccaggca gtaaccacct
     9961 gctgtgcggc ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt
    10021 tgcccggcgt taatcgctgg ctgacggcaa ccagactagc aagaaacgca tcgccctgcg
    10081 ctccgctttt gagcaataaa cggcgggcgc aggtgcagcg ttgcccggct gtgacaaacg
    10141 ccgactgaat ggtcagatgg acagccgcgt cgatatccgc cacctcatcg ataattagcg
    10201 gattattacc gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat
    10261 gcagctggta gccggtattg gcgctaccgg taaacagcaa accgtcgaga tcctccagcg
    10321 cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag gttcagcacg cccggcggca
    10381 agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg
    10441 gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat
    10501 gaccagggaa attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc
    10561 tcgccgcacc gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa
    10621 ttgatatcgc gattttattg atcatcgccg tcacttcggt tgccgcttcc cagcgcggct
    10681 tacccgtttc tctggcaata atcgcggtta attcggcttt attgctttcc agcaatccgg
    10741 caaagcgttc gacaacgacc tgacgttcag ccaatgagag ccgcgcccag cgcggaaacg
    10801 ccgcacgggc tgcccgacaa gcctgttcga cctgagcggc atcggcatca ttgccttgcc
    10861 ataacacctc gcccgatacc ggattacgct tcacacgcga tgcgccctgg cccgttatcc
    10921 agtcaccgtt aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg
    10981 aacgcgatcc cctgcgtggc atttgagggc atccagttgt gcggcggtta aaatcaaacg
    11041 ctcggttgcc ggatcggtac gcgccagcac cacgcggaaa tggtgataat tttcattggc
    11101 gaccaggcag gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg
    11161 acttttacgg atggcgcgca cgcggtcgat gtcacactca agcgttggcc cgccgtcgaa
    11221 gatgtcgata tagttgcggt aacgaaaacc ttctttctcc agcaccgcgc gggcaggcgc
    11281 ggtttgcgga tgtacctgac cgatgacgtc ctgggcttcc tgggataaaa agtgggtata
    11341 gatcggatgt ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa
    11401 atcggcgcgg ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga
    11461 atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc
    11521 gcgaaaagcc gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt
    11581 gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact
    11641 aagaaacagc gtcggcaatg cgttatagac attcagctct tttgaggcgt gaaccaacgt
    11701 gccgacgcga tagttgtacc agggatcgtt cagcccaacc gccacctcaa tagcgcaaat
    11761 ccccgccacg gtgcctgtct cgctatcttc cagcacgaac acatagccct gctcgctttt
    11821 gggcagttca ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc
    11881 attggcggga agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga
    11941 gacatctgag cgctcaacgg gacggatgac catcatgatg aacctcggct aacaaagtgt
    12001 tcgcaagcag ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc
    12061 gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta ccatcacgcc tgctttcgcc
    12121 gcttccagag agatctgttt cgcttgcccg gcgtaatcgg cattcagtac acagccaatc
    12181 aacaaaccta agccgcgaac ttcactgaac aaaccatagc ggtgattaat agtattaaga
    12241 cgctcaacaa accagtcgtg acgctgttta acgccattaa gcatctctgg tgtgttgatg
    12301 agctccagca ctttgcctgc taccgccgag gccagcggat taccgccata ggtggtgcca
    12361 tgagtgccaa cggtcatcac gcgggcgcac tcttcggttg ccaacaacgc accgaccggg
    12421 aaaccgccgc ccagcgcttt ggcggtggtt aacagatcgg gcgtcacgcc gtagtgcata
    12481 taggcataca gctcaccagt gcgcccgacg ccggtttgta cctcatcaaa aatcagcaag
    12541 gcattgtggc ggtcacacaa ttcacgcaga ccttgtaaaa acgcgttgct ggctggcacc
    12601 acaccgcctt cgccctagat gggctcgaca atcaccgcac aggtggcgtc gtcaatcagc
    12661 gcgctggcag agttaatatc gttatatgca gcatgacgaa tatccgccgg cagtggcgca
    12721 aaatcctgtg aataggctgg ctgcccaccc gcactgacag taaacagcgt gcgaccatga
    12781 aacgcatttt tgaacgccac gatgccactc ttatggctgc cgtagcggtc gtgagcgaat
    12841 ttacgcgcca gttttagcgc cgcttcgttg gcttccgcac cggagttaca aaagaagacg
    12901 cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg
    12961 taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcttc acgcagttcc
    13021 ggatgcgcat ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc
    13081 ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaaggg tgccggagcg
    13141 taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc
    13201 tctacagt
//
LOCUS       DAZPEU010000103        13098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPEU010000103 DAZPEU010000000
VERSION     DAZPEU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.104"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHU2L_002635"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHU2L_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR1357438.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACHU2L_002636"
     CDS             1131..1931
                     /locus_tag="ACHU2L_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1357439.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACHU2L_002637"
     CDS             2009..2779
                     /locus_tag="ACHU2L_002637"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1357440.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHU2L_002638"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHU2L_002638"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR1357441.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHU2L_002639"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHU2L_002639"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR1357442.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITIPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACHU2L_002640"
     CDS             5034..5756
                     /locus_tag="ACHU2L_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1357443.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHU2L_002641"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHU2L_002641"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1357444.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHU2L_002642"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHU2L_002642"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR1357445.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACHU2L_002643"
     tRNA            7149..7225
                     /locus_tag="ACHU2L_002643"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACHU2L_002644"
     CDS             complement(7356..8402)
                     /locus_tag="ACHU2L_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR1357446.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHU2L_002645"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHU2L_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHU2L_002646"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHU2L_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR1357447.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHU2L_002647"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHU2L_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR1357448.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACHU2L_002648"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACHU2L_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHU2L_002649"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHU2L_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3008 a         3393 c         3483 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 atggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DAZPEU010000104        13015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPEU010000104 DAZPEU010000000
VERSION     DAZPEU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.105"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..319)
                     /locus_tag="ACHU2L_002650"
     CDS             complement(<1..319)
                     /locus_tag="ACHU2L_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFR1357449.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="ACHU2L_002651"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="ACHU2L_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="ACHU2L_002652"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="ACHU2L_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR1357450.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="ACHU2L_002653"
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="ACHU2L_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR1357451.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="ACHU2L_002654"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="ACHU2L_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR1357452.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="ACHU2L_002655"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="ACHU2L_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR1357453.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="ACHU2L_002656"
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="ACHU2L_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR1357454.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="ACHU2L_002657"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="ACHU2L_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="ACHU2L_002658"
     CDS             10613..11503
                     /locus_tag="ACHU2L_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR1357455.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="ACHU2L_002659"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="ACHU2L_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR1357456.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12493..12569)
                     /locus_tag="ACHU2L_002660"
     tRNA            complement(12493..12569)
                     /locus_tag="ACHU2L_002660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13015
                     /locus_tag="ACHU2L_002661"
     CDS             12812..>13015
                     /locus_tag="ACHU2L_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR1357457.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3186 a         3376 c         3304 g         3149 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacacc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAZPEU010000105        13000 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPEU010000105 DAZPEU010000000
VERSION     DAZPEU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.106"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(61..207)
                     /gene="ygdT"
                     /locus_tag="ACHU2L_002662"
     CDS             complement(61..207)
                     /gene="ygdT"
                     /locus_tag="ACHU2L_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR1357458.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            393..1082
                     /gene="mutH"
                     /locus_tag="ACHU2L_002663"
     CDS             393..1082
                     /gene="mutH"
                     /locus_tag="ACHU2L_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR1357459.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1151..1864
                     /locus_tag="ACHU2L_002664"
     CDS             1151..1864
                     /locus_tag="ACHU2L_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1357460.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2002..2220
                     /gene="ygdR"
                     /locus_tag="ACHU2L_002665"
     CDS             2002..2220
                     /gene="ygdR"
                     /locus_tag="ACHU2L_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR1357461.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2328..3368
                     /locus_tag="ACHU2L_002666"
     CDS             2328..3368
                     /locus_tag="ACHU2L_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR1357462.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3401..4591)
                     /gene="lplT"
                     /locus_tag="ACHU2L_002667"
     CDS             complement(3401..4591)
                     /gene="lplT"
                     /locus_tag="ACHU2L_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR1357463.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(4584..6743)
                     /gene="aas"
                     /locus_tag="ACHU2L_002668"
     CDS             complement(4584..6743)
                     /gene="aas"
                     /locus_tag="ACHU2L_002668"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR1357464.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7329..8360
                     /gene="galR"
                     /locus_tag="ACHU2L_002669"
     CDS             7329..8360
                     /gene="galR"
                     /locus_tag="ACHU2L_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR1357465.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8367..9629)
                     /gene="lysA"
                     /locus_tag="ACHU2L_002670"
     CDS             complement(8367..9629)
                     /gene="lysA"
                     /locus_tag="ACHU2L_002670"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR1357466.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9751..10686
                     /gene="lysR"
                     /locus_tag="ACHU2L_002671"
     CDS             9751..10686
                     /gene="lysR"
                     /locus_tag="ACHU2L_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR1357467.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10673..11365)
                     /gene="ygeA"
                     /locus_tag="ACHU2L_002672"
     CDS             complement(10673..11365)
                     /gene="ygeA"
                     /locus_tag="ACHU2L_002672"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR1357468.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11494..12912)
                     /gene="araE"
                     /locus_tag="ACHU2L_002673"
     CDS             complement(11494..12912)
                     /gene="araE"
                     /locus_tag="ACHU2L_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR1357469.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3309 a         3490 c         3185 g         3016 t
ORIGIN      
        1 taatgaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga
       61 tcaaccaatg ccgtgtttat ccacagaatg tgccactaag ttaagcactg aaccactaaa
      121 aactggagtt tcgtcgcacg tcaaggctgt aaatgaaaac agtagtggag gtttttcaca
      181 gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga
      241 tttggaaaac ctattgcagt gttgcgcaac tcgattaccg gcagcctaaa aagcaatata
      301 aatcagagaa ttgaacaaca catgtggaaa aagttacact gcgaatattc ggcacataat
      361 tgctgtttgt tttttaacca aggtatcatg acatgtccca acctcgccca ctgctctctc
      421 ctcccgaaac tgaagaacag ttgttagcgc aagcacagca actttctggt tatacattgg
      481 gagaactggc ggcacttgac gggctggtta cgccagagaa tttaaaacgc gataagggct
      541 ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa cctgagcaag
      601 attttgctgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc
      661 tggaaacaac attcgtttgt gttgcccggt taacgggtaa tagcggggtg acctgggaaa
      721 ccagccatgt gcgccacaag ctcaaacgcg tactgtggat accggttgaa ggcgagcgca
      781 gcatcccgct ggcgcagcgt cgcgtaggat caccgttgct gtggagcccg aatgaagagg
      841 aagaccgaca gctacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcagg
      901 ttgagcggat caccgctcgt cacggggaat atttacagat aagaccgaaa gcagcgaatg
      961 cgaaagcgct taccgaagcc attggtgccc ggggcgaacg gattctgacg ctgccacgcg
     1021 gcttttattt gaagaagaat ttcaccagtg cactactggc ccgtcatttt ctgatccagt
     1081 agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag
     1141 ggctttttag atgttatttg catggataac cgatcctaac gcttggcttg cgctcggtac
     1201 gctgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt
     1261 ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat
     1321 ggttatgcgt ctggcgctgc tggcatcaat ctcttgggtt acgcgcctga caaatccgct
     1381 ttttacaata ttcagtcagg aaatttctgc ccgtgatttg attctgcttc tgggtggctt
     1441 gttccttatc tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg
     1501 gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat
     1561 tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc tgtttattat
     1621 gatggcagcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga
     1681 ttttgtcgaa cgccatcctt cggtaaaaat gctggcgctc tctttcctga ttctggtggg
     1741 ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc
     1801 gatgttcttc tctattgcgg ttgaaagcct caacttgatt cgcaacaaaa agaatccgct
     1861 ctgatactcc gtacgctctc ctgcgggagg gcgtactcac tccttttacc ctcacagttt
     1921 aagattttcc tgctttcaag atatataacg tcggtttata aacagactat tatcataggt
     1981 aggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg
     2041 gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg
     2101 attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat
     2161 cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaattat tgaacgttaa
     2221 caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt cccttccctc
     2281 tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg caatatcacc
     2341 gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg acgtttggtg
     2401 aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct cagggcatta
     2461 accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaacg caagggttaa
     2521 ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag ttaattatcg
     2581 cctccaaagt gagcggaccg tcgcgcaata atgacaaggg catccgcccg gatcaggcgc
     2641 tggatcggaa gaatatccgc gaagcgctgc atgacagcct caagcgccta cagactgatt
     2701 acctcgatct ttatcaggtg cactggccgc agcgcccgac caactgcttc ggcaaactcg
     2761 gttatagctg gacggattct gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg
     2821 cagagtacca acgcgcggga aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg
     2881 gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt gtcaccattc
     2941 agaaccctta cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt
     3001 atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacgctg accgggaaat
     3061 atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg ttcacccgct
     3121 atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg
     3181 gccttgatcc tgctcagatg gcgctcgcgt ttgtacgccg tcaaccgttt gttgccagca
     3241 ctctgctggg cgcaaccacg atggatcagc tgaaaactaa catcgaaagt ttgcatctgg
     3301 agttaagcga agacgtatta gctgaaattg aagcggtaca tcaggtttat acttatcctg
     3361 caccataaag attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga
     3421 tccacagcgc cgttattgcc agcgcaaata gcgcaccgaa gccaatgcca atgggcacga
     3481 ccgggatgcc taccattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt
     3541 tttcgccaag gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct
     3601 cctgtagcaa cgcattgagc ggaacgacaa aaaagccccc cagcacgcca atcagcatca
     3661 acaaggcata ggctggcagc agctcgtgtt gcagggagaa aatcggtacc accacgccaa
     3721 tcaaaatccc ggctggcata cagcgtgaca cggtttccag cgtaaccagc ttcgctgccg
     3781 cacctgcgcc aaccacgata ccaatcgcta ccatcgcgtt gagataggtc ggcgtagcgt
     3841 tatcggtaat gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac
     3901 ccgctcccca gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcaccata
     3961 gcgaggtgca ggcattcagg aaactgcggg tcatgttgat gagattccag gactgccccg
     4021 gacgcgccgc cagtttggga atgtagatat tggcaacgac cgcaccaccg taggccagtg
     4081 cgcatgcggc cagagcgacc aggacatgcc agtcagccag cacaccaccg gctacggaac
     4141 cgagtaaaat cgccgctatg gcagaagctt ccattaaacc gttagctttt actaacttac
     4201 tacccgtggt taattcgccg agaataccgt atttcgccgg tgaataggct gcagcaccaa
     4261 cacccaccag cgtatagccg agaaacggat tgataccaaa gcagatactg gctgcgccca
     4321 gcagcttcag gccgttggca aacatcatca cccggccttt ggcgaagcta tccgccacct
     4381 gcccgacaaa cggcgccaaa agaatgtaag cacctacaaa caccatttgc aggatgggct
     4441 ggctccactc cggatagaac tgcgctttca gtaacgccag agtggcaaac agtagcgcgt
     4501 tatcgccaaa cgcagagaga aactgcgcca cgataaccgc tttcatcccc ttcgaccaca
     4561 acgaagtgtt agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc tacccagctt
     4621 ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg gcatctgttt cagatagcga
     4681 atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc gggcatactg ttgcaactta
     4741 tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg
     4801 ctcttaatcg cagtggcatg gactttatct ggcgaaacac caagtgccag ttgttccacc
     4861 atttccagcg acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc
     4921 tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag tgtcatacca gccgcgctcc
     4981 atttcgccgc gaacattctc ggcggtgggc acttccagta cacctggctt ctccacccgc
     5041 agatagccgt tcattatgtt cggccctttc agttgcaggc gtccgccctc ttcgatacca
     5101 gggaccgaca acaggcgtgc atccattcct ggtagaatac gccctaccgt accgggtttc
     5161 gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt cggtcacacc gtagccttca
     5221 aggatgcgca ggccaaattt atcctgccaa agctgtttgg tactttcttg tagtttttct
     5281 gcgcctgcca ccacatagcg tagacgatag aagtcatacg ggttggcgaa acgcgcgtag
     5341 tgaccgagga aagtcgaggt accgaacaac acggtgcaac tgcggtcata caccaagtcc
     5401 ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca cttctgcacc tgtaagcagt
     5461 ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca gcggtaatgc cgacataaag
     5521 cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct cgacgttcgc cagaatgctt
     5581 ttatggctat ggacgacgcc ttttggatgg ccttcagaac cggaggtaaa gaggatcagc
     5641 gcctcttctt ccggctgctg ttttacctgc gccaggcgcg gcatcagcaa atgagcgaag
     5701 atccatactt tgtcggcagt ggtgacatct gcttttaaat cttccagata gacccagcgc
     5761 acctgagtaa gttgctccgg cagatgccag agtttgcctt tatcgagaaa ctggcgggaa
     5821 gtgaagatgg ttttgatttc agccgccgta atagcactgg tcagcccttt taccccggca
     5881 gtgtagttca tcattgcggg aatgcggcga cgcgcgatgg ccccaaaaat cactgccgca
     5941 ctgatgcctg cattgggcag cattaagccg atgcgttcgc cttcaacact gtatttttca
     6001 aggatgcgtc caacaaacag cgttttcgta agcaatttgc gataggagtc tggggtaaag
     6061 ttgacgtctt cgacacattt cttcccggct ccgaagcggt acattgcact cagtaaagat
     6121 tcgtacagag tttcacgcgg gcgcaccgcc atacgcgctt ccatcattat ttgatgcagc
     6181 atttcgccag cgattttgcg acggtcacgg gcacgcggcg catccggcat cgccacctgc
     6241 gttggaggca aaatatgcag agtaatttgc gggaacaagc gacgtttaac cagacctttc
     6301 aggcggctga agtgcgtaag ttccgcccct tcaatacgca caggaataac cgttgcacca
     6361 gacttcgccg cgacaaaacc cgcgccatcg tagattttca tcagcgagcc tgtagtggtg
     6421 atgcgtcctt cagggaaaat caccactggt cggccctgtt ccaccagacg taccagatgt
     6481 ttaatagcca taggttgcgt cgggtcgaga ggaacaaagt cgataaatga tttcagccaa
     6541 cgcatatacc actgttggct tattgaggtg taaacggcaa acactggacg cacaggtaaa
     6601 aacagtccaa gcaaaatgcc atcaataaaa gagacgtgat taggcgtaat tagtacgcgc
     6661 tcgcccttca gtgcctgggt gtcacccgta acgcgaacgc gatacaaaac acggcacaaa
     6721 tttcgaaaaa agctaaaaag cattccaact ccctttgctc tgattcagta aaagcgaatg
     6781 gagggagatt acacgagata aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc
     6841 tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa gagcgtaccg aataatctca
     6901 ccaatcaata cctctgggat cttgattgtg gtctgcacga cgactcttcg ccagcgagaa
     6961 aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga
     7021 ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca
     7081 acacgaagtt gacttcacct atcaatacct ctgggatcac cactttagca acctgaagcc
     7141 aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat
     7201 ctcttttttt gtcttactta tttgcgtttg gctcacattc ccacgatgaa aacacgccac
     7261 cccttgaacc aacgggcgtt ttccgtaaca ctgaaacaat gtaagcgttt acccactaag
     7321 gtattttcat ggcgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg
     7381 tttcccgcgt cattaataat tcacccaaag ccagcgaagc ttcccggctg gctgtgcata
     7441 gtgcaatgga gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga
     7501 ccactgaaac ggtcggtctg atcgttggtg atgtttccga tccgtttttc ggtgcaatgg
     7561 tgaaagcggt cgaacaggtg gcttatcaca ccggtaattt cttattgatt ggcaacggtt
     7621 accacaacga acaaaaagag cgtcaggcta ttgagcaact gatccgccat cgctgtgctg
     7681 cgttggtcgt ccatgccaaa atgatcccag atgccgattt agcctcatta atgaaacaaa
     7741 tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc
     7801 ttgacgatcg ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc
     7861 gcattggtta tctgtgctct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt
     7921 attacgatgc ccttgctgaa agtggtattc cggccaatga tcggctggtg acatttggcg
     7981 aaccagacga aagcggcggc gaacaggcaa tgaccgagct tttgggacga ggaagaaatt
     8041 tcactgcggt agcctgttat aacgattcaa tggcggcggg cgcgatgggc gttctcaatg
     8101 ataatggtat tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt
     8161 cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtaacg atggcgaccc
     8221 aggctgccga actggctttg gcactggcgg ataatcgccc tctcccggaa attactaatg
     8281 tatttagtcc gacgctggta cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc
     8341 atcatgcaac cagcgactaa ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg
     8401 gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc
     8461 ggacggctat tgtagttgga tgacattgat gcgccatatg cccctgtatc atgcagtacc
     8521 agataatcac ctgccttcac ttccggcaag gcgcgggttt caacatttcc cccttcctgc
     8581 tgggtaaaga catcgcccga ttcacataac ggcccggcga cgacggtttc caccgttggc
     8641 gcgtgttcca gagaacgacc atcagctgcc agggcactga tatggtggta actaccgtac
     8701 attgccgggc gcatcagatc gttgaacccg gcatcaacca gcacaaagtg gcggctaccc
     8761 atttgtttga cgctccgcac ctgagtaatt aatacgccag actgcgctac caggaagcga
     8821 cccggttcaa tttccagttt cacagggtgg cccaaatggc gagcgatttg ctcacgcgcg
     8881 gcattccaca gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga
     8941 atagaaagcc caccgcccgc agaaatagcc tgtaaatcct gaccgaattc gataacctga
     9001 cgcaccatag caccacacac ctgctccaga tgggcataat caacgccaga accaatgtgc
     9061 atgtgaatgc cgactagctg cagatgatga cgttgtatca cgtccagtgc ggcgggcaga
     9121 tcggtgtacc agataccgtg cttgctgttt tcgccaccgg tattggtttt ttggctatgt
     9181 ccgtgaccaa accccggatt aacgcgcagc catacccgat gccctggcga aacctggccc
     9241 agttggtcga gcgtatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt
     9301 tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc
     9361 ggattgtaac ccgccgccaa cgcacgctct atttcgccta acgagacgga atccactttc
     9421 acgccctgct cacgcattaa gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag
     9481 cgcaccactt caaactgttt cagcgctgca atctgccgac gaataatttg cgcatcgtag
     9541 acccacaccg ggcagccaaa ttcagcgggc aaacgcagca gattttcggc ggtgagatcg
     9601 gtatcggtgc tgaacagtga atgtggcata acaaactcca gatgagtgct tttttatgat
     9661 tacgccacat cataaaaaga ataaaaaata tcgatttatg tcgagtctat gcaaaaatga
     9721 tatggattac cggattgcga gagagcgcta atggccgccg ttaacttacg tcatattgaa
     9781 atttttcatg cggtaatgac cgccggaagc ctgactgagg cagcacacct gctacacacc
     9841 tcacagccaa ccgtcagccg cgaactggcg cgctttgaga aggtgatcgg gctgaaattg
     9901 tttgagcgcg tacgtggacg attacatcct accgtgcaag gactgcgtct gtttgaagaa
     9961 gtgcaacgat cctggtacgg actggatcgc attgtcagcg ccgcagaaag tctgcgcgag
    10021 tttcgccagg gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg
    10081 cagctcctgc aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag
    10141 gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgag
    10201 acactccata cgcctacggg aacagaacgt accgaattac tctctttaga tgaagtgtgt
    10261 gtgttacctc cgggtcatcc gctggcggta aaaaaggtat taacgccgga tgattttcag
    10321 ggtgagaatt acatcagcct ttcccgtact gacagctatc gccagttgct ggatcagcta
    10381 tttactgaac atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc
    10441 tgcgcaatgg tacgggcggg ggtaggtatt tcggtggtta acccgctcac cgcactagat
    10501 tatgcggcaa gcggtttagt ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc
    10561 ctgatccgcc ccctgcaccg cccgtcatca gcgctggttc aggcgtttag tgggcattta
    10621 caagcggggt taccgaaact ggtcacttct cttgacgcta ttttgtcgtc agctacgaca
    10681 gcataaaagc gacagcatcc tcggcatgga tcgccgcggt atcaaacaca ggcaaaacac
    10741 tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc
    10801 cctgttctgc aaggcgagca atcacttgcg cataataagc gcgtgacgct tcggtaaatt
    10861 gccccagaca cagttcttca aaaataatct gattaatttt cgcccgttca tccgcttcag
    10921 gaataagaca attgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca
    10981 tggtgtaacg cgtacccagc agcgccacac gagtcattcc ggccccggta attgcacgtc
    11041 cggtggcatc cgcaatgtgt aagaaaggca gagtgcaacg tgactcaatg gcatccgcca
    11101 ctttatgcat cgtattggta cataacacaa taccttctgc gcccgcccgc tgtaagccaa
    11161 gcgccgcctc agccagaatg tccccggttt tatcccattc cccgcgacgc tggcactctt
    11221 ctatttcatg aaaatcgacg ctatgtagca gcatttgcgc agagtgaagc ccaccaagcc
    11281 gctgtttaat gccttcattt atcagacgat agtaaggaat ggtggattcc cagctcattc
    11341 ctcccagcaa accaattgtt ttcattcctc tctcctgatg tgtgttaccg caacaaggct
    11401 gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc aatctctatt aacgaaaaaa
    11461 gggccggatg tacagcacat ccggcccgtg aaatcagacg ccgatatttc tcaacttctc
    11521 gcctgccatc agtttgcgtt cgatatgttc cagcgtgaca tttttggttt ccggaatgag
    11581 ccagaaagta atacccacaa acgcaatgtt cagcgcagtg tagagccaga acgtaccggc
    11641 agcgccaatg ctatcaagca gtgtcaggaa ggccgcgccg ataatcatat tcgacaccca
    11701 gtttgtcgtc gtcgaacagg taataccgaa atcgcggcat ttcagcggct gaatttcaga
    11761 gcacaggatc cacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt
    11821 catgccaaca gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag
    11881 gcaatagccc agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagagccgg
    11941 tttacgacct gctttatcta ccgtaaagac cgcaatgaag gtcgcgaaca taaaggtcag
    12001 tcccacgacc agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa
    12061 gatacgcggc gcgtagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa
    12121 caacataccg agaaacacag cacgacggac gttacggttg atcttaaaca gtgcccaacc
    12181 gccctgtttt aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg ctttttccga
    12241 cgtatcgcgc agcatacgca acacttcttc cgcctcaata tgacgcccct tttccgccag
    12301 ccagcgcggg ctatttggca ggaagaccac cagaataatc agcagaactg ctggtaaagc
    12361 aagaaccccc aacattgcgc gccagttacc gctataactg aacgctgtat cggataaaaa
    12421 cgccagcacg atgccgagtg tgaccatcaa ctggtacata ctgatcatct taccgcgaac
    12481 gttttcactt gccatttcag aaagatacag aggagcggtg taagacgcga tcccgacagc
    12541 aatgcccagc accacacgag cggcgattaa catctctacg ctggtcgcaa aagcggaccc
    12601 tatagaaccg agtacaaaca ggatggcccc cgccatcagg ctgtatttac gccccaggcg
    12661 gaacgacagc caaccattaa acagcgcacc aattgctgcg ccgagcatca tgctactaac
    12721 cacccattcc tgcaaacgac tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc
    12781 ggcgattacg ccgatatcaa gaccaaataa caatcctgcg accgcagcag ctaccgaaac
    12841 aaacatattc atacgccgcg tatcacgcaa agaacgtggc gttaaagcag attccgtatt
    12901 gatagtaacc attttttcct gccagcagag agtaagacat tgtgaaaaaa tacgtgaaca
    12961 attcacacag gtgtcaggtc ggaaacagca taaatatgga
//
LOCUS       DAZPEU010000106        12857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPEU010000106 DAZPEU010000000
VERSION     DAZPEU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.107"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="ACHU2L_002674"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="ACHU2L_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR1357470.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="ACHU2L_002675"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="ACHU2L_002675"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR1357471.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="ACHU2L_002676"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="ACHU2L_002676"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR1357472.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="ACHU2L_002677"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="ACHU2L_002677"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR1357473.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="ACHU2L_002678"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="ACHU2L_002678"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR1357474.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="ACHU2L_002679"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="ACHU2L_002679"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR1357475.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="ACHU2L_002680"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="ACHU2L_002680"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR1357476.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="ACHU2L_002681"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="ACHU2L_002681"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR1357477.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /gene="hisIE"
                     /locus_tag="ACHU2L_002682"
     CDS             7140..7751
                     /gene="hisIE"
                     /locus_tag="ACHU2L_002682"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR1357478.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="ACHU2L_002683"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="ACHU2L_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR1357479.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="ACHU2L_002684"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="ACHU2L_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="ACHU2L_002685"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="ACHU2L_002685"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR1357480.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11945..12259)
                     /locus_tag="ACHU2L_002686"
     CDS             complement(11945..12259)
                     /locus_tag="ACHU2L_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357481.1"
                     /translation="MKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDW
                     TISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIA
                     IK"
     gene            complement(12301..>12857)
                     /locus_tag="ACHU2L_002687"
     CDS             complement(12301..>12857)
                     /locus_tag="ACHU2L_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357482.1"
                     /translation="ILIAKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGGN
                     IFGHYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKI
                     LWVFFSIVLTYVLFWFSVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYF
                     ILVCIIFFRGDRCILNSNIFILNT"
BASE COUNT         3315 a         3224 c         3159 g         3159 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt
    11881 atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg
    11941 cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg
    12001 gatacttttc tggattaaaa ttagcatagc ttcgtttaga tataacaata aacgaactat
    12061 taaaagattt catcttctct attaaatcat cctgtgggat tacctcacac tcgggattac
    12121 taatagtcca atcactatat cttgctaatc caaagacgtc accaattgcc atccctttat
    12181 agaagtaaat accatcctcc aagcctaatt ggataagatt atctccatat aactcactat
    12241 attcacttgc tttcttcatc agttcatagc tatagcgact ttctaagcgt gaattaaaat
    12301 tcaagtattc agaataaaaa tatttgaatt caaaatacaa cgatcgcccc gaaaaaaaat
    12361 aatacacaca aggataaaat atcgtcccaa cttgtaattt gtaatgttga tatcttttat
    12421 taaacttgca attgagatcg caacctgcaa tactgacaat gggtaaatgg gtgataaata
    12481 cctgtcacct tgcgtaacag aaaaccaaaa taagacatat gttaaaacaa tcgagaaaaa
    12541 tacccaaagt attttattat tcctaagtgt aaagacagac agtatagcat aaaaaacaac
    12601 atcactgcca actttattaa ttgcttgttg caaatctaat gtggcaggac ttataccatg
    12661 tcgagcttgt tctgctgttt gaacttgtag atcaatttca ttccataggt agtgaccaaa
    12721 tatatttcct ccagcaggac taaaaggatc accagagatc caaaaactac ggacatacca
    12781 ataaatgcca ccaagaattg ttgccaagct aaaaataaga aatgtctgta tgtttctctt
    12841 tttcgctatc aatataa
//
LOCUS       DAZPEU010000107        12837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPEU010000107 DAZPEU010000000
VERSION     DAZPEU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.108"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1137
                     /gene="proV"
                     /locus_tag="ACHU2L_002688"
     CDS             <1..1137
                     /gene="proV"
                     /locus_tag="ACHU2L_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR1357483.1"
                     /translation="KYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSG
                     KSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVL
                     DNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAIN
                     PDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQN
                     GEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRS
                     ALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPL
                     SELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1130..2194
                     /gene="proW"
                     /locus_tag="ACHU2L_002689"
     CDS             1130..2194
                     /gene="proW"
                     /locus_tag="ACHU2L_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR1357484.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2252..3244
                     /gene="proX"
                     /locus_tag="ACHU2L_002690"
     CDS             2252..3244
                     /gene="proX"
                     /locus_tag="ACHU2L_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR1357485.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3436..4615
                     /locus_tag="ACHU2L_002691"
                     /pseudo
     CDS             3436..4615
                     /locus_tag="ACHU2L_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4739..5476
                     /gene="ygaZ"
                     /locus_tag="ACHU2L_002692"
     CDS             4739..5476
                     /gene="ygaZ"
                     /locus_tag="ACHU2L_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR1357486.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5466..5801
                     /gene="ygaH"
                     /locus_tag="ACHU2L_002693"
     CDS             5466..5801
                     /gene="ygaH"
                     /locus_tag="ACHU2L_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR1357487.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5892..6422
                     /gene="emrR"
                     /locus_tag="ACHU2L_002694"
     CDS             5892..6422
                     /gene="emrR"
                     /locus_tag="ACHU2L_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR1357488.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6549..7721
                     /gene="emrA"
                     /locus_tag="ACHU2L_002695"
                     /pseudo
     CDS             6549..7721
                     /gene="emrA"
                     /locus_tag="ACHU2L_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            7738..9276
                     /gene="emrB"
                     /locus_tag="ACHU2L_002696"
     CDS             7738..9276
                     /gene="emrB"
                     /locus_tag="ACHU2L_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR1357489.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9340..9855)
                     /gene="luxS"
                     /locus_tag="ACHU2L_002697"
     CDS             complement(9340..9855)
                     /gene="luxS"
                     /locus_tag="ACHU2L_002697"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR1357490.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10005..11561)
                     /gene="gshA"
                     /locus_tag="ACHU2L_002698"
     CDS             complement(10005..11561)
                     /gene="gshA"
                     /locus_tag="ACHU2L_002698"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR1357491.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(11634..12062)
                     /locus_tag="ACHU2L_002699"
     CDS             complement(11634..12062)
                     /locus_tag="ACHU2L_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR1357492.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12059..12625)
                     /gene="yqaB"
                     /locus_tag="ACHU2L_002700"
     CDS             complement(12059..12625)
                     /gene="yqaB"
                     /locus_tag="ACHU2L_002700"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR1357493.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         2958 a         3394 c         3305 g         3180 t
ORIGIN      
        1 aaatatatcg aacaaggact ttcaaaagaa caaattctgg aaaaaactgg gctatcgctt
       61 ggcgtaaaag acgccagtct ggccattgaa gaaggcgaga tatttgtcat catgggatta
      121 tccggctcgg gtaaatccac aatggtacgc cttctcaatc gcctgattga acccacccgc
      181 gggcaagtgc tgattgatgg tgtggatatt gccaaaatat ccgacgccga actccgtgag
      241 gtgcgcagaa aaaagattgc gatggtcttc cagtcctttg ccttaatgcc gcatatgacc
      301 gtgctggaca atactgcgtt cggtatggaa ttggccggaa ttaatgccga agaacgccgg
      361 gaaaaagccc ttgatgcact gcgtcaggtc gggctggaaa attatgccca cagctacccg
      421 gatgaactct ctggcgggat gcgtcaacgt gtgggattag cccgcgcgtt agcgattaat
      481 ccggatatat tattaatgga cgaagccttc tcggcgctcg atccattaat tcgcaccgag
      541 atgcaggatg agctggtaaa attacaggcg aaacatcagc gcaccattgt ctttatttcc
      601 cacgatcttg atgaagccat gcgtattggc gaccgaattg ccattatgca aaatggtgaa
      661 gtggtacagg tcggcacacc ggatgaaatt ctcaataatc cggcgaatga ttatgtccgt
      721 accttcttcc gtggcgttga tattagtcag gtgttcagtg cgaaagatat tgcccgccgg
      781 acaccgaatg gcttaattcg taaaactcct ggcttcggcc cacgttcggc actgaaatta
      841 ttgcaggatg aagatcgcga atatggctac gttatcgaac gcggtaataa gtttgtcggc
      901 gcagtctcca tcgattcgct taaaaccgcg ttaacgcagc agcaaggtct tgatgcggcg
      961 ctgattgatg cgccgttagc agtcgatgca caaacgcctc ttagcgagtt gctctctcat
     1021 gtcggacagg caccctgtgc ggtgcccgtg gtcgacgagg accaacagta tgtcggcatc
     1081 atttcgaaag gaatgctgct gcgcgcttta gatcgtgagg gggtaaataa tggctgatca
     1141 aaataatccg tgggatacca cgccagcggc ggacagtgcc gcgcaatccg cagacgcctg
     1201 gggtacaccg acgactgcac cggctgacgg cggtggtgct gactggctga ccagtacgcc
     1261 tgcgccaaac gtcgagcatt ttaatattct cgatccgttc cataaaacgc tgatcccgct
     1321 cgacagttgg gtcactgaag ggatcgactg ggttgttacc catttccgtc ccgtcttcca
     1381 gggcgtgcgc gttccggttg attatatcct caacggtttc cagcaattgc tgctgggtat
     1441 gccagcgcca gtggcgatta tcgttttcgc tctcatcacc tggcagattt ccggggtcgg
     1501 aatgggcgtg gcgacgctgg tctcgctgat tgccatcggc gcaatcggtg cctggtcgca
     1561 ggcaatggtg actctggcgc tggtgttaac tgccctgctg ttctgtatcg tcatcggttt
     1621 gccgttgggg atatggctgg cgagaagtcc gcgagcggcg aaaattattc gtccactgct
     1681 tgatgccatg cagaccacgc cagcgtttgt ttatctggtg ccaatcgtca tgctatttgg
     1741 tatcggtaac gtgccgggcg tggtggtgac gatcatcttt gcgctgccgc cgattatccg
     1801 tctgaccatt ctggggatta accaggttcc ggcggatctg attgaagcct cgcgctcatt
     1861 cggtgccagc ccgcgccaga tgctgttcaa agttcagtta ccgctggcga tgccgaccat
     1921 tatggcgggc gttaaccaga cgctgatgct ggccctttct atggtggtca ttgcctcgat
     1981 gattgccgtc ggcgggctgg gtcagatggt acttcgcggt atcggtcgtc tggatatggg
     2041 gcttgccacc gttggcggcg tcgggattgt gatcctcgcc attatcctcg atcgcctgac
     2101 gcaggccgtt gggcgcgact cacgcagtcg cggcaaccgt cgctggtaca ccactggccc
     2161 tgttggtctg ctgacccgcc cattcattaa gtaactctgc acttgcccgg tgacgccggg
     2221 cattatcacc ctgccaaaaa aaggaataac aatgcgacat agcgtacttt ttgcgacagc
     2281 gtttgccacg cttatctcta cacaaacttt tgctgccgat ctgccgggca aaggcattac
     2341 tgttaatcca gttcagagca ccatcactga agaaaccttc cagacgctgc tggtcagtcg
     2401 tgcgctggag aaattaggtt ataccgtcaa caaacccagc gaagtggatt acaacgttgg
     2461 ctacacctcg cttgcttccg gcgatgcaac cttcaccgcc gtgaactgga cgccactcca
     2521 tgacaacatg tacgaagctg ccggtggcga taagaaattt tatcgtgaag gggtatttgt
     2581 taacggcgca gcacagggtt acctgatcga taagaaaacc gccgaccaat acaaaatcac
     2641 caacatcgca caactgaaag atccgaagat cgccaaactg ttcgatacca acggcgacgg
     2701 taaagcggat ttaaccggtt gtaaccctgg ctggggctgc gaaggtgcga tcaaccacca
     2761 gcttgccgcg tatgaactga ccaacaccgt gacgcataat caggggaact acgcagcgat
     2821 gatggccgac accatcagtc gctacaaaga gggcaaaccg gtgttttatt acacctggac
     2881 gccgtactgg gtgagtaatg agctgaagcc agggaaagat gtggtctggt tgcaggtgcc
     2941 gttctccgca ctgccgggcg ataaaaatgc cgataccaaa ctgccgaatg gcgcgaatta
     3001 cggcttcccg gtcagcacca tgcatatcgt tgccaacaaa gcctggaccg agaaaaatcc
     3061 ggcggcagcc aaactgtttg ccattatgca gttgccagtg gccgatatta acgcccagaa
     3121 cgccattatg catgacggca aagcctcaga aggcgatatt cagggccacg ttgatggctg
     3181 gatcaaagcc caccagcagc agttcgatgg ctgggtgaat gaggcgctgg cagcgcagaa
     3241 gtaattttta ttcgggcgga taaggcgttt tcgcctcatc cgccgctctg tacacaatgc
     3301 ctgatgcgac gctgacgcgt cctatcatgc ctacaaacca catcgcacat ttcaacaagc
     3361 tatctttcat cccatattca tcaacatccg ctattattga tttccagctt aatcatcacc
     3421 tgatgaacaa aaataatgac taaacctaat catgagctta gcccggcgct gatcgtgctg
     3481 atgtctatcg ccaccggtct ggcggtagcc agtaactatt acgcccagcc attgctcgac
     3541 accatcgcgc gtaacttttc cctttccgcc agttcggcag gctttattgt taccgccgcg
     3601 cagttgggct atgccgcagg tctactgttt cttgttcccc tcggtgatat gtttgaacgc
     3661 cgccgcctga ttgtctcgat gaccttactg gcagcgggcg gtatgttaat cactgccagc
     3721 agtcagtcac tggcgatgat gatcctcggt acggcattaa ccggtttatt ctcagtcgtg
     3781 gcacaaattc tggttccgct ggcagcgacg ctggcttcac cggataaacg cggcaaagtg
     3841 gttggcacca ttatgagcgg tctgctgttg gggatattgc tggcacgaac cgttgccggg
     3901 ttactggcaa acctcggcgg ctggcgcacc gtcttttggg ttgcttcggt gttaatggca
     3961 ctgatggcgc tggcattatg gcgtggtctg ccacaaatga aatcagaaac ccacctcaac
     4021 tacccacagt tgttgggttc cgttttcagc atgttcatca gcgataagat cctgcgcacc
     4081 cgcgcgttgc tgggctgcat gacctttgcc aacttcagca ttctctggac ctcaatggcc
     4141 tttttgcttg ccgctccacc ttttaactac agcgatggtg acattggtct gtttgggctt
     4201 gcgggagctg ccggagcgtt gggcgctcgt ccggcgggcg gttttgccga caagggcaaa
     4261 tcgcaccaca actttcggtc tgcttctgct gttactttca tggctggcaa tctggtttgg
     4321 tcacacttcc gtactggcgt tgattatcgg cattctggtg ctggacctca ccgtacaggg
     4381 cgtgcatatc actaaccaga cggtaattta tcgaatacat cctgatgcgc gtaatcgcct
     4441 gaccgcaggt tacatgacca gctactttat tggcggtgcc gccggttcgc taatttcagc
     4501 ctcagcctgg caacatggcg gttgggctgg cgtttgtctg gctggcgcga cgattgccct
     4561 ggttaactta ctggtctggt ggcgaggttt tcatcgtcag gaagccgcaa attaagcaat
     4621 cagcaaccgt tttcgtggct ttacacttat aagggtgtta agaagcccat cagtctgata
     4681 aggttaagat attcattcag tctatttata atattaacaa tcgttaagcg tacactctat
     4741 ggaaagccct actccacagc ctgctcctgg ttcggcgacc ttcatggaag gatgcaaaga
     4801 cagtttaccg attgttatta gttatattcc ggtggccttt gcgttcggtc tgaatgcgac
     4861 ccgtctggga ttctctcctc tcgaaagcgt ttttttctcc tgcatcattt atgcaggcgc
     4921 gagccagttc gtcattaccg cgatgctggc agccgggagt agtttgtggg ttgctgcact
     4981 gagcgtcatg gcaatggatg ttcgccatgt tttgtatggc ccgtcactgc gtagccgtat
     5041 tattcagcgt ctgcaaaaat cgaaaaccgc cctatgggcg tttggcctga cggatgaggt
     5101 ttttgccgcc gccaccgcaa aactggtacg caataatcgc cgctggagcg agaactggat
     5161 gatcggcatt gccttcagtt catggtcatc gtgggtattt ggtacggtaa taggggcatt
     5221 ctccggcagc ggcttgctgc aaggttatcc cgccgttgaa gctgcattag gttttatgct
     5281 tccggcactc tttatgagtt tcctgctcgc ctctttccag cgcaaacaat ctctttgcgt
     5341 taccgcagct ttagttggtg cccttgcagg cgtaacgcta ttttctattc ccgtcgccat
     5401 tctggcaggc attgtctgtg gctgcctcac tgcgttaatc caggcattct ggcaaggagc
     5461 gcccgatgag ctatgaggtt ctgctgcttg ggttactggt tggcgcggcg aattattgct
     5521 tccgctattt gccgctgcgc ctgcgtgtgg gtaatgcccg cccaaccaaa cgtggcgcgg
     5581 taggtatttt gctcgacacc attggcatcg cctcgatatg cgctctgctg gttgtctcta
     5641 ccgcaccaga agtgatgcac gatacacgcc gtttcgtgcc cacgctggtc ggcttcgcgg
     5701 tactgggtgc cagtttctat aaaacacgca gcattatcat cccaacactg cttagtgcgc
     5761 tggcctatgg gctcgcctgg aaagtgatgg cgattatata acccacaaga atcatttttc
     5821 taaaacaata catttacttt atttgtcact gtcgttacta tatcggctga aattaatgag
     5881 gtcataccca aatggatagt tcgtttacgc ccattgaaca aatgctaaaa tttcgcgcca
     5941 gccgccacga agattttcct tatcaggaga tccttctgac tcgtctttgc atgcacatgc
     6001 aaagcaagct gctggagaac cgcaataaaa tgctgaaggc tcaggggatt aacgagacgt
     6061 tgtttatggc gttgattacg ctggagtctc aggaaaacca cagtattcag ccttctgaat
     6121 taagttgtgc tcttggatca tcccgtacca acgcgacgcg tattgccgat gaactggaaa
     6181 aacgcggttg gatcgaacgt cgtaaaagcg ataacgatcg ccgctgcctg catctgcaat
     6241 taacggaaaa aggtcacgag tttttgcgcg aggttttacc accgcagcat aactgcctgc
     6301 atcaactctg gtccgcgctc agcacaacag aaaaagatca gctcgagcaa atcacccgca
     6361 aattgctctc ccgtctcgac cagatggaac aagacggtgt ggttctcgaa gcgatgagct
     6421 aacgcgtcat ctcgctcaaa aatccagatt tataaaagaa aaaatgaccg gccagcatcg
     6481 caacatgctg gcctttttgg caagcaggtc ggctcagccg atgagttaag aagatcgtgg
     6541 agaacaatat gagcgcaaat gcggagactc aaaccccgca gcaaccggta aagaagagcg
     6601 gcaaacgtaa gcgtctgctc ctccttctca ccttgctctt tataattatt gccgtagcga
     6661 tagggattta ttggtttttg gtactgcgtc atttcgaaga aaccgatgac gcatacgtgg
     6721 cagggaatca gattcaaatt atgtctcagg tgtctggcag cgtgacgaaa gtctgggccg
     6781 ataacaccga ttttgtaaaa gaaggcgatg ttctggtcac tctcgacccg acagatgctc
     6841 gccaggcgtt tgaaaaagcc aaaactgcac tggcttccag cgttcgccaa acccaccagc
     6901 tgatgattaa cagcaaacag ttgcaggcga atattgaggt gcaaaaaatc gccctcgcga
     6961 aagcacaaag cgactacaac cgccgtgtgc cgctgggcaa tgccaacctg attggtcgcg
     7021 aagagctgca acacgcccgc gacgccgtca ccagtgccca ggcgcaactg gacgtcgcta
     7081 ttcaacaata caatgccaat caggcgatga ttctggggac taaactggaa gatcagcctg
     7141 ccgtgcaaca ggctgccacc gaagtacgta acgcctagct ggcgctggag cgtacccgta
     7201 ttgtcagtcc gatgaccggt tatgtttccc gtcgtgccgt gcaaccgggt gcgcaaatta
     7261 gcccaacgac gccgctgatg gcggtcgttc cagccaccaa tatgtgggtg gatgccaact
     7321 ttaaagagac gcagattgcc aatatgcgca tcggtcagcc ggtcaccatc accaccgata
     7381 tttacggcga tgatgtgaaa tacaccggta aagtggttgg tctggatatg ggcacaggta
     7441 gcgcgttctc actgcttccg gcgcaaaatg cgaccggtaa ctggatcaaa gtcgttcagc
     7501 gtctacctgt gcgtatcgaa ctggaccaga aacagctgga gcaatacccg ctgcgcattg
     7561 gtttgtccac gctggtgagc gtcaatacca ctaaccgtga cggtcaggta ctggcaaata
     7621 aagtacgttc aacaccggtc gcggtaagta ccgcgcgtga aatcagcctg gcacctgtca
     7681 ataaactgat cgacgatatc gtaaaagcta acgctggcta atccagaggt gcgtgtgatg
     7741 caacagcaaa aaccgctgga aggcgcgcaa ctggtcatta tgacgattgc gctgtcactg
     7801 gcgacattca tgcaggtgct gaactccacc attgctaacg tggcgatccc cactatcgcc
     7861 gggaatctgg gctcatcgct cagccaagga acgtgggtaa tcacttcttt cggggtggcg
     7921 aatgccatct cgatcccgct taccggctgg ctggcaaagc gcgtcgggga agtgaaactg
     7981 ttcctttggt ccaccatcgc ctttgctatt gcgtcttggg cgtgtggtgt ctccagcagc
     8041 ctgaatatgc tgatcttctt ccgcgtgatt caggggattg tcgccgggcc gttgatcccg
     8101 ctttcgcaaa gtctattgct gaataactac ccgccagcca aacgctcgat cgcgctggcg
     8161 ttgtggtcga tgacggtgat tgtcgcgcca atttgcggcc cgatcctcgg cggttatatc
     8221 agcgataatt accactgggg ctggatattc ttcatcaacg tgccgattgg cgtggcggtg
     8281 gtgttgatga cactgcaaac cctgcgcgga cgtgaaaccc gcaccgaacg gcggcggatt
     8341 gatgccgtgg ggctggcact gctggttatt ggtatcggca gcctgcagat tatgctcgac
     8401 cgcggtaaag agctggactg tttttcatca caggaaatta tcattcttac cgtggtggcg
     8461 gtggtggcta tctgcttcct gattgtctgg gagctgaccg acgataaccc gatagtcgat
     8521 ctgtcgttgt ttaagtcgcg caacttcacc atcggctgct tgtgtatcag cctcgcgtat
     8581 atgctctact tcggcgctat tgttctgctg ccgcagttgt tgcaggaggt ctacggttac
     8641 acggcgacct gggcaggttt ggcctctgcg ccggtaggga ttattccggt gattctgtcg
     8701 ccgattatcg gccgcttcgc gcataaactg gatatgcggc ggctggtaac cttcagcttt
     8761 attatgtatg ccgtctgctt ctactggcgt gcctatacct ttgaaccagg tatggatttt
     8821 tgcgcgtcgg cctggccgca gtttatccag gggtttgcgg tggtctgctt ctttatgccg
     8881 ctgaccacca ttacgctgtc tggtttgcca ccggaacgac tggcggcggc atcgagcctc
     8941 tctaacttta cgcgaacgct ggcggggtct atcggcacgt cgataaccac gaccatgtgg
     9001 accaaccgcg agtcgatgca ccatgcgcag ttgactgagt cggtaaaccc gttcaacccg
     9061 aatgcccagg cgatgtacag tcaactggaa gggcttggga tgacgcaaca gcaggcatca
     9121 ggctggattg cccagcagat caccaatcag gggctgatta tttccgccaa tgagatcttc
     9181 tggatgtcag ccgggatatt cctcgtactg ctggggctgg tgtggtttgc taaaccgcca
     9241 tttggcgcag gtggcggcgg aggcggtgcg cactaagtac aactaagcca gttcatttga
     9301 actggctttt ttcaattaat tgtgaagata gtttactgac tagatgtgca gttcctgcaa
     9361 cttctctttc ggcagtgcca gttcttcgtt gctgttgatg cgtacctcac gttccagaat
     9421 gctacgcgca atatcctgcg cttcctgcaa cgagtgcatc tggtaagtgc cacactggta
     9481 gacgttcagt tccgggatct gattctgatc ctgcactttc agcacgtctt ccattgccgc
     9541 tttccaggca tcagcaacac gctgctcatc tggcgtacca atcagactca tataaaaacc
     9601 ggtgcggcag cccattggcg agatatcgat aatctctaca ccattaccgt taagatggtt
     9661 acgcataaaa ccagcaaaca ggtgctccag ggtatggatc cctctttctg gcatcacttc
     9721 tttgttcggc acgcagaagc gcagatcgaa cacggtgatt gcgtcgccat gcggggtgtt
     9781 cattgttttc gccacccgaa ctgcaggcgc ttccatccgg gtatgatcga ctgtgaagct
     9841 atctaacaac ggcatttagc cacctccggt aattttttta aaaattttct gaactctttc
     9901 ttcccaggcg agtctgagta tatgaaagac gcgcatttgt tatcatcatc cctgaattca
     9961 gagatgaaat tttggccact cccgagtggc ctttttcttt tctgtcaggc gtgtttttcc
    10021 agccacaccg caaacggttc ggtatcagcg gtttccattt cctgctgacg gcgttcagat
    10081 gcctcgcgct cggctacaaa atcctcttcg cgcagaattt ccagcggctc ttcacgcagc
    10141 agattacggt aggcttctgc aaacgctttg cctgttccgc caataccagt atcaatcata
    10201 gaccttaaga tacgggcaga gaaagtcaga tcgggattat cgaagcaggc aaccagttca
    10261 tcacacactt tctgatacgc ttcgccgccg ttaatgctat ccagcgtttg cgcgacgcgt
    10321 ttcagatcgc ggaacagatc tttacccacc tgcagtaacg ggaactgcgc ggtttcgcag
    10381 ccgataccca gcgtcaggcc cggtttgcga ccttcaagga tcacccggtt ccagttaacg
    10441 cgcgtacagg caagttcgct actgctcatt tccggtgcat cagccagcgc acaccagacc
    10501 ataaacaggt cgaggaatcg cacctgctgt tcatctacac caatcggcga gaacgggttg
    10561 atgtccagcg aacgcacttc aatatattca atgccgccac gtaacagcgc atcagaaggt
    10621 gactcgccgc tgcgggtaac gcgttttgga cgaatcggcg catacagttc gttttcaatc
    10681 tgcagcacgt tgctattgat ttgcagcctc ttaccgtctt tctcaatacc aatcttcgcg
    10741 tactcttccg atggcgtttt gattgcctgt ttaaggcccg ctacgtactc gtaaagatcg
    10801 ttgaaggtaa taccaagatt gctttgcgat ttattggtat agccgagatc gctcaaacga
    10861 agagaggtcg catacggcag gtaatacata ccgcactcgg ttttctcaaa cggcagcgac
    10921 gttggttttc cttgcaggaa agaagaacaa atcgccggag atgcaccaaa cagataagga
    10981 ataacccaac cgaaacgata gtaattgcgg ataacgcgga aatagcccgc agaaattttc
    11041 tctttggcat cagcgcccga gatatcaccg cacttcgctt gccagaatgc cattggcaaa
    11101 gagaaattgt agtgcacgcc ggaaatggtt tgcatcagcg cgccgtagcg atttttcagc
    11161 ccttcacgat acagcgtttt aaagcgtccg gtgttagaag tgccgtactg tgccagttcg
    11221 atgtcctgac cttctgcgat gtagcatggc atacttaacg gccacatccg ctcatcgccc
    11281 atattgcgcg ccgtataacg atgcagatcg cgcataaagg tcagcatatg ttcaatatca
    11341 ccatccactg gtgtaatgaa ttccagcaat gcttccgcaa aatcggtagt aatccatttg
    11401 tgcgtcagtg cggaacctaa tgcttcagga tgacctgttg ttgccagtgt gccatcagca
    11461 ttaacacgca aagtttcgcg ctccagccca cgctgtatcc cctttaacgc ctgaggatgt
    11521 ttttccagcc aggccagcgc ctgtgatacg tccgggatca aattgacctc ccgcctgtca
    11581 aaatcgtttt aattagcata actgtaatgg tgaccatatg tgcaggccta caattagtgc
    11641 caccacatca tgccctgaac ggtcgctgct gcaactgcaa catagcgtaa cgctttacca
    11701 aggcacaaaa aaaagattac tggcccccag gagatgcgca tccatcccgc taacagacac
    11761 agtaaatcgc caaccacggg catccagctt aataatagcg tgactgcacc atagcgtttc
    11821 agccagccgg tagctttctc ttgccagcgc gatgttttac gcaatggaaa gaaacgccca
    11881 aggataacgt tagttaaccc tccaaggcta ttacccattg ttgctgttaa aactaaaacc
    11941 cagggatgac tgatcccgga aagcaacatt gccaccagca cgacttcgga gttgccgggt
    12001 aatagtgtag cgctgagaaa actactggca aacaacgaga aaagcgataa cgcttcactc
    12061 acagcaagcg aacatccaca gcgtccatgc ctgctgcacg ggccgcctga ataccgaaat
    12121 cggcatcttc aaagaccaca cactgcgtcg gttgcacgcc catacgctgc gcgcacaaca
    12181 aaaatgtgtc tggcgcgggt ttatggtgtt tgacgtgatc agcagcgacg acggcgtcaa
    12241 aataacggcg taatcccagg tgcgccagca atgcctcagc gatggcgctt tcactccccg
    12301 ttcctacagc cattgggcga cgaccatgcc aacttttcac cacttcaaca agaggaagcg
    12361 gctcgacact atccagcagc atacttctta ctgcttctgt tttttcacgc gctaacgcat
    12421 gcgggtcgag atcggcctga ttcagctcaa taattgcctg agcaatacgc caggtgggcg
    12481 atccattaag cgcaatcatc gcctgaatat cgtactgaag accgtagtgc cctaatactt
    12541 cgcgccacgc tttacggtgc gtaggcgccg tatccaggat tgtgccatcc atatcaaaaa
    12601 ttaaacctgc ataacgctcg tacatggtct tctcgcaagt cgaaaaatat gacgctactt
    12661 tagcgtaatt gcttgatttt gtcgctgatg gcgaaggaga agaaaattca ggaaatggat
    12721 aaagtagtta tttagaaatg agatattttt gaaggaaatt ttttggagaa tatggtgcat
    12781 ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat ccagctg
//
LOCUS       DAZPEU010000108        12379 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPEU010000108 DAZPEU010000000
VERSION     DAZPEU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.109"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="ACHU2L_002701"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="ACHU2L_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR1357494.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="ACHU2L_002702"
     CDS             complement(2005..2928)
                     /locus_tag="ACHU2L_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1357495.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="ACHU2L_002703"
     CDS             3145..4488
                     /locus_tag="ACHU2L_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1357496.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="ACHU2L_002704"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="ACHU2L_002704"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR1357497.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="ACHU2L_002705"
     CDS             6508..6732
                     /locus_tag="ACHU2L_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR1357498.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="ACHU2L_002706"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="ACHU2L_002706"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR1357499.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHU2L_002707"
     CDS             complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHU2L_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR1357500.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="ACHU2L_002708"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="ACHU2L_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR1357501.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="ACHU2L_002709"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="ACHU2L_002709"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR1357502.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLTRKK"
     gene            10315..10971
                     /locus_tag="ACHU2L_002710"
     CDS             10315..10971
                     /locus_tag="ACHU2L_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR1357503.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACHU2L_002711"
                     /pseudo
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACHU2L_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11503..12379
                     /locus_tag="ACHU2L_002712"
                     /pseudo
     CDS             11503..12379
                     /locus_tag="ACHU2L_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         3227 a         2902 c         3107 g         3143 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctgaca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac
    11281 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca taaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa
    12121 aaccagaaga cgctggcgag acttcagcga cagttaagcc gcaaggtcaa attcagcaac
    12181 aactggcaga agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc
    12241 agggactacc ttcacaaagt cacaacggcc gtcagcaaaa accacgcaat gatagtcatt
    12301 gaggatttga aggtcagcaa catgtcaaag tcagcagcgg gtacggtcag ccagccgggg
    12361 cgcaatgtcc gggcaaaat
//
LOCUS       DAZPEU010000109        12361 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPEU010000109 DAZPEU010000000
VERSION     DAZPEU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.110"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            759..842
                     /gene="dinQ"
                     /locus_tag="ACHU2L_002713"
     CDS             759..842
                     /gene="dinQ"
                     /locus_tag="ACHU2L_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1357504.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(896..2248)
                     /gene="gorA"
                     /locus_tag="ACHU2L_002714"
     CDS             complement(896..2248)
                     /gene="gorA"
                     /locus_tag="ACHU2L_002714"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR1357505.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2320..3162)
                     /gene="rlmJ"
                     /locus_tag="ACHU2L_002715"
     CDS             complement(2320..3162)
                     /gene="rlmJ"
                     /locus_tag="ACHU2L_002715"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR1357506.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3365..5407
                     /gene="prlC"
                     /locus_tag="ACHU2L_002716"
     CDS             3365..5407
                     /gene="prlC"
                     /locus_tag="ACHU2L_002716"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR1357507.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5415..6167
                     /gene="rsmJ"
                     /locus_tag="ACHU2L_002717"
     CDS             5415..6167
                     /gene="rsmJ"
                     /locus_tag="ACHU2L_002717"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR1357508.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6216..7684)
                     /gene="dtpB"
                     /locus_tag="ACHU2L_002718"
                     /pseudo
     CDS             complement(6216..7684)
                     /gene="dtpB"
                     /locus_tag="ACHU2L_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7850..7903
                     /locus_tag="ACHU2L_002719"
     CDS             7850..7903
                     /locus_tag="ACHU2L_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357509.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACHU2L_002720"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACHU2L_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR1357510.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="ACHU2L_002721"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="ACHU2L_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR1357511.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACHU2L_002722"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACHU2L_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR1357512.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="ACHU2L_002723"
     CDS             10967..12166
                     /locus_tag="ACHU2L_002723"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR1357513.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3035 a         3130 c         3310 g         2886 t
ORIGIN      
        1 gtaacagaaa tgacatattg cgctcctgat tgttgcaggt agtgtctctc ttcgaagcgg
       61 ataagtcaaa aacatatatg acttaacgaa tgtgtaagtg cagaggaagg taataggtgt
      121 gaattttgag ttggctattc atttgaaagg cggtttgaat caggacgtaa aaaaaccgac
      181 tttgcgtcgg ttttttactc ttcagcctga gttggtggct ctggctggag tgagaatgcc
      241 cacatctaac ggttcaatac tattagccag tggctaacat tgcaagcgtt agcgatttct
      301 tcagctggag aagggacagt ttgctataca tggtggtgaa agaggattta tatactttca
      361 tcgggtcgct gccgtaccaa gccttgtccg aatccggacg taaaaaaacc gacttcgcgt
      421 cggttttttt actcttcagc ccagagttgg tggctctggc tggagtgaga atgccatcat
      481 ctaacgcatc aatgctaata ccatactgaa actattgcaa ggttatgctg gttatttagc
      541 ctaattacac tgtatgatta tccagttagc tctgaggcat tttcactctg gcaatgcgca
      601 taaacgcttt caaagtcctg gtcagaagta cgggtggtgc cgttaactga tgctctggcc
      661 ggagtgagat tgcccacatc taacaatgag gcatgcgccg tgacaggcag tggatgagta
      721 agcggatgca ttctcactcc atcgcatgga gaaaacgggt gattgataaa gcaatcatcg
      781 ttcttggggc gttaattgcg ctgctggaac tgatccgctt tctgcttcag cttctgaact
      841 gatagcggaa acgtaattaa gggctaagag cacactactc ttagcccttt aacatttaac
      901 gcatggtcac gaactcttct gccgccgttg ggtgaatggc gacggtattg tcgaagtctt
      961 ttttggttgc gcccatcttc agcgccaccg cgaagccctg caacatttcg tccataccaa
     1021 agccaatgcc gtgaataccg acaatcttct cttctggccc aacgcacacc agcttcatgc
     1081 ggcacggctg gcggtgagtg gtgacggcgg tatacatcgc ggtgaaagag gatttataca
     1141 ctttcacctg atcgtcgccg tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtac
     1201 caatcggcgg atggctgaag accacggtcg gaatgttgct gtaatccaga tgctcatccg
     1261 gcttgttatt aaacaggcgt tcagagagac ggcgacccgc tgcaactgcc accggtgtca
     1321 gttccactgc acccgtgtta tcgcccaccg cgtaaatacc ttcaatattg gtgttttgat
     1381 atttatcgac gacgatatag cctttttcgt tggttttaac gccagcggct tcgaggttga
     1441 tgttgtcatt ggcaggctcg cgaccaatcg cccaaatcag gcaatccacc gtttcactgc
     1501 gaccatcttc cagctccagc gtcaggctac catcggcatt tttcactacc gctttcggga
     1561 tggcgttggt atgcaactgt gggccttcgg cgttcatcac ttcgaccagc gtttcagaga
     1621 tcatcgggtc gaagctgcgc agcggcgcat gtttacgcac aaacagatgc gttttcgcgc
     1681 cgaggccgtt aatcacgcca gccagctcaa cggcgatgta acccgcgcca acaaccgcca
     1741 cgcgctctgg caaagcagga agggcgaaga agccatcgga atcaataccg tattccacgc
     1801 ctggaatatc cgggtggctc ggacgaccgc cggtggcgat cagaatatga tcggccgtga
     1861 tggtttcgcc gttaacttcc agcgttttgg catcaacgaa gcgggcaaag cctttgatta
     1921 catcaacgtt atttttaccg agcacgtttt cataggaagt atgaatacgg tcgatatagg
     1981 cggtacggct ggcgatcaac gtttcccagt tgaacttgtt gatggtggta tcgaaaccat
     2041 aatcagggcc gtacatatgg atcgcttcac ggatttgcgc cgcgtgccac atcacttttt
     2101 tcggcacgca gccgacgttc acgcaggtgc cgcccagctc tttggcttca atcagtgcgc
     2161 atttctggcc gtacatagcc gcgcggttga tggaggcgat accgccgctg ccgccgccga
     2221 tggcgatgta atcatagtgt ttagtcatga catattgtcc ttatcgttga ttaccgcgat
     2281 tgtagcgcga gacgtaatag gtgccagcaa tggctgcaat tactccggca cgatccagct
     2341 tacggtggcg tgcccggtac ctgccggaac cagtttgctg tgcagccacg acagcacgtt
     2401 attcatctgc tgttccagct tccacggcgg gttaatcaca atcatgccgg aagcggtcat
     2461 gccacggcga tcgctgtctg gcagcaccgc cagttcaatt tgcagaattt tgcgaatgcc
     2521 ggtcgcttcc aggtcgtgga tcatgcgctt aatttgctgg cgcagcacca ccggatacca
     2581 caatgcgtaa gtgccagtgg cgaaacgttt gtaaccttct gctatcccgc tgaccaccgc
     2641 ctgatagtcg gttttcattt catacggcgg gtcgataagg attaaaccac ggcgagaaac
     2701 cggcggcagt ttggccttaa gttgctggaa accgtcggct ttttcgacgc gtgcacggct
     2761 gtctttctga aatccagaac gcagcaacgg gtaatcgctc gggtgcagtt cggtcagttg
     2821 caggctgtct tgttcgcgca gtagctggcg agcaatcaac ggcgaaccgg ggtagtaacg
     2881 caactggccg ctacggttga agtgttttac cacattgatg tacgcctcca gttctgcggg
     2941 caaatcgtcc tgctgccaga tacgggcgat accttcgaga tattcgccgg tacgctcggc
     3001 atgttcgcta cctaactgat aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg
     3061 tttatctttc tctttcagcg actcgatgat caggctctga acggtatgtt taaggacgtc
     3121 ggcgtggttg ccagcgtgaa agctgtggcg ataactgagc atgggtaaag gtgttccggt
     3181 aagtaaaaat tggcccgtat tcggggcgca caaaagccta tcaggacagt ataccgaaaa
     3241 gaggccgccg ccgcgaaagc gtaacgtttc tcattgaaat tcactacact taaccccatg
     3301 ctacacacat tatgtaaagc gcctgttgag cgcttcctta acctctttaa ccaggactgc
     3361 gctaatgacg aatccgttac tgactccctt tgaattgcct ccgttttcta aaattctccc
     3421 ggaacatgtc gttccagccg tgactaaggc attgaacgac tgccgcgaaa atgtggagcg
     3481 cgtagtagcg caaggggcac cgtacacctg ggaaaatctc tgccagccgt tggcggaagt
     3541 ggacgatgtt ttggggcgta tcttctcccc ggtcagccac ctgaactcgg tgaaaaatag
     3601 cccggaactg cgtgaagcgt acgaacaaac cctgccgctg ctgtcggaat acagcacctg
     3661 ggtagggcaa catgaagggc tgtataaagc gtaccgcgac ctgcgcgatg gcgatcatta
     3721 cgccacgctg aacacggcgc agaagaaagc ggttgataac gccctgcgtg attttgaact
     3781 ctctggcata ggtctgccga aagagaaaca gcagcgttac ggcgaaattg ctacccgtct
     3841 ttctgaactg ggcaaccagt acagcaacaa cgtcctcgat gcgacgatgg gctggaccaa
     3901 actcgttacc gacgaagcgg ggctggcggg gatgccagaa agcgcgctgg ctgcggcaaa
     3961 agcccaggcc gaagcgaaag agctggaagg ctacctgctg acgctggata tcccaagcta
     4021 cctgccggta atgacctact gcgacaacca ggctctgcgc gaagagatgt atcgcgctta
     4081 cagcacccgc gcctccgatc aaggcccgaa tgccggtaag tgggataaca gcaaggtgat
     4141 ggaagagatc ctcgcgctgc gtcacgaact ggcgcaactg ctgggctttg aaaactacgc
     4201 ctttaaatcc cttgccacta aaatggcaga aaacccgcag caggtgctgg atttcttaac
     4261 cgatctggca aaacgcgcgc gtccgcaagg cgaaaaagag ctggagcaac tgcgtgcctt
     4321 cgctaaagcc gaatttggtg tcgatgagtt gcagccgtgg gatatcgctt actacagcga
     4381 aaaacagaaa cagcacctct acagcatcag cgacgagcag ctgcgtccgt acttcccgga
     4441 aaacaaagcg gttaacggcc tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa
     4501 agagcgtaaa gatgttgatg tctggcatcc ggatgtacgt ttcttcgaac tgtatgacga
     4561 aaataacgaa ctgcgcggca gcttctacct cgatctgtat gcccgtgaaa acaagcgcgg
     4621 cggggcgtgg atggatggct gcgtaggcca gatgcgtaaa gctgatggtt ctctgcaaaa
     4681 accggtcgcg tatttgactt gtaacttcaa ccgcccggta aatggtaaac cggcgctgtt
     4741 cactcacgac gaagtgatca ccctgttcca cgagttcggt cacggcctgc accatatgct
     4801 gacccgcatc gaaaccgctg gtgtttccgg tatcagcggt gtgccgtggg atgcggtcga
     4861 actgccgagt cagtttatgg aaaactggtg ctgggagccg gaggcgctgg cgtttatctc
     4921 tggtcactat gaaaccggcg aaccgctgcc gaaagagttg ctggataaaa tgctggcggc
     4981 gaagaactac caggcggcgc tgtttattct gcgtcagctg gagttcggcc tgtttgattt
     5041 ccgccttcat gccgagttcc gcccggatca gggggcaaaa atcctcgaaa ctctggcaga
     5101 aatcaagaaa ctggttgccg tggtgccatc tccgtcctgg ggccgtttcc cgcacgcttt
     5161 cagccatatt ttcgccggtg gttatgcggc aggttactac agctacctgt gggctgacgt
     5221 actggcggca gatgctttct cgcgctttga ggaagagggc attttcaacc gtgaaaccgg
     5281 gcagtcgttc ctcgacaaca ttctgagccg tggcggttca gaagagccga tggatctgtt
     5341 caaacgcttc cgtggtcgtg aaccgcagct ggatgcgatg ctggagcatt acggcattaa
     5401 gggctgatca ttcagtgaaa atctgcttaa ttgatgaaac aggcgccgga gacggcgcct
     5461 tatctgttct ggcggcccgc tgggggctgg agcacgatga agacaacctg atggcgctgg
     5521 tgttaacgcc ggaacatctg gaattgcgca agcgtgatga gccaaaactt ggcggcatct
     5581 ttgttgattt tgttggcgga gcgatggcgc accgacgcaa attcggcggt ggtcgcggtg
     5641 aggcggtggc gaaagcggtg ggcattaaag gcgattattt gccggatgtg gtggacgcca
     5701 ccgcagggct ggggcgcgat gcctttgtac tggcttcagt cggctgccgc gtgcggatgc
     5761 tggagcgtaa tccagtggtt gccgcgctac tcgacgacgg cctggcgcgt ggttatgcgg
     5821 atgcggaaat cggcggctgg ttgcaggagc ggttgcagtt aattcacgcc tccagcctga
     5881 cggcgctaac cgatattacc ccacgcccgc aggtggtcta tctcgatccg atgttcccgc
     5941 ataagcagaa aagcgcgctg gtgaaaaaag agatgcgtgt ctttcagtcg ctggtggggc
     6001 cggatcttga tgccgatgga ttactggagc ctgcacgctt gctggcgacc aaacgcgtgg
     6061 tggtaaagcg cccggactac gcgccaccgc tggcgaatgt cgccacgcca aacgcggttg
     6121 tgactaaagg gcatcgcttt gatatctatg caggtacgcc agtgtaaaag taaagcaggg
     6181 tagcgataac gctaccctgc tttctgcaag aataattaat ggctttccgg cgtcgcaatc
     6241 atgcgtttca gccacggcac catcaacagc atcactactg caacgcccag cgtgaccaga
     6301 ccaatcttac caaacacgtt ggtatagacg ggcaacgtct caagcggatc ggtaatgttg
     6361 tccggcaccg cggtaaatgt tgccacatag ccgccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggtgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 g
//
LOCUS       DAZPEU010000110        12152 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPEU010000110 DAZPEU010000000
VERSION     DAZPEU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.111"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            126..995
                     /locus_tag="ACHU2L_002724"
     CDS             126..995
                     /locus_tag="ACHU2L_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1357514.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACHU2L_002725"
     CDS             complement(1198..1551)
                     /locus_tag="ACHU2L_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1357515.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHU2L_002726"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHU2L_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1357516.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACHU2L_002727"
     CDS             complement(3379..3672)
                     /locus_tag="ACHU2L_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1357517.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHU2L_002728"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHU2L_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1357518.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACHU2L_002729"
     CDS             complement(5220..5696)
                     /locus_tag="ACHU2L_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1357519.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACHU2L_002730"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACHU2L_002730"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1357520.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHU2L_002731"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHU2L_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1357521.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACHU2L_002732"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACHU2L_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1357522.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHU2L_002733"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHU2L_002733"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1357523.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACHU2L_002734"
     CDS             11052..11627
                     /locus_tag="ACHU2L_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1357524.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACHU2L_002735"
     tRNA            11734..11809
                     /locus_tag="ACHU2L_002735"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2931 a         3140 c         2980 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa ca
//
LOCUS       DAZPEU010000111        12114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPEU010000111 DAZPEU010000000
VERSION     DAZPEU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.112"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..371
                     /locus_tag="ACHU2L_002736"
     CDS             <1..371
                     /locus_tag="ACHU2L_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357525.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACHU2L_002737"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACHU2L_002737"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR1357526.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACHU2L_002738"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACHU2L_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR1357527.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACHU2L_002739"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACHU2L_002739"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR1357528.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACHU2L_002740"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACHU2L_002740"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR1357529.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACHU2L_002741"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACHU2L_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR1357530.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACHU2L_002742"
     CDS             complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACHU2L_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR1357531.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACHU2L_002743"
     CDS             complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACHU2L_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR1357532.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACHU2L_002744"
     CDS             complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACHU2L_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR1357533.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACHU2L_002745"
     CDS             complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACHU2L_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR1357534.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACHU2L_002746"
     CDS             complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACHU2L_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR1357535.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACHU2L_002747"
     CDS             complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACHU2L_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR1357536.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11913..>12114)
                     /locus_tag="ACHU2L_002748"
     CDS             complement(11913..>12114)
                     /locus_tag="ACHU2L_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR1357537.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2785 a         3388 c         2933 g         3008 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgccag ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg
     6361 ctgtgtaata gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta
     6421 acggtgccat gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg
     6481 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
     6541 gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
     6601 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
     6661 gggaagaaaa aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
     6721 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
     6781 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
     6841 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
     6901 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
     6961 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
     7021 ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa
     7081 gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
     7141 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
     7201 caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
     7261 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
     7321 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
     7381 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
     7441 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
     7501 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
     7561 aaggttttta accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg
     7621 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
     7681 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
     7741 ccgctcgccg ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac
     7801 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
     7861 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
     7921 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
     7981 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc
     8041 aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
     8101 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
     8161 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
     8221 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
     8281 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
     8341 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
     8401 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
     8461 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
     8521 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
     8581 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
     8641 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
     8701 tggcgaaact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
     8761 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
     8821 tttcaccacg tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
     8881 gccggccttc aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc
     8941 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
     9001 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgtagctgg cggcatccgg
     9061 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atatccttcg tttccaaaaa
     9121 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
     9181 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
     9241 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
     9301 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
     9361 ctgataaaaa tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg
     9421 tgggaacact gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct
     9481 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc
     9541 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
     9601 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
     9661 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
     9721 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag
     9781 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
     9841 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
     9901 ttttgagatg gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
     9961 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    10021 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    10081 tttcactatt ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt
    10141 actccgcaat ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa
    10201 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    10261 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    10321 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    10381 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    10441 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    10501 gctggtttcg gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc
    10561 caataaaata ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    10621 ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc
    10681 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    10741 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    10801 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    10861 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    10921 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    10981 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    11041 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    11101 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    11161 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    11221 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa
    11281 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    11341 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    11401 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    11461 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    11521 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    11581 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    11641 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    11701 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    11761 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    11821 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    11881 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    11941 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    12001 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    12061 cggaagtgac aaaccagctg attggtttct gccagcttaa ccatttgcgg aaga
//
LOCUS       DAZPEU010000112        12074 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPEU010000112 DAZPEU010000000
VERSION     DAZPEU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.113"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(16..392)
                     /gene="rnpB"
                     /locus_tag="ACHU2L_002749"
     ncRNA           complement(16..392)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHU2L_002749"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(425..1570)
                     /gene="garK"
                     /locus_tag="ACHU2L_002750"
     CDS             complement(425..1570)
                     /gene="garK"
                     /locus_tag="ACHU2L_002750"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR1357538.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1667..2557)
                     /gene="garR"
                     /locus_tag="ACHU2L_002751"
     CDS             complement(1667..2557)
                     /gene="garR"
                     /locus_tag="ACHU2L_002751"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR1357539.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2587..3357)
                     /gene="garL"
                     /locus_tag="ACHU2L_002752"
                     /pseudo
     CDS             complement(2587..3357)
                     /gene="garL"
                     /locus_tag="ACHU2L_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3373..4707)
                     /gene="garP"
                     /locus_tag="ACHU2L_002753"
     CDS             complement(3373..4707)
                     /gene="garP"
                     /locus_tag="ACHU2L_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR1357540.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5082..6653
                     /gene="garD"
                     /locus_tag="ACHU2L_002754"
     CDS             5082..6653
                     /gene="garD"
                     /locus_tag="ACHU2L_002754"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR1357541.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6705..7529)
                     /gene="agaR"
                     /locus_tag="ACHU2L_002755"
     CDS             complement(6705..7529)
                     /gene="agaR"
                     /locus_tag="ACHU2L_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR1357542.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7778..9058
                     /gene="kbaZ"
                     /locus_tag="ACHU2L_002756"
     CDS             7778..9058
                     /gene="kbaZ"
                     /locus_tag="ACHU2L_002756"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR1357543.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9081..9554
                     /gene="agaV"
                     /locus_tag="ACHU2L_002757"
     CDS             9081..9554
                     /gene="agaV"
                     /locus_tag="ACHU2L_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR1357544.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9565..10344
                     /gene="agaW"
                     /locus_tag="ACHU2L_002758"
     CDS             9565..10344
                     /gene="agaW"
                     /locus_tag="ACHU2L_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR1357545.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10334..11143
                     /gene="agaE"
                     /locus_tag="ACHU2L_002759"
     CDS             10334..11143
                     /gene="agaE"
                     /locus_tag="ACHU2L_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR1357546.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11230..11664
                     /gene="agaF"
                     /locus_tag="ACHU2L_002760"
     CDS             11230..11664
                     /gene="agaF"
                     /locus_tag="ACHU2L_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR1357547.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11661..>12074
                     /locus_tag="ACHU2L_002761"
     CDS             11661..>12074
                     /locus_tag="ACHU2L_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1357548.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2882 a         3253 c         3079 g         2860 t
ORIGIN      
        1 gtttttacgt aaaacaggtg aaactgaccg ataagccggg ttctgtcgtg gacagtcatt
       61 catctaggcc agcaatcgct cactggctca agcagcctac ccgggttcag tacgggccgt
      121 accttatgaa cccctatttg gccttgctcc gggtggagtt taccgtgcca cggactgtta
      181 ccagccgcgc ggtgcgctct taccgcaccc tttcaccctt acctgatccc gcttgcacgg
      241 gccatcggcg gtttgctctc tgttgcactg gtcgtgggtt tcccccccag gcgttacctg
      301 gcaccctgcc ctatggagcc cggactttcc tcccctccac ccgtctcccc cgaagaggac
      361 gacgacgaag cggcgactgt ctggtcagct tcggcgcgca gtatagaggg tttgcgcgcc
      421 cttgtcaccc cgtgttgcgc attccaatcg ccagtgtcgc ggcaatatta cgtgaagcac
      481 ggcagatatt gtcataagcc ccgcggaatg cttcgtccaa cgtacctatg ctggtcaata
      541 cgctgaagac cgcatcaatg ccatgctgat gtacaacgcc aacatcatcg gtcaggctac
      601 ccgcaatgcc aatcaccggt ttatggtact tcttcgccac gtttgcgacg ccaatcggta
      661 ccttgccgtg aatactctgg ctgtcaatac gcccttcacc ggtgatcacc agcgtacaat
      721 cgtgaatatg ttcctccaga ttcagcgccg tagtgacgat ttcaatacca cttttcagtt
      781 ccgcaccaag aaacgccatc agcgccgcac ccataccgcc cgcagctcct gcgccgggga
      841 catcttttac atcaacatgc agcgcttttt taatgacatc ggcatagtga gagaggttat
      901 tgtccagctc aacaatcatc gcttcactgg ctcccttttg tgggccaaag atgcgcgatg
      961 cgccgttatc gcccaccagc ggattggtga catcacaagc gacgcgaatg acgcaatctt
     1021 ttaagcgcgg atcgaggccg gaaatatcaa tatcattcag agtattaaga ctaccgccgc
     1081 caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc ccccagcgcc tgtaccatgc
     1141 ctgcgccgcc atcatttgta gcgctaccgc caatgccgat aataatgttt gtcgcgccgc
     1201 tctccagcgc ctgcaggatt aactcgcctg tgccgcgtga agtggtcacg agtggatcgc
     1261 gtttttccgc aggtaccagc tccagcccac tggccgccgc catttcaata aacgcggttt
     1321 tgccatcgcc ggagatcccc caactggcat tcactttctc gcccagcggc cctgtaaccc
     1381 aggcgtgacg ttcagaaccc tgggtggctg caatcatcgc ttccaccgtt ccttcgccac
     1441 cgtcggcaac cggaatagaa acgtactgtg catcaggaaa aatttcccga aatccttttt
     1501 ctatcgcctg cgcaacctcg ctggcagata aactttcttt ataagagtct ggggcgatta
     1561 cgattttcat acctatgcct gttaccacat gacgccggag ggcgtttctc ttattcggcc
     1621 tggattccag gcccggattg caatacgcca tccgggcacg acgtcattaa cgagtaactt
     1681 cgactttcgc cagtttttcg tagtagcaca ccagggcgct atgatccgcc gttcctaaac
     1741 catctgctcg cagtgcctgc atcatctcca taaccgcagc tgtgagcggc agttgcgcgc
     1801 cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt aatatgcaga tcaatacgga
     1861 agcccggctt gaagttgcgg tccatcacca tcggcgcttt ggcatccagc acggtactgc
     1921 ccgccagtcc accgcgaatt gcctgataca ccaggtccgg gttaacgccc gctttagttg
     1981 ccagcgttaa cgcttctgac atcgcggcaa tattcagcgc cacaatgacc tgattcgcca
     2041 gtttggtgac gttacctgca ccaattgccc cggtatgcac cacggaaccc gccattgctt
     2101 tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc gcccaccatc actgacagcg
     2161 taccgtcgat ggctttcggt tcaccgccgc tcaccggagc atccagcata tcaatgcctt
     2221 tcgctttcag cgcttcgctg atttcacggc ttgccagcgg tgcgatagaa ctcatatcga
     2281 tcaataccgt acctggcttc gcgccttcaa taatgccatt ctcacccagc gccacctctt
     2341 tcacatgagg ggagtttggc agcatggtta tgatgacgtc gcactgttca gcgatcgctt
     2401 tagccgtaga ggctgtttct gcacctgcag caatcacgtc agcaatagct tctgggttac
     2461 ggtcagcaac caccagcgag taacctgctt tcagaaggtt tttactcatt ggtttaccca
     2521 taatccccag gccaataaaa ccaactttca tagtcatatc aatcatctct cttgttgcgg
     2581 tggtggttat tttttaaagg tatcagccag tttatgagtg gcagagcgga agacgccgag
     2641 atcgctgccg acagccacaa acgtcgcgcc ccattccaga taacgacgcg catcggcttc
     2701 gaccggcgcg aggataccgc tgggtttgcc gtgcgcgctg gcacggttaa aaatgtgctg
     2761 aattgctttt tgtacatccg ggtgtgatgc attgccgaga tggcctaatg ccgcggccag
     2821 atcgctgggg ccgacgaaga tgccgtctac gccttcggta gcggcaatgg catcgacgtt
     2881 atctacgccc tgctgacttt ctatctggac cagaatagtg atgttcttgt tcgactgagc
     2941 gaaataatcc gccacggtgc caaacatatt ggcgcggtga gaaacggaga cgccgcgaat
     3001 gccttccggt gggtaacggg ttgatgccac cgcctgctct gcttcctctt ttgtttctac
     3061 aaaaggaatc aggaagttat agaaaccgat atccagaaga cgcttaataa tttccggctc
     3121 gttggtcggc actcacacta ctggcgcgct ggcgctgcct ttcaaggcca ttaactgcgg
     3181 aataaacgtg gagatatcgt ttggcgcatg ttcgccatcc agcaccagcc agtcaaaccc
     3241 agccaaacca agaacttcag tgctaatcgg gttagagagt gctgaccagc aaccaatttg
     3301 tacctgtttc gcagccagtg cggctttgaa tttattcggg aaaacatcgt tattcatcgc
     3361 ttataccttt gcttatttct gcaattccat acgtttaatg tcgccaacta cgaagaggta
     3421 gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc agtgctgcat tgaaggagtg
     3481 cagttcactt accaggtagc caatcaccag tggagtgaca atggaggcaa cattgccaaa
     3541 gacgttaaag acgccgccac agaggccaac aatctctttc ggcgcggtgt cagaaatcac
     3601 cggccagccc agagcaccaa atcctttgcc aaaaaaagcc agcgccatca gcatgaccac
     3661 cagcgtggtg ttgttggtgt agttacataa gatgatggtg gaagccagca acattcccag
     3721 cacaatcggt agcttacgtg ccagggtcag ggataaaccg cgtttgatca gataatccga
     3781 gaagacacct cccagcacgc cgcccgcaaa accacacagt gccggaatcg aggcgaccag
     3841 acccactttc agaatcgaca tgcctttttc ctgcaccaga taaatcggga accaggtgag
     3901 gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa aatacgccca acatcatgcg
     3961 gttagagagc aattgcttga tgtaatgcag tttgggaccg cttgctgccg cgctgcccgg
     4021 ctttttgtgg tccatatcga ccaccgcgcc attttcagag ataaacttca gctcttcctc
     4081 agacatacgt gggtgatctg tcgggttatg aatcaacttg atccacagcg ccgtcagcac
     4141 aaaacaaatc acccccataa cggtaaagac gtgctcccat ccccaggcga aagtcagcca
     4201 gccaagcaga ggcgaaaaga gcgccagcga gaaatattgt gccgagttaa agatggcgga
     4261 ggcagtacca cgttctttcg tcgggaacca ggcggcgaca atacgggcgt tcgccgggaa
     4321 ggatggagct tccgagaatc ccagcataaa gcgcataaag aacatggaga tccctgccca
     4381 agccagcggg aacatatcaa caaagccttg caggaaggtg aacagcgacc agaaaaagag
     4441 gctgtaggtg taaacttttt tcgagccaaa cttatcaagc aaccagccgc cggggatttg
     4501 catcagcaag taggcccagc caaaagcgga gaagatgtaa cccatcgaaa ccgcacttaa
     4561 ctgcaactct tttgccactt cggtaccagc aatagacagc gttgcacgat cggcgtagtt
     4621 aacggcggta acaataaaaa taatcagtaa tattaaatag cgggtatgca cacctttcct
     4681 tttttcgtca acggtgtcca gaatcatttt atttacctcg ggtacttatg ctgattttta
     4741 ttattatggg gaaggtgtta tttataagtt tcatttatgc cgtaacgaca atgaactcgg
     4801 gaattagtat aagcagcgcg agaataataa tcattgtgca aatgctaatt taattaatac
     4861 tttttaaata ttattttgag catatgcaca taaggttgcg cgctaaagca cagatttgcg
     4921 ctttaccttg ccgggcggca ctgcaatccc taaaatgatt gacattgatc acatttctgc
     4981 gtttaaactc ctgacattct tatttcaccc aatgaaatca tttattttta aatgagacca
     5041 ggtcctcatt ttaataaccc ctggctggag aatattgcac aatggccaac atcgaaatca
     5101 gacaagaaac gccaactgcg ttttatataa aagttcacga cacagataat gtggcaatta
     5161 ttgttaatga taatggcctg aaagcaggaa cgcgttttcc ggatgggctg gaattaattg
     5221 aacatattcc ccaggggcat aaagtcgcat tgctggacat tccggctaat ggtgaaatta
     5281 ttcgttatgg cgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga agctggatcg
     5341 acgaatcaat ggttgtacta ccggaagcgc cgccgttaca cacgctgcca ctggcaacca
     5401 aagtcccgga acccttaccg ccgctggaag gatacacctt tgagggctat cgcaatgccg
     5461 atggcagcgt gggcaccaaa aacctgctcg gcatcaccac cagcgtccac tgtgtggcag
     5521 gcgtggtgga ctatgtagta aaaatcattg aacgcgatct gctaccgaaa tacccgaacg
     5581 tcgatggcgt ggtggggctg aatcatttgt acggttgtgg cgtggcgatt aacgcaccgg
     5641 cagcagttgt acctattcgt accattcaca atatttcgct gaaccctaac tttggcggcg
     5701 aagtaatggt gattggcctg ggttgtgaaa agttgcagcc tgagcgcctg ctgactggaa
     5761 cggatgatgt gcaagctatt ccagtagaaa gcgccagcat tgtcagtttg caggatgaaa
     5821 agcatgtcgg ttttcagtcc atggtcgagg atattttgca ggtcgccgaa cgccatctac
     5881 aaaaactgaa tcaacgtcag cgagaaacct gtccggcttc agaactggtt gtcggcatgc
     5941 attgcggtgg cagcgatgcg ttttctggcg taacggcaaa cccggcggtt ggctatgcgt
     6001 ctgatctact ggtgcgctgc ggcgcaacgg tgatgttttc agaagtcacg gaagtgcgtg
     6061 acgcgatcca tctgctgaca ccacgcgcag tgaacgaaga ggtcggcaaa cggctgctgg
     6121 aggagatgga gtggtacgat aactatctca atatgggaaa aaccgaccgc agcgccaacc
     6181 cttcgccggg caacaagaaa ggcggtctag caaacgtggt agagaaagcg ctcggctcca
     6241 ttgctaaatc gggcaaaagt gcaattgttg aagtgctgtc gcccggtcaa cgcccgacta
     6301 aacgcggatt aatttacgcc gcgacgccag ccagcgattt tgtctgtggc acgcaacagg
     6361 tggcttcggg tatcaccgtg caagtgttta cgaccggtcg tggtacgccg tacggcctga
     6421 tggcggtacc cgtcattaaa atggcacccc gcaccgagct ggcgaaccgc tggtttgatt
     6481 taatggatat taatgcgggc accatcgcta ccggcgaaga aactattgaa gaggagggct
     6541 ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc ttctcggatc
     6601 aatggggact gcataaccag ctggcggtgt ttaacccagc accggtgacc tgataattca
     6661 attcgtatta tgcccgatag cacacgttta tcgggcatat tgagtcacag cgaagacgcg
     6721 tgatccccga ccagaatcac ttcaacccca gcctttcgca gtccttccag actatccgca
     6781 ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat cttatgtaaa
     6841 ctggaacgat tgaatttact ggaatcggtg accacgatga tccgttccgc aacttcgcac
     6901 atccgacggc ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg
     6961 atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga ttgctcagcc
     7021 tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat cagcagctca
     7081 acgccttccg cttccagcaa cgcattagcc acgttcatac cgttggtcat cgcaattacg
     7141 tcagtgtact tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga atcgaggatc
     7201 acccgatgac ctggctgaat caactcaacg gcagctttcg caacgctgcg tttcatcgcg
     7261 gtgttcagtg cgcttttatc ttccactgat ggctcgactg acggcgtcgt gctatcgcag
     7321 atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa cgccagatca
     7381 ttgcggatcg tcacagtaga tacgccatac aatgccgaca gatcgttaac ctgcacactc
     7441 ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc tgtcactcgc
     7501 ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact ttcgtttcat
     7561 ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccacctt gcccattggc
     7621 gcaagctact ctcgtttcag tgactttcat tacgtttctt ttgtgaatca gatcagaaaa
     7681 ctattatctt tcgttttatt tttatctcac catgacgcag tatcaattga aacaaaacga
     7741 aagatcaata tcgcagtaat ctgaactgga gaggaaagtg aaacatctga cagaaatggt
     7801 gagacagcac aaagcgggca aaacaaatgc aatttatgcc gtttgttccg cacatccgct
     7861 ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa acgccgttac tgattgaagc
     7921 aacctccaat caggtagacc agttcggcgg ttataccgga atgacgcccg ccgattttcg
     7981 cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgc tgattctggg
     8041 tggtgaccat ctggggccaa accgctggca aaacctgcca gccgctcagg caatggccaa
     8101 tgccgatgat ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc accttgattg
     8161 cagcatgtcc tgtcaggacg atccaattcc cttaactgat gacatcgtgg ctgaacgcgc
     8221 cgcccgtctg gcgaaagtgg cggaagaaac ctgtcgcgaa cactttggcg aagccgatct
     8281 ggagtatgtc attggtaccg aagtgccggt acctggcggc gcgcatgaaa ccttaagcga
     8341 gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc gtcacgcctt
     8401 tgaaaagcaa ggtttgaatg ccatctggcc acgcattatt gccctggtgg ttcaacccgg
     8461 cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga gcgccttaag
     8521 ccagatggtc gaaaactacg aaacgctgat tttcgaagcg cactctaccg attatcaaac
     8581 gccgcaatct ctgcgccagc tggtgattga ccactttgcc attctgaaag ttggcccggc
     8641 gctgaccttc gccctgcgtg aagccctttt ttctctggcg gcgattgaag aagaactggt
     8701 accagcgaaa gcctgttccg gtctgcgtca ggtgctggaa aacgtgatgc tcgaccgccc
     8761 ggaatactgg caaagccact accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta
     8821 cagctactca gatcgcgtgc gctattactg gccggacagc cagattgatg acgctttcgc
     8881 tcatctggtt cgtaatctgg cggattcacc aattccgcta ccgctcatca gccagtatct
     8941 gccgctgcag tacgtgaaag ttcgctccgg cgagctgcaa ccgacgccac gggaactcat
     9001 tataaaccat attcaggaca tcctggcgca gtaccacaca gcctgtgaag gccaataagc
     9061 aaaacaaaga ggaacatgct atgccaaata ttgttttaag ccggattgat gaacgcttga
     9121 ttcacggtca ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg gtgctggtag
     9181 ccaacgatga ggttgccgaa gatccggtac aacaaaacct gatggaaatg gtactggcag
     9241 aagggatcgc cgtacgtttc tggacgctgc aaaaagttat cgacaacatt catcgcgccg
     9301 ccgatcgaca gaaaatcctg ctggtttgta aaacacccgc cgatttcctg acgctggtga
     9361 aaggtggcgt tccggtgaat cgcattaacg ttggcaatat gcactacgcc aatggcaaac
     9421 aacaaatcgc caaaacggtt tctgtggatg cgggcgatat cgcagcattt aacgacctga
     9481 aagccgctgg ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct gtggacctct
     9541 ttaaattact ttgagggatt catcatggaa atcagcctgt tgcaggcatt tgcgttgggc
     9601 attatcgcct ttatcgctgg cctagatatg tttaacggct taacccatat gcaccgcccg
     9661 gtggtcctcg gcccgttggt cgggctggta cttggcgatc tgcataccgg aattttaacc
     9721 ggcgatacgc tggaactagt gtggatgggg ctggccccgc tggcgggcgc acagccgcct
     9781 aacgtgatta tcggtactat cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc
     9841 gatgtcgcgg tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg gattaccttc
     9901 ctgttctcgg tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat
     9961 actcgcggca ttgaacgcgt gaactatctg gcactgctgg cactcggcac tttctatttt
    10021 ctctgcgctt tcctgcctat ctacttcggc gcggaacatg caaaaaccat cattgatgtc
    10081 ctgccgcaac gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt
    10141 tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca taccctactt catcctgggc
    10201 tttgttgccg caacctggct caagttaccg gtgctggcta ttgctgccgc cgcgctggcg
    10261 atggcgctga tcgatctgct gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa
    10321 gaggaatttg aagatggcat ctaatcaaac caccctgccg aacgtcagcg aaaatgaaga
    10381 aacactgctg actggcgtca atgaaaacgt gtatgaagat cggagcattg ccgcggagct
    10441 gacgaaaaaa gatatcaacc gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa
    10501 ctacgaacgt atgcaggctt ctggctggtt gtacggtctg ctgcctgctc tgaaaaagat
    10561 ccacactaat aaacgcgacc tggcgcgcgc catgaagggg catatgggtt tcttcaatac
    10621 ccatccgttt ctggtgacgt ttgttatcgg cattatcctt gcgatggagc gttctaagca
    10681 ggacgttaac agtattcaga gcaccaaaat tgccgtcggt gcgccgcttg gcgggattgg
    10741 cgatgcgatg ttctggctaa cgctactgcc gatttgtggc gggataggcg caagcctcgc
    10801 gctacaaggc tctattcttg gcgcagtcgt ctttattgtg ctgttcaacg tggtacactt
    10861 ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg cgattcccct
    10921 tattaaagcg aataccaaaa aagtcggcca tgctgcgtca atcgttggaa tgacggtaat
    10981 cggcgcactg gtggcaacct atgttcgttt aagcaccacg ctggaaatca cggcgggcga
    11041 cgcagtagtt aagttacagg ctgatgttat cgacaaactg atgcctgcct tcttaccgct
    11101 ggtctacacc ctgaccatgt tctggctggt acgccgcggc tagagtccgc tgcgcctgat
    11161 tgcggttacc gtggttctag ggatcgtcgg taaattctgc catttccttt aaatacaatg
    11221 aggtattaga tgttaagtat tattttgaca gggcatggcg ggtttgccag tggcatggaa
    11281 aaagcaatga agcaaatcct cggcgatcaa tcgcagttta ttgccatcga ttttcccgaa
    11341 acctcaagcg ccgcgctgct tacatcgcag cttgaagagg ccatcgccca actggattgt
    11401 gaagacggca ttgtttttct gacggatttg ctgggcggca cgccgtttcg cgtagcttca
    11461 acgctggcga tgcaaaaacc gggctgtgaa gtgattaccg gcaccaattt gcaactgcta
    11521 ctggagatgg tgctggagcg cgaagggtta agcggtgaag aattccgtgt acaggcgctg
    11581 gagtgtggac accgagggct gaccagtctg gtggacgagt taggccgctg ccatgaagaa
    11641 tgtccggtcg aggaaggaat atgacacacg ttctgcgcgc cagaaggctg ctgactgaag
    11701 agggatggtt cgatgaccat cagttgcgtg ttgctgacgg tgtcatcgca gcaatcgaac
    11761 cgattccagt gggcgtgact gaacgcgatg cggaacggct ctgccccgct tacatcgaca
    11821 cccatgtaca cggtggtgcg ggcgttgatg taatggatga cgcgccggat gtactcgaca
    11881 agctggcaat gcacaaggca cgcgaaggtg tcggcagttg gttaccgacc accgtaaccg
    11941 cgccgcttaa taccattcat gcggcgctga aacgtattgc tcaacgttgc caacgcggcg
    12001 gacctggtgc gcaagtgctg gggagttatc tcgaaggacc gtacttcacg ccgcagaata
    12061 aaggcgcgca tccg
//
LOCUS       DAZPEU010000113        12057 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPEU010000113 DAZPEU010000000
VERSION     DAZPEU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.114"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(55..963)
                     /gene="galU"
                     /locus_tag="ACHU2L_002762"
     CDS             complement(55..963)
                     /gene="galU"
                     /locus_tag="ACHU2L_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR1357549.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1165..2178)
                     /gene="rssB"
                     /locus_tag="ACHU2L_002763"
     CDS             complement(1165..2178)
                     /gene="rssB"
                     /locus_tag="ACHU2L_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR1357550.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2270..3175)
                     /gene="rssA"
                     /locus_tag="ACHU2L_002764"
     CDS             complement(2270..3175)
                     /gene="rssA"
                     /locus_tag="ACHU2L_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR1357551.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            3288..3746
                     /locus_tag="ACHU2L_002765"
     CDS             3288..3746
                     /locus_tag="ACHU2L_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR1357552.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3796..4638
                     /gene="purU"
                     /locus_tag="ACHU2L_002766"
     CDS             3796..4638
                     /gene="purU"
                     /locus_tag="ACHU2L_002766"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR1357553.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4798..4882
                     /locus_tag="ACHU2L_002767"
     tRNA            4798..4882
                     /locus_tag="ACHU2L_002767"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4832..4834,aa:Tyr,seq:gta)
     gene            4929..5060
                     /locus_tag="ACHU2L_002768"
     ncRNA           4929..5060
                     /ncRNA_class="other"
                     /locus_tag="ACHU2L_002768"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5244..5921)
                     /gene="narI"
                     /locus_tag="ACHU2L_002769"
     CDS             complement(5244..5921)
                     /gene="narI"
                     /locus_tag="ACHU2L_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1357554.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5921..6631)
                     /gene="narJ"
                     /locus_tag="ACHU2L_002770"
     CDS             complement(5921..6631)
                     /gene="narJ"
                     /locus_tag="ACHU2L_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1357555.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6628..8166)
                     /gene="narH"
                     /locus_tag="ACHU2L_002771"
     CDS             complement(6628..8166)
                     /gene="narH"
                     /locus_tag="ACHU2L_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1357556.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8163..11906)
                     /locus_tag="ACHU2L_002772"
     CDS             complement(8163..11906)
                     /locus_tag="ACHU2L_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR1357557.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         2857 a         3275 c         2969 g         2956 t
ORIGIN      
        1 aaaaaacggc gtcgattgct caacgccgtt tcgtggataa caccgatacg gatgttactt
       61 cttaatgccc atctcttctt caagccaggc tttaaattcc gtgccaaggg tgttatgacg
      121 aataccgtat tcaacgaagg cctgcatgta acctaattta ttaccgcagt catggctctt
      181 ccctttcata tgataggctt ccaccgtttc tttttcgatc agcatatcaa ttgcgtcggt
      241 gagctgaatt tcatcaccag ctcccggagg ggtttttgcc agcaacggcc aaatatccgc
      301 gctaagtacg taacgaccca caatagcgag attagacggc gcaacatccg ctttcggttt
      361 ttctaccaca ccaaccatcg gtacgctttc acccggcgct aattcaacgc ctttgcaatc
      421 cacaacgcca tatgcggtca catcagcaac cggttcaacc atgatctggc tatgacccgt
      481 ttcatcaaag cggcggatca tctctgccag gttatcctgt gacaaatcgg attcatattc
      541 atccagaata acatcaggca aaataacagc taccggttca tcacccacta ccgggtgagc
      601 acacaatacc gcgtgtccca ggcctttcgc cagaccctga cgaacttgca taatagtcac
      661 gtgcggtgga caaatagact gcacttcatc aagcagttga cgttttacac gtttttccag
      721 cattgcttcc agttcaaaac tggtatcaaa gtggttttca atagagtttt tagatgagtg
      781 tgtaaccagc acaatttcag taatgccagc cgcaatacat tcattcacga cgtattgaat
      841 taatggctta tcgacaagtg gcagcatctc tttcgggatg gctttcgtcg ccggcaacat
      901 cctggttcct aatcccgcaa cggggataac ggcttttttg actttcgtat taatggcagc
      961 catttaaatt ctcctggact gttcgtgttt tgaacgtgtt catatttctg tatcgcatcc
     1021 cagtatatca gcaccccgaa ggggctaagt tctgaaaagg aggcgagata gcataattaa
     1081 gacaattact tatacgaatg gcgacgatag taccaccagc cgacattagc aggtaatgca
     1141 aatttagccc gcgttatcgt ttgctcattc tgcagacaac atcaagcgca gtcgaccacc
     1201 ggttccccat atttggcatt gccaggcatc gcatcgctgg ctcaattgat tcaaataagc
     1261 gttacctaaa gtgcctaacg gaacaccatt actgatttgc acctggtgtt cgccggtatt
     1321 taacgtcgca ttcagacccg cagaaaccag aatgagattt ttcagttcgc gatgataata
     1381 gccaactaat agcggaaact gccccggcag attggcctga cgaagtaaat ggtttacctg
     1441 cttcaataac gcgcccaact ctggcaaccg ttgattttgg tgtgcaagct gttcctgtaa
     1501 taatccgtta aacaatgcgc gtaataacaa ggcagcaagt acgccattat gtccagctcg
     1561 ggtgacatca aggcaataaa atgccagatc gttttccgaa agtgcggcaa tatcaagcac
     1621 caggccgggt ttgtccgcgg caaccaattg acggtaatta acccggcaat gggaaatcac
     1681 ctgctgaacc ggcggttgta gttcctgtaa taatttcgcc gctgcggcag ggttatcaac
     1741 cattgcatcc cagtcgcgaa aaagcctttc ctcttcctca acgcgcgaat taaacatgct
     1801 gggatagaga caggcaaaaa ccatctcgcg caagcgattc agatctttaa ctggtttcag
     1861 caaaacatct tcaacgccca gacgtaacgc tttggcaata tctgccatat tttcagtggc
     1921 agatatcacc agaactgggg tctggtcgcc tctgttacgt atatgctcca gcagtttaag
     1981 cccgttcatt cgtggcatcg cgatatcaca tatcatcagg tctggagtga aacctcccag
     2041 caactcaagg gcatccaccc catcagccgc cagtaccgtt gtcgctccca atgaggaaaa
     2101 ccatgaatcc agaagcgagc gaaatacctg ctcatcttca acaatgagaa tctgttttcc
     2161 gaccaatggc tgcgtcatgt tctctcccct gactggcttt actcaatagt ggcatgctat
     2221 tgccacctgc gcctgtcaga attaacttaa gtgtagataa aaattctggt caaatgttgg
     2281 tgcgtaccaa cggcaaaagt tcgtccattt tcctttccac tgccagctgt ccggccgcaa
     2341 tggcagcgtg cgcgcgatgg aaatcaagcg tagatatttg cgggcaaaca ggttgaatca
     2401 gaatatcggg cggatcacct gccatgcggt tccttttaag gcggttctcc aacacctgga
     2461 tagacgtggt cataatctct gttgccgttg gcgctgtcac agcccgacgt gtcgttatgc
     2521 tgcctaacct ttctttcaga cgcgcatgcc acggtagaga atcaccattc tcgctatttt
     2581 cttcactgac attaaaggag agcagatctt gttgcatcaa atgagcatcg tgctgcaggt
     2641 cgaccgctat cacaatatca gcccccaatg cacgcgtaag agaaatagga attgggttaa
     2701 ctactgcccc atcaaccagc cagtagccgt tatgtgcaac gggtgccatg agtcctggaa
     2761 tactgcatga tgcgcgaata gcaagatgga gatcgccttc agtaaaccat aattcacgcc
     2821 ccgtacttaa attggtggca acagccgcaa agcgacggga acaattttcg atctctgttt
     2881 ccggcattat ttcgcgatat tgattgaaga cacgctcgcc gcgcagtaac ccaccgcgct
     2941 gccaggagag gtccatcagg cgtaaaacgt cccaataact gaaagaggtt acccaatctt
     3001 ccagcgcaga taatcgatcg catgcatagg ccgcgcccac cagcgaacca attgaacatc
     3061 ctgcaacgat atcaatttca atacccactt tttttagcgc attaataacg ccaatatgcg
     3121 accaacctct cgccgcgcca gatcccagcg ccagccctat ttttatcttt ctcattattc
     3181 ccgcaagatt cccctggaat gcaatcgtag ccacatcgct actgctcggt taacatagtg
     3241 ctactcaggc ggttaatacg ccgttattgt ttccagggag attatttgtg tctcagcttt
     3301 gtccctgtgg tagtgctgtc gagtatagcc tatgttgcca cccttatgtg tctggtgaaa
     3361 aggttgcacc tgatccagaa catctcatgc gttcgcgtta ctgcgctttt gtgatgcaag
     3421 acgctgatta tttaataaag acctggcatc cctcttgtgg agcagcggcg ttacgtgccg
     3481 aattgatgac cggatttgcg catactgaat ggttgggatt gaccgtattt gaacattgtt
     3541 ggcaggacgc cgacaatatc ggatttgtca gttttgttgc tcgatttacc gaaggtggca
     3601 aaacaggtgc aataattgaa cgttcgcgat ttttgaaaga aaacggtcaa tggtactata
     3661 ttgacggtac acgtccgcag tttggtcgca acgatccctg cccttgtggt tcaggtaaaa
     3721 aatttaaaaa gtgctgcggc caataatggt tgacggtacg gtttagcaaa cactctcaac
     3781 aaggtttttc cagcaatgca ttcactccaa cgtaaagttc tgcgtactat ttgtccggac
     3841 caaaaaggtc tgatcgcacg tattaccaat atttgctaca agcacgagtt aaatatcgta
     3901 cagaacaatg aatttgttga tcaccgtacc gggcgctttt ttatgcgcac ggaactggaa
     3961 gggattttta atgattccac cctgctggcg gatctcgata gcgcattgcc agaaggctcc
     4021 gtgcgtgagc tgaatcctgc cggtcgtcgc cggatagtga ttctggtcac taaagaagcg
     4081 cattgccttg gcgatttgtt gatgaaagcc aattacggcg gtctggatgt cgaaatcgcg
     4141 gcagttattg gtaaccacga tactttacgt tctctggttg agcgttttga tattccgttt
     4201 gagctggtaa gccatgaagg gttaagccgc aacgagcacg atcaaaagat ggcggacgcc
     4261 attgatgctt atcaacctga ctatgtggtg ctggcgaagt atatgcgggt attaacgccg
     4321 gaatttgtgg cacgcttccc gaataaaatc atcaatattc accattcatt cctgcctgcg
     4381 tttatcggcg cacgtcctta tcaccaggcc tatgaacgtg gtgtgaagat tattggcgca
     4441 accgctcact atgtgaatga caatctggac gaaggcccaa tcatcatgca ggacgttatt
     4501 catgtcgatc atacctacac agctgaagat atgatgcgcg caggtcgtga cgtcgagaaa
     4561 aacgtcttaa gtcgtgcgct atacaaagta ctggcgcaac gcgtctttgt ttacggtaat
     4621 cggacgatta ttctttaatc gccagcaaaa ataactggtt acctttaatc cgttacggat
     4681 gaaaattacg caaccagttc atttttctca acgtaacact ttacagcggc gcgtcatttg
     4741 atatgatgcg ccccgcttcc cgataaggga gcaggctagt aaaaagcatt accccgtggt
     4801 ggggttcccg agcggccaaa gggagcagac tgtaaatctg ccgtcatcga cttcgaaggt
     4861 tcgaatcctt cccccaccac catcactttc aaaagccccg gaattctcaa acgaatctgc
     4921 aatcaaatat tctgcccaag cggggaagga tgagaagctt cgaccaaggt tcgacttgag
     4981 cgccagcgag agagcgttgc cgcaggcaac gacccgaagg gcgaagcgcg cagcgctgag
     5041 taatccttcc cccaccacca tcgcttcctt gaataatcaa aacctgttac tccgagctaa
     5101 ttttgcccga tggcaccacg tttaccgggc ctacaatatc ctcccctgta ggtcggataa
     5161 gcgaagcgca tccgacaact caccccgcac cgtgcccata aaaaaacccc gccgaagcag
     5221 ggtctatgtg aactaaaatt cgtttagtga cgagcgcgca ccagctggta cttacgtgtc
     5281 agatactcca ccggtacgct ccagatgtgt accagacgcg agaacgggaa cagcaggaat
     5341 aacgtcatcc ccagcaccag gtgcagacgg aagataaacg ccacaccatc gaggtgttga
     5401 gaagcgccac cgtggaaggt caccaccgac tgcgcccagc caaccagttt catcatctcg
     5461 ctaccgtcca tatgctgagc ggagaacgga atggtcagca ggcccagcgc gcactggata
     5521 acgagcagcg acaggatcag gatatccgct ccggtagtgg ttgcacgcac gcgtgggctg
     5581 aacatacgac gtttcagcaa aagcacgccg ccaatcagac acagcacgcc gctggcacca
     5641 ccagcaaaca tcgccatttt ctgtttcact tcaatcggca gccaggcttc atacatccag
     5701 tgcggcgtca acataccgaa gaagtgaccg acaaaaatcc ccagaatacc gatatggaac
     5761 aggttcgacg ccaggttcat ccctttgcga tccagcatct ggctggacgc cgcgcgccag
     5821 gtgtactgcc cgtagtcata acgcagccag ctaccaatca ggaagaccgc cccggctatg
     5881 tacgggtaga tatcaaagaa gaacatattc aggaattgca ttagtgctgt cctccggtgg
     5941 tgatattcag atattgcggc gcgacggcac cggcaaagcg acgctgatga gcagtgattg
     6001 ctgaatcacc gcagcctttg tcagcaaaga atttaacctg ctcttcttcc cagacagcat
     6061 ccagcgcctg cggcgtatca tcgcgcgctt cgtcggcaat tttttccgcc actttgtcgc
     6121 tgtcgatagc ggtattggcc aatttcagca gcagatcaaa catcacggca taacggcttt
     6181 cacgctgttg cagacgcgcg ctcagcaatg ccagaatcgg cgcgatatct ttcaaacctt
     6241 ccacggcttc gctttgcggc agctgcgaca ggtactccag atacagcggc agatggtccg
     6301 gtaattcgcg gctgtttaac tgcaagccgt gctgctcgta ctgcgccagc aggtccacca
     6361 tcgcctggcc gcggtcacgg gattcaccgt gaacatgttc aaatagcaac agtgacgtgg
     6421 cacggccacg gtcgaacagt tcgctgtact ggacctgggc atcgagcgga tccatcgccg
     6481 ttaaatcgcg caggaaaatg cccagcgcat gggcatcttc tttcgacaga tttttcgacg
     6541 cagcaatcgc ctcaaacatc tcttgttgat gctgccataa ggcagcatcc ggatattcaa
     6601 ggagacgcga tacaatcacg agttcgatca tggatgcggc tccgttttgc tggtcacatc
     6661 gatggcatcg atacgacggc tgttgaacag attgaatttg gtatctgagc cgtggcagcc
     6721 atcaccaaag gtaaagccgc agccattttt ctccgggaag gcttcccgtg ccagttcacg
     6781 atgactactc ggcaccacaa agcgatcttc gtagttagca atcgccagat aacggtacat
     6841 ctcctgtgcc tgggcttcgg tcagaccgac ctcttccagc gcacgggtat caactttacc
     6901 gtcaacagtt tcagcacgtt tgtaatgacg catcgccagc atacgtttca gcgcacgcag
     6961 taccggtttg gtatcaccgg cggtcagcag attcgccaga tactgtaccg gaatacgcaa
     7021 gctttcgacg tctggcagaa ttccgttgct gcccagctcg cccgcgtctg ctgcagactg
     7081 aatcggagac agaggcggca cgtaccagac catcggcagt gtgcgatatt ctggatgcag
     7141 cggcagcgcc agtttccatt ccatcgccat tttataaacc ggcgactgct gtgcagcttc
     7201 aataacgctc agcggaatac cgtctttaat cgcctgctcg atgactttcg gatcgttcgg
     7261 atcgaggaac acgtccagct gacgctggta gagatctttc tcgttctcgg tgctggctgc
     7321 acgttcaatc gcgtcggcat cgtacagcag cacgccaaga taacggatac gaccgacaca
     7381 ggtttctgag cacacggtcg gctgacccgc ttcaatacgc ggatagcaga agatgcactt
     7441 ctcagactta ccgctcttcc agttgaagta gatttttttg tacgggcatc cggtgatgca
     7501 catacgccag ccacggcatt tatcctggtc gatcaggacg atgccatctt cttcacgctt
     7561 gtaaatcgca ccgctcgggc aggtcgccac acatgccggg ttcaggcagt gttcgcacag
     7621 gcgcggcaaa tacatcataa aggtgttttc gaactggcta tacatcgcct tctggatgtt
     7681 gtcgaagttc ttgtctttcg ccagtttatc aaactcaccg cccaggtcat cttcccagtt
     7741 cggccctttt tcgattttcg ccatccgttc cccggtaatc agcgaacgcg gacgggcaat
     7801 cggctgcgat ttgctgcctt ccggcgcggt atgcaggttc tgataatcaa aatcgaacgg
     7861 ctcgtaataa tcgtcgatcc ccggcagatg cgggttagca aagattttac ccagcagcat
     7921 ggctcggtta cccatgcgcg gttgcagttt gccattgatt ttacggatcc agccgccttt
     7981 gtatttttcc tggttttccc agtcagtcgg gaagccctgg cccggcttgg tttccacgtt
     8041 gttgaaccac gcgtattcca cgccttcacg gctggtccag acgtttttac aggtaactga
     8101 acaggtgtgg cagccgatgc acttatcgag attcagcacc atgccgactt gtgaacgaat
     8161 tttcatttta cgctctcctg tacctggtca ttgccttcgc catctaacca gtcaatgttc
     8221 ttcatcttac gcactacaac aaactcatcg cggttagaac ctacggtgcc gtagtagtta
     8281 aagccgtatg ccagatgggc atagccgccg atcatatgcg tcggtttcgg cgtgatacgg
     8341 gtgaccgagt tatggatacc accacgctgt tgggtaattt ccgaaccagg caggttaacg
     8401 atacgttcct gcgcgtggta catcatggtc atccctgccg gaacacgctg gctgacaacc
     8461 gcacgggcag tcagtgcacc gttgctgttg aagacttcaa tccagtcgtt atcggcgata
     8521 cccagatctt tggcatcggc ttcactcaac cagaccaccg gaccaccgcg acctaaagtc
     8581 agcatcagca ggttgtcgct gtaggtggag tggatacccc acttctggtg cggcgtcagg
     8641 aagttgagcg ctttttccgg gttgccgttg gatttctggc ctatcacttc tttcaccgaa
     8701 cgggtgtcga tcggcggacg ataaaccagc aggctttcac cgaaatcacg catccactgg
     8761 tgatcctgat acagttgctg acgaccagag agcgtacgcc atgggatcag ctcgtgaacg
     8821 ttggtgtaac cggcgttgta agaaacgtgt tcatcttcca gaccagacca ggtcgggctg
     8881 gagataattt tgcgcggctg tgcctgaata tcgcggaagc ggatcttctc gtcttcttta
     8941 ttcagcgcca gatgcgtatg gtcacgaccg gtaaattcgc tcagggcagc ccaggctttc
     9001 acggctacct gaccgttggt ttccggtgcc agcgtcagga tcatctctgc cgcatcaatt
     9061 gcggtgttca gcatcggctg gccttttgcc ggaccttccg ctttggtgta gttgagctta
     9121 cgcagcagat ccatctcgct ctgggtgttc caggcaatcc ctttaccgcc gttaccgatt
     9181 ttctccatca gcgggccgat agaggtaaag cgttcgtaag ttgccggata atcgcgctct
     9241 acgaccataa tgtgcggtgc agttttgcct gggatcaggt cgcattcgcc ttttttccag
     9301 tctttcacat ccagcggctg cgccagttca gcggcagagt cgtgctggat aggcagcgtg
     9361 acgatgtcgg tttctttacc cagatagccg acgcacactt cggagaattt cttcgcgatg
     9421 gctttgtaga tttcccagtc gcttttcgct tcccaggccg gatcgaccgc cgcagacagc
     9481 gggtgaataa acggatgcat atccgaagta ttcatgtcgt ctttttcgta ccaggtcgcc
     9541 gtcggcaaaa tgatgtcgga atagagacag gtgctcgaca gacggaagtc cagcgtaacc
     9601 accagatcca gcttgccttc cagaccattg tcctgccagt ccacttcttc cggcttcacg
     9661 ccgccctgtt gccccagatc tttaccctgg ataccgtgct ccgtccccag caggtacttg
     9721 agcataaact catgaccttt accggaagaa ccgagcaggt tagaacgcca gatgaacagg
     9781 ttgcgcgggt ggtttttacc gttttctggt tgttctgccg caaaacggat ggaaccctct
     9841 ttcagggatt tcaccgtata gtccaccgga ttcatcccgg ctttttccgc ttcgccagcg
     9901 atagtcagcg ggttagtgcc taactgcggt gcagacggca gccagcccat gcgttccgca
     9961 cggacgttaa agtcgatcaa gtgtccggta tagcgggatt tgtccgccat cggtgacagc
    10021 aactcttccg ccgtgacggt ttcataacgc cactggctgg agtggttata gaaataagaa
    10081 gtgctgttca tgtgacgcgc cggacgctgc cagtcaaggg caaacgccag cggctgccag
    10141 ccggtttgcg gacgcagttt ttcctgacct acatagtgcg cccagccgcc cccgctctga
    10201 ccgacacagc cgcagaaaat cagcatgttg atcagaccac gatagttcat atcgaggtga
    10261 taccagtggt tcagccccgc accgacgata atcatcgaac gaccgtgcgt tttatcagcg
    10321 ttatcggcaa attcacgggc gatgcgaata atctggctgc gagaaacgcc ggtaatctgc
    10381 tcggcccagg ccggggtata agctttcaca tcgtcatagc tggttgcaca gttaacgtcg
    10441 ttcaggccac gttccagacc gtagtttgcc agcgtcagat cataaacggt ggtcaccagg
    10501 gcggtgctgc catcagccag ttgcaggcgt ttcaccggca gtttgtgcag cagcacgttt
    10561 tccagttcca ctttgttgaa gtgttccgtg ccgtcgccac caaagtacgg gaagcctacc
    10621 tcagcgatct catcctggct acccagcagg ctcagttgca gctcggtttc ttcgccagtt
    10681 ttgccgtcgc gctgctcaag attccatttg cccttctcgc cccagcggaa gccaatagaa
    10741 ccgttcggcg caaccatttc gccattggta ttaaaggcga cagttttcca ttccggattg
    10801 ttttcctggc ccagcgcatc aaccagatca gcagcgcgca gcatacgacc tgcagcgtag
    10861 taaccgtcgc gttcttccag catcaccagc atcggcatgt cggtgtagcg acgcacatag
    10921 tcggtgaaat actggcttgg gttgtcgagg tggaattcac gcagcattac gtggcccatc
    10981 gccagcgcca ttgccgcatc ggtgccctgt ttcggtgcca gccacagatc gcacagtttg
    11041 gcgatttcag cgtagtctgg tgtgacggca acagttttgg tgcctttgta acgcacttca
    11101 gtaaagaagt gagcatccgg ggtacgcgtc tgcggcacgt ttgaacccca ggcgatgatg
    11161 tagctggagt tataccagtc agcagattcc ggtacgtcag tttgctcgcc ccaggtttgc
    11221 ggagacgcag gaggcaggtc gcagtaccag tcgtagaagc ttaagcaagt accgccaatc
    11281 agcgagagat agcgtgcacc cgatgcgtaa gaaaccatcg acattgccgg aattggcgag
    11341 aaaccagcaa cacggtccgg gccgtagttt ttgatggtgt aaacgttaga tgcggcgatc
    11401 agttcgttca cctcctgcca agaagaacga acaaatccac cgcgtccacg cgcctgctta
    11461 aagcttttcg ctttatcggc gtcttcaatg atagaagccc atgcctcaac cggatcgcta
    11521 tgcagcgcct tcgcttcacg ccacattttc atcaggcgtt tgcgcatcat cgggtatttc
    11581 aggcggttgg cactgtaaag ataccaggag tagctggcac cgcgcgggca gccgcgaggt
    11641 tcatggtttg gcagatccgg acgggtacgc ggatagtcag tctgctgggt ttcccaggtg
    11701 accagaccgt ttttgacgta gattttccag ctgcaggagc cggtgcagtt taccccgtgg
    11761 gtagagcgga cgattttgtc atgctgccaa cgctggcgat atccatcctc ccagtcacgg
    11821 ttggtattga gaagctggcc atgcccatcg gcaaaggttt cacccttctg tttgaagtag
    11881 cgaaaccggt ccaggaattt actcatcggt tttctcctgt gggagcctgt cggccctctg
    11941 ataaatcgac attgctaatg tttaagggca aggtaacgct ctgaaacagc gtgggaattg
    12001 ataacgatca agagtgatgg ggatgaaaaa taaagtaaat tcctaagata ccccttt
//
LOCUS       DAZPEU010000114        11972 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPEU010000114 DAZPEU010000000
VERSION     DAZPEU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.115"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            172..942
                     /locus_tag="ACHU2L_002773"
     CDS             172..942
                     /locus_tag="ACHU2L_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR1357558.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            933..1406
                     /locus_tag="ACHU2L_002774"
     CDS             933..1406
                     /locus_tag="ACHU2L_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR1357559.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1513..2052
                     /gene="dnaT"
                     /locus_tag="ACHU2L_002775"
     CDS             1513..2052
                     /gene="dnaT"
                     /locus_tag="ACHU2L_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR1357560.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2055..2792
                     /gene="dnaC"
                     /locus_tag="ACHU2L_002776"
     CDS             2055..2792
                     /gene="dnaC"
                     /locus_tag="ACHU2L_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1357561.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2841..3335
                     /gene="yjjA"
                     /locus_tag="ACHU2L_002777"
     CDS             2841..3335
                     /gene="yjjA"
                     /locus_tag="ACHU2L_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR1357562.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3575..5866
                     /gene="opgB"
                     /locus_tag="ACHU2L_002778"
     CDS             3575..5866
                     /gene="opgB"
                     /locus_tag="ACHU2L_002778"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR1357563.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6004..7026)
                     /gene="lgoD"
                     /locus_tag="ACHU2L_002779"
                     /pseudo
     CDS             complement(6004..7026)
                     /gene="lgoD"
                     /locus_tag="ACHU2L_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7165..8079
                     /locus_tag="ACHU2L_002780"
     CDS             7165..8079
                     /locus_tag="ACHU2L_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR1357564.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8294..9655
                     /locus_tag="ACHU2L_002781"
     CDS             8294..9655
                     /locus_tag="ACHU2L_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1357565.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9704..11368)
                     /gene="tsr"
                     /locus_tag="ACHU2L_002782"
     CDS             complement(9704..11368)
                     /gene="tsr"
                     /locus_tag="ACHU2L_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR1357566.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11487..11933)
                     /gene="hpaR"
                     /locus_tag="ACHU2L_002783"
     CDS             complement(11487..11933)
                     /gene="hpaR"
                     /locus_tag="ACHU2L_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR1357567.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2911 a         3167 c         2912 g         2982 t
ORIGIN      
        1 tttgtttgcg gcgagagata attcgctttt tatcaccgag cttgtcccaa taaccaggat
       61 taatcagcgc aattttgatc ttaacctacc gcaaaacaac cgtgcttcat gatacaaatg
      121 gattctcatt atctgaacaa tacgcagcac gtctatgaca aagggcgagt tatgcaaact
      181 gagcaacagc gagccgtaac gcggctttgt atccagtgtg gattatttct tttgcaacat
      241 ggtgcggaaa gcgcgttggt tgatgagctt tcctcacgac tgggtcgggc gctgggaatg
      301 gacagcgtcg aaagttctat ctcttcgaac gccatagtgc tgacaactat taaagatggg
      361 caatgcctga catcgacacg taaaaatcac gatcgcggca ttaatatgca tgtggtgact
      421 gaagtccagc acattgtgat tcttgcggag catcatctgc tggattacaa aggcgtagag
      481 aaacgattta gccaaattca accattacgt tacccaagat ggctggtagc cttaatggtt
      541 ggcctttctt gcgcctgttt ctgtaaactc aataacggtg gctgggatgg tgccgtcatc
      601 accttctttg ccagtacgac cgcgatgtat atccgccagc tgctggcaca acgtcatctt
      661 catccacaga tcaacttttg ccttaccgct ttcgccgcca ccaccatttc cggattgctt
      721 ttgcaactcc ccactttcag caataccccc accattgcga tggccgccag cgttctgctg
      781 ctagtgccgg gctttccgtt gattaatgcc gtcgccgata tgtttaaagg ccacatcaat
      841 accggactgg cacgctgggc gatcgccagt ctgctgacac tggctacctg cgtcggcgta
      901 gtgatggcac tgacgatttg ggggctacgc ggatgggtgt gatcgaattt ctgttagcgt
      961 tggcgcagga tatgatcctc gccgccattc ctgcggtcgg ctttgcgatg gtgttcaacg
     1021 ttcccgtgcg ggcgttacgc tggtgtgcgc tgcttggctc gataggtcat ggttcccgaa
     1081 tgatcttgat gaccagcggg ttgaatattg agtggtcaac ctttatggct tctatgctgg
     1141 tcggtaccat tggtattcaa tggtcgcgct ggtatctggc gcatccgaaa gtgtttaccg
     1201 tggcggccgt tatccctatg ttcccgggca tatcggctta taccgcaatg atttcgtcgg
     1261 taaaaatcag ccagttaggt tacagcgaac cgttgatgat taccctgtta accaactttc
     1321 ttacagcttc atcgattgtt ggtgcgttat ccatcggtct ttccattcct ggattatggt
     1381 tgtaccgcaa gcgccctcgc gtataaaatt gcctcgctta agcacacgga tgagagacag
     1441 cctcctctcc tccgtgtgtt actataaaag ttatctccct tctcgttcat cgttccatat
     1501 ttgagaaaca gtatgtcttc cagagttttg accccggacg tcgttggtat tgacgccctg
     1561 gtacacgatc accaaaccgt tctggcaaaa gctgaaggcg gtgtggttgc cgtatttgct
     1621 aacaatgccc cggcgtttta tgccgtcacg cctgcacgcc tggctgaact gctggcgctg
     1681 gaagaaaagc tagcgcgtcc gggaagcgat gtcgctctgg acgatcaact ctatcaggaa
     1741 ccgcaagccg ctcccgttgc tgtacccatg gggaaattcg ccatgtatcc ggactggcaa
     1801 cccgatgccg attttatccg cctggcggcg ctatggggcg tggcgctaag agagccggtg
     1861 accaccgaag aactggcctc attcattgcc tactggcagg cggaaggtaa agtctttcac
     1921 catgtgcagt ggcaacaaaa actggcgcgc agcctgcaaa tcggtcgtgc cagcaacggc
     1981 ggactgccga aacgagatgt gaatacggtc agtgaacctg acagccaaat tccaccagga
     2041 ttcagagggt aacgatgaaa aacgttggcg acctgatgca acgcctgcaa aaaatgatgc
     2101 ctgcccatat caaacctgca ttcaaaacgg gtgaagagct actggcctgg cagaaagaac
     2161 aaggggcgat ccgctccgcc gctctcgaac gtgaaaatcg ggcgatgaaa atgcagcgca
     2221 cctttaaccg ctccggtatt cgtccactgc atcagaactg ctcctttgag aactatcgcg
     2281 ttgagtgtga agggcagatg aatgcgttaa gcaaagcgcg ccagtatgtc gaagagtttg
     2341 acggcaacat cgccagcttc atcttttctg gtaagccagg aaccggcaaa aaccatctgg
     2401 cggcggcaat ctgcaacgag ctgctgctac gcggtaaatc cgtattgatc atcaccgtgg
     2461 ccgatattat gtcggcgatg aaagatacct tcaggaatag cggtaccagc gaagaacaac
     2521 tgcttaacga tctgagcaac gttgatctgc tggtgatcga tgagatcggc gtgcagaccg
     2581 aatcgaaata cgaaaaagtg atcatcaacc agatcgtcga tcgccgctct tcttccaaac
     2641 gcccaaccgg gatgctgacc aacagtaata tggaagagat gaccaagttg ctgggcgaac
     2701 gcgtgatgga ccgtatgcgc ctgggtaaca gtttatgggt gatcttcaac tgggatagct
     2761 accgtagccg ggtaacgggt aaagagtatt aatccccgtt tctggcggta tactgcgaca
     2821 caattccacc ttgagtaatg atgatgaaaa ctattaaaca tcttctgtgc tgtgccattg
     2881 ccgccagcgc attaatttcc accggggtgc atgctgcgtc ctggaaagat gcgctctcca
     2941 gcgccgccag cgaacttggc aaccaaaaca gcacgacaca ggaaggcggt tggtcgctcg
     3001 cgtcattaac taacttgctt agcagcggaa accaagcctt aagcgcagat aacatgaaca
     3061 atgccgcagg cattctgcaa tactgcgcga agcaaaagct ggcttcggta accgatgccg
     3121 aaaacatcaa gaaccaggtg ctggaaaagc tgggcctgaa cagtgaagag caaaaagaag
     3181 acaccaacta tctggacggt attcagggtt tgctgaaaac aaaagatggt cagcaactca
     3241 atctggataa catcggaacg actccgctgg cagaaaaggt gaaaaccaaa gcctgcgatc
     3301 tggtgttaaa gcaggggctg aacttcattt cctgaaccgc gaaatgttac ccggctgacg
     3361 cggctctctc ctgccctttt ccctgctcct ggacggtttt ccccctgtta cacgggaagc
     3421 gaccgcgatc aaaaaagtct gtttataaac taattcctaa caacatcgca tttttttgac
     3481 actagaattg cagcaatatg gcgacgccat tacattgtgc ttctcaaaaa gcttaaacgt
     3541 tggctaaacg gcccggttta gtgaggatcg cccattgtca gaactactct ctttcgccct
     3601 ttttctcgcc tctgtgctga tttacgcatg gaaagcggga cgtaacacct ggtggtttgc
     3661 agccacgtta acggtgctgg ggctatttgt cgttttaaat atcaccctgt ttgccagcga
     3721 ctattttact ggcgatggta ttaacgacgc ggttctctat accttaacca acagcctgac
     3781 cggtgctggc gtcagcaaat acatcctgcc gggtatcggc attgtgctgg ggctgacggc
     3841 ggtgttcggt gcgctgggct ggatcctgcg ccgtcgtcgt catcatccgc accattttgg
     3901 ttacagcctg ctggcgctct tactggcgct gggttcggtg gacgccagcc cggcatttcg
     3961 tcagataacg gaactggtga aatcccagtc acgcgatggc gacccggact ttgcggctta
     4021 ttataaagag ccgtcgaaaa ctatccctga cccgaaactc aacctggttt atatctacgg
     4081 cgaaagtctc gagcggacct attttgataa cgaagctttc ccggatctca cgcctgaact
     4141 gggcgcgttg aaaaatgaag gcctggattt cagccacacg cagcagctgc caggaacgga
     4201 ttacacgatt gcgggcatgg tggcttctca gtgcggcata ccgctgttcg ccccctttga
     4261 aggcaacgcc tccgcctctg tctccagctt cttcccgcag aacatctgtc tgggcgatat
     4321 cctgaaaaac tcgggttatc agaactattt cgtgcagggc gcaaatctgc gttttgccgg
     4381 taaagatgtg ttcctgaaat cgcacggctt cgaccactta tacggctcag aagagctgaa
     4441 aagcgtggta gccgacccgc actatcgcaa cgactgggga ttctacgacg ataccgttct
     4501 cgatgaagcg tggaaaaagt ttgaagagct ttcccgctca ggtcagcgat tctcactgtt
     4561 taccctgaca gtcgataccc atcacccgga tggttttatc tcgcgcacct gtaaccgcaa
     4621 aaaatatgat tttgacggta agccgaatca gtcattcagc gcggtaagtt gcagccagga
     4681 gaatatcgcg acgtttatca acaaaatcaa agcgtcaccg tggtttaaag atactgtcat
     4741 cgtcgtctct tctgaccatt tagcgatgaa caacacggcg tggaaatacc tcaataagca
     4801 ggatcgcaat aacctgtttt ttgtcattcg tggcgacaag ccgcagcaaa agacgctggc
     4861 agtgaagcgt aacacgatgg ataacggcgc gacggtgctg gacattctcg gtggcgataa
     4921 ctatctcgga cttggtcgta gcagtttatc cgggcagtcg atgtcggaaa tcttcctcaa
     4981 tatcaaagag aaaacattgg cgtggaagcc agatatcatc cgcctgtgga aattccctaa
     5041 agagatgaaa gagttcacca tcgaccagca gaaaaacatg attgccttct cgggtagcca
     5101 tttccgtttg ccgctgctgt tgcgggtttc agacaaacgc gtggaaccgc tgccggaaag
     5161 cgaatactca gcaccgctgc gtttccagct ggccgatttc gctccacgcg acaatttcgt
     5221 ctgggttgac agttgctaca agatggcaca actctgggct ccggaactgg cactttccac
     5281 cgactggtgt gtctcgcagg ggcagcttgg cggtcagcaa attgttcagc atgttgacaa
     5341 aacaacatgg aagagcaaga cggcatttaa agatacggtc atcgacatgg cgcgttacaa
     5401 aggcaatgtc gatacgctga agattgttga taacgatatt cgctacaaag ccgacagttt
     5461 catctttaac gtcgccggtg cgccagaaga ggtgaaacag tttagcggga tttcccgtcc
     5521 ggagtcgtgg ggccgctggt ccaacgcgca gctgggcgat gaagtaaaaa tcgagtacaa
     5581 gcatccgctg ccgaagaaat ttgacctggt gattaccgcc aaagcatacg gcaataacgc
     5641 cagccgtcct attccggtac gcgtaggcaa tgaagaacaa actcttgtgc tgggcaatga
     5701 agtgaccacc accacactgc atttcgataa cccaaccgat gccgacacac tggtaattgt
     5761 gccgccggaa cctgtctcaa ccaacgaagg gaatatcctc ggacactcgc cgcgtaagct
     5821 cgggatcggc atggtggaaa ttaaagtggt agaacgtgaa gggtaattcc tgactgatgt
     5881 gtaggtcgca tagggtgcga cctacacgcc ttctccggct tacaacacat tattaagaaa
     5941 caaaaaaaac agcgcccaac acttccccgg acgctgcatg gcaactttaa taatctcgcc
     6001 ttatcagaaa gtaattacac ccttaatcaa ctcacgattg ttaatcacat cgcgctcgta
     6061 ggtttctgcc agcgtggcga acggatagcg atgggttaac atcatgtcgg cagtgatttt
     6121 cccttccgcc atcagtcgac cgactttagc gaaatcttcc ggcgtggcgt tgcggctgcc
     6181 catcatcgtc gtttcttttt tatggaattc cggatcggag aactgcaact cacctttaaa
     6241 cagaccgaca aataccaccg tgccgccgtg acgaatcaga ttcacggtat tattcatcgc
     6301 atgttgatta cctgtcgcgt cgatcacttt ctgcgccagc gaaccaccaa actgcgcccg
     6361 cagctgcgcg tcaaaatctt cggctgacgg gtccagcacg ggcaactcca gacgcgttgc
     6421 cacatgttca cggcgcgccg gactggtatc cgccaccacc acctgtgcgc catcggcttt
     6481 agcgattgcc gccgcgccca gaccgattgg ccccgccccg accaccagca cctgctcgcc
     6541 gggagcaatg gctgcgcgac gcaccgcatg agcgctaata gcgaaaggtt caatcaatgc
     6601 ggccgcctgc gggtcaatac cgtctgcggg caaaatgttc gccaccggca cgctcaaata
     6661 ctcactaaaa ccgccatcct gatgcacgcc aatgactgaa attttttcac agcagttggt
     6721 acgcccgctt ttacacgccg ggcattgctg acaggcaaca taagggatca cagcaacttg
     6781 ctgaccattt ttaagatcag caatattttt acccagccca acaatctccc cacatatttc
     6841 atggcctaaa acccgtggat aactaaaaaa tggttgattt ccaccctagg catgaatatc
     6901 ggtaccgcaa atcccgacag acttaatttt tattaatgct tcattgtcac ccggaatagg
     6961 tatctcgcgt tgtttccaga ctaattcttt cggctgctgg caaattaaaa cattcatcgt
     7021 agacatgatt cggctatcca ttttttgttg ccttagatat caatgaaaat gaaaaatggc
     7081 agcggaacgg aaaatctgtt ttgtgaaaac acacgcataa aaatgtttta aatcgggttt
     7141 taatggactc acaaggagga aaatatgagt cgttcacaaa atttacgcca caatgtgatt
     7201 aaccaggtca ttgatgatat ggcgcggggt catatccctt ccccacttcc atcgcaaagt
     7261 gcgctggcgg aaatgtacaa catcagccgc actacagtgc gtcatattct cagccaatta
     7321 cgcgaatgcg gcgtcttaac gcaggttgga aacgactatg ttattgcgcg taaacctgac
     7381 catgatgacg gtttcgcctg tactaccgct tcaatgagtg aacaaaacaa agtgtttgag
     7441 caggcttttt tcacgatgat aaaccagcgt cagttacgcc ctagggaaac tttttctgaa
     7501 ctgcaactgg ctcgggccgc gggtgtcagc ccggtggttg taagagaata tcttttaaaa
     7561 ttcggccgtt ataatcttat tcaaagcgaa aagcgcggcc agtggagcat gaagcaattc
     7621 gatcaatcct acgccgagca gctgtttgag ctacgcgaaa tgctcgaaac acattcactt
     7681 cagcactttc tcaacctgcc tgatcatgat ccccgctggc tgcaggcgaa aaccatgcta
     7741 gaacgtcatc ggctactgcg tgacaacatc ggcaatagct ttcgcatgtt ctcgcaactt
     7801 gacagagatt ttcactcctt actgctttca gctgccgaca atatcttttt tgatcaatcg
     7861 cttgagatca tctcagtcat ctttcatttt cactatcaat gggacgaaag cgatcttaaa
     7921 cagcgcaata tcattgccgt cgatgaacat atgaccattc tcagcgctct gatttgccgc
     7981 agtgaccttg atgccaccct tgcgctccgt aaccatttga attcagctaa acaatcaatg
     8041 attcgatcca ttaatgagaa cacgcgttat gctcattaag tacggattaa aaacagcaaa
     8101 ctattagcgg gtgcacggtt ttatcaaaag ataaataccg ggaaattccc gcttacctat
     8161 gctcacaatc agtcgactaa aaatgttttt gtgagcggta gtaaagtcct aaaactttaa
     8221 cctgccgtta atgtcaacct tgcgtaatgc acaattacgt aaacaattaa aattataaat
     8281 acaggagtcc ggcgtggaaa aagaaaatat caccctcgat ccgcgttctt catttactcc
     8341 atcttcgtcg gcagatattc ccgtgccacc agatggatta gttcaacgca gtacccgaat
     8401 taaacgcatt caaaccaccg ccatgttgtt attatttttt gcggcggtaa tcaattatct
     8461 cgaccgcagt tcgctgtcgg tagcaaattt aacgattcgt gaagaattgg gattaagtgc
     8521 caccgaaatc ggcgctttgc tctccgtgtt ttcactcgct tacggcattg cgcaacttcc
     8581 ttgcggccca ctattggatc gtaaaggccc acgcctgatg ctgggactgg ggatgttctt
     8641 ctggtcactg ttccaggcca tgtctggcat ggtgcacagc tttacgcagt tcgtgttggt
     8701 gcgtatcggt atggggattg gtgaagcgcc gatgaaccca tgcggtgtaa aagtcattaa
     8761 cgactggttc aacatcaaag agcgcggacg cccgatgggc ttcttcaacg cagcttctac
     8821 cattggcgtt gctgtaagcc caccgattct ggcggcaatg atgctggtga tgggctggcg
     8881 cgggatgttt attaccattg gtgtactggg gatttttctc gccatcggct ggtatatgct
     8941 ctatcgcaac cgcgagcacg tagaactgac tgccgttgaa caagcttatc tcaatgcagg
     9001 tagcgtcaat gcccgccgag atccgctcaa ttttgccgaa tggcgcagcc tgttccgtaa
     9061 ccgcacaatg tggggaatga tgctcggatt cagtggcatc aactacactg cgtggctgta
     9121 tctggcctgg cttcctggtt acctgcaaac agcctataac ctggatttaa aaagcacagg
     9181 gttgatggcg gctatccctt tcctgtttgg ggctgccggg atgctggtca acggttacgt
     9241 tactgactgg ctggtcaaag ggggaatggc tccgattaaa agccgtaaga tctgcattat
     9301 taccgggatg ttctgttctg ccgcctttac gctgatagta ccacaagcga caacatccat
     9361 gatggcggtt ctgctgattg gcatggcact gttctgtatt cactttgccg gaacatcctg
     9421 ctggggcttg atccacgtcg cagttgcttc tcgcatgact gcgtcggtgg gcagtatcca
     9481 gaactttgcc agcttcatct gcgcctcttt cgcgccgatc attactggtt ttattgttga
     9541 tacaacccac tcattccgtc tggcactaat catttgcggt tgcgtcaccg cagcgggagc
     9601 actggcgtac atcttcctgg ttcgccagcc gatcaacgac ccgcgtaaag attaaactcc
     9661 ctatgcccga taacattttg cttatcgggc atttttatgg cgattaaaac gtttcccagt
     9721 tctccccgct atctgccact gccattttac gcggcgtagc tggcgtcacg gtttttacca
     9781 cagccgatgt ttcacgctgc tgttgctgct gttgctgaat ccggaacact gccacagctt
     9841 cggtcaggcg actggcctgc tcttccagcg cggcggcggc agcggcagat tcttccacca
     9901 gcgcggcgtt ctgttgagtt acccggtcca tctcagcaac cgctaagcca acctgatcga
     9961 taccacggct ctgctcatca gaagcagaag ctatttcgcc cataatgtcc gtcacgcggg
    10021 tcacagcact gacaatctcc gccattgttt ccccggcgct ttcgaccagc gtagagccaa
    10081 catccacttt ccccaccgag tcttcaatca ggcttttaat ttcacgaacc gcctgggcgc
    10141 tacgctgggc cagattacgc acttctcccg caaccaccgc aaaaccacgt ccttgctcac
    10201 ccgcacgcgc tgcttcaacc gccgcgttca aagccagaat attggtctgg aaggtaatgc
    10261 cgtcaattac gctgataata tcggcgattt tctgcgaact ggtggagata tcgcgcatag
    10321 tctgcaccac gttatctacc actttgccgc cgcgttgcgc cgtttcagaa gcacttaatg
    10381 ccagatggct ggcctggcgc gcattctcgg cgttctgttt caccgttgcg gtcagttgct
    10441 ccatgctggc tgccgtctct tccagcgaag cggcctgttg ctcggtgcgc gaagagagat
    10501 cattattgcc ggtagcgatt tcgctggcac cgctatagat ggcattggcc ccgttgcgca
    10561 catcaccgac ggtacgcatc agctctccct gcatatggcg caaactctct gccagttgcc
    10621 ccatctcatt agagccatcc acctcaatcg gtttcaccag atcgccgcct gcaatatgac
    10681 gaatgctgtc aatcaggcga ttcattggcg ctaccagcga ggctttaata ccgaaccaga
    10741 cggcgaagat gaccgccagt acgacgatca tcacgcccac cagaatccac atcgcctggc
    10801 tgtaggaggc attgttatcg ctgacggcga tatcatagag ccgatcgttt tgctccatgt
    10861 aagccacata ctgcttctcg aaaccgtcct gatatccctg ggtcggctga tcaaagaact
    10921 cgttgatttt gcctgcacct aacagttgga tcagctccgc cagcgcattg tgataaatat
    10981 cgtaattacg tttgatctct gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg
    11041 cttcgtaatc cgcccagttt ttttccgcct gtttcagcga aatactggca ctctgcatca
    11101 gctcagcaac ggttgaaccg ctaccaatat tattctgatc catcatgtag cggatacccg
    11161 cgcggttgag ggtgttacgc gtctgcaaca acgcgaccca gctgccattc agcgtgggtt
    11221 gctgctggcg aatggtttgt aaaacagtga aattttcttt gtcattcttt aaggcattaa
    11281 aaaacagacc gcctgatgtc agttgtaaaa ggccaaaaac ggccaaaacc agcagtaagc
    11341 tggtcacgat cttgatacgt tttaacatgg tttctctttc ctgtggaccg atgcaagatt
    11401 ttcggcctgg aaagcaaaaa ctttatgaaa aggtgtaacc cgccatccat ggaaaaacca
    11461 ggcgctggtt atcagatact aaaaagttat tcattatcgc ccgggatgtc ttcttgtcgg
    11521 gaattgccca gagcaataaa ttcctctaac agatgggtta actgttgcat tttctcggca
    11581 gtaaactgag cttcaatctg ccgataggct tcttcaatct gtgtctgagc gcggttatac
    11641 agcgcctgcc cctctttggt cagcgagata tagagcttcc gctgatcatt gatcggcttt
    11701 aatcgcaaca ctaaaccgtc gcgttccatc cgcgtgagga tcccggtcag gcttgggcgc
    11761 aaaatgcagg cgcgatacgc cagatcgtga aaatccattg atgggctttc cgccagaata
    11821 cgcacgatgc gccactgttg ctcagtcaaa ttatgccgtt taacgattgg gcgaaaataa
    11881 ctcatcgccg cttctcgcgc ctggagcaac gcaatggtta gtgagtcgtg cattatcttt
    11941 cccctgaaaa attcaaaatc attaatatag aa
//
LOCUS       DAZPEU010000115        11902 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPEU010000115 DAZPEU010000000
VERSION     DAZPEU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.116"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..2251)
                     /locus_tag="ACHU2L_002784"
     CDS             complement(<1..2251)
                     /locus_tag="ACHU2L_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFR1357568.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="ACHU2L_002785"
     CDS             complement(2275..2898)
                     /locus_tag="ACHU2L_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR1357569.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="ACHU2L_002786"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="ACHU2L_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHU2L_002787"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHU2L_002787"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR1357570.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHU2L_002788"
     CDS             7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHU2L_002788"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR1357571.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..11902)
                     /gene="yfaL"
                     /locus_tag="ACHU2L_002789"
                     /pseudo
     CDS             complement(8356..11902)
                     /gene="yfaL"
                     /locus_tag="ACHU2L_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2753 a         3298 c         2940 g         2911 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag gccccctgct tctgattacc
     8881 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
     8941 agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac caaccgcccc
     9001 aagcatccac tcgccatccg tgccccagcg cccgctaaac aagccgccgc taagctgcac
     9061 cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
     9121 gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc gctccatcat
     9181 aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg cattcagcac
     9241 cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg gcgtgggatc
     9301 aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt gcgatcgcag
     9361 ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt tgacgtagcc
     9421 actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag ctgcgaaatc
     9481 aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt taaccaccac
     9541 agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg agtcatcgcc
     9601 gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt ttaccgtcag
     9661 cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg tcagatcacc
     9721 attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc
     9781 gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt taaatagtga
     9841 ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac catcgctgat
     9901 gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg cgccagtaac
     9961 gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat ccgcaacatc
    10021 gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag
    10081 ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat gctgcccgct
    10141 gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc
    10201 gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt
    10261 ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct ggcggttata
    10321 gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg cgatttgtgt
    10381 tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg catcgaccac
    10441 cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac ctgcaagatc
    10501 taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa ggccatcatt
    10561 gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac cgccgtttaa
    10621 cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga
    10681 aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact gcgcccctgc
    10741 cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tgatgccagc
    10801 aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag tgccattctg
    10861 aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta gctcagcctg
    10921 attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg
    10981 gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt
    11041 ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga
    11101 acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt cattctctgt
    11161 attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag
    11221 atacataaag ccacccgccg cagtggaggg accatctcca tagcctgctg cgctattgtt
    11281 ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag
    11341 atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg ctgtgttatt
    11401 tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt cgctattatt
    11461 atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg tatatgcctg
    11521 gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat aaattgcgcc
    11581 acctttgcca tcattagcga tattattgat ggcgttagtg acacgtaaat cgacggcacc
    11641 agtatcatta gtaccagaag aatagattgc gccaccatag ccgcctgcga cgttaccgga
    11701 aaaaataaca tcagtaagat tcagcgttga gttttcttta gcaaatatgg ccccgccgtt
    11761 attatattct cctgtaacgg tgttattggc aaacagagtc attccagttt cattttctgg
    11821 taatagtgaa aattccgctc cttgttgcaa aaatacggcc ccaccgctgg cattattggt
    11881 catatccgaa aaaaccagcc at
//
LOCUS       DAZPEU010000116        11802 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPEU010000116 DAZPEU010000000
VERSION     DAZPEU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.117"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..851
                     /locus_tag="ACHU2L_002790"
     CDS             <1..851
                     /locus_tag="ACHU2L_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR1357572.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACHU2L_002791"
     CDS             873..2282
                     /locus_tag="ACHU2L_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1357573.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACHU2L_002792"
     CDS             2260..3039
                     /locus_tag="ACHU2L_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR1357574.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACHU2L_002793"
     CDS             3036..3896
                     /locus_tag="ACHU2L_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1357575.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="ACHU2L_002794"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="ACHU2L_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="ACHU2L_002795"
     CDS             complement(4636..4896)
                     /locus_tag="ACHU2L_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1357576.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="ACHU2L_002796"
     CDS             complement(4887..6158)
                     /locus_tag="ACHU2L_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1357577.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHU2L_002797"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHU2L_002797"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR1357578.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHU2L_002798"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHU2L_002798"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR1357579.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="ACHU2L_002799"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="ACHU2L_002799"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR1357580.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="ACHU2L_002800"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="ACHU2L_002800"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1357581.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="ACHU2L_002801"
     CDS             11500..11652
                     /locus_tag="ACHU2L_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1357582.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2843 a         2994 c         3241 g         2724 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcattaattt attcataatg ta
//
LOCUS       DAZPEU010000117        11793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPEU010000117 DAZPEU010000000
VERSION     DAZPEU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.118"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            38..778
                     /locus_tag="ACHU2L_002802"
     CDS             38..778
                     /locus_tag="ACHU2L_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1357583.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACHU2L_002803"
     CDS             837..1223
                     /locus_tag="ACHU2L_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1357584.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACHU2L_002804"
     CDS             1232..1561
                     /locus_tag="ACHU2L_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1357585.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACHU2L_002805"
     CDS             1533..4574
                     /locus_tag="ACHU2L_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1357586.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACHU2L_002806"
     CDS             4574..4903
                     /locus_tag="ACHU2L_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1357587.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACHU2L_002807"
     CDS             4903..5601
                     /locus_tag="ACHU2L_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1357588.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACHU2L_002808"
     CDS             5606..6349
                     /locus_tag="ACHU2L_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1357589.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6313..6888
                     /locus_tag="ACHU2L_002809"
     CDS             6313..6888
                     /locus_tag="ACHU2L_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1357590.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="ACHU2L_002810"
     CDS             6949..10428
                     /locus_tag="ACHU2L_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1357591.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="ACHU2L_002811"
     CDS             10496..11095
                     /locus_tag="ACHU2L_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1357592.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11159..>11793
                     /locus_tag="ACHU2L_002812"
     CDS             11159..>11793
                     /locus_tag="ACHU2L_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1357593.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2774 a         2795 c         3790 g         2434 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggctgcagc
    11521 cggaaacgca cagcagacgg cgcaggatgt ggcggcagcc gcaacggccc gtgatgatgc
    11581 acaacgtttt gcggagaaag cccgacagga tgcaaccgtc acagctgagg acagaaaggc
    11641 cactgcggaa gatgtgacaa gcacaggagc aaatgcagcc gcagccggac agagcgcaca
    11701 ggatgccgca ggttatgccc gcgcagcaga acaggccaaa aatgacattg atgctgcgct
    11761 gaccggcact ctgaaaatgg ctaaccatct gtc
//
LOCUS       DAZPEU010000118        11655 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPEU010000118 DAZPEU010000000
VERSION     DAZPEU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.119"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..794
                     /locus_tag="ACHU2L_002813"
     CDS             <1..794
                     /locus_tag="ACHU2L_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR1357594.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHU2L_002814"
     CDS             complement(744..1937)
                     /locus_tag="ACHU2L_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1357595.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACHU2L_002815"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACHU2L_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR1357596.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACHU2L_002816"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACHU2L_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR1357597.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACHU2L_002817"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACHU2L_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR1357598.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACHU2L_002818"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACHU2L_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR1357599.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACHU2L_002819"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACHU2L_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR1357600.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="ACHU2L_002820"
     CDS             complement(10573..10716)
                     /locus_tag="ACHU2L_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357601.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11655
                     /locus_tag="ACHU2L_002821"
     CDS             10955..>11655
                     /locus_tag="ACHU2L_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR1357602.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DAZPEU010000119        11596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPEU010000119 DAZPEU010000000
VERSION     DAZPEU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.120"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..2264
                     /locus_tag="ACHU2L_002822"
                     /pseudo
     CDS             1..2264
                     /locus_tag="ACHU2L_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2265..3914
                     /locus_tag="ACHU2L_002823"
     CDS             2265..3914
                     /locus_tag="ACHU2L_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR1357603.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="ACHU2L_002824"
     CDS             3919..4695
                     /locus_tag="ACHU2L_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR1357604.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHU2L_002825"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHU2L_002825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR1357605.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHU2L_002826"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHU2L_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR1357606.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHU2L_002827"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHU2L_002827"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR1357607.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2790 a         2823 c         3123 g         2860 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataatat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagctga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttgccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattat
//
LOCUS       DAZPEU010000120        11392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPEU010000120 DAZPEU010000000
VERSION     DAZPEU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.121"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACHU2L_002828"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACHU2L_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHU2L_002829"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHU2L_002829"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1357608.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHU2L_002830"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHU2L_002830"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR1357609.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHU2L_002831"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHU2L_002831"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR1357610.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHU2L_002832"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHU2L_002832"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR1357611.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHU2L_002833"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHU2L_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR1357612.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="ACHU2L_002834"
     CDS             complement(8300..11167)
                     /locus_tag="ACHU2L_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1357613.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         2742 a         2807 c         2845 g         2998 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc cccttacaaa ggtgagttta tatcgagtta ttccctaagt tg
//
LOCUS       DAZPEU010000121        11345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPEU010000121 DAZPEU010000000
VERSION     DAZPEU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.122"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            240..539
                     /locus_tag="ACHU2L_002835"
     CDS             240..539
                     /locus_tag="ACHU2L_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR1357614.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(659..838)
                     /locus_tag="ACHU2L_002836"
     CDS             complement(659..838)
                     /locus_tag="ACHU2L_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR1357615.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            912..2273
                     /gene="pabB"
                     /locus_tag="ACHU2L_002837"
     CDS             912..2273
                     /gene="pabB"
                     /locus_tag="ACHU2L_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR1357616.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2277..2855
                     /locus_tag="ACHU2L_002838"
     CDS             2277..2855
                     /locus_tag="ACHU2L_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR1357617.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            3039..4403
                     /gene="sdaA"
                     /locus_tag="ACHU2L_002839"
     CDS             3039..4403
                     /gene="sdaA"
                     /locus_tag="ACHU2L_002839"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1357618.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4534..6132
                     /locus_tag="ACHU2L_002840"
     CDS             4534..6132
                     /locus_tag="ACHU2L_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1357619.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6136..7686)
                     /gene="yoaE"
                     /locus_tag="ACHU2L_002841"
     CDS             complement(6136..7686)
                     /gene="yoaE"
                     /locus_tag="ACHU2L_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR1357620.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7674..7832)
                     /gene="yoaL"
                     /locus_tag="ACHU2L_002842"
     CDS             complement(7674..7832)
                     /gene="yoaL"
                     /locus_tag="ACHU2L_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR1357621.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8149..9120
                     /gene="manX"
                     /locus_tag="ACHU2L_002843"
     CDS             8149..9120
                     /gene="manX"
                     /locus_tag="ACHU2L_002843"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR1357622.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9183..9983
                     /gene="manY"
                     /locus_tag="ACHU2L_002844"
     CDS             9183..9983
                     /gene="manY"
                     /locus_tag="ACHU2L_002844"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR1357623.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9996..10847
                     /gene="manZ"
                     /locus_tag="ACHU2L_002845"
     CDS             9996..10847
                     /gene="manZ"
                     /locus_tag="ACHU2L_002845"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR1357624.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10902..>11345
                     /locus_tag="ACHU2L_002846"
     CDS             10902..>11345
                     /locus_tag="ACHU2L_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR1357625.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         2785 a         2866 c         2852 g         2842 t
ORIGIN      
        1 cgccctttga ttagcaactc tggttttctt tcttagctga acaatctggt tatagcgtgc
       61 gggctgaccg ttaattttct ccatcttact ggataacaca ccatgagctg ctacctttaa
      121 aacacctgaa tgtcaacagg ttaactcgtg ccatatcgtt ataatccctt tgatattaga
      181 tgcaaattaa ggtcatatat agccttatcg ataacatggt taattttaag gataagaata
      241 tgcctgctgt aatagataaa gccctggatt tcattggtgc catggatgta tcagcgccaa
      301 caccaagttc gatgaatgaa agcacggcga agggaatctt taaatattta aaagaactgg
      361 gagtacccgc gagtgccgct gatattacgg cgcgagctga ccaggaaggc tggaaccctg
      421 ggttcacgga aaaaatggtt ggatgggcaa aaaaaatgga gacaggtgaa cgctctgtga
      481 ttaaaaatcc tgaatacttt tcaacatata tgcaggaaga actaaaagca ctggtctgag
      541 ttaaatttat atcagcataa atgggtcagg acgcttttaa tcacatataa aaagcgtcct
      601 ttttctcaca atcaaatgat catcggcttc tataacgaaa tcgatacgga ttagtgtgtt
      661 actcgtcttc atcctcaaaa cgcgccacga ttagctcgcc ggagtggttg gcacgcagct
      721 cttctgccac cagcgcgatt gcctggccgc tactcatccc ttgtgccatc agttcctgga
      781 tccgctcgac agctttttgc tgctgttcat ggatgagtga aggtaaacct gcaaacattg
      841 ttaactcctg ctaaattgtt ggcgctaatt atttcatgct acccggcaca tagccagtag
      901 agtcaggact gatgaagacg ttatctccca ctgtgattac tttaccctgg cgtcaggacg
      961 ccgctgaatt ttatttctcc cgcttaagcc acctgccgtg ggcgatgctt ttacactccg
     1021 gctatgccga tcatccgtat agccgctttg atattgtggt cgccgatccg atttgcactt
     1081 taaccacttt aggtaaagaa accgttgtta gtgaaagcga aaaacgcaca acgaccactg
     1141 atgacccgct acaggtgctc cagcaggtgc tggatcgcgc agacattcgc ccaacgcata
     1201 acgaagattt gccatttcag ggcggcgcac tggggttgtt tggctacgat ctgggccgcc
     1261 gttttgagtc actgccagaa attgcggaac aagatatcgt tctgccggat atggcagtgg
     1321 gtatctacga ttgggcgctc attgtcgacc accagcgtca tacagtttct ttgctgagtc
     1381 ataatgatgt caatgcccgt cgggcctggc tggaaagcca gcaattctcg ccgcaggaag
     1441 atttcacgct cacttccgac tggcaatcca atatgaccca cgagcagtac ggcgaaaaat
     1501 ttcgccaggt acaggaatat ctgcacagcg gtgattgcta tcaggtgaat ctcgcccagc
     1561 gttttcatgc gacctattct ggcgatgaat ggcaggcatt ccttcagctt aatcaggcca
     1621 accgcgcgcc atttagcgct tttttacgtc ttgaacaggg tgcaatttta agcctttcgc
     1681 cagagcggtt tattctttgt gataatagtg aaatccagac tcgcccgatt aaaggcacgc
     1741 taccacgcct gcccgatcct caggaagata gcaaacaagc agaaaaactg gcgaactcag
     1801 cgaaagatcg tgccgaaaat ctgatgattg tcgatttaat gcgtaatgat atcggtcgtg
     1861 ttgccgtagc cggttcggta aaagtaccag agctcttcgt ggtggaaccc ttccctgccg
     1921 tgcatcatct ggtcagcact ataacggcgc gactaccaga acagttacac gccagcgatc
     1981 tgctgcgcgc agcttttcct ggtggctcaa taaccggggc tccgaaagta cgggctatgg
     2041 aaattatcga cgaactggaa ccgcagcgac gtaatgcctg gtgcggcagc attggctatt
     2101 tgagcttttg cggcaacatg gataccagca ttactatccg cacgctgact gccattaacg
     2161 gacaaatata ctgctctgcg ggcggtggaa ttgtcgccga tagccaggaa gaagcggaat
     2221 atcaggaaac ttttgataaa gttaataaga tattacgcca actggagaag taagacgtgg
     2281 aataccgtag cctgacgctt gatgattttt tatctcgctt tcaacttttg cgcccgcaaa
     2341 ttaaccggga acccctaaat catcgtcagg ctgccgtgtt aatccccatc gtccgtcgac
     2401 cgcaaccggg gttgttgctg actcagcgtt caattcatct gcgtaaacac gctggacaag
     2461 tggcattccc tggaggtgca gtcgatgaca cggacgcatc agttatcgcc gccgcgctgc
     2521 gcgaagctga agaagaggtc gctataccgc cttccgccgt tgaagttatc ggcgtgctgc
     2581 cgcccgtcga tagcgtaact ggctatcaag taaccccagt ggtcggcatt atcccgcccg
     2641 atctgccgta tcgcgccagt gaagatgaag tctcggcggt gtttgaaatg ccgctcgccc
     2701 aggcattaca tctgggtcgt tatcaccctt tagatatcta ccgccgtggc gattcacatc
     2761 gggtatggct gtcctggtac gaacagtatt ttgtatgggg aatgaccgca ggcataattc
     2821 gtgagctggc gctgcaaatt ggtgtgaccc cctgactata cttatcttta catctacaaa
     2881 acactacttg agacaatcat cgcaatatta gttaaatcgc ggtttttgat tagtttaatt
     2941 catgtgaata gttaagccag tcgccgcgtt ccctcttaca ctatgcgctg ttattagttc
     3001 gttactggaa gtccagtcac cttgtcagga gtattatcgt gattagtcta ttcgacatgt
     3061 ttaaggtggg gattggtccc tcatcttccc ataccgtagg gcctatgaag gcgggtaaac
     3121 agttcgtcga tgatctggtc gaaaaaggct tactggatag cgttactcgc gttgccgtgg
     3181 acgtttatgg ttcactgtcg ctgacgggta aaggccacca caccgatatc gccattatta
     3241 tgggtcttgc aggtaacgaa cctgccaccg tggatatcga cagtattccc ggttttattc
     3301 gcgacgtaaa agagcgcgaa cgtctgctgc tggcacaggg acggcatgaa gtggatttcc
     3361 cgcgcgacaa cgggatgcgt tttcataacg gcaacctgcc gctgcatgaa aacggtatgc
     3421 aaatccacgc ctataacggc gatgaagtcg tctacagcaa aacttattat tccatcggcg
     3481 gcggttttat cgtcgatgaa gaacactttg gtcaggatgc tgccaacgaa gtgagcgtgc
     3541 cgtatccgtt caaatctgcc accgaactgc tcgcgtactg taatgaaacc ggctattcgc
     3601 tgtctggtct cgctatgcag aacgaactgg cgctgcacag caagaaagag atcgacgagt
     3661 atttcgcgca tgtctggcaa accatgcagg catgtatcga tcgcgggatg aacaccgaag
     3721 gtgtactgcc aggcccgctg cgcgtgccac gtcgtgcgtc tgccctgcgc cggatgttag
     3781 tttccagcga taaactgtct aacgatccga tgaatgtcat tgactgggta aacatgtttg
     3841 cgctggcagt taacgaagaa aacgccgccg gtggtcgtgt ggtaactgcg ccaaccaacg
     3901 gtgcctgcgg tatcgttccg gcagtgctgg cttactatga ccactttatt gaatcggtca
     3961 gcccagacat ctatacccgt tactttatgg cagcgggcgc gattggtgca ttgtataaaa
     4021 tgaacgcctc tatttccggt gcggaagttg gttgccaggg cgaagtgggt gttgcctgtt
     4081 caatggctgc tgcgggtctt gcagaactgc tgggcggtag cccggaacag gtttgcgtgg
     4141 cggcggaaat tggcatggaa cacaaccttg gtttaacctg cgacccggtt gcagggcagg
     4201 ttcaggtgcc gtgcattgag cgtaatgcca ttgcctctgt gaaggcgatt aacgccgcac
     4261 ggatggctct gcgccgcacc agtgcaccgc gcgtctcgct ggataaggtc atcgaaacga
     4321 tgtacgaaac cggtaaggac atgaacgcca aataccgcga aacctcacgc ggtggtctgg
     4381 caatcaaagt ccagtgtgac taatacttct tactcgccca tctgcaacgg atgggcgaat
     4441 ttatacccgc tttctcgtct gctgtaatat tccccactac acttccactg ttgcgtcagg
     4501 cgtttgtcgc catacgctta cagggtggcc cgcatgcaaa aagcacaacg gatcattaaa
     4561 acctatcgcc gtaatcgaat gattgtttgt acgatttgcg cactcgttac gctcgcttcg
     4621 accctgagcg tgcgatttat ttcacagcgt aacttaaatc aacaacgggt agtacaattc
     4681 gccaatcacg ctgtagagga attagataaa gtactgcttc ccctacaggc aggtagcaaa
     4741 gtcttgcttc cgctgattgg tctgccctgc tctgtcgccc atttgccatt acgtaaacag
     4801 gcggcaaaac tccaaactgt gcgatccatt ggcctggtgc aagacggcac actttattgc
     4861 tccagcattt ttggttatcg caatgtgccc gtcgtggaca ttctggctga acttcctgca
     4921 ccgcaaccac ttttacgcct gacgatcgac cgtgccctga ttaaaggcag tccggttttg
     4981 attcaatgga cgcctgcagc gggcagtagc aatgctgggg tcatagagat gattaacatc
     5041 gacttactga cggcaatgct ccttgagcca caactgccgc aaatcagtag cgccagcctg
     5101 acggtggaca aacggcattt gctctatggt aatgggctgg tagattccct tccgcaacct
     5161 gaagacaatg aaaactatca ggtttcttcg caaagctttc cttttaccat taacgtaaat
     5221 ggtccggggg ctacggcgct ggcatggcac tatcttccaa cacaattacc gctggcggtg
     5281 ctgctaagtt tactggtggg ctatatcgcc tggctggcga ccgcttaccg gatgagcttt
     5341 tcccgcgaaa ttaatctggg cctggcgcaa catgagttcg aattgttctg tcagcctttg
     5401 cttaatgcgc gcagccagca atgtattggt gtagagattt tgctgcgctg gaataatccg
     5461 cgtcagggct ggatttcacc ggatgtgttt attcctatcg cggaagaaca tcatttaatt
     5521 gtgccactga cccactatgt gatggcagaa accattcgtc agcaccatgt tttcccgatg
     5581 agtagtcagt ttcatgttgg cattaacgtc gcacccagcc attttcgccg tggtgtgctg
     5641 ataaaagatc tcaatcagta ctggtttagc gctcacccga ttcagcaact gatcctcgaa
     5701 atcaccgaac gcgatgcctt actggatgtt gattatcgga ttgcccgcga gctgcatcgt
     5761 aaaaacgtca aactggcgat tgatgacttc ggcaccggca acagctcgtt ttcctggctt
     5821 gaaacattac gtcctgacgt gctgaaaatt gataagtcat ttaccgcagc tataggttct
     5881 gacgcggtta actcgacggt gaccgatatc atcatcgcgc tggggcaaag actgaatatt
     5941 gaactggtgg cggagggcgt ggaaacgcaa gaacaggcga agtatttgcg ccgtcatggc
     6001 gtgcatattt tgcaagggta tttgtacgca cagccgatgc cgctacgtga ttttcccaaa
     6061 tggctggcgg gcagccaacc gccgcccgcc cggcataatg gacatatcac gcccattatg
     6121 tcgttacgtt aacgcttact catcttcatc gtgcgccggt tgctctttaa caatgcgaac
     6181 cagatcaaca cgataatcat tggcttcaaa gatggtgata tgcagtggcc ctacatcaat
     6241 cacatcgccc acacggggaa tgtgaccatt tgccgagatc acgaggcccg cgaccgtcgc
     6301 gatatcatcg tcatcggcaa ggtgctcaac atcaagcgcc tgctgcaagg catgcaaatc
     6361 tgtaccgcct tttaccagcc agccgttacc atcggtaatg atttccggcg tttcgtcagc
     6421 gtccgggaat tcacccgcaa tggcttccag cacatccagc ggcgtgacca agccttgtac
     6481 cacaccaaac tcgttggtca cgataacaaa gctcccgcga gcgcgacgca gcacgcccag
     6541 caggttgatc ggatcgaggg tttccgggac gataatcgcc ggagacgccg aagcaatcgc
     6601 cgccacatca acgccctctt ccagcgccac cagcagttct ttagcacgta cgataccgat
     6661 gatttcatcc agttcaccgc gacataccgg gaacagactg tgcggtgaag agagcagttg
     6721 ctcgcggatt tcatcgaccc cgagattagc gtcaacccag cttatttcac cgcgcggcgt
     6781 catgatcccg cgcagagaac gcgacgccag cgtcagtacg ccgttaatca tgtaccgttc
     6841 ttcttcggca aatgcacctt ccgggatcgg catcgggtta tcggcatcgt gctgaacatt
     6901 ggcctgacgt ttcccgccca tcaaacgcag gatggcatcg gcagtacgcg ctcgcagcgg
     6961 caaagtcgac tggtgacgaa taaagttgcg acgcgcaatc tggttaaaca cttcgataat
     7021 gatcgagaag ccaatcgcgg catacaggta acctttcgga atgtggaaac cgaaaccttc
     7081 tgccaccaga ctcagaccaa tcattaacag gaagctcaga cagagcacca ccaccgtggg
     7141 gtgctggtta acgaatcgcg tcagcggttt cgatgccagc aacataaccg ccatcgcaat
     7201 cactaccgcc gccatcatca ccggcagatg gttaaccatc cctactgcag taattaccgc
     7261 atccaacgag aagacggcgt caaggatgac aatctgtgtg acgaccaccc agaaactggc
     7321 gtagccttta ccgtggccgg aatcatgatc gcggttttcc agccgttcat gcagttcggt
     7381 tgttgctttg aacagcaaga atatcccccc gaacaacata atcagatcgc gtccggagaa
     7441 ggagaaatcc atgacggtaa atagcggttt ggtcagcgtg accatccatg aaatcagcga
     7501 cagcagcccc agacgcataa tcagcgccag tgataacccc agcaaacgcg ctttatcgcg
     7561 ttgttttggc ggcagtttgt cagcaagaat ggcgatgaag accaggttat cgatacccag
     7621 cacaatttcg agaacaacaa gcgtgagtag ccccgcccaa attgaggggt ccattaagaa
     7681 ttccatgaca agctcctgct taaggaatag ctattcgacg ccagaaataa tgcaggcgta
     7741 acgacaaaat gcaaacgaaa ggtgcggcat agagtgccag aaaggcaggc gttaaaaggc
     7801 ctgatgctga aatgacgtcg gtgacgatcc atactgcggg ctactgccct atactccatg
     7861 gttgttaaac gggagttaaa catatcagag acgcctctga tttggcaaag atttaccttc
     7921 ctttgcaaac gaatgtgaca aggatatttt acctttcgaa atttctgata atcgaaagtt
     7981 aaattacgga tcttcatcac ataaaataat tttttcgata tctaaaataa atcgcgaaac
     8041 gcaggggttt ttggttgtag cccttatctg aatcgattcg attgtggacg acgattcaaa
     8101 aatacatctg gcacgttgag gtgttaacga taataaagga ggtagcaagt gaccattgct
     8161 attgttatag gcacacatgg ttgggctgca gagcagttgc ttaaaacggc agaaatgctg
     8221 ttaggcgagc aggaaaacgt cggctggatc gatttcgttc caggtgaaaa tgccgaaacg
     8281 ctgattgaaa aatacaacgc tcagttggca aaactcgaca ccactaaagg cgtgctgttt
     8341 ctcgttgata catggggagg cagcccgttc aatgctgcca gccgcattgt cgtcgacaaa
     8401 gagcattatg aagtcattgc aggcgttaac attccaatgc tcgtggaaac gttaatggcc
     8461 cgtgatgatg acccgagctt tgatgaactg gttgcgctgg cagtagaaac aggccgtgaa
     8521 ggcgtgaaag cactgaaagc caaaccggtt gaaaaagccg cgccagcacc cgctgccgca
     8581 gcaccaaaag cggctccaac tccggcaaaa ccaatggggc caaacgacta catggttatt
     8641 ggccttgcgc gtatcgacga ccgtctgatt cacggtcagg tcgccacccg ctggaccaaa
     8701 gaaaccaatg tctcccgtat tattgttgtt agtgatgaag tggctgcgga taccgttcgt
     8761 aagacactgc tcacccaggt tgcacctccg ggcgtaacag cacacgtagt tgatgttgcc
     8821 aaaatgattc gcgtctacaa caacccgaaa tatgctggcg aacgcgtaat gctgttattt
     8881 accaacccaa cagatgtaga gcgtctcgtt gaaggcggcg tgaaaatcac ctctgttaac
     8941 gtcggtggta tggcattccg tcagggtaaa acccaggtga ataacgcggt ttcggttgat
     9001 gaaaaagata tcgaggcgtt caaaaaactg aatgcacgcg gtattgagct ggaagtccgt
     9061 aaggtttcca ccgatccgaa actgaaaatg atggatctga tcagcaaaat cgataagtaa
     9121 cgtattgtgt tgattatcat tcagttttca cacttaagtc ttacgtaaac aggagaagta
     9181 caatggagat taccactctt caaattgtgc tggtatttat cgtagcctgt atcgcaggta
     9241 tgggatcaat cctcgatgaa tttcagtttc accgtccgct aatcgcgtgt accctggtgg
     9301 gtatcgttct tggggatatg aaaaccggta ttattatcgg tggtacgctg gaaatgatcg
     9361 cgctgggctg gatgaacatc ggtgctgcag ttgcgcctga cgccgctctg gcttctatca
     9421 tttctaccat tctggttatc gcaggtcatc agagcattgg tgcaggtatc gcactggcaa
     9481 tccctctggc cgctgcgggc caggtactga ccatcatcgt tcgtactatt accgttgctt
     9541 tccagcacgc tgcggataag gctgctgata acggcaacct gacagcgatt tcctggatcc
     9601 acgtttcttc tctgttcctg caagcaatgc gtgtggctat tccggccgtc atcgttgcgc
     9661 tgtctgttgg taccagcgaa gtacagaaca tgctgaatgc gattccggaa gtggtgacca
     9721 atggtctgaa tatcgccggg ggcatgatcg tggtggttgg ttatgcgatg gttatcaaca
     9781 tgatgcgtgc tggctacctg atgccgttct tctacctcgg cttcgtaacc gcagcattca
     9841 ccaactttaa cctggttgct ctgggtgtga ttggtactgt tatggcagtg ctctacatcc
     9901 aacttagccc gaaatacaac cgcgtagccg gtgcgcctgc tcaggcagct ggtaacaacg
     9961 atctcgataa cgaactggac taacaggtga gcgaaatggt tgatacaact caaactacca
    10021 ccgagaaaaa actcactcaa agtgatattc gtggcgtatt cctgcgttct aacctcttcc
    10081 agggttcatg gaacttcgaa cgtatgcagg cactgggttt ctgcttctct atggtaccgg
    10141 caattcgtcg cctctaccct gagaacaacg aagctcgtaa acaagctatt cgccgtcacc
    10201 tggagttctt taacacccag ccgttcgtgg ctgcaccgat tctcggcgta accctggcgc
    10261 tggaagaaca gcgtgctaat ggcgcagaga tcgatgacgg tgctatcaac ggtatcaaag
    10321 tcggtttgat ggggccgctg gctggtgtag gcgacccgat cttctgggga accgtacgtc
    10381 cggtatttgc agcactgggt gccggtatcg cgatgagcgg cagcctgtta ggtccgctgc
    10441 tgttcttcat cctgtttaac ctggtgcgtc tggcaacccg ttactacggc gtagcgtatg
    10501 gttactccaa aggtatcgat atcgttaaag atatgggtgg tggcttcctg caaaaactga
    10561 cggaaggggc gtctatcctc ggcctgtttg tcatgggggc attggttaac aagtggacac
    10621 atgtcaacat cccgctggtg gtttcgacca tcaccgggca ggatggacag acccgtgtca
    10681 ccaccgtgca aactattctt gaccagttaa tgccaggcct tgtaccactg ctgctgacat
    10741 ttgcttgtat gtggctgctg cgcaaaaaag ttaacccgct gtggatcatc gttggcttct
    10801 tcgtcatcgg tatcgctggt tacgcttgcg gcctgctggg actgtaagac tgtcgtacac
    10861 taccggggcc ttttggcccc gtttttttat ctggaggatt aatgacaatc acggacctgg
    10921 tactgattct tttcatcgcc gcactcctgg ccttcgcgat ctacgatcag ttcatcatgc
    10981 cccgccgtaa cggccccacc ctgctggcaa ttcctttgct ccggcgtggt cgcatcgata
    11041 gcgttatctt cgtcggattg attgtcattc ttatctataa caacgtcacg aatcatggcg
    11101 cgctaataac aacatggtta ttaagtgcac tggctctgat gggtttttat atattctgga
    11161 tccgcattcc gaagatcatc tttaaacaaa aaggtttttt cttcgccaat gtctggattg
    11221 aatatagccg aatcaaagcg atgaacttgt cggaagatgg cgtgttggtg atgcaattag
    11281 aacagcgtcg gctgttaatc cgcgttcgaa atatcgacga tctggaaaaa atttataagc
    11341 ttctc
//
LOCUS       DAZPEU010000122        11282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPEU010000122 DAZPEU010000000
VERSION     DAZPEU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.123"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(201..1865)
                     /gene="glnS"
                     /locus_tag="ACHU2L_002847"
     CDS             complement(201..1865)
                     /gene="glnS"
                     /locus_tag="ACHU2L_002847"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR1357626.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2068..4014)
                     /gene="nagE"
                     /locus_tag="ACHU2L_002848"
     CDS             complement(2068..4014)
                     /gene="nagE"
                     /locus_tag="ACHU2L_002848"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR1357627.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4347..5147
                     /gene="nagB"
                     /locus_tag="ACHU2L_002849"
     CDS             4347..5147
                     /gene="nagB"
                     /locus_tag="ACHU2L_002849"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1357628.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5207..6355
                     /gene="nagA"
                     /locus_tag="ACHU2L_002850"
     CDS             5207..6355
                     /gene="nagA"
                     /locus_tag="ACHU2L_002850"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1357629.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6364..7584
                     /gene="nagC"
                     /locus_tag="ACHU2L_002851"
                     /gene_synonym="nagR"
     CDS             6364..7584
                     /gene="nagC"
                     /locus_tag="ACHU2L_002851"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR1357630.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7632..8384
                     /gene="nagD"
                     /locus_tag="ACHU2L_002852"
                     /pseudo
     CDS             7632..8384
                     /gene="nagD"
                     /locus_tag="ACHU2L_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(8435..8533)
                     /locus_tag="ACHU2L_002853"
                     /pseudo
     CDS             complement(8435..8533)
                     /locus_tag="ACHU2L_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8781..10445
                     /gene="asnB"
                     /locus_tag="ACHU2L_002854"
     CDS             8781..10445
                     /gene="asnB"
                     /locus_tag="ACHU2L_002854"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR1357631.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10824..10900
                     /locus_tag="ACHU2L_002855"
     tRNA            10824..10900
                     /locus_tag="ACHU2L_002855"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10858..10860,aa:Met,seq:cat)
     gene            10909..10993
                     /locus_tag="ACHU2L_002856"
     tRNA            10909..10993
                     /locus_tag="ACHU2L_002856"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10943..10945,aa:Leu,seq:tag)
     gene            11017..11091
                     /locus_tag="ACHU2L_002857"
     tRNA            11017..11091
                     /locus_tag="ACHU2L_002857"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11049..11051,aa:Gln,seq:ttg)
     gene            11107..11183
                     /locus_tag="ACHU2L_002858"
     tRNA            11107..11183
                     /locus_tag="ACHU2L_002858"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11141..11143,aa:Met,seq:cat)
BASE COUNT         2811 a         2904 c         2853 g         2714 t
ORIGIN      
        1 tttgggattg tttgctattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa
       61 agcacgcaaa ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg
      121 caatttagcg tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc
      181 cggcttggta gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg
      241 gtgcggttaa ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct
      301 tcacgctcaa actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag
      361 ccctgtttga tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct
      421 gggttaggca cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc
      481 gcgctcaccc agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag
      541 gtgtcggcgt cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg
      601 cgttcagcct taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt
      661 ttgtactgct tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta
      721 aacggcacct gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt
      781 tcgccttcgc cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg
      841 cgcggcgcat tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg
      901 gtgttgtcct gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg
      961 taaccacgac gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg
     1021 tgcttgtcgg tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc
     1081 aggcgcgaga attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag
     1141 tcgtacagac gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc
     1201 agcgcatcgc tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca
     1261 gtctggtggt gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat
     1321 ggcgacgcca tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca
     1381 cgcatttttt cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt
     1441 ttacccggct gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca
     1501 acgtacgcca ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa
     1561 tcggaggagt aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg
     1621 atcgaatcga catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg
     1681 cactggcctt tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca
     1741 atatgcagat agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca
     1801 ctggccagat cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca
     1861 ctcatcgtgg attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg
     1921 acaggctgac aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat
     1981 tgatgaaaaa aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct
     2041 gccgtgtcgc atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc
     2101 cgccacaaca tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca
     2161 aaccaccggg ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag
     2221 aatcggttgc cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc
     2281 ttccagcgct acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc
     2341 caggcagaac gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac
     2401 gacgatttta tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc
     2461 ttcgtcagga acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc
     2521 gatagatacc gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt
     2581 tgcttcagct gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc
     2641 gatggattct gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac
     2701 accagaagca cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt
     2761 caggcgcaga cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc
     2821 agcaatatag ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc
     2881 agtaacgatc tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat
     2941 aaccaaactg aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat
     3001 ccagacgttc tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc
     3061 agagaaagag aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt
     3121 cagcagtgcg tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg
     3181 ctcagtcaca ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat
     3241 cggacgacgc tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa
     3301 catcatgatc gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa
     3361 acggttgatg tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa
     3421 ccaggcgatg gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa
     3481 accaaagata ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat
     3541 agcgtgctgt accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa
     3601 gccggtggcg atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt
     3661 aatatcggac caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag
     3721 tacacccatg ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc
     3781 taccgcaccc gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc
     3841 gatggcgaag attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc
     3901 aacgttaagt aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc
     3961 gatagggagc tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt
     4021 ccccctacga gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt
     4081 tactgccgat gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt
     4141 aattcgcatc gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc
     4201 atcactccct tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg
     4261 gattgacgcc tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc
     4321 ttacatttta cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg
     4381 tcggcaaatg ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg
     4441 atcgtccgtt tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt
     4501 tagtcgaaat gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg
     4561 acgaatatgt cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta
     4621 atttcttcga tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc
     4681 cggatatcga tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc
     4741 atctgtttat gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt
     4801 ctctggcttc tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc
     4861 gtttctttga taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta
     4921 cactgctgga tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc
     4981 tgcaggccgc cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc
     5041 atccgaaagc gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt
     5101 taagatattt caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc
     5161 cctggttcct tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac
     5221 ccagggccgg atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga
     5281 tggcctgatt aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc
     5341 actgaacggg gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg
     5401 cgtacagttt aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa
     5461 agccaatgag aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga
     5521 gctgatgaaa cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc
     5581 gttaggtctg catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc
     5641 gaattttgtg cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt
     5701 cattaccaaa gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc
     5761 aaatgccggg attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc
     5821 cggtttccgc gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac
     5881 cggtcgtgaa ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat
     5941 tatcgctgat ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg
     6001 cgacaaactg tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt
     6061 catttttgcg ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac
     6121 gttaagcggt tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg
     6181 tatcgcactg gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt
     6241 tgaaaaacgt ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc
     6301 tgattttaaa atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa
     6361 agtatgacac caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac
     6421 agcgcggcgg tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc
     6481 gagcaaagcc agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc
     6541 gggctgatca aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc
     6601 gtcaccgaaa cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc
     6661 atcactctgt ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa
     6721 cgtacccagc agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc
     6781 taccagcgca agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac
     6841 ccggacagcg gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta
     6901 gaagcactgg aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg
     6961 gcgctggcgg agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt
     7021 gtccatcgcg gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac
     7081 ggtaacgtcg gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc
     7141 ggcaactttg gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg
     7201 aatctgttaa agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact
     7261 atctgcaaag ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt
     7321 cgtcatctgg gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt
     7381 attgctggtg aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt
     7441 aatacccaag cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat
     7501 caccgctcgg caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg
     7561 ctccagcatt tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt
     7621 ctgggtagtc catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg
     7681 ataacgtcgc cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc
     7741 cgctggtgtt gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg
     7801 ccactgccgg tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg
     7861 atttcctgcg tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc
     7921 atgaactgta caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg
     7981 gcgaaacacg ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg
     8041 gtgcacgctt tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg
     8101 gcgcgttgtg tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc
     8161 ccagcccgtg gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag
     8221 tgattgtcgg cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga
     8281 cgattctggt gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc
     8341 caagctggat ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg
     8401 cggacgtggt tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc
     8461 ctacgcagct cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca
     8521 cgccgcatcc ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa
     8581 ccattcaata aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga
     8641 ccctttttca ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct
     8701 tgcgcacagt gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata
     8761 ccaggttaac ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg
     8821 cagttgagct gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg
     8881 actggtccgg tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg
     8941 ttgacgttaa cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg
     9001 taaacggtga aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt
     9061 tccagacagg gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat
     9121 ttctcgacga cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct
     9181 acctgattgg tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg
     9241 gtcagctgta tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag
     9301 agttcccggc ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc
     9361 gcgactggtt cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc
     9421 aggcactgga agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac
     9481 tttctggtgg tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc
     9541 gcgtggaaga tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag
     9601 gtctgccggg ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg
     9661 tgcatcacga aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt
     9721 accacatcga aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt
     9781 cgcgtaagat caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag
     9841 tgtttggcgg ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga
     9901 cggtgcgtaa actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt
     9961 cagcctgggg cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga
    10021 tgcgcattaa cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc
    10081 gtgaatgttt tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct
    10141 ccgatggcgt cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt
    10201 ctgatcagca actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag
    10261 aagcgtatct gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt
    10321 gcgtgccggg cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag
    10381 cgttcaaaaa aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata
    10441 agtaagaaat gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt
    10501 gttgattctg atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact
    10561 catcactgta ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat
    10621 ttcatcctgt acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa
    10681 ttgacgaata tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt
    10741 tccttgaaaa agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg
    10801 ataaggtatc gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat
    10861 aatgatgggg tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg
    10921 aaattggtag acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc
    10981 gcctcccgca ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc
    11041 accggttttt gataccggca ttccctggtt cgaatccagg taccccagcc atcgaagaaa
    11101 taatctggct acgtagctca gttggttaga gcacatcact cataatgatg gggtcacagg
    11161 ttcgaatccc gtcgtagcca ccaaattctg aatgtgtcga atacgttcgg caaattcaaa
    11221 aaccaatttg ttggggtatc gccaagcggt aaggcaccgg attctgattc cggcattccg
    11281 ag
//
LOCUS       DAZPEU010000123        11248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPEU010000123 DAZPEU010000000
VERSION     DAZPEU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.124"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..612)
                     /gene="hdhA"
                     /locus_tag="ACHU2L_002859"
     CDS             complement(<1..612)
                     /gene="hdhA"
                     /locus_tag="ACHU2L_002859"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR1357632.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITPE"
     gene            complement(724..1752)
                     /gene="malI"
                     /locus_tag="ACHU2L_002860"
     CDS             complement(724..1752)
                     /gene="malI"
                     /locus_tag="ACHU2L_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR1357633.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1927..3519
                     /gene="malX"
                     /locus_tag="ACHU2L_002861"
     CDS             1927..3519
                     /gene="malX"
                     /locus_tag="ACHU2L_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR1357634.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3529..4701
                     /gene="malY"
                     /locus_tag="ACHU2L_002862"
     CDS             3529..4701
                     /gene="malY"
                     /locus_tag="ACHU2L_002862"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR1357635.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4805..5806
                     /gene="add"
                     /locus_tag="ACHU2L_002863"
     CDS             4805..5806
                     /gene="add"
                     /locus_tag="ACHU2L_002863"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR1357636.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5842..6882)
                     /locus_tag="ACHU2L_002864"
     CDS             complement(5842..6882)
                     /locus_tag="ACHU2L_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1357637.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7125..7250
                     /gene="blr"
                     /locus_tag="ACHU2L_002865"
     CDS             7125..7250
                     /gene="blr"
                     /locus_tag="ACHU2L_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR1357638.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7523..7738
                     /gene="ydgT"
                     /locus_tag="ACHU2L_002866"
     CDS             7523..7738
                     /gene="ydgT"
                     /locus_tag="ACHU2L_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR1357639.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7824..8264
                     /locus_tag="ACHU2L_002867"
     CDS             7824..8264
                     /locus_tag="ACHU2L_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR1357640.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8341..8922
                     /gene="rsxA"
                     /locus_tag="ACHU2L_002868"
     CDS             8341..8922
                     /gene="rsxA"
                     /locus_tag="ACHU2L_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR1357641.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8922..9500
                     /gene="rsxB"
                     /locus_tag="ACHU2L_002869"
     CDS             8922..9500
                     /gene="rsxB"
                     /locus_tag="ACHU2L_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR1357642.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9493..>11248
                     /gene="rsxC"
                     /locus_tag="ACHU2L_002870"
     CDS             9493..>11248
                     /gene="rsxC"
                     /locus_tag="ACHU2L_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR1357643.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVD"
BASE COUNT         2620 a         2894 c         2834 g         2900 t
ORIGIN      
        1 ttctggtgta ataacggatt tcagggcatc ggttaatatt gcccccggcg caatgctatt
       61 cacccgaata tttttttcac ccaggtcaaa tgccatattt ctgaccagat gactggccgc
      121 agctttagat gatgcatagg aagtcatgtt tatattttta ttttctgccg ccatagaagt
      181 gatggtcaga ataacgccac ctccattttt ttccatttct ggcgcaacaa gttgtgacag
      241 atggaaaaaa gaaaacacat tcagttcata agcacggcga aaatctgcca ttggcatatc
      301 aaacggttta ggaccaccgc caccggcgtt gttaaccagg atatcaactt tacccagatt
      361 actgacggca aagtctgcca gtgcagagag ttcctgttcg gaagtaatat cacaacgcca
      421 ggcaaatgcc tgaccatcca gttgttgaat ttcgtctaca acatggttag ctgcgtcggc
      481 gttaatatca ctgaccacca cagatgcgcc agctgtcgcg aatgtaatgg cgatttcttt
      541 accaatacct gcacccgcac ctgtgatgat ggcgcatttt ccgtcgagtc tcaggttgtc
      601 agaattaaac acataaacct cctgtgaact tcagtaaaaa taaaaggcac cgcctgtgtg
      661 ccacattttt tatcattaac cttgaggaaa ggacactacc aagtatagca gataaagaga
      721 ggattatttc gccgcaatga gccgggcggg aataataaga ttgcgtgaat gcgtctcttc
      781 atgggtgatt ttttgcatca tgcgatccgc aagtgtgata ccaagttccc gcgctggcgt
      841 gctggcccag gtaacaggta tatcatcaag tgtggttggt gtcgcatcgg taaatgccgc
      901 cagcgaaacc tgttgctcaa agtaacgatc gactccgctt tccccgcttt gccgccctgc
      961 tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg ttatagcaaa ccacggcact
     1021 gatggtcggg ttatgacgta aaagcgccgt gatagcttcc gcggcttgct tctggctgga
     1081 agtgcactcc aacacccaat cgctgtgaaa cggcaggcca aattttagta gagttgcaca
     1141 atagcccccc acccgttctg cacgggttaa tgaggaactt tgccctccca gccaggcgat
     1201 ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac tgtgcagcct gcatgttgtc
     1261 cgggcgaacc gtatcaacat catcaagata actggcacgg gaagcgaaaa tcaccgggat
     1321 agctttttct tctgccatcc gccgcaggtc atcgctgctt cctgcagccc cggcaattac
     1381 cacaccatcg acaccctgat tcagtaacag tgaaaaccgc tgtgccagct gctcgccgtc
     1441 tttaccgccg tgaagcaaaa aaaccatccg tccctgtgct tccagagctt ccgtcaatcc
     1501 ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg acgatcaaac caatgacgcc
     1561 gctttgcccg ccgcgcagcg ccgacgcctg gcgattgcgc acaaatccca gctcttcaat
     1621 ggcggcgtta acgcgttctc ctgtggcggt agagattcgc cctttgccat taagcaccag
     1681 cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca tcatgaatgg ttattttttt
     1741 ggcggtagcc atgcagatga cctactccct gattatgtga cagataaaac gttttacctt
     1801 ttatttatct tatacccgct attatcgttg cgtaatgtga tttatgactc actaaaattt
     1861 gataaaacgt tttatcttct cgcgcaattt actgaatcca gattgttctc tacgaggagt
     1921 cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt gggagttctt ccagcagtta
     1981 ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct gcggcattat gctcggcatt
     2041 ggtagttctc ttagcagcca tgatgtcata accctgatcc cggtcctggg caaccccgtg
     2101 ttgcaggcta tctttacctg gatgagtaag attggctcgt ttgcttttag tttcctgcct
     2161 gtcatgttct gtatcgccat cccgctgggc ctggcacgcg aaaataaagg cgtagcggca
     2221 ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg taaacttctg gttgaccaat
     2281 aaaggcattc tgccaaccac agatgccgcc gttctgaaag ccaataacat ccagagcatt
     2341 cttgggatcc agtcgatcga taccgggatc ctcggtgcgg tgatcgccgg tattatcgtc
     2401 tggatgctgc atgagcgttt ccataatatc cgcctgccgg atgcgctggc attcttcggc
     2461 ggtacgcgct tcgtaccaat tatctcctcg ctggtgatgg gccttgtcgg cctggtgatt
     2521 ccattagtct ggccgatttt cgccatgggt attagcggct tgggccatat gataaacagc
     2581 gcgggtgatt tcggaccgat gctgtttggt accggtgaac gtctgctgtt gccgtttggt
     2641 ctgcatcaca ttctggtggc attaattcgc tttaccgacg caggcggcac gcaggaggtc
     2701 tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg cgcaattgag ttgcccgacc
     2761 actcacggtt tttctgaaag cgtcacgcgt ttcctttcgc aaggtaaaat gcctgcgttt
     2821 ctcggcggtc taccaggtgc agcgttagct atgtatcact gcgcgcgccc ggaaaatcgc
     2881 cataaaatta aaggtctgct gatttctggc ctgatcgcct gcgtcgttgg cggcactacc
     2941 gaaccgctgg aattcctgtt cctgttcgta gcgccagttc tgtatgtcat ccacgcgctg
     3001 ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg tcaccatcgg taataccgac
     3061 ggcaatatca tcgacttcgt ggtgttcggt attttgcatg gtctgtcaac caagtggtac
     3121 atggtgccag tggtggcggc aatctggttt gtcgtttact acgtcatctt ccgtttcgct
     3181 atcacccgct tcaatctgaa aaccccgggg cgcgatagcg aagttgccag ctcaatcgaa
     3241 aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg ttcctgcaat cctcgaagca
     3301 ttaggcggtg ccgacaatat tgtcagcctc gataactgca ttacccgtct gcgtttgtct
     3361 gtggaagata tgtcgcttgt taatgtgcag gcactgaagg acaatcgggc aattggcgta
     3421 gtacaactta atcaacataa cctgcaggtt gttatcgggc cacaagttca gtcagtaaaa
     3481 gatgaaatgg ccggtctgat gcatactgtc caggcataag gataagatat gttcgatttt
     3541 tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt gggattatgt cgctgaccgt
     3601 ttcggcactg ctgacctgtt accgttcacg atttcagaca tggattttgc cactgccccc
     3661 tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg tatttggcta cagccgctgg
     3721 aaaaacgatg agtttctcgc ggctattgcc cactggtttt ccacccagca ttacaccgca
     3781 attgatacgc agacggtggt gtatggccct tctgtcatct atatggtttc agaactgatt
     3841 cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca cgcccgccta tgacgcattt
     3901 tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg ttgctttaga gaagcaggct
     3961 gacggctggt tttgcgatat gggcaagctg gaagccgttt tggcgaaacc agaatgtaaa
     4021 attatgctcc tgtgtaaccc acagaatcct accgggaaag tgtggacgtg cgatgagctg
     4081 gagatcatgg ctgacctgtg cgagcgtcat gatgtgcggg ttatttccga tgaaatccat
     4141 atggatatgg tctggggcga gcagccgcat attccctgga gtaatgtggc gcggggagac
     4201 tgggcgttgc tgacatcggg atcgaaaagt ttcaatattc ccgcgctgac cggtgcttac
     4261 gggattatag aaaatagcag cagccgtgat gcctatttat cggcactgaa aggacgtgat
     4321 gggctttctt ccccttcggt actggcgtta actgcccata tcgccgccta tcagcaaggt
     4381 gcaccgtggc tggatgcctt acgcgtctat ctgaaagata acctgacgta tatctcagat
     4441 aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc cgcaatccac ttatctggcc
     4501 tggcttgatc tacgtccgtt gaatattgac gacaacgcgt tgcaaaaagc gcttatcgag
     4561 caagaaaaag tcgcgatcat gccggggtat acctatggtg aagaaggtcg tggttttgtc
     4621 cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag gtgtggctgg attaataaac
     4681 gccatccgcg ctgttcgcta accccaattg cgcaacgtaa aaaatcgttg cgcaatcgtg
     4741 gatttttacc ctgctttgtt tttataatgg tgcgcacttt tatatccaga aaaagagtgc
     4801 gaccatgatt gataccaccc tgccattaac tgatatccat cgccaccttg atggcaacat
     4861 tcgtccccag accattcttg aacttggccg ccagtataat atctcgcttc ctgcacaatc
     4921 ccttgaaaca ctgattcccc acgttcaggt cattgccaac gaacccgatc tggtgagctt
     4981 tctgaccaaa cttgactggg gcgttaaagt tctcgcctct cttgatgcct gccgccgcgt
     5041 ggcatttgaa aacattgaag atgcagcccg taacggcctg cgctatgtcg agctgcgttt
     5101 ttcaccaggc tacatggcaa tggcacataa gctgcctgta gcgggtgttg tcgaagcggt
     5161 gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg caggcgaagc ttatcggcat
     5221 tatgagccga accttcggcg aagccgcctg tcagcaagag ctggaggcct ttttagccca
     5281 ccgtgaccag attaccgcac ttgatttagc tggtgatgaa cttggattcc cgggaagtct
     5341 gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg catattaccg tccatgcagg
     5401 cgaagctgcc gggccggaaa gcatctggca ggcgattcgt gaactgggtg cggagcgtat
     5461 tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg gattttctcg ccgagcaaca
     5521 aattggtatt gaatcctgtc tgacctccaa tattcagacc agcaccgtag cagagctggc
     5581 tgcacatccg ctgaaaacgt tccttgagca tggcattcgt gccagcatta acactgacga
     5641 tcccggcgta cagggagtgg atatcattca cgaatatacc gttgccgcgc cagctgctgg
     5701 gttatcccgt gagcaaatcc gccaggcaca gattaatggt ctggaaatgg ctttcctcag
     5761 cgcagaggaa aaacgcgcac tgcgagaaaa agtcgccgcg aagtaacaaa aatggatggt
     5821 gcaaattgca ccatccattt ttcacgcaag gcacaacgtc gcgcgatgtt tggcggattc
     5881 gatgcccagc tcaatcaact ccattacctg gattgcctgg cttgccggaa ccggattttc
     5941 accatcgcca tttaacgcat cacgaatcgc cgcatagtaa gccggataat tcccaggcac
     6001 cgtcaacagc gtttcttcga cacgttcctc accttccacg cgggtaagta cgccatcacg
     6061 catatcgtag ccccagtctt cctgcggcag acgctcgcca tttttcagac gttcttcctg
     6121 tggatcgagg ccatatttca cataactgcc tcgggatcca tgcacgatat accgtgctga
     6181 ctcagcggct gccagcatgg taccgtgtaa aatgactcgc cgctgtggat aggacaagat
     6241 ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac tgtgccaaat ctaccgtcat
     6301 gctgaccggt aaaccaaata gcgtaatggc ctgatcaaga agatgcggtg ctaaatcgta
     6361 ccagataccg ctgcctgggc cgccctgttc acgccaacga tcgcgcacct gcggacggaa
     6421 gcggtcaaaa tgagactcaa agtaagcaac ttcacccagc acgccttccg cgagcaaacc
     6481 ttttagcgtc aagaaatcgc tatcccaacg gcggttatgg aatacagaca gcacacgccc
     6541 caggcttttt gccagcgcat ccagctctcg cgcttgtgac agtgtcacgg taaagggttt
     6601 atcaacgacc acatgtttac ccgcctcaag cgccgctttg gctaacggga aatgggtatc
     6661 gttgggtgta ggaatgacaa tcaggtctat gttgggatcg ttaaacagat gcttcggctc
     6721 agagacaacc gtaaccgttg gccagtcggc ttttactttt gtttcatcac tgctggagat
     6781 taccgccagt tccagcccgg gcgtgcccgc aatcaggggc gcatggaagg ttttgctcgc
     6841 ataaccatac ccaatcaacc caacacggat gttgtcgctc atgtcattgc ctctcattga
     6901 agtatgatgg ctatttgaca ctatccttta cccacgctca acagtttaat aacctgccag
     6961 caataaggga tgttgtttaa cttaagtcaa aaaaatagcg aattttccaa cgacaaaagc
     7021 taaatatcgc aaaaacctca gtaaaaatct tgctggagct attattgcta agtaacattt
     7081 accccctgaa gttaatggat caatcaagag agatgtgggc tgtaatgaat cgtcttattg
     7141 aattaacagg ttggatcgtt cttgtcgttt cagtcattct tcttggcgtg gcgagtcaca
     7201 ttgacaacta tcagccacct gaacagagtg cttcggtaca acacaagtaa gctctgcact
     7261 tgtggagcga catgctgccc gtccgggtgc atgttttcac ttgtcggata ttaaaccagg
     7321 aatttattat cttgttcgat gttgttggtg attgtcaggg atagtaaagc gcgttactct
     7381 tttcagagca gtttttcatc cctgaatatt tgccatcagc gcattcttat tattgtaata
     7441 acacaagcgt tcacttttgg tgaaatttta tcatcactaa taatacctac ccctttggta
     7501 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     7561 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     7621 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7681 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7741 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7801 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7861 tgcttggccc gttggcctgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7921 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7981 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     8041 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     8101 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagttg
     8161 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     8221 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     8281 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     8341 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     8401 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     8461 atggggctgg caacaacgtt tgtgatgacg ctgacgtcta tttgcgcctg gcttatcgat
     8521 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     8581 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     8641 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8701 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8761 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8821 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8881 tctctggcct ttatgggctt tagtggtttg gtgaagttgt aatgaatgct atctggattg
     8941 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
     9001 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     9061 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     9121 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     9181 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     9241 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     9301 gtccggtaga cgccatcgtt ggcgctaccc gtgtcatgca tacggtaatg agtgatctct
     9361 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     9421 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     9481 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     9541 aacggcggca tccatccacc ggagatgaaa acccagacca acggtacccc cctgcgccag
     9601 gtactactgg cgcagcattt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9661 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9721 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9781 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9841 tgctggatcc cgcgcgacgg ctgggtcgat tatcgctctc gcagtcgcga agagttaatc
     9901 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg ccggattccc gacaggcgtt
     9961 aaattgcagg gtggtggaga taagattgaa acgttgatta tcaacgctgc tgagtgcgag
    10021 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
    10081 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
    10141 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
    10201 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
    10261 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
    10321 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
    10381 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
    10441 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
    10501 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
    10561 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
    10621 tgcatccggt gtagcgcctg tgctgacgcc tgccctgtgg atcttttgcc gcaacagttg
    10681 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgccgactgc
    10741 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccttggt gcaatatttc
    10801 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10861 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcgac tcgccttgaa
    10921 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10981 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    11041 gaacgcccgg ataacagtgc aattattgca gaacgggaag cccgtaaagc gcaagccaga
    11101 gcgaaacagg cagaactgga gcaaactaac gacgcagcaa ccgttgctga accacgtaaa
    11161 actgccgttg aagcagctat cgcccgctcc aaagcgcgca agctggaaca gcaacaggct
    11221 aatgcggaac cagaagaaca ggtcgatc
//
LOCUS       DAZPEU010000124        11206 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPEU010000124 DAZPEU010000000
VERSION     DAZPEU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.125"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            189..2894
                     /gene="malT"
                     /locus_tag="ACHU2L_002871"
                     /pseudo
     CDS             189..2894
                     /gene="malT"
                     /locus_tag="ACHU2L_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(2937..3953)
                     /gene="rtcA"
                     /locus_tag="ACHU2L_002872"
     CDS             complement(2937..3953)
                     /gene="rtcA"
                     /locus_tag="ACHU2L_002872"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR1357644.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(3957..5183)
                     /gene="rtcB"
                     /locus_tag="ACHU2L_002873"
     CDS             complement(3957..5183)
                     /gene="rtcB"
                     /locus_tag="ACHU2L_002873"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR1357645.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5364..6962
                     /gene="rtcR"
                     /locus_tag="ACHU2L_002874"
     CDS             5364..6962
                     /gene="rtcR"
                     /locus_tag="ACHU2L_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR1357646.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(6944..7702)
                     /locus_tag="ACHU2L_002875"
     CDS             complement(6944..7702)
                     /locus_tag="ACHU2L_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR1357647.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7719..8549)
                     /gene="glpG"
                     /locus_tag="ACHU2L_002876"
     CDS             complement(7719..8549)
                     /gene="glpG"
                     /locus_tag="ACHU2L_002876"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR1357648.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8594..8920)
                     /gene="glpE"
                     /locus_tag="ACHU2L_002877"
     CDS             complement(8594..8920)
                     /gene="glpE"
                     /locus_tag="ACHU2L_002877"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR1357649.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9110..10615
                     /gene="glpD"
                     /locus_tag="ACHU2L_002878"
     CDS             9110..10615
                     /gene="glpD"
                     /locus_tag="ACHU2L_002878"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR1357650.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANALMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10821..11102)
                     /locus_tag="ACHU2L_002879"
     CDS             complement(10821..11102)
                     /locus_tag="ACHU2L_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357651.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         2694 a         3079 c         2874 g         2559 t
ORIGIN      
        1 acgctggatc agcgtcgttt taggtgagtt gttaataaag atttggaatt gtgacacagt
       61 gcaaattcag acacataaaa aaacgtcatc gcttgcatta gaaaggtttc tggccgacct
      121 tataaccatt aattacgaag cgcaaaaaaa ataatatttc ctcattttcc acagtgaagt
      181 gattaactat gctgattccg tcaaaactaa gtcgtccggt tcgactcgac cataccgtgg
      241 ttcgtgagcg cctgctggct aaactttccg gcgcgaacaa cttccggctg gcgctgatca
      301 cgagtcctgc gggttacgga aagaccaccc tcatttccca gtgggcggca ggcaaaaacg
      361 atatcggctg gtactcgctg gatgaaggtg ataaccagca agagcgtttc gccagctatc
      421 tcattgccgc cgtgcagcag gcaaccaacg gtcactgtgc gatatgtgag acgatggagc
      481 aaaaacggca atatgccagc ctgacgtcac tcttcgccca gcttttcatt gagctggcgg
      541 aatggcatag cccactttat ctggtcatcg atgactatca tctgatcact aatccagtga
      601 tccacgagtc aatgcgcttc tttattcgcc atcaaccaga aaatctcacc ctggtggtgt
      661 tgtcacgcaa ccttccgcaa ctgggcattg ccaatctgcg tgttcgtgac caactgctgg
      721 aaattggcag tcagcaactg gcatttaccc atcaggaagc gaagcagttt tttgattgcc
      781 gtctgtcatc gccgattgaa gccgcagaaa gcagtcggat ttgcgatgac gtttccggtt
      841 gggcgacggc actatagcta atcgccctct ccgcccggca gaatacccac tcagcccata
      901 agtcggcacg ccgcctggcg ggaatcaatg ccagccatct ttcggattat ctggtcgatg
      961 aggttttgga taacgtcgat ctcgcaacgc gccattttct gttgaaaagc gccattttgc
     1021 gctcaatgaa cgatgccctc atcacccgtg tgaccggcga agaaaacggg caaatgcgtc
     1081 tcgaagagat tgagcgtcag gggctgtttt tacagcggat ggatgatacc ggcgagtggt
     1141 tctgctatca cccgctgttt ggcaacttcc tgcgccagcg ctgccagtgg gaactggcgg
     1201 cggagctgcc ggaaatccac cgtgccgccg cagaaagctg gatggcccag ggatttccca
     1261 gcgaagcgat tcatcatgcg ctggcggcag gcgatgcgct gatgctgcgc gatattctgc
     1321 ttaatcacgc ctggagtttg ttcaaccata gcgaactgtc gctgctggaa gagtcgctta
     1381 aggccctgcc gtgggacagt ttgctggaaa atccgcagtt agtgttattg caggcgtggc
     1441 taatgcaaag ccaacatcgc tatggcgaag ttaacaccct gctagcccgt gctgaacatg
     1501 aaatcaagga catcagagaa ggcaccatgc atgcagaatt taacgctctg cgcgcccagg
     1561 tggcgattaa cgatggtaat caggatgaag cggaacggct ggcaaaactg gcactggaag
     1621 agctgccgcc gggctggttc tatagccgca ttgtggcaac ctcggtgctg ggtgaagtgc
     1681 tgcactgcaa aggcgaattg acccgctcac tggcgctagt gcagcaaacc gaacagatgg
     1741 cacgccagca cgacgtctgg cactacgcct tatggagttt aattcagcaa agtgaaattc
     1801 tgtttgccca agggttcctg caaaccgcgt gggaaacgca ggaaaaagcg ttccagctga
     1861 tcaacgagca gcatctggaa cagctgccaa tgcatgagtt tctggtgcgc atccgagcac
     1921 aactgttatg ggcctgggca cggctggatg aagccgaagc gtcggcgcgt agcgggattg
     1981 aagtcttgtc gtcttatcag ccacagcaac agcttcagtg cctggcaatg ttgattcaat
     2041 gctcgctggc ccgtggtgat ttagataacg cccgtagcca gttgaaccgt ctggaaaacc
     2101 tgctggggaa tggcaaatat cacagcgact ggatctctaa cgccaacaaa gtccgggtga
     2161 tttactggca aatgaccggc gataaagccg ccgctgccaa ctggttgcgt catacggcta
     2221 aaccggagtt tgcgaacaac cacttcctgc aaggtcaatg gcgcaacatt gcccgcgcac
     2281 aaatcttgct gggcgagttt gaacctgcgg aaattgttct cgaagaactc aatgaaaatg
     2341 cccgcagtct gcggttgatg agcgatctca accgtaacct gttgctgctt aatcaactgt
     2401 actggcaggc cggacgtaaa agtgacgccc agcgcgtgtt gctggacgca ttaaaactgg
     2461 cgaatcgcac cggatttatc agtcattttg tcatcgaagg cgaagcgatg gcgcaacaac
     2521 tgcgccagct gattcaactt aatacgctgc cggaactgga acagcatcgc gcgcagcgta
     2581 ttctgcgaga aatcaatcaa catcatcggc ataaattcgc ccatttcgat gagaatttcg
     2641 ttgaacgtct gctaaatcat ccggaagtgc cagaactaat ccgcaccagc ccgttgaccc
     2701 aacgtgaatg gcaggtcctg gggctgatct actccggtta cagcaatgaa caaattgcgg
     2761 gcgaactgga agtggcggca accaccatca agacgcatat ccgcaatctg taccaaaaac
     2821 tcggcgtcgc ccaccgtcag gctgccgtgc agcacgcgca gaaactgctg aagatgatgg
     2881 gttatggggt gtgagtttag ccggataatg cattggatcc ggtttacatc tttacatcat
     2941 tcaatactca cccgcgttac gccatctgtt tctatcaaac caaaccgcac cggcaagaaa
     3001 cgctccacca ccgcgatatt ggtcagcaga tggcatgaag gatgggcgac cgtaaattct
     3061 cccgcgcccg ccagcgccat cggcagtacc agttggtcgg cgagatattc ccccaccgcc
     3121 gctgggcttg ccaggtagcg tttcacctct ttcactaact gcgcggctac cacctcggca
     3181 ctgacgcgct tttcaccgac gacgaaaaag cgttcagtga tattttcact ttcgacttcc
     3241 agcgagacgg tattacccgg cccctggtcg cgcggcaggt tatgaatatt ctgttcatgc
     3301 agggaaaaaa tccccgccag tgtagcgatt tcacgctcag caacatggcg cggcacacca
     3361 gctaatagaa cttctccacg catctgcaca atgttcccgc gctcgccaag ttgcaaggtg
     3421 ctaaacgatg ccacgggcga gacttccgtt gccaccacac cgcctccggc aggataaaaa
     3481 ccgtgacgta acagcgtggt ttgctgatga attcctattt tcgccagcag cggctccagc
     3541 acccggcgga taaaatccgc aggcggcgcc gacgggttat cggtgccgcc gctcacttca
     3601 acacgcgaag gtccatcggc aaaccacagc gcgggcagca ccgtttgcag caccagcgta
     3661 caacttccgg cgctaccgat agcaaagcgg taatcgccgc cgcgcacggt gccaggccgg
     3721 aagaccagac gctgcgatcc cagctccgcg ccttccaccg ttgccctaca aatttccgca
     3781 gccgctttta ccgcggtcag atgctggcgc agcagccccg gtttcgcccg tccggctcga
     3841 atgccagtga tggtaaatgg ctggccggtt atcatcgaca ggctcagcgc cgagcgcagg
     3901 atctgcccgc cgccttcgcc ctgtgcgcca tccagcgcaa tcatcctttt catcatttat
     3961 ccttttacgc acaccacctg acgcagggtg tagataactt ccaccagatc gctttgtgcc
     4021 gccatcaccg catcaatatc tttatacgcc atcgggattt cgtcgatcac ttcggcatct
     4081 ttacggcatt ccacatgcgc ggtggcacga atttggtctt ccacgctgaa cagttttttc
     4141 gctttagttc ggctcattac ccgcccggca ccgtggctgc acgaacagaa cgactcttca
     4201 tttcccagcc cacggacgat aaagcttttt gctcccatcg aaccgggaat aattccatat
     4261 tgaccagcac gcgcagacac cgcaccttta cgcgtcacgt agatctcttc accaaagagc
     4321 tgttcttttt gcacatagtt gtggtgacag ttgatctctt ccatcgccag cgtttgtggc
     4381 tgtctgaccg ttttctgcgt aatgctctgc aacgccgtta ccacattttc catcatcgca
     4441 tcgcggttaa ggctggcgaa aagctgcgcc caggccacgg ctcttaggta atcatcaaag
     4501 tattccgtac cttccataaa gtacgccaga tcacgcgacg gcaacgtctc aagttggtcc
     4561 tgcatctctt tttgcgccag atcgataaag taagtcccga tggcgttacc aattccgcgt
     4621 gaaccggagt gcagcataat ccacacctgc tccgactcat caaggcagat ttcaataaag
     4681 tggttacccg ttcccagcgt tcccaggtgt ttatagttgt tagtgttaag gaaacgcgga
     4741 tatttttgcg ttaaccactg ataaccggcc tccagctcag cccatttcgc atcgacgtta
     4801 aaaggcgggt tttcccatgc gcctttatca cgtttacaac gcccggtagt acgcccgtgt
     4861 ggtacggccg tttcaatcgc ttgacgcagc tccgccaggt tttcaggcag gtctgccgcc
     4921 gttaacgcgg tacgcagcgc gttcattcca cagccaatat ccacgcccac cgccgccgga
     4981 ataatcgccc ctttggtcgg gatcacgcta ccaatggtgg aacctttacc caggtgtaca
     5041 tcaggcatta ccgcaatatg tttgaaaata aacggcatct tcgccgtatt aataagttgc
     5101 tgacgcgcat cggcttctac cggcacgcct ttggtccaca tttttaccgg ggcattttca
     5161 gtggtcagta attcgtaatt cattttgttt ttctcttttc gttgtttgcg gtcctgataa
     5221 ttgcaaccgt cgtgccagaa aattaaataa gcagattaat ttattgtttc taaaaagatt
     5281 atatctttat atccgtaaac ggagatttcc cgcaaagccg atttaccgat aatgaaatat
     5341 cgccttttat aaggatatct aagatgcgta aaacagtggc ttttggcttt gtcggtaccg
     5401 tgctggatta tgccggacgc ggcagtcagc gctggtcaaa atggcgtccg tcactctgta
     5461 tataccagca agaatcgttg gtcatcgatc gactggaatt attgcacgat gcccgctcgc
     5521 gctcgctatt tgaaacgctt aaacgcgata tcgccagcgt ttcgccagaa acagaagtgg
     5581 tgggcgttga gattgaactg cataacccgt gggatttcga agaggtttac gcctgcctgc
     5641 atgatttcgc ccgtggttac gagtttcagc cagaaaaaga agactattta attcacatca
     5701 ccaccggcac ccacgtcgcg cagatttgct ggtttctgct ggcggaagcg cgttatctac
     5761 ccgcccggtt gatacaatct tcaactccgc gcaaaaaaga acagccgcgc ggcccaggtg
     5821 aagtgacgat tatcgatctc gatttaagcc gttataacgc catcgccagc cgctttgccg
     5881 aggaacggca gcaaacgctc gatttcctca aatccggtat tgccacgcgt aacccccact
     5941 tcaaccgcat gattgagcag atcgaaaaag tggcgattaa atcccgcgcg ccgattctgc
     6001 ttaacggccc gaccggcgca ggtaagtcat ttctggcgcg acgcatcttc gagttaaaac
     6061 aggcacggca tcagtttagc ggcgcgtttg tggaagtgaa ctgcgccacc ctgcgcggcg
     6121 ataccgcgat gtcgacgctg tttggtcatg tgaaaggcgc gtttaccggg gcgcgggaat
     6181 ctcgtgaagg attattacgc agcgccaacg gcggaatgtt gtttcttgat gagattggcg
     6241 aactgggcgc agacgaacag gcaatgctgc tgaaagctat tgaagagaaa accttttacc
     6301 cgtttggcag cgatcgccag gtgagcagtg attttcagct tatcgccgga acggtgcgcg
     6361 atttgcgcca gctggttgcc gaaggcaaat tccgcgaaga tctgtacgcg cggatcaatc
     6421 tctggacctt caccctgccg ggtctacgcc agcgccagga agatattgaa ccgaacctgg
     6481 attatgaagt ggagcgccac gccacactca ctggtgacag cgtgcgtttt aacaccgaag
     6541 cgcggcgtgc ctggctggcc tttgcaacat ctccccaggc aacatggcgc ggtaactttc
     6601 gcgaactttc tgccagcgtc acgcggatgg ccacctttgc cactagcgga cgcatcactc
     6661 tggacgtggt ggaagatgag ataaaccgtc tgcgctataa ctggcaggag agccgcccct
     6721 ccgcgcttac ggcgttgctg ggcgcagagg cagaaaacat cgatctcttc gaccgtatgc
     6781 aactggaaca cgttatcgct atctgccgcc aggcaaagtc gctttctgct gccggacgac
     6841 agctttttga tgtttcgcgc cagggcaaag ccagcgtcaa tgacgcggat cggctacgca
     6901 aatacctggc gcgttttggt ctgacgtggg aagccgtgca ggatcagcac agctccagtt
     6961 gaatatggtg gtccgtcagc acctgcatca cgcttactgg cggcggggcg tcggtgtaga
     7021 cggcatcaac catgctgatg ctccccatat tgaccatcgc attacggcca aattttgaat
     7081 ggtcaaccac cagcataacg tggcgcgagt tctcaataat ggcgcgcttg gtacgaactt
     7141 cgtggtaatc gaactccagc agcgagccgt cgctgtcgat gccgctgatc cccagaatgc
     7201 cgaaatcaag gcggaactgg gagataaaat cgagcgtcgc ttcgccaatg atcccgccat
     7261 cgcggctacg taattcgcca ccggcgagaa taatgcggaa atcttctttt accatcaacg
     7321 tgttagcaac gttgagattg ttggtgacaa tgcgcaaatt gctgtgattg agcagcgcgt
     7381 gcgccaccgc ttccggcgtg gtgccgatat cgataaacag cgtcgagcca ttggggattt
     7441 gctccgccac tttgcgggcg atgcgctctt tttcttcggt ctgggtggct ttacgatcgt
     7501 gccacggcgt gttaaccgaa ctggaaggca gcgccgcgcc gccatgatgg cgcaggatca
     7561 ggttttgctc tgccagctcg ttgaggtcgc ggcgaatagt ctgcgggctg acggagaagt
     7621 gctccaccag ttcctcggta ctgacataac cctgctgttt aaccagttcg ataataccgt
     7681 tgtgacgttg tgtttgtttc atttataaat ccctggaatt attttcgttt tcgcgcattg
     7741 agcgaatcaa caaaagccat cgctaaaccc acggctaatc cggcgatgtg tgctccgttc
     7801 gccatcgaca tcccaaacaa atcaaaccat ccggcgacaa tccagatcag cgcaaagata
     7861 attaacccac gttgcaggta aatgccactt tgcggatcgc gttcgccacg tagccagacg
     7921 tagcccatca gcgcatacac cacgccagaa agcccgccaa accacggccc gctgaatttt
     7981 tgctgcatat agccgcttaa cagggcgctg ataagcgtaa tgacaattag cttaccgcta
     8041 ccgaggcgtt tttccaccgc gccgccgaga taccaccacc aaagcaggtt aaagaggata
     8101 tgcatcagcg agaagtgcat taacgcgtgg gtgaagtaac gccagaactc aaatttcagt
     8161 gttggatcga atggccaggc cagccataac atcacttcct gatcgccgag aatttgcatg
     8221 gcaataaaca ccaccacgca ggcgatcatc atcacccagg tcaccggacc tgcgcgttca
     8281 cgcaaggcag caaagaaagg ataacggcga taatgcaggc cactgccggt atggcctgcc
     8341 agccagctgg ccgccagata acgcggatct gccgggtttt cgagaaaacg cgccagctcc
     8401 gcccgcacgc gctcggcctg ggactcatcc gccagccaga catcgctttg gttatgttgt
     8461 tgaatcgtga ggataacacc ctgcgtcgcc atgtaatcaa caaacgcctg cgccacgcgg
     8521 gggttagcaa aagaggtaat catcaacatc gttgctgtcg cttattccac acaaaagggg
     8581 acagtataaa gcgttacgcg ccgtacgcca cctctgcggg aaactgtcgc tgccaggctt
     8641 caaagccgcc gtcaatgcta tagaccacat cgtagccctg ttgcagcaga tactgcgccg
     8701 cccctttgct gctattgccg tgataacaca tcaccatcac cggagtgtca aagtcgttat
     8761 cacgcataaa cgcgcccagc gtgtcgttgg ttaaatggaa agcctgcacc gcatgcccca
     8821 tagcgaaact ctgtggatcg cgaatatcga ccagcaccgc ctctttttcc tgcaacttct
     8881 ggtgcgcgtc ggcaacgtta atacattcga actgatccat gcgtctctct ttctttacaa
     8941 acaagtgggc aaatttaccg cacagtttac gtcgaagcgg cagataaacg ccataatgtt
     9001 atacatatca ctctaaaatg ttttttcaat gttacccaaa gcgcgattct ttgctaatat
     9061 gttcgataac gaacatttat gagctttaac gaaagtgaat gagggcagca tggaaaccaa
     9121 agatctgatt gtgataggtg gcggcatcaa tggtgctggt atcgcggcag acgccgctgg
     9181 acgcggttta tccgtgctga tgctggaggc gcaggatctc gcttgcgcga cctcttccgc
     9241 cagttcaaaa ctcattcacg gtggcctgcg ctaccttgag cactatgaat tccgcctggt
     9301 cagcgaggcg ctggctgaac gtgaagtgct gctgaaaatg gctccgcata tcgccttccc
     9361 gatgcgtttt cgcctgccac atcgtccgca tctgcgcccg gcgtggatga ttcgcattgg
     9421 tctgtttatg tacgatcatc tgggtaaacg caccagcctg ccgggatcaa ctggtttgcg
     9481 ttttggcgca aattcagtgt taaaaccgga aattaagcgc ggattcgaat attctgactg
     9541 ttgggtagac gacgcccgtc tggtactcgc caacgccctg atggtggtgc gtaaaggcgg
     9601 cgaagtgctt actcggactc gcgccacctc tgctcgccgc gaaaacggcc tgtggattgt
     9661 ggaagcggaa gatatcgata ccggcaaaaa atatagctgg caagcgcgcg gcctggttaa
     9721 cgccaccggc ccgtgggtga aacagttctt cgacgagggg atgcatctgc cttcgcctta
     9781 tggcattcgc ctgatcaaag gcagccatat tgtggtgccg cgcgtgcata cccaaaagca
     9841 ggcctacatt ctgcaaaacg aagataaacg cattgtgttc gtgatcccgt ggatggacga
     9901 gttttccatc atcggcacca ccgatgtgga atacaaaggt gatccgaaag cagtgaagat
     9961 tgaagagagt gaaatcaatt acctgctgaa agtgtataac gcgcacttta aaaagcagct
    10021 aagccgtgac gatatcgtct ggacctactc cggtgtgcgt ccgctgtgtg atgatgagtc
    10081 cgactcgccg caagccatta cccgtgatta cacccttgat atccatgatg aaaacggcaa
    10141 agcaccgctg ctgtcggtat tcggcggtaa gctgaccacc taccgaaaac tggcagaaca
    10201 tgcgctggaa aaactgacgc cgtattatca gggtattggc ccggcgtgga cgaaagagag
    10261 cgtgctgccg ggtggtgcca ttgaaggcga ccgcgacgat tacgccgctc gcctgcgccg
    10321 ccgttacccg ttcctgactg aatcgctggc acgtcactac acgcgcactt acggcagcaa
    10381 cagcgagctg ctgctcggca atgcgggaac ggtaagcgat ctcggggaag atttcggtca
    10441 tgagttctac gaagcggaac tgaaatacct ggtggatcac gaatgggtcc gccgcaccga
    10501 cgatgccctg tggcgtcgca caaaacaagg catgtggctg aatgcggatc aacaatctcg
    10561 tgtgagtcag tggctggtgg aatatacgca gcagaagttg tcgctggcgt cgtaaattaa
    10621 cgtaaggtga tcaggtcaga tttcaatctg gcctgagact gatgacaaac acaaaactgc
    10681 ctgatgcgct acgcttatca ggcctacgtg gtttatgcaa tatattgaat ttgcatggtc
    10741 ttgtaggcca gataagacgt tcacgtcgca tccggcatga acaaagcgca ctttgtcaaa
    10801 aatctaacct actttttaat tcaggaaatt accgcaaagc ccacgccatc atacccaacg
    10861 taacaaaact caggcacgtt ccccactcgc cccgagaaaa atagcattaa tgcgcccagc
    10921 gtcagcataa aaatattgag cagaaggtaa attgttggcg taaagagtaa attgcaaacc
    10981 tcaggaatag cggcaagccc agcccccagc atggcaataa ccgctgcaaa aaattgcgtc
    11041 agtattggga tgccgaaaaa agtcattacc agcgaggtca aaatccattt tctattttgc
    11101 attattcttt ccattctttc tgaatggcga aattatactc cccgagtccc cttgcccctt
    11161 ctggacactt ttccgaaatg atggcgaaaa aaacgggacc ctttgg
//
LOCUS       DAZPEU010000125        11143 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPEU010000125 DAZPEU010000000
VERSION     DAZPEU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.126"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            42..146
                     /gene="speFL"
                     /locus_tag="ACHU2L_002880"
     CDS             42..146
                     /gene="speFL"
                     /locus_tag="ACHU2L_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR1357652.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            457..2654
                     /gene="speF"
                     /locus_tag="ACHU2L_002881"
                     /pseudo
     CDS             457..2654
                     /gene="speF"
                     /locus_tag="ACHU2L_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2651..3970
                     /gene="potE"
                     /locus_tag="ACHU2L_002882"
     CDS             2651..3970
                     /gene="potE"
                     /locus_tag="ACHU2L_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR1357653.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4026..5666)
                     /gene="pgm"
                     /locus_tag="ACHU2L_002883"
     CDS             complement(4026..5666)
                     /gene="pgm"
                     /locus_tag="ACHU2L_002883"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR1357654.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5692..6237)
                     /gene="seqA"
                     /locus_tag="ACHU2L_002884"
     CDS             complement(5692..6237)
                     /gene="seqA"
                     /locus_tag="ACHU2L_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR1357655.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6422..7186
                     /gene="ybfF"
                     /locus_tag="ACHU2L_002885"
     CDS             6422..7186
                     /gene="ybfF"
                     /locus_tag="ACHU2L_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1357656.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7326..7619
                     /gene="ybfE"
                     /locus_tag="ACHU2L_002886"
     CDS             7326..7619
                     /gene="ybfE"
                     /locus_tag="ACHU2L_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR1357657.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7759..8289
                     /gene="fldA"
                     /locus_tag="ACHU2L_002887"
     CDS             7759..8289
                     /gene="fldA"
                     /locus_tag="ACHU2L_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR1357658.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8499..8585
                     /gene="uof"
                     /locus_tag="ACHU2L_002888"
     CDS             8499..8585
                     /gene="uof"
                     /locus_tag="ACHU2L_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR1357659.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8578..9024
                     /gene="fur"
                     /locus_tag="ACHU2L_002889"
     CDS             8578..9024
                     /gene="fur"
                     /locus_tag="ACHU2L_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR1357660.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9108..9434)
                     /gene="chiQ"
                     /locus_tag="ACHU2L_002890"
     CDS             complement(9108..9434)
                     /gene="chiQ"
                     /locus_tag="ACHU2L_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR1357661.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9471..10877)
                     /gene="chiP"
                     /locus_tag="ACHU2L_002891"
     CDS             complement(9471..10877)
                     /gene="chiP"
                     /locus_tag="ACHU2L_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR1357662.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2714 a         2756 c         2784 g         2889 t
ORIGIN      
        1 ataactctac cttcaactga cacagcaagc ggtaaaggta aatggaaaat aataaccgct
       61 taatgcctca tataaggcgg acaacccata tcatgaagtt tacccatcgt aatagttttg
      121 actttcattt ctttaatgcc cgctagtctt ctgactaaag ggcaccccaa cgtacaggtc
      181 tccctgactt taagcattac aggttaatac ctgtattcct cggtgctcat atactgctaa
      241 cccttttaaa ctctaaataa ttcgagtcgc agcacttgca acttgaggta tgacgagtat
      301 agccagttac cgggctggtc tgggttattg catctgcaaa aagcaaacta ctgatttatt
      361 tatcagcggt ggagctttgc tttttttccg acgtgatcga tttctccttt gagaaattga
      421 ggacctgcta ttacctgaaa taaagagatg aataaaatgt cagaattaaa aattgccgtt
      481 agccgttctt gcccggattg tttttccact catcgtgcat gcgtgaatat tgacgaaagt
      541 aattatattg acgttgccgc cattatttta tcagtcaatg atgttgaacg tggaaaactc
      601 gataaaatag acgctactgg ctatggcatt cctgttttta ttgcaacgga aaatgaagaa
      661 cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg cgaatcacgg
      721 aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac gcaactgcgc
      781 ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc ttttgactgc
      841 ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca gttcgtggag
      901 tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc aatgggcgat
      961 ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa agtgtttaat
     1021 gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt ggttttaaac
     1081 gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa atctaaccac
     1141 cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc acgtaacccg
     1201 tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct gcgtgagctg
     1261 atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct cgctgtgatt
     1321 cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga taaaattggt
     1381 catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca atttatcccg
     1441 atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc gggtattcta
     1501 gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca aatccataaa
     1561 aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat gaacaacgcc
     1621 tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact ggatatcaac
     1681 gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt ggtaaatggc
     1741 attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt cgtacctgaa
     1801 ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt tgatctgcgc
     1861 ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc agagaatcaa
     1921 tactttgtcg atccttgcaa actgttgctg acaaccccag gtattgatgc acgtaacggc
     1981 gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg cgaaaatggc
     2041 gtagtgccgg aaaaatgcga tcttaacgcc atcctcttcc tgctgactcc ggcagaagat
     2101 atggccaaac ttcagcaact tgtgccctgc tggtacgctt cgaaaaactg cttgagtccg
     2161 acgcgccatt agcagaagtg ctgccttcca tctacaaaca gcatgaagag cgctacgccg
     2221 gttataccct gcgtcagttg tgccaggaaa tgcatgattt gtatgcccgc cacaacgtga
     2281 aacaactgca aaaagagatg ttccgcaaag cgcacttccc acacgtcagc atgaatccgc
     2341 aagaagccaa ctacgcctat ttacgcggtg aagtggagct ggttcgtcta ccggatgccg
     2401 aaggtcgtat cgctgccgaa ggggcgcttc cttatcctcc gggtgtgctg tgtgttgttc
     2461 cgggtgaaat ctggggtggt gctgttttgc gttacttcag cgctctggaa gaagggatca
     2521 acctgctgcc aggttttgca ccggagctgc agggtgtcta tatcgaagaa catgatggtc
     2581 gtaagcaagt ttggtgctat gtcatcaagc ctcgtgatgc gcaaagcacc ctgttgaaag
     2641 gggaaaaatt atgagtcagg ctaaatcgaa caaaatgggc gtcgttcagt taaccatact
     2701 gacgatggtc aacatgatgg gctccggtat catcatgctg ccgacaaagc ttgccgaagt
     2761 tgggacaatt tcaattatct cctggctggt gacagccgta ggctcaatgg cactggcatg
     2821 ggcattcgca aaatgcggta tgttcagccg taaatcaggt ggtatgggcg gttatgccga
     2881 gtatgctttc ggtaaatccg gtaactttat tgcgaactat acctacggcg tctcactgct
     2941 gattgctaac gtcgcgattg ctatttcggc ggttggttac ggcaccgaac tgctcggcgc
     3001 gagtttgtcg ccagtgcaga ttggtcttgc gaccatcggg gtgctgtgga tttgtaccgt
     3061 ggctaacttt ggtggtgcgc gcattaccgg gcaaatcagt agcattaccg tgtggggggg
     3121 cattattccg gtggtaggcc tgtgcattat cggctggttc tggtttagcc cgacgcttta
     3181 cgttgattcc tggaatccgc atcatgcacc gttcttcagt gcagtaggtt cttccatcgc
     3241 tatgacgctg tgggcttttc ttggtctgga gtctgcgtgt gcgaatactg atgtagtgga
     3301 aaacccggaa cgtaatgtgc caatcgcggt actcggcggt acgttaggtg cggcggtgat
     3361 ttatatcgtc tccaccaacg tgattgcagg gattgtgcct aatatggagc tggcaaattc
     3421 aacggcacca tttggcctgg ccttcgcaca gatgtttaca ccagaagtgg gtaaagtcat
     3481 tatggcgctg atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt tcaccattgc
     3541 ccaggtgttt aaatcttcat ctgatgaagg ctacttccct aaaattttct cccgtgtaac
     3601 caaagtggat gcaccggtgc agggaatgtt gaccattgtg attatccaga gcgggttggc
     3661 gctgatgacg attagcccgt cgctaaacag tcagttcaac gtgctggtta acctggccgt
     3721 ggtgaccaat atcattccgt atattctgtc aatggcggca ttagtcatta ttcagaaggt
     3781 cgctaatgtg cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat
     3841 gtatagcttt tatgcgctgt actcatccgg ggaagaagcc atgctgtacg gttccatcgt
     3901 gaccttcctt ggttggacac tgtatggtct ggtatcacca cgctttgaac tgaaaaataa
     3961 acacggttaa aaaagcaaag taaaaaaggg cgatcttgcg accgctcttt ttttattaaa
     4021 tgtgtttacg cgtttttcag aacttcgcta acaatctcaa ccgcttcttt ctcaatctgc
     4081 ttgcgatgtt cttcaccgag gaagctttcg cagtagatct tatatgcgtc ttccgtgcct
     4141 gacggacgcg cggcgaacca gccgttgtca gtcatcactt tcagaccgcc aatagaagca
     4201 ccgttgcccg gagcagcagt caggcgcgcg gtgatcgggt cacctgccag ggtgctggcg
     4261 ctcaccattt ccggagacag cttagacagc gccgcttttt gtgcggaagt cgcagctgcc
     4321 tgcaaacggt tgtagctcgg cgcaccaaag cgttttgcca gttcgttgta gtgttcctgc
     4381 gggttcttac cggtgacagc ggtgatttcc gccgccagca gacacataat gatgccgtct
     4441 ttgtcggtgg accacggcgt gccgtcgaaa cgcaggaagg aagcccctgc gctctcttcg
     4501 ccgccaaagc cgaagctgcc gtcgaacaga ccatcaacaa accatttgaa acctaccggt
     4561 acttctacca gcttacggcc caagtcattg accacacggt cgatcatcgc agaagaaacc
     4621 agcgttttac cgacagcaac atctttgccc cactgcggac gatgctggaa caggtagttg
     4681 atcgccaccg ccaggtagtg gttcggattc atcaaacctg ccggagtgac gataccgtga
     4741 cggtcataat ccgggtcgtt agcaaacgcc agatcgaact tatcacgcag tgccagcaga
     4801 cccgccatcg cacactcgga ggagcagtcc atacggatcg caccgtcttt atcgaggtgc
     4861 ataaagcgga aggtttgatc gacctgatcg ttaacgatgg tcaggttgag gttgtaatac
     4921 tcgccaatgc gcttccagta ttcgataccg gaaccgccca acggatcaac gcccagcgtc
     4981 aggcccgctt tctgaatcgc cgccatatca acgatatcgg ccagcccttc cacgaacggc
     5041 tgcaccagat cctgctcttt cacatagccg gatgccatcg cttcgtcgag ggagatacgc
     5101 ttcacgcctt tcaggccatc ggccatcagt gcgttggcct tgtcttccac cactttggtg
     5161 acgttagtat cagccgggcc accatttggc ggattgtact tgataccacc atcttccggc
     5221 gggttatggg acggtgtaat cacgataccg tctgccagcg ggccaccttt tttattgtga
     5281 accaggatgg cattggaaac ggcaggcgtc ggagtgaagc cattgttttc ctgcacaatg
     5341 acatcaacgc cgttcgctgc cagcacttcc agtacggaaa tgaacgcagg ctcggacagg
     5401 gcgtgagtat ctttacccac atagcaaggg ccagtgatgc cgtttttcgc acgttcttca
     5461 gcaattgcct gagcgatcgc cagaatgtgc ggctcgttaa agctgtggcg cgctgcactg
     5521 ccacggtgac cggaagtacc gaatttcacc gcgtgctccg cattccctgc ttctggtttc
     5581 agtacataat attgcgccgt cagttgggcg acgttaatca aatcactctg ttgtgcaggt
     5641 tggcctgcac gattgtggat tgccattgct ttgtcctttg tctgcaacgt tttagatagt
     5701 tccgcaaacc ttctcaatca attccgccgg gaattgcatc gactgcatga tgtgttcgat
     5761 catgctgcat ttacggccgg tgttggtgtt ggtgatcacc caatacggcg tgcctggcac
     5821 atgtttcggc ttggtctgat taccattttt cagcagcgtt tgttcatctg ccgcaaagta
     5881 cacgcgtgta cgaccgtgca acgattccgt tgcttcggca aacgcctggg cgtcaagaga
     5941 atatagtgta gacaacagca gcataaagcg attgaccgct cgcttttgct ctgcgtattc
     6001 gtccgaaagc agaagttcac gcattgcgcg aaccttgtct ttaatcgttt tgaccggctt
     6061 cgcttcgacg atagcaggtg acgcaacgcg aacctctttc gtcaccggag cagcaggctg
     6121 tgatgcggcg gaaaatttca acatacgccg taaaatgtcg gatgcgctct cgccgatatg
     6181 cttagtgtgg ctggcaatat agctgtagag ttcatcatca acttcaatcg ttttcatctt
     6241 aatccagtgc agtgtcttag ctgaatacaa gtcgccaaga gtatatgaat aaatcccggc
     6301 agcggatagc gtcaagcctg gcttgcagaa aaactcggaa taaaccttat ttgttgcagc
     6361 cctgtggcac aatggtcaac ataataccct aacccgacaa cgcgaaaaaa agaactttgc
     6421 catgaaatta aatatccgcg cgcaaactgc acaaaaccag cacaataatt ctcccatcgt
     6481 tcttgtccat ggtctgtttg gtagcctcga caaccttggc gtactggctc gcgatctggt
     6541 aaacgatcac aatatcatcc aggttgatat gcgtaaccac ggtctttcac cgagagagcc
     6601 ggtaatgaat tacccggcga tggcgcagga tcttgttgat accctggatg cactgcagat
     6661 cgacaaagca acatttatcg gtcactccat gggcggtaaa gcggtaatgg cacttactgc
     6721 actggccccc gatcgcatcg ataaactggt ggcgatcgat atcgcaccgg tcgactatca
     6781 cgtacgccgt catgatgaga ttttcgcagc tatcaacgcg gtcagtgaat cggacgcaca
     6841 aactcgccag caagcagcag caataatgcg ccagcatctt aatgaagaag gggtgattca
     6901 gtttctgttg aaatcttttg ttgacgggga gtggcgcttt aacgtgccgg tattgtggga
     6961 tcagtatccg catattgtgg gttgggagaa aatcccggca tgggatcacc ctgccctgtt
     7021 tatccctggc ggcaattctc cgtatgttag cgagcagtac cgtgatgatt tactggcaca
     7081 atttccacag gcacgggcgc atgtgattgc aggcgcgggt cactgggtcc atgctgaaaa
     7141 accggatgcg gtattacgcg ctatccgtcg ctatctcaat gattaacgca actgattaaa
     7201 aacccagcgt ccgcgtcgct ttccgcgcgt gggcgttggc gtgactcatc gcgctgatgt
     7261 attatggcgc gctatccatc cgggcagagg cctggctgat tgtttccccc gaagtcacca
     7321 agatcatggc caaagaacaa acggaccgta cgacattaga tctgttcgcg cacgagcgtc
     7381 gaccgggacg accgaaaact aatccgcttt cgcgcgatga acagctgcgt attaataaac
     7441 gcaaccagct aaaacgcgac aaagtacgtg gccttaagcg tgtcgaactg aagctgaacg
     7501 cggaagctgt cgaggcgctg aacgagctgg cggaatcgcg taatatgagc cgtagcgaac
     7561 tgatcgaaga gatgttaatg cagcaactgg cggcgctgcg tagtcagggc atcgtttaaa
     7621 tttccacttt catgtagcac agtgtgcagt cctgctcgtt tgctgattcc gcaagactgc
     7681 ctgttctgct atgattgcct ttatccgtgg gcaattttcc acccccattt caataagttt
     7741 caagaggtta tttcactcat ggctatcact ggcatctttt tcggcagcga caccggtaat
     7801 accgaaaata tcgcaaaaat gattcaaaaa cagcttggta aagacgttgc cgatgtccat
     7861 gacattgcaa aaagcagcaa agaagatctg gaagcttacg acattctgct gctgggcatc
     7921 ccaacctggt attacggcga agcgcagtgt gactgggatg acttcttccc gactctcgaa
     7981 gagattgatt tcaatggtaa actggttgcg ctgtttggtt gtggtgacca ggaagattac
     8041 gccgaatatt tctgcgacgc attgggcacc atccgcgaca tcattgaacc gcgcggtgca
     8101 accatcgttg gtcactggcc aactgcgggc tatcatttcg aagcatcaaa aggtctggca
     8161 gatgacgacc actttgtcgg tctggctatc gacgaagacc gtcagccgga actgaccgct
     8221 gaacgtgtag aaaaatgggt taaacagatt tctgaagagt tgcatctcga cgaaattctt
     8281 aatgcctgat gtgatgcggc gtagactcat gtctacgccg tattaataga taatgccaat
     8341 caaaataatt gctacaaatt tgtaactttt gctgttgtac ctatacaatg tcccagtgtt
     8401 caagtggcct tgccgttgta aatgtaagct gtgccacgtt tttattaaca atatttgcca
     8461 gggacttgtg gttttcattt aggcgtggca attctataat gatacgcatt atctcaagag
     8521 caaattctgt cacttcttct aatgaagtga accgcttagt aacaggacag attccgcatg
     8581 actgataaca ataccgccct aaagaaagct ggcctgaaag taacgcttcc tcgtttaaaa
     8641 atcctggaag ttcttcagga gccggacaac catcacgtca gtgcggaaga tttatacaaa
     8701 cgtctgatcg atatgggtga agaaattggt ctggctacgg tatatcgcgt actgaaccag
     8761 tttgacgacg ctggtatcgt cacccgccac aattttgaag gcggtaaatc cgtatttgaa
     8821 ctgacacagc aacatcacca cgatcacctg atttgcctcg actgcggcaa ggttatcgaa
     8881 tttagtgatg attccatcga agcgcgtcag cgtgaaattg ccgcaaaaca tggcattcgc
     8941 ctgactaacc acagtctcta tctttacggt cactgtgccg aaggcgattg ccgcgaagat
     9001 gagcacgcgc acgaaggcaa ataagccagc ctgaacgaga aaagccaacc tgcgggttgg
     9061 cttttttata caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta
     9121 ctgcgtattt cctgccagac cttatcacaa tcggctttca ccgcctgatc atttccggtt
     9181 tgcgttgcac gcagacactg ctgataatcc agtacgcgta cactttcctg gtcggcaaac
     9241 tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt
     9301 tctggcgaac cttgcgcggt gttgatacag gcgctgtatg cctctttcag acggctgtct
     9361 tcctgtggtg cggttgattg cgcacaagct accagccccg atgccatcat ggcgatgaga
     9421 atcagttttt tcattgaatg cggcaggcac aaaacctgcc gcgtcgggca tcagaagatg
     9481 gtgaatggtg cgattaccat aaatttcacg tcacgctcat cctggaagat gttaccgtaa
     9541 ccgccgcccc agcttgggat gtcggagtgg ttgtcgtatt cggtgaagtg cagtttgaac
     9601 atcgtgcctt tggcgcgacc gtcctgaatg gtgtagaccg catccaggct gtaggcagac
     9661 tcttcaatag tccggttttt gtcgtagtac gcatccggat tgctctgcca ggtcgcaggt
     9721 ttagcatccc atgcgtaaac gtaggaagcg ccgatggcga agcctggaag attccagttt
     9781 ttcaggtcat acatcgcact gaagaagacc gctttttcgc cgttagcgtt gaagtcggaa
     9841 cggttatccc accagatatc caggcggccg ttggaggaag cgtaggttgg ggtcatacgt
     9901 tgcaggaagt atccctgctg actgtcagcc ttaatccagg tgccttcgag gcgcaaatcc
     9961 actacgtcag ccgcccggta accaaaggtc aacgcttgca gccaggcggt gccgtcataa
    10021 agatcgttga cgctgcgatc gtcaacttta tcgcgggtac catagaactg gtagctggtg
    10081 gttaacgggc taccggcgat atcaaatttg tagctggctt tggcaaaata ttgatcgata
    10141 tacccttccg cctgaccaaa tgccgcttcc agtacgaagt tatttttgaa gtcgtatttc
    10201 gcaccgatgg agtgcagata atcaactttg gtggttttat cgttctgata aaactcatcc
    10261 atttccagat gccacggcgc tttgtattcg ttggtccaca tgtaggagaa actcaaggca
    10321 ccagcatcgc cgtaatcaaa attcgccccg gcttccgccc cctgataagt acccggcata
    10381 aagctccagt gcggcgctaa cagcgtttga ccggttggct gaatgtaacc tgccctcgcc
    10441 caaaccggac cgtatttaaa tttggccgca gctttataca ggcttatacc gcttttgtcg
    10501 ccggaccagt cttcgtcata ggctttatta ctttttgaaa acgcgatttc gttcgggtgg
    10561 gagctgtcgc cgttttccgc catttcaatc gccgtaaacg cagcaatatc aaggccgaac
    10621 atatcagcag cataaccgga ctgaaaatcg aggttggcat tccaggtgga gtgagaaagg
    10681 ttggttttgt atttgtcgcc gtcggtaaca tctttacggt cgcgttcacg ctgccagtaa
    10741 tagataccgc cggttaaggt tgaatcgtcg atgaatcctt cggcgcgagc ctccggcatt
    10801 gccataaagc ccgacattgc tgtaacaccg gcaatagcca gcgccagcgt actacgtttg
    10861 ccactaaacg tacgcatggg ttaatcctct ttgacgtata aattgctgca ccaaaggtga
    10921 acagcgaaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc tgactacagg
    10981 aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga atgtaagaac
    11041 atgagcaacc gcacatattt tgatgatttt tgttacaagg cttaagtttg cggttgaaag
    11101 gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aag
//
LOCUS       DAZPEU010000126        11047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPEU010000126 DAZPEU010000000
VERSION     DAZPEU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.127"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            110..1009
                     /gene="lysO"
                     /locus_tag="ACHU2L_002892"
     CDS             110..1009
                     /gene="lysO"
                     /locus_tag="ACHU2L_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR1357663.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1153..2805
                     /gene="hcp"
                     /locus_tag="ACHU2L_002893"
                     /gene_synonym="priS"
     CDS             1153..2805
                     /gene="hcp"
                     /locus_tag="ACHU2L_002893"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR1357664.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2817..3785
                     /gene="hcr"
                     /locus_tag="ACHU2L_002894"
     CDS             2817..3785
                     /gene="hcr"
                     /locus_tag="ACHU2L_002894"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR1357665.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3918..5636
                     /gene="poxB"
                     /locus_tag="ACHU2L_002895"
     CDS             3918..5636
                     /gene="poxB"
                     /locus_tag="ACHU2L_002895"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR1357666.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5673..6674
                     /gene="ltaE"
                     /locus_tag="ACHU2L_002896"
     CDS             5673..6674
                     /gene="ltaE"
                     /locus_tag="ACHU2L_002896"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR1357667.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6685..8115
                     /locus_tag="ACHU2L_002897"
     CDS             6685..8115
                     /locus_tag="ACHU2L_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR1357668.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8214..9227
                     /locus_tag="ACHU2L_002898"
     CDS             8214..9227
                     /locus_tag="ACHU2L_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1357669.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9224..10054)
                     /gene="amiD"
                     /locus_tag="ACHU2L_002899"
     CDS             complement(9224..10054)
                     /gene="amiD"
                     /locus_tag="ACHU2L_002899"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR1357670.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10051..10374)
                     /locus_tag="ACHU2L_002900"
     CDS             complement(10051..10374)
                     /locus_tag="ACHU2L_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR1357671.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10585..>11047)
                     /locus_tag="ACHU2L_002901"
     CDS             complement(10585..>11047)
                     /locus_tag="ACHU2L_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1357672.1"
                     /translation="VDMIRVNLSKCLFHECSMHGVKIKPWLPVKWTKELISDYLYGCL
                     LSLYSICARDIYNMNAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAFIYEFARMI
                     FAYINDKSIEILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         2539 a         2921 c         2896 g         2691 t
ORIGIN      
        1 cccctctgat gcataacatt tccttttaat tgcaacttta ctcgcccttc cgctttcagt
       61 agtgtgtcat cgcgcatttc gacttcttct ttgcaaggac tggattttca tgttttctgg
      121 gctgttaatc attctggttc ccctgattgt gggttacctc attccgcttc gccaacaagc
      181 tgcgttaaaa gctattaatc agctattaag ctggatggtt taccttattc tcttttttat
      241 gggtatcagt ctggcgtttc tcgataacct cgccagtaac ctgttggcga ttctgcatta
      301 ttctgccgtc agtattaccg ttattttact gtgtaatatt gccgccctga tgtggctgga
      361 gcgaggcctg ccgtggcgca accaccatca gcaagaaaaa ctcccgtcgc gtattgcgat
      421 ggcgctggag tcgctaaaac tgtgcggcgt agtagtgatt ggttttgcca ttggtctgag
      481 tggactggct ttcttacaac acgcgaccga agccagtgaa tacacgttaa ttttgctact
      541 tttcctcgtt ggtattcagt tgcgcaataa tggcatgacc ttaaagcaga ttgtccttaa
      601 tcgccgggga atgattgtcg ccgtggtggt ggttgtcagt tcattaattg gtggtttaat
      661 taacgccttt attcttgatc tccccatcaa taccgcgctg gcaatggcct ccggtttcgg
      721 ctggtattct ctttccggta ttttattgac cgaatctttt ggtccggtaa tcgggagcgc
      781 ggcgtttttt aatgatctgg cccgtgaact gattgctatt atgttgatcc ctgggctgat
      841 tcgccgcagc cgctctactg cactgggctt atgcggtgcc acatcaatgg atttcaccct
      901 gcccgttctt caacgtactg gcgggctgga tatggtcccg gcggcaattg ttcacggttt
      961 tattcttagc ctgttagtgc cgatcctcat cgcctttttc tctgcgtaat acctctctgg
     1021 cggtagttcc ctgccgccaa aattgcgcta aatcaatctc ccttaaagtt gcatgaaaaa
     1081 tcccttttat ccccgcgtta agcgtcttac ccttaaacat gtatattaaa tataacttta
     1141 aaaggtgtga tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc ggcagaaaac
     1201 ggctgctcat acgcgcaggg gatgtgtggt aaaacggcgg aaacttctga ccttcaggat
     1261 ttactcatcg cggcgctgca agggctttct gcctgggcgg taaaagcgcg tgaatacggc
     1321 atcatcaacc acgatgttga cagcttcgcg ccacgtgcct ttttctcaac cctgaccaac
     1381 gttaacttcg attctccgcg tattgtcggc tacgctcgtg aagcgattgc cttgcgcgag
     1441 gcgctgaaag cacaatgcct ggctgtagat gccaacgcgc gcgtcgataa cccgatggct
     1501 gacctgcaac tggtgagcga tgatctcggc gagctgcaac gtcaggcagc agaatttacc
     1561 cctaacaaag ataaagcggc gattggcgaa aacattctcg gcctgcgtct gctgtgcctg
     1621 tatggcctga aaggtgcggc ggcctatatg gaacacgcgc atgttctcgg tcaatacgac
     1681 aacgatattt atgcccagta ccataaaatc atggcgtggc tggggacctg gcctgccgat
     1741 atgaacgcgc ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa agtgatgagc
     1801 attctggatg caggcgaaac cggtaaatac ggtcacccga cgccaaccca ggtcaacgtc
     1861 aaagcgacgg cgggtaaatg cattctgatt tccggtcacg atctcaaaga tctctacaac
     1921 ctgctggaac agaccgaagg cacgggcgtt aatgtctaca cccacggcga aatgctgcct
     1981 gcgcatggct acccggagct gcgtaaattc aagcatctgg tcggtaacta cggcagcggc
     2041 tggcagaatc agcaagtgga gttcgctcgt ttccctggcc ccatcgtgat gacctcgaac
     2101 tgcatcatcg acccaaccgt aggcgcttat gacgatcgta tctggacccg cagcatcgtt
     2161 ggctggcctg gcgtgcgtca tctggatggc gaagatttct ctgcggtcat cgcccaggcg
     2221 caacaaatgg cgggcttccc gtacagcgaa attcctcacc tgatcaccgt gggctttggt
     2281 cgccagacgc tgcttggtgc agccgatacg ctgattgatc tggttagccg tgaaaaactg
     2341 cgtcatatct tcctgcttgg tggctgtgac ggcgcacgcg gcgagcgcca ctacttcacc
     2401 gatttcgcca ccagcgtgcc ggatgactgc ctgatcctga ccctcgcctg tggtaaatat
     2461 cgctttaaca aacttgagtt tggcgatatc gaaggtctgc cgcgtctggt agatgcaggt
     2521 caatgtaacg atgcttactc agcaattatt ctggctgtca ctctggcaga gaaactgggc
     2581 tgtggtgtga acgatctgcc gctgtcgctg gtgctctcct ggtttgaaca gaaagcgatt
     2641 gtcattctgc tgacgctgct ttctctgggc gtgaaaaata tcgtcaccgg cccgactgca
     2701 cctggtttcc tgacaccaga cctgctggcg gtgctgaacg agaaattcgg cctgcgttct
     2761 atcaccactg ttgaagaaga catgaagcaa ctgttgaacg cgtaaggagt ttgtcgatga
     2821 cgatgccaac gaatcaatgc ccgtggcgga tacaggttca tcacattacg caagaaacgc
     2881 cggatgtgtg gacgatttcc ctgatttgcc acgatcacta cccatatcgc gccgggcaat
     2941 atgcactggt cagcgtgcga aactcagcgg aaacgctgcg tgcttacacc atttcctcca
     3001 cgccaggcgt gagtgaatat atcaccctga ccgtgcggcg gattgatgac ggtgtcggct
     3061 cccagtggct gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg gacgcgatgg
     3121 gggaatttac ctgcgacgat aaagcagaag ataaattcct gttgctggcg gcaggctgcg
     3181 gcgtcacgcc gattatgtcg atgcgtcgct ggcttgcgaa gaaccgtcca caggccgatg
     3241 tgcgggtgat ctacaacgtg cgtacgccgc aggatgttat tttcgccgat gagtggcgta
     3301 actatccggt aacgctggtg gcagaaaata acgttaccga aggctttatc gctggtcgcc
     3361 tcactcgcga actgctggca gatgtacctg acttagcttc acgtaccgtg atgacctgcg
     3421 gcccggctcc gtatatggat tgggtagagc aggaagtgaa agcgctcggc gtgacgcgtt
     3481 tctttaaaga gaaattcttc accccagtag cagaagcggc gaccagcggt ctgaaattca
     3541 ccaaactgca accggcacga gaattttacg ccccggttgg caccacgcta ctggaggcgc
     3601 tggaaagcaa taacgttccg gttgtcgctg cctgccgtgc gggtgtttgc ggctgctgta
     3661 agacaaaagt ggtttccggt gaatatacgg tgagcagtac aatgacgctg accgacgccg
     3721 aaatcgctga aggttacgta ctggcctgct cctgccatcc gcagggggat ttggttctcg
     3781 cataatcgcc ttatgcccga tgatattcct ttcatcgggc tatttaaccg ttagtgcctc
     3841 ctttctctcc catcccttcc ccctccgtca gatgaactaa acttgttacc gttatcacat
     3901 tcaggagatg gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa acactcgaat
     3961 cggcaggcgt gaaacgcatc tggggagtca cgggcgactc tctgaacggt cttagcgaca
     4021 gtcttaatcg catgggcacc atcgagtgga tgtccacccg ccatgaagaa gtggcggcgt
     4081 ttgccgctgg cgctgaagca caactttcag gagaactggc ggtctgtgcc ggatcgtgcg
     4141 gcccaggcaa cctgcacttg atcaacggcc tattcgattg ccaccgcaat aacgttccgg
     4201 tactggcgat tgccgctcat attccctcca gcgaaattgg cagcggctat ttccaggaaa
     4261 cccacccaca agagctattc cgcgaatgta gtcactattg cgagctggtt tccagcccgg
     4321 agcagatccc acaagtgctg gcaattgcta tgcgcaaagc ggtgcttaac cgtggcgttt
     4381 ccgttgttgt attaccgggc gacgtggcgt taaaacctgc gccagaaggg gcaaccacgc
     4441 actggtatca tgcgccacag ccagtcgtga tgccggaaga agaagagtta cgcaaactgg
     4501 cgcaactgct gcgttattcc agcaatatcg ccctgatgtg tggcagcggc tgcgcggggg
     4561 cgcataaaga gttagttgag tttgccggga aaattaaagc acctattgtt catgccctac
     4621 gcggtaaaga gcatgtcgaa tacgataatc cgtatgatgt tggaatgacc gggttaatcg
     4681 gattctcgtc aggtttccat accatgatga acgccgacac gttagtgcta ctcggtactc
     4741 agttccccta ccgtgcgttc tacccgaccg atgccaaaat tattcagatt gatattaatc
     4801 cagccagcat cggcgcgcat agcaaggtag atatggcgct ggtcggcgat atcaaatcaa
     4861 ccctgcgcgc attgctgcca ctggtggaag aaaaaaccga tcgcaagttt ctggataaag
     4921 cgctggaaga ttaccgcgat gcccgcaaag ggctggacga tttagctaaa ccgagcgaga
     4981 aagccattca cccgcaatat ctggcgcagc aaattagtca ttttgccgcc gatgacgcca
     5041 tctttacctg tgacgtcggc acgccaacgg tatgggcggc acgttatctg aaaatgaacg
     5101 gcaagcgtcg cctgttaggt tcgtttaacc acggttcgat ggctaacgcc atgccgcagg
     5161 cgttgggtgc gcaggcaacc gagccggaac gtcaggtggt cgccatgtgc ggcgatggcg
     5221 gttttagcat gttgatgggc gatttcctct cagtagtgca gatgaaactg ccggtgaaaa
     5281 ttatcgtctt taataacagc gtgctgggct ttgtggcgat ggagatgaag gccggaggtt
     5341 acctgacaga cggtactgag ctgcacgaca ccaactttgc ccgcattgcc gaagcgtgcg
     5401 gcattacggg tatccgtgta gaaaaagcct ctgaagttga tgaagccctg caacgcgcct
     5461 tctctatcga cggtccggtg ttggtggatg tggtggtcgc caaagaagaa ttagccattc
     5521 caccgcagat caaactcgaa caggccaaag gttttagcct gtatatgttg cgcgcaatta
     5581 tcagcggacg cggcgatgaa gtgatcgaac tggcgaaaac gaactggcta aggtaaaaag
     5641 ggtggcattt cccgtcataa taaggacatg ccatgattga tttacgcagt gataccgtta
     5701 cccgaccgag ccgcgccatg ctcgaagcga tgatggccgc cccggttggg gacgacgttt
     5761 acggagacga ccctaccgtt aatgctctgc aggactatgc agcagagctt tccggtaaag
     5821 aagccgccat ttttctgcct accggcactc aggccaacct ggtcgctctg ctcagtcact
     5881 gcgaacgtgg cgaagagtat attgtcggtc aggccgcgca taactatctg tttgaagccg
     5941 gtggcgcagc ggtgctgggc agtattcagc cgcaacccat cgacgcggct gccgacggca
     6001 cgctaccgct ggataaagtg gcgatgaaaa tcaaacccga cgatatccat ttcgcccgca
     6061 ccaaattact cagtctggaa aacacccaca acggcaaagt gctgccgcgt gaatacctga
     6121 aagaagcatg ggaatttacc cgcgagcgca atctggcgct gcatgttgac ggtgcgcgca
     6181 tctttaatgc cgtggtggct tacggctgcg aactgaaaga gatcacgcaa tattgtgatt
     6241 cgttcaccat ttgcctgtcg aaaggtcttg ggacgccagt cggttcatta ctcgtcggta
     6301 atcgtgatta cattaaacgt gccatccgct ggcggaaaat ggccggtggc gggatgcgtc
     6361 agtccgggat tctggctgcc gccgggatgt atgccctgga aaataacgtc gcacggttgc
     6421 aggaagatca cgacaacgcc gcctggatgg cggagcaact gcgtgaagca ggcgcggatg
     6481 tgatgcgtca ggacactaat atgctgtttg ttcgcgtcgg ggaagaaaat gctgccgcgt
     6541 taggcgaata catgaaagcg agaaacgtgc tgattaacgc ctcgccgatt gtccgcctgg
     6601 tgacgcatct tgacgtctcg cgcgaacaac tggcggaagt cgccgcccac tggcgcgcat
     6661 tcctggcgcg ttaaggagag agacgtgccg caacgcattt tagttctcgg tgccagtggc
     6721 tacattggtc agcatctggt gcgcacactc agccagcaag ggcatcagat cctagcggcg
     6781 gcacgtcatg tcgacaggct tgcaaagctg caactggcaa acgtcagttg ccataaagtc
     6841 gatctcagct ggccggataa ccttccggcc ctgttgcagg atatcgatac cgtctatttt
     6901 ctggtgcaca gcatgggcga aggcggcgat tttatcgctc aggagcgcca gctggctctc
     6961 aacgtccgcg atgcgctacg tgaagtacca gttaagcaat taatctttct cagttcgttg
     7021 caggccccgc cacatgagca gtcggatcat ctgcgagctc gtcaggctac ggcggacatt
     7081 cttcgtgaag cgaatgtacc agtgaccgaa cttcgggccg gaattatcgt tggcgcaggt
     7141 tcagcggcgt tcgaagtcat gcgcgatatg gtctacaacc tgccagtgtt aacgccgcca
     7201 cgctgggtac gttcacgcac cacgcccatc gcgctggaaa acttgctgca ctatctggtg
     7261 gcgctgttag accatccagc caacgaacac cgcatcttcg aagccgccgg accagaggtg
     7321 ctcagttatc agcaacagtt tgaacatttt atggcggtga gcggtaagcg ccgctggttg
     7381 atccccatcc ccctccccac ccgctggatt tcggtgtggt ttctcaatgt gattacttcc
     7441 gtaccgccca ccaccgccag ggcgttgatt caggggctga aacacgatct gctggcagat
     7501 gataccgcgc tacgtgcact catcccacaa cggctgatcg ctttcgatga cgcggtacgt
     7561 agcacgttga aagaggaaga aaaactggtc aactccagcg actggggcta cgacgctcag
     7621 gcctttgccc gctggcgacc ggagtacggt tatttcgcca aacaggcggg gtttaccgtt
     7681 aaaacgtccg ccagccttgc ggctttatgg ctggtggtga accaaatcgg cggtaaagag
     7741 cgttatttct ttggcaatat tttgtggcag acacgggcgt tgatggaccg cgcgatcggt
     7801 cataatctgg cgaaaggccg cccggagcgc gaatatttac agaccggcga tgcggtggat
     7861 agctggaaag tgattgtcgt tgaaccgaaa aagcaactta cgttgttatt tggcatgaaa
     7921 gcgccggggc tgggacgact gtgttttacc ctggaagata aaggtgacta tcgtactatc
     7981 gatgtccgcg ctttctggca tccgcacggt atgccggggc tgttttactg gttattgatg
     8041 atccccgcgc atctgtttat ttttcgcgga atggcaaaac aaatcgccag actggcagaa
     8101 caaagcacag attaatcgac aaaacctgcg ttattctttc atgtttccca ttgcatcatg
     8161 ggggaaatca cggaagaatg cgcacgtcat tttctcaaca acagtggatt gatatgaagg
     8221 tactggttac cggcgccacc agcggcttag gtcgaaacgc ggtagagttt ttatgccaga
     8281 aaggcatcag cgtgcgtgcg agcggtcgca acgaagcaat gggtaaattg ctggaaaaaa
     8341 tgggcgcaga gtttgttccg acggatctga ccgagctggt ttcatcacaa gctaaagtga
     8401 tgctcgcggg cattgatacg ctgtggcact gctccagctt tacctcaccc tgggggacac
     8461 aacaggcttt cgatctggct aacgttcgcg ccactcgccg cctgggtgaa tgggctgtcg
     8521 cctggggtgt gcgtaacttt attcatatct cttccccctc cctgtacttc gattatcacc
     8581 accatcgcaa tattaaagaa gattttcgcc ctcaccgctt cgccaacgag tttgcccgca
     8641 gcaaagcagc cagcgaagaa gtgatcaata tgctttcgca ggcgaatcca caaacgcgct
     8701 ttactattct gcgcccacaa agtctgttcg gaccgcacga taaagtcttt attccccgtc
     8761 tggcgcatat gatgcaccac tacggcagta ttctgttacc gcatggcggc agtgcgctgg
     8821 tcgatatgac ctactatgaa aatgccgtgc acgcaatgtg gctggcaagc caggaagcct
     8881 gcgataagct accttccggg cgtgtgtaca acatcaccaa cggcgagcat cgcacactgc
     8941 gcagcatcgt gcagaagctg atcgacgagt tgaatattga ctgtcgtatt cgttccgtcc
     9001 cttacccgat gctggatatg atcgcccgca gcatggagcg tttaggccgc aagtcagcaa
     9061 aagagccgcc gctgacccac tacggcgcct ccaagttgaa ttttgacttt acgctggata
     9121 ttacgcgtgc gcaggaagag ttaggttatc agccggtcat caccctggat gaaggcatcg
     9181 agaaaactgc cgcctggcta cgcgaccacg gaaaactgcc gcgctaatcc tgcccgtatt
     9241 tctccagcaa tgcttcggca atcgcctgag tttctgcatc cgcttcgccg ttatataacg
     9301 tcgggcggaa atgcatctgg aatgccataa tcacgcgccg ctgctcgcgc ggtgtcatat
     9361 caggttttac gtcataaccg tagcgcgcca aaagctccag caatgacgcg gtatctaccg
     9421 gtgtgtgcgg cgcgcgcccg gcaaggtaaa agttcacccg ctgcgcatcc ggccaggcac
     9481 caatcccctg ctgcgccagt tgctgccagg gaaataatgg ccccggatcg tctttgcgct
     9541 gcggtgcgat atccgcatgt gccactacgt tttccggctt gatgtgataa cgggcaataa
     9601 tatctttcgc cagcggaata agcgcctgaa tctgtgcctg ttcaaacggg gcaaaatatt
     9661 tcactccgga tgatttttgc cagccacggt tttccagctc aatgccaata gaggtgtcgt
     9721 taaggcgcgt tgccccgcgc caggcgctaa tccccgcatg ccaggccagt tcttgttccg
     9781 gcaccagttg ccagatgcgc ggtttaccgt tgtatcgcgg tggcaccgca gggatcagat
     9841 aatgcgagct gacctgctta tcagtcagtg tcgccagcga gctatcaaaa tcatctgcgg
     9901 tgtagtggat caccagcact ttaatgcgcg gatacgccgc ctgcgcctgg tgtcgggtat
     9961 caagctgata tccctctttc tcgacaatgc ctttttcgcc tgcacaccct gccagtaaca
    10021 gagcggcagc gaccagccag aagactcttc tcatcgacgc gttttcaccg ccgtaccgct
    10081 gacgctaacc atcagcatac tgccgttttg cccgaccgtt tcgtagtcga tatcaatacc
    10141 aactacggca tcggccccca gcgcccgcgc ctgggagcct aattcctcaa aggcgatctc
    10201 ccgtgcttta cgcagttctt tttcatacgc accggaacgt ccgccaacga tatcgcggat
    10261 accggcaaag aaatcacgga aaatattggc acctaaaatc gcttcgccgg tcaccacacc
    10321 gcaatattca acgatggtca ggccttccag agttggggtt gtagaaaatt gcatgatcga
    10381 tctccttgtt tgttaagcct tcgatctcta aagcattatc ggactgatat gccatgttgg
    10441 gaagaaggtt ttgatcagtt taggaaatca tctcattgtg tcaaaaacac ccacttaagt
    10501 ctttacgatt atccgttgca taacccgata ttggtttcat tatagggtac cgcttacctg
    10561 tcttattatg ttgcaccaac aacattaatg agcggaaaga ggcaacttat attgtaaatg
    10621 actcctaaag tcttccccat cttgtgactg aggtctatac cgctggattg ctttttggtc
    10681 agccgccgca aaacaagaaa gtaataagat ctcaatgctt ttgtcattaa tataagcgaa
    10741 gatcattctt gcaaactcgt atataaacgc ccgatcctga gcgcttaata gtttaataca
    10801 atatttattt ttaagagaga aaataatttc aaggaaagca tccgcagcaa ccttcacgtt
    10861 attcccggcg ttcatattat atatatccct tgcacagata gagtacagag atagtaaaca
    10921 gccatacaaa taatcactta ttaattcttt tgtccatttt acaggcaacc agggttttat
    10981 cttgacacca tgcatactac attcatgaaa aagacatttg ctgagattaa ctcttatcat
    11041 atcaaca
//
LOCUS       DAZPEU010000127        10877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPEU010000127 DAZPEU010000000
VERSION     DAZPEU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.128"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..153
                     /locus_tag="ACHU2L_002902"
     CDS             <1..153
                     /locus_tag="ACHU2L_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357673.1"
                     /translation="RKKKNSSSSSSSSSSSSSSRNNNIISNRQMTGLKRQKPPSERPR
                     NERGGA"
     gene            complement(239..802)
                     /locus_tag="ACHU2L_002903"
     CDS             complement(239..802)
                     /locus_tag="ACHU2L_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR1357674.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVSPAPICDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQSVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(849..2210)
                     /locus_tag="ACHU2L_002904"
     CDS             complement(849..2210)
                     /locus_tag="ACHU2L_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR1357675.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPRQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(2262..2492)
                     /locus_tag="ACHU2L_002905"
     CDS             complement(2262..2492)
                     /locus_tag="ACHU2L_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357676.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            3009..3257
                     /locus_tag="ACHU2L_002906"
     CDS             3009..3257
                     /locus_tag="ACHU2L_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357677.1"
                     /translation="MSRGPPQRRRARARNGVAPYIPAAFCRDAGSAGHGATEAARPER
                     SLQARGFHASDRSGSPKPRQRVRRRLAGAPAAACRSRA"
     gene            complement(3530..3721)
                     /locus_tag="ACHU2L_002907"
     CDS             complement(3530..3721)
                     /locus_tag="ACHU2L_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357678.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(3718..4140)
                     /locus_tag="ACHU2L_002908"
     CDS             complement(3718..4140)
                     /locus_tag="ACHU2L_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR1357679.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(4187..4612)
                     /locus_tag="ACHU2L_002909"
     CDS             complement(4187..4612)
                     /locus_tag="ACHU2L_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR1357680.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(5030..5806)
                     /locus_tag="ACHU2L_002910"
     CDS             complement(5030..5806)
                     /locus_tag="ACHU2L_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357681.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(5857..6291)
                     /locus_tag="ACHU2L_002911"
     CDS             complement(5857..6291)
                     /locus_tag="ACHU2L_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR1357682.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(6305..6526)
                     /locus_tag="ACHU2L_002912"
     CDS             complement(6305..6526)
                     /locus_tag="ACHU2L_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357683.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(6527..7210)
                     /locus_tag="ACHU2L_002913"
     CDS             complement(6527..7210)
                     /locus_tag="ACHU2L_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR1357684.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(7287..7580)
                     /locus_tag="ACHU2L_002914"
     CDS             complement(7287..7580)
                     /locus_tag="ACHU2L_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357685.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            7728..8690
                     /gene="parM"
                     /locus_tag="ACHU2L_002915"
                     /gene_synonym="stbA"
     CDS             7728..8690
                     /gene="parM"
                     /locus_tag="ACHU2L_002915"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR1357686.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            8693..9043
                     /locus_tag="ACHU2L_002916"
     CDS             8693..9043
                     /locus_tag="ACHU2L_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1357687.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(9166..9447)
                     /locus_tag="ACHU2L_002917"
     CDS             complement(9166..9447)
                     /locus_tag="ACHU2L_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357688.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(9575..9809)
                     /locus_tag="ACHU2L_002918"
                     /pseudo
     CDS             complement(9575..9809)
                     /locus_tag="ACHU2L_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT         2615 a         2978 c         2740 g         2544 t
ORIGIN      
        1 agaaaaaaga agaacagtag cagtagcagt agcagtagca gtagcagtag cagtagcagg
       61 aacaacaaca tcatcagtaa cagacaaatg acaggtctga aaagacagaa gcccccatca
      121 gaacgtccgc gtaatgaaag gggcggcgcg tgagcgcggc ccctttcagc gccaccggcg
      181 cgcggttttt tcccggcgcg gacagacggg catttatcct cccgccagcc ctgtgtcatc
      241 aggcacgcaa acgaataatc atcgtcggga catcggtata agcaaacgtg ccgcgcggca
      301 gctcctcgcg gacgtcagaa aacggtaaca gcttctcctg ctggcgtggc ccgttgagac
      361 agacggcaac cagaacgccc cccggacgca acagggaaaa agcccgcagg atatgccgga
      421 tatcctgccc gtgactgaac ggcggattca taatgatccg gctgtaatac tgcaccgact
      481 gccattccat gaagtcgcca cactgtaccc tgacaccgtt aaaattttcc cgcagatacc
      541 ggaccagccc gctgttaatc tccaccgcat cacacatggc ctccggcgcg gtatcccgaa
      601 tcgcccgcag tatggccccg gtgccggcag agggttccag aatgtgatca cggttgctga
      661 tttccgccag cgtcaccagg cgatcacaga tcggggcagg actcacaaaa agctgagatt
      721 cagcccggat ttcagcaacc ggcgcggcgt tatccagcct tgcgcgcatc agccggatac
      781 gttctcttaa ctgactgtgc atatcattat ctccgtcatc ccccggggat ttcccggggg
      841 gatatacctt atttctgtgg gatttccgtg attttcatgt cggtgatata cacattcacc
      901 agacggaaat tattgtccat tgtgcggcgg tagcggtatg ccccgtggtc ttctgcttct
      961 tccacactgc gcacggcctt acagtcccgg ggcagggcgg cccactgcgc ctttgtcatt
     1021 tcccggaaac cttcccccgg atagttgact accggcggac gcttcgcggc ctgactggcg
     1081 acggcagcct cttctgccga tggtgctttg tagtccgtga tgcgatcggg cgtcactttc
     1141 atcgtgccgc tgtacccgag aaaactgtaa tttggcgttg tgactgaact caccgccccg
     1201 ttgcttttgt tcacgcggat gatagtcagc cactcgcccc ggctgaaaac ctgtccgccc
     1261 ggctcaaaat cctgtgtccg ggtaaccacg ccgccgcttt cgtccagcat ggcacgctca
     1321 tagatcaggc ggttctgata gtggttaacc cagcgttgct gatgctgaat ctgccgttca
     1381 tgacagcgga tcgcaatttc gcgggcctgc tccgtggtga tgatgtcatc atccagggcc
     1441 gaccataacg accggctccc ttcatactgg cttttttctg ccgggcgcgg atacgtatcc
     1501 agcgggaaac aggcgctgat atggtcatga ctgctgatga gttttgccat attcagatcc
     1561 agcgattcag cccgccacat cgtcagatat ttctgcgact gtgcgatggt cttttcagcc
     1621 ttgcgcaaat cagcttcgat ttttttgata cgacgccagc gaacgtccgg gcgttcttta
     1681 tacttcgcgt gaagcagtgc cgaccgcgcc cgttcttccc agtattccgc acgttcaaag
     1741 agcatcacgg cacgtttcat gccgttttca atacgctgcg catcacgacg ggcgcggcgt
     1801 tcgctgtgat gccccacaag aatgggctga ccgggcggga tcatcgcggc cagtctttcc
     1861 acttcatcaa gtgcctgtgc ggattcactg gcccgctttc cgctgtatcc ggtaaaccgc
     1921 tccgcccgtg cttcctgacg ttcagcgagc gtgctgtctt catcctcaat ctctccggca
     1981 agtgagagga gcacgtcttc ccggcccggt gtccaggctg gcgcgacaaa cagcgcctgt
     2041 ctgggtgccc agcggaaacc ggcatcatgc acttttttat acgtctccgg gtcaagacgt
     2101 gacgcggcat acaggcgcag tttgttgtca tccggtgagt aggtcgcgcg gtacatctgc
     2161 acagggctgg cggcttccgg ggcggtcatg gtgattgcag ctaatgtcat aaaatgttct
     2221 ccttccgtct gttgccgccc ctcgcggggc ggctcacctc atcagttaaa agcgcgtttc
     2281 agttcgagtg tgcggagcac ctgcccgaaa gccgtatttt tcatggcggg gatgtgtaca
     2341 ccacgtcccc ggtgaaaact ccgggcaatg tgtcgcccga aggctttcag cacggggtca
     2401 atttccggcg cggggatgtc ttcgcgtctg aaacggggat gacggcgggg ctttacagcc
     2461 gctgcggaag gtgaaacaat acttaccgtc ataaatgaaa ctccttatga ctgttgaatg
     2521 gaaccgcaga aactgctcga acagtttttg cggtgtggat taagcaggtc ctccccggcc
     2581 tgcttttttc atatctgcgt ctgacgttct ccggtgtttt cctccttctg ttcctgcgtt
     2641 ctcattacct gccagccgga tgactgacag aacaaataaa tgcgtattgc cggagcctga
     2701 aataaatcct gatttttaaa gaacatcacc cgttgagttt cgggagccgc ggttttgacg
     2761 tttcgcggga acctgctgct gtattccctg tggtatgaac ttcacccgtt tgagaacctt
     2821 cgccgcaagg ggcatcactg ccacggcctg acgcacctgt cctgggcaga acgcaaaatt
     2881 ttttaccggt ttttgggtaa aaaattttgt gggctgttca tttaccctgg acagtcagtg
     2941 cggcaggttg tggctgtcta tgcccccggc gacagggact catacgggtg tgtggcaccc
     3001 ggcaggcaat gagcaggggc ccgccgcaac gacggagagc gcgtgcgcga aacggcgttg
     3061 ccccttacat cccggcggcc ttttgccggg atgccgggag cgcaggccac ggcgcgacag
     3121 aggcggcgcg accggaacgg tcgctgcaag ccaggggttt tcacgcatcg gaccgtagcg
     3181 gcagcccgaa accgcgccag cgggttcggc ggagactggc aggtgcgcca gcagctgcct
     3241 gtcggagtcg ggcctgacga ccggatgccg ccaggcagcc gggagccgaa ggtgcgcccg
     3301 gcagacagaa caacactgca gtagtcagaa tcagagcagt agataaagga acaacaacat
     3361 caggaacagt tccataacat aagaaagaaa cactcctgag tcagacacat cacagacggg
     3421 aagggtaacg ggcgacgcgc cagcgtggcc cgcttccctt ccggcaggcg tcagcctgcc
     3481 aggggttttc gcgcgggctg acaggcggcc tccaccgcct gcccctgtat cacctgttta
     3541 tgctgccctg aaggatgtag tgaccgccga ggaatacagc ctgctgattc gccacaaaat
     3601 cgcagatttc atcaacaatc tgcgcagtgg tgagcggttt ctgccccatt tcacgacgcc
     3661 gcgcattaat cacggctttg taattgcgca gaatttcgcg ggtttctgtg ctgatattca
     3721 tgacatgtgc tccccgtcca gcgcctgtaa cacaacgtcc attgcttcgc gttcttccct
     3781 gacgaatgcg tcaccgtcgc ccccgttctc gctcccgacg gcatacaggc gcttcatttc
     3841 actgcctgcc agcgccatca ctttttccag catcaccgcc ggaactgtca cctgacggct
     3901 ggcgcgcttt tcttccatca gctcacgaac aacgtcgtga acaatgctga catccgcacc
     3961 atgcgcaaag gcatcgtcca gtcgctgcat gacggtttcc agctcatcgt ctgtcagact
     4021 ccagtcctgg gctatgtgac gcacatcccc acgggtcagc accacggcaa aaacgcattc
     4081 actgtccggc acgtctgtgt tcagcacttc gcggataatt tctttaacag tacagtacat
     4141 gttctcgtct cctttacagg ggcggcattg ccgccccgtc cgttaatcag tccagtgccg
     4201 cgtaaatcgc gttgcattcg gggtgaaatt cgatgtgacg ccacaactgc gcatcgcgca
     4261 tccggtaaag ctgggtcagt cctgcatccc cgctgtcgtg gtacagccac aactggcggt
     4321 taatcacaag ggaagtcaga atgatgcctg cggcatcagc actcacggta cggtcaaacc
     4381 agtttgcacc gttcaccatg tggaaacggt cgccatccgg catcatgtaa ccgccaccgt
     4441 ccggcaggcg gatgaagtgc cagaagcctc cctgatattc cggcatcatt ttttgcgcca
     4501 gcgcgtacac atacgcctcg gcatacagaa aatcgttgcc aaacagatac ggtagaaagg
     4561 agagacggtc gcactcttca acgttcagag tgacaggttt cgcatattgc atgatgtttt
     4621 cctccgttgt tcacatattc cgcgacaccg ggcggtgccg tgcctccata aaagccgggc
     4681 gataaggcgg ggatgcacag ggcgcaaggg aagccgcagc ctgacgcaca cggctggcgg
     4741 gagttggagg gtgagaccgc gaaatctgag cgggtgaacg accggaaaat cacggcagac
     4801 gggaaggagg gaccgggtga ccgcagcggc gaagcgtacc cttgcatccc tgccagcccg
     4861 gctacgatgg aggcacggca cagcccgcac acgacgctga ggccacataa ctgaaaaccc
     4921 acaaggccat gccccgcatg gccgggtaaa gcggagccgg cggcgacttc ctttgtgctc
     4981 tgtccgggga ccggcaggaa aaaggcgggg atttaccccg ccaacgtcat catttccgga
     5041 tttttgccag ggcatccgcc atgctgcgga tttcggcccg ggcttcccgg accacctccc
     5101 gcagaacacc acggtcaaac cagcggcggg gcggttcatc cggtatacgc agcacatcgc
     5161 aggccagccc actggacagg atatgctcaa tacaatcgct gaccaggtac atttccaccg
     5221 acgaaaaacg tgctccccgg ttcacgatgg catcgagcac cacgggcagg ttttcgtcat
     5281 cttcatcgag ccagcagggg tgatcgcttt ccttcacaac cagacgaaag cggcgcgtct
     5341 ggcgagtcag caacgtctga ccggtggtgg tcagcgccgc ccggatacgc cgcgcatctt
     5401 ctgccacccg gcaactgaca ctgcgtaccg catcgcgcac cgccgcaagt acagccctga
     5461 tttcgtcttc ggtcagccat tctccggcat acgggaaaga cacctgcgtt ccgtctgccg
     5521 gaagtaccgg cagcgactgc cactgactgc cggacagtac cagttccgcc ccggttccgg
     5581 tcagtttctg aatggcggag gccaggagcg gcggcagtac gccgccgtga cggttgataa
     5641 gtaacgtcgc cagccggggc gcatccagca gaacataaat gctgatccag cccggatact
     5701 tcgggttacg ctggtcaaca tccgtctctt ccggccagcc ctgcgccagc agcaggttgc
     5761 gggcgtgacg acaaatcagt gcgttcagcg tgctgttttc gttcatgatt aagcgttctc
     5821 cctgtatcgc cgcccgcagg gcaggcggcg ataacatcag tcgttttcgt gtgtgttgtt
     5881 tttcagcagg gtgcgggcct gattaaccac ctcctgacgg cgggtgacgc attccgggac
     5941 ggcgaggcca tgatcccggg cggcccattc acgtttccac aatgcctgtt cagcggtctg
     6001 aatcagggac gccagcagtt cagcagggac ggtcaccagg cggttttcgc tctcgttgcg
     6061 gataatctcc atcaccgcag cggaagaaat accggactca atccgctggt cttccgggat
     6121 gtcctccagg cgtgccagga cggtcctgat ttcatgaccg gtcagagaaa tttccatacc
     6181 gtcagccagt gcctgaatct cttccgggga ccagataacc agcatcagcg gcatgtctcc
     6241 cggatacttt actgccagct cccggcatag cgtttcgcat gttccgtaca ttctcgtctc
     6301 cttctcaatc tttaaagcac gcttccagtt ccagaatttc ggtatatgcc tccatcagga
     6361 ggcggtttgc gcgaaacagc gtctgacgca ccagacgttc cggcagggtg tcggcattaa
     6421 agctgtaccg ctgctccatc tcattcatgg ttgtacgcag gtcgcacatg gcattggcca
     6481 cctccgccac ttcggcgcgc agtttttcgt gtcctgcata gttcatttac gcagcctccg
     6541 gcataaacca gtcatcattc gcggccccct gctgcatggc ccgttgcacg gcggcaaggc
     6601 gttgctgccc ggcacggtga tactgctcaa gcagctcgat accgatatac cggcgtccgg
     6661 actggagggc ggcgacgcag gttgagccgc tgcctgcaaa cgggtccagc acaattgcgt
     6721 tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt tccgtcgggt
     6781 gatggcgatt gcccgaatat ttccagccca gcacgtccgg cagcgggttt tgtggcagac
     6841 gtggacggcc ttttgccagg atgtaggcac attcgtggcg atagcccaca tatgcggcct
     6901 tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc gcatttttcc
     6961 aggcggccat aaagcgatcg acgcggttcc agccatagaa gctcaccatt aacgcgtctt
     7021 ttttgagtac gcggtacatt tcattacagg ctggttgcag ccattcatcg gttttatcgc
     7081 cggcgatggt gcgcccggaa cggtcacgga aaccgacgag atacggcggg tcggtgagga
     7141 tgaaatcgac agcattgccc ggaaacgtgg ccataacacg cacgcagtca cccaggataa
     7201 aacgggacat aacagcactc cttctcttac agagggcacc gggagggcac ccctgtggtg
     7261 cccgttcccc gctgcctgtg ggctgttcac cgcgtccggt aacgggtcag gccgaccgtg
     7321 gaataccgca gccgtcgtct ttttgacggc gaggatatgc cgcgaaagcg gcttgaaggg
     7381 cgttcatcag aaaagggcga cgggctggcg tacactgtca ccaacgccag ccgggagcgg
     7441 taaaagcgcc cgtcccgtgc ccggccggac gcgggacact gaccacaggc agcgttcctg
     7501 ccgcgccgcg tcagccggcg cagtaaaaag aggagcccgg cgacatcttt tgttacccgc
     7561 caaacaaaac ccaaaaacaa cccataccca acccaataaa acaccaaaac aagacaaata
     7621 atcattgatt gatggttgaa atggggtaaa cttgacaaac aaacccactt aaaacccaaa
     7681 acatacccaa acacacacca aaaaaaacac cataaggagt tttataaatg ttggtattca
     7741 ttgatgacgg ttcaacaaac atcaaactac agtggcagga aagcgacgga acaattaaac
     7801 agcacattag cccgaacagc ttcaaacgcg agtgggcagt ctcttttggt gataaaaagg
     7861 tctttaacta cacactgaac ggcgaacagt attcatttga tccaatcagc ccggatgctg
     7921 tagtcacaac caatatcgca tggcaataca gcgacgttaa tgtcgttgca gtgcatcacg
     7981 ccttactgac cagtggtctg ccggtaagcg aagtggatat tgtttgcaca cttcctctga
     8041 cagagtatta cgacagaaat aaccaaccca atacggaaaa tattgagcgt aagaaagcaa
     8101 acttccggaa aaaaattaca ttaaatggcg gggatacatt cacaataaaa gatgtaaaag
     8161 tcatgcctga atctataccg gcaggttatg aagttctaca agaactggat gagttagatt
     8221 ctttattaat tatagatctc gggggcacca cattagatat ttctcaggta atggggaaat
     8281 tatcggggat cagtaaaata tacggagact catctcttgg tgtctctctg gttacatctg
     8341 cagtaaaaga tgccctttct cttgcgagaa caaaaggaag tagctatctt gctgacgata
     8401 taatcattca cagaaaagat aataactatc tgaagcaacg aattaatgat gagaacaaaa
     8461 tatcaatagt caccgaagca atgaatgaag cacttcgtaa acttgagcaa cgtgtattaa
     8521 atacgctcaa tgaattttct ggttatactc atgttatggt tataggcggt ggcgcagaat
     8581 taatatgcga tgcagtaaaa aaacacacac agattcgtga tgaacgtttt ttcaaaacca
     8641 ataactctca atatgattta gttaacggta tgtatctcat aggtaattaa tgatggacaa
     8701 gcgcagaacc attgccttca aactaaatcc agatgtaaat caaacagata aaattgtttg
     8761 tgatacactg gacagtatcc cgcaagggga acgaagccgc cttaaccggg ccgcactgac
     8821 ggcaggtctg gccttataca gacaagatcc ccggacccct ttccttttat gtgagctgct
     8881 gacgaaagaa accacatttt cagatatcgt gaatatattg agatcgctat ttccaaaaga
     8941 gatggccgat tttaattctt caatagtcac tcaatcctct tcacaacaag agcaaaaaag
     9001 tgatgaagag accaaaaaaa atgcgatgaa gctaataaat taattcaatt attattgagt
     9061 tccctttatc cactatcagg ctggataaag ggaactcaat caagttattt tcttaccagt
     9121 cattacataa tcgttattat gaaataatcg tttgcactgt ctctgttatt caggcaattt
     9181 caataaaggc acttgctcac gctctgtcat tttctgaaac tcttcatgct gcatttcgat
     9241 ttttcttcta aaatcataac ttgcatacct gttcttttta tgtatatcca gatctatttt
     9301 tgtgacgagt tcaaaccaca atgatagcac tccccatgca gcgttatgaa acatttccgc
     9361 aacagattta gagtctgtat taaccgcttc agtcatgtag tcgctaatac gttctgcagt
     9421 gattttcgtt atttcatcgt aattcatttt atcccctaca agactattta atggaatgaa
     9481 aaatatttgg tctgataaca atgctcctga attaacacct gcaggctatg ttaaagttgc
     9541 cctcacacac aatctacctg ttttctcgtg tgtgtcacag gcgcaataca taaatagtct
     9601 ggccattaag ttcgtaacgg atttcgtact cgccagcaaa aatccgtctg acatccctgg
     9661 tttcaaactg aaacaactgt tcatcccata cgtggatgag ttaacagaat gaccggcgcc
     9721 tgtgtcaggg actacaccgt tttggctgca gctggtttac tgaccaacgc cagaaaatca
     9781 tataactgcg tcaatgtcag cgtatccagc gcgactgtat tatttttctc tgcaaccaga
     9841 atattgcatt aggttattac caggtgttct aatgtacagg taaagacgtt ctttataatc
     9901 tgaaatctct acttttaatc cctcagactt tgcctgctga aagccctcgt caaggtgcgc
     9961 ttttattagg ttaatgtctg gatgtcttgt atcgggggca attctgtatt ggtgtaacga
    10021 tggggtggat gttccctgca ataagacctc cagatagaga ttggcacaac cattcccatt
    10081 atttctaata gtttattggt aatcacaaac tgtatattta gccattatat tgaatcctgt
    10141 gaagtaaacg gcgacggctc aaaattttat caaacctaag ataattctac catcaaagtt
    10201 aataatttga atttatatga cggctctatg ggggggtaaa aatccccgtg cagagaggcg
    10261 attttcagag ttgaaaacgt cgtcgttaag aacaccaaag tcagtaccac caacccggaa
    10321 agtagcttca tgcaccggga caacagcagt tttggtgtcc gaaatcgtac aattctgtac
    10381 atctacgcaa ggaaatgtac ggaagtaagc caaatggtta tcggacggtc ggaacatttc
    10441 gtacagcctt tacgttcagt gagttagctg gcctagatgt aaccactcgt tcaactcaat
    10501 tctcttagcg tgggaaaatt tccgttttcc aagcggcccc ctcaagcgga ccccttatag
    10561 tacaataatt ttcgatatcc aaactgcccc caattacatc tccagtctct ggcagcagag
    10621 atcatatatt tccattgccg ccgtagccac gatattctgg ctattcacaa aaacaattta
    10681 gccccaaagc gcagtaaaaa agtacactat gaaggtgtat gataccttca tagtgcttaa
    10741 gtaattatga gtaatataat ctgtgtaaac tactaattgc ttcaccgtca gtgattaaat
    10801 gcgtagctgt tccttgtctt atataatacc tattttcaaa atgacagact tctccgacac
    10861 tttttatttc gattttt
//
LOCUS       DAZPEU010000128        10770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPEU010000128 DAZPEU010000000
VERSION     DAZPEU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.129"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHU2L_002919"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHU2L_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR1357689.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHU2L_002920"
     tRNA            complement(621..695)
                     /locus_tag="ACHU2L_002920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHU2L_002921"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHU2L_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHU2L_002922"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHU2L_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR1357690.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /gene="yfdF"
                     /locus_tag="ACHU2L_002923"
     CDS             complement(2932..3990)
                     /gene="yfdF"
                     /locus_tag="ACHU2L_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR1357691.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKKGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="ACHU2L_002924"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="ACHU2L_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR1357692.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="ACHU2L_002925"
     CDS             6068..6352
                     /locus_tag="ACHU2L_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR1357693.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="ACHU2L_002926"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="ACHU2L_002926"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR1357694.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="ACHU2L_002927"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="ACHU2L_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR1357695.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="ACHU2L_002928"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="ACHU2L_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR1357696.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2669 a         2499 c         2700 g         2902 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttt cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc
//
LOCUS       DAZPEU010000129        10668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPEU010000129 DAZPEU010000000
VERSION     DAZPEU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.130"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1812)
                     /gene="selB"
                     /locus_tag="ACHU2L_002929"
     CDS             complement(<1..1812)
                     /gene="selB"
                     /locus_tag="ACHU2L_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR1357697.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHL"
     gene            complement(1809..3200)
                     /gene="selA"
                     /locus_tag="ACHU2L_002930"
     CDS             complement(1809..3200)
                     /gene="selA"
                     /locus_tag="ACHU2L_002930"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR1357698.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3298..3906)
                     /locus_tag="ACHU2L_002931"
     CDS             complement(3298..3906)
                     /locus_tag="ACHU2L_002931"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR1357699.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3977..5062)
                     /locus_tag="ACHU2L_002932"
     CDS             complement(3977..5062)
                     /locus_tag="ACHU2L_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1357700.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5065..5427)
                     /locus_tag="ACHU2L_002933"
     CDS             complement(5065..5427)
                     /locus_tag="ACHU2L_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1357701.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5964..7877
                     /gene="mtlA"
                     /locus_tag="ACHU2L_002934"
     CDS             5964..7877
                     /gene="mtlA"
                     /locus_tag="ACHU2L_002934"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR1357702.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7972..9120
                     /gene="mtlD"
                     /locus_tag="ACHU2L_002935"
     CDS             7972..9120
                     /gene="mtlD"
                     /locus_tag="ACHU2L_002935"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1357703.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9120..9707
                     /gene="mtlR"
                     /locus_tag="ACHU2L_002936"
     CDS             9120..9707
                     /gene="mtlR"
                     /locus_tag="ACHU2L_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR1357704.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9719..9928)
                     /gene="yibT"
                     /locus_tag="ACHU2L_002937"
     CDS             complement(9719..9928)
                     /gene="yibT"
                     /locus_tag="ACHU2L_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR1357705.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10212..10574
                     /locus_tag="ACHU2L_002938"
     CDS             10212..10574
                     /locus_tag="ACHU2L_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR1357706.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2562 a         2882 c         2751 g         2473 t
ORIGIN      
        1 taaatgatca tttccacgac gacgcgtaaa gccaatgcgg tcaaaatatt ccagaatctg
       61 aattgccagc tttcggccta cgcctaagcg atcgcggaaa tccgccgcgc aggttgaacc
      121 acactcctga tcgagatcgc ggatcatatt ggcaaattcg acaatccgat cgttacggta
      181 ataacgatct ttgacgatcg cggtaattat tccttgctgc gccgcctggc gtagcgtcag
      241 gcgcattgcc tgctcgtcgg tacccgtctc ttttgccaga tcacgcaccc accacggctc
      301 gtcaccaaac agcggctctg ctttttgcca gatggcctgc tgctcttcgc tgaagcccgc
      361 tttgtgatcc ggcagatgca gccaaccgtg atgactgagg atgtcgccgc tttcgcgcat
      421 cttttcaatc agcaacagta ctagcgcttc atcttccatt ggcaacgcca tacgtcgcag
      481 acgttcgcgc ccaggcccag gttcatcgcg atgttgctca tgataagtcg ctaatgtgtc
      541 gagaattttc cgttgccagc gagcggcaac cggcgcattc aacaagctat aaccagcctg
      601 aatatagcca ggctgttgca gcagttcgcg catcccttcg ccgttgagct ggcgcgccca
      661 agcgaaatca ggaagattaa ccgcgccgcg ttgcaggtga acggctaacg cttcgctatc
      721 attctgtgcc ctggcgaggg aagccagcca ttgcagatac tctggtttac gtttaccgcg
      781 acgcggcggg ttaagcatca cgacgcgcgc tccggccagc gtgttgcggg cagagatatc
      841 gcgcaatacc aggcggtcgt tatctgccag ccataacggg gtgtcgaaga ccagttcagc
      901 aaggttatct tccagcagtg aaacgcgtcc cgtgacgtgg ctggcggcgt ggtgaatatg
      961 cagcggctgc cactgggtca gcggtgcatg ggtttgaagc tcgacaatca cccgtgtgaa
     1021 cggctctggc ggcacatcgg caagcagcca gtcgccacgg ttaatctgct ctttttccgc
     1081 atcacccgcg atgtcaagcg cgatacgctg cccggcattg gcggtttctg ttggctggtt
     1141 ttgcgcatgc agcgcacgta cacgcatcgg tttatttaca ccagtcagcc agagtgaatc
     1201 gcctaccttc acttccccgc ttaacgccgt accggtgacg accagcccgg caccttttac
     1261 ggtaaatgcg cggtcaatcg cgaggcggaa actatgttgg ctggcgtgct cgcgttccgg
     1321 caactgaagc agatgctcgc gcagggcatc aattccccga ccttcggttg ctgcggtgat
     1381 aaacagtttt gcctcagcaa aaccgtattc ccgcagaacc tcctttacct ggcgttcaac
     1441 ctcatcaaca cgcgcttcgt ccacgcgatc ggctttggtc agcgccactg tcagcatcgg
     1501 gttaccggtc agctgcaaaa tcgccagatg ctcacgggtc tgtgccatca cgccgtcatc
     1561 gcacgccacc accaacagcg cgtgatcgat accaccaacg cccgccagca tgttggaaag
     1621 aaacttttca tgaccgggaa cgtcgataaa accaggcacg cgaccatccg gctgcggcca
     1681 gtaggcatag ccgagatcga tggtcatgcc gcgctttttt tcttccggca gacggtcagc
     1741 atttacgcca gtaatcgcct gcaataaggt tgttttgccg tggtcaacgt gtccggcagt
     1801 cgcaataatc atttcaacaa catctccaaa aaccgttgct catcttcaag gcagcgtaaa
     1861 tccagccaca atcgtccgtc ataaatacga ccaatcaccg gcactggcaa ttcacgccag
     1921 cgggcggcta atgactcaag gtggctaccg cgtccatcat ggggtgtaaa cgttaatgcc
     1981 gcgctcggca ggcgatcaac cggcagcgaa ccactgccaa tctgcgaaag acatggcata
     2041 acctgtaccg caaactccgc gccgtaatgt gcgacaaggg gagcctgtaa acgttgtgcc
     2101 tggatttgaa tgacctctgc gctgcgggta agcaggcgca gggtcggtaa tttttcactc
     2161 agagcttcag ggtgtaaata aagacgcaac gtggcttcca gcgccgcgag ggtcatttta
     2221 tccgcgcgta atgcacgctt cagcgggtgg ctttgcaggc gggcgatcat ctctttttta
     2281 ccaacgataa tcccggcctg cggcccgcct aacaacttgt cgccggagaa gctcaccaga
     2341 ctgacgcccg ccgcaatcaa ctcctgcggc attggctctt tcggcaaacc gtactggcta
     2401 agatcgacca gcgagccact gcctaaatca gtcaccactg gaacatccag ctctttgccg
     2461 agcgccacca gttcagcttc gtctatcgct ttggtgaacc cctgaatgct gtagttactg
     2521 gtatgtactt tcatcaacag tgcggtattt tcattcaccg cctgacgata atcattcgcg
     2581 tgcgtgcggt tggtggtccc tacttcgtgt agggtgcagc ctgcctgacg cataacatcg
     2641 ggaatacgaa acgcgccgcc aatctccacc agttcgccgc gagataccac cacctctttc
     2701 ccgctggcag tggccgccaa catcaataac accgccgccg cattgttatt gacgatacag
     2761 gcatcttccg cccccgtaat acggcacagc agctgcgcca gcgcccgatc gcgatgtccg
     2821 cgtccggcgt cgtccagatc gtactcgagg gtcactggcg aacgcatagc ctgcgcaacg
     2881 gcttccaccg cggcttccgc ctgtaaagct cgcccaaggt tggtatgcag cacggttccc
     2941 gtcaggttga tcaccggacg cagcgcgctc tgcgcctctt tcgtcaaccg ggcatcgact
     3001 tcttgcgccc agttttcaca ccacgcaggc agcgtctggc tgccacgaat cacttctcgc
     3061 gcttcgtcga gcatctgacg caacaattcc accacgcggg tgtgaccata agtatcacgc
     3121 aaagaaagga aggagctatc gcgcaataag cgatcaatag ccggaagttg actatagagg
     3181 gaacgcgttt cggttgtcat aggaaacctg gctgatcaag gccctctcac acggagaagg
     3241 gcgtttaaca taaccacgga ttgtaacgtg agatgggtca ggaggacata tcgcgcatca
     3301 agcctttggc ggttcggtgc gagcaaaact ttcgcggcta aacaggtttt cgaccagttt
     3361 gactaagtgt gggcggtcga cacaccaacc tggcgcaacg cggcggaaat tgagatatcc
     3421 gacagcacag gcaatggcga tagtcgccag attgaccgta tcggttttga gtgtgccatc
     3481 gaccagatat ccttccagca catccagact gcggttgatt ttctcccgct ggcgtaacaa
     3541 ttcatcttca gactgctgcg ccgccggacg cgcctgttca cgcaccgata ccagcccggc
     3601 atccataatg ccatccgcca gcgcctcaat tttgcgcacc cgcaacgact ccagcggatc
     3661 gcgcggcaac atcgccggag cgacattcat taactcaata tattcagcga tgatcggcga
     3721 atcaaaccag cattcgccct cttcggtcac cagcaccggc actttcccta acgggttaaa
     3781 ttgcgccacg ccgttatccg cgttataggg cagttcatta ataaattcga aagttatgcc
     3841 cttttctaac aacagaataa aaagtttgcg tacaaacggg ctggtgtagc taccgacgag
     3901 tttcatgccg agtcctttgt gcgaggaaaa atatcagtat ggctcaggct cacgcaaaac
     3961 acaggtgata taagtctcaa tgatccaaat aaagataatg catccaactg gtcattctta
     4021 gcagcacttt ccgcatcacc gaaacatggc tgaaatggtc ggagtaaacc gccacctggg
     4081 cgtagatgcc gtcgggtaag gcatcgatat catcgttagg gtccagttca atggttccca
     4141 gcacaccgtc cgtgccggga acgaccgtta atgattgcaa taccccctgc gcctgataag
     4201 aaccgcctgg cacgacaggt aaaatactag tcaatttgcc gtggaacacc tgcccaggta
     4261 gcgcgttaaa caccacttcc gcatcatcgc caggtttcag acgtaacagc gagttttgcc
     4321 gaaattgggc gacaatttgc cgtttttgct cggggatgaa gaccatcacc ggacgcagcg
     4381 gcaaggcagc tgcgtatgta cctgggcgga tcagtacctg agtgacgtag ccattgcttg
     4441 gtgcgcgaat gacagtctgc tcaagattat attttgcttc agtaagttgc gctcttaagc
     4501 tcacaatctg cgattgctcg ccgttgacca tactgtcgag ctgactctgg atctgcgcct
     4561 gctccgccac cgagcctttc actaacgcat cctgcgcgag gaaattttgc cgcgcatcgt
     4621 cgatgtcacg ttccgagaac ggattcaccg ccgcctggct gcctttcagg taacgttgat
     4681 aatttttaaa cagacggtcg cgctccgctg aaacctgggt ggtgttggcc tgcgcttcag
     4741 tgagctgcgc acgcagcgtc tttatattat gcgtcgccgt catcaggtca gcctgaagtc
     4801 tgtcaactcg cgcctggtaa cgaaccgggt cgagcttaaa aagcacctcg cccttttgaa
     4861 taagctgatt attcttgtca gtgacttcag taacaattcc cgtcacctgt ggcgtgatag
     4921 ggatcgctat cactgccttt tgcgcggtaa aagtgtaagg gtggttgtag ttcatcaaca
     4981 aaatcaaacc actcaccaga aacacgcctc ccagcgccgc cgtcgccagc gccacgtaag
     5041 ttaaaacaat caatagatcc ataattagat ctccgggaac gtggaatagt cagtcttttt
     5101 ctcggcggcg agttgatgct ccagccggga aattcggtca gaaagtgcgg cgatttccgg
     5161 ttcagtcgct ttctcctgcg acgcaacatg tgactgcata ccccagccac gctccggttg
     5221 atagagcgtc gcccagatcc acagaaacgg ccagataaca tgcagagtaa acaggctcac
     5281 ccagcccgcc gtatgaatag cgtcggcatg ggggtgattg cgctttttgg caatcagata
     5341 agggatgtca tgtatcgcga tgattccata aaaaatcacc agaaagacga agatcagcac
     5401 tcccaacgcg aaatagttta gaaacatatc tgcctcggat tcacgtttat cagtgttgtt
     5461 ttttggctgg cagccagaag ggagtcaggc tgatattttg acaataatcc gggttcgcga
     5521 ttctcgccat aacaccaaag aataattttt agaggtgatg agttgcttag ttacataacg
     5581 attgtatgac gaaggcataa catgctgtag atcacatcag gtgaacgccg taagaaaata
     5641 tcttgtgatt cagatcacaa agattcaaca aaccatcaaa acaaaaatgt gacattactc
     5701 acatttaaat gccattttta gcgaaaatca ccgccttgtt tcttttttat acaggcgttt
     5761 tgtgatgagc gtcacgtcaa ttacctctct accccctata tttatgtgac tgatatcaca
     5821 caaaagcccg tcgactggac agttaaccga ttcagtgcca gatttcgcag tatctacaag
     5881 gtccggctac ctctgccgcc acattaacaa aaaacctcgg gcttccagcc tgcgcgacag
     5941 caaacataag aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg caaagctttg
     6001 gtcgtttcct cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg tggggtatca
     6061 tcaccgcgtt atttattcca acagggtggt taccgaacga gacgctggcg aagctggtcg
     6121 ggccgatgat cacttatctc ctaccgctgc tgatcggtta taccggtggt aagctggtag
     6181 gcggcaaacg tggtggcgta gtcggtgcca ttaccaccat gggcgttatc gtcggcgcag
     6241 acatgccgat gttcctcggt tctatgattg caggtccgct gggcggctgg tgcattaagc
     6301 acttcgaccg ctgggtagac ggtaagatca aatccggttt tgagatgctg gtgaataact
     6361 tctccgcagg catcatcggg atgatcctcg ctattctggc attcctcggc attggcccga
     6421 ttgttgaagc cctgtccaaa atgctggctg cgggcgttaa cttcatggtt gtccatgaca
     6481 tgctgccgct ggcgtctatc tttgttgaac cggcgaaaat cctgttcctc aacaacgcca
     6541 ttaaccacgg tatcttctcg ccgctgggta ttcagcagtc ccatgaactg ggtaaatcaa
     6601 tcttcttcct gattgaagct aacccaggtc caggtatggg cgtgctgctg gcgtacatgt
     6661 tctttggtcg tggtagcgct aaacagtctg cgggcggtgc ggcaatcatc cacttcctgg
     6721 gtggtatcca cgaaatctac ttcccgtatg tgctgatgaa tccgcgtctg atcctcgcag
     6781 tcatcctcgg cggtatgact ggcgtgttca cgctgactat cctgggcggt ggtctggttt
     6841 ctccggcatc tccgggttct atccttgctg tactggcgat gacaccaaaa ggtgcttatt
     6901 tcgctaacat cgcgggtgtg tgtgcggcga tggctgtctc cttcgttgtc tctgctattt
     6961 tgctgaaaac cagcaaagtg aaagaagaag atgatattga agcagcaact cgtcgtatgc
     7021 aggacatgaa agctgagtct aaaggcacat ctccgctatc tgcgggcgat gtgactaacg
     7081 acctgagcca cgtacgtaaa atcatcgttg cctgtgacgc cggtatgggt tcaagtgcga
     7141 tgggcgcagg cgtgctgcgt aagaaaattc aggatgcagg tctgtcgcag atttcggtca
     7201 ctaacagcgc gatcaacaac ctgccgccag atgtggacct cgtcatcact caccgtgacc
     7261 tgaccgaacg cgctatgcgc caggttccgc aggcacagca tatttcgctg accaacttcc
     7321 tcgacagcgg cctgtacacc agcctaaccg aacgtctcgt tgctgcccag cgccatactg
     7381 aaaacgaagt gaaagtgaaa gacagtctga aagacagctt tgacgattcc agtgctaacc
     7441 tgttcaaact gggcgcggag aacatcttcc tcggtcgcaa agcagcaacc aaagaagaag
     7501 cgattcgttt tgctggcgag cagctggtga aaggcggtta cgttgagccg gaatacgttc
     7561 aggcgatgct ggatcgtgaa aaactgaccc cgacttatct gggtgagtct atcgcggtgc
     7621 cacacggtac ggttgaagcg aaagatcgcg tactgaaaac gggcgtggtg ttctgccagt
     7681 acccggaagg cgtgcgcttc ggtgaagaag aagatgacat tgcccgtctg gtgattggta
     7741 ttgcagcccg taacaacgag cacattcagg ttatcaccag cctgaccaat gcactggatg
     7801 atgagtccgt catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg ctggaactgc
     7861 tggcaggtcg taagtaatcc aatcctaccc tctcctcacg gagagggttt gggtgaggga
     7921 aaagcctcac cccagccctc tcgggtaaaa acattgatga aggttaatac tatgaaagca
     7981 ttacattttg gcgcaggtaa tatcggtcgt ggctttatcg gtaaactgct ggcagacgcg
     8041 ggtatccaac tgacgtttgc cgatgtcaat caggtggtac ttgatgccct gaatgcccgt
     8101 catagctatc aggttcatgt ggtcggtgaa accgagcagg tagataccgt ttccggcgtc
     8161 aatgctgtca gcagcattgg tgatgatgtc gttgatctga ttgctcaggt cgatttagtc
     8221 actaccgccg ttggcccggt tgtgctggaa cgtattgctc cggctatcgc caaagggctg
     8281 gtgaaacgca aagaacaagg taatgaatcc ccgctgaaca tcatcgcctg tgaaaacatg
     8341 gtacgcggca ccacgcagct gaaaggtcat gtgatgaacg ccctgccgga agacgccaaa
     8401 gcgtgggtag aagagcacgt tggctttgtc gattccgccg ttgaccgcat cgtaccgcct
     8461 tcggcttcgg caactaacga tccgctggaa gtgacggtag aaactttcag cgaatggatt
     8521 gtcgataaaa cgcagttcaa aggcgcactg ccgaacatcc caggcatgga gttaaccgac
     8581 aacctgatgg catttgtcga acgtaaactc ttcaccctga acacgggtca tgctataacc
     8641 gcgtacctcg gaaaactggc cggtcatcag accattcgtg acgctattct cgacgagaaa
     8701 atccgcgcgg tggtaaaagg tgcgatggaa gaaagtggtg cggtactgat caagcgctac
     8761 ggctttgacg cagacaaaca tgcggcgtac atccagaaaa tcctcggtcg ttttgagaac
     8821 ccgtatctga aagatgatgt agagcgcgta ggccgtcagc cgctgcgtaa actgagtgct
     8881 ggcgaccgtc tgatcaagcc actgctcggt acgctggaat acagccttcc gcacaagaat
     8941 ctgattcagg ggattgctgg tgcaatgcac ttccgcagtg aagatgatcc acaggctcag
     9001 gaactggcag cactgatcgc tgacaaaggt ccgcaggcgg cgctggcaca gatttccggt
     9061 cttgatgcca acagcgaggt tgtatccgag gcggtaaccg cttataaagc aatgcaataa
     9121 tggtggacca ggcgcaggac accctgcgcc cgaataacag attgtcagat atgcaggcaa
     9181 caatggaaca aacccaggcc gttgaaaacc gtgtgcttga gcgtctgaat gctggcaaaa
     9241 ccgtgcgaag ctttctgatc accgccgtcg agcttttgac cgaggcggta aatcttctgg
     9301 tgcttcaggt attccgcaaa gacgattacg cggtgaagta tgctgtagaa ccgttactcg
     9361 acggcgacgg tccgctgggc gatctttctg tgcgtttaaa actcatttac gggttgggcg
     9421 tcattaaccg ccaggaatac gaagatgcgg aactgctgat ggcattgcgt gaagagctaa
     9481 atcacgacgg caacgagtac gcctttaccg acgacgaaat ccttggaccc tttggtgaac
     9541 tgcactgcgt ggcggcgtta ccaccgccgc cacagtttga accagcagac tccagtttgt
     9601 atgcaatgca aattcagcgc tatcaacagg ctgtgcgatc aacaatggtc ctttcactga
     9661 ctgagctgat ttccaaaatc agcttaaaaa aagcctttca aaagtaagca acgtctgctt
     9721 actgcccctc tacctgcttc ggccgataaa gccgacgata atactccaga cgttgtagat
     9781 ataacggcac gctttcatcg ggtattccgg acggaatcgc gttacgggga ggaagttttt
     9841 tcagatactc ccggaacgcc tggcttgatg ccatgaaatc tacggcttta tcgataagaa
     9901 gcggaacgtt ttcacctaat ttgcccatga tgatatctcc gtattacccc gcgccgggaa
     9961 tgcgcggccg ccaattttag tttaggttcc cggtaaagat gacattaagg aaaacgtgct
    10021 gaatcctcaa taatcatccg ctgctgttta ttatcttttt ctatagattt cttaattaat
    10081 caacgaattg cgtagttttt gagcatgtta atgattacgt tgcagaacta ttacaatgtc
    10141 atcagttgtg aacaaagcac ctggtcgcgc atactagggg ctataaattt atctttatca
    10201 gaagccatca catgaaagaa gtcgaaaaaa acgaaatcaa gcgtctcagc gatcgcctgg
    10261 acgccatccg ccaccagcag gccgatctgt cgctggttga agccgcagac aaatatgccg
    10321 agctggaaaa agagaaagcc acgctggaag cagaaattgc tcgcctgcgt gaggttcata
    10381 gccaaaaact gagtaaagaa gcacagaagc tgatgaagat gccgttccag cgcgcaatta
    10441 ccaaaaaaga gcaggctgat atgggcaagc tgaagaaaag tgttcgcgga ctggtggttg
    10501 tgcacccaat gaccgcgctg gggcgcgaaa tgggcctgga agagatgact ggattttcta
    10561 agactacgtt ttaagcccct cagacgactg gttcgatcgt atcaacccgc atctggcttg
    10621 ccttagctgg tcaatgcggg gactctcctg tctactcgcc taattacc
//
LOCUS       DAZPEU010000130        10637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPEU010000130 DAZPEU010000000
VERSION     DAZPEU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.131"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..829
                     /locus_tag="ACHU2L_002939"
     CDS             <1..829
                     /locus_tag="ACHU2L_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR1357707.1"
                     /translation="SMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIYL
                     LPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKIT
                     SALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAG
                     TTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGND
                     PIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(970..1743)
                     /gene="uxuR"
                     /locus_tag="ACHU2L_002940"
     CDS             complement(970..1743)
                     /gene="uxuR"
                     /locus_tag="ACHU2L_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR1357708.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1958..3418)
                     /locus_tag="ACHU2L_002941"
     CDS             complement(1958..3418)
                     /locus_tag="ACHU2L_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR1357709.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3499..4683)
                     /gene="uxuA"
                     /locus_tag="ACHU2L_002942"
     CDS             complement(3499..4683)
                     /gene="uxuA"
                     /locus_tag="ACHU2L_002942"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR1357710.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5023..6366
                     /gene="gntP"
                     /locus_tag="ACHU2L_002943"
     CDS             5023..6366
                     /gene="gntP"
                     /locus_tag="ACHU2L_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR1357711.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6617..7519)
                     /gene="fimH"
                     /locus_tag="ACHU2L_002944"
     CDS             complement(6617..7519)
                     /gene="fimH"
                     /locus_tag="ACHU2L_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1357712.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7539..7982)
                     /locus_tag="ACHU2L_002945"
     CDS             complement(7539..7982)
                     /locus_tag="ACHU2L_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1357713.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8055..8585)
                     /gene="fimF"
                     /locus_tag="ACHU2L_002946"
     CDS             complement(8055..8585)
                     /gene="fimF"
                     /locus_tag="ACHU2L_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR1357714.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8595..>10637)
                     /gene="fimD"
                     /locus_tag="ACHU2L_002947"
     CDS             complement(8595..>10637)
                     /gene="fimD"
                     /locus_tag="ACHU2L_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1357715.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2535 a         2764 c         2636 g         2702 t
ORIGIN      
        1 gtctatgcca ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca
       61 tcatccaaaa gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga
      121 aaatatctat cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt
      181 tgtcgaaatt agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga
      241 tacatgcaga gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa
      301 aatcacctca gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac
      361 agagttagca gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga
      421 ggactataca gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg
      481 aaccacgata ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga
      541 gcagccaaga attgatatct atattgatca gataataact gtctcagggg tagcaaattc
      601 ttccggattc gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat
      661 cattggtata gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt
      721 tatccccggt gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc
      781 agaactgttt gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag
      841 aatgagtact aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt
      901 aatactcatt gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat
      961 tataataagt caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa
     1021 ataaatatcg tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg
     1081 ccacattgcc agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt
     1141 gtgatcgccc aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat
     1201 ccacattgga ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct
     1261 gttatgcgtt gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc
     1321 cggcgcgctg gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat
     1381 atcttcgcgg gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg
     1441 cgcctgtaac agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt
     1501 gttgtggctg cctgagctat caagcacata gataccggca ccccggcgca cttccaccag
     1561 ccctttgatc tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat
     1621 ttctgcaatt tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat
     1681 cagatcgtgg atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt
     1741 catggtgttg tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc
     1801 cttatccggc ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag
     1861 cactggaagt tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag
     1921 tttgattgac cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc
     1981 gcgcaccgcg ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat
     2041 tctgcggcag atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg
     2101 cgccctgata ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa
     2161 tggcattacc ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg
     2221 ccaggtgacg ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct
     2281 gcggtaactt ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt
     2341 tgctgaaacg ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg
     2401 acagcgttgg cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt
     2461 tagtcatggt gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag
     2521 agtggctgcc gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga
     2581 actgtgtgcc cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc
     2641 actgacggaa cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa
     2701 tttcctgtaa ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga
     2761 aggtgacgtt ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca
     2821 gtaccgcgac cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa
     2881 atgctttcag ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg
     2941 acttcggcgc agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt
     3001 gaccgcttgc cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg
     3061 tttgcggacg cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg
     3121 cttctttcat cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg
     3181 tgtacagcag ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca
     3241 ggttaacttc gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca
     3301 gcgcctggtg cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac
     3361 gagaatgatc ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg
     3421 taaactccag aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg
     3481 cagccatgcc ggtgggtatt aacggataaa gaaagcgcgc tggatcgcca gttcgacacc
     3541 gcgaacttcg gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt
     3601 cttcaggtcg tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt
     3661 gccttccgct ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc
     3721 aacgtcaccg ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt
     3781 ggagcgcaga tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc
     3841 gttgtcagca cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt
     3901 tacggtatca accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag
     3961 gatcgggcgc ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac
     4021 tggaataatc gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc
     4081 tttgtacagc tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg
     4141 cagaccggca atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa
     4201 gcgttcagct gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt
     4261 caggatatgc atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc
     4321 gtctggcagc acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta
     4381 gcacacggtg cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat
     4441 ccactgctca tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac
     4501 gacagaccac accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac
     4561 ggaccatact tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc
     4621 ctgacggaca tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc
     4681 catgattttt cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt
     4741 tgtttaggta tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg
     4801 cgtataagtt ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc
     4861 gtcgcaagaa tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg
     4921 ttattgatca gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt
     4981 cgcccaaaat aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt
     5041 ctctgggtgg tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat
     5101 tcaatggtgg ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg
     5161 atgtcgctgc tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc
     5221 atcgtggtgt tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag
     5281 atagcacata cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc
     5341 atcggcctta ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg
     5401 ctggttattg ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca
     5461 gtagcagctg ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg
     5521 gtgaatgctt atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc
     5581 ccaagtgtaa tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca
     5641 acgccatcat tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc
     5701 gtttcgattc tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat
     5761 atctggctgg taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg
     5821 attgcgatgt ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat
     5881 gacatgcagt gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt
     5941 ctgatcatcg gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac
     6001 accatcggca tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc
     6061 actgtgctaa ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg
     6121 attatcagtg ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg
     6181 ctgctggtac tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct
     6241 ttctggttgt tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt
     6301 ctgctggagc tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta
     6361 gcgtaaaaga acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa
     6421 atgtgcccgg acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc
     6481 agtaaagtgc gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa
     6541 gggctaacgt gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca
     6601 atgcgctgta atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc
     6661 ctgcagtcac ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg
     6721 aagttcctac tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg
     6781 tcaactgtac gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt
     6841 tgcccgcatc tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat
     6901 aaacggtaag aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac
     6961 gagcagaaac atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc
     7021 acacaaactg aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa
     7081 ttaatgaacc agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg
     7141 ccaccggcca cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg
     7201 tcggaaatgg atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc
     7261 cgccgtaagc cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt
     7321 tatggcaaaa gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag
     7381 gcgcaaggtt tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg
     7441 cggttttaca ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca
     7501 gggtaatagc tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg
     7561 ctaatcactg cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc
     7621 tgtaacggga agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca
     7681 gtattcaatg tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc
     7741 tggtttttat aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac
     7801 gttcccaccg gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg
     7861 gcagacataa gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa
     7921 accgtacacg gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt
     7981 atcgtcgcac ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc
     8041 atgagcatct ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc
     8101 gcagtgacag gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta
     8161 cccggcgtca gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt
     8221 tgctgctcat tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt
     8281 gcaagcaggt tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa
     8341 acggcattac cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca
     8401 atgttgttaa attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca
     8461 gcggccacac tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca
     8521 gccaaaacgc gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt
     8581 ctcatcacgc ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc
     8641 tctggtggca gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc
     8701 tgaacttttc ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg
     8761 ccgctactct ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg
     8821 tgagttagcg tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc
     8881 gccccacgag tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag
     8941 gtattggtat ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca
     9001 taaccacgcc agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca
     9061 ggcgctttaa caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca
     9121 tgagccaata ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg
     9181 taaccgatat tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt
     9241 ccgctattac catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg
     9301 tcttccagca acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt
     9361 gacatgctgt agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag
     9421 tggctgaaag gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg
     9481 tttttcgtca ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca
     9541 gcctggaatt gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa
     9601 tacagtgttg atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg
     9661 ttataagcga ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt
     9721 gtttcgatgt tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg
     9781 ctggtcgaat aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg
     9841 ttatagagaa aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta
     9901 gcctgcgtca tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa
     9961 ttaaaagcac gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga
    10021 aggccgtgga gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta
    10081 cggtattctc ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact
    10141 gacgaatagg gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc
    10201 aagtcaccac tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc
    10261 gtactattat aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca
    10321 ataccgtgga tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag
    10381 gccaattgtg cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca
    10441 cccaacgtca gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc
    10501 tgccatttat ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg
    10561 tcgcgtaaac gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg
    10621 ctgttacccc caatccg
//
LOCUS       DAZPEU010000131        10557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPEU010000131 DAZPEU010000000
VERSION     DAZPEU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.132"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="ACHU2L_002948"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="ACHU2L_002948"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1357716.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..2537
                     /gene="proA"
                     /locus_tag="ACHU2L_002949"
     CDS             1284..2537
                     /gene="proA"
                     /locus_tag="ACHU2L_002949"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1357717.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2652..2727
                     /locus_tag="ACHU2L_002950"
     tRNA            2652..2727
                     /locus_tag="ACHU2L_002950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Thr,seq:cgt)
     gene            complement(2742..3896)
                     /locus_tag="ACHU2L_002951"
     CDS             complement(2742..3896)
                     /locus_tag="ACHU2L_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1357718.1"
                     /translation="MKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAEAW
                     RVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEKIM
                     EAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMAVE
                     WGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNVTD
                     LEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPAYR
                     SDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALKEM
                     GGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
     gene            complement(3779..4126)
                     /locus_tag="ACHU2L_002952"
     CDS             complement(3779..4126)
                     /locus_tag="ACHU2L_002952"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1357719.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAAKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            complement(4213..4557)
                     /locus_tag="ACHU2L_002953"
     CDS             complement(4213..4557)
                     /locus_tag="ACHU2L_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357720.1"
                     /translation="MSDLAMKVLKWQSTDDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(4658..4837)
                     /locus_tag="ACHU2L_002954"
     CDS             complement(4658..4837)
                     /locus_tag="ACHU2L_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HFR1357721.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKEREGNEKS"
     gene            complement(4934..5863)
                     /locus_tag="ACHU2L_002955"
     CDS             complement(4934..5863)
                     /locus_tag="ACHU2L_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1357722.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVAY
                     IIQDSDARSRGEKGILRYFANISDEDINEYEITVTPLYAAPPETVSVPDAMEMDDDFD
                     SAFEHGKAVGWNAYRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAIQK
                     VAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIAPNDVREQTAIPQ
                     VPVTPDGWISCSERMPVIGELNWRTSFPLLITCEIGVIPAYYGFVSVNGNKHYGFMES
                     LKYGDDNGNHPQTNEYGLISNVTHWMPLPEPPL"
     gene            complement(5866..6057)
                     /locus_tag="ACHU2L_002956"
     CDS             complement(5866..6057)
                     /locus_tag="ACHU2L_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357723.1"
                     /translation="MDGQISIVRPGACDDREIRLIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(6059..6688)
                     /locus_tag="ACHU2L_002957"
     CDS             complement(6059..6688)
                     /locus_tag="ACHU2L_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR1357724.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDLLSEQELKDYGVDI
                     DALNAFKFLAGPETVLALLDERVRNQQYIKSRDQENEDIALTVGKLLIENGRLVADTL
                     RHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIAE
                     LGERGILLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGE"
     gene            complement(6685..7260)
                     /locus_tag="ACHU2L_002958"
     CDS             complement(6685..7260)
                     /locus_tag="ACHU2L_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hNH endonuclease"
                     /protein_id="HFR1357725.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALYEDHQKAIESIKQADAAVKLAHEKFSALAAENAGMKSVIEYCINPDNQPEYHDQGM
                     GCGVEDHGYQRDGYSACYYGWESAMDRVYSEVIPDAIPETPATDTFLDEVRAQGVEMF
                     AASMKVVGGHEHPYSAMANEFAAQLRKGGGQ"
     gene            complement(7257..7421)
                     /locus_tag="ACHU2L_002959"
     CDS             complement(7257..7421)
                     /locus_tag="ACHU2L_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFR1357726.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(7432..7725)
                     /locus_tag="ACHU2L_002960"
     CDS             complement(7432..7725)
                     /locus_tag="ACHU2L_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFR1357727.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEELERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(7749..8132)
                     /locus_tag="ACHU2L_002961"
     CDS             complement(7749..8132)
                     /locus_tag="ACHU2L_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357728.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(8132..8737)
                     /locus_tag="ACHU2L_002962"
     CDS             complement(8132..8737)
                     /locus_tag="ACHU2L_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFR1357729.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDIYNIRRDELEGAAA"
     gene            complement(8839..8997)
                     /locus_tag="ACHU2L_002963"
     CDS             complement(8839..8997)
                     /locus_tag="ACHU2L_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357730.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTETHVH
                     AESSLVSP"
     gene            complement(8994..9146)
                     /gene="kil"
                     /locus_tag="ACHU2L_002964"
     CDS             complement(8994..9146)
                     /gene="kil"
                     /locus_tag="ACHU2L_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFR1357731.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINI
                     PWYRRK"
     gene            complement(9131..9265)
                     /locus_tag="ACHU2L_002965"
     CDS             complement(9131..9265)
                     /locus_tag="ACHU2L_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357732.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLISAAKKQLEALCAMK
                     "
     gene            complement(9460..9930)
                     /locus_tag="ACHU2L_002966"
     CDS             complement(9460..9930)
                     /locus_tag="ACHU2L_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357733.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(9939..10265)
                     /locus_tag="ACHU2L_002967"
     CDS             complement(9939..10265)
                     /locus_tag="ACHU2L_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFR1357734.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
BASE COUNT         2438 a         2634 c         2451 g         3034 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctacgc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaggtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg atccgcgcag aaatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtactaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gtggtggtaa agaaacctgt cgcactaacg
     1741 cggcaacggt ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     1861 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     1921 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1981 cagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     2041 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     2101 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcgctgacac
     2161 agttgcaggc aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt
     2221 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     2281 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     2341 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     2401 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     2461 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     2521 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     2581 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     2641 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     2701 taggttcgac tcctattatc ggcaccattt aaatcaataa gttacctcgc atttaagtaa
     2761 accacgttct cctcttgtgc cgtatttgtg ccattgcgac ttataatcgc atcgattttg
     2821 cttgcgtgct cggtgagatg cccggctgaa aggtgggcgt atctttgaac catttcgaga
     2881 gtttcccatc ctcccatctc tttaagtgca agaagagaga caccggactg aaccagccag
     2941 cttgcccagg tatgcctcag atcatggaag cggaagttgc taatgcctgc ccgctttaac
     3001 gctcccttcc atgccttgtt gctgtcggtt ctcatcttcc ttaccgctgc tgtttttgtt
     3061 ccgtcgcttc ggtaggcagg tttggtgtgg acaaataccc atctcttatg gagcccctgc
     3121 tgttttctta atatctggca tgcggtttcg ttaagaggaa ctccgatcgc attgccagct
     3181 tttgtttcat cagggtgcat ccatgccatt ttcttatcca gatcgacctg tgaccactca
     3241 aggtctgtaa cgttggaacg gcgaaggcct gtcgtgattg caaacatgac cacagggaag
     3301 aagtgaggag caatttctgc aaacaggcgc ttcgattcct cctctgtaag ccatctgatg
     3361 cgtccattct taacgcgtgg tgttgatatt ttgggtgccc tgtcaagcca tccccattca
     3421 acagccatat tgagaatggc gcgaagtatt gccagatgcc tcgtcttcgt tcctttgctt
     3481 gcaagctttg gtttatactc cggcaccgga ttgccaagtc gcaaacacct gtcccggctc
     3541 atctcccagt tcaggcgatg gcggcggttt tccatcccgt ctaccgcctc cattattttt
     3601 tctgttgtta tgtcagagag aatggcttct ctgaagtgca acatccagaa cgatataatg
     3661 ctcttgtcat catcaatgga cttcttatcc gatttctcac gcagccaccg tatgcaggct
     3721 tccttgaata gctttttcgg tgattccccg agatttttta ctctccacgc ttctgctttc
     3781 agacgatcgt gaagttcttg cgcttgcctt ttgtccgatg tttcaagaga gcgtctaact
     3841 cttgatccat ctggcgcgac gaaatcgcag tgccacgtgc caccgcgtag tttgattgac
     3901 atgctttaac ctcctgcaca tcaaccgcat tcaccgcgct attgtgtctc acagacttaa
     3961 gcgccgcaat gcagtctgac ttgcaaatgc gatatgggct tttaggttta tctggattta
     4021 tctttgcggc ctgaagtcgt ccacttcgta tccactgcgt gatagtgcct ttgtctacct
     4081 tcagatacga cgctgcctct tcacgagtga agatttcttc ttccacctgg aatctccatt
     4141 tattggattg gtattattgc ggtaggtctg gatatcattg agcaatgaac aggcctcatc
     4201 gagtgtgagg cgttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt
     4261 gttcccgtac gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag
     4321 gattcctttg aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg
     4381 aatctgctca acaagtgcaa cgcatcgccg gaaatcggct gcgtcatgtg gagcaccgaa
     4441 gctatgacca tagatattct ttttcagtcc acatgcgatt gaggcaagag ttgcgctact
     4501 gatgccgaca tcgtcagtcg attgccattt caaaaccttc atagccaaat ctgacatttc
     4561 ttgtctccaa taaaaaaccg ccatcaggcg gcttggtgtt ctttcaattc ttcaattcga
     4621 atattggtta cattgttttc atatatgaat aaataaatta gcttttttcg ttgccttcgc
     4681 gttctttatt aattttgaca aactcgtttt taccacgctc tccaaatgcg tctttagagt
     4741 cgttgtatcc gcaatcgcag cacacataat caccagacca tccacgcatt gttttttctt
     4801 ttgcaatatt tccagaaccg cattttggac aagacatatc actacctcca aagcatgagt
     4861 gagatgacaa cgtaacattg attggagatt aacaatagat tgctgatgta aaagatatgt
     4921 ataagcttcg ctttcaaagt ggaggctctg gtagcggcat ccagtgggtt acattgctaa
     4981 tcagaccata ttcattagtt tgaggatggt tgccgttatc gtctccgtat ttaagactct
     5041 ccataaaacc ataatgctta ttcccattaa cgctcacaaa gccgtaataa gctggtataa
     5101 cgccgatctc acacgtaatc agcaaaggaa aactagttct ccaatttagc tcgccgatta
     5161 caggcattct ctcactacag cttatccaac catccggagt taccggaact tgcggaatgg
     5221 ctgtctgctc tcgaacgtca ttaggcgcga taggttctgc tgccaactga ctggcatatt
     5281 tgttaatggt aacgataagc tcttgctcag cctcatccag acaatcaccg atacctcgcc
     5341 tgtcaccgtc aaaatcatcg aaatcggcac gaatcttggc aaccttctgg attgcggaca
     5401 acacctcact aggaattacc ggatagttgg ttgacgtttc cgcgatttca cgaaaattat
     5461 tggttgacga attcttgttt tcccgaaagt ttccggactg aagcatggct gcgcgatagg
     5521 cgttccagcc gacagctttt ccgtgttcaa acgcgctgtc aaagtcatca tccatttcca
     5581 tcgcatcagg cacagatacc gtctctggag gggcggcgta aagcggagtt acggtaattt
     5641 cgtactcgtt tatatcctcg tcgctgatat ttgcgaaata gcgaaggatg cctttctctc
     5701 cacgactacg agcgtcagag tcctgaatga tatacgccac aggctctgct tccagcgatg
     5761 ccagcgcaat tcgtgccagt tccatttgtt cgccacgagt aagcccgttt tcaagcggat
     5821 ttttaatgaa caattcaata cgttctttgg taatagtggt catagctatt tcaccttaat
     5881 ctcaatattt cgcagcttta gctctactgg caggtctgac tttcctgtta acgctaatgc
     5941 gagattttct ggagtaatga gagcagttat tgttttcccc atcgccagac gaataatcag
     6001 tcgtatctcg cgatcgtcac atgctcctgg tcgaacaatt gatatttgtc cgtccatctc
     6061 actctccttt gatgcgaatg ccagcggcgc ggattgcagc gatgacctca gaaactttgt
     6121 atgccattac cgtttggtaa tcatcgtgaa aatctgttcg atgaagcatg ctgctacgtt
     6181 ccgggagcag tattccccgc tcccccagtt ctgctatgcg attctctgcg gattccagcg
     6241 ccgcaaccaa ttcgtctaca gttccggcag cttgcagtgc gtaatcggta atagccatct
     6301 catgatcaat ttcagtaccg ttctcattcg ttgaggtgat agcaaaataa tcagagtcga
     6361 tttcgttatc agctaagtgg cgtagcgtat cggcaacaag ccggccgttt tcgattagca
     6421 gcttccctac cgttagcgca atatcctcgt tctcctggtc gcgggatttg atgtattgct
     6481 gattccttac ccgttcatcc agtagtgcca gcacggtttc tggtccggcc agaaatttga
     6541 aggcgttgag cgcatcaata tccacaccgt aatctttaag ttcctgttca cttaacaaat
     6601 catcagctgg caacattaac aggcgttcca ttgctggaat tgcacgttcc gccgcctcac
     6661 gcagtgcctg atagttaatt tcgctcactg gccgcctcct ttgcgaagct gggcggcgaa
     6721 ctcgttagcc atcgctgaat atgggtgctc atgaccgcca acaaccttca tgctggcagc
     6781 aaacatctcc acgccctgcg cccgcacttc atccaggaaa gtgtcggtgg ctggggtttc
     6841 tggaatggcg tctggaataa cctctgagta gacgcgatcc attgccgact cccatccgta
     6901 gtaacaagcc gaatagccgt cgcgctggta gccgtggtct tcaactccac atcccatgcc
     6961 ctggtcatgg tattcaggct gattgtcggg attaatgcag tattcaatga ccgacttcat
     7021 ccccgcattc tccgccgcca gcgccgaaaa cttctcgtgt gccaacttaa cagccgcatc
     7081 agcctgctta attgactcaa tcgctttctg gtggtcttcg tacagagccg aaatcttggc
     7141 ctccgcttca gcaaatttac gcaccagata ttcagcgttt gtttcgttaa cctttaaatc
     7201 acatgggatg catttacctt tcagaaaacc atccatctca attagtgaca tttgtttcat
     7261 ttcttcccac tccgccacat agcattcaga tatttgttgt cattaacaga accgaaactc
     7321 tttctcttaa gtaattcctc tctcgatggc attggcttta cgcgttggcg aataatcatt
     7381 tctgccggaa gaatgccggg attgtatgca agtcctctca tggtaaattc ctcagtcatt
     7441 actgatagcg ccatagcgtg agcggtaatt acgcaggcgc gggtcaattt cagggaagtg
     7501 ggtatatgtg gctttgcgga atggtcggat tgatgtctgg taaattcgct cgagttcttc
     7561 tttctctgca agccatatac agtggcgaaa ttccttttcc tctttcgttt cctgcggtag
     7621 cgacattatc cgatcgtagt tttttctgaa tttatccagc acctccgata cggaattgcc
     7681 ggaacagcgg cgtgggtcat ccgcaccata cagaggcgct ggcataatgg aatccttatg
     7741 ttgctacttt agaagggaat tgaatcgtcg tattcaggat gattttgatg attgctactt
     7801 tgctgctgtt ggctgtttcc tgaagttgca aatccaatct ttgcattcag taattcaaga
     7861 gtgattgatt gtccattttg cccctgataa acatcaaccc tgatgttttc tccggtaatt
     7921 tccacaatgc caccttcaac aagaacacta cggtagtaat ccgcttgcgc tcccggcttg
     7981 gcaaatacaa cggcgctgta gtttgtccat tctttctttt ttgtctggcg atcgtaatac
     8041 tgaacgccag cacggatgtt gaatccgata ttttccccgg cctgaaactc tcttgcgggc
     8101 ttgtttagtc ttacagtaat cgaatgtgcc attaagcagc cgctccttct aattcgtctc
     8161 gtctgatgtt gtaaatgtcc tgcgctttgt gctgctccgg tgtgccttcg agcatcttcc
     8221 acgctttggc gaacgcctgt ttaagctctt ccacggtgtt tttctgcaat gctgcgtcag
     8281 tgaatgcttt tagaacctgt tcaggtgtag gtgatggttt tgattgcttt gctgctgcgt
     8341 tctgctgatg tttatgctcg tcggtatctg catctttcgc atcatcaatg ccgaacaaac
     8401 cattgaggca atacttgcgt gcataagagc ttgtggctcc cgtaacttgt gcagaatcca
     8461 ttcctttctt gctttcttcc tctcgtgcaa gagcggttgc cgtatgactg ttttcgccat
     8521 cggtaatagt tgccgtggct ttcacgtaat accgatcacc aatcaacaca acttcatcgc
     8581 tgattgataa aaacagacca ttcagtaacg gcttaacgcc ttcaagaatg tcttcgcagc
     8641 ttctgtattt atatttaccg aatgagttgt actggttctt tggcgcgttc aagttctcct
     8701 gaatagctgc cagtcttgcg taaaattctt tgctcatatg tttgttctca gaatgggcat
     8761 ggcccaagga aataacgctg atttaatact tcgactcggg acaaattaag gcatacccgc
     8821 attccttcgc ggtcgccatt atggcgatac cagagagctt tctgcgtgta catgcgtctc
     8881 tgtaacttgc tctccttcac tgtggttgca agtgacatga atatctcctt cgttaccgat
     8941 taattctttc atctgacgaa tgaattcttc gtctgaccag ttatctgtaa aactcatttc
     9001 ctgcgatacc acggaatatt gatagctgat ttcatcgctt tatttgcttc aagccacatt
     9061 tttgaatcac caataaatct ggctattact gctttgtttt gtgctgcacg aagcatctgg
     9121 tgattgatgg ctatttcatt gcgcataacg cctccagttg tttctttgct gcactgatta
     9181 attgtttaac tcggcgtgat aattcagatt cgtgcgggta gaaagcggac atgacgccgc
     9241 tacccgcgag ctgaaagtgc atcatggata actccttctg tttgattgca taacgaaaac
     9301 gcctcgagtg aagcgttatt ggtatgcata taaaaaagcc ctcatactgg agggcaaaga
     9361 agatttcaaa taatcagaac aagtcggctc ctgtttagtt acgagcgaca ttgctccgtg
     9421 tattcactcg ttggaatgaa tacacagtgc ttactcgtac taataaaata cccaattttc
     9481 tgtttcttgg ttgtgcccaa agttatattc aatatctggt gttgatgtat caatattctt
     9541 catcccatca acaagagttg atacaacagc caaatcctgt ttgattctca ttaaatggta
     9601 tttcttccgg cgcaataaac tttcaatggc aagtttcttc gttgggaatg caaaagatct
     9661 ttctgcattt tttgctactt tcttaattgc atatctattt ctcttttgtt tccattcctg
     9721 taaccactga tttggtgctg gtttaaaatt aacaatccaa tgcgcaggaa ccaaccatgc
     9781 ataatgctct gtctgatgaa aagctatata ttgaagtgcg aatattttga ttccatcttc
     9841 ttcaactgtc gcctggaatc tccagaaaac aggcattcca tcatgttcag tttctgattc
     9901 aggaaaaggt acgctccatg attttgtcat atctcacctc aaataagtgg cttgctgcct
     9961 aatttcattt tctggcgacc aacacaagtc acacccattt cactgcgtgg cttgctgtac
    10021 catgtgcgct gattcttgcg ctcaatacgt tgcaggttgc tttcaatctg ttcgtggtat
    10081 tcagccagca ccgtaaggtc tatcggattc agtgcgcttt ctactcgtga tttcggtttg
    10141 cgattcagcg agagaatagg gcggttaact ggttttgcgc ttaccccaac caacagggga
    10201 tttgctgctt tccattgagc ctgtttctct gcgcgacgtt cgcggcggcg tgtttgtgca
    10261 tccatcttga ttctcctgtc agttagcttt ggtggtgtgg taggtgggag acccatttcg
    10321 acctgcttcg gccgacttca attcggcaat agttccgcag gcctcgccgc tttacgtgcg
    10381 acatattccc atccatgaac ccttcaccac accccaaagc cttctgcttt gaatgctgcc
    10441 cttcttcagg gcttaatttt taagagcctc accttcatgg tggtcagtgc gtcctgctga
    10501 tggcttaaaa ttacaagaaa gattgtatgt tgtaaacaag aaatattgta aaaaggg
//
LOCUS       DAZPEU010000132        10465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPEU010000132 DAZPEU010000000
VERSION     DAZPEU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.133"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="ACHU2L_002968"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="ACHU2L_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR1357735.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="ACHU2L_002969"
     CDS             214..402
                     /locus_tag="ACHU2L_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1357736.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="ACHU2L_002970"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="ACHU2L_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR1357737.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="ACHU2L_002971"
     CDS             597..770
                     /locus_tag="ACHU2L_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR1357738.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="ACHU2L_002972"
     CDS             complement(819..1892)
                     /locus_tag="ACHU2L_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR1357739.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACHU2L_002973"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACHU2L_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="ACHU2L_002974"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="ACHU2L_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR1357740.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACHU2L_002975"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACHU2L_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR1357741.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHELSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="ACHU2L_002976"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="ACHU2L_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR1357742.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="ACHU2L_002977"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="ACHU2L_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10465
                     /gene="torD"
                     /locus_tag="ACHU2L_002978"
     CDS             10277..>10465
                     /gene="torD"
                     /locus_tag="ACHU2L_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1357743.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2524 a         2686 c         2789 g         2466 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagttcatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccacg
//
LOCUS       DAZPEU010000133        10437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPEU010000133 DAZPEU010000000
VERSION     DAZPEU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.134"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACHU2L_002979"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACHU2L_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1357744.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHU2L_002980"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHU2L_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR1357745.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHU2L_002981"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHU2L_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR1357746.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHU2L_002982"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHU2L_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR1357747.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHU2L_002983"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHU2L_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR1357748.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHU2L_002984"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHU2L_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR1357749.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACHU2L_002985"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACHU2L_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR1357750.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACHU2L_002986"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACHU2L_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACHU2L_002987"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACHU2L_002987"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR1357751.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHU2L_002988"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHU2L_002988"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1357752.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            9815..10285
                     /gene="argR"
                     /locus_tag="ACHU2L_002989"
     CDS             9815..10285
                     /gene="argR"
                     /locus_tag="ACHU2L_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR1357753.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2557 a         2657 c         2613 g         2610 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttacaaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa acagaatttg
     9661 catgccgtga cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat
     9721 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
     9781 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
     9841 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
     9901 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
     9961 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    10021 gccagctgaa ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat
    10081 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    10141 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    10201 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt
    10261 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    10321 ttgcttatcc ctctcattcc ccttctttct cctgcgatac atttaacgaa tagtgcgttt
    10381 taccgcgaca catcattcac aaaatgaata catacggtaa aaaaagcaca ttatgca
//
LOCUS       DAZPEU010000134        10169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPEU010000134 DAZPEU010000000
VERSION     DAZPEU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.135"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..2098
                     /locus_tag="ACHU2L_002990"
     CDS             <1..2098
                     /locus_tag="ACHU2L_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR1357754.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACHU2L_002991"
     CDS             2095..3183
                     /locus_tag="ACHU2L_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR1357755.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHU2L_002992"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHU2L_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR1357756.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHU2L_002993"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHU2L_002993"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR1357757.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHU2L_002994"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHU2L_002994"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR1357758.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACHU2L_002995"
     CDS             8051..8602
                     /locus_tag="ACHU2L_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR1357759.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHU2L_002996"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHU2L_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR1357760.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2650 a         2514 c         2666 g         2339 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacta tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcg
//
LOCUS       DAZPEU010000135        10082 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPEU010000135 DAZPEU010000000
VERSION     DAZPEU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.136"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            147..1276
                     /gene="ompN"
                     /locus_tag="ACHU2L_002997"
                     /pseudo
     CDS             147..1276
                     /gene="ompN"
                     /locus_tag="ACHU2L_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            1417..1851
                     /gene="uspF"
                     /locus_tag="ACHU2L_002998"
     CDS             1417..1851
                     /gene="uspF"
                     /locus_tag="ACHU2L_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR1357761.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            2027..2962
                     /gene="ttcA"
                     /locus_tag="ACHU2L_002999"
     CDS             2027..2962
                     /gene="ttcA"
                     /locus_tag="ACHU2L_002999"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR1357762.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3091..4458)
                     /gene="dbpA"
                     /locus_tag="ACHU2L_003000"
     CDS             complement(3091..4458)
                     /gene="dbpA"
                     /locus_tag="ACHU2L_003000"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR1357763.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(4488..4661)
                     /gene="ynaL"
                     /locus_tag="ACHU2L_003001"
     CDS             complement(4488..4661)
                     /gene="ynaL"
                     /locus_tag="ACHU2L_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR1357764.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4936..5919)
                     /gene="zntB"
                     /locus_tag="ACHU2L_003002"
     CDS             complement(4936..5919)
                     /gene="zntB"
                     /locus_tag="ACHU2L_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR1357765.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5933..5980)
                     /gene="ydaN"
                     /locus_tag="ACHU2L_003003"
     CDS             complement(5933..5980)
                     /gene="ydaN"
                     /locus_tag="ACHU2L_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR1357766.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6174..7406
                     /gene="dgcM"
                     /locus_tag="ACHU2L_003004"
                     /pseudo
     CDS             6174..7406
                     /gene="dgcM"
                     /locus_tag="ACHU2L_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(7427..7990)
                     /gene="smrA"
                     /locus_tag="ACHU2L_003005"
                     /pseudo
     CDS             complement(7427..7990)
                     /gene="smrA"
                     /locus_tag="ACHU2L_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(8244..9698)
                     /locus_tag="ACHU2L_003006"
     CDS             complement(8244..9698)
                     /locus_tag="ACHU2L_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR1357767.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(9695..>10082)
                     /locus_tag="ACHU2L_003007"
     CDS             complement(9695..>10082)
                     /locus_tag="ACHU2L_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1357768.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         2784 a         2505 c         2284 g         2509 t
ORIGIN      
        1 tatcatttca gaggaattat ttgattaagg ttttacttaa ggcgtaacaa atgatattta
       61 tcggctaact gaacttctcc tttaggatgt tttcactccc cgaaatgggg ataaaaggca
      121 aataaaataa ctaaaggatt tattcaatga aaagaaaagt actggcactt ttaattcctg
      181 ccctgctcgc cgcaggtgct gcacatgcag ccgaagttta taataaagac ggcaacaaat
      241 tagatctgta tggcaaagtt gatggcctgc attatttttc tgataattca gcgaaagatg
      301 gcgaccagag ctatgcgcgt ctgggtttta aaggcgaaac ccaaattaac gatcaactta
      361 ctggctacgg tcaatgggaa tacaatattc aggcaaacaa cactgaatct tcaaaaaacc
      421 agtcatggac ccgtctggca tttgccggac tgaaatttgc agattacggt tctttcgatt
      481 acggacgtaa ttatggcgta atgtacgaca tcgaaggctg gaccgatatg ctgcctgaat
      541 ttggcggtga ctcttatacc aatgcagaca actttatgac tggtcgagcc aatggcgtcg
      601 cgacttatcg taatactgat ttcttcggtc tggtaaatgg tctgaacttc gcggtgcagt
      661 atcaaggtaa caacgaagga gccagtaatg gtcaggaagg caccaataac gaacgcgatg
      721 ttcgtcatga aaacggtgac ggctggggtc tttccacaac atatgattta ggcatgggct
      781 ttagcgctgg tgcggcatac acctcttctg accgcaccaa tgaccaggtt aaccatactg
      841 cggcgggtgg tgataaagca gacgcgtgga ctgctgggct aaaatacgat gctaacaata
      901 tttacctagc aaccatgtat tcagaaacgc gtaatatgac cccgtttggc gacagcgatt
      961 atgcagttgc gaacaaaacc cagaactttg aagttactag taccagtttg atttttgtct
     1021 gcgtccggca gtctctttcc tgatgtctaa aggccgtgac cttcacgctg ctggtggtgc
     1081 agacaacccg gcaggtgttg atgataaaga tctggttaaa tacgccgatg ttggcgcgac
     1141 ttactatttc aataaaaaca tgtccaccta tgttgactat aaaatcaacc tgttggatga
     1201 agatgacagt ttctacgctg ccaacggcat ctctaccgat gatattgtcg ctttaggtct
     1261 ggtttatcag ttctaaatcc tcctgcccgc tgttatggcg ggctttttct gcttattctt
     1321 cctcctttga tctaaattaa aaatgtgaac tccgtcatta cacaaaaagt gtcatctggc
     1381 gttacacttt atgcggatac taaaacagga ggttttatga acagaacgat tcttgtccct
     1441 atcgatattt cagattcaga attaactcaa cgcgtgatta gccacgttga ggcagaggca
     1501 aagattgatg atgcagaggt tcatttcctg acggtaatac cttcactgcc ctactatgcc
     1561 tctctgggtt tagcgtattc cgcagaatta ccggcaatgg atgacctgaa agcggaagcc
     1621 aaatcgcaac tggaagagat cattaaaaaa tttaaactgc caaccgacag agtgcatgtc
     1681 catgttgagg aaggctcgcc caaagaccgc attctggaat tggcgaagaa gatccccgct
     1741 catatgatca tcattgcttc ccatcgaccg gatatcacca cttatctgct cggttccaac
     1801 gccgcagctg tagtgcgtca cgcagagtgc tccgtgctgg ttgtgcgctg acactaacgc
     1861 ccgcacattg ctgcgggctt tttgattcat ttcgcaaatg tgctgacatt ttcccctcta
     1921 atccgtacca tacacgccac agtttttata tcagatttct tatgctgagc gttccggcat
     1981 gacgcatact cttctgatgc catataacga attgagtcgc ttttaaatgt cgcaaaatca
     2041 agaaattagt aagaaagaac aatacaacct gaacaaatta caaaaacgtc tgcgtcgtaa
     2101 cgtgggcgaa gccattgctg acttcaatat gattgaagaa ggcgatcgca tcatggtttg
     2161 cctctccggg ggtaaagaca gctataccat gctggagatt ctgcgcaatt tgcagcaaag
     2221 cgcgccaatc aatttttcgc tggtggctgt taacctcgat caaaagcaac cgggcttccc
     2281 ggaacacgtt ctgcccgagt atcttgaaaa gctgggcgtt gagtacaaga ttgttgaaga
     2341 gaacacttac ggtatcgtga aagagaagat tccagagggc aaaaccactt gctcactgtg
     2401 ttctcggcta cgtcgcggta tcctttatcg caccgcaacg gaactgggga cgacgaagat
     2461 cgcgttgggt caccatcgtg acgatatcct gcaaacgttg ttcttaaata tgttctacgg
     2521 cggcaagatg aaaggtatgc ctccgaaact gatgagcgat gatggcaaac atatcgttat
     2581 tcgtccactg gcctactgcc gcgagaaaga tattcagcga tttgccgatg caaaagcgtt
     2641 cccgattatt ccgtgcaacc tgtgcggttc acagcctaac ctgcaacgtc aggtgattgc
     2701 tgacatgttg cgtgactggg ataaacgtta tccagggcgt atcgagacga tgttcagcgc
     2761 gatgcagaat gtggtgccgt cgcatctgtg cgataccaac ctgttcgatt tcaaaggcat
     2821 tacccacggt tctgaagtgg ttaacggggg tgatctggcg tttgatcgcg aagagatccc
     2881 actacaaccg gcgggctggc agccagaaga agatgaaaat cagttggatg agttacggct
     2941 gaatgtggtt gaagtgaaat aaccaggata gcgcccgatg cgcaagcgta tcgggctact
     3001 cttatggagg ccggataaga cgcggccagc gtcgcatctg gcaatcccga ataagatgtt
     3061 tactcttgca cccggcaatt caacatttca ttattttaat aaccgcaccc ggcacgtttt
     3121 tcctttaatc ttcccgccct gtaactgttt ccatgcttta tgagcaacag cctgacggac
     3181 cgcgacatag acatgcgccg gatgcacggc gattttgcca atatctgcgc catcaagccc
     3241 gatatctcct gtcagtgcac ctaatacatc acccgggcgc attttggctt ttttcccgcc
     3301 atcgatacac aacgttgcca tttctgcttc cagcgtcaca atggaactat tagctggcgg
     3361 cgtttgccag ttaagtttta tctgcaacat gtcagaaatg atattggccc gctgtgcttc
     3421 ttccggagca cagaaactga tcgccagacc gctatttcct gcacgagctg tacgaccgat
     3481 gcgatgtaca tgaacttcag ggtcccacgc cagctcaaag ttcaccacca gctcaagcga
     3541 tttaatatcc agaccacgcg cagcaacatc agtcgcaacc agtacacggg cgctaccgtt
     3601 agcaaaacgt accagggtct gatcgcgttg ctccagatcg ccgtgtaacg acaatgcact
     3661 ttgctctact tcattcagcg cgtcgcagac agcctggcaa tcttttttgg tattgcaaaa
     3721 caccacacaa gaggatggct gatgcaagct taataaccgt tgcaacagag gaattttgcc
     3781 tttactggat gtctcataaa attgttgttc aatgggtggc aaagcatctg ttgagtcaat
     3841 ttcaatcgcc aaaggatcgc gttgcactcg tccgctgatt gcggcgatgg cttccggcca
     3901 ggttgccgaa aacagaagcg tctgtcgaga tgcaggcgca aaacggatga catcatcaat
     3961 ggcatcgcta aatcccatat ccagcatgcg gtcggcctca tccatcacca aagtattcag
     4021 cgcatccagt gataccgtgc ctttttgcaa gtgatccagc aaacgacccg gcgttgccac
     4081 gataatatgc ggcgcatgtt gcagcgaatc acgctgcata ccgaacggtt gaccaccgca
     4141 caacgtcaaa attttggtat ttggcagaaa acgcgccagc cgacgcaatt cacctgccac
     4201 ctgatccgcc agttcacgcg taggacacag cactaaagcc tgggtttgaa atagcgacgc
     4261 atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt ttgccgctgc cggttttcgc
     4321 ctgcacgcga acatcttttc cggcaaggat cgccggaagc gcggcggcct gcaccggcgt
     4381 catggttaaa taacccaact cattaaggtt cgtgagttgg gcgggaggca aaacattcag
     4441 ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga cgcaaagtta gcaggcgcgt
     4501 atcctcgcag atctacgctc acgatgcgac aatttaatcg gttcttcatc gggtggtggg
     4561 tcaggcatgg gttgcgggcg agggatcgga tcgggcactg gaacaggatc gccaggaatc
     4621 ggttcaggga caggaatttg caaataaata agtgtcgtca tatttccctc cggtcattgg
     4681 gtggactctt aaagggtaga cgctgataaa taacaggcaa aaaaaagccg actcatcaaa
     4741 gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca aaaaaggaag taagacaata
     4801 tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc aagggagata ttgccctgaa
     4861 tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg tttttgtata cagacagccg
     4921 gaaaaattgc ttttgttaca accatttact acggtgcaac cataaagcaa caccaccaat
     4981 aagaacaact aacagaatac aaaaaattga aaatccgaat tgccacccgc cgccagggat
     5041 cccaccaagg ttgacgccaa ataacccggt cagaaaggta ctgggtaaaa agaccattgc
     5101 catcaacgac attgtatagg tacgacgagc taaattttcc tgcatcacct gagcgatttc
     5161 atccgccatc acgccagtcc gtgctataca ggcgtcgatt tcgtcaaggc cgcgcccaag
     5221 gcgatcggca atatcctgca tccgacggcg ttggtcatcg ctcatccacg gcaaacgttc
     5281 actggcaaga cgagcataaa catcacgttg cggtgccata tagcgacgca tcacaattaa
     5341 ttgtttgcgc agcagagcca ggaatccacg cggtggaatt tgctgatcaa ggagattatc
     5401 ttcaaggtcg ataattttat cgtgcagctg ctcgataaat tcactggaat gatcggtcaa
     5461 cgcatcgcac acatccacca gccatccccc gcaatcggtc ggacccgtgc cctcttccag
     5521 atcgctcacc acatcgtcca gcgccagcac tttgcgttgt cgggtcgaaa caattaaccg
     5581 cccgtccata tatacacgca tggcgaccag ttgatcgggg cgttcatcgg tgctgccgtt
     5641 tatacagcgc aatgtaatca gcgtgccttc accgaaacgg ctgactcggg gccgtgtgct
     5701 ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc ggtgttgtcg ccagccattg
     5761 ggcgctatca tggtgtacat aattaaggtg gagccagcag ggatgcgctt catcaatcac
     5821 atctgtattt tccagcggtt taacgccgcc tctaccatcc agcatccagg caaatactgc
     5881 atccgggaca ttaacgtccg atcccttaat cgcttccact gtgcctccat catcaacgca
     5941 ttattttgta gtctagcctt ctggccctgt tacgcaacat ctcatcaccc cattaccctg
     6001 aaatgattaa taaaattctg tctaaattga atacaaaaag caaaatgctt ttccgtatac
     6061 aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga attatctgat catatgacgt
     6121 ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa taacgggatc tcaatgatta
     6181 cgcacaactt caataccctg gacttactca ccagtcctgt ctggattgtt tcgccctttg
     6241 aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt gatgcaagac ctcacgttta
     6301 gtcagctacg aaccggaccc tattccgtct cctcacaaaa agaactgccg aaatacctct
     6361 ccgatctgca aaaccaacac gatattatcg aaatcctcac tgttcagcgt aaagaagagg
     6421 aaacagcatt aagctgtcgg cttgttttgc gagagctgac agaaacagaa ccggtgatta
     6481 ttttcgaagg tatcgaagcg ccggcaacgc tgggtttaaa agccagtcgc tcggcaaatt
     6541 atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct gactaactct gcaccaatgt
     6601 tgttgattga cccgtcacga gatggacaaa tcgtcgatgc taacctcgcc gcgctcaatt
     6661 tctatggtta taaccatgaa acgatgtgcc agaaacatac ctaggaaata aatatgcttg
     6721 ggcgtcgcgt catgcctatc atgcatgaaa tttcgcattt acccggcggt cataaccctt
     6781 tgaattttgt tcataaactg gcggatggat ctactcgtca tgtgcagacc tatgccggac
     6841 cgattgaaat ttatggcgac aagctcatgt tatgtattgt gcatgatatt actgagcaaa
     6901 aacggctgga ggagcagctg gaacatgctg ctcaccatga cgcgatgacc ggattactga
     6961 atcggcgaca gttttatcac attacggaac caggccaaat gcagcatctc gccatcgctc
     7021 aggattacag cttgttgctc atcgacaccg atcgttttaa acacattaac gatctctatg
     7081 ggcattctaa aggtgatgag gtgttatgcg ccctcgcccg ccccctcgaa agttgcgctc
     7141 gcaaaggcga tttggtgttt cgttggggag gcgaagagtt tgtcttattg ctaccaagaa
     7201 ccccactgga taccgcgctt tcgctggctg aaactatccg cgtaagcgtg gcaaaagtga
     7261 gtatttcggg cttaccacgc tttaccgtca gcattggtgt ggcgcatcac gaaggaaatg
     7321 aaagcatcga tgaactgttt aaacgcgttg atgatgcttt gtatcgggcg aaaaatgatg
     7381 gacgcaaccg cgtgctggcg gcataagccg cggatgcgtc tcgagatcaa cgactgcgct
     7441 tagcgtggcg ctcccagttt tcttgcttcg cctgcgccgt tttacgtagt gcgacgtaac
     7501 acgccccgct gccgccatga tgcggtagcg cggtgcaata tgcctgaaca tcatcaaatt
     7561 cggtcagcca gcgcgccaca tagctgcgga caatattggc atgtgattta tcttcccgcc
     7621 ctttaccatg aataatcagc acattacgca aaccatccgc cagggcttgt tgaatgaaac
     7681 tgaacaccat tttgcggcac tcttccaccg gctggcgcaa aagattcagg ctcgcctgtt
     7741 gcggatattt accactgcgc agcttatcca gcaccccatg ctacaaccct tcctgccgaa
     7801 actccagcgg ctgacttagc gggatgatgt cgagaaatcc ggtagtgagg aaattatcaa
     7861 gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg cgttggatgc cagtggacat
     7921 ctgtagcacg tttcagcggc tggacatctt ccatggcgtc aagaaacagc gatttgtcgt
     7981 caaggttcat gtaacctcct atgcaccttc acacgtcgtg attcagacgg gttaattgtt
     8041 catacttact gcatcgttcc cttcacagat tgaaacaata ctctacttca tacctgtgag
     8101 tatctgcgca ggcggggcat tttatactag cctgttagca gtatcgaaca gtttagcccg
     8161 agtttaattc agcacaggct ccgatcagga tgatttattc atagcaggaa ccaacaaaaa
     8221 tgcgataaat aacagaccac tgattagcag aaaggtaccg aaaatgccat agtttgcgcc
     8281 tccctcagca tcaattgact caataatttg gttgaagcca gtatttccga tgtctggtca
     8341 cttctactgc gattaaaaaa taaagcactt ctggaaatga agtcattcac cgtatgggga
     8401 acagaaacca ttttttcacc cattctcgaa aaatgaaaat tagttcgatt attgagaata
     8461 gagccacaca gggcaatacc aatcgcgccc ccaagattac gtgttaaatt aaatacacca
     8521 gaggctaact tcagccgttc ttttggaatt ccccccagcg ttaatgttac gatgggggcc
     8581 atcgcaaact gctgcgaaat ccctctaatt gcctgtggaa acagtaattc ctgccatccc
     8641 cattcgtggg ttataggggt aaaaagatac attgaaaaca caaatccgcc cagccccgcc
     8701 atcaacagcc atcgcagatt tattttttta cttaaccaga agtaaaaggg aacagaaaat
     8761 aactggaaaa tacctgttgt gcaaacggca aaacctatct cttcggcatt taatccgcga
     8821 atctgcccca aaaacacagg tattaaatat acagtagaga atatcccaac ccctccagaa
     8881 aaagaaaaaa aacatcccag agtgaaattt ttatccttaa aagcatgaag atccattatc
     8941 ggattggata ttgtaagtgt acgcgcagca aataaaataa aactcaccaa tgccagaact
     9001 gatgtcagca aaatagtatt atcatccagc catccccagc gcgctccttc ttcaagggta
     9061 tattccagac atcccagagt catggccaac aggataatac tcggataatc agcaactttc
     9121 agtaaagaga gatcaggttt atcaaaatta accagaaaag gaatactcaa aactaaatat
     9181 atgccaggta atatattgat ataaaatagc catcgccagt cgaggttatc agtgagccag
     9241 ccccccagag ttggccctag cgtcggtgac agtgaggcca gcgcactgac taccgcagca
     9301 gccaacccca actctttacc ctggtaataa ataaacgcca tcgtgaaaac taaagggatc
     9361 atggatgcgc cagcagctcc ctgtaaagca cgaaacaaaa tcatgatctg aatattccat
     9421 gcaagtccac acgcgatact cattaaagta aatattcccg cggatagagt aaaaagccaa
     9481 cgagtggaaa atacccgtga cagccaccca gagagcggaa taacaataat ctcagcaatt
     9541 aaatagctgg tctgaagcca ggccatttcg tctttacctg cggacagacc accacctatt
     9601 tcatttaggg atgacgatac aatttgaata tcaagctgag caataaataa tcccatacac
     9661 atactggcga aagcaatatg tttacgcata gacatcatct ctcatcaaca ctgacggtga
     9721 cggacagtcc cgggcgaaga agttgcttta attcctttgc gtcgtcaaat gcaatccgta
     9781 ccgggacacg ctgaacaatt ttggtgaaat tgccagtggc attttctatc ggaatcaagc
     9841 tgaaactggc tcctgtcgct ggggaaaggc tttcaatgtg accgtgaaac acttcccctt
     9901 tgagaatatc agcacggatc tcagccttca ttcctggctt cattcccgct atttggtttt
     9961 ctttataatt tgcatcaacc cacaactcac tgactggtac tagtgaaact aacgatgtgc
    10021 cgccttccac ccaactgcct gtatgggcgc tgcggtttgc gacaatgcca tcaatcggcg
    10081 ca
//
LOCUS       DAZPEU010000136        10079 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPEU010000136 DAZPEU010000000
VERSION     DAZPEU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.137"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            318..1184
                     /gene="folD"
                     /locus_tag="ACHU2L_003008"
     CDS             318..1184
                     /gene="folD"
                     /locus_tag="ACHU2L_003008"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR1357769.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1186..1398
                     /gene="ybcJ"
                     /locus_tag="ACHU2L_003009"
     CDS             1186..1398
                     /gene="ybcJ"
                     /locus_tag="ACHU2L_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR1357770.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1506..2015
                     /locus_tag="ACHU2L_003010"
     CDS             1506..2015
                     /locus_tag="ACHU2L_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1357771.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(2051..3436)
                     /gene="cysS"
                     /locus_tag="ACHU2L_003011"
     CDS             complement(2051..3436)
                     /gene="cysS"
                     /locus_tag="ACHU2L_003011"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR1357772.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3610..4104
                     /gene="ppiB"
                     /locus_tag="ACHU2L_003012"
     CDS             3610..4104
                     /gene="ppiB"
                     /locus_tag="ACHU2L_003012"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR1357773.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            4107..4829
                     /gene="lpxH"
                     /locus_tag="ACHU2L_003013"
     CDS             4107..4829
                     /gene="lpxH"
                     /locus_tag="ACHU2L_003013"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR1357774.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4947..5456
                     /gene="purE"
                     /locus_tag="ACHU2L_003014"
     CDS             4947..5456
                     /gene="purE"
                     /locus_tag="ACHU2L_003014"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR1357775.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            5453..6520
                     /gene="purK"
                     /locus_tag="ACHU2L_003015"
     CDS             5453..6520
                     /gene="purK"
                     /locus_tag="ACHU2L_003015"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR1357776.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6657..7550)
                     /locus_tag="ACHU2L_003016"
     CDS             complement(6657..7550)
                     /locus_tag="ACHU2L_003016"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1357777.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7547..8194)
                     /locus_tag="ACHU2L_003017"
                     /pseudo
     CDS             complement(7547..8194)
                     /locus_tag="ACHU2L_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            8197..9429
                     /gene="allC"
                     /locus_tag="ACHU2L_003018"
     CDS             8197..9429
                     /gene="allC"
                     /locus_tag="ACHU2L_003018"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFR1357778.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            9440..>10079
                     /gene="allE"
                     /locus_tag="ACHU2L_003019"
     CDS             9440..>10079
                     /gene="allE"
                     /locus_tag="ACHU2L_003019"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR1357779.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2333 a         2711 c         2675 g         2360 t
ORIGIN      
        1 tccgcgcaaa atatattcac cacaaatata gacctgaagc ggtaaattac caggctgaaa
       61 attcttttta tattgtcagg tatttcttaa attatcttaa tccttaaaca aggaaataaa
      121 tcagttccag atttacaacg ccatcatgta cgaaaaatga agctttcaat ctcagcgacc
      181 tcagcgacgg tgcgcctaac cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa
      241 acctgacagc gcgccccgct tctgacaaaa tagacgcatc cccttcgacc tacgtaacag
      301 atggaatcct ctctctgatg gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg
      361 tgcgctctga agttgctcaa aaagttcagg ctcgtattgc agccggactg cgggcaccag
      421 gactggccgt tgtgctggtg ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac
      481 gcaaggcttg tgaagaagtc gggttcgtct cccgctctta tgacctcccg gaaaccacca
      541 gcgaagcgga gctgctggag cttatcgatg cgctgaatgc cgacaacacc atcgatggca
      601 ttctggttca actgccgtta ccggcgggta ttgataacgt caaagtgctg gaacgtattc
      661 atccggacaa agacgtggac ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg
      721 cgccgcgtct gcgtccctgc accccgcgcg gtatcgtcac gctgcttgag cgttacaaca
      781 ttgatacctt cggcctcaac gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa
      841 tgagcatgga actgttgctc gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa
      901 atctgcgtca tcacgtagaa aatgccgatc tgttgatcgt tgccgttggc aagccaggct
      961 ttattcccgg tgactggatc aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc
     1021 tggaaaatgg caaagttgtg ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat
     1081 acattacgcc tgttcccggc ggcgttggcc cgatgacggt tgccacgctg attgaaaaca
     1141 cgctacaggc gtgcgttgaa tatcatgatc cacagggtga gtaacatggc gacattttct
     1201 ttaggtaaac atccgcacgt tgagctgtgc gacttgctga aactggaagg ctggagcgaa
     1261 agcggcgcgc aggcgaaaat cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt
     1321 gaaacgcgca aacgctgcaa aatcgtcgcc ggtcagacag tgagttttgc aggacacagc
     1381 gtacaggttg ttgcctgatc cctttcccgg cctgtatcaa caggccggtt ctcttttcag
     1441 acttttgccc tctacgcccc gttcatccat gctgatagca tcgacatttt gccgaagcga
     1501 ccatcatgcc aaccgttatt acgcacgccg ccgttcccct ttgcattggt ttagggttgg
     1561 gttcgaaagg tctgttattt gccggaatca tcctggcgat gctgcccgat gccgacgtat
     1621 tgtcgtttaa atttggcgtt gcttacggca atgtttttgg tcatcgcggt tttacccatt
     1681 cgctggtgct tgcgtttgtt gtcccgctct tatgtgtgct gattggacgt cgatggttca
     1741 gggctgggct gattcgctgc tggctgtttt taaccgtctc gttgctatcg cacagcttac
     1801 tggattcggt aaccactggc ggtaagggcg ttggctggct gtggccgtgg tcagatgaac
     1861 gctttttcgc tccctggcag gtgattaaag tcgcgccgtt tgcattgtct cgttacacca
     1921 cagcgtacgg gcatcaggtg attatttccg aattgatgtg ggtgtggttg ccggggatgc
     1981 tactgatggg aatgttgtgg tggcgcagac gataaccgga tgcgaaaact cgcatccggc
     2041 aattgcgcaa ttacttacga cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc
     2101 ccatctcgtt aagacggtca cgtgccgcat ctgccgccgc ccagtctttc gctttacggg
     2161 catccagacg ctgttgaatt aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg
     2221 cgccgctttg caggaacgct tccggttctt gctccagcag gcccaataca gcggaaagtt
     2281 tacgcaggtg agatgccatt gcattcgccg ctgccatatc ttctgctttc agacggttca
     2341 cttcacgcgc catatcaaac agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca
     2401 tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc aggcgcaacg gttttatctg
     2461 tgccgcgcag cgcagtgtag aggcgctcca gcgccgcacg cgcctgcttc aggttctctt
     2521 cgctgtagtt cagctggctg cgatagtggc ccgacatcag gaagtaacgc acggtttccg
     2581 cgtcgtagta tttcagcaca tcgcgcacgg taaagaagtt acccagcgat ttggacatct
     2641 tctcgcggtc aaccatcacc attccggagt gcatccagta gttcacatac tgaccatcat
     2701 gggcacaggt ggactgcgcg atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc
     2761 cgccgtggat atcaaagtgg ttacccagct gcttgcagtt catggccgaa cattcaatgt
     2821 gccagccagg acgacccgcg ccccacggag acggccagct cggttcgccc tctttcgaca
     2881 tcttccacag aacgaagtcc atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc
     2941 cagcctgcag ctggtcgaga tcctgacgcg acagcacgcc ataagttgga tcggtcggga
     3001 cgtcgaacat cacgtcgctg ttgtccgcca cataagcgtg acctttggcg atcagttgtt
     3061 cagtgagttc aataatttct gcgatatggt gcgtcgcgcg cggctccata tccgggcgca
     3121 gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat catgcgatcc accagcgcca
     3181 caaagctttc gccattttca ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt
     3241 tgcgcacata cttcagttta tagccgagga aacgcagata gcgcgcaacc acgtcaaaag
     3301 caacaaaggt acgcccgtga ccgatatgac agagatcgta aacggtgatt ccacacacgt
     3361 acatgccgac ttccccggcg tgaataggct taaattcctc tttttggcgt gtcagagtat
     3421 tgaagatttt tagcatcgaa gattccgttt agacatgtgt gggtaattga gttgcgtata
     3481 atacccatat ttcccgcgcg aatcagcata cattgcgtga tgatccgatc gtgcggttat
     3541 gctataacac caccctatat atgacccgaa ctgggttgaa gcaccaatca aacggaacag
     3601 gatgcaaaaa tggttacttt ccacaccaat cacggcgata ttgtcatcaa aacttttgac
     3661 gataaagcac ctgaaacagt taaaaacttc ctggactact gccgcgaagg tttttacaac
     3721 aacaccattt tccaccgtgt tatcaacggc tttatgattc agggcggcgg ttttgaaccg
     3781 ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa
     3841 aatacccgtg gtacgctggc aatggcacgt actcaggctc cgcactctgc aactgcacag
     3901 ttcttcatca acgtggttga taacgacttc ctgaacttct ctggcgaaag cctgcaaggt
     3961 tggggctact gcgtgtttgc tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa
     4021 ggtgttgcaa ccggtcgtaa cggtatgcac caggacgtgc caaaagaaga cgttatcatt
     4081 gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt tattgcagat cttcatctct
     4141 gcgtggaaga accggcgatc accgccggtt ttctgcgttt tttagcgggg gaagcccgca
     4201 aggccgacgc gctgtatatt cttggcgatc tgtttgaagc atggattggc gacgacgatc
     4261 ccaacccact ccatcgccag atggcggcag cgatcaaagc ggtgtccgat tccggcgttc
     4321 cctgttattt cattcatggc aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa
     4381 gcggcatgac attattgccg gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga
     4441 ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag
     4501 tccacaaacc ctggctgcaa atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca
     4561 ttgccgcgcg aatgcgcgcg aacagcaaag aagccaacag cagtaaatcg ctggcgatca
     4621 tggacgttaa ccaaaacgct gtggtcagtg cgatggaaaa acatcaggtg caatggctaa
     4681 tccacgggca tacccatcgc ccggcggtgc atgaacttat cgccaaccag caacctgctt
     4741 ttcgcgtggt actgggtgcc tggcatacgg aaggttcgat ggtgaaagtc acggctgatg
     4801 acgttgagct gattcatttt ccgttttaaa aaacccacaa ctttgctgat ttcacagcca
     4861 cgcaaccgtt ttccttgctc tctttccgtg ctattctctg tgccctctaa agccgagagt
     4921 tgtgcaccac aggagtttta agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca
     4981 tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa
     5041 tcctgaatgt cccgcaccac gttgaagtgg tttctgctca ccgcaccccc gataaactgt
     5101 tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt gattattgcg ggcgcaggcg
     5161 gcgcagcgca cctgccaggc atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc
     5221 cagtacagag cgccgcactg agcggtgtcg atagcctcta ctccatcgta caaatgccgc
     5281 gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg cgcggcaaac gcggcattac
     5341 tggcagcaca aattctcgcg actcacgata aagagctaca ccagcgtctg aatggctggc
     5401 gcaaagccca gaccgacgaa gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca
     5461 ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt
     5521 aggcattgct gtctggccgg tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca
     5581 aagcgtgatt accgctgaga tcgaacgctg gccggaaacc gcattaaccc gcgagctggc
     5641 gcgtcatccg gcctttgtga accgcgatgt gttcccgatt attgctgacc gtctgactca
     5701 gaaacagctt ttcgataagc tccacctgcc gaccgcgccg tggcagttac ttgccgatcg
     5761 cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac
     5821 tggtggctat gacggtcgcg gtcaatggag tttacgcgcc aatgaaaccg aacagttacc
     5881 ggcagagtgt tacggtgaat gtattgtcga gcagggcatt aacttctctg gtgaagtatc
     5941 gctggttggc gcgcgcggct ttgatggcag caccgtgttt tatccgctga cgcataacct
     6001 gcatcaggac ggtattttgc gcaccagcgt cgcttttccg caggccaacg cacagcagca
     6061 ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat
     6121 ggcgatggag tgttttgtca ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt
     6181 gcataacagc ggtcactgga cacaaaacgg tgccagcatc agccagtttg agctgcatct
     6241 gcgggcgatt accgatctgc cgttaccgca accagtagtg aataatccgt cggtgatgat
     6301 caatctgatt ggtagcgatg tgaattatga ctggctgaaa ttgccgctgg tgcatctgca
     6361 ctggtacgac aaagaagtcc gtccggggcg taaagtgggg catctgaatt tgaccgacag
     6421 cgacacatcg cgtctgactg cgacgctgga agccttaatc ccgctgctgc cgccagagta
     6481 tgccagcggc gtgatttggg cgcagagtaa gttcagttaa gcaggtgctc atttgattgc
     6541 cggatgcggc gtaaacgcct tatccggctt accgctccgg tacccattgt aggcctgata
     6601 agacgcgtca agcgtcgcat caggcatggt gcaccgattg ccggatgctc tcatcactac
     6661 agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt caattcgcga taacgcccca
     6721 atccacgcgg gtttactacg gcttctgaca taaccactca ccgccgttac cttcggcccc
     6781 atcgaaccat cggctttggc aaatggcgct aactcatccg gtgtggcatg gcgaatggca
     6841 cgttgctgcg gcgttcccca gttttcatat accgcatcag catcggtgag gatcaccagt
     6901 ccatcggcat taatctgctc ggcgagcaac gcagcggcga gatctttatc aatcaccgct
     6961 tcactccctg ccccatcttc cgtcacaggc acaccgccgc cgccactgca aatcaccaca
     7021 tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga gaattttacg cggttgcgga
     7081 gacgccacca cccggcgcaa atatttacca tcacgtttca tctgccagcc gtaagccgct
     7141 tccagtgcct cttgttcttc tggctgataa accggaccaa taaatttctc tggctgcaaa
     7201 aacgccggat catcaggcga aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc
     7261 tgcggctgtg cgctcaagct ctgcgccagc atatagccaa tcatcccctg gctttccgcc
     7321 accagtacat ccagcggata cggttctacc tctttccacg ccagattctg taacgccagc
     7381 agccccacct gcggcccgtt gccgtgaaca atcgccagcc gataagaacg ggccaggcgt
     7441 gccagcgcgg gtacagcact ggcgatattg cgatattgat tttccgccgt cagcgcctca
     7501 ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca gtgttttcat ggttttcctt
     7561 gtaataattg ttggccaagc cagaacccca gcagcgtatc tgcccctgac gtatgcccca
     7621 gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa
     7681 agtgcaggag tggcgaggcg aaatatcctt gcgcggcata acgtaaataa ctgacgctca
     7741 ccgctgtggt aacgagttga agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg
     7801 catctaaagc gccataatac caggccgcca gcagcattcc gctcaacgtg tcatcatgac
     7861 ttggcgttaa tccggggcct ttacccagcc agtgccgcca gtcggtctta acgccattga
     7921 gcgcggcctg aaaacagtga cgaaactggc gtaattcagc gggcagcgga tcgcttgccg
     7981 ccatcgccag tggcccgaaa agtccggttt cctccgcgcg ttgcatccat gcagctgcaa
     8041 gcggttgagg atgcgaaggc ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa
     8101 ccgtgaaacg cccgaggcga atcccttgag ccacaacctg tggtcgttca ttgccgcata
     8161 atccaccgca taacgcatcg aattgcgtgc ggcgaaatta cacattcccg tcaagctata
     8221 gaagaaacgc tgccctggct ttcctctttt ggcgctgacc caacgggtgg gatgacccgt
     8281 ttactttatt cgccggaatg gctggaaacc cagcagcaat ttaaaaaaag aatggcagca
     8341 agtgggctgg aaacacgttt cgatgaagtg gggaatttat acggtcgcct gagtggcacc
     8401 gaatatccac aggaagtggt tctgagcggt tcgcatatcg ataccgtggt taacggcggt
     8461 aacctcgacg ggcaattcgg cgcgctggcg gcgtggctgg caattgactg gttgaaaacg
     8521 caatacggcg caccgttacg tacggtcgaa gtggtggcga tggcagaaga agaaggcagc
     8581 cgcttcccgt atgtcttctg gggcagtaaa aatatctttg ggctggcgaa tcctgacgac
     8641 gtgcggaata tctgtgatgc caaaggaaat agttttgccg atgcgatgaa ggcttgcgga
     8701 tttactctgc cgaacgcccc actaactccg cgtcaggcta taaaagcctt tgttgaactg
     8761 catattgaac agggctgtgt gctggaaagt aatgggcaat caattggcgt ggtgaatgca
     8821 attgtcgggc agcgtcgcta tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc
     8881 acgccgatgg gttatcgtcg tgatacggtt tacgctttca gtcgcatttg ccatcagtcg
     8941 atcgaaaaag cgaaaaagat gggcgatccg ctggttctga cctttggcaa agtagagccg
     9001 cgcccgaata cggtaaatgt ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat
     9061 accgacgctg ccgtgctgcg cgatttcacc caacagttag aaaacgacat gcgggcgatt
     9121 tgcgatgaaa tggacattgg tattgatatc gatttatgga tggacgaaga acccgtgccg
     9181 atgaataagg agctggtcgc caccctgaca gaattgtgtg aaagagaaaa actgaattac
     9241 cgggtgatgc acagtggtgc cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc
     9301 tgcatgattt ttatccccag catcaacggg atcagccata acccggcgga acgcaccaat
     9361 attactgacc ttgccgaagg ggtcaaaacg ttggcactca tgctttatca acttgcctgg
     9421 caggaataag gagtcataaa tgggatattt aaataacgtc accggttacc gcgaagattt
     9481 actggctaac cgtgcgattg ttaaacacgg taatttcgca ctgttaaccc cggatggtct
     9541 gataaaaaat attattccgg actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa
     9601 gctgggtgcc tcttttgttg attatctggt cacactgcat caaaacggtg gcaaccaaca
     9661 gggcttcggt ggcgaaggca ttgaaacgtt cctgtatgtg atctctggaa atatcactgc
     9721 caaagccgaa ggcaaaacat ttgccttaag cgaaggtggc tatctttatt gcccaccagg
     9781 ctccttaatg acgtttgtta acgcccaggc cgaagacagc caaatctttt tatataagcg
     9841 ccgctatgtt ccggtagaag gccatgcacc gtggctgttt tctggcaatg ccagcgaact
     9901 ggaacgcatt cactatgaag gtatggatga tgtgatcctg ctggattttc tgcccaaaga
     9961 gttaggtttt gatatgaaca tgcatatcct ctcttttgca ccaggtgcca gccacggtta
    10021 tatcgaaaca cacgttcagg aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DAZPEU010000137        10047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPEU010000137 DAZPEU010000000
VERSION     DAZPEU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.138"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..756
                     /locus_tag="ACHU2L_003020"
     CDS             <1..756
                     /locus_tag="ACHU2L_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR1357780.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHU2L_003021"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHU2L_003021"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR1357781.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="ACHU2L_003022"
     CDS             complement(1792..1995)
                     /locus_tag="ACHU2L_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1357782.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHU2L_003023"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHU2L_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR1357783.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="ACHU2L_003024"
     CDS             complement(4588..5100)
                     /locus_tag="ACHU2L_003024"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR1357784.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHU2L_003025"
                     /pseudo
     CDS             complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHU2L_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            complement(6903..7840)
                     /locus_tag="ACHU2L_003026"
                     /pseudo
     CDS             complement(6903..7840)
                     /locus_tag="ACHU2L_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHU2L_003027"
     CDS             complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHU2L_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR1357785.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8767..>10047)
                     /gene="hpaX"
                     /locus_tag="ACHU2L_003028"
     CDS             complement(8767..>10047)
                     /gene="hpaX"
                     /locus_tag="ACHU2L_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR1357786.1"
                     /translation="RRLIIFLFILFVFSFLDRINIGFAGLTMGKDLGLTSTMFGLAAT
                     LFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVWGIASTCTMFATSPETLYLLRMLVGI
                     AEAGFLPGILVYLTWWFPAYHRARANALFMIAMPVTMMLGLILSGYILALDGLWNLKG
                     WQWLFLLEGLPSVILGVVTWFYLNDTPDQATWLDENEKQALKTMILREQESAFAHASV
                     PRSTLREVLTPAIMLYTLAYFCPTNTLSAINIWTPQILQSFNTGSSNIMIGLLAAIPQ
                     FCTIFGMIWWSRRSDRLKERKKHTILPYLFAAAGWLLASATDHSLIQLSSIIMASMGS
                     FTAMTIFWTTPDQVISLQSRAVAMAVINAVGNVGSAVSPLLIGILKDATGSFSSGLWF
                     VAGLLVLGALVMTRIPMGSREHSLSSELIAPKPQ"
BASE COUNT         2573 a         2487 c         2551 g         2436 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tctgctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaaacaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt ttatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag caggttttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccacctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agagctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagcgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg gtcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agccgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgttga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat cgttctcatc gaagcggctg gagagcgggt agtcgtatgg cgagccggta
     5941 gcacccgcga ccatctcata agaggcgcgg gagattaatt tcacgccatc ggcatccatt
     6001 ggcgcaacga acatcagtgc gaagtccggg ttttcgccca tcacttgtgc cgagccgaag
     6061 ccaatcatgt tgtagtgagt cagcgccgag ttggtggcaa ccactttcgc accgctgacg
     6121 ataatcccgg cgtcagtctc tttttccagc ttgatgtaaa cgtctttcac tttatcggtc
     6181 ggcaaatgac gatcggtggg ttaacaatcg cgtggttaaa gtagaggcca gtttcctgaa
     6241 tacgggtata ccagttacgg gcgttctgct cgaactgacc gtaaaagccc ggattcgcgc
     6301 ccagtgcgca accgaaagcg gctttgtagt ctggggtacg gcccatccag ccatagctca
     6361 ggcgtgacca ttcagcgatg gcgtcgcgtt gctggcgcag gtcgtcggca cttttcgcca
     6421 cgcggaagaa tttatgggta tagccgccgc tgccggtgtc ggtgttccaa cacagagagt
     6481 cctgcatctc cggtttgtgc agcgcgtcgt acagttgggc aacagacgct gccgcattac
     6541 gaaatgccgg atgagtggtg acgtctttca ctcgctcgcc atagatatag atctcgcgac
     6601 catcctgcag gcttttcaga tactcttccc cggtgaaagg acgttgggta ctggcgcgga
     6661 aatcttctgg tttcatgtcg acctctacag tattaatcgg aatacgattg ttaattttgt
     6721 gtgttgtttt tgttatttaa ttgaagcgtt gaacgcggtt gtcgggaaga gacgtttcag
     6781 gcgaacgttg gtacttttcg acagcaaggt ggagttaaat agtaaactgc gatcgtgatc
     6841 aacttacagt aaggcaaatc ataatattcc ccgctaacaa gggctaacgg ggacggaatt
     6901 ttttatgggg cgaatggaat ggtaatcgtc atattttcat gaccaacgac gataggacct
     6961 gaatagtttt ctgtgcttct tgcttccatg tttcatccgg aacggtgatt ggtaccaggt
     7021 ggctaaggat gagttttttc acttttgctt cattggcaat tttgcctaca tcctcaatag
     7081 tcgtatggtg agaagcaatg gcttttgcca gtgttttacc attgccaata ctgtctgcca
     7141 gcttagcgac ggagggctca tgtacggcct catgcacaag tatatcagcg ccttctgaga
     7201 aagttgccag tgggggaaag tacgaggtat ctcctgagaa gacaattcgc ttgccttgtg
     7261 tgtcgaagcg ataagcaaaa gcctcaccgt cagggaaagg agaatgaggg acctttaatg
     7321 cagtcacggt cacggtatca tctttataaa ctacgccttc accaacatct tgagctgtaa
     7381 cgagtttgcg aggatcgggt tttccttctt cttccatacg cagtgtgatg tcccgatcga
     7441 agacctgcca actggcagcg gtcatggctc gggtgcctgg cggaccgaat acatgaatag
     7501 ttttgtcctg cagaccgcta gcccatgcat tcattaagac tgatggatag tccagaatat
     7561 gatcactgtg taaatgggtg agtaaaatat tatcaatctg gcgcagtgga atattatttt
     7621 ttaccagttg taagctggcc ccgtaaccag catcaattaa ccaggtttta ttgccaataa
     7681 taagtgccgt tgccatcggt aaacggtcag cttttaataa agaagggcca cctttcgttc
     7741 caagtagtac aattttcatt tattgcggtt cggaggcgta ggtgaatgcc actggcgcaa
     7801 tcaggcacgc cacaaatagt gagcgtaaag atattttcat gtcgagtctc tcaggttgat
     7861 aaatggtcag cgaccacgga attgtgttgg tgagcaaccc atcattcggt tgaaaaaacg
     7921 ggcgaaatag gcgggatctt tgtagccaag atcaagggct atctggttga tgctttgctc
     7981 gctatagagc agaagccgtc tggcttcccg gctcactcgt tcgaaaatca atctcttcgg
     8041 cgattgattg gcaaaacgtc ggcacagctc ggtaagccgt gattcactca taccgagcat
     8101 tgttgcatat tcagggacca ttaaatgttg gcgataattt tcgtcaatca gccgattaaa
     8161 cttctggaat aaacacatct cgccgcgcac gctgcatgtt gaaatatcat ttggtggtgc
     8221 ttcacgcagt aggattgtaa aaatactttg cgccagtgat tgcaatagaa tttcccgtcc
     8281 ttgcttattt tgttgaaact ccagggatat tatcggccaa taatgattta atgccgccag
     8341 tgtatgttcc attccctcca gtgacagaca gataccttgt agcattgcgg catctccact
     8401 accaggccag agcttttcta ccaggggcca aattaactcc tgacgtacgg tcagaacatg
     8461 cccatcgcta tcaggatcag taaagaaagt atgtggcacc gatggtggtg ttagtacaaa
     8521 aagcggtgcg cgcacagaat agaagtgctc atccagttgt aaagtaatgc gtccagtgac
     8581 cagataatgg agttgaaaat atctatcgtg ccagtgaggg cgcatatcgc gtccaaagaa
     8641 attcgctaac cgggcaaatg tttcataatg cacatcttca ccagcgtagc gggcatcata
     8701 ttcccggacc aaatcaatat tttcataacc atgttcaaag atagcttcac tggacatcat
     8761 gactccttat tgcggttttg gtgcgataag ttcagatgaa agagagtgtt cccgactccc
     8821 catagggatt cgggtcatga ctaacgcgcc cagtaccagc aatcctgcta caaaccacaa
     8881 acctgagcta aaactgccgg ttgcatcttt caaaataccg ataagtaatg gactgactgc
     8941 tgaacctaca ttgccgacag cattgatgac agccatcgca acggcacgcg attgcaagct
     9001 tataacttga tccggtgttg tccaaaagat agtcatcgcg gtaaatgatc ccattgaggc
     9061 cataatgatg ctgctcagtt gaatgaggct gtgatctgtc gcagaagcga gtaaccatcc
     9121 ggcagcggca aacaggtagg gaagaatcgt atgctttttg cgttctttaa gacggtcgga
     9181 acgacgactc caccagatca tgccaaaaat tgtgcagaat tgggggatgg ccgccagcaa
     9241 accaatcatg atgttgctac tgccagtatt gaaactctgt aggatttgcg gcgtccagat
     9301 attaatcgca ctcagagtat tagtcgggca aaaataagca agtgtatata gcatgatggc
     9361 tggggtcagg acttcacgta aggttgagcg tggaacagat gcatgggcaa atgcactctc
     9421 ttgttcacgg agaatcatcg tttttaaagc ctgtttttcg ttttcatcaa gccaggtcgc
     9481 ctggtctggt gtgtcgttga gataaaacca ggtgacgaca ccaagaatga ccgatggcag
     9541 accttccagc aaaaagagcc attgccagcc ttttagattc cacagtccat ccaaggccag
     9601 aatgtagcct gaaaggatca atcccagcat catagtgact ggcatcgcta tcataaataa
     9661 ggcattggct ctggcccgat gatatgccgg gaaccaccag gtcagataaa ccaaaatacc
     9721 aggtaaaaaa cccgcctcag caataccaac cagcatacgc agtagataga gtgtttcggg
     9781 gcttgttgca aacattgtgc aggtggaagc aattccccag accaccatga tcccagcgat
     9841 ccatcgacga gcgccaattt tcgctaacat catattactg gggatcccgc acatgacgta
     9901 cgtgacataa aagagtgttg cggcgaggcc aaacattgtg gacgtcaggc caagatcctt
     9961 gcccattgtc agtccggcaa atcctatatt gatgcgatca agaaacgaaa aaacaaacag
    10021 aataaaaaga aaaatgatta ggcgacg
//
LOCUS       DAZPEU010000138         9825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPEU010000138 DAZPEU010000000
VERSION     DAZPEU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.139"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(78..422)
                     /locus_tag="ACHU2L_003029"
     CDS             complement(78..422)
                     /locus_tag="ACHU2L_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1357787.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            554..2464
                     /locus_tag="ACHU2L_003030"
     CDS             554..2464
                     /locus_tag="ACHU2L_003030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR1357788.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2522..3217
                     /gene="tsaB"
                     /locus_tag="ACHU2L_003031"
     CDS             2522..3217
                     /gene="tsaB"
                     /locus_tag="ACHU2L_003031"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR1357789.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3257..3838
                     /gene="yeaY"
                     /locus_tag="ACHU2L_003032"
     CDS             3257..3838
                     /gene="yeaY"
                     /locus_tag="ACHU2L_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR1357790.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4044..5729
                     /gene="fadD"
                     /locus_tag="ACHU2L_003033"
     CDS             4044..5729
                     /gene="fadD"
                     /locus_tag="ACHU2L_003033"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR1357791.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5811..6926
                     /gene="rnd"
                     /locus_tag="ACHU2L_003034"
     CDS             5811..6926
                     /gene="rnd"
                     /locus_tag="ACHU2L_003034"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR1357792.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(6980..7945)
                     /gene="yeaX"
                     /locus_tag="ACHU2L_003035"
     CDS             complement(6980..7945)
                     /gene="yeaX"
                     /locus_tag="ACHU2L_003035"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR1357793.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8001..8618)
                     /gene="yeaW"
                     /locus_tag="ACHU2L_003036"
                     /pseudo
     CDS             complement(8001..8618)
                     /gene="yeaW"
                     /locus_tag="ACHU2L_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(8960..9568)
                     /locus_tag="ACHU2L_003037"
     CDS             complement(8960..9568)
                     /locus_tag="ACHU2L_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1357794.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            9738..>9825
                     /locus_tag="ACHU2L_003038"
     CDS             9738..>9825
                     /locus_tag="ACHU2L_003038"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1357795.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         2378 a         2412 c         2585 g         2450 t
ORIGIN      
        1 attctctggt cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta
       61 ttttgcttcg ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg
      121 ttcatcaaac ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca
      181 tcccacgctt tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc
      241 agaatgctcg atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg
      301 ttggcggttt gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc
      361 gtgttgttgt ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc
      421 atcattttgt cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt
      481 tgccgtcacc acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc
      541 aacagggggt agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc
      601 aggctttaag ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga
      661 aaaaggccag ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct
      721 ggctcctgcg ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca
      781 ggatcagctc tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa
      841 cgtggcgctg ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc
      901 gctggcgggg ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg
      961 gtctaatcaa acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca
     1021 ggcgtggccg ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta
     1081 taaagattgc tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt
     1141 aaaccatcat ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat
     1201 ccctgaagcg gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca
     1261 gtattttggt cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat
     1321 cgcctaccgc accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc
     1381 ccagagtgcg caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg
     1441 tgagctgtta gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga
     1501 actttgttat gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt
     1561 tgagcgcgcc acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg
     1621 ctacagctac tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag
     1681 cgtggcggat aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc
     1741 agcaacgctg tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca
     1801 ggcggagtcg ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt
     1861 gccgcgcaat ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct
     1921 gcgaccgatc atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat
     1981 gatgcgcgat ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg
     2041 ggaaaccagt aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt
     2101 ggcaaccagt agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat
     2161 tatcgacaaa ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga
     2221 ttgtcgtttg cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac
     2281 tctcaaacag ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat
     2341 ttgtgactat cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc
     2401 cgcgccacgc acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag
     2461 gtaatttgtt acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc
     2521 catgcgaatt ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga
     2581 cggtactgtc aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc
     2641 gatggtgcag gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc
     2701 ttacgggcgc ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg
     2761 gctggcgctt ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca
     2821 aggggcgtgg cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg
     2881 cgaagtttac tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac
     2941 cgaagccgta ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg
     3001 ggtgacggta gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt
     3061 tttgcgcgat ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca
     3121 gatgtttgcc gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa
     3181 caacgtcgca tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa
     3241 aaaaaggagt cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac
     3301 gtttgcgcta atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac
     3361 cacgccgcaa caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga
     3421 ggcacgcttt ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga
     3481 aattgctacc gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg
     3541 tcgcatttat gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt
     3601 tacggtagtc gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa
     3661 atttatggtg atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat
     3721 gccgcctcag ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg
     3781 cggatggggc tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt
     3841 gcttgttttt aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta
     3901 aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg
     3961 tatgatgagt taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga
     4021 acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg
     4081 ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg
     4141 tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca
     4201 agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga
     4261 aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg
     4321 gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc
     4381 ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc
     4441 acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg
     4501 gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc
     4561 gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg
     4621 gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat
     4681 acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg
     4741 cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg
     4801 tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta
     4861 tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa
     4921 aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt
     4981 tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg
     5041 gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc
     5101 tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata
     5161 ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg
     5221 atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc
     5281 aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct
     5341 ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc
     5401 gtaaaaaaga catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg
     5461 tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca
     5521 gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac
     5581 tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc
     5641 gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag
     5701 cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat
     5761 ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca
     5821 cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg
     5881 atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg
     5941 atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag
     6001 cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag
     6061 tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg
     6121 ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg
     6181 ttacgctgga caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc
     6241 agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg
     6301 tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa
     6361 tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc
     6421 agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg
     6481 cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg
     6541 cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa
     6601 tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag
     6661 atgccttacc gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta
     6721 aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc
     6781 tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga
     6841 acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca
     6901 atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc
     6961 cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac
     7021 aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat
     7081 ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata
     7141 aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg
     7201 gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt
     7261 cttcgatagc aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt
     7321 cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt
     7381 cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat
     7441 ggactttctc agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat
     7501 ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg
     7561 acaggaaagg ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa
     7621 ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct
     7681 gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac
     7741 tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat
     7801 cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg
     7861 ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta
     7921 cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga
     7981 aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg
     8041 gcgataccat gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca
     8101 cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga
     8161 tggaacacat cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg
     8221 gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg
     8281 accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa
     8341 ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg
     8401 aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg
     8461 gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca
     8521 atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg
     8581 acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt
     8641 acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg
     8701 ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa
     8761 aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata
     8821 acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc
     8881 acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt
     8941 tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag
     9001 ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg
     9061 ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat
     9121 cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct
     9181 ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga
     9241 tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt
     9301 tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg
     9361 cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc
     9421 tgataacccc atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca
     9481 ttgcctcttc aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg
     9541 catttttgct ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac
     9601 agaattcttt gatttgcact ggttaaaata aaaatgcgca tagcttttga attgagcatt
     9661 tttctgcgct atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag
     9721 agagtaagga tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg
     9781 ctgctgttcg tatgaagggg aagacactat ctcaattatc cagag
//
LOCUS       DAZPEU010000139         9824 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPEU010000139 DAZPEU010000000
VERSION     DAZPEU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.140"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            340..954
                     /gene="evgA"
                     /locus_tag="ACHU2L_003039"
     CDS             340..954
                     /gene="evgA"
                     /locus_tag="ACHU2L_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR1357796.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            959..4552
                     /gene="evgS"
                     /locus_tag="ACHU2L_003040"
     CDS             959..4552
                     /gene="evgS"
                     /locus_tag="ACHU2L_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR1357797.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4608..5753)
                     /gene="yfdE"
                     /locus_tag="ACHU2L_003041"
     CDS             complement(4608..5753)
                     /gene="yfdE"
                     /locus_tag="ACHU2L_003041"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR1357798.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5827..6771)
                     /gene="yfdV"
                     /locus_tag="ACHU2L_003042"
     CDS             complement(5827..6771)
                     /gene="yfdV"
                     /locus_tag="ACHU2L_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR1357799.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6841..8535)
                     /gene="oxc"
                     /locus_tag="ACHU2L_003043"
     CDS             complement(6841..8535)
                     /gene="oxc"
                     /locus_tag="ACHU2L_003043"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR1357800.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8589..>9824)
                     /gene="frc"
                     /locus_tag="ACHU2L_003044"
     CDS             complement(8589..>9824)
                     /gene="frc"
                     /locus_tag="ACHU2L_003044"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR1357801.1"
                     /translation="QGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTRHQL
                     RDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDHMGF
                     TWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVSAA
                     ALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDKLGY
                     LEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWENTC
                     KAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCAPVL
                     SMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTAAVL
                     QELGYSDDEIAAMKQNHAI"
BASE COUNT         2914 a         2224 c         2024 g         2662 t
ORIGIN      
        1 gcatgtgttg cacattatcc caggaacgtt ggaggcctaa tttaaatgag gtgcaaaaaa
       61 aacaacagag gactaaaccg tggcttttgc aatacaattc ttacgcctgt aggattagtg
      121 agaagactta tagtgctaac ttgaaactat aaatcatcgg tataatccct gattttattg
      181 ttgacatttc atttatgccg actatttata tggtatactt gtcgaattat cttaaaggaa
      241 actcagattt tcttattttt attgagaaaa tgagatgacg ccttatgtct gtattactac
      301 agggagaagg gagatgcttc attgcaaagg gaataatcta tgaacgcaat aattattgat
      361 gaccatcctc ttgctatcgc agcaattcgt aatttattga tcaaaaacga tattgaaatc
      421 ttagcggagt taactgaagg cggaagcgcc gttcagcggg tggaaacact taagcctgat
      481 atcgtcatca ttgatgtcga tatccccgga gttaacggta tccaggtgtt agaaacgctg
      541 aggaagcgcc aatatagcgg aattattatt atcgtctccg ctaaaaatga ccatttttac
      601 gggaaacatt gtgctgatgc tggcgctaat ggtttcgtga gtaaaaaaga aggcatgaac
      661 aatatcattg cggctattga agctgcaaaa aatggctact gctatttccc cttctctctc
      721 aaccggtttg ttggaagttt aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa
      781 gaaattagtg tcatgcggta tattcttgat ggcaaggata ataatgacat tgcggaaaaa
      841 atgttcatca gcaacaaaac tgtcagcacg tataaaagtc gcctgatgga aaaattagaa
      901 tgtaaatcac tgatggatct ttacacattc gcgcaacgta acaaaatcgg ctaatcacat
      961 gaagttttta ccctattttt ttcttctctg ttgtggtctt tggtcgacca taagtttcgc
     1021 agacgaagat tacatcgaat atcgtggcat cagtagtaac aaccgtgtca cacttgatcc
     1081 agtacgtctg agcaacaagg aattacgttg gttagcgagc aaaaaaaatc ttgtgattgc
     1141 agtacacaag tcccaaacgg ctaccttgtt gcataccgat tcgcagcaac aggttcgtgg
     1201 tattaatgct gattatttaa atcttttaaa aagagcgtta aatatcaaat taacactccg
     1261 ggaatacgca gatcatcaaa aagcaatgga cgcgcttgca gaaggtgaag tcgatatagt
     1321 gttatcacat ttagttactt cgccgcctct taataatgac attgctgcaa ccaaaccatt
     1381 gataattacc tttccggcgc tggtaaccac ccttcacgac tcaatgcgac cgcttacctc
     1441 accaaaacca gtaaatattg ctcgggtagc aaattacccc ccagacgagg taattcatca
     1501 atcatttcca aaagcaacaa ttatctcttt tacaaattta tatcaggcat tagcatccgt
     1561 ctcagctggg cacaatgatt actttattgg tagtaacatc attaccagca gtatgatttc
     1621 ccgctatttc actcactcct taaatgtagt gaaatattat aactcgccgc gtcaatataa
     1681 ttttttcttg accagaaaag aatctgtcat tcttaatgaa gtactcaata gatttgttga
     1741 tgctttaaca aatgaagttc gctatgaagt atcacaaaat tggcttgata caggaaacct
     1801 ggcctttctg aacaaaccat tagaactcac tgaacatgaa aaacagtgga ttaagcagca
     1861 tcccaattta aaggtgctgg aaaatcctta ctcgcccccc tattctatga cggatgaaaa
     1921 tggctcggtt cggggcgtta tgggggacat tcttaatatt attaccttgc aaacaggttt
     1981 aaatttttct ccgatcaccg tttcacacaa tatccatgct ggaacacaac ttagccccgg
     2041 aggatgggat ataatacctg gcgctattta tagtgaagat cgagaaaata atgttttatt
     2101 tgctgaagcc ttcataacaa cgccttacgt ttttgtcatg caaaaagcgc ctgacagtga
     2161 acaaacatta aaaaaaggaa tgaaagttgc cattccatat tattatgagc tgcattcgca
     2221 attaaaagag atgtatccgg aggttgaatg gatacaggtc gataatgcca gcgctgcatt
     2281 tcacaagatt aaggaaggtg aacttgatgc tctggtcgcg acacagctaa attcgcgtta
     2341 catgatcgat cattactatc ctaatgaact ttatcatttt cttattcctg gcgttccgaa
     2401 tgcatcgctt tcgttcgctt ttcctcgcgg agaaccggaa cttaaggata ttattaataa
     2461 agcactgaat gcaattcccc caagcgaagt tctgcgcctg acggaaaaat ggattaaaat
     2521 gcccaatgtg accattgaca catgggacct atatagcgag caattttata ttgttacgac
     2581 attatccgtt ttattagttg gcagtagcct tttatgggga ttctacctgt tacgctcagt
     2641 tcgtcgtcgt aaagtcattc agggtgattt agaaaaccaa atatcattcc gaaaagcact
     2701 ctcggattcc ttaccgaatc caacttatgt tgtaaactgg caaggtaatg tcattagtca
     2761 taatagtgct tttgaacatt atttcactgc ggattactac aaaaatgcaa tgttaccatt
     2821 agaaaacagt gattcaccct ttaaagatgt tttttctaat gcgcatgaag tcacagcaga
     2881 aacgaaagaa aatcgaacaa tatacacaca ggtatttgaa attgataatg gcatcgagaa
     2941 aagatgcatt aatcactggc atacattatg caatcttcct gcaagtgaca atgcagtata
     3001 tatttgtggt tggcaagata ttactgaaac gcgtgatcta attaatgcac tcgaggtaga
     3061 aaaaaataaa gcgataaagg ctaccgtagc aaaaagtcag tttctggcaa cgatgagtca
     3121 cgaaataaga acaccaataa gctctattat gggcttcctg gaacttctgt cgggttctgg
     3181 tcttagcaag gagcaacggg tggaggcgat ttcacttgcc tacgccaccg gacaatcact
     3241 cctcggctta attggtgaaa tccttgatgt cgacaaaatt gaatcgggta actatcaact
     3301 tcaaccacaa tgggtcgata tccctacttt agtccagaac acttgtcact ctttcggtgc
     3361 gattgctgca agcaaatcga tcgcattaag ttgcagcagt acatttcctg atcattacct
     3421 ggttaagatc gaccctcagg cgtttaagca ggtcttatca aatttactga gtaatgctct
     3481 caaatttacc accgaggggg cagtaaaaat tacgacctcc ctgggtcaca ttgatgacaa
     3541 ccacgctgtt atcaaaatga cgattatgga ttctggaagt ggattatcgc aggaagaaca
     3601 acaacaactg tttaagcgct acagccaaac aagtgcaggt cgtcagcaaa caggttctgg
     3661 tttaggctta atgatctgca aagaattaat taaaaatatg cagggcgatt tatcattaga
     3721 aagtcatcca ggcataggaa caacatttac gatcacaatc ccggtagaaa ttagccagca
     3781 agtggcgact gtcgaggcaa aagcagaaca acccatcaca ctacctgaaa agttgagcat
     3841 attaatcgcg gatgatcatc cgaccaacag gctattactc aaacgccagc taaatctatt
     3901 aggatatgat gttgatgaag ccactgatgg tgtgcaagcg ctacacaaag tcagtatgca
     3961 acattatgat ctgcttatta ctgacgttaa tatgccgaat atggatggtt ttgagttgac
     4021 tcgcaaactc cgtgagcaaa attcttcctt acccatctgg gggcttacag ccaacgcaca
     4081 ggctaacgaa cgtgaaaaag ggttaagttg cggcatgaac ttatgtttgt tcaaaccgtt
     4141 gaccctggat gtactgaaaa cacatttaag tcagttacac caggttgcgc atattgcacc
     4201 tcagtatcgc caccttgata tcgaagccct gaaaaataat acggcgaatg atctacaact
     4261 gatgcaggag attctcatga ctttccagca tgaaacgcat aaagatctac ccgctgcgtt
     4321 tcatgcacta gaagctggcg ataacagaac tttccatcag tgtattcatc gcatccacgg
     4381 tgcggctaac atcctgaatt tgcaaaagtt gattaatatt agccatcagt tagaaataac
     4441 acctgcttca gatgacagta agcctgaaat tcttcagctg ctgaactctg ttaaagaaca
     4501 cattgcagag ctggaccagg agattgctgt tttctgtcag aaaaatgact aaattgcggc
     4561 tcccacaatg ttctaatgtg ggagctattt accagcacat cttacattta tgatgagaac
     4621 tcctggcgaa tttgttcccc atgctggtcg agcgttgccg ctcccggcat aacatgcggg
     4681 tccgcgcagc cgctgatttt tatcggatta cccggcatca ttattcctcc ggcttcaatc
     4741 aacatatttc tcgcctgagt ttgtggcaaa ttaatggcct cagccacact taataacggc
     4801 gcgacgggca caccaacttc atgtattttg gctaaccaaa cttcagctgc ctgcgttttt
     4861 aacgttcgct caatatattg tttaagaata gcctggtttt gtacgcgtaa aatattgctg
     4921 ctaaatcggg gatcattaac cagttccgta agctccagtg cctggcataa cgcagaaaaa
     4981 agcttgtcat taccacaaca aatcgtaatc ggcttatcct gagtatcgaa aacatcaaaa
     5041 ggtgccatgt agggatggcg atttcccagg cgttgtggtg acttccctgt cgcaatatat
     5101 gccatcagac catgctccag aaaactcagc gtggcatcaa acatcgctat atcgacatgc
     5161 gcccctctct ggctcttttc gcggccataa agtgcactca ctattccgct gaataaatag
     5221 acaccgccgc atagatccgc aagagaggta ccaacacgca ctggcggagc atcagggtac
     5281 cctgtttcca tcattatccc actcattgcc tgaatgatgg tatcgtaggc aggagcatct
     5341 tttagcggac cggtatgtcc gaaacctgac gatgaagcat atatgaggcg cgggttgatt
     5401 tcttgtaacc tttcccatga aaaccccagt ttttccattg tacctaggcg aaaattctca
     5461 gctaatacat cagcttgttt gagcatattt ataaatatac ttttatcgtg atcattcttt
     5521 aaatcaagaa ccacactctc tttgccatga ttaataaaac tgtaatagag tgactgtcca
     5581 tccacatagg gaccaaatgt gcgggtatca tcaccatgac ctggtggctc aactttaatt
     5641 acccttgcgc ccatattata aagaagttga gttccgaaag gtccattaag gacatgtgtc
     5701 atatcgataa ctaataagcc ttcaaacggc cctttgcttt cattatttgt catttgctta
     5761 tgccttgaaa aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa tcaataacat
     5821 gattaattat gaaaccagtc gactgacata aatccacaag ggagccgtga cgacaaagcc
     5881 cagtacgctc acagccaatg acgctgtacc agtacgggtg tagacattaa accggctggc
     5941 aataatgatt ccggagaatg ccggcggtaa tgcgcctgcc agtaccatca tctgcaaatg
     6001 ttcgctgttc aaatgacatg ccataccaac gagaagcagc gccagtggca tcagaatcaa
     6061 cttcaggaag gtgttataag caatttcggc actgaactcg aatttatgtg cagccagagt
     6121 caaccccgca gcgaataccg ctacccctga gttagccttc gcaatcagat taaaggttgg
     6181 gtcccatgct gccggaattt ttaccccaac cagcaccagg atcgttgcca gaacaggtgc
     6241 ccatactact ggctcctttg ccgcagaaat taatgcgctc agattactat tcttcttacc
     6301 atccgctcct gaggaaggat tcagcaaata cagaccaata ggaatagtaa ttgcgttaac
     6361 aataatagaa ataattgcta ccactaaacc tgttgatacg gaatcaccat aaataggatc
     6421 gagaactgca aaccccagga atccaatggt aggtgaaccc gcaattaatg cacatacagc
     6481 tgcttctgca tgggtacgtt taaaaaattt gtagcaacca aaccaggaga aaaagaaaca
     6541 tccgacaata accacaagtg atacaagggt cagacgagtg tccgcaaaaa tcatttccct
     6601 gtttgcccgg gtaatagaaa caaatagagc cgcaggaaga gcgtagttta ataccagttt
     6661 attaaatgcg cgggcttgat cttctgaaaa agtttctcgt ctgccgctaa aataacccaa
     6721 cagcatgatt acgataatag gcaataaatc gccaataaaa aatgttagca taatagtatt
     6781 tccgataagg gtcccctcac catcgcataa tgagttggca agaggagacg atgtcagtat
     6841 ttaattacca gcgacttgtt ttgggttaag tttggtaata tggccacttt cagttcctgc
     6901 tgcagggtcg ataaccacat taataatggt cggtttgcgc gactgaatac cggtggttaa
     6961 agcatgacga agttcatctg tcgtggtgac gttatagcca acgccacgaa acgcatccat
     7021 taatttgtca taccttgcat ggtgcaacag atccgttggt gatggtgcac cagcgccact
     7081 gagatcaaca ccgtctcctc tgtagatgcc gccattatta aaaataacga tcgtcaccgg
     7141 caggttatat cgacaaatcg tttcaatttc catcccactg aaaccaaaag cactatcacc
     7201 ttcaatggcg acaaccggag aaccagaagt cacgctagca ccgatggcat agcccatacc
     7261 gatgcccatg acaccccagg taccacaatc cagacgacga cgtggtttat acatatcaat
     7321 aatatttcgt gcattatcca gggtatttgc accttcatta actaaataaa tatcctggtt
     7381 ctcgcgcaat acgtcgcgca cagcacttaa tgcattaaag taatttaatg gttgagtatc
     7441 tgtacttaat ttttcatgca ttttttgtgc attttgctgc ttgtggatat ttaaaatatc
     7501 gcgccatacc agtggagtcg taaatgtgtt ttgtttcagt tctgccagca taccttgcat
     7561 actggatgca atatcaccaa cgactggcac agcaatgggg cggttgctgt caatttcctg
     7621 cggttcaata tccagttgaa taaactgtgt atctgccgcc catccttttt taccgtgtgc
     7681 caataaccaa ttcagtcgtg caccaacaag catgacaacg tcagcatttg ccagggcaaa
     7741 cgaacgcgca gctgccgcag aaagtggatg cgtatcttca aggatccctt tcgccataga
     7801 cattggcagg aatggaatct gagcactttc aataaattca cgaagctgtt catcagcttg
     7861 tgaatacgcc gcgcctttgc caaggataat taatggccgt tcagcttttg ctaaaagcga
     7921 aattgcgcta gtgactgact tcgggcatgg caataatgct ggcgacggat tttcaacttt
     7981 aacaatcgtg gttaacgctt cgtctttttc catcgtcgcg gccaggacat ttgctggcaa
     8041 atcaagataa actccgccag ggcgacccga tacagagacc cggatagcgc gtgccaatgc
     8101 aatgccaaga tcctgcggct gattaacgcg aaatgctgct ttggcatacg gttttgccgc
     8161 attcatttgg tccagctctt cataatcacc ttgctgtagg tcgacgatcg cgcggtcgct
     8221 ggagccgcta atcatgataa tcggaaaacc atttaccgtt gcgttggcca atgcggtcaa
     8281 accattgagg aatcctggcg cagaaactgt caggcagatc cccggttttt gggtaagaaa
     8341 accgcttgcc gcagcggcat agcctgccga ctgctcatga cgaaaaccaa tataacgaat
     8401 gccttccgcc tgggcatggc gtgccatatc cgtcacagga atacctacaa caccataaat
     8461 agtgtcaata ttattctgtt ttaatgcttc aacgatgata tgcataccat ctgtcatttg
     8521 aagttgatct gacatgatta ataacctcga aatttttata aattgggagc cagaagcccc
     8581 cgttaatatc agatggcgtg gttttgcttc attgcagcaa tttcatcgtc gctataaccc
     8641 agctcctgca atacagcagc ggtatgttca cctaatagcg gcgcagcttt aatatccggc
     8701 gtaaaggcag agaatttcat tggacaacca acggtcagat attttccacg caacggttgt
     8761 tccacttcaa caacactgcc actttggcgc aaagagggat caagtgaaat ttctttcata
     8821 cttaaaaccg gtgcacaagg aatatcaaac tgagtcaaat aggccaccgc ttcatgttta
     8881 tcaatagtga cagtgtattt ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgg
     8941 gcatgggctg tactgtatgc cggatcggta atccattctg gtttgccgat ggctttacag
     9001 gtgttttccc agttttgctc ctgaatagtg aaataaatat aggcgttagg atcggtttcc
     9061 cagcctttac atttcaggat ccagccaggc tgaccgccac cacctgcatt accaccgcgg
     9121 ggaactgcat caccaaatgt accattcgga tactgcgggt attcttccag ataacccaat
     9181 ttatcgagac gctgctggtc acgtaatttc acgcggcaaa ggttcaatac ggcatcctgc
     9241 attgacatgg tgactcgttg cccacgcccc gttttttcgc gatgcagcaa agcagcaagt
     9301 aaaccgatca gcaaatgcat tccggtgttg ctgtcaccca acgctgcagc gcttaccagc
     9361 ggcggaccat cccaaaaacc cgtagtggat gccgcgccac ccgctgcctg agcaacgttt
     9421 tcataggctt ttacattcac ataaggcgaa cactcatcaa accctttgat cgaaccaaaa
     9481 atcagacgtg gattgatttc ttgaatatgc tcccaggtga agcccatgtg atcaatggcc
     9541 cctggatgaa agttctcgac taagatatca gcttcgcgga tcagcttttc cattacctct
     9601 ttgccttccg ctgttttggt atttaactca atagaacgtt tgttactgtt aagcatggtg
     9661 aagtaaagcg catcgatatc aggaatatct cgcagttggt gacgcgttac gtcaccaacg
     9721 ccgggacgtt caattttaat aacgtcagcg ccaaaccagg ccagcatttg agtacaagat
     9781 gggccagatt gcacaccggt gaaatcgaga actttaattc cttg
//
LOCUS       DAZPEU010000140         9774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPEU010000140 DAZPEU010000000
VERSION     DAZPEU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.141"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACHU2L_003045"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACHU2L_003045"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR1357802.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACHU2L_003046"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACHU2L_003046"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR1357803.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACHU2L_003047"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACHU2L_003047"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR1357804.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACHU2L_003048"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACHU2L_003048"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR1357805.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACHU2L_003049"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACHU2L_003049"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR1357806.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACHU2L_003050"
     CDS             complement(6031..6360)
                     /locus_tag="ACHU2L_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR1357807.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHU2L_003051"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHU2L_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR1357808.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACHU2L_003052"
     CDS             7998..8171
                     /locus_tag="ACHU2L_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR1357809.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHU2L_003053"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHU2L_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR1357810.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2492 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAZPEU010000141         9763 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPEU010000141 DAZPEU010000000
VERSION     DAZPEU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.142"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            383..1501
                     /locus_tag="ACHU2L_003054"
     CDS             383..1501
                     /locus_tag="ACHU2L_003054"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR1357811.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHU2L_003055"
     CDS             1567..1815
                     /locus_tag="ACHU2L_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR1357812.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHU2L_003056"
     CDS             1880..2248
                     /locus_tag="ACHU2L_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1357813.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFCPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHU2L_003057"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHU2L_003057"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR1357814.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHU2L_003058"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHU2L_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR1357815.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHU2L_003059"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHU2L_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR1357816.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHU2L_003060"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHU2L_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR1357817.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHU2L_003061"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHU2L_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2388 a         2705 c         2368 g         2302 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gcctttttgc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg cag
//
LOCUS       DAZPEU010000142         9680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPEU010000142 DAZPEU010000000
VERSION     DAZPEU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.143"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(44..214)
                     /locus_tag="ACHU2L_003062"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHU2L_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHU2L_003063"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHU2L_003063"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1357818.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHU2L_003064"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHU2L_003064"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1357819.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHU2L_003065"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHU2L_003065"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1357820.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHU2L_003066"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHU2L_003066"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1357821.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHU2L_003067"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHU2L_003067"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1357822.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6241
                     /locus_tag="ACHU2L_003068"
                     /pseudo
     CDS             5109..6241
                     /locus_tag="ACHU2L_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6330..6863
                     /gene="blc"
                     /locus_tag="ACHU2L_003069"
     CDS             6330..6863
                     /gene="blc"
                     /locus_tag="ACHU2L_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1357823.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHU2L_003070"
     CDS             complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHU2L_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1357824.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHU2L_003071"
     CDS             complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHU2L_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1357825.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHU2L_003072"
     CDS             complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHU2L_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1357826.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHU2L_003073"
     CDS             complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHU2L_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1357827.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8395..9423
                     /gene="epmB"
                     /locus_tag="ACHU2L_003074"
     CDS             8395..9423
                     /gene="epmB"
                     /locus_tag="ACHU2L_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1357828.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2479 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta ccccccttga
     5881 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     5941 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     6001 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     6061 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     6121 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     6181 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     6241 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     6301 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     6361 cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag
     6421 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     6481 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     6541 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     6601 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     6661 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     6721 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     6781 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     6841 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     6901 ttaacgccag actcgccaga cgcatcgggt tagcggactc accgagcagc acaatgccgg
     6961 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     7021 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acggtaataa
     7081 cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt
     7141 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga aatttggggc
     7201 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     7261 ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa
     7321 acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca
     7381 gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca
     7441 gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata
     7501 acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac
     7561 atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag
     7621 cgcgagagat ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac
     7681 agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg
     7741 acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag
     7801 agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg
     7861 gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat
     7921 cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa
     7981 cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt
     8041 tagcattgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca
     8101 tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag
     8161 cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac
     8221 gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt
     8281 caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat
     8341 agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg
     8401 catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt
     8461 gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg
     8521 gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc
     8581 atggagaaag gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag
     8641 tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct
     8701 ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta
     8761 aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt
     8821 aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc
     8881 tccggcggcg atccgctgat ggcgaaagag cacgagctgg actggttgct cacacaactg
     8941 gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg
     9001 gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg
     9061 gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa
     9121 ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat
     9181 aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat
     9241 tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa
     9301 gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg
     9361 gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag
     9421 taactgatta aacagggaag ttaaacatta aaattgcctg atgcgctacg cttatcaggc
     9481 ctacatcgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt
     9541 acgccgcatc cggcaagagc aacaaacgca agtacagcaa ccactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata attaaccttt
//
LOCUS       DAZPEU010000143         9613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPEU010000143 DAZPEU010000000
VERSION     DAZPEU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.144"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHU2L_003075"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHU2L_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR1357829.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACHU2L_003076"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACHU2L_003076"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR1357830.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACHU2L_003077"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACHU2L_003077"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR1357831.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACHU2L_003078"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACHU2L_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1357832.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACHU2L_003079"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACHU2L_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR1357833.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACHU2L_003080"
     CDS             4025..4615
                     /locus_tag="ACHU2L_003080"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR1357834.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACHU2L_003081"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACHU2L_003081"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR1357835.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACHU2L_003082"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACHU2L_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR1357836.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACHU2L_003083"
     CDS             complement(6551..6658)
                     /locus_tag="ACHU2L_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR1357837.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHU2L_003084"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHU2L_003084"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1357838.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACHU2L_003085"
     CDS             7648..7875
                     /locus_tag="ACHU2L_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR1357839.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHU2L_003086"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHU2L_003086"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR1357840.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..>9613)
                     /locus_tag="ACHU2L_003087"
     CDS             complement(9447..>9613)
                     /locus_tag="ACHU2L_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357841.1"
                     /translation="NESFSTDEDLEYGYLTNTGNHYDVYLPPELFSQAYKLKNKEMNA
                     QLDYLNRYAT"
BASE COUNT         2172 a         2510 c         2548 g         2383 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttc
//
LOCUS       DAZPEU010000144         9572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPEU010000144 DAZPEU010000000
VERSION     DAZPEU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.145"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..133
                     /locus_tag="ACHU2L_003088"
     CDS             <1..133
                     /locus_tag="ACHU2L_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1357842.1"
                     /translation="SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            230..382
                     /gene="hokE"
                     /locus_tag="ACHU2L_003089"
     CDS             230..382
                     /gene="hokE"
                     /locus_tag="ACHU2L_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1357843.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            733..885
                     /gene="hokE"
                     /locus_tag="ACHU2L_003090"
     CDS             733..885
                     /gene="hokE"
                     /locus_tag="ACHU2L_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1357844.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1007..1627)
                     /gene="entD"
                     /locus_tag="ACHU2L_003091"
     CDS             complement(1007..1627)
                     /gene="entD"
                     /locus_tag="ACHU2L_003091"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR1357845.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="ACHU2L_003092"
     CDS             complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="ACHU2L_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR1357846.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4285..5487
                     /gene="fes"
                     /locus_tag="ACHU2L_003093"
     CDS             4285..5487
                     /gene="fes"
                     /locus_tag="ACHU2L_003093"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR1357847.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5490..5708
                     /gene="ybdZ"
                     /locus_tag="ACHU2L_003094"
     CDS             5490..5708
                     /gene="ybdZ"
                     /locus_tag="ACHU2L_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR1357848.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5705..9550
                     /gene="entF"
                     /locus_tag="ACHU2L_003095"
     CDS             5705..9550
                     /gene="entF"
                     /locus_tag="ACHU2L_003095"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR1357849.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2105 a         2505 c         2630 g         2332 t
ORIGIN      
        1 cagtaatata agcgaactga aggatgctgt tacggaatat attgaatact ataacagcag
       61 aagaattagc ctgaaattaa aaggtctgac tccaattgaa tatcggaatc agacctatat
      121 gcctcgtgta taactgtcca actttttggg gtcagtacac cctggggctg ccactccaaa
      181 ccagacaatt tggatggtag tcccttcttc gcatggaggt aatataaaca tgctgacgaa
      241 atatgccctt gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggc
      301 cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct
      361 cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc
      421 aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg
      481 cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact
      541 gtataaataa acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt
      601 tccggtttca ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca
      661 atcctggggc tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga
      721 ggcaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg
      781 gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt
      841 aatattgagt ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt gtgcggggag
      901 taatccccgc atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc
      961 ccgcagtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc
     1021 acagagttat gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac
     1081 gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat
     1141 cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca agtgtcagcg
     1201 ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt
     1261 tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg
     1321 gttgacggga aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct
     1381 ccgcaggcca caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt
     1441 cccgcaaggc ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc
     1501 cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg
     1561 ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag
     1621 ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc
     1681 ttatcaggcc tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccggata
     1741 aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc tgacgttagc
     1801 atcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta taggtatacg
     1861 cgccggcacc ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg gcattacctg
     1921 cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg acattcttcg
     1981 tcacatccca ggtcgcgctc agacccacaa tgctgtaagg actgatttct ttggtttccg
     2041 ggccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg ccgtaccagg
     2101 tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt gagttcaacg
     2161 tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc ttcagcatat
     2221 aagtgatgtt attggtccac atcaccgttt cgctaactgg tacgtttaac gatccttcca
     2281 gaccttcaac cactgctttc ggcacgttat cccactgata gagatcagtg ccgactgcgt
     2341 tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta cggaaccagg
     2401 tgacgcccgc cagccagccg tcgcgtttga actccagacc aatctctttg ttgatgctgg
     2461 tttctacttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg gcatagcagc
     2521 cctgaccttt actgtagaga atgtagttcg ggttagtctg gtataggctc ggcgctttat
     2581 aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc gatatgttca
     2641 gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc cccggcgtta
     2701 cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa atttctgctt
     2761 ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg gtattcgttc
     2821 cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg ttgctgattc cactccgtac
     2881 ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg ctgtgcagca
     2941 tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta aatttccctt
     3001 cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttca tactgcaccc
     3061 agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg tagttctggc
     3121 gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca gagttggtgt
     3181 tctgggtatc gcccgcatac aggttaccct ggcggctgta acccgcttcc agttccagcg
     3241 attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat atctttattg atcacccctt
     3301 cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga tggccctggt
     3361 tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg aagctgaatt
     3421 cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct tcctctttat
     3481 gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg ccttttttgg
     3541 taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct gccggaccac
     3601 gcagaacttc aatacgttca atcatttcag gtggcaccca ggaggtatca ccacgggtat
     3661 cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc ttgccgtcaa
     3721 tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg ttattcccac
     3781 gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg atgatctccg
     3841 acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc gaaacgccag
     3901 gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt gaaacaggtg
     3961 tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc aacaaggcca
     4021 gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa ttgcaactgt
     4081 cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt gatcctgaaa
     4141 gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc attacgctat
     4201 tgcaaatgca aatagttatt aataacatta tcaatatatt tctgcaatca atgaaaaatt
     4261 gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga gagctggtgg
     4321 cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga ggtcactttc
     4381 tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt atgggtctac
     4441 atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca gcgaattgca
     4501 ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg cagctactgc
     4561 tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg cctcgaatta
     4621 cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa tccacaaagc
     4681 tggaaaggcg ggctagggca cgctgtttct gcactcgaaa tgccgcaagc gcctctgcaa
     4741 ccgggatggg attgtccgca agcgccagaa ataccagcca aagaaattat ctggaaaagt
     4801 gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgt aacagcagaa
     4861 gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat gcccgtctgg
     4921 cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta tgtgttgatc
     4981 gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga tttctggctg
     5041 gcagtacagc aagagttatt acccctggtg aaagttattg ccccttttag cgatcgcgcc
     5101 gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta tgccggactg
     5161 cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg gtggccgcat
     5221 cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga agttagcgcc
     5281 gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat gcgggccaat
     5341 caggcgctgt atgcgcaatt acaccctata aaagaatcca ttttctggcg tcaggttgac
     5401 ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggttaat cgacctctgg
     5461 caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc gatgatccac
     5521 agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg ccgcaacaat
     5581 gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaagag tcctgccagc
     5641 agtggcttga agcccactgg cgtactctga caccggcgaa tttcacccag ttgcaggagg
     5701 cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga tggcagaaaa
     5761 actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa ccggagaggt
     5821 tgatgcgcca ttactggccc gcgcggtggt tgccggatta gcgcaagcag atactttgcg
     5881 gatgcgtttt acggaagata acggcgaagt ctggcaatgg gtcgatgatg cgctgatatt
     5941 cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta ctgcgcaggc
     6001 attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac cactggtctt
     6061 tcatcaactg atacaggtgg cagataaccg ctggtactgg tatcagcgtt atcaccattt
     6121 actggtcgat ggcttcagtt ttccggccat tacccgccag atcgccaaca tttactgcgc
     6181 attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg atgtagtgga
     6241 agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat tctgggtgga
     6301 acagcgtcgc caactgccac cgcccgcgtc actttctccg gcacctttag cggggcgcag
     6361 cgcctcggca gatattttgc gtctgaaaat ggaatttacc gacggggaat tccgccagct
     6421 ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga cggctttgtg
     6481 gctggggcga ttgtgcaatc gcatggacta cgccgccgga tttatcttta tgcgtcgact
     6541 gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc tgggtattca
     6601 tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcaaccc gactggcagc
     6661 acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg tccgtgacag
     6721 cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta ctcaatatta aggtatttga
     6781 ttaccaactg gatattcctg gtgttcaggc gcaaacccat accctggcaa ccggtccggt
     6841 taatgacctt gaactggccc tgttcccgga tgaacacggt gatttgagta ttgagatcct
     6901 cgccaataaa cagcgttacg atgagccaac gttaatccag catgctgaac gcctgaaaat
     6961 gctgattgcc cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg atattatgct
     7021 gccaggtgag tatgcgcagc tggcgcagat caacgccact caggttgaga ttccagaaac
     7081 cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc cggcgctggc
     7141 agatgcgcgt tacctgttca gctatcggga aatgcacgag caggtggtgg cgctggcgaa
     7201 tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac taccgcgctc
     7261 ggtctttttg accctggcac tccatgcaat agttgaagct ggcgcggcct ggctaccgct
     7321 ggataccggc tatccggacg atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct
     7381 gttaattacc accgacgatc aactgccgcg ctttagcgat gttcccaatt taaccaacct
     7441 ttgctataac gccccgctta ccccccaggg cagtgcgccg ctgcaacttt cacaaccgca
     7501 ccacacggct tatatcatct ttacctctgg ctccaccggc aggccgaaag gggtaatggt
     7561 cgggcagacg gctatcgtca accgcctgtt gtggattcaa aatcattatc cgctcacagg
     7621 cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt gggagttttt
     7681 ctggccgttt attgccgggg ctaaactggt gatggctgaa ccggaagcgc accgcgaccc
     7741 gctcgctatg cagcaattct ttgccgaata tggcgtaacg accacgcact ttgtgccgtc
     7801 gatgctggca gcatttgttg cctcgctgac tccgcaaacc gctcgccaga gttgcgtgac
     7861 gttgaaacag gttttctgta gtggtgaggc cttaccggct gatttatgcc gcgaatggca
     7921 acagttaacc ggcgcgccgt tgcataacct gtatggcccg acggaagcgg cggtagatgt
     7981 cagctggtat ccggcttttg gcgaggaact ggcacaggtg cgcggcagca gtgtgccgat
     8041 tggttatccg gtatggaata cgggtctgcg tattcttgat gcgatgatgc atccggtgcc
     8101 gccgggtgtg gcgggtgatc tctatctcac cggtattcaa ctggcgcagg ggtatcttgg
     8161 acgacccgat ctgaccgcca gccgctttat tgccgatcct tttgctcctg gtgaacggat
     8221 gtatcgtacc ggagacgttg cccgctggct ggataacggc gcggtggagt acctcgggcg
     8281 cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa tcgatcgcgt
     8341 gatgcaggcg ctgccggatg tcaaacaagc cgttacccac gcctgtgtga ttaaccaggc
     8401 ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat ctggtatcgc aatcgggcct
     8461 gccgttggat accagcgcat tgcaggcgca gcttcgcgaa acattgccgc cgcatatggt
     8521 gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc gccaacggca agctggatcg
     8581 caaagcctta ccgttgcctg aactgaaggc acaagcgcca gggcgtgcgc cgaaagcggg
     8641 cagtgaaacg attatcgccg cggcattcgc gtcgttgctg ggttgtgacg tgcaggatgc
     8701 cgatgctgat ttctttgcgc tgggcggtca ttcgctactg gcaatgaaac tggcagcgca
     8761 gttaagtcga cagtttgctc gtcaggtgac gccggggcag gtgatggtcg cgtcaaccgt
     8821 cgccaaactg gcaacgatta ttgatggtga agaggacagc tcccggcgca tgggattcga
     8881 aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc atccggcgtc
     8941 cggttttgcc tggcagttta gcgtgctctc gcgttatctc gatccacaat ggtcgattat
     9001 tggcattcag tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc tggatgaagt
     9061 ctgcgaagcg catctggcaa cgctacttga gcaacaaccg cacggcattg cggcgcggct
     9121 gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc cgccagagac
     9181 gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg cggagattaa
     9241 ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac
     9301 caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc
     9361 gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag gtatgagccc
     9421 cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca
     9481 tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc gcgcaacgct
     9541 aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAZPEU010000145         9543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPEU010000145 DAZPEU010000000
VERSION     DAZPEU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.146"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..271)
                     /locus_tag="ACHU2L_003096"
     CDS             complement(<1..271)
                     /locus_tag="ACHU2L_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357850.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELL"
     gene            complement(264..485)
                     /gene="traR"
                     /locus_tag="ACHU2L_003097"
     CDS             complement(264..485)
                     /gene="traR"
                     /locus_tag="ACHU2L_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR1357851.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(620..1135)
                     /gene="traV"
                     /locus_tag="ACHU2L_003098"
     CDS             complement(620..1135)
                     /gene="traV"
                     /locus_tag="ACHU2L_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR1357852.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(1132..1452)
                     /locus_tag="ACHU2L_003099"
     CDS             complement(1132..1452)
                     /locus_tag="ACHU2L_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR1357853.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(1439..2005)
                     /gene="traP"
                     /locus_tag="ACHU2L_003100"
     CDS             complement(1439..2005)
                     /gene="traP"
                     /locus_tag="ACHU2L_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR1357854.1"
                     /translation="MAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDN
                     TTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAADAADYVRE
                     TDRSLMQLVTAFWATLALMYMSFAAGTGKYPVLPGKMKCIRVVTADEYLKDVYTEDAS
                     LPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(1995..3446)
                     /gene="traB"
                     /locus_tag="ACHU2L_003101"
     CDS             complement(1995..3446)
                     /gene="traB"
                     /locus_tag="ACHU2L_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR1357855.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPAPPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPLGGDAGKEGGNGG"
     gene            complement(3446..4174)
                     /gene="traK"
                     /locus_tag="ACHU2L_003102"
     CDS             complement(3446..4174)
                     /gene="traK"
                     /locus_tag="ACHU2L_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR1357856.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFVVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(4161..4727)
                     /gene="traE"
                     /locus_tag="ACHU2L_003103"
     CDS             complement(4161..4727)
                     /gene="traE"
                     /locus_tag="ACHU2L_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR1357857.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(4749..5060)
                     /gene="traL"
                     /locus_tag="ACHU2L_003104"
     CDS             complement(4749..5060)
                     /gene="traL"
                     /locus_tag="ACHU2L_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR1357858.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(5075..5434)
                     /gene="traA"
                     /locus_tag="ACHU2L_003105"
     CDS             complement(5075..5434)
                     /gene="traA"
                     /locus_tag="ACHU2L_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR1357859.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(5467..5694)
                     /gene="traY"
                     /locus_tag="ACHU2L_003106"
     CDS             complement(5467..5694)
                     /gene="traY"
                     /locus_tag="ACHU2L_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFR1357860.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLNRFPDFYNLDAIKEGAEETDSIIKDL"
     gene            complement(5831..6502)
                     /gene="traJ"
                     /locus_tag="ACHU2L_003107"
     CDS             complement(5831..6502)
                     /gene="traJ"
                     /locus_tag="ACHU2L_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFR1357861.1"
                     /translation="MYPTDPRQLNTERQIYLDKQFFVDVFSIPACVRNTNGDFIGYNE
                     KFSKEFIGSLDIKEWFYSLPVQVATSFLREELDAMSLPSSMNKIQSVAIGDKLWLVQF
                     IPLIYGEVVNVLWLFFCKNSNVIVDYCRGLRTNITNDRMLEFKNKSTEIQWKVFILYS
                     FGFCHESIASLLSITNGSSRNAISEVYKFFGIHSKHDLLMIFHTSRMHSLFFDELFFI
                     LKCAE"
     gene            complement(6696..7079)
                     /gene="traM"
                     /locus_tag="ACHU2L_003108"
     CDS             complement(6696..7079)
                     /gene="traM"
                     /locus_tag="ACHU2L_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR1357862.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            7402..8004
                     /locus_tag="ACHU2L_003109"
     CDS             7402..8004
                     /locus_tag="ACHU2L_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR1357863.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(8301..9122)
                     /locus_tag="ACHU2L_003110"
     CDS             complement(8301..9122)
                     /locus_tag="ACHU2L_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR1357864.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(9233..9529)
                     /locus_tag="ACHU2L_003111"
     CDS             complement(9233..9529)
                     /locus_tag="ACHU2L_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357865.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
BASE COUNT         2583 a         2419 c         2022 g         2519 t
ORIGIN      
        1 ggagtaattc ctgacgactg agtaccatgc gtgattcctc cggtacgctg taataattaa
       61 aaatacgact gaatacccca caacagcagg caacggacag gcacagggat ttcagataca
      121 ggccagaaac acagaatgtg gcaacaccac aaagaatagc tataactaaa aatcccttac
      181 ccagtgccat acacaaagaa ccctgttttc tggatatccg ctcatgctca tccagaataa
      241 aatctatttt ttcattttct gatttatgca tagtgtttcc tctgcctttc ctgatacgcc
      301 tgacattcaa cgcacaacgt cacaccggga aatattttcc gccgggcttc cggaatagga
      361 tttccgcatg cttcacagag ataaacagga ataccatgaa catttatttc ctggcgtatc
      421 cggtttattc ctgtcatggt cagttgttct gtcactgaat atgcttcatc ggcttcatca
      481 ctcacggaat attcctcccg acatgttctg acacgctacc gcccatccct tttcacgcac
      541 agggcactcg ttttacaggc ggattttgaa tacagaaagc ccgccggcat tgccggctga
      601 tatacaactt cagggccagt taattaatac gtggttttcc ccacgcagac ggtttaataa
      661 caaaaaacac gctggatggc tgatgataaa catcctggtt atcaatataa ggcgctatcc
      721 ataatgctgc cgtctgctca cccgttctta gcggacgagg aggtgttacc ggcgtaactg
      781 aactgaccgg aacaaccgtt ttcacttccc gggcagcggt aaacagcggg cgtggtgcca
      841 gcagtttctg ctccggatgt gctgtcaccg caggtctgct gccggacgga gtggtcgcgg
      901 tgacggtctg caccggcatt gtccggaagt tcccttcagc caggcgcggc agtgatgccg
      961 caaccggctt tgcttctgag gactgctcca gttttttggc cttctcattg gcctgctcca
     1021 tcgtcataca ggtatcggat gtggtggcgt tacactcaaa ttccgtattt gttcctgcgc
     1081 agccggaaag taataaggtt cccagcagag gaataaataa agaaatctgt ttcattgccc
     1141 ttctcctttt ttattactcc ccacaacagc agaaattaac tctgctgtca gatttgtttt
     1201 ccctgcacag ggatgtccgg agaccactgt atttcccgga gaagaaaata agctcatcga
     1261 cattcccttc ttcatcacac cgacagcagc catattaaaa ttgtgcggca atgattcaga
     1321 actgaacacc ttcggagcag gaagaactat ttttgtggtg cagttactga gaacagcatg
     1381 cataaaatca tctgatacgt ttttatccgg cacaacgcgg gctgtaataa cattaatatt
     1441 acgcatgttc atttttatct ccgttgcttc tgtttgttcg gtcatcaggg aaataaacat
     1501 ggcatttcct gatttttccc ggcagggaag cgtcttccgt ataaacgtcc ttcagatatt
     1561 catctgcggt taccacccgg atacatttca tttttccggg cagcaccgga tatttcccgg
     1621 tgcctgccgc aaacgacata tacatcagtg ccagcgttgc ccagaatgcg gtgaccagtt
     1681 gcatcagtga ccggtcagtc tccctgacat aatctgcagc atcagccgcg cggtaatggc
     1741 agatatcctg tatttctgat ctgcctgaag aatcagagga agaaaaccag cattcactga
     1801 ccgggaactg tccggcaggt gctgtttgtc ttacgaagtt aatctcctta accagtaatt
     1861 tccccggcgt ggtgttgtcc ttccataaaa caaacagagc catcagggcc attgtggcca
     1921 gcggccagat aaccacaaac ctgacgaccc acagcagcca gcgaacgaca aacgccacac
     1981 ggaatgtcag tgcatcaccc gccatttccg ccctccttgc cggcatcccc gcccagcggg
     2041 ttcttgtctc cggacataaa ctgctgcgga ttcagattgc ccagctgttt cagcatctgg
     2101 tcggtgttaa aaccgttcag gtggctttct gccgagggag gattcggcac cgggctttcc
     2161 gggttaccct cttccgctgt actctgtgac tgctccagtg ccatttcctc cacggttttc
     2221 agctggaagc cgtcctggaa caccacggtc acttcgttgc ccgcaccaat cggtatcacc
     2281 gggtgatact gttcggcacg tttgatgtag tagtcactga gcgtctgtgc ggctttcgat
     2341 gcgccgccac cgatacccat tttcaggaca tcaccagccc cgtaagcggc tgtggcaccc
     2401 agcccgacag ccggttggga ggcgcgctcc attccctgac cgataccgtc aacaaatccc
     2461 gcgccccatg cccagccgag gattttgccg ttacgcatca ccacttcgcc cttgataccg
     2521 tttttacccc ggaagctgac atgcccctta atcggcatat caatagtttt gccgtccttc
     2581 aggcaactga tattgcgggt gcgtacaatg gcacgttcac tggacacatc cccccaggct
     2641 tccagcccca caaaacatcc cgttgcgtca tacgtcttgc tgttcggcat ttccaccagg
     2701 ccggtgatac gcaactgcat cggcaccgtg gattcattac cggtgactga ggcattggca
     2761 tccgcccctt caatcagcat ggctttcgca aaacttcctg acggaatgta cggcagcgat
     2821 ggtccctgtt ttccctcatt acgggtaaac accttacgct gtatccggtt aggcaccggc
     2881 acagactggt acgtcacctg tggtggtggc gtgacaccat tcccgggata aaacgccgtc
     2941 ggcggtggaa cagcaacgct gccctgcggc gggaaggata cgggggtgtt tcctggctgt
     3001 ggttcgcctt ccgggccggg cggtggggca ggcatctgcg gtacaggctc accggtcgcc
     3061 gtgacgggat tagcacccag ggcttttacc tgctctgcca gggcggcatt gtcctgtccc
     3121 agcttttcaa tacgacgctg gtcatcaccg cgctgtttgt tcagaacatc cagctcacga
     3181 cggatttcct catactgctt ctgcatctgc gctgccgtca cctgcatctc tgtggtggca
     3241 tgctgacgca ccttgtcatc aaaggtcgta tccaccacac cggtcatatc cggcacaggc
     3301 tcctgttcag ccacggtttc accgttaccg gacatgtcca catcagacag atacagtgcc
     3361 ccaccaatcg cggaggctgt accgacaacc acaatcccca gccacaggta ctgcttgcgt
     3421 ttcacaatgg tattgatact ggccatcagt tgccctcccc gtcacggatg acatacacat
     3481 ccatgcgcgc acctgccagt aactggctgg caggctggct gaacatcacg gcacgggttc
     3541 ccggctgcca gaaatcactt tcccggatat tcagggcaga cagcgtttta ttttccacga
     3601 caaagcggac catcttcagg tgattccccg tccatacgga atctgccact gaagacagcc
     3661 ccgccggggc ctgcagggtt tcctttgtca caggaacctg ataccagcct gcaggtaatt
     3721 ttccgccacg gacagcctgg ctgatggtca caagcaggga ttcgtaaggc gtggacgttt
     3781 cccacgctcc ggcttcttct ccggttccgc gcaggtcact gaccagctga atggtacgcc
     3841 ccgcgccttc acggggaaca gcctgaatgg aaaggttcag accacgctct gtttccagaa
     3901 tgaacgtaaa gggctttttg ttgacggtgg ccaccaccac accgccggag gcggtctgct
     3961 cattattggt cagggcacca tccagactgt tcacggcgat aatccggtca ccgggaacgg
     4021 taaacagatt cgggctggta ttgctgatgg caatactggc ctgaccaccg ttcaccattg
     4081 gcaccacagt gggggccagc gtaccgtttg cggccatcac gctgcaggaa aaaaacaggc
     4141 cgccgaatat tattgccgta ttattttttc tcatcgtctg tttccccgaa attatccagc
     4201 caggtcaccc cgttttcccg cttcagaata aggatgtaat gtttgatatc agtaaaaggt
     4261 tcggagttac caatccatgt tttccgaatt ccacggattt ccacacgacc atactgaggc
     4321 cagacacgga cactggtctg gaaaaaagca gaattcacgt tatctttttt aatgagtttc
     4381 gcttcttcag ccagaataac tttcatctgg ttctgtgcac ccgggcggat atattgcaga
     4441 agcgcctgat gtgaagcatc gacgatttcc ggtgagacat tcagacggag ggcaataaat
     4501 gacagcgcca tctgctgcaa ataagaggca tcggcactgt tctgtgacac ggcaaagggc
     4561 acattaaatc ccattggggt cacggcagtg cgttgctcat tctgaagacg gtagttattc
     4621 accccctgaa tgaggttaac agagaggctg agaacaataa gcactgacat aaatataaag
     4681 gcgatggcca ttacacgact ggtacttaaa cgggcaccgt gttccatata acaatcctgg
     4741 tatcagttct atttaatcca ctgccggaaa cacgaatcgg gaacattatg aaaaataccg
     4801 cgcagcaggg ctgttggcat ataccagtaa atcaggtcac gtaaccagga actgccccgc
     4861 ccttttttca gttttttaat cccgaaataa accagaaccg ctgcaccaat accgaacaga
     4921 tatttcgatg ttgtgatacc ccagccaaca cagattgctg cggggatcag ttcatccagt
     4981 ggcaggccaa accagcggct ctggttggtc agtgtttccg ggaaacgata tttcttaagt
     5041 ttattctcgt ctcccgacat cgttttattt cctgtcagag gccaacgacg ctcataccca
     5101 cagcaataaa tacagagatg atggcaaagc cgaccaggaa cttgacgttt ttggtcatca
     5161 tgtacatgac cgcaccgacc agcacctcag ccagaacaac ccatttaacg atactggagt
     5221 ccttaccgaa ggtcgcctta accgtatcgt tacctttagc catcaggtcg cctttaccag
     5281 cagccatagc taactcaggg aacaacatca tcagaacagc aaccgggatc actgcacgaa
     5341 ccaggcgaag catattcaga cgagtgaatt tagaaaaaaa cgatttcttt tcaacgggcg
     5401 cagaagcacc ctgaacactt aaaacagtat tcataagtta cctctattgt ggatagatta
     5461 ataaacctaa agatctttaa ttatcgaatc agtttcctct gctccctcct ttattgcatc
     5521 cagattataa aagtcaggaa aacggttcag atgatcacgt aacctgacta gcacctcatt
     5581 agtttttgtt ctcccactac gttcccgggc acctaagagc ttgtgattag tagcatcgtc
     5641 caataccagt aaaactttat tgcccggagc aggccttatt atatttctgc tcaagtctcc
     5701 tcctcagcca cttagataat taaatataat tatacgaatc cataaaaata gaaacgaaaa
     5761 tagagtaaat tacattaaca aaacaaggtg acataatgtc accttgtttt taacaaaaac
     5821 gaaacatcaa ctattcagca cattttaaaa taaaaaacaa ttcatcaaag aataaactat
     5881 gcatcctgct tgtatgaaaa atcattaaca aatcatgttt tgaatgtata ccgaaaaact
     5941 tatacacttc tgaaattgca ttccgggaag aaccattagt aatagacaaa agagatgcta
     6001 tcgattcatg acaaaaacca aatgaataaa gaataaaaac tttccattgg atttccgttg
     6061 atttgttttt aaattcaagc attctgtcat ttgttatatt tgtacgtaat cctctacaat
     6121 aatccactat cacattagaa tttttgcaga aaaaaagcca taaaacatta accacctccc
     6181 catatattaa tggtataaat tgtaccagcc agagtttatc acctatagcc acgctctgta
     6241 ttttattcat ggacgaaggc aaagacatag catccagttc ttctcttaag aacgatgtag
     6301 ctacttgtac aggaagggag taaaaccact ctttgatatc aagactacct ataaattctt
     6361 tagagaattt ttcattgtag ccgataaaat ctccattagt atttcgaaca caagcaggga
     6421 ttgaaaaaac atcgacaaaa aactgtttat ccaaatatat ttgacgttca gtattaagtt
     6481 gtctaggatc cgtcggatac ataggaacct cctctcaaag gattctatgg acagtcgatg
     6541 cggggaggtc gctctccctg catcggcgat ttttaatatc tgttttaacg tggcattaac
     6601 cacataatta atataacctt tatgtggtta attgtcatca aattgaacca gatcaaaatc
     6661 ctgttatttg aagtaaatca acttttaatt cttttttatt cctcatcatt ttttggaaaa
     6721 aatcgttcca tctcagatga caccttctca cgaatatctt caaccatatt ggcatattca
     6781 aactttggat tcccggagac atggggactt agagactcaa tacccaaaat tttcgctact
     6841 gatgactgtg tttttacaac acattcaaga agaagtttat taaactctgt ctgattaaac
     6901 gcagactctt tacgctccat ctgagcctca tatacacgaa gccccagttc aagaagcatt
     6961 gaagctgttc ccgaaacact tatatcttta tctcttgccc cctcctgacg acgctgttca
     7021 actatcgcat ttactttatc atagacatca ttactgatat acaaaattac tctggccata
     7081 agataaaacc tttcattatt aagcaatgaa cttttcacta taaatatgca tatagtgttt
     7141 acaagtaaga aagacactcc tagcagcgcc tctaggatca tcctataaaa aaatgcgatc
     7201 cggcgctagg ggcgtcccta atatatatca atgtttttca tgaaaattgt cagtactgat
     7261 cctaataaga gtcgctatag ggtcgtaaca ggatcgccaa cgactctcta tttaataatt
     7321 cagaattatt aaatataaat agcgtttgtt aattacatga tttaaaacgt aaatcagcaa
     7381 aaacttgttt ttgcgtagtg tgtggtgctt ttggtggtga gaaccaccaa cctgttgagc
     7441 ctttttgtgg agtgggttaa attatttacg gataaagtca ccagaggtgg aaaaatgaaa
     7501 aaatggatgt tagcaatctg cctgatgttt ataaatggga tctgcgaagc cgccgattgc
     7561 tttgatcttg caggtcggga ttacaaaata gacccggatt tactgagagc gatatcatgg
     7621 aaagaatccc gttaccgggt taatgccatc ggtattaatc cggtaacggg atatggcagc
     7681 ggactgatgc aggtagattc ccagcatttt aacgaactgg cccgctatgg aattaagccg
     7741 gaacatctga caacagatcc ctgtatgaac atttataccg gtgcttatta tctggcaata
     7801 gcctttaaaa aatggggcgt ctcctgggag gccgttggtg catacaatgc cggattcagg
     7861 aagaccgaac gccagaacca gagacgtctt gcctacgcat cagaggttta ccggatttat
     7921 acctggataa agagcagtaa aggcatccgg gttccgacca cgaagaaatc actttcccaa
     7981 attaacagtg tgcagaaaaa ttaatattgt atacattgtc aaataccggt tatgccgcga
     8041 cagcggcatg gaaggccaca cacaaaaata tcatatgagt gataatgctg actggccgaa
     8101 gccccgcaaa gcgggatccc atcttatata tatccggtaa ctgcgcagcc tcctgccaga
     8161 ccatttttcg cgggcacaat atttacagac taccgaaaag ccagttgatg aaattgaggc
     8221 atatcagacg acacagcaat gccgcaggat gataatataa gtaaagcccc gaaaaatttt
     8281 tcggggcttt acttatatta tcatgacttc cactccctga actgttcggc aatcatccat
     8341 aatgctttgt tgattcggat atcaccgtca atgcctgtaa tggctcttgt tctggtattt
     8401 ttaccggagg cacttcgccc cgataatcca ccttttatca tattctcctg aacccgctgc
     8461 caggttgtcc agagatcgtt ctgtttatcc tcccaacggc ggggagtaat tatttgttct
     8521 ggcgtcactg gcgttttatt ttcatcttcg tacctggcca tcaatgcagc tctgccaaat
     8581 aaatgttgtt cgtcactgtt aagatgtatc tctttcattg tctccatatt ttcagtgact
     8641 ttatcaaaga caccgagcac ttcatacgct ccctcaataa cctgcccgac aatatcacct
     8701 ttatgtggaa cgcggatttc gccaaaatta ttcccacaca ccaggccatt tgtgcagaca
     8761 aaacgaaaaa ttcctgggat catctgataa ctggatgaac catcatgtga attaagcagg
     8821 ataatttcag gaacttcttt cccgttaata tgcccttccc tgcgaagacg taacatatgt
     8881 ttactgtatt cgcggcgtcc caaatcacga acccgactct gacaggcaaa gaatggctgg
     8941 aaaccttcat cacgtaactt attgatgata ttgatggttg ggatatatgt ataacgctca
     9001 ctccgggatt catgtttatc accagaaaag acagatggaa caaactgcat taattcgtca
     9061 tccgttaacg ggcgctcacg gcgaatagaa ttatatcgac caaaacggga agctaatcgc
     9121 ataacagtaa ctcctttatg aaggcataaa attaataaaa gttttttcgc ttaatatcat
     9181 cccccctcgg ttatcgttaa caaaaagaca acaattccga ggggaaggac catcaaatca
     9241 ggtcagttcg ggactgacca acctgccgag tgaaaagcag taacagcgcg atcaactcat
     9301 cagcgttttt tctgacaaga ggagtatgat tctccaggct atccagtaag tcatgcagct
     9361 cattattgac ctggtggagc accttaaaga gtgcaccaga atcccggcag ctctgatatg
     9421 caagatttgc ctgaacagca cgattctgca tgatcccgat ttgtgcgatt gcgtctttct
     9481 gctgatcgcc tgttggctga taagtggtgt taaaagaaaa tgcgctcata attcctcctg
     9541 tat
//
LOCUS       DAZPEU010000146         9537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPEU010000146 DAZPEU010000000
VERSION     DAZPEU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.147"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHU2L_003112"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHU2L_003112"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR1357866.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHU2L_003113"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHU2L_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR1357867.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHU2L_003114"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHU2L_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /gene="actS"
                     /locus_tag="ACHU2L_003115"
     CDS             4131..4886
                     /gene="actS"
                     /locus_tag="ACHU2L_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR1357868.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="ACHU2L_003116"
     tRNA            4965..5038
                     /locus_tag="ACHU2L_003116"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="ACHU2L_003117"
     CDS             5173..6783
                     /locus_tag="ACHU2L_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1357869.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7234
                     /locus_tag="ACHU2L_003118"
     CDS             6815..7234
                     /locus_tag="ACHU2L_003118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357870.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8353..9099
                     /locus_tag="ACHU2L_003119"
     CDS             8353..9099
                     /locus_tag="ACHU2L_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1357871.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9397..>9537
                     /locus_tag="ACHU2L_003120"
     CDS             9397..>9537
                     /locus_tag="ACHU2L_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357872.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2422 g         2321 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccg
//
LOCUS       DAZPEU010000147         9523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPEU010000147 DAZPEU010000000
VERSION     DAZPEU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.148"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(52..954)
                     /locus_tag="ACHU2L_003121"
     CDS             complement(52..954)
                     /locus_tag="ACHU2L_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR1357873.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1092..2333)
                     /gene="yihS"
                     /locus_tag="ACHU2L_003122"
     CDS             complement(1092..2333)
                     /gene="yihS"
                     /locus_tag="ACHU2L_003122"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR1357874.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2350..3228)
                     /gene="yihT"
                     /locus_tag="ACHU2L_003123"
     CDS             complement(2350..3228)
                     /gene="yihT"
                     /locus_tag="ACHU2L_003123"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFR1357875.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3252..4148)
                     /gene="yihU"
                     /locus_tag="ACHU2L_003124"
     CDS             complement(3252..4148)
                     /gene="yihU"
                     /locus_tag="ACHU2L_003124"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR1357876.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4316..5212
                     /gene="yihV"
                     /locus_tag="ACHU2L_003125"
     CDS             4316..5212
                     /gene="yihV"
                     /locus_tag="ACHU2L_003125"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR1357877.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5246..6031
                     /locus_tag="ACHU2L_003126"
     CDS             5246..6031
                     /locus_tag="ACHU2L_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1357878.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6130..6729
                     /gene="yihX"
                     /locus_tag="ACHU2L_003127"
     CDS             6130..6729
                     /gene="yihX"
                     /locus_tag="ACHU2L_003127"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR1357879.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6723..7595
                     /locus_tag="ACHU2L_003128"
     CDS             6723..7595
                     /locus_tag="ACHU2L_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR1357880.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7592..8029
                     /gene="dtd"
                     /locus_tag="ACHU2L_003129"
     CDS             7592..8029
                     /gene="dtd"
                     /locus_tag="ACHU2L_003129"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR1357881.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8074..9015
                     /gene="fabY"
                     /locus_tag="ACHU2L_003130"
                     /gene_synonym="yiiD"
     CDS             8074..9015
                     /gene="fabY"
                     /locus_tag="ACHU2L_003130"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR1357882.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            9431..>9523
                     /locus_tag="ACHU2L_003131"
     CDS             9431..>9523
                     /locus_tag="ACHU2L_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357883.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQT"
BASE COUNT         2249 a         2375 c         2522 g         2377 t
ORIGIN      
        1 ctttagcgag tgaaatgttc ttcaatgatt ttgactgcta atcaatacaa gttattttct
       61 ctgtccgtaa atattaaaaa acaggcggtg cggttttcct ggttccagta ataataagtc
      121 gatacctgaa ttaaaggcgt taggcgggca actcataggt tcaactgcca gtccctgacg
      181 ctgtaatttt tcaccgctat aaacctgcac ccagggttga tcggaacaaa ggcttaccga
      241 aagcgcctgc tgtggatggg taatggtcat ttcccacaaa tcattagcgg ttttaaaggt
      301 gtggtcgatt tttgtcgcgg ctattttttt cgcctgcgtg aaatttaaat ccagctcatc
      361 aacgtggtga agcgttgtcg gatttacgtg ttcatcgacg gcataaacct ggtttgccgg
      421 aagctggaac agatattcat caacagatgt taagttgcag gttaaataag gatgaatacc
      481 cacgccataa ggcgctgcga cagtgccgat attctggctt gcgatctcaa cggaaagacc
      541 cgtgtgtgcg ttgagcgaat aaacaacttg tgaagccagc ataaacggat agccgtaact
      601 gggcggcaga aaagctgtca gggtaacgct ggtggtggtt aattcactaa tttgccagtc
      661 tcgccaggca agcagcccgt gaatagcggc ttttgaacta tgttcattaa tgggtaactg
      721 atattcctgc ccctgataac ggtaacagcc gttggcgatc cggttcggcc acggaataag
      781 cactttaccc agatgcgcca gcggcatctc ttcgggtttg tgcgggatga ctaaatgaca
      841 accctgaaag gttaattcag ccagaccagc accgacggtc acaatggtcg cgtgataatc
      901 ccccgcggca agtgaaacgg tctgtccact gcaatgcata ttggtgattt gcatacaagg
      961 caccttaatt aacgacatac agcctcccgt gaggctgatg tcaaacccaa aaactgcacc
     1021 ggacggtccc ctcgcccctt tggggagagg gttagggtga ggggaaaacc cgcaccctat
     1081 tttcagatca gttatttcgc attaatatcc agcagacccg ctgcaaccgc tggagccatg
     1141 ccgggcgcta acgggatacg cgggatcacc aggcaatgca gcaggtgata aatatcctgt
     1201 ttgccgtccc agactttggt gatgacctta ttgtccgcat ccagctcctg ccaccaggaa
     1261 ccattttcat agtccatcag gtacttaatg cagtactccc accatgtttg ataccaggtt
     1321 tcatactggc gatcgccagt gacggtgtag agcgcgtaga ccgtacccat tgcttcgacg
     1381 ataggccaac gtacacgttc gcggaccacc ggttttcctt cccagtcaac ggtataaaca
     1441 atcccgtccg ctccatcggg tgcccaggca tcgcgcacgg tggcgttaaa cagacctttg
     1501 gcatcttcta gcagccatgc tggtggttgt tcgcaacggg cttccagggc agcatggatg
     1561 tgcagcatta aacggcccca ttcgatccag tggcctggtg tacctccgaa cgcgcggaag
     1621 cgatgcgccg ggttatcttt gttgtaatcc ggcagcggat tccactgggt atcgaagtgt
     1681 tcgttaacgc gataatgatt atttctggcg acgtcgtgga taatcacgga agccacgcga
     1741 atcgcgcgat ccagccattt tttgtcgtga gtgacgtcat aaacaatcaa gaaagcttcc
     1801 accgcgtgca tattggcatt gccgccgcgg tactcttcgg ttttgctgaa ggcttcgtcc
     1861 caggactcca ggcacatctg ctcttcttcg ctccagaaat atttctcgat aatctcaatg
     1921 gtgtaatcga gcagcttgcg cgcttccggg tgacccgttg tgacggcgct ggcggcaccc
     1981 agcagagcaa agaagtgttg atagccctgt ttggaggcat ccaccacgcc ctcgtcattc
     2041 acgcaggcat accagccgcc atatttttta tcacgcagtg cgccgttcat ggctttaata
     2101 ccgtgatcaa ccaacgcgta agcgccaggt cgacccatcg ccgcagcaac ggaataaacg
     2161 tgcaacatac gagctgtgat ccacagatgg gtgcccatct cttctttgat ttgcccttta
     2221 ttgcctaacc agccaaaacc agtcggcact acggaatttt taccaaaatc aaagatgcgg
     2281 tcggtttcct gttccagcca acggttgtgg cttagggtgt taaaccattt cattcctttt
     2341 atcctcatct tagcggcgtt tagccatcat ttcgtcgacg atatcgccaa gttgttgtaa
     2401 tttcggtgcg caaacgtcac ggagcatcag ctcgttgtct ggtaaaccga cgaccgatgc
     2461 ccagactgca cggcctgcca ggaatcccga tgcgcccgcc gtcattgcca cgcgtacggc
     2521 acgcgggaac agtttttcgt cgacactgga agagaggatc acccatggca tattgatatg
     2581 gtcattcaga cgttgtgaag cacagagaag ctcttgttgc ggacctttgc cataaagcgg
     2641 catttcaact ttgtagaggt cagcgccact gtcgcccagc tctttggcgg catcgatgat
     2701 cgcttgttcg cgatcgaatt tatcgccacg acgcggtggg cggacgactg gctcaatgat
     2761 gcttaccaga ccgtgtgagt ggcacagttc gttgaactct ttcaccatat ccagacgttg
     2821 ctgcgcatct tcatcactac gccacagcac cagcagtttt aaggctttgc caccgtcttg
     2881 tttgatctgt agcggattga ttttgcggtc aataaccacg ctatcgaccg gaataccgtt
     2941 gccaggaatg aactcatcgg cggcgacaat catggcgcaa cttttggcaa tcgcgttttg
     3001 ctcaaccacc tggcggtagc agaattgttg atctaccaga atcgccgagg cataaggcga
     3061 gagggccttt gcagcgttaa ctttgaaatc agttaaaacg ctatcggcta ccggagcggg
     3121 tgccccagcc gcggcaaaca tcatgcgcat ggcttcgcgc tgatcgaccg ccagcatggc
     3181 aaaaccgccc gatgcgcgcg taatgtcgtt gatggtgtac ttattcattc atttattcct
     3241 ttatccagtc gttacatttt tactttggca gtcatcccgg cactgacacg gacctgttcc
     3301 agaatggcgg accagtcctg gcgaccgcga cccgctgcgc gcgcctggct ataaacctcc
     3361 cgtgaggcgg cccccagcgg cattggcaca tgcagctggt tggcgacatc aagggcgatg
     3421 ccaagatcct tatgggcaag atcgatcatg aaggcgggag aaagatctcc gctgaggact
     3481 ttgtttggcc aggaagtggt gaagtgacct ttaccggcgg cggtaccgct catcactttg
     3541 acggcaacat cgaagggaag attcagggct tcgcacaaaa cggcggcttc tgccgaaagc
     3601 gcattgagcg cgatgctcat gtagttgttg atgagcttaa cgcggatccc catgcccgga
     3661 ccgccagcgt tgatcaactc actgcccatc gccatcagga tcggtgtggc acgttcaact
     3721 tgttcagcgg tgccgccagc cagcagtaac agagtaccgg taatggcatt tgcagaagta
     3781 cggcctaccg gaacatccat catgctgaag cctttggctt gcatatcggc aatcaattta
     3841 tcggtttgca gcggatggat ggtggacata tcaatgacca gcgcatcggt agataagcct
     3901 tcgcaaacgc cgttttcacc gaacaacacg ttgcgcacca gatcgccatt cggcaacatg
     3961 gtaatgataa attcggcatc tttagcggcc tgcgccgggt tggcggcggg agtcgcacct
     4021 ttgtctacca gatgccgcac agcttcggcg ttcacatcaa agacgcgaag ttgatgccct
     4081 tgctgcaata aattgctcgc cattggcgaa cccatttgtc ctaaaccgat aaacgcgatt
     4141 gctgccataa ccctctcctg aatacagtga tgtcactttt tgtcatttat gacatgcttt
     4201 gcttgtctgt ttttgatcga atttgtaatt tatcgtcaaa aaattgacag ccgtcacttt
     4261 ttaaacaatt ggtgaaatta aaatgaacgc atcccaaaat gtttaaggaa tgaccatgat
     4321 tcgtgttgct tgtgtaggta taaccgtgat ggatcgcatc tattacgtgg aagggttacc
     4381 gacggagagc ggtaaatacg tggcgagaaa ttatacggaa gttggtggcg ggccagcggc
     4441 gacggcagcg gttgcggcgg caaggctggg ggcgcaggtc gattttattg gtcgcgtagg
     4501 tgatgacgac gcgggcaaca gcctgctggc agagctggaa tcctggggcg ttaacacccg
     4561 ttacaccaaa cggtataacc aggcgaaatc ttcgcaatcc gccatcatgg tggataccaa
     4621 aggcgagcgg ataatcatta actaccccag cccggatctg ctgcctgacg cagagtggtt
     4681 ggaggaaatt gatttctctc agtgggatgt tgtgctggca gatgtacgct ggcacgacgg
     4741 cgctaaaaaa gccttcaccc tggcccgtca ggcgggtgtg atgaccgttc tggacgggga
     4801 cattacgccg caggatatca gtgagctggt ggcattaagc gatcacgcgg ccttttcaga
     4861 accgggtctg gcgcgcttaa cgggcgtgaa agagatggcc agtgcgctaa aacaggcaca
     4921 aacgctcaca aatggtcatg tctatgtgac ccagggtagc gcaggctgcg actggctgga
     4981 aaatggtggg cgtcagcatc aaccggcctt caaagttgat gtggtagata ccacaggtgc
     5041 gggagatgtt tttcacggcg ctttggcggt ggcgctggca acaagtgggg atttagcgga
     5101 gtcagtccgc ttcgccagcg gtgtagcggc gttaaaatgc acacgtcccg gtggacgagc
     5161 cgggatccct gactgtgatc aaacccgatc ttttttgtca ctttttgtat aaaatgccag
     5221 ggtgatggtt tttcgaggaa ttttcatgag ccttaccgaa ctaaccggta acccgcggca
     5281 cgaccaactc ctcatgctga tcgccgagcg tgggtatatg aatattgatg agctggcaaa
     5341 tctgctggat gtctccacgc agacggtccg ccgggatatt cgtaaattaa gcgagcaagg
     5401 cctgattacg cgccatcacg gtggcgcggg tcgggcttcc agcgtcgtta atacggcgtt
     5461 cgagcagcgt gaggtttcgc aaaccgagaa aaaaaaagcg attgccgaag cggtggcaga
     5521 ctatattcct gatggttcaa caatatttat caccattggt acgactgttg agcatgttgc
     5581 ccgggcgtta cttaaccata atcatttgcg gataatcacc aacagcctgc gtgtggcgca
     5641 tattctttac cacaacccgc gctttgaagt gatggtgccc ggcggtacgt tgcgctctca
     5701 taatagcggg atcattggcc cttcagcggc gtcctttgtg gctgattttc gtgctgatta
     5761 tctggtaaca agcgttgggg cgattgagag cgatggcgcg ttgatggagt ttgatgtaaa
     5821 cgaagctaac gtggtgaaaa cgatgatggc gcacgcgaga aatattctgc tggtcgccga
     5881 tcacactaag tatcatgctt cggcggcggt tgaaattggt aacgtggcac aggtcactgc
     5941 gctctttacc gacgagctgc cgcccgctgc gctaaaatca cgcttacaag acagccaaat
     6001 tgaaatcatt cttccccagg aagacgcgta gatttttgtg accgttaacc tggcttcata
     6061 ccttgccaca tagccaaacc catcctttcc cgctacagtt aatttcttgt ggcgcgaaag
     6121 gaggcaaaaa tgctctatat ctttgattta ggtaatgtga ttgtcgatat cgactttaac
     6181 cgtgtgctgg gagcctggag cgatttaacg cgtattccgc tggcatcgct taagaagagt
     6241 tttcatatgg gggaggcgtt tcatcagcat gagcgtgggg aaattagcga cgaagcgttc
     6301 gcagaggcgc tgtgtcatga gatggctcta ccgctaagct acgagcagtt ctctcacggc
     6361 tggcaggcgg tgtttgttgc gctgcgcccg gaagtgatcg ccatcatgca taaactgcgt
     6421 gagcaggggc atcgcgtggt ggtgctttcc aataccaacc gcctgcatac caccttctgg
     6481 ccggaagaat acccggaaat tcgtgatgct gctgaccata tctatctgtc gcaagatctg
     6541 gggatgcgca aacctgaagc acgaatttac cagcatgttt tgcaggcgga aggtttttca
     6601 cccagcgata cggtcttttt cgacgataac gccgataata tagaaggagc taatcagcta
     6661 ggcattacca gtattctggt gaaagataaa accaccatcc cggactattt cgcgaaggtg
     6721 ttatgctaaa aaccattcag gacaaagcca ggcaccgtac ccgtccacta tgggcctggc
     6781 taaaactact ctggcaacgt attgatgagg acaacatgac aaccctggca ggtaaccttg
     6841 cctatgtgtc gttgctctca ttagtgccgc tggttgccgt tgtttttgcg cttttcgccg
     6901 cttttcccat gttttccgac gtcagcattc agttacgtca ctttattttt gccaactttc
     6961 tgcctgctac tggcgatgtt attcagcggt atatcgaaca atttgttgct aattccaaca
     7021 agatgaccgc cgttggggcg tgcgggctga tcgtcaccgc gttattgttg atgtactcca
     7081 tcgatagcgc gttgaatacc atctggcgca gtaaacgagc gcgaccaaaa atttactcgt
     7141 tcgccgtgta ctggatgatt ttaacgctgg ggccgctgct ggcaggggcc agtctggcga
     7201 tcagttccta tttgctctcc ctgcgctggg cgagcgatct caatactgtc atcgacaacg
     7261 tgctgcgtat ttttccgttg ctgttgtcgt ggatctcctt ctggttgctg tacagcattg
     7321 ttcctaccat ccgcgtacct aaccgcgacg cgattgtcgg cgcgtttgtc gccgcactcc
     7381 tgttcgaagc aggaaagaaa ggtttcgcgc tttatatcac catgttcccg tcatatcagc
     7441 tcatttacgg tgtgctggcg gtgatcccca ttctctttgt ttgggtctac tggacatggt
     7501 gtatcgtctt gcttggcgcg gaaattactg tcactctcgg ggaataccgc aaactaaaac
     7561 aagcagctga acaagaagaa gacgacgaac catgattgca ttaattcaac gcgtaacccg
     7621 tgccagcgtc accgtggagg gagaagtgac gggcgaaatt ggcgcgggac ttttggtgtt
     7681 attgggtgtc gaaaaggatg acgacgaaca gaaagcaaac cgtctgtgcg agcgtgtgct
     7741 cggctaccgc atctttagcg atgccgaagg caagatgaat ctcaacgtgc aacaggcggg
     7801 cggcagtgta ctggtggttt cccagtttac cctcgccgca gataccgaac gggggatgcg
     7861 cccaagtttc tccaaaggcg catcaccgga tcgcgcagag gcgttatatg actatttcgt
     7921 cgaacgctgc cgccagcaag agatgaacac gcaaacagga cgcttcgctg ccgatatgca
     7981 ggtatcgctg gtcaatgatg gccccgtgac attctggttg caggtatgag ccagcttcca
     8041 gggttgtcac gggaaacaag agagagtatc gctatgtatc accttcaggt tccacaaaca
     8101 gaagaagagt tagagcgtta ctatcacttt cgctgggaaa tgttgcgtaa gcccctgcat
     8161 caaccaaaag gttcggaacg cgacgcgtgg gatgcgatgg cgcatcacca gatggtcgtc
     8221 gacgagcagg gtaatctggt ggcggtaggc cgattgtata ttaatgccga caatgaagcg
     8281 tccattcgct ttatggtcgt tcacccggac gtgcaggaca aagggttagg aacgctgatg
     8341 gcgatgaccc tggagtcggt ggcgcgtcag gaaggcgtta agcgcgtgac ctgtagcgcc
     8401 cgtgaagacg cggtggagtt tttcgccaag ctggggtttg ttaatcaggg agaaatcacc
     8461 acgccaacca ccacgccgat tcgccatttt ttgatgatta agcccgtcgc cactctggat
     8521 gatattttgc atcgcggcga ctggtgcgcg cagctgcaac aggcgtggta cgaacatatc
     8581 ccgcttagtg aaaaaatggg cgtgcgtatt caacaatata ccgggcaaaa atttatcacc
     8641 accatgccgg aaaccggcaa tcagaatccg caccatacgc tgtttgccgg gagtttattc
     8701 tcgctggcga cgctcaccgg ttgggggctt atctggctga tgctgcgtga acgccacctc
     8761 ggcggaacga ttattctggc ggatgcgcat atccgctaca gcaaaccgat tagcggtaaa
     8821 ccccatgcgg tagccgacct tggtgcctta agcggcgatc tcgaccgtct ggcgcgcgga
     8881 cgaaaagcac gggtgcagat gcaggtcgaa atctttggcg acgagacgcc gggtgcagtg
     8941 tttgaaggca cgtatatcgt tctgcccgcg aagccatttg gcccgtatga agagggcggg
     9001 aacgaagaag agtagcttat ggagaggggc ggtgctgcct ctctcattca ttccggtcat
     9061 ttacatcagt ccctgactat tccggttata ttgaataaga actttataaa tttttattct
     9121 tcgttggcac catacgtgtc ttgctacatc actgttcagg agcatttaaa tattatttgt
     9181 agagctgagt ggcaattatt gcagataatg cttaactcat catattagtt caggtttgag
     9241 cgatttttga tagtcaaaac cccctctggt gccatacttt tttcataaat atttttaaaa
     9301 tctatagctc acaaatatta tgcaaagata atgggctggc atacattgtc agtatcattc
     9361 atttactgct ctgtatgtta tgaatcatta tgttgtatgt ttgctcttaa accgtgggaa
     9421 ggaggctata atggttgcca atattaatct cattaaacaa gaaagttatt ctgttgttaa
     9481 tttagaaaaa caactaatga gtaataaatc accgaagcaa acg
//
LOCUS       DAZPEU010000148         9512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPEU010000148 DAZPEU010000000
VERSION     DAZPEU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.149"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..276
                     /locus_tag="ACHU2L_003132"
     CDS             <1..276
                     /locus_tag="ACHU2L_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1357884.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            432..1967
                     /gene="gadC"
                     /locus_tag="ACHU2L_003133"
     CDS             432..1967
                     /gene="gadC"
                     /locus_tag="ACHU2L_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR1357885.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2098..3417
                     /locus_tag="ACHU2L_003134"
     CDS             2098..3417
                     /locus_tag="ACHU2L_003134"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR1357886.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3794..4864
                     /gene="dosC"
                     /locus_tag="ACHU2L_003135"
                     /pseudo
     CDS             3794..4864
                     /gene="dosC"
                     /locus_tag="ACHU2L_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            4864..5481
                     /locus_tag="ACHU2L_003136"
                     /pseudo
     CDS             4864..5481
                     /locus_tag="ACHU2L_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5739..6071
                     /gene="ddpX"
                     /locus_tag="ACHU2L_003137"
                     /pseudo
     CDS             5739..6071
                     /gene="ddpX"
                     /locus_tag="ACHU2L_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6085..6981
                     /gene="ddpC"
                     /locus_tag="ACHU2L_003138"
     CDS             6085..6981
                     /gene="ddpC"
                     /locus_tag="ACHU2L_003138"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR1357887.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6978..7952
                     /locus_tag="ACHU2L_003139"
     CDS             6978..7952
                     /locus_tag="ACHU2L_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1357888.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7945..8871
                     /locus_tag="ACHU2L_003140"
                     /pseudo
     CDS             7945..8871
                     /locus_tag="ACHU2L_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="ACHU2L_003141"
     CDS             complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="ACHU2L_003141"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR1357889.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2320 a         2331 c         2389 g         2472 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgccc agcagaacag ctttaaacat acctgataac gtttaacggt aacggtgtcc
      301 cgaaacgaac ccgtttcggg ataatttcca aagtctgttc actggcatta gcaacggaaa
      361 atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga ataccgtttt
      421 agggggataa tatggctaca tcagtacaga caggtaaagc taagcagctc acattactcg
      481 gattctttgc cataacggca tcgatggtaa tggctgttta tgaataccct accttcgcaa
      541 catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg tttattcccg
      601 tgggactttg tgctgcggaa atggccaccg tcgacggctg ggaagaaggt ggtgtcttcg
      661 cctgggtatc aaatactctg ggtccgagat ggggatttgc agcgatctca tttggctatc
      721 tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca ctctcctaca
      781 tcctgaaatg gccagcgctg aatgaagacc ccattaccaa aactattgca gcactcatca
      841 ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg cgaattgcta
      901 aagttggctt cttcgccggt atcctgttac ctgcatttat tttgatcgca ttagcggcta
      961 tttacctgca ctccggtgcc cccgttgcta tcgaaatgga ttcgaagacc ttcttccctg
     1021 acttctctaa agtgggcaca ctggttgtat ttgttgcctt cattttgagt tatatgggcg
     1081 tagaagcttc cgcaactcac gtcaatgaaa tgagcaaccc cgggcgcgac tatccactgg
     1141 ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt ttgtctattg
     1201 cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa acctttaccg
     1261 ttctgatgtc ccatgtggca ccggaaattg agtggacggt tcgcgtgatc tccgcactgc
     1321 tgttgctggg tgttctggcg gaaatcgcct cctggattgt tggtccttct cgcgggatgt
     1381 atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac aaaaatggcg
     1441 taccggtaac gctggtcatt tcgcagctgg tgattacttc tatcgcgttg atcatcctca
     1501 ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg acggtggtga
     1561 tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt cttaaacatc
     1621 ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg gtcgtggcaa
     1681 ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg ccggataaca
     1741 tccagggtga ttctaccgat atgtatgttg aattactggt tgttagtttc ctggtggtac
     1801 ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aggcaaagcg aataccggcg
     1861 tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc ctgcacccgc
     1921 gtgcacgttc accacactat attgtgatga atgacaagaa acactaacca tgtctgaatg
     1981 gttataagac aagggagcga taattcatcg ctcccttttt cgtgcttgct gtcgtattga
     2041 cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaacc agcacatatg
     2101 gatatctgct cccgaaacaa gaaattaacg attagaagac cagcgatact ggttgcattg
     2161 gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac accaccagca
     2221 ggttcaaagc caccagccac gacgcaacaa tcgtcacaac cgatgcgtgg catctggctg
     2281 gccacggttt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag taatcccacc
     2341 agccgagccc gtgtacaaca acaggcgatg atcgacaaac tggatcatct ccaacgtctc
     2401 ggcataaaca cggtcttttt ccaggtcaag ccggacggta ccgccctgtg gccatcgaaa
     2461 attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg ttacgatccg
     2521 ctgcaattca tgctcgacga agcccacaag cgtgggatga aagtacacgc ctggtttaac
     2581 ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa tagcactctg
     2641 tctcaacaac cggcgagcgt ctatgtgcaa caccgtgact ggatcagaac gtctggcgat
     2701 cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc aatagtcgct
     2761 gaagtggttt cccgctatcc ggtagatggc gtgcagtttg acgactattt ctatactgaa
     2821 tcaccgggtt cacggctaaa tgataacgaa acgtaccgta aatacggagg cgcatttgcg
     2881 tcaaaagcag actggcggcg caacaatact cagcagttaa ttgcaaaggt atcacacacc
     2941 attaaaagca ttaagccggg agtcgaattt ggcgttagcc cggcaggcgt gtggcgtaac
     3001 cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga cgaatcctac
     3061 gcagacaccc gtcgatgggt ggaacaagga ttgctggatt acattgctcc ccaaatttac
     3121 tggccgttct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg ggcggatgtc
     3181 gttaaaccaa ccaggacccg cctgtatatc ggtatcgcct tctataaagt gggtgaacct
     3241 tcaaagatag agccagactg gatgattaac ggcggcgtac cggaactgaa aaagcagctc
     3301 gatcttaacg atgcggtacc agaaattagc ggcaccatct tgttccgtga ggactatctg
     3361 aataaaccgc agactcaaca agcggtcagc tatctgcaaa gtcgttgggg cagttaaact
     3421 accatgctta ctttgtaaca agccggtgct ttacccaccg gcttgttaca ccttgtgaaa
     3481 tatcccacta gtaatcatgc ttacataagt caaattaacc acgcaattaa ccacacttag
     3541 ttaaacagct ataaatccct aataaaaatg tatgaatatg aaaaaccata cgatattcgc
     3601 tgtcaccgaa aaataattac gatgggctat actcttctga agaacagtag cgtagattga
     3661 aactgtctta agtcgcttat ttcttttcag tatatcttca tatttcagga gaatatggtg
     3721 cagttagtcg atctcgcacg ttgcgtttcg ttttgattcc gtctgatttc ccagccttat
     3781 aagggtgtga attatggaga tgtattttaa aagaatgaaa gatgagtgga ccggacttgt
     3841 cgaacaggca gatcctctca ttcgtgctaa agccgcagaa attgcccttg cgcatgctca
     3901 ttatctgagt atcgagtttt atcgaattgt ccgcatcgac ccgcatgccg aagaattctt
     3961 gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt
     4021 gctttctgcc caggttgacg atgtcgaaag gctaatacaa atccagcata ccgtcgcgga
     4081 agtgcatgcc cgcataggaa ttccggtaga aattgtcgag atggggtttc gggtgctgaa
     4141 aaaaatcctc tatccggtaa tcttctcttc ggattattcc gccgcagaaa aacttcaggt
     4201 ctaccatttc tcgattaaca gtattgatat cgcgatggaa gtgatgaccc gcgcgtttac
     4261 ctttagtgac agtagtgcct caaaggaaga tgaaaactat cgtatcttct cgttactgga
     4321 aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg aaatagatat
     4381 tatctataaa gtcctgctgg attctgattt aggcagcagt ttgcctttaa gccaggctga
     4441 ttttggcctg tggtttaacc ataaaggtcg acattatttt agcggcattg ccgaagtggg
     4501 ccatatctcc cgtctgattc aggatttcga cggtattttc aatcaaacca tgcgtaacac
     4561 caggattttg aataacagaa gtttgcgggt gaaatttcta ttacagataa gaaataccgt
     4621 atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg
     4681 tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct tcaaacgcga
     4741 aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg acgttgataa
     4801 attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc tgcgtaaagt
     4861 ctcattcctc tcgcagaaga gattggtgaa atcgaaaata ttgggcgctg ggtcatcgcg
     4921 gaagcttgcc gtcagttagc agaatggcgt agccagaata ttcatatccc ggcgttatcc
     4981 gtgaacttgt cggcgctgca ctttcgcagt aatcagctgc ctaatcaggt gtctgatgca
     5041 atgcaagcct ggggtattga cggccaccag ctgacggtgg aaatcacgga aagcatgatg
     5101 atggaacacg ataccgaaat ctttaagcgc attcagatcc tgcgtgatat gggcgtgggt
     5161 ttatcggtag atgattttgg cacgggcttt tccggattat cccgcttagt cagtcttccg
     5221 gtaacggaaa tcaaaattga caaaagcttt gtcgatcgtt gtctgacgga aaaacgcatc
     5281 ctggccttac ttgaagccat taccagcatt gggcaaagcc tcaatttaac cgtcgtggcg
     5341 gaaggggtcg aaaccaaaga gcaatttgag atgctacgta agatccactg tcgcgttatt
     5401 cagggatatt tcttttcccg cccccttccc gccgaagaaa ttccaggctg gatgagcagc
     5461 gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca ctcgcgggtt ttcctttaag
     5521 gtgacactgt cacttaaata ctgtgatttc agctacgatt ccgggagatt ccttccttaa
     5581 ctttccctct tgcaccaaaa ctgcactacg ctgcacaaat gttgatcaaa agttaaaaaa
     5641 tataacttta agccagcatt ctggcgctta tcccggcatg gcatgagatc tgcataagcg
     5701 gaaagcgcag caatttttgt cttatacagg aaccgtttat gtcggatacc accgaactgg
     5761 ttgatttagc cgtgatcttc cctgatctgg agatcgaatt gaaatatgcc tgcgttgata
     5821 acatcacagg taaagctatt tatcagcaag cgcgttgtct gctacataaa gatgcgatta
     5881 ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg cttacaactg gtgatttacg
     5941 atgcgtatcg cccacaacaa gcacaggcga tgttgtggca agcctgccca gacccgcaat
     6001 atgttgttga tgtgacggtc ggttctaatc acagccgtgg cacggcgctc gacctgacgc
     6061 ttcgtgatga cgtggaggtg gtgaatgatg ctaagcgagg aaacgtccgc cgtacgccca
     6121 caaaaacaaa cgcgatttaa cggtgcaaaa ctggtatgga tgctgaaagg cagtccgctc
     6181 accgtgaccg gcgcagtcat cattgtatta atgcgactga tgatgatttt ttcaccgtgg
     6241 ctggcgacgc atgatcccaa cgccattgat ttaaccgccc gccttttgcc gccttctgcg
     6301 gcgcactggt ttggcaccga tgaagtggga cgcgatctgt ttagccgcgt actggtcggc
     6361 agtcagcaat caattctcgc cggattagtg gtggtcgcca ttgcgggtat gattggttcg
     6421 ctactcggat gtctatccgg tgtgcttggc ggacgcgcag acgccatcat catgcgcatc
     6481 atggacatta tgctgtcgat tccttcgttg gtactgacaa tggcactggc tgccgctctc
     6541 gggccgagtt tatttaacgc catgctggcg attgctattg tgcgaattcc cttttatgtg
     6601 cgcttggcgc gggggcaagc attagttgta cgccagtata cctatgttca ggcggcgaaa
     6661 acctttggcg cgtctcgttg gcatctgatc aactggcata ttttacgtaa ctccctaccg
     6721 ccgctgatcg tgcaggcatc gctggatatc ggtagcgcga ttttaatggc cgccacgttg
     6781 ggatttattg gcctgggtgc tcaacaaccg agtgctgaat ggggcgcgat ggtagcgaat
     6841 ggtcgcaact atgtactcga tcagtggtgg tattgcgcat ttccgggggc agcgattttg
     6901 cttacctccg tcgggtttaa tctctttggt gatggtattc gcgatctgct tgacccgaaa
     6961 gcaggaggaa agcagtcatg acccaacccg ttctggacat tcaacagctg catttgagtt
     7021 tccccggttt taacggtgat gttcacgcgc tcaacaatgt gtccttgcag attaaccgcg
     7081 gcgaaattgt cggtctggtg ggagaatccg gctcaggtaa atcagtcacc gcaatgctga
     7141 ttatgcgtct gctaccgacg ggcagttatt gcgtacatcg ggggcagatt tcactgctgg
     7201 gagaagatgt tcttaacgct cgggaaaaac agcttcgtca gtggcgcggc gcacgagtgg
     7261 cgatgatctt tcaggaaccg atgaccgccc ttaatccgac acgtcgaata ggtcttcaga
     7321 tgatggacgt aatccgccat catcaaccaa taagtcgtcg ggaagccaga gctaaagcga
     7381 ttgcactgct ggaagagatg caaatcccgg atgccgtgga agttatgtcg cgctatccgt
     7441 ttgagctttc aggtggtatg cgccagcggg taatgattgc gccggcattc tcctgcgagc
     7501 cgcaattgat tattgccggc gaaccgacga cggcgctgga cgtcacagta cagttgcagg
     7561 tactgcgttt gcttaaacat aaagcacgcg ccagtggaac tgcggtactg ttcatcagcc
     7621 atgatatggc cgtggtgtcg caactgtgcg atagcgttta cgtgatgtat gccggaagcg
     7681 taattgaaag cggcgggacg gcagacgtta tccatcatcc ccggcatccg tataccattg
     7741 gtttgctgca atgcgcacca gaacatggag taccacgcca gccattaccc gccattccag
     7801 ggacggtacc aaacctcacc catttgcctg acggctgcgc ttttcgcgat cgttgctatg
     7861 cggcaggtgc acagtgtgcg ctgacagcgt gtggtgacaa caaccagcac tgcacctgtt
     7921 ggtatccgca gcaggaggtc ataagtgtct gacacgttat taacgttacg cgacgtccat
     7981 atcaattttc cggcccgtaa aaactggctt ggcaaatcta cggaacatgt tcatgccatt
     8041 aatggtattg atttacagat ccgccgtggt gaaaccttag ggatcgtcgg cgagtcaggc
     8101 tgcggcaaaa gcaccctcgc acagctttta atgggcatgc tgcaaccgag ccacgggcag
     8161 tacatccgtt caggctcaca acgcattatg cagatggtgt ttcaggaccc gctctcttcg
     8221 cttaatccgc gcttaccggt gtggcgcatc atcacagaat cgctctggat agctaagcgt
     8281 agtagtgaac aacagcggcg agcgttggca gaggagttgg ctgtgcaggt gggtattcgt
     8341 ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg ggcagcggca acgcatcgcc
     8401 attgccagag cactctcttc gcagcctgac gtgattgtgc ttgatgagcc aacctcggcg
     8461 ctggatatct ccgtgcaggc gcagatcctc aatttgctgg taacgctaca ggaaaatcac
     8521 gggctgactt atgtgctgat ttcacacaat gtctcggtga tacgtcatat gagcgatcgg
     8581 gtggcggtga tgtatctcgg gcagattgta gaactgggcg acgcgcaaca ggtgttgact
     8641 gcttcggcac atccatacac ccgattattg ctggattccc tccccgccat tgataaaccg
     8701 ctggaggaag aatgagcatt acgtaaaacg gatctgccgg gaaaccgcac gttgccgcaa
     8761 ggctgttttt tctacgaacg ttgccctctg gcaacccacg gatgtgaagt ccggcaatca
     8821 ttagcaataa gggaggacgg acgtgacctc cggtgctggc gggcgctgta gagatgctaa
     8881 caacgcatca ggcattcacg cgcctgacgc gtcatccggc aatgctttac gatttcaact
     8941 ggtaatccag cgtaatttcc gctttcagta cctgagagac cgggcatcct gcttttgctt
     9001 tctggatgat gccgtcaaag gtagaggcat caatacccgg caccgcaact tcactcttca
     9061 gtgcgatttt cgtaatcgca aaaccggcat ccactttatc cagcgacaca tcggcggtgg
     9121 tatcaattga tgttggcgtg aatcccgctt cccccagcat taatgaaagc gccattgaga
     9181 aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa
     9241 aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc gctctcggtg gatactgttc
     9301 ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg attgtcattg
     9361 ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaacag cacgaggata
     9421 agcaggatat tccgaataaa atacggcgaa aatattactt ctgcacttaa attaaattcc
     9481 cacaatagat aaccgggaat atatatctca ag
//
LOCUS       DAZPEU010000149         9456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPEU010000149 DAZPEU010000000
VERSION     DAZPEU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.150"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHU2L_003142"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHU2L_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHU2L_003143"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHU2L_003143"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR1357890.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHU2L_003144"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHU2L_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHU2L_003145"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHU2L_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR1357891.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHU2L_003146"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHU2L_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR1357892.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHU2L_003147"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHU2L_003147"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR1357893.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHU2L_003148"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHU2L_003148"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR1357894.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHU2L_003149"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHU2L_003149"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR1357895.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHU2L_003150"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHU2L_003150"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR1357896.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2298 a         2477 c         2472 g         2209 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaataatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gataca
//
LOCUS       DAZPEU010000150         9429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPEU010000150 DAZPEU010000000
VERSION     DAZPEU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.151"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            129..1826
                     /gene="maeA"
                     /locus_tag="ACHU2L_003151"
     CDS             129..1826
                     /gene="maeA"
                     /locus_tag="ACHU2L_003151"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1357897.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1960..2970
                     /gene="adhP"
                     /locus_tag="ACHU2L_003152"
     CDS             1960..2970
                     /gene="adhP"
                     /locus_tag="ACHU2L_003152"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR1357898.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3116..3400
                     /locus_tag="ACHU2L_003153"
     CDS             3116..3400
                     /locus_tag="ACHU2L_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1357899.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="ACHU2L_003154"
     CDS             complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="ACHU2L_003154"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR1357900.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="ACHU2L_003155"
     CDS             complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="ACHU2L_003155"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR1357901.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="ACHU2L_003156"
     CDS             complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="ACHU2L_003156"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7631..7633),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1357902.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQSHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8450..9331
                     /gene="yddG"
                     /locus_tag="ACHU2L_003157"
     CDS             8450..9331
                     /gene="yddG"
                     /locus_tag="ACHU2L_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR1357903.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2257 a         2488 c         2391 g         2293 t
ORIGIN      
        1 gacgattaaa caccattgcc tgcgcaatgg tgtttttatc tgctttatac ttggggacga
       61 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      121 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      181 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      241 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      301 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      361 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
      421 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
      481 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
      541 ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac ggtgaacgta
      601 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
      661 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
      721 gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
      781 tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
      841 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
      901 accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact gcggcggtaa
      961 cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
     1021 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
     1081 cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg gtcgatcgct
     1141 ttggcctgct gacggacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga
     1201 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg ctggatgtgg
     1261 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
     1321 aagagatcat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta
     1381 acccgacgtc tcgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg
     1441 cgctggtcgc cactggcagc ccgtttaatc ctgtggtatg gaaagataaa atctacccta
     1501 tcgcccagtg taacaacgcc tttattttcc cgggcatcgg cctgggtgtt attgcttccg
     1561 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacgctt gctcagtatt
     1621 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
     1681 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtca
     1741 aaacgtctgc tgaagctttg caacaagcca ttgacgataa tttctggcaa gccgaatacc
     1801 gcgactaccg ccgtacctcc atctaagcct gcacccggta gtgaaggcta ccgggctatt
     1861 tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
     1921 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaagactgc agttgttacg
     1981 aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
     2041 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
     2101 tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
     2161 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttttacgaa
     2221 ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa
     2281 aatgccggat acagcgttga tggcgggatg gaggaagagt gcatcgtggt cgccgattac
     2341 gcggtaaaag tgccagatgg tctggactcg gcggcgccca gcagcattac ctgtgcgggg
     2401 gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg gattgctatc
     2461 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
     2521 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatggatgca
     2581 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
     2641 ggcgctcacg ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
     2701 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
     2761 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
     2821 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
     2881 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
     2941 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgactcc catgttcggg
     3001 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
     3061 gaacaataat gatagtgttc ccagagaagt aacgctactg agagtaaaga cactaatgaa
     3121 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
     3181 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
     3241 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
     3301 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
     3361 ggtggttgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cctataaagc
     3421 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
     3481 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
     3541 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
     3601 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
     3661 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
     3721 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
     3781 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
     3841 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
     3901 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
     3961 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
     4021 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
     4081 tgcgacgaaa atcgcaatgc cgaagaaggg gtgcaaaatg cgtcccatct gcggcgtacc
     4141 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
     4201 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
     4261 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
     4321 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
     4381 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
     4441 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
     4501 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
     4561 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
     4621 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
     4681 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
     4741 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
     4801 gaatgattgc accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac
     4861 agccgtcttt acggatcagc cactccagct tgccattctg ttcggtttcg ctaaagcgca
     4921 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
     4981 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
     5041 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
     5101 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
     5161 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
     5221 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
     5281 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
     5341 gcggtttcag acggcgcgtt accactgcca ccgcgcggat aaagcctcgc ttgctggaga
     5401 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc ctgcgtttcg ctgatttcca
     5461 caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag gtgtggaagt
     5521 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
     5581 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
     5641 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
     5701 tgttgatggc aaacaggcgt cccatccctt ccggctgcat aataaacggc ccggttggcg
     5761 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
     5821 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
     5881 tgtagagtac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
     5941 cggacgggtc ggagttatcg cggtttgcca tctggttacc ctgctctgtc cagctaccgg
     6001 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
     6061 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccaacgcat
     6121 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
     6181 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac aactcgcgca
     6241 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
     6301 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcctcttcag
     6361 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
     6421 tcgactcgcc gtggctctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
     6481 ccatgtactt cagtttgctc aggcagctca ccactttgtt tttatccggg aaggacgcaa
     6541 ccgggttaaa gccctggcag aaataaccgg taactttgcc ttcatccatc atgttgaaat
     6601 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
     6661 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
     6721 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
     6781 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
     6841 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
     6901 caccggccat acccatgtta ccgagcagcg actggatcat cgccatagta cggatgttct
     6961 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
     7021 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
     7081 agatgttttc aacaacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
     7141 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
     7201 agttccagga cgatttgtcg tactggcgtt tttcagcgtc ataaccgctg aacaggccat
     7261 cttcgaaagc aaaatcatca cgcaccagta ggctggcgtt ggtgtaatgt ttaacgtatt
     7321 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaat gccagacagg aacgtaatgt
     7381 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg
     7441 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
     7501 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
     7561 tatccaccca gtggttggtc atagcaccgc gaccaaatgt tggagcaaga cttgctaccg
     7621 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
     7681 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
     7741 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
     7801 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
     7861 tgtcagaacc tggcgcacga tatttcgggt agcgcagacg gttttcactg ttgacgtaat
     7921 ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga tccgggtcac
     7981 cttcaatgtg ataaatcgct tctctggcgt tttttgcgcc atcacccagg ctatacatca
     8041 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
     8101 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
     8161 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
     8221 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
     8281 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
     8341 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
     8401 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
     8461 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
     8521 tcgcggtgtc agcgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag
     8581 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
     8641 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tatctggcgc tttccttagg
     8701 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
     8761 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
     8821 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca
     8881 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
     8941 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
     9001 cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact attttctcac
     9061 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
     9121 taccttagga tttgcttata ctgcatggaa tgtcggtata ttgcatggca atgtcaccat
     9181 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
     9241 cagcgccccg ctgtcgttct cgttctggca aggcgcacta atggtctgcg gcggttccct
     9301 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
     9361 taatcagata aattccgaca atattaaaaa ataaatttca aataccagta attcacctta
     9421 tatctcatt
//
LOCUS       DAZPEU010000151         9375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPEU010000151 DAZPEU010000000
VERSION     DAZPEU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.152"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHU2L_003158"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHU2L_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR1357904.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHU2L_003159"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHU2L_003159"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR1357905.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHU2L_003160"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHU2L_003160"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR1357906.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHU2L_003161"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHU2L_003161"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR1357907.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHU2L_003162"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHU2L_003162"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1357908.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHU2L_003163"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHU2L_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR1357909.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHU2L_003164"
     CDS             complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHU2L_003164"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR1357910.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHU2L_003165"
     CDS             complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHU2L_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR1357911.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHU2L_003166"
     CDS             complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHU2L_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR1357912.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHU2L_003167"
     CDS             complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHU2L_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR1357913.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9182..>9375)
                     /locus_tag="ACHU2L_003168"
     CDS             complement(9182..>9375)
                     /locus_tag="ACHU2L_003168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357914.1"
                     /translation="EQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAA
                     AIARVQAKKAAQQKVVNED"
BASE COUNT         2394 a         2349 c         2383 g         2249 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tctttttttt acatcccata
     5581 ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg ttatagtggg
     5641 aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct ttgtaataat
     5701 ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat ccagcgttaa
     5761 atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga tgatggcttg
     5821 gcggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt ttctgaaaaa
     5881 tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta aatcatttta
     5941 acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc acgaatgaat
     6001 agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg caaacggatt
     6061 acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt taaaagagga
     6121 taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat gtcaactttc
     6181 tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt gcgggcaatg
     6241 caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa ctctgccgga
     6301 accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc aaattgagta
     6361 cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg
     6421 tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag cgcagcacga
     6481 tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt gatgatattt
     6541 tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc ttcaacgcct
     6601 agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt cgccttatta
     6661 acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa
     6721 ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt gaggatctcc
     6781 agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat gcacgttcgg
     6841 cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt cctgccagca
     6901 tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg
     6961 tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca tctgcaccgt
     7021 caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac atggcgcagg
     7081 ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct tttttggcgg
     7141 cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga ataaaaatcc
     7201 ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca gcgctgacca
     7261 ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc cagtgacgca
     7321 gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca
     7381 gagcgttagt ggcagtggat gtgaccgcca atagcggaca aaggccgagc agttggacca
     7441 gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg ctcacgcttt
     7501 attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg
     7561 cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg aactggtcga
     7621 aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg attttttttc
     7681 ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca agccctggcg
     7741 tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg gctcccacca
     7801 gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg gcggctaccg
     7861 gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac tctggcgcag
     7921 ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc agcacctgat
     7981 caaataacgt cttttgttgc agactggcct gttcagcaat tgtcgttttg gtcatctggt
     8041 tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta atgccgtgtt
     8101 ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt
     8161 agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag
     8221 gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg ccgaacatca
     8281 gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag aatgcgccga
     8341 gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct ggtgagaaca
     8401 accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga atatgccagc
     8461 gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga tttacccatt
     8521 gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc tgttcaaccg
     8581 aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga ctaatgccat
     8641 caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg ctgaaaataa
     8701 cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc
     8761 tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt gccggattaa
     8821 acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca aacaccgtac
     8881 ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc aataagcctg
     8941 tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt agtttgagta
     9001 cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg agagtacccc
     9061 aaccaaaaaa ccgcagttgc gctgcgattc ctggcacggc tgcgagcaac accaacagca
     9121 taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt ctgaatacca
     9181 tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg cgggcaatag
     9241 ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca ttagcctgtt
     9301 gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggca gctttgcgcg
     9361 gatcaacctg ttctt
//
LOCUS       DAZPEU010000152         9343 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPEU010000152 DAZPEU010000000
VERSION     DAZPEU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.153"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            282..2675
                     /gene="malP"
                     /locus_tag="ACHU2L_003169"
     CDS             282..2675
                     /gene="malP"
                     /locus_tag="ACHU2L_003169"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR1357915.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2685..4769
                     /gene="malQ"
                     /locus_tag="ACHU2L_003170"
     CDS             2685..4769
                     /gene="malQ"
                     /locus_tag="ACHU2L_003170"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR1357916.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4814..6130)
                     /gene="gntT"
                     /locus_tag="ACHU2L_003171"
     CDS             complement(4814..6130)
                     /gene="gntT"
                     /locus_tag="ACHU2L_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1357917.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6491..7066)
                     /gene="nfuA"
                     /locus_tag="ACHU2L_003172"
     CDS             complement(6491..7066)
                     /gene="nfuA"
                     /locus_tag="ACHU2L_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR1357918.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7125..7808)
                     /gene="gntX"
                     /locus_tag="ACHU2L_003173"
     CDS             complement(7125..7808)
                     /gene="gntX"
                     /locus_tag="ACHU2L_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR1357919.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7846..8634
                     /gene="bioH"
                     /locus_tag="ACHU2L_003174"
     CDS             7846..8634
                     /gene="bioH"
                     /locus_tag="ACHU2L_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR1357920.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            complement(8635..9000)
                     /locus_tag="ACHU2L_003175"
     CDS             complement(8635..9000)
                     /locus_tag="ACHU2L_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1357921.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(8994..9263)
                     /locus_tag="ACHU2L_003176"
     CDS             complement(8994..9263)
                     /locus_tag="ACHU2L_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR1357922.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
BASE COUNT         2290 a         2321 c         2588 g         2144 t
ORIGIN      
        1 actgtttcac tcctttactt aatttataga gttaccttcc gctttttgaa aatacgcaac
       61 ggccattttt tgcacttaga tacagatttt ctgcgctgta tggcattgat ttgctgctaa
      121 tcctgtggtt tgcactagct ttaagtggtc gagatcacat ttccttgctc atccccgcaa
      181 ctcctccctg cctaatcccc cgcaggatga ggaaggtcaa catcgagcct ggcaaactag
      241 cgataacgtt gtgttgaaaa tctaagaaaa gtggaactcc tatgtcacaa cctattttta
      301 acgataagca atttcaggaa gtgctttcac gtcagtggca gcgttatggc ttaaattctg
      361 cggctgaaat gactcctcgc cagtggtggc tggcagtgag tgaagcactg gccgaaatgc
      421 tgcgtgctca gccattcgcc aaaccggtgg cgaatcagcg acatgttaac tacatctcaa
      481 tggagttttt gattggtcgt ctgacgggca acaacctgtt gaatctcggc tggtatcagg
      541 atgtacagga ttcgttgaag gcttatgaca tcaacctgac tgacctgctg gaagaagaga
      601 tcgacccggc gctgggtaac ggtggtctgg gacgtctggc ggcgtgcttc ctcgactcaa
      661 tggcaactgt cggtcagtct gcgacgggtt acggtctgaa ctatcaatat ggtttgttcc
      721 gccagtcttt tgtcgatggc aaacaggttg aagcgccgga tgactggcat cgcagtaact
      781 acccgtggtt ccgccacaac gaagcactgg atgtgcaggt agggattggc ggtaaagtga
      841 tgaaagacgg acgctgggag ccggagttta ccattaccgg tcaagcgtgg gatctccccg
      901 ttgtcggcta tcgtaatggc gtggcgcagc cgctgcgtct gtggcaggcg acgcatgcgc
      961 atccgtttga tctaactaaa tttaacgacg gtgatttcct gcgcgccgaa cagcaaggca
     1021 tcaatgcgga aaaactgacc aaagttctct atccaaacga caaccatact gccggtaaaa
     1081 agctgcgcct gatgcagcaa tacttccagt gtgcctgttc ggtagcggat attttgcgtc
     1141 gccatcatct ggcggggcgt aaactgcacg aactggcgga ttacgaagtt attcagctga
     1201 acgataccca tccaactatc gcgattccag aactgctgcg cgtgctgacc gatgagcacc
     1261 agatgagctg ggatgacgcc tgggccatca ccagcaaaac tttcgcttac accaaccata
     1321 ccctgatgcc agaagcgctg gaacgctggg atgtgaaact ggtgaaaggc ttactgccgc
     1381 gccatatgca gattattaac gaaattaata ctcgctttaa aacgctggtg gagaaaacct
     1441 ggccgggcga tgaaaaagtg tgggccaaac tggcggtggt gcacgataaa caagtgcata
     1501 tggcgaacct gtgtgtggtt ggcggtttcg cggtgaacgg tgttgcggcg ctgcactcgg
     1561 atctggtggt gaaagatctg ttcccggaat atcaccagct atggccgaac aaattccata
     1621 acgttaccaa cggtattacc ccacgtcgct ggatcaaaca gtgcaaccct gcactggcgg
     1681 ctctgttgga taaatcactg caaaaagagt gggctaacga tctcgatcag ctgatcaatc
     1741 tggaaaaatt cgctgatgat gcgaaattcc gtcagcaata tcgcgagatc aagcaggcga
     1801 ataaagtctg tctggcggag tttgtgaaag ttcgtaccgg tattgagatc aatccacagg
     1861 cgattttcga tattcagatc aaacgtctgc atgagtacaa acgccagcac ctgaatctgc
     1921 tgcatattct ggcgttgtac aaagaaattc gtgaaaacct gcaggctgat cgcgtaccgc
     1981 gcgtcttcct cttcggcgcg aaagcggcac cgggctacta cctggcgaag aatattatct
     2041 ttgcgatcaa caaagtggct gacgtgatca acaacgatcc actggttggc gataagttga
     2101 aggtggtgtt cctgccggat tattgcgttt cggcggcgga aaaactgatc ccggcggcgg
     2161 atatctccga acaaatttcg actgcaggta aagaagcttc cggtaccggc aatatgaaac
     2221 tggcgctcaa tggtgcgctt actgtcggta cgctggatgg tgcgaacgtt gaaatcgccg
     2281 agaaagtcgg tgaagaaaat atctttattt ttggtcatac cgtggaacaa gtgaaggcta
     2341 ttctggccaa aggctacgac ccggtgaaat ggcggaagaa agataaggtg ctggacgcag
     2401 tattgaaaga gctggaaagc ggtaaataca gcgacggcga taagcatgcc ttcgaccaga
     2461 tgctgcacag tatcggcaaa cagggcggcg atccgtatct ggtgatggcg gatttcgcag
     2521 cctatgttga ggcacaaaag caggtggatg tgctgtaccg cgaccaggag gcctggactc
     2581 gcgcggcgat cctcaatacc gcccgctgcg gtatgtttag ctcggatcgc tctattcgcg
     2641 attatcaggc tcgtatctgg caggcaaaac gctaaggaag ctcgatggaa agcaaacgtc
     2701 tggataatgc cgcgctggcg gcggggatta gccccaatta catcaatgcc cacggtaaac
     2761 cgcagtcgat tagcgccgaa accaaacggc gtttgcttga cgcgatgcat caacgtaccg
     2821 ccacgaaagt ggcggtaacg ccagtcccga atgtcatggt ttataccagc ggcaaaaaaa
     2881 tgccgatggt ggtggagggc agcggcgaat atagctggca gctgaccacc gaagaaggaa
     2941 cgcagtacaa aggccatgta acggggggca aagcgttcaa tctaccgacg aagctgccgg
     3001 aaggttatca cacgctaaca ctcacccagg acgaccagcg cgcgcattgc cgggtgattg
     3061 tcgccccgaa acgttgttac gaaccgcagg ctttgctgaa taaacaaaag ctgtggggtg
     3121 cctgcgttca gctttatacg ctgcgatcgg aaaaaaactg gggtattggg gattttggcg
     3181 atctcaaagc gatgctggtg gatgtggcaa aacgtggcgg gtcgttcatt ggcctgaacc
     3241 cgattcatgc gctctatccg gcaaatccgg agagcgccag cccatacagc ccgtcttctc
     3301 gccgttggct gaatgtgatt tatatcgacg ttaacgccgt tgaagatttc catcttagcg
     3361 aagaggctca ggcctggtgg cagttgccga ccacgcaaca gacgctgcaa caggcgcgcg
     3421 atgccgactg ggtcgattac tccacggtta ccgccctaaa aatgacagca ttacgaatgg
     3481 cgtggaaagg tttcgcgcaa cgtgatgatg agcagatggc cgcgtttcgc cagtttgttg
     3541 cagagcaggg cgacagcctg ttctggcagg ctgcctttga tgcgctacat gcccagcaag
     3601 tgaaagagga cgaaatgcgc tggggctggc ctgcatggcc agagatgtat aagaacgtgg
     3661 attcaccaga agtgcgtcag ttctgcgaag aacatcgtga tgacgtcgat ttttatctct
     3721 ggttgcagtg gctggcttac agccagtttg ccgcctgctg ggagataagc cagggctatg
     3781 aaatgccgat tggcttgtat cgtgatctgg cggttggcgt agcggaaggt ggggcggaaa
     3841 cctggtgtga ccgtgaacta tattgcctga aagcatcggt tggcgcgccg ccggatatcc
     3901 tcggcccgtt ggggcagaac tggggattac cgccaatgga cccgcatatc atcaccgcgc
     3961 gtgcctatga accgtttatc gagctgttgc gtgccaatat gcaaaactgc ggcgcattac
     4021 gaattgacca tgtgatgtcg atgctgcgtt tgtggtggat accgtatggc gagacggcag
     4081 atcagggcgc gtatgttcac tatccggtgg atgatctgct ctcgattctg gcactcgaaa
     4141 gtaaacgtca tcgctgtatg gtgattggtg aagatctcgg taccgtaccg gtagagattg
     4201 ttggtaagct gcgcagcagc ggtgtgtact cttacaaagt gctctatttc gaaaacgacc
     4261 acgagaagac gttccgtgca ccgaaagcgt atccggagca gtcgatggcg gttgcggcga
     4321 cacatgacct gccaacgctg cgtggttact gggagagcgg ggatctaacg ctgggcaaaa
     4381 ccctggggct gtatccggat gaagtggtac tgcgcggtct gtatcaggat cgcgaactgg
     4441 cgaagcaagg gctgctggat gcactgcata aatatggttg tctgccaaaa cgtgccgggc
     4501 ataaggcatc gttgatgtcg atgacgccga cgctgaaccg tggtttgcag cgctacattg
     4561 ccgacagtaa cagtgctctg ttaggactac agccggaaga ctggctggat atggccgaac
     4621 cggtgaatat tcctggcacc agttaccagt ataaaaactg gcgacgcaag ctttccgcaa
     4681 cgcttgagtc gatgtttgcc gatgatggcg tgaacaagtt gctgaaggat ttggacagac
     4741 ggcgcagagc tgcagcgaag aagaagtaga gtgcggttga tgtcggatgc gatgcatccg
     4801 gcagtcagtg ttatcaaatc accatattca gcagcagaca gcctaccaga ccgcacaccg
     4861 agataatggt ttccagcatc gaccaggatt tgatggtctc gccgatagtc aggttaaagt
     4921 actctttgaa cagccagaag cccggatcgt tcacatgaga gaaaatcaca ctaccggaac
     4981 ccaccgcaat aaccatcagc tctgggctaa cacccgtcgt tgcaatcagc ggtgccgcga
     5041 taccacccgc agtgattgcc gcaacggttg cggaacccag cgcgatacgc agtacggcag
     5101 caatcgacca ggccatcagc agcggagaaa tgttggtttc gtgcatcatg gaagcaatgt
     5161 atttgtccac gccgctgtct accagcacct gcttgaacgc accgccacca ccgatgatca
     5221 acagcatcat cgcaatgatt ttgatggaag aaaccagcgt gtcgttaatc tgatccattg
     5281 aacgaccacg gttcagacca aaggtgaaca tcgcaatcag cacggcaatc agcgttgcca
     5341 ttaccgggtc accgaggaac tccgctaccg gcaggaaagc gtgacctttc ggcaggatca
     5401 tttcggcaat cgcacgcatc gccatcagca caaccggcac cagagaggtc cagacgctga
     5461 cgccaaagct cggcatctct tcttcgctga aggttttcgc gctgtagaga ccttccggga
     5521 ttggcttatc gatacctttc aacacgcgag cgtaaaccgg accggcgaga atcacggtcg
     5581 ggattgccag aatagtaccg tacagcaggg ttttacccat atcggcattg aaaatggtgg
     5641 caatcgcagt cgggcccgga tgcggtggca ggaagccgtg ggtcacagac agtgcagccg
     5701 ccattggtac accaacatac agcaatggaa tattcgcgga agccgcgatg gtaaacacca
     5761 gcggcagcat cagcacgaag cccacttcat agaacagggc aaaaccaacg gtaaaaccgg
     5821 ttaacaccac cgcccactgg atgtgctttt taccaaattt ggcaatcagc gtggtggcga
     5881 tacgttgtgc gccaccgcag tctgccagca ttttgcccag cattgcgcca aaacccatga
     5941 tcagggcaag gctaccgagc gtcccgccga caccggcttt gatggagcca ataactttat
     6001 ccagcggcat tccttgcatt aatccaacag caagcgccac gaggacgaga gcgatgaagc
     6061 cgttcatttt gaagcggatc atcaggagca acaacaagat tacaccgata gcaacaatga
     6121 ctaatggcat gatttacctg gcctttcatt tgttatgggt aacgtcaatt ttctgacgac
     6181 aaactctaat tatcccaatc gggaacagag atattgcggc accacgactg atacccacta
     6241 aaactaatta ttgtagtcag atgtcaggag tatgtttggt acccatgtga atgatacggg
     6301 taacatttgg cgtttgagaa tcaccagagc ggggtaaatt taaattatga gaggttggtc
     6361 atattatcgc ggggaaacga agggagggat ttgccaaagc aatgctgtgc caacgtctgg
     6421 cacatgttca acgtaggccc gaaatgacgc tttagcgtcg catcgggcaa tctacaaaag
     6481 aggggataac ttagtagtag gagtgttcgc cgcgctggtg ttcggtgaga tcgcgcacac
     6541 ctttcagctc cgggaattcg ttcagcagct gcttctcgat cccttctttc agcgtcacat
     6601 cgaccatgga acaaccgtta cagccgccgc caaattgcag aatggcgtaa ccgtcttcgg
     6661 tgatttccat cagcgaaacg cgaccaccgt gaccagcaag ctgtgggttg atctgcgact
     6721 gcagcatata ctccacgcgc tccatcagcg gtgcatcgtc tgccacttta cgcattttgg
     6781 cgttcggggc tttcagcgtt aactgagaac ccaactggtc ggtaacaaag tcgatctctg
     6841 catcttccag gtatggtgcg cttaactcat caacatacgc ggtcagcagg tcaaatttca
     6901 gggcagtgtc ggtggcttcc acggcatccg gcggacaata agaaacacca cattcagcgt
     6961 taggcgtgcc agggttaatc acaaatacgc ggatttgtgt cccttcttcc tgatttgcca
     7021 gcagtttggc aaagtgcgct tgtgcagcat cggaaatacg gatcatagta atggcctaat
     7081 agttgactat tttagttggt tataatacgc ccatcatcga ggctctacaa ggttcgacaa
     7141 aggcaccaga cctggacagt cgccgcacca ttgcgtaaaa gcaactgcgc aatctctgcg
     7201 acggtacttc cggtggtaac gacatcatcc acaatcacca tatggcgacc ttgcacgggc
     7261 aattcaagac gaaaggcatt tttcaggttg cgcttgcgca gccgggcact gagaaaatgc
     7321 tgggtcgcag tggcccgtgt acgtgtgacg gcttcgctat cccattggca gtgcaaccag
     7381 cgtgataacg gctgacacag caaatcgctc tgattaaatc cccgacgcca gtgacgccgc
     7441 tgccataacg gaacgctgat gatgcgatcc ggcaattgca acccggtggt gcgacgagcg
     7501 tgtaagactt ccaatagtaa cagacgtgac agggcgctgg cgatttcact gcgccgggaa
     7561 aatttaagct ggtggataag cggacttaac ggcggcgcat agtcggcaac cgtgaccagt
     7621 ctttgccagg gcggcggttt ttgcaggcag cgaccgcagg gaagatggga gtgtgtggcg
     7681 ggtaatccac attgtgggca taacgtttta tctgtgcggg tggcgcgtga acagaccgaa
     7741 caaatccccc aatgacctaa cgccagtggc attcggcata gccagcataa tcccggtact
     7801 gttagcatat gttcatcctt gtaagtcaaa agagaacaat agcggatgaa taacatctgg
     7861 tggcagacca aaggtcaggg gaatgttcat cttgtgctgc tgcacggatg gggactgaat
     7921 gccgaagtgt ggcgttgcat tgacgaggaa cttagctcgc attttacgct gcaccttgtt
     7981 gacctgcccg gcttcgggcg tagccgggga tttggtgcgc tgtcacttgc tgatatggcc
     8041 gaagccgtgc tgcaacaggc acctgataaa gccatttggt taggctggag tctgggcggg
     8101 ctggtggcaa gccagattgc gttaacccat cccgagcgtg ttcaggcgct ggtcaccgtg
     8161 gcgtcgtcac cttgttttag tgctcgtgac gagtggccgg ggataaaacc ggacgtgctg
     8221 gcgggatttc agcagcaact cagtgatgat tttcagcgta cagtggagcg gttcctggcg
     8281 ttacaaacca tggggactga aacggcgcgc caggatgcgc gggcgttgaa gaaaaccgtt
     8341 ctggcgttac cgatgccgga ggttgacgtg cttaatggcg ggctggaaat cctgaaaacg
     8401 gtcgatctcc gtcagccgct gcaaaacgtg tccatgccgt ttttgcgatt gtatggctat
     8461 ctcgacggtc tggtgccgcg caaagtggtg ccgatgctgg ataaactttg gcctcacagc
     8521 gaatcatata tcttcgccaa agcggcccat gcgccgttta tttcgcatcc ggtcgagttt
     8581 tgtcacctgc tggtggcgtt gaagcagagg gtgttggtgg taagtgagag ctaattattg
     8641 tgggtcgatt aaattcacca gtttactgat aacttcacta atcaattttt catcggcaac
     8701 ggtatgaaat ttagcgccac gggcaataag atcgacggct tttagctggt gacaaagtac
     8761 aacgccatgc aggttaccgg tttgcgtatc acgggggagg acgttcaccg tcacccctgt
     8821 ggaacgtgct gcatttgccc ctgttgaaat cgggcagcac atagcaactt ttaaaacatt
     8881 gttgagtttt ttgtccgtta ccacaatgca ataatgtggc ccctgaagtt catgcccggc
     8941 aaccggatca gggttaaaat accagatctc accacgatgt ggcgtccgtg cctttaccat
     9001 gcttcattac cttttttccc ttcagcccat ccttctgtgg actgatttag ctccgtaatt
     9061 tcgctttcat cgatttggct caacaactgc gcgacggtaa gacgcccacg cggtttgtgc
     9121 tctgtggcgc gcaaattaac gctatcgcct tttttctcca cggtaagctc tgtgcccggt
     9181 gaccagccaa gatcgatggc aagatctcgt gggatagtaa cgatcatggc tcctccttgt
     9241 tgccgtaatt tcactgtctg catatcagtt ccctaagtgc tactttgtgt tacatgcaag
     9301 atagcacaaa gtagcactta agcagggtag agtatctact gac
//
LOCUS       DAZPEU010000153         9236 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPEU010000153 DAZPEU010000000
VERSION     DAZPEU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.154"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(93..2054)
                     /gene="topB"
                     /locus_tag="ACHU2L_003177"
     CDS             complement(93..2054)
                     /gene="topB"
                     /locus_tag="ACHU2L_003177"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR1357923.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2059..3102)
                     /gene="selD"
                     /locus_tag="ACHU2L_003178"
     CDS             complement(2059..3102)
                     /gene="selD"
                     /locus_tag="ACHU2L_003178"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR1357924.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3219..3770)
                     /locus_tag="ACHU2L_003179"
     CDS             complement(3219..3770)
                     /locus_tag="ACHU2L_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR1357925.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3931..5787
                     /gene="sppA"
                     /locus_tag="ACHU2L_003180"
     CDS             3931..5787
                     /gene="sppA"
                     /locus_tag="ACHU2L_003180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR1357926.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5954..6970
                     /gene="ansA"
                     /locus_tag="ACHU2L_003181"
     CDS             5954..6970
                     /gene="ansA"
                     /locus_tag="ACHU2L_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR1357927.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            6981..7622
                     /gene="pncA"
                     /locus_tag="ACHU2L_003182"
     CDS             6981..7622
                     /gene="pncA"
                     /locus_tag="ACHU2L_003182"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR1357928.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7715..9073)
                     /locus_tag="ACHU2L_003183"
     CDS             complement(7715..9073)
                     /locus_tag="ACHU2L_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1357929.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
BASE COUNT         2273 a         2472 c         2281 g         2210 t
ORIGIN      
        1 aattttatcc atgcaaaaaa atatccaacg aactttcttg atccgctgga tatttcagaa
       61 tatgactcga atagcacgaa agattcgctc gcttacgcta tcgccccgct tccggcttca
      121 tctgctggcg gacttttttt cgcactacgt ttacgcggtg cagccttttt cttatcagca
      181 ctgccaccac tgcccggagc cacaatgccg cgaaactgcc gcaccggcgt acgtttggct
      241 tgatcaataa gctgatatag cgtccccacc agcggctgca taaagtcctg atagcgacac
      301 tgcttttcgc tgatttgcgt cagcaccgat tcccagtgcg cggtcatgtc cggtcgcgtc
      361 gccatttctg gcagcgaatg gaataacgct tttccggcgt cggtggagtg gatatagcgc
      421 ccttttttgg tcagaaaacc acgcttgaac agcagttcaa taatcccggc acgggttgct
      481 tctgtcccca gaccatcggt cgcacgaagg acctttttca gatctttgtc ctgcacaaag
      541 cgcgcgatcc cggtcatcgc cgaaagcagt gttgcatcgg taaaatggcg cggcggctgg
      601 gtttgccgct ctaccacttc acctttttca cacagcaact catcgccttt cgccaccaca
      661 ggcaatggcg tgccgtcgtt ttcttcatcg cgctctttgc tgcctaacag cgtgcgccag
      721 cctgcttcag caagaaaacg ggctttagcg acaaatttgc ctttggcaat gtccagttcg
      781 ataacacact tgcggaacac cgcatccggg cagaactgca tcaggtattg acgggcaatc
      841 aggttataga ccttcgcttc gttctccgtc aggttgatcg cagaactccg tgcggtcggg
      901 atgatggcgt ggtgcgcatc gacctttttg tcatcccaac agcggttgcg tatatctgga
      961 tctaccactg gctgcggcaa cagatccggt gcatgaacgc tgatggcatt catcaccgcg
     1021 tggcgtccgg caaaatgttc ttctggcaaa tagcgacaat cggaacgcgg ataagtgatt
     1081 agcttgtgcg tttcgtacag cttctggcag atatcaagca cgttctgcgc gctcagaccg
     1141 aagcgttttg ccgcttcaat ctgcaacgcc gaaagcgaaa acggcagcgg cgcggattct
     1201 gattcccgtt tatcgttata gctggtgaca atagccggtt gaccactaat gcggttaacc
     1261 acatgctccg ccagtggacg atgtaacaag cgcccttctt catcctggta cggttcacac
     1321 gcttcgctcg gttgccagat agcggtaaac cgctcatcgg caggtgtcac gatatgtgct
     1381 ttgacttcaa agaagtcttt cgccacgaag ttttcaatct cttcatcgcg gcgcaccacc
     1441 agcccgagca cgggtgtctg cacgcgtccc acggaaagta cgccctgata accggcattg
     1501 cgaccgagaa tggtatacgc acgggtcata ttgatgccgt acagccagtc ggcacgcgct
     1561 cgcgccagcg cagaaacgca caatggcaca aactcactgt tggaacgaag acggtcgatc
     1621 gcccgctcaa ccgcctgcgg gttcaggtcg tttatcaagc aacgctgcac ctgctggcgc
     1681 ttttccggtg ccagttgcag atagtccagc acttcatcca ccagcaattg cccttcacga
     1741 tccgggtccc cggcgtgaac gatttcgctg gcttcatgca ggaagcgttt gatgacgtta
     1801 agttgtttgg tcacggaggg tcgtggctgt aattgccact tttccgggac aatcggcaaa
     1861 tccgcaagat tccagcgcgc atagcggctg tcgtaggcgt ctggctgcgc ctgctcaagc
     1921 aggtgaccga tacaccaagt caccacctga ccattaccgc actcgataaa gccatcgcct
     1981 ttccggtgcg gtttgggcag gacatcagca atggcgcgcg ccagactcgg tttttcggca
     2041 ataaacaacc gcattaaatt aacggatctc aaccatggca cgaccgccgc gcgctggcac
     2101 cagttcgcca atcgccgtca gttcaatgcc aaactcggcg gctgtagttt taacctcatt
     2161 ttctgcttcc ggcatgaccg ccagcagcaa accgccagaa gtttgcggat cgcacagcag
     2221 atcgcgcact tcacgcggca tttcacccat cagatgaccg tagctggcaa agttacgttc
     2281 agtgccgcca ggtactgcgc ccaacttaat gtactcttca acaccgggga gtttcgggat
     2341 cgcttcatag tcgacgcgtg cctgcacacc agccccctga cacatttcgc tcaagtggcc
     2401 cagcagacca aagcccgtaa cgtcggtcat cgcttttacg ccttcgatgt tggcaaagga
     2461 cgcgcctgcg atgttcatcc ggcacatcac ttccgtcgcc agtccctgat gttctggttt
     2521 caacagtgat tttttctcag ccgtggtaag aacgccgatc cccagcggtt tcgtcaggaa
     2581 cagtttgcat ccggcttgtg cggtactgtt tttcttcacc cgctcggtcg gtacgatccc
     2641 cgttaccgcc agaccaaaaa tcggctccgg cgcatcgatg gagtgaccgc cagccagcgc
     2701 aatacccgcc tgacggcatg catagcgtcc accttcggtt acttcgcggg caatttccgg
     2761 ggaaagtttg ttaatcggcc agccgaggat cgcaatcgcc ataatcggtt tgccccccat
     2821 cgcgaagata tcgctgatgg cgttagtcgc cgcaatgcgg ccaaaatcga aaggattatc
     2881 aacgatcggc ataaagaagt cggtggtact gataacgctg gtgccattgc ccagatcgta
     2941 caccgccgca tcgtcgcggg tttcattacc cacaagcaaa ttcggatcaa caaacttcgc
     3001 ctgctcactg tgcaggatgg tttccaacac ttttggggaa attttacagc cgcaaccagc
     3061 tccgtggctg tattgggtca aacgaatcga gttctcgctc atggacatct cctgtcaatg
     3121 caatccgggt atggtaaccc tcattccgtg aagtgataag tgagagtgtc tgaattcctg
     3181 cgcctttgct cacaatccag acagtttcgc gacaattatc agaaataagt tacaaacggc
     3241 gtcgggtccg ggacgttaat cgacgtagat gctttcaact gcggcgtgcc gaggtagaga
     3301 aaaccgacaa ttttatcctg ctcacggcaa ccgaatgctt cacgcactac cggactttca
     3361 gttaatgcgc cactgcgcca gatgccgcca aatccctggg caacggctgc catttgcatc
     3421 gccatgaccg cgcatccggc agacatttcc tgttcccatc gcgggacttt atgattttct
     3481 ttgcatttcg ccaccaccgt gatgatgagc ggtgcgcgga acggcgcatt acgggctttg
     3541 tcgatagctt tatcatcact accggcagca atcgccccct gttccagtac ggcgctgaaa
     3601 cgctcgcacc cttccccttc aatcacaaaa aaatgccacg gttgcatgga cttatggtcc
     3661 ggcgcacgca tacccgcacg caggatgttt tgcagttgtt cacccgttgg cgcgggttca
     3721 gccaggcggg aggcgctacg gcgattgatc aatagttcga gtgcatccat ttgattaact
     3781 cctgtcgtga tatttattca caaaattaac acgagagtgg attttgttac agcacagtcc
     3841 gcaattcctg ctgacaagtg ccggttgggt cattacgata accacatcta ttgcgcctgt
     3901 gacaggtgtg accttaagtt gggagaatac atgcgaaccc tttggcgatt tattgccgga
     3961 ttttttaaat ggacgtggcg tctgctgaat ttcgtccgtg aaatggtact taacctgttc
     4021 tttattttcc tcgtactggt tggtgtgggg atctggatgc aggtcagtgg tggtgattcg
     4081 aaagaaacgg ccagtcgtgg cgcactgctg ctggacattt ctggtgtgat cgtcgataaa
     4141 ccagacagtt ctcagcggtt tagtaaatta agccgccagc tgcttggtgc cagttccgat
     4201 cgtctgcagg aaaactcact gtttgatatc gtcaacacca ttcgccaggc gaaggacgac
     4261 cgcaatatca ccggtatcgt gatggatctg aaaaacttcg caggcggcga ccagccgtct
     4321 atgcagtaca tcggcaaagc cctgaaagag tttcgtgaca gcgggaaacc ggtttatgcc
     4381 gttggcgaga actacagcca ggggcaatat tatctcgcca gtttcgccaa taaaatttgg
     4441 ctgtcaccgc aaggcgtggt tgatctgcac ggttttgcta ccaacggtct gtactacaaa
     4501 tcgttgctgg ataagctgaa agtttccacc catgtgttcc gcgtgggtac gtataaatct
     4561 gccgttgaac cgtttattcg tgatgatatg tcaccggcag cccgcgaagc tgacagccgc
     4621 tggattggtg agctgtggca aaactatctg aatactgttg ccgctaaccg gcagatccct
     4681 gctcagcagg tattccctgg cgcgcaaggg ttgcttgagg gtttaaccaa aaccggtggc
     4741 gataccgcga aatatgcact ggaaaacaag ctggtcgatg cactggcatc gagtgccgaa
     4801 atcgaaaaag ctctgaccaa agagttcggc tggagtaaga ctgataaaaa ttatcgcgcc
     4861 atcagttatt acgattacgc attgaaaacg ccggcagata ccggtgacag catcggtgtc
     4921 gtcttcgcta atggcgcaat tatggatggc gaggaaactc aggggaatgt tggcggtgat
     4981 accactgcgg cacaaatccg cgacgctcgc cttgacccga aagtgaaagc gattgtcctg
     5041 cgtgttaata gcccaggcgg cagcgttacc gcgtctgaag tgattcgcgc tgaactggca
     5101 gcagcccggg cagcgggtaa gtctgtggtt gtatcgatgg gcggcatggc ggcatctggt
     5161 ggttactgga tttccacgcc agctaattac attgtggcta accccagcac cctgaccggt
     5221 tctatcggta tcttcggcgt gatcaccacc gtagaaaata gtctggattc gattggtgtt
     5281 catactgatg gtgtctcaac ttcaccgctg gcggatgttt ctatcaccag ggcactgccg
     5341 ccggaagcgc agcagatgat gcaattaagc attgagaatg gctataaacg ctttatcacg
     5401 ctggttgctg atgcgcgtca ttcgacgccg gagcaaattg ataaaatcgc ccagggccac
     5461 gtctggaccg gtcaggatgc aaaagctaac gggctggtcg atagtctcgg ggatttcgat
     5521 gatgcggttg ccaaagcagc agagctcgca aaagtgaaac agtggcatct ggaatactac
     5581 gttgatgaac cgaccttctt cgacaaagtg atggacaaca tgtctggttc tgtccgggca
     5641 atgttgccag atgcgttcca ggccatgtta cctgcaccgc ttgcctcggt agcctctacc
     5701 gttaaaagtg aaagcgacaa gctggccgcg tttaacgacc cacaaaaccg ttatgcgttt
     5761 tgcctgacct gcgccaacgt gcgttaagtc ttgtactgag tggccgacag atcgtcggcc
     5821 acattatttt ttacctcgac gaatcctctt cccgctgtct cgccccatat ttccttatcc
     5881 gcatagtatc aggtgcgctc ctcctgcctc acgtatatac ttttgctctt tcgatatcat
     5941 tcatatcaat atcatgcaaa agaaatcaat ttacgtcgcc tacacgggcg ggaccatcgg
     6001 gatgcagcgt tccgagcagg gttatattcc ggtgtcaggt catctacaac gccaactggc
     6061 gctgatgccg gaattccatc gcccggagat gccagatttc accattcatg aatatacgcc
     6121 gttgatggat tcttctgata tgacgccgga agactggcag catattgctg aagatattaa
     6181 agcgcactat gacgactatg atggttttgt cattctacac ggtaccgaca cgatggcgta
     6241 taccgcctct gcgctgtcgt tcatgctcga gaatctcggt aaaccggtca ttgtgacagg
     6301 gtcacaaatc ccgctggctg agttacgctc tgacggacaa attaatctgc tgaatgcgtt
     6361 gtacgttgcg ccgaattatc cgatcaacga agtaacgctc tttttcaata accgattgta
     6421 tcgcggcaac cgcactacca aagcccatgc cgatggtttt gatgcgtttg cctctccaaa
     6481 ccttcctccg ttactggaag ctggtatcca tattcgtcgt ttgaatacgc cacccgcccc
     6541 gcacggtgaa ggggaattaa tcgttcatcc aatcacgcca caaccaattg gcgtggtgac
     6601 gatttatcca gggatttctg ctgacgtcgt gcgcaatttt ctgcgccaac cggtgaaagc
     6661 attgattctg cgctcctatg gcgtgggtaa tgcgccacaa aacaaagcct tcctgcagga
     6721 attacaagaa gccagcgatc gcggtattgt ggtggtcaac ctgacacaat gtatgtccgg
     6781 taaagtgaat atgagtggtt atgccaccgg taacgccctc gcccatgccg gcgttattga
     6841 cggtgcagat atgactgtag aagccacgct aaccaaactg cattacctgc tgagccagga
     6901 actggatact gaaaccattc gcaaggccat gagccaaaac ctgcgcggcg aactgacgcc
     6961 ggatgattaa ggagactgca atgccccatc gcgccctgtt actggtcgat ttacaaaatg
     7021 atttctgtgc tggtggcgcg ctcgccgtac cggaaggtga cagtacggtg gaggttgcta
     7081 accgccttat tgactggtgc cagtcgcgcg gagaagcggt tatcgccagc caggactggc
     7141 atccggcgaa tcacggcagc tttgccagcc agcacggcgt agagccttat acgccaggcc
     7201 aactcggcgg cctgcggcaa actttctggc ctgaacactg tgtgcagaac agtgaaggcg
     7261 cacaattaca tccgttactg aatcaaaaag agatcgcagc ggtgttccat aaaggcgaaa
     7321 atcctttagt tgacagttac agtgcctttt ttgataacgg acgtcggcag aaaaccgcgc
     7381 ttgatgactg gttgcgcgat catgaaatcg atgaattgat cgttatgggc ctggctactg
     7441 actattgcgt gaagtttacc gtgctggacg cgttacagtt aggttataag gtaaacgtga
     7501 ttaccgatgg ctgtcgtggc gtgaatatcc agccccagga cagtgcacac gcgtttatgg
     7561 agatgtcagc agctggggca acgctatata cgctggcaga ctgggaagag acgcaggggt
     7621 aattttacgc tggcctacaa ttctgtactg gcattgtagg ccaaataaaa cacgtcagtg
     7681 gtacatctgg caattgatgc catcaacgaa agatttaatt cacctcagaa atctcttcca
     7741 gcgacacctt ccgcgtttcg atgccaaaaa tggagagaac cagcgcacag agcactaaca
     7801 tgacaccaag caccataaac accgtgatcg acccataatg tgttaataat gccgcaacgc
     7861 catagggcgt gaaaactgcg acgatccgtc cgacggcatt aacgaaaccc gaaccgcgca
     7921 ggcgtaaatg cgttggccaa agctccggga tataaaccgc cgacgcgaag caaacataca
     7981 tgtataaaaa gaagatcatc accagaccat agattaaaat cgcccactct gtagtctgac
     8041 ttgaatagat atagcctaac acggcaataa taataagtaa ggcggagcca aataatcgac
     8101 gaggaaaatg atcaataatt aatgccgcaa taaatattcc taccggagca ccaatcataa
     8161 taacagcggt cattaatatt gatttatcga catcaatgcc ggagttaaca aatatggtcg
     8221 gtatccatac ggtaatggta taaagcgaaa tgttcattgc aattaaaaca gttatcgcaa
     8281 ctaatgtacg tcgtaacatt tcccctttaa acaggagcct gaaggtaccc ttgattacct
     8341 taaccttgct gttgctctgg ttcaaagtaa gctggggtaa acgaatactc ttctcccttt
     8401 caatttgctg ctctacttca cgaagttggc tttctgcacc ggcgatttgc cctttccctt
     8461 ccagccatcg tggcgactcg ataaagtatt tacctgagag aaaccaggct aacagtatgc
     8521 caataccacc cagaagaaac attattcgcc aactaaaaaa agcgataacc accacgccta
     8581 tcgccgcaga cagcatgggc gaccagttac caacaaatga gagccgcgcg gaccattttc
     8641 cacgcaccgt cgcggggata aactccgtaa atgaggcata gccaaccata atcagcgccc
     8701 ccattcctgt tcccatcagg aagcgaaaga aaatgagcca gtacatatca gggacaaaag
     8761 cggcccctgt tgcagcaata ccgacgatga gaagatttat gcgaaacgcc ctgcgccgcc
     8821 caaagtagtc accaataaac ccaccagtaa gtgagccgat gaaataacca aacattaatg
     8881 ccgaggtaaa ggcggcatag aggaaattat ttgaccatcc attgcttacc agctttgcta
     8941 agacgacatt acctgagtaa ctcaaaaacc ccgttaataa cagactaaag cttattatac
     9001 caaatatacg ataatgaaac cgggccaaag gcaagcggtc cagtcttgcg ccaatttgat
     9061 catattgttc cattgggtgc ctcggatttg ctataaaagg tagcaattgc ggcaaattca
     9121 cttgccgcat ctttatcatc attttatttc cagtcgcagg aacaaataat attgacaacg
     9181 tgtgcttaaa ttaccacacc agctggatat tattgtcttt gcaaaatgct atccac
//
LOCUS       DAZPEU010000154         9162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPEU010000154 DAZPEU010000000
VERSION     DAZPEU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.155"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            92..694
                     /gene="ais"
                     /locus_tag="ACHU2L_003184"
     CDS             92..694
                     /gene="ais"
                     /locus_tag="ACHU2L_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR1357930.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(733..1158)
                     /gene="nudI"
                     /locus_tag="ACHU2L_003185"
     CDS             complement(733..1158)
                     /gene="nudI"
                     /locus_tag="ACHU2L_003185"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR1357931.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1437..1979
                     /locus_tag="ACHU2L_003186"
     CDS             1437..1979
                     /locus_tag="ACHU2L_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR1357932.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            2066..3268
                     /locus_tag="ACHU2L_003187"
     CDS             2066..3268
                     /locus_tag="ACHU2L_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR1357933.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3332..3793
                     /locus_tag="ACHU2L_003188"
                     /pseudo
     CDS             3332..3793
                     /locus_tag="ACHU2L_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3834..4784)
                     /locus_tag="ACHU2L_003189"
     CDS             complement(3834..4784)
                     /locus_tag="ACHU2L_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1357934.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4977..6167)
                     /gene="glpC"
                     /locus_tag="ACHU2L_003190"
     CDS             complement(4977..6167)
                     /gene="glpC"
                     /locus_tag="ACHU2L_003190"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR1357935.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6164..7423)
                     /gene="glpB"
                     /locus_tag="ACHU2L_003191"
     CDS             complement(6164..7423)
                     /gene="glpB"
                     /locus_tag="ACHU2L_003191"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR1357936.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7413..9041)
                     /gene="glpA"
                     /locus_tag="ACHU2L_003192"
     CDS             complement(7413..9041)
                     /gene="glpA"
                     /locus_tag="ACHU2L_003192"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR1357937.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
BASE COUNT         2121 a         2327 c         2438 g         2276 t
ORIGIN      
        1 ttaatattac accaactgct aattatcgcc agaaatgtcg ctcaatttca cggtaattgt
       61 ctggttgcgc ttgtctatag gtggagttta cgtgttagct ttttgccgct catcattgaa
      121 gtcaaaaaaa tatttcatca ttttactggc gctcgctgca attgccggac tgggtactca
      181 tgccgcctgg agtagcaatg gtttgccacg tatcgacaat aaaacattgg ccagactggc
      241 acagcagcac ccggttgtcg ttttgtttcg ccatgctgaa cgttgcgatc gttcaaccaa
      301 tcaatgcttg tcagataaaa caggtattac ggttaaaggt acccaggatg cccgtgaact
      361 gggcaacgct tttagcgctg acatctctga tttcgatctt tattccagta ataccgtccg
      421 gaccattcag tcggcgacct ggttttccgc gggtaaaaag ctgacggtag ataaacgact
      481 tcttcagtgc ggtaatgaga tttatagtgc aattaaggac ttacaaagca aagcgcctga
      541 taaaaatatc gttattttca cccataatca ttgcctgaca tatattgcta aaaataagcg
      601 tgatgcgata tttaaacctg attatctgga tggtttagtc atgcatgtgg aaaaaggcaa
      661 agtttatctg gatggggaat tcgttaacca ctaaattttc aatctgacag ccagtgatgg
      721 ctgtcatcat tgttaaagaa gacctttcaa acgtaacgtt tttcgggtgg caacatttaa
      781 atcataatgc accagatctt caggttttac ccacgcgtag tcctgaaact cttcgtttat
      841 tttcacttct cggttggcag aaacgcagtc aaaaatcagg taaatcatat aaatctcttc
      901 cttgcgacca tctgcatatg tcttggtgcg aatatcatcg ctgaaggtcc acggcgtgat
      961 ttctgtcaaa agcagctgtt ctcccagttc ttcgcgaatt tcgcggcgta gtgcctcttc
     1021 aattcgttcg ccagactcca cgccgccacc cgaaagcgcc cattgaccgg ggaaaacgcc
     1081 gcgatcgtcg gccattttgc acagcaaata agcaccatca ttttgaatca aagggcatac
     1141 aatagtccgt tgtcgcacag gggtattcct tataaataaa aagaaaccat gagaatacgg
     1201 taactcagaa atatgtgaaa gtgacagtaa tctcttttgc aatgataact gcacttatta
     1261 ttatttttgt gtgtatcggt aaagtgtgtt tattttttcg gcgtttaatt atttaagaag
     1321 tatttagtta caataagtga ctagatccct tttttcgctc attgaatcct ctgtgccagg
     1381 cagtacaatc ccgcagcttt gggaattttg attaattgct tgtgggaaag gggataatga
     1441 aaaaaattgc gctagcaggt ctggccggaa tgctgttggt ttctgcatcg gtcaatgcaa
     1501 tgagcatcag cggccaggcg ggtaaagaat acaccaatat tggtgtcggt tttgggactg
     1561 aatcgacggg cctggcttta agcggtaact ggacacataa cgacgacgac ggtgacgtcg
     1621 cgggcgtggg gctggggttg aatctgcctc tcgggccgtt aatggcgacc gttggcggaa
     1681 aaggcgtgta caccaacccg aattacggcg atgaaggtta tgccgcagcg gtaggaggtg
     1741 gtttgcagtg gaaaattggc aacagcttcc gtttgtttgg cgagtattac tactctccgg
     1801 attcgctctc cagcggtatt caaagttatg aggaagcgaa tgctggcgcg cgttacacca
     1861 ttatgcgtcc agtcagtatt gaggcgggtt atcgctacct gaatctgtcg ggtaaagacg
     1921 gtaaccgcga caacgctgtg gctgacggcc tgtatgttgg ggttaacgcc agtttctgat
     1981 cccttccggc gcggcattca gtttcttact acgccttatc tccctttgcg ttatagtgat
     2041 tcgataacaa aagcaggaga taaccatgtt aaaagtggaa atgctatcca ccggggatga
     2101 agtgttatac gggcaaatcg ttgacactaa cgctgcctgg ctggccgatt ttttctttca
     2161 tcaggggctg ccattatctc gccgcaatac ggtgggggat aaccttgatg acttagtcac
     2221 cattcttcgc gaacgtagtc agcacgccga tgtgctgatc gttaacggcg ggctgggacc
     2281 gaccagcgat gatttaagcg cactcgccgc tgcgacagca aaaggtgaag gcctggtgct
     2341 gcatgaagcc tggctcaaag agatggaacg ctatttccac gaacgtggac gagtaatggc
     2401 accgagcaac cgtaaacaag cggagctgcc tgccagtgct gaatttatca ataacccggt
     2461 aggcaccgcc tgtggttttg ccgtgcagct taatcgttgc ctgatgttct ttactcccgg
     2521 cgtaccgtca gaatttaagg tgatggtcga gcacgaaatc ctgccgcgcc tgcgcgagcg
     2581 tttttcttta ccacagccgc cggtttgtct gcgtttaact acctttggtc gttcggaaag
     2641 cgatctggca caaagcctgg acactctaca actgccgccg ggcgtaacaa tgggctatcg
     2701 ctcctcaatg ccgatcatcg aactgaaact caccggaccg gcaagcgagc aacaggcgat
     2761 ggaaaaactg tggctggacg ttaaacgtgt tgccggacag agcgtgattt tcgaaggcac
     2821 cgaaggactg cccgcgcaga tcagtcgcga attgcaaaac cgccagttca gcctgacgtt
     2881 gagcgagcaa ttcaccggtg gtttattggc tttgcaactt tctcgcgcag gtgctccatt
     2941 gctggcgtgt gaagtgattc cttcacagga agaaaccctg gcgcaaactg cgcactggat
     3001 tacagaacgg cgggccaacc attttgccgg gctggcactg gctgtttcgg gtttcgagaa
     3061 cgagcatctc aactttgcgc tagccacgcc agacggcact ttcgctctgc gtgtgcgttt
     3121 cagcactaca cgctacagcc tggctatccg tcaggaagtg tgcgcaatga tggcactgaa
     3181 tatgctacgc cgttggttaa acggccagga catcgctagt gagcatggct ggattgaggt
     3241 tgttgagtcc atgactttat ccgtctgatt tcgccactct gtgccggttt actgcaccgg
     3301 cacattcccc ctccggcaaa atttgtccaa ccctccctac ggtgagcgtg gtgttggggc
     3361 cagtgtggca cgggctgcgc gctggggacg cattgagaat tacatggcgc aagtcaacga
     3421 ttcgctttgt ctgttagtgc aggtggaaag taaaacggca ctggataacc tggacgagat
     3481 cctcgacgtc gaagggattg atggcgtgtt tattggacca gcggatcttt ctgcgtcgtt
     3541 gggctacccg gataacgccg ggcacccgga agtgcagcga attattgaaa ccagcattcg
     3601 gcggatccgt gctgcgggta aagcggctgg ttttctggct gtggctcctg atatggcgca
     3661 gcaatgcctg gcgtggggag cgaactttgt cgctgttggc gttgacacga tgctctacag
     3721 cgatgccctg gatcaacgac tggcgatgtt taaatcaggc aaaaatgggc cacgcataaa
     3781 aggtagttat tgatatcaaa ggcccatggg gatcggctgt gggcctgtgt taattagtgg
     3841 ttattcgctg ccagatcggc ttcgcttagc tgggtggccg cgagcacttg gtcacgatca
     3901 atgccctgtt ccagcatgcg caaagcaatt ttaatggctt gttcatgcat accttcctgc
     3961 aaaccttcta atttaccttg ctgcaaacct actaatttac cttcttgcca gccaatttga
     4021 tgtccttcct gccgtagccg ttcagcaata gtcattaatc tctccttttg tagtggtgaa
     4081 cgttcggcaa tctcctgaat aaaacgggtg aaacgggagg tatcgccgca ttgcagcaga
     4141 taattaaaca gtgtttgtag ctggctgtca ttagtgaagc ctctaaccaa aagcgtggtg
     4201 atcctgtcga ccatgccgat taaatcgtgg tcgcgaatat gcttttgaat cagttccagc
     4261 agagctatac gccgatgttg catgatctca tcgtcaggta cgatggtgat atccaccaac
     4321 ggaaaagctt cggtgtacaa ctgccgggca agttgcggat cgtcaaactc atccagccag
     4381 ttaagtgagt acgggtaggg cgatgtttcg ccatgataaa acagcaatgg caccaccagc
     4441 ggaactctgt catagccttt atctaggtga ctctgcatgg cggcagtggc atagcgcatt
     4501 ggccgaaaag ccatattctt ttctgcagag ctttgatgtt caatcacgca gtagatatag
     4561 ccgtcaccgt cgctggtttc cacggaccac aaaacatccg agtagtaagc gcgtaaactt
     4621 ttttcaataa aactagtggg ttccaggcgt aaggtttgca ggttgcaaag cttgcgcagt
     4681 ggttctggta aatgtatttc gagaaaatcc cgtgcggttt cgggtgtgaa tataaaggtt
     4741 ttaaataccg catcatgcgg ggaggaggtt gttgattcgg tcatggttgt ccgtcacctg
     4801 atatttatga tgacgcgaca ttacggttat cccaggcctt tgacatctgc cagtttcagc
     4861 ctttgcgaga cgttttcaga ggaattttca ctgcgttgca acgcatacat tgggcacgga
     4921 atcgtgtacg caaaaatgcc cggtaaccac atgcttaccg ggcatcagaa gggagtttaa
     4981 gccagcgcct gggccagtag cgtaatcgga tgttcgcagc gaagacttgt ggacatctca
     5041 atctgccatt tacaggtttc gcagtcggtg accaccagat ctgcgccgct ttcttctatc
     5101 tggcggaaca gtggtgcgcc gatggcttgt gaggtggggt agttctcttt tttgaaaccg
     5161 taagtacccg caataccgca gcactgggaa tccagcaccg ttaactcaag ccccgggatg
     5221 ttacgcaaca gctccagggt gtagagcgtc cagcccattt tttccatatg gcacggagtg
     5281 tgataaacca ctttcagcgg cagcggtttc agcggtaacg ttttgccttc gtccagcttg
     5341 cgccacagcc agcgggttgc cagttcgata tgatcgcgca agcctttgtt gtcgacattc
     5401 agcacttccg ggtattcgtc gcgcagggca aatgtacagg ttgaggaggt ggcaatcacc
     5461 ggaatgcctt ttactcccac agcttcgcgg atcgactcta cattcgtaat tgcctgtttg
     5521 cgtgctttat cggtaaagcc gttggcgatt agcggtacgc cgcagcattt ttctttgctg
     5581 agcagttgta caccggtacc cattgcgttg agcactttaa ttaaatcttt acctaactgc
     5641 ggatggttgt agttaacgaa gcagccgtga aagaaagcga cctggtcttt atattgtgct
     5701 tgctgagccg ctatgctgcg ataccagcga cggaacgtgc cgaaggagta tttcggtagc
     5761 gtgcgacgat gatcgatttt taacgccgca tcaagcagct gccgcaccgg tttcagcgag
     5821 gtagcggtgt tgacgattgg tgcgaacggc gtggaaacgc tacccatcag gtcggtatga
     5881 ctcaacacaa aattacgcag cgacgggcgc gtggtgtcat atttcgcccg cgcgcgctgg
     5941 ataatatcgc caatcttcac atcggacggg caggcgactt cacaacgttt gcagttgatg
     6001 caatatttca gcgcctcgtc atacagtgcg ccatctttca aacgcagacg ctcgccatcc
     6061 ggcccggctt gttttggccc tggataaccg ggattcaccc ggctcaccgg gcaggcggtg
     6121 gtgcagacgg tgcacttaat gcagttttcg aagctggtgt cgttcattgt tggcctcctg
     6181 cgcgttgggc aatctgttga gcggcatgta aagcactgac ggcacaaaca ccgccgccgc
     6241 atccctgggc gatgggatca aatccgccca gcaccgaacc gatggcaaac aggttctcaa
     6301 tggtttgccc tgcctgcgac gggcgtagcg tctcatcagt ggttacaccg aactgctgcc
     6361 acggttgcgg cgcaaaaaaa tcgcccttat accattcacc ccgcgtggcg gtttgtagca
     6421 catcaaggcc gagaatcggc tcgcgaatgc cgttacgttc cgctactagt ccgccactaa
     6481 agaaactgcc gctggcgaga accgcgaaac gtggacgtag cggaatatcg gcgtgattgc
     6541 gggtccagat ttcattcact acgccatttt tacaggtcac ttttttcact tcatcgcccg
     6601 gcatccacac tccaccttgg cgcacaaact ggcgctgtaa ctggttttgc agacgaatgc
     6661 ccagcacgga aggcggcagc gttggcaaaa gcatcagtga acaaggtagt ttttcattca
     6721 accaacgcca cagtttgtca tcggccaggc cgaagcaggc gggcatcagg atcatttcgc
     6781 aggtattggc gacaggaata agcgcatcaa gtaacagcgg ccagttttct tcattatcaa
     6841 ggaaacgggc gatattcacc gcgcgaaatt cggtggcgtt attgcgcagg acatccagtt
     6901 ccggcagctc tatttctgcg gtttcaaccg aaagatccag ttcgcgcaac gaagccgccg
     6961 caagatgcgc ctggaaatcc atcagcccgc ttatccccac tacacatatc ttcttcgcgg
     7021 gtagcggcca gacgggaact tctggcgaac ttaaccaggt tgcacgcaaa gtgccgagcg
     7081 gcgtaatccg ctggtgagcc agttctacgc tgccctgcaa ttgcgcaccg ctttcagcga
     7141 ttaatgcctg cgcctggcaa gcgagatcga gcacgcgttg tggcccgaga agagagtaag
     7201 gatgtgcagg tgcctgctga cgcaaagatt ccagtccact gtgaatgtct gccacgggtt
     7261 gaccatctgg cagatggctc agcaaatcca gcgaaccgga tgagaaatgc aacgcgcttt
     7321 gaccacgcgt gacaatggca cagcgcaggc cgtgtttttg cagttgcagg ccacagagta
     7381 atccggcgag gccgccgccc ataatgacag tatcaaagcg catctttctg ctccttctcc
     7441 agaccacaca gtccctgata aacccagcgg gtaaattcgc tttcgcgcag tgcgtctccc
     7501 caggcgatgg gttgcacgcc tttccagcgt tcgttaagga aggtggaaag ttgctcgata
     7561 gattgcgcgg acgtcgtgac gttaaaccgt tgcagcagtc cggcagcgcg gcaggcgcag
     7621 agttcgccct ggcaggtgcc catccccaca cgggtacgac ggcgtaaatc cagcaggcta
     7681 ttaacgttta aattttctac cgcgtactgc acttcacccg cagttaccgc ttcgcactca
     7741 cataccaggc tacggtgtag acggccttcg ctcagccagg caggcgtgcg atcgccatga
     7801 cgataaaccg cagaaccgcg cagcggggca ggcagggaga tgactttacg caaggtaact
     7861 tcagcgggtt cttgtgaacc aggcagtgcc agatcggcag tcgtacaggg gcgcgtgttg
     7921 cccagtttgc ggcataccgc gtcggtagcc cattcagcca tcagccgata ggtcatcagt
     7981 ttgccaccgg tgatggtgat aaatccgtcc agaccatcgc gttcagcatg gtcgagcagc
     8041 acgatgccac ggctgacgtt acgtccgctc gggtcgtcat cgctggcaac cagcgggcgc
     8101 acgccagaat aggcccgcaa aatgcgcgtt ttcgccatca cgggggccag tttttcccct
     8161 tcacgcagca gaatatcaac ctcttctgcc gtcactcgat tatcgtcaat ctcgttgtaa
     8221 tcaatacgta aagaggtggt accaatcagc gaaatggtat cgccaggcac cagaatatcg
     8281 gcgtcggaag gtttacggca gcggttgatc acatgctggt taatgcggtg atccatgatc
     8341 agcagcgatc ctttcgccgg gaacatgcga atgcgcagat cggcatattc ggcaatgtgt
     8401 tgcccccaga tcccagcggc attaaccacg acaggtgcat gaagggcctg agtttcgccg
     8461 gtgagatggt tacgtacacg aacaccgcac accgtcgcgc cttcacgaat cagccccgtg
     8521 acttcatgag cggtaaggat aacggcaccg tgttctttgg catccagcat gtttgctgcg
     8581 gtcagacgaa atggatcaac ggtgccatcc ggaactttca ccgcgccaat cagtgccggg
     8641 ttaacggcag gttcgataat gcgcgcttgc tgcgggtcta tagcttctgc gctgatccct
     8701 gcttcttcgc aggcgcgaat aaaagtggcc tggaatgaga ggtcatcttc cggcagggtg
     8761 ataaacaggc cgttggttgg ttcaacgcag tgacgtgcaa tgcgtttcag gatctggttt
     8821 tcactaatgc attcgcgggc cgattccgca tcggttactg catagcgcgc accgctgtgc
     8881 agcaggccgt ggttacgccc ggtggcaccg gttgcgatgt cgtggcgctc aaccaaaatc
     8941 acgcgcagcc cgcgcagggc acagtcgcgg gcaatcccgg ctcccgttgc gccgccgcca
     9001 atgataatca cgtcacttga ttgcgagtcg cgagttttca ttgttatccc tctgaagttc
     9061 gttttttacc atttagccat agtaaaaaca tgaattgttt gatttcgcgc atattcgctc
     9121 atgattcgaa agtaaaacgt gatttcatgc gtcattttga ac
//
LOCUS       DAZPEU010000155         9153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPEU010000155 DAZPEU010000000
VERSION     DAZPEU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.156"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..923
                     /locus_tag="ACHU2L_003193"
     CDS             <1..923
                     /locus_tag="ACHU2L_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1357938.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHU2L_003194"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHU2L_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR1357939.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHU2L_003195"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHU2L_003195"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR1357940.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSNSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHU2L_003196"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHU2L_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR1357941.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHU2L_003197"
     CDS             4446..4805
                     /locus_tag="ACHU2L_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR1357942.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHU2L_003198"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHU2L_003198"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR1357943.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHU2L_003199"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHU2L_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR1357944.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHU2L_003200"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHU2L_003200"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1357945.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2091 a         2210 c         2473 g         2379 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatttgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atg
//
LOCUS       DAZPEU010000156         9113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPEU010000156 DAZPEU010000000
VERSION     DAZPEU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.157"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHU2L_003201"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHU2L_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR1357946.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHU2L_003202"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHU2L_003202"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR1357947.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHU2L_003203"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHU2L_003203"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR1357948.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHU2L_003204"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHU2L_003204"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1357949.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVQ
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACHU2L_003205"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACHU2L_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR1357950.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACHU2L_003206"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACHU2L_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR1357951.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACHU2L_003207"
     CDS             6064..6606
                     /locus_tag="ACHU2L_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR1357952.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHU2L_003208"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHU2L_003208"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR1357953.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACHU2L_003209"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACHU2L_003209"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR1357954.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACHU2L_003210"
     CDS             8003..8260
                     /locus_tag="ACHU2L_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR1357955.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACHU2L_003211"
     CDS             complement(8236..8790)
                     /locus_tag="ACHU2L_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR1357956.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            complement(8834..>9113)
                     /locus_tag="ACHU2L_003212"
     CDS             complement(8834..>9113)
                     /locus_tag="ACHU2L_003212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357957.1"
                     /translation="RRALAHCREVAYITLSSFRVNPEFQDFFSSLFRPSCEVFHVVVS
                     TEAHYRDPNILHKYFFDLLFCLLFFHPFCCNRTQNGAFLHTKRLAQGQ"
BASE COUNT         2287 a         2211 c         2272 g         2343 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gtgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgctgca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagaga aaaaatcctg aaattcaggg ttgactctga
     9061 aagaggaaag cgtaatatac gccacctcgc gacagtgcgc taaagcgcgt cgc
//
LOCUS       DAZPEU010000157         8777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPEU010000157 DAZPEU010000000
VERSION     DAZPEU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.158"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            468..1589
                     /gene="ompC"
                     /locus_tag="ACHU2L_003213"
     CDS             468..1589
                     /gene="ompC"
                     /locus_tag="ACHU2L_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1357958.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1701..2756
                     /gene="apbE"
                     /locus_tag="ACHU2L_003214"
     CDS             1701..2756
                     /gene="apbE"
                     /locus_tag="ACHU2L_003214"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR1357959.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2830..3894
                     /gene="ada"
                     /locus_tag="ACHU2L_003215"
     CDS             2830..3894
                     /gene="ada"
                     /locus_tag="ACHU2L_003215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR1357960.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3894..4544
                     /gene="alkB"
                     /locus_tag="ACHU2L_003216"
     CDS             3894..4544
                     /gene="alkB"
                     /locus_tag="ACHU2L_003216"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR1357961.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4620..6263
                     /gene="yojI"
                     /locus_tag="ACHU2L_003217"
     CDS             4620..6263
                     /gene="yojI"
                     /locus_tag="ACHU2L_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR1357962.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6481..6630
                     /locus_tag="ACHU2L_003218"
     CDS             6481..6630
                     /locus_tag="ACHU2L_003218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357963.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTY"
     gene            6739..8105
                     /gene="mqo"
                     /locus_tag="ACHU2L_003219"
                     /pseudo
     CDS             6739..8105
                     /gene="mqo"
                     /locus_tag="ACHU2L_003219"
                     /inference="COORDINATES: protein motif:HMM:NF017817.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(8227..8736)
                     /gene="eco"
                     /locus_tag="ACHU2L_003220"
     CDS             complement(8227..8736)
                     /gene="eco"
                     /locus_tag="ACHU2L_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR1357964.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
BASE COUNT         2138 a         2146 c         2364 g         2129 t
ORIGIN      
        1 ctgtggtagc acagaataat gaaaagtgtg taaagaaggg taaaaaaacc gaatgcgagg
       61 catccggttg aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt
      121 taatgatgat agcgggagtt attctagttg cgagtgaagg ttttgttttg acattcagtg
      181 ctgtcaaata cttaagaata agttattgat tttaaccttg aattattatt gcttgatgtt
      241 aggtgcttat ttcgccattc cgcaataatc ttaaaaagtt cccttgtatt tacattttga
      301 aacatctata gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt
      361 attctgcatt tttggggaga atggacttgc cgactgatta atgagggtta atcagtatgc
      421 agtggcataa aaaagcaaat aaaggcatat aacagagggt taataacatg aaagttaaag
      481 tactgtccct cctggtccca gctctgctgg tagcaggcgc agcaaacgct gctgaagttt
      541 acaacaaaga cggcaacaaa ttagatctgt acggtaaagt agacggcctg cactatttct
      601 ctgacgacaa gtctgtagat ggcgaccaga cctacatgcg tcttggcttc aaaggtgaaa
      661 ctcaggttac tgaccagctg accggttacg gccagtggga atatcagatc cagggcaaca
      721 gcgctgaaaa cgaaaacaac tcctggaccc gtgtggcatt cgcaggtctg aaattccagg
      781 atgtaggttc tttcgactac ggtcgtaact acggcgtagt ttacgacgta acttcctgga
      841 ccgacgtact gccagaattc ggtggtgaca cttacggttc tgacaacttc atgcagcagc
      901 gtggtaacgg cttcgcaacc taccgtagca ccgacttctt tggtctggtt gacggtctga
      961 acttcgctgt tcagtatcag ggcaaaaacg gtagcccgga aggtgaaggc atgaccaaca
     1021 acggtcgtga agctctgcgt cagaacggtg acggcgttgg cggttctatt acttatgatt
     1081 acgaaggctt cggtatcggt gctgcagttt ccagctccaa acgtactgat gaccagaact
     1141 tcggtctgaa ccgttatgat gagcgttaca tcggtaatgg cgaccgcgct gaaacctaca
     1201 ccggtggtct gaaatacgac gcgaacaaca tctacctagc tgctcagtac acccagacct
     1261 acaacgcaac tcgcgtaggc aacttgggtt gggcgaacaa agcacagaac ttcgaagctg
     1321 ttgctcagta ccagttcgac ttcggtctgc gtccgtccct ggcttacctg cagtctaaag
     1381 gtaaaaacct gggtgtcatc aatggtcgta actacgacga cgaagatatc ctgaaatatg
     1441 ttgatgttgg tgcgacctac tacttcaaca aaaacatgtc cacctacgtt gactacaaaa
     1501 tcaacctgct ggacgacaac cagttcactc gtgacgctgg catcaacact gataacatcg
     1561 tagctctggg tctggtttac cagttctaat ctcgatggat atcgaacaaa gggcctgcgg
     1621 gccctttttt cattgttttc agcgtacaaa cacagttttt tggtgtactc ttgcgaccgt
     1681 tcgcatgagg ataatcacgt atggaaataa gctttacccg cgtggcactg ctggttgccg
     1741 cgctcttctt tgttggttgc gatcaaaaac cacaacccgc caaaacccac gctactgaag
     1801 ttaccgttct tgaaggcaaa accatgggta ccttctggcg tgccagcatc ccgggcattg
     1861 atgccaaacg cagtgccgaa cttaaagaaa agattcagac ccagctggac gccgacgatc
     1921 agctgctttc gacctataaa aaagattccg cgctgatgcg ctttaacgac tcgcaaagtt
     1981 tgtcgccgtg gccggtaagt gaagcgatgg ccgatatcgt caccacctcg ctgcgcattg
     2041 gggcaaggac cgatggtgcg atggatatta ccgtcgggcc gctggtgaat ctgtggggct
     2101 ttggcccgga acaacagccg gttcaaattc cgagccagga acagatcgat gcgatgaaag
     2161 ccaaaaccgg cttacagcac ctgacggtca tcaatcagtc gcatcagcaa tatctgcaaa
     2221 aagacctgcc ggatttatat gtcgatctct ccaccgtcgg tgaaggttat gcggcggatc
     2281 acctggcacg cttgatggag caggaaggga tttcccgcta tctggtgtcg gtgggcggcg
     2341 cgctgaacag ccgtggtatg aacggtgaag gccagccgtg gcgggtagcg attcaaaaac
     2401 caaccgataa agaaaacgcg gttcaggctg tggtggatat caacggtcat gggattagca
     2461 cctctggcag ctaccgtaac tattacgaac tggacggcaa acgtctttcc catgttatcg
     2521 atccgcaaac cgggcgtccc atcgaacaca atctggtatc cgtgacggtg attgctccga
     2581 cggcgctgga agccgatgcc tgggatactg gcttgatggt actcgggccg gagaaagcca
     2641 aagaggttgt tcgccgggaa gggctggcgg tttatatgat caccaaagaa ggcgatagct
     2701 ttaaaacctg gatgtcacca cagtttaaaa gcttccttgt cagcgaaaaa aattaaagcg
     2761 caagattgtt ggtttttgcg tgatggtgac cgggcagact aaaggctatc cttaaccagg
     2821 gagctgatta tgaaaaacgc cacatgctta actgacgatc aacgctggca atctgtctta
     2881 gcccgcgacc cgaatgccga cggcgaattc gttttcgccg tgcgcaccac gggtatcttt
     2941 tgccgtcctt cttgccgcgc cagacatgcc ttgcgggaaa atgtctcctt ctacgcaaat
     3001 gccagcgagg cactcgccgc cggatttcgc ccctgcaaac gttgtcagcc agacaaagcc
     3061 aatccccggc aacatcggtt ggataaaatc acccacgcgt gtcgactgct ggaacaggaa
     3121 acgcctgtaa cgctggaagc cttagccgac caggtggcga tgagtccatt tcatctacat
     3181 cggttgttta aagctactac cggaatgacg cctaaagcct ggcaacaggc ctggcgcgct
     3241 cgccgtttgc gcgaatcgct ggcgaaaggg gagagcgtga cgacgtctat tcttaacgcc
     3301 ggattccccg acagcagcag ttactaccgc aaagccgatg aaacgctggg catgacggct
     3361 aaacaattcc gtcacggtgg cgaaaatctg gcggtgcgtt acgcgctggc tgattgtgag
     3421 ctgggtcgtt gcctggtggc agaaagcgag cgggggattt gcgcgatatt gctgggcgat
     3481 gatgacgcga cattaatcag cgagttgcag cagatgtttc ccgctgccga caacgcgcct
     3541 gccgatctga cgtttcagca acatgtgcgt gaagtgatcg ccagcctcaa tcaacgcgat
     3601 acgccgctga cgttaccgct ggatattcgc ggcactgctt ttcagcaaca agtctggcag
     3661 gcactgcgca cgataccttg tggtgaaacc gtcagttatc agcaactggc taacgccatc
     3721 ggcaaaccga aagcggtacg ggccgttgcc agcgcctgtg ccgccaacaa gctggctatc
     3781 gtaataccct gtcatcgggt ggtccgtggt gatggcacac tttccggtta ccgctggggc
     3841 gtgtcgcgta aagcgcaact gctgcgccgc gaagctgaaa atgaggagag gtaatgttgg
     3901 atctgtttgc cgatgctgaa ccgtggcaag agccactggc ggctggtgcg gtaattttac
     3961 ggcgttttgc ttttaatgct gcggagcaac taatccgcga tattaatgac gttgccagcc
     4021 agtcgccgtt tcgccagatg gtcgctccag gcggatatac catgtcggtg gcgatgacca
     4081 actgtgggca tctgggctgg acgacccatc ggcaaggtta tctctattcg cccattgatc
     4141 cgcaaacaaa taaaccgtgg cccgctatgc cacaaagctt tcatgattta tgtcaacgtg
     4201 cggctacggc ggcgggctat ccagatttcc agccagatgc ttgtcttatc aaccgctacg
     4261 ctcctggcgc gaaactgtcg ctgcatcagg ataaagacga accggatctg cgcgcgccaa
     4321 ttgtttctgt ttctctgggc ttacccgcga tttttcaatt tggtggcctg aaacgtaatg
     4381 atccgctcaa acgtttgttg ttggaacatg gcgatgtggt ggtatggggc ggtgaatcgc
     4441 ggctgtttta tcacggtatt caaccgttga aagcggggtt tcatccactc accaccgact
     4501 gccgctacaa cctgacattc cgtcaggcag gtaaaaaaga ataaaaataa gaattattat
     4561 tgctgtgcgc gcgaagatgt ttaaactgcg ggctgtaatt cattgtccgg gttttctgca
     4621 tggaacttct tgtacttgtc tggcggcagt atcgctggcc atttatcagt gtgatggcgc
     4681 taagcctcgc cagtgcggca ttaggcattg gcttaattgc ttttatcaat cagcgcctta
     4741 tcgaaacggc ggataccagt ctgctggtgt taccggagtt tctgggatta ttgctgctgt
     4801 tgatggcagt cactctcgga tcgcaactgg cgctcaccac tttggggcat cacttcgttt
     4861 accgactgcg tagtgaattt atcaagcgga ttctggatac ccacgtcgag cgcattgaac
     4921 aactcggtag cgcctcgttg ctggcgggat taaccagcga tgtgcgcaat atcaccattg
     4981 cttttgtgcg tctgccggaa ctggggcagg ggatcattct cactatcggt tcatcggcgt
     5041 atctgtggat gctgtcgggc aaaatgttgc tggtaacggc tatctggatg gcgatcacca
     5101 tctggggcgg ttttgtgctg gtggcgcggg tgtacaaaca tatggcgacc ctacgtgaaa
     5161 ccgaagacaa gctgtacacg gattttcaaa ctgtacttga agggcgcaaa gagctgactc
     5221 tgaaccggga acgcgccgag tatgtgttta acaacctcta catccccgat gcgcaagagt
     5281 atcgccatca tattattcgc gcagacacct tccatcttag tgccgtgaac tggtcaaaca
     5341 tcatgatgct gggcgctatc ggcctggtgt tctggatggc gaacagtctc ggttgggctg
     5401 ataccaacgt tgctgcgacc tattcgttga cgctgttatt cctgcgtacg ccgctgcttt
     5461 cggcggttgg cgcattgccg acgctgctga cggcgcaggt ggcgtttaac aagctgaaca
     5521 aattcgcgct cgcgcctttc aaagctgagt ttccgcgccc gcaggcgttt cccaactggc
     5581 aaacgctgga gctgcgtaac gtgacgtttg cttatcagga taacgcgttt tccgttggtc
     5641 cgattaatct caccatcaaa cgtggcgagc tgctgtttct gattggcggt aacggtagcg
     5701 gaaaatcgac gctggcgatg ttgttgacgg gcttgtatca gccacaaagc ggcgaaatct
     5761 tgctggatgg caaacctgtc agcggcgaac aaccggaaga ttatcgcaaa ctgttttcgg
     5821 cagtgtttac cgatgtctgg ctgtttgatc aactgctggg gccggagggg caacccgcta
     5881 acccgcaact ggttgagaag tggctggcgc agctgaaaat ggctcataag cttgagttaa
     5941 gcaacgggcg tattgttaac ctgaagttat caaaagggca gaaaaaacgc gtggcgctgt
     6001 tgctggcgct ggcagaagaa cgcgatatta tcctgctgga tgaatgggcg gtggatcagg
     6061 atccacactt ccgtcgtgag ttttatcagg tgttgctgcc gctgatgcag gagatgggta
     6121 aaactatttt cgctatcagt catgatgatc attactttat ccacgccgac cgcctgctgg
     6181 aaatgcgcaa tgggcaactt agcgagctga cgggcgaaga gcgcgatgcc gcttcgcgtg
     6241 atgccgttgc ccggacggca taatattacg acgctgacct ggcggactgc tgccgtcagg
     6301 tcaatatgct aaaaatccct tcatttctaa taaccctata attaatcaac gaaattataa
     6361 tgtttctaaa attagaatat aatttataaa cattatttaa atgttgttac ttaagtgtta
     6421 ccgttgatgc cgcgcaaatc tcacctgcaa taaagcgact aaaagtaagg cattaacaag
     6481 atgaaaaaag tgactgccat gctcttctcg atggccgtgg ggcttaatgc cgtttcgatg
     6541 gcggcaaaag cgaaagcgtc cgaggagcag gaaactgatg tactgttgat tggcggcggc
     6601 attatgagcg ccacgttggg gacctattga cgcgagctgg agcctgaatg gtcgatgacc
     6661 atggtggagc gcctggaggg gggcgcgcag gagagttcga acggctagaa caacgccgga
     6721 accgggcatt ctgcactgat ggaactgaac tacaccccgc aaaacgccga tggcagcatc
     6781 agtattgaaa aagcagtcgc cattaacgaa gcatttcaga tttcccgcca gttctgggcg
     6841 caccaggtcg agcgcggcgt gctgcgtact ccgcgttcat ttatcaatac cgttccgcat
     6901 atgagctttg tctggggcga ggataacgtt aatttcctgc gcgcccgtta cgccgcgttg
     6961 caacaaagct cgctgtttcg cggtatgcgt tactctgaag atcacgcgca gatcaaagag
     7021 tgggcaccgt tagtgatgga agggcgcgat ccgcaacaga aattggcact gatgtgaact
     7081 atggtgagat cacccgccag ttaattgctt ccttacagaa gaaatctaac ttctcgctgc
     7141 aactcagcag cgaagtccgc gccctaaagc gtaatgacga taacacctgg accgttaccg
     7201 ttgccgatct gaaaaacggc actgcacaga acatccgcgc caaatttgtc tttatcggcg
     7261 cgggcggtgc ggcgctgaag ctgttacagg aatcggggat tccggaagcg aaagactacg
     7321 ccggtttccc ggtgggcgga cagttccttg tttcggaaaa tccagacgtg gttaatcgcc
     7381 atctggcgaa agtttacggt aaagcatccg ttggcgcacc accgatgtcg gttccgcata
     7441 tcgatacccg tgttctggac ggtaaacgcg tagtgctgtt tgggccattt gccaccttct
     7501 caaccaaatt cctcaaaaac ggttcattgt gggatctgat gagttccacc accacctcta
     7561 acgtgatgcc gatgatgcac gtcgggctgg ataatttcga tctggtgaaa tatctggtga
     7621 gtcaggtgat gttgagtgaa gaggatcgtt ttgaagcgtt gaaagagtac tatccgcaag
     7681 cgaaaaaaga ggactggcgt ttgtggcaag cggggcagcg tgtgcagatt atcaagcgtg
     7741 atgccgagaa aggtggcgta ctgcgtctgg gtaccgaagt ggtcagtgac cagcaaggga
     7801 ccattgcggc gctgctgggg gcatcgccag gggcgtcaac cgccgcgccg attatgttga
     7861 atctactgga aaaagtattt ggcgatcgcg tttccagccc gcaatggcag gctacgttga
     7921 aagcgatcgt tccgtcttat ggacgcaagc tgaacggtga tgtagcggca acagaacgcg
     7981 agttgcagta caccagcgaa gtgctggggc tgaaatacga caaaccgcaa gcagcagata
     8041 gtacgccgaa accgcagttg aaaccgcaac ccgttcaaaa agaagtggcg gatattgcgt
     8101 tgtaatgatg tggcgtatac tgataagacg cgccagcgtc gcatcaggca accggctcgg
     8161 gcgttagatg ttggatgcgg cgtaaccgcc ttatccaacc aacggggtgc cgtacttcac
     8221 aacagtttag cgaacttccg cgttgtcaat tttctcttcc gccttccaga cgcggtattt
     8281 cacatctaca ttgtctggcg tatacaccac gatcggcagc ttgctgttgt aacgcagcat
     8341 tccagcatcg cccagatacg cggtgacaaa tttcttctct ttcttgccat ccgggcaggc
     8401 catcatcgtg gaaaccggcg agctaacttt atcaaagaca taataatcat agccccagcc
     8461 ttccagcgtt ttgctttcca gcttcccgcc gagacgatgc aaattgcaat cgacttccag
     8521 cgtctgaccg attaacagtt ctactttcag ggtagattca tcttcttgcg gggttaactg
     8581 aatcacctga cgcttcatgc ctgtttcagc ttgtggataa ggcgcgattt tttccagtgg
     8641 ctggacgctt tctgccgccc aggcggaagt ggtagcgaaa gcggcaaaca atacttcagg
     8701 tagaatggtc ttcatttttt tgctttcctt cttcatttat aggttttcac cgatgatgtc
     8761 gctgaaggtt aacatat
//
LOCUS       DAZPEU010000158         8760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPEU010000158 DAZPEU010000000
VERSION     DAZPEU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.159"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHU2L_003221"
     CDS             <1..439
                     /locus_tag="ACHU2L_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR1357965.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHU2L_003222"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHU2L_003222"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR1357966.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHU2L_003223"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHU2L_003223"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR1357967.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHU2L_003224"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHU2L_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR1357968.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="ACHU2L_003225"
     CDS             complement(4441..5148)
                     /locus_tag="ACHU2L_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFR1357969.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPGFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHU2L_003226"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHU2L_003226"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR1357970.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="ACHU2L_003227"
     CDS             complement(6006..6209)
                     /locus_tag="ACHU2L_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1357971.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..7876
                     /locus_tag="ACHU2L_003228"
                     /pseudo
     CDS             6368..7876
                     /locus_tag="ACHU2L_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..>8760
                     /locus_tag="ACHU2L_003229"
     CDS             7873..>8760
                     /locus_tag="ACHU2L_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR1357972.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2032 a         2389 c         2251 g         2088 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa acccaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
//
LOCUS       DAZPEU010000159         8681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPEU010000159 DAZPEU010000000
VERSION     DAZPEU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.160"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..956)
                     /locus_tag="ACHU2L_003230"
     CDS             complement(<1..956)
                     /locus_tag="ACHU2L_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR1357973.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACHU2L_003231"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACHU2L_003231"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR1357974.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHU2L_003232"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHU2L_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHU2L_003233"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHU2L_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR1357975.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACHU2L_003234"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACHU2L_003234"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR1357976.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACHU2L_003235"
     CDS             complement(5018..6346)
                     /locus_tag="ACHU2L_003235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1357977.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8681)
                     /locus_tag="ACHU2L_003236"
                     /pseudo
     CDS             complement(6354..8681)
                     /locus_tag="ACHU2L_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         2336 a         2170 c         1915 g         2260 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt c
//
LOCUS       DAZPEU010000160         8679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPEU010000160 DAZPEU010000000
VERSION     DAZPEU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.161"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(42..134)
                     /locus_tag="ACHU2L_003237"
                     /pseudo
     CDS             complement(42..134)
                     /locus_tag="ACHU2L_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACHU2L_003238"
     CDS             complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACHU2L_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR1357978.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="ACHU2L_003239"
     CDS             complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="ACHU2L_003239"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR1357979.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="ACHU2L_003240"
     CDS             complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="ACHU2L_003240"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR1357980.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="ACHU2L_003241"
     CDS             complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="ACHU2L_003241"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR1357981.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5304..7850
                     /gene="cyaA"
                     /locus_tag="ACHU2L_003242"
     CDS             5304..7850
                     /gene="cyaA"
                     /locus_tag="ACHU2L_003242"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR1357982.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="ACHU2L_003243"
     CDS             complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="ACHU2L_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR1357983.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8258..>8679)
                     /locus_tag="ACHU2L_003244"
     CDS             complement(8258..>8679)
                     /locus_tag="ACHU2L_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR1357984.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREGNVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1975 a         2241 c         2235 g         2228 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt
      361 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
      421 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
      481 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      541 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      601 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      661 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      721 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      781 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      841 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      901 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      961 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
     1021 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
     1081 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
     1141 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
     1201 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
     1261 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
     1321 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1381 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1441 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1501 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1561 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1621 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1681 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1741 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1801 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1861 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1921 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1981 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     2041 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg
     2101 ttgtccccgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc agtttttcca
     2161 gcatcgcctg gctttgcagg gtttccggaa gatccatcga gatattttgc tggcttaact
     2221 ggtccacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag taagcacgta
     2281 cccaggtgga gacgttctcc agcgcctggc gataagtctc ttcctgatga cgcggtacag
     2341 cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg cagatagata tcctgatttg
     2401 gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa
     2461 agttctgcca gcttttttgc agattgatac gccattcgct gatggaactg gaaagctctt
     2521 caccgtctga atccatcggc gaaccatcgc tgtcattatc ggccagacgc aggttatcta
     2581 cctgatttga aagctgatta agcttaagga tgatgccgtc ataatccacc tgcgatactg
     2641 cagaaaggct ggcgatatca tcggtaattg cccgacgaac ggtaatcaga ctcgggtcat
     2701 tcatatccgc caggctggcg tctgcacttt tcagcaacgc tgcagaggtc gtgacgtcct
     2761 gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctga gccagcagcc
     2821 aggttttagc atcgctgccg gaaatggtgg cgatcttttg ttggacttca tccaactgtt
     2881 ttgccagcgt ttcttgctga cgattcgcct gcttaagctg cgcagcttgt tgcttaataa
     2941 tgccttccag ctcggctttt tggctctcct gggctttttg caatgccgtc agttggttag
     3001 ccagggcatc gctggtggcg gtctgattga cggcctgttg tttaccccag ccatacaaac
     3061 cgacacccgc cgccagagca atagcgatag ccaccgcgct gagaatcaat gcagtattgt
     3121 tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc tccctggtct
     3181 cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt
     3241 attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc
     3301 agcccagttc ccgggcgagt ttcgccaaac gctcactgac gaccaatagt cgacagtgta
     3361 gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac
     3421 cgctggtaac aacgaccatc gtcacctcgc gggattgcca gcgcatcgct tcttctgcac
     3481 catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgca
     3541 ccgtcagggt atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacgtt
     3601 tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct
     3661 gtgggtagag aatcttctgt ccgcttacgg tatgtagcgc cagtgcggtg gtgcgtccaa
     3721 tggcgaaata agtaggtagt tggggccatt tacgatcttg ctgatgcagc tgtgattggg
     3781 caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctccccc agcgctgcca
     3841 gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc
     3901 aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctccagcg ggagacgggc
     3961 gggtgaccag gatactcatg ccggggcctc tccgttatag acttcagcga ggatctcgcg
     4021 cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg
     4081 cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga ccagcgcaca
     4141 tagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct gacatccgcc
     4201 ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt cgtggtgatt
     4261 cagcgcggca agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa taccgactgc
     4321 accttgtcct accgccggaa gagaaatctc gggtggtaac gcggcgcgaa tgcgggactc
     4381 cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcgtatt cgccgttatc
     4441 tagtttgctc aggcgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg
     4501 gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgctgg
     4561 taacgcatcc agactgtcat agttattgga cacaaaggca tcgcgaggat cttcacgctc
     4621 gcaaatagtg accagcccca gaccttgcgg gaattcaacc ggcacatctt tcattgaatg
     4681 tacggcgata tcagcgcgat tctccaggag cgcgacttcc agctctttta caaataagcc
     4741 ttttccgcct actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg
     4801 taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc
     4861 ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct
     4921 tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagtg atgtcagtgt
     4981 tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac
     5041 aggcgtttca cgccgtcgta ataaggaatt tacagagaat aaacggtgct acacttgtat
     5101 gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca
     5161 ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca
     5221 tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac agtcatgacg
     5281 ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg
     5341 atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac
     5401 aggtctacag tctactgccg acattgttgc actatcacca tccgcgaatg ccgggttacc
     5461 ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact
     5521 acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggatccaccg aaaggtgagc
     5581 ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct
     5641 ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttac
     5701 tacaacgtaa atgcagcctg ctggaaagct gggccgcctc gctgggtgtg gaagtcagct
     5761 tcttcctgat tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg gggggcgaag
     5821 attgtggctc cacccagcat atactgctgc ttgacgaatt ttatcgtact gccgtgcgtc
     5881 tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgacg
     5941 actatgtgat gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg ctggatctcg
     6001 gtggcttaag ctcgctttct gctgaagagt actttggtgc cagcctttgg cagctctaca
     6061 agagtatcga ttccccatac aaagcggtgc tgaaaacact gctgctggaa gcctattcct
     6121 gggaataccc gaacccacgt ctgctggcga aagatatcaa acagcgtttg cacgacggcg
     6181 agattgtatc gtttggcctc gatccatact gcatgatgct ggagcgtgtt actgaatacc
     6241 tgacggcgat tgaagatttc acccgtctgg atttagtacg tcgctgcttc tatttaaaag
     6301 tgtgcgaaaa actcagccgt gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc
     6361 agttagtgag cgagtggggt tgggacgaag ctcgtctggc aatgctcgat aaccgtgcta
     6421 actggaagat tgatcaggtg cgtgaggcgc acaacgagct gctcgacgcg atgatgcaga
     6481 gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc gccagtccgc
     6541 aggatatcgg cgtactgacg cgtaaactgt atgccgcgtt tgaagcattg ccaggtaaag
     6601 tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg acctttattt
     6661 atgtgccgcc gggccgggct aaccgttcag gttggtatct gtataaccgc gcgccaaata
     6721 ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat aaactggtgg
     6781 cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg tttgtatatt aaaggtaacg
     6841 gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac catttcccgc
     6901 tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatt cgccatctgg
     6961 cgattatcgt taacctggaa tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt
     7021 ttgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta
     7081 gcgttgacct gctgtaccgc aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg
     7141 agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac
     7201 cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta atccgtactc
     7261 gcgtgcagca actggtttct gagtgtattg aactgcgcct ttccagcacc cgccaggaaa
     7321 ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc ttcgaacgcc
     7381 tgaatgtatc ggtacagaag ctggagaacg ccatcgagtt ttatggcgct atttcgcata
     7441 acaaactgca cggcttgtca gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg
     7501 tggacggctt tgccagcgaa gggatcatcc agttcttttt cgaagaaacg caagacgaga
     7561 atggctttaa tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg
     7621 aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt ctactcgtca tcgcacgacc
     7681 gcttcaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat cagattgtga
     7741 aggttgatgg tcgcgaacag gtgattccgt tccgaacaaa atcgattggc aacctgccgc
     7801 ccgccaatca ggatcacgat acgccgctat tacagcagta tttttcgtga tgagcgtgcc
     7861 ggaaagcgag gcttatccgg catgcaatct tagcggaaac tgactgtttc acccgcctgt
     7921 tgcgtcgccg cctgttccag caaatcccag aaggtttcgc cgctgcgatc gcaaatccat
     7981 tcatcgcctt tcaggtcaaa atggtagccg ccctgtttgg ttgccagcca cacctggtgc
     8041 agcggctcct ggcggttaat aatgattttg ctgccgtttt caaaggtaat ggtcagtacg
     8101 ccgccgttga tttcgcagtc gatatcgctg tcgccatccc tgtcgtccag acgctcttca
     8161 atggtcagcc acagttgatc agccaggcga tgaaattcac tgtcgttcat tgttgtatcc
     8221 tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa gtttgcacct
     8281 ctgctgctgc gttccggcat gattcatccg tcaccggaat aatgatgtct ctgttttgaa
     8341 cttcacgtac gttcccctct ctgacacgag ttttcgccgg gcagggggag ggcgtaatcc
     8401 ttacctctgt atcggtccat gtataaacat tgcgatcgtc ataagcgaag cgatcgtttt
     8461 tagttagcga accgttaact ggaataattt gcaagctctt tgaccgtaat tggcggataa
     8521 agtaagcatg ctgtttgccg ctattaaatg agcgatcaat gaccaggtct ggcgtatcca
     8581 gcgggattat ctcaagctgg tcttcaccaa atgtcgataa ttttgttaca tacagccgat
     8641 ctttatattg cgcaatattg ccagtaatca gatggaatt
//
LOCUS       DAZPEU010000161         8677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPEU010000161 DAZPEU010000000
VERSION     DAZPEU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.162"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            105..359
                     /gene="yjbL"
                     /locus_tag="ACHU2L_003245"
     CDS             105..359
                     /gene="yjbL"
                     /locus_tag="ACHU2L_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR1357985.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            383..1090
                     /locus_tag="ACHU2L_003246"
     CDS             383..1090
                     /locus_tag="ACHU2L_003246"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1357986.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            1453..2490
                     /gene="dusA"
                     /locus_tag="ACHU2L_003247"
     CDS             1453..2490
                     /gene="dusA"
                     /locus_tag="ACHU2L_003247"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR1357987.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2624..2866
                     /gene="pspG"
                     /locus_tag="ACHU2L_003248"
     CDS             2624..2866
                     /gene="pspG"
                     /locus_tag="ACHU2L_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR1357988.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(3032..4015)
                     /locus_tag="ACHU2L_003249"
     CDS             complement(3032..4015)
                     /locus_tag="ACHU2L_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR1357989.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            4098..5513
                     /gene="dnaB"
                     /locus_tag="ACHU2L_003250"
     CDS             4098..5513
                     /gene="dnaB"
                     /locus_tag="ACHU2L_003250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1357990.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5566..6645
                     /gene="alr"
                     /locus_tag="ACHU2L_003251"
     CDS             5566..6645
                     /gene="alr"
                     /locus_tag="ACHU2L_003251"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR1357991.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6898..8091
                     /gene="tyrB"
                     /locus_tag="ACHU2L_003252"
     CDS             6898..8091
                     /gene="tyrB"
                     /locus_tag="ACHU2L_003252"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR1357992.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(8594..>8677)
                     /locus_tag="ACHU2L_003253"
     CDS             complement(8594..>8677)
                     /locus_tag="ACHU2L_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1357993.1"
                     /translation="KLQKIFVDLEKKYQVLLQVKFFTHLLI"
BASE COUNT         2212 a         1985 c         2176 g         2304 t
ORIGIN      
        1 aaacgcatac ctgtagttca gaaattatga gcgatttttg atagtgacca gatggcaaaa
       61 gaattataag gaggacacgg tacgacaaaa aaggtagatg aatcatgttg aagataatac
      121 ccggtgcaac tggatacttt aataaaacac tcaattcaaa tcaattcgaa aataaagatg
      181 caaccaaaga taaattggat aatagaggtt caatcaaagg taaattaaat aatatatatg
      241 gtaaatctat tgattatgca gcgctgcgtc atcgggatat aattattgca aaaatagatt
      301 tgtttattca acgaatcacg cataatttat ggcatgcacg taaaaaaatg tgtttttaat
      361 tgagcacata aatgatctca aaatgtgggt caataagtat actgacgatt gcactgatga
      421 ggatttaaac gatcgtgact ttattgcatc agttgttgac cgggctattt ttcatttcgc
      481 gattaatagt atatgtaatc ctggggataa taaagatgca atgcccattg aacaatgtac
      541 ttttgatgta gaaactaaga atggccttcc ctccacggtt cagctatttt atgaggaatc
      601 taaggataat gaacctttag cgaatataca ttttcaagca ataggttctg gttttttaac
      661 gtttgttaat gcctgccagg aacatgatga caacagctta aaattatttg cttcgctgtt
      721 aatttcactt tcatattcta gtgcctacgc agatttatca gaaacagtgt atattaatga
      781 aaataatgag agctacctga aagctcagtt tgaaaaatta tatcaacgtg atatgaagaa
      841 gtacctggga gagatgaagc gtctggcaga tgggggagaa atgaattttg atggctatct
      901 ggataagatg tcacatctgg tgaatgaagg aacgctcgat cctgatattt taagcaaaat
      961 gcgagatgct gcaccacaat taattagctt cgcgaagtcg tttgacccaa cctcaaagga
     1021 agagattaaa atacttacag acacttctaa attaatttat gacttgttcg gggttaaatc
     1081 ggagaaataa tatgtgaagt tcttcgatag tatggaaggc attatataaa aggacccaat
     1141 atttattggg ttcttttttc tctatcaatg ctattagcag ggagatatat caccagagtt
     1201 taatgtgtga ttttttattt atcgtcgaac ctggattgtt tatcattggc cttaacaaag
     1261 ttaacggcta ataagattat ttccatcact tcgtgagagc ttcatgcctt gagaggatct
     1321 caattttctt ttgcaatgag acaggcgctt cctgttgtta tggtatagta ccccgctatt
     1381 gagcctcctg aacagtgaag ctgaataaca taaccccatg atatatcgat aaaataatct
     1441 ctacatttga aaatgcacgg taattctgaa atgcaaaaaa tcaaccaaac cagcgcaatg
     1501 cctgaaaaaa ctgacgttca ctggagtggt cggtttagtg ttgcaccaat gctcgactgg
     1561 acggacagac attgccgcta tttcttgcgt ctgctttccc gcaatacgtt gctttatacc
     1621 gaaatggtga ccacaggggc gataattcac ggtaaaggtg attatctggc gtacagtgaa
     1681 gaagaacatc cggtagcgtt gcaactgggc ggtagcgatc cggcggcgct ggcgcagtgt
     1741 gcaaagctgg cagaagcgcg cggatatgat gagatcaacc tgaatgtcgg ctgcccgtct
     1801 gaccgggtgc agaacggcat gtttggtgcg tgtctgatgg gtaatgcgca gctggttgcc
     1861 gactgcgtga aagcgatgcg cgatgtggtg tcgattccgg tgacggtgaa aacgcgtatt
     1921 ggcatcgacg accaggacag ctatgaattt ctctgcgatt tcatcaatac cgtttccggc
     1981 aaaggcgagt gtgagatgtt catcatccac gcacgtaaag cctggctttc ggggttaagc
     2041 ccgaaagaaa accgtgaaat cccgccgctc gattatccgc gtgtgtatca actgaagcgt
     2101 gacttcccgc atctgacaat gtcgattaac ggtggtatca agtcgctgga agaagctaaa
     2161 gcgcatttgc aacatatgga tggcgtgatg gtcgggcgcg aggcgtatca gaatccgggt
     2221 attctggcgg cggtagaccg ggagatcttt ggttcctcgg ataccgatgc cgatccggtg
     2281 gcagtagtgc gtgccatgta tccgtacatt gagcatgaac tcagccaggg gacgtatctc
     2341 ggccatatta tccggcatat gctgggtttg ttccagggta ttcctggcgc gcggcagtgg
     2401 cggcgttatt taagtgaaaa tgcccataaa gcgggtgctg acattaacgt gctggaacac
     2461 gcgctcaaac tggtggcgga taagcgttaa cttttcacca aaaagtagtc aaattcacca
     2521 cgccctgcgc accgtcgcgg ggcgttttgc tgttaaatca atagattatt tttggcatga
     2581 ttcttgtaat gccagcaaga gatttcatat ttgggagagc atcatgctgg aactactttt
     2641 tgtgattggc ttttttgtca tgctgatggt caccggcgtt tcgttgctgg gcattatcgc
     2701 cgcgctggtt gtggcgacgg ccattatgtt cctcggcggt atgctggcat tgatgattaa
     2761 gttgctgccg tggttactac tggcgattgc ggtggtgtgg gttattaagg cgattaaagc
     2821 accaaaaatg ccgaaatatc agcgttatga ccgctggcgt tactaaggga ttgtgcggat
     2881 gatcacaacc taaggtttta tccttagaac aaaataggaa ttgataatca aatctgtcac
     2941 tattgcgcct ctaacagatt catcgtgctg taccctacat acagccgaac tataaaaaga
     3001 aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg gaaccttgcg
     3061 tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga tatttctgct
     3121 gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt tcattactgg
     3181 cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt gtcacataca
     3241 acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct gatgagttgc
     3301 caaaactgac cattaagccg cggcgttgca ggcaatccag cgaccgttcc caggtgtctc
     3361 tgcccacgga atcgtacact acgcgcactt tcttaccgcc ggtgatctct ttcaatcgct
     3421 cgaccagatt ctcttcacga tagttaataa cctgccacgc gccggctttt agcgcgctct
     3481 gcgctttttg cgcggttcct acggtgccga tgagtttcgc gcccagggct tttgcccact
     3541 ggcaggcaat taagccaatg ccgccagccg ctgcgtggaa caggaactgt tcatcgggtt
     3601 taatttcata ggttttgcgc agcagataat aaaccgttaa gcctttcagg aaggatgccg
     3661 cagcttgctc aaaagaaatt gccgcaggca gaatcgccgc tttatccgca ttaatgttat
     3721 gcacagagct gtaagcgcct aacgccgact gcgcatagac tacatgatcg ccagccttaa
     3781 tatgctttac accactgccg actttactca cgatgcctgc cgcttcggta cctaatccgc
     3841 tgggtagcga tggcggcggg taaaggccgc tgcggatata cgtgtcgata aaattgatgc
     3901 cgatggcttt attttcgacc tggatttcat tctccgccgg atcggcagga gtgaactcta
     3961 cggcttgaag tacttccggg ccaccgtgct tgtgaaattc aattcgtgtt gccatgtgtc
     4021 ctccagaacg tatcgtcagg gtctgcttca tatggtaaag tttcgaccca ttctttattt
     4081 cggtaactcc attcactatg gcaggaaata aacccttcaa caaacagcag gctgaacccc
     4141 gcgaacgcga tccacaagtt gccgggctga aagtgcctcc gcactcgatc gaagcggagc
     4201 agtcggtgtt gggcggttta atgctggata acgaacgctg ggatgatgta gccgagcgtg
     4261 tggtagcaga cgatttttac acccgcccac accgtcatat ctttactgaa atggcgcgtt
     4321 tgcaggaaag cggtagtcct atcgatctga ttacccttgc ggaatcgttg gaacgccagg
     4381 ggcaactcga tagcgtcggt ggttttgctt atctggcaga gctgtcaaaa aatacgccaa
     4441 gtgcggcgaa catcagtgct tatgcggaca tcgtgcgtga acgtgccgtt gtccgtgaga
     4501 tgatctcggt tgcgaatgag attgctgaag ccggttttga tccgcagggg cgtaccagcg
     4561 aagatctgct ggaccttgct gaatcccgcg tctttaaaat tgccgaaagt cgtgcaaaca
     4621 aagacgaagg gccgaagaac atcgccgatg tgctcgacgc aaccgtggcg cgtattgagc
     4681 agttgtttca gcagccacac gatggcgtta ccggggtaaa caccggttat gacgatctca
     4741 acaaaaaaac cgctggcttg cagccgtcgg atttgatcat cgtcgccgcg cgtccgtcga
     4801 tgggtaaaac aacatttgcg atgaacctcg tcgaaaacgc ggcaatgttg caggataaac
     4861 cagtacttat cttctcgctg gagatgccat cagaacagat catgatgcgt tctctggcgt
     4921 cgctgtcgcg cgttgaccag actaaaatcc gtaccgggca gctcgatgat gaagactggg
     4981 cgcgcatttc cggcaccatg ggtattttgc tcgaaaaacg caatatctat atcgatgact
     5041 cttccggcct gacgccaacg gaagtgcgtt cccgcgcacg ccgtattgcc cgtgaacacg
     5101 gcggcatcgg gcttatcatg atcgactacc tgcaactgat gcgcgtaccg gcgctttccg
     5161 ataaccgtac gctggaaatt gcagaaatct cccgctcgct gaaagcactg gcgaaagaac
     5221 tgaacgtgcc ggtggtggcg ctgtcccagt tgaaccgttc tctggaacaa cgtgccgaca
     5281 aacgtccggt caactccgac ctgcgtgaat ctggctctat cgagcaggat gcggacttga
     5341 tcatgtttat ctatcgtgat gaggtgtatc acgaaaacag tgatttaaaa ggcatcgcgg
     5401 aaattattat cggtaaacaa cgtaacggcc caatcgggac ggtacgcctg acctttaacg
     5461 gtcaatggtc gcgcttcgac aactatgcgg ggccgcagta cgacgacgaa taataattat
     5521 tttatgaatt aggtaattaa agcaaacaat tatcaaggaa cacaaatgca agcggcaact
     5581 gttgtgatta accgccgcgc tctgcgacac aacctgcaac gtcttcgtga actggcacct
     5641 gccagtaaaa tggttgcggt ggtgaaagcg aacgcttatg gtcacggtct tcttgagacc
     5701 gcgcgaacgc tccccgatgc tgacgccttt ggcgtagccc gtctcgaaga agctctgcga
     5761 ctgcgtgcgg ggggaatcac caaacctgta ctgttactcg aaggcttttt tgatgccaga
     5821 gatctgccga cgatttctgc gcaacatttt cataccgccg tgcataacga agaacagctg
     5881 gctgcgctgg aagaggctag cttggacgag ccggttaccg tctggatgaa actcgatacc
     5941 ggtatgcacc gtctgggcgt aaggccggaa caggctgagg cgttttatca tcgcctgacc
     6001 cagtgcaaaa acgttcgtca gccggtgaat atcgtcagcc attttgcgcg cgcggatgaa
     6061 ccaaaatgtg gcgcaaccga gaaacaactc gctatcttta ataccttttg cgaaggcaaa
     6121 cctggtcaac gttccattgc cgcgtcgggt ggcattctgc tgtggccaca gtcgcatttt
     6181 gactgggtgc gcccgggcat cattctttat ggcgtctcgc cgctggaaga tcgctccacc
     6241 ggtgccgatt ttggctgtca gccagtgatg tcactaacct ccagcctgat tgccgtgcgt
     6301 gagcataaag ccggagagcc tgttggttat ggtggaacct gggtaagcga acgtgatacc
     6361 cgtcttggcg tagtcgcgat gggctatggc gatggttatc cgcgcgccgc gccgtccggt
     6421 acgccagtgc tggtgaacgg tcgcgaagta ccgattgtcg ggcgcgtggc gatggatatg
     6481 atctgcgtag acttaggtcc acaggcgcag gacaaagccg gggatccggt cattttatgg
     6541 ggcgaaggtt tgcccgtaga acgtatcgct gaaatgacga aagtaagcgc ttacgaactt
     6601 attacgcgcc tgacttcaag ggtcgcgatg aaatacgtgg attaatcgtt ctgtaatatt
     6661 tgattgtctg tgccggatgc ggcgtgaatg ccttatccgg ccaataaaat cctaaaaatt
     6721 caataagttg atgttctttc atgctcttat aaaggtcgtg cctctggcgg atgtacgttt
     6781 atcattagtc tcactctgtt gctaattgcc gttcgctcct gaacatccac tcgatcttcg
     6841 ccttcttccg gtttattgtg ttttaaccac ctgcccgtaa acctggagaa ccatcgcgtg
     6901 tttcaaaaag ttgacgccta cgctggcgac ccgattctta cgcttatgga gcgttttaaa
     6961 gaagaccctc gcagcgacaa agtgaattta agtatcggtc tgtactacaa cgaagacgga
     7021 attattccac aattgaaagc cgtggcggag gcggaagcgc gcctgaatgc gcagccccat
     7081 ggcgcttcgc tttatttacc gatggaaggg cttaacagct atcgccatgc cattgcgccg
     7141 ctgctgtttg gtgcggacca tccggtactg caacaacagc gcgtagcaac cattcaaacc
     7201 cttggcggct caggggcatt gaaagtgggc gcggatttcc tgaaacgcta cttcccggaa
     7261 tcaggcgtct gggtcagcga tcctacttgg gaaaaccacg tagcaatatt cgccggggct
     7321 ggattcgaag tgagtactta cccctggtat gacgaagtga ctaacagcgt gcgctttaat
     7381 gacctgttgg cgacgctgaa aacattacct gcccgcagta ttgtgttgct gcatccatgt
     7441 tgccacaacc caacgggtgc cgatctcact aatgatcagt gggatgcggt gattgaaatt
     7501 ctcaaagccc gcgagcttat cccattcctc gatattgcct atcaaggatt tagtgccggt
     7561 atggaagagg atgcctacgc tattcgcgcc attgccagtg ctggattacc cgctctggtg
     7621 agcaattcgt tctcgaaaat tttctccctt tacggcgagc gcgtcggcgg actttctgtt
     7681 ctgtgtgaag atgccgaagc cgctggccgc gtactggggc aattgaaagc aacagttcgc
     7741 cgcaactact ccagcccgcc gaattttggt gcgcaggtgg tggctgcagt gctgaatgac
     7801 gaggcattga aagccagctg gctggcggaa gtagaagaga tgcgtactcg cattctggca
     7861 atgcgtcagg aattggtgaa ggtattaagc acagagatgc cagaacgcaa tttcgattat
     7921 ctgcttaatc agcgcggcat gttcagttat accggtttaa gtgccgctca ggttgaccga
     7981 ctacgtgaag aatttggtgt ctatctcatc gccagcggtc gcatgtgtgt cgccgggtta
     8041 aatacggcaa atgtacaacg tgtggcaaag gcgtttgctg cggtgatgta atgcaggaaa
     8101 gcaggctgga gttacccagc ctgcagtgaa attaaactgt cgtcgctttc actatttctt
     8161 tgtagatgat ttttttgatg ccgttgttct acgtgagaga taataaaacg ttgtcagttc
     8221 ttttattgtt aagtttatcc caattatctg gaattccttt ccctattttt tcgtgtggag
     8281 taataatttc tgcacatcta accattatta aatttaataa taagtatcgg gtttgttacg
     8341 atagaatatt tctatatcct caagaatatg ctttgcctta ttgcctgtga ctttctgaga
     8401 ttcaattata gtatcgttaa attctaatgt taaagagaac tcttttttcc gctttgtgta
     8461 agtttaacct tatattgatt accaatatca aataaaaagg tcctgtaatg agaataatca
     8521 cctttaacct cctcggcaaa acagcaaagc gtgcaaatat acataaagtc tttcttaccc
     8581 ctgattttgt taactaaatc aataaatgcg tgaaaaactt tacttgcaat agaacttgat
     8641 acttcttctc caggtctaca aaaatctttt gcaattt
//
LOCUS       DAZPEU010000162         8641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPEU010000162 DAZPEU010000000
VERSION     DAZPEU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.163"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(348..554)
                     /locus_tag="ACHU2L_003254"
     CDS             complement(348..554)
                     /locus_tag="ACHU2L_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR1357994.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            867..956
                     /gene="kdpF"
                     /locus_tag="ACHU2L_003255"
     CDS             867..956
                     /gene="kdpF"
                     /locus_tag="ACHU2L_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR1357995.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            956..2629
                     /gene="kdpA"
                     /locus_tag="ACHU2L_003256"
     CDS             956..2629
                     /gene="kdpA"
                     /locus_tag="ACHU2L_003256"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR1357996.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2652..4402
                     /gene="kdpB"
                     /locus_tag="ACHU2L_003257"
                     /pseudo
     CDS             2652..4402
                     /gene="kdpB"
                     /locus_tag="ACHU2L_003257"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4708..5280
                     /gene="kdpC"
                     /locus_tag="ACHU2L_003258"
     CDS             4708..5280
                     /gene="kdpC"
                     /locus_tag="ACHU2L_003258"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR1357997.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5273..7953
                     /gene="kdpD"
                     /locus_tag="ACHU2L_003259"
                     /pseudo
     CDS             5273..7953
                     /gene="kdpD"
                     /locus_tag="ACHU2L_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7950..8635
                     /gene="kdpE"
                     /locus_tag="ACHU2L_003260"
                     /pseudo
     CDS             7950..8635
                     /gene="kdpE"
                     /locus_tag="ACHU2L_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1840 a         2232 c         2497 g         2072 t
ORIGIN      
        1 tttattgcgg tagcgatact aataacaaaa ttcggaattt atttagatgc tggaattaga
       61 aaaagggatg acggctttct caccttaccc tgaagtttca cgaaagtgtg taaggaagta
      121 tgcaatctaa ctggagattt ctgatgatct agtcgcttag agcgacaatg aaggtgaccg
      181 catcctgggt agccctatga aaggatacta ttgaaacgtg atgctaaaac agtgatgaaa
      241 tgaataatac gatggaaacg tcttccatgt taattatcat ggaagacgtt gatgttgaac
      301 acctaaacca gccccctgat acgaggctgg cttaagcgcg caggcaatca gtaaaaatca
      361 ccggttgcct tttcggcctg atccatccac accggtttat cgctggtttt cgaccagacg
      421 cggtgcaggt agctgtaaaa gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc
      481 agaacgcccg ctgcaaccgc atatgtacgg cgtaaaaaga taagccatgc aggatattct
      541 ctgtagagtt ccatactttt ctcccccttt tgtatctacc cggtgaatag caccggaaaa
      601 atgaatttgt ttatctgatg aaaatagtac cgccttttgt gtaattttac tactcatccg
      661 accacttatt tttgcttatt gatggtttat ttacattcac actgtaatta aattacacaa
      721 aagttaaatt aatactaacc atcagttaaa tcatggcctt tgccattttt atactttttt
      781 tacaccccgt ccgcagattt ttgcgaaatc tttgcagcca gaattctact cttccggtat
      841 cacttttagg ccactggagg tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg
      901 tgtttttatt actgggctat ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc
      961 tgcgcaaggg ttcttactga ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc
     1021 tttaggcagc gggctggcgc ggctgattaa tgacattcct cttccaggta cagcgggcgt
     1081 tgaacgtata ctctttcgtt tacctggcgt ctctgaccat gagatgaact ggaagcaata
     1141 tctttgtgcc attctcggcc tgaacatgct ggggctggcg gtgctgtttt ttatgttgct
     1201 cggtcagcac tatctgccgc ttaatccaca gcagttgccg gggctgtcgt gggatctggc
     1261 gctgaatacc gccgtcagct ttgtcacgaa taccaactgg caatcttata gcggtgaaac
     1321 cacgttgagc tatttcagcc agatggcagg cttaacggta caaaactttc tttctgccgc
     1381 cagcgggatt gcggtgattt ttgccttcat ccgtgcgttt actcgccaga gcatgagcac
     1441 actcgggaat gcctgggtcg atctgctacg catcacgtta tgggtgctag tccctgtggc
     1501 gttgttgatc gcactgtttt ttattcaaca aggtgcgcag caaaactttc tgccctatca
     1561 ggcagtgaat accgttgaag gagcgcaaca gctgttaccc atgggaccag tagcttctca
     1621 ggaagcgatc aagatgctcg gtactaacgg cggtggcttc tttaatgcca actcgtcgca
     1681 tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag atgctggcga tcttcttgat
     1741 cccaacagcg ctgtgctttg cctttggtga agtgacgggc gatcgccgcc aggggcgcat
     1801 gttgctgtgg gcgatgtcag tgatttttgt catctgcgta ggcgtggtta tgtgggcaga
     1861 agttcagggt aatcctcatc tgctggcgct cggcgcagac agcagcatca atatggaagg
     1921 taaagagagc cgtttcggcg tgctggtcag tagcctgttt gcggtcgtga ctacggcggc
     1981 gtcctgtggc gcggtgattg cgatgcatga ttcgtttacc gctctcggcg gcatggtgcc
     2041 gatgtggcta atgcaaattg gtgaagtggt gttcggcggt gtcggttctg gtctttacgg
     2101 catgatgctg tttgtcctgc tggcggtgtt tattgccggg ctgatgattg gtcgtacacc
     2161 ggaatatctg ggtaaaaaaa tagacgtacg cgagatgaaa ctgactgcac tggcgattct
     2221 ggttactcca acactggtac tgatgggcgc ggcgttggcg atgatgaccg acgccggacg
     2281 tagcgccatg cttaacccta gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcgtc
     2341 cgccgctaac aacaacggca gcgcctttgc cggattaagc gccaactctc cgttctggaa
     2401 ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc
     2461 aattgccggt tcgctggtga gtaaaaagag ccaacccgcc agctccggca cgctgccaac
     2521 gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg ttgctggttg gcgcactgac
     2581 ctttatccct gccctggcgc ttggtccggt ggcggaatat ctctcctgat gatattgagt
     2641 gagcactgaa tatgagtcgt aaacaactgg cgctattcga accaacactt gtcgttcagg
     2701 cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt
     2761 ttatcgtctg gatcggcagt ctgctgacca cctgtattag catcgcgatg gcaagcggtg
     2821 cgatgcctgg caatgcgctg tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac
     2881 tgttcgctaa tttcgccgag gcgctggcag aaggccgcag taaagcgcag gccaacagtc
     2941 ttaaaggggt gaaaaaaact gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg
     3001 cggcggacaa agttcctgcc gatcaactgc gtaaaggtga catcgtgctg gtagaagctg
     3061 gcgatattat cccctgcgat ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg
     3121 ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg cggcgatttt gcctccgtta
     3181 ccggcggcac gcgtattctt tctgactggc tggtgattga gtgtagcgtt aaccccggtg
     3241 agacatttct ggatcggatg atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga
     3301 acgagattgc cctgactatc ctgctgattg ctctgaccat cgtcttttta ctggcaaccg
     3361 ccacgctgtg gccgttttcc gcgtggggcg gtaatgccgt gagtgtaacg gtactggtgg
     3421 cgctgctggt ctgtctgatc ccaaccacta ttggtggcct gttgtcagcg atcggcgtcg
     3481 ccgggatgag tcggatgctg ggcgcgaatg tgattgccac cagcggacgt gcagttgaag
     3541 cggcaggtga cgttgacgtt ctgctactgg ataaaaccgg caccatcaca ctcggtaacc
     3601 gtcaggcgtc ggagtttatc cccgcgcagg gcgtggatga aaaaacgctg gctgacgccg
     3661 cacaactggc ttcgctggct gatgaaacgc cggaaggtcg cagtattgtg atcctcgcca
     3721 agcaacgttt taacctgcgc gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt
     3781 ttactgcgca aagccggatg agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt
     3841 ctgtcgatgc cattcgccgc catattgagg ctaacggcgg tcacttccct gccgatgttg
     3901 atcaaaaagt cgatcaggtt gcgcgtcaag gagccacgct gctggtggtg gtggaaggtt
     3961 ctcgtgtgct gggcgttatt gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct
     4021 tcgcccagct gcgcaaatgg gcattaaaac ggtgatgatt accggcgata accgtctgac
     4081 tgccgccgcg atagctgcgg aagcgggtgt cgatgatttt ctcgccgaag cgacaccgga
     4141 ggccaagctg gcattgatcc gtcagtatca ggcggaaggt cgtttggtag cgatgaccgg
     4201 cgacagcacc aacgatgcta cggcgctggc gcaggcagat gtcgcggtgg cgatgaactc
     4261 cggcacccag gcggctaaag aggcgggcaa tatggtggat ctcgactcta acccgaccaa
     4321 gttgatcgag gtggtgcaca ttggcaaaca gatgctgatg acccgtggct cgctgaccac
     4381 cttcagcatt gccaacgatg tggcgaaata cttcgccatt attccggcgg cgttcgcggc
     4441 aacatatccg cagttaaatg cgctgaacat catgcgcctg cattcgcccg actccgccat
     4501 tctcagtgcg gtcattttca acgccttgat tatcgtcttt ttgattcctc tggcgttaaa
     4561 aggcgtgagt tataaaccgc ttaccgcttc tgccatgttg cgccgtaact tatggattta
     4621 cggtctgggt gggctgctgg tgccgtttat tggtatcaaa gtcattgatt tactgctaac
     4681 cgtttgtagt ctggtgtgag gtttaccatg agtggattac gtccggcatt atcgacattt
     4741 ctctttctgt tattgattac tggcggcgtt tacccgctgc tgaccaccgc actggggcaa
     4801 tggtggtatc cctggcaggc caacggttcg ttgattcgtg aaggtgatac ggtgcgcggt
     4861 tcggcattaa tcgggcaaaa ttttaccggc aacggctatt ttcatggtcg cccgtcggca
     4921 acagcagaaa tgccctataa tccacaggct tctggcggga gcaatctggc agtcagtaac
     4981 ccggagctgg ataaacagat agccgcacgc gttgccgcat tacgggccgc taacccggac
     5041 gccagcacga acgttccggt tgaactggtg acggcgtcgg caagcggact ggacaataac
     5101 atcaccccgc aagcggcggc ctggcagatc ccacgcatcg caaaagcgcg taatctcagc
     5161 gttgaacagc tcacgcagct gatcgcaaaa tacagccaac aaccgctggt gaaatatatc
     5221 ggccagccgg tcgtcaacat tgtcgaactc aatctggcac tggataagct tgatgaataa
     5281 cgaaccctta cgtcccgacc ccgaccgtct gctggaacaa actgccgcgc cgcatcgggg
     5341 gaagctgaaa gttttcttca gtgcctgtgc aggcgtcggg aagacctggg cgatgctggc
     5401 agaagcccag cgactgcggg cgcaagggct ggatattgtg gttggtgtgg tagaaaccca
     5461 tgggcggaaa gataccgccg ccatgcttga agggctggct gttctgcctc tgaaacgcca
     5521 ggcgtaccgt gggcggcata tcagcgagtt tgatctcgat gccgccctcg cccgccgtcc
     5581 ggcgctgatc ttaatggacg aactggcgca cagtaatgcg ccaggttctc gtcatcctaa
     5641 acgctggcag gatatcgaag aactgctgga agctggcatt gatgttttca caaccgtcaa
     5701 cgttcagcat ctggaaagtc tgaatgatgt ggtcagcggc gtcaccggaa ttcaggtacg
     5761 ggaaaccgtg cccgatcctt ttttcgatgc cgccgacgac gtggtgctgg tggacttgcc
     5821 cccggacgat ctgcgccagc ggctgaaaga aggcaaagtc tatattgccg ggcaggcgga
     5881 gcgcgccatt gaacattttt tccgcaaagg caatctgatc gccctgcgcg aactggcact
     5941 gcgccgtact gccgatcgcg ttgatgagca aatgcgcgcc tggcgggggc atcctggcga
     6001 agagaaagtc tggcacacgc gcgacgcgat ccttttatgc atcggccata acaccggcag
     6061 cgaaaactgg tccgcgcggc ggcgcggctg acgtcacggc tggggagcgt ctggcacgcg
     6121 gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg ggcaattctc
     6181 agcgccttac gtctggcgca ggagctgggc gcggagacgg caacactttc tgatccagcg
     6241 gaagagaaag cggtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt
     6301 cgcccagcct cgcgccgctg gtggcgtcgg gaaacatttg ctgaccgact ggcgcgcatc
     6361 gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac
     6421 aacgcgccgg atagccgctc atttaaagac aagtggcggg tacagattca gggatgcgtg
     6481 gttgccgccg cgttatgcgc cgttatcacc ttaattgcca tgcagtggct gatggcgttt
     6541 gatgccgcca acctggtgat gctgtatctg cttggcgtgg tggtggcgct attttatgga
     6601 cgctggcctt cggtggttgc caccgtcatt aatgtggtga gtttcgatct cttttttatc
     6661 gccccacgcg gcacgctcgc cgtctctgat gtgcaatatc tgctgacctt cgcggtgatg
     6721 ttaaccgtcg ggctggtgat cgggaacctt actgctggtg tgcgttatca ggcgcgggta
     6781 gcccgttacc gcgagcaacg cacacggcac ttatatgaaa tgtcgaaagc tctggcggtg
     6841 ggccgcagtc cgcaggacat cgccgccacc agcgaacaat ttattgcctc cacgtttcat
     6901 gcccgcagtc aggtgttgtt gcccgatgac aacggtaaat tgcagccgtt aacacatccg
     6961 caaggaatta cgccatggga cgatgccatc gcgcagtgga gttttgataa aggcctgcct
     7021 gcgggcgcgg gcaccgacac gttacccggt gtaccgtacc agattttgcc gctaaaaagc
     7081 ggcgagaaaa cctacgggct ggtggtggtg gagccgggga atctgcgcca gttgatgatc
     7141 ccggaacagc agcgcctgct ggagactttt acgctgttag tcgccaatgc ccttgagcgg
     7201 ctgacgctaa ccgccagcga agaacaggcg cggatggcaa gcgaacgtga acagatccgc
     7261 aacgccctgc tggcagcgct ttcgcatgat ttacgcacgc cgcttacggt gctgtttggt
     7321 caggcagaaa tcttaacgct cgatctggca agcgaaggat caccccacgc ccgccaggcc
     7381 agcgagattc gtcagcatgt gctgaacact acccgactgg tgaataatct actggatatg
     7441 gcgcgaattc agtccggcgg ctttaatttg aagaaagagt ggttaacgct ggaagaagta
     7501 gtcggcagcg cgctgcaaat gccggaaccg ggtttatcgt cgcccatcaa tctttctctg
     7561 ccagaaccgc tgaccttaat ccacgttgac gggccactct ttgaacgggt gctgattaat
     7621 ctgctggaga acgcggtgaa atatgcgggt gcgcaggccg aaattggtat cgatgcccac
     7681 gttgagggcg aaaatctaca actggatgtc tgggataacg gccccggtct tccgccaggc
     7741 caggagcaga cgatatttga taagtttgct cgcgggaata aagagtcggc ggtgccgggt
     7801 gtcgggcttg ggttggcaat ttgtcgggcg attgtggatg tacacggcgg caccattacc
     7861 gcgttcaacc gaccggaagg tggtgcctgt tttcgtgtta cacttcccca gcaaaccgct
     7921 cctgaacttg aagactttca tgaggatatg tgacaaacgt tctgattgtt gaagatgaac
     7981 aggctattcg tcgctttctg cgcacggcgc tggagggcga cgggatgcgc gtctttgagg
     8041 ccgaaactct gcaacgcggc ttgctggaag cggcaacccg taagccagat ttgattattc
     8101 tcgatctcgg cctgcccgat ggtgatggga ttgagtttat ccgcgacctg cgccagtgga
     8161 gcccggtgcc ggtgattgtg ctttccgcac gcagcgaaga gagcgacaag atcgccgcgc
     8221 tggatgccgg agcggatgat tatctgagta agccgtttgg tattggcgaa ttgcaggccc
     8281 gtctgcgcgt cgctttacgc cgccactccg ccactccgcc accgccgcgc ccgatccgct
     8341 ggtgaaattt tccggtgtta ccgtcgattt agccgcccgc gtgattcacc ggggtgagga
     8401 agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca acaatgccgg
     8461 aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg cggtcgaaca
     8521 cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac aggatccggc
     8581 ccggcctcgc cacttcatta ctgaaaccgg tattgggtat cggtttatgc tttgaattgt
     8641 t
//
LOCUS       DAZPEU010000163         8528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPEU010000163 DAZPEU010000000
VERSION     DAZPEU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.164"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..189
                     /gene="csgC"
                     /locus_tag="ACHU2L_003261"
     CDS             <1..189
                     /gene="csgC"
                     /locus_tag="ACHU2L_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR1357998.1"
                     /translation="GSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWTPSSEKS"
     gene            310..621
                     /gene="ymdA"
                     /locus_tag="ACHU2L_003262"
     CDS             310..621
                     /gene="ymdA"
                     /locus_tag="ACHU2L_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR1357999.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            716..1249
                     /gene="ymdB"
                     /locus_tag="ACHU2L_003263"
     CDS             716..1249
                     /gene="ymdB"
                     /locus_tag="ACHU2L_003263"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR1358000.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1191..2672
                     /gene="clsC"
                     /locus_tag="ACHU2L_003264"
     CDS             1191..2672
                     /gene="clsC"
                     /locus_tag="ACHU2L_003264"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR1358001.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHU2L_003265"
     CDS             complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHU2L_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR1358002.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHU2L_003266"
     CDS             4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHU2L_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR1358003.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHU2L_003267"
     CDS             5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHU2L_003267"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR1358004.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2150 a         2025 c         2222 g         2131 t
ORIGIN      
        1 ggcagttcag ggcaaagtca gacgaagcaa gaaaagaccc tctcattgcc tgctaatcaa
       61 cccattgctt tgacgaagtt gagtttaaat atttccccgg aagatcgggt gaaaatagtt
      121 gttactgttt ctgatggaca ggcacttcat ttatcacaac aatggacgcc ctcttcagaa
      181 aagtcttaat ttgttgaaat atcgagcata agatgaatct ggagagaatg gtctgctgcg
      241 aatcagccaa cctgaaagta tggataacac aaccttcaag gatgactaat cattgaggaa
      301 atagaataaa tgttcagacc ttttttagac tctcttatgc tcggcagttt gttttttcct
      361 tttattgcca ttgcaggaag caccgcgcaa gggggcgtga tccattttta tggccaaatt
      421 gtggaaccgg catgtgacgt cagcacccag tcatcacccg tagaaatgaa ctgcccacag
      481 aatggttcta ttccgggcaa aacctactcc agcaaagcgt taatgagcgg caatgtcaaa
      541 aacgcgcaaa tagcctcagt aaaagtgcag tatcttgata aacagaaaaa gctggctgta
      601 atgaacatcg aatataacta agtttctgaa caactcacgt tgctgagcag aaaaatgcga
      661 tttaaccaaa aagcctgctg tacacttaag aaacaagaag gtgtaaggag gcgttatgaa
      721 aacgcgtatt catgttgtgc agggtgatat taccaaactg gccgttgatg tgattgtgaa
      781 tgcggctaat ccgtcattaa tgggaggcgg cggcgtcgat ggggccattc atcgcgcagc
      841 gggtccggcc ctgctggatg cctgtttaaa agtcaggcaa cagcagggcg attgccctac
      901 ggggcatgcc gttattacgc ttgcaggcga tcttcccgct aaagccgtag tacacaccgt
      961 cgggccagtc tggcgtggtg gggaacaaaa cgaagaccag cttttgcagg atgcctatct
     1021 gaatagccta cgactggtgg cggcaaacag ctatacgtca gtggcttttc ctgcaatcag
     1081 tactggggtt tatagttacc ctcgtgcggc agcggctgaa atcgcagtta aaaccgtttc
     1141 agaatttatt acccgtcacg ctttacccga acaggtatac tttgtctgtt atgatgaaga
     1201 aaacgcccac ctctacgaaa gactccttac ccaacaagga gatgaatgat ttgccccggc
     1261 tggcgagcgc ggtgctgcca ctgtgttcgc aacatcccgg tcagtgtggt cttttccctc
     1321 tggagaaaag tctggatgcg tttgccgccc ggtatcgtct ggccgaaatg gcagagcata
     1381 cgctggatgt tcagtattac atctggcagg acgatatgtc ggggcggtta ctgttttccg
     1441 ccctgttagc cgcagcaaag cgtggcgttc gcgtccgttt gttgctggac gacaacaata
     1501 cgcccggact tgacgacatt ttgcgcttgc ttgacagtca tccacgcatt gaagtccggc
     1561 tttttaatcc tttctcgttt cgcttgctac gtccgcttgg atatatcacc gacttttccc
     1621 gtctcaatcg ccggatgcac aataaaagtt tcactgtcga tggcgtggtt accctagtgg
     1681 gaggacgaaa tattggtgat gcctattttg gtgcagggga agagccactt ttttcggatt
     1741 tagatgtcat ggcaataggc cccgtggtag aggatgttgc cgatgatttc gcccgctact
     1801 ggtattgcaa atcggtttca cccttacagc aggtgctgga tgtcccggaa ggtgaaatgg
     1861 cggatcgcat cgagttaccc gcctcctggc ataacgatgc catgacgcat cgttatttac
     1921 gcaaaatgga atccagtcca tttataaatc atctggttga tggaacgttg ccgcttatct
     1981 gggcgaagac acgtttatta agtgatgatc cggcgaaagg ggagggcaag gcaaaacggc
     2041 attcactgtt accgcagcgc ctgttcgata tcatgggctc acccagtgaa cgcatcgata
     2101 ttatctcttc ctattttgta ccgacacgcg caggtgtggc gcaactctta cggatggtga
     2161 gaaaaggggt aaagattgcg atcctaacca attctcttgc cgctaacgat gttgctgtcg
     2221 tccatgccgg atacgcgcgc tggcgcaaaa aattgctccg ctatggcgtg gaattatatg
     2281 aactcaagcc gacgcgtgaa caaagcagta cgttacacga tcgcggcata accggtaatt
     2341 ccggtgccag cctgcatgct aaaactttta gcatcgatgg taaaacggtg tttatcggct
     2401 ctttcaattt cgatccgcgt tcaacattgc tcaatactga aatgggcttc gtgatagaga
     2461 gcgaaacgct ggcacagtta attgataaac gctttattca gagccagtac gatgcggcct
     2521 ggcagctccg tctggacagg tggggacgga tcaactgggt tgatcgtcat gcaaagaaag
     2581 aggttattct caaaaaagaa cccgccacca gtttctggaa gcgggttatg gtcagactgg
     2641 cgtcgatatt gcccgtggaa tggttattgt aaatgtggct taacgggctg gtgctttatc
     2701 gttctcacgc ttaacaaccg gtttaccaga gaacaaaaac ttcagtaacg ggatgcgtaa
     2761 atgaatttca tacagaatta tcgcaatccc tactacgaat atcaggccac agagaaaacc
     2821 aagccagttg gaggtgatgt gcggtgtaat gtatgcgccg aaaaacagcg ttaacgggtg
     2881 gtgaaccaga tagataaaca gcgatgcgtt aacaaaataa gtcacccgcg ctgactggaa
     2941 gttaagcaaa cggtggccaa aggagaagac cacattcacc atccacaggc cgaggaccat
     3001 ggtgatcacc gactcggttt cgtacatcca ggcatcgcca ctgccatagc gctggttgag
     3061 taaataagcg acaaacgcca atgctgcagc gagggtacag ccacgagacg gcgtggtaaa
     3121 caaggcttta agatgaggga aaatgaaagc cagtgcgccg aggataaaga acggtaaata
     3181 aaatagcgtt tgcatgacaa taaaattgaa catgccatta ctcagaatgg gtggatacac
     3241 aataaaaatc gttcttctta ttaccgcata gccgatgccg aggcataaaa aaatcaccga
     3301 tagttttacc atcgagaatt ttttattcgt tttatcagaa ttttctaaat tatttctgat
     3361 gcgcttaaat atccatacac acagcgtcgt catgactact aacaccagta aaaaccacag
     3421 gtgtgatatt aattcccagg ccaacgtatt atatttgtca tacaatgata gcccaggcca
     3481 actttccgct ttccctttga catattgcag cataataaat tgcggtaatg tcagtagggg
     3541 gatggctgtt aacatcggga tacctacacg ttcgacacgt actttccacc attttttcaa
     3601 gggatagcgt aaaaaaagca tgtaggaaaa gtatccggat ataacgaaaa atacctgcat
     3661 gcggaacgag tggatgaagt cattaaaaag ggttagccac aatgacggtt cggcgctatt
     3721 cacatgccat gtatggctcg aatagattaa agaaatatga aaagggatcc ctaacaacat
     3781 cagccaggcg cggatggagt cgaggaaata ttcacgttgc gcgggtactg gattcatata
     3841 tggttaacta atctcggatt tttcgtctta tccctgtcgg gttatgcctt taggcttgtt
     3901 gccatagcga caccgacctg accgcgccag gcgcaggctt caaggttttt atgcatagca
     3961 tcatcgctac cactaaccag aatggaagcg tctgtaagac ggttgataaa taaatttgct
     4021 ggcaaaccct acacgaagtc gatgcttctg tctttaggag aagcacggaa agtgaaaacg
     4081 gttgcaatca ggtgcttaat ccatgagcca gcgtgctgaa cgataccggg attcagttgt
     4141 cggaatggct ggttatccat taaaatagat cggatcgata taagcacaca aagggggaag
     4201 tgcttactaa ttatgaaaca taaactacaa atgatgaaaa tgcgttggtt gagtgctgca
     4261 gtaatgttaa ccctgtatac atcttcaagc tgggctttca gtattgatga tgtcgcaaag
     4321 caagctcaat ccttagccgg gaaaggctat gaggcgccta aaagcaactt gccctccgtt
     4381 ttccgcgaca tgaaatacgc ggactatcag cagatccagt ttaatcatga caaagcgtac
     4441 tggaacaatc tgaagacccc attcaaactc gagttctacc atcagggtat gtacttcgat
     4501 accccggtca aaataaatga agtgactgcc accgcagtca aacgaatcaa atacagcccg
     4561 gattatttca ctttcggcga tgttcagcat gacaaagaca cggtaaaaga ccttggtttt
     4621 gccggtttta aagtgcttta cccgatcaac agcaaagata aaaacgatga aatcgtcagc
     4681 atgctcgggg ccagctattt ccgcgtgatt ggtgcaggtc aggtttatgg cctttctgcc
     4741 cgcggcctag caattgatac cgctttgcca tcgggtgaag aatttccacg cttcaaagag
     4801 ttctggatcg agcgtccaaa accgactgat aaacgtttaa ccatttatgc attgcttgac
     4861 tcgccgcgtg cgacaggtgc ttacaaattc gtggttatgc cagggcgtga cacggttgtg
     4921 gatgtgcagt cgaaaatcta tctgcgcgat aaagtcggca aactgggggt tgcaccgtta
     4981 accagtatgt tcctgtttgg gccgaaccaa ccgtcgcctg caaataacta tcgtccggag
     5041 ttgcacgact ctaacggtct gtctatccat gctggtaatg gcgaatggat ctggcgtccg
     5101 ttgaataacc cgaaacattt agcggtcagc agcttctcca tggaaaaccc gcgaggcttt
     5161 ggtctgttgc aacgaggtcg tgatttctcc cgctttgaag atctcgatga tcgttacgat
     5221 ctccgtccaa gcgcatgggt gactccgaaa ggggagtggg gcaaaggcag cgttgagctg
     5281 gtggaaattc caaccaacga tgaaaccaac gataacatcg tcgcttactg gacgccggat
     5341 cagctgccgg agccgggtaa agagatgaac tttaaataca ccatcacctt cagccgtgat
     5401 gaagacaaac tgcatgcgcc agataacgca tgggtgcaac aaacgcgtcg ttcaacgggg
     5461 gatgtgaagc agtcgaacct gattcgccag cctgacggta ctatcgcctt tgtggtcgat
     5521 tttaccggcg cagagatgaa aaaactgcca gaggataccc cggtcacagc gcaaaccagc
     5581 attggtgata atggtgagat agttgaaagc acggtgcgct ataacccggt taccaaaggc
     5641 tggcgtctgg tgatgcgtgt gaaagtgaaa gatgccaaga aaatcactga aatgcgtgct
     5701 gcgctggtga atgccgatca gacgttgagt gaaacctgga gctaccagtt acctgccaat
     5761 gaataagaca actgagtaca ttgacgcaat gcccatcgcc gcaagcgaga aagcagcatt
     5821 gccgaagact gatatccgcg ccgttcatca ggcgctggat gccgaacacc gcacctgggc
     5881 gcgggaggat gattccccgc aaggctcggt aaaggcgcgt ctggaacaag cctggccaga
     5941 ttcacttgct gatggacagt taattaaaga cgacgaaggg cgcgatcagc ttaaggcgat
     6001 gccagaagca aaacgctcct cgatgtttcc cgacccgtgg cgtaccaacc cggtaggccg
     6061 tttctgggat cgcctgcgtg gacgcgatgt cacgccgcgc tatctggctc gtttgaccaa
     6121 agaagagcag gagagcgagc aaaagtggcg taccgttggt accatccgcc gttacattct
     6181 gttgatcctg acgctcgcgc aaactgttgt cgcgacctgg tatatgaaga ccattcttcc
     6241 ttatcagggt tgggcgctga ttaatcctat ggatatggtt ggtcaggatt tgtgggtttc
     6301 ctttatgcag cttctgcctt atatgctgca aaccggtatc ctgatccttt ttgcggtact
     6361 gttctgttgg gtgtccgccg gattctggac ggcgttgatg ggcttcctgc aactgcttat
     6421 tggtcgcgat aaatacagta tatctgcgtc aacagttggc gatgaaccat taaacccgga
     6481 gcatcgcacg gcgttgatca tgcctatctg taacgaagac gtgaaccgtg tttttgctgg
     6541 cttgcgtgca acgtgggaat cagtaaaagc caccgggaat gccaaacact ttgatgtcta
     6601 cattctgagt gacagttata acccggatat ctgcgtcgca gagcaaaaag cctggatgga
     6661 gcttatcgct gaagtcggtg gcgaaggtca gattttctat cgccgccgcc gtcgccgcgt
     6721 gaagcgtaaa agcggtaata tcgatgactt ctgccgtcgc tggggcagcc agtacagcta
     6781 catggtggtg ctggatgctg actcggtaat gaccggtgat tgtttatgcg gcctggtgcg
     6841 cttgatggaa gccaacccga acgccgggat cattcagtcg tcgccgaaag cgtccggtat
     6901 ggatacgctg tatgcgcgct gtcagcagtt cgcgacccgc gtgtatgggc cactgtttac
     6961 agccggtttg cacttctggc aacttggcga gtcgcactac tggggacata acgcgattat
     7021 ccgcgtgaaa ccatttatcg agcactgcgc actggctccg ctgccgggcg aaggttcctt
     7081 tgccggttca atcctgtcac atgacttcgt agaagcggcg ttgatgcgcc gtgcaggttg
     7141 gggggtctgg attgcttacg atctcccggg ttcttatgaa gaattgccgc ctaacttgct
     7201 tgatgagcta aaacgtgacc gccgctggtg ccacggtaac ctgatgaact tccgtctgtt
     7261 cctggttaag ggtatgcacc cggttcaccg tgcggtgttc ctgacgggcg tgatgtctta
     7321 tctctccgct ccgctgtggt ttatgttcct cgcgctctct actgcattgc aggtagtgca
     7381 tgcgttgacc gaaccgcaat acttcctgca accacggcag ttgttcccgg tgtggccgca
     7441 gtggcgtcct gagctggcga ttgcactttt tgcttcgacc atggtgctgt tgttcctgcc
     7501 gaagttattg agcattttgc ttatctggtg caaaggaacg aaagaatacg gcggcttctg
     7561 gcgcgttaca ttatcgttgc tgctggaagt gctgttttcc gtgctgctgg ctccggtacg
     7621 catgctgttc catacggtct tcgttgtcag cgcgttcctt ggctgggaag tggtgtggaa
     7681 ttcaccgcag cgtgatgatg actccacttc atggggcgaa gcgttcaaac gccacggctc
     7741 acagctgctg ttggggttag tgtgggctgt tgggatggcg tggctggatc tgcgtttcct
     7801 gttctggctg gcaccgattg tcttctcgtt gatcctgtca ccgtttgttt cggtgatttc
     7861 cagccgtgcc accgttggtc tgcgcaccaa acgctggaaa ctgttcctga tcccggaaga
     7921 gtattcaccg ccgcaggtgc tggttgatac cgatcggttc cttgagatga atcgccaacg
     7981 ctcccttgac gatggcttta tgcacgcagt gtttaacccg tcatttaacg ctctggcaac
     8041 cgcaatggcg acagcgcgtc accgcgccag taaggtgctg gaaatcgccc gtgaccgcca
     8101 cgttgaacag gcgcttaacg agacgccaga gaagctgaat cgcgatcgtc gcctggtgct
     8161 gctaagcgat ccggtgacga tggcccgtct gcatttccgc gtctggaatt cccctgaaag
     8221 atattcttca tgggtgagtt attacgaagg gataaagctc aatccactgg cattgcgtaa
     8281 accggatgcg gcttcgcaat aaaaacgtag ttgcctgatg cgctacgctt atcaggccta
     8341 catcgttcct gcaatttatt gattttgcaa gattttgtag gtcggataag gcgttcacgc
     8401 cacatccggc aaaaacaacg agccaataaa aataccggcg ttatgccgta atgccagtca
     8461 gttaagcaac tgactggctc tttttcgggg ctgtggggta tttccagggc ctctccttta
     8521 ccactctc
//
LOCUS       DAZPEU010000164         8485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPEU010000164 DAZPEU010000000
VERSION     DAZPEU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.165"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            44..397
                     /locus_tag="ACHU2L_003268"
     CDS             44..397
                     /locus_tag="ACHU2L_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR1358005.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHU2L_003269"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHU2L_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR1358006.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHU2L_003270"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHU2L_003270"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR1358007.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHU2L_003271"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHU2L_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR1358008.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHU2L_003272"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHU2L_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR1358009.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHU2L_003273"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHU2L_003273"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR1358010.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHU2L_003274"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHU2L_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1358011.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="ACHU2L_003275"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="ACHU2L_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR1358012.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="ACHU2L_003276"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="ACHU2L_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR1358013.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7609..8485
                     /locus_tag="ACHU2L_003277"
                     /pseudo
     CDS             7609..8485
                     /locus_tag="ACHU2L_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1900 a         2305 c         2285 g         1995 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctgaggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaa
//
LOCUS       DAZPEU010000165         8412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPEU010000165 DAZPEU010000000
VERSION     DAZPEU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.166"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..556
                     /locus_tag="ACHU2L_003278"
     CDS             <1..556
                     /locus_tag="ACHU2L_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFR1358014.1"
                     /translation="DDKFDNFMRQHRFSKVNFDTQQMIHFINRFNMNKGLVDKNNNFF
                     IQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNIF
                     ILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKS
                     HGKYALFPIFTASWRATRIKNKGI"
     gene            779..1276
                     /gene="ubiC"
                     /locus_tag="ACHU2L_003279"
     CDS             779..1276
                     /gene="ubiC"
                     /locus_tag="ACHU2L_003279"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR1358015.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1289..2161
                     /gene="ubiA"
                     /locus_tag="ACHU2L_003280"
     CDS             1289..2161
                     /gene="ubiA"
                     /locus_tag="ACHU2L_003280"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR1358016.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2316..4739)
                     /gene="plsB"
                     /locus_tag="ACHU2L_003281"
     CDS             complement(2316..4739)
                     /gene="plsB"
                     /locus_tag="ACHU2L_003281"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR1358017.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4910..5278
                     /gene="dgkA"
                     /locus_tag="ACHU2L_003282"
     CDS             4910..5278
                     /gene="dgkA"
                     /locus_tag="ACHU2L_003282"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR1358018.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5388..5996
                     /gene="lexA"
                     /locus_tag="ACHU2L_003283"
     CDS             5388..5996
                     /gene="lexA"
                     /locus_tag="ACHU2L_003283"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR1358019.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6069..7394
                     /gene="dinF"
                     /locus_tag="ACHU2L_003284"
     CDS             6069..7394
                     /gene="dinF"
                     /locus_tag="ACHU2L_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR1358020.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7510..7719
                     /locus_tag="ACHU2L_003285"
     CDS             7510..7719
                     /locus_tag="ACHU2L_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR1358021.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7761..8276)
                     /gene="zur"
                     /locus_tag="ACHU2L_003286"
     CDS             complement(7761..8276)
                     /gene="zur"
                     /locus_tag="ACHU2L_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR1358022.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1927 a         2029 c         2277 g         2179 t
ORIGIN      
        1 agatgacaag ttcgataatt ttatgcgtca acatcgattc agtaaggtga atttcgatac
       61 ccaacagatg atccatttta ttaacagatt taatatgaat aaagggctgg ttgataaaaa
      121 taacaatttt tttatccaac ttatcgatca ggccttacga tcaacggatg atatgatcaa
      181 agcaaatgcc tggtatcttt ataaagagtg gattcgtagt gatgatgttt cacctatatt
      241 tatagaaact gaagaaaagt taagaacctt taacactaat aaattaacac gaaacgataa
      301 tatatttatc ctgttctcct ctgtcgatga tgggcctgtt atggtggtaa gctcccagcg
      361 cttacatgat atgttgaatc ctacaaaaga taccaattgg aattccacgt gtatctataa
      421 atccagacat aagatgttgc ctattaatct tactcaggaa acacttttca gctccaaatc
      481 tcatggtaaa tatgcgcttt tcccaatttt tactgcgagt tggcgagcta ctcgtataaa
      541 gaataaaggt atttaagtaa aggaaaacat caccgttcct agcatcatgg gcggtgatgc
      601 ccctatggtt gccctcgcca gcacgggcat cggcaaagcg taaggttcaa cagcgtttta
      661 ccacttcatg cgattgttgc gtttttgttg cgcattagat cacttaattt gctttacatc
      721 tcccgtaaac acttttctgc gatacaatgc ctttacgtta tgtaacggag agttcggcat
      781 gtcacacccc gcgttaacgc aactgcgtgc gctgcgctat tttaaagaga tccctgccct
      841 ggatccgcaa ctgctcgact ggctgttgct ggaggattcc atgacaaaac gttttgaaca
      901 gcagggaaaa acggtgagtg tgacgatgat ccgcgagggg tttgtcgagc agaatgaaat
      961 ccccgaagaa ctgccgctgc tgccgaaaga gtctcgttac tggttacgtg aaattttgtt
     1021 atgtgccgat ggtgaaccgt ggcttgccgg tcgtaccgtc gttcctgtat caacattaag
     1081 cgggccggaa ctagcgttac aaaaattggg taaaacgccg ttaggacgct atctgttcac
     1141 atcatcgaca ctaacccggg attttatcga gataggccgt gatgccggac tgtgggggcg
     1201 acgttcccgc ctgcgattaa gcggtaaacc gctgttgcta acagaactgt ttttaccggc
     1261 gtcaccgttg tactaagagg aaaaaaatat ggagtggagt ctgacgcaga ataagctgct
     1321 ggcgtttcat cgcttaatgc gtacggataa gccaattggc gcgttactgc tgctctggcc
     1381 aacattatgg gcgttgtggg tggcgacacc gggcgttcct cagctctgga tcatggcggt
     1441 gtttgtcgcg ggtgtctggc tgatgcgcgc tgctggttgc gtggtgaatg attatgctga
     1501 tcgcaagttt gatggtcatg ttaagcgcac ggcgaaccga ccacttccca gcggcgcggt
     1561 aacagagaaa gaggcgcgcg cgctgtttgt agtgctggta ctgatttcgt ttttactggt
     1621 tctgacgctg aatacgatga ccattctgtt gtcgattgcc gctctggcat tggcgtgggt
     1681 gtacccgttt atgaagcggt atacccatct cccgcaagtg gtgctgggcg cggcgtttgg
     1741 ctggtcgatt ccaatggctt ttgccgctgt gagtgagtcg gtgccattga gttgctggtt
     1801 aatgttcctc gccaatattc tctgggcggt ggcttacgac acgcagtatg cgatggttga
     1861 ccgcgatgat gatgtgaaga ttggcattaa atccacggca atcctgttcg gccaatacga
     1921 taaattgatt attggtattt tgcagattgg cgtactggca ctgatggcga tcatcggtga
     1981 gttaaatggc ttaggctggg gatattactg gtcaattgtg gtggctggcg cgctgtttgt
     2041 ttatcaacaa aaactgattg ccaaccgcga gcgtgaagcc tgctttaaag catttatgaa
     2101 taataactat gttggtctgg tactgttttt aggactggca atgagttact ggcatttctg
     2161 atgatgtaaa aaagccggat gatcatccgg ctttcttctg ggttgcctga tgcgttgcgc
     2221 ttctcaggcc tacccggtga atcgcaattt attgaatttg caggtcatgg taggccggat
     2281 aaggcgtttt cgccgcatcc ggcaattctc tctgattacc cttcgccctg cgtcgcactc
     2341 tcaatcgtca aacgcacgtc tgatgtaatc aactccgcca gcaactgata aaccttcatc
     2401 gtttctgctg gttcggcatc gccgctatcg ctgatatacc cttcatcacg cagcgtcagc
     2461 accagagaac tgaacaccgc cttgtcgaag aactccggcg cgttgatgcc gtgcagcacg
     2521 gagagacgtt gcgcaacggt gcggctctct ttctccagcg taccgcggtt gatcgacggg
     2581 ttggcactca acaaccagaa ggtgatggca taacgttgca gcgtttcgcg cgcgcctgcg
     2641 gccagcagct gtagggtgcg agaatgcgcc gggttgatat gcaactcatc atcttgcagg
     2701 gtaatcagcc cctgacgttg catctcattt gccagcgcat caataacgtc cggcaactcg
     2761 tcgcgatccc agcgcaggaa cagctccgct ttcagcattg ggtaaagcac attgacgtgc
     2821 tccatcaata cgtcgcggga gatgtggcga tgctgggtga caattgccgc catcagcgaa
     2881 ggcagcacca acatatgcgc aatgttgttg cgatagtagg tcatcagcac cgcttgctcg
     2941 cgtggcagaa tgatgatgtc gccgatagtg tctttctcga cttcaaactt gttcatttgc
     3001 agcgcgtgat cgataagctc gctggcgctg gctgaaggaa cggtagagtc cgtggagtaa
     3061 ggtacgttgc gcatcagatc caggtagcag ttgagttgct cggttaactg ctcgcgggtg
     3121 agtgagcgct gacgtgatgc cagtagcgca gtacagcaca ggttcatggc gtttgccgcg
     3181 cctgcgttgt taatgcgtac catcagatcg gcagcaatat tattgaccgt cggcgttaac
     3241 caggccggac gcaccgcttc gatgggatcg atagattcac gccagtctgg tacgtgctgg
     3301 ttaaggtagg tcatcaacgg cattggttca ccgaagttga cgtaaccctg accgagatta
     3361 cgcagcttgc ttaaaccgcg cagcatctgc ggcaggctct ctttctcttt cgtcgcgccg
     3421 cgcagttctt tggcgtaagt acccacttcc atgacgtgct cataaccgat atagatcgga
     3481 atcagcgtaa tcggacgcgt gccgccacgc agcatcgcct gaatggtcat cgacagcgta
     3541 ccggttttcg gatccagcaa acgccccgtg cgggaacgac cgccttccac gaagtactcg
     3601 acggaataac cacggctgaa cagttcgccg agatactcac ggaaaacggt ggaataaagt
     3661 ttattgcctt taaaagtacg gcgaataaag aacgccccca gacggcggaa aatcggcccg
     3721 gccggccaga agttcaggtt gatcccggcg gcgatatgcg gcggcaccag tccctggtgg
     3781 tacagcacgt aagaaagtag caggtagtcc atgtgactgc ggtggcaagg cacatacacc
     3841 agctcatgac cgtcgtgggc cagctggcga acgcgctcgg cgttatggac gttgatgccc
     3901 tgataaaatc ggttccaggt gaagcccaga atgcggtcag tcaggcggat catctcgtaa
     3961 gagaaattcg ccgcaatctc ttccatcagc gcaatcgcgt tctgctgcgc tttttcatgg
     4021 gagatttttt tgctgcgcgc ttcatcttct accgctttgg caatggcgcg ggaggcgagc
     4081 agcttattaa acagatcctg acgagcaggc agacgtgggc ctacggcagc cagacgttga
     4141 cgggcaaagt gcatacgcgc cacgcgcgcc agtttctgag cgatagtttt atccgtgccg
     4201 tgttcatccg ccatacggcg cagcgacact gacggcgaga aacgcacaaa actgtcgcga
     4261 ccgagccaca gtacagcgaa aaatttctgt acgccgttaa gcatacgcag cggcgggttc
     4321 acttcgcctt tttcacgccc cggcgcgcga ccaaacatca ccgatactgg caccatctgc
     4381 acatccagat ttgggttgct acggtgcaaa tcgagatagt cgtggaaaag cttaatagac
     4441 tcttctttcg gcgtgtaata ggtgaataca cgcggcccgc cgtgaatgaa cacatagcgc
     4501 ggcagtagcg tgccgtcgat ttccagcggc tctaacgggt caggcaaatc atgtgccaga
     4561 cactgggcgc gcaacgtcag caaatctgct ttcgagttgt acggtaaaac gtacataatt
     4621 ggacgagagg tatccagccc cagttccggg gcaggatctg ccggaataga cttgcttttt
     4681 accaggatgc ttaatggtaa attcagtaat ttgtagtaaa ttcgtggcca gccggacata
     4741 aacgatgtaa agcctctggt taataatgca aatgcgcggc aaggatagca gaaagtcatg
     4801 ggaaattctg tggtatccgc tcatgtttcg cgcggcgtta cgcaaacccg aatcatcgga
     4861 tttaacggta cactgatatt gacgctcata atgtaaaaag gttctttcaa tggccaataa
     4921 tacaactgga ttcacccgaa ttatcaaagc tgctggctat tcctggaaag gtttacgcgc
     4981 tgcatggatc aacgaagcgg cattccgtca ggaaggcgta gcggtattgt tggcggtggt
     5041 catcgcctgc tggctggatg tggacgcaat tacccgcgtg ctgcttatca gctccgtgat
     5101 gctggtgatg attgtggaaa tcctcaatag cgccatcgaa gcagtggttg accgaattgg
     5161 ctctgaatac catgagcttt ccggacgcgc aaaagatatg ggatccgccg cggtgctgat
     5221 tgccattatc gtcgccgtga ttacctggtg catcctgtta tggtcgcatt ttggataacc
     5281 cttccagatt tcgataaatc tctggtttat tgtgcagttt atggttccaa aatcgccttt
     5341 tgctgtatat actcacagta taactgtata tacacccagg gggcggaatg aaagcgttaa
     5401 cggccaggca acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc
     5461 cgccgacgcg tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag
     5521 aacatctgaa ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg
     5581 ggattcgtct gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg
     5641 gtgaaccgct tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttgt
     5701 tcaagccgaa tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca
     5761 ttatggatgg cgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg
     5821 ttgtcgcacg tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag
     5881 tcgaactgtt gccagaaaat agcgagttta aaccaattgt cgttgacctt cgtcagcaga
     5941 gcttcaccat tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctgtaacata
     6001 tctctgagac cgcgatgccg cttggcgtcg cggtttgttt ttcatctctc ttcatcaggc
     6061 ttgtctgcat ggcattcctc acttcatctg ataaagcact ctggcatctc gccttaccca
     6121 tgattttctc caatatcacc gttccgttgc tgggactggt cgatacggcg gtaattggtc
     6181 atcttgatag tccggtttat ttgggcggcg tggcggttgg tgcaacggcg accagctttc
     6241 tctttatgct gttgctgttt ttacgcatga gcaccaccgg gctgactgcg caggcttatg
     6301 gtgccaaaaa tcctcaggca ttagcccgtg cgctggtgca accgttgctg ttggcgttgg
     6361 gggccggggc gttaattgcg ctgttgcgta cgccgattat cgatctggcg ctgcatattg
     6421 ttggcggtag tgaagcggtc ctggaacagg cgcggcgctt tcttgaaatc cgctggttaa
     6481 gcgctccggc gtcgctggcg aatctggtat tactcggttg gttactcggc gtgcaatatg
     6541 cccgtgcgcc agtaattttg ttagtggtcg gcaatatcct caacattgtg ctggatgtct
     6601 ggctggtgat ggggctgcat atgaacgtgc agggcgcggc gctggcgacg gttattgcgg
     6661 aatatgcaac attgctgatt ggtctgctaa tggtgcgtaa aatcctcaaa ctacgcggaa
     6721 tttccggcga aatgctgaaa actgcctggc gaggaaactt ccgtcgcttg ctggcgctta
     6781 accgcgatat catgctgcgt tcgctgttgt tgcaactctg tttcggcgcg atcaccgtac
     6841 ttggtgcgcg actggggagt gacattatcg ctgttaacgc ggttctgatg acgctactca
     6901 cctttaccgc ctatgcgctt gatggttttg cctacgcggt tgaagcgcac tccggtcagg
     6961 catacggtgc gcgcgacggt agccagttgc tggatgtatg gcgggcagcg tgccgccagt
     7021 cggggattgt agcgttacta ttttcggtgg tttatttgct ggcaggggaa cacatcattg
     7081 cgttgctgac gtcgttaacc cagattcagc agctggctga ccgctatctt atctggcagg
     7141 tgattttgcc gttggttggc gtctggtgtt atctgctgga cggcatgttt ataggcgcaa
     7201 cgcgtgccgc cgaaatgcgt aacagtatgg cggtggccgc cgcaggtttt gcgctgacgc
     7261 tccttacgct gccgtggctg ggtaatcatg gtttgtggct ggcattaacc gtctttctgg
     7321 cgttgcgcgg gctttctctg gcggctatct ggcggcgtca ctggcgcaac ggtacctggt
     7381 ttgccgcaac gtgacggtta aaaattctga ataaattatc ctaagccaaa ttgctgacta
     7441 cacttaatct cacgttcaga agaaaagtga acgtactctc attcacaacc taacgatgag
     7501 gtcttgatta tgaataaaga tgaagccggc ggtaactgga aacagtttaa aggtaaagtg
     7561 aaagagcaat ggggcaaact gaccgatgat gatatgacga tcattgaagg taaacgtgat
     7621 caactggtcg gtaaaatcca ggaacgttat ggttatcaga aagatcaggc agaaaaagag
     7681 gtcgtggatt gggaaacccg caatgaatat cgctggtaat taatccctcc tgcccgacgt
     7741 gtacaaggat gtacgccctc ttaacgcggt ttctttttca cctgcacaga gtgatcatgc
     7801 tggcactgtt caggatgacg acacgcttcc acttccacgc atgccgcaca gagcccatgt
     7861 gcttcaatca cattatgccg cagggcaaac cccatttttg ccgccagcgt atgcataatg
     7921 tcttccacgc cttctgcaca ctcttctttc accgcgccgc agcgatcgca aataaacatg
     7981 gctgacgtat gcgtgggctg gtcgaacaga tgacagagca cataactgtt ggtggattcc
     8041 accttatgca caaaaccttg ctcaagcaga aaatccagcg cgcgataaac cgttggcggc
     8101 ttggcttgcg gttcagcttc gcgcagtaaa tcaagcagat cataagcgct gatagcgcca
     8161 tcctgcagac tcatcaggcg caacacttcc aggcgctgtg gggtcaggcg cacattacgc
     8221 tgcgcgcaga ttttttcagc ctgcgctaat aactcctgcg ttgtggtctt ttccatcggg
     8281 cacctcaaag tgggggttaa gaatgtcctt actttaccat gttccaggaa aaacaccgag
     8341 aacccttatt gttgccgtaa tgttgatttt ctgttttgta ggtaaggtgt tgtgttgcct
     8401 tatcgtacca tt
//
LOCUS       DAZPEU010000166         8399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPEU010000166 DAZPEU010000000
VERSION     DAZPEU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.167"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            317..1249
                     /gene="glaH"
                     /locus_tag="ACHU2L_003287"
     CDS             317..1249
                     /gene="glaH"
                     /locus_tag="ACHU2L_003287"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR1358023.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1269..2537
                     /gene="lhgO"
                     /locus_tag="ACHU2L_003288"
     CDS             1269..2537
                     /gene="lhgO"
                     /locus_tag="ACHU2L_003288"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR1358024.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2560..4008
                     /gene="gabD"
                     /locus_tag="ACHU2L_003289"
                     /pseudo
     CDS             2560..4008
                     /gene="gabD"
                     /locus_tag="ACHU2L_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            4016..5302
                     /gene="gabT"
                     /locus_tag="ACHU2L_003290"
                     /pseudo
     CDS             4016..5302
                     /gene="gabT"
                     /locus_tag="ACHU2L_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5540..6940
                     /gene="gabP"
                     /locus_tag="ACHU2L_003291"
     CDS             5540..6940
                     /gene="gabP"
                     /locus_tag="ACHU2L_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR1358025.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6961..7623
                     /gene="csiR"
                     /locus_tag="ACHU2L_003292"
     CDS             6961..7623
                     /gene="csiR"
                     /locus_tag="ACHU2L_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR1358026.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7624..8073)
                     /gene="kbp"
                     /locus_tag="ACHU2L_003293"
     CDS             complement(7624..8073)
                     /gene="kbp"
                     /locus_tag="ACHU2L_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR1358027.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8157..8315)
                     /locus_tag="ACHU2L_003294"
     CDS             complement(8157..8315)
                     /locus_tag="ACHU2L_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR1358028.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1903 a         2201 c         2332 g         1963 t
ORIGIN      
        1 cgataaaacg gggcagataa tatgtttagt ttactaacgg tcattttgta gtgaagccat
       61 ttactgtttt ttatcgacca gataatctgt tctctaatgt taactccccc taacctgttg
      121 ctttagttat tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat
      181 ttgttgcttt tgatcacaat aagaaagcaa tatgtcgctt ttgtgcgcat ttttcagaaa
      241 tgtagatatt tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat
      301 aagaggatcg cttctgatga atgcactgac cgccgtacaa aataacgctg tcgattcagg
      361 ccaggactat agcggattca ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact
      421 caccttcacc gaacagacga ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca
      481 ggcgctggag tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg
      541 tgcgaatcag ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct
      601 gttgatcaat gcggtaggta tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc
      661 aacggcggta gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta
      721 cgcgcgtttc gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc agccgcaccg
      781 cgtgatggaa ctgcacaacg acggcacgta tgtcgaagag atcaccgatt acgtgctgat
      841 gatgaaaatc gatgactggg aacatctgga ccactatttc cgccatccac tggcgcgtcg
      901 cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt tccatccagt
      961 gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat atcgaccagt tcgtccagcc
     1021 aaaagatttt gaagaaggcg tgtggttgag cgagctttcc gatgccattg aaaccagcaa
     1081 aggcattctt tctgtacccg ttcccgttgg caaattcctg ttgataaaca acctgttctg
     1141 gctgcacggt cgcgaccgct ttactccgca cccggatctg cgccgtgaac tgatgcgtca
     1201 gcgtggctat ttcgcttacg ccactcacca ctaccagacg catcagtaag cgcaaaggaa
     1261 ttgagcggat gtatgatttt gtgattattg gcggcggcat catcggcatg tcgaccgcca
     1321 tgcaactgat tgatgtttat ccggatgccc gcattgcgtt gctggaaaaa gagtccggcc
     1381 cggcctgtca ccagacgggc cacaacagcg gcgtgatcca tgccggggtc tattacacgc
     1441 ccggcagcct gaaggcgcag ttttgcctgg cggggaaccg tgccaccaaa gccttttgcg
     1501 atcaaaacgg cattcgctat gacaactgcg gcaagatgct ggtcgccacg tctcagctcg
     1561 aaatggaacg gatgcgcgcc ttatgggaac gcacagcggc gaacggtatc gagcgcgagt
     1621 ggataaacgc cgtggaactg cgcgagcgcg aaccgaatat caccgggctt ggtggcattt
     1681 ttgtgccgtc cagcggcatt gtcagctacc gcgaagtgac ggcggcaatg gcaaaaatct
     1741 tccaggccag aggcggcgag attatttata acgccgaagt cagcgccctc agtgagcata
     1801 aaaacggcgt ggtgatacgc acccgtcagg gcggtgaata tgaagcatcg acgctgatta
     1861 gctgttccgg gctgatggct gaccggctgg tgaaaatgct cggcctcgaa ccgggcttta
     1921 ttatctgccc gttccgtggc gagtatttcc gccttgcgcc ggagcataac cagattgtta
     1981 accacctgat ttaccccatt cccgaccccg cgatgccatt tttgggcgtg catctcaccc
     2041 gcatgatcga cggcagcgtg accgtcgggc caaacgcggt gctggctttc aaacgcgaag
     2101 gctatcgcaa gcgcgacttc tcatttagtg acacgctgga gattttgagc tcgtcgggga
     2161 ttcgccgggt gctgcaaaac catctacgct caggactggg cgagatgaaa aactcgctgt
     2221 gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg ttaagcgatc
     2281 tccagccctg gcccgccggt gtgcgggcgc aggcagtatc gccggacggc aagctgattg
     2341 acgattttct gtttgtcacc accccacgca cgatccacac ctgcaatgcg ccctccccgg
     2401 cagcgacatc agcaattcct attggtgcgc atattgtcag caaggtacaa acgctgttgg
     2461 caagccagag taaccccgga cgcacgctgc gagcggcacg tagtgtggat gccttacacg
     2521 ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa cgacagtaac
     2581 ttattccgcc agcaggcgtt gattaacggg gaatggctgg acgccaacaa tggtgaagtc
     2641 atcgacgtca ccaatccggc gaacggcgac aagctgggta gcgtgccgaa aatgggcgca
     2701 gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc tgcccgcctg gcgtgcgctc
     2761 accgccaaag aacgcgccac cattctgcgc aactggttca atttgatgat gcagcatcag
     2821 gacgatttag cgcgcctgat gaccctcgaa cagggtaaac cgctggctga agcgaaaggc
     2881 gaaatcagct acgccgcctc cttcattgag tggtttgctg aagaaggcaa acgtatttat
     2941 ggcgacacca ttcctggtca tcaggccgat aagcgcctga ttgttatcaa gcagccgatt
     3001 ggcgtcaccg ccgccatcac gccgtggaac tttccggcgg cgatgattac ccgtaaagcc
     3061 ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca gacgccgttc
     3121 tctgcgctgg cgctggcgga actggcgatt cgcgcgggca ttccggctgg cgtgtttaac
     3181 gtggtcaccg gttcggcagg cgtggtcggt aatgaactga ccagcaaccc gctggtacgc
     3241 aaactgtcgt ttaccggtta gaccgagatt ggccgccagt taatggaaca gtgcgcgaaa
     3301 gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt ctttgacgat
     3361 gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga aattccgcaa cgccgggcaa
     3421 acctgcgtct gcgccaaccg tttatacgtg caggacggcg tgtatgaccg ctttgccgaa
     3481 aaattacagc aggcggggag caaactgcac atcggcgacg ggctggagaa aggcgtcacc
     3541 atcgggccgc tgatcgatga aaaagcggta gcaaaagtgg aagagcatat tgccgatgcg
     3601 ctggagaaag gtgcgcgtgt ggtttgcggc ggtaaagcgc acgaacgcgg cggcaacttc
     3661 ttccagccaa ccattctggt ggacgttccg gccaacgcca aagtgtcgaa agaagagacg
     3721 ttcggccccc tcgccccact gttccgtttt aaagatgaag ccgatgtgat tgcacaagcc
     3781 aatgacaccg agtttggtct tgccgcctat ttctacgccc gtgatttaag ccgcgtcttc
     3841 cgcgtcggcg aggcgctgga gtacggcatc gtcggcatca ataccggcat tatttccaat
     3901 gaagtggccc cattcggcgg catcaaagcc tcgggtctgg gtcgtgaagg ttcgaagtat
     3961 ggcatcgaag attacttaga aatcaaatat atgtgcatcg gtctttaact ggagaatgcg
     4021 aaggaacagc aataaagagt taatgcagcg ccgcagtcag gcgattcccc gtggcgttgg
     4081 gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc cgggtgtggg acgtttaagg
     4141 ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc aataccgggc acctgcatcc
     4201 gaaggtggtg gccgcggtgg aagcgcagtt gaaaaaactg tcgcacacct gcttccaggt
     4261 gctggcttac gagccgtatc tggagctgtg cgagattatg aatcagaagg tgccgggcga
     4321 tttcgccaag aaaacgctgc tggttacgac cggttccgaa gcggtggaaa acgcggtaaa
     4381 aatcgcccgc gccgccacca aacgtagcgg caccatcgct tttagcggcg agtatcacgg
     4441 gcgcacgcat tacacgctgg cgctgaccgg caaggtgaat ccgtactctg cgggcatggg
     4501 gctgatgccg ggtcatgttt atcgcgcgct ttatccttgc ccgctgcacg gcataagcga
     4561 ggatgacgct atcgccagca tccaccggat cttcaaaaat gatgccgcgc cggaagatat
     4621 cgccgccatc atgattgagc cggttcaggg cgaaggcggt ttctacgccg cgtcgccagc
     4681 ctttatgcag cgtttacgcg ctctgtgtga cgagcacggg atcatgctga ttgccgatga
     4741 agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg atggagcaga tgggcgtggc
     4801 gccggatctt accacctttg cgaaatcgat cgcaggcggt ttcccgctgg cgggcgtcac
     4861 cgggcgcgcg gaagtgatgg acgctgttgc accgggcggt ctgggcggca cctatgccgg
     4921 taacccgatt gcctgcgtgg ctgcgctgga agtgttgaag gtgttcgagc aggaaaatct
     4981 gctgcaaaaa gccaacgatc tggggcagaa gctaaaagac ggattgttgg cgatagccga
     5041 aaaacacccg gagatcggcg acgtacgcgg gctgggatcg atgattgcca tcgagctgtt
     5101 tgaagatggc gatcacagca aaccggacgc aaaactcacc gccgagatcg tggcacgcgc
     5161 ccgcgataag ggcctgattc ttctctcctg cggcccgtat tacaacgtgc tgcgcatcct
     5221 tgtaccactc accattgaag acgctcagat ccgtcagggt ctggagatta tcggtcagtg
     5281 ttttgatgag gcgaagcagt agcaccgtcc ccatgcagga ggcgacgctg cgcgtcttgt
     5341 ccggcctacg agaatccaag tcggataagg cgtttacgcc gcatccgaca atctgtacgt
     5401 gaacaggaat aaatctatgt cggccggata aggcggagcc gctctccggc aaaaagaatc
     5461 aataacaatt aaacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac
     5521 actttcgaga ggattcagga tggggcaatc atcgcaacca cacgagttag gcggcgggct
     5581 gaaatcacgc cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt
     5641 cggttccagc gtcgccatcg ccgaagcgga cccggcggta ttactggcct atctgttcgc
     5701 cggattactg gtggttatga ttatgcggat gctggcggaa atggcagttg ccacgcccga
     5761 taccggttcg ttttccacct atgccgataa agccattggc cgctgggcgg gctataccat
     5821 cggctggttg tactggtggt tttgggtact ggttatccca ctggaagcca acatcgccgc
     5881 catgatcctg cactcgtggg ttccaggcat tcccatctgg ttattttccc tcgtcattac
     5941 cctcgcctta actggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg
     6001 gctggcgctg tgcaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat
     6061 tagcggtttt tacccttatg ccgaagtgag cgggatctca agattgtggg atagcggcgg
     6121 ctttatgccc aacggtttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt
     6181 tatgggcgca gaaattgtca ccattgccgc cgcggaatcc gacatgccgg aaaaacatat
     6241 tgtccgcgcc accaactcgg ttatctggcg tatttctatc ttctatttgt gctctatttt
     6301 tgtcgtcgtg gcattaatac cgtggaatat gccggggctg aaagccgttg gatcttatcg
     6361 ctcggttctg gaattgctca atattcccca tgcgaaatta atcatggact gtgtgatatt
     6421 actttccgta accagttgcc tgaactcggc cctgtatacc gcctcaagga tgctctactc
     6481 cttaagccgt cgcggtgatg ctcccgcggt aatgggtaaa atcaaccgca gtaaaacccc
     6541 gtacgtggcg gtgttactct ccaccggcgc agcattctta acggtggtgg tgaactatta
     6601 cgcgcctgcg aaggtattta aatttctgat cgacagctcc ggcgctatcg ccctgctggt
     6661 ttatttagtc atcgccgttt cacagttgcg gatgcgcaaa attctgcgag cagaaggaag
     6721 cgaaattcgc ttgcgaatgt ggcttgatcc ctggctcacc tggctggtca tcggctttat
     6781 tacctttgtg ttggtagtga tgctattccg tccggcgcaa cagttagaag tgatctccac
     6841 cggcttatta gcgataggga ttatctgtac cgtgccaatt atggctcgct ggaaaaagct
     6901 ggtattgtgg caaaaaacac ccgttcataa tacgcgctga taatgatcag gagtcacacc
     6961 atgaccatta cgtctctgga tggctatcgc tggctgaaga acgatattat tcgcggtaat
     7021 tttcaaccgg atgaaaaatt acggatgagt ttactgacat cgcgttatgc acttggcgtt
     7081 gggccgttgc aggaagcact ttctcaactg gtggcggaac ggctggtcac ggtggtgaat
     7141 caaaaagggt atcgggtggc gtctatgtca gagcaggagc tgctcgatat tttcgacgcc
     7201 cgcgccaata tggaagcgat gctggtcagt ctggcgattg cccgcggtgg cgatgaatgg
     7261 gaggcagacg ttctcgcaaa agcgcatctg ctcagtaagc tggaagcctg tgacgccagc
     7321 gagaaaatgc ttgatgagtg ggatctgcgt catcaggcgt ttcatacagc aattgtggcg
     7381 ggctgtggtt ctcactattt gctgcaaatg cgtgaacggt tgtttgatct ggcggcgcgt
     7441 tatcgattta tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga ggataaacac
     7501 gatcagcacc agaccctgac tgcggcggta ctgccgcgag atacagcgcg cgccagtgag
     7561 ttaatgcgcc agcatttact gacgccaatt cccattatcc agcaggcgat ggctggcaat
     7621 taactactct tccggaatac gcaacacttg ccccggataa attttatccg ggctttttag
     7681 catcggttta ttcgcttcga agattttatt gtacagatta gcgttaccgt agacctgttt
     7741 ggaaatggca ctcagagtgt cgccagactt aacggtataa aactggctgg cagtggctgg
     7801 tgtcgccgtt ttcacctgat catcaacact ggcaataccg gaaatattcc ccaccgcaac
     7861 aaggattttc tccttcgcct cctgactcag gccgtcacca gtgaccgtcg ctttgccgtc
     7921 ggcaatttga atgttcactt tatcggcatc aggtataccg gttttgctca gatgctcctg
     7981 caccttcttc gcctgatcgt ctttatcgtg ctgacctgta accgcatccc agagtttttc
     8041 tccggcatct ttaacaaaat tgaacagacc catagctacc tcattcgtta acggaaacac
     8101 tcagaaagtg tagcagagga acgcggcgcg ttaacgccgc gggggtgagc acaggtttaa
     8161 tcgcgggttt gcacccagaa cgcgtgaatc aaaccgggga tatacccaag cagtgtcagc
     8221 agaatattaa taatgaacgc ccaaccgaac cctttaccga gcagcacgcc gatcggcggc
     8281 agaatgatgg tgatgacgat tctccagaaa cccatatgta ctccctataa gaaaattact
     8341 cattgtttaa aaagagattt tatctcttaa gcgtagtaat tttagcggag cctgccagt
//
LOCUS       DAZPEU010000167         8376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPEU010000167 DAZPEU010000000
VERSION     DAZPEU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.168"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..958
                     /gene="espL1"
                     /locus_tag="ACHU2L_003295"
                     /pseudo
     CDS             1..958
                     /gene="espL1"
                     /locus_tag="ACHU2L_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1130..1237
                     /gene="yniD"
                     /locus_tag="ACHU2L_003296"
     CDS             1130..1237
                     /gene="yniD"
                     /locus_tag="ACHU2L_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR1358029.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1290..2048)
                     /locus_tag="ACHU2L_003297"
     CDS             complement(1290..2048)
                     /locus_tag="ACHU2L_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR1358030.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2335..3264
                     /gene="pfkB"
                     /locus_tag="ACHU2L_003298"
     CDS             2335..3264
                     /gene="pfkB"
                     /locus_tag="ACHU2L_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR1358031.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3365..3655
                     /gene="ghoS"
                     /locus_tag="ACHU2L_003299"
     CDS             3365..3655
                     /gene="ghoS"
                     /locus_tag="ACHU2L_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1358032.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3761..4621
                     /locus_tag="ACHU2L_003300"
     CDS             3761..4621
                     /locus_tag="ACHU2L_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR1358033.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4662..5198)
                     /locus_tag="ACHU2L_003301"
     CDS             complement(4662..5198)
                     /locus_tag="ACHU2L_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR1358034.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5345..6013
                     /gene="hxpB"
                     /locus_tag="ACHU2L_003302"
     CDS             5345..6013
                     /gene="hxpB"
                     /locus_tag="ACHU2L_003302"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR1358035.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6176..6766
                     /locus_tag="ACHU2L_003303"
     CDS             6176..6766
                     /locus_tag="ACHU2L_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1358036.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6899..8289
                     /gene="tcyP"
                     /locus_tag="ACHU2L_003304"
                     /pseudo
     CDS             6899..8289
                     /gene="tcyP"
                     /locus_tag="ACHU2L_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8293..>8376)
                     /locus_tag="ACHU2L_003305"
     CDS             complement(8293..>8376)
                     /locus_tag="ACHU2L_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358037.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         2173 a         2004 c         2009 g         2190 t
ORIGIN      
        1 gatgctcaga cctgggctga taggtatgtt cgcaactgtg caattgctat tctgccccta
       61 ttacctgtgg gagttctagg ggctattttc gcgggtattg catcacgaat gccatttgcc
      121 cctatacatc catcggcaat gttgttaata atggctacag gccagactca acagcttatt
      181 acattattca aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg
      241 cagaatagcg ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac
      301 gtgaaaatat ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat
      361 aatctggtta aattactgca aactaaaagt gctaacgaaa cacctggact ttatatctcc
      421 atgttgtatg gattcgatga aataatcgat atctttctga atacattagc cactccaata
      481 gcactaagag cttttaaaca aaaaactggt gatgagtatt ttagccatga aaatacatga
      541 tggtgagcca ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt
      601 cctctctaaa attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga
      661 tttattaaaa ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg
      721 taatgaagac gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc
      781 ttttagtcaa catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc
      841 tgttcatata gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat
      901 aaatgtcatc agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc
      961 agctatttac gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt
     1021 gaccatgtgc acaacgccac ccgacggctg taataacaaa aaatcccttg atgcctgtcc
     1081 cttttgttac actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa
     1141 acgctttgat aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat
     1201 gttgttgcta cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca
     1261 gaccgcataa cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc
     1321 cgacggtcgg tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa
     1381 gccaccttca gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc
     1441 gttgtgtctt ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac
     1501 tgccatgcat aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta
     1561 tatttgtcgt aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc
     1621 aaaaactgac gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg
     1681 tctgtcagcc agcttgcctg accaaataaa tagtcataat cagttaaatt aaagcggcta
     1741 cgcccgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
     1801 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
     1861 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
     1921 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
     1981 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
     2041 agcttcattt atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt
     2101 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
     2161 acttagcaat gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta
     2221 tttatatata tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt
     2281 cttcactttc cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta
     2341 cgtatctata cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt
     2401 tatcccgaag gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc
     2461 aacgtcgccc gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt
     2521 ggcgcgaccg gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact
     2581 gtagaagcca aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa
     2641 cagtatcgtt ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa
     2701 gagcaagttc tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca
     2761 ggtgtgaagc tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc
     2821 tgcatcatcg acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag
     2881 ttggttaagc ctaaccaaaa agaactcagt gcgctggtga atcgcgaact cacccagcca
     2941 gacgatgtcc gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc
     3001 gtttcccttg gtccacaagg agcgctgggt gtcgatagtg aaaactgcat tcaggtggtg
     3061 ccaccaccgg tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg
     3121 acactgaaac tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg
     3181 gggagtgcag ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa
     3241 atttacgctt acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc
     3301 aacctgtcgg caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg
     3361 aactatggcc agtggcgatc ttgtccatta tgtcataacc gtcatgttgc atgaggatac
     3421 attgactgaa attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat
     3481 gacggatgat gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac
     3541 ccaaagtgaa gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa
     3601 agatcctgaa atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt
     3661 ttttgggcaa taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc
     3721 gctaacctta tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg
     3781 tcttttgagc gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg
     3841 agaagtccat gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga
     3901 tgaaagggaa ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg
     3961 tagtaaaacc gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt
     4021 tctggtgatg gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg
     4081 tcagcaaatt gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga
     4141 taactcgctc tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt
     4201 tgctgaacaa cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg
     4261 caatatcgac gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc
     4321 ttctctgttg cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta
     4381 cattttcgac cctgcctgcg actggggtga ccgagagtgc gacctagcga tgttaccgct
     4441 gcatactgaa cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc
     4501 cgatttcctt gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt
     4561 atttggcggt cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg
     4621 atatgggttg aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga
     4681 gaaaagaaca cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac
     4741 tgcaggaaaa tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc
     4801 ccctcttccc ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag
     4861 cgcgcctggc gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg
     4921 atgaaaatca cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta
     4981 taccagaaca gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca
     5041 aagtcgagca tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc
     5101 agcaaagaaa tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt
     5161 ttcaaaacag caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc
     5221 gtcgtttcac ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg
     5281 ataggcgctt aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt
     5341 tgtaatgtca accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat
     5401 cgactcagaa cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga
     5461 tatctcccgt cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga
     5521 tctttggtac gcccggcaac cgtggaatgg gccaagccgt caggaagtag tagaacgggt
     5581 tattgcccgt gccatttcac tggttgaaga gacacgtcca ttattaccag gcgtgcgcga
     5641 agctgttgcg ttatgcaaag aacaaggttt attggtggga ctggcctccg cgtcaccact
     5701 acatatgctg gaaaaagtgt tgaccatgtt tgacttacgc gacagtttcg atgccctcgc
     5761 ctcggctgaa aaactgcctt acagcaagcc gcatccgcaa gtatatctcg actgcgcagc
     5821 aaaactgggc gttgacccac tgacctgcgt ggcgctggaa gactcggtaa atggcatgat
     5881 cgcttctaaa gcagcccgca tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga
     5941 tccacgtttt gtattagcaa acgtcaaact ctcatcgctg acagaactca ccgcaaaaga
     6001 ccttctcggt taatgaccag gggcagtgat cacgctgccc ctggttcttt atctgaattt
     6061 gcattcaact gacggattaa tcgtcaattt aagagaaaga gttacaccgt caccacttcc
     6121 gtgcactgta taaaaatcct atactgtacg tatcgacagt ttagtgagtt ttatcatgac
     6181 ggcggaaggt caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct
     6241 gacgcccgtg ctggcacagg gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg
     6301 tttgttaccg gacatcgatc acccaaagtc gtttcttggg cagcgattaa aatggatatc
     6361 aaaaccgatc gcccgcgctt ttgggcatcg tggttttacc cacagtctgc tggcggtatt
     6421 tgcgctgctg gcaacctttt accttaaggt tccggaaggc tggttcattc cggctgatgc
     6481 gctacaagga atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc
     6541 cggtgttccc ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca
     6601 aaagggcaac caactggaac gttttatctg catggcatta tttgtctggt cggtatggat
     6661 gccccattca ttacccgaga acagcgctgt tcgttggtca tcgcaaatga tcaatacctt
     6721 gcagatccag tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatca
     6781 cctttctgga ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct
     6841 tgcgccaaca ctatgactgc tacgcagtga tagaaataat aagatcagga gaacggggat
     6901 gaactttcca ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca
     6961 gacccgccat aaacagtgga gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt
     7021 ggtttttggc cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc
     7081 tgtacagtgg tttaacatcg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat
     7141 gccgttagtc ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt
     7201 aggcaaaatc agttttctga ccatcggtac gcttttgttt accacgctga ttgcggcgct
     7261 ggtcggtgtg ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg
     7321 tgcagaaact gcacgtctga acgccattga aagtaactat gttggtaaag tctctgatca
     7381 gagcgttccg cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg
     7441 agccaatccg acgtcaatta tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc
     7501 attgaaactg ctgaaggatg atgcgccgaa aggggaacgc gtcttaaccg ctatcgatac
     7561 cctacaaagc tgggtgatga aactggttcg ccttgtcatg cagttgaccc cttacggcgt
     7621 tctggctcta atgaccaaag tggttgcagg ttctaacttg caagacatca tcaaactggg
     7681 aagtttcgtt gtcgcgtcct acctcggtct gctgattatg tttgctgtgc atggcattct
     7741 gctgggcatt aatggcgtga gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac
     7801 gtttgccttt accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac
     7861 gcgtcgtctg ggcgttcctg aatccatcgc cagtttcgcc gcctctttcg gtgcaaccat
     7921 tggtcagaac ggctgcgccg gtttgtatcc ggcaatgctg cggtgatggt tgcgcctacg
     7981 gttggcatta acccgctgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt
     8041 agttccgcag gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg
     8101 cctgcgatgg gcctgccagt aaccctggtg gcgctgttaa tctccgttga accgcttatc
     8161 gacatgggcc gtacggcgct aaacgttagt ggctcgatga cagctggcac gctgaccagc
     8221 cagtggctga agcaaaccga taaagccatt ctggatagcg aagacgacgc cgaactggca
     8281 caccgttaat tcttatgctg gcaacggtcc gttttgtata ggggccgttg ctttactttt
     8341 taaatgttcc atcgcggcga tgcatactga ctcata
//
LOCUS       DAZPEU010000168         8339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPEU010000168 DAZPEU010000000
VERSION     DAZPEU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.169"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(168..566)
                     /locus_tag="ACHU2L_003306"
     CDS             complement(168..566)
                     /locus_tag="ACHU2L_003306"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR1358038.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACHU2L_003307"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACHU2L_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR1358039.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..6135
                     /gene="traI"
                     /locus_tag="ACHU2L_003308"
                     /pseudo
     CDS             875..6135
                     /gene="traI"
                     /locus_tag="ACHU2L_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6155..6901
                     /gene="traX"
                     /locus_tag="ACHU2L_003309"
     CDS             6155..6901
                     /gene="traX"
                     /locus_tag="ACHU2L_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR1358040.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6956..7516
                     /locus_tag="ACHU2L_003310"
     CDS             6956..7516
                     /locus_tag="ACHU2L_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1358041.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7647..7859
                     /locus_tag="ACHU2L_003311"
     CDS             7647..7859
                     /locus_tag="ACHU2L_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1358042.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8103..>8339
                     /locus_tag="ACHU2L_003312"
     CDS             8103..>8339
                     /locus_tag="ACHU2L_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1358043.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1914 a         2085 c         2675 g         1665 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc gctggcttcc acacgggtga tgacggacgg
     1261 acagtcagaa acggtgctga ccggtaatct ggtgatggca ctgtttaacc acgacaccag
     1321 tcgcgatcag gaaccacagt tacacacgca tgcggtggtg actaatgtta cgcagtataa
     1381 cggcgaatgg aagacactga gcagtgacaa agtggggaaa acggggttca ttgagaatgt
     1441 gtacgctaat cagattgcct ttggcaggct ctaccgggaa aaactgaaag agcaggttga
     1501 ggcgctgggc tatgaaactg aagtggtcgg taagcacggt atgtgggaaa tgccgggtgt
     1561 accggtggag gccttttccg gacgctcaca gactatccgg gaggccgtcg gggaagacgc
     1621 ctcgctgaaa tcccgggatg tggcggcgct ggatacgcgt aaatccaaac agcacgtcga
     1681 tcctgaagtc agaatggccg agtggatgca gacgctgaag gaaaccgggt tcgacatccg
     1741 ggcatatcgt gacgcagcgg aacaacgggc atatacccgc acgcagacac ccggacccgc
     1801 ttcacaggac gggccggatg tgcagcaggc ggtgacacag gccattgccg gattaagtga
     1861 acgcaaagtg cagtttatgt atacggacct gctggccagg acggtcggca tactgccgcc
     1921 ggaaaatggt gtgattgaac gggcacgcgc cggtatcgat gaggccatca gccgtgagca
     1981 gcttatcccc ctcgatcgtg agaaggggct gttcacgttc gggattcata tgctcgatga
     2041 gctgtcagtc cgggcactca gtcgtgacat catgaaacag aaccgggtga ccgtacatcc
     2101 ggagaaaagt gtcccccgga cggccggtta cagcgatgcc gtcagtgttc tggcacagga
     2161 ccgtccgtcg ctggccattg tgtccgggca gggcggtgca gccgggcagc gtgagcgggt
     2221 ggctgaactg gtcatgatgg cccgggagca ggggcgggag gtgcagatta tcgctgctga
     2281 ccgtcgctcg cagatgaacc tgaagcagga tgaacggctg tccggtgaac tgataaccgg
     2341 acgtcgtcag ttgctggaag gaatggcctt cacgccgggc agtaccgtta tcgtcgacca
     2401 gggcgaaaaa ctctccctga aagagacgtt aaccctgctg gatggtgccg cacgtcataa
     2461 cgtacaggtc ctgataaccg acagcgggca gcgaaccggt acaggcagtg cactgatggc
     2521 catgaaggat gccggggtga acacatatcg ctggcagggg ggagaacagc gaccggccac
     2581 catcatcagt gaaccggacc gtaatgtcgg ctggccggag attttgcggc cagcgtgaaa
     2641 gccggagaag agagcgtggc acaggtcagc ggagtacggg aacaggccat actgacacag
     2701 gccattcgca gtgagctgaa aacacagggc gtgctcggac acccggaggt aaccatgacc
     2761 gccctgtcac cggtctggct ggacagccgg agccgttatc tgcgggatat gtaccgcccg
     2821 ggaatggtga tggagcagtg gaacccggag acacgcagtc atgaccgcta tgtgacagaa
     2881 cgtgtgacgg cgcagagtca cagcctgacc ctgcggaatg cgcagggcga aacgcaggtg
     2941 gtgcgtattt cttccctgga cagcagctgg tcgctgttcc ggccggaaaa aatgccggtg
     3001 gcagacggtg agcgtctgag ggtgacaggg aaaattcccg gactccgcgt ctccggcggt
     3061 gaccgcctgc aggtggcatc cgtcagtgaa gatgcgatga cggttgttgt gccgggacgg
     3121 gctgaaccgg ccaccctgcc tgtgagcgat tcaccgttca ctgcactgaa gctggagaac
     3181 ggctgggtgg aaacgcccgg gcattccgtc agcgacagtg cgacggtttt tgcctccgtc
     3241 acacagatgg caatggacaa tgccaccctg aatggtctgg cccgcagtgg tcgtgatgtc
     3301 cggctgtatt cctcactgga tgaaacccgt actgcggaaa aacttgcccg ccatccctcc
     3361 tttacggtgg tttctgagca gataaaggcg cgtgccggtg agacattgct ggaaaccgct
     3421 atcagtctgc agaaagccgg gcttcacacg ccggcacagc aggccattca tctggccctt
     3481 cctgtgctgg aaagtaaaaa cctggccttc agcatggtgg acctgctgac agaggcgaag
     3541 tcgtttgctg cagaaggaac cggttttgct gacctgggag gggaaatcaa tgcgcagata
     3601 aaacggggtg atttactgta tgtggatgtg gcaaaaggct atggcacagg cctgctggtt
     3661 tcccgtgcgt cgtatgaggc agaaaagagc attcttcgcc atattctcga aggtaaggag
     3721 gcggtcacgc cgctgatgga gagagtacct ggcgaactca tggagaaact gacatcagga
     3781 cagcgcgccg ccacccgtat gatactggaa acgtctgacc gtttcacggt ggtgcagggc
     3841 tatgccggtg tgggtaagac cacacagttc cgggcggtga tgtcagccgt gaacatgctg
     3901 ccggagagtg aacgtccccg tgtcgtgggg ctggtgccca cacaccgtgc ggtcggggag
     3961 atgcgcagcg ccggcgtgga tgcacagaca ctggcgtcct ttctgcatga cacgcagctg
     4021 cagcagcgca gcggagaaac tccggatttc agcaacacgc tgttccttct cgatgagagc
     4081 tctatggtgg gcaataccga catggcacgg gcatacgccc tgattgcggc cggtggcggt
     4141 cgggctgtgg ccagcggtga cacggaccag ctgcaggcca tcgcgcccgg tcagcctttc
     4201 cgtctccagc agacgcgcag tgctgccgat gtggtcatca tgaaggagat tgtgcgtcag
     4261 acgccggaac tgcgggaggc ggtatacagc ctgattaacc gggatgtgga aagggcgctg
     4321 tccgggcttg agcgtgtgaa accgtctcag gtgccacgtc tggagggcgc atgggcaccg
     4381 gagcactccg tgacggagtt cagtcacagc caggaagcga aactggcaga agcgcagcag
     4441 aaggcgatgc tgaaaggcga ggcttttccg gatgtcccca tgacactgta tgaagccatt
     4501 gtccgcgact ataccggcag gacaccggaa gcacgggagc agacgctgat tgtcacgcac
     4561 ctgaatgagg accggcgcgt actgaacagc atgattcatg atgcacggga aaaggccgga
     4621 gaactgggga aggtgcaggt catggtgcct gtcctgaaca cagcgaatat acgcgacggg
     4681 gagctgcgtc gtctctccac ctgggagaat aacccggatg cccttgccct ggtggataat
     4741 gtgtatcacc ggattgccgg tatcagtaag gatgacgggc tgataaccct gcaggatgcg
     4801 gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt cacgctgtac
     4861 accccggaca ccatccgggt ggggaccggt gacaggatac gcttcacgaa gagtgaccgg
     4921 gagcgcggtt atgtggccaa cagcgtctgg acggtgacag cggtttccgg tgacagtgtc
     4981 acactttctg acggtcagca gacccgtgtg attcgccccg gtcaggagcg ggcagagcaa
     5041 catattgacc tggcctatgc catcaccgcc cacggtgcac agggggcgag tgaaaccttt
     5101 gccatcgcgc ttgaaggcac ggaaggtaac cggaaactga tggccggctt tgagtcagcc
     5161 tacgtggccc tgtcgcgtat gaagcagcat gtgcaggtgt acaccgataa ccgtcagggc
     5221 tggacggatg ccatcaacaa tgccgtacag aaaggaactg cccacgatgt gtttgagccg
     5281 aaaccggacc gggaggtcat gaatgcagag cggctgttca gtacggcgcg ggagctgcgg
     5341 gacgtggcgg caggccgtgc cgttctccgt caggcggggc tggccggggg agacagtcct
     5401 gcacggttta ttgctccggg acgtaaatat ccgcaaccgt atgtggcact gccggcgttt
     5461 gaccgtaacg gcaagtccgc cggtatctgg ctgaacccgc tgaccacgga tgacggaaac
     5521 gggctgcggg gattcagcgg tgaaggtcgg gtaaaaggca gcggggatgc gcagttcgtg
     5581 gccctgcagg gcagccgtaa cggagaaagc ctgctggctg ataatatgca ggatggcgtc
     5641 cggattgccc gtgataatcc tgacagtggt gtggtggtaa gaatcgccgg tgaaggccgt
     5701 ccgtggaatc ccggtgccat caccggtggc cgcgtgtggg gggatatccc ggacaacagc
     5761 gtccagccgg gagccggaaa tggtgaaccg gtcacggcag aggtactggc acagcggcag
     5821 gctgaagagg ccatccgccg tgaaacggaa cgtcgcgcag atgaaattgt ccgtaaaatg
     5881 gcagagaaca aacctgacct gccggacggc aaaacagagc aggctgtcag ggaaattgcc
     5941 gggcaggagc gtgaccgggc tgccataact gaacgggaag ccgcgctgcc ggagagtgta
     6001 ctgcgtgaac cacaacgggt gcgggaagcg gtccgggaag ttgcccggga aaatttgctg
     6061 caggagcgtc tgcagcagat ggagcgggat atggttcgtg acctgcagaa agagaaaacc
     6121 ccgggcggag actgatacag gaagacaaac actgatgaca acggataaca cgaacacgac
     6181 acgtaacgat tcactggctg cacggaccga tacctggttg cagtcatttc tggtctggtc
     6241 acccggacag cgggatatca tcaaaacggt ggcactggtg ctgatggtac tggaccacat
     6301 caatctgata ttccagctga agcaggaatg gatgtttctg gccgggcgag gagcctttcc
     6361 gctttttgcc ctggtgtggg ggctgaatct gtcccgtcat gcgcatatcc ggcaaccggc
     6421 cattaaccgg ctgtggggat ggggaattat tgcccagttc gcatattatc tggcaggctt
     6481 tccttggtat gaggggaata tcctgtttgc ctttgcagtg gcggcacagg tgctgacgtg
     6541 gtgtgaaacg cgcagtgggt ggcgtacagc agcggccata ctcctgatgg ccctgtgggg
     6601 gcctttgtcc ggcaccagtt acggtattgc cgggttgctg atgctggcag tcagcaaccg
     6661 gctgtaccgg gcggaagaca gagcggaacg tctggcgctg gtcgcctgcc tgctggctgt
     6721 tattccggcg cttaaccttg ccaccagtga tgcagcggcg gtggccggtc ttgtgatgac
     6781 ggtgctgacc gttggtctgg tgttgtgtgc cggaaaatca ttaccccgtt tctggcccgg
     6841 agactttttc ccgacgttct atgcctgtca tctggctgtg ctgggcgttc tggcgctgtg
     6901 acgggtgtgg tatctttggc cgcaagagga tgattcgtca gaggcagaac acagcatgac
     6961 agagcagaag cgaccggtac tgacactgaa gcggaagaca gagggaacag cgcctgtccg
     7021 cagccggaaa accatcatca atgtcaccac gccaccaaag tggaaggtga aaaagcagaa
     7081 gctggccgag aaagcggccc gtgaagcaga gctggcggca aaaaaagcgc aggccagaca
     7141 ggcgctgtcc atttatctga acctgcccac gctggatgag gctgtgaata ccctgaagcc
     7201 ctggtggccg ggattatttg acggtgacac gccccggctt ctggcctgcg gtatccggga
     7261 cgtgttactg gaagacgtgg cgcagcggaa tatcccgctc tcgcataaaa aactgcgcag
     7321 ggcgctgaag gccatcaccc gttcagaaag ctatctgtgt gccatgaaag ccggtgcctg
     7381 ccggtatgac acggaagggt atgtgacgga gcatatttct caggaggagg aagcgtatgc
     7441 gggagcgcgt ctggcgaaaa ttcgtcgcca gaaccggata aaggcagaac ttcaggccgt
     7501 gcttgatgag aaataaaaaa agcctccccc agagagagga ggcagggaaa taaggcttaa
     7561 aaggaaataa cttccgcttc cgaaaggaag ccagtccact gaattattta tcacaataat
     7621 actgacaact aaagggggac aagaaaatga atggattcag aaacagttca cggaacggcc
     7681 aggtctggcg ttaccagcgt gccggcgggc gggccgttat tctggaagtc agtggacgct
     7741 ggatggaagc ggcagaagca tggcgacggg ctgcctgtgt tgcgcctagg acagactggc
     7801 agcagtttgc ccggaaaagg gctgaacact gccaccggcg ctgccgggga agggtgtaac
     7861 gaaaaaagca gccttcatta ctctaggagt cagaaggctg aaaggagttt atgagatttg
     7921 cttcttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca gagtcttttc
     7981 cgcattaagt cgatttttta tattttgtga ttagtttcac aaaataaggt gttattcaat
     8041 gtgtgctgca ctattcaggg gggctgaaat acaggtggca tttatctgat atggagaata
     8101 acatgaggaa atatattcca ctggcattat ttatcttttc atggccggta ttgtctgcag
     8161 atattcatgg acgggttgtt cgtgtactgg acggtgatac catcgaggtc atggactcac
     8221 tgaaggcggt gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa caggattatg
     8281 gccggtggtc aacagacatg atgaaatcac tggtcgcagg aaaaacggtt accgtcaca
//
LOCUS       DAZPEU010000169         8166 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPEU010000169 DAZPEU010000000
VERSION     DAZPEU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.170"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..983
                     /locus_tag="ACHU2L_003313"
     CDS             <1..983
                     /locus_tag="ACHU2L_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1358044.1"
                     /translation="DRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYD
                     FSHLSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDD
                     SNFAQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIA
                     PYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMV
                     ICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGE
                     ENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1137..2786)
                     /locus_tag="ACHU2L_003314"
     CDS             complement(1137..2786)
                     /locus_tag="ACHU2L_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR1358045.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2937..4286)
                     /gene="ghxP"
                     /locus_tag="ACHU2L_003315"
                     /gene_synonym="yjcD"
     CDS             complement(2937..4286)
                     /gene="ghxP"
                     /locus_tag="ACHU2L_003315"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR1358046.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4992..5456)
                     /gene="soxR"
                     /locus_tag="ACHU2L_003316"
     CDS             complement(4992..5456)
                     /gene="soxR"
                     /locus_tag="ACHU2L_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR1358047.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5542..5865
                     /gene="soxS"
                     /locus_tag="ACHU2L_003317"
     CDS             5542..5865
                     /gene="soxS"
                     /locus_tag="ACHU2L_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR1358048.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5868..7445)
                     /gene="pdeC"
                     /locus_tag="ACHU2L_003318"
                     /pseudo
     CDS             complement(5868..7445)
                     /gene="pdeC"
                     /locus_tag="ACHU2L_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            7874..8155
                     /locus_tag="ACHU2L_003319"
     CDS             7874..8155
                     /locus_tag="ACHU2L_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR1358049.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2272 a         2089 c         1867 g         1938 t
ORIGIN      
        1 acgatagaat tacgagaaga gtttacctta gtaaagaaaa ggatgtatcg attaaggtgg
       61 tatattttat aaataatgtc gccgtccata ataatactat cgaaattcca cagatagtaa
      121 atggtggtta cgatttttca caccttagcc tgaagggtat cgtgattaaa gatgaagatt
      181 tatccaattc gaattttgca ggttgcagac tacaaaacgc tattttccag gactgtaata
      241 tgtataaaac gaatttttat tacgccataa tggaaaaaat actttttgat aattgtattc
      301 tcgatgactc aaatttcgct cagataaaaa tggccgacgg aactctaaat gcatgctccg
      361 ctatgcatgt tcaattctac aatgcagcaa tgaatagagc caatattaaa aataccttcc
      421 ttgactattc aaatttttat atggcgtaca tggctgaggt aaatctttat aaagtaatag
      481 cgccatatgt taatttattt aaagccgacc ttagtttctc taaactcgat ttaattaact
      541 ttgaacatgc tgatctgtct cgcgtcaatc tgaacaaagc aatcctccag aatataaact
      601 taattgatag caaactcttt tgtacgtggc tgacaaatac attcctcgaa atggttatat
      661 gtaccggctc taatatggct aatgttaatt ttaataatgc caatttaagc aactgccatt
      721 tcaactgttc tattttaaca aaagcctgta tgtttaatac ccgtctctat cgggttaatt
      781 tcgatgaggc tagcgtccag ggaatgggca tttccattct ccgtggggag gaaaatatcc
      841 ccattgatag tgataccctg gtaacactac agaaattctt tgaagaagat tgtacctctc
      901 atactggcat gtcacaaact gaggataata ttaatgcagt cgctatgaag attactgcag
      961 atattatgca acacgcagat tgaggttaag tcgaagaaca gccaatgaat tgactttaca
     1021 agagagataa cttatttgtt ttagaatcat gaatgttcgc ccgagctaac ctgttaaagg
     1081 tgcaaaagat tgcacctttt tcagattacc tcgggcctgg ttacgtcgct ttcggcttac
     1141 tgattttcct caattagcaa cgcttcaagc aaatcgagat cgtgcagtaa tttttgcagc
     1201 gtttcgttgc tgatctcccg cgtggcgcgc aggtggtaca gttcagcacg ttcagaacgc
     1261 aatgccgcca gacggaagcg acgctcaagg ttctcttcct gcacggaact ttcaacgtcg
     1321 ttacgtccat cggcgcgacg acgcaggtta ccaatgacgc gagaactgac ctccgtaagc
     1381 aactggttat cgatattctc ttcggtatcc gccgccagac gctcctccat tttctggatc
     1441 gccacaatcg ccacttctgc cgttgccgct cgcgcaatac gttcttcttt caattgttgc
     1501 gaatggtccg cgacttcaat gtgttgtagc agaataggca acatcaccac gccgacaaac
     1561 agcgagaaga gaatgacgcc agccgccagg aacaccagct catagcgcgc cgggaagacg
     1621 ttaccatccg gcaagagcag cgggattgag agcacaccgg ccagagtgat cgccccacgc
     1681 accccagcga aagacgcgat caggatttct cgtgtggtcc acgaaccaaa ctccatcggc
     1741 ttcttcttca ggaagcggtt gctgaacttt ttcatcgtcc acagccagcc gaaacggacc
     1801 agcatcagcg ccgcatatat cagaataata tcggtaaaca gcatccagat ttcgacgtta
     1861 gggtcgattt ctgccgccat cagcgacgtc tccagaatac ccggcagttg cagacctaac
     1921 agcaggaaca ccatgccgtt aaagacaaat tccagcatcg cccaggtgct gtttgcgcgc
     1981 aggcgcattg ccagtggcgc acggcgcatc acaccggagc gggtgatggt catcccggcg
     2041 gcaaccgcag cgaggatgcc cgaaacgcca atatgttcgg caatcagata agaagcgaat
     2101 ggcagcaaga acagcagaac gatctgcgtc gcgggttcat caccgcccca gcggctgagg
     2161 aatcgcagcg agcgaccata cagccagctc accacaaaac cggcaagaat accgccaatg
     2221 gctactttca taaattcgac cgtcgcaccg ccgacagtga agatcatcgt ccccatcgcc
     2281 actgccacgg caaacttcaa cgacaccagg ccggaagcgt cattcatcaa cgcttcgccc
     2341 tgcaaaatgc ccatgatttt tttcgggatg cgcccttcgc caacaatccc ggagagcgcc
     2401 acagcatcgg tcggagaaag taccgccgcc agcgcaaagg cggggatcag cggaatgccc
     2461 ggcaccaccc agtaaataag gaagccaatg ccgaccacgg tgaccaccac cagcgccagc
     2521 gcgaggccga aaatctctcg accatgttcg agaaattcac gggtcggcgt tttccagcca
     2581 tcagcgaaca gcaacggcgg gataaacaag actaaaaaga gttcaggatc aaactccaca
     2641 tgcaaaccaa acgtcggcca cgccagtagc gcaccgatgg cgatttgcat aagcggaagc
     2701 gggatctgaa agggcatgac acgagtgacc accccggaca gcgagaccac gagggtcatt
     2761 atcagtatgg tgaagaagat ttccatgcgt tccctgtttg cgatgttttt tatgatctga
     2821 gaaagacgtt atgcctgaat tctcattttt tcagaatatc gcaaaagctc acacaatgtg
     2881 cggagaaaat aaaaacgggc agccattgcc acccgttttc agaatccgat gaaagattag
     2941 atagcccagc cacccgcata gaaggtcacc agcgccacgg cgataacgac cgtaccgatg
     3001 ttcaacttgc gccattcacc ggaaaccaga cgaccaatca ccagagtcgc gaagccgatc
     3061 atgatgcctg ttacgatgtt acaggtcagc acgatgaata ccgccgtaac cagacccgcc
     3121 atcgcatcaa caaaatcagc aaagtcgatt ttcgccacgt tgctcagcat cagcaggcca
     3181 acgtacatca gcgccggagc cgttgcatac cccggaacga ggtaagaaag cggagagagg
     3241 aacagaatca gcaggaacag cacgccaacg gtgatagccg tcaacccggt tttaccgccc
     3301 gccgccgtac ccgccgcaga ctcgatgtat accgctgccg gagctgcacc caccaggcca
     3361 gagaaaacgc tgctcatgga gtcagtggtc agtgctttcc cgccgtcgat gatctgccca
     3421 tctttatcca gcaggttcgc ctggccggcg acagcgcgga tagttccggt ggcatcaaat
     3481 actgccgtca tcaccagcgc cagaacgctt ggcaggacta caggattcag cgcgcccata
     3541 atgtccaggc tgccaatcag tgaattgccg ttttcatcgc tcaatgaagg catggcgaaa
     3601 acgccggaga aatggacgtt aggatcgaag atcaaaccga caattgagat accgataatg
     3661 gtcagcagaa tgccaccagg gactttcagt ttttccaagc cgatgatcac cgccagacct
     3721 gccagtgaca taatcaccgg gaaggtcgcg aaatcaccca gcgcaacggg cagaccatca
     3781 agcgggtttt taatcaccag accgacacca ttagcggcaa tgagcagcag gaacagaccg
     3841 ataccgatcc ccgtgccgtg cgccacaccg tgcggcaagt tgcgcaaaat ccagctacgg
     3901 atacccgtgg cagaaattac cgtaaacagc acacccatca ggaacacggc acccagcgcg
     3961 acaggtacgc taatatgttg ccccagcacc aggctgaatg cggtaaacgc cgtcagggag
     4021 atggcgcaac caatcgccaa cggcagatta gcccacagac ccatcacgat agaaccgagt
     4081 ccggcaacca gacaggttgc aacgaaaact gccgcaggcg ggaagcctgc tttacccaac
     4141 atacctggaa cgacgatgac cgagtagacc atcgccagaa acgttgttaa cccggcaacc
     4201 acttcctgac ggacagtgct tccacgttgt gaaattttaa accaggcgtc gagtgaaccg
     4261 ccggtacgcg ctgatggcgt agacataaga aaacatcccc tgagagttta attttcgtca
     4321 agatgcgtgg cggacaatcc cctgccagcg aaacgttatc ttcctgctcc aggtttgcgg
     4381 caatttggtg cgtcgcaaaa aagcaaacgt ttagctccgc gcatacaaaa cgggtggtaa
     4441 aaaaaagcaa acgattatcc ggcgatcata gcggcttttt caaccgaagt ttgctgcttt
     4501 tttctctttt attgtcttga cgatgaaaaa ccaggcaatt gatgcgcaaa cgttatcgtc
     4561 gatgtgcaag attgcagcgt tatcgagccg tataccgttc tggattgcga taaaaaatcg
     4621 tctgagatga caaaaatatt gcggctaaaa agtatacttt tacactggcg aagaaacgtc
     4681 ggtgacatac aattaaagca tcaacaccaa ccggaacctc caccacgtgc tcgaatgagg
     4741 tgtgttgacg tcgggggaaa ccctcctgtg ttccagcggg atagagagaa agacaaagac
     4801 cggaaaacaa actaaagcgc ccttgtggcg ctttagtttt gtgtttgcgg ataattccaa
     4861 aagagctcga tcagtcccct cgccccttcg gggagagggt tagggtgagg ggaacaggca
     4921 gcaccagtgc aaatatttac cctcaccccg gccccatagt taacgatgag tcagcttgtc
     4981 cttgtggcgc tttagttttg ttcatcttcc agcaagcgtg cgccggtacc ttcttctcct
     5041 aagcggtcgc ccgggttacg caacgggcaa tcactgcgcg aaaggcagcc acaaccaata
     5101 catccgtcca gttcgtcacg cagcgccact aaggtatgaa tgcgccgatc caactcttct
     5161 cgccattggg acgaaagctg tttccactct ttcgcactta acgtatgccc ttctggcaac
     5221 acgccaaacg cttcaccaat ggtcgccagc ggaatgccaa tacgctgagc gattttgata
     5281 attgcgacat atcgcaacac atcacgttta tatcgccgct gattgccgct gttacggata
     5341 ctagtaatca accctttgct ttcatagaaa tgcagcgccg ataccgccac accgctgcgt
     5401 ttcgccactt cgccaggggt tagcagcgct ttaatgcggg gtaatttctt ttccataaat
     5461 cgctttacct caagttaact tgaggaatta tactccccaa cagatgaatt aacgaactga
     5521 acactgaaaa gaggcagatt tatgtcccat cagaaaatta ttcaggatct tatcgcatgg
     5581 attgacgtgc atattgacca gccgcttaac attgatgtcg tcgcaaaaaa atcaggctat
     5641 tcaaagtggt acttgcaacg gatgttccgc acggtgacgc atcagacgct tggcgattac
     5701 attcgccaac gccgcctgtt actggccgcc gttgagttgc gcaccaccga gcgtccgatt
     5761 tttgatatcg caatggacct gggttatgtc tcgcagcaga ccttctcccg cgttttccgt
     5821 cggcagtttg atcgcactcc cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc
     5881 gttaactccc gcgcgggtaa ttgctccatc cattgcataa acacctgcgg cggcatcgcc
     5941 ttcgcaaaaa accatccctg gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc
     6001 tcctcagttt cgacgccttc agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg
     6061 atgtgttccg caatcaaatg actggttttg tgggtggtca gcgtttcaac aaacgattta
     6121 tcgattttca aaatatcgac attcaatgat ttaaggttat gcaagttaga gtagccaata
     6181 ccaaaatcat caattgccac ttcgtaacca gcctggcgga aagccagaat taatcggcgt
     6241 cattttttcg acatcaagaa atgcatgctc agtcacttca aatttaattt gctgcggacg
     6301 caccgcgtat tgctctgttt tctgattgat tcgcgctatc aaccgtgacg tatgaaaatc
     6361 ggaggccgac aggttaatag aaacatagcg atcggcatgt gttgccaggt acgcgcccag
     6421 atcgcggaag acattatcaa taacataatc agttatctgt tctatcatcc cctccttttc
     6481 tgccagcgga ataaactctg ccggattcat tatttgcccc tgctcaccag gccaacgtaa
     6541 caacgcttca gcgccgatac atttttctgt tttgatatcg attattggct ggtaataaag
     6601 acaaagttga tgtttttcga gggcgcgttg caatttacgt ttgggagata aatagttttg
     6661 tcgaatacgt agccagagta atagcagaac aagactcccc aaaataccgg cgggcaacgc
     6721 aaaaataaga tgattataaa aatgggttat aagacgttga tatgaagtcg caacaatggc
     6781 tgcaattggg cgttttgtcg aataaattgt cgcatataaa tagccatttc tttgtacggt
     6841 taaatccttc aaatgaatca gcggagaaaa cgttgctgcc gaggcctctt tgcttaacga
     6901 gaaaaaggtt ttcatcaccg tatcatacac accccattgc aatgtcgggt catcagacat
     6961 cacttcactc cagaagagag ggttgataac cgccacataa tttccccgct gcatataggt
     7021 cattttatag ccagagaaaa aaggcgtatc gcggtaataa tagatagaaa cgttaggttc
     7081 acgcttataa tcggccggtg caatcgtata gccgtttaca ggcgctatca gcgatgagca
     7141 taaaaaatgg ttatcacgaa ttcattaata taaagatagc cacgaataat attcaacatt
     7201 cgcttttgat gggctggagt gcaaacttgc ccctgatagc gttcagctgc atcgctcact
     7261 aaatctgcct ggcgaatgac cagctcggat ttatccagtg ccagttgagc aaaggttcgg
     7321 agctgattat tcacttctga ttttgcccaa aggaaggcaa tccatagcga aagaatgatg
     7381 ggaaagagaa ctaaaaagat aatgcccggc aacgccagta attggtgtcg tgcacgatga
     7441 ctcattgcta tcaatttcct gcctgtttat acccggccat tgcaaggtga aaccctggct
     7501 accgacgtct ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga
     7561 taccttttta atcgtcacct ggatgaaaat aattcattga tattcaaaat taattttcta
     7621 caggaataca gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataattt
     7681 gtacagcaat aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagttc
     7741 acaaaattaa tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc
     7801 cagagtgtac cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca
     7861 gaggaattgt ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcaacttct
     7921 tagtgccgta atgatgtttc tggccagcac actcaacatc cgttttcgtc ggtctgatta
     7981 tgtcgggctt gcggtgatca gcagcggtct gggcgttgtt tccgcctgtt ggttcgcaat
     8041 ggggttgctt ggcatcacaa tggcggacat caccgctatc tggcacaaca tcgagtcggt
     8101 gatgatagaa gagatgaatc agacaccgcc acaatggcca atgattttga cctgatacgt
     8161 agaagc
//
LOCUS       DAZPEU010000170         8082 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPEU010000170 DAZPEU010000000
VERSION     DAZPEU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.171"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(190..333)
                     /gene="ypaB"
                     /locus_tag="ACHU2L_003320"
     CDS             complement(190..333)
                     /gene="ypaB"
                     /locus_tag="ACHU2L_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR1358050.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            461..2746
                     /gene="nrdA"
                     /locus_tag="ACHU2L_003321"
     CDS             461..2746
                     /gene="nrdA"
                     /locus_tag="ACHU2L_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1358051.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPNIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2980..4110
                     /gene="nrdB"
                     /locus_tag="ACHU2L_003322"
     CDS             2980..4110
                     /gene="nrdB"
                     /locus_tag="ACHU2L_003322"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1358052.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4110..4364
                     /gene="yfaE"
                     /locus_tag="ACHU2L_003323"
     CDS             4110..4364
                     /gene="yfaE"
                     /locus_tag="ACHU2L_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR1358053.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4418..5068)
                     /gene="inaA"
                     /locus_tag="ACHU2L_003324"
     CDS             complement(4418..5068)
                     /gene="inaA"
                     /locus_tag="ACHU2L_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR1358054.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5148..5252)
                     /locus_tag="ACHU2L_003325"
                     /pseudo
     CDS             complement(5148..5252)
                     /locus_tag="ACHU2L_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5247..5489
                     /locus_tag="ACHU2L_003326"
                     /pseudo
     CDS             5247..5489
                     /locus_tag="ACHU2L_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5531..6607)
                     /gene="glpQ"
                     /locus_tag="ACHU2L_003327"
     CDS             complement(5531..6607)
                     /gene="glpQ"
                     /locus_tag="ACHU2L_003327"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1358055.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6612..7970)
                     /gene="glpT"
                     /locus_tag="ACHU2L_003328"
     CDS             complement(6612..7970)
                     /gene="glpT"
                     /locus_tag="ACHU2L_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR1358056.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
BASE COUNT         2073 a         2182 c         1888 g         1939 t
ORIGIN      
        1 cttcgcattt aacaataacc gaatagaaaa caaccatttc gacatcaaca atcgcttaca
       61 ttcgcttata tattgaccac aactgataca tcagattatg tgatgactcg tgcttagatc
      121 aatttttgca atcattagca aaaagattaa taaaccatct atatcaattt atccaaccta
      181 ttctgccgtt caagaaatcg ccgaacagtt atttttaaca aatttttctc ttcccattga
      241 ctttcccgga caccttgtct gacctaaggt gcgcgaaagc cactttttcc ttcctgagtt
      301 atccacaaag ttatgcactt gcaagagggt cattttcaca ctatcttgca gtgaatccca
      361 aacatacccc ctatatatag tgttctaagc agcttccctt actacaggta gtctgcatga
      421 aactattgcg gaaagaattc caaaaacagg tacgacatac atgaatcaga atctgctggt
      481 gacaaagcgc gacggtagca cagagcgcat caatctcgac aaaatccatc gcgttctgga
      541 ttgggcggca gaaggactgc ataacgtttc gatttcccag gtcgagctgc gctcccacat
      601 tcagttttat gacggtatca agacctctga catccacgaa actattatca aggctgccgc
      661 agacctgatc tcccgtgatg cgccggatta tcagtatctc gccgcgcgcc tggcgatctt
      721 ccacctgcgt aaaaaagcct acggccagtt tgagccgcct gcgctgtacg accacgtggt
      781 gaaaatggtc gagatgggca aatacgataa tcatctgctg gaagactaca cggaagaaga
      841 gttcaagcag atggacacgt ttatcgatca cgaccgtgat atgaccttct cttatgctgc
      901 cgttaagcag ctggaaggca aatatctagt acagaaccgc gtgaccggcg aaatctacga
      961 aagcgcccag ttcctttata ttcttgttgc cgcgtgcctg ttctcgaact acccgcgtga
     1021 aacgcgcctg caatacgtga agcgttttta tgacgcggtt tccacattta aaatttcgct
     1081 gccgacgcca atcatgtccg gcgtgcgtac cccgactcgt cagttcagct cctgcgtact
     1141 gatcgagtgc ggtgacagcc tggattccat caacgccacc tccagcgcga ttgtgaaata
     1201 tgtttcccag cgtgccggga tcggcatcaa cgccggacgt attcgtgcgc tgggcagccc
     1261 gattcgcggt ggcgaagcgt tccataccgg ctgtatcccg ttctacaaac atttccagac
     1321 agcggtgaaa tcctgctctc agggcggtgt gcgcggcggt gcggcaacgc tgttctaccc
     1381 gatgtggcat ctggaagtgg aaagcctgct ggtgttgaaa aacaaccgtg gggttgaagg
     1441 caaccgcgtg cgtcatatgg actacggggt acaaatcaac aaactgatgt atacccgcct
     1501 gctgaaaggt gaagatatca ccctgttcag cccgtccgac gtaccggggc tgtacgacgc
     1561 gttcttcgcc gatcaggaag agtttgaacg cctgtatacc aaatatgaga aagacgacag
     1621 catccgcaag cagcgtgtga aagccgttga gctgttctcg ctgatgatgc aggaacgtgc
     1681 gtctaccggt cgtatctata ttcagaacgt tgaccactgt aatacccata gtccgtttga
     1741 tccgaacatc gcgccagttc gccagtctaa cctgtgccta gaaattgccc tgccgaccaa
     1801 accgctgaac gacgtcaacg acgagaacgg tgaaatcgcg ctgtgtacgc tgtctgcttt
     1861 caacctgggc gcaattaata gcctggatga actggaagag ctggcgattc tggcggttcg
     1921 tgcacttgac gcgctactgg attatcagga ttacccgatc ccggccgcca aacgtggagc
     1981 gatggggcgt cgtacgctgg gtattggtgt gattaacttc gcttactatc tggcgaagca
     2041 cggtaaacgc tactccgacg gcagcgccaa caacctgacg cataaaacct tcgaagcgat
     2101 tcagtattac ctgctgaaag cgtctaatga gctggctaaa gagcaaggcg cgtgcccgtg
     2161 gtttaacgaa accacttacg cgaaagggat cctgccgatc gatacctata agaaagatct
     2221 ggatgccatc gctaatgagc cgctgcatta cgactgggaa gcattgcgtg aatcaatcaa
     2281 aacgcacggc ctgcgtaact ccacgctttc tgccctgatg ccgtccgaga cctcttcgca
     2341 gatctctaac gccactaacg gtattgaacc gccgcgcggt tacgtcagca tcaaagcgtc
     2401 gaaagacggt attttgcgcc aggtggtgcc ggactacgag cacctgcacg acgcctatga
     2461 gctgctgtgg gaaatgccgg gtaacgatgg ctacctgcaa ctggtaggta tcatgcagaa
     2521 atttatcgat cagtcgatct ctgctaacac caactacgat ccgtcacgtt tcccgtcagg
     2581 aaaagtgccg atgcagcagt tgctgaaaga cctgctcacc gcctacaaat tcggggtcaa
     2641 aacgctgtat tatcagaaca cccgtgacgg cgctgaagac gcgcaggacg atctggtacc
     2701 gtcaatccag gacgatggct gcgaaagcgg cgcatgtaag atctgatatt gagatgccgg
     2761 atgcggcgta aacgccttat ccggcctacg gctcggtttg taggcctgat aagacgcgcc
     2821 agcgtcgcat caggctccgg gtgccggatg cagcgtgaac gccttatccg gcctacggct
     2881 cggatttgta ggcctgataa gacgcgccag cgtcgcatca ggcacaggat gcggcgtaaa
     2941 atgccttatc cggcattaaa ctcccaacag gacacactca tggcatatac caccttttca
     3001 cagacgaaaa atgatcagct caaagaaccg atgttctttg gtcagccggt caacgtggct
     3061 cgctacgatc agcaaaaata tgacatcttc gaaaagctga tcgaaaagca gctctctttc
     3121 ttctggcgtc cggaagaagt tgacgtctcc cgcgaccgta tagattacca ggcgctgccg
     3181 gagcacgaaa aacacatctt tatcagcaac ctgaaatatc agacgctgct ggattccatt
     3241 cagggtcgta gcccgaacgt ggcgctattg ccgcttattt ctattccgga actggaaacc
     3301 tgggtcgaaa cctgggcgtt ctcagaaacg attcattccc gttcctatac tcatatcatt
     3361 cgtaatatcg ttaacgatcc gtctgttgtg tttgacgata tcgtcaccaa cgagcagatc
     3421 cagaaacgtg cggaagggat ctccagctat tacgatgaac tgatcgaaat gaccagctac
     3481 tggcaccttc tgggcgaagg cacccacacc gttaacggta aaaccgtaac cgttagcctg
     3541 cgcgagctga agaaaaaact gtatctctgc ctgatgagcg ttaacgcgct ggaagcgatc
     3601 cgcttctacg tcagctttgc ttgttccttt gcctttgcag aacgcgaact gatggaaggt
     3661 aacgccaaga ttattcgcct gattgcccgc gatgaagccc tgcacctgac cggtactcag
     3721 catatgctga atctgctgcg cagcggcgcg gatgatccgg agatggcgga aattgccgaa
     3781 gaatgtaagc aggagtgcta tgacctgttt gtccaggcgg cacagcaaga gaaagactgg
     3841 gcggattatc tgttccgtga cggttcgatg attggtctga ataaagatat tctctgccag
     3901 tacgttgaat acatcaccaa tatccgcatg caggcagtcg gtttggatct gccgttccag
     3961 acgcgctcca acccgatccc gtggatcaac acctggctgg tgtctgataa cgtgcaggtt
     4021 gctccgcagg aagtggaagt cagttcttat ctggtcgggc agattgacgc ggaagtggac
     4081 accgacgatt tgagtaactt ccagctctga tggcccgcgt taccctgcgc atcactggca
     4141 cacaactgct gtgccaggat gaacaccctt cccttctggc ggcgctggaa tcccacaatg
     4201 tggcggttga gtaccagtgc cgcgaaggtt actgcggctc ctgtcgcacc cgactggtcg
     4261 caggtcaggt ggactggatt gccgaaccgt tagcctttat tcagccgggg gaaattttgc
     4321 cctgttgttg ccgggcaaaa ggcgatattg aaatcgagtt gtgatcgggt actagcgcca
     4381 gatacgacgc ttacgcgtat tatctggcct acaacgatta catggcgtag taatacgctt
     4441 tcacctgctc ccagtccgct ttagggatcg gctccagata tttttccagc tggcggaagt
     4501 catgattaat cgctttatcg cgacgcaagc ggcgacgact tttctccaga tcaagaaaac
     4561 cagcttctgc cttaccttct gttttcacat agatatggcg aacatagcaa cagccatgct
     4621 gacgattaac gctatgcatt ttcttaaacg ccagtgccac tgctttcaac atggcttgcc
     4681 gtacttcgtc agaataaggc gatactgcat gccgggcata ccagtcagca atgctgatga
     4741 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     4801 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     4861 cctcacggac aatcgttggt cggccgaacg gataacgtac tgaatgaaac agatgatgcg
     4921 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     4981 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     5041 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     5101 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     5161 tttgccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     5221 gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag tcggatacag
     5281 cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta acgctgaaaa
     5341 gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga cagatttcgt
     5401 tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa atgtgcatgg
     5461 aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg aacgccatcc
     5521 tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa tcagtaaaca
     5581 gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca tcaggagtgt
     5641 attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta ttctgctggg
     5701 catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc tcaatcaaca
     5761 tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg gctcccggct
     5821 taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc tgctgcgttt
     5881 cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc attttgggtt
     5941 ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa taaactttat
     6001 cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc gcaatatcct
     6061 tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg atatttttcc
     6121 cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg tgcacccgga
     6181 agtcggactt acccattggg aaacgccccg gataagtctg tactttttta ccgttttcaa
     6241 tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa tctatcgcgt
     6301 agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca gtaacacgat
     6361 cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc aaatcctgtt
     6421 ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta tgctccggca
     6481 aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc gccgccattg
     6541 cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt ttcagcttca
     6601 atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg acgtttttcg
     6661 ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat cattaccata
     6721 aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct cgccgccacc
     6781 gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc cgcttttttc
     6841 ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat caggaagccg
     6901 ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat ccagtaaacg
     6961 atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg gttaccacgg
     7021 aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc atactcatag
     7081 aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt cagataagtc
     7141 ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc gatggcgatg
     7201 taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc ggtcagctcc
     7261 tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc gatcggcggc
     7321 aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc caccagaatg
     7381 gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc catccccagc
     7441 aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca cactgacaca
     7501 atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca cggcggccac
     7561 cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc aatgctcgac
     7621 gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat caaacctgcg
     7681 ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa tttcgaaaat
     7741 ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga gaatccctgc
     7801 tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta agccgcatag
     7861 ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg atcgatctcc
     7921 gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat tgatagcctc
     7981 cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac ctgccgtttc
     8041 ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tg
//
LOCUS       DAZPEU010000171         7989 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPEU010000171 DAZPEU010000000
VERSION     DAZPEU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.172"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            310..813
                     /gene="dps"
                     /locus_tag="ACHU2L_003329"
                     /gene_synonym="pexB"
     CDS             310..813
                     /gene="dps"
                     /locus_tag="ACHU2L_003329"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR1358057.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1217..1963
                     /gene="glnH"
                     /locus_tag="ACHU2L_003330"
     CDS             1217..1963
                     /gene="glnH"
                     /locus_tag="ACHU2L_003330"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR1358058.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2102..2761
                     /gene="glnP"
                     /locus_tag="ACHU2L_003331"
     CDS             2102..2761
                     /gene="glnP"
                     /locus_tag="ACHU2L_003331"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR1358059.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2758..3480
                     /gene="glnQ"
                     /locus_tag="ACHU2L_003332"
     CDS             2758..3480
                     /gene="glnQ"
                     /locus_tag="ACHU2L_003332"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR1358060.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3741..5965
                     /gene="ybiO"
                     /locus_tag="ACHU2L_003333"
                     /pseudo
     CDS             3741..5965
                     /gene="ybiO"
                     /locus_tag="ACHU2L_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5962..6888)
                     /gene="rlmF"
                     /locus_tag="ACHU2L_003334"
     CDS             complement(5962..6888)
                     /gene="rlmF"
                     /locus_tag="ACHU2L_003334"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR1358061.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7164..7424
                     /gene="mcbA"
                     /locus_tag="ACHU2L_003335"
     CDS             7164..7424
                     /gene="mcbA"
                     /locus_tag="ACHU2L_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR1358062.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7689..>7989
                     /locus_tag="ACHU2L_003336"
     CDS             7689..>7989
                     /locus_tag="ACHU2L_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1358063.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1953 a         2039 c         1986 g         2011 t
ORIGIN      
        1 acattaatta aatttacatt tctgtatggt tatgcataac catgcagaat ttctcgctac
       61 ttttcctcta caccgtattt atatatcgaa ttatgcaaaa gcatatttat tccgaaaatt
      121 cctggcgagc agataaataa gaattgttct tatcaatata tctaactcat tgaatcttta
      181 ttagttttgt ttttcatgct tgttaccact attagtgtga taggaacagc cagaatagcg
      241 gaacacatag ccggtgctat acttagtctc gttaattact gggacataac atcaagagga
      301 tatgaaatta tgagtaccgc taaattagtt aaatcaaaag cgaccaatct gctttatacc
      361 cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag agttgctgaa tcgccaggtt
      421 atccagttta ttgatctttc tttgattacc aaacaagcgc actggaacat gcgcggcgct
      481 aacttcattg ccgtacatga aatgctggat ggcttccgca ccgcactgat cgatcatctg
      541 gataccatgg cagaacgtgc cgtgcagctg ggcggtgtag ctctggggac cactcaagtt
      601 atcaacagca aaaccccgct gaaaagttac ccgctggaca tccacaacgt tcaggatcac
      661 ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg acgtacgcaa agcgattggc
      721 gaagcgaaag atgacgacac cgcagatatc ctgaccgccg cgtctcgcga cctggataaa
      781 ttcctgtggt ttatcgagtc taacatcgaa taaatccatc gctgatggtg cagaacttta
      841 gtacccgata aaagcggctt cctgacagga ggccgttttg ttttgcagcc cacctcaacg
      901 cacttattta gtgcatccat ctgctatccc cagctgatta agtaaatttt ttgtatccac
      961 atcatcacac aatcgttaca taaagattgt tttttcatca ggttttacgc taaataatca
     1021 ctgtgttgag tgcacaattt tagcgcacca gattggtgcc ccagaatggt gcatcttcag
     1081 ggtattgccc tataaatcgt gcatcacgtt tttgccgcat ctcgaaaaat caatgagttg
     1141 caaaattggc acgatttttt catatatgtg aatgtcacgc aggggatcgt cccgtggata
     1201 gaaaaaagga aatgctatga agtctgtatt aaaagtttca ctggctgcac tgaccctggc
     1261 ttttgcggtt tcttctcatg ccgcggataa aaaattagtt gtcgcgacgg ataccgcctt
     1321 cgttccgttt gaatttaaac agggcgataa atatgtgggc tttgacgttg atctgtgggc
     1381 tgccatcgct aaagagctga agctggatta cgaactgaag ccgatggatt tcagtgggat
     1441 cattccggca ctgcaaacca aaaacgtcga tctggcgctg gcgggcatta ccatcaccga
     1501 cgagcgtaaa aaagcgatcg atttctctga cggctactac aaaagcggcc tgttagtgat
     1561 ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat ctcgacggga aagtggttgc
     1621 tgtgaagagc ggtactggct ccgttgatta cgcgaaagca aacatcaaaa ctaaagatct
     1681 gcgtcagttc ccgaacatcg ataacgccta tatggaactg ggcaccaacc gcgcagacgc
     1741 cgttctgcac gatacgccaa acattctgta cttcatcaaa accgccggta acggtcagtt
     1801 caaagcggta ggtgactctc tggaagcgca gcaatacggt attgcgttcc cgaaaggtag
     1861 cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc ctgcgcgaga acggaactta
     1921 caacgaaatc tacaaaaaat ggttcagtac tgaaccgaaa taataacgct acacctgtaa
     1981 agcgcactgg cagttccctc tcccccctgg ggagaggatt agggtgaggg gcgcaaaccc
     2041 gctccggggc cattaattac cctgaatttg attatttaca ccacggtaac aggaacaaca
     2101 tatgcagttt gactggagtg ccatctggcc tgccattccg cttctgattg aaggtgccaa
     2161 aatgaccctg tggatttcgg tcctcggtct ggctggcggt ctggtaatcg gattgctggc
     2221 aggttttgca cgcaccttcg gtggttggat agccaaccac gtcgcgctgg tctttattga
     2281 agtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt atctatttcg ccctgccgat
     2341 ggcgtttaac gacttacgca tcgacccatt tactgcggcg gtggtcacca tcatgatcaa
     2401 ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg ctgtctatcc acaaaggttt
     2461 tcgtgaagca ggactggcgc tcggtctttc acgttgggaa accattcgct acgtcatttt
     2521 accgctggca ctgcgtcgta tgctgccgcc gctgggtaac cagtggatca tcagcattaa
     2581 agacacctcg ctgtttattg ttatcggcgt ggcggaactg acccgtcagg ggcaagaaat
     2641 tattgccggt aacttccgcg cccttgagat ctggagcgcc gtggcggtgt tctatctgat
     2701 tattaccctg gtgctgagct ttattctgcg tcgtctggaa agaaggatga aaatcctgtg
     2761 attgaattta aaaacgtctc caagcacttt ggcccaaccc aggtgctgca caatatcgat
     2821 ttgaacattg cccagggcga agtcgtggtg attatcgggc cgtccggttc cggtaaatcg
     2881 accctgctgc gctgcatcaa caaactggaa gaaatcacct ccggcgatct gattgtcgat
     2941 ggcctgaagg tcaacgatcc gaaagttgac gagcgcctga ttcgccagga agcaggtatg
     3001 gtgttccagc agttttacct cttcccgcat ctgacggcgc tgggaaacgt catgtttggt
     3061 ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa aactggcgcg tgagctgctg
     3121 gcgaaagtcg gtctggcaga acgtgcacat cactaccctt ccgaactttc tggtggtcaa
     3181 cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc cgaaaatgat gctgtttgat
     3241 gaaccgactt ccgctcttga cccggaactg cgccatgaag tgctgaaggt tatgcaggat
     3301 ctggctgaag aagggatgac gatggtgatc gtgacccacg aaatcggttt tgccgagaaa
     3361 gtagcttcgc ggctgatctt tatcgacaaa ggccggattg cggaagatgg caatccgcag
     3421 gtgttgatca agaacccgcc gagccagcgc ttgcaggaat ttttgcagca cgtctcttaa
     3481 taagacacat tgcctgatgc gctacgctta tcaggcctac aggatatctg gcaacttatt
     3541 aaaattgcat gaacttgtag gacggataag gcgttcacgc cgcatccggc aaaaaagccc
     3601 gcacgttgtc agcaacctgc ttaatatccc ttcctccctt tcacccgaaa gggaggcaca
     3661 ccagattcct ctcatttaaa atcgcccctc ccccagcatc tatacttatc tttttgctct
     3721 gttttctcac tggaggagtc atgcggtgga tcctgttcat cctcttctgc ctgctgggcg
     3781 cacctgccca cgcggtatcc atacccggcg ttacaaccac aacgacaacg gactcaacga
     3841 ctgaaccggc cccggaaccg gatatcgaac aaaaaaagcg gcctatggcg cactggcgga
     3901 tgtgctggat aacgacacct cgcgtaaaga gttgatcgac cagttgcgta ctgtcgccgc
     3961 gacgccccct gcggaaccgg tcccgaagat cgtgccgccg acgctggttg aagagcaaac
     4021 cgtgctgcaa aaggtcaccg aagtcagccg ccattatggt gaagcccttt ccgcccgctt
     4081 cgggcaactt tatcgcaata tcaccggctc cccgcataag ccatttaatc cacaaacctt
     4141 cagcaatgcg ctgagccatt tttcaatgtt agcggtatta gtgtttggtt tttactggct
     4201 gattcgcctg tgcgcactgc cgctatatcg caaaatgggt cagtgggcga ggcaaaaaaa
     4261 tcgtgagcgc agcaactggt tgcagcttcc ggcgatgatt attggggcgt ttattatcga
     4321 cctgctgtta ctggccctga cattatttgt cggccaggta ttaagcgaca acctgaatgc
     4381 aggcagtcgc accatcgctt tccaacaaag tttgtttctc aacgcctttg ccctgattga
     4441 atttttcaaa gccgtactac gcctgatttt ttgcccaaac gtggcggagc tgcgcccgtt
     4501 cacgattcag gacgagagcg cccgttactg gggtcgtcgc ctgagctggt taagcagcct
     4561 gattggctat ggcctgattg tggccgtgcc gattatctct aatcaggtga atgtacagat
     4621 aggtgcgctg gcgaacgtca tcattatgct gtgcatgacc gtctgggcgt tgtacctgat
     4681 cttccgaaat aaaaaagaga ttacccagca tatgctcaac tttgcggagc attcgctggc
     4741 ttttttcagc ctgtttatcc gcgcctttgc gctggtgtgg cactggctgg caagcgccta
     4801 ttttatcatg ctgtttttct tttcgttgtt cgatccgggc aacagtctga aatttatgat
     4861 gggtgcaacg gtgcgcagcc tggcgattat tggtatcgca gcgtttgttt ccggtatgtt
     4921 ttcccgctgg ctggcgaaaa ccatcaccct ctcgccacat actcagcgta actatccgga
     4981 gctacaaaaa cggctgaatg gctggctttc ggcggcgctg aaaacggcgc gtattctgac
     5041 cgtctgcgtg gcagtaatgt tgctgttgag cgcatgggga ttgttcgatt tctggaactg
     5101 gctgcaaaac ggcgcggggc agaaaaccgt agatatcctg atccgcatcg cactcattct
     5161 tttcttctcg gcggttggct ggacggtgct cgccagtttg atcgaaaacc ggctggcttc
     5221 ggatattcat ggccgcccgc tacccagcgc ccgtacgcgt accctgctga cgctgttccg
     5281 taacgcgctg gcggtgatta tcagtaccat caccatcatg attgtgttgt cggaaatcgg
     5341 cgtcaatatc gcgccattgc tggcaggtgc cggggcatta ggtctggcta tctcgtttgg
     5401 ttcgcaaacg ctggtaaaag atattatcac cggggtattt attcagtttg aaaacggcat
     5461 gaacactgga gatttggtga ctatcgggcc gttgaccggc acagtggaac ggatgtcgat
     5521 tcgctccgtg ggcgtgcgcc aggataccgg ggcgtatcac atcattccgt ggtcttcgat
     5581 aaccaccttt gctaacttcg tccgcggtat tggttcggta gtggcaaact acgatgttga
     5641 tcgccatgaa gatgctgata aagccaatca ggcactgaaa gatgcggtaa cggaattgat
     5701 ggaaaacgaa gaaattcgcg ggctgattat tggtgaaccg aactttgccg ggattgtcgg
     5761 cttaagcaat accgcgttta cactgcgtgt ttcgttcacc acgctgccac tcaaacagtg
     5821 gacggtacgc tttgccctcg acagccaggt gaaaaaacat ttcgacctgg cgggcgttcg
     5881 cgcgccagtg cagacttatc aggtgctgcc tgctccgggc gcgaccccgg ctgaaccgct
     5941 gccgccgggg gagccaacgc tttaacgttg gcgattaaca aaacggcggc gctgctcgtc
     6001 gttcataaag gtccaggcaa taaagcgact ctgcttttgc ccctgggcca tctctttttt
     6061 taccaccttc accgcgccca cgtcggtcag agcacgatac aacggcggta agttttcacc
     6121 acgagatacc agcgaggtaa accacatcac ctgcttcgcg aagcctttgc tctcttcaat
     6181 catctttttg ataaagttga cttcaccgcc ttcacaccac aactcctgtt gctgaccacc
     6241 aaagttcagt gcatcgtctt tgttcagccc caggttacgg cgtttacgct cactacctgc
     6301 ccgcgccgca gcggcggaat cgtggaatgg cgggttacac aaggtcgcgt cgtattgctc
     6361 gtttttgtgg atgatgccgt taaaaatcgc cccactctct ttttgccgac gcagacgaat
     6421 ggcgcggtta agccccggat tagcactgat aatcgcctgc gcactgctta acgcctggct
     6481 gctggtttca ctaccggtaa aacgccagcc atattcatgt acgccaatca gcggataaat
     6541 acagttcgca ccaacgccga tatccagaat gctggcattc gctggaattg ttccgcttgc
     6601 ttcagccagt aaatcggcaa ggtgatgaat ataatccgcc cggcccggta ctggtgggca
     6661 gagaaaaccg tcggggatat cccagttcgc tacggcgtaa aaatgggcca gcaacgcctt
     6721 attgagcgcc ttcaccgcca gcggattggc aaagtctacg ctttgctccc ctgcgggtgt
     6781 aagcgtgagg aattgcctga gttcaggatt gacctgacaa agcgtggcga gatcgtagcg
     6841 gctgtgatga cggttgcgcg gatgcaaccc cggtttctgg gcggacatag cattctcctt
     6901 taagcagcgg cgtaagatac ccgcataacg cgacagggta aatatctggc tgcgtttatc
     6961 attccccaaa accttaacat ggctatggtt tatggcaatt catcataaaa atcagcatta
     7021 tgcatgtcat tgatttgtct tattttgtaa aaatatatcg ttcaaaaaat aagcatttct
     7081 ctgtcaattt tttgcgtcat ttgcatcgcg caggcgacct acacttatca ggcactaccc
     7141 gttgtattta tgaggatgtc attatgaaaa agtgcctcac actactgatt gccaccgtcc
     7201 tgagcggaat ctctctcacg gcttatgccg cgcaaccgat gagtaacctg gacagcggtc
     7261 agctacggcc cgccggtacc gtttcggcaa ctggcgcatc aaacctaagc gatctggagg
     7321 ataaactggc agaaaaagcg cgcgaacaag gcgcgaaagg ttatgtcatt aattccgcag
     7381 gcggaaatga ccagatgtta ggtactgcaa ccatctacaa ataaccccac caacgtaact
     7441 gcattcgcct ctggatgcag ttttcgcctg ttttgtaatc aaaaaacatc aaatatgaac
     7501 gcaatgtaaa taaatgtatt tctttttcgc gcaatgggtg atagaaaatc gcatcaagtg
     7561 ataatgctta tcaaaattat tatcacttgc acgaacacta tcacgggatt aacagtggca
     7621 tcgcatccgc agagatgctt tctcgtggca gtgaaaattt caacatataa gaaaaagtca
     7681 cctgcaaaat ggaaaacaat cgcaatttcc ctgccagaca atttcattcg ctcacgttct
     7741 ttgccggtct ttgtattggt attacgcctg tggcacaggc actcgccgcc gaagggcaag
     7801 ctaacgcgga tgacacgctg gttgtcgaag catcaacgcc ttcgctttat gcgccacaac
     7861 aatctgccga tccgaaattc tcgcgtccgg tagcggatac tacccgcacg atgacggtga
     7921 tttctgaaca agtgattaaa gatcagggcg caaccaacct taccgacgcg ctcaaaaacg
     7981 tccccggcg
//
LOCUS       DAZPEU010000172         7847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPEU010000172 DAZPEU010000000
VERSION     DAZPEU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.173"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(108..848)
                     /gene="cysQ"
                     /locus_tag="ACHU2L_003337"
     CDS             complement(108..848)
                     /gene="cysQ"
                     /locus_tag="ACHU2L_003337"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR1358064.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            1038..2981
                     /gene="cpdB"
                     /locus_tag="ACHU2L_003338"
     CDS             1038..2981
                     /gene="cpdB"
                     /locus_tag="ACHU2L_003338"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR1358065.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3107..3487)
                     /locus_tag="ACHU2L_003339"
     CDS             complement(3107..3487)
                     /locus_tag="ACHU2L_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR1358066.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3576..4436
                     /gene="qorB"
                     /locus_tag="ACHU2L_003340"
     CDS             3576..4436
                     /gene="qorB"
                     /locus_tag="ACHU2L_003340"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1358067.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4545..5510
                     /locus_tag="ACHU2L_003341"
     CDS             4545..5510
                     /locus_tag="ACHU2L_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1358068.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5618..6280
                     /gene="ytfE"
                     /locus_tag="ACHU2L_003342"
     CDS             5618..6280
                     /gene="ytfE"
                     /locus_tag="ACHU2L_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR1358069.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6325..7737)
                     /gene="cycA"
                     /locus_tag="ACHU2L_003343"
                     /gene_synonym="dagA"
     CDS             complement(6325..7737)
                     /gene="cycA"
                     /locus_tag="ACHU2L_003343"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR1358070.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
BASE COUNT         1996 a         2062 c         1986 g         1803 t
ORIGIN      
        1 attgaatttt tgcactttaa atacaatctc tttttattta taacttttta ggttgggata
       61 aatcaggatg gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatggac
      121 actctgaacc ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
      181 cagtcgtgaa cgtgcgctcc ggcagctgca gccacagcat gtccggcggc ggtgtcccaa
      241 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
      301 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
      361 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
      421 tgaatcagct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
      481 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
      541 gcaatgttaa cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg
      601 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
      661 gaaaggaccg gaatatccgg tgccagcgta cgtaaaccgt ccatgataac ggtgtgagcg
      721 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
      781 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
      841 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
      901 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
      961 cattcttcac tgttctataa gtaaggcgtt tattctccct tttctttcgt attcccgatg
     1021 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
     1081 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
     1141 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
     1201 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
     1261 gcgatttgat tcaggggagt ccgctggccg attacatgtc ggcgaaagga ttaaaagcag
     1321 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
     1381 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
     1441 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
     1501 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
     1561 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggaa
     1621 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
     1681 aaggtgccga tgtcattgtc gttctggcgc attccgggct gtctgccgat ccgtataaag
     1741 taatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
     1801 ttggccatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
     1861 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
     1921 ttggggtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
     1981 aagcggaagc acggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca
     2041 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
     2101 caatcggtaa atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga
     2161 ccgtgcaagt cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
     2221 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
     2281 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
     2341 ccgatcttta tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga
     2401 aagagtggct ggagtgctcc gccggacagt ttaaccagat tgctcctaac agcacgaaac
     2461 cacagtcact catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg
     2521 tgaattatca gattgatgtt acccaacccg cccgctatga cggcgagtgc cagatgatta
     2581 atgccaatgc agagcggatt aagaacctga cctttaatgg caagccgatt gatccgaacg
     2641 ctatgttcct ggttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
     2701 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
     2761 ttgctgatga gtcgaaacgt gcgggggaaa ttcatccggc ggcagataac aactggcgtt
     2821 tagcaccgat agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata
     2881 aagccgcagc gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg
     2941 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
     3001 ataaatcaac cgcatccggc atttattggc ggatgcgatg ctgccgtatc ttatccaccc
     3061 tacaagccat gaaccgtaga ccggataagc gcagcgcatc cggcagttat gccgcacgtt
     3121 catcccgcac cgccaacacc tcgggcaaat tcaactcaat ccagtccgcc agtgcagcaa
     3181 ccttttcgct cacctgctcg cccagcggcg tgaggctata ttccacatgc ggcggcacca
     3241 ccggatacgc gatacggtta agaaaaccat cctgttctaa cgcctgtaac gactgcgcaa
     3301 gcatcttttc actcacccca ccgattttgc gccgcaggtc gctaaagcga tgagtacctt
     3361 cgcgtagcgc caccagaatc aacacccccc aacggctggt gacgtgtttc aacacctcgc
     3421 gcgacgggca ctgttccgca aagagattac cctctttcag ttgttgtgac aggctaacct
     3481 gactcatttc atacttacct ttttgcacgt acttactaaa agtaagttta ggtgttagcg
     3541 tatttaaaca caagacaaaa cgatggagac ttcccatgat cgctattact ggtgccactg
     3601 gccaacttgg tcactatatt attgaatcct tgatgaaaac ggttcctgcc agccaaatag
     3661 tggctatcgt tcgtaatccg gcaaaagccc aggccctggc agcacaaggc attaccgtgc
     3721 gtcaggctga ctacagcgat gaagccgcac tgacatctgc acttcaggga gtggaaaaac
     3781 tactgctgat ctcttccagc gaagtgggtc aacgtgcccc gcagcatcgt aatgttatta
     3841 atgccgcaaa ggcggctggc gtgaaattta tcgcttatac cagcctgcta catgcagata
     3901 cctccccgct cggcctcgcc gatgagcaca tcgagacgga gaaaatgctg gctgattctg
     3961 gcatcgttta caccctgctg cgcaacggct ggtacagcga aaactacctc gccagcgccc
     4021 cggcagcact ggaacacggc gtatttatcg gtgcggctgg cgatggcaaa atcgcctcag
     4081 caacgcgggc agattatgcg acagctgcgg cacgcgtgat tagcgaagcc ggtcacgaag
     4141 gcaaggttta cgaactggcg ggcgatagtg cctggacgtt gacacagtta gcggcagagc
     4201 tgaccaaaca gagcggcaaa caggttacct atcaaaatct gagcgaagcc gatttcgccg
     4261 cggcactgaa aagcgtcgga ctgcccgacg gactggcgga tatgctggcg gattctgacg
     4321 ttggcgcatc gaaaggtggt ctgtttgata acagcaaaac gcttagcaaa ttgattggct
     4381 gcccaacgac aacgttagcc gaaagcgtaa gccatctttt taatgttaat aactagttaa
     4441 ttaaagtggc atcctcccgc atcctctctg ataatgacgg gatgccggga gcaatcatgt
     4501 ctgcttcctg aacgtttctt ctgacagacc aatggatgcc agtaatgatt agcggcgtgc
     4561 tgtacgccct gttagcaggg ttgatgtggg ggcttatttt tgtcgggccg ttgatcgtgc
     4621 cggaataccc ggcgatgttg cagtcgatgg ggcgttatct ggcgttaggg ttaattgcgc
     4681 tacccattgc ctggctggga cgcgtgcgtc tgcgtcagtt ggcgcgtcgg gactggctta
     4741 ccgccttgat gctcactatg atgggcaacc tcatttatta cttctgcctt gccagtgcca
     4801 ttcaacgtac tggcgcgcct gtttccacga tgattatcgg caccctgccg gtggtgattc
     4861 ctgtctttgc caatctgctt tatagccagc gcgacggcaa actcgcgtgg ggaaaactcg
     4921 ccccggcact gatttgtatt ggcatcggcc tggcgagtgt gaatattgct gagttaaacc
     4981 acggactccc cgattttgac tgggcacgtt atacctctgg catcgtgcta gcgttagttt
     5041 ccgtggtctg ctgggcatgg tatgccctgc gcaacgcccg ctggctgcgg gaaaatcccg
     5101 acaaacatcc gatgatgtgg gcgacggcgc aggcgctggt cacgctgccg gtttctctca
     5161 tcggctatct cgtcgcctgt tactggctga atatacaaac gccggacttc tccttacctt
     5221 ttggcccccg tccgctggtg tttattagtc tgatggttgc gatagccgtg ctttgctcat
     5281 gggttggcgc actctgctgg aacgtcgcca gccagcgatt accgacagtg attctcgggc
     5341 cgctgattgt tttcgaaacg ctggcaggtt tgctgtacac ctttttactc cgccagcaaa
     5401 tgccgccgct aatgacgctg agcggtatcg cgctgttagt gattggcgtg gtcatcgcgg
     5461 tcagagcaaa accagaaaaa cctttaactg aatctgtctc agaaagttga catgctggca
     5521 gtgagttaaa taagcctctg ctacgtaagg gttatagctt ttgccttaaa ggtgtattta
     5581 aaatacatct tatcttatta agaatgaggt atcagctatg gcttatcgcg accaaccttt
     5641 aggtgaactg gcgctctcta tccctcgcgc ttcggctctg tttcgtaaat atgatatgga
     5701 ttactgctgt ggcggtaagc agacgctggc gcgcgcggcg gcacgtaaag aactggatgt
     5761 tgaggtcatt gaagctgaac tggcaaagct tgctgaacaa ccgattgaga aagactggcg
     5821 tagcgccccg ctggcagaaa tcattgacca tatcatcgtg cgctaccacg atcgtcaccg
     5881 cgagcagctg ccggagctga tcctgcaagc gactaaagtc gagcgcgttc acgccgacaa
     5941 accgagcgtg ccaaaagggc tgacaaaata cctgaccatg ctgcatgaag agctttccag
     6001 ccacatgatg aaagaagagc agatcctctt cccgatgatc aaacaaggca tgggcagcca
     6061 ggcaatgggg ccaatcagcg taatggaaag cgagcacgat gaagcgggcg aactgctgga
     6121 agtgatcaaa cacactacca ataacgtcac accgccgcca gaagcctgca ccacctggaa
     6181 agcgatgtat aacggcatta atgaactgat tgatgacctg atggatcaca tcagtctgga
     6241 aaacaatgta ctgttcccac gcgccctagc gggtgagtga tctaaaagct ggatggcatt
     6301 gcgccatcca gcatgataat gcggttattt ccgcagttca gcagcccgct tcttaccaat
     6361 aaacagccag cccaacccca gcgcgataaa ccacagcggg gtaaccagca gcgcctggcg
     6421 agtgtcatct tccagtgtca gcaacacgac cacgaacaca aagaacgcca tacataccca
     6481 gcacatcagc ttgccgagcg gcatcttgta gatcgacttt tcatgcagat gaggacgctg
     6541 tttgcggtac accaggtaag agcaaaggat aatcgtccag acgaacataa acagaatcgc
     6601 ggaaacggtt gtaatcatcg tgaacgcgcc aatcacgcta ggattcacat acaacatcac
     6661 cacgccgccg agcagacaga tacacgagaa ggtcaaccct ttcgcgggta ctgcgcgctt
     6721 agaaagttta gcgaacgctt tcggcgcaac accttcctgc gccagaccaa acagcatacg
     6781 gctggtagag aagacaccgc tgttagcgga agacgccgca gaggtcagca ccacaaagtt
     6841 gatcacgctg gcagccgcag gcaaacctac caacacaaac agctcaacga acgggctttt
     6901 ctccgggact accgaactcc acggtgtcac ggacataatc acaatcagcg cgaagacgta
     6961 gaacataatg atacggatcg gaatggagtt aatcgcgcgt ggcagtgatt tctccggatc
     7021 tttggtttcc gcagctgttg tacctaccag ctcaatcccc acgaaagcga aaaccgctat
     7081 ctggaatccg gcaaagaagc cacttaaacc tttcgggaac cagccgccgt cattccacaa
     7141 atgcgcgaat gacgcttcca caccggtcgg tgactgaaag tgcatcgcca ccatgaccag
     7201 gccgacgaca atcagcgaca cgatggcgac gattttgatc atcgcaaacc agaactccat
     7261 ctcaccgaac attttcacgg tggcaaggtt gagcgtcagc agcagcacta tcaccgccag
     7321 cgaggcgacc cagtcggaga gatcggggaa ccagaactga gcataagccg tgatcgccac
     7381 cacgtctgcc ataccggtta caacccagca gaaccagtaa gtccagccgg tgaaatatcc
     7441 cgcccacggc cccagtaaat cggaagcgaa gtcactaaaa gatttgtatt ccagattcga
     7501 aagcagcaat tcccccattg cccgcatcac gaaaaagagc ataaaaccaa tgatcatata
     7561 aacgaaaatg atcgacggcc cggcaaggct aatcgttttg ccggacccca taaacaaccc
     7621 agtaccaatg gcacctccaa tggcaataag ctgaacatgt cggtttgtga gattgcgccg
     7681 tagcgactgt tcagccggag cctgatcatc ggcaacgact tttacctgat ctaccatgtt
     7741 ttttcttcct gtacctgtct gtgttgttca ggctctacgg cctttagtca gtctatgata
     7801 cgacgaatct gtatcgggac tcatcgatat taggtaacaa tacgcgg
//
LOCUS       DAZPEU010000173         7788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPEU010000173 DAZPEU010000000
VERSION     DAZPEU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.174"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            181..399
                     /locus_tag="ACHU2L_003344"
     CDS             181..399
                     /locus_tag="ACHU2L_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR1358071.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            617..859
                     /gene="yiiF"
                     /locus_tag="ACHU2L_003345"
     CDS             617..859
                     /gene="yiiF"
                     /locus_tag="ACHU2L_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR1358072.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1189..2118)
                     /gene="fdhE"
                     /locus_tag="ACHU2L_003346"
     CDS             complement(1189..2118)
                     /gene="fdhE"
                     /locus_tag="ACHU2L_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR1358073.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2115..2750)
                     /gene="fdoI"
                     /locus_tag="ACHU2L_003347"
     CDS             complement(2115..2750)
                     /gene="fdoI"
                     /locus_tag="ACHU2L_003347"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1358074.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2747..3649)
                     /gene="fdoH"
                     /locus_tag="ACHU2L_003348"
     CDS             complement(2747..3649)
                     /gene="fdoH"
                     /locus_tag="ACHU2L_003348"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR1358075.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3662..6712)
                     /gene="fdnG"
                     /locus_tag="ACHU2L_003349"
     CDS             complement(3662..6712)
                     /gene="fdnG"
                     /locus_tag="ACHU2L_003349"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6125..6127),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1358076.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6906..7739
                     /gene="fdhD"
                     /locus_tag="ACHU2L_003350"
     CDS             6906..7739
                     /gene="fdhD"
                     /locus_tag="ACHU2L_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR1358077.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHVAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1800 a         2006 c         2075 g         1907 t
ORIGIN      
        1 atcctgggcg ataactaaac catttttgag taataaatga aacaactgca acgctcttat
       61 tatttctgga aaacgcctcg caaaaacaaa atgacatgca aaaatccgca tttttttaaa
      121 tgcttttccg gcgtaatttt cgcacataca ttaatgagta atatatgtaa ggtggtggta
      181 atggcgatga atacggtttt tcttcattta tcagaagagg caattaaacg gctgaacaag
      241 cttcggggct ggcgtaaggt ttcacgatct gcaattttac gcgaagcggt tgagcaatat
      301 ctggagcggc agcaatttcc ggtgcgtaaa gcaaagggag gcaggcaaaa gggggaggta
      361 gttggtgttg atgatcagtg taaggagcat aaggaatgaa aaaaattttt cattcttgaa
      421 tataaaaaac agatgccctt attctggtat taatacaagg ctgttttact tgaacttata
      481 ataactgcaa ctgttacatc gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt
      541 aaatgagctt tctttaaaga ttcggcaagg gtacttttct ctccatagat tcaaaaatga
      601 tattggcgag atatttatga actctctggc aggtatagat atgggcagaa ttttactcga
      661 tttatcgaat gaggtgatta agcaacttga tgatctcgag gtgcagcgta atcttcctcg
      721 tgcggaccta ttaagggaag cggtagatca atacctgata aatcaatcgc aaacagcaag
      781 aaccagtgtt cctggcatct ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa
      841 gctgcgcgag gaatggtaat gggaaaaaat gatgtcaatc agattgctga caacgtgcgc
      901 gttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaagtca tgcaaattca
      961 atatattgaa gtttattggt aggcctgata agcgcagcgc atcaggcaat catacgcttg
     1021 tcatcagtga catacccata accttatcga tgtgttggag ggaagaacga ggcaaacaga
     1081 caattgcggc atgatcttga atgggtaacg cggaatacga gagatggtgt cgtcacaccc
     1141 tatgagctgt aatccgggcg acctgcgcca cccggaaaac gtcagagatt actctccttc
     1201 acccggaaac aggaacgggt taatggaact acgggcgtag ccttcttgct ccattcgcgc
     1261 gtccagtacc agagaggcga ggtcatctgc cacggcttca actttcggtt ctttttcctg
     1321 ataaagaatc ttcaggtaag tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg
     1381 ttcgtcatcc agcgaccagt aatgcagttt gccgctctgc tcgcagttgc tgcattttac
     1441 gcgcactacg tgccattcgg tttcacacag gttgcagtgc aggtaacgca gaccctgagt
     1501 ggtgccaatt tgcaccatgc tggacaccgg catagagcca cataccgggc aatattgacg
     1561 ttgttcgccg tattcagcgc gggctttgcc ggggatcaga ttggccatct gcgcccagta
     1621 gagcgacagc gcagcccaga taaacggcgc tttatcgctg ctgacggacg agaaatcaga
     1681 ggcaaacagc gcgctggcca tatcttccag ctcctgagtc gatgccttct ccagattctc
     1741 aatcactgcc agcgccgggc cgctcatttc aggtttcagc tcagcaatta gcgccatcag
     1801 cagcttttgc cagtgcttat cacgcggcag aacgtgaata tccagcgggg gcttgccctg
     1861 tgcgctggct tctttaatgc gcgcggtcag atccatctcc agcggatggt cgtacagcac
     1921 cactttctgg gcgtgggcga taagcgcagc aaagcgcagg taatcaccca gcggattatt
     1981 ttctgccagc tcgcgcagac gctcggcgcg gcggttgtat aaattcttga gccgagggaa
     2041 caataacggc ggaatcatat ccgccgtacg tttctcgctc gaacccagct catcttgcgg
     2101 gattatgcga atactcattc agctttcttt tccgttgtct tgcggacctc acggtaccag
     2161 cgcgggtgat gtttcttcgc ccaggcgctg gtaacccatc cttccaccat ggcggtaatc
     2221 gtgcctttca cccaaagggc ggcgtagata tgcaccatga taaccacaat taacgccact
     2281 gcggcaaatg aatgcagcat taacgcgaat cggatcaccg ggattgagaa agcaggcgca
     2341 aaataaggac gccagataat cacgccgctc accagcagca gaaccaggaa aataatcgcc
     2401 gcccagaaaa cgcatttctg accgaagtta taacgcccgg tgtcacctac ttcctcgttg
     2461 acgacgatct tacgaatatt cttcgcccaa aagatatcat cccgattgat taggttttga
     2521 tgccagtaac ggaaaaacat gatgatgaac gaggcaaaca taaccacgcc gacaaacggg
     2581 tgcagaattc gcgccagctg cggtgtgccc atgatttgca tcaaccagtt gaaggacggg
     2641 aacaaaaagc ccagcccgct caccgccgcc aggatgaagc agaaggcggt gatccagtgg
     2701 ttgatacgtt ccggcgcggt gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct
     2761 cgtctttctc ttcgtgcaga ttattctctt cctcatccgc acggttcgga ccgacaccca
     2821 cgtagtggaa gatactggcc gcaaaggtag ccgcaaagcc aacagccgcg agcggtttcc
     2881 agatgccttt ccagaatttc acggtttcgc tgatttccgg gttctccggc aagccatgat
     2941 acagatttgg cttgtcagca tggtgcagca cgtacatgac gtgtgtacca ccgacgcctg
     3001 ccggatcgta cagacccgca ttgtcgtaac cgcgggtttt cagctcagca acgcgctcgc
     3061 tcgccagcgt tttcatcgac tctttcgtgc cgaagtgaat cgcgcccgtt gggcaggtct
     3121 tcacgcaggc tggttcttgc ccaaccacca cgcggtcaac gcacagcgta catttgtaga
     3181 cgcggttgtc ttccgggttg aggcgcggaa tgtcgaacgg acagcccgca atgcaataac
     3241 cgcagccaat gcactgctcg gactggaagt cgacgatacc gttggcatac tgaataattg
     3301 caccttccgc cgggcacgct ttcaggcagc ctggatcgga acagtgcata cagccgtctt
     3361 tgcggatcag ccattccagt ttgtcgttct gctccacttc cgagaagcgc attaccgtcc
     3421 acgatttggc gcttaaatca ttggggttgt cgtacacccc aatgttattg ccgacggtat
     3481 cgcgaatgtc gttccactct gaacacgcca cctgacaggc tttacagccg atacaggtgg
     3541 taacgtcgat gagtttcgcc acttcttcct ggaagtcccg cgcctgaggc gcgggggtca
     3601 gaccgttagt cgcggaacga cgaatgatat cttgagattg ataagccata tgtcgtctcc
     3661 gttacacctt ttccacattc acaaggaagg acttaaactc cggcgtctgc gtgttcgcat
     3721 caccaacgaa tggcgttaaa gtattggcaa taaagccttt tttagctacg ccttcatagc
     3781 cccagtgaat cggaataccg atggtatcga tatctttgcc gttcgctttc agcgtgcgaa
     3841 tacgtttggt caccaccgct ttggctttga tatagccacg gttggaggag actttcacgg
     3901 tatcgccctg ggcaatgcca agtttattcg ccagcgactc cccgatttcc acaaactgct
     3961 ctggctgcaa aatcgcgttc aacagcgcgt gtttggtcca gtagtggaag tgctcggtca
     4021 gacgataggt ggttccgaca tacgggaact tatcggcttt acccaatgct tcggcgtcgt
     4081 ctttaaagat acgtgcagct gggttcgaga taacgtttgg atgcagcggg ttagttccca
     4141 gcggcgtttc aaacggctcg tagtgttccg ggaacggacc ttccgccatc ttatcgaggg
     4201 caaacagacg ccccatgcct tcctgctgca tgataaacgg cccgacgccg ctacccggag
     4261 gcgctgcgct gtagtccgga atatcccagc cggtccactt agtgccgtcc catttcagca
     4321 actgacgctt cggatcccac gggttaccct gcggatctgc ggaggcgcgg ttatacagaa
     4381 tgcggcggtt aagcggccat gcccatgccc agcccagcgt gttaccgagg ccagacggat
     4441 cggcgttatc acgacgcgcc atctggttgc cttccggcgt ccagctaccg gcgaaaatcc
     4501 agcagccaca ggaggttgta ccgtcatcgc gcaattgggc gaacgagcta agttgttggc
     4561 ctttcttgac gataaccgca ccggttgccg gatcggtaat atcggccagc gccttaccgt
     4621 tgctctccat cgctacttct tccgacttcg gctcatgcgg aatggcgtag ttccaggtca
     4681 tgttcagcac ctgatccggg ttcgcgccac cctgttcggc atacatcttg cgcaagcgca
     4741 ggaagatacc ggagaggatc tcaccgtcag tcagcgcaat ccccggggcg tccgcacctt
     4801 tccagtgcca ctgcaaccag cgacctgagt taacgattga accgttctct tctgcgaagc
     4861 aggtcgatgg cagacggaac acttcggtct ggatcttcga cgagtcaact tcgttcagct
     4921 caccgtggtt ctgccagaag ttagaggttt cagtgttcag tgggtcgatg gtgacgagga
     4981 acttcagttt cgacagacaa ccgatcactt tgtttttgtt cgggaatgag gcaacagggt
     5041 taaagccctg gcagatatag ccattgacct tgccctcttt catcatctcg aagtattgca
     5101 ggacgtcgta gcctttatcc cacttcggca accagtcaaa gccccagcta ttttccgccg
     5161 tcgctttatc accaaagaag gccttcatca tagagacgaa gaatttcggg tagttgcccc
     5221 agtagtttac ctggccttcc agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca
     5281 gatcggtctg cttctcgctt ggcagcgtca tgtaacctgg cagactctgc gacagtagcc
     5341 ccaggtccgt cagcccctga atattggagt gaccgcgcag ggcgttaacg ccgccgccgg
     5401 ccatccccat attgccgagc agcagctgga tcatcgccat cgtacgaatg ttttgcgcac
     5461 caacggagtg ttgcgtccag ccgagggcat acaggaacga ggcagtttta tcgtgagcac
     5521 tggtttctgc gatgtattcg cagactttca ggaacgcgtc ttttggcgta ccacagatgt
     5581 tttcaaccac atctggcgtg tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc
     5641 gcggatgttg cagcgtggta tcgcgtttgg cgaagccgtt ttcgtccagt tcataagtcc
     5701 aggaggattt atcgtacttg cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa
     5761 agccgtaatc ctcacgcacg atcaggctgg cgttggtata ggcttcggtg tattcgtggt
     5821 tgaatttttc attgttcagc aggtacagca atacgcctga caggaaagca atgtcagtac
     5881 cggaacgaat aggggcatag tagtcagcta ccgccgccgt acgcgtaaag cgaggatcga
     5941 tcacaatcag cttcgcgccg ttgtgaattt tggcttccat cgcccagcgg aacccgaccg
     6001 ggtgagcttc agcggcgtta ccgcccatca ccacgacgag gttggcgttc ttgatgtcga
     6061 cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc aagacttgct accgttggtc
     6121 cgtgtcagac acgcgcctgg ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt
     6181 gcgttaaata gccggtttcg ttactcgacg cggaagcaca cagcatcccg gtggagagcc
     6241 agcggttaac agtcacgcct tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt
     6301 tcatcagttt ggcgatgcga tcaaacgcct cttcccaact gatttgctgc catttatcag
     6361 aacctggcgc acggtattcc ggaaacttca gacggctttc ggagtggatg aaatccacca
     6421 ggccagcgcc tttcggacaa agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga
     6481 tatggaagat agatgctttg gcgttttttg ctccgtcacc gaggctgtac atcaacagcc
     6541 cacagcctac ggaacaatag gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat
     6601 actgccgggt ttccgcgagt gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc
     6661 ctgccatacc gccagcgcag atcttaaaga actgccttct gctgacctgc atggattgct
     6721 ccttgtttcg acattgtcac gtcccattta cattcgcttg ctgcgtgtgc agggagtggg
     6781 agttattttt ctttgcggaa ggggccgcaa aggtccagaa ttggctcaat ttccctccat
     6841 ctaggaaggg tttgtaacag aataccataa tgttggtgtg tatgttctta tctggttaag
     6901 agaaagtgaa aaaaacacag cgaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc
     6961 gtcaaattga gttatggcgg cgtgatgatt tacaacaccc acggttagat gaggtcgcgg
     7021 aagaagttcc cgttgcgctg gtctacaacg gcatttcgca tgtggtgatg atggcgtcgc
     7081 ccaaagacct tgagtacttt gcgctcggtt tttcgctttc cgaggggata atagaaagtc
     7141 cgcgcgatat cttcggcatg gatgtcgttc cttcctgtaa tggtcttgaa gtacaaattg
     7201 agctttccag ccgccgcttt atggggttga aggagcgccg ccgggcgctg gcgggacgta
     7261 cgggctgtgg cgtatgcggc gtggagcaac ttaatgacat cggaaaaccg gtgcagccgc
     7321 taccgttcac ccagacgttt gatctcaaca aactggatga tgcattacgt catctcaacg
     7381 attttcagcc agtggggcga ctgactggtt gtactcacgt cgctgcctgg atgttgccat
     7441 ctggcgaact ggtcggcggg catgaagacg tgggtcgcca tgtggcgctg gacaaactgt
     7501 taggccgccg gtcacaagaa ggggaaagct ggcagcaagg tgcggtactg gtttccagtc
     7561 gtgccagtta tgaaatggtg caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg
     7621 tgtctgccgc gaccacgctt gctgtagaag tggctgagcg ctgtaatctg acactggtag
     7681 gtttttgtaa accgggtcgg gcaacggttt atacccatcc gcagcgtttg agcaattaat
     7741 taaaataagt cctgcgatat atattaaatt gcaggactta ttcatttc
//
LOCUS       DAZPEU010000174         7746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPEU010000174 DAZPEU010000000
VERSION     DAZPEU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.175"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACHU2L_003351"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACHU2L_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR1358078.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="ACHU2L_003352"
     CDS             1104..2564
                     /locus_tag="ACHU2L_003352"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1358079.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACHU2L_003353"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACHU2L_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR1358080.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACHU2L_003354"
                     /gene_synonym="ydfH"
     CDS             complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACHU2L_003354"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR1358081.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACHU2L_003355"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACHU2L_003355"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR1358082.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="ACHU2L_003356"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="ACHU2L_003356"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR1358083.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="ACHU2L_003357"
     CDS             complement(6729..7247)
                     /locus_tag="ACHU2L_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR1358084.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7746
                     /gene="ydeI"
                     /locus_tag="ACHU2L_003358"
     CDS             7523..>7746
                     /gene="ydeI"
                     /locus_tag="ACHU2L_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR1358085.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1941 a         1813 c         1957 g         2035 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca
     7741 caaagg
//
LOCUS       DAZPEU010000175         7721 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPEU010000175 DAZPEU010000000
VERSION     DAZPEU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.176"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..832)
                     /locus_tag="ACHU2L_003359"
     CDS             complement(<1..832)
                     /locus_tag="ACHU2L_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1358086.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="ACHU2L_003360"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="ACHU2L_003360"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR1358087.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACHU2L_003361"
     CDS             complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACHU2L_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR1358088.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACHU2L_003362"
     CDS             complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACHU2L_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR1358089.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACHU2L_003363"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACHU2L_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR1358090.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACHU2L_003364"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACHU2L_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR1358091.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="ACHU2L_003365"
     CDS             complement(5530..6513)
                     /locus_tag="ACHU2L_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1358092.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="ACHU2L_003366"
     CDS             complement(6559..7383)
                     /locus_tag="ACHU2L_003366"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1358093.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACHU2L_003367"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACHU2L_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR1358094.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2012 c         1812 g         1830 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac t
//
LOCUS       DAZPEU010000176         7596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPEU010000176 DAZPEU010000000
VERSION     DAZPEU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.177"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(119..1590)
                     /gene="yeeO"
                     /locus_tag="ACHU2L_003368"
                     /pseudo
     CDS             complement(119..1590)
                     /gene="yeeO"
                     /locus_tag="ACHU2L_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1751..1826
                     /locus_tag="ACHU2L_003369"
     tRNA            1751..1826
                     /locus_tag="ACHU2L_003369"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1784..1786,aa:Asn,seq:gtt)
     gene            complement(1864..2814)
                     /gene="cbl"
                     /locus_tag="ACHU2L_003370"
     CDS             complement(1864..2814)
                     /gene="cbl"
                     /locus_tag="ACHU2L_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR1358095.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2916..3833)
                     /gene="nac"
                     /locus_tag="ACHU2L_003371"
     CDS             complement(2916..3833)
                     /gene="nac"
                     /locus_tag="ACHU2L_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR1358096.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4161..4236
                     /locus_tag="ACHU2L_003372"
     tRNA            4161..4236
                     /locus_tag="ACHU2L_003372"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4194..4196,aa:Asn,seq:gtt)
     gene            complement(4292..5227)
                     /gene="ldtA"
                     /locus_tag="ACHU2L_003373"
     CDS             complement(4292..5227)
                     /gene="ldtA"
                     /locus_tag="ACHU2L_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1358097.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5289..6368)
                     /gene="cobT"
                     /locus_tag="ACHU2L_003374"
     CDS             complement(5289..6368)
                     /gene="cobT"
                     /locus_tag="ACHU2L_003374"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR1358098.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6380..7123)
                     /gene="cobS"
                     /locus_tag="ACHU2L_003375"
     CDS             complement(6380..7123)
                     /gene="cobS"
                     /locus_tag="ACHU2L_003375"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR1358099.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7120..>7596)
                     /gene="cobU"
                     /locus_tag="ACHU2L_003376"
     CDS             complement(7120..>7596)
                     /gene="cobU"
                     /locus_tag="ACHU2L_003376"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR1358100.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2098 a         2054 c         1648 g         1796 t
ORIGIN      
        1 cgactgaact acagaggaat cgtgagaacg aggcgaatat tagcgatgcc cacccacaat
       61 gtcaaagcct gttttttaaa tttgaaatcg tttgctgaaa taatctgcat tttgtcgttt
      121 attccgacac aactggcttt ttttcacact tttgcggctc ggatcgaggg tatttccaca
      181 gccaacgtcc agtaaccatt cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa
      241 acattcccat ccagacacca accacacccc agccaagcat gattccctgc acataaccga
      301 ctacaacccg gcaaccccac atgctcaaca tagaaaccca catggcgtaa cgggcatcac
      361 gagcaccctt aaatccagcg ggtagcaccc atgaggcgga ccaaataggc ataaataaag
      421 catttagcta aatcagaatc acaacgacgt gtttaacctg tggatcctgg gtgtaaaacg
      481 atgccataac cccggcaaag ggagccgtta gccaggcgat ggccgttaat ccaagagtgg
      541 acagccagaa cacatgccgc aactgaatct ctgcttgcgc tatctgccct acccccaacc
      601 ttcggcctgt aatgatcgta gaagcagaac cgagcgcact tccgggtaag ttgataagag
      661 ccgcaattga gaacgcgata aaatttccgg caataacgct ggtccccatc ccggcaacga
      721 acatttgggt taataaccga ccactggtaa ataacactga ttcgacactc gctggaatac
      781 caatccccat gacttcccag ataatgctaa aattcagcgg tttaaaatag ctctttaacg
      841 aaatccttag cgcaggatta aaaccaatcg ccagcaccca caaaattgca actgcgccaa
      901 tataacgaga aatggttaaa cccaacccag ccccgaccag gagaaaaggc cgtaaatcaa
      961 tatgccgcta ataataatat taagaatatt caggctaccg ttaatcagta gcggtatttt
     1021 cgtattccct gcaccacgaa gtgccccgct accaataaga gtgatggcag cggctggata
     1081 actgagtacg gtcagctcca gataagtcaa cgccagtgct ttaacttctg tcgtggcatc
     1141 acccgcgacg aaatcaataa tttgttcgcc aaaatgatga attagcgttg ccaacagtac
     1201 ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc gtcgtcgatc
     1261 ccgcttaccg agactaaatg ctacaacgac agtagtacca agatcgatag ctgcaaaaaa
     1321 agccataatg accatattga agctgtccgc caaccccacg ccggccatcg catcttttcc
     1381 cagccaactg accagaaaag tgctcaggac ccccatcaac aggacacagg cgttctccat
     1441 gaagatagga acagcaagcg gggttatctc gcgctagaac aacactttgt agctcttgcg
     1501 tttagagtgc cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga tattcaaagc
     1561 cgaccttaat tgcagaaagt gaaaccacat ttcaaatgat gagggagaat cagcaaagct
     1621 gcaaagattt tcgccaacaa attgtctgca aatgcaacaa actgttgata gaaacggcaa
     1681 acagttggga aatttaaaaa tcgggtttga caaaatattt ttcgccgtta agatgtgcct
     1741 caacagcgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac
     1801 tggttcgagt ccagtcagag gagccaaatt tctgaaaagc ccgcttttat agcgggcttt
     1861 ttgctatatc tgataatcaa tttcctcttc actgttttcc atcacctggc gcttgatatc
     1921 ctcaactgac agtcctgcat tacaaagttc cagaaagcgc caaacatagt tacgctgaag
     1981 ttgtcctcgc ttcagtccca accagacagt attagcatcg aaaagatgcc gcgtatccag
     2041 gcggattaaa ttctcttcct cttgttcgcc gctggattgt tcggcaacta atccgatccc
     2101 aagcccaaga gcaacatagg ttttaatgac atcagaatcc tgtgcactta atacaatatc
     2161 tgccggcaaa cctttgcggg taaatgcgtc atcagtgcgt gagcgccccg taattccctg
     2221 tcggtaagta attaacggcc acttcgctat tgattccagc gtcaatggtg taatttgcgt
     2281 taagggatga tcgagtggaa caagcaaact atggtgccaa caaaaccatg ggaaggcgac
     2341 gagctgcggg tcattactca aacgctcgct ggcgatacca atatcagctt cgccattttg
     2401 caacaacgtc gcaatttcct gtggcgaacc ctggattagc tcgagccgaa cctccgggaa
     2461 aagttcgcga aaagctttaa tgacctctgg caagctataa cgtgcctgag tatgcgtcgt
     2521 tgcaatagtg agaacaccag acgtatcgtt ggtaaacagg tctgcaagcc gacgaacatt
     2581 actggcttca ttcagaatac gttctgcaat gaccagtaat gctttgcccg gttcagtcat
     2641 ccccagcagt cgcttacctc gtcgaacaaa tatttcgatg ccaaattcat cctccagttc
     2701 ccgaatatga cggctaacgc ctgactgtga ggtaaaaagc atattcgcaa cctctgtcag
     2761 gttgtaatcc tgacgtgcag cctcgcggat tatctttagt tgttggaaat tcacggtaaa
     2821 ctccgggcag ttcagatatc ccgttattgt taaagtctaa tgcccggcat aacaaataat
     2881 aaaaacccgc atcttattcc atcccgatat aacgcttagc tcaccaattg ccactgcctt
     2941 ttttccatca ctggagaact aatcactgac attaacaact ctttcactgc ctgtgcctgt
     3001 ggtgacaagt ttgctctggc gggtaaattt aaggacaaag agagactcat tgaaggagtg
     3061 gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcagc cgattcaggt
     3121 aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat agactcaatt
     3181 tcaccaatga cttttgccgt gagccgccgt agggaaaaag cctcatcaac acgaagtcta
     3241 acagcgctgt aatcactggg gagaaaaagg ttcatttgcg caatagcatt aacatcaacg
     3301 ttttgccccg ggcaatcttg agttcctacc aggaaaagat cttctttcag caaagcctga
     3361 ctggataccc cagccacagg ggaatgctca taaatcaccg ccatatcgag ttggtgattt
     3421 atcagttttt cgttaagcac tgcaccacta ttttcatgaa gatagataac gacctccggg
     3481 aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc ggttcctggc
     3541 gcaaagccaa tcgagacttg ccccgataat gactgaccaa cgttatgcac cgccagttgg
     3601 gcctgctcac actgacgtaa aatggcccgt gcatgggtat agagaatttt tccggcgtct
     3661 gttggcgtaa cgccccgttt tgtgcgaatc aaaagctgtt gatttaactc accttccagt
     3721 atggcaacct gttggctgag cgctggttgt gcgatatgca atacttcagc agcctgggtc
     3781 aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt catgttgcct
     3841 ccggttttta agaatcggcc caagtgccgc cattacttac aaccagtatg caagatgcct
     3901 gccagttcta ttttggcgtt gatgcgcaag ctaaccaact gtcaaataag agattatgat
     3961 agattcgtca ttggcccttt ttatcagcag tcgcattcac ctgccctata aaaggagggt
     4021 atgcaccacg atggttcatt acccaataag attgaaagct caccactttg ttgaaattgg
     4081 cagcaaacaa acaaaaaaaa tgcatttcac cctttgacat caccatgcac tgccattaat
     4141 atgcgccccg ttcacacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     4201 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt caaaaaagcc tgctttcgag
     4261 caggcttttt actttctaat taccaacgct cttaaaacat ctgtcttgaa ccagaactaa
     4321 tttgcacagg cattcccgat cgatgttgca acgcagcatt tgcgcgattt acatcaactt
     4381 cttgcccgtt gataaacgcc cgcaaagatg gggttaccgg caatggcact tttcggtcag
     4441 actcatattc tgcacgattg cgcgacagcg gctcatgaac ttccagccac tttgaaccat
     4501 ccggttccgt tgtgtatttc actggctgat caataatttg tacacgagtg cctacaggaa
     4561 cattatcaaa cagatatttg atatcgtcat tacgcagacg aatacagccc tgacttacac
     4621 ggagcccaat accaaaattg gcattggtac cgtggatggc atacaacctg ccaatataaa
     4681 tcgcgtacag ccccatgggg ttatcgggcc ccgcaggaac aaatgcgggc aaactctccc
     4741 ctcgtttcgc atattcgcgc cggatgttcg gcgttggcgt ccaggttggt gcttcttgtt
     4801 tacgttcaac ggtagtcacc cagttacgcg gggtttctcg cccagcctgg ccgataccaa
     4861 taggaaagac ttccactgta ttactgtctg gcggatagta ataaagacgc atttcagcga
     4921 cgttaaccac aatcccttta cgaacagtgg cgggcaaaat cagttgctgc ggaatggtga
     4981 gttgtgagcc aggcttcggc aaaaaaacat cagcgcccgg attcgcttcc agcatattac
     5041 ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgagtattg tgatcaggaa
     5101 cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat ggataagtta
     5161 ccgccaggct attttggctg gcaaaaagca gagcaaatga gcaaagaata tttacacgac
     5221 gcatcatgtc cctttcctaa gtcgcgaata ctatccgata agtatagctt ttatcagact
     5281 tttcgttttt agctgttcaa atcagaagtc gtattccccg gtagaacaat attactggca
     5341 gcaagttcgc ccatgttgtt gtatatcgca caagcagctt cgatgatgga catcgccaga
     5401 gctgcaccac tcccctcacc taaacgcata tccatattga gataaggctc cagccccaaa
     5461 tgcgagagcg ctatacgcgc gcctttttct gccgacaagt gagaaggaat gagatacggt
     5521 ttgattgcag gagacatctg gcaggctgcg agcgcagcag cataagaaag aaatccatcc
     5581 agcaacacgg gtaaaccgca ggaagcagca cctaacatca ctccagctat tccgaccaaa
     5641 tcaaatccac ccacttttgc caggacatca acaccatcct gaggatttgg ttgattcaac
     5701 gtaatcgccc gacgcacaac atcaatttta ttagccagtt tatctgtcgg caggtttgcg
     5761 ccaatcccaa ccacttcttc aggatcccgg ccagtgattg tgctgactat tgccgctgcc
     5821 ggtgtcgtgt ttgccatccc cagttcacct acaccaaaca gcgtgacacc gtttttcgcc
     5881 agctcccgcg tataacatat gacgtccaaa agcaactttt cagcctgacg gcgactcatt
     5941 gccggagctg aagcaatatt gccgctacct cgtgcgacac gcatgttgat aagcccgggg
     6001 ataggctcag cagtatcaat accaacatca attacgtgga cgttagcgcc cgcttgtgct
     6061 gccagcacac acacgccggt tgttccacgg gtcatatttt cagcctgtat ggctgtcact
     6121 tcttttggag aaatagcgac cccttcctcc cagacgccgt gatcggcaca cataaccagt
     6181 accgcttttt tgcccacatg cggtatgcca ttcacccccg gcattcctgc cagttgtatg
     6241 gcaagcgcct ccagctttcc taggctacca acaggtttga ataacccgtc aacatgccgt
     6301 tgtgcacgcg acatagcggc agaattgatg gctgggatcg tattcagtaa atcggcaatt
     6361 atttgcatgt cacgtttctt tatagcagtg ccagcaggaa gaccagttca ccaagctcta
     6421 tggctgctcc cagcgtatcg cccgtttgcc cgcccagcgt acgtttgaga agctgaccga
     6481 ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata cccagcaata
     6541 atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc ccatcaattt
     6601 tgccaatgaa cacattgcca agcccttctt cccgcgcata acgatgacga tacatcaata
     6661 atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg atcggttcgc
     6721 cacgcagagc caactcgctt aacaccagaa tctttgccag taccacaaaa atcaatgcca
     6781 gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga tcgcgactac
     6841 gtgcagaaaa tacgccatca caggtatcag cgaggccgtc cagatggaat tccccggtca
     6901 tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tgcgccacac catgcctgca
     6961 gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaatcaaa ggaaaagtaa
     7021 taataccgcg agaataatgt tcgaaatcca gtccctggga ccagcgacgc ggtacaggca
     7081 gacgcgtaat aaatgagagc atcgcccaaa ataatttgct catttgattt ttactccaat
     7141 acccgaaacc accagccata cttcatttgc cgcagcggcc aactgctgat ttacccgccc
     7201 ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca ttcccacttc
     7261 gttagtcact aatacaacct ttgcggggca acgttggcag gcagcaatca acgactgaat
     7321 ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt ctttatcgcc
     7381 gccataatca aacaacagat tagtcaccat tgtggtaacg cattccagca acacagcctc
     7441 attcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct ccactgtgcg
     7501 ccagtgctcc gggcggcttt gccgatgatg ttctatccgt gcagccatct catcatcaag
     7561 gatttgcgag gtagcgatat acagaacctg tgaaga
//
LOCUS       DAZPEU010000177         7552 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPEU010000177 DAZPEU010000000
VERSION     DAZPEU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.178"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(316..1716)
                     /gene="asnS"
                     /locus_tag="ACHU2L_003377"
     CDS             complement(316..1716)
                     /gene="asnS"
                     /locus_tag="ACHU2L_003377"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR1358101.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1885..3087)
                     /gene="pncB"
                     /locus_tag="ACHU2L_003378"
     CDS             complement(1885..3087)
                     /gene="pncB"
                     /locus_tag="ACHU2L_003378"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR1358102.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3353..5968
                     /gene="pepN"
                     /locus_tag="ACHU2L_003379"
     CDS             3353..5968
                     /gene="pepN"
                     /locus_tag="ACHU2L_003379"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR1358103.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(6175..6942)
                     /gene="ssuB"
                     /locus_tag="ACHU2L_003380"
     CDS             complement(6175..6942)
                     /gene="ssuB"
                     /locus_tag="ACHU2L_003380"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1358104.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6939..>7552)
                     /gene="ssuC"
                     /locus_tag="ACHU2L_003381"
     CDS             complement(6939..>7552)
                     /gene="ssuC"
                     /locus_tag="ACHU2L_003381"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR1358105.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         1923 a         2001 c         1909 g         1719 t
ORIGIN      
        1 atgatttact tatatttaaa ttaactaaac ggaaattttg tttctgatgg aaactttatc
       61 gacctggcac aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt
      121 gctatcagct aagaaaaaac taatccgcaa taagagtacg ggttattttt tgtgcgtaaa
      181 tgttgcaatg taatattccg taacaggatg atcgttatgg cgggggtgca aatagattgc
      241 ttgacttaac aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct
      301 ctttgaggaa gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc
      361 acgcacgttt tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc
      421 tgaatgcgga acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt
      481 caggcccatt tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc
      541 accaatgatc tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc
      601 ttcgttgagg cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg
      661 tgctttaaag tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca
      721 gtaaaccggg ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta
      781 atccacctgc gcgaaatcgg cttcaatgaa gcgttccaga cgtgaaacgg catctttatc
      841 tacgcgttca gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac
      901 atatttcagc atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc
      961 cggctccagc atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa
     1021 agtcgggccg aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg
     1081 gccagatacg gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc
     1141 ctgatcgtta cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc
     1201 accttcggta tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt
     1261 aaagaagcga tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag
     1321 gtttgtgcgc ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc
     1381 cgccatcggg taagtgtctg gatcttcaac ccaaccagca acttcaacct tgctggcctg
     1441 aatttcaaat tgttgcccct ggcccggcga tgccacgact ttacccgtca caatgaccga
     1501 gcagccggtg gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac
     1561 agcctgtaca ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt
     1621 tgaatctcgg cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg
     1681 gccctggagt acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg
     1741 aaaaaataaa cacttgtcca cccgtaaatg ggggggatac ctatgttacc tggcatctgc
     1801 aatcagacaa gcagaattcg caaatgcagc gaaagatttc ggaattaaag gtaaaaaagg
     1861 gagccgatta gctccctgat gatattaact ggctttttta atatgcggaa ggtcgaacgc
     1921 tttgcgcagc gcccgaacaa acgctttatc atggcagata gttttgccag ggctgtcaga
     1981 aagtttcgcc accggtttac cgttacactc taccaactta atcacaatat tcaggggttt
     2041 tacctggggg atatcgcagg tcaggcgagt cccaatacca aaacttaatt gcacgcggga
     2101 agagaagtgg cggtatagct caaccgcttt gcgtaaatcc agattgtcag agaaaaccag
     2161 cgttttactc tgtggatcaa ttcccagctt ttcataatgt gcaatggctt tttcacccca
     2221 ttcaaccggg tcgccagagt catgacgcag gccctgatac cgactagcga actcgacacc
     2281 gaaatcacgc aggaaagcat ccatagtgat gcagtcggtt aatgcaatgc caagttggtc
     2341 gggatactct tccagccagg cagcaagtgc agctcgctgg ctgttggcta gatccgggct
     2401 gatttgctga tgtgcctgga accattcgtg tgcctgtgtt cccatcggcg tgagggaaag
     2461 ccgacgcgcc agatcgtagt tgctggtgcc cacgaaccag gattcctgtt gcagacgctt
     2521 aacgatggtt tcttgtactt cgcgagaaaa acggcgacgg gtgccaaaat ccatcagatg
     2581 gaagcgcgac atatcaagac cggcggttaa cgccgagaag tcgactaatt tgctttccag
     2641 cgtgtcgagg gcttgcgcaa cgtcggcctg cggtgagcga tagcgatgta ccatttcact
     2701 gataaccgcc agcaaaggaa cttcccagag gatgacttca cgccacggac cgcttaagcg
     2761 aatatccagc ttgccattat cgttggacac ggtgacttgt tccgggttaa agcggaactc
     2821 gcgtaaccag ttaagatagt cggccttaaa gaaaggcagg gcagagagcc actgatattc
     2881 atcatcctgc aggcgcaggt gctgcatcgc ctgaatctgt tcacgaatag catcggcata
     2941 aatacccagc agatcgtcac ctcggcaacg aaactccgcc gcgacatgca catcgtaata
     3001 gtgatgaaac acggcttgct gcatatgcaa cttataagca tctgtatcca gcaacgagtg
     3061 cagaacagga gaagcgaatt gtgtcatagg tgcgcagtag cgtcctcgta caggagcgtt
     3121 tagtacaata aacatcttca ggaactgctg gagtatacct tgtttcgcaa tttattgaac
     3181 cccgatcaca ccatatgcca cctttctggt cgatggcatt acgcggtgta tgttataaaa
     3241 atgtagcaat aaaggcgttt gtacctgaaa agatgaagat tctgcatagc gcgatttacg
     3301 caacaggaat agactgaaca ccagactcta taaaagatgc taaaggttat ttatgactca
     3361 acagccacaa gccaaatacc gtcacgatta tcgtgcgccg gattaccaga ttactgatat
     3421 tgacttgacc tttgacctcg acgcgcaaaa gacggtcgtt accgcggtca gccaggctgt
     3481 ccgtcatggt gcatcagatg ctccgcttcg tctcaacggc gaagacctca aactggtttc
     3541 tgttcatatt aatgatgagc cgtggaccgc ctggaaagaa gaagaagagg gcgcactggt
     3601 catcagtaat ttgccggagc gttttacgct taagatcatt aatgaaataa gcctggcggc
     3661 gaatacggcg ctggaagggc tttatcagtc aggcgatgcg ctttgcaccc agtgtgaagc
     3721 cgaaggtttc cgccatatta cgtattatct cgaccgcccg gacgtgctgg cgcgttttac
     3781 caccaaaatt attgccgata aaatcaaata tcccttcctg ctttccaatg gtaaccgcgt
     3841 tgcgcaaggc gaactcgaaa acggacgcca ttgggtacag tggcaggacc cgttcccgaa
     3901 accgtgctac ctgtttgcgc tggtggcagg cgactttgat gtactgcgcg atacctttac
     3961 cacgcgttct ggtcgcgaag tagcactgga gctgtacgtc gatcgcggca accttgatcg
     4021 cgcgccgtgg gcgatgacct cgctgaaaaa ctccatgaaa tgggatgaag aacgctttgg
     4081 cctggagtat gacctcgaca tctatatgat cgtcgcggtg gatttcttca atatgggcgc
     4141 aatggagaat aagggtctga atatctttaa ctccaaatat gtgctggccc gcaccgacac
     4201 cgccaccgac aaagattacc tcgatattga acgcgttatc ggccatgaat atttccataa
     4261 ctggaccggt aaccgagtga cctgccgcga ctggttccag ctcagcctga aagaaggttt
     4321 aaccgtcttc cgcgatcagg aattcagctc tgaccttggt tcccgcgcag ttaaccgcat
     4381 caataatgtg cgcaccatgc gcggattgca gtttgcggaa gacgccagcc cgatggcgca
     4441 cccaatccgc ccggacatgg tcattgagat gaacaacttc tacaccctga ccgtttacga
     4501 gaagggcgcg gaagtgatcc gtatgatcca caccctgctg ggcgaagaaa acttccagaa
     4561 agggatgcaa ctctatttcg agcgtcatga tggtagcgca gcgacctgtg acgactttgt
     4621 gcaggcgatg gaagatgcgt cgaatgttga tctctcccat ttccgccgtt ggtacagcca
     4681 gtccggtacg ccggttgtga ccgtcaaaga cgactacaat ccggaaactg agcagtacac
     4741 cctgaccatc agccagcgca cgccagccac gccggatcag gcagaaaaac agccgctgca
     4801 tattccgttt gccatcgaat tgtatgacaa cgaaggtaaa gtgatcccgt tgcagaaagg
     4861 cggtcatccg gtgaattccg tgctgaacgt cacccaggcg gaacagacct ttgtttttga
     4921 taatgtctac ttccagccgg tgcctgcgct gctgtgcgaa ttctctgcgc cagtgaaact
     4981 ggaatataag tggagcgatc agcaactgac cttcctgatg cgtcatgcgc gtaatggttt
     5041 ctcccgttgg gatgcggcgc aaagtttgct ggcaacctac atcaagctga acgtcgcccg
     5101 tcatcagcaa gggcagccgc tgtctctgcc ggtacatgtg gctgacgctt tccgcgcggt
     5161 gctgctcgat gagaagattg atccggcgct ggcggcagaa atcctgacgc tgccttctgt
     5221 caatgaaatg gctgaactgt tcgatatcat agacccgatt gctattgccg aagtacgcga
     5281 agcactcact cgtactctgg cgactgaact ggcggatgag ctgctggcta tttacaacgc
     5341 gaattaccag agcgagtacc gtgttgagca tgaagatatt gcaaaacgca ctctgcgtaa
     5401 tgcctgcctg cgcttccttg cttttggtga aacgcatctg gctgacgtgc tggtgagcaa
     5461 gcaataccat gaagcaaaca atatgaccga tgcgctggca gcgctttctg cggcggttgc
     5521 tgcacagctg ccttgccgtg acgcgctgat gcaggagtac gacgataagt ggcatcagga
     5581 cggtctggtg atggataaat ggtttatcct gcaagccacc agcccggcgg cgaatgtgct
     5641 ggagacggtg cgtggtctgt tgcagcatcg ctcatttacc atgagcaacc cgaaccgtat
     5701 tcgttcgttg attggtgcgt ttgcgggcag caacccggca gcgttccatg ccgaagatgg
     5761 cagcggttac cagttcctgg tggaaatgct taccgacctc aacagccgta acccgcaggt
     5821 agcttcacgt ctgattgaac cgctgattcg cctgaaacgt tatgatgcca aacgtcagga
     5881 gaaaatgcgc gcggcgctgg aacagttgaa agggctggaa aatctctctg gcgatcttta
     5941 cgagaagata actaaagcac tggcttgata cttttgagcc aaaatgcctg atgcgacgct
     6001 tgacgcgtct tatcaggcct acaactccgt tgaaattgct ggttttgtag gctggataag
     6061 gcgttcacgc cgcatccggc agccgtgccc aactgcctga tgcgatgctt gacgtgtctt
     6121 atcaggccta caagtccgtt gaaaatgctg gttttgtgcg ccacatccgg catattaacc
     6181 atgacgccga atcagttgtt cagaatgccc acgccgcatt acccgttgca atacttccgc
     6241 ttccagctcc gctaatcgca ccgaccctaa tcgacgcgga cgggggatat ccactgtcaa
     6301 atccagacta atttttccct cttcaattaa caacacccgg tcagccattg ctaccgcttc
     6361 gctcacatca tgcgtcacca acagtacggt aaagccgtgc tgctgccaaa gtgacacaat
     6421 caaatcctgc atctcgagtc gcgttaaggc gtccagtgcc ccgagcggtt catcaagcaa
     6481 caataaaccc ggtcgatgaa tcaacgctct tgccagcgcc acacgctgtt tctgcccgcc
     6541 agaaagtgcg gcaggccatt ccccggcgcg attctccagt cctgccgcag ccaacgcccg
     6601 acgcgcggca tcgcgccact gacctttaag gcctaaccca acgttatcaa tcaccgattt
     6661 ccacggcagc agacgcgcat cctgaaacat cattcgcgta tcatcctgaa tttcagccag
     6721 cggtgtggtg cccgctaaca catcgcctgc ggttggcgtt tccagaccgg ccagcaggtg
     6781 cagcagggta cttttaccac caccgctgcg gcccaccacc gccacaaact gacctgccgg
     6841 aatatgtaaa tccagttggt tcaggacgat attttccgcg taatgtttgc ttactgcatt
     6901 gagcaacaat ggtgtgccct ggttcagacg agcagtattc atatcgtggc ctccttcaaa
     6961 tgataagccg ggttccagcg caaccagagg cgctctaaca gctgcgcgct gacgtcagca
     7021 agtttgccga gcagggcgta aagaataata gcgactacca ccacgtctgt ttgcaaaaac
     7081 tctcgcgcat tcatcgccag ataaccaatg ccggaattgg cagaaatggt ttcggcaaca
     7141 atcagcgtca gccacatcag gcccaacgca aaacgcacgc cgaccataat tgagggcagg
     7201 gcatcgggca ggatcacatg gataaacagc ggtatgccgg ataatccata gctacgcgcc
     7261 atctccacca gcccgcgatc gatattacgg atgccatgcc aggtgttgat ataaatgggg
     7321 aacagcgtac cgagcgccac cagaaagatt ttcgcggatt catcaatgcc aaaccacaaa
     7381 atcaccagcg ggatcagcgc cagatgcggc acgttgcgca acatctgaat tgaggtatcc
     7441 agcagccttt ctccccagcg cgacagtccg ctaatcagtc ccagaatcag gcccagcgat
     7501 ccgccaattg aaaagccaat cagcgcccgc caggaactga tcgccagatg ct
//
LOCUS       DAZPEU010000178         7536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPEU010000178 DAZPEU010000000
VERSION     DAZPEU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.179"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="ACHU2L_003382"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="ACHU2L_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHU2L_003383"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHU2L_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR1358106.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHU2L_003384"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHU2L_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHU2L_003385"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHU2L_003385"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1358107.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHU2L_003386"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHU2L_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR1358108.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHU2L_003387"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHU2L_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR1358109.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="ACHU2L_003388"
     CDS             complement(6512..7204)
                     /locus_tag="ACHU2L_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR1358110.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1916 a         1829 c         1984 g         1807 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aacatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccggg ataactccct
     7381 ataatacgcc accactgaca cggaacaacg gcaaatacgc cgccgggtca gcggggttct
     7441 cctgagaatt ccgacagaaa aagcaaaaat aaatgattga ctctgtagcg ggaaagcgta
     7501 ttatgcacac ccccccccgc gccgctgaga aaaagc
//
LOCUS       DAZPEU010000179         7526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPEU010000179 DAZPEU010000000
VERSION     DAZPEU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.180"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(77..153)
                     /locus_tag="ACHU2L_003389"
     tRNA            complement(77..153)
                     /locus_tag="ACHU2L_003389"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Arg,seq:acg)
     gene            complement(157..249)
                     /locus_tag="ACHU2L_003390"
     tRNA            complement(157..249)
                     /locus_tag="ACHU2L_003390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Ser,seq:gct)
     gene            complement(565..750)
                     /gene="csrA"
                     /locus_tag="ACHU2L_003391"
     CDS             complement(565..750)
                     /gene="csrA"
                     /locus_tag="ACHU2L_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR1358111.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(985..3615)
                     /gene="alaS"
                     /locus_tag="ACHU2L_003392"
     CDS             complement(985..3615)
                     /gene="alaS"
                     /locus_tag="ACHU2L_003392"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR1358112.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3743..4243)
                     /gene="recX"
                     /locus_tag="ACHU2L_003393"
     CDS             complement(3743..4243)
                     /gene="recX"
                     /locus_tag="ACHU2L_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR1358113.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4486..5547)
                     /gene="recA"
                     /locus_tag="ACHU2L_003394"
     CDS             complement(4486..5547)
                     /gene="recA"
                     /locus_tag="ACHU2L_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1358114.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5627..6124)
                     /gene="pncC"
                     /locus_tag="ACHU2L_003395"
     CDS             complement(5627..6124)
                     /gene="pncC"
                     /locus_tag="ACHU2L_003395"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR1358115.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6269..7354)
                     /gene="mltB"
                     /locus_tag="ACHU2L_003396"
     CDS             complement(6269..7354)
                     /gene="mltB"
                     /locus_tag="ACHU2L_003396"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR1358116.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1759 a         2108 c         1806 g         1853 t
ORIGIN      
        1 agctacggat gcatcgggaa acttacttta ctgcagattt tttgataccg ctactaaagc
       61 cgtatcaagt aagagatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta
      121 gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg ggattcgaac
      181 cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca gcctctcggc
      241 cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct catcatcgct
      301 gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc cacactttta
      361 tcgtttgcac acttcacgct caattagtct gtaaaagcgt caaaaagagt gttttatcta
      421 cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact taagttgaat
      481 gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg tgcgtctcac
      541 cgataaagat gagacgcgga aagattagta actggactgc tgggattttt cagcctggat
      601 acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta cgccaatacg
      661 tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa tcatgagggt
      721 ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt aaaagagtcg
      781 ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc aatgacaaac
      841 acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta gccgatatac
      901 acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg tgaagcgagt
      961 ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc ctttcacact
     1021 ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca tgtcaggacg
     1081 tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc ctgctttcac
     1141 acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt cggctaccgt
     1201 tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca tggtacgcaa
     1261 cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat taacatcaat
     1321 tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa gctgttgtag
     1381 ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg ccagattatt
     1441 gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg catgaacggt
     1501 ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag cagtacccga
     1561 ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag taccgccaca
     1621 cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat acttctcgcc
     1681 gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga ttcatgatgt tggtttcgat
     1741 cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa tctcttccgg
     1801 tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa ccagtgaacc
     1861 tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca ggtgcgttgc
     1921 ggagtgattc agacgaatac gggcgcgacg agcctcatca atatcagcct gcacagcgtc
     1981 gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct ggccgtattt
     2041 ctgcgtatct tccaccgcga aggagaagtt agcgcctttc agttcgcctt tatcaccaac
     2101 ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag cttcctggcc
     2161 ggcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt tgccgttcag
     2221 ttccagatgg tcatagcctt taaattcaga tgcactatca acacggatca ttgcgttgta
     2281 atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca ttgctgcttc
     2341 aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg tcaggtcaac
     2401 cgggaagcca taggtgtcgt acagacggaa agccgtttca ccatccagcg tatcaccaga
     2461 aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac gggcaaactg
     2521 ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca ggtcttcacc
     2581 cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg tttctttcgc
     2641 gccgagcata ttgccgtgac gcactgcgcg acgaatgata cgacgcagta cataaccacg
     2701 gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac gaatgtggtc
     2761 agcgattacg cgcagcgatt tattgctcag atcggtcgcg ccagtgactt tcgctaccgc
     2821 ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt gcagcaccgc
     2881 agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg gttccatcgt
     2941 gccatctgcc tggcggttga actgcatgaa gacgatgttc cagatctcaa tgtagcggtc
     3001 gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt gatcgtagaa
     3061 gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt tgtcagatgc
     3121 gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga tcccgacttc
     3181 tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc acagacgctc
     3241 tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact gaatggcatc
     3301 gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg tatggtgacg
     3361 cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca cgcagcgttg
     3421 ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat ccttgaactg
     3481 gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca gggagctgct
     3541 ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac ggatctcagc
     3601 ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca ccagctccag
     3661 cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt ttcttgactg
     3721 ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc tggatatctt
     3781 ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct gaaaagacag
     3841 ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg caccagtcga
     3901 tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc tgattcagtt
     3961 cctgacgaat acgtgcaggt ccataccctt tgcggctacg gctggcgata aagcgcgcaa
     4021 caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg cgctcgtaat
     4081 cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt gccgcgagtt
     4141 tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc gcacgatcca
     4201 acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat ccttacaact
     4261 taaaaaagca aaagggccgc agatgcgccc caggttttgc tgataaacag atttaatagt
     4321 caatgagtat gcagaatgtt cccgcaggcc caattcgtta gaggatcgag gtgagagtaa
     4381 tttttcacgc cagtgctggc ctgttcccct caccctaacc ctctccccaa aggggcgagg
     4441 ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa aatcttcgtt
     4501 agtttctgct actccttcgc tgtcatctac agagaaatcc ggcgttgagt tcgggttgct
     4561 cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt tatctttcag
     4621 ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc tgtaccacgc
     4681 gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc cgtagaagtt
     4741 gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag cgattttgtt
     4801 cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt tcaccgcgcc
     4861 gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac caccggtagt
     4921 ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga tgaagatcag
     4981 cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc tcatcatacg
     5041 tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt tcggcgtcag
     5101 tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca gggcgtcaca
     5161 gatttccagt gcctgctcgc cggtatccgg ctgggagcac agcaggttat cgatatcgac
     5221 gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa acgcacaggt
     5281 tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt taccggaaga
     5341 ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc caagcgcgat
     5401 atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt cttcacccag
     5461 gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg ccgccaacgc
     5521 tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta ataccggata
     5581 gtcaatatgt tctgttgaaa caattatact gtatgctcat acagtatcaa gtgttttgta
     5641 gaaattgttg ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc accgcatcac
     5701 ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg gcaaaagcaa
     5761 accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg ataccactaa
     5821 tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt tccaccacga
     5881 cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg atcatctgcg
     5941 ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag ctaccggcaa
     6001 tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt gttacggttg
     6061 cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt tcactgtcag
     6121 tcatcacaac ctctgtcagt caaaaattta tgccggacaa gatagcactt tcgtcaggaa
     6181 gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat tagccagagg
     6241 gaagctcacg cccccctctt gtaaatagct actgtactcg cgccagcgcc acggcttgtc
     6301 ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt gtaacgggtg atggtgtaga
     6361 agttcggcag accgtaccag tactggtagc cggtgccaac atccagacgc agcaggctgg
     6421 cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgcggcggca agctgcgaaa
     6481 tgctgtactt agttttgaaa ccatttggca agcctggagc ctgaccgttt gccattatcg
     6541 cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccacg ctaccgatcg
     6601 catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc gcatattgtt
     6661 tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa cctttcagat
     6721 tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc tcgccagaga
     6781 aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg aggatgcgag
     6841 ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg ataatttccg
     6901 gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga ttccagaaaa
     6961 ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc agccatgcgc
     7021 cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc atcagccgca
     7081 gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga cgatcgaaac
     7141 cgtgtttgtt caccattttg tcgatgaact gctgggcatt cgggttatta gcgaaatcgc
     7201 cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac ggcgttccgg
     7261 tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc aacacaaaaa
     7321 ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac agattcaacc
     7381 aggggcaagt atggtaaagc atcacacccc gcacaaggaa gcggtagtca ctgcccgata
     7441 cggactttac ataactcaac tcattcccct cgctatactt ttattcaaac tttcaaatta
     7501 aaatatttat ctttcatttt acgatc
//
LOCUS       DAZPEU010000180         7503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPEU010000180 DAZPEU010000000
VERSION     DAZPEU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.181"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            85..1137
                     /locus_tag="ACHU2L_003397"
     CDS             85..1137
                     /locus_tag="ACHU2L_003397"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR1358117.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1213..2646
                     /locus_tag="ACHU2L_003398"
     CDS             1213..2646
                     /locus_tag="ACHU2L_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR1358118.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2829..5009
                     /locus_tag="ACHU2L_003399"
     CDS             2829..5009
                     /locus_tag="ACHU2L_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR1358119.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(5150..6433)
                     /locus_tag="ACHU2L_003400"
     CDS             complement(5150..6433)
                     /locus_tag="ACHU2L_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR1358120.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(6568..>7503)
                     /locus_tag="ACHU2L_003401"
     CDS             complement(6568..>7503)
                     /locus_tag="ACHU2L_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1358121.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1793 a         1775 c         1917 g         2018 t
ORIGIN      
        1 cgttgagcta aagtcattag agatgctttg tccttaatgt aaccatatcg caataagtta
       61 tgtttttaaa ttgagggcat tattatgaaa aaaataccct gcgtgatgat gcgaggtgga
      121 acctcgaggg gcgcgttcct gttagcggaa catttacccg aagatcaaac gcagcgcgat
      181 aaaatattga tggcaattat gggttccggt aacgatctgg aaattgacgg tattggcggc
      241 ggtaatccac tgaccagtaa agtcgccatt attagccgtt ccagcgatcc gcgtgctgat
      301 gtcgattatc tgtttgctca agttatcgtc catgagcaac gtgtcgatac cacgcctaac
      361 tgcggcaata tgctgtctgg cgtaggggca ttcgccattg aaaatggttt gattgcagcg
      421 acttcgccag ttacccgtgt acgtatccgc aacgtcaata cgggtacgtt catcgaagct
      481 gatgtgcaaa cgccaaatgg tgttgtcgag tacgagggta gcgccagaat tgacggcgta
      541 ccgggtactg ccgcaccggt tgcgctcact ttcctgaatg ccgctggaac gaaaactgga
      601 aaagttttcc cgactgataa tcagattgat tattttgacg atgtcccggt gacctgtatc
      661 gatatggcga tgccagtcgt cattattccg gctgaatatc tgggaaaaac aggttatgaa
      721 ttaccggcgg aactggatgc cgacaaagca ttattagccc gcattgaatc tatccggcta
      781 caagcgggta aagcaatggg cttaggtgat gtcagtaata tggttatccc taaacctgtg
      841 cttatttctc cagcgcagaa aggcggggca attaatgtgc gttattttat gccgcattct
      901 tgccatcgcg cgctggcgat aaccggtgct attgctattt ccagtagttg tgcattggaa
      961 ggcaccgtca cccgacaaat cgtcccttct gtaggatatg gcaatatcaa tattgaacac
     1021 cccagtggtg cgctcgacgt tcatttaagt aatgaaggtc aggatgccac gacgttacgc
     1081 gcatctgtta ttcggacaac cagaaaaata ttttccggtg aagtttatct tccctgaaaa
     1141 aattcgttgt caggataagg acaatcaata aaggacttct gtatgagtca tacagaaaga
     1201 acaggatttt aaatgaataa gaaatcgtta tggaagctaa ttctgatatt agcgatccca
     1261 tgtattattg gttttatgcc agctccggca ggattaagcg aactggcgtg ggtgcttttt
     1321 ggtatttacc tggcggccat tgtgggtctg gttatcaagc ctttcccgga acctgtcgta
     1381 ctgttaattg ccgttgctgc ctcaatggtg gtggtcggta acttatccga cggtgcgttt
     1441 aagaccaccg ccgtattaag cggttactct tcaggtacta cctggctggt gttctcggcg
     1501 tttaccttaa gcgccgcatt tgtgaccacc ggtttaggta aacttattgc ctatctgctg
     1561 attggtaaaa tcggtaacac catgctgggt ctgggttacg ttacggtatt cctcgatctg
     1621 gtactggctc cggcaacacc gtctaacacc gcgcgtgcgg gcggcattgt gttaccgatc
     1681 atcaacagcg tggcggtggc tttggggtcc gaaccggaaa aaagtccgcg tcgtgtcgga
     1741 cattacctga tgatgtccat ttacatggtc accaaaacca ccagctatat gttctttacc
     1801 gcaatggcgg ggaacattct ggcgctgaaa atgatcaacg acattctgca cctgcaaatt
     1861 agctggggtg gatgggcgct ggccgccgga ttgccgggca tcattatgct gctggtcacc
     1921 ccgctggtaa tttacaccat gtatccacca gaaattaaaa aggtggataa caaaaccatc
     1981 gctaaagcgg gccttgccga actgggaccg atgaaaatcc gcgaaaaaat gctgctcggt
     2041 gtctttgtgc tggcgctact gggctggatt ttcagtaagt ctctgggggt tgatgaatcc
     2101 actgtggcaa tcgttgttat ggcgaccatg ctgctgctgg gtatcgttac ctgggaagac
     2161 gtggttaaaa ataaaggcgg ctggaatacc ttaatctggt acggcggtat tatcggctta
     2221 agctccttat tatcgaaagt taaatttttc gaatggttag ctgaagtctt taaaaataac
     2281 ctggcatttg atggtcacgg taacgttgct ttcttcgtta ttattttcct cagcatcatc
     2341 gtgcgttatt tcttcgcttc cggtagtgcc tatatcgttg ctatgttacc tgtatttgcc
     2401 atgctggcga acgtctccgg cgcaccgtta atgttaaccg cgctggcact gttgttctcc
     2461 aactcctatg gcggcatggt tactcactat ggcggcgcgg caggtccggt catctttggc
     2521 gtgggttaca acgatattaa atcctggtgg ttggtcggtg cggtactgac gatattaacc
     2581 ttcttggtgc atatcaccct cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg
     2641 ctgtaaatat actcgtcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
     2701 gtcacttact tatgtaagct cctggggatt cactcacttg tcgccttcct gcaactcgaa
     2761 ttatttagag tatatccatt tattatcttt ctgcgcactt cacggtgcgc agatatctgg
     2821 agcatttgat gatcaagtta tctgaaaaag gcgtgtttct cgccagtaat aacgaaataa
     2881 ttgccgaaga acatttcacc ggcgaaatta aaaaagaaga agcaaaaaaa ggcactattg
     2941 cctggtctat tctctcttct cataatacgt ccggaaatat ggataaactt aaaattaagt
     3001 ttgattcatt agcctctcac gatattacct ttgttggtat tgtacagacc gctaaagcgt
     3061 ccggaatgga acgtttcccg ctgccgtatg tgctgaccaa ctgccataac tcactctgcg
     3121 ccgtcggcgg caccattaac ggtgatgacc atgtttttgg tttatcggca gctcagcgtt
     3181 atggcggtat ttttgtgcct ccgcatattg cggtcatcca tcaatatatg cgtgagatga
     3241 tggctggcgg gggcaaaatg atcctcgggt cagacagcca cacccgttac ggtgcattag
     3301 ggacaatggc agtcggtgag ggtggcggtg agttggtaaa acagctgctt aatgacacct
     3361 gggatatcga ctatccgggc gtggttgcgg tgcatctgac cggaaaacct gcgccgtatg
     3421 tggagccaca ggatgtggcg ctggctatca ttggcgcggt gttcaaaaac ggttacgtca
     3481 aaaacaaagt catggagttc gttggaccgg gcgttagcgc gctctctacc gatttccgta
     3541 acagcgttga cgtgatgacc acggaaacca cctgtttaag ctccgtctgg caaactgatg
     3601 aagaagtgca taactggctg gcgctgcacg gtcgcggtca ggattactgc cagcttaacc
     3661 ctcaaccgat ggcgtactac gatggctgca tcagcgttga tttaagcgcc atcaaaccaa
     3721 tgattgcgct gccgttccac ccgagcaacg tgtatgaaat cgacacactg aaccagaacc
     3781 tgaccgacat tctgcgtgag attgaaattg agtccgaacg cgtggcgcac ggtaaagcca
     3841 aactctcgct gctggataaa gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg
     3901 cgggctgttc tggcggtaac tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc
     3961 aatcctgtgg caatgacacc ttctcgctgg cggtttaccc gtcatcacag ccggtgttta
     4021 tggatctcgc caaaaaaggt gtggtagcag atttgattgg cgcaggcgca atcatcagaa
     4081 ccgcattctg tggcccatgc tttggcgcgg gcgatacgcc aatcaacaac ggtttgagta
     4141 ttcgccacac cacgcgtaac ttcccgaacc gcgaaggctc taagccagcc aatgggcaga
     4201 tgtcagcggt ggcgttgatg gacgctcgtt ctatcgctgc gactgcggca aacggtggct
     4261 atttaacctc tgccagcgaa cttgattgct gggacaacgt gccggagtac gccttcgatg
     4321 taacgccgta taaaaaccgt gtttatcagg gctttgtgaa aggggcaact cagcaaccgc
     4381 tgatttacgg accgaacatt aaagactggc cggaattggg tgcgctgact gacaatatcg
     4441 tcctgaaagt gtgctcgaag atcctcgacg aagtcaccac caccgacgaa ctgattcctt
     4501 ccggtgaaac ctcttcttat cgttcaaatc cgattggtct ggcggggaat gtcagcgagc
     4561 tgacagaggt gtttgcgcgc attaagcaga ttgctggtca ggagcatatt gatccgctgc
     4621 aaactgaaat tggtagcatg gtctatgcgg tgaaaccagg cgatggttct gcgcgtgaac
     4681 aggcggcgag ctgccagcgt gtgattggcg gtctggcgaa tattgccgag gagtacgcga
     4741 ctaaacgcta ccgttctaac gtcatcaact gggggatgtt accgctgcag atggcggaag
     4801 tgccaacctt tgaagtgggg gattacattt acatccctgg cattaaagcg gcgctggata
     4861 atccgggtac gacgtttaaa ggttatgtga tccatgaaga tgcgccggta acggaaatta
     4921 cgctttacat ggaaagcctg acggctgaag agcgcgagat tatcaaggcg ggtagtttga
     4981 ttaacttcaa taaaaaccgt cagatgtaaa aagcgccatg tgaatgtagg tcgcattcgg
     5041 cacttattgt cggatgcgat gctggcgcat cttatccgac ctacaaatcg catcgaaccg
     5101 taggccggat aaggcgttta cgccgcatcc ggcaaatagt taatagctct tacttcttct
     5161 cctctgcaac cactttgctg cccacgccgc ggttattgta ttcccacatg cggttgtagt
     5221 tagtgtcatt cagattgcgc tgtatttcgt cgttatcatc tacgctgccg gtattacccg
     5281 caaacggacg attagagatc accgcatcgg cccacggttt agccgtgtta aaaccttcgt
     5341 tgatggcgct atcacggatc accacctgac cgttggtatt agcatcaaca tccagcgagc
     5401 ggcccagttg cgccacgcca tcaccggaag cattgaaacg gctgtttacg gcgaggaaac
     5461 cgtaatagat gttagacagc gtagccggtg caaacacata tgcttcttgc tgggtacgtg
     5521 agttcaccac gcggaattcg gtgttatcga acaccactgc gccgcgacca gaaacgatat
     5581 ccacatcccc ttcaatatag ctgttggtca ccagcgtacg cggctgacgg ttggtttcca
     5641 gacggttctg cacaccgctg ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa
     5701 cgttgttaat ctgtacttgg tcaccgtcag tacgcagtgc taccgccgga tggttacctg
     5761 catctacgct atcgcccagc gtgttttcga tggtcagatt ttgcagttgc agtccattgt
     5821 tttgtgacca gaagaccgca gaacacagaa caccgatact gtcgctgcgt ttgctctggc
     5881 agctatcgta catataccac gctggtttac ctggcatata tttgccgcgc gggttgacgt
     5941 cgtgacgcca gtcggcaggg ctcatgcccc catcaaggga aagcccaatc ttcacatcaa
     6001 tcggtttttc acctgtaccg tacagagtga ttccacctgg agcagcaggg acatacaccg
     6061 ttccctgata ctcaccaggc atcacggcaa tatactggcg cttgttggtg cgcttgataa
     6121 ttgccgcatc taccgccgcc tgaatcgtgg tatgcgttac accttgagta cccgccgggc
     6181 cgacaacaaa gtcaggttgc gcaggcaggg ttatcgggga cggattccac gctgcagcac
     6241 ctggtgtcag ggatgcaaaa tagtgttgcg catcgaaatt cagcgcttct tttgccgaca
     6301 gaatcgggcg agaagaggta ccaggcgcag tttgatcaga aggacgttga tcgggtggtg
     6361 ttgagctaca ggcggtcagc gtcacgccaa aagccaatgc cagcgccaga cgggaaactg
     6421 aaaatgtgtt cacaggttgc tccgggctat gaaatagaaa aatgaatccg ttgaagcctg
     6481 cttttttata ctaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac
     6541 aggtcattat cagtcaaaat aaaatcatta tttgatttca attttgtccc actccctgcc
     6601 tctgtcatca cgatactgtg atgccatggt gtccgacttg tgcccgagaa gatgttgagc
     6661 aaacttatcg cttatctgct tctcatagag tcttgcagac aaactgcgca actcgtgaaa
     6721 ggtaggtgga tccccttcga aggaaagacc tgatgctttt cgtgcgcgca taaaatacct
     6781 tgatactgtg ccggatgaaa gcggttcacg acgagtagat gcaattatgg tttctccgcc
     6841 aagaatctct ttgcatttat caagtgtttc cttcattgat atcccgagag catcaacatg
     6901 caatgttgta gggatggcaa tttttacgcc tgttttgctt tgctcgacat aaagatatcc
     6961 atctacgata tcagaccact tcatttcgca taaatcacca actcgttgcc cggtaacaac
     7021 agccagttcc attgcaagtc tgagccaaca tggtgatgat tctgctgctt gataaatttt
     7081 caggtattcg tcagccgtaa gtcttgatct ccttacctct gattttgctg cgcgagtggc
     7141 agcgaccggg tttgttgtta tatggccttc agctattgcc tctcggaatg catcgctcag
     7201 tgttgatctg attaacttgg ctgatgccgc cttgccctcg tctatgtatc cattgagcat
     7261 tgccgcaatt tcttttgtgg tgatgtcttc aagtggagca tcaggcagcc ccctccttat
     7321 tgctttaatt ttgctcatgt aatttatgag tgtcttctgc ttgattcctc tgctggcgag
     7381 gattttttcg tagcgatcaa gccatgaatg taacgtaaca gaattatcac tgttgattct
     7441 cgctgtcaga ggcttgtgtt tgtgtcctga aaataactca atgttggcct gtatagcttc
     7501 agt
//
LOCUS       DAZPEU010000181         7424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPEU010000181 DAZPEU010000000
VERSION     DAZPEU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.182"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            143..463
                     /locus_tag="ACHU2L_003402"
     CDS             143..463
                     /locus_tag="ACHU2L_003402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358122.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(529..761)
                     /locus_tag="ACHU2L_003403"
                     /pseudo
     CDS             complement(529..761)
                     /locus_tag="ACHU2L_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(871..1641)
                     /gene="yafV"
                     /locus_tag="ACHU2L_003404"
     CDS             complement(871..1641)
                     /gene="yafV"
                     /locus_tag="ACHU2L_003404"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR1358123.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1822..2268
                     /gene="ivy"
                     /locus_tag="ACHU2L_003405"
     CDS             1822..2268
                     /gene="ivy"
                     /locus_tag="ACHU2L_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR1358124.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2311..4755)
                     /gene="fadE"
                     /locus_tag="ACHU2L_003406"
     CDS             complement(2311..4755)
                     /gene="fadE"
                     /locus_tag="ACHU2L_003406"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR1358125.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4995..5573
                     /gene="lpcA"
                     /locus_tag="ACHU2L_003407"
     CDS             4995..5573
                     /gene="lpcA"
                     /locus_tag="ACHU2L_003407"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR1358126.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5779..6546
                     /locus_tag="ACHU2L_003408"
     CDS             5779..6546
                     /locus_tag="ACHU2L_003408"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR1358127.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6517..7257)
                     /gene="dpaA"
                     /locus_tag="ACHU2L_003409"
     CDS             complement(6517..7257)
                     /gene="dpaA"
                     /locus_tag="ACHU2L_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR1358128.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
BASE COUNT         1834 a         1937 c         1868 g         1785 t
ORIGIN      
        1 ttggtttaga atcaagcaag aaaattagaa aagaacttgg taatattctt cagaatatgg
       61 ctaggaataa tgaggtgtta gttcatgggc aaattcttgg aaataaagtt agccgatgta
      121 attagttagt ggagaggaag ttgtggacga aattagaata agaattgctg gtaatacggc
      181 tgcgccttgt ttccatgtgc taaaaaataa gggatataaa gtaacaatgg aaactcatgc
      241 attatctgac gattgtttag attgtgtata tgattggtat gcggaaaaaa atggacaatt
      301 ttttagtgca acgagtccag aagagttact tggattagtt gcaatgtggg aagttcgtgg
      361 taatgactgg agagctaatt tgaatgagat aaatacttat caaaaagcca tagaagaatc
      421 cccgtttttt gataaaaacg gcaatgaaat agagtctgac taaattttat tgatatagca
      481 acgataataa ccggaaattt cttccagatc tttccggctt tttattattt aactttcgtt
      541 cagcacacta aacatcccgt ctcgcatccc cttaactggc tgtttaacct gataaagttc
      601 cctgcgcagt tcctgtactt cgttgttatc ccaagcagga tcaaacatcc ctccgagatc
      661 ttcacggtaa cgcccgtgcc gatctcaaat cccgcttttc tcagccattt ggcgctgatt
      721 ttgatcaccg gcgtgtcgct gcggttgggc agataaccca cggtacagcg acgctgtgtt
      781 ttggaaatgg tgacttctgg cgtaatattg tgtttaagtt aggggctttc gtaatctagc
      841 ccagcccccg gccctaaaca caacccccac tcaccacaac ctaaactcat ccgcatcccg
      901 ccatgccgga aacttttctc tatattcccg caatgccacc atcgacagct ccgcatcaat
      961 acgcgttgcc ttatgcgcgt cggcggtagc gataatctcg ccttgcggat taatcacccg
     1021 gctgtcaccg cgataatggc agccgttgcc atcgctgccg acgcgattgc atcccgccac
     1081 atacgcctga ttctcaatcg cgcgggccgt cagcaatgcc tgccagtgca gagagcgcgg
     1141 agctggccag ttggcgacgt acagggcgag gtcataatcg ttgagattgc gcgaccacac
     1201 tggaaaacgt aagtcgtagc acaccagcgg caaaatacgc cagccgcgcc attccacaat
     1261 cactcgcgca ttgcccgctt tataatgcag atgctcatct gccatgcgga acagatgacg
     1321 cttatcataa aaatgtaccg tgccgcccgg ctcaaccagc aaaaagcggt taaccgaacc
     1381 agactccgtt tgtaatgcaa cactgcctgc aatcagcgca ttgcactgct gcgccttagc
     1441 tgtcatccag ttcactacgt catcttgtgc aagcgacgaa gctgccgctt ccatggcaaa
     1501 gccgctggta aacatctccg gtagaacgat cacatcgcgc ccggtaatac cttccagttg
     1561 acgatcaaaa tggcgcaggt tggcaggacc atccacccac accaacggtt gctgcaaaag
     1621 cgtaatcttc aaactaggca cggtgtacaa ctcctttatg cgaagggttt tataacttta
     1681 acaccttatc aggcagttgc cttagcgcag aataaattga taacaaatgc tgatattgga
     1741 aatatctgat ttgcaaatta tcgtgttatc gctaggcttt aggaggttaa taacatgggc
     1801 aggataagct cgggaggaat gatgtttaag gcaataacga cagtcgccgc actggtcatc
     1861 gccaccagtg caatggcgca ggatgattta accattagca gccttgcaaa gggcgaaacc
     1921 accaaagctg cgtttaatca gatggtgcaa gggcataagc tacctgcatg ggtgatgaaa
     1981 ggcggtactt atactcccgc acaaaccgta acgttgggag atgagacgta tcaggtgatg
     2041 agcgcgtgca aaccgcatga ctgtggctcg caacgtatcg ctgtgatgtg gtccgagaaa
     2101 tctaatcaga tgacggggct gttctcggct attgatgaga aaacgtcgca agagaaactc
     2161 acctggctga atgtgaacga tgcgctttcg attgatggta aaacggtgct gttcgcggcg
     2221 ttgaccggca gcctggaaaa ccatccggat ggctttaatt ttaaataatt agcggataaa
     2281 gaaacggagc ctttcggctc cgttattcaa ttacgcggct tcaactttcc gcactttctc
     2341 cggcaacttt accggcttcg tcgccagctc ttccggatca aagtcatcaa cgttaatact
     2401 gcgcagacgg ctttcttccg ctttcaccag aatagcggct tcatctttat caatcagccc
     2461 cttcgtcagc gcgttgtgcg ccagttcatc cagacgggta aatggcagat ttttacccag
     2521 ctctttacag atccgctgat gaatcgggtc ggcggcaatc acatccacca acgcctcttc
     2581 cagtaaacca accgggttat gctcgcttgg cgtcaggtac tgaccgcgac caatacgtga
     2641 acgagtggcg ttcggcactt gtaaaatctt cgccatttta tgatccagct tgtcagacgg
     2701 tgccagataa tgacgtccgg tcgggaagat caccacattc agcagcccgg caaccacgcg
     2761 gttcgggaag ttttgcagta aattgtccat tgcctgttct gcctgataca gcgcatcctg
     2821 tacgccccag tgcaccagcg gcagatcggc ttcattacgg ccttcgtcgt cataacgctt
     2881 cagaacggca gaggcgaggt aaagctggct taaaatatcc cccagacgtg ccgagatacg
     2941 ctcacgacgt ttcaggctgc cgcccagcag tgccatcgag acatcagaaa gcagggcgag
     3001 gttggcgctc aggcggttca ggtgctgata gtagcgttta gtggcatcgc cggttggcgt
     3061 gctgctggtt aaaccgcgcg tcaggcccag ccagaagctg cgaactttgt tgctaccgac
     3121 gtgaccgata tgtttgaaca acagtttatc gaacgcgttg acgtcattgt tcttcgccgc
     3181 ttccatctct tccagcacgt acggatggca acgaatcgct ccttgtccga agatcatcat
     3241 gctgcgggtc agaatgttag ccccttcaac ggtgatggca atcggtgcgc cctggtaagc
     3301 acgcgccagg aagttgcttt gcccgagcat aatgccttta ccgccggtaa tatccatcgc
     3361 atcaataatc gactgctgcc cgcggtgggt acagtgatac ttaacgatag ccgacagcac
     3421 ggcaggtttt tcgccgagca taatgccgta ggtaatcagc gatgccgcag catccattac
     3481 ataagcgttc ccggcaatac gcgccagcgg ctcttcaatc ccttccatct taccgataga
     3541 gattttgaac tgacggcgaa tgtgagaata cgcgcccgtt gccagcgcta ccgatttcac
     3601 gccgccggtt gagttggaag gcagggtgat gccgcggcct accgagaggc actccaccag
     3661 catccgccag ccttgcccgg ccattttcgg cccgccgatg atgtaatcga tcggcacgaa
     3721 gacatcttta ccgcgcgtcg gtccgttctg gaacggtacg ttcagcggga agtggcgacg
     3781 accaatttcc acgcccggcg tggtggttgg gatcagcgca caggtaatgc ctaaatcttc
     3841 tgcaccgccg agtaattttt ccgggtcgga gagtttaaac gccagcccaa gcacggtcgc
     3901 aatcggtgcc agcgtaatgt agcgtttgtt ccaggtcaga cgcatcccca gcacctgctg
     3961 gccctgccat tcgcccatgc agacaatccc ggtgtccgga atcgcgcccg catcggaacc
     4021 cgcttccggg ctggtcagtg caaagcaggg gatctcctga ccacgcgcca gacgcggcag
     4081 atagtgatct ttctgctcgt cagtgccgta atgttgcaac agttcgcccg ggcctaatga
     4141 gtttggcacg ccgacggtaa tcgccaggat cccgctcacg ccagagagtt tttgcagcac
     4201 acgagactgg gcataagctg agaactccag tccgccgtac tcttttttga taatcatcgc
     4261 gaagaagcga tgctctttaa ggtacgccca caactccggt ggcagatccg ccagctcatg
     4321 agtaatctgg aaatcattcg ccatccggca ggcttcttct accgggccgt cgagaaacgc
     4381 ttgctcttca gcggtcaggc gcggctgcgg atagttatgc agctttttcc agtccggctt
     4441 gccctggaac aagtcgccct cccaccaggt ggtgcccgca tcaatcgctt ctttctcagt
     4501 gcgcgacatc ggcggcatca ccttacggaa accgcgaaat accggcgcgg aaatcatcga
     4561 cttacgcata ggcgcaaagt taaatggcac gaggataatg gtcagaggca ccagtaccca
     4621 cgccgaccac agaccagcaa cgccgagggc ggctgtccag gcgagcaaaa tcagactgct
     4681 gataaataag ctcacgcggt gatagaacaa cgcgccgagc aggacaaccg tagcgagaat
     4741 actcaaaatc atcataacga aaagcccctt acttgtagga ggtctgacca cttgtgatga
     4801 tatggttgta gtggatgtaa aaacatttag caatgtgttt acaatataat tacaacaaag
     4861 ctcacattgt tgctgttttt atccgcactt caggtcaaaa agtcctggtc atagcacctg
     4921 cccgtacttc tcgcttttgg cggtatccgg tacactgcat tttgtctatt acatttatgc
     4981 tgaaggatat cctcatgtac caggatctta ttcgtaacga actgaacgaa gcggcggaaa
     5041 cgctggctaa ctttttaaaa gatgacgcca atattcacgc cattcagcgc gcggcggtcc
     5101 tgttagcaga cagctttaaa gccggtggca aagtgctttc ctgcggcaac ggcggttccc
     5161 attgcgacgc tatgcacttt gccgaagagt tgaccggtcg ctaccgtgaa aaccgtccgg
     5221 gctacccggc gattgctatt tctgacgtta gtcatatttc ctgcgtcggt aatgatttcg
     5281 gtttcaatga tattttctcc cgctacgttg aagcggtagg tcgcgaaggc gatgtactgc
     5341 tggggatctc cacctccggt aactctgcaa acgtgatcaa agcgatcgca gcggcgcgtg
     5401 agaaggggat gaaagtgatc accctgaccg gtaaagacgg cggtaaaatg gctggcacgg
     5461 cggatatcga aattcgcgtt ccgcactttg gttatgccga ccgcattcag gagattcaca
     5521 ttaaagtgat ccatatcctg atccagttaa ttgaaaaaga gatggttaag taagtctggc
     5581 gtaggccgga taaggcgttt acgccgcatc cggcacttgt gcgctgatgc ctgatgcgat
     5641 gctgacgcgt cttatcatgc ctacaaatct gtacccgaac cgtaggccga ataattcgtt
     5701 tacgccgcat ccgacctgaa aattcttaaa tcaatcttcg ccggggtcca tgcgctcccg
     5761 ctgttgtgga ggttacccat gtgcgaattg ctcgggatga gcgccaacgt ccctaccgat
     5821 atctgcttta gtttcaccgg acttgtacag cgtggtggtg gaaccgggcc acataaagat
     5881 ggctggggca ttacctttta cgaaggtaaa ggctgtcgca catttaaaga tccacaaccc
     5941 agctttaatt cccccatcgc caaacttgtc caggactacc cgataaaatc ctgttcggtg
     6001 gtggctcata ttcgccaggc taatcggggc gaggtggcgc tggaaaatac tcacccattt
     6061 acccgcgagt tatgggggcg taactggact tatgcccata acggacaact gacgggctac
     6121 aaatcactgg aaaccggcaa cttccgcccg gtcggtgaaa ccgacagcga aaaagccttt
     6181 tgctggctgc tgcataaatt aacgcagcgt tacccgcgca cgccgggcaa catggcggca
     6241 gtgtttaaat atatcgcctc actggcggat gaactgcggg cgaagggcgt tttcaacatg
     6301 ctgctttcgg acgggcgcta tgtaatggcg tattgctcga ctaatttaca ctggatcacc
     6361 cgccgcgcgc cgtttggcgt ggcaacgttg ctggatcagg atgtggaaat cgacttcagc
     6421 tcgcagacca caccgaatga tgtggtcacg gtgattgcga cacagccgct gacgggcaat
     6481 gaaacctggc aaaaaattat gccaggcgaa tggcgcttat tttgcctcgg ggagcgtgta
     6541 gtttgatgcc agttgtggct gcaccacttc gtggcttaac ggcttgctga ccacgtaacg
     6601 gccattggcc acagaaacgg tcggtggctt acgggtttgc tcaaagtagt cgtagcccgg
     6661 cttcagctgc tcccagaagt ccttaaagtt ggaatattta tggcgcttca tattggcgtc
     6721 ggtcatgcgg aatgggtaaa tactcacctg cacgctcggc tgaccaaaca ccagcgcacc
     6781 agtaacgaac tggaagatct catcaatacc ctgattggtc attgcgtagc agccgatgga
     6841 tacacaatcg ccgtggatca tcaggtattt cccttcataa ccatgcgcac ggtcataagc
     6901 attggggaat ccaatattaa tcgctttgta gtaacggctg tctggtttta actgattacg
     6961 ctggacgcta taaaaccctt ccgggctttt gaaatcgccc tgacgctgtt ttggccctaa
     7021 gccgccggaa tatttacaga ttttataact gtcgagcagt tgatattgct cgcccatttt
     7081 gacgtaaaga tcgagcgtac gttcttcctt gaagatctgg atgtagacag gggatcccat
     7141 caactgctgc ttatactctt tgctcactgg cgtggtcgag ctactgctgc ccagcaaacc
     7201 ggcaaacgaa acgcacggga tcaacaacat cgcaagaatt aatgcgattt tacgcatact
     7261 gcttattcct tgataaaacg gttacacacg ccaggacggc aaaatggatc ccaaatcgga
     7321 atagtctgga tttggaaggc tcacattatc accaaaagag ttttacgcaa gcctgtcggc
     7381 gctgggttta caaatttagc acaggggcag ttttcaatag tccg
//
LOCUS       DAZPEU010000182         7390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPEU010000182 DAZPEU010000000
VERSION     DAZPEU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.183"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(78..557)
                     /gene="folK"
                     /locus_tag="ACHU2L_003410"
     CDS             complement(78..557)
                     /gene="folK"
                     /locus_tag="ACHU2L_003410"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR1358129.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(554..1951)
                     /gene="pcnB"
                     /locus_tag="ACHU2L_003411"
     CDS             complement(554..1951)
                     /gene="pcnB"
                     /locus_tag="ACHU2L_003411"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR1358130.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(2011..2907)
                     /gene="gluQRS"
                     /locus_tag="ACHU2L_003412"
     CDS             complement(2011..2907)
                     /gene="gluQRS"
                     /locus_tag="ACHU2L_003412"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR1358131.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(2974..3429)
                     /gene="dksA"
                     /locus_tag="ACHU2L_003413"
     CDS             complement(2974..3429)
                     /gene="dksA"
                     /locus_tag="ACHU2L_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR1358132.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3607..4311)
                     /gene="sfsA"
                     /locus_tag="ACHU2L_003414"
     CDS             complement(3607..4311)
                     /gene="sfsA"
                     /locus_tag="ACHU2L_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR1358133.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4326..4856)
                     /gene="thpR"
                     /locus_tag="ACHU2L_003415"
     CDS             complement(4326..4856)
                     /gene="thpR"
                     /locus_tag="ACHU2L_003415"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR1358134.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4930..7359
                     /gene="hrpB"
                     /locus_tag="ACHU2L_003416"
     CDS             4930..7359
                     /gene="hrpB"
                     /locus_tag="ACHU2L_003416"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR1358135.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1688 a         1909 c         2071 g         1722 t
ORIGIN      
        1 tttaaactaa aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata
       61 ttcgtaatat aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact
      121 tcacgcaacg tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac
      181 agcataaatc cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc
      241 acttcattac caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct
      301 ttacgaacac gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg
      361 gcgggcgaag tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc
      421 agcggtgggg tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct
      481 aatgctttca gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca
      541 atatacgcca ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag
      601 tacgacgacg cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg
      661 gtgcggaaac ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt
      721 caacttcagc tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca
      781 gtttccatgc gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc
      841 gggttaacgt cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt
      901 cgttcatcgc cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct
      961 gtgccgtctc cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc
     1021 gcatatcgtt atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca
     1081 tcgggctgtc gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca
     1141 gatgatattc acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa
     1201 gcgattcttc aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga
     1261 tcggttctgc ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc
     1321 gcagcatacg taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa
     1381 cgccgtcctt cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc
     1441 tgtaatacag gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga
     1501 aaatgttgtc gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga
     1561 cgttaccttc gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta
     1621 catgagccag acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct
     1681 gctcaggcgt ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt
     1741 cgcgcacgcc gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta
     1801 ccttcagggc attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca
     1861 ccgtcacctg tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct
     1921 tgcggcaaaa attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt
     1981 caggacaaaa aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt
     2041 tacaattgcc gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat
     2101 ttgctcgacg ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc
     2161 cgcgattagt accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga
     2221 aagtttagcg ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca
     2281 gccaaaaagc tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg
     2341 cactatttct gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa
     2401 caacccatca cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat
     2461 aatgcctcgc agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc
     2521 ggcgttgtct ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct
     2581 ttgaatacgc gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc
     2641 gagtgcttca cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg
     2701 cagaccgtaa tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg
     2761 cgggtcgata tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata
     2821 gctgccgagc gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa
     2881 gcggccaata tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc
     2941 ccgcctgtgg taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat
     3001 ttcagccagc gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg
     3061 aataccaatt tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt
     3121 cagcgtcttc tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag
     3181 gctgaactct tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc
     3241 ctgcatatgt gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag
     3301 aatacgacgg aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg
     3361 atatggttcc accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc
     3421 ttcttgcatg ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg
     3481 aggccgctat aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac
     3541 aactgtgtga ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt
     3601 actttactac aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc
     3661 cgctttgtaa gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta
     3721 tttctcatcg atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa
     3781 aaaaataacc gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg
     3841 acctcgttca gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga
     3901 tttcacttca atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct
     3961 gcgttcggag ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat
     4021 tgattcatta aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat
     4081 aaatgcgccg ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt
     4141 gtctgaagtc gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt
     4201 attcgggcag tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg
     4261 tttgtaacgc tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat
     4321 ccttgttatt gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca
     4381 aacgaggagg cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc
     4441 gggaatgtca ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga
     4501 ttgctttgaa aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt
     4561 aagccgcgtg gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg
     4621 gcgtcatcaa gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc
     4681 gccttctctt tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg
     4741 gcggcgaccg gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt
     4801 tcgcggattt ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga
     4861 ggcactcggt tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg
     4921 gagcgttttg tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc
     4981 gattgtgcgc cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg
     5041 ccgctgcaac tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt
     5101 cgtctggcgg cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc
     5161 gataccgttg gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa
     5221 gtggttaccg aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt
     5281 ggattggtga tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg
     5341 ctgttactcg atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct
     5401 acgctggaca atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa
     5461 gggcgctcgt ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac
     5521 gaagccgttg cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta
     5581 tttttacctg gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc
     5641 agtgatgtgg tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg
     5701 atcctcccgg caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc
     5761 agtttaacca tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc
     5821 tttgatccgc gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg
     5881 acgcagcgtg ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc
     5941 aaagaacaag cagaacgcgc cgcggcgcaa agtgagccga agatcttaca aagcgatctt
     6001 tccggtttgc tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg
     6061 ctggatcaac cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg
     6121 gcgctggagg gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat
     6181 ccgcgtttag cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca
     6241 aaaattgccg ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg
     6301 ttttcgcgca atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac
     6361 gtacgcggcg gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc
     6421 gatcgcattg ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga
     6481 gcgatgctcg atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta
     6541 ttgcagggca gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat
     6601 gaattagtac aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac
     6661 gcgcaaggta cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg
     6721 cagccgctgg cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt
     6781 gataaaggtt taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg
     6841 ttatgcgccg caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg
     6901 ttggcgacgc tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc
     6961 ctgaaatcac tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa
     7021 cgtctggata gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt
     7081 cgttatcatg aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag
     7141 gccactaatc cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct
     7201 gcccaaaggc cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt
     7261 gaggtgcaaa aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca
     7321 aacacggcac cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc
     7381 tgtgggagag
//
LOCUS       DAZPEU010000183         7375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPEU010000183 DAZPEU010000000
VERSION     DAZPEU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.184"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1541
                     /locus_tag="ACHU2L_003417"
     CDS             <1..1541
                     /locus_tag="ACHU2L_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1358136.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACHU2L_003418"
     CDS             1593..2750
                     /locus_tag="ACHU2L_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1358137.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACHU2L_003419"
     CDS             complement(2716..2838)
                     /locus_tag="ACHU2L_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358138.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="ACHU2L_003420"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="ACHU2L_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="ACHU2L_003421"
     CDS             4764..7124
                     /locus_tag="ACHU2L_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1358139.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..>7375
                     /locus_tag="ACHU2L_003422"
     CDS             7181..>7375
                     /locus_tag="ACHU2L_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358140.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2163 a         1615 c         1568 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattg
//
LOCUS       DAZPEU010000184         7373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPEU010000184 DAZPEU010000000
VERSION     DAZPEU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.185"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            118..1515
                     /gene="ulaA"
                     /locus_tag="ACHU2L_003423"
     CDS             118..1515
                     /gene="ulaA"
                     /locus_tag="ACHU2L_003423"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR1358141.1"
                     /translation="MEILYNIFTVFFNQVMTNALLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1531..1836
                     /gene="ulaB"
                     /locus_tag="ACHU2L_003424"
     CDS             1531..1836
                     /gene="ulaB"
                     /locus_tag="ACHU2L_003424"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR1358142.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1846..2310
                     /gene="ulaC"
                     /locus_tag="ACHU2L_003425"
     CDS             1846..2310
                     /gene="ulaC"
                     /locus_tag="ACHU2L_003425"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR1358143.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2324..2974
                     /gene="ulaD"
                     /locus_tag="ACHU2L_003426"
     CDS             2324..2974
                     /gene="ulaD"
                     /locus_tag="ACHU2L_003426"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR1358144.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2984..3838
                     /gene="ulaE"
                     /locus_tag="ACHU2L_003427"
     CDS             2984..3838
                     /gene="ulaE"
                     /locus_tag="ACHU2L_003427"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR1358145.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            3838..4524
                     /gene="ulaF"
                     /locus_tag="ACHU2L_003428"
     CDS             3838..4524
                     /gene="ulaF"
                     /locus_tag="ACHU2L_003428"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR1358146.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4653..4928)
                     /gene="yjfY"
                     /locus_tag="ACHU2L_003429"
     CDS             complement(4653..4928)
                     /gene="yjfY"
                     /locus_tag="ACHU2L_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR1358147.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5254..5649
                     /gene="rpsF"
                     /locus_tag="ACHU2L_003430"
     CDS             5254..5649
                     /gene="rpsF"
                     /locus_tag="ACHU2L_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR1358148.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5656..5970
                     /gene="priB"
                     /locus_tag="ACHU2L_003431"
     CDS             5656..5970
                     /gene="priB"
                     /locus_tag="ACHU2L_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR1358149.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5975..6202
                     /gene="rpsR"
                     /locus_tag="ACHU2L_003432"
     CDS             5975..6202
                     /gene="rpsR"
                     /locus_tag="ACHU2L_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR1358150.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6244..6693
                     /gene="rplI"
                     /locus_tag="ACHU2L_003433"
     CDS             6244..6693
                     /gene="rplI"
                     /locus_tag="ACHU2L_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR1358151.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6764..7327)
                     /locus_tag="ACHU2L_003434"
                     /pseudo
     CDS             complement(6764..7327)
                     /locus_tag="ACHU2L_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         1756 a         1799 c         2016 g         1802 t
ORIGIN      
        1 ggtcgcgtca catttaatca taaataatct tgttgtgatt acttttgaaa attagagtga
       61 gtgcacaaca ttccgggtgt gcggaatacc cggttacctc ttcttcagga gatcgttatg
      121 gagatcctct acaacatctt taccgtgttt tttaaccagg tcatgaccaa tgccctgttg
      181 ttgctgggta ttgtgacctg tctgggctac atcctactgc gcaaaagtgt cagcgttatt
      241 attaaaggca cgattaaaac cataattggt ttcatgttgt tgcaggcagg gtccggcatc
      301 ctcaccagca ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg cattaacggc
      361 gcaatttccg atacttacgc gtcgatgatg gcaaccatcg accgcatggg cgatgcctat
      421 agctgggtag gttacgcggt gttgttagcg ctggcgctga acatctgtta cgtgctgctg
      481 cgtcgcatta ccggtattcg taccatcatg ctgactggtc atatcatgtt ccagcaggcg
      541 gggctgattg ccgttacgtt gtttattttc ggctactcca tgtggacaac cattatctgc
      601 acggcgattc tggtttcgct ctactggggc attacctcca acatgatgta caagccgact
      661 caggaagtga cggacggctg cggtttctcc atcggtcacc agcaacagtt tgcatcatta
      721 attgcctata aagttgcgcc gttcctcggc aaaaaagagg aaagcgttga agacctcaaa
      781 ctgccaggct ggctgaatat tttccacgac aacatcgtct ccacggcgat tgtcatgacc
      841 atcttctttg gtgccattct gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc
      901 aaagtgcact ggacggtcta tatcctgcaa accggttttt ccttcgcggt ggcgatcttc
      961 atcatcactc agggtgtgcg catgtttgtg gcggaactct ctgaagcgtt taacggtatc
     1021 tcccagcgcc tgatccctgg tgcggttctg gcgattgact gtgcggctat ttatagcttc
     1081 gctccgaacg ccgtggtctg gggctttatg tggggcacca tcggtcagct gattgcggtt
     1141 ggcatcctgg tcgcctgcgg ttcctcgatc ctgattatcc caggctttat cccgatgttc
     1201 ttctccaacg ccaccatcgg cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg
     1261 aagatttgtc tggtgatggg gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt
     1321 accggtatga gtgcctggat ggggatggcg gactggtcga ttctggcacc gccgatgatg
     1381 cagggcttct tctccatcgg tatcgccttt atggccgtca tcattgtaat tgcactggct
     1441 tatatgttct tcgctggccg cgcactgcgc gcagaagaag atgcagaaaa acaattggca
     1501 gaacagtctg cttaataagg agttttgatt atgaccgtac gtattctggc tgtgtgtggc
     1561 aacggacaag gcagttccat gatcatgaag atgaaagtgg accagttttt aacccaatca
     1621 aacattgacc acacggtaaa tagctgcgcg gttggcgaat acaaaagcga gttgagcggc
     1681 gcggatatca tcatcgcttc tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa
     1741 tacgtggttg gcgtgcgcaa catgctctct cctgccgact ttggcccgaa actgctggaa
     1801 gtgatcaaag cgcatttccc gcaggatgtg aagtaaggac gcgccatgaa attacatgat
     1861 tcgctggcgg agaataaatc catccgcctg caggctgaag ctgagacatg gcaggacgca
     1921 gtaaaaatcg gtgttgacct gctggtggcg gcggatgttg tcgagccgcg ttactaccag
     1981 gcgattctgg atgcggttga acagcacggt ccgtactttg tgctcgcacc aggcctggca
     2041 atgccgcacg gtcgcccgga agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg
     2101 aaaaagccgc tggagttcaa ccacgaagat aacgacccgg tggatatcct catcaccatg
     2161 gcggcggtcg atgccaacac gcaccaggaa gtgggcatca tgcagatcgt caacctgttt
     2221 gaagatgaag aaaatttcga ccgtttacgt gcctgccgta ccgagcagga agtactggat
     2281 ctcattgacc gcaccaacgc ggcagcttaa gaaggaattg aagatgtcat taccgatgtt
     2341 gcaagtcgcg ctggacaacc agactatgga tagcgcctac gaaaccactc gcctgattgc
     2401 cgaagaagtc gacattatcg aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc
     2461 ggttcgcgat ctgaaagcgc tctacccgca caaaatcgtg ctggcagacg ccaaaattgc
     2521 cgatgcgggc aaaattcttt ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt
     2581 gatttgttgt gcggatatca acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa
     2641 cggcgacgtg cagatcgaac tgaccggtta ctggacctgg gaacaggcgc aacagtggcg
     2701 cgacgcaggt attcagcagg tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt
     2761 ggcgtggggt gaagccgata tcactgcgat caaacgtctt tccgatatgg gcttcaaagt
     2821 caccgtcacc ggtggcctgg cgctggaaga tctgccgctg ttcaaaggca ttccaattca
     2881 tgtgtttatc gccggtcgca gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca
     2941 gttcaaacgt tccatcgccg aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat
     3001 cccgcttggc atctatgaaa aagcgctccc cgccggggag tgctggctgg aacgcctgcg
     3061 actggcaaaa acgttgggct tcgattttgt cgagatgtcg gtagatgaaa ctaacgagcg
     3121 cctgtcgcgc cttgactgga gccgcgaaca gcgtctggcg ctggtgaatg ctattgttga
     3181 aaccggcgtg cgtgtgccgt ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag
     3241 cgaagatgac gcggtgcggg cgcaggggct ggagattatg cgtaaagcca tccagttcgc
     3301 ccaggacgtt ggcattcgcg tgatccagct ggcgggctat gacgtttact atcaggaagc
     3361 caataacgaa acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg agatggcgag
     3421 ccgcgcgcag gtgacgctgg cgatggagat catggattat ccgttgatga actccatcag
     3481 caaggcgctg ggttacgcgc actatctcaa caacccgtgg ttccagctct acccggatat
     3541 cggcaacctg tcggcgtggg acaacgatgt gcagatggag ttgcaggccg gaatcgggca
     3601 tatcgtcgcg gtgcatgtga aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg
     3661 cgaaggtgtg gtggatttcg aacgttgctt cgaaacgctc aaacagagtg gctattgcgg
     3721 gccgtacctg attgagatgt ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa
     3781 agcgtgtgat tgggtgaaag cgcgcatggc caaagcaggc atggtggagg cggcataatg
     3841 caaaagctaa aacagcaggt gtttgaagcc aacatggatc tgccgcgcta tgggctggtg
     3901 acctttactt ggggcaacgt cagcgctatc gaccgcgaac gcgggctggt ggtgatcaag
     3961 cccagcggcg ttgcctacga aaccatgaaa gcagacgata tggtggtggt cgatatgagc
     4021 ggcaaggtgg tggaagggga gtatcgccca tcttccgaca ctgcgacgca tctcgaactc
     4081 taccgtcgtt acccgtcgct tggcggcatt gtccataccc actccactca tgccaccgca
     4141 tgggcgcagg cggggctggc gatcccggca ttaggcacca cgcacgctga ctacttcttt
     4201 ggtgatatcc cgtgtacgcg tggattaagc gaagaagagg tgcagggtga gtatgaactg
     4261 aacactggca aagtgattat cgaaacgctg ggcaacgccg agccgctgca tacgccggga
     4321 attgtggtgt atcagcacgg accgttcgcc tgggggaaag atgcccacga cgcggtgcat
     4381 aacgcggtag tgatggaaga agtggcgaaa atggcgtgga ttgcgcgcag tattaatcca
     4441 caactcaatc atatcgacag ctacctgatg aataaacact tcatgcgtaa gcacggtcct
     4501 aacgcttatt acgggcagaa gtaaaacacg cgctgcggaa atttccttcc tcgggagata
     4561 actggtctaa ttccgcagcc gtttttcaaa aaaagccccc tgcgaagggg gcaaagcaaa
     4621 ctatggcaat gtttcgttgg ttatacctgg tgctagcgat aaatatccgc gctggcgtgc
     4681 atattgccgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt tgcgccttgc
     4741 gcatctgttt cttcatttaa tgcctgacgt gcttcgcttt cagtggcgaa attatgattg
     4801 atataaatca cgcctaagct ttgcacatcg tccatatttc tggcctggtg gttattaatt
     4861 tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc taaaactcga
     4921 ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg atgatcttct
     4981 gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg cagttgttca
     5041 aaaaacactg tgacggaatg tgatgaactt caaatcagcg gtttagaggt tatttgcgaa
     5101 aggggagatt tatttctgct ccgcccttga gtttagcggg gtatgccagt actataacgc
     5161 gtcatttttc agccgacctt taacacgttc cttgcctccc cgggattcgg ctgacccaga
     5221 caggaggctg aataatccgt aaggagcaat tcgatgcgtc attacgaaat cgtttttatg
     5281 gtccatcctg accagagcga acaggttccg ggcatgatcg agcgctacac tgctgccatc
     5341 actggtgcag aaggcaagat ccaccgtctg gaagactggg gccgccgtca gctggcttac
     5401 ccgatcaaca aactgcacaa agcacactac gttctgatga acgttgaagc tccgcaggaa
     5461 gtgatcgatg agctggaaac taccttccgc ttcaacgatg ccgttatccg cagcatggtt
     5521 atgcgtacca agcacgctgt taccgaagca tctccgatgg ttaaagcgaa agacgagcgc
     5581 cgtgagcgtc gcgatgattt cgcaaacgaa accgctgatg atgctgaagc tggggattct
     5641 gaagagtaat ttctgatgac caaccgtctg gtgttgtccg gcaccgtgtg caggactccc
     5701 cttcgaaagg tcagtccatc aggaattcct cactgccagt tcgtgcttga gcatcgttct
     5761 gtgcaggagg aagccggctt tcaccggcag gcatggtgtc aaatgcccgt tattgttagc
     5821 ggacacgaaa accaggccat tactcacagt ataacggtcg gcagtcgcat aaccgttcag
     5881 gggttcattt catgccacaa ggcaaagaac ggactgagca aaatggtttt gcatgccgag
     5941 cagattgaat tgatagattc tggagactag ccatatggca cgttatttcc gtcgtcgcaa
     6001 gttctgccgt ttcaccgcgg aaggcgttca agagatcgac tataaagata tcgctacgct
     6061 gaaaaactac atcaccgaaa gcggtaagat tgtcccaagc cgtatcaccg gtacccgtgc
     6121 aaaataccag cgtcagctgg ctcgcgctat caaacgcgct cgctacctgt ccctgctgcc
     6181 gtacactgat cgccatcagt aatcggtcac ggtccattaa tacgactttg agaggataag
     6241 gtaatgcaag ttattctgct tgataaagta gcaaacctgg gtagcctggg tgatcaggta
     6301 aacgttaaag cgggctatgc tcgtaacttc ctggtaccgc agggtaaagc tgttccagct
     6361 accaagaaaa acattgaatt cttcgaagca cgtcgcgctg aactggaagc taaactggct
     6421 gaagttctgg cagctgctaa tgctcgcgct gagaaaatca atgcactgga aactgttacc
     6481 atcgcgtcta aagctggcga cgaaggtaaa ctgttcggtt ccatcggtac tcgcgacatc
     6541 gctgacgctg taactgcagc tggcgttgaa gtggctaaga gcgaagttcg tctgccgaac
     6601 ggcgttctgc gtaccactgg cgaacacgaa gtgagcttcc aggttcacag cgaagtattc
     6661 gcgaaagtga tcgtaaacgt agtagctgaa taattcgtta ttcaacgaga cgtaaaaacg
     6721 ccgaccattg gtcggcgttt tgctttctat ttttcgtcag gcattaattt cgcaagtaga
     6781 tccattcacc attctcttgt gtccataagt caggatggtt tgctggcgtt agatgcatac
     6841 gatcactcct tatgtctcgg tcctgcggca ctcgctcgaa acctaaaccc gagtagattg
     6901 cggctgaatt tgataaggaa cgtagaccaa ttttcaaatc atggggtaaa tcagaatacg
     6961 cgtttagcaa tgcagcaatg atgaatccga gggcattaga aacgcctgta acagtgtgaa
     7021 gttggtcgat ataaagatcg attgttggta gttcagatga gcagcaatta ggcatttggg
     7081 caggaagggt tacaacgtat cctatcgttt ggccggtata gtctggagta ctataaccag
     7141 gtcccaaaac aacctcgtgt attgtgtatt tactgttggg ttcgaaggat tcgacatctt
     7201 gcaataaata tttcagttct gcggttaacc tcgcacccgc gtaagcttta ttgtatttct
     7261 gggcgtgaat attatctaat gtagcttcat ggttccatcc aaaagaatta tcaccattca
     7321 ttgttactga atcgccacgg gtttaacaga cacctcagag tcatttaaga tgg
//
LOCUS       DAZPEU010000185         7232 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPEU010000185 DAZPEU010000000
VERSION     DAZPEU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.186"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHU2L_003435"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHU2L_003435"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR1358152.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHU2L_003436"
     CDS             complement(1672..2667)
                     /locus_tag="ACHU2L_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1358153.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHU2L_003437"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHU2L_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHU2L_003438"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHU2L_003438"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR1358154.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHU2L_003439"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHU2L_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR1358155.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..>7232)
                     /gene="emrK"
                     /locus_tag="ACHU2L_003440"
     CDS             complement(6108..>7232)
                     /gene="emrK"
                     /locus_tag="ACHU2L_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR1358156.1"
                     /translation="KKYFSLLVIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPI
                     SAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQD
                     KQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQA
                     YKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGYIAQRSVQVGETVS
                     SGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGT
                     GNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKDEDIAE
                     MPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         2000 a         1714 c         1666 g         1852 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tt
//
LOCUS       DAZPEU010000186         7183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPEU010000186 DAZPEU010000000
VERSION     DAZPEU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.187"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(371..1198)
                     /locus_tag="ACHU2L_003441"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACHU2L_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHU2L_003442"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHU2L_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR1358157.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHU2L_003443"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHU2L_003443"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR1358158.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHU2L_003444"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHU2L_003444"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR1358159.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHU2L_003445"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHU2L_003445"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR1358160.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHU2L_003446"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHU2L_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACHU2L_003447"
     CDS             7090..>7183
                     /locus_tag="ACHU2L_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR1358161.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAZPEU010000187         7105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPEU010000187 DAZPEU010000000
VERSION     DAZPEU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.188"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            309..1367
                     /gene="citC"
                     /locus_tag="ACHU2L_003448"
     CDS             309..1367
                     /gene="citC"
                     /locus_tag="ACHU2L_003448"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR1358162.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1382..1678
                     /gene="citD"
                     /locus_tag="ACHU2L_003449"
     CDS             1382..1678
                     /gene="citD"
                     /locus_tag="ACHU2L_003449"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR1358163.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1675..2583
                     /gene="citE"
                     /locus_tag="ACHU2L_003450"
     CDS             1675..2583
                     /gene="citE"
                     /locus_tag="ACHU2L_003450"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR1358164.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2594..4126
                     /gene="citF"
                     /locus_tag="ACHU2L_003451"
     CDS             2594..4126
                     /gene="citF"
                     /locus_tag="ACHU2L_003451"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR1358165.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4130..4680
                     /gene="citX"
                     /locus_tag="ACHU2L_003452"
                     /pseudo
     CDS             4130..4680
                     /gene="citX"
                     /locus_tag="ACHU2L_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4655..5533
                     /gene="citG"
                     /locus_tag="ACHU2L_003453"
     CDS             4655..5533
                     /gene="citG"
                     /locus_tag="ACHU2L_003453"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR1358166.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5584..7046
                     /gene="citT"
                     /locus_tag="ACHU2L_003454"
                     /pseudo
     CDS             5584..7046
                     /gene="citT"
                     /locus_tag="ACHU2L_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
BASE COUNT         1630 a         1919 c         1851 g         1705 t
ORIGIN      
        1 attaattatg atcgtggcaa attttattca ttaaatcaat taaatcactt aaacctgcga
       61 gtcatttcgt aattacatta attaaaaaac ttaaaaccat taaataaata aaaaaccact
      121 aactctatgt gaaataaatc aaaatttcac gccgaaatac tccttaggat gtatagcaaa
      181 aggagaaaaa gatatacctc gatcaccccc ctttctccca agtgaaaata aaaggttatc
      241 agtttgcaac attgaacaac attcgttgca aatcgataac aacatgcacc ttcaggatac
      301 tatttattat gttcggcaat gatattttca cccgcgtaaa acgttcagaa aataaaaaaa
      361 tggcggaaat cgcccaattc ctgcatgaaa atgatttgag cgttgacacc acagtcgaag
      421 tatttattac cgtaacccgc gatgaaaagc ttatcgcgtg cggtggaatt gccggaaata
      481 ttattaaatg cgttgctatc agtgaatccg ttcgcggtga aggactggcg ctgacattag
      541 ccactgaact gataaacctc gcctatgagc ggcacagcac gcatctgttt atttatacca
      601 aaaccgaata cgaggcgctg ttccgccagt gcggtttttc cacgctgacc tgcgtacccg
      661 gcgtgatggt gctgatggaa aacagcgcca cgcgactgaa acgctatgcc gaatcgctga
      721 aaaaatttcg tcatccaggg aacaagattg gctgcattgt gatgaacgcc aatcccttta
      781 cgaatggtca ccgttatctg attcaacaag ctgcggcaca gtgcgactgg ttgcatctgt
      841 ttttagtcaa agaagattct tcacgcttcc cctatgaaga ccggctggat ctggtgttaa
      901 aaggcaccgc cgatattcca cgcctgactg tgcatcgcgg ctcagaatac atcatctccc
      961 gcgctacgtt cccttgctac ttcattaaag aacagagcgt cattaaccat tgttacaccg
     1021 aaattgatct gaagattttc cgtcagtacc tcgctcccgc gcttggtgta actcaccgct
     1081 ttgtcggtac tgaacccttt tgtcgcgtta ccgcccagta caaccaggat atgcgctact
     1141 ggctggaaac gccgactatc tccgcagcgc ccatcgaact ggttgaaatt gagcggctgc
     1201 gttaccagga gatgccgata tccgcttccc gggtacgtca actgctggcg aaaaacgatc
     1261 tcacggctat cgcgccgctg gtccctgcag tcacgctgca ttatttgcag aacctgcttg
     1321 agcactcccg ccaggacgcg gcagctcgtc aaaagacccc cgcatgagaa acaggtgaaa
     1381 aatgaaaata aaccagcccg ccgttgcagg cacccttgag tctggggatg tgatgatacg
     1441 catcgcccca ctcgatacgc aggatatcga cctgcaaatc aatagcagcg ttgagaaaca
     1501 gtttggcgat gcaattcgca ccaccattct ggacgttctc gcccgctaca acgtgcgcgg
     1561 cgtacagctg aatgtcgatg acaaaggcgc actggactgc attttacatg cacgactgga
     1621 agccctgctg gcacgcgcca gcggtatccc ggctctgcca tgggaggatt gccaatgatt
     1681 tccgcttcgc tgcaacaacg taaaactcgc acccgccgca gcatgttgtt tgtgcctggt
     1741 gccaatgccg cgatggtcag caactccttc atctacccgg ctgatgccct gatgtttgac
     1801 ctcgaagact ccgtggcatt acgtgaaaaa gacaccgccc gccgcatggt ttatcacgcg
     1861 ctgcaacatc cgctgtatcg cgatattgaa accattgtgc gtgtcaacgc gctggattcc
     1921 gaatggggtg ttaacgacct ggaagccgtc gttcgcggtg gtgcggacgt tgtgcgtctg
     1981 ccgaaaaccg ataccgctca ggatgttctg gatattgaaa aagagatcct gcgtatagaa
     2041 aaagcctgtg gtcgtgaacc cggcagcacc ggcctgctgg cggcgattga atctccgctg
     2101 ggcattaccc gcgcagtgga aatcgctcac gcttccgagc gtttgatcgg tatcgccctc
     2161 ggtgcagaag actatgtgcg caacctgcgt acagaacgct ccccggaagg aactgaactg
     2221 ctgttcgcac gctgttccat tttgcaggcc gcgcgctctg cgggtattca ggcgttcgat
     2281 accgtctatt ccgacgctaa caacgaagcc ggattcctgc aagaagccgc ccacatcaaa
     2341 cagctgggct ttgacggcaa atcgctgatc aacccgcgtc agattgatct gctgcacaac
     2401 ctctacgcac cgacccagaa agaagtggat cacgcccacc gcgtcgtaga agccgctgaa
     2461 gccgccgctc gcgaaggcct cggcgtggtt tccctgaacg gcaagatggt ggacggtccg
     2521 gttatcgatc gcgcccgtct ggtgctctcc cgtgcagaac tttccggcat ccgcgaagaa
     2581 taaggcaatc aaaatgacgc agaaaattga acaatctcaa cgacaagaac gggtagcggc
     2641 ctggaatcgt cgcactgaat gcgatcttgc cgctttccag aactcaccaa agcaaaccta
     2701 ccaggctgaa aaagcgcgcg atcgcaaact gtgcgccaac ctggaagaag cgattcgtcg
     2761 ctcaggttta caggacggca tgacggtttc cttccatcac gctttccgtg gcggtgacct
     2821 gaccgtcaat atggtgatgg acgtcatcgc gaagatgggc tttaaaaacc tgaccctggc
     2881 gtccagctcc ctgagtgatt gccatgcgcc gctggtagaa cacattcgcc agggcgtggt
     2941 tacccgcatt tatacctctg gcctacgcgg tccactggcg gaagagatct cccgtggtct
     3001 gctggcggaa ccggtgcaga tccactctca cggcggtcgt gtgcatctgg tacagagcgg
     3061 cgaactgaat atcgacgtgg ctttcctcgg cgtcccgtcc tgtgatgaat tcggtaatgc
     3121 caacggctac accggtaaag cctgctgcgg ctccctcggc tatgcaatgg ttgatgccga
     3181 caacgcaaaa caggtcgtga tgcttaccga agaactgctg ccttatccgc ataatccggc
     3241 aagcattgag caagatcagg ttgatttgat cgtcaaagtt aatcgcgttg gcgatgctgc
     3301 aaaaatcggc gctggtacta cccgtatgac cactaacccg cgcgaactgc ttattgcccg
     3361 tagcgctgcg gatgtgattg tcaactctgg ctacttcaaa gaaggtttct ccatgcaaac
     3421 cggcaccggc ggcgcttcgc tggcggtaac ccgtttcctg gaagacaaaa tgcgtagccg
     3481 cgatattcgc gccgacttcg ccctcggcgg tattaccgcg acgatggttg atctgcacga
     3541 aaaaggtctg atccgcaaac tgctggatgt gcagagcttt gacagccatg ctgcgcaatc
     3601 gctggcccgt aaccccaatc acatcgaaat cagcgccaac cagtacgcta actggggttc
     3661 gaaaggcgca tcggttgatc gtctcgacgt ggtggtactg agcgcgctgg aaattgacag
     3721 gcagttcaac gttaacgtgc tgaccggttc tgacggcgta ctgcgtggcg cttccggtgg
     3781 tcactgcgat accgcgattg cctctgcgct ttccatcatc gtcgcgccac tggtacgcgg
     3841 tcgtattccg actctggtgg ataacgtgct gacctgcatc accccgggct ccagtgtcga
     3901 tattctggtc acagaccacg gtatcgcagt taacccggca cgtccggaac tagcagaacg
     3961 tctgcaggaa gcgggcatta aagtggtttc cattgagtgg ctgcgcgaac gtgcgcgtct
     4021 gctgaccggt gaaccacagc cgattgaatt caccgaccgc gtcgttgccg ttgtgcgtta
     4081 ccgcgatggc tcggtgatcg atgttgtgca tcaggtgaag gaataagcca tgcacctgct
     4141 tcctgaactc gccagccacc atgcggtgtc aattcccgag ctgctcgtca gccgggatga
     4201 aaggcaagca cggcaacacg cctggctcaa gcaccatcct gttccactgg tctcctttac
     4261 cgtggttgcg cctgggccga ttaaagacag cgaggtcaca cgccgaattt ttaatcatcg
     4321 cgtgacagcc ttgcgtgcct tagccacaaa gcagggctgg caaattcagg agcaggctgc
     4381 actggtttcc gccagcgggc cggagggcat gttgagcatt gccgccccgg ctcgcgacct
     4441 caagctcgcc accattgagc ttgaacatag tcatcctctc gggcggttat gggatatcga
     4501 tgtcctgacg cccgaaggcg aaattctctc ccgccgcgac tattcactgc gcctcgccgc
     4561 tgcctgttgt gcgagcaaag cgcagccgtc tgcgcgcgtg gaaaaaccca tcaactgacc
     4621 gatttactca accgcatgga ggcactgctg aacgatgtcg atgcctgcaa cgtcaactaa
     4681 aaccacaaag cttgcgacgt cattaatcga tgagtacgcc ctgctgggct ggcgcgccat
     4741 gctgactgaa gtcaatctgt caccgaaacc aggcctcgtg gatcgcatta actgcggtgc
     4801 gcacaaagat atggcgctgg aagatttcca ccgcagcgcg ctggcgattc agggctggtt
     4861 accccgtttc attgaatttg gtgcctgtag tgcagaaatg gcaccggaag cggtactcca
     4921 cggattacgc ccaattggta tggcttgcga aggtgatatg ttccgcgcca ctgcgggcgt
     4981 aaacacgcat aaaggcagca ttttttcttt agggctgcta tgtgcggcaa ttggccgttt
     5041 gcttcagctc aaccagccgg ttacgccaac aaccgtttgt tctacggcgg caagtttctg
     5101 ccgtggcctg accgatcgcg aactgcgtac caataattca cgactgacgg caggtcaacg
     5161 gttgtaccag cagcttggcc ttaccggcgc acgcggtgaa gccgaagcag gttatccact
     5221 ggtgatcaac cacgccttgc cgcattacct cactctgctg gatcaggggt tagatcctga
     5281 actggcattg ctcgatacct tgctcctact gatggctacc aacggcgata ccaacgttgc
     5341 atcgcgcggt ggcgaggggg gcctgcgctg gctacagcgc gaggcgcaaa cattattgca
     5401 aaaagggggc attcgaaccc ccaccgatct cgattatctc cggcagttcg acagggagtg
     5461 tatcgaacga aatctcagtc caggcggcag tgccgacctg ctgatcctta cctgtttttt
     5521 agcacagatt taattattta agcacttgat aaatttggaa atattaattt tcggagaacc
     5581 cgtatgtctt tagcaaaaga taatatatgg aaactattgg ccccactggt ggtgatgggt
     5641 gtcatgtttc ttatccctgt ccccgacggt atgccgccgc aggcatggca ttacttcgct
     5701 gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa ttccggcaac agcgatcagt
     5761 tttattgcgg ttactatttg cgttattggc agtaattacc tgctctttga tgccaaagaa
     5821 ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga aatggggcct ggctggtttt
     5881 tccagcacca cggtatggct ggtatttggc gcatttattt ttgcattagg gtatgaagtt
     5941 tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat tcatggacaa acgcacgctg
     6001 acgttgggtt atgcgattgt cattatcgac attctgctgg caccgtttac accgtccaac
     6061 accgcgcgta ccgggggtac ggtttttccg gtcattaaaa acctgccgcc gttgtttaaa
     6121 tcattcccga acgatccgtc cgcgcgtcgt attggcggct atttgatgtg gatgatggtc
     6181 attagtacca gtctgagttc gtccatgttt gtcaccggtg cggaccaaac gtgctgggtc
     6241 tggagttcgt cagcaaaatt gccggtatcc agattagctg gttgcagtgg ttcctctgct
     6301 tcctgccggt tggggttatc ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca
     6361 aaccggaaat cacacacagt gaagaagtgg caacctgggc gggtgatgaa ctaaaaacca
     6421 tgggtgcgct gacacgcaga gagtggacgc tgattggcct tgtattgctc agcttaggtt
     6481 tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt tggtctgctg gcagtttcgc
     6541 taatgctggc tctgcacgtt gtgccgtgga aagacattac ccgctataac agcgcatgga
     6601 acacgctggt caacctggca actctggttg tgatggctaa cggcctgact cgttctggtt
     6661 ttattgactg gttcgccggt accatgagta cgcacttgga aggattctca ccaaacgcaa
     6721 cggtgattgt actggttctg gtgttctact ttgcacacta cctgtttgcc agcctgtctg
     6781 cgcacaccgc aaccatgctg ccggttattc tggccgtcgg taaaggtatt ccgggcgtac
     6841 caatggaaca actgtgtatc ctgctggtgc tgtctatcgg tatcatgggc tgtctgacgc
     6901 cgtatgcaac cggtcctggg gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt
     6961 actggcgtct tggcgcaatc ttcggggtga tttacatctc tatgttgctg ttggttggct
     7021 ggccgattct cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc
     7081 cgccaggtaa gcccacctgg cgggc
//
LOCUS       DAZPEU010000188         6909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPEU010000188 DAZPEU010000000
VERSION     DAZPEU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.189"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..694
                     /locus_tag="ACHU2L_003455"
     CDS             <1..694
                     /locus_tag="ACHU2L_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1358167.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACHU2L_003456"
     CDS             complement(1033..1803)
                     /locus_tag="ACHU2L_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358168.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACHU2L_003457"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACHU2L_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACHU2L_003458"
     CDS             complement(2294..2515)
                     /locus_tag="ACHU2L_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358169.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACHU2L_003459"
     CDS             complement(2516..3199)
                     /locus_tag="ACHU2L_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR1358170.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACHU2L_003460"
     CDS             complement(3263..3574)
                     /locus_tag="ACHU2L_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358171.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACHU2L_003461"
     CDS             complement(3571..4473)
                     /locus_tag="ACHU2L_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR1358172.1"
                     /translation="MYFPSEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5871
                     /locus_tag="ACHU2L_003462"
                     /pseudo
     CDS             4913..5871
                     /locus_tag="ACHU2L_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
     gene            5871..6266
                     /locus_tag="ACHU2L_003463"
     CDS             5871..6266
                     /locus_tag="ACHU2L_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1358173.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6449..6829
                     /locus_tag="ACHU2L_003464"
     CDS             6449..6829
                     /locus_tag="ACHU2L_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR1358174.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1887 a         1785 c         1675 g         1562 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac taggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaataca cctatgattt gggaagcact
     5101 gctgtcatag gtacaacaca tgtcagttat caatacagca ccaccaatct gcttgccata
     5161 catcatgcac tgctgacatc cggccttcag ccacaggatg tagaactgac agtaacatta
     5221 cctgtgaccg aattctttga taatgacaat caaccaaatg aagaaagaat agaaagaaaa
     5281 aaggcaaatg ttctccgtga gatatctctg aataaagggg aaacatttaa aattaaaaaa
     5341 gttaatgtaa tgcctgaatc tttgccagct gcatttgaat cactaaaaaa agataaagta
     5401 aacaaacttg aacgcagcct gattatcgat cttggaggaa ccactctgga ttgtggttta
     5461 attctcggtg catttgaagg catttctgaa atccgtggct attcagaaat cgggacatcc
     5521 cgcatcactc ataccgtcat gaatgcgctg acgaaagcct ccacaccatg taattatttt
     5581 attgctgatg agttaataaa aaacagacac gataatgaat atctccagac attaataaat
     5641 gatgtagctg aaataaaaaa catttctcat gtaatagaca gagaagtgaa atctctggca
     5701 gaaagcatca ggcaggaaat ttcaacattc tcaggaatga accgaatcta tctcactggc
     5761 ggaggagccg agcttattta ccctcacata aagcaatatt tcccgaatct gaaagtgaac
     5821 aaggtggatg aacctcagtt tgcattggtg aaagcaatgg tgcatgcgta atggaaagca
     5881 gtgatccaaa aaaaagaaag aaagttgtgg catacctgca cccggcactt tatccgcagg
     5941 acaacctgac acaacaaaca atagactctc tcccagtaca aatgcgggga gatttctaca
     6001 gacaatccct tatttgcgga gcagctcttt attctgttgc cccccgactg ttaacgctaa
     6061 tcagcgtttt tttcagtgag aaaatcactg cagaaaatct ggtgaaactt atagaacaaa
     6121 caacaggata cacatccaca tcaattgata taagtgtgtt aaaaaacatc atagaggcat
     6181 cttcagaaaa caaatcagag agcatcactt caaaagatga tttcgaagaa caaaccagac
     6241 gcaatctttc tatgttaaaa aaataataaa agaagagcag gttcagtcaa ttacctgctc
     6301 ttcttttata catactaaaa aatcagattt ttacgaatat tcagattatc ataaaaaatg
     6361 caacagacag gaatgcaata attttgcatt atctcgttgc ttgtaatgca aatatgcatt
     6421 attgtcagtg tgtgacaata acagagagat gacgatggcg accattaacg cacgtattga
     6481 tgatgatata aagaaccaag cagatgaagt gctgaagctg atgaatatca gtcaaactca
     6541 ggctattgca gctttttatc agtatattac tgaacaaaag aaactgccat ttgtgatcac
     6601 ctcaattgta aaaacgccgc atgatttatt acgtgaaagt acagacatgt tagctgaagc
     6661 tcttgcagtg atttccaatc tacaggtgtg gactgaacag caggatggga ttggaaaagc
     6721 aaagctaatg gagtattacc gccgcctcga cgcactgtac tgttgtgcaa aagagaaaat
     6781 aggtcttctt tcagataacc gggatgctga actaggctgt gtcccttaat catctgagtt
     6841 atagtaactg tctgtttaca caacatattg aactatggct cgctacgacc ttcccgatga
     6901 ggcatggac
//
LOCUS       DAZPEU010000189         6722 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPEU010000189 DAZPEU010000000
VERSION     DAZPEU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.190"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..656
                     /gene="yneK"
                     /locus_tag="ACHU2L_003465"
     CDS             <1..656
                     /gene="yneK"
                     /locus_tag="ACHU2L_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR1358175.1"
                     /translation="DFIEQFPMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLV
                     LNFISRGNFVILPRLWNNSEVHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGS
                     NKHVQPEVCICFLSLLAEQESPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASL
                     SDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            806..1996
                     /gene="ydeA"
                     /locus_tag="ACHU2L_003466"
     CDS             806..1996
                     /gene="ydeA"
                     /locus_tag="ACHU2L_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR1358176.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2021..2686)
                     /gene="marC"
                     /locus_tag="ACHU2L_003467"
     CDS             complement(2021..2686)
                     /gene="marC"
                     /locus_tag="ACHU2L_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR1358177.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2898..3332
                     /gene="marR"
                     /locus_tag="ACHU2L_003468"
     CDS             2898..3332
                     /gene="marR"
                     /locus_tag="ACHU2L_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR1358178.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3352..3735
                     /gene="marA"
                     /locus_tag="ACHU2L_003469"
     CDS             3352..3735
                     /gene="marA"
                     /locus_tag="ACHU2L_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR1358179.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3767..3985
                     /gene="marB"
                     /locus_tag="ACHU2L_003470"
     CDS             3767..3985
                     /gene="marB"
                     /locus_tag="ACHU2L_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR1358180.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4016..4915)
                     /gene="eamA"
                     /locus_tag="ACHU2L_003471"
     CDS             complement(4016..4915)
                     /gene="eamA"
                     /locus_tag="ACHU2L_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR1358181.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5182..6297
                     /gene="ydeE"
                     /locus_tag="ACHU2L_003472"
                     /pseudo
     CDS             5182..6297
                     /gene="ydeE"
                     /locus_tag="ACHU2L_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            6424..6519
                     /gene="mgtS"
                     /locus_tag="ACHU2L_003473"
     CDS             6424..6519
                     /gene="mgtS"
                     /locus_tag="ACHU2L_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR1358182.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            6519..6622
                     /gene="mgtT"
                     /locus_tag="ACHU2L_003474"
                     /pseudo
     CDS             6519..6622
                     /gene="mgtT"
                     /locus_tag="ACHU2L_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         1729 a         1656 c         1551 g         1786 t
ORIGIN      
        1 atgattttat tgaacaattt ccaatgatca accttggcgc ccccatcaaa agagcgcata
       61 caccacatat tgaacccctg cctccggatc accataccgc tgcagattac ttacgccagt
      121 ttgatttgct tgtcctgaat tttatctctc gcggtaattt tgtcatactc ccccgattat
      181 ggaataactc tgaggttcac agatggttcg tcaataaaga tcctaacttg atcaccgcca
      241 ttctcgacat aacggacagc gaattaaaag aggatttatt gcaaagcctg atggattcat
      301 taggttctaa caaacatgta caacccgaag tctgcatctg cttcttatcc cttttagcag
      361 aacaagaatc tcctcatttt caggacttgt ttttattttt cgccaatatg ttattgcact
      421 atcaccaatt tatgaatccc aatgaaagtg atctgaatga cgtgttgatg ccagcatcat
      481 taagtgatga taaaattatc aaacatatgg cgcgcaggac cctcaaactg tttgtaaaaa
      541 atgaaacgcc gccaaaagtt actcacgaag acctggtgaa aaacaggcct cgctcccctg
      601 tcagaccacc tatacccgca accgccaaaa cgccagacct ccctgaacgt cattaaaccg
      661 tgatgttacc gactctctga ctcggaacag aatcagcgtc agagaaacgg aaaacgcgat
      721 ccagatcaca aatgcattgt attcacatca ttaaccgttt taagatcatt tcatcacttt
      781 ttcgcaactc acccgataat ctgttatgac aacaaacact gtttcccgca aagtggcgtg
      841 gctacgggtc gttacgctgg cagtcgccgc cttcatcttc aacaccaccg aatttgtccc
      901 tgttggcctg ctctctgaca ttgcgcaaag ttttcacatg caaaccgctc aggtcggcat
      961 catgttgacc atttacgcat gggtagtagc gctaatgtca ttgcctttta tgttaatgac
     1021 cagtcaggtt gaacggcgca aattactgat ctgcctgttt gtggtgttta ttgccagcca
     1081 cgtactgtcg tttttatcgt ggagctttac cgttctggtg atcagtcgca ttggtgtggc
     1141 ttttgcacat gcgattttct ggtcgattac ggcgtctctg gcgatccgta tggctccggc
     1201 cgggaagcgg gcacaggcct tgagtttaat tgccaccggt acagcactgg cgatggtctt
     1261 aggtttaccg ctcgggcgca ttgtggggca gtatttcggc tggcgaatga ccttcttcgc
     1321 gattggtatt ggggcgcttg ttaccctttt gtgcctgatt aagttacttc ccttactgcc
     1381 tagtgagcat tccggttcat tgaaaagcct cccgctattg ttccgccgcc cggcattgat
     1441 gagcatttat ttgttaactg tggtggttgt caccgcccat tacacggcat acagctatat
     1501 cgagcctttt gtgcaaaaca ttgcgggatt cagcgccaac tttgccacgg cattactgtt
     1561 attactcggt ggtgcgggca ttattggcag cgtgattttc ggtaaactgg gtaatcaata
     1621 tgcgtctgcg ttggtaagta cggcgattgc gctgttgctg gtgtgtctgg cactgctgct
     1681 acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt attttctggg ggatcgcgat
     1741 gatgatcatc gggcttggta tgcaggttaa agtgctggcg ctggcaccag atgctaccga
     1801 cgtcgcgatg gcgctattct ccggcatatt taatattgga atcggggcgg gtgcgttggt
     1861 aggtaatcag gtgagtttgc actggtcaat gtcgatgatt ggttatgtgg gcgcggtgcc
     1921 tgcttttgct gcgttaatat ggtcaatcat tatatttcgc cgctggccag tgacactcga
     1981 agaacagacg caatagttga aaggcccatt cgggcctttt ttaatggtac gttttaatga
     2041 tttccaggat gccgttaata ataaactgca cacccataca taccagcagg aaccccatca
     2101 ggcgggagat cgcttcaatg ccacccttgc ccaccagccg cataattgcg ccggagctgc
     2161 gtaggcttcc ccacaaaata accgccacca ggaaaaagat cagcggcggc gcaaccatca
     2221 gtacccaatc agcgaaggtt gaactctgac gcactgtgga cgccgagcta ataatcatcg
     2281 ctatggttcc cggaccggcg gtacttggca ttgccagtgg tacaaaggca atattggcac
     2341 tgggttcatc ttccagctct tccgacttgc ttttcgcctc cggtgaatca atcgctttct
     2401 gttgcggaaa aagcatccga aaaccgataa acgcgacgat taagccgcct gcaattcgca
     2461 gaccgggaat cgaaatgcca aatgtatcca tcaccagttg cccagcgtaa tacgccacca
     2521 tcatgatggc aaatacgtac accgaggcca tcaacgactg acgattacgt tcggcactgt
     2581 tcatgttgcc tgccaggcca agaaatagcg cgacagttgt taatgggtta gctaacggca
     2641 gcaacaccac cagccccagg ccaattgctt taaacaaatc taacattggt ggttgttatc
     2701 ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa caaggataaa gtgtcactct
     2761 ttagctaacc ttgcatcgca ttgaccaaaa cttgaaccga tttagcaaaa cgtggcatcg
     2821 gtcaattcat tcatttgact tatacttgcc tgggcaatat taccccctac aactaattac
     2881 ttgccaaggc aactaatgtg aaaagtacca gcgatctgtt caatgaaatt attccattgg
     2941 gtcgcttaat ccatatggtt aatcagaaga aagatcgtct gcttaacgag tatctgtctc
     3001 cgctggatat taccgcggca cagtttaagg tgctctgctc tatccgctgc gcggcgtgta
     3061 ttactccggt tgaactgaaa aaagtgttgt cggtcgacct gggagcactg acccgtatgc
     3121 tggatcgcct ggtctgtaaa ggctgggttg aaaggttgcc gaacccgaat gataagcgcg
     3181 gcgtactggt aaaacttacc accagcggcg cggcaatatg cgaacaatgc catcaattag
     3241 ttggccagga cctgcatcaa gaattaacaa aaaacctgac ggcggacgaa gttgcaacac
     3301 ttgagcattt gcttaagaaa gtcctgccgt aaacaaaaaa gaggtatgac gatgtccaga
     3361 cgcaatactg acgctattac cattcatagc attttggact ggatcgagga caacctggaa
     3421 tcgccactgt cactggagaa agtgtcagag cgttcgggtt actccaaatg gcacctgcaa
     3481 cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgcag ccgtaagatg
     3541 acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct ggcagaacga
     3601 tatggcttcg agtcgcaaca aactctgacc cgaaccttca aaaattactt tgatgttccg
     3661 ccgcataaat accggatgac caatatgcag ggcgaatcgc gctttttaca tccattaaat
     3721 cattacaaca gctagttgat aacgtgacaa cgtcactgag gcaatcatga aaccactttc
     3781 atccgcaata gcagctgcgc ttattctctt ttccgcgcag agcgttgcgg aacaaaccac
     3841 gcagccagtt gttacttctt gtgccaatgt cgtggttgtt cccccatcgc aggaacaacc
     3901 accgtttgat ttaaatcaca tgggtactgg cagtgataag tcggatgcgc tcggcgtgcc
     3961 ctattataat caacacgcta tgtagtttgt tctggccccg acatctcggg gcttattaac
     4021 ttcccacctt taccgcttta cgccaccgca agccaaatac attgatatac agcccggtca
     4081 taatgagcac cgcacctaaa aattgcagac ccgttaagcg ttcatccaac aatagtgccg
     4141 cacttgccag tccgactacg ggcaccagta acgataacgg tgcaacccgc caggtttcat
     4201 agcgtcccag taacgtcccc cagatcccat aaccaacaat tgtcgccaca aacgccagat
     4261 acatcagcga caagatggtg gtcatatcaa tagtaaccag actgtgaatc atggatgcgg
     4321 aaccatcgag aatcagcgag acaacaaaga agggaatgat tgggattaaa gcgctccaga
     4381 ttaccagcga catcaccgcc ggacgcgttg agtgcgacat gatcttttta ttgaagatgt
     4441 tgccacacgc ccaactaaat gctgcagcca gggtcaacat aaagccgagc atcgccacat
     4501 gctgaccgtt cagactatct tcgattaaca ccagtacgcc aaaaatcgct aaggcgatcc
     4561 ccgccaattg tttgccatgc agtcgctccc cgaaagtaaa cgcgccaagc acgatagtaa
     4621 aaaacgcctg tgcctgtaac accagcgaag ccagtccagc aggcatacca aagttaatgg
     4681 cgcagaacag aaaggcaaac tgcgcaaaac tgatggttaa tccatacccc aagagcaaat
     4741 tcagtggtac tttcggtcgt gcaacaaaaa agatggccgg aaaagcgacc agcataaagc
     4801 gcaaaccggc cagcatcagc ggtggcatgt tatgaagccc cactttgatg accacaaaat
     4861 ttagccccca tacgaccact accagtagcg ccaacacccc atcttttcgc gacattctac
     4921 cgcctctgaa tttcatcttt tgtaagcaat caacttagct gaatttactt ttctttaaca
     4981 gttgattcgt tagtcgccgg ttacgacggc attaatgcgc aaataagtcg ctatacttcg
     5041 gatttttgcc atgcgtccga tggttaataa caacgacgag gcaagaaggg cgctggtaga
     5101 gcgtcgtagg gataagttca tttgtctgtc cggcagtgcg tttcgttatg ttttatttta
     5161 gagatgtaaa gaaatagcat ggctacgctg ccatttatga ccatttactt gagtcgccag
     5221 tacagcctga gtgtcgatct aatcggttat gcgatgacaa ttgcgctcac tattggcgtc
     5281 gtttttagcc tcggttttgg tatcctggcg gataagttcg acaagaaacg ctatatgtta
     5341 ctggcaatta ccgccttcgc cagcggtttt attgccattc ctttagtgaa taacgtgacg
     5401 ctggttgtgc tcttttttgc cctcattaac tgcgcctatt ctgtttttgc taccgtgctg
     5461 aaagcctggt ttgccgacaa tctttcgtcc accagcaaaa cgaaaatctt ctcaatcaac
     5521 tacaccatgc taaacattgg ctggaccatc ggtccgccgc tcggcacgct gttggtgatg
     5581 cagagcatca atctgccctt ttggctggca gctatctgtt ccgcgtttcc catgcttttc
     5641 attcaaatat gggtaaagcg cagcgagaaa atcatcgcca cggaaacagg cagtgtctgg
     5701 tcgccgaaag ttttattaca agataaagca ctgttgtggt ttacctgctc tggttttctg
     5761 gcttcttttg taagcggcgc atttgcttca tgcatttcac aatatgtgat ggtgattgct
     5821 gatggggatt ttgccgaaaa ggtggtcgcg gttgttcttc cggtgaatgc tgccatggtg
     5881 gttacgttgc aatattccgt gggccgccga cttaacccag ctaacatccg cgcgctgatg
     5941 acagcaggca ccctctgttt cgtcatcgga ctggtcggtt ttattttttc cggcaacaac
     6001 ctgctattgt ggggtatgtc agctgcggta tttactgtcg gtgaaatcat ttatgcgccg
     6061 ggcgagtata tgttgattga ccatattgcg ccgccaggaa tgaaagccag ctatttttcc
     6121 gcccagtctt taggctggct tggtgccgcg attaacccat tagtgagtgg cgtagtgcta
     6181 accagcctgc cgccttcctc gctgtttgtc atcttagcgt tggtgatcat tgctgcgtgg
     6241 gtgctgatgt taaaagggat tcgtgcaaga ccgtgggggc agcccgcgct ttgttgattt
     6301 aagtcgaaca caataaaaat ttaattcagc cttcgtttag gttatctctg ctaatatctt
     6361 tctcattgag atgaaaatta aggtaagcga ggaaacacac cacaccataa acggaggcaa
     6421 ataatgctgg gtaatatgaa tgtttttatg gccgtactgg gaataatttt attttctggt
     6481 tttctggccg cgtatttcag ccacaaatgg gatgactaat gaacggagat aatccctcac
     6541 ctaaccggcc ccttgttaca gttgtgtaca aggggcctga tttttatgac ggcgaaaaaa
     6601 accgccagta aaccggcggt gaatgcttgc atggatagat ttgtgttttg cttttacgct
     6661 aacaggcatt ttcctgcact gataacggat cgttgacaca gtagcatcag ttttctcaat
     6721 ga
//
LOCUS       DAZPEU010000190         6675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPEU010000190 DAZPEU010000000
VERSION     DAZPEU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.191"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..430
                     /locus_tag="ACHU2L_003475"
     CDS             <1..430
                     /locus_tag="ACHU2L_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358183.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="ACHU2L_003476"
     CDS             550..723
                     /locus_tag="ACHU2L_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR1358184.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..1644
                     /locus_tag="ACHU2L_003477"
     CDS             823..1644
                     /locus_tag="ACHU2L_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR1358185.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            1986..2459
                     /locus_tag="ACHU2L_003478"
     CDS             1986..2459
                     /locus_tag="ACHU2L_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR1358186.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2475..2951
                     /locus_tag="ACHU2L_003479"
     CDS             2475..2951
                     /locus_tag="ACHU2L_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFR1358187.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3014..3235
                     /locus_tag="ACHU2L_003480"
     CDS             3014..3235
                     /locus_tag="ACHU2L_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR1358188.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3398..3772
                     /locus_tag="ACHU2L_003481"
     CDS             3398..3772
                     /locus_tag="ACHU2L_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFR1358189.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            3819..4193
                     /locus_tag="ACHU2L_003482"
     CDS             3819..4193
                     /locus_tag="ACHU2L_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR1358190.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            4190..4681
                     /locus_tag="ACHU2L_003483"
     CDS             4190..4681
                     /locus_tag="ACHU2L_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR1358191.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            4693..4890
                     /locus_tag="ACHU2L_003484"
     CDS             4693..4890
                     /locus_tag="ACHU2L_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR1358192.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            4975..5535
                     /locus_tag="ACHU2L_003485"
                     /pseudo
     CDS             4975..5535
                     /locus_tag="ACHU2L_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            5607..6611
                     /locus_tag="ACHU2L_003486"
     CDS             5607..6611
                     /locus_tag="ACHU2L_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFR1358193.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
BASE COUNT         1659 a         1762 c         1671 g         1583 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag
     1321 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
     1381 gcactgactt accgttatgg tgacgaacat cagccagtca ccaccgctga cattctgaca
     1441 ccacgacgca gggaggatta cggtaaagac ctgtggagcg catatcagac catccaggag
     1501 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
     1561 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt aatggcagaa
     1621 acgctgctgg agagcctgcg ctgaggcagc atgtccctga aggcgtgatt caggcaccag
     1681 aatcacccga cgccccggag gcgctgccgg ggtgtcgttc catttctgat gatgacctgt
     1741 gcgcctggtg tacccgtctg ctttaccgtc ccggagaaat cagtctgtgc agactgagta
     1801 tacaggcaat ggcatctggc catccgtctg tgaccggaac ggttacgcgt acggctgtcc
     1861 ggaatttcag ccgaatatca ttcatccctg atattaccgg gtcatcacgg tcagtgatga
     1921 acgtgatgtt gccagctccc gacctttact ccctctcatt tattcacatt acaaggattt
     1981 catacatgaa aacattgtct cagaacacca ccgcttcggc ctgtgcaccg gagactggcc
     2041 tgcaacaact ggttgccact cccgtccctg atgaacagcg catcagcttc tggccgcagc
     2101 attttggcct cattccacag tgggtcaccc tggaaccccg tgtcttcggc tggatggacc
     2161 gtctgtgtga agactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     2221 catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
     2281 tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg cattgctgcc tgtctgatga
     2341 cgtacagtca tcatgcctgt cgtacggagt gttatgccat gacggtccat tattaccggc
     2401 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     2461 tggaggcagg gacaatgcag cagctttcct ttctgcccgg aaacatgacg cccggcgagc
     2521 gcagtctcat tctgcgggcc ctgcaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     2581 ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg ggactggagc
     2641 gggaagagtt ccgggtgctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
     2701 tcttcaccgg caccatcaac cgtacggaag ttcatccccg ggaagtgatt aaacgcgccc
     2761 tgtaccacaa tgccgctgcc gtggtgctgg cgcacaatca cccgtccggt gaagtcacac
     2821 ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc ctggtggata
     2881 tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt gccgaacatg
     2941 gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca
     3001 gaggtatcac atcatgagaa tcatcacccg tggtgaagcc atgcgtattc accaacagca
     3061 tcctgcatcc cgtctttttc cgttctgtac cggtaagtat cgctggcacg gcagtgctga
     3121 agcgtatact ggtcgtgaag tgcaggatat tcccggcgtt cttgccgtgt ttgcagaacg
     3181 ccgtaaggac agttttggcc cgtatgtccg gctgatgagt gtcaccctga actgatgttc
     3241 cgtcacccgg gaaacgagta cgaccagatg acctcagacg ccgctgtgga tgaggccatt
     3301 ctcatcaatg catacatctt caccgaaacc ggtcagcgcg ccggctgacc tgtcatcgtt
     3361 aaaccattct taaccacttt tcagagagga ttttatcgtg tcagacaaac tctccgggat
     3421 aactcatccc gatgacaatc acgaccgccc ctggtggggg cttccctgca cagtgaggcc
     3481 ctgttttggc gctcgtctgg tgcaagaggg taaccgcctg tattaccttg ccgaccgcgc
     3541 cggtatcaga ggccgcttca gcgacgcaga tgcataccat ctggaccagg cctttccgct
     3601 gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc gatatcagca
     3661 taccgtcacg ctgtatgcaa aagggctgac ctgcgaagcc gacaccctcg gctcctgtgg
     3721 ttacgtttat ctggctgttt atccgacacc agcacccgca accacctcat aaccccgaag
     3781 ctctcacctt tagcactacc agttacagag aacttaagat gaaaacatta tccgacacac
     3841 atgtacggga ggtatctcgc tgcccgtctc ccgtcaccat ctggcagaca ctgctcatcc
     3901 gactgctgga ccagcattat ggcctcacac tgaatgacac accgtttgtc gatgaacgtg
     3961 tgattgagca gcatattgag gcaggcattt cactgtgtga tgcagttaat tttctcgtgg
     4021 aaaaatacgc gctggtgcgt accgaccagc cgggattcag cacctgtccc cgctctcagt
     4081 taataaacag tattgatatc ctccgggctc gccgggcgac cggcctgatg acccgcgaca
     4141 attacagaac ggtaaataac attaccctgg gtaagtatcc ggaggcgaaa tgatgaaact
     4201 ggccctgaca ctggaagccg acagcgttaa cgtacaggca ctgaacatag ggcgcattgt
     4261 cgttgacgtc gatggtattg agctcgctga actgattaac atggtctgcg ataacggcta
     4321 ctcccttcgt gttgttgatg aatctgacca gacctcagca gaatgcacgc caccatttgc
     4381 tacccttacc ggcatacgct gcagtaccgc acatatcacg gaaacggaca acgcctggtt
     4441 gtactcgctg tcacaccaga ccagtgactt cggtgaatca gaatggattc attttacagg
     4501 tagcggatat ctgttacgta ccgatgcgtg gtcatacccg gttctgcggc ttaaacgcct
     4561 ggggctgtca aaaacgttcc gtcgtctggt cgtcacactc atccggcgtt atggcgtcag
     4621 tctcattcat ctggatgcca gcgctgaatg cctgccgggt ttacccactt tcgactggta
     4681 accaggaaca acatgaaatc attaaccacg gaaacagcgc tggatattct gattgcgtgg
     4741 ctgcaggaca atatcgactg cggatcggga attatctttg acaacgatga ggataaaacg
     4801 gattcagcag cactgttgcc ctgtatcgaa caggcccggg aggatgtccg taccctgcgt
     4861 catctgcagc ttctgcacca gaaccggtga agctcactca ccagacttca ttcctctgac
     4921 gccagcctgc acagggctgg cgttttcatt tatctgcaaa aaggaatatc gattatgtca
     4981 gatatcacaa tctcccgtcc ggaagtggtc aacgggcata cggacgttat ctgctcaacc
     5041 agcattcgcc acattctggc tgtacgaaag agtacactgt tgcaaatcga cacacttatc
     5101 cgacaactgg ctgaaatctc ggtgctgacg gaaagcatcg gaggtaaaac tgccccggac
     5161 tgggccatga aacaggattt tcgctgcggc tgctggctga tggagaaacc cgaaacagca
     5221 atgaaagcca ttacccggaa tcttgatcgc gaaatctggc gtgacctgat gcaacgttcc
     5281 gggatgctgt cattaatgga tacgcaggcc cgtgatacat ggtaccggtc actggagtac
     5341 gataattttc cggaaatcag cgaagcgaac attctgagca catttgaaca actgcaccag
     5401 aataaggatg aggtgtttga gcgaggggtg atcaacgtct tcagagggct gaactggaat
     5461 tacaaaacca attgcccctg taaatttggc agtaaaatta tcgtcaacaa tctggtgaga
     5521 tgggaccggt ggggataatg atatggtcac ccccacctta acagcagtac gctgttaagg
     5581 taaatccctt taacggagag accatcatgc agaacatcac acttattggt atcgatctcg
     5641 gaaagcactc cttccacatt cattgtcaag acaagtctgg taaggctctt ctgcgtaaaa
     5701 aatttacccg tactaaactg atgcagtttc tggcgacatg tccgccctca gttgttgtaa
     5761 tggaagcctg tgcgggtgca cactttatgg ctcgctgtat ctctgatttt gggcatgaaa
     5821 ctaagctgat ttctccacag ttcgttcgcc cgtttgtcaa aagtaataag aatgattttg
     5881 ttgatgcaga agccatatgc gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
     5941 gaactgagac ccagcaggcc atgagggcgt tacaccgggt cagagaatcg ctcatcagag
     6001 ataaggtgaa aacaaccaac cagatccacg gtttcttgct ggaatttgga atcagcctgc
     6061 cgataggtga tgctgtaata aaacggttgt ctctcgtgtt ggctgaacac gaaatccccg
     6121 aatatctgag ccgccttttg gtgaggttgc atacccacta cttttacctt gttgagcaaa
     6181 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
     6241 ttatgacaat tcctggtgtt ggtccgatta ctgccagctt actatcgtcg caacttggtg
     6301 acgggaaaca gttctcatgc agcagggatt ttgcagcttc cacaggactt gttccccggc
     6361 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
     6421 tacgccggtt actggttcag tgtgcccgct cattcataat gcgacttgaa cgcaagcatg
     6481 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttacct
     6541 gcgctctggc aaataaactg gctcgaattg cctgggcaat cactactcag aaaaaagaat
     6601 atcaggcctg atatagatgc tgatataact caattagttt attttaaaca ataagataca
     6661 agttaccttt ctggt
//
LOCUS       DAZPEU010000191         6596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPEU010000191 DAZPEU010000000
VERSION     DAZPEU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.192"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1075
                     /locus_tag="ACHU2L_003487"
     CDS             <1..1075
                     /locus_tag="ACHU2L_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFR1358194.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1118..1621
                     /gene="yeaK"
                     /locus_tag="ACHU2L_003488"
     CDS             1118..1621
                     /gene="yeaK"
                     /locus_tag="ACHU2L_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR1358195.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1622..1726)
                     /gene="yoaI"
                     /locus_tag="ACHU2L_003489"
     CDS             complement(1622..1726)
                     /gene="yoaI"
                     /locus_tag="ACHU2L_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR1358196.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1896..2342
                     /locus_tag="ACHU2L_003490"
     CDS             1896..2342
                     /locus_tag="ACHU2L_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR1358197.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2299..3117)
                     /gene="nimR"
                     /locus_tag="ACHU2L_003491"
                     /pseudo
     CDS             complement(2299..3117)
                     /gene="nimR"
                     /locus_tag="ACHU2L_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3214..4394
                     /gene="nimT"
                     /locus_tag="ACHU2L_003492"
                     /pseudo
     CDS             3214..4394
                     /gene="nimT"
                     /locus_tag="ACHU2L_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4449..4796
                     /locus_tag="ACHU2L_003493"
     CDS             4449..4796
                     /locus_tag="ACHU2L_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR1358198.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4818..5072)
                     /gene="yoaF"
                     /locus_tag="ACHU2L_003494"
     CDS             complement(4818..5072)
                     /gene="yoaF"
                     /locus_tag="ACHU2L_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR1358199.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5255..6280
                     /gene="dgcP"
                     /locus_tag="ACHU2L_003495"
     CDS             5255..6280
                     /gene="dgcP"
                     /locus_tag="ACHU2L_003495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR1358200.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6313..6411
                     /locus_tag="ACHU2L_003496"
     CDS             6313..6411
                     /locus_tag="ACHU2L_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR1358201.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6414..6488
                     /gene="yoaJ"
                     /locus_tag="ACHU2L_003497"
     CDS             6414..6488
                     /gene="yoaJ"
                     /locus_tag="ACHU2L_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR1358202.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1599 a         1598 c         1615 g         1784 t
ORIGIN      
        1 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
       61 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
      121 actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
      181 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
      241 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
      301 gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt caggaagtat
      361 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat
      421 ttaccaggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc
      481 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatttgc ggaatatctt
      541 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgataccga
      601 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt atgtcagtta
      661 cgtccatgac ttacctggcg gcattcgtgt ctcgttatcc attgatattc tttactttat
      721 cacgtcttcg aggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
      781 ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
      841 gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact
      901 ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagcaaat
      961 aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttacatg atgcgtataa
     1021 agcttccgat gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc
     1081 ttctgtggtt gtttgcttgt aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag
     1141 cgtgacgcat cagcagttaa tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt
     1201 tacccatgaa gcggtaggta aatgtgaagc ggtgtcagaa attcgtggta ccgctctagg
     1261 ccagggtgct aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct
     1321 ggcgatcctc gcagccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg
     1381 attacgcgct tcgttggcca gcccggcgga agttgatgaa ctcacaggct gtgtcttcgg
     1441 cgcgatcccc cctttcagct tccatccaaa actcaaactg gttgccgacc ctctactctt
     1501 cgaacgattt gatgaaatcg ccttcaatgc agggatgctg gataaatccg ttattttgaa
     1561 aaccgcggat tatttgcgca ttgcgcaacc agaactggtc aatttccgcc gcactgcgta
     1621 gctagcctat ccgttcaata agcagaacgg acaaaaccag cacaaccgca atagcaaaaa
     1681 acgatgatgt gataatcagt gtctcgacga acatttgatc gttcatagca tgcccccggg
     1741 agatagctgt tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc
     1801 tgatttttta tgcaaaaaac gtaaagattt tctacttcct tcctgcagca agcataaagt
     1861 aagcaggctt attatttttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc
     1921 tgctcgggtt agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc
     1981 tggtgttgat cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac
     2041 aggggctaag tatcgggata atcattctga cgattggcgt tatggccccc atcgccagcg
     2101 ggacgctacc gccctcaacc ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa
     2161 ttgcagtagg cgttattgtc tcctggctgg gtgggcgcgg cgtgacgtta atgggcagcc
     2221 agccgcaact ggttgctggg ctgctggtgg gcactgtttt aggcgtagcg ctgtttcgcg
     2281 gcgtaccggt tggcccgctt attgccgcgg gtctggtttc gctgattgtg gggaaacagt
     2341 agttaatccc gcaatatatc ggcctggcgt ttgccccaag cctttcttaa acatagtaat
     2401 aaaggccgtt gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt
     2461 cactaatccc tgaagcgcca taatcagctg taactgctga cgccactggc gaaaactcaa
     2521 ccccgtctct ttcactatca ggcgggcaag attgcgttca ctcatcgcaa aaaagccagc
     2581 ccattgcccc aatgcccccc actcgacggg ccctttcgcc atcatctcca ccatcgtgcg
     2641 gattttagga tgagaagaaa cgggcaggtg cagctgttgt tgtggctgct gagggagttc
     2701 atcaaacagg acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct
     2761 gtttgccagg gttaaaatca attcccggca taagggcgat attttcaatg tgcaacatgg
     2821 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     2881 cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     2941 acaaagcatt ttccaccgta caggtaattg caccatgtaa tgccagtatc agctgtcctt
     3001 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     3061 ccgcggcttc atggtgtcgg tgggttcata gccattgaga ttcaacctgt gcatcatttt
     3121 gtccgaactt agcgataatt tgtcatttta gcttgattca acataacaat aaaaacggta
     3181 aggtacagcc tcgtttgtaa caatgagaag catatgacct gttcaacttc attaagcggc
     3241 aaaaacagga ttgtccttat cgctggcatt ctgatgattg ccacaacatt acgcgtcacc
     3301 tttaccggcg cagcgccgtt actggatacg attcgttccg cttactcgct gacgacagcg
     3361 caaaccggct tattaaccac cctgccatta ttggcctttg cgctaatctc acctctggct
     3421 gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt acttttgatc
     3481 tgtgcaggta tcgcaattcg ttctctccct tcgccttact tattatttgg cggtacagcg
     3541 gtcattggcg gtgggattgc attaggcaat gtcttactgc caggattaat taaacgcgat
     3601 ttccctcatt ccgtcgccag acttaccggc gcatattccc tgacaatggg agctgcagcg
     3661 gcactgggat cggctatggt cgtgccgctg gctttgaacg gttttggctg gcaaggcgcg
     3721 ttgttcatgc tgatgtgttt tcctctgctg gctctttttt tatggctgcc acagtggcga
     3781 agtcagcaac atgcaaattt gagtacctca cgcgccttac atactcgggg tatctggcgt
     3841 tcgccacttg cctggcaggt aacattgttt cttgggatca actcactggt ctattacgtg
     3901 attattggct ggcttccggc gatcctcatc agtcacggct atagcgaagc acaggcgggt
     3961 tcactgcatg gtttgctgca actagccaca gcagctcccg gtttgctgat cgcacttttc
     4021 ttacatcatg tgaaagacca gcgtggtatt gcagcgttcg ttgccttgat gtgcgcagtg
     4081 ggcgcggttg ggctctgctt tatgccagcg cacgcgatca cctggactct gcttttcggt
     4141 tttggttccg gcgcaacaat gatactgggg ttgacgttca ttggtcttcg gcaagttctg
     4201 cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct
     4261 gtgggccgcc gctaatgggt aaaatacacg atgctaacgg taactggtcc gtaccactga
     4321 tgggtgttgc aatactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca
     4381 aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcttgcgtg aaagggagaa
     4441 caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt
     4501 atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg
     4561 atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt cacggcgaag
     4621 tcgtcgattt tgcaaccttt cgcgagcaat atcgcgcaga actggcgcaa cacgagcaag
     4681 aaggaaagcg gctggcggac atcgccaaaa aacagccgct gaccctgctc tacgcagcaa
     4741 aaaacaccac gcagaaccat gcgctggtgc tggccgactg gctacgtagc ttgtgatttt
     4801 agtacggcat ccggcggtta tttttcacca gccggatggt cccgtcgcca caatgcccat
     4861 tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg cgctgtctgc
     4921 actggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt tgccatacca
     4981 atctgcggcg gtttaggtgc ttctggctga gaaggggttg aacaaccagc caggaccagc
     5041 aagcaaggca gaacaaaact gataattttc atttattgat ctcacatatt tatccaagct
     5101 tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc actcaaaaca
     5161 tattacgtct tgtttcatct ttgttgatga tgttttatca tgcctgcaaa gattaaataa
     5221 tcagcactta cccgccgtat cctggagttg ttccgtgtca gatcagatta tcgcccgcgt
     5281 ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg gtccgacagc ttctggagat
     5341 gctggaaatg gtcactgata tggaatcaac ctacctgacc aaagtggatg tcgaagcgcg
     5401 cctgcagcat attatgtttg cccgtaacag ccagaaaatg cacatcccgg agaattttac
     5461 cgtcttgtgg gattactcgt tatgcaaacg cgccattgat gaaaactgct ttttcagcga
     5521 tgaagtccct gaccgttggg gcgactgtat tgcggcacgc aatcttggca tcaccacatt
     5581 tctgagcacg ccaattcact taccggatgg atcattctat ggcacgcttt gcgccgccag
     5641 cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt ttacagttat tcgccggact
     5701 gattgcacaa tatattcaaa aagaggcact ggttgaacag ctgcacgaag ccaatgctgc
     5761 gctgattgcg caatcgtata ccgactcgtt aaccgggcta ccgaatcggc gggcgatttt
     5821 tgaaaatctg acgacgctgt tttccctcgc ccggcatctt aaccataaga taatgatcgc
     5881 gtttatcgat ctggataact tcaaattaat caatgatcgt tttggtcata atagtggcga
     5941 tctgtttctc attcaggttg gcgagcgcct taatacgctc cagcaaaatg gcgaagttat
     6001 tggtcgtctc ggtggtgatg agtttttggt tgtttcacta aacaacgaga atgcggatat
     6061 ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga gaatatcact taggtgatgt
     6121 tgatttgtat tatcccggtg ccagtcttgg catagtagaa gtcgatcctg aaacaaccga
     6181 tgcagacagt gccctgcatg ctgccgatat tgcgatgtat caggagaaaa aacacaaaca
     6241 gaaaacacct tttgtcgcgc atccagcgct acattcctga ggcgtattca catccttttg
     6301 attggtgata acatgcgaat cggtattatt tttccggttg taatcttcat tacagcggtc
     6361 gtatttttag catggttttt tattggctgc tatgctgccc cgggagcata aagatgaaaa
     6421 aaacaacgat tattatgatg ggtgtggcga ttattgtcgt actcggcact gagctgggat
     6481 ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt
     6541 taagcgttaa cttttaatct tcctgtagat aaatagcacg acaatcgcac caataa
//
LOCUS       DAZPEU010000192         6562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPEU010000192 DAZPEU010000000
VERSION     DAZPEU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.193"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..251
                     /locus_tag="ACHU2L_003498"
     CDS             <1..251
                     /locus_tag="ACHU2L_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358203.1"
                     /translation="FFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPI
                     KEMFWNLQGDLNLTCWFHYKDGQWRVITYIFWPPGARF"
     gene            276..2489
                     /locus_tag="ACHU2L_003499"
     CDS             276..2489
                     /locus_tag="ACHU2L_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR1358204.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            2512..2957
                     /locus_tag="ACHU2L_003500"
                     /pseudo
     CDS             2512..2957
                     /locus_tag="ACHU2L_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            2979..6562
                     /locus_tag="ACHU2L_003501"
                     /pseudo
     CDS             2979..6562
                     /locus_tag="ACHU2L_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601611.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1583 a         1641 c         1998 g         1340 t
ORIGIN      
        1 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
       61 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      121 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      181 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
      241 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
      301 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
      361 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
      421 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
      481 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
      541 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
      601 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
      661 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
      721 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
      781 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
      841 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
      901 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
      961 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1021 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1081 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1141 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1201 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     1261 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     1321 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     1381 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     1441 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     1501 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     1561 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     1621 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     1681 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     1741 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     1801 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     1861 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     1921 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     1981 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2041 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2101 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2161 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     2221 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     2281 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     2341 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     2401 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     2461 cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     2521 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     2581 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     2641 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     2701 aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg
     2761 aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt
     2821 accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca
     2881 ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc
     2941 agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg
     3001 atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta
     3061 tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga
     3121 ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg
     3181 ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca
     3241 acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg
     3301 aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga
     3361 tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg
     3421 cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt
     3481 ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc
     3541 ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat
     3601 ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac
     3661 cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt
     3721 cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg
     3781 gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt
     3841 acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga
     3901 cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac
     3961 cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt
     4021 tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg
     4081 ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga
     4141 acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg
     4201 ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca
     4261 gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac
     4321 ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg
     4381 cgaggattac cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc
     4441 ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc
     4501 gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact
     4561 ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac
     4621 ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg
     4681 ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat
     4741 acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc
     4801 ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt
     4861 actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg
     4921 gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga
     4981 agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg
     5041 cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa
     5101 tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga
     5161 acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg
     5221 gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga
     5281 aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg
     5341 gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg
     5401 caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag
     5461 cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag
     5521 cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc
     5581 cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc
     5641 ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac
     5701 ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc
     5761 agcggctggc tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac
     5821 aaagcgggca acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg
     5881 ggttacgggc gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac
     5941 cagcagctgg agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag
     6001 acgcggtatc agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat
     6061 accgaccggg gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag
     6121 gagacaacgt ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg
     6181 gtggctgcca tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg
     6241 acgggcacgg tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag
     6301 gcgggatttg gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg
     6361 cagcggctgc cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg
     6421 tattatgcgc cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg
     6481 ttgaacttat atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat
     6541 tctttaggaa tagattcaaa ta
//
LOCUS       DAZPEU010000193         6532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPEU010000193 DAZPEU010000000
VERSION     DAZPEU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.194"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHU2L_003502"
     CDS             complement(<1..353)
                     /locus_tag="ACHU2L_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1358205.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
     gene            complement(350..934)
                     /locus_tag="ACHU2L_003503"
     CDS             complement(350..934)
                     /locus_tag="ACHU2L_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1358206.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(921..1298)
                     /locus_tag="ACHU2L_003504"
     CDS             complement(921..1298)
                     /locus_tag="ACHU2L_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1358207.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            complement(1310..1696)
                     /gene="gpFI"
                     /locus_tag="ACHU2L_003505"
     CDS             complement(1310..1696)
                     /gene="gpFI"
                     /locus_tag="ACHU2L_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1358208.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            complement(1748..2776)
                     /locus_tag="ACHU2L_003506"
     CDS             complement(1748..2776)
                     /locus_tag="ACHU2L_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1358209.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(2834..3181)
                     /locus_tag="ACHU2L_003507"
     CDS             complement(2834..3181)
                     /locus_tag="ACHU2L_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1358210.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            complement(3218..4723)
                     /locus_tag="ACHU2L_003508"
     CDS             complement(3218..4723)
                     /locus_tag="ACHU2L_003508"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1358211.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(4713..6306)
                     /locus_tag="ACHU2L_003509"
                     /pseudo
     CDS             complement(4713..6306)
                     /locus_tag="ACHU2L_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6303..6506)
                     /locus_tag="ACHU2L_003510"
     CDS             complement(6303..6506)
                     /locus_tag="ACHU2L_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR1358212.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
BASE COUNT         1420 a         2069 c         1574 g         1469 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtggga tcatggtgtc gatgagtccg
       61 gaaagcaccg gaatatcctt catggccgga aatatggtgt tctccatcca cgtatccagc
      121 tcagaatccg gcgcctgtgc acggacgaaa acggcaatat gcagaacagc ctgccagtcg
      181 tcctcatccg tcatttttcc ggtgtactgc gcatcactca gccacaccgc cacggcgggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcgtgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacatc gggattttca
      481 ccacatcaat ggggtaacga tttttgccgt caatacgccg catcacatgc cagcgaccat
      541 tcgccagttg ctgaataaac gcatcccgga aaaggtattt ccccactttc agtacgctgc
      601 cacgatgacg tgagttgctg ccatgccggg agcgggccag tctgacctgt gcggtaccca
      661 gcttaatggc gggcaggtta ccccggttaa cacgcatgcg ggcatacatt tttcctgagg
      721 ggctggcttt aagcagcctg acacgctccc tgaccgtttt caggggaata cctttcacct
      781 ggttatcccc ggcaacggta ttctgcgcaa cctgccgtgt ggcgacagaa acaatctttg
      841 ccgccacacg gtttaccgcc catgcgctgg tctgtggcac catgcgggta tcgaggctgt
      901 tcagattgcg gatggcattc tcaagtccct tcatcatatc gcctcattct ggcattattc
      961 tgtggggcag gactgccccg ccttaaccgg atataacagc atcccccgtc atccggagta
     1021 atacgatcca caaagaaaga atcatccccg atagttaacg tatcctgacg gcgtaactgc
     1081 gatatatctg ccgttttcac aaacaggcac ggtgaggaat cctcaagccg gatccccccg
     1141 gcaacaaaag aaatactttc gggatcatca aaaacccctg tcaggtgtgt cccctcaagt
     1201 tctccggacg taatgacggc gctgatcccc atatcacgga gaatgacttc attgacaccg
     1261 gcaagcgcag catcaaacag attatcgaaa ccgtccacga catctcctgt tactgccttt
     1321 ttgccagacc acctgccgcc atgctggcag ccacctcagc agaaacacga aacgacgttc
     1381 ccggtgaaac aaatatcact gaaccattac attttgtatg cagtgtcgcc agtgccacca
     1441 ccgtaacctg ccccgtttcg tctccggtaa caatgcctgg ctgttcagga actgactcgc
     1501 ctttatgcgc ggtcggatca tctttcgatg gtgagtacgt atccggctct gcgacctccc
     1561 ccgcatctcc aagctcttcc tccagttccg ccactcgctg cgcaatatct gccgcactac
     1621 cggagacatc cggttcacgc cccagcctct cagccagctc acaaagacgt ttcaggtttt
     1681 cttctttcgt agccataccc gctcctgatt gaatgaaaaa cgggagcatt tagctcccgc
     1741 tcacggatta tttcacctgc accaccacaa actcatccgg atccggcagc accatcagcg
     1801 gcgcggactg cgtcatggtg aattcacgcg ccggatcacc cacggtcagc cagtgtttcg
     1861 gataacggga agacgccacc acgccttcag acaatgcctg cgcatcctga atggcaccgt
     1921 agcaacgaat accctctgcg gccgtatttc ccagtaccag tgtgccgtcc ggcagataac
     1981 gtttttcggt accgtcctct gccacatagg acgtttttgc caccacgatt gccagatcac
     2041 cataataccc tttgaaggac accaccgccc ccaggtcttt cactgccgtt tccagctgtg
     2101 aatttgagcc acgacgggta tccagttttt cgcggaacag cttaaagcca ttcagcagac
     2161 gccagacctt accatccata atggcaatat tgatggtgcc ggaagcaaaa tcacagtacg
     2221 catccagatc atgcgtcgga tcaaaggtat cgcggtcctg agcgctccac tccgtccctc
     2281 ctgcctgcgt aatgttattt cccaccgacc ggccaaaatc gacctcaaca gtttcaaact
     2341 gctctccttc catggtgtat ttgccataca gcacggcatt caccgcctgc atctcttcca
     2401 cctggacaat ggcgtgctct tcctgtttga ggttatcggt gatgatacgc aggcgacggt
     2461 aagccgggcc gttcagctgg gccggatctt caccgggaag acgctccacc gcctgctggt
     2521 aattaaattc gtgtttcggc ttaacgtagc ccgggcgcag tacacgagtt tcaccgccac
     2581 ggtgacgcaa cacctttcct tcaacaaccg gggagacata ggccgccacc ggcgtttttc
     2641 cggtaatttt gtccagcatt acctcttcgg tatggaaatt caccgtacgg cggaaaaaca
     2701 gctccagaaa cagcgcacga aatttaactt tttgttcggt ataaccgagt aactggcggg
     2761 tcgtaaacaa tcccataaat cagttccttt cattcagaaa tcagtcaggc taccgcagtg
     2821 acctgataaa atgttacggc agcgctgcgt gactcagggc actgccggca aaggcattcg
     2881 cctttttgtg tgtatccact ctctcaggcc agtggatggc ctccgtcgca aaggtccccg
     2941 acttgtaata cgtcagcacc gtctctgtac cttcaagcgg cagtaccagt atgccaacca
     3001 catggcctgc cttctgtccg tcccagacca ccagtttccc ggtatcctca tccagcatca
     3061 ggggcgtcag agccggtgtt gccgaggaaa tcccgctgca gcctgtggcg gtatgggccg
     3121 gatcattacc ggcaaaaata cgtacttccg cacgctgttc agtgatggtt ttcgttacca
     3181 tattgtaaaa acctcatatt gatggtcagc actgacttca tggcatggcc atgagcattt
     3241 tcacgtccgc atcaccgtct gctgacgtct gtggcacgcc accctgtacc gctgccggtg
     3301 aatggttcgc catgaaatgt tcaaacatgg cggttgtgga tgcagaaacc ggttctgccc
     3361 tgcctgaggc tgccgccagt acagcccggg cattctccac ggtcatcccc ggacaggccg
     3421 ccagtttttc agcctgcgcc tctgcccctt ttgcctcatc cagggccata atctgatcac
     3481 gaagtgaggg tccggcattc gcctgcggtg aagcagccag aatcggacgg gctttttcca
     3541 ccgtcatctc cggcatcgcc gccagcgttg ccgccagttg ttcacgtcct ttcgcctctt
     3601 cacatgccat aatgcgatcg gcttcactct gcgcagatac accagattgt tgcggtgccg
     3661 ccacggccag aatcgcccgg gcctgctcaa cgctcatacc ctgttgccct gccagcatcg
     3721 tggccagttg ttcacgtcct ttcgcttcct gacacgtcag gatccccatc actcgctggt
     3781 tctcctgcgc ggcagcatcc gttgcagtta attgcggcat agtgcctcct gtatcgtgtg
     3841 tgttcagcgt cgcagccatc acgctgatgg cgtccgacgc attgattaat tcatccgcca
     3901 gttcggcctc aataccggac tgaccttcaa aaacggcggc ctctgttccc ataaccgcat
     3961 ccacagataa accggtatac atcgccactt tttcagcaaa catccggcgc gccgcatcaa
     4021 tgcgctgctg catgtcctgg cgaacctttg ctggcaggga ttcaaactga ttgccatcca
     4081 ccttgtgcga tccggcgtaa atcagcgtga tatccacacc ggcctgtgcc agatgaccgg
     4141 catagctgac atggcccatc atcaccccaa tggagccgat acgggatgtc tgggtaacca
     4201 gccgtcggga gcaggccgat gccagtaaca tggcggctga acaggccgtg tcattgcaca
     4261 gtgcccagac aggcttcagc tgccggagac ggtaaatcat gtcagcgcag tcaaacgcgc
     4321 cggtggcctg cccgccagaa ctgtcaatgt ccagcagtac gccccgtacc tgagtgtccg
     4381 ccattgcctg ctgaagacag gcgacaatgc cgtcatagcc agtcattccg gaaaatggcc
     4441 gcatgccacc cagccggtgt accagcgtgc cggtcaccgg cagtacagca ataccgttca
     4501 ccacccggta aacacgggca ggtcgtttgc ctccggccat gtactcgtcc gtttcagcca
     4561 gcatcccggg agcgtcaagc tgtacctgct gttgcggtac cgaaaggctt gctgcgccca
     4621 tctcacgccc gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggct
     4681 cattgaatgc cgcagcgata atatgtgaaa gattacgtct cacgtgatgt tgtctcctct
     4741 tccggcctgc gactctccgc tatctgctgc tgatacgcct gggctatcca caccggacgt
     4801 gagagtccgg ctttttcgcg ctctgcggat tccctgacct gctggcggaa aatgtcctga
     4861 taatcttcgc ccatcagtgc cagttctttc tcatacgtgc tcagtccggc ctcaatgcgc
     4921 atcaccgatt cctggacctc cttgagcccg tcaatggcca ttcttccgga gctaatccac
     4981 tctgcccgtg accaggctga acgcgcctgg taaaaatcaa aacgcgcccg tggcggacga
     5041 ataatccccc gaagaagcgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg
     5101 gccgcaataa attttcgccg ccccataaaa tagcgccacg actcattggc ggaggcgcgg
     5161 gcacttgagt aactgacctt cgagtaatca cgggacaact gttcgtagga aacgccaaga
     5221 ccggctgcaa tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc
     5281 tgcgctgtct gcaggttcag cgcatcgcca ggaaaaaagg tgcggaattt taacaccgcc
     5341 cagcgtcacg ttattcgtgt cataccagcg ggtgaacttc tccagaatat taataagcgg
     5401 attatcctgc tgaccttgcg gtgcccccgc gatatattca aatgcctttt cggtatcaag
     5461 ttcactttcg attgtggctg catacatcgc cttcacaatg gccgactgaa gctgcgtggc
     5521 ctgcagggag tccagcatct tcatccgctc cattacgctg taaaactgat tagccccacg
     5581 ggtctgcccg tcctccaccg gctcaaaaat atgcagcatg gccggacgcc cggtgggaag
     5641 ctcacgcggg atccgctccc agcgtccggc accagagaac ggaaaatcat cctcacagat
     5701 atggtacgca atcgcccgcc cataccgatc gacctcaacc ccggcccgca gaaagtggtt
     5761 ccccataccg tgtcctggcg tgtccacccg tttcggactc acggctttaa aacgcgtacg
     5821 gaataactgc gtggtttccg tatcccagac cggctgcaca aagatttcgc cgttaaaggc
     5881 atgcacgccc accccttcac ggataaattc cgtaaacgtg cgtttccctt ccacgtcgat
     5941 ctcgccaaac attccttctg cgtattccga ccaggctgct tccacctccc cgacaaaact
     6001 ttttgccgtg gtctcccgca tccccagcca acgccagttc agacggtagc tgataagaaa
     6061 catgtgcccg acgatatgat ccttatgcag ggccaccgca ttagccgcga taccgttatt
     6121 gcgcaccaga tcatctgccc gggcattccc cagacgcaat gcaggcaaca gggccgcatc
     6181 ggcactctgc gccggtggta accactctgc catttgcccg ccaaatcctg cgccgcctcc
     6241 gttgtagctg agactctcac gaagcggaac gccgttcaca tcaatcagga caggcgttcg
     6301 tttcataacc tcactcccag cggtcggcgg cgtcgggtcg tacccagaac cgactccgca
     6361 tcgttgatcg cacggttaag ctcattcagg gacgctgccg tatattcgat cctgcgaccg
     6421 tctttctgta ccgacacaac ccgtttaccg gttaataaat caaggcgcgc ctgacgcagc
     6481 gcctgcagtt cagcgactgt aaccattcac tcctccggac agcttcgctg cc
//
LOCUS       DAZPEU010000194         6520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPEU010000194 DAZPEU010000000
VERSION     DAZPEU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.195"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..115
                     /locus_tag="ACHU2L_003511"
     CDS             <1..115
                     /locus_tag="ACHU2L_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358213.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="ACHU2L_003512"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="ACHU2L_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR1358214.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="ACHU2L_003513"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="ACHU2L_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6520
                     /gene="narH"
                     /locus_tag="ACHU2L_003514"
     CDS             5646..>6520
                     /gene="narH"
                     /locus_tag="ACHU2L_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1358215.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1713 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct
//
LOCUS       DAZPEU010000195         6437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPEU010000195 DAZPEU010000000
VERSION     DAZPEU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.196"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            157..504
                     /locus_tag="ACHU2L_003515"
                     /pseudo
     CDS             157..504
                     /locus_tag="ACHU2L_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            557..952
                     /locus_tag="ACHU2L_003516"
     CDS             557..952
                     /locus_tag="ACHU2L_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR1358216.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="ACHU2L_003517"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="ACHU2L_003517"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR1358217.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHU2L_003518"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHU2L_003518"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR1358218.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHU2L_003519"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHU2L_003519"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR1358219.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="ACHU2L_003520"
     CDS             complement(5323..6423)
                     /locus_tag="ACHU2L_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1358220.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1453 a         1692 c         1540 g         1752 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaat
//
LOCUS       DAZPEU010000196         6401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPEU010000196 DAZPEU010000000
VERSION     DAZPEU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.197"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            21..986
                     /gene="iaaA"
                     /locus_tag="ACHU2L_003521"
     CDS             21..986
                     /gene="iaaA"
                     /locus_tag="ACHU2L_003521"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1358221.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            973..2844
                     /gene="gsiA"
                     /locus_tag="ACHU2L_003522"
     CDS             973..2844
                     /gene="gsiA"
                     /locus_tag="ACHU2L_003522"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR1358222.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            2864..4402
                     /gene="gsiB"
                     /locus_tag="ACHU2L_003523"
     CDS             2864..4402
                     /gene="gsiB"
                     /locus_tag="ACHU2L_003523"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR1358223.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            4420..5340
                     /gene="gsiC"
                     /locus_tag="ACHU2L_003524"
     CDS             4420..5340
                     /gene="gsiC"
                     /locus_tag="ACHU2L_003524"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR1358224.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            5343..6254
                     /gene="gsiD"
                     /locus_tag="ACHU2L_003525"
     CDS             5343..6254
                     /gene="gsiD"
                     /locus_tag="ACHU2L_003525"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR1358225.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         1417 a         1535 c         1873 g         1576 t
ORIGIN      
        1 acccttaatg gacgaatact atgggcaaag cagtcattgc aattcatggt ggcgcaggtg
       61 caattagccg cgcgcagatg agtctgcaac aggaattacg ctacatcgag gcgttgtctg
      121 ccattgttga aaccgggcag aaaatgctgg aagccggcga aagtgcgctg gatgtggtga
      181 cggaagcggt gcgtctgctg gaagagtgtc cactgtttaa cgccggaatt ggcgctgtct
      241 ttacgcgtga tgaaacccat gaactggacg cctgtgtgat ggatggtaac accctgaaag
      301 ccggtgcggt ggcgggcgtt agtcatctgc gtaatccggt tcttgccgcc cggctggtga
      361 tggagcaaag cccgcatgtg atgatgattg gcgaaggggc agaaaatttt gcgtttgctc
      421 atggcatgga gcgcgtctca ccggagattt tctccacgcc tttgcgttat gaacaactac
      481 tggcagcgcg cgaggaaggg gcaacagtcc tcgaccatag cggtgcgcca ctggatgaaa
      541 aacagaaaat gggcaccgtg ggggccgtgg cgttggattt agacggcaat ctggcggcag
      601 ccacgtccac aggcggaatg accaataaat tacccggacg agttggcgat agtcccttag
      661 tgggtgccgg atgctacgcc aataacgcca gtgtggcggt ttcttgtacc ggcacaggcg
      721 aagtcttcat ccgcgcgctg gcggcatatg acatcgccgc gttaatggat tacggcggat
      781 taagtctcgc ggaagcctgc gagcgggtag taatggaaaa actccctgcg cttggcggta
      841 gcggtggctt aatcgctatc gaccatgaag ggaatgtcgc gctaccgttt aacaccgaag
      901 gaatgtatcg cgcctggggc tacgcaggcg atacgccaac caccggtatc taccgtgaaa
      961 aaggggacac cgttgccaca cagtgatgaa cttgatgccg gtaatgtgct ggcggttgaa
     1021 aatcttaata ttgcctttat gcaggaccag cagaaaatag ctgcggtccg caatctctct
     1081 tttagtctgc aacgcggtga gacgctggca attgtaggcg aatccggctc cggtaagtca
     1141 gtgactgcgc tggcattgat gcgtctgttg gaacaggcgg gcggtttagt acagtgcgat
     1201 aaaatgctgt tgcggcggcg cagtcgtgat gtgattgaac ttagcgagca gagcgctgca
     1261 caaatgcgcc atgtgcgcgg tgcggatatg gcgatgatat ttcaggagcc gatgacatcg
     1321 ctgaacccag tatttactgt gggtgaacag attgccgaat caattcgtct gcatcagaac
     1381 gccagtcgtg aagaagcgat ggtcgaggcg aagcggatgc tggatcaggt acgcattccg
     1441 gaggcacaaa ccattctttc acgttattcg catcaactct ctggcgggat gcgccagcga
     1501 gtgatgattg cgatggcgct gtcatgccgc ccggcggtgc tgattgccga tgagccaacc
     1561 accgcgctgg atgtcactat tcaggcgcag atcctgcaat taatcaaagt attgcaaaaa
     1621 gagatgtcga tgggcgttat ctttatcact cacgatatgg gcgtggtggc agagattgcc
     1681 gatcgggtac tggtgatgta tcagggcgag gcggtggaaa cgggtaccgt cgaacagatt
     1741 tttcatgcac cgcaacatcc ttacacccgt gcgctgttag ctgctgttcc gcaacttggt
     1801 gcgatgaaag ggttagatta tccccgacgt ttcccattga tatcgcttga acatccagcg
     1861 aaacaggaac cccccatcga gcagaaaacg gtggtggatg gcgaacctgt tttacgggtg
     1921 cgtaatcttg tcacccgttt ccctttgcgc agcggtttgt tgaatcgcgt aacgcgggaa
     1981 gtgcatgccg ttgagaaagt cagttttgat ctctggcctg gcgaaacgct atcgctggtg
     2041 ggcgagtctg gcagcggtaa atccactacc gggcgggcgt tgctgcgcct ggtcgaatcg
     2101 cagggcggcg aaattatctt taacggtcag cgaatcgata ccttgtcacc tggcaaactt
     2161 caggcattgc gccgggatat tcagtttatt tttcaggacc cttacgcttc gctggaccca
     2221 cgtcagacca tcggtgattc gattatcgaa ccgctgcgtg tacacggttt attgccaggt
     2281 aaagacgcgg ctgcacgcgt tgcgtggttg ctggagcgcg tgggcctgtt acctgaacat
     2341 gcctggcgtt acccgcatga gttttccggc ggtcagcgcc agcgcatctg cattgctcgc
     2401 gcgttggcat tgaatccaaa agtgatcatt gccgacgaag ccgtttcggc gctggatgtt
     2461 tctattcgcg ggcagattat caacttgttg ctcgatctcc agcgtgattt cggcattgcg
     2521 tatctgttta tctcccacga tatggctgtg gtagagcgga ttagtcatcg tgtggcggtg
     2581 atgtatctcg ggcaaattgt tgaaattggt tcacggtgcg cggtcttcga aaacccgcag
     2641 catccttata cgcgtaaatt actggcggca gttccggtcg ctgaaccgtc ccgacaacga
     2701 ccgcagcgtg tactgctgtc ggacgatctt cccagcaata ttcatctgcg tggcgaagag
     2761 gtggcagccg tctcgttgca atgcgtcggg ccggggcatt acgtcgcaca accacaatca
     2821 gaatacgcat tcatgcgtag ataacattca ggcggagaat aaaatggcaa gagctgtaca
     2881 ccgtagtggg ttagtggcgc tgggcattgc gacagcgttg atggcatctt gtgcattcgc
     2941 tgccaaagat gtggtggtgg cggtaggatc gaatttcacc acgctcgatc cgtatgacgc
     3001 gaatgacacg ttatctcagg ccgtagcgaa atcgttttac caggggctgt tcggtctgga
     3061 taaagagatg aaactgaaaa acgtgctggc ggagagttat accgtttccg atgacggcat
     3121 tacttacacc gtgaaattgc gggaaggaat taaattccag gatggcaccg atttcaacgc
     3181 agtggcggta aaagcgaatc tggaccgggc cagcgatccg gcgaatcatc ttaaacgcta
     3241 taacctgtat aagaatattg ccaaaacgga ggcgatcgat ccgacaacgg taaagattac
     3301 cctcaaacag ccgttctcag cgtttattaa tattcttgcc catccggcga ccgcgatgat
     3361 ttcaccgaca gcgctggaaa aatatggcaa ggagattggt tttcatccgg tgggaaccgg
     3421 accgtatgaa ctggatacct ggaatcagac cgattttgtg aaggtgaaaa aattcgcggg
     3481 ttactggcag ccaggattgc ccaaactgga cagcataacc tggcgtccgg tggcggataa
     3541 caacacccgc gcggcaatgc tgcaaaccgg tgaagcgcag tttgctttcc ccattcccta
     3601 cgagcaggcc acactgctgg agaaaaacaa aaatatcgag ttgatggcca gtccgtcaat
     3661 tatgcagcgt tatatcagta tgaacgtgac gcaaaagccg ttcgataacc cgaaggtccg
     3721 tgaggcgctg aattacgcca ttaaccgtcc ggcgctggtg aaagttgcct ttgcgggcta
     3781 tgcaactcca gctactggtg tggtaccgcc aagtatcgcc tacgcgcaaa gttataaacc
     3841 gtggccttac gatccagtga aagcgcgcga attactgaaa gaggcgggat atcccaacgg
     3901 tttcagtacc acgctgtggt cgtcacataa ccacagcacc gcgcagaaag tgctgcaatt
     3961 tacccagcag cagttagcgc aggtcgggat taaagcccag gtgaccgcga tggatgccgg
     4021 acagcgggcg gcagaagttg aaggtaaagg gcaaaaagag agtggcgtgc ggatgttcta
     4081 cactggctgg tcggcttcaa ccggcgaagc cgactgggca ctatcgccgc tgtttgcctc
     4141 gcaaaactgg ccaccgacgc tgtttaatac cgcgttttac agcaataaac aggtggatga
     4201 cttcctggct caggcactga aaacgaatga tccggcggaa aagacccgct tatataaggc
     4261 ggcgcaggat atcatctggc aagaatcgcc gtggatcccg ctggtggtag aaaaactggt
     4321 gtcggcacac agtaaaaacc tgaccggttt ttggatcatg ccagataccg gcttcagctt
     4381 tgaagacgcg gatttgcaat aagcaacgca gggagtggaa tgctttatta cgttatcaaa
     4441 cgattactgg ggttgattcc gacgctgttt atcgtctcgg tgctggtgtt tttatttgtc
     4501 cacatgctgc ccggcgatcc ggcgcgattg attgccgggc ccgaagctga tgcgcaggtt
     4561 atagaactgg tgcgtcagca gctggggttg gatcagccgc tgtatcacca gttctggcac
     4621 tatatcagca atgctgtgca gggggatttt ggcctgtcga tggtgtcgcg tcgtccggtt
     4681 gccgatgaga ttgccagccg ctttatgcca acgctatggc tgaccataac cagtatggtc
     4741 tgggcggtga tatttggtat ggcggcggga attatcgccg ccgtctggcg taatcgttgg
     4801 ccggatcgat tgagtatgac cattgcggtg tcggggatct cgtttccggc atttgcgctg
     4861 gggatgtttt taattcaggt attctccgtt gaactgggct ggctgcctac tgtgggagca
     4921 gacagttggc agcactacat tttaccctcc ctgacgctcg gcgcggcagt ggccgccgtg
     4981 atggcgcgct ttacccgtgc gtcatttgtc gacgttttaa gcgaagatta tatgcgtacc
     5041 gcgagggcga aaggggtgag cgaaacctgg gttgtcctca aacacgggct acgcaacgcg
     5101 atgatcccgg tagtgaccat gatgggctta cagtttggct ttttgctcgg tggttctatc
     5161 gtggtggaga aagttttcaa ctggcccgga cttggacgct tactcgttga ctctgtagaa
     5221 atgcgcgatt acccggtgat tcaggcggaa attctgcttt tctcgctgga atttattctt
     5281 atcaacttag tggtggatgt gctttacgcc gccattaacc cggctatcag gtacaagtaa
     5341 ggatgcgact atttaactgg cgacgtcagg cggtgttaaa cgccatgcca ctggtcaaac
     5401 ctgaccaggt acgtacaccg tggcatgaat tctggcgacg atttcgccgt cagcatatgg
     5461 cgatgaccgc cgcattattc gttattttat tgattgtggt ggccattttt gcacgctgga
     5521 tcgctcccta tgacgccgaa aattattttg attatgacaa tctgaataac ggaccttctt
     5581 tgcagcactg gtttggcgtc gattcactgg ggcgcgacat tttcagccgt gtcctggttg
     5641 gtgcgcaaat ctcgctggcg gcgggcgtgt ttgccgtgtt tatcggtgtg gcgataggga
     5701 cgttgctggg cttgttggct ggatattatg aaggctggtg ggatcggctg atcatgcgca
     5761 tttgcgatgt gctgtttgcc ttcccgggta ttttactggc gatcgctgtt gttgcggtgt
     5821 tgggaagcgg cattgctaac gtgattatcg cagtcgccat tttttccatc cccgcgtttg
     5881 cccgcctggt gcgcggcaac acgctggtgt tgaaacagca aacctttatt gagtcagcac
     5941 gcagtattgg tgccagcgat atgaccattt tattgcgtca tatcctgcct gggaccgtct
     6001 cttctatcgt ggtgtttttc accatgcgca ttggcacctc gattatctct gccgccagcc
     6061 tgtcatttct cggcctcggt gcgcagccgc cgacaccaga gtggggagca atgctcaatg
     6121 aggcacgagc ggatatggtt atcgcgccgc atgtcgctgt ttttccggcc ctggctattt
     6181 ttctgaccgt actggcgttc aatttgttgg gcgatggttt acgcgatgcg ctggatccga
     6241 aaattaaagg atagttacgt ttgaatattg cttgaaaggg taatcacctc acaggaaatt
     6301 attgccctaa gcaagtgttg taactttctg ctgattttgt agaatcgggt aatttggtta
     6361 aaaagccgca gcaagggact atttttgcag cggcacagcg t
//
LOCUS       DAZPEU010000197         6374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPEU010000197 DAZPEU010000000
VERSION     DAZPEU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.198"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..378
                     /locus_tag="ACHU2L_003526"
     CDS             <1..378
                     /locus_tag="ACHU2L_003526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358226.1"
                     /translation="IIRWLLKKRTNGSIRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHU2L_003527"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHU2L_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR1358227.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHU2L_003528"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHU2L_003528"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR1358228.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACHU2L_003529"
     CDS             complement(1787..2476)
                     /locus_tag="ACHU2L_003529"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR1358229.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHU2L_003530"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHU2L_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR1358230.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHU2L_003531"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHU2L_003531"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR1358231.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHU2L_003532"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHU2L_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR1358232.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1621 a         1716 c         1577 g         1460 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagta tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc ttccaactta ctgatttagt gtatgatggt gattttaagg
     6361 tgcttgcgtg gctt
//
LOCUS       DAZPEU010000198         6362 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPEU010000198 DAZPEU010000000
VERSION     DAZPEU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.199"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            186..836
                     /gene="csgD"
                     /locus_tag="ACHU2L_003533"
     CDS             186..836
                     /gene="csgD"
                     /locus_tag="ACHU2L_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR1358233.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            841..1230
                     /gene="csgE"
                     /locus_tag="ACHU2L_003534"
     CDS             841..1230
                     /gene="csgE"
                     /locus_tag="ACHU2L_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR1358234.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1255..1671
                     /gene="csgF"
                     /locus_tag="ACHU2L_003535"
     CDS             1255..1671
                     /gene="csgF"
                     /locus_tag="ACHU2L_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR1358235.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1698..2473
                     /locus_tag="ACHU2L_003536"
                     /pseudo
     CDS             1698..2473
                     /locus_tag="ACHU2L_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2537..3028)
                     /locus_tag="ACHU2L_003537"
     CDS             complement(2537..3028)
                     /locus_tag="ACHU2L_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1358236.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3130..3684)
                     /gene="ycdY"
                     /locus_tag="ACHU2L_003538"
     CDS             complement(3130..3684)
                     /gene="ycdY"
                     /locus_tag="ACHU2L_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR1358237.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3708..4445)
                     /gene="ycdX"
                     /locus_tag="ACHU2L_003539"
     CDS             complement(3708..4445)
                     /gene="ycdX"
                     /locus_tag="ACHU2L_003539"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR1358238.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPCRLLNFLESRGMAPIAEFADL"
     gene            complement(4500..5438)
                     /gene="ghrA"
                     /locus_tag="ACHU2L_003540"
                     /pseudo
     CDS             complement(4500..5438)
                     /gene="ghrA"
                     /locus_tag="ACHU2L_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5673..5760
                     /locus_tag="ACHU2L_003541"
     tRNA            5673..5760
                     /locus_tag="ACHU2L_003541"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5707..5709,aa:Ser,seq:gga)
     gene            complement(5910..6191)
                     /locus_tag="ACHU2L_003542"
                     /pseudo
     CDS             complement(5910..6191)
                     /locus_tag="ACHU2L_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT         1713 a         1552 c         1446 g         1651 t
ORIGIN      
        1 aacacttgat ttaagatttg taatggctag attgaaatca gatgtaatcc attagtttta
       61 tattttaccc atttagggct gatttattac tacacacagc agtgcaacat ctgtcagtac
      121 ttctggtgct tctattttag aggcagctgt caggtgtgcg atcaataaaa aagtggggtt
      181 tcatcatgtt taatgaagtc catagtattc atggtcatac attattgttg atcactaaac
      241 cttctttgca ggcgacagct ctcttgcagc accttaaaca atcgctagca attacaggaa
      301 aattacataa tattcaacgt tctctggacg atatctcttc aggctctatt attcttctgg
      361 atatgatgga agcggataaa aagcttatcc attattggca ggatactttg agcaggaaaa
      421 acaacaatat caaaatattg ttgctaaata cgcctgaaga ttacccgtac cgcgacattg
      481 aaaactggcc tcatatcaac ggcgtttttt atgccatgga ggatcaagaa cgtgttgtca
      541 atgggttgca aggcgtcctg cgtggcgaat gctactttac gcaaaagctt gccagttacc
      601 tgattacaca ttctggtaac tatcgttata acagcacgga atcagccctc cttactcatc
      661 gggaaaaaga gatcctgaat aagctgcgta tcggcgcgtc taataacgag atcgctcgtt
      721 cgttgttcat cagcgaaaat acggttaaaa cgcatcttta taatcttttc aagaagatag
      781 ccgtaaaaaa ccggacacaa gcggtttcct gggcaaacga taacctcagg cgataaagcc
      841 atgaaacgtt atttacgctg gattgtggcg gcagaatttc tgttcgccgc agggaatctt
      901 cacgccgttg aggtagaagt cccgggattg ttaactgacc atactgtttc atctattggc
      961 catgattttt accgagcctt tagtgataaa tgggaaagtg actatacggg taacttaacg
     1021 attaatgaaa ggcccagtgc acgatgggga agctggatca caataacggt caatcaggat
     1081 gttattttcc agactttttt attcccgttg aaaagagact ccgagaaaac tgtcgtcttt
     1141 gcactgattc aaactgaaga agcactaaat cgtcgccaga taaatcaggc gttattaagt
     1201 acggacgatt tggcgcatga tgaattctaa ataaaaaatt gttcggaggc tgcaatgcgt
     1261 gtcaaacatg cagtagttct actcatgctt atttcgccat taagttgggc tggaaccatg
     1321 actttccagt tccgtaatcc aaactttggt ggtaacccaa ataatggcgc ttttttatta
     1381 aatagcgctc aggcccaaaa ctcttataaa gatccgagct ataacgatga ctttggtatt
     1441 gaaacaccct cagcgttaga taactttact caggccatcc agtcacaaat tttaggtggg
     1501 ctactgtcga atattaatac cggtaaaccg ggccgcatgg tgaccaacga ttatattgtt
     1561 gatattgcta accgcgatgg tcaattgcag ttgaacgtga cagatcgtaa aaccggacaa
     1621 acctcgacca tccaggtttc gggtttacaa aataactcaa ccgattttta agccccagct
     1681 tcataaggaa aataatcatg cagcgcttat ttcttttggt tgccgtcatg ttactgagcg
     1741 gatgcttaac cgccccgcct aaagaagccg ccagaccgac attaatgcct cgtgctcaga
     1801 gctacaaaga tttgacccat ctgccagcgc cgacgggtaa gatctttgtt tcggtataca
     1861 acattcagga cgaaaccggg caatttaaac cctacccggc aagtaacttc tccactgctg
     1921 ttccgcaaag cgccagggct tacaaaacct gcttaacgag cgcaaggtta ttcgtgcggc
     1981 acaagaaaac ggcacggttg ccattaataa ccgaatcccg ctgcaatctt taacggcggc
     2041 aaatattatg gttgaaggtt cgattatcgg ttatgaaagc aacgttaaat cgggcggtgt
     2101 tggggcaaga tattttggga tcggtgcaga cacgcaatac cagctcgatc agattgccgt
     2161 gaacctgcgt gtcgtcaatg tgagtaccgg tgagatcctc tcctcggtga acaccagtaa
     2221 gacgatactt tcctatgaag ttcaggccgg ggttttccgc tttattgact accagcgctt
     2281 gctggaaggg gaagtgggtt acacctcaaa cgaacccgtt atgctgtgtc tgatgtcggc
     2341 tatcgaaaca ggggtcattt tcctgattaa tgatggtatc gaccgtggtc tgtgggattt
     2401 gtaaaataaa gcagaacggc agaatgacat tctggtgaaa taccgccata tgtcggttcc
     2461 accggaatcc tgacagataa aaatgccgga attaataatc cggcatttac tgtttaagcg
     2521 aaagcgtgag tgcccctcac gctttatttt tgatttcctg ctcacggtga gccgtctttg
     2581 cactacgcgc cgccagccac aggccgctgt ttttcattgc gtaaccaaat accagcccaa
     2641 gcgccagcga aggtaacacc agtttccagt caccctgccc ggcaaacgtc gcgcatgcgc
     2701 ctataaacgt ccccggaaca aatgaaagca gcagctgttt ggcctgaata cacatcagaa
     2761 acgcgacaat accggttatg acataaccga gaatttccag atgtggtgcc agtgcactac
     2821 cgtaaataat gaccatcgcc cacaccacgc cactaagcag ggttgcagcg gagatcgcca
     2881 gccctttcag cccaccttgc gggcaagcaa agtaggccgt acagcccagg aagcccgccc
     2941 agcttagtaa gcccagggaa acagccaccc atccccagat accggagaga atgcctgttg
     3001 tgattgcaat agagagaagt atgttcatgc cgcgcatgat agcagaaaac gcggccatga
     3061 atgagatcga gcacacattt tatgcaacaa caaattatct gttgcagtta tgagtgacga
     3121 tgatcacatt tactcttcag aatcttcttc cagctcgtcc cacattgcac taatggcatc
     3181 acgggttagc ggtgccatgg tgcgccagaa aggcgtggtt gcatgggcct ccactttgcc
     3241 gaggaacgca ccacaccagg gtaacagata ctcactgaac agtgtttcca gtgcttcgct
     3301 ctcatcttcc gttgactgat cttccagcca ggaagccgca agcagcaatg tgccgatgtg
     3361 atcggctggc gtatccgcca gtagcatccc tcgttcggaa agaaaagcgc gcacttccgc
     3421 ttccgttgcg tcctcaaccc atgcgctacg atatggcggc acggcacatt catcgccgat
     3481 aaacaacgca ttgtaatcgg cagagacttg cgtcatatca cagcttttct gtaaacgcgt
     3541 cagtaactca tcctgctcca gtggccagtt cgcagccagt ttcccctcac gaatcagagt
     3601 aaacagcggc accagtaaag gatcttgcgg ttggcggtaa tagagcgaac ccagcacacg
     3661 acagaggata gaaaactcgt tcattaatat atttccatta ctagtaatta aaggtctgca
     3721 aattccgcaa tcggtgccat accgcgagat tcaaggaagt tcagtaagcg gcacggagaa
     3781 acattcaaaa tgcgctctag cggaaaatct accgcgtcga ggattttaag acactcttca
     3841 aattccccca tggtaaacgc ggtgtgagaa tccgagccta atgccaccca acctccagca
     3901 tcacgtaccg ctgcagctac cgcacgacag ttgtcttcac tgcccttacg tgagtgtaaa
     3961 aatgaggaat tattgatttc cagcgccacc tgatgtttcg cggctgcttc agcaacggct
     4021 ttaacatcta tttcatattt cggatttccg ggatggctta ttatatgcac attgccgctg
     4081 gcgatagtgg agatcatcgc ttgtgtattg gtcgctttgt catgtggcgc aaaaaccggc
     4141 tcatgaaaac cggcaataat cagatccagc gagtcaaaca ttttaccgct gcagtcaatt
     4201 tcaccatcaa catttttaat gttagcttca atgccgcgca ggatccctac cccatcaacc
     4261 actcgcggcc agatacgcat gttaatgaag tgccagtgat gcggcgcatc ttccatatcc
     4321 gggccatgat cggtgatcgc aaaaagttta atgccctttt gtttggcctg ggcaatgtaa
     4381 tcactcaatg tgctatatgc atgtgtgctg gcaacggtat gcatatgaag gtcgacggga
     4441 tacataactc tctcctgtat tcaattcccg ccaaggatag caggaatcct gatgctttat
     4501 tagtagccac gtgcgcggtc gacttgcccg cagaccctct cccctttttc gagctgggca
     4561 atagtgcgag aaatgtactc cacagcttca gcgggacggg taatcgcggc gacatgtggt
     4621 gttatcgtca cgcgtggatg ttgccagagc ggactttcag gcggtaaggg ttcacgatta
     4681 aaaacatcca gcattgcgcc tttaactttc ccgctatcca gcgccgcgag caggtcatct
     4741 tccacaacat gaacaccacg cgccaggttg aggagatacg cgccatcagg taatttttcg
     4801 agtaattgtt gattaataat gccgacggtt tcaggggtat tcggtaacaa attaatcaat
     4861 acccgacatt ggctcagaaa tgcagacagt tcttcccgtc cggcaaagct ttgcacgcca
     4921 ggccacgatt tacgggttcg actccagcaa cgcagcggaa agcgctaggt ttgcagactc
     4981 tgagcaactt tacagcccag tacgcctgcg cccaaaatgc cgatggtaaa atcttcccga
     5041 tgatattcag gcagcggttg ccaatgcgaa ctattttgct ggatgcgata atcgtcaaaa
     5101 cgtcgaaacc aatgcagcac ctgactgaca gcatattcct gcatttgctc gcccataccg
     5161 gtatcttcca ggcgaaaaag tggaacagaa gggttcagca tttcagggtg tgcctgtagc
     5221 ttgctcaaaa tagaatcaac accggccccg agtgcgaaca ccgctttaag atcgcgccct
     5281 gccagcattt caacaggagg atgccagact aaaacataat cagcagagtc attatctccg
     5341 cttttccatg ccctgactct tgcctgagga atagctttgc gcagtgcctc aatccaccat
     5401 tgggtatcga acgttgggtg ataaaagatg atatccatac tgactcccga aaagcgttgt
     5461 gcgaaattta ttcgcactta tcgttatgat ctacaaaggc caccagcata acaaatccgt
     5521 ggtcggtggc aaaaaaagca gatttcgctt attaaaacca cacattgatt gaatgtttga
     5581 ataaacgcgc gattttttca aaaagtttgt tgacctcagg tcatgatttc cctaaattag
     5641 cgcccgttcc agcaagacag gaacgacaat ttggtgaggt gtccgagtgg ctgaaggagc
     5701 acgcctggaa agtgtgtata cggcaacgta tcgggggttc gaatcccccc ctcaccgcca
     5761 gattataagt gcatgttatt gtttaacatg cgctttttta tttttccttc cagacagtat
     5821 gccatcgggt atgccatctg aaaccggttt atatgccccg cgatcatatc ttatcggaca
     5881 atttccgatc tttgagcata ctggcaaagc tatactgcca gcatatcagg atagtgtttt
     5941 gcgataatat cattcagtct gtcaaccagt tctggttttc tgaacgtgat atgcgcggag
     6001 cccttcttaa agtatctgat gctgaacatc tcatcttcat agctctcttt accctgaaca
     6061 gactgaatgt gatcatccag acgaagggtg atgtttcccc ggttgtcggg gattggttta
     6121 ccgctgaaca ggtgcaacat tctttccaga tcggcgagtc ggtcagcctg ttgtccggtg
     6181 ataagatgaa atctatggtc acccccgttt ttgcaacact gatttttgat taatggttgg
     6241 cttgcttaaa tctatccggc gtctctgtgg gcaaggatgc ccgcgccccg atgagttccg
     6301 cacctgataa tcctaaaaat cccctcggct tcagtgagcc atttcatctg ttaggttttt
     6361 aa
//
LOCUS       DAZPEU010000199         6344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPEU010000199 DAZPEU010000000
VERSION     DAZPEU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.200"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            127..2570
                     /gene="dmsA"
                     /locus_tag="ACHU2L_003543"
                     /pseudo
     CDS             127..2570
                     /gene="dmsA"
                     /locus_tag="ACHU2L_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            2581..3198
                     /gene="dmsB"
                     /locus_tag="ACHU2L_003544"
     CDS             2581..3198
                     /gene="dmsB"
                     /locus_tag="ACHU2L_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1358239.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            3200..3991
                     /locus_tag="ACHU2L_003545"
     CDS             3200..3991
                     /locus_tag="ACHU2L_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR1358240.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(4027..4653)
                     /gene="ycaC"
                     /locus_tag="ACHU2L_003546"
     CDS             complement(4027..4653)
                     /gene="ycaC"
                     /locus_tag="ACHU2L_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR1358241.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            4968..6116
                     /locus_tag="ACHU2L_003547"
     CDS             4968..6116
                     /locus_tag="ACHU2L_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1358242.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            6134..>6344
                     /locus_tag="ACHU2L_003548"
     CDS             6134..>6344
                     /locus_tag="ACHU2L_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1358243.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         1486 a         1508 c         1691 g         1659 t
ORIGIN      
        1 ctacctatct ctttgatttt caaattattc tatgtataca agcctatata gcgagctgct
       61 atagaaataa ttacacaata cggtttgtta ctggaatcaa tcgtgatcaa gcttgagtga
      121 gccattatga aaacgaaaat ccctgatgcg gtattggctg ctgaggagag tcgccgtggt
      181 ttggtaaaaa cgacagcgat cggcggcctg gcaatggcca gcagcgcatt aacattacct
      241 tttagtcgga ttgcgcacgc tgtcgatagc gccattccaa caaaatcaga cgaaaaggtt
      301 atctggagcg cctgtacagt taactgtggt agtcgctgcc cgctacgtat gcacgtcgtg
      361 gacggtgaaa tcaaatatgt cgaaacgaca ataccggcga tgacaattac gacggcctac
      421 actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtca ccgtgtctac aatccggacc
      481 gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga aggcaaattc gagcgcatta
      541 gctgggaaga agcctacgac atcatcgcga ccaatatgca gcgcctgatc aaagagtacg
      601 gcaacgagtc tatctatctg aactatggca ccggtacgct gggcggcacc atgacccgct
      661 cctggccgcc gggaaatacc ctggtcgcgc gcctgatgaa ctgctgcggc ggttatctta
      721 accattacgg tgactactct tccgcgcaaa ttgcggaagg tttgaaccat acctacggcg
      781 gctgggcaga tggtaacagc ccgtcggata tcgaaaacag taaattggta gtgctgtttg
      841 gtaataaccc aggcgaaacg cgaatgagtg gcggtggggt gacttactat cttgaacagg
      901 cacgccagaa atctaatgct cgcatgatca tcatcgatcc gcgctatacc gacaccggtg
      961 ccgggcgcga agatgagtgg atccctattc gtccaggaac agatgccgca ctggttaacg
     1021 gtctggcgta cgtcatgatc actaaaaacc tggtggatca ggcattcctc gataaatatt
     1081 gcgttggcta tgacgagaaa accctgcccg ccagtgcgcc gaaaaatggt cattataaag
     1141 cttatattct gggtgaaggg ccagatggcg tggctaaaac accggaatgg gcctcgcaaa
     1201 tcaccggtgt tccggcagac aaaatcatca aactggctcg tgaaatcggc agtaccaaac
     1261 cggtgtttat cagccaggga tggggcccgc agcgtcacgc taacggtgaa atcgcaaccc
     1321 gtgctatctc gatgctggcg attctgaccg gtaacgttgg tattaatgga gggaacagcg
     1381 gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat gccgaccttg gaaaacccga
     1441 tccagaccag catttcgatg tttatgtgga ccgatgccat tgaacgtggc ccggaaatga
     1501 cggcgctgcg tgatggtgtg cgcgggaaag ataagctgga tgtgccgatc aaaatgatct
     1561 ggaactatgc cggtaactgc ctgattaacc agcattctga aatcaaccgt acccatgaaa
     1621 tcctccagga tgataagaag tgcgagctga ttgtggttat cgactgccac atgacctcat
     1681 cggcgaaata tgctgacatc ctgctgcctg actgcaccgc ttccgaacag atggactttg
     1741 cactggatgc atcctgcggg aatatgtctt acgtgatttt caacgatcag gtgattaaac
     1801 cgcgctttga atgtaagacc atctatgaaa tgaccagcga actggcaaaa cgtcttggcg
     1861 ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg gatgcggcat ctgtatgccc
     1921 agtcgcggga agcgattcct gaactgccaa cgtttgaaga gttccgcaag caggggatct
     1981 ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa agccttccgt gaagatccgc
     2041 aggcaaaccc actgactacg ccatcgggca aaattgagat ttattcgcag gcgctggctg
     2101 acattgccgc tacctgggaa ttgccagaag gcgatgtgat cgatccactg ccgatctaca
     2161 cgccgggctt tgaaagttat caggatccgc tgaacaaaca gtatccgctg cagcttacag
     2221 gtttccacta taaatctcgc gttcactcaa cttacggcaa cgttgatgtg ctgaaagcgg
     2281 cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca aaaacgcggt atccacaacg
     2341 gcgataaagt caggatcttt aacgatcgtg gtgaggttca tattgaggcg aaagtgacgc
     2401 cacgaatgat gccgggcgtg gtcgcactgg gtgaaggcgc ctggtatgac ccggatgcaa
     2461 aacgtgtcga taagggcggt tgtatcaacg tactgaccac tcaacgtccg tctcctctcg
     2521 ctaaggggaa tccgtcacat acaaaccttg ttcaggttga aaaggtgtaa ggagtaaccg
     2581 atgacaaccc agtatggatt ttttattgat tccagccgtt gcaccggttg caaaacctgc
     2641 gagctggctt gtaaagacta caaagatttg acgccagaag tcagtttccg ccgcatttat
     2701 gaatatgctg gcggcgactg gcaggaagat aacggtgtct ggcaccagaa cgtgtttgcc
     2761 tactatctgt cgatttcgtg caaccactgc gaagatccgg cttgtactaa agtctgcccg
     2821 agcggtgcga tgcataaacg cgaagatggt tttgttgtgg tcgatgaaga tgtatgcatt
     2881 ggctgccgtt actgtcatat ggcttgcccg tatggcgcac cgcaatataa cgaaacgaaa
     2941 ggccatatga ccaaatgcga tggttgttat gaccgtgttg cggagggtaa aaagccgatc
     3001 tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc ctatcgacga gctgcgtaaa
     3061 aaacatggcg atctggcggc cgttgcgccg ttgccgcgag ctcactttac caaaccgaat
     3121 attgtgatca aacccaatgc caatagccgc ccgaccgggg ataccactgg ctatctggca
     3181 aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa tggccgctga tgatcttcac
     3241 ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg gctttggcgc tgctcaaagg
     3301 cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc atgtttggct tatgggtact
     3361 gatgggcatt ggctttatcg cctctatgct ccatcttggt tcaccgatgc gcgcctttaa
     3421 ctcgctcaac cgggtagggg cttcagcact cagtaacgaa atcgccagcg gttcgatctt
     3481 ttttgctgta ggcggcatcg gctggttgct ggcaatgctg aaaaagctgt caccggcatt
     3541 gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc gtctttgtct ggatgatggt
     3601 gcgtgtgtat aacagcattg ataccgttcc gacctggtac agcatctgga cgccgatggg
     3661 ctgggcgatg cgtctgctgc cagccatttc tgtactggca ctggtggtga gtggcgtgat
     3721 gtcagtgatg cagggcacag agctggcaac tattcatagt tctgtgcagc aggccgcagc
     3781 gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct
     3841 gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc tactatccgt
     3901 ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct atggtttgca
     3961 tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt cggccccggt
     4021 tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac gataatcagg
     4081 aatatggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt cacggtgcag
     4141 ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacca gcttgcgaca tgcgatccca
     4201 cgccgaatgc cgggtaattt cattaaaggt ccctgatgca tcagtcacga caaagacatc
     4261 aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg ttaccacacc
     4321 ggcaataatt aactgttttt tacctgtcgc tttgactgct tttacaaaat cttcgttatc
     4381 ccaggcgtta atattgcccg ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag
     4441 ttctagaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa tagttggtaa
     4501 gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact tatcgggttc
     4561 gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa cggcagcatc
     4621 atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag agtcagtggt
     4681 acgtctgaaa tcggtcagac gcgatgcatt gctctgaaag catagacggg aaatatgagt
     4741 ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat
     4801 aaaaacaatc agatgagatt aatcatctct tcagcattag cgttatgaat gagtaatata
     4861 aatcgataat accgccgctg cgatatgcgc gtattttcac ccattgacaa tgtttttggc
     4921 ggtggcatga tgcgcatgaa atttgaactt cctcacggtt ttaattcatg tccacgtata
     4981 cccggcctgt catgcttttg ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt
     5041 taaatacact cgtgccgctt tggctcgccc aggaacacat gtccacatgg caggtaggcg
     5101 ttgtcagctc atcctatttt accggcaacc ttgtcggtac attgctgaca gggtatgtca
     5161 ttaaacgcat tggctttaac cgcagctatt atctgtcctc cttcattttt gctgctggct
     5221 gtgccggcct tggcctgatg attggtttct ggagctggtt ggcgtggcgt tttgtcgcgg
     5281 gcgtcggctg tgccatgatt tgggtggttg ttgagagcgc gctgatgtgc agtgggacgt
     5341 cacgtaaccg tgggcgtttg cttgctgcgt atatgatggt ttattacgtg ggaacgtttt
     5401 taggccagtt actggtcagc aaagtctcaa ccgagctgat gagcgtattg ccgtgggtta
     5461 caggtttgac attggcaggg atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg
     5521 ctgaaaacca tgattcgacg tcaattacgg caatgctaaa actccgtcag gcgcggcttg
     5581 gcgtgaatgg ctgcattatc tcaggaatcg ttctgggatc tctatatggc ctgatgcctc
     5641 tgtacctcaa tcacaaaggg gtgagcaatg ccagtattgg tttctggatg gcggtactgg
     5701 tcagtgcggg tatcctcgga caatggccga ttggacgtct ggcggataag tttggtcgac
     5761 tgctggtttt gcgtgttcag gtctttgtcg tcattctcgg cagtattgcg atgcttagcc
     5821 aggcggcgat ggccccagcg ttattcatcc tcggtgccgc tggctttacg ctatatccgg
     5881 tggcgatggc ctgggcttgt gagaaagttg aacatcatca actggtggcg atgaaccaag
     5941 ccttactgtt gagctatacc gtgggaagtc tgcttggccc gtcatttagc gctatgctaa
     6001 tgcagaattt ctccgataat ttattgttta tcatgatcgc cagcgtatcg tttatctatt
     6061 tgctgatgct gcttcgcaac gccggtcata cgccgaaacc cgttgctcac gtgtaaatga
     6121 attcaagcaa agtgtgaact tactgtttca cactctgctt ttttgtttct tctatctgac
     6181 ttgctttttt ccaaatttta ttcgtttaaa aataaaatgt gcagcaggtt ataattttgc
     6241 atttcgctat ttccgcactt cttatttgcc gcgcataatc cctcgtttta cagatgcccc
     6301 tttaattttg gcgaaggatt tgtctatggc tgggaatgtt cagg
//
LOCUS       DAZPEU010000200         6257 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPEU010000200 DAZPEU010000000
VERSION     DAZPEU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.201"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHU2L_003549"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHU2L_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1358244.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHU2L_003550"
     CDS             2219..2767
                     /locus_tag="ACHU2L_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR1358245.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHU2L_003551"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHU2L_003551"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR1358246.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHU2L_003552"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHU2L_003552"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR1358247.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHU2L_003553"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHU2L_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR1358248.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1566 a         1540 c         1594 g         1557 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaa
//
LOCUS       DAZPEU010000201         6237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPEU010000201 DAZPEU010000000
VERSION     DAZPEU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.202"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..856)
                     /locus_tag="ACHU2L_003554"
     CDS             complement(<1..856)
                     /locus_tag="ACHU2L_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1358249.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNV"
     gene            964..1242
                     /locus_tag="ACHU2L_003555"
     CDS             964..1242
                     /locus_tag="ACHU2L_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358250.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHU2L_003556"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHU2L_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1358251.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHU2L_003557"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHU2L_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR1358252.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHU2L_003558"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHU2L_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR1358253.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHU2L_003559"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHU2L_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR1358254.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="ACHU2L_003560"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="ACHU2L_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="ACHU2L_003561"
     CDS             complement(4922..5452)
                     /locus_tag="ACHU2L_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1358255.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHU2L_003562"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHU2L_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR1358256.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1816 a         1305 c         1350 g         1766 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctga
//
LOCUS       DAZPEU010000202         6232 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPEU010000202 DAZPEU010000000
VERSION     DAZPEU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.203"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..455
                     /gene="ipgC"
                     /locus_tag="ACHU2L_003563"
     CDS             <1..455
                     /gene="ipgC"
                     /locus_tag="ACHU2L_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR1358257.1"
                     /translation="TENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAYDFYNK
                     GRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKNDYTPV
                     FHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            461..2203
                     /gene="ipaB"
                     /locus_tag="ACHU2L_003564"
     CDS             461..2203
                     /gene="ipaB"
                     /locus_tag="ACHU2L_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR1358258.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            2166..3314
                     /gene="ipaC"
                     /locus_tag="ACHU2L_003565"
     CDS             2166..3314
                     /gene="ipaC"
                     /locus_tag="ACHU2L_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR1358259.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            3365..4363
                     /gene="ipaD"
                     /locus_tag="ACHU2L_003566"
     CDS             3365..4363
                     /gene="ipaD"
                     /locus_tag="ACHU2L_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR1358260.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            4372..>6232
                     /gene="ipaA"
                     /locus_tag="ACHU2L_003567"
     CDS             4372..>6232
                     /gene="ipaA"
                     /locus_tag="ACHU2L_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR1358261.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVT"
BASE COUNT         2321 a         1260 c         1036 g         1615 t
ORIGIN      
        1 tcaccgaaaa tgaaagcatc tctactgcag taattgatgc aattaactct ggcgctacac
       61 tgaaagatat taatgcaatt cctgatgata tgatggatga catttattca tatgcttatg
      121 acttttacaa caaaggaaga atagaggaag ctgaagtttt cttcaggttt ttatgtatat
      181 acgactttta caatgtagac tacattatgg gactcgcagc tatttatcag ataaaagaac
      241 agttccaaca agcagcagac ctttatgctg tcgcttttgc attaggaaaa aatgactata
      301 caccagtatt ccatactgga caatgccagc ttcggttgaa agccccctta aaagctaaag
      361 agtgcttcga actcgtaatt caacacagca atgatgaaaa attaaaaata aaagcacaat
      421 catacttgga cgcaattcag gatatcaagg agtaattatt atgcataatg taagcaccac
      481 aaccactggt tttcctcttg ccaaaatatt ggcttccact gagcttggag acaatactat
      541 ccaagctgca aatgatgcag ctaacaaatt attttctctt acaattgctg atcttactgc
      601 taaccaaaat attaatacaa ctaatgcaca ctcaacttca aatatattaa tccctgaact
      661 taaagcacca aagtcattaa atgcaagttc ccaactaacg cttttaattg gaaaccttat
      721 tcaaatactc ggtgaaaaat ctttaactgc attaacaaat aaaattactg cttggaagtc
      781 ccagcaacag gcaagacagc aaaaaaacct agaattctcc gataaaatta acactcttct
      841 atctgaaact gaaggactaa ccagagacta tgaaaaacaa attaataaac taaaaaacgc
      901 agattctaaa ataaaagacc tagaaaataa aattaaccaa attcaaacaa gattatccga
      961 actcgaccca gagtcaccag aaaagaaaaa attaagccgg gaagaaatac aactcactat
     1021 caaaaaagac gcagcagtta aagacaggac attgattgag cagaaaaccc tgtcaattca
     1081 tagcaaactt acagataaat caatgcaact cgaaaaagaa atagactctt tttctgcatt
     1141 ttcaaacaca gcatctgctg aacagctatc aacccagcag aaatcattaa ccggacttgc
     1201 cagtgttact caattgatgg caacctttat tcaactagtt ggaaaaaata atgaagaatc
     1261 tttaaaaaat gatctggctc tattccagtc tctccaagaa tcaagaaaaa ctgaaatgga
     1321 gagaaaatct gatgagtatg ctgctgaagt acgtaaagca gaagaactca acagagtaat
     1381 gggttgtgtt gggaaaatac ttggggcact tttaactatc gttagtgttg ttgcagcagc
     1441 tttttctgga ggagcctctc tagcactggc agctgttggt ttagctctta tggttacgga
     1501 tgctatagta caagcagcga ccggcaattc cttcatggaa caagccctga atccgatcat
     1561 gaaagcagtc attgaaccct taatcaaact cctttcagat gcatttacaa aaatgctcga
     1621 aggcttgggc gtcgactcga aaaaagccaa aatgattggc tctattctgg gggcaatcgc
     1681 aggcgctctt gtcctagttg cagcagtcgt tctcgtagcc actgttggta aacaggcagc
     1741 agcaaaactt gcagaaaata ttggcaaaat aataggtaaa accctcacag accttatacc
     1801 aaagtttctc aagaattttt cttctcaact ggacgattta atcactaatg ctgttgccag
     1861 attaaataaa tttcttggtg cagcgggtga tgaagtaata tccaaacaaa ttatttccac
     1921 ccatttaaac caagcagttt tattaggaga aagtgttaac tctgccacac aagcgggagg
     1981 aagtgtcgct tctgctgttt tccagaacag cgcgtcgaca aatctagcag acctgacatt
     2041 atcgaaatat caagttgaac aactgtcaaa atatatcagt gaagcaatag aaaaattcgg
     2101 ccaattgcag gaagtaattg cagatctatt agcctcaatg tccaactctc aggctaatag
     2161 aactgatgtt gcaaaagcaa ttttgcaaca aactactgct tgatacaaat aaggagaatg
     2221 ttatggaaat tcaaaacaca aaaccaaccc agattttata tacagatata tccacaaaac
     2281 aaactcaaag ttcttccgaa acacaaaaat cacaaaatta tcagcagatt gcagcgcata
     2341 ttccacttaa tgtcggtaaa aatcccgtat taacaaccac attaaatgat gatcaacttt
     2401 taaagttatc agagcaggtt cagcatgatt cagaaatcat tgctcgcctt actgacaaaa
     2461 agatgaaaga tctttcagag atgagtcaca cccttactcc agagaacact ctggatattt
     2521 ccagtctttc ttctaatgct gtttctttaa ttattagtgt agccgttcta ctttctgctc
     2581 tccgcactgc agaaactaaa ttgggctctc aattgtcatt gattgcgttc gatgctacaa
     2641 aatcagctgc agagaacatt gttcggcaag gcctggcagc cctatcatca agcattactg
     2701 gagcagtcac acaagtaggt ataacgggta tcggtgccaa aaaaacgcat tcagggatta
     2761 gcgaccaaaa aggagcctta agaaagaacc ttgccactgc tcaatctctt gaaaaagagc
     2821 ttgcaggttc taaattaggg ttaaataaac aaatagatac aaatatcacc tcaccacaaa
     2881 ctaactctag cacaaaattt ttaggtaaaa ataaactggc gccagataat atatccctgt
     2941 caactgaaca taaaacttct cttagttctc ccgatatttc tttgcaggat aaaattgaca
     3001 cccagagaag aacttacgag ctcaataccc tttctgcgca gcaaaaacaa aacattggcc
     3061 gtgcaacaat ggaaacatca gccgttgctg gtaatatatc cacatcagga gggcgttatg
     3121 catctgctct tgaagaagaa gaacaactaa tcagtcaggc cagcagtaaa caagcagagg
     3181 aagcatccca agtatctaaa gaagcatccc aagcgacaaa tcaattaata caaaaattat
     3241 tgaatataat tgacagcatc aaccaatcaa agaattcgac agccagtcag attgctggta
     3301 acattcgagc ttaatatatc caagagccat aataatatat ggctcttcct gtaaggaaat
     3361 aaccatgaat ataacaactc tgactaatag tatttccacc tcatcattca gtccaaacaa
     3421 taccaacggt tcatcaaccg aaacagttaa ttctgatata aaaacaacga ccagttctca
     3481 tcctgtaagt tcccttacta tgctcaacga cacccttcat aatatcagaa caacaaatca
     3541 ggcattaaag aaagagcttt cacaaaaaac gttgactaaa acatcgctag aagaaatagc
     3601 attacattca tctcagatta gcatggatgt aaataaatcc gctcaactat tagatattct
     3661 ttccaggcac gaatatccaa ttaataaaga cgcaagagaa ttattacatt cagctccgaa
     3721 agaagccgag cttgatggag atcaaatgat atctcataga gaactgtggg ctaaaattgc
     3781 aaactccatc aatgatatta atgaacagta tctgaaagta tatgaacatg ccgttagttc
     3841 atatactcaa atgtatcaag attttagcgc tgttctttcc agtcttgccg gctggatctc
     3901 tcccggaggt aacgacggaa actccgtgaa attacaagtc aactcgctta aaaaggcatt
     3961 ggaagaactc aaggaaaaat ataaagataa accgctatat ccagcaaata atactgttag
     4021 tcaggaacaa gcaaataaat ggcttacaga attaggtgga acaatcggca aggtatctca
     4081 aaaaaacggg ggatatgttg tcagtataaa catgacccca atagacaata tgttaaaaag
     4141 cttagataat ctaggtggaa atggcgaggt tgtgctagat aatgcaaaat atcaggcatg
     4201 gaatgccgga ttctctgccg aagatgaaac aatgaaaaat aatcttcaaa ctttagttca
     4261 aaaatacagt aatgccaata gtatttttga taatttagta aaggttttga gtagtacaat
     4321 aagctcatgt acagatacag ataaactttt tctccatttc tgaggtgcgc catgcataat
     4381 gtaaataata ctcaagcgcc aacattctta tataaggcaa cttcaccatc atcaacagaa
     4441 tacagcgagt taaaaagcaa aatatccgat atccatagtt cgcaaacttc tctaaaaaca
     4501 ccagcatcag tgtctgaaaa agaaaacttt gcaacgtctt ttaatcagaa atgtcttgat
     4561 tttttatttt cttcctcagg gaaagaagat gtgttaagaa gcatttattc caactcaatg
     4621 aatgcgtatg ccaaaagcga gattctcgaa ttttcaaatg ttttgtactc cttagtacat
     4681 caaaatggtc ttaattttga aaacgaaaag ggacttcaaa aaattgtcgc acagtattcg
     4741 gaactaatta taaaagataa attatcccaa gattctgcct ttggaccatg gtcggcaaag
     4801 aataagaaac tccatcaatt acgacaaaac attgagcaca gacttgcact attagcacaa
     4861 caacacacat ctggtgaagc tttatcattg ggacaaaaac tcctcaatac tgaagtatca
     4921 tcatttatca agaataatat tcttgctgaa ttaaagttaa gtaatgaaac tgtttcatct
     4981 ctcaaactag atgatttagt tgacgcacag gcaaaacttg cctttgatag tttgcgcaat
     5041 caacgtaaaa atactattga tagtaaagga tttggtatag gtaaactgtc aagagactta
     5101 aatacagtag ccgtgtttcc tgagctgttg agaaaagtcc ttaatgatat tttagaagat
     5161 ataaaagatt cgcatcctat ccaagatggc ctccctacac ctcccgaaga tatgccagat
     5221 ggcggaccaa cccccggagc caatgagaaa acatcccaac ctgtaattca ctatcatata
     5281 aataatgata atagaactta cgataataga gtttttgaca acagagtata tgacaatagc
     5341 tatcacgaga acccagaaaa tgatgcacag tctcctactt ctcagacaaa cgatctatta
     5401 tcccgtaacg gaaactcatt actaaatcca caaagagcac tagttcaaaa agtaacttcc
     5461 gttctaccac actctatatc agatactgtc cagacatttg caaataattc agctttagaa
     5521 aaggctttca accatactcc agataattcg gatggaatag gttcagacct gttaactacg
     5581 agtagtcaag aaagatctgc aaataactct ctttctcggg gacacaggcc tctgaacata
     5641 cagaactctt caaccacccc ccctctccac ccggaaggag tgacaagcag taatgataac
     5701 tcatcagata caactaaaag tagcgcttct ctttctcata gagtagcttc gcaaatcaat
     5761 aaattcaact caaacactga ttcaaaagta cttcagactg attttttatc aagaaatgga
     5821 gacacatatt taacacggga aacgatattt gaagcttcaa aaaaagtaac aaactcccta
     5881 agtaatctta tatctctcat tggaactaaa tcaggaacac aagaacgaga gttacaggaa
     5941 aaatcaaagg acattacaaa atccacaaca gaacatagaa taaacaacaa attaaaagtt
     6001 acagatgcaa atataagaaa ctacgtaaca gaaaccaacg cagatacaat tgataaaaat
     6061 catgcgatct atgaaaaggc aaaagaagta tctagcgccc tcagcaaggt attgtcaaaa
     6121 attgacgata cctctgcaga attacttaca gatgatatat ctgatttaaa aaataacaat
     6181 gatattacag ctgaaaacaa taatatatat aaagcagcaa aagatgtaac ca
//
LOCUS       DAZPEU010000203         6110 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPEU010000203 DAZPEU010000000
VERSION     DAZPEU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.205"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..384)
                     /gene="yneK"
                     /locus_tag="ACHU2L_003568"
     CDS             complement(<1..384)
                     /gene="yneK"
                     /locus_tag="ACHU2L_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR1358262.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFC"
     gene            458..1399
                     /locus_tag="ACHU2L_003569"
                     /pseudo
     CDS             458..1399
                     /locus_tag="ACHU2L_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            1463..2389
                     /gene="glsB"
                     /locus_tag="ACHU2L_003570"
     CDS             1463..2389
                     /gene="glsB"
                     /locus_tag="ACHU2L_003570"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR1358263.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            2389..2733
                     /locus_tag="ACHU2L_003571"
     CDS             2389..2733
                     /locus_tag="ACHU2L_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR1358264.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            2887..4305
                     /locus_tag="ACHU2L_003572"
     CDS             2887..4305
                     /locus_tag="ACHU2L_003572"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1358265.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            4532..5983
                     /gene="uxaB"
                     /locus_tag="ACHU2L_003573"
     CDS             4532..5983
                     /gene="uxaB"
                     /locus_tag="ACHU2L_003573"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR1358266.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1451 a         1379 c         1621 g         1659 t
ORIGIN      
        1 gcaaaaaata ttaatttctc catcatggcg aaaattatct aaataaatag ggcttaaatt
       61 tattactacc tgaaattcaa tattttcttt gtgtcttcca ttcttgacag gataagtgag
      121 tttaaactga accttttcat tgtatagtgt gtcgttaccc cgaaaagaag tcctctgaat
      181 atatgataag ttatcaaggt tgttaaatat cttttttgtg gctgagttgg tgtttaaaga
      241 cgtgcgccac tcttcatcac atattatggt actgagtgca tcaataaaat cctttaaaac
      301 ttcttttact tgaggtgcaa tattacgagc gggaaaatca tcaggaagag atatgtaatt
      361 gcagatgctt actggcgtaa ccatatttgc cccatatagt cataagtcat cggaacatca
      421 ctatataggg catttgagct gcaagaacta tcaaaggcag gtgatgcgtg gcgctgttcc
      481 catcattctt gcaggtaacg gctacttact taaacatgcg ccgaatgtga tgggctgtgc
      541 tcagctcatt gcccaagtgt ttaaagatgc gggaatcccg caaggcgtat atggctggct
      601 gaatgccgac aacgacggtg tcagtcagat gattaaagac tcgcgcattg ctgctgtcac
      661 ggtgaccgga agtgttcgtg ctggagcggc actgaaaaaa tgcgtactgg aactgggcgg
      721 ttcggatcca tttattgtgc ttaacgatgc cgatctggaa ctggcggtta aagcggcggt
      781 agccggacgt tatcagaata ccggacaggt ttgtgcagcg gcaaaacgct ttattatcga
      841 agagggaatt gcttcggcat ttaccgaacg ttttgtgtca gctgcggcag ccttgaaaat
      901 gggcgatccc cgtgatgaag agaacgctct cggaccaatg gctcgttttg atttacgtga
      961 tgagctgcat catcaggtgg agaaaaccct ggcgcagggt gcgcgtttgt tactgggcgg
     1021 ggaaaagatg gctggagcag gtaactacta tccgccaacg gttctggcga atgttacccc
     1081 agaaatgacc gcgtttcggg aagaaatgtt tggccccgtt gctgcaatca ccattgcgaa
     1141 agatgcagaa catgcgctgg aactggctaa tgatagtgag ttcggccttt cagcgaccat
     1201 ttttaccacc gacgaaacac aggccagaca gatggcggca cgtctggaat gcggtggggt
     1261 gtttatcaat ggttattgtg ccagcgacgc gcgagtggcc tttggtggcg tgaaaaagag
     1321 tggctttggt cgtgagcttt cccatttcgg cttacacgaa ttctgtaata tccagacagt
     1381 gtggaaagac cggatctgac gcgacatatt cagctctgat atactcgcag gtcttttcag
     1441 acctgcggtc caggagtaga aagtggcagt cgccatggat aatgcaattt tagaaaacat
     1501 cttgcggcaa gtgcggccgc tcattggtca gggtaaagtc gcggattata ttccggcgct
     1561 ggctacagta gacggttccc gattggggat tgctatctgt accgttgacg gacagctttt
     1621 tcaggccgga gacgcgcaag aacgtttttc cattcagtct atttccaaag tgctgagtct
     1681 cgttgtcgcc atgcgtcatt actccgaaga ggaaatctgg caacgcgtcg gcaaagatcc
     1741 gtctggatca ccgttcaatt ctttagtgca actggaaatg gaacagggta ttccgcgtaa
     1801 tccgttcatt aataccggtg cgctggtggt ctgcgatatg ttgcaagggc gactaagcgc
     1861 accacggcaa cgtatgctgg aagtcgtgcg cggcttaagc ggtgtgtctg atatttcata
     1921 cgatacggtg gtagcgcgtt ccgaatttga acattccgcg cgaaatgcgg ctatcgcctg
     1981 gctgatgaag tcgtttggca atttccatca tgacgtgaca accgttctgc aaaactactt
     2041 tcattactgc gctctgaaaa tgagctgtgt agagctggcc cggacgtttg tctttctggc
     2101 taatcagggg aaagctattc atattgatga acctgtggtg acgccaatgc aggcgcggca
     2161 aattaacgcg ctgatggcga ccagtggtat gtaccagaac gcgggggagt ttgcctggcg
     2221 ggtgggacta ccggcgaaat ctggcgttgg tggcggtatt gtggcgattg ttccgcatga
     2281 aatggccatc gcagtctgga gtccggaact ggatgatgca ggtaactcgc ttgcgggtat
     2341 cgccgtcctt gaacaattga cgaaacagtt agggcgttcg gtttattaat gcagtctctc
     2401 gatccactct tcgcgcgttt atcccgttca aaatttcgct ctcgctttcg tctgggcatg
     2461 aaagagcgtc agtattgcct ggagaaaggc gcgccagtca tcgaacaaca tgcggcggat
     2521 tttgtcgcta aacgtcttgc tccggcatta ccggctaatg acggtaagca aacccccatg
     2581 cgcgggcatc cggtgtttat cgctcagcac gccaccgcga cctgctgtcg cggttgtctg
     2641 gctaagtggc ataacattcc gcagggtgtt tcgttaagcg aagaacaaca acgttatatc
     2701 gtggcggtaa tctatcactg gctggttgta tagatgaatc agccatgaga attgcggaaa
     2761 acgataaaat agcattccca gggtaccttt atttgccgcc gctgttagcg aacatgctat
     2821 cctttatgag aatttactta tcacgttgag ataattgcca cccgcaattg tatggacaga
     2881 taattaatgc atgtacaccc catttcaact ttccgtttgt tccaggaagg tcatctgcta
     2941 cgtaatagca tcgctatttt tgcgctaacc acgctgttct attttattgg tgcagagtta
     3001 cggctggttc acgaactttc tcttttctgg ccgctgaatg gcgtaatggc gggggtgttt
     3061 gcccgctatg tctggcttaa tcgactgcat tactatgcga tcagttatgt ggcgatgctg
     3121 gtttatgatg ccataaccac cgaatgggga ctggtttcac tggttatcaa tttctccaat
     3181 atgatgttta ttgttaccgt cgccttactg gtcgtgcggg ataagcgtct ggggaaaaat
     3241 aagtatgagc cagtaagtgc tttacggcta tttaattact gtctgattgc cgcattatta
     3301 tgcgctattg tcggggcgat tggttcggtc agtattgata gtctggattt ctggcctttg
     3361 cttgcggact ggttcagtga gcaattctca acgggcgtgt tgatcgtgcc ttgtatgctg
     3421 acgttggcta ttcctggagt gctgccgcgc tttaaagcag aacagatgat gcccgccatc
     3481 gcgtttattg tgtcggttat tgcctcggta gtcattggcg gagcggggag tctggcgttc
     3541 ccgctgcctg cattaatctg gtgtgcagtg cgctatacgc cgcaggtaac atgtctgttg
     3601 acctttgtca ccggtgcggt ggaaatcgta ctggtggcaa attcggtgat tgatatctcg
     3661 gtcggttcgc cgttctccat tccggaaatg ttctccgcac gtctcggtat tgccacgatg
     3721 gcgatatgcc caattatggt ttcttttagc gtggcagcga tcaatttgct aatgaagcaa
     3781 gttgcgctgc gagccgactt tgattttctg actcaggttt actcacggtc cggtctttat
     3841 gaggcgctga aaagtccatc gctgaaacag acacaacatc tgacggtcat gctgcttgat
     3901 atcgactatt tcaaaagcat taacgataac tatggacatg aatgtggcga taaagtgtta
     3961 agcgtgtttg cccggcatat tcagaagatt gtcggtgata aggggctggt ggcgcgcatg
     4021 ggcggcgaag aatttgctgt tgcagtgccg tcggtgaatc ctgtcgatgg tctgctaatg
     4081 gcggaaaaaa tccgtaaagg cgttgaactg caaccattca cctggcaaca aaaaacgctt
     4141 tatctcacgg taagtattgg cgtcggtagt ggtcgcgcat cgtaccgaac gctgaccgat
     4201 gattttaata aattgatggt cgaagccgat acgtgtctgt atcgctcgaa gaaagatggg
     4261 cgcaaccgta ccagcactat gcgttacggt gaggaagttg tctgacatct ttattcattc
     4321 cgcgcgattt gaatgcaatg tgttctacca ctttatgatc caggaaaagg cgtatttcct
     4381 cgattaaacc atgatccgcg ccacactttt ctcataaaac tgctcattaa tcatcaggag
     4441 ttgatcaaaa caggggttcg gcgtattaaa gtttgctaac ctgtcgcacc aattcaaacg
     4501 ggttgtatga ctaatcagaa gggaacccat tgtgaaaaca ctaaatcgtc gcgattttcc
     4561 cggtgcacag tatccagaac gtatcattca gtttggtgaa ggtaacttcc tgcgcgcctt
     4621 tgttgactgg caaatcgatc tcctgaatga gcacaccgat ctgaattctg gcgtggtcgt
     4681 tgttcgtccg attgaaactt cattcccgcc gtcactgagc acgcaggatg gtctgtacac
     4741 caccattatc cgtggcctga atgagaaggg ggaagcggtc agcgacgcgc gtctgattcg
     4801 ctcggttaat cgtgaaatca gcgtctacag tgaatacgat gaattcctga aactggcgca
     4861 caacccggaa atgcgctttg ttttctctaa caccaccgaa gcgggtatca gctaccatgc
     4921 gggcgataaa ttcgatgacg cgccagcggt aagctatccg gcaaaactga cccgtctgct
     4981 gttcgaacgc tttagccatt tcaacggtgc gctggataaa ggttggatca tcattccgtg
     5041 tgagttgatt gactacaatg gcgacgcgtt gcgtgaactg gtgctgcgct atgcgcaaga
     5101 gtgggcactg ccagaagcat ttattcaatg gctggatcag gctaacagct tctgttctac
     5161 gctggtggac cgtatcgtta ccggttatcc gcgcgatgaa gtggctaaac tggaagaaga
     5221 actgggttat cacgatggtt tcctcgacac cgctgaacac ttttacctgt ttgtgattca
     5281 ggggccgaaa tccttagcga ctgagctgcg cctggacaaa tatccgctca acgtgctgat
     5341 tgttgacgat attaaaccgt ataaagaacg caaagtggca atcctcaacg gtgcgcacac
     5401 cgcgctggtg ccggtggcgt tccaggcagg gctggatacc gtaggtgaag cgatgaacga
     5461 tgctgaaatt tgcgcattcg ttgaaaaagc catctacgaa gaaattattc cggtactgga
     5521 tttgcctcgt gatgaactgg aatctttcgc cagtgcagtc accgggcgtt tccgtaaccc
     5581 gtacattaag catcagctgc tgtctatcgc gctcaacggt atgaccaagt tccgcacccg
     5641 catcctgccg cagctgctgg cagggcagaa ggcaaatggc acacttccgg cgcgccttac
     5701 tttcgcatta gcggcattga ttgcgttcta tcgtggtgag cgtaacggag aaacatatcc
     5761 ggtacaggac gatgcacact ggctggaacg ttatcagcaa ctctggagcc agcatcgtga
     5821 ccgtgttatc ggtacccagg aactggtagc gattgtgttg gcagaaaaag accactggga
     5881 gcaggacctg acgcaagtgc ctggtctggt cgagcaggtt gctaatgacc tggatgcgat
     5941 tcttgaaaaa gggatgcgcg aagctgtacg tccgttgtgc taataacacc gttacttctg
     6001 aacccggttt cgaccgggtt tcttatttca ctatgttagt tacaacatcc tatattcctt
     6061 tatcattaac aggtgtcaca tgcagcgcct gttaatgttg taagcttgac
//
LOCUS       DAZPEU010000204         6076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPEU010000204 DAZPEU010000000
VERSION     DAZPEU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.206"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHU2L_003574"
     CDS             complement(<1..143)
                     /locus_tag="ACHU2L_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFR1358267.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACHU2L_003575"
     CDS             379..651
                     /locus_tag="ACHU2L_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR1358268.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHU2L_003576"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHU2L_003576"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR1358269.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1749
                     /locus_tag="ACHU2L_003577"
     CDS             1111..1749
                     /locus_tag="ACHU2L_003577"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1358270.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHU2L_003578"
                     /pseudo
     CDS             complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHU2L_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3132..3785)
                     /locus_tag="ACHU2L_003579"
     CDS             complement(3132..3785)
                     /locus_tag="ACHU2L_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1358271.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3785..5256)
                     /locus_tag="ACHU2L_003580"
                     /pseudo
     CDS             complement(3785..5256)
                     /locus_tag="ACHU2L_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5229..6076)
                     /locus_tag="ACHU2L_003581"
                     /pseudo
     CDS             complement(5229..6076)
                     /locus_tag="ACHU2L_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1623 c         1607 g         1419 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggttt gcgtggtttg cgtggatctt cggtgcgttg
     1681 tgctggatga cgacctttac gcgggtgtgg agtgggtatc tgacgctgaa gtcgctccag
     1741 cgtcagtaat tgcaatgtta tttgctactg cccgggccgc gttcaccggt tgcaaccgga
     1801 ttttttggat cgctgctcca ttcgtaccag ccgccgtcat acacggaaac attcttccaa
     1861 cccatggcgc gtgcgtacat aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat
     1921 gaaacttgct gatctggttt gatattccat gctttccaca tagcggtaat atcatcggcg
     1981 ctacgcatgg tgccatccgg gttatagaaa tcttccatat gcgtcgagtc gctaccagcg
     2041 tgtccccaac gtgctccggc tatttcacct tttggtttaa tatagctgta accgctggtc
     2101 gtaccgataa attctggcca cgaacgaatg ctcaccagcg atgcatcctg gcgatgcagc
     2161 agtccacggg cctgttccat atcgagcatc aactgcggtt gtgccgggat cttcacgccg
     2221 aaatccggtt ccgctttcac tttcggtggc gttcctcgct caacaggcag tcccgcgtcg
     2281 gaccaggttt gccagccgcc atccagcaga cgcacatctt tcacgcctgc gtagagcata
     2341 atttgcgcca cacgcgctcc agcgtatacg tcacgcccgt acagaatgac cgtggtgtca
     2401 tggcgaatgc cgtgttttgc cagcatcgct tttagttgtt catcagaaac tttgttccac
     2461 agcggttcgc tttccacttc gttggtatcg atgtagtcag cgccaggaat atggctgata
     2521 aggtaaagct taggagcgcc ctaggccgct tcaatgactt tccagtcacc gacaggtttc
     2581 gccgtaacct ctttaccttg ttgcaggtca tgcagccatt gcggataaac cagctgctca
     2641 aagtgcggca gtttttgtag acgggaaggt tcgcttagcg cgtcactcag aatggagata
     2701 tgcgtaaaac ctgctttttg cagtcgcgtt ttgacggcgt caacatcttt gccattaccg
     2761 tacagcgcca ccggggcatc ggctttcagg ttatgttgct tgatccacgc gttgagctgt
     2821 tcggtgctca ttttgtccag ccagctagca gagaggttta aggcggcagg ttcatgacca
     2881 gaagggccat ttaaggtttg tggccagccg ttataaaacg cgctggggcg agtatctatc
     2941 gctttgccat tttgttgctg aagttggtca agtgtaagag gcttcgccag ttcagcggcc
     3001 cacgaagaag cgcaagccag ccctaaagcc attgccagcg cggtcatttg agaaacacgt
     3061 ttcatcgaaa atcccgacat tgtagttttg agtgcgccat tctgaactga cgcattgaga
     3121 aaaactttgc gttatcgcag tttgttgtaa ttttctgacc actgcgccat atccagaaca
     3181 gaactatcag caggaacatc ctggagatcg tgcgttacct gaacgacggg gatcgccagg
     3241 gcgcgaactt cgctgaacac ccactggcga aaattatcgc gcagagccac atcaagacgg
     3301 ctgaatggct catccaggag taacgctttt ggctgggcga gaagggcgcg tagtagagca
     3361 acgcgcgctc gctgaccgcc agacaaagtg gcaggatcct gatggaaagc tccgtcaagg
     3421 cctgaacgct caagtgcatc attcacggca ttacgtcggg cattcccctt aagtgtcgcc
     3481 ggtagcgcca gcagtaaatt ttgcccgacg ctgaactggt caaataacag tgcatcctga
     3541 aaaagaatgc caatctgacg ctgtgcggtg ggtagcatgt caatccgttg ctcattgagc
     3601 catagctcac ctgtacaaga aaactgtccg gccagtgcac caatcatcca tgaaaacaga
     3661 gtggattttc cacagccaga cggccccatt aacgtgacga tgtcaccttt atcaaccgta
     3721 aagttaacgt ttgtcagcaa gcggctttct ggtaaacgta gcgaaacatt tttcacgcag
     3781 agcattagcg gattccttgt ctgacataac ctacccattt tgcgactaac gcggtcaggg
     3841 caaaaataat aagcggtaac aacagttgcc ataacgcctg ggcggcgaga ataccgttgc
     3901 tgccgccgct gcttaatgcc accgcctcag tggtgagcgt cggaaaacgc cccgcgccca
     3961 gccacagcgt cggcatatac tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca
     4021 gcacagggcg caacatgagt gggcatttca cgtaaaagaa gattttggcc cgcgaccagc
     4081 ccagtgtttg cgcaatcaaa attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga
     4141 taaacagcat ccacggcatt acccacagca gatgccccca gaccaccgcc gtccagcttc
     4201 catccaggtt cagccatagc gccagcgtgt actggcccgc caccagtggc agagcgggca
     4261 atagaatggg caaccatagc cacaactggc gacgctgtgc cccccattcc agccacagca
     4321 gtaacaggag caaagcgatg aatgttgcta ccagccccat tgtcagactg ttgatgagtg
     4381 cttcactatt gatcgtcgac tgatccgcga ggatcgccag cagaaccaca cacagcacac
     4441 cggttaaggg taaaaaactc gccagcgtag tgcctggtaa taaaggtgtg gcaggcttgc
     4501 gaatgccatc tacgcgggga atagtgcgcc gccagctgcg ccaaaacgta ggcggcgagt
     4561 aacagcatca gcagcagact ggcgagtgcg cctttcgttt gttgatccgc gtcgccctga
     4621 gttaaccact gccagctaat tactgccagc gtcggtggat tacctggccc aagaataatc
     4681 gccacatcca cgaccgaaag cgaccaggca acaatcgcca gcatcgccat tgccagcggc
     4741 tgtagcccag cgaatccagc acaatgacct gctgcaacag ccgtttttcg ctcaatactg
     4801 ccactaagat ccacagcaga aatgcgcttt ctttcactgc aagggtgagg cccagaccga
     4861 tccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca gccctccgtc
     4921 agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
     4981 cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa tcaccagcag
     5041 ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc
     5101 ctgcggtaac tgtggatcgg caaataacgc ctgccagcgt gtcaacgaca acgcaggcga
     5161 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag
     5221 caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc attcacccat
     5281 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga ttgcaatatt
     5341 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgcc ccagacaacg
     5401 ggatctgctt tacgcagttg cgcatcgggt gagagcagga aattggcaac taccttcgcc
     5461 gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc
     5521 tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc
     5581 gcatgtgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc
     5641 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
     5701 tcaagatatt gctacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct
     5761 tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg
     5821 gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat tggctttagc aaactccagt
     5881 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
     5941 tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca ctgaaaaatc tccccgcacc
     6001 ggcagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
     6061 aaattggcct ctttta
//
LOCUS       DAZPEU010000205         6065 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPEU010000205 DAZPEU010000000
VERSION     DAZPEU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.207"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            223..882
                     /locus_tag="ACHU2L_003582"
     CDS             223..882
                     /locus_tag="ACHU2L_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR1358272.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1083..1460)
                     /locus_tag="ACHU2L_003583"
     CDS             complement(1083..1460)
                     /locus_tag="ACHU2L_003583"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1358273.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1527..4493)
                     /locus_tag="ACHU2L_003584"
     CDS             complement(1527..4493)
                     /locus_tag="ACHU2L_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR1358274.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4496..5056)
                     /locus_tag="ACHU2L_003585"
     CDS             complement(4496..5056)
                     /locus_tag="ACHU2L_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1358275.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5182..5532)
                     /locus_tag="ACHU2L_003586"
                     /pseudo
     CDS             complement(5182..5532)
                     /locus_tag="ACHU2L_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(5735..>6065)
                     /gene="intI1"
                     /locus_tag="ACHU2L_003587"
     CDS             complement(5735..>6065)
                     /gene="intI1"
                     /locus_tag="ACHU2L_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1358276.1"
                     /translation="WFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITK
                     PATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDAL
                     PPLTSER"
BASE COUNT         1221 a         1678 c         1827 g         1339 t
ORIGIN      
        1 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
       61 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt
      121 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      181 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      241 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      301 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      361 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      421 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      481 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      541 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      601 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      661 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      721 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      781 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      841 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
      901 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
      961 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     1021 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     1081 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     1141 tgatcattta ttctgcctcc cagagcctga taaaaacggt tagcgccggg gttggatttt
     1201 tcagcgttcc agctaaggct aaggcattcc tgttcaagcg caagccgggc tataaagcgc
     1261 attatcgctt tacccgtgcc cttatttcga tcgcactgag aaacatacag ctctttaata
     1321 tgcagctgac cgctgtatcg gggcgaggga taaagaatat tgcaacatgc caggccagta
     1381 atgttattgc cgcagcgcgc tctgatcacc agggtaccgg aaagccggtt gaataacttt
     1441 ttacagagat aatccttcat caacgcttcc tgaatgatac cttcgccata ataggggtcg
     1501 tctcagaaaa cggaaaataa agcacgctaa gccggttgca gaggccgtag cggcctgaac
     1561 ttccccgcgc cgatcttggc gctgctgcgc cataggtaat caccggtcag gttgatgtgc
     1621 tcccagccga gtggcgacag gtactgcaat agcgagtcat cgacggcatg accattgccg
     1681 cgcaacgcat gcgccgcacg ctccaggtag accgtgttcc acagcacgat ggccgccgtc
     1741 accaggttga ggccgctggc ccggtagcgc tgctgctcga aactgcggtc acggatttca
     1801 ccaaggcggt tgaagaacac ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc
     1861 ccggcatgca cgcggcggcg tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg
     1921 cgctcgatgc ggcccaactc gcgcagcgcg acggccaagc cgttctggcg cgggtagctg
     1981 ccgagtttcc tgagcatcag cgaggccgtc accgtgccct gcttgatcga ggtggccagc
     2041 cgcaggattt cgtcccaatg ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc
     2101 ggcttgagcg cgtcataggc ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg
     2161 tcgcggatgc gcggcgcgaa gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg
     2221 gtgaagcccg ccgtgtcggt gtagtgctcc tcgatccgca ggtcggattc gtggtacagc
     2281 aggccgtcga gcacgtaggt tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat
     2341 ggcgcgtatt ggtcggagat gtgggtgtag aaagtccgtc ctgggctgct gccatatttt
     2401 gggttgatgt gccccgtgct ctttgccttg ctagcggttc ggaaattctg tccgtccgat
     2461 gatgatgtgg tgccatcgcc ccagtgcccg gcaaagggat gccgaaactg agcgttgacc
     2521 agttcagcca acgctgtcga gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg
     2581 agcttcgcgt aggtcgtgcc ggggcaggac tcggccatct tggtcaggcc caggttgatc
     2641 gcgtcggcca ggatcgtggt caacaacagg ttcttgtcct tggccagatc gcccgatttc
     2701 aagtgcgtga agtgccgggt gaagcccgtc cactcatcga cttcgagcag cagttcggtg
     2761 atcttgacgt gcggcaggac catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc
     2821 accgccgcat ccagcggcgt gatcttcaag cccgactcgg tgatgatggc atccggcagg
     2881 tcgttggctg ccgccatgcg gttgacggtg gcaagttgtg cttccagcag cgtcagccgc
     2941 tcatgcagat attgttcgca gtcggtggcc acggccagcg gcaattcgct ggactgcttg
     3001 aggctggtga acttctcggg cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa
     3061 ccctgcaccc agatgtcgcc cgagcgcagg gagttcttca actcggacag cgcgcacagt
     3121 tcgtagtagc gccggtcgat gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg
     3181 atgaagccgg tcggtgcatc ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc
     3241 agcacctcaa tggcatcaag cacgtttttg gcggcgggcg cggcccgcag cttgagcacg
     3301 gcaaggaatt ccggtgcata gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg
     3361 aaatcgaagt catcgggttg cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa
     3421 tcccaggaca tgacggcctc gatggcggca aacgcatcgc ggcctgattg cttggcgtcg
     3481 atcagcgcct gaccgatgcg cccgtacaga cgtaccttgg cgttgatggc cttgcctgac
     3541 gcctggaact gctgctgatg cttattcttg gcagcgttaa acagcttacc caggatgcgg
     3601 tcgtgcaggt cgatgatttc gtcggtgacg gtggccatgc cctcggtggc cagcgccacg
     3661 agagtggcgt agcgccgttg cggctcgaat ttggccaggt cggcgggtgt catctggccg
     3721 ccctcgcggg caatcttgag caggcggttc tggtgaacca gccgctcgat gccggtaggc
     3781 agatcgagtg cctgccatgc cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt
     3841 ggcttggccg gagactggcg caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc
     3901 ttgagcagat cgtcgaggcg gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg
     3961 tagatgcgcc ggttagcacg ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg
     4021 gcgggcagaa tgaccgactg ccgccgcagg tgcccgatca aggcgctggc cagcacgatg
     4081 cctttgtcgg tttgcatcgc cagctcggtc agcatctgga cggcctgccg gtaatggctc
     4141 atggtgaagg gccggaaacc gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc
     4201 tgctcccgct ggccgtactc gttccagctt tcgacgccga ccttgagctg gtcggcgacc
     4261 agcttcaaca agggcgggaa cggtagttca tcgacgccca ggatgacgcc gggaaagcgc
     4321 aggtaacaga gctgcaccgc gaagcccagc cgattggctg gcccgcgccg ctgtcggatg
     4381 atcgagaggt cggtatcgtt gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc
     4441 aacgccagca ggctttcccg ctcggcggcg gacaggatgg aacgacgtgg catatttact
     4501 gatccgttct caagtattga tacagggttt cgcgactgat tccgaattca cgagcaagct
     4561 tggtcttttg ctcgccagcc tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca
     4621 gggatttctt cctgccacgg taagccccgc gttgcttggc gagcgcaata ccctcgcgct
     4681 gacgctcgcg gatcagggcg cgctcgaact cggcgaacgc gcccatcacc gagagcatca
     4741 ggttcgccat cggagagtct tcgccagtaa aactgaggtg ttccttgacg aattcgatat
     4801 gcacgccgcg ttgtgtcagc gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca
     4861 ggcgatccat gctatgcacc accacggtgt cgccggtgcg ggcgaagctt atcagcgctt
     4921 ccagttgcgg acgcttgaca tccttgccgg atgccttgtc gctaaaagcg cgatcaacct
     4981 tgacgccttc cagttgccgt tccgggttct ggtcgaaggt gctgaccctg atatacccaa
     5041 tgcgctgtcc agtcatggaa ttccctgcaa aatgtcaggg aagactctat gaccttcaac
     5101 gagatatgtc aataaattca aaattcaatc ctatcctgac gcaatttaca catggcatct
     5161 gacatcaggt tagggtatgc ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac
     5221 gctggtttcg gtcgcagtgc tggcccgcgc ctggaagcgc tgatagcact ccagcccgca
     5281 gaaatgctcc acgtactcgg ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt
     5341 gcagcacacg cagcaactgg tgctcggttc attggcgttc atggtggtgt tcctccattg
     5401 gttgacgaag ccgacgaagg ccgccgccgg catcggcctg gcgaacagga aaccctgcac
     5461 cgtgtcgcaa cccgcctgcc gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc
     5521 taccacctcc attgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
     5581 tatagcagcg gaggggttgg atccatcagg caacgacggg ctgctgccgg ccatcagcgg
     5641 acgcagggag gactttccgc aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg
     5701 ggtagtgaat ccgccaggat tgacttgcgc tgccctacct ctcactagtg aggggcggca
     5761 gcgcatcaag cggtgagcgc actccggcac cgccaacttt cagcacatgc gtgtaaatca
     5821 tcgtcgtaga gacgtcggaa tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc
     5881 tgcggagcaa ggccgtcgcg aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga
     5941 tgcctgcttg ttctacggca cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat
     6001 ggcgacgcac gacaccgctc cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga
     6061 accac
//
LOCUS       DAZPEU010000206         5829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPEU010000206 DAZPEU010000000
VERSION     DAZPEU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.208"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..948
                     /gene="lpfD-O113"
                     /locus_tag="ACHU2L_003588"
     CDS             <1..948
                     /gene="lpfD-O113"
                     /locus_tag="ACHU2L_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR1358277.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
     gene            1196..2236
                     /gene="pstS"
                     /locus_tag="ACHU2L_003589"
     CDS             1196..2236
                     /gene="pstS"
                     /locus_tag="ACHU2L_003589"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR1358278.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2323..3282
                     /gene="pstC"
                     /locus_tag="ACHU2L_003590"
     CDS             2323..3282
                     /gene="pstC"
                     /locus_tag="ACHU2L_003590"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR1358279.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3282..4172
                     /gene="pstA"
                     /locus_tag="ACHU2L_003591"
     CDS             3282..4172
                     /gene="pstA"
                     /locus_tag="ACHU2L_003591"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR1358280.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4264..5037
                     /gene="pstB"
                     /locus_tag="ACHU2L_003592"
     CDS             4264..5037
                     /gene="pstB"
                     /locus_tag="ACHU2L_003592"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR1358281.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5052..5777
                     /gene="phoU"
                     /locus_tag="ACHU2L_003593"
     CDS             5052..5777
                     /gene="phoU"
                     /locus_tag="ACHU2L_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR1358282.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1446 a         1399 c         1520 g         1464 t
ORIGIN      
        1 tcgggatatc ttattaccgc gaacgaaaat aaggttggaa cgacatttaa caccactgta
       61 actaatggat cctcatatct tggacgctgt cattgtgata ctggtaacgt aggagaattc
      121 ccctatatct attatacgtc aaaaataaac caggcattaa cttatgctgg ggttcactct
      181 aatattaatt attatgatct taatccgaac cttgatgttg ggattgctat agatattctt
      241 ggggttgggt atgtgaatgc accttttgaa tatcatgcta acaacccgtc tggtaataca
      301 aagtacaatt gcaaccgcat tgaaccttta agtatatcca gtggggctaa agcgatagta
      361 tatttttata tcaagaagac atttgcagga aaattgatta ttcctgaaac aaagatagtg
      421 acattgtatg gaacaattag ccgtgacacc ccggtggatt actcacagcc gatggctgat
      481 gtttatattc ggggcgatat tacagctccg caaagttgcg aaataaataa tttaaagcca
      541 gtttgttttg attttaaaga aataccagct gcagattttt catctgttgt tggaagtgcg
      601 gtaacaacac ataaaattac taaaacagtc actattgagt gtgaaaacct ggggatactg
      661 aataccgatg atatcagtac gtctttttat gctaccgaac ccaatacgga caattcaatg
      721 gtcgtaactt caaactctaa cgtggggata aaaatttacg acaaaaataa taaggaaatc
      781 aaagtgaacg gtggtgagtt gccaacagac atggataaat caacagtgta tggtgaaaaa
      841 tcaggtagtg taactttttc agctgctcct gcgagcctta ctggagctcg cccagccccg
      901 gggcaattta ctgcaacagc gacgataact gttgagattg tgcgctaata ggtctgacaa
      961 cgattgttca tcacatcgtt gtcatctcac ccatttcttt atcactttta aatgcatcat
     1021 gacaaaaaga tggcatgtgg aattttcatc ttcacattta aaatcagtaa gttatttggt
     1081 aattcctttc tgtcataaaa atgtcatatt ccttatatat aactgtcacc tgtttgtcct
     1141 attttgcttc tcgtagccaa caaacaatgc tttatgaatc ctcccaggag acattatgaa
     1201 agttatgcgt accaccgtcg caactgttgt cgccgcgacc ttatcgatga gtgctttctc
     1261 tgtgtttgca gaagcaagcc tgacaggtgc aggtgcaacc ttccctgcgc cggtgtatgc
     1321 caaatgggct gacacttacc agaaagaaac cggtaataaa gttaactacc agggtatcgg
     1381 ttcttccggt ggcgtaaaac agattatcgc taataccgtt gattttggtg cctctgacgc
     1441 gccgctgtct gacgaaaaac tggctcagga aggtctgttc cagttcccga ccgtgattgg
     1501 cggcgtggtg ctggcggtta acattccagg gctgaagtct ggcgaactgg tgctggatgg
     1561 taaaaccctc ggcgacatct acctgggcaa aatcaagaag tgggatgatg aagccatcgc
     1621 caaactgaat ccgggtctga aactgccttc acaaaacatt gctgtagtac gccgcgcaga
     1681 tggctccggg acttccttcg tcttcaccag ctacctggcg aaagtgaacg aagagtggaa
     1741 aaacaacgtt ggtactggct ctaccgtaaa atggccgatc ggtctgggcg gtaaaggtaa
     1801 cgacggtatc gccgcgttcg ttcagcgtct gccgggtgca attggttatg ttgaatatgc
     1861 ttacgcgaag cagaacaacc tggcgtacac caaactgatc tccgctgatg gtaaaccggt
     1921 tagtccgacc gaagaaaact tcgctaatgc agcaaaaggt gcagactgga gcaaaacctt
     1981 cgctcaggat ctgaccaacc agaaaggcga agatgcatgg cctattacct ctaccacgtt
     2041 cattctgatc cacaaagatc agaagaaacc agaacaaggc acagaggtgc tgaaattctt
     2101 cgactgggcg tacaaaaccg gggctaaaca ggcgaacgac ctggattacg ccagcctgcc
     2161 ggatagtgta gttgaacagg ttcgcgctgc gtggaagacc aatattaaag acagtagcgg
     2221 taagccgctg tactaataaa actccaggcc gggtacggtg ttttacgccg catccggcat
     2281 tacaaaatga ctttgtaaac gcgtttaact gaagagtaac ttatggctgc aaccaagccc
     2341 gcttttaacc caccgggtaa aaagggcgac ataattttca gcgtgctggt aaaactggcg
     2401 gcgctgattg tgctattgat gttgggtggc attattgtct ctctgatcat ctcctcctgg
     2461 ccgagcattc agaaatttgg tctggctttc ctatggacca aagagtggga tgcaccgaac
     2521 gatatctacg gggcgctggt gccgatctac ggtacgttgg tgacttcgtt tatcgcgctg
     2581 ctgatcgccg tcccggtgag tttcggtatc gccctgttcc tgactgagct tgcgcctggc
     2641 tggctgaaac gcccgctggg tatcgccatt gagctgctgg cagccattcc aagtatcgtt
     2701 tacggcatgt ggggcctgtt tatctttgcg ccgctgttcg ccgtttactt tcaggagccg
     2761 gtcggcaata tcatgtcgaa tatcccgatt gttggcgcgc tgttctctgg ccccgcattt
     2821 ggtatcggta tcctcgcggc aggcgtgatc ctcgccatca tgattattcc gtacattgcg
     2881 gcggtaatgc gtgatgtgtt cgaacaaacc ccggtgatga tgaaagagtc ggcctacggt
     2941 attggctgca ccacctggga agttatctgg cgtatcgttc ttccgttcac caaaaatggt
     3001 gttatcggcg gcatcatgct ggggctgggc cgcgcgctcg gtgaaaccat ggcggtgacc
     3061 tttatcatcg gtaacaccta ccagctcgac agcgcctcgc tgtacatgcc gggcaatagt
     3121 atcacctctg cgctggcgaa cgaatttgcg gaagcggaat ccggtctgca cgttgccgca
     3181 ctgatggaac tgggcctgat cctgtttgtg attaccttca tcgtcctcgc cgcatcgaag
     3241 tttatgatta tgcgcctggc taagaatgag ggggcacgct aatggctatg gttgaaatgc
     3301 aaaccactgc ggcgctggct gaatctcgcc gcaaaatgca ggcgcgtcgc cgcctcaaaa
     3361 accgtattgc gctgacgctc tcgatggcga cgatggcctt cggcctgttc tggctgatct
     3421 ggattttaat gtccaccatc actcgcggta tcgacggtat gtcgctggcg ctgttcactg
     3481 aaatgacgcc gccgcccaat acggaaggtg gtggtctggc gaacgccctg gcgggtagcg
     3541 ggctgttaat tttgtgggcc acggtattcg gtacgccgct gggcattatg gcggggattt
     3601 atctggcgga atatggtcgt aaatcctggc tggcagaagt gattcgcttc attaacgaca
     3661 ttctgctctc cgcgccgtcg attgtggttg gtctgtttgt ctacaccatc gtggtggcac
     3721 agatggagca cttctccggc tgggcgggcg taattgcgct ggcgctgttg caggtgccga
     3781 tcgttatccg caccactgag aacatgctga aactggtgcc ggacagcctg cgtgaagcgg
     3841 cttatgcgct gggtacaccg aagtggaaga tgatctccgc gattacgctg aaagcgtcgg
     3901 tgtccgggat tatgaccggt atcctgctgg cgattgcccg tattgccggt gaaaccgcgc
     3961 cgctgctgtt taccgcgctc tccaaccagt tctggagcac ggacatgatg cagccgatcg
     4021 ccaacctgcc ggtgacgatc tttaagtttg cgatgagccc gtttgcggaa tggcaacaac
     4081 tggcctgggc cggggtattg atcattaccc tgtgcgtcct gctgctgaac attctggcgc
     4141 gcgttgtttt tgcgaagaat aaacacggtt aacacttttc cggatgcggc gtgaacgcct
     4201 tacccggccc tacggtaagc ctgattagcg aaaagcatca ggtacgatga ggaaaagatt
     4261 gcaatgagta tggttgaaac tgccccgagt aaaattcagg ttcgtaattt gaacttctac
     4321 tacggcaaat tccatgccct gaaaaacatc aacctggata tcgccaaaaa ccaggttacg
     4381 gcgtttatcg ggccgtccgg ctgcggtaaa tcgacgctgc tgcgtacctt caacaaaatg
     4441 tttgaactgt acccggagca gcgtgcggaa ggtgaaattc tgcttgatgg cgacaacatc
     4501 ctgaccaact ctcaggatat cgccctgctg cgtgcgaaag tgggcatggt gttccagaaa
     4561 ccgacgccgt tcccgatgtc catctacgac aacatcgcct ttggcgttcg tctgtttgag
     4621 aagctctccc gtgccgacat ggacgagcgc gtgcagtggg cattgaccaa agccgcattg
     4681 tggaacgaaa ccaaagataa attgcaccag agcggttact ctctctctgg tggtcagcaa
     4741 cagcgtctgt gtattgcgcg tggtatcgcc attcgcccgg aagtgctgct gctcgacgaa
     4801 ccgtgttcgg cgctcgaccc catctctacc gggcgtattg aagagctgat caccgaactg
     4861 aagcaggatt acaccgtggt gatcgtcacc cacaacatgc agcaggctgc gcgttgttcc
     4921 gaccacacgg catttatgta cctgggcgaa ttgattgagt tcagcaacac ggacgatctg
     4981 ttcaccaagc cagcgaagaa acaaacagaa gactacatca ccggtcgtta cggttgattc
     5041 aggagtgcgt tatggacagt ctcaatctta ataaacatat ttccggccag ttcaacgccg
     5101 aactggaaag tatccgcacg caggtgatga ccatgggcgg catggtggag cagcagcttt
     5161 ctgatgcaat caccgcgatg cataaccagg acagcgatct ggcgaagcgc gtgatcgaag
     5221 gcgacaagaa cgtcaacatg atggaagtgg cgatcgatga agcctgcgtg cgcattatcg
     5281 ccaaacgtca gccgacggcg agcgacctgc gactggttat ggtgatcagt aaaaccattg
     5341 ccgagctgga gcgtattggc gacgtggcgg acaaaatctg ccgtactgcg ctggagaaat
     5401 tctcccagca gcatcagccg ttgctggtaa gtctggagtc gctgggccgt cataccatcc
     5461 agatgctgca cgacgtgctg gacgcgttcg cgcggatgga cattgacgaa gcggtacgta
     5521 tttatcgtga agataaaaaa gtcgatcagg aatacgaagg tattgttcgt caactgatga
     5581 cctacatgat ggaagattcg cgcactattc cgagcgtact taccgcgctg ttctgtgcgc
     5641 gttcaatcga acgtatcggc gaccgctgcc agaatatttg tgagtttatc ttctactacg
     5701 tgaagggtca ggatttccgt catgtaggcg gtgatgagct ggataaactg ctggcgggaa
     5761 aagatagcga caaataattc accagacaaa tcccaataac ttaattattg ggatttgtta
     5821 tatataact
//
LOCUS       DAZPEU010000207         5823 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPEU010000207 DAZPEU010000000
VERSION     DAZPEU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.209"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..130
                     /gene="ymgI"
                     /locus_tag="ACHU2L_003594"
     CDS             <1..130
                     /gene="ymgI"
                     /locus_tag="ACHU2L_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR1358283.1"
                     /translation="IIPIITATLILNYLNNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            132..476
                     /locus_tag="ACHU2L_003595"
     CDS             132..476
                     /locus_tag="ACHU2L_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR1358284.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            480..815
                     /locus_tag="ACHU2L_003596"
     CDS             480..815
                     /locus_tag="ACHU2L_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR1358285.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(872..3193)
                     /locus_tag="ACHU2L_003597"
     CDS             complement(872..3193)
                     /locus_tag="ACHU2L_003597"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1358286.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(3310..3519)
                     /locus_tag="ACHU2L_003598"
     CDS             complement(3310..3519)
                     /locus_tag="ACHU2L_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358287.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(3919..4138)
                     /gene="ymgF"
                     /locus_tag="ACHU2L_003599"
                     /pseudo
     CDS             complement(3919..4138)
                     /gene="ymgF"
                     /locus_tag="ACHU2L_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(4270..5793)
                     /locus_tag="ACHU2L_003600"
     CDS             complement(4270..5793)
                     /locus_tag="ACHU2L_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1358288.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         1618 a         1350 c         1252 g         1603 t
ORIGIN      
        1 cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata gcgagcgttc
       61 tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt acttaatacc
      121 ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca ttgttctgct
      181 ccacgccagc gatagcggat atgaaccgca ccacgaaagg tgcattgcta ggcgcaggcg
      241 ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct gttggcgctg
      301 gtgttagtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag
      361 tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc gaaggggcga
      421 ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa
      481 tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca atgacctggg
      541 cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact tgcgcagaag
      601 ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg gtaatgggta
      661 atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg agcgccaaag
      721 tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg atcacggcaa
      781 ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgtacc gcgtcgatga
      841 atacgacgcg gttttttagc taacggcggg atcagaactt atatttcagg cccaccatca
      901 ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc cacattcccc cagacgctgg
      961 ccgctggatt caggttgcca tttacgccaa gtttaatttc ccccaaatta ctcaccccat
     1021 cttgttcgac tttcacaccg ttcattgaga cagcaaaatc tttactgtta tggatccagt
     1081 tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc gctgtttttc tcctgagtac
     1141 gaataaaggt gcggaagccg agtcgggtct ggatgttgtt atttgcgtca ttctcaatac
     1201 gtgagccatt ggcttccgtg tgcgcattat gatcgacgcc catccagata gcctgtgcct
     1261 gtggctggaa gatccacgta tatttcgccg cttccagacc aaaggattta ttcagggcaa
     1321 aaccgtaacc cgcttccagc gagacggttg cacctttcgc gctataggat tcataggcta
     1381 attcatcccc tttcacggag tttttaaacc agctatattg cgcccaggag tcaatgtatg
     1441 cgcctttttt actgatgtca tcggcaaacc aagtggcata cagccctgcg ctatagcctc
     1501 tgacactccc tttcgaacga taatccgaca cgctggaatg agttaagttg tagtcgcggg
     1561 cataaccagc catcactccc aaacgccaac tgtcactatc ggtaaaacca ccggttaaca
     1621 gatcgccccc taactgcgag acgtagcgat gcgaggtcgt tctcaactgt ccgttgctgt
     1681 cacgccaggc attatgcccg ccaatttgac gtagccaaag tcggcttgaa cgctcctgtt
     1741 cagttacagg atcgatgtag cgcgtttcac ccgcacggtc gttcagacgc ataacaaaaa
     1801 gagtgttagc ggctgccagg ttggcgacgt aacttccagc ttcggggcgc aggacggggg
     1861 taggcgcaac agtaggcgct gaacttcctc cggagtttgt ttcaccgccg ctcgttgcct
     1921 ggaagttagt cagataccag tttttattgg tcgcttcggt attacctcgg gttaatgaat
     1981 attcgtaggc accggcgaaa atcctcgaat ccttaataaa ttccccattt gattccccct
     2041 caacgctgat aatctctatc ccatttaacg tgtaagctcc gctgcctcct gcattggtaa
     2101 ctttaactcg ggttgttcca gaggtatttc cattaataac gactttatcc gtagctgaat
     2161 tatcatcatc caattcagtt ctgagatgca atacgccatt attaccaacg taattttcag
     2221 ttattgttaa tcttgttggt tcgacatccc gtccatctgc ccgggaaata tagaccgtac
     2281 tattatcaac cgtgaggttg ctcaccgttg agtcagcttt aatatcccag gtactattat
     2341 ctgaaagcga cagataggta tgagtgttat catctgttga aatattagct gaacccgtta
     2401 tttttgaatt agttgcatcg acggtcaaat gacctttaat attattgata aaaacatctg
     2461 cagccgatga tacctcacta tccgtaatgg tcacgttact cgtctcatct ttatggtaga
     2521 ggatttcatt tgctgaccgt agtgagctac cagataaaat atctaccgtt gcttcctgac
     2581 catttatgga agactccagc acaattccag acccctgatg cgcgactgca gatgtatttt
     2641 tcagcgtcac cgtgccgcca ttataaattt tagcaatcgc ggcgtcagga cccgttgtat
     2701 aaatttctcc ctggttaaat gaaactgtcc cctggcgggc agcgatggca tgagcattgt
     2761 ccccatgcgt ggtgatatca acatcggtta atgtaacgct ggcattggta taatcaagcg
     2821 tgtcaccagc cttgacgaca gtgccattag cgtacgcggc ataactttct gttccgtggg
     2881 ttgtgatctc cccttttgcc atatccagtt ttgctatggc attattttca ttacttcctg
     2941 tatcggcagt actttcttca tcaagagaag atacacacgc acacgcgctt ccgggagatg
     3001 cacacgcaag aacgccgact gcgtgattac ccgcagtaac aataaatgta tcatctccgt
     3061 cagaaaaaga gggggtaacc gaaccgtcag agttgactct tattatggtg tcagtattca
     3121 ggtttccact cgtttgactt agcaaaccaa ttgagtaatc ccccgttgtg acaatcgtgt
     3181 ttttaccctg caaaataaca gcaccgccaa attcacttac gattgcgcta gctgttaatg
     3241 ctgggcttag aggactattg tcagaaggat ataaaccgcc cgttgttaca atgacgccat
     3301 tgttaatggt taacttacta ccctctcctg caacggatac ccgtaacttc cattacccgt
     3361 tgttataact agcgcattat caatatcttt tacttttcca tcttttattg ccacagcatg
     3421 tttatattca tcgtgctgaa taaactgttc agcatatgca ttacccaccg ccagagcaat
     3481 aagtcccaaa ctaaacccgg ggagtttttt cagtttcata gattaactat ccattcattg
     3541 gattaacaaa cattcctggt caaataacct atgagctcag attaccaaaa cggagggtaa
     3601 agacaaaaac aaaagcacaa aaaatttaaa tagataaaca gcgacaatat ataaatgata
     3661 aataagtcgg cattatcatt tttcacctta ttttcatgac attgatcact ttggggtgac
     3721 tattttgttt aacaaataac tcttcgacat caaaattatt aatagttgcc atggtaatta
     3781 tttcggtgat atataaaaac aactgtgata acaccaaggt tgacaactat ttgcgcaata
     3841 attcctttcc ttcaatgatc caggcgttca ctgccagcaa cccattcagc cgcatactga
     3901 cagtgagaaa ctgaaagatt attgctgatc cctgtggcta acgagtcttt ctcttaaatc
     3961 gattaaccag attgatcgcc agccaaacaa gggcattaag caaatttatc gtcacaagca
     4021 cttctcccag cccggtactg ggttttgcaa taaggatgac caggagggtg atcagcataa
     4081 atgcaatgga aaagatgata tagagagtga aataatccag attattacta ttattcatgc
     4141 tactaatggc aatcctgtca aaactttcaa acaattatag caaacaaaat aatgaacaat
     4201 agtaacaaga ggcaacgagc taattagctg tctataatta caccgcacta tggccttact
     4261 gatcaatttt cactcaacca caaccttcac cttcggttta gaaaggataa ttttgaccag
     4321 gtcgatgtat gtaacaggtt tatagaaata ataaccctgc tgaaatgtga tattattttg
     4381 gttcagatag ccaagttgtt ctttcgtttc gacaccttca gcgacgatac tgatcgaaag
     4441 tttacgcgcc agatccaata cacaatccag aattcgggtt gattctgggt ctgcgtttac
     4501 gcggccaacg aaactgtgat caattttgat ataatcaata tgcaggtcat gaagataaga
     4561 aagccctgag tagccagtac cgaaatcatc cagcgcgatg acaaaaccat tttcatgcag
     4621 aatgttcaac cgctgaacca gactttcatc aacattcaat ggctcacgct cggtgacttc
     4681 cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg aaatttaaac actcgtcgac
     4741 aaacgtcggc gaaataatat gcgaagcgct aaaattaatt ccaatatgaa aaccttccgg
     4801 cagtttactc gcgatagcgt tcatctgtct ggcaacctga ttcatcaggc tttgtgtaag
     4861 cggaacgatt aatccggatt tttcagcaag tggaataaat gccgcgggtg aaatatatcc
     4921 accgtgaggt tgtttccagc gggctaacac ctcaactccc cgcaatgtcc cttcccgacc
     4981 atttaccaca ggttggtaaa aagggacgat ctccccatta tttattgccc gtcgcaggat
     5041 ctcttcaggc gttgcgcttt tattgaaata tctatcaagc aggaaggcag cggcgctggc
     5101 gattaacaga ataaatatta aaatcccaaa gcctttatcg ataagtctgt agaagctaaa
     5161 gagtggcggc gggttaaaga taatagtgaa atgatatttt gtggagtgaa ccttctcggt
     5221 atagggataa tgtccattaa gcgtcatcac atcagccgtt ggtccaataa tgtcatccgc
     5281 cacgcgtaat acatacctta cccctttcaa gggtacatta agcgccccgc gaatatgttg
     5341 atcacttatc gtaaccaaaa tgcgcgtatc tgcaaattgg ctctgataga gcaggatagg
     5401 taatctatta acggtgtcga ttgccggagc caacagtaaa ttactgtccg ggacaacagg
     5461 aatacgagac agcaggaccc gattcccagg cagggatgta caccagacta ttccctgttt
     5521 gagaatgatg attgtgcgca ggtgaggttt cagagctgct tcagtgccaa gctgatattg
     5581 ctcctcgagg tcgcattcct tcccggcaat gttaaccgct gtttgagtcg cctgtgacgc
     5641 ttcttcaagt atatgattga tattgttggc ggcatatctt gctcccgcca ggtactctgc
     5701 ttttgcggaa taccagagct ggatgttcaa aatagcgaca ccggtaataa acaagcatat
     5761 cgcaacgagg atgggtatga gtgtattgcg catttgcttg tcccggccaa agggtttaat
     5821 aca
//
LOCUS       DAZPEU010000208         5796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPEU010000208 DAZPEU010000000
VERSION     DAZPEU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.210"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            76..1008
                     /locus_tag="ACHU2L_003601"
     CDS             76..1008
                     /locus_tag="ACHU2L_003601"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1358289.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(1049..2479)
                     /locus_tag="ACHU2L_003602"
     CDS             complement(1049..2479)
                     /locus_tag="ACHU2L_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1358290.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLAYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(2634..3260)
                     /gene="dsbA"
                     /locus_tag="ACHU2L_003603"
     CDS             complement(2634..3260)
                     /gene="dsbA"
                     /locus_tag="ACHU2L_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR1358291.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(3277..4263)
                     /gene="srkA"
                     /locus_tag="ACHU2L_003604"
     CDS             complement(3277..4263)
                     /gene="srkA"
                     /locus_tag="ACHU2L_003604"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR1358292.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(4340..4609)
                     /locus_tag="ACHU2L_003605"
     CDS             complement(4340..4609)
                     /locus_tag="ACHU2L_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR1358293.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            4679..5263
                     /gene="mobA"
                     /locus_tag="ACHU2L_003606"
     CDS             4679..5263
                     /gene="mobA"
                     /locus_tag="ACHU2L_003606"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR1358294.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            5245..5772
                     /gene="mobB"
                     /locus_tag="ACHU2L_003607"
     CDS             5245..5772
                     /gene="mobB"
                     /locus_tag="ACHU2L_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR1358295.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1522 a         1348 c         1301 g         1625 t
ORIGIN      
        1 ctccttaagg atcatccgtt atttgggtca ttttctgcta aaatctgcgc cttgcgcttc
       61 tttagtgcac tgtctatggc taaccttttg aataaattca ttatgacgag aatactcgct
      121 gcgataaccc ttttgctgag tatcgtattg accattttgg tcactatttt ttgttccgtc
      181 ccgatcatca ttgccgggat tgtaaaacta ttgctacctg tgccagtcat ttggcgaaag
      241 gtttcgcgtt tttgtgattt tatgatgtat tgctggtgtg aaggtctggc ggtattactg
      301 catctgaacc cacacctgca atgggaagtt cacgggctgg aagggttaag taagaagaac
      361 tggtatctgc ttatttgtaa ccaccgtagt tgggcagata ttgtcgtact gtgcgtgctg
      421 tttcgtaagc acattccgat gaataaatat ttcctcaagc aacagctggc ctgggtgcca
      481 tttcttggcc tggcgtgctg ggcgctggat atgccgttta tgaagcgtta ttcccgcgct
      541 tatttattac gtcatccgga gcgacgtggt caagatgttg aaaccactcg gcgttcttgt
      601 gaaaagtttc gcctgcatcc caccaccatt gtcaatttcg ttgaaggctc ccgtttcacg
      661 caagaaaaac atcagcaaac ccactccact tttcaaaact tgttgccacc aaaggcggca
      721 ggcattgcga tggcgctaaa tgtactgggt aaacaattcg ataaactgct taatgttacg
      781 ctgtgttatc cggacaataa ccgtcagccg ttcttcgata tgttaagcgg taaactgacg
      841 cggattgtcg tacatgtgga tttacagcct attgccgatg agttacatgg cgactacatc
      901 aatgacaaaa gctttaagcg ccattttcag caatggctga actcattgtg gcaggaaaaa
      961 gaccggcttc tgacgtctct catgtcatca cagcgtcaga ataagtaagt tttactcgcc
     1021 tctgaatgag cagaggcgag tgagtatttt aatgaacaaa acgtccggca cgagacataa
     1081 attcttcttc gctcatctcc tgtccgttaa aaacaacttt acccggtgta taacgcagct
     1141 gcagcgaggc ggtattgttg tcaatcgtga ttaactgaaa catctgacct aatgtcatca
     1201 tcccgctgat ttgtttatca gcctgctttt gcgctttttc cgcatccatt ccttcagata
     1261 aattaagctg ttttgcggtt tctgtgacga cattaagcgg taacttcaca tcaaaattga
     1321 gcgatttgat atctttgttt gtggatgatg aagatttggc tgggtcggca atactgatat
     1381 ccagattggc atttagttcg ccgagtgcgt tcttccatct taccggttgt ttaatggtcg
     1441 gctcactttt ttgtaataac ggtaaatact ctttgaacaa tgcagcattc acttcttgca
     1501 gagcaacttc atcgtttgct aactcagggt gtgcagcaag ctgcttctgc atcgcaatgt
     1561 tatactggat gataaattgg cgcactgctg agggatcaat agattcagca atcaatgacc
     1621 actcaccact accgagttgc tgattccctt ttgttaactt atcaagttcg taggccagat
     1681 tgacattgat ataatctttg tctttaacgc gatccagcct ggtttttgca acgaaagcat
     1741 cgatttgggc aacgtcttcc ccgtgattga taatgttaat ctgattaaat ttcgactcgc
     1801 tttcaccaac cggaaatttc ttttcttcta atcgcgccag agaatcaatg gtaaaacttt
     1861 ttgccgttac tttgttcatt ggtgataatt gcagaacaat atcttcgact ttgccttcaa
     1921 tatccaggtt agacaatgaa ctaccatcac cattaaaagt aaactggccc tcaccaaaag
     1981 aaaattttcc atactctgca ggggcaacat ctaatgtcgt agagaatgag ccaccaaacg
     2041 cgaatcgcaa tgtcgcttcc acgggcgatt tatttttcgc ggcgataaat agcggttgtg
     2101 tcagttcatt attcactaag ttcacttttg ccgctgccag tgctgggatg acattaccat
     2161 gcattaacat tgtgatgggc aacggaccgt gctcaacatc cacatcaaat acaactttct
     2221 ggcctggctt gatattgaga tcgggtgcac cgttatcgaa tgtcatttgc atttgaaaac
     2281 gggaattaaa aaagcctttg tcaaagtttt tataacttaa cgtcatatcg taggcatgtt
     2341 cacctttctt tttcgcatcg ttaaaatctt taataaactt ttcgatacca ggctgaattt
     2401 gcgtacctgt gtaccatgtg ccaccacccc agattacggc taacgcaaca ataacacctg
     2461 tagctgactt acgtatcata taaatattcc ctgataaaac agcaaaactt tgaatatcca
     2521 cttatgctga aataatgtcc agcggcagga tgcattatca gaatgtattt atatttatta
     2581 aataataaaa aaagcccgtg aatattcacg ggctttatgt aatttacatt gaattatttt
     2641 tcctcggaca gatatttcac cgtatcagca tactgctgaa caaaaacatc catattgctg
     2701 gtatccatac cctgcggatt cagctgatat ttaccgttaa caaacatcgc cggaacacca
     2761 cgcaattgca catcagctgc ggctttttcc tgctgagcga ccagagattt caccacgaag
     2821 ctgttccacg ccgcgtcgta ctcttcacct ttaatacctg cattgataaa tacatcgcgg
     2881 atatcagatg ctgaacgaat ggtctgggtt ttctgtacgc cttcaaacag cggaacagtc
     2941 actttgtctt ccacgcccag cgccatcgcc acagcccatg cctgagtcag atctttgccc
     3001 aggtcaccac ccatgaagtt gacgtggtat ttagtcatct tcacgccttc cggcagtttt
     3061 ttcttcacat tatcagaaat atgcagaact tcttcaaact gatagcagtg cgggcagaag
     3121 aaagagaaaa actccagcac ttgcggcgcg ccagctaccg gtttttccag ggtagtgtac
     3181 tgtttaccat cttcatactg cgccgccgat gcgctaaacg ctaaaactaa accagccagc
     3241 gccagccaaa tctttttcat gatctactct ctccgattaa tacataggtg ttaattgcaa
     3301 agggggttct tgtagaactt ttgcctgttc tataaaagtc gccgtctgtc gtagccagta
     3361 atcttccccg gttaaccacg ggaaattttt cgggaacgcg ggatcagccc aatgccgcat
     3421 tagccaggca agataataaa ccaaacgcat ggcgcgtaaa ggttcaatca gtccgatttc
     3481 agcggtgtcg aactcgctaa attcttcata agcttcaata atagtttcca gttgcatccg
     3541 ctgctgggct ttatcgccat tgagcaacat ccacaaatcc tgaatagctg gaccattacg
     3601 tgcatcatcc agatcaacaa acattggacc atcgcgccag agaatattcc cggcgtggca
     3661 gtctccatgt agccgcagaa cggtgaaatc ttcccgccag tgtgctgtaa cggcggcgat
     3721 cagctcatct gtcgctttca gaaatgccgc tttcaacccg gaaggtatca gtgtagcgtc
     3781 ctcaaacagc ttgcgaggct caatgagata ttcattcaga ccgatggtcg ggcgatgaat
     3841 aaaaagctgt ttgcgccccg tctggtgcat acgccctaaa taacgcccaa ccgcttccat
     3901 ctgatcgata ttatcagctt cgaactggcg accaccgacg cttggaaaaa cagcgaaata
     3961 aaatccctga tgattcaata aagtctgacc gttaaaggcc acaggtgctg cgaccggaac
     4021 ttcatcattt accagctgca acgcaaattg atgttcttcg aggatttgat cggctgtcca
     4081 acgttcaggg cgataaaatt tgacgacaaa acgtcgacgc tcttcgtcct gaaattgata
     4141 gacacggttt tcatagctgt taagcggggt aagaccggaa tccacccgga tcccttgctt
     4201 aaacagagcg tccatgatgg tatccgggtg tagtgtctgg aaagtaaaag cgctgttgtt
     4261 catcccatca tccggaaaac attacgaatg atgaaggata tcatttcacg atgattttgg
     4321 tggcgccgct tacaagcttt tactctttaa ttacgccgcg agcgcgtaac agcgccgttt
     4381 tgaagtcttc ttcataatct ttctgcaaac ccgggatcac cgcatctttc gcggaatcac
     4441 gcattttcag gtgatagatc agaatgtcat ccgtcaaatc cgccagttcg ccgtcaaaac
     4501 ctgactcttt cgccagtttc tgcaaaaatt gcaacaagtt aaagtctggc tctttttgcc
     4561 aggctggctg gaggagttca ataacttcat tcagacgttt acatttcata gtaatgctcc
     4621 ttactcttga gacagacacg ttagcagggt caatcccaca ataaaagagg cgatatcggt
     4681 gaatctaatg acgacgataa caggcgttgt gctggcaggc ggtaaagcca gacgaatggg
     4741 cggcgtagat aaaggattgc tggacttaaa tggcaaaccg ctgtggcaac atgtcgctga
     4801 cgcgcttaag acgcagctct ctcacgtcgt ggttaatgct aatcgtcatc aggaaatcta
     4861 tcaggcaggc ggtctgaaag tgattgaaga ttcactggcg gattacccag gccctctggc
     4921 aggaatgctt tcagtaatgc agcaggaagc tggtgagtgg tttttgtttt gtccgtgcga
     4981 tacgccttac attccccatg atttagccgc ccggcttaat catcagcgca aagatgcgcc
     5041 tgtcgtgtgg gtccatgacg gtgaacgcga tcacccgact attgctctgg taaaccgcgc
     5101 tattgagcct ttattactgg aatatctgca agcaggagaa cgccgggtaa tggcatttat
     5161 gcgtctggct ggcggtcatg cggttgattt cagcgatcat aaagatgcat ttgttaacgt
     5221 gaatacgcca gaggagcttg cccgatggca ggaaaaacga tgataccgtt actcgccttt
     5281 gccgcgtgga gtggtaccgg aaaaactacg cttttgaaaa aactgatccc ggcattatgc
     5341 gccagaggga tccgtccggg gctgattaag catacgcacc atgatatgga tgttgataag
     5401 ccaggcaaag atagctatga gctgcgcaag gctggcgcgg tacaaaccat cgttgccagc
     5461 cagcaacgat gggccttgat gacggaaaca ccagacgaag aagagctgga tctacatttt
     5521 ctcgcaagtc gaatggatac ctcaaagctg gatttgattc tggtcgaagg gtttaagcat
     5581 gaagagatcg caaagattgt gctgtttcgc gatggagccg gacatcgacc tgaagaatta
     5641 gtgatagaca ggcatgttat tgctgtagcc agtgatgtgc cgcttaatct tgatgtcgca
     5701 ttactggata ttaatgatgt tgaggggctg gctgattttg tggtggagtg gatgcaaaag
     5761 cagaacggat aagcaagagt aggctggatc agacgc
//
LOCUS       DAZPEU010000209         5744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPEU010000209 DAZPEU010000000
VERSION     DAZPEU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.211"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..173
                     /locus_tag="ACHU2L_003608"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACHU2L_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..827)
                     /locus_tag="ACHU2L_003609"
     CDS             complement(489..827)
                     /locus_tag="ACHU2L_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358296.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            749..1876
                     /gene="ltrA"
                     /locus_tag="ACHU2L_003610"
     CDS             749..1876
                     /gene="ltrA"
                     /locus_tag="ACHU2L_003610"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1358297.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1959..2138
                     /locus_tag="ACHU2L_003611"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="ACHU2L_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2165..2650)
                     /locus_tag="ACHU2L_003612"
     CDS             complement(2165..2650)
                     /locus_tag="ACHU2L_003612"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1358298.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2906..3886)
                     /locus_tag="ACHU2L_003613"
     CDS             complement(2906..3886)
                     /locus_tag="ACHU2L_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR1358299.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="ACHU2L_003614"
     CDS             complement(3886..5085)
                     /locus_tag="ACHU2L_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1358300.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5259..5393)
                     /locus_tag="ACHU2L_003615"
                     /pseudo
     CDS             complement(5259..5393)
                     /locus_tag="ACHU2L_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5407..>5744)
                     /locus_tag="ACHU2L_003616"
     CDS             complement(5407..>5744)
                     /locus_tag="ACHU2L_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1358301.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAZPEU010000210         5741 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPEU010000210 DAZPEU010000000
VERSION     DAZPEU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.212"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            119..1027
                     /locus_tag="ACHU2L_003617"
     CDS             119..1027
                     /locus_tag="ACHU2L_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR1358302.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1073..3064)
                     /gene="tkt"
                     /locus_tag="ACHU2L_003618"
     CDS             complement(1073..3064)
                     /gene="tkt"
                     /locus_tag="ACHU2L_003618"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1358303.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3342..4100
                     /gene="loiP"
                     /locus_tag="ACHU2L_003619"
                     /gene_synonym="yggG"
     CDS             3342..4100
                     /gene="loiP"
                     /locus_tag="ACHU2L_003619"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR1358304.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4306..5226)
                     /gene="speB"
                     /locus_tag="ACHU2L_003620"
     CDS             complement(4306..5226)
                     /gene="speB"
                     /locus_tag="ACHU2L_003620"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR1358305.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5362..>5741)
                     /locus_tag="ACHU2L_003621"
     CDS             complement(5362..>5741)
                     /locus_tag="ACHU2L_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358306.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1377 a         1531 c         1515 g         1318 t
ORIGIN      
        1 ttttctaatg caatgttttt tcgctatacc cctttataga ttctgaattc tcacctgtcg
       61 gctgttttct cctcatctca tgtgacatat tcccatactt tactcaagga agattgtcat
      121 gaaagcacct gtttcattaa cctcatttcg cccacaaaaa tctcttgcta tcgccattgg
      181 cgtgcttgcc gtggttgtgc tgccatttct ctcgtattac accgttaatg aaggtgaacg
      241 cggcattttg ctgcgctacg gaaaaatcgt aaaagttgcc gaaccggggc tgggttttaa
      301 gatccctttt atggaaagcg tggagaaaat ttccacgcgt aatcaggctg tggtctatca
      361 gggattacag gcctacagcc gcgaccagca accggcacaa atgacagtat cagtcagctt
      421 ccacattaaa ccttcagaag caggcgcggt ttataccacc tacaacacta ttgaagcatt
      481 aaaagatcgt ctaattgtcc gtcagttgcc aactcagctg gagaacattt tcggccaata
      541 taccgccatc agcgccgtac aggatcgtac aaaattggtt caggatctgc aaaatgccat
      601 gcgcaaagcc gttgttgggc cagtggtaat tgacggggtc cagattgaaa atattgattt
      661 ttctgacgct tatgaaaagt cgattgaaaa ccgaatgaaa gcagaagtcg ccattgccac
      721 gcgtaagcaa aatctggaaa cagagaaaat ccaggcgcaa atagcggtaa ctcaggccca
      781 ggcagaagca gacagtaaac tggcggcagc caaagcagaa gcggaaacta ttcgcgtaag
      841 aggcgcggca gaggctgaaa ctattcgttt aaaaagtgcg gctgaagcag aagcaatccg
      901 cctgcgcggt gaagctctgc gggataatcc cggacttgta gccctcacca ctgccgaacg
      961 ttgggacggt aaactgcccg ataccatgat ccccggcagc acagttccgt ttatctctac
     1021 taagtaaaaa aagggccgct ttcgcggtcc ttctcacaaa acgtcttact gattacagca
     1081 gcgcttttgc tttcgcaaca acgttatcaa cagtgaagcc gaactcttca aacagcagct
     1141 ctgccggagc agattcaccg aaggtggtca tgccgacgat agcgccgttc aggccaacat
     1201 acttgtacca gtagtcagca atacccgctt ctaccgcaac gcgtgcagta accgctttcg
     1261 gcagtacgga ttcacggtaa gccgcatcct gcttgtcaaa tgcgtcggta gacggcatgg
     1321 acaccacgcg cgctttcacg ccttcggcag tcagtttttc gtaggcagca accgccagtt
     1381 caacttcaga accggtggcg atgaagatca gttccggctg accagcacag tctttcagca
     1441 cataaccacc gcgcgcgatg tttgccagct gctcttcagt acgttcctgc tgcgccaggt
     1501 tctgacggga gaggataagc gcagtcgggc cgtcctgacg ctcaacgccg tatttccacg
     1561 cgaccgcgga ttcaacctgg tcacacggac gccatgtaga catgttcggg gtcacgcgca
     1621 gagaagcgac ctgctctacc ggctggtgag tcgggccatc ttcgcccaga ccgatggagt
     1681 cgtgggtgta aaccatcacc tggcgctgtt tcatcagcgc agccatacgt acggcgttac
     1741 gtgcgtattc cacgaacatc aggaaggtgg aggtgtacgg caggaagcca ccgtgcaggg
     1801 agataccgtt agcaatcgcg gtcataccga actcgcgaac accgtagtgg atgtagttac
     1861 ctgcagcatc ttcgttgatt gctttagaac cagaccacag ggtcaggtta gacggtgcca
     1921 ggtcagcaga gccgccgagg aattcaggca acagcgggcc gaacgcttcg atagcattct
     1981 gcgacgcttt acggctggcg attttcgccg gattagcctg cagtttagcg ataaactctt
     2041 tcgctttggc gtcgaagtca gacggcattt cgcctttcat acggcgggta aattcagccg
     2101 cttcctgcgg ataagctttc gcgtaagccg caaacttctc attccatgca gattctttcg
     2161 cctggcctgc ttctttcgca tcccactgag catagatttc agacgggatt tcgaacggtg
     2221 catatttcca gcccagctgt tcgcgggtca gggcaatttc agcgtcgccc agcggcgcac
     2281 cgtgggagtc gtgagtaccg gctttgttcg gggaaccgaa accgatgatg gttttgcaca
     2341 tcagcaggga cggtttgtca gtcactgcgc gcgcttcttc aactgcgcgt ttgatggatg
     2401 ccgcgtcatg accgtcgatg tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg
     2461 ctgtgtcgtc ggtgaaccag ccttcaacgt gaccatcgat agaaataccg ttgtcatcgt
     2521 agaacgcaat cagtttaccc agcttcagcg tacccgccag agagcaaact tcgtgggaga
     2581 tgccttccat catgcagccg tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt
     2641 ggcccggacg gttaaactgc gccgccagcg ttttttctgc aatcgccata ccgactgcgt
     2701 tggcaatacc ctgacccagc ggaccggtgg tggtttccac accagcggta taacctactt
     2761 ccggatggcc cggagtttta gagtgcagct gacggaagtt tttcagttct tccatcggca
     2821 gatcgtaacc ggtgaggtgc agcaggctgt agatcagcat ggagccgtgg ccgttggaca
     2881 gcacgaagcg gtcacggtca gcccaggacg gattctgcgg gttgtgtttc aggaaatcac
     2941 gccacaggac ttcggcaatg tcagccatac ccataggggc acccgggtga ccggatttgg
     3001 ctttctgtac tgcgtccatg ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg
     3061 acattttgac tccagatcgg atgatgaagg gcacgccctt aacgacttga cgacagcgcg
     3121 ttttgggcta cgccggaaaa tttgccaaca atttaccgca agccgcgcgt catgtacatg
     3181 gaacatcctt ttgccgcttc agaaatctct ggatcatgct cgcatgttgc gcaatctgct
     3241 cgcccgtccg ctgcgctttt ccttatactg agactgagcg tcgattcacc tgcaaacggc
     3301 gcatttttag aataatcctg accttgtgcg gaagagaaaa catgaaaatt cgcgccttat
     3361 tggtagcaat gagcgtggca acggtactga ccggttgcca gaatatggac tccaacggac
     3421 tgctctcatc aggagcggaa gcttttcagg cttacagttt gagtgatgcg caggtgaaaa
     3481 ccctgagcga tcaggcatgt caggagatgg acagcaaggc gacgattgcg ccagccaata
     3541 gcgaatacgc taaacgtctg acaactattg ccaatgcgct aggcaacaat atcaacggtc
     3601 agccggtgaa ttacaaagtg tatatggcga aggatgtgaa cgcctttgca atggctaacg
     3661 gctgtatccg cgtctatagc gggctgatgg atatgatgac agataacgaa gtcgaagcgg
     3721 tgatcggtca cgagatgggg cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg
     3781 cgcttggtac aaatgccgtg cgagtagctg cggcctctgc gggcgggatt gtcggcagtt
     3841 tatctcaatc acaacttggt gatctgggcg agaaattagt caattcgcaa ttctcccagc
     3901 gccaggaagc agaagccgat gattattctt acgatcttct gcaccaacgc ggcatcagcc
     3961 cggcaggtct tgccaccagc tttgaaaaac tggcgaagct ggaagaaggt cgtcaaagct
     4021 caatgtttga cgaccatcct gcatccgccg aacgtgccca gcatattcgc gatcgcatca
     4081 gcgcggatgg gattaagtaa agcctagtgg tgtcgaacgt aggtcagata aggcgttcac
     4141 gccgcatctg acattaatgg cacatttgtt accttgtgcg cattgccgga tgcgatgctg
     4201 gcgcatctta tccggcctac gggtgccgaa cgtaggtcgg ataaggcgtt cacgccgcat
     4261 ccgacattaa tggcacgttt tacccgtgcg catcgcatct ggtgcttact cgcccttttt
     4321 cgccgcctga atatacagca tctccagcgc cagcgtcgcc gcagccagag cggtgatttc
     4381 cgactgatcg tatgccggag ccacttccac tacgtccatc ccaacgatgt tgagatcttt
     4441 caggccgcgt accagtttaa tggcgcgatc ggaggtcagg ccgccaatca ctggcgtacc
     4501 ggttcccggc gcgaaagcag gatccaggca gtcgatatca aaagtcaggt aaaccggcat
     4561 atcacccaca atctgtttca cctgggcgat aacgtcatcc acgctgcgat cgttcacctg
     4621 gcaggcgtcc agcacggtaa agccgttgtc tttatcaaac tcggtacgaa taccaatctg
     4681 cacggaatga ttcgggtcga tcagaccttc tttcggcgcg gtatagaaca tagtgccgtg
     4741 gtcaaattca caaccgttcg cataggtatc ggtgtgggcg tcaaagtgta ccagcgccat
     4801 tttgccgaaa tgcttcgcat gagcacgcag cagcggcagc gtaacaaagt ggtcaccacc
     4861 gaaggagagc atacgcttac cggcagccag cagcttctcg gcgtgcgcct gcagcttttc
     4921 gctcatctca cgggcatcgc caaaggcata taccagatcg ccgcagtcca cgacgttcag
     4981 acgctcacgc atgtcgaaat tccacgggaa gcggttgtgt tcccaggcca gattcgtcga
     5041 aacctgacgg atcgctgccg gaccgtggcg accacccgca cgaccagaag tggccatatc
     5101 gaacggcacg ccagtaatca cccagtctgc atcgctgtca tacggctgga agttcatcgg
     5161 caggcgtaaa aaaccaaagg cattggaaac cagtgagtta tcgtattgat gacctaaggt
     5221 gctcatggat ggactcctat tacaaagtcg atataaaaaa acccttccgc gtcgttaggc
     5281 ccgacgagga agggttggat ttgcgaaaca caagtttgtg ctaattatcg ccttaaatgg
     5341 gcgaggcatc aagcgaggtt gctatttccg acacggtggc ggcgcatagc cattgttatc
     5401 tgccggaaag gcttgtccag agcacggcgg cagcgcgttt tgcgccgggc cttgtaattc
     5461 aaaggcggca aagccgatta ccccggtatt acgcacatcg ccttgcgccg aattggctgc
     5521 gtaggaatca gcaacgttgg cgaactgaaa agccgcttca gtatgtttat ctttgcggaa
     5581 tcctttaatc actaacgaat ctccggcatt cacaatatag ccgttgttat tcagcgaacc
     5641 ttgcttgccg ttcagcacat ccaggccatc agccgtagca acgatctcgt agttggtgtt
     5701 acgactgtaa ttacggacat ataattgata attcattccg a
//
LOCUS       DAZPEU010000211         5738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPEU010000211 DAZPEU010000000
VERSION     DAZPEU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.213"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..241
                     /locus_tag="ACHU2L_003622"
     CDS             <1..241
                     /locus_tag="ACHU2L_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358307.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHU2L_003623"
     CDS             complement(238..1179)
                     /locus_tag="ACHU2L_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR1358308.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHU2L_003624"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHU2L_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHU2L_003625"
     CDS             complement(1684..2904)
                     /locus_tag="ACHU2L_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR1358309.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHU2L_003626"
     CDS             complement(2908..3651)
                     /locus_tag="ACHU2L_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1358310.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHU2L_003627"
     CDS             complement(3542..5008)
                     /locus_tag="ACHU2L_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR1358311.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACHU2L_003628"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACHU2L_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAZPEU010000212         5726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPEU010000212 DAZPEU010000000
VERSION     DAZPEU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.214"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            3..800
                     /gene="gadX"
                     /locus_tag="ACHU2L_003629"
     CDS             3..800
                     /gene="gadX"
                     /locus_tag="ACHU2L_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR1358312.1"
                     /translation="MAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVL
                     VSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEM
                     LRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELL
                     MSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYV
                     FRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1168..1896
                     /gene="gadW"
                     /locus_tag="ACHU2L_003630"
     CDS             1168..1896
                     /gene="gadW"
                     /locus_tag="ACHU2L_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR1358313.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2259..5372)
                     /gene="mdtF"
                     /locus_tag="ACHU2L_003631"
                     /pseudo
     CDS             complement(2259..5372)
                     /gene="mdtF"
                     /locus_tag="ACHU2L_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5397..5654)
                     /locus_tag="ACHU2L_003632"
                     /pseudo
     CDS             complement(5397..5654)
                     /locus_tag="ACHU2L_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1489 a         1371 c         1386 g         1480 t
ORIGIN      
        1 taattgcgta cgcaagacat aaatatattc tcaccatggt taatggtgaa tatcgctatt
       61 ttaatggcgg tgacctggtt tttgcggatg caagccaaat tcgagtagat aagtgtgttg
      121 aaaattttgt attagtatca agggatacgc tttcattatt tctgccgatg ctcaaggagg
      181 aggcattaaa tcttcatgca cataaaaaaa tttcttcatt actcgttcat cactgtagca
      241 gagatattcc tgtttttcag gaagttgcgc aactatcgca gaataagaat cttcgctatg
      301 cagaaatgct acgtaaaaga gcattaatct ttgcgttgtt atctgttttt cttgaggatg
      361 agcactttat accgctgctt ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta
      421 cggttatcaa taataatatc gcccatgagt ggacactagc ccgaatcgcc agcgagctgt
      481 tgatgagtcc aagcctgtta aagaaaaaat tgcgcgaaga agagacatca tattcacagt
      541 tgcttactga gtgtagaatg caacgtgctt tgcaacttat tgttatacat ggcttttcaa
      601 ttaagcgagt cgcagtgtcc tgtggatatc acagcgtgtc gtatttcatt tacgtctttc
      661 gaaattatta tgggatgacg cccacagagt atcaggagcg atcggcgcag ggattgccga
      721 accgtgactc ggcggcaagt attgttgcgc aagggaattt ttacggcact aaccgttctg
      781 cggaaggaat aagattatag agttttactc agacataaaa aaaacccggc ataggggacc
      841 gggaagagga tagtctgccg tctccagact aataaaccgt tataacactc cctgttggca
      901 cgggaaactt tgtgctctca gtaagttaaa tataactttt acaggaaata agatcagcca
      961 tttttttata aacataagct atacgctgtg cgaaaatata ttcgtgctgc atttacttat
     1021 tatcaattaa ctgttatgca aaactacttt gtggataaat tttggtccta ccaaatctgg
     1081 cagtttttgc gctaagaaac agtctgtcat catttcatta gtatactgaa attgaaataa
     1141 tcgcagtatg aaatataagg gataatcatg actcatgtct gctcggtgat cctcattcgt
     1201 cgttcattcg atatttatca tgaacagcaa aaaatatcgc tgcataacga gagtatcctg
     1261 ctgctggaga aaaatttggc agacgatttt gcgttttgtt caccggatac gcgacgactg
     1321 gatatcgatg agctgacagt ttgccattac ttacaaaata ttcgtcagct accacgcaat
     1381 ttagggttac atagcaaaga ccgtctgtta attaaccagt caccccccat gccgctggtg
     1441 acggcgattt ttgatagctt caatgaatcc ggggtaaatt caccgatact gagcaatatg
     1501 ctctaccttt cctgtttatc gatgttttct cataagaaag aactgatccc cttacttttc
     1561 aatagcatca gcactgtttc aggaaaagtt gaacgcctta ttagctttga tatcgccaaa
     1621 cgttggtatc tgcgcgatat cgcggaaaga atgtatacca gcgagagtct catcaaaaaa
     1681 aagttgcagg atgaaaatac ctgtttcagt aaaatattac tcgcttccag gatgtcgatg
     1741 gccagacgat tactcgagtt acgtcaaatt cctctgcata ctattgcgga aaaatgtggc
     1801 tatagcagta cgtcgtactt tataaacaca tttcgacaat attatggtgt aacgccacat
     1861 cagtttgcgc aacattcgcc aggtaccttt tcctgacata ttttgcattt gaatattggt
     1921 caggatctca cacctgcttc atgtgaaact cttccctgat gatttctgcc gggctaccgg
     1981 ctagttctct ttcgcagttt gccaggcggt ataggtattt ttaggaggaa aagactcggc
     2041 atgtttggga ttattaagct gacaattcat acgattacgg gaatgtgggt cagtattgtg
     2101 ttgttcaaat tgatgacgaa tggctggagt ggtttctact ttcagtgctg tgtactaagc
     2161 ctggtattcc tgacggttag ctggttgttg agtggtgaat ggttagcagg aaagagtaag
     2221 gctgaacctt cacgttcaac cttactctca tttacacgtt acgctttttt aaagcgggca
     2281 aagagatgtt ccactacaac gaaaaagacc ggaacgaagt aaattgccag cactgttgcg
     2341 gcaaacatcc cgcccatcac gccggtacct accgcgtttt gcgcgccaga accggcacca
     2401 tgactgataa ccagcggcag cacgccgaga ataaaggcca gagaggtcat caggattggg
     2461 cgtaaacgca tccgcgccgc ttcgatgatt gcctctatcg gcgttttccc ttctttctgc
     2521 atcatctcaa cggcaaattc gacaatcagg atggcgttct tggcggaaag cccgatggtg
     2581 gtcagcaaac caacctggaa gtagacgtca ttacttaagc cgcgcagatc ggtggccagt
     2641 aatgcgccaa cgacgcctaa cggaacaacc aacatcaccg agaacggaat tgaccagctc
     2701 tcatagagtg cggcgagggc gaggaacacc acgaccagtg aaatggcata cagtgcagga
     2761 gcctggtttg aggataacgc ttcctgatac gacagtccgg tccatgagta gccgacgcct
     2821 gccggaagtt tagcgaccag gtctgccata aatttcatag cgtcaccggt acttttcccg
     2881 gccgccgctt cacctaaaat ctccattgac gggatgccgt tgtagcgttc caatcgcggt
     2941 gaaccatagg tccattcggt agacgagtag gcagaaagcg gtgccatcgt gccagaggcg
     3001 ttgcgtacat accattggtt gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg
     3061 acatacactt ttttcacccg cccctggttg aggaagtcgt tcacgtagct gctgccgaag
     3121 gcggtggaaa ttgtctggtt gatatcagac agcgccacac ccattgcctc agctttcgcg
     3181 gcgttgacgt tcactttgaa catcggcgta tcttccaggc cgttcgggcg taccccggtg
     3241 acctggtcag gcgattgcgc tgccagtgat aacagctcgt ttcgtgccta ggttagtttt
     3301 tcgtgcccca ggttaccgtt gtccagcagt tccatatcaa aacctgacgc ggtacccagt
     3361 tcagccaccg cgggtaagtt gaacgggaag acgacggctt tattgatact gcttaacgca
     3421 atcattgccc gctgaatgat cgcggtaacc gagttttcct caccgacacg ttcagaccac
     3481 ggcttgagac tgataaacgc caggccgttg ttttgcccct ggccgctgaa gccaaagccg
     3541 ccaacggtaa acaccgactg aacattatct ttctcttttg taagataata atccgtcacc
     3601 tgttgcagta ctttcgtggt gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc
     3661 ataaataccc cctgatcctc ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc
     3721 gccatcccgg cgcaaatcag caggtagacc accatgtagc gaccggtaca acgcaacagc
     3781 gagcgggtgc tatcggtata gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg
     3841 aacagggcgt taggtttgtg accgccttcc ggcgtggctt tcagaatggt ggcgcacagg
     3901 gcaggggtca ggctcattgc cacaaatact gaaagccgca tggaggagat cagcgtgatg
     3961 gagaactggc ggtagatctc cccggttgca ccgctcataa aggccatcgg cataaacact
     4021 gcagaaagaa caacggcaat cccgaccagc gcacgttgga tctgccccat cgatttatgc
     4081 gtcgcttcct tcggcggtag cttatcttcc gcaatgacac gctcgacgtt ctccaccacc
     4141 acgatggcgt catccaccag taaccctatc gccagcacca tcccgaacat agtcaacgtg
     4201 ttgatggtga aaccgaccgc cgacaagatc gcaaacgtcc cgagaataac caccggtacg
     4261 gcaattgtcg ggatgatggt ggcacggaaa ttctgcaaaa acagataaat gaccaggaag
     4321 acgaggatga tagcctcaac cagtgttttg aaaacttcct gaatagaaat ttcgataaac
     4381 ggcgtggtgt cgtaaggata aaccgtcttc agacttgcgg ggaaataggc tgataagcgg
     4441 ttcagctcct ctttgactgc ccgcgaggta tccagggcgt ttgctccggc agccagtttg
     4501 atggcgatcc cggcaacagg tttgccgtta tagcgcgcca cggtggaata atcttccgcc
     4561 ccaagttcga cgcgggcgac atcacgcagc agcacttgcg aaccatcttg ctgaactttc
     4621 aacaggattt tgccaaattc ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg
     4681 tttagttgct ggtctgccgc ctgtggcatg ccacccagtt gaccaccaga aatctggttg
     4741 ttttgcacct taatctggga aataacatcg gaaggtacca ggttatattt attgagtttt
     4801 tgcgggtcca gccagatacg catggcgtac tcggaaccaa agagctgtac gctaccaacg
     4861 cccgcggtac ggcttagcgg gtctttgata ttagacgcta catagtccgc gatatcgtac
     4921 tggttgaggc tgccgttatc agaaataaac gccgctacca tcaggatatt actgctcgac
     4981 ttatcgacgc taatcccctg ttgctgcacc gcttcaggta atgaaggcat agcgagttgc
     5041 agtttatttt gcacctgaac ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga
     5101 gtgatagagg cattacccgc cgcatcactg gttgaagaca tgtacatcag gccatcaagc
     5161 ccattcatat tttgctcaat cacctgagtg accgagtctt ctaccgtttg cgcatcggca
     5221 cccggatagg tagcgctgac ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc
     5281 ggtaagttca tgatcgccag accacctgca agcatcataa taatggcaag tacccaggca
     5341 aaaaccgggc gatcaataaa atagttagcc atgaaagtcc ccttaagcct gcaacgttat
     5401 tgtttcgatt cggtgctggc gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc
     5461 ggacgaatgc gttgcaaacc ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg
     5521 acaacccact ggtcgccgat ggctttgctg gcttcaattt cgcgtagctg cacgacatcg
     5581 tctttatcca gaatgagcgc cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc
     5641 ggcaccagta atataaagcg cgcaaaatta ttcaagatat ttttgaaaat cattcaagat
     5701 aggctatcgc gtgtgatagc aaaaac
//
LOCUS       DAZPEU010000213         5702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPEU010000213 DAZPEU010000000
VERSION     DAZPEU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.215"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHU2L_003633"
     CDS             complement(<1..170)
                     /locus_tag="ACHU2L_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358314.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHU2L_003634"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHU2L_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR1358315.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHU2L_003635"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHU2L_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHU2L_003636"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHU2L_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR1358316.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHU2L_003637"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHU2L_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHU2L_003638"
     CDS             5484..>5702
                     /locus_tag="ACHU2L_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR1358317.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZPEU010000214         5684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPEU010000214 DAZPEU010000000
VERSION     DAZPEU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.216"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACHU2L_003639"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACHU2L_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR1358318.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACHU2L_003640"
     CDS             2739..4208
                     /locus_tag="ACHU2L_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR1358319.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>5684
                     /locus_tag="ACHU2L_003641"
     CDS             4208..>5684
                     /locus_tag="ACHU2L_003641"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR1358320.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMP"
BASE COUNT         1687 a         1335 c         1338 g         1324 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaa
//
LOCUS       DAZPEU010000215         5675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPEU010000215 DAZPEU010000000
VERSION     DAZPEU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.217"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(105..1817)
                     /locus_tag="ACHU2L_003642"
     CDS             complement(105..1817)
                     /locus_tag="ACHU2L_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HFR1358321.1"
                     /translation="MPEQLYNVVVSQPSQLFTLARSFKANANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQQFY
                     FPNLLKYDPDQLKQLLQSDEGATYIGTNSGKNLQDELFAIKNEIYQDPLTSWPFGGTL
                     KIKQGVYDVTTPLVLNYDDYPADFVGAPGVRCFYSGENMAETILECRATDFNVKMLGD
                     AAFNSQRLAAYDYIGNMTLKGNPGSYGMLVQNKAHTRLENIVICRHPDGEGLRTDSML
                     TSDMTNVYLQSNRIGWRATNSVNHSELNAITANRLTCSQNSEWGILGDRWGAGTTINS
                     LTCEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADLAIDNTGTRPVTV
                     VINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGA
                     NCEVVDIGCVFTETTSKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVS
                     FSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSI
                     TRLL"
     gene            1882..2340
                     /locus_tag="ACHU2L_003643"
     CDS             1882..2340
                     /locus_tag="ACHU2L_003643"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1358322.1"
                     /translation="MEVLYYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            2472..2732
                     /locus_tag="ACHU2L_003644"
     CDS             2472..2732
                     /locus_tag="ACHU2L_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFR1358323.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(2822..4819)
                     /locus_tag="ACHU2L_003645"
     CDS             complement(2822..4819)
                     /locus_tag="ACHU2L_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1358324.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTHGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPEGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRVQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(4819..>5675)
                     /locus_tag="ACHU2L_003646"
     CDS             complement(4819..>5675)
                     /locus_tag="ACHU2L_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR1358325.1"
                     /translation="ELTRVGVDPNNVAQMYQQNPSGFGEFVDHLGMAALGPIDYFNVQ
                     DKMAGREIDRGRLAETIRSNQAGEALTARGQDIQIRGQNISAQNAALSREIQRAELQE
                     KALDRQIARESNQLKLEELKQKQADVRQKAEIARADRQAAAQGAVDTFSTALDSLNEI
                     EQSPGLSKAVGIRSAFPTVPGSDAANFEARLDTFKAQTFLPMVQSLKGMGALSDAEGK
                     KLSDAVGALSPKMSEKAFRDSIGKIRNQLESKLSTVKKQFDYQEPVQNTPGQQSPAGS
                     NFSSLWGD"
BASE COUNT         1444 a         1390 c         1267 g         1574 t
ORIGIN      
        1 tccctccatg gttaactctt gatttaataa aaaaataaaa cttcacatta ataaataaca
       61 atgaatccgc ctccctacgt attcccgtag ggaggctgtc gtgtttatag tagcctggta
      121 atgctaaatg caaacgaact agagattcct gctccgctaa gcgtattgcg cagggtcacc
      181 ctaaatgtat ctacgccaat ataagtaaca tcacaactaa ctgaatcagg cgcagatata
      241 ggtgttatct gcacaatata accgctagca actgctgcga gcggatgaga aaagctaacc
      301 tcatacacac ctgtagcaac cacgttaacc gatgaaacac cagtagcaac gtcgatacta
      361 ccattggcgt taatttttcc cgagcgtgtg actgacaatg cagaagccga tgttgcctta
      421 ctggttgttt ctgtaaaaac acacccaatg tcaactacct cgcaattagc tcctgttatc
      481 cagtatggtc gatctgcact tggctgataa tcccctgcgc tctggaacgt tgtgccgttg
      541 agtatgactg taacttttcc cccacctgag cttgttactt gaatattagt gtgcgtgtat
      601 cttgcactgc tcacgcgatg aaaatttcca ccgtttataa ccacagttac agggcgcgtc
      661 ccagtattat ctatagcaag gtctgccccc ccggcattag cctcaaagta tgggttgttt
      721 aataccaaag cgcacgaacc gttaaggcca tttatggcta attgcgcacc acctgtactg
      781 gaatccccct gagtaccatt tccttcacaa gtcaggctat tgatagttgt accagcaccc
      841 cagcggtccc cgaggatacc ccattcactg ttctgactgc acgtaagcct atttgcagtt
      901 atagcgttca gttcagaatg atttacggag ttggtagcac gccaaccgat acgattactc
      961 tgcaagtaca cgttcgtcat gtcgctcgtg agcatcgagt cagtgcgtaa cccttccccg
     1021 tcagggtggc ggcagataac aatgttttca agacgagtat gtgctttatt ctgcaccagc
     1081 ataccgtaac ttccgggatt ccctttcagc gtcatgttgc cgatgtagtc atatgcagcc
     1141 aatctctgac tgttaaacgc tgcgtcacct agcattttta cgttaaaatc agtagctcta
     1201 cattccagta ttgtctcagc catgttctcg cctgagtaaa aacagcggac acccggagca
     1261 cctacaaagt cggcagggta atcatcataa ttaagtacca gaggagtggt aacatcatac
     1321 accccttgct ttatttttaa tgttccaccg aaaggccacg atgtaagcgg gtcttgatat
     1381 atttcatttt ttattgcaaa tagctcatct tgaagatttt tcccgctatt agtgccaatg
     1441 taagtagccc cttcatcaga ttgaagtaac tgctttaact gatccgggtc atacttcagc
     1501 aaattcggaa aatagaactg ttgcgaacca tacgcatcat atacagccat tgaatggcct
     1561 tgctcagtta caaatttggc aatctgcccg ttgtacacag gatatccggc agcattgata
     1621 acaattggct gagaaacagg gacgtgagat ccgtcttcat tctctacata aacctgaatc
     1681 tggttttcag gatttaccgg gtcagtgtca attttcccga tataaatttt tccattggca
     1741 tttgctttaa acgagcgagc taaagtaaat aactgacttg gttgtgaaac aacaacgttg
     1801 tataattgtt ctggcataat aaacattccg gagaaaataa tgagtaagag acgtcacgag
     1861 gtagtaactc gggagcgcct tatggaggtt ctctattaca ataaagaaac aggaattttc
     1921 acttggaaaa agaaattaag cgccagaggt gctgttggga aagttgctgg cacaatttct
     1981 tatggatata acgcaattaa tattgatggc gtaagatatt ttgcacatag acttgcctgg
     2041 ctgtatgtat atggcgaatg gcccaaacaa gagattgacc acattgatag aaacagaaga
     2101 aataacgcaa tctcaaacct tagggatgtc agcagagtcg taaatgcatt aaatgtcgga
     2161 cctcgaaata gcaatgctgg aatcaagggg gtaactttct gtcaagccag aaatcaatgg
     2221 caagctcaga taaatgtctc aggtaaaaac ataacccttg ggagattcaa caccattgat
     2281 gaagcggcta tagcttataa ggcagcaaac atggttgctg accatctgtt gagtaggtga
     2341 tatctgacat ttcattgctc cagacgaatg atatgatgca accatgatgt gattgcatac
     2401 cgaaatggta ctattgagta tttatccagt aagttacgat gccattccac ccaattggtg
     2461 aggcatcaag gatgtacagc aaatacgacg aggcgcagtt tcacttgaga cttccgcatg
     2521 aactccacgc gaaaattaag cagcgtgcga agatgaataa caggtctctg aactcagaga
     2581 taattgcagc gattgaagaa tcactggata aacaaaactc tgcatcagtt tacattgacg
     2641 atgcagagcg tatggcagaa caacaatccg atatggttaa gaaaattgtc tttgatacgc
     2701 tcaaagagct atataaaaaa gacagcagct aaccatcagt tacggaggat ttatgcaaag
     2761 agatatgctg aatattgcgt tctacatatt tggtttttgc acgttcctgg tgtttgcgaa
     2821 gctattctga caacgcatca gacttggcac cctgagtcag ggcgttaatg gccttttgcg
     2881 cctgctgcat ggctttctca aacgctgttg atccgcgtgg ggtatttgcc attcggagca
     2941 ttgcatttct gaatggctcg ctctcatagg cgcgagtaag aagtccgtag cttaccgctg
     3001 cgccagttgt cgccgggttc attgccgtcc catacccgat aatgaacggg atggtttgct
     3061 gccctgttgg tgttgttact gccgcttttg ctgcctgctg agtggactga aggtaatttt
     3121 tcaatccttt cagataagca gcgtcctgcc ccttaaatgt gatgccagtc tggttttgca
     3181 ggatgttaag ctgccgaagg aactggtcag gggatccgcc agatttctcc atcgcctttc
     3241 caatgatgcc attgcgcatt tgcaccctgc caacacgacc aactgagtta tacagcgtct
     3301 taatttccga tttgttcttg ctgaatagca tgttgttgac aacttccggc gtcaggtcgc
     3361 ctttcatgag aacattcttc agcctggtat tctttagttt cgccgcttcg tcagcgtaga
     3421 cggcattggc ctgctgatat ttacggagag tatcgttgcc aagattctga ccaatggcac
     3481 cattgatatc gtcggtcatc gccttgtaaa cgcgctgaat ggcagcatcg gaacggtttg
     3541 gtaacactgg tcgctcaccc ttcacgtcca ttctgaactg actgcgcagg tcgcttaatt
     3601 gcttcaaatc cagatttacc ggaccttcag ggccagcatt gcgaacaagc tcatcacgat
     3661 atgactgaag ttttgaaatt gtctcgttat cagccacctt accaagcttc tgcaggttag
     3721 atatttctgt atcaatctgc tgaattgctc gcgcaggctg aatgtttact ccagccatag
     3781 cattctgaac ctgctccagt cgattaccgg cggcacgacg aattcctgat gttttcgctt
     3841 taaggctgtc aataacaacc gctggatcat actccccgaa tttatcagca aatctctgca
     3901 ccaactggct tctcgcttcc tgttgcgttg ctctcattcc gcttgtgcca gccagaggga
     3961 tattttctgc tgtagtctgc gccatttttc cgacgcggga agttggttgt aacaggtctg
     4021 tggtgtgcag aggaactcct tcacgctctg caaatctgat agcctgctga gcttctggcg
     4081 cgatagcacc acgaacgcca cgataagcag cacctaatcc acgtccggca gcgttaatag
     4141 caccgccagc aagtacacca acgcctaaat cggtggcgag tgcttccgca tcatctttcg
     4201 cactatttgc agcaagtgat ccaactgcgt tttcagcgag aaggcgagtt gcaccctgag
     4261 caattcgacc agcaagtgtt ggtgcctgtg ttgccgctct ctcaacgccg gcaggagtga
     4321 ggtaaggcaa tgcttcagca aataccctgc cctctgtcgt ttgtggagtc agcgcacctt
     4381 gctgaaggcc aaagtcctgc tctaatccat gcgttgttac tcgtggcgct ggttgatatg
     4441 ttccatcgcc aatgccgagt ttaccgccag cccatgcagc cgcgcttgtt acagcatcgg
     4501 caaccgatgc aggtatgttt gccacgttca cgccagcctg caccagtccg cgaccagtct
     4561 cttttactgc ttcgccaaga tcagacataa atccactttg ctgtggttgt tgctgtgcta
     4621 ctggttgctg tgtctccact ggctgcacag atggcaatgg ataggcagca tagaaagctt
     4681 gcttagcctg ctctgcattt tctccggctt gcggggcaac gacttcattg aagtattgct
     4741 cctgagcctg cgctttttgt tctggtgcta acgcctgata ctgtggagat gcgataacat
     4801 ctttccatgc tttagccatt aatcacccca tagtgaagaa aaattactgc cagcaggaga
     4861 ttgttgccct ggcgtattct gcaccggctc ctggtaatca aactgttttt taacagtgct
     4921 caacttgctt tcaagctgat ttcgaatctt tccgatagag tcacgaaaag ccttttcact
     4981 cattttggga cttagagcac caaccgcatc ggataatttt ttaccctcag catctgaaag
     5041 agcccccatc cccttcaggg actgcaccat agggaggaaa gtttgagctt taaaggtgtc
     5101 gagtcttgct tcaaagttag ccgcatcaga tccaggaact gtcggaaacg ctgagcgaat
     5161 accaactgct tttgaaagtc cagggctttg ctctatctcg ttgagagaat caagcgcagt
     5221 gctgaacgta tcaacagcgc cctgagcggc ggcctgtctg tcagcacgag ctatttcagc
     5281 cttttgccga acatctgcct gtttctgctt tagctcttca agctttaact gattgctttc
     5341 tctggctatc tgtctgtcca gagccttttc ttgtaattct gctctttgta tttcgcggga
     5401 aagagcagca ttctgtgcgc tgatgttctg gccacgtatc tggatgtcct gacctcgtgc
     5461 tgttagtgcc tcgccagcct gattgctgcg gattgtctct gccagcctac ctcggtcaat
     5521 ctcacgacca gccatcttgt cctgaacatt gaagtaatca atcggaccaa gagcagccat
     5581 cccaaggtga tcaacaaact caccaaatcc tgaaggattc tgctgataca tctgagcaac
     5641 gttgttagga tcaacgccga cgcgagtcag ttcct
//
LOCUS       DAZPEU010000216         5674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPEU010000216 DAZPEU010000000
VERSION     DAZPEU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.218"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(294..1253)
                     /gene="nrdF"
                     /locus_tag="ACHU2L_003647"
     CDS             complement(294..1253)
                     /gene="nrdF"
                     /locus_tag="ACHU2L_003647"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1358326.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1263..3407)
                     /gene="nrdE"
                     /locus_tag="ACHU2L_003648"
     CDS             complement(1263..3407)
                     /gene="nrdE"
                     /locus_tag="ACHU2L_003648"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1358327.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3380..3790)
                     /gene="nrdI"
                     /locus_tag="ACHU2L_003649"
     CDS             complement(3380..3790)
                     /gene="nrdI"
                     /locus_tag="ACHU2L_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR1358328.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3787..4032)
                     /gene="nrdH"
                     /locus_tag="ACHU2L_003650"
     CDS             complement(3787..4032)
                     /gene="nrdH"
                     /locus_tag="ACHU2L_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR1358329.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4280..4609)
                     /locus_tag="ACHU2L_003651"
                     /pseudo
     CDS             complement(4280..4609)
                     /locus_tag="ACHU2L_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            4761..5105
                     /locus_tag="ACHU2L_003652"
     CDS             4761..5105
                     /locus_tag="ACHU2L_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR1358330.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5142..5591)
                     /gene="alaE"
                     /locus_tag="ACHU2L_003653"
     CDS             complement(5142..5591)
                     /gene="alaE"
                     /locus_tag="ACHU2L_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR1358331.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1447 a         1408 c         1416 g         1403 t
ORIGIN      
        1 ttttctaccc taacatactg agaatctgag gcaacccctg agggcaaatg tggcatgaat
       61 attgatgcga gcaaatcgcc ggaattttcc gtgatataag ggctgagagc aaatcgagaa
      121 aagttgatat tttttgtcat agatagcgaa aaaacgcctg aatattgtag tttcaggcga
      181 ataatgcaac agagtatcag tgtagatcac tacagaatat ttgcgtgata aaaagctatt
      241 tggcgggaat tatttccctg ctgcgggtag tgatattttt gaaaataaca ccctcagaaa
      301 ttccagtctt catcttctgt ttcaaccgct ttccccatca cataagagga gcctgaaccg
      361 gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg cggcgaggat tgccggattc
      421 acttccgcca tttctgcggg aaataacggt tcgtagccca gattcatcaa agccttattg
      481 gtgttgtaac agagaaacgc tttcacatcg tcagcccacg gggtttcggc gtacagctca
      541 tcggtgtatt gcaactcgtt gtcgtagagt tccagcaaca aatcgaaggc gaaactcttc
      601 aactcttcac gttgtcccag agatatcttt tccatgtttt tctgatattt atagcctatg
      661 tagtaaccgt ggactgcttc atcgcggata atcagacgga tcaggtccgc ggtattggtc
      721 agctttccgc ggctggaaaa atacatcggc agccagaaac cggaatagaa caaaaaagat
      781 tcaagaaaca cactggcgat tttctttttc agcggatcat caccgcgata atgttgctga
      841 ataatctgag cttttcgctg caacggtgcg ttttcttcac tccaggcgta ggcggcatcg
      901 acatctttgg tctggcacag cgtcgagaaa atcgaactgt aagagcgggc atgaaccgct
      961 tccataaagc tgatattcga taataccgcc tcttcatgag gcgtgagcgc atcgggcatc
     1021 agcgaaggcg cgccgataac attttgcagc gtgtcgagca gcgtcaggcc agtaaaaacg
     1081 cgcatcgtca gttgttgttc tacgacagtt aatgtctgcc aggcgggaat atcgttcgac
     1141 agcggcacct tttctggtag ccagaaattg ctggtcaggc gattccacac ctccagatct
     1201 ttatcgtcag atatcttgtt ccagttgatg gcgctgatac gtgagagttt catagatatt
     1261 ccttaaagtg cacaggagac gcagccttca atttcagtgc cttccagcgc catctgacgc
     1321 aggcggatgt aatagagcgt tttgatacct ttgcgccagg cgtaaatctg cgctttgttg
     1381 atatcgcgag tggtggcggt atcggggaaa aacagcgtca gcgacagccc ctgatcgaca
     1441 tggcgagtcg cttccgcgta ggtgtcgatg atcttttctg cgccaatttc gtaagcgtcc
     1501 tgatacagcg ccagattttc gttagtcata aacggggcag ggtagtaaac gcgtcctgtt
     1561 ttgccctctt tgcgtatctc tactttcgcc acaatcggat gaatactcga cgtagcatgg
     1621 ttgatataag agatagaccc ggttggcggc accgcctgga gattctggtt gtatatgccg
     1681 tagcgcatca cgtcgtcgcg cagttgcgcc cacatctcac gggtaggtaa cgtaatacag
     1741 ctgcgggcaa acagttcgcc aactttcgcc gttttcggct gccagttacc ttgcagatat
     1801 tggctgaaat attcaccact ggcatagcgt gactgtttga acccggcgaa ggtttcaccg
     1861 cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc aggtgatggt atagaaatag
     1921 agattggtga aatccagggc ttccggcgaa ccataagcga tgccttccct cgccagataa
     1981 ccatgtaaat tcatctgccc cagtccgatg gcgtgcgagg cggcatttcc ggcttcgatg
     2041 gacggcacgc tgcggatatg actcatatct gacaccgccg ttaaaccgcg cacggcagtc
     2101 tctaccgtgc gggaaaaatc gggggaatcc atggtgtgcg caatattcaa cgaacctaaa
     2161 ttgcaggaaa tatcatggcc tgtgcgggca tagtcgagat tctcgtcata ctctgaggcg
     2221 ctgttaacct gcaaaatttc tgagcagaga ttactcatat ttatgcgccc ggcgataggg
     2281 ttagcacggt ttactgtgtc ttcatacatg atgtagggat agccggactc aaactggatt
     2341 tctgccagtc gctggaagaa atcacgggcg ttgaggtatt ttttgcggat gcgttcatcg
     2401 gcaaccagtt cgtcatagtg ttcgctaatg gcgatatcgg caaacggctt gccataaact
     2461 cgctcaacgt catagggcga aaacagcgcc atctgcgcat tctcttttgc cagatggaaa
     2521 gtgatatccg ggatcaccac gcccagcgac agtgttttaa tgcggatttt ttcgtcggca
     2581 ttttcccgtt tcgtgtcgag aaaacgcaga atatcgggat gatgagcatg taaatagact
     2641 gcacctgccc cctgacgcgc gccgagttgg ttggcatagg aaaatgcgtc ttccaacatt
     2701 ttcatcaccg gaattacgcc agaagattga ttttcaatac gtttaattgg cgcgcccgct
     2761 tcccgcagat tcgacagcaa aaatgctacg ccgccgccgc gtttcgacag ctgcagtgcg
     2821 gaatttaccg cccgaccaat cgactccata ttgtcttcaa tacgcagcaa aaaacaggaa
     2881 accagttcgc cgcgctgctg cttaccgcag ttgaggaatg ttggcgtggc tggctgaaag
     2941 cgtcctgaca gcatttcatc ggtcagttgc aacgccagcg tctcatcgcc ttgtgccagc
     3001 gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca gataacgttt accgtcgaat
     3061 gtcttcaacg tatagctggt gtaaaacttc catgccccga ggaatgtctg gaaacgaaaa
     3121 ccgctggtgt gtgcgtgggc aaacagcgta atgacaaagt cgcgagagta gcgattaaga
     3181 acgctttcat catagtaacc ttcgttgacc agccagttca ggcgctgctg ctggctactg
     3241 aaggtcacac tgttcggacg cacatgcgtc gcaataaagg cgtcaacggc ctggcggtct
     3301 ttatcgaact gaatgcgacc tgcgctatcg taaagattaa gcatcgcatt cagcgcgtgg
     3361 taatccatcg tttcctgcgt caggcattct gcggttgtcg ttgccaaaat tcggttactc
     3421 ctttacgaac gttttcgata tcgctttgcg tacccatgag ttcaaaacgg tacagccacg
     3481 gcacgccgca tttccgggca atcacatctc cggcgcggcc atacgcctca ccaaagttgc
     3541 gattaccaga agcaataacg ccgcgaagca acgcccggtt gtgctcgtcg tttaaaaagc
     3601 gaattacctg tcgtggcacc gcgccagccg taccgccgcc gccgtaagag ggcacgatca
     3661 ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg
     3721 gcagacctaa acgttcgata aaacgctgcg tgttttcgga gctgctggag aagtagacga
     3781 gctggctcat gcactggccg cgtgtggcgc tggatgcaga cggttaatca tgtccggacg
     3841 gaaaccagac cagctaagat cgccagcaat cactaccggc aactgacgaa agccctgagc
     3901 acgcaacgct tccgctgctt caggaacgcg atcgacatta atcatttcaa aatcaaagcc
     3961 ccggttttcc atcgcccgtt tggtggcgtg gcactgaacg caatcgttac gagtataaat
     4021 agttatgcgc atgattcgta tttccgttta aaatgaagat acggcgcgat gatacgcgtc
     4081 gggttgtctc tctgttgata cagagatact agatgtagtt gaaaaaagat tcaactacac
     4141 aatatatagc aaatagtgat cgaaattacc ctggatatga gcgtgacggg ggagggggat
     4201 ttttgtgatt caccaggcaa aaagaaaccc cgaagacagg cttcggggtc aaagacgcgt
     4261 atttattatc atttttgcac tacgatttgc gcatgcttaa cagtgcgccg ataaaaatac
     4321 ctaccgcagc tgctgtaccc acgctacacc agggtctttc acgaacaaaa gaatccgctc
     4381 agccaacggc atcgcgcgcg gcttgctgga cgcgagtacg accatgcatt cgcgccctgg
     4441 tttctttcag caacgcctga gctttactgc gtgcggcttc agcttcccct ttggcgtcgc
     4501 tgccccagga cttcaataca gattcgaggc tgtcagctaa ttgattgaca tcattctgga
     4561 tatcctgcac gccatcatca acgtcgttgc ggttcggtcg gttaaacata tgatccccca
     4621 ttagaattcg atgtaatttt cagtgtagac cataattttc ttaactgagg ttcatcgcgg
     4681 ctttatggac ttttctgaaa gcgttgtgaa tgcgcaatca ggtaaggtag gcccgcggtt
     4741 gaaaataacg aggatcaaat atgtatttac gaccagacga ggtggcgcgc gtacttgaaa
     4801 aagtcggttt tactgtcgat gtggtaaccc agaaagcgta tggttaccgc cgtggggaaa
     4861 attatgtcta tgttaatcgc gaagcgcgaa tgggacgtac cgctctggta attcatccaa
     4921 cattaaaaga acgcagttca acactggctg aacccgcttc cgatattaaa acttgcgatc
     4981 attaccagca atttccgctc tatttagcag gcgagcgaca cgagcattac ggtatcccgc
     5041 atggctttag ttcgcgtgtt gcgcttgaac gttatttgaa tggtttattt ggcgaagcca
     5101 gttaaagacg cgactggcga tgccagtcgc gaaaagaaga gtcaggcttt tacctgctgg
     5161 taacggctga ctttaaacag tcggcggcaa taatcgagga agtagccata aaccgccccc
     5221 atcaacatcg aaacaacgat gtttgaactg accgccgcca taatctgatg ccagtctgcg
     5281 cccaccacta acaagatcgc cacatacacc ggtgactgga acgtcacata agccaggata
     5341 tcagccagat tttttatcca gcccgacggg ctaactttgc gtgccgcgcg cataaacaga
     5401 tcgcggtaca taccgtatgg ccatgcaatt aagatgttca ccggaatcgc taccaatctg
     5461 gaataaaaag actgttcgaa gctcattccg gagaggaaaa cttcaataca catgttcacg
     5521 acagaacagt aaacaaccat cgcgaacgta tctgcaactg catgacgcaa gcgtgactgc
     5581 ggtgagaaca tgctaatgct ccttaataaa agcgtaatgg gatgttaatg gagatgaaag
     5641 cagtggatct cattgccatc tgcatttttg cata
//
LOCUS       DAZPEU010000217         5659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPEU010000217 DAZPEU010000000
VERSION     DAZPEU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.219"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1266
                     /gene="hpaG"
                     /locus_tag="ACHU2L_003654"
     CDS             <1..1266
                     /gene="hpaG"
                     /locus_tag="ACHU2L_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR1358332.1"
                     /translation="ALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNTVIGCGEPI
                     PFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESFYRPAIKAK
                     CRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSALSEFATLNP
                     GDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTLPHPHGTLF
                     ALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYEAELVVVIG
                     KQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPMLSTIVPKEA
                     IPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIATGTPKGLS
                     DVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1263..2729
                     /gene="hpaE"
                     /locus_tag="ACHU2L_003655"
     CDS             1263..2729
                     /gene="hpaE"
                     /locus_tag="ACHU2L_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1358333.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2731..3582
                     /gene="hpaD"
                     /locus_tag="ACHU2L_003656"
     CDS             2731..3582
                     /gene="hpaD"
                     /locus_tag="ACHU2L_003656"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR1358334.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3592..3972
                     /locus_tag="ACHU2L_003657"
     CDS             3592..3972
                     /locus_tag="ACHU2L_003657"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR1358335.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4040..4843
                     /gene="hpaH"
                     /locus_tag="ACHU2L_003658"
     CDS             4040..4843
                     /gene="hpaH"
                     /locus_tag="ACHU2L_003658"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR1358336.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4854..5657
                     /gene="hpaI"
                     /locus_tag="ACHU2L_003659"
     CDS             4854..5657
                     /gene="hpaI"
                     /locus_tag="ACHU2L_003659"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR1358337.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
BASE COUNT         1339 a         1579 c         1625 g         1116 t
ORIGIN      
        1 gcgttgaacc atcgcagcca acttgatgcc tggcaggaag cgttccagca atctccctac
       61 aaagccccgc ctaaaactgc ggtctggttt attaaaccgc gcaatacggt gattggttgc
      121 ggtgaaccga ttccctttcc acagggtgaa aaagtactga gcggtgcgac tgttgcgctg
      181 attgtgggga aaacggcgac gaaagtacgt gaagaagatg cggcagagta catcgccgga
      241 tatgcgctgg ataacgacgt cagcctgccg gaagagagct tttaccgtcc ggcaatcaaa
      301 gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg tggctctcag caatgtcgat
      361 aatctgacta tctataccga gatcaacggg cgtcctgccg atcactggaa taccgccgat
      421 ttacaacgta acgccgcaca gctgctgagc gccctgagcg aatttgccac gctgaatcca
      481 ggcgatgcta ttctgctcgg cacgccacag gcgcgcgtgg aaatacagcc aagcgatcgc
      541 gtgcgtgttc tcgcagaagg tttcccgccg ctggaaaatc cggtagtgga cgaacgtgaa
      601 gtgaccacgc gcaagagctt cccaacgctg ccacacccgc acggtacgct gtttgccctc
      661 ggcctgaact acgccgacca cgccagcgaa ctggaattta agccaccgga agagccgctg
      721 gtgttcctga aagcgccgaa taccctcact ggcgacaacc agacctccgt gcgcccgaac
      781 aatattgaat acatgcacta cgaagcggag ctggtggtag ttattggcaa gcaggcgcgt
      841 aacgtcagcg aagccgatgc catggattat gtcgcgggct acaccgtgtg taacgactac
      901 gccattcgcg actatctgga aaactactac cgccctaacc tgcgggtcaa aagccgcgac
      961 ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg cgatcccgga cccgcataat
     1021 ctgacccttc gcaccttcgt caacggcgag ttacgccagc aaggcaccac cgccgatctg
     1081 atcttcagcg tgcccttcct gatcgcctac ttaagcgaat ttatgaccct gaatccgggc
     1141 gacatgatcg ccaccggcac accaaaaggc ttatctgacg tggtgcctgg cgatgaagta
     1201 gtggtggaag tagaaggcgt gggccgcctg gtgaaccgaa ttgtgagtga ggaaacagcg
     1261 aaatgaaaaa agtaaatcat tggatcaacg gcaaaaatgt tgcaggtaac gactacttcc
     1321 agactaccaa tccggcaacg ggtgaagtgc tggcggatgt ggcctctggc ggtgaagcgg
     1381 agatcaatca ggcggtagcg gcagcgaaag aggcgttccc gaaatgggct gatctgccga
     1441 tgaaagagcg tgcgcgcctg atgcgccgtc tgggcgatct gatcgaccag aacgtgcctg
     1501 agatcgccgc gatggaaacc gcggacacgg gcctgccgat ccatcagacc aaaaatgtgt
     1561 tgatcccacg cgcttctcac aactttgaat ttttcgcgga agtctgccag cagatgaacg
     1621 gcaagaccta tccggttgac gacaagatgc tcaactacac gctggtgcaa ccggtaggcg
     1681 tttgtgcgct ggcgtcgccg tggaacgtgc cgtttatgac cgccacctgg aaggtcgcgc
     1741 cgtgtctggc gctgggcaat accgcggtgc tgaagatgtc ggaactctcc ccgctgaccg
     1801 ccgaccgcct gggtgagctg gcgctggaag ctggtattcc ggcgggcgtg ctaaacgtgg
     1861 tacagggcta cggcgcaacc gcaggcgatg cgctggtccg tcatcatgac gtgcgtgccg
     1921 tgtcgttcac cggcggtacc gccaccgggc gcaacatcat gaaaaacgcc gggctgaaaa
     1981 aatactccat ggagctgggc ggtaaatcac cggtgctgat ttttgaagat gccgatattg
     2041 agcgtgcgct ggacgccgcc ctgttcacca tcttctcaat caacggcgaa cgctgcaccg
     2101 ccggttcgcg catctttatt cagcagagca tctatccaga attcgttaaa cgctttgccg
     2161 aacgcgccaa ccgtctgcgc gtgggcgatc cgaacgatcc gaatacccag gttagggcgc
     2221 ttatcagtca gcaacactgg gaaaaagtct ccggctatat ccgtctcggc attgaagaag
     2281 gcgcaaccct gttggcgggc ggcccggata aaccgtccga cctgcctgcg cacctgaaag
     2341 gcggcaactt cctgcgccca acggtgctgg cggacgtaga taaccgtatg cgcgttgccc
     2401 aggaagagat tttcgggccg gtcgcctgcc tgctgccgtt taaagacgaa gccgaaggct
     2461 tacgcctggc aaacgacgtg gagtacggcc tcgcgtcgta catctggaca caggatgtca
     2521 gcaaagtgtt acgcctggcg cgtggcattg aagcaggcat ggtgttcgtc aacacccaga
     2581 acgtgcgcga cctgcgccag ccatttggcg gcgtaaaagc ctccggcacc gggcgtgaag
     2641 gcggtgagta cagcttcgaa gtgttcgcgg aaatgaagaa cgtctgcatt tccatgggcg
     2701 accatccaat tccgaaatgg ggagtctgat atgggtaagt tagcgttagc cgccaagatc
     2761 acgcacgtac cgtcgatgta tctctctgaa ctaccaggaa aaaaccacgg ttgtcgccag
     2821 ggtgcgatcg acgggcataa agagatcagc aagcgttgcc gggaaatggg cgtcgatacc
     2881 attatcgttt tcgatactca ctggctggtc aacagtgctt atcacatcaa ctgtgcagac
     2941 cattttgaag gcgtctacac cagcaacgag ctgccgcatt ttattcgcga catgacctac
     3001 aactacgagg gcaacccgga gttggggcag ctaattgccg aggaagcctt aaagctcggc
     3061 gtgcgggcaa aagcgcacaa tattcccagc ctgaaactgg aatacggcac gctggtaccg
     3121 atgcgctaca tgaatgaaga caagcacttc aaagtggtct ccatttcggc tttctgcacg
     3181 gttcacgatt ttgccgacag ccgcaagctg ggcgaagcga ttctgaaagc gatcgaacag
     3241 tacgacggca ccgtggcggt ccttgccagc ggttcgttat cgcaccgctt tattgacgat
     3301 cagcgtgcgg aagaagggat gaacagctac acccgcgagt tcgaccgcca gatggacgag
     3361 cgtgtggtga agctgtggcg cgaaggccag ttcaaagagt tttgcaatat gctgccggag
     3421 tacgccgact actgctacgg cgaaggcaat atgcacgaca cggtgatgct gctggggatg
     3481 ctcggctggg ataaatacga cggcaaggtg gagtttatta ccgagctgtt cccaagctct
     3541 ggcaccggtc aggttaacgc tgttttcccg ctgcccgcgt aaggacgttt tatgccgcac
     3601 tttatcgtgg aatgtactga aaatattcgt gaagaagccc gcttacctga gctgtttgcc
     3661 agtgttaaca ctgcactggc ggcaacgggg atttttccgc ttgggggaat acgtagccgg
     3721 gcgcactgga tcgatacctg gcaaatggct gatggcaagc acgattacgc ctttgtgcat
     3781 atgacgctaa aaatcggcag cggacgcagt ctggaaagcc gtcaggaagt gggcgaaatg
     3841 ctgtttgatc tgattaaaac gcacttcgcc tcactgatgg agagccgtta cctagcgctt
     3901 tcatttgaga ttgcagagct gcacccgacg ttgaatttca aacagaacaa cgtacacgcg
     3961 ttgtttaaat aaatttgcac cagcttccag gccggatgat aaggcgctgc cgcatccggc
     4021 aatgccacag gatatcgcta tgttcgataa acacacccac accctgatcg cccagcgtct
     4081 ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc tcgctggatt acccggagat
     4141 caccatcgaa gacgcctacg ccgtgcagcg cgaatgggtt cgcctgaaaa tcgccgaaag
     4201 tcgcacgctg aaaggccaca aaatcggtct gacctcgaaa gcgatgcagg caagctcgca
     4261 gatcagcgaa ccggattacg gtgcactgct ggacgacatg ttcttccacg atggcagcga
     4321 tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg gagctggctt ttgtgctggc
     4381 aaaaccgctg cgtgggccaa actgcacgct gttcgacgtt tacaacgcca cggactatgt
     4441 gatcccggcg ctggagctga tcgacgctcg ctgccacaac atcgatccgg aaacccagcg
     4501 cccgcgcaaa gtattcgaca ccatttccga taacgccgcc aacgccggag tgatcctcgg
     4561 aggtcgtccg atcaaaccag acgaactgga tctgcgttgg atctccgccc tgatgtatcg
     4621 caatggcgtg attgaagaaa ccggcgtcgc cgctggtgtg ctgaatcatc cggcaaacgg
     4681 cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta caactggaaa ccgggcaaat
     4741 cattctcggt ggttcgttca ccctcccggt tccggcgcgt aagggcgaca ccttccacgt
     4801 cgattacggc aacatgggct ccattagctg ccgctttgtt taaggagaga acgatggaaa
     4861 acagttttaa agcggcgctg aaagcaggcc gtccgcagat tggattatgg ctggggctga
     4921 gcagcagcta cagcgcggag ttactggccg gaccaggatt cgactggtta ttgatcgacg
     4981 gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca actacaggcg attgcgccct
     5041 accccagcca gccagtggta cgcccatcgt ggaacgatcc ggtgcaaatc aaacaactgc
     5101 tggacgtagg cacacaaacc ttactggtgc cgatggtaca aaacgccgac gaagcccgta
     5161 aagcggtacg cgccacccgt tatccccccg ccggtattcg cggtgtgggc agtgcgctgg
     5221 ctcgcgcctc gcgctggaat cgcattcctg attacctgca aaaagccaac gatcaaatgt
     5281 gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa cttaccgcag attctggacg
     5341 tggaaggggt cgacggcgtg tttatcggcc cggcggatct gagcgccgat atgggttatg
     5401 ccggtaatcc gcagcacccg gaagtacagg ccgccattga gcaggcgatc gtgcagatcc
     5461 gcgaagcggg caaagcgccg gggatcctga tcgccaatga gcaactggca aaacgctatc
     5521 tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac caccctgctc gcccgcgccg
     5581 ccgaagcgct ggcagcacgg tttggtaata gttcttctat ttcgatggcg aagcaaaaca
     5641 acaactctgt ctactaaac
//
LOCUS       DAZPEU010000218         5599 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPEU010000218 DAZPEU010000000
VERSION     DAZPEU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.220"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..410
                     /locus_tag="ACHU2L_003660"
                     /pseudo
     CDS             1..410
                     /locus_tag="ACHU2L_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="ACHU2L_003661"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="ACHU2L_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="ACHU2L_003662"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="ACHU2L_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR1358338.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1401 c         1332 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt agggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tctacagcgc aggagagctg
      721 ttgcaggaac tacgtaaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag acctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggcata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggac ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gaccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc ggcgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaggaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tactcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DAZPEU010000219         5590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPEU010000219 DAZPEU010000000
VERSION     DAZPEU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.221"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..279)
                     /locus_tag="ACHU2L_003663"
     CDS             complement(1..279)
                     /locus_tag="ACHU2L_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358339.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHU2L_003664"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHU2L_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1358340.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHU2L_003665"
     CDS             complement(1254..1670)
                     /locus_tag="ACHU2L_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358341.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHU2L_003666"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHU2L_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHU2L_003667"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHU2L_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHU2L_003668"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHU2L_003668"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHU2L_003669"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHU2L_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR1358342.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHU2L_003670"
     tRNA            3996..4071
                     /locus_tag="ACHU2L_003670"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHU2L_003671"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHU2L_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAZPEU010000220         5557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPEU010000220 DAZPEU010000000
VERSION     DAZPEU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.222"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..641
                     /locus_tag="ACHU2L_003672"
     CDS             <1..641
                     /locus_tag="ACHU2L_003672"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR1358343.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHU2L_003673"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHU2L_003673"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR1358344.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHU2L_003674"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHU2L_003674"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR1358345.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagatatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAZPEU010000221         5523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPEU010000221 DAZPEU010000000
VERSION     DAZPEU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.223"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            567..1556
                     /gene="araF"
                     /locus_tag="ACHU2L_003675"
                     /pseudo
     CDS             567..1556
                     /gene="araF"
                     /locus_tag="ACHU2L_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            1626..3140
                     /gene="araG"
                     /locus_tag="ACHU2L_003676"
     CDS             1626..3140
                     /gene="araG"
                     /locus_tag="ACHU2L_003676"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR1358346.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3155..4141
                     /gene="araH"
                     /locus_tag="ACHU2L_003677"
     CDS             3155..4141
                     /gene="araH"
                     /locus_tag="ACHU2L_003677"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1358347.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4308..5108
                     /gene="otsB"
                     /locus_tag="ACHU2L_003678"
     CDS             4308..5108
                     /gene="otsB"
                     /locus_tag="ACHU2L_003678"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR1358348.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            5083..5523
                     /locus_tag="ACHU2L_003679"
                     /pseudo
     CDS             5083..5523
                     /locus_tag="ACHU2L_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
BASE COUNT         1364 a         1271 c         1475 g         1413 t
ORIGIN      
        1 ttaatgatag tgctttttgt atcttgttga tattaatcga tgaatttgaa ttcatgatga
       61 agtgatgatt ttataaaacg ttttttcatt tttgcgagat ggctctcatt atacgtgttc
      121 tgttaattaa tgagcacagt gataattaat ggcgaaatga atgacgcgca ttttccacat
      181 ctttgagttg tggttattac accatttcaa aaactcaacg ccaggtaatg cggcctattg
      241 actggttaaa aagaagacat cccgcatggg taccgaagac aacaaggatt tccaggctaa
      301 tcttatggat taatctgctg tgcattcaac aatttgtctg acaaattggc ttttccttat
      361 gtcttttccc gctaaattta tgcacgttct cactgtaatt ctgcgatgtg atattgctct
      421 cctatggaga attaatttct cgctaaaact atgtcaacac ggtcacttat cttttagata
      481 aaaggtaatg ctttgttttc cgattaattt aacgaatgtc attcgttttt gccctacaca
      541 aaacgacact aaagctggag agaaccatgc acaaatttac taaagccctg gcagccattg
      601 gtctggcagc cgttatgtca caatccgcta tggcggagaa cctgaagctc ggttttctgg
      661 tgaagcaacc ggaagagccg tggttccaga ccgaatggaa gtttgccgat aaagccggga
      721 aggatttagg gtttgaggtt attaagattg ccgtgccgga tggcgaaaaa acattgaacg
      781 cgatcgacag cctggcagcc agtggcgcaa aaggtttcgt tatttgtact ccggacccga
      841 aactcggctc tgccatcgtc gcgaaagcgc gtggctacga tatgaaagtc attgcagtgg
      901 atgaccagtt tgttaacgcc aaaggtaagc caatggatac cgttcctctg gtgatgatgg
      961 cggcgactaa aattggcgaa cgtcagggct aggaactgta taaagagatg cagaaacgtg
     1021 gctgggatgt caaagaaagc gcagtgatgg cgattaccgc caacgaactg gataccgccc
     1081 gccgccgtac aacgggttct atggacgcac tgaaagctgc aggattcccg gaaaaacaaa
     1141 tttatcaggt acctaccaaa tctaacgaca tcccgggggc atttgacgct gccaactcaa
     1201 tgctggttca acatccggaa gttaaacatt ggctgatcgt cggtatgaac gacagcaccg
     1261 tacttggcgg cgtacgtgca accgaaggtc agggctttaa agcagccgat atcatcggta
     1321 ttggtattaa cggtgtggat gcagtaagcg aactgtctaa agcacaggca accggcttct
     1381 acggttccct gctgccaagc ccggacgtac atggctataa atccagcgaa atgctttaca
     1441 actgggtagc aaaagacgtt gaaacgccaa aatttaccga agttacagac gtggtactga
     1501 tcacgcgtga caactttaaa gaagaactgg agaaaaaagg tttaggcggt aagtaatttg
     1561 ccggaaaaat tcccctctgc atgatgcaga gggggtgtga acgaccagtg attcacggag
     1621 acgttatgca acagtctacc ccgtatctct catttcgcgg catcggtaaa acgtttcccg
     1681 gcgttaaggc gctgacggat attagttttg actgctatgc cggtcaggtt catgcgttga
     1741 tgggtgaaaa tggcgcagga aaatcaactc tcttaaaaat cctcagcggc aactatgcgc
     1801 caaccacggg ttctgtagtg attaatgggc aggaaatgtc cttttccgac acgaccgcag
     1861 cacttaacgc gggtgtggcg attatttacc aggaactgca tctcgtgccg gaaatgaccg
     1921 tcgcggaaaa catctatctc ggccagctgc cgcataaagg cggcattgtg aatcgctcat
     1981 tgctgaatta tgaggcgggt ttacaactta aacatcttgg tatggatatt gacccggaca
     2041 cgccgctgaa atatctcgcc attggtcagt ggcagatggt tgaaatcgcc aaagcgctgg
     2101 cgcgtaacgc taaaattatc gcctttgatg agccaaccag ctccctttct gcccgcgaaa
     2161 tcggcaatct tttccgcgtt attcgtgaac tgcgaaaaga ggggcgggtg atcttatacg
     2221 tttctcaccg tatggaagaa atatttgccc tcagcgatgc catcactgtc tttaaagatg
     2281 gacgttatgt caaaaccttt accgatatgc agcaggttga ccacgacgcg ctggtgcagg
     2341 cgatggtcgg gcgcgacatt ggcgatatct acggctggca acctcgtagt tatggcgagg
     2401 agcgcctgcg tcttgatgct gtgaaagcac taggcgtgcg tacgccaata agtctggcgg
     2461 ttcgcagtgg tgaaattgtt gggctgtttg gtctggtagg ggcggggcgt agcgaattaa
     2521 tgaaaggctt gtttggcggg acgcaaatca ccgccggtca ggtttatatc gaccaacagc
     2581 cgatcgatat tcgtaaaccg agccacgcca ttgccgcagg catgatgctc tgcccggaag
     2641 ctcgcaaagc ggaaggcatt attcccgtgc actccgttcg cgacaatatc aacatcagtg
     2701 ccagacgcaa acatgtgctc ggcggttgtg taatcaacaa cagttgggaa gaaaacaatg
     2761 ccgatcagca cattcgttcg ctcaacatca aaacgccggg cgcggagcaa ctgatcatga
     2821 atctctcagg cggaaatcag caaaaagcca ttctgggccg ctggttatcg gaagagatga
     2881 aggtcatttt gctggatgaa cctacgcgcg gcattgatgt tggcgctaag cacgaaatat
     2941 ataacgtaat ttatgcgctg gcggcgcagg gcgtggcggt gctgtttgcc tccagcgact
     3001 tacctgaagt cctcggcgtt gccgaccgga ttgtggtgat gcgggaaggc gaaatcgccg
     3061 gtgaattgtt acacgagcag gcagatgagc gtcaggcact gagccttgcg atgcctaaag
     3121 tcagccaggc agttgcctga gtaaggagag tatgatgtct tctgtttcta catcggggtc
     3181 tggcgcacct aagtcgtcat tcagcttcgg gcgtatctgg gatcagtacg gcatgctggt
     3241 ggtgtttgcg gtgctcttta tcgcctgtgc catttttgtc ccaaattttg ccaccttcat
     3301 taatatgaaa gggttgggcc tggcaatttc catgtcgggg atggtggcgt gtggcatgtt
     3361 gttctgcctt gcttccggtg actttgacct ttctgtcgcc tccgtaattg cctgtgcggg
     3421 tgtcaccacg gcggtggtta tcaacctgac tgaaagcctg tggattggcg tggcagcggg
     3481 tttgttgctg ggcgttctct gtggcctggt caatggcttt gttatcgcca aactgaaaat
     3541 aaatgctctg atcacgacac tggcaacgat gcagattgtt cgaggtctgg cgtacatcat
     3601 ttcagacggt aaagcggtcg gtatcgaaga tgaaagcttc tttgcccttg gttacgctaa
     3661 ctggttcggt ctgcctgcgc caatctggct caccgtcgcg tgtctgatta tctttggttt
     3721 gctgctgaat aaaaccacct ttggtcgtaa taccctggcg attggcggga acgaagaggc
     3781 tgcgcgtctg gcgggtgtac cggttgttcg caccaaaatt attatctttg ttctctctgg
     3841 cctggtatct gcgatagccg gaattattct ggcttcacgt atgactagtg ggcagccaat
     3901 gacgtcgatt ggttatgagc ttattgttat ctccgcctgc gttttaggtg gcgtttctct
     3961 gaaaggtggc atcggaaaaa tctcatatgt ggtggcgggt atcttaattt taggcaccgt
     4021 ggaaaacgcc atgaacctgc ttaatatttc tcctttcgcg cagtacgtgg ttcgcggctt
     4081 aatcctgctg gcagcggtga tcttcgaccg ttacaagcaa aaagcgaagc gcactgtctg
     4141 atgctttttt ccacaacaac ttaacgtttt ttcccaccac agccagccgc cacaacggtt
     4201 ggctgttctt cgttgcaaat ggcgacctcc gtcacactgt ctatacttac atgtctgtaa
     4261 agcgcggtct gcgcaacaca ataagaaaag agaaggagga gagccgggtg acagaaccgt
     4321 taaccgaaac ccctgaacta tccgcgaaat atgcctggtt ttttgatctt gatggaacgc
     4381 tggcggaaat caaaccgcat cccgatcagg tcgtcgtgcc tgacaatatt ctgcaaggac
     4441 tacagctact ggcaaccgca agtgatggtg cattggcatt gatatcaggg cgctcaatgg
     4501 tggagcttga cgcactggca aaaccttatc gcttcccgtt agcgggcgtg catggggcgg
     4561 agcgccgtga catcaatggt aaaacacata tcgttcatct gccggatgcg attgcgcgtg
     4621 atattagcgt gcaactgcat acggtcatcg ctcagtatcc cggcgcggag ctggaggcga
     4681 aagggatggc ttttgcgctg cattatcgtc aggctccgca gcatgaagac gcattaatga
     4741 cattagcgca acgtattact cagatctggc cacaaatggc gttacagcag ggaaagtgtg
     4801 ttgtcgagat caaaccgaga ggtaccagta aaggtgaagc gattgcagcc tttatgcagg
     4861 aagctccttt tatcgggcga acgcccgtat ttctgggcga tgatttaacc gatgaatctg
     4921 gcttcgcagt cgttaaccga ctgggcggaa tgtcagtaaa aattggcaca ggtgcaactc
     4981 aggcatcatg gcgactggcg ggtgtgccgg atgtctggag ctggcttgaa atgataaccg
     5041 ccgcattaca acaaaaaaga gaaaataaca ggagtgatga ctatgagtcg tttagtcgta
     5101 gtatctaacc ggattgcacc accagacgag cacgccgcca gtgccggtgg ccttgccgtt
     5161 ggcatactgg gggcactgaa agccgcaggc ggactgtggt ttggctggag tggtgaaaca
     5221 gggaatgagg atcagccgct aaaaaaggta acattacgtg ggcctctttt aacctcagcg
     5281 aacaggacct tgacgaatac tacaaccaat tctccaatgc cgttctctgg cccgcttttc
     5341 attatcggct cgatctggtg caatttcagc gtcctgcctg ggacggctat ctacgcgtaa
     5401 atgcgttgct ggcagataaa ttactgccgc tgttgcaaga cgatgacatt atctggatcc
     5461 acgattatca cctgttgccc tttgcgcatg aattacgcaa acgtggggtg aataatcgca
     5521 ttg
//
LOCUS       DAZPEU010000222         5522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPEU010000222 DAZPEU010000000
VERSION     DAZPEU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.224"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            22..450
                     /gene="uspG"
                     /locus_tag="ACHU2L_003680"
     CDS             22..450
                     /gene="uspG"
                     /locus_tag="ACHU2L_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR1358349.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(571..2136)
                     /gene="ahpF"
                     /locus_tag="ACHU2L_003681"
     CDS             complement(571..2136)
                     /gene="ahpF"
                     /locus_tag="ACHU2L_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR1358350.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2381..2944)
                     /gene="ahpC"
                     /locus_tag="ACHU2L_003682"
     CDS             complement(2381..2944)
                     /gene="ahpC"
                     /locus_tag="ACHU2L_003682"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR1358351.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3316..4062
                     /gene="dsbG"
                     /locus_tag="ACHU2L_003683"
     CDS             3316..4062
                     /gene="dsbG"
                     /locus_tag="ACHU2L_003683"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR1358352.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4272..5174
                     /locus_tag="ACHU2L_003684"
     CDS             4272..5174
                     /locus_tag="ACHU2L_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1358353.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5321..5522
                     /locus_tag="ACHU2L_003685"
                     /pseudo
     CDS             5321..5522
                     /locus_tag="ACHU2L_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1465 a         1342 c         1249 g         1466 t
ORIGIN      
        1 gattaacagg gagaaagggt tatgtataag gcaatcatta tgccagttga tgtatttgaa
       61 atggaattga gcgacaaagc tgttcgccac gctgaattcc tcgcccagga tgacggagtt
      121 attcatctac ttcacgtact acccgggtca gccagcctga gcctgcaccg ttttgccgct
      181 gatgtgcgtc gttttgaaga gcatctgcaa catgaagcag aagaacgtct gcaaacgatg
      241 gtcagccact tcaccatcga tccttcccgc attaaacaac atgtccgttt tggtagcgtg
      301 cgggatgaag tcaatgagtt ggcagaagaa ctgggggctg atgttgtagt tatcggttct
      361 cgcaacccat cgatttcgac ccatctgtta ggttctaacg cctcgagcgt gatccgccac
      421 gccaatctgc ctgtactggt cgttcgctaa cagataaaag tatttatgcc gccctgcctg
      481 gaacatcccg gcagggcttt ttaatgccat aaaaaagccg ccaggtttga ccccggcggc
      541 taagcaattg caggtgaatc ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc
      601 aaaagcactc agagaggctt tggcaccttc gccagtggcg atgatgatct gcttgtacgg
      661 aaccgtcgta cagtcacccg ctgcgaacac gcctttgacg ttggtttcac atttcgcatc
      721 aatgataatc tcgcccatgc ggttacgttc gactgcgcct tccagccagt tggtgttcgg
      781 cagcagacca atctggacaa aaataccggc cagttcgatg ttgtgaatat tgccgctgac
      841 acgatctcga tattccagac caacgacttt gctgccgtcg cctttcactt ccgtggtttg
      901 cgcattcaga ataatgtcga cgtttttcag gctgcgcagt ttgtcctgca gaacctggtc
      961 ggctttcatt tctggcgcaa attccagcag cgttacgtgc tcaacgatac ccgccaggtc
     1021 gattgccgct tccacgccgg agttaccgcc gccgataacc gctacgcgtt tgcctttaaa
     1081 cagcgggccg tcgcagtgcg ggcagtaggt cacgccttta gtgcgatact gatcttcgcc
     1141 cggaacgttc atgttgcgcc attttgcacc cgtcgccacg ataatgctgc gtgctttcag
     1201 taccgcgcca gaagctgttt caatctggtg tagaccacct tcaaccgctg ccgggatcag
     1261 tttgctggcg ctctggctgt cgatcacatc aacgtcgtat tcatcaacgt gaactttcag
     1321 tgcgcctgcc aacttctgcc cttcagtctt cggtacagaa atgtagtttt cgatatcaac
     1381 ggtatcgagg atctgaccac caaaacgttc gcccatcaga ccggtacgga tgcctttacg
     1441 tgcggagtaa attgctgccg ctgcacccgc cgggccggaa ccaacgatta atacgtcata
     1501 agcatcacgc ttgttcagct cttctgccgc acgattttcc gcgccagtat caattttggc
     1561 aacgatttca gtcaacgtca tgcggccctg accaaactct ttcccgttta cgaacactgc
     1621 cggaacgccc atcacgttgc gatcggtgat ttcgttctgg aaggtgccgc cgtcaattgc
     1681 agtgtgcttg atgcgcgggt tcaatacgct catcaggttc agcgcctgca ccacgtccgg
     1741 gcagttatgg caagagagcg agtaataggt ttcgaattca aaatcaccgt caatatggcg
     1801 aatctgctcc agcagagact gcgcttcttt cgacggatga ccaccggtcc acagcaacgc
     1861 cagtaccagc gaggtgaact cgtggcccag cggggagcct gcaaaacgtg gtccctggtt
     1921 ggaacctggg ttggtgatca ggaaagacgg cttacgcact ggcaagctgt tatcttcttt
     1981 aaaggtgact ttgtctgaca gttctgcgat ttcagccaac agttccttga tttctgccga
     2041 tttagcgctg tcatccagcg tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta
     2101 agccttgagt tgagttttca tatttgtgtc gagcatgttt atctcctggg ctttaaacat
     2161 catcatgcaa gcggcctctg cggctacctg gatgcagctt gcatcatggt gcggggaaga
     2221 aaatcgggtg ccgcagcacc cgaagaatta ggtgaatttc cgaagtattt cgcgtcagag
     2281 caaggcgact agcctgtgaa tccccgggag cttacacaag taagtgaccg gggtgagcgg
     2341 gcgacgccaa cgccgctatg gcgtgaaaga cgaaggaaat ttagatttta ccaaccaggt
     2401 ccagagacgg agccagagtt gcttcacctt ctttccattt agccgggcaa acttcacctg
     2461 ggtgagaagc tacgtactga gctgctttga ttttacgcag cagatcagac gcgtcacggc
     2521 caatgccttc agcggtaact tcgattgcct ggatgatacc ctgcgggtca acaacgaagg
     2581 tcgcacggtc agccagacct tcatcttcac gcatgttgtc gaagttacgg gtcagggcgc
     2641 cagtcgggtc gccgatcatc gcatatttga ttttagcgat ggtttcagag ctgctgtgcc
     2701 atgctttgtg ggtgaagtga gtatcggtag atactgcgta tacgtctacg cccagtttct
     2761 gcagttcttc gtagtggtca gcaacgtcac ccagttcggt cgggcatacg aaagtaaagt
     2821 cagccgggta gaagaagaag acgctccagc ggccttcggt atctttttcg gtgatttcga
     2881 tgaattcgcc gtttttgaat gcctggtttt taaaaggttt aattttggtg ttaatcaagg
     2941 acatctatac ttcctccgtg ttttcgatga gatgtaaggt aaccaatttt tgccgattcg
     3001 gctaatgcgt ttccattatc aaatcgataa gttttacctt acaaccttcg taagacaact
     3061 ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat ggcccccttt ccaggtgacc
     3121 gtgtaatctg aactaccctt tggtatcttc agcgcctgcc tgcaggctga ggtgttgccc
     3181 ttacaaatgc aacaacgaca tggattacaa caccctcata aacaaagggc aatcacctga
     3241 tctaagctct tacctatgac agtgataggt tatgcctttt actcgacttt tgcactgact
     3301 gaaaagaaca aattaatgtt aaaaaagata cttttactgg ctctgcttcc tgcaatcgcc
     3361 ttcgcagagg aacttccttc cccagtaaaa gcgattgaaa aacagggcat tacaatcatc
     3421 aaaacgttcg atgcccccgg aggaatgaaa ggttatctcg gaaagtatca ggatatgggc
     3481 gtcaccatct acctgactcc cgatggtaag cacgctatct ctggttacat gtacaacgag
     3541 aaaggtgaaa acctgagtaa cacacttatc gaaaaagaaa tttacgcacc agccggacgc
     3601 gaaatgtggc aacggatgga acaatcccac tggctcctcg acggtaaaaa agatgcgccg
     3661 gtcattgtct acgtcttctc cgatccgttc tgcccatatt gtaaacagtt ctggcagcag
     3721 gcgcgcccgt gggtagattc tggcaaagtg caattaagaa cattgttggt tggggttatc
     3781 aagccagaaa gcccggcgac agcagcggca attcttgcct ccaaagatcc cgcaaaaacc
     3841 tggcaacaat atgaagcctc tggttgcaag cttaagctaa acgtgcctgc aaacgtaagt
     3901 acagagcaaa tgaaagtgtt aagtgacaat gagaaactga tggacgatct gggggcaaat
     3961 gtcacgccgg ctatctatta catgagtaag gaaaatacgc tgcaacaggc cgtggggttg
     4021 cccgatcaga aaacgcttaa tatcattatg ggaaataaat aaaaggtaat tcatggatgg
     4081 ttgaactata tccctgcttt tcaaccatcc taaaaaagca tgaatttctc gctctaccct
     4141 aagtggctgt gctattttag aaaaacttaa tcctgagtta agcaaaaaaa cataatcaat
     4201 aaaaatacat acgcaaaaaa cattgattaa gtgaatatac catggaagaa aaatataaac
     4261 cggagtagtg tatggccaat ctctacgact tgaaaaagtt cgacttaaat cttcttgtca
     4321 tatttgagtg tatttaccag catttaagca ttagcaaagc tgctgaatca ctatatatca
     4381 ccccatcagc cgtgagccag tcgttacaac gcttacgtgc gcaatttaat gaccctttat
     4441 tcattcgctc aggaaaaggt atcgcgccga cgacaacagg gttgaatctg catcaccatc
     4501 ttgagaaaaa cctcaaaggc cttgaacaaa caattaatat agtcaataag tccgaactaa
     4561 agaaaaactt tattatctac ggccctcagc tcatttcttg ctctaataat agtatgctga
     4621 tccgctgcct acggcaagat gcctctgtcg agattgagtg ccatgatatc ctcatgtcag
     4681 cagagaatgc tgaagaactg ttggttcacc gcaaagccga tctggtgatc acccaaatgc
     4741 cagtgatcag ccgttccgtc atttgcatgc cattgcatac aattcgcaat actctaattt
     4801 gtagtaataa gcatccgcga attactgaca atagcactta tgaacaaatt atggcagaag
     4861 agttcaccca attaatagcc aaatcagcag gagttgatga tattcaaatg gaaatagacg
     4921 agaagtttat gaatcgcaaa atttcatttc gtggttcctc tttgctgaca ataattaata
     4981 gcattgcagt tacagattta ctgggaatag tcccctatga actttataac taccaccgtg
     5041 actttcttaa tttaaaagag ataaaactag aacatccgtt accttcaata aagctttaca
     5101 tttcctataa taaatcttct ttaaataatt tggtgttctc ccgatttatt gaccgtctaa
     5161 atgagagttt ttgatatata actatagaca gctactataa ctttcatcta tttattcaca
     5221 gcgcgaatga aaaataaaac actctgcttt acagtgttta ctttataatt aacagtctta
     5281 tctcactgtt caccctgtaa cgttcagaga gcgtcttcag atgtctattt ataaaattcc
     5341 tcttccgctc aatattctgg aagctgcacg agagcgtatt acctagacac tcaacaccct
     5401 accccgcgtt tgtgtctcat tttctggcgg caaagactct ggtttaatgc tgcatctgac
     5461 agccgaactt gcccgacaaa tgggcaaaaa aatctgcgtt ttgtttatca actgggaggc
     5521 gc
//
LOCUS       DAZPEU010000223         5485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPEU010000223 DAZPEU010000000
VERSION     DAZPEU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.225"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1630)
                     /locus_tag="ACHU2L_003686"
     CDS             complement(<1..1630)
                     /locus_tag="ACHU2L_003686"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR1358354.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACHU2L_003687"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACHU2L_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACHU2L_003688"
     CDS             complement(2541..3146)
                     /locus_tag="ACHU2L_003688"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1358355.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="ACHU2L_003689"
     CDS             complement(3317..>5485)
                     /locus_tag="ACHU2L_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR1358356.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAZPEU010000224         5473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPEU010000224 DAZPEU010000000
VERSION     DAZPEU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.226"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHU2L_003690"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHU2L_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR1358357.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHU2L_003691"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHU2L_003691"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR1358358.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHU2L_003692"
     CDS             complement(3736..4092)
                     /locus_tag="ACHU2L_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1358359.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHU2L_003693"
     CDS             complement(4096..4512)
                     /locus_tag="ACHU2L_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR1358360.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHU2L_003694"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHU2L_003694"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR1358361.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1283 a         1449 c         1440 g         1301 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tat
//
LOCUS       DAZPEU010000225         5466 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPEU010000225 DAZPEU010000000
VERSION     DAZPEU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.227"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            144..452
                     /locus_tag="ACHU2L_003695"
     CDS             144..452
                     /locus_tag="ACHU2L_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR1358362.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(511..1605)
                     /gene="yaiW"
                     /locus_tag="ACHU2L_003696"
                     /pseudo
     CDS             complement(511..1605)
                     /gene="yaiW"
                     /locus_tag="ACHU2L_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
     gene            complement(1618..2838)
                     /gene="sbmA"
                     /locus_tag="ACHU2L_003697"
     CDS             complement(1618..2838)
                     /gene="sbmA"
                     /locus_tag="ACHU2L_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR1358363.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            3190..4347
                     /gene="ampH"
                     /locus_tag="ACHU2L_003698"
     CDS             3190..4347
                     /gene="ampH"
                     /locus_tag="ACHU2L_003698"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR1358364.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(4348..4851)
                     /locus_tag="ACHU2L_003699"
     CDS             complement(4348..4851)
                     /locus_tag="ACHU2L_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1358365.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(4939..>5466)
                     /locus_tag="ACHU2L_003700"
     CDS             complement(4939..>5466)
                     /locus_tag="ACHU2L_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1358366.1"
                     /translation="SLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGD
                     AGYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTP
                     YFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGD
                     VDQDWSANVGVKYTW"
BASE COUNT         1370 a         1424 c         1317 g         1355 t
ORIGIN      
        1 cacaacgaag attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat
       61 tgacaatcta aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa
      121 gcagatgact tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg
      181 gaaaatatcg caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt
      241 tttgcttttg ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg
      301 agcgtttaat gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg
      361 gcaccatttt cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc
      421 tatggctgca ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa
      481 cgccttatct ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc
      541 gtctacgcgc ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag
      601 ttgaatgcca ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg
      661 ataaactttc ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg
      721 acggcgaatt tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc
      781 ggttttcccg ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac
      841 gccgctggcc ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc
      901 gttaaaatca gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg
      961 gtaagtacca aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc
     1021 cattttccac ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg
     1081 cccaccggtg cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct
     1141 gatcagatcg tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc
     1201 gctataactt ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg
     1261 aatgtgcatc cgttcagcac gacggtcaat ctcttgctag gcgatcttgc ttaagcccgg
     1321 aacggccgga tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagtagac
     1381 gttttccagc gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca
     1441 ggcatcgcgg tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat
     1501 cgctttttcg ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag
     1561 aaccgccaac aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta
     1621 gctcaaggta tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg
     1681 cagacgtttg tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg
     1741 gaaagcaccg cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat
     1801 cgtaccggca acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa
     1861 atagagaatg cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt
     1921 ccgtacggcg ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc
     1981 accataaacc agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc
     2041 cggcagtttg atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc
     2101 ggcaatcacc agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga
     2161 gagcgttacc agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac
     2221 gcccatattc tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc
     2281 accttcgata tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg
     2341 ccagcggaac acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat
     2401 cagcgcaatc cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg
     2461 cggcgaactt agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc
     2521 gaccccgact tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga
     2581 ccagtattgc caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca
     2641 aacaatgtag taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat
     2701 cgggatctgc ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca
     2761 gaagataacg gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg
     2821 gaaaaaagac ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg
     2881 tattaaccgc atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata
     2941 gcaaggcgct tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca
     3001 aagattgttt acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc
     3061 aggaaaaaga tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc
     3121 cctttgcaat ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg
     3181 acaccaccgt tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc
     3241 tccgtccatg cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt
     3301 tatgccgatc atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac
     3361 ggtaaccagc gcgtctttcg tagttatggt gaaacgcgac ctggtaataa cgttcgcccg
     3421 cagctggatt cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg
     3481 gtgaaattgc tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg
     3541 ccaggcgcac gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc
     3601 cataccagcg ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc
     3661 tggccaacgc gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca
     3721 ggcagtcagg cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat
     3781 gcgtccggca agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg
     3841 aaagacacca cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc
     3901 ggtgccagtc cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg
     3961 cctggcgata tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt
     4021 aaccaggcag atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt
     4081 ggcatggatg tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg
     4141 aaagagggtc gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat
     4201 atggcgatga tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg
     4261 acgcgcttta aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat
     4321 aaaccgttgg ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac
     4381 cagtttgcct ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc
     4441 gacgacagaa cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga
     4501 tcgcaattct tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg
     4561 tagatgatat cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat
     4621 taaaccatca gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc
     4681 ttctctacgc agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc
     4741 actggaaaat ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa
     4801 acatttatcg agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc
     4861 ctttgcagta atgcccgtca aatccttgac gggcattatt tagattaaat taccagtatt
     4921 tcttcggagt gaagaatatt accaagtata tttaacaccc acgttcgcgg accagtcttg
     4981 atctacgtca ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa
     5041 gctgaactga gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga
     5101 atcaccgttc acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta
     5161 cggagtcaga gcctgatctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc
     5221 ataacgcatg ctgtcgtaag gctgaccgtc aactttcatg tcgttgctca gctggtagtc
     5281 atcaccagac tggaacagac cagaaatgct gccgtaagga gtcacgtaac cagcatcacc
     5341 cagtttgaag tcgtaaccgg ttttcaaacc gaagccccaa gcgtcggagt tggtgctacc
     5401 gtcaacgtaa gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct
     5461 caagct
//
LOCUS       DAZPEU010000226         5449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPEU010000226 DAZPEU010000000
VERSION     DAZPEU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.228"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHU2L_003701"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHU2L_003701"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR1358367.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHU2L_003702"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHU2L_003702"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR1358368.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="ACHU2L_003703"
     CDS             complement(3121..3420)
                     /locus_tag="ACHU2L_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR1358369.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="ACHU2L_003704"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="ACHU2L_003704"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR1358370.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHU2L_003705"
     CDS             complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHU2L_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR1358371.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..>5449)
                     /gene="yiaB"
                     /locus_tag="ACHU2L_003706"
     CDS             complement(5115..>5449)
                     /gene="yiaB"
                     /locus_tag="ACHU2L_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR1358372.1"
                     /translation="SKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTATFGN
                     YAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVCLFG
                     QMRLFRSV"
BASE COUNT         1301 a         1410 c         1353 g         1385 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgagg
//
LOCUS       DAZPEU010000227         5400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPEU010000227 DAZPEU010000000
VERSION     DAZPEU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.229"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..497
                     /locus_tag="ACHU2L_003707"
     CDS             <1..497
                     /locus_tag="ACHU2L_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR1358373.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHU2L_003708"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHU2L_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHU2L_003709"
     CDS             2500..3114
                     /locus_tag="ACHU2L_003709"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR1358374.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHU2L_003710"
     CDS             3114..3443
                     /locus_tag="ACHU2L_003710"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR1358375.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHU2L_003711"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHU2L_003711"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR1358376.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHU2L_003712"
     CDS             4529..4840
                     /locus_tag="ACHU2L_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1358377.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHU2L_003713"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHU2L_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1178 a         1344 c         1426 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggcaag
//
LOCUS       DAZPEU010000228         5394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPEU010000228 DAZPEU010000000
VERSION     DAZPEU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.230"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(84..719)
                     /gene="msrQ"
                     /locus_tag="ACHU2L_003714"
     CDS             complement(84..719)
                     /gene="msrQ"
                     /locus_tag="ACHU2L_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR1358378.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            complement(720..1724)
                     /gene="msrP"
                     /locus_tag="ACHU2L_003715"
                     /pseudo
     CDS             complement(720..1724)
                     /gene="msrP"
                     /locus_tag="ACHU2L_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            complement(1833..2246)
                     /gene="hiuH"
                     /locus_tag="ACHU2L_003716"
     CDS             complement(1833..2246)
                     /gene="hiuH"
                     /locus_tag="ACHU2L_003716"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR1358379.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2379..3050
                     /gene="hprR"
                     /locus_tag="ACHU2L_003717"
     CDS             2379..3050
                     /gene="hprR"
                     /locus_tag="ACHU2L_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR1358380.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3050..4408
                     /gene="hprS"
                     /locus_tag="ACHU2L_003718"
     CDS             3050..4408
                     /gene="hprS"
                     /locus_tag="ACHU2L_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR1358381.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(4516..5367)
                     /gene="hchA"
                     /locus_tag="ACHU2L_003719"
     CDS             complement(4516..5367)
                     /gene="hchA"
                     /locus_tag="ACHU2L_003719"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR1358382.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
BASE COUNT         1486 a         1168 c         1282 g         1458 t
ORIGIN      
        1 acttgatgcg atctgtgtgg gccggatagg gcgctcgcgc cgcatccggc cgggataacg
       61 cgaagtttgt cggcaatatg aagttataga gataatttgt tgtgggcctg cttgcgtaac
      121 cggttgaaaa gggaaagcaa cttcttatac cgtaaggcta aaagcagtac agccagcccg
      181 gcgtagatga gtggctgcgg tgaaataatc ttcactgacc acagataatg tattggggcc
      241 aggatcgcga caagatagac gaagttatgc aactgttgcc agtgtttgcc gagttttcgc
      301 tgcatcgact gggttgaagt gaacgctaaa gcaagcaaaa taacccagct gataattcct
      361 aacgttaaat aaggccgggt aattaactcc ttacctaata acgccagatt attcacgccc
      421 aactccagca atgcgtaact ggttaaatgc agtgtcgccc aggcaaagca ccataagcct
      481 aacaggcggc gagtgcgtat caataacggc tgttttgcgt agcgtgccag aggtgtgatt
      541 aacaaggccg caagcaaaaa tttcagtgca gtgcgaccgg taaaatgctg aatatctttc
      601 accggatcgg cacccagtcc accgtgattg atcgcccaga ccagccagag aaacggcaac
      661 aatccggcaa gatgcaggca aactttcagc catatcactt gttttgctgt cagacgcatt
      721 tagaaattct cccgcaaatc caggccacga tacagcgatg ccacctggtc ggcgtaacca
      781 ttaaacagta gtgttggctg gcgctgaaca tcgagaatgc cgcctgaacc aataaatcgt
      841 tcggtagcct gtgaccagcg cgggtgatca acatgcggat taacgttggc gtaaaaaccg
      901 tattcgtcag gcgctgccag attccaggtg gttggcggac gctcgcgggt cagcttaata
      961 ctgacgatcg atttaatccc tttaaagcca tatttccacg gcacaatcag tcgcaccggc
     1021 gcgccatttt gcggtggtaa cgccttgcca taaacaccta cggtcatcag tgtgagcgga
     1081 tgcattgctt cgtcgagacg caatccttcg acataaggat atttcagccc gccgccgata
     1141 aagcggtcct gctggccagg catctgttcc ggcgcataaa tcgtttcgaa agcgacatac
     1201 ttcgcattgc tggtgggttc ggcaagcgcc agcaatttgt gtagcggaaa accaatccac
     1261 ggcaccacca tcgaccacgc ttccacgcag cgcatacgat aaatacgctc ttccagcggg
     1321 aagcgacggg ttaaatcatc atgatcgagg gtcaatggtt ttgccacttc gccgctgatt
     1381 ttcagtgtcc atggatcggt tttcaggcta ccagcattag ccgcgggatc gtctttatcc
     1441 agcccgaatt catagaagtt gttataaccg gagactttat ctgctggcgt cagtggcagg
     1501 ttattttgct aggcggcagg cttgctgaac tccagcggtt ttccggcggg ggcgggcgga
     1561 cgatcgttcc ctttaaacca gctaagcaga tcggcatgcg cagcgtgagg caaagaaaat
     1621 gcagctgcgc tgatgcccag tgcttttaac acctgccgac gcttcataaa gaataccgac
     1681 tcggccgtaa catctgattc ttttaaaaat tgcttctttt tcataagcca tcacactttt
     1741 ttgtcgttaa ccaacaagag ctgtttactg ttaatctgtc agggagtcac ctccctaaca
     1801 gtagcagaga tcagctttta catatttacg cattaactgc cacgataggt tgaataccca
     1861 tattggctga gtaacaaagg cacatgataa tgctcgttca ctttattaat atgaaattca
     1921 acgggaatct cagggaagaa actttcaaga ttttgtttct tgaaatagtc cccggtttta
     1981 aatacgacac ggtaatcgcc agtagtcgca gtttgctcgg gccacagtgc cttaattcgt
     2041 ccatccttat ctgttttggc ggtattaagt tgtaaccagc cgttatccgc tttcttttca
     2101 agagtgactg tcacgtcggc agcgggtttt cctgtttgct ggttcaaaat gtgcacgcta
     2161 agaatgtttt gttgtgcggc ataaaccaaa gaaggtaatg aaaaggctgc cgttgctacc
     2221 gagagtacta aataacgctt taacatgttt atatccttgt catgtgaatg agttttgagg
     2281 atagcgaaaa aacgtccagg gtaaccttac agcagcatta caattttgta atgaaccagg
     2341 ttgtttctat aacatatgat ttatggcata ttattttcat gaagattcta cttattgaag
     2401 ataatcaaag gacccaggaa tgggtaacgc aggggctttc cgaagcgggt tatgtcattg
     2461 atgccgtttc tgatggcaga gatgggcttt atcttgcgct gaaggatgat tatgcattga
     2521 tcattctgga tattatgctt ccgggtatgg atggctggca gatcttacaa acgttaagaa
     2581 cggcaaagca aacccctgtt atttgcctta ctgcaaggga ttctgtcgat gacagagtca
     2641 gagggttgga cagtggggca aatgattatc tggtaaaacc tttttcattt tctgagttgc
     2701 tagcaagggt tcgggcacaa ttaaggcaac atcacgctct gaattcaaca ttagaaatca
     2761 gtggcttaaa aatggactct gtcagtcaaa gtgtcagcag ggacaatatc agtattacac
     2821 tgacgcgcaa ggagtttcag ttactttggc tactggcctc cagagctggc gagattatac
     2881 ccagaacggt tattgcgagt gaaatttggg gaatcaactt tgatagtgat accaatacgg
     2941 tggatgtcgc cattcgcagg ctccgcgcaa aagttgatga tccttttcct gaaaagctaa
     3001 tcgccacaat ccgggggatg ggctattcat tcgtagcggt aaaaaaataa tgaaaagatt
     3061 atctataacc gtccgtttaa ccttgctttt tatatttctg cagtctgttg ctggcgctgg
     3121 aattgtatgg actctctata atggcctggc aagtgagttg aaatggcgcg atgatacaac
     3181 actcattaac cggacagcgc agatcaagca gttgttaatt gatggggtaa atccagatac
     3241 gttacctgtg tactttaacc ggatgatgga tgttagtcag gatattttga tcattcatag
     3301 tgatggcatc aataaaattg ttaaccggac aaatgtcagt gatgacatgt taaataacat
     3361 acctgctagt gagacaatca gcgcagcagg catttacaga agcattatta atgatacaga
     3421 gatagatgct ttacgaatta acattgatga agtttcgcca tcattaacgg ttactgtggc
     3481 taaattggct tcagccagac ataatatgct tgaacagtat aaaattaata gcattataat
     3541 ttgcattgtc gccattatac tgtgctcagt attaagtccg ttgttaatca ggacggggtt
     3601 gcgagagatc aaaaagttga gtggcgtaac ggaagcgctg aattataacg atagccgagt
     3661 gccggttgag gttaacgcat taccgagaga actaaaacct cttgggcagg cgttgaataa
     3721 aatgcatcat gctttagtca aagattttga gcgccttagt caatttgctg acgatctcgc
     3781 tcatgaactt agaacgccaa ttaatgcatt actgggtcag aatcaggtta cgctcagtca
     3841 aatcagaagt atcgctgaat atcaaaaaac aattgccgga aacattgaag agctggaaaa
     3901 tatttcgcgg ttaacagaga atatactgtt tcttgccagg gcagacaaaa acaatgtttt
     3961 ggtgaaactg gattcgcttt ctctcaataa ggaagttgaa aatcttttgg actatcttga
     4021 atatctttca gacgagaaag agatttgctt taaggtcaag tgcaatcaac aaatctttgc
     4081 ggataaaatt ttactgcaac gaatgttatc gaatcttatt gttaacgcca ttcgatattc
     4141 accagaaaaa tcgcgtattc atataaccag ttttcttgat gccaacggct ctcttaatat
     4201 tgatatcgcc agtcctggaa cgaaaattaa tgagcctgaa aaactcttcc gtagattttg
     4261 gcggggagat aattcgcgtc attccgtagg tcagggactt ggcctttctt tagtcaaagc
     4321 gattgccgaa ttacatggag gaagtgctac gtatcactat ctcagtaagc ataacgtgtt
     4381 ccgaattacg ttaccgcaaa gaaattaaca tcgggcttca gtacgccata agttaaccgt
     4441 gaaaaaacgc agccacgtcg tgagtattaa cgtggctgcg attgattatg cgcttacatc
     4501 acaatgtaac agggattaac tcgcgtaagc tgccagcatt tcctgcgccg ccagtttacc
     4561 caacgcattc gctgcaaaag gactgtcgcc ggtgagacgt ttacggtcct tatgtactcg
     4621 cccggtgatg tcgtcattaa tgatgttcat gcccattttc ttcaactctt cgccgaagta
     4681 ccaggtgaga tgacccggca tatagccaat atctggcgtt tgtttgtctg cggcgtctgg
     4741 gaatgcgcaa atagaataac cattcagtgg gttatcgctg tggcgaagtg ccagaaaagc
     4801 cgccggaccg tggcaaaggg agataacaaa acggtcattt ttgattgccc actgtaaagc
     4861 ggcagccacg tcctggcttt ctggtaaacc aataagtgcg ccatgcccac caggaacaaa
     4921 gattgctgca tattcgctat cagcgttgag gctggcgaca acatccgcga gtttcttcgg
     4981 attacggaat aacgatttat gctgttcaaa gaatggcatt actttttcat ctttgtgcgg
     5041 catagcccag tattcaaact tggtcatcag accggaaatg gtcgccactt cgaattcgaa
     5101 acctgcagca tggagatgat acaacggcag caacgtttca atcggatggt taccggtcga
     5161 gaacagtttt ccgttatcgg tcggcaaata acgttcgtcc gcggcgatca ccagaatttt
     5221 atgtttaccg cgatacggtt ttggatagtc cacgccatca agatcagaga cgggactcgt
     5281 atattggcta agcgaatatt ctgaagggaa gaacgcatta tcttcagcaa tatcgacctg
     5341 cgggttttta cttgtttgaa cagtcatagt gtattcctta ttgttgctta gtta
//
LOCUS       DAZPEU010000229         5387 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPEU010000229 DAZPEU010000000
VERSION     DAZPEU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.231"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            91..2127
                     /gene="yihQ"
                     /locus_tag="ACHU2L_003720"
     CDS             91..2127
                     /gene="yihQ"
                     /locus_tag="ACHU2L_003720"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFR1358383.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            2173..3547
                     /locus_tag="ACHU2L_003721"
                     /pseudo
     CDS             2173..3547
                     /locus_tag="ACHU2L_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3590..4993
                     /locus_tag="ACHU2L_003722"
     CDS             3590..4993
                     /locus_tag="ACHU2L_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1358384.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            5061..>5387
                     /locus_tag="ACHU2L_003723"
     CDS             5061..>5387
                     /locus_tag="ACHU2L_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR1358385.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         1272 a         1324 c         1352 g         1439 t
ORIGIN      
        1 tttatatatc agttttatga gaatcatttt acataagcct gtaataacag gaaggcaaca
       61 atctcgttct caactctatg gagattagtt atggatacgc cacgtccaca gttattagat
      121 tttcaatttc atcagaataa cgacagtttt accctacatt ttcaacaacg tcttatttta
      181 acccataaca aagataatcc ttgtttatgg attggctcag gtatagcgga tatcgatatg
      241 ttccgcggta atttcagcat taaagataaa ctacaggaga aaattgcgct taccgacgcc
      301 atcgtcagcc agtcaccgga tggttggtta attcatttca gccgtggttc tgacattagc
      361 gccacgctga atatctctgc cgacgatcag ggccgtttat tgctggaact acaaaacgac
      421 aaccttaacc acaaccgtat ctggctgcgc cttgccgctc aaccagagga ccatatctac
      481 ggctgcggcg aacagttttc ctacttcgat ctgcgcggca aaccgttccc gctatggacc
      541 agtgaacaag gcgttggtcg caacaaacaa acctatgtca cctggcaggc cgactgcaaa
      601 gaaaatgcgg gcggcgacta ttactggact ttcttcccac agcctacgtt tgtcagcacg
      661 cagaagtatt actgccatgt tgataacagt tgctacatga acttcgactt tagcgccccg
      721 gaataccatg aactggcgct gtgggaagac aaagctacgc tacgttttga atgtgctgac
      781 acatacatct ccctgctgga aaaattaact gccctgctgg gacgccagcc agaactgccc
      841 gactggattt atgacggggt aacgctcggc attcagggcg ggacggaagt gtgccagaag
      901 aaactggaca ccatgcgtaa cgcgggcgtg aaggttaacg gcatctgggc gcaggactgg
      961 tctgggatcc gtataacctc ttttggcaag cgcgtgatgt ggaactggaa gtggaacagc
     1021 gaaaactacc cgcaactgga ttcacgcatt aagcagtggt atcaggaggg cgtgcagttc
     1081 ctggcctata tcaacccgta tgttgccagc gataaagatc tctgcgaaga agcggcacaa
     1141 cacggctatc tagcaaaaga tgcctctggc ggtgactatc tggtggagtt tggcgagttt
     1201 tacggcggcg ttgtcgatct cactaatcca gaagcctacg cctggttcaa ggaagtgatc
     1261 aaaaagaaca tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg
     1321 cccaccgaca cgtacctgca taacggcgtc agtgccgaaa ttatgcataa cgcctggcct
     1381 gcgctgtggg cgaagtgtaa ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc
     1441 ctcttcttta tgcgcgccgg ttctaccggt agccagaaat actccaccat gatgtgggcg
     1501 ggcgaccaga acgtggactg gagtctcgac gatggcctgg cgtccgtagt cccggcggcg
     1561 ctgtcgctgg caatgaccgg acatggtctg catcacagcg acattggcgg ttacaccacc
     1621 ctgtttgaga tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt cagcgccttc
     1681 acgccgatga tgcgcaccca cgaaggtaac cgtcctggcg acaactggca gtttgacggc
     1741 gatgcggaaa ctatcgccca ttttgcccgt atgactaccg ttttcaccac cctgaaacct
     1801 tatctgaaag aggccgtcgc gctgaatgcg aagtccggtc tgccggtcat gcgcccgctg
     1861 ttcctgcatt acgaagacga tgcacatacc tattccctga aatatcagta cctgttaggc
     1921 cgcgacattc tggtcgctcc ggtacacgaa gaaggtcgca gcgactggac gctctatctg
     1981 ccggaggata actgggtaca tgcctggacg ggtgaagcgt tccggggcgg ggaagttacc
     2041 gttaatgcgc ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga atgggcggca
     2101 ctgttcgcgt cgttaaacag catctaatca catccgcccg tggggaaact catgggcaaa
     2161 gggagaacat cgatgagtca catcacaacg gaagatccag caactttacg cctgcccttt
     2221 aaagagaaac tctcttacgg tattggcgac ctggcctcta acatcctgct ggatatcggt
     2281 acgctttatc ttttgaagtt ttataccgac gttctggggc tgccaggcac ctatggcggc
     2341 attatctttt tgatttcaaa attctttact gcgtttaccg atatgggtac cggcattatg
     2401 ttggattccc gacgcaagat cggtccaaaa ggtaagttcc gtccttttat tctgtatgcg
     2461 tcattcccgg tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt tgatgtcacc
     2521 ggtaaaacgg tgatggccac tattctgttt atgctttacg gactgttttt cagcatgatg
     2581 aactgctcct acggcgcaat ggtcccggca atcaccaaaa accccaacga acgcgcctca
     2641 ctggcggcat ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc
     2701 gtgccagtta tgaatcttat cgaaggtaat cagaaacttg gctatatctt cgccgccacg
     2761 ctgttttcac tgttcggcct gctgtttatg tggatctgct actcaggcgt gaaagagcgt
     2821 tatgtcgaaa cccaaccaac caatccggcg caaaagcctg gcctgttgca atctttccgc
     2881 gcaattgccg gtaaccgccc actgttcatt ctgtgtattg ccaacctctg caccttaggg
     2941 gcgtttaacg tcaagctcgc cattcaggtc tattacaccc agtacgtact taacgatccc
     3001 atcctgttgt cgtatatggg atttttcagc atgggctgta ttttcatcgg cgtgttcctg
     3061 atgcctggcg cagtcagacg ttttggtaag aagaaggtct atatcggcgg cctgctgatt
     3121 tgggtgctgg gcgatctgct caactatttc ttcggcggcg gctcggtcag ctttgtggcg
     3181 ttctcctgcc tggcgttctt cggctcagcg cctgaactgg gcgctggttt ccgacaccgt
     3241 cgagtacggc gagtggcgca ccggcgtgcg ttcggaaggc acggtctaca ccggcttcac
     3301 cttctttcgc aaagtgtctc aggcgctggc tggtttcttc cccggctgga tgctgacgca
     3361 aattggctat gtgccgaacg tcgcacaggc tgaccacacc attgaagggt tacgccagtt
     3421 gatcttcatc tacccaagcg cactggcggt agtcaccatt gtggcgatgg gttgcttcta
     3481 cagcctgaac gagaagatgt atgtccgcat tgtggaagag atagaagccc gtaaacgcac
     3541 ggcgtaataa taataaacga cgccctgcgg ggcgttataa ggagtgatta tgtctgacca
     3601 taatccactg acattaaaac tgaatctgcg ggaaaaaatc gcctatggta tgggcgacgt
     3661 cggttcgaat ttaatgctct gcatcggtac tctgtatctc ctcaaacttt ataccgatga
     3721 attagggatg cctgcttact atggcgggat tatctttctg gtcgcgaagt tttttaccgc
     3781 gtttaccgat atgctcaccg gatttttact cgactcgcgg aaaaatattg ggcctaaagg
     3841 caaatttcgt ccctttattt tatatgcagc agttccggcg gcgttaattg caacgctcca
     3901 gtttatcgcc accacctttt gtttgccggt taaaacgacg attgccacca cgctgtttat
     3961 gatgtttgga ctttcataca gtctgatgaa ctgctcgtat ggtgcgatga tcccggcaat
     4021 taccaaaaac ccgaatgagc gcgcgcagct cgcggcttac cgccagggtg gtgcaaccat
     4081 agggctattg atttgtaccg tggcgtttat tccgttgcag tcgctttttt ctgactcaac
     4141 cgtcggttat gcctgtgcgg cacttatgtt ctccattggc ggctttattt ttatgatgct
     4201 gtgctacaga ggcgtcaaag agcattatgt ggacacagcg ccaaccggac ataaagccag
     4261 tattctcaaa tctttttgcg cgatatttcg taatccacca ttgctggttt tatgcattgc
     4321 taacctgtgt actctggcgg catttaatat caaactggcg attcaggtct attacaccca
     4381 gtatgtgctg aatgatatta atttattgtc gtggatggga ttcttcagta tgggatgcat
     4441 cctcgtcggc gtattactgg tgccagtaac tgtaaaatgt tttggtaaaa aacaggttta
     4501 tttagctggc atggtgctat gggcggtggg tgatatactg aattatttct ggggaagtaa
     4561 ctctttcact ttcgtcatgt tctcttgtgt cgcctttttt ggcacggcct ttgtaaacag
     4621 cctgaactgg gcactggtac cagataccgt agattacggt gaatggaaaa ccggtattcg
     4681 tgccgaaggt tctgtttata ccggttatac cttctttcgt aaaatttctg ccgcacttgc
     4741 tggcttcttg ccaggcatta tgctgacgca aattggttat gttcccaaca tcgcgcaaag
     4801 cgatgcgaca ttacagggtt tgcgtcagct catttttatc tggccttgtg cactggcaat
     4861 tattgctgca ttgacgatgg gattctttta cacactcaat gaaaaacggt ttgcattgat
     4921 tattgaggaa atcaaccaac gcaaaaataa agagatggcg acagaaaaaa aaacggcttc
     4981 cgtaacgtta taaatgtcaa tagccgctat ttccatcctg gtgggtggcg gcctccctac
     5041 gtttaaaaaa tggacttatt atgaaaaaga ttaatgcaat aattttatta tcttctctga
     5101 cctctgcctc ggtatttgct ggcgcatacg tcgaaaacag ggaggcatat aatcttgcct
     5161 ccgatcaggg cgaggttatg ctacgtgtgg gttataactt cgatatgggc gcgggtatta
     5221 tgttaaccaa tacctacacc tttcagcgag aagatgaact aaaacatggc tataatgaaa
     5281 ttgaaggttg gtatccttta ttcaaaccaa ccgataaatt aaccatccag cccggtggct
     5341 taattaatga taagagtatc agttcgggtg gtgcagtgta tctggac
//
LOCUS       DAZPEU010000230         5374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPEU010000230 DAZPEU010000000
VERSION     DAZPEU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.232"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(99..581)
                     /locus_tag="ACHU2L_003724"
     CDS             complement(99..581)
                     /locus_tag="ACHU2L_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358386.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(649..2598)
                     /locus_tag="ACHU2L_003725"
     CDS             complement(649..2598)
                     /locus_tag="ACHU2L_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1358387.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2683..3018)
                     /locus_tag="ACHU2L_003726"
     CDS             complement(2683..3018)
                     /locus_tag="ACHU2L_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1358388.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(3018..3374)
                     /locus_tag="ACHU2L_003727"
     CDS             complement(3018..3374)
                     /locus_tag="ACHU2L_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1358389.1"
                     /translation="MAKIAGICFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3374..4870)
                     /locus_tag="ACHU2L_003728"
     CDS             complement(3374..4870)
                     /locus_tag="ACHU2L_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1358390.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4867..5028)
                     /locus_tag="ACHU2L_003729"
     CDS             complement(4867..5028)
                     /locus_tag="ACHU2L_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR1358391.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5038..>5374)
                     /locus_tag="ACHU2L_003730"
     CDS             complement(5038..>5374)
                     /locus_tag="ACHU2L_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358392.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1284 a         1565 c         1155 g         1370 t
ORIGIN      
        1 cagtaagttg gagtcattac caagaaagag aaggtaaaga aggtaagtcc atactgtctc
       61 cactataaaa aaaccgccaa tttctggcgg cttatatatt aatcatcatt aatatctttc
      121 atattcaatt tgcaattttc gtaggtcact cccgactcgc cattaacatt aactccagct
      181 ctgatattcc cgttttcttt tacatctaca aaaaactggc ctcttggcat cttgaacgca
      241 aatgtaaaac caacagtatc tgaatcaaaa tcatcatctt tatatattgg gatcaaaaat
      301 gtcatcgtcg ctggtgatgc attgtttgca aaatcaaata cacctatact tttgccgaat
      361 tcataagaag cttttctcaa tataatcgca ccgctggcag aaggggatgg gcacgatatc
      421 ctcagttgtc tgaatccatc tgtatccaag tcaacgtcat tatctgcaga tgttttaacg
      481 ttactaataa atcgtgaaac aatctcctca tttgttagta ttttcgcctg agcacagcaa
      541 gaggttaata ttgcaaccgt cattaaaaat ggctttatca caaaacaaac tccattgtta
      601 gcatgttacg cgataatttt gcctcatctt tcaccacaaa aaaagaactc accttttaaa
      661 tggagaatac ccaacatcat gggtgatttt cataaaggga tcgcctgttt tcggcaaatc
      721 gataacgcgc attccaggcg gcgcgttgtc aaacgtcacc ttaagttcgc tgcgggttgc
      781 cggtgaagat gcaaggctta acagctgatt cctttccagt gatacattcg gggtgtagcg
      841 tttatcggga gataaatacc catcaccacg tggcatctgc catccggtca tgtcatagac
      901 aaaatcatgg aactgtttgc cccactcgtc gaacttatca ttcagaccaa atccactgtt
      961 cagtgcatca gccacaaaat ttttaacaag ccagggatgt tctttttcaa actcctgcgc
     1021 catcatgccc agttcaagca atccaccaat cagactgatc tttccgaggc cctttaattt
     1081 cagccctgtt tttcctgccg ccgttgtcca ttgattctgg gcgactgttg ccgcacgaat
     1141 acttgccacc gccttcacac caatatacag ggcagaaacc gttgctattg tttttactgc
     1201 ctgttcccat ccccccattg ctctgacaac ctgatcaacc tcctgccaga cttttttcac
     1261 aaccggaccg accgtttccc agttatcaat tatgagatat gcaccaccga cgagaagcgc
     1321 aattaatccc tttgccggcg tcatattcat cacgctaccc atgatttttg ttatgcgtgt
     1381 caatgtgcca atcgccacgc ccatcgtcag taatgccgca ccagttttcg cgatggtttt
     1441 tacgacctca ggattttctc tgacaaacgt gcgcacttct tccagaaaag gcttcatctc
     1501 ttttatgcct tcattgagtg aaggcaggaa ggtttccccc agcgtggaag aaatcgcatt
     1561 gatctggttc tgcagcagca acaactgatt ttctgtcgtc gcggcacggg cggcatattc
     1621 tttctgcatt gagccgccat actgctgcgc atccgccaca cgattaaagt tggttcgcaa
     1681 caaatccaga ttcgtgagca gcggcgcaat ggcccccagg gattccttcc cgaacagcgc
     1741 tttcagcaca gcagcctgtt tttctttcgg cacttttgcc agagaatcca gtacgtgcag
     1801 catggccccg cgggcgtctt tctgcatatc cgccgccagt tttttcgggc taatgcgcaa
     1861 cgagcggagc acttttttct gcgatttggt ggcagaatcc ctcgctgtca gcgacaacat
     1921 aaaattcttt atccccgtcg cagcaatttc tgactccacc cccattccgg caatggttgc
     1981 ccccattgcg gcaatctctc cggaggccac acccgcaaca ctgcctaaag ggccaatacg
     2041 ggtcacaaca tcagaaatct ttttcgcact tgcaggaccg gtattgccaa gatagttaat
     2101 cttatccgca agccctgcca cctctccctg tgtcagttta aaggcagtgc gccactgcgc
     2161 catcatctga ccggattctt ctgccgtggt gtcaaaggcc acgcccatct tcacggcatc
     2221 gtcagtaaac tgcatcagct cgtcacgggc gataccagcc tgaccacccg ccgccacaat
     2281 ttcggcgata ccttccgccg acatgggcag ttctgttgac aggtcgcgca cctgctccgt
     2341 cattgcctta aacgcttccg gcgtatccag accatccacc actttccgga catccgccat
     2401 ttttgattca agggcaatgg cagatttgac cgggagcgcc agcgccccca gtactgcggt
     2461 tccggcaccc gctgcgccca gtgacaggct ggcaaactcc tttttaaaac ctttcagctg
     2521 gcgctgcatt cccttcagcg gtgccgatac cttatccacg gcagtgatga tcgccttcag
     2581 ctgaaaactg tcagccatgc ttcatctcct cgttaatgcg gacggcctcg gcttccagtt
     2641 ctgtaaactg cgaaacagcc acccgtcgca gctccagtgg attcagtttc cagaaccagg
     2701 caacattgta gagtcgtttc cggaggtgct tcccgtctcc gactgggtaa aaaaacgcag
     2761 gatctgcatg ctggtcttaa aaatatccag ttttgccatc tgcgccgccg atgaacacgg
     2821 gatccccgcc agcaacggga tatatttcag tgccacctgg ctgtccagtt taatactgcc
     2881 ttcaccggaa ataatgaaag gaaaacccag cgcctcgatt tcgtcatacg tgggctcgcg
     2941 caactccagc acatgcagca tttcgttatg cgctgtcacc ggttttttta aaactatttc
     3001 tgtaacgttc attgccatta ctgataccct ccctcttcgc cgtggaattc aagatctgcc
     3061 gtgccttctt cggcattatg attagcctct ccgtgcagcc atgccgccga aagcacatac
     3121 accatgccgt ttgccagctc agcggtaatc gtcatctggt ctgaggtggt aattttgtcg
     3181 accggaaagt ttttgggcac tttaaacgtg cccttaacgt aaggtgaccg ccaggtctcc
     3241 ttgtaatcca catcccctgc catgccgacg atgtcatccc ggacattggt gttcatcggc
     3301 acttcaatgc cacctgtcag cgataactgc tgaccatcca ctttaaaaaa acagattccg
     3361 gcgatctttg ccattatgct gactcctctg aatactgaag acggaactgg ttaaccaccg
     3421 caaagacacg taactgatta acataatccg gcgggaacag cgtattcagt cggttcgggt
     3481 tatctgcatc acgctcaact atcagatact gtttaaacag atcgtatttt tccacaatac
     3541 cggcacgctc catctgacga tatgtcgcca gaagttcccc tttgataacg gcaggagtga
     3601 caatcgcctg ccccggacca aaacgggtac catcatttgc cagcttgtga cgtccgtact
     3661 tgcttgtgat gaccgatttc agtttgcgaa gaacatatgc actggtatgc agagtttcac
     3721 tgtccagata gctgttgtct gccacgccat acgcattctt tttgtaggtg gttacggaac
     3781 gctggatctg caacgtgccg ccttccacat aagccgtcgc cacgccgtga gataaaaggg
     3841 tctgctgctc tgtcatgatg aatcgcttac ctttcggtgc cggaagcatc cccaccagct
     3901 cccccgtctg tgtcggacgg gcaggatcat tccggataaa taccgcttca cgggcaaggc
     3961 gactggcaac cagttcatcg acaggcgact gggtttcttt ttcgtaaccg gcaagcgtga
     4021 tatgttgctg attatgcata tctccggcac caaccagctc tgacagcgtt cccagttttg
     4081 cggtatagac atgcccgtat aactggcgcg cataactcca gcgaccgctg ctgtcattca
     4141 tttcggtcat catcatattg atggaggcgg catcgttgaa cggcagaccg ataaagtcga
     4201 atgcctcatc gcccatagca gcaacagcgg cggtaagatc aggcgctccg ctaccggcag
     4261 caccgacttc cgtcaccacc cgaagccctg ctggcaaaat ctcaccacca ccagaaccat
     4321 aataattcag acagaccggt aactcattac catacagccc cttatgacgg gcagtcagcg
     4381 tcaccacgcc tgcatcagat gacgccgtaa acggcagggt gataaccccg tttaccgctt
     4441 ccttaatcgc ggtggcaacc gcagtggcat catcgccatt caccacaggc acctgtacac
     4501 gggagcgccc gacatacaga ctcagggtgc cgctttcctc tgcttctccg gcaaccgtca
     4561 ccctgaccgt cgccgccgcc cctctggctt ccggtactgc aataacatac agttcaccga
     4621 aaggatctgt ctgacggtag acttccacca tacgcgccag ctggctcccc gccccacaaa
     4681 tctggcgggc ataatctgcc gatggcatca gcaccaggct gttaacctca atggctgcat
     4741 cgttgctggc atgcccgatc agtaatgcag gcgcgctggt caccgctgta tttgccgcag
     4801 aattgttcat ctcggcgtag aacaacggca ccagcgtatt cgacggaaca gtattaaaac
     4861 ttatcgtcat gattttttag ccttatcctg tttaacacgt accacatcac ccgccgctat
     4921 acggcggaac cagtagctgc tttcttccac atttcgccct tcggaaggca aaaggtctcc
     4981 acgggcaggg tcaggaaccg accgcccttt caggggtttc acaaacatga gggactctta
     5041 attctgaggg aaacgcattt ccagatggtg ctcaatgtca ccatccgggc cagtaccagg
     5101 atcgatgtaa tccacatcaa tactcagcag ggataaatcc ggcagggcat tcacgtcctc
     5161 tgcctgtcgc gtgtcttctt ccgtgatttc atacttcgcc gtaaaatcaa actggtaata
     5221 cagttcgtaa cggttcagat ccagcagggt gccaccgaca taaacaatct cgcccccttg
     5281 cgggtccggc atccatccaa gcagggcttt ccagagttct ctccggacat catgaacggc
     5341 gtcataggct gcccactgcc ctttttcatc acgc
//
LOCUS       DAZPEU010000231         5339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPEU010000231 DAZPEU010000000
VERSION     DAZPEU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.233"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHU2L_003731"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHU2L_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR1358393.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHU2L_003732"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHU2L_003732"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR1358394.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHU2L_003733"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHU2L_003733"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR1358395.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHU2L_003734"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHU2L_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR1358396.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1457 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAZPEU010000232         5325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPEU010000232 DAZPEU010000000
VERSION     DAZPEU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.234"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            17..544
                     /locus_tag="ACHU2L_003735"
     CDS             17..544
                     /locus_tag="ACHU2L_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR1358397.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            602..835
                     /locus_tag="ACHU2L_003736"
     CDS             602..835
                     /locus_tag="ACHU2L_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR1358398.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            896..2864
                     /locus_tag="ACHU2L_003737"
                     /pseudo
     CDS             896..2864
                     /locus_tag="ACHU2L_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            2933..3367
                     /gene="psiB"
                     /locus_tag="ACHU2L_003738"
     CDS             2933..3367
                     /gene="psiB"
                     /locus_tag="ACHU2L_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR1358399.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3364..4083
                     /locus_tag="ACHU2L_003739"
     CDS             3364..4083
                     /locus_tag="ACHU2L_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR1358400.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4305..4454
                     /gene="mok"
                     /locus_tag="ACHU2L_003740"
                     /pseudo
     CDS             4305..4454
                     /gene="mok"
                     /locus_tag="ACHU2L_003740"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4396..4521
                     /locus_tag="ACHU2L_003741"
     CDS             4396..4521
                     /locus_tag="ACHU2L_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFR1358401.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(4840..5136)
                     /locus_tag="ACHU2L_003742"
                     /pseudo
     CDS             complement(4840..5136)
                     /locus_tag="ACHU2L_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5137..>5325)
                     /locus_tag="ACHU2L_003743"
     CDS             complement(5137..>5325)
                     /locus_tag="ACHU2L_003743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358402.1"
                     /translation="HLFENLRRKGHHCHGLTHLSWAERKIFYRVLGKKFCGLFIYPGQ
                     SVRQVVAVYAPGDRGLNR"
BASE COUNT         1169 a         1433 c         1651 g         1072 t
ORIGIN      
        1 cagaaggaga cacatcatgg cagttcgtgg cattaacaag gtcattcttg tcggtcgtct
       61 gggaaaagat ccggaagtcc gttacatccc caacgggggc gcagtggcaa acctgcaggt
      121 ggccacgtca gaaacctggc gcgacaaaca gacgggggaa atgaaggagc agacggaatg
      181 gcaccgcgtg gtgctgttcg gcaagctcgc ggaagtggca ggtgaatatc tgcgtaaggg
      241 ggcgcaggtc tacattgaag gccagcttcg cacccgtagc tgggaagata acggtattac
      301 ccgttacgtc accgaaatcc ttgttaaaac cacgggcacc atgcagatgc tggggagtgc
      361 accacagcag aacgctcagg tgcaaccgca gcctcagcag aatggtcagt cacagagtgc
      421 tgacgcgacg aaaaaaggga gcgcgaaaac gaaaggccgt ggacgtaagg ctgcgcagcc
      481 ggagcctcag ccgcaaccac cggaggggga ggattacggg ttttcagacg atatcccgtt
      541 ctgactggcc tgactgtgat aaccgccccg tcctgcgcgg ggcatcaccg gagatatgag
      601 gatgagcgaa tatttcagaa tacttcaggg actgcctgac ggtcctttta cccgcaaaca
      661 ggccgaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgacc acggcgaaca
      721 gtttcgcctg gttgtccgta ataacggaac aatggtctgg cgtacctgga attttgagga
      781 cggtgccggg tactggatga accatgtcat ccgtgatttc gggatcctta agtaagagag
      841 gtgccggacg gggatcacgt ccggcagaca ttaactacac aataagggga tagtcatgtc
      901 agtaacaaag tctgaaccaa aatctacccg taaagcaagc cgtaaacctg caaaaacgca
      961 ggaaactgtc ctgtctgccc tgctggcgca gacggaggaa gtgagcgtgc cgctggcctc
     1021 gctgattaag tcaccactga atgtgcgcac ggtgccgtat tctgcggagt ccgtcagcga
     1081 actggctgat tccattaagg gggtcggcct gctgcagaat ctggttgttc atgccctgcc
     1141 aggtgaccgc cacggtgtcg ccgcaggtgg tcgccgactg gcagcactca acatgctggc
     1201 agagcgtggc atcattccgg ctgactggcc tgtccgcgtg aaaattattt cgcaggaact
     1261 ggcgacggcc gcatcgatga ccgagaacgg tcatcgtcgg gatatgcacc cagccgaaca
     1321 gattgccgga ttctgtgcaa tggcgcagga aggcaaaaca cctgcacaaa tcggtgattt
     1381 gctgggctat tcaccccgcc acgttcagcg aatgctgaaa ctggctgacc ttgcgcctgt
     1441 catcctcgat gcgctggcag aagacctcat caccaccgag cactgtcagg cgctggcgct
     1501 ggagaacgac accgcgcgtc aggtgcaggt gtttgaagcc gcctgtcagt cgggatgggg
     1561 cggtaaaccg gaagtacaga ccattcgtcg tctggtgacc gaaagtgaag tggcggtggc
     1621 agggaacagt aaattccgct tcgtgggggc tgatgccttc tcgccagatg aactgcgcac
     1681 tgacctgttc agcgacgacg agggtggcta tgtggactgc gtggcgctcg atgccgccct
     1741 gctggaaaaa ctccaggctg ttgctgaaca ccttcgggaa gccgaaggct ggggatggtg
     1801 cgccggacgc atggagcctg tcggtgagtg ccgtgaggat gccggaacat accgctgtct
     1861 gccggagccg gaagcggtgc tgacggaggc ggaagaagaa cgtctgaacg aactgatgac
     1921 gcgttacgac gcgctggaaa accagtgtga ggaatctgac ctgctggaag cagaaatgaa
     1981 gctgatgcgc tgcatggcga aggtcagggc gtggacgccg gagatgcgtg ccggaagcgg
     2041 tgtggtggtg tcctggcgtt atggcaacgt gtgtgtccag cgtggtgtgc agttgcgcag
     2101 cgaggatgat gcggctgacg acgctgaccg cacggaacag gtgcaggaga aagcgcccgt
     2161 ggaggaaatc agtctgccac tgctgacgaa aatgtcttcc gaacgcacgc tggcagtcca
     2221 ggcagcactg atgcagcaac cggacaaatc tctggcactg ctggcatgga cgctctgccc
     2281 tgatgaatgt gtttggcagc ggagcgtaca gtaaaccagc acaaatcagc ctggaatgta
     2341 aacattattc gctgaccagc gatgcgccat cggggaagga cggtgccgca ttcctggcgc
     2401 tgatggcaga aaaagcccgt cttgcagccc tgttaccgga gggatggtca cgggatatga
     2461 cgacattcct gtcactcagc caggaggtac tgttatccct gctcagtttc tgcaccgcgt
     2521 gcagcatcca cggcgttcag acccgcgagc atggtcacac gtcacgcagt ccgctggact
     2581 cgctggagag cgctatcgga ttccacatgc gcgactggtg gcagccgacg aaagcaaact
     2641 tcttcggaca cctgcaaaag ccgcagatta tcgatgccct gaatgatgcc ggactgtccg
     2701 gtgctgcgcg ggacgcggag aaaatgaaga aaggcgatgc cgctgaacat gcagagttcc
     2761 acatgaaaga caaccgctgg gtgcctggct ggatgtgtac accacgtcca caggcagaga
     2821 cggaaaccac ggagtaccgt gacgaccagg cagaagccgc ctgaccgcca gtcataaacc
     2881 atttatcacg ccgccctgcc ggagacgggg cggcagcaag ggagataccg tgatgaaaac
     2941 tgaactgacc ctgaatgtat tacagaccat gagcgcacag gaatatgaag atatccgggc
     3001 tgccggaagt gatgaacgtc gtgaactcac acatgccgtg atgcgggagc tggatgcgcc
     3061 ggataactgg acgatgaacg gcgagtatgg cagcgagttc ggcggctttt tccccgtcca
     3121 ggtccgtttc acgcctgccc acgaacgttt tcacctggcg ttatgttcac cgggcgatgt
     3181 ctctcaggtc tgggtgctgg ttctggtgaa tgccggtggt gagccgtttg cggtggttca
     3241 ggtacagcgg cggtttgcac cggaagccgt cagccattca ctggcactgg cggcgtcact
     3301 ggacgcgcag gggtacagcg tcagtgacat catccatatc ctgatggcgg aaggaggtca
     3361 ggcatgagcg cacgttcaca ggcactggtt cccctcagcg cagagcaaca ggccgcatgg
     3421 cgggcggtgg cggagacaga aaaacgccgc caccagggaa acacgctggc ggaatatccg
     3481 tatgcggggg cgtttttccg ctgtctgaac ggaagccgca ggatatcgct gtctgacctg
     3541 cgatttttca tgccgtccct gaccgcagaa gaactgcgcg gcaaccgttc tcagtggctg
     3601 tatgccgtcg atgtgctgat tgaaactcag ggcgaggtct gccttcttcc ccttcccggc
     3661 gatgcagcag agcggctgtt cccgtcggtg cggttccgtg tcagggagcg cagtcgccac
     3721 aaatccgcac tggtcatgca aaagtacagc cgccagcagg cgagggaggc agagcagaaa
     3781 gcccgtgcgt accaggcgct ggtcgctcag gctgaaatcg aactggcatt ccattcgccg
     3841 gaaacagtcg gaagctggca tgcacgctgg agtgaccggg tggcagagca tgacctggaa
     3901 accctgttct ggcagtgggg tgagcgtttt ccgtcactgg ccggaatgga acgctggcag
     3961 tggcaggata tgccgttctg gcaggttatt gcggaagcca gtcttgcagc aagagaggcc
     4021 ggtcacgctg tccgtgagat ggagcggtgg atggtgccga acaaactccg ggaggcggcg
     4081 tgatgcggca acaatcacac agattacccg taaacagcct gaatgagcgg gttattttca
     4141 ggaaaagtga gtgtggtcag cgtgcaggta tatgggctat gatgtgcccg gcgcttgagg
     4201 ctttctgcct catgacgtga aggtggtttg ttaccgtgtt gtgtggcaga aagaagatag
     4261 ccccgtagta agttaatttt cattaaccac cacgaggcat ccctatgtct agtccacatc
     4321 aggatagcct cttaccgcgc tttgcgcaag gagaagaagg ccatgaaact accacgaagt
     4381 tcccttgtct ggtgtgtgtt gatcgtgtgt ctcacactgt tgatattcac ttatctgaca
     4441 cgaaaatcgc tgtgcgagat tcgttacaga gacggacaca gggaggtggc ggctttcatg
     4501 gcttacgaat ccggtaagta gcaacctgga ggcgggcgca ggcccgcctt ttcagggctg
     4561 atgctggtct gactgcactg aagcgccttt ataaaggggc tgctggttcg ccggtggccc
     4621 ctttctcctt gctggcattg tacgggcatg aacaaccaga ctgccaggaa ggccgtttcc
     4681 ctccgggaac ggggatgaac gtgcgcctgc ggcgcccgtt cttttccccc gccttctctg
     4741 tttatgactt accagaaatc acatcacctt ccctttgatt attctctttt tcctcctgct
     4801 ctgactctga ttactgctct gactctgatt actgctgccc tgattattgt ctgttttgtt
     4861 gttctcctgc ttatgccttt tccgggcgtc cccctggcgg ggcgggctgt cgtgaacgga
     4921 gccagcgaag ctgtctccgc cctggcgggc ttccatccct gacgcaaaaa cccgggtttg
     4981 cagcgaccgg aacggtcgcg ccacccttgt cgcgccgtgg cctgcgctcc cggcatcccg
     5041 gcaaaagacg ccgggatgta aggagcaacg ccgtttcgca aaccgcgctt cacgtcgttg
     5101 cggcgggtcc ctgcccgttg ttctgtccgg tgccattcac ctgtttaagc ccctgtcgcc
     5161 gggggcatag acagccacaa cctgccgcac tgactgtcca gggtaaatga acagcccaca
     5221 aaatttttta cccaaaaccc ggtaaaaaat tttgcgttct gcccaggaca ggtgcgtcag
     5281 gccgtggcag tgatgcccct tgcggcgaag gttctcaaac aggtg
//
LOCUS       DAZPEU010000233         5310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPEU010000233 DAZPEU010000000
VERSION     DAZPEU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.235"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..356
                     /locus_tag="ACHU2L_003744"
     CDS             <1..356
                     /locus_tag="ACHU2L_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358403.1"
                     /translation="KPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSDHARLFGFT
                     AEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLNKMAESFGFTDNPRYIAEKN
                     YMEALLKKASPHTVR"
     gene            complement(325..2062)
                     /gene="aceK"
                     /locus_tag="ACHU2L_003745"
                     /pseudo
     CDS             complement(325..2062)
                     /gene="aceK"
                     /locus_tag="ACHU2L_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(2246..3550)
                     /gene="aceA"
                     /locus_tag="ACHU2L_003746"
     CDS             complement(2246..3550)
                     /gene="aceA"
                     /locus_tag="ACHU2L_003746"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR1358404.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3580..5181)
                     /gene="aceB"
                     /locus_tag="ACHU2L_003747"
                     /pseudo
     CDS             complement(3580..5181)
                     /gene="aceB"
                     /locus_tag="ACHU2L_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
BASE COUNT         1245 a         1422 c         1342 g         1301 t
ORIGIN      
        1 tgaagcccct cctcctggca aataattcta atgaatatcc cggtttgttt tcagcgatac
       61 agcataaaca acaaaacgtt gtagagacgg tttatcttgc tttatctgac catgcacgcc
      121 tgtttggatt taccgctgaa gatattatgg atttttggca acacaaagcg ccacaaaaat
      181 actctgcctt tgagttggct tttgaatttg gtcaccgggt tattgctgaa ttaatcctta
      241 atacattaaa taagatggct gaaagctttg gctttacgga taaccctcga tacattgcgg
      301 agaaaaatta tatggaagct ttactcaaaa aagcatctcc ccataccgta cgctaaatct
      361 ttgcctgcgc cgatacgcat aaacatcttc cacatgccct tcacgtatgc ggttttgtag
      421 tgcgcgccag taatcagcgc ggaacaggtc ggcgtgcatc tcttcaaaca gcggaccaat
      481 acgcgggtcg gcgcatagcc agtggcgaaa ctcttccggg aaaacatcgc ccggcgagac
      541 gctgtaccac ggttcgctgg caagttcgtc ttccggatag cgcggcggcg ggatgtcgcg
      601 gaaattcact tccgtcatgt agcaaatttc atcgtaatca taaaaaacca cacgcccgtg
      661 acgggtgaca ccgaagtttt taaacagcat gtcgccaggg aaaatgttag cagcggcaag
      721 ctggcgaata gcgttaccgt attcttcaat ggcgtcgcgc aactgctgac cttccacttg
      781 ttccagccag atattgagcg gcaccatccg ccgctcgatt atagagatgg cgaattacaa
      841 tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat tccattaata
      901 ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg tccgccattc
      961 ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga gcggcagaca
     1021 tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca aagcccggca
     1081 gcgtaaacac caacatcacc attccacgaa tacccggcgc ttcaatgaac tgctcattac
     1141 agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc ttctggcagc
     1201 cgatagccat atacaattca gcagtggttt tacctggcag aatttcccgt agccactcga
     1261 ccagcgctgc gggcagcggt gcataaacca taaaataaga acgcgcaaag ccaaaaacaa
     1321 tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg tcgtccgtct
     1381 ggtggatcgg cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg cccaccagcc
     1441 aggcggcttt attgcggtaa aacagttcgt tcgccacctg taaatgactt tccgcgaggt
     1501 tgtctgtccc cagcgtttcc gtcagatggc gaatgatgta atgaatgtca cggcttttat
     1561 tctgccagcg caggcgcagc ggcaggtcgc tgataacgcg catcagtaga gattcccagc
     1621 cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag cggcgctctg
     1681 gctgagagct aaagataaaa agccgctcgg gagtaagcga gcggtggtca aataaccgac
     1741 agtacacgga gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc ggcaacagcc
     1801 gcgtgtaatg ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg ccgttagtaa
     1861 tgcagcgcag ttgctccacg accagaccaa cgtgatgatc gtaaagatgg atacggtttt
     1921 tcatcgcctg ctggacagca tgccagtcgg cctgttcgaa acgctgctgc gccccggaag
     1981 tcacttcgag gaatcgacca tactgagcgt cgaagccttg caaaatggtt tgagcaatca
     2041 gtaattccag gccacgcggc atttagcgcc ctcatcagga gcagagaatt gccggatgcg
     2101 gcgtaaacgc cttatccagc ctacgttcgg caacggttgt aggcatgata agacgcgcaa
     2161 gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtaaatgcct tatccggcct
     2221 acagtcagca acgtttgtag gttgcttaga actgcgattc ttcagtggag ccagtcagcg
     2281 cggtgactga agacgtgccg ccctgaataa tagtcgtcac tttatcgaag taacctgtac
     2341 ccacttcctg ctggtgagat acgaaggtat agccatcttt cgcggcggca aattccggct
     2401 gctgcacttt ctcaacatag tgcttcatac cttcgccctg cgcataagcg tttgccaggt
     2461 cgaacatgtt aaaccacatg ctgtggatac ctgctagggt gatgaactgg aacttgtagc
     2521 ccatatccga cagctgctgc tggaagctgg caatagtttt gtcgtcgagg tttttctgcc
     2581 agttgaacga cggcgagcag ttataagcca gcagtttgcc cggatatttc gcgtggatag
     2641 cttgtgcaaa gcgacgcgcc agttccagat ccggcgtgga ggtttcacac cagaccaggt
     2701 cagcatatgg cgcatacgcc aggccacggc tgatcgcttg ctcaatgccc gcatgagtac
     2761 ggaagaagcc ttcactggta cgctcgccgg taataaattc gctgtcatac gggtcgcaat
     2821 ctgaggtgat cagatccgcc gcatcagcat cggtacgtgc aaccagcagg gttggaacgc
     2881 ccgtcacgtc agctgccaga cgcgccgcga ccagtttctg aatagcttcc tgagttggca
     2941 ctaaaacttt gccgcccatg tgaccgcatt tcttcactga cgccagctga tcttcgaagt
     3001 gaactgccgc agcaccggct tcaatcatcg ctttcatcag ttcaaaggca ttcaggacac
     3061 cgccaaaacc ggcttccgca tcggcaacga tcggcaggaa gtaatcgaca tagcgcggat
     3121 cgcccggctc aatgcccacg gaccattgga tctgatcggc acgacggaag gtgttgttga
     3181 tccgctccac cacagctggc accgagtttg ccggatagag cgactgatcc ggatacatgc
     3241 tggccgccag gttagcgtcc gccgctacct gccatcccga cagatagact gcttcaatac
     3301 ccgctttcgc ctgttgcagc gcctgaccgc cagtcagtgc accgaggttg ttgatgtagc
     3361 cttttttcga ctcaccgtgc agcagacgcc acattttcgc tgcgcccagt tgcgccagcg
     3421 tgcattcagg attgactgaa ccgcgtaatt tcaccacatc ttccgcactg tatgggcgag
     3481 taatgccttc ccaacgcggt tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac
     3541 gggttttcat gtgcagatgc tccatagtta tgtggtggtt tacgctaaca ggcggtagcc
     3601 tggcagggtc aggaaatcaa ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc
     3661 ggcatcgtca aaacggccct gggagaaacg ttcttcgccc agttcgctgg caatgacttt
     3721 catctcttcg cccagcatct ggcggaacaa ggctttggtc accggtttgc cattgctcaa
     3781 cgttttttga tgatggatcc actgccagat cgaggtacgg gaaatttcag ccgtcgccgc
     3841 atcttccatc aggccataaa tcggcacgca gccgttgccg gagatccacg cttcgatgta
     3901 ctgcacagcc acgcgaatgt tagcgcgcat accttcttcg gtgcgttcac catcacaagg
     3961 tgccagcagc tgatcggcag taatcggcgc gtcttgttcg cgcaatactt caagctgatt
     4021 tttacgggag ccgagaatgt cgttgaatac cgccattgcc gtgtccgcaa ggcctgggtg
     4081 agcgatccat gtgccatcgt gaccgttatt ggcttccagc gctttatccg cttttacttt
     4141 gttgagcacc tggttattac gctcttcatc tttgctcgga ataaacgcgg ccatgccgcc
     4201 catcgcaaaa gcaccgcgtt tatggcaggt tttaatcagc aggcgtgagt aagcattcag
     4261 gaatggttta tccatcgtca cggcctgtct gtctggcagg acacgatcgg gatagttttt
     4321 caacgttttg atatagctga agatgtaatc ccagcgaccg cagttcagac caacaatatg
     4381 gtcgcgcagc gcgtgaagga tttcgtccat ctggaacacg gcgggcagcg tttcaatcag
     4441 caacgtcgcc ttgatggtgc cgcgcggcag attaaagcga tcttctgcat agctgaagac
     4501 ttcgctccac caggccgctt cctgctagga ctgggttttc ggcagataga aatagggacc
     4561 gctgcccttt gccaacagtg cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag
     4621 gctaccgggg attgcctcac cacgccaggt gacatgtttt tccggcaagt gcagaccgcg
     4681 tacccgacaa atcaaaaccg ctggattggg cttgagctga taaattttgc ctgcttcatt
     4741 ggtatagctg atggtaccgt taaccgcatc gcgcaggtta atttgcccgt cgatcacttt
     4801 gttccagtct ggtgccagtg aatcttcgaa atcggccata aagactttca cattggcgtt
     4861 cagtgcgttg atcaccatct tgcgctctac cgggccagtt atctctacgc ggcggtcttc
     4921 taagtccgca ggaatcccgc gaattttcca atcagcatcg cgaatggaag ccgtttccga
     4981 aataaaatca ggcaacgttc cgttatcaat atcttgctgc tgctgaatgc gcgctgccag
     5041 aagtttattg cgttgtggcg taaaatgcgt caccagctca gtcagaaatt ctaccgcttc
     5101 ggcagtaaga atttgcttct cctgctcgcc atacggcctt gtgaaagcca gttcatcggt
     5161 tgttgttgcc tgttcagtca tcgtgcagct cctcgtcatg gatccgaaaa cttcccctct
     5221 gtgaacgaag gaacgttgtg cgcttttcgt tcagcacaac taagactact catttaaaat
     5281 gcaaaatcaa aaacaatttc cattttaatt
//
LOCUS       DAZPEU010000234         5281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPEU010000234 DAZPEU010000000
VERSION     DAZPEU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.236"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHU2L_003748"
     CDS             complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHU2L_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR1358405.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1957..2583
                     /locus_tag="ACHU2L_003749"
                     /pseudo
     CDS             1957..2583
                     /locus_tag="ACHU2L_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            2782..3870
                     /gene="serC"
                     /locus_tag="ACHU2L_003750"
     CDS             2782..3870
                     /gene="serC"
                     /locus_tag="ACHU2L_003750"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR1358406.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3941..5224
                     /gene="aroA"
                     /locus_tag="ACHU2L_003751"
     CDS             3941..5224
                     /gene="aroA"
                     /locus_tag="ACHU2L_003751"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR1358407.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         1313 a         1263 c         1358 g         1347 t
ORIGIN      
        1 taaaaaaacg ccccgtaata taactcagac taatcattaa gcctacattg cgtaggctat
       61 ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt aggccttcaa
      121 caacgactga tgtgcagcaa acgcgtgcag atcgctatct accggttgca ggccgtaaaa
      181 cgccgcttcg ccgctcattg ccgcactggc ggcttctacg gcatctgcgc cgtacatgcg
      241 aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgtgcca gtaataacaa
      301 cgtttgcagg cagcgataat agttggcgcg ttccggacta aatactgatg agttaaactc
      361 catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca gcgccagcat
      421 ggcttttaat tcaccgatac gcagggtgta ccaaccgtta tccgacccgg tcgccagacc
      481 caacagctca cgcacgcggg taaagtcatc aaaaccttct tcatccagtt gctcgatgag
      541 gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg tttcacgtaa
      601 atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa tatcggacat
      661 gccaggcacg ataatacggc aagcataaac gcccagatgc tcgtaatcgg caatataaac
      721 ttctttatct tctttgttga agatagccat cagcgtagca aactcttctt ccgtggtgcc
      781 ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt cccaggagat
      841 taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga cttcttcatc
      901 atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt gcagcagctc
      961 ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag aggcaaagca
     1021 ggtgccgttt gcaggattaa acagtaccac gcaaatcacc ggatactggc cgccaaggga
     1081 accatcatat gcgaagatcg ggaaaccttc tgtttccagt gtttcgatcg cttcaaccac
     1141 cgctgggtaa cgcgccagca cgtctgccgg gatttccggc aagctgatgc tttcagcaat
     1201 aatgcggttt ttcacgtagc gttcgaaaac ttcggacaac ccctgaacgc gtgcttcgtt
     1261 gcgggtattg ccagcggaca taccgttaga aacgtacagg ttaccaatga tattcatcgg
     1321 aatataaacg gtctgattgt cggactggcg cgtaaacggc agaccgcaaa taccacgatc
     1381 ttcgttaccg gattgtaggt caatcagcat actgccggtc aattcattct ccggatcgta
     1441 aaatgcgcgc agacggtcat cgagcagccc ttctggcaca tcgtcatttt cgctcagtgg
     1501 gaaccatttt tcgttgggat aatgcacaaa tggaccgttg gcgatggttt cgcccagcca
     1561 gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccga gtgcagaagc
     1621 cagcgccgct ttcttggttg cgcctttacc gttggtaaaa cacagtgcgc actctttgtc
     1681 gcgaatatgt acagaccaga cgttaggcac gggattcagc caggaggcct cttcaatctg
     1741 aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca gagcggcatc
     1801 tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga aagcgcgcaa
     1861 taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa ctgttgacta
     1921 tgcttttagc ggataaacca cgttaagagc ataaaaatga aggcgttcga tctccaccgt
     1981 atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg tagtctttat
     2041 acctttgtac tggttttttg ttcctcaaaa tgaccggaag acgcggtgtg cgacagatgt
     2101 cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat gtggcgtttt
     2161 atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg ttgttatacc
     2221 gcctggtaat gtgatggcga gctggcctgg tcgaaactca ataactccaa catgacggaa
     2281 tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca ggtacgtctg
     2341 gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga caaggtgaaa
     2401 ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt gccggagtcg
     2461 gcggactatg cctgcattcg ttgtagtgaa atcattcata tgaacgcggg ggaaaaacaa
     2521 ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa acgggtgacc
     2581 tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt gtgatgcaag
     2641 ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa tgataaaacc
     2701 gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg ggttaatagc
     2761 aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg tccggcaatg
     2821 ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa cggtcttggt
     2881 acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc agaggaagcc
     2941 gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt attctgccat
     3001 ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga taaaaccacc
     3061 gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc gaaaaaatac
     3121 tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc ggttaagcca
     3181 atgcgtgaat ggcaactctc tgataatgcg gcttatatgc attactgccc gaatgaaacc
     3241 atcgacggta tcgccatcga cgagacgcca gacttcggca aagatgtggt ggtcgccgcc
     3301 gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg tgtaatttac
     3361 gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt tcgtgaagat
     3421 ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat cctcaacgat
     3481 aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg tctggtcttt
     3541 aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca gcaaaaagca
     3601 gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt ggcgaaagct
     3661 aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga caaattgttc
     3721 cttgaagagt cttttgctgc aggccttcat gcactgaaag gacaccgtgt ggtcggcgga
     3781 atgcgcgcgt ctatttataa tgccatgccg ctggaaggcg ttaaagcgct gacagacttc
     3841 atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc cccacagcca
     3901 gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc tgacgttaca
     3961 acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg tttctaaccg
     4021 cgctttattg cttgcggcat tagcacacgg caaaacagta ttaaccaatc tgctggatag
     4081 cgacgacgtg cgccatatgc tgaatgcatt aacagcgtta gggttaagct atacgctttc
     4141 agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg cagaaggtgc
     4201 cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg cagctctttg
     4261 tctggatagc aatgatattg tgctgaccgg tgagccgcgt atgaaagagc gcccgattgg
     4321 tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg aacaagaaaa
     4381 ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg ttgatggctc
     4441 cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc cggaagatac
     4501 ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca cactcaatct
     4561 gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg tcgtaaaagg
     4621 cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat cttcggcttc
     4681 ttacttcctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg gtattggacg
     4741 taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg gcgcgaccat
     4801 ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta ttgatatgga
     4861 tatgaaccat attcctgatg cggcgatgac cattgccacg gcggcgttat ttgcaaaagg
     4921 caccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc gcctgtttgc
     4981 gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa gaggggcacg attacattcg
     5041 tatcactcct ccggaaaaac tgaactttgc cgagatcgcg acatacaatg atcaccggat
     5101 ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc ttgatcccaa
     5161 atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta gccaggcagc
     5221 ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt cggcacattg
     5281 t
//
LOCUS       DAZPEU010000235         5262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPEU010000235 DAZPEU010000000
VERSION     DAZPEU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.237"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..171
                     /locus_tag="ACHU2L_003752"
     CDS             <1..171
                     /locus_tag="ACHU2L_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1358408.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHU2L_003753"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHU2L_003753"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1358409.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHU2L_003754"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHU2L_003754"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR1358410.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHU2L_003755"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHU2L_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR1358411.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHU2L_003756"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHU2L_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR1358412.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1294 a         1183 c         1390 g         1395 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc ta
//
LOCUS       DAZPEU010000236         5162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPEU010000236 DAZPEU010000000
VERSION     DAZPEU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.238"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..220
                     /locus_tag="ACHU2L_003757"
     CDS             <1..220
                     /locus_tag="ACHU2L_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1358413.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="ACHU2L_003758"
     CDS             complement(392..721)
                     /locus_tag="ACHU2L_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR1358414.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="ACHU2L_003759"
     CDS             complement(726..911)
                     /locus_tag="ACHU2L_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR1358415.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="ACHU2L_003760"
     CDS             complement(908..3547)
                     /locus_tag="ACHU2L_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR1358416.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHU2L_003761"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHU2L_003761"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1358417.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5162
                     /gene="hslJ"
                     /locus_tag="ACHU2L_003762"
     CDS             4855..>5162
                     /gene="hslJ"
                     /locus_tag="ACHU2L_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR1358418.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1307 a         1322 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aa
//
LOCUS       DAZPEU010000237         5150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPEU010000237 DAZPEU010000000
VERSION     DAZPEU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.239"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            189..446
                     /locus_tag="ACHU2L_003763"
                     /pseudo
     CDS             189..446
                     /locus_tag="ACHU2L_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHU2L_003764"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHU2L_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR1358419.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHU2L_003765"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHU2L_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR1358420.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHU2L_003766"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHU2L_003766"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR1358421.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="ACHU2L_003767"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="ACHU2L_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3214..3430
                     /locus_tag="ACHU2L_003768"
                     /pseudo
     CDS             3214..3430
                     /locus_tag="ACHU2L_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3604..4419)
                     /locus_tag="ACHU2L_003769"
     CDS             complement(3604..4419)
                     /locus_tag="ACHU2L_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1358422.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5150
                     /locus_tag="ACHU2L_003770"
     CDS             4516..>5150
                     /locus_tag="ACHU2L_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1358423.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1175 c         1225 g         1376 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccct
//
LOCUS       DAZPEU010000238         5134 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPEU010000238 DAZPEU010000000
VERSION     DAZPEU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.240"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(453..1076)
                     /locus_tag="ACHU2L_003771"
     CDS             complement(453..1076)
                     /locus_tag="ACHU2L_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR1358424.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1373..1648
                     /gene="ypdI"
                     /locus_tag="ACHU2L_003772"
     CDS             1373..1648
                     /gene="ypdI"
                     /locus_tag="ACHU2L_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR1358425.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(1725..1967)
                     /locus_tag="ACHU2L_003773"
     CDS             complement(1725..1967)
                     /locus_tag="ACHU2L_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR1358426.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2320..3240
                     /gene="lpxP"
                     /locus_tag="ACHU2L_003774"
     CDS             2320..3240
                     /gene="lpxP"
                     /locus_tag="ACHU2L_003774"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR1358427.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3595..3666
                     /gene="ypdK"
                     /locus_tag="ACHU2L_003775"
     CDS             3595..3666
                     /gene="ypdK"
                     /locus_tag="ACHU2L_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR1358428.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3731..4969)
                     /gene="alaC"
                     /locus_tag="ACHU2L_003776"
     CDS             complement(3731..4969)
                     /gene="alaC"
                     /locus_tag="ACHU2L_003776"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR1358429.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
BASE COUNT         1353 a         1237 c         1147 g         1397 t
ORIGIN      
        1 cctctataaa taatctgcaa aatatcttcg tagcggcact cgatattttt aaattcctgt
       61 atcaagtact gaaaattatt ttatcgccac ttccaattcc tttctgcatg gtaagtagtg
      121 aggacgaaaa gaagaatatg ataaatatta attcgtggta atcagagaga gtcgcgacat
      181 ttgtgatgtt attcttacaa ctgtaagaag cgccggtgat ttacaagtaa gagtatgatt
      241 acgcttttta aagcgtacca taaagcataa cataataaat gatttgtgat gacacgggat
      301 tattgcggtt tactttgagg aaaataaaat gcgtgtttat agaaccgcga gcgccaaata
      361 attttatgac cacaacctgt cgtattgtgg tcataaaaaa ttgtcggctg gagggagtta
      421 aatcctttac ctcaatatac tgcctgtcag cattagttca caaacagtgc ttcgctatcg
      481 acgatgattc catcttccgc acctttaagc gccaggtttg cctcgtagta ttgcttttta
      541 tcaagcagtt tctgcgcatc agcaagtgca ttacgagtct gtttaagcgg catcagatat
      601 tggttttcca ttacgccaac accggccagg cgaagctctt ccattgcgcc ctttttatcc
      661 cctttcgcca ttttttcatt ggcaattttt atcgcggctt ctttttcggg tgttgctaca
      721 taactctcag aaattccgac agatgcatta atgacaatat attggtcatc attgagattg
      781 gtctttttac ctggcttagc gaactttgcc cattcggtac tgtcatctga cagcaaagcg
      841 gaagcttcat tggtcagttc ttttgccttt tccggatcgc catgaaacag agccaggcgc
      901 gctacctgca cgtcacgcat cgcatataaa ccttgttcag agatacgctc tgctgctgcc
      961 gttttctggg tttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat
     1021 acagttgaag atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt
     1081 acctcattct ttaacagttt tagaaaacgc cgccatcata ttgggtgagc atgaaatgag
     1141 aaatcagaga gcaacggtca ttttgaaagg gttatcttac aattgtagtt attatcgcaa
     1201 agcggcattt agtcttacag ttgtaggaat atgcctcctg tcgcagagaa ctattcttat
     1261 ataccacaaa gagatgtcat ttattatatt gatgtaaata ttgattaatt atatgtaaat
     1321 acagccataa catcgtatgg ttctcagtga accattttca ggataacttt ctatgaaagt
     1381 aaacttaata cttttcagct tatttttatt ggtctctatt atggcatgca atgtttttgc
     1441 attttccatt tctggtggtg taagtgagag gagctataaa gagactgaaa aaacatcagc
     1501 gatgacgacc acacactcta caaaacttca gccatcacag gcgattttgt ttaagatgag
     1561 agaagatgtg ccaccattaa acctcacaga agaaatcacg cctacttatc cgacaaaggc
     1621 gaattatctt attcatcctg tgcgatagcg tctgtcacat ggcaggtgaa caatcatgtc
     1681 tgatgctgat gtgaacacct gcccaaatga gccatcctga cccactatcg cctgccatac
     1741 cccaccataa agcgaatcat caagccacaa gccggggcaa gaaacataaa tatccccgtc
     1801 accagttcat taccgccagt taaccacatg gtgaagtaat aaatgagtgc agcgaggatc
     1861 accatgccga agaaagaaga gaccgccgat accacgttca gtacctgacc gatgtagccg
     1921 cttacgcaga caatacacag ggcgagaaac atccataaat aaatcatagc tcaacctcct
     1981 gccgcgctaa aaaacctgta acttcagtca ctttaatagt aagacaatcc ttaacctctg
     2041 gcaatttttg ctaagcatca atgaaaacag cacgttaaat gcaacgctat tgcgtgcgga
     2101 aaatttttta ctttgcacga ttaataatca gtagctgaaa gcagtcagcc agtgataaag
     2161 tagagccacc gcattcatgc tcctcgtggt tatgtcctga ctagtcttta cactctttac
     2221 aggaaccatt gtcgtacatg atggcccaac caattgagtg taccgctgtc gacatggaat
     2281 cttctgcgat acaacaattc gtatctacag aaggtaacta tgtttccaca atgcaaattt
     2341 tcccgcgagt ttctacatcc tcgctactgg ctcacatggt ttgggcttgg tgtactctgg
     2401 ctttgggtac agcttcctta tcctgttctc tgctttctcg gcacgcgtat tggcgcaatg
     2461 gcgcgaccat tcctgaaacg tcgtgaatct atcgcccgta aaaacctgga actttgtttc
     2521 ccgcagcatt ctgcggaaga acgcgagaag atgattgccg aaaactttcg ttcactcggc
     2581 atggcgctgg tagaaaccgg catggcatgg ttctggcccg acagtcgcgt acgtaaatgg
     2641 tttgatgttg aagggttgga taaccttaaa cgcgcacaaa tgcaaaatcg cggcgtaatg
     2701 gttgtcggcg tccattttat gtcgctggaa ctgggcggcc gcgtgatggg actgtgccaa
     2761 ccaatgatgg ctacctatcg tccacataat aatcagctga tggaatgggt gcagacccgt
     2821 gggcgcatgc gctctaacaa agcgatgatc ggcagaaata atctgcgcgg cattgtcggt
     2881 gcactgaaga aaggtgaagc ggtatggttt gctcccgatc aggattatgg tcgtaaaggc
     2941 agctccttcg cgccgttctt tgcggtggaa aatgtcgcca caaccaatgg cacctatgtt
     3001 ctctcccgtc tctctggcgc agccatgttg accgtaacga tggtaagaaa agcggattac
     3061 agcggatatc gtttgttcat caccccagag atggaaggct acccgacaga tgaaaatcaa
     3121 gccgctgctt atatgaacaa gattatcgag aaagagatca tgcgcgcacc ggagcagtac
     3181 ctctggatcc accgtcgctt taaaacgcgc ccggtgggag aatcgtcgtt gtacatttaa
     3241 caaactgctc ttactctttc cctcctcaaa tcggccataa taacctcatg atttttatag
     3301 cttttttctt aagttttttc cttcgctgaa tatctaatta ttaccgtcag aaattaaact
     3361 gtcgccgata tgagacactt gtaagttact aaaagtaaat aagaaaatgc ctcttgtctc
     3421 gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt ttagctcaga attctggggt
     3481 gatgcccacg gtaaaataca gggcttttaa ggtcgctttg cccggtgtca actcactgta
     3541 tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg cggagagatg taaagtgaaa
     3601 tatttcttta tgggcatttc ttttatggtc atcgtttggg ccggtacttt tgccctgatg
     3661 atctaaaagc aaactgtgca atgaaaaaca ggagccatca ggctcctgtt tgcatttctg
     3721 acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct gctgacgggt agtaaaccgt
     3781 cggcacggaa catcgcttta atccctctga tggcctgacg aatacggtcg tggttttcaa
     3841 tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa gccaatcccc ggcgagacac
     3901 aaaccttcgc ttcgttaagg agcttcttgg caaattccag cgatcccatg gccgcatatg
     3961 gttccgggat tttcgcccag acatacatcg aagccttcgg catttcgacc atccagcccg
     4021 cttcatgcag ccctttaacc aatacatcac gacggcgttt gtactgttcg gcaatgtcgc
     4081 gcacgcactg ttgatcgccc tcgagtgccg caatcgccgc cacctgcaac ggcgtaaaag
     4141 tgccgtaatc gtgatagctt ttgattcgcg ccagggcact gaccagcgtt ttgttgccga
     4201 ccataaagcc gatacgccag cccgccatgt tgtagctttt cgacagcgta aagaactcga
     4261 ccgccacatc gcgtgcaccc ggcacctgca taattgacgg cgctttccag ccatcgtaga
     4321 cgatatcggc ataggccagg tcatggacca ccagcacatc gtagcatttc gccagcgcca
     4381 caaccttttc gaagaactcc agttccacgc attgcgcggt tgggttcgac gggaagccga
     4441 ggatcatcat cttcggtttc ggataacttt cacgaatggc gcgctccagt tcgttgaaga
     4501 aatcgacacc ttccaccagc ggcactgagc gtacctgcgc cccggcaatc accgcgccat
     4561 aaatatggat cgggtaactt ggattcggca ccagcaccgt gtcaccatga tccagcgtcg
     4621 ccagcatcag atgcgccagg ccctctttcg aaccaatagt gacgatggct tctgattccg
     4681 ggtcgatttc aacgtcgtag cgatcctgat accagcggga aatggcgcga cgtaaccgcg
     4741 gaatgccgcg tgaagtggag taaccatgcg tgtccgggcg ctgggccaca gtacataatt
     4801 tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc catgctgaaa tcgataatat
     4861 cttcgccgcg ccgacgcgca gccattttca gttcagcggt gatgttaaaa acgtagggcg
     4921 ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt gtcagccata atttcctcag
     4981 attaacgtta gcgcccggac cgtccgagcg acgctgccac aatggtggct gttttgaaaa
     5041 tagcctgatt aatttctgtc tgtccagcgt tttagaacat ttttatcaat ggatttaaat
     5101 gaggaactcc acttcaaaaa caggaaattt ttct
//
LOCUS       DAZPEU010000239         5092 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPEU010000239 DAZPEU010000000
VERSION     DAZPEU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.241"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            460..1845
                     /gene="dcuC"
                     /locus_tag="ACHU2L_003777"
     CDS             460..1845
                     /gene="dcuC"
                     /locus_tag="ACHU2L_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1358430.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1886..2227)
                     /locus_tag="ACHU2L_003778"
                     /pseudo
     CDS             complement(1886..2227)
                     /locus_tag="ACHU2L_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2565..3074)
                     /locus_tag="ACHU2L_003779"
     CDS             complement(2565..3074)
                     /locus_tag="ACHU2L_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1358431.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            3246..3482
                     /locus_tag="ACHU2L_003780"
     CDS             3246..3482
                     /locus_tag="ACHU2L_003780"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1358432.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            3457..5088
                     /locus_tag="ACHU2L_003781"
                     /pseudo
     CDS             3457..5088
                     /locus_tag="ACHU2L_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1292 a         1117 c         1250 g         1433 t
ORIGIN      
        1 aaatatttcc gcgcatcgat taaagatgat gcgtcgtttt ctgcgatggg aatagtcaaa
       61 aaagaaaaac caagtctttt ttgatgacaa aatgcaatca aggaaaaatt attttatttt
      121 tttaacgaga tacaacaatc atcttaacga agtatataat atatatcgtc aacccatgct
      181 catattagat tattttttga gcgcacagcc gcagctaaca ccattgcaat taacaaattt
      241 gcatcaatcc accatcaatt tacacacatt attatgtgat aattgccaac cgctaaatat
      301 gcgttttgtt atctatgtat aaaaacagca acttcaatgt cttaatggca gtttttcttg
      361 attttaatca gcattcatcg ccaatttatt gggcatattt tttccttaag ctttaggaag
      421 tttttattat ttactttggg cctggagaca ggaaaaatta tgctgacatt cattgaactc
      481 cttattgggg ttgtggttat tgtgggtgtg gctcgctaca tcattaaagg gtattccgcc
      541 actggtgtgt tatttgtcgg tggcctgtta ttgctgatta tcagtgccat tatggggcac
      601 aaagtgttac cgtccagcca ggcttcaaca ggctacagcg ccacggatat cgttgaatac
      661 gttaaaatat tactaatgag ccgcggcggc gacctcggca tgatgattat gatgctgtgt
      721 ggctttgccg cttacatgac ccatatcggc gcgaatgata tggtggtcaa gctggcgtca
      781 aaaccattgc agtatattaa ctccccttac ctgctgatga ttgccgccta ttttgttgct
      841 tgtctgatgt cactggccgt ctcttccgca accggtctgg gggttttgct gatggcaacc
      901 ctgtttccgg tgatggtaaa cgttggtatc agtcgtggcg cagcagctgc catttgtgcc
      961 tccccggcgg cgattattct cgcaccgact tcaggggatg tggtgctggc ggcgcaggct
     1021 tccgaaatgt cgctgattga cttcgccttc aaaacgacgc tgcctatctc aattgctgca
     1081 attatcggca tggcgatcgc ccacttcttc tggcaacgtt atctggataa aaaagagcac
     1141 atctctcatg aaatgttaga tgtcagtgaa atcaccacca ctgcccccgc gttttatgcc
     1201 attttgccgt tcacgccgat catcggtgta ctgatttttg acggtaaatg gggtccgcaa
     1261 ttacacatca tcactattct ggtgatttgt atgctgattg cctccattct ggagttcctc
     1321 cgcagcttta atacccagaa agttttctct ggtctggaag tggcttatcg cgggatggca
     1381 gatgcgtttg ctaacgtggt gatgctgctg gttgccgctg gggtattcgc tcaggggctt
     1441 agcaccatcg gctttattca aagtctgatt tctatcgcta cctcgtttgg ttcggcgagt
     1501 atcatcctga tgctggtatt ggtgattctg accatgctgg cggcagtcac gaccggttca
     1561 ggcaatgcgc cgttttatgc gtttgttgag atgatcccga aactggcgca ctcttccggc
     1621 attaacccgg cgtatttgac tattccgatg ctgcaggcgt caaacctggg tcgtacccta
     1681 tcacccgttt ctggcgtagt cgttgcggtt gccgggatgg cgaaaatctc accatttgaa
     1741 gtcgtaaaac gcacctcggt gccggtgctt gttgggctag tgattgttat cgttgctaca
     1801 gagctgatgg tgccaggaac ggcagcagcg gtcacaggca agtaaatagt aatgccggag
     1861 gcaagtttcc tccggcttta tcatgtcacc cactgtggta tatgcgttgt ggtctgccaa
     1921 ctttgccgtg aacaatttca gcaataatca gatggcggct ggcgcaatat tcaagataac
     1981 gcctggcagt ggtgcggctg atggtcagtg cctgcgccac cgtttctgcc gtatgttgca
     2041 caccaggctc tttaaacagt tttcgcaccg cgtttagcgt caaggggtca atgccggtcg
     2101 gcagttcgtc tttgggttca ccgcgagcat aggcattaaa catctcatca atttgcttct
     2161 ggctggcgct atcaatactt tccagcatat gtttacgctg gcggaaacgg gttagcgttt
     2221 gccccagtgt attgattcac ttgaagtacg aaaaaaccgg gagaacattg gattattcgg
     2281 gatctgatgg gattagattt ggtggggctt gcaagcctgt agtacaaatt ttaggcgtta
     2341 accaatgaaa cgcgaaagat agtaaaaaat tgcttttgtt tcattgaaaa aacgaaaaac
     2401 aaaaacacta taaatcattt caatcatagc ttcaaaaaac gttcaaaacc gataacaacc
     2461 aggctgtcat caaatgactc atatcacaaa tcagctcatg ccgtttaggt atgttacatg
     2521 tgtgattatg tgaggtgaag tatgttttag ctggctcatg gttgttatac ggcttttttc
     2581 gcatcttgtg gttccaacaa aggagctaca ggctcttcct gtttatgaac accatacatt
     2641 gacaaaatct cttggcgagt ttctggaggt aatttatgca gcgccaaagc tgtttgtagt
     2701 gtttggcgat caatacccag cgttgccaat agggtgtcaa cggagatgga ggggcgttct
     2761 tgcattaatc cggaaagccc atatttagcc atgtttagct ctatctctcg gacttcattt
     2821 tctcctagtt tattgattaa cagggagaac attttcaatg tatcagcatt catttcttcg
     2881 atagcaattt tttgcatagc cggtattgcc gggatatatt caaccccagg cccgcgacgc
     2941 ccttcttgtg aacgcttcgg ccatccttct ttctttgctc gtttatgcac cccctgtatt
     3001 gtcccaggca ttccatccag ttcagtaatt tgtttgggtg tcatccactg gtttatctca
     3061 tttgcgcttt tcataaacta agtttaccag atttatctat ataaaaatca atggattaca
     3121 aaataaacca cattaactga tataaaccgg tttggtttat ttggtaatgt ataaaacaga
     3181 taatcaagac gaattatttg ttttggtaac cctttatcat acgcaatatg gttggtgtgc
     3241 gaaagatgag taacgattgg catcgggccg acgtgattgc ggccctcaaa aaaacaggaa
     3301 cccctctggc aaaattgtcc agacgttcag gttattcaac cgggacgctt gcaaatgcat
     3361 tagtcaggcc atggcctaaa ggtgaacgca ttattgctga cgccttaaac atgaaacctg
     3421 aggaaatttg gcctagtaga tatctaaggg atcacaatga gagagtggat atcagcaatt
     3481 gaatgcatgg gtttatctgg tttacctggt tcattaatgg gggttcacaa aagagctcag
     3541 agagaaggat ggctcaagag aagaagagaa ggaattagag gggttgctta cgaataccat
     3601 atatcttctc ttcctaacat tgctcgagct gaagttttgc tccaacaagg aaaatttaaa
     3661 accagtcagg gatgttttga aatcgcccgc cccacgctgg aagctcatga ttatgatcgt
     3721 gaggcactat ggagcaaatg ggataaagcc agcgattccc agcgcagcct ggctgaaaaa
     3781 tggttgccct caattcaggc cacagacgaa atgctgaacc aggggatttc aacgaaaacg
     3841 gcttttgcga ccgttgcagg gcattaccag gtcagcgcat ccactttgcg ggacaagtat
     3901 taccaggtac agaagtttgc gaagcctgac tgggcggctg cacttgttga tggacgtgga
     3961 gcatcccgtc gcaatgttca caaaagtgaa tttgacgagg atgcctggca gtttctgatt
     4021 gcagattatc tgcgaccgga aaaacccgcc ttccgcaaat gttatgagcg tctggaactg
     4081 gcagcccgcg agcatggctg gagtattccc tcccgtgcca cggcctttcg ccggattcag
     4141 caactggacg aggcaatggt tgttgcctgt cgtgaaggtg aacatgcact gatgcatctg
     4201 ataccggcac agcagcgaac tgtggaacac ctggacgcca tgcagtggat caacggcgac
     4261 ggttatctgc ataacgtctt tgtacgctgg tttaacggtg atgtgatccg tccgaaaaca
     4321 tggttctggc aggatgtgaa aacccgaaaa attctgggct ggcgctgcga tgtgagcgag
     4381 aacattgatt caattcgcct ctcgttcatg gatgttgtga ctcgctacag tatcccggag
     4441 gattttcaca tcaccattga taacacccgt ggtgctgcga ataaatggct gacgggaggc
     4501 gcgcccaatc gctaccgctt taaggtaaaa gaggacgatc caaaaggact gtttttactg
     4561 atgggtgcga aaatgcactg gacaagcgtt gttgccggta aaggctgggg ccaggcaaaa
     4621 cctgttgaac gtgctttcgg tgttggtggg cttgaggaat acgttgataa gcatccggca
     4681 ctggctggcg catatacggg gccaaatccg caggcaaaac ctgataacta tggcgaccac
     4741 gctgttgatg cagagctgtt tctgaaaacc tttgccgaag gtgtggcgat gttcaatgcc
     4801 agaacaggcc gtgaaacaga aatgtgcggg ggcaaactct cgtttgatga tgttttcgag
     4861 cgtgaatacg ccagaacgat tgtgcgtaag ccaaccgaag aacaaaaacg gatgctgtta
     4921 ctgcctgccg aggcggtgaa cgtttcacgc aaaggcgagt ttacgcttaa agttggcggc
     4981 tcccttaaag gcgcgaaaaa cgtttattac aacatggcat taatgaatgc cggagtgaaa
     5041 aaagttgtgg tcagatttga tccacagcag ttacacagca cggtttattg ag
//
LOCUS       DAZPEU010000240         5073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPEU010000240 DAZPEU010000000
VERSION     DAZPEU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.242"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..244
                     /locus_tag="ACHU2L_003782"
     CDS             <1..244
                     /locus_tag="ACHU2L_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358433.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHU2L_003783"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHU2L_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHU2L_003784"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHU2L_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR1358434.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHU2L_003785"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHU2L_003785"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR1358435.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHU2L_003786"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHU2L_003786"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR1358436.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHU2L_003787"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHU2L_003787"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR1358437.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1150 a         1293 c         1459 g         1171 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gag
//
LOCUS       DAZPEU010000241         5068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPEU010000241 DAZPEU010000000
VERSION     DAZPEU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.243"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(11..322)
                     /locus_tag="ACHU2L_003788"
     CDS             complement(11..322)
                     /locus_tag="ACHU2L_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR1358438.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACHU2L_003789"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHU2L_003789"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACHU2L_003790"
     CDS             701..1054
                     /locus_tag="ACHU2L_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR1358439.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHU2L_003791"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHU2L_003791"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR1358440.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACHU2L_003792"
     CDS             complement(2810..3280)
                     /locus_tag="ACHU2L_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR1358441.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHU2L_003793"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHU2L_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACHU2L_003794"
     CDS             complement(4119..4337)
                     /locus_tag="ACHU2L_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR1358442.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHU2L_003795"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHU2L_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR1358443.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         1364 a         1173 c         1216 g         1315 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaat
//
LOCUS       DAZPEU010000242         4973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPEU010000242 DAZPEU010000000
VERSION     DAZPEU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.244"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHU2L_003796"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHU2L_003796"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR1358444.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHU2L_003797"
     CDS             1020..1703
                     /locus_tag="ACHU2L_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR1358445.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHU2L_003798"
     CDS             1700..2161
                     /locus_tag="ACHU2L_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR1358446.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHU2L_003799"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHU2L_003799"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1358447.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHU2L_003800"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHU2L_003800"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR1358448.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHU2L_003801"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHU2L_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR1358449.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHU2L_003802"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHU2L_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR1358450.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHU2L_003803"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHU2L_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358451.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1177 a         1305 c         1238 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZPEU010000243         4870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPEU010000243 DAZPEU010000000
VERSION     DAZPEU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.245"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHU2L_003804"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHU2L_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR1358452.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHU2L_003805"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHU2L_003805"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR1358453.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHU2L_003806"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHU2L_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHU2L_003807"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHU2L_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR1358454.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHU2L_003808"
     CDS             4169..4342
                     /locus_tag="ACHU2L_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358455.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHU2L_003809"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHU2L_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR1358456.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHU2L_003810"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHU2L_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR1358457.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHU2L_003811"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHU2L_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZPEU010000244         4793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPEU010000244 DAZPEU010000000
VERSION     DAZPEU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.246"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(277..1134)
                     /gene="sitD"
                     /locus_tag="ACHU2L_003812"
     CDS             complement(277..1134)
                     /gene="sitD"
                     /locus_tag="ACHU2L_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR1358458.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1131..1988)
                     /gene="sitC"
                     /locus_tag="ACHU2L_003813"
     CDS             complement(1131..1988)
                     /gene="sitC"
                     /locus_tag="ACHU2L_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR1358459.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1985..2812)
                     /gene="sitB"
                     /locus_tag="ACHU2L_003814"
     CDS             complement(1985..2812)
                     /gene="sitB"
                     /locus_tag="ACHU2L_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR1358460.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2812..3726)
                     /gene="sitA"
                     /locus_tag="ACHU2L_003815"
     CDS             complement(2812..3726)
                     /gene="sitA"
                     /locus_tag="ACHU2L_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR1358461.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4158..>4793)
                     /locus_tag="ACHU2L_003816"
     CDS             complement(4158..>4793)
                     /locus_tag="ACHU2L_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1358462.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1269 a         1173 c         1133 g         1218 t
ORIGIN      
        1 ccgtcagcgg gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa
       61 ttttatcgtg gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc
      121 tggtaaactt tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat
      181 tttttgcatg atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct
      241 gtaggctggc ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg
      301 tgtatgggcg tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa
      361 cacgaccgcg aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag
      421 ccagacacca gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg
      481 aaagcgttgt gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag
      541 aatgatacct actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc
      601 ataatgcagc catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa
      661 cagcaaaaaa tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac
      721 cgtctggatt atgtcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag
      781 atgtacgtct ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa
      841 aacaatcccc ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc
      901 caccgcgcag aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca
      961 ggtaagcacg atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc
     1021 tttcaggacc agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag
     1081 cagtgcgtga ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga
     1141 tcctccagcg ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat
     1201 acaaatgcta aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg
     1261 tccagaaagt aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca
     1321 attatgagca ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc
     1381 accagacaga tcaccagaaa ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac
     1441 agcagggtga agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga
     1501 ttctcgtcga aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag
     1561 agaatgccaa tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc
     1621 aggacgatgg tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg
     1681 ccaaaaaagg acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg
     1741 ttaagaaaca gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac
     1801 ggaagcccca gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg
     1861 ccaatcagcg accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg
     1921 accattgccg agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc
     1981 acgttcatct tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat
     2041 cgtcagtaat aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa
     2101 aagccagctc caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg
     2161 tgcctttgac catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg
     2221 agacaagcat cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt
     2281 cggttttcac atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg
     2341 caattgctct cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac
     2401 gatggcgaaa atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc
     2461 tctttttggc tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt
     2521 ccaccagtac aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt
     2581 ttttctgtag cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac
     2641 gcacaaatcc cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca
     2701 gagcggcaat agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt
     2761 tacgccaggt gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc
     2821 ccttttcccg gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat
     2881 atacgtaggt accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta
     2941 gtgtgcgccg gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct
     3001 aaagactgcc gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg
     3061 tgttccttgt tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg
     3121 tgcgagataa gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc
     3181 cgtaatctgc ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc
     3241 attacgttgg taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt
     3301 atcgacgtaa atcagagcat tatctggcga catccatgca tggggattag gtttgccctc
     3361 atagggtcct tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac
     3421 cccattgaga tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag
     3481 aatcagttgt gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat
     3541 ttctgcacca ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc
     3601 catatccgcg atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg
     3661 aaatgcgatc gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga
     3721 gtgcataatt tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt
     3781 tatagcataa cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca
     3841 gggattaggt tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta
     3901 ggctttgaaa tgactttatg aggaaaattg ttgatggcaa aaatggggca aaaaagagtg
     3961 ggttatcgta gcttattgtt gtcgctgata atgtttaaca tattgaaaaa taaataaaat
     4021 acttatgagt cagatagttg tgatttttgc ccttacttgt tcaggttgta ttgttctttc
     4081 tttgtaattt gttgattttc ttgcattatt tcagttctct ggtactaaat ggggcaaatt
     4141 gggggcaaac tttgcaacta cgataaccgc gcattcaaca tggctatctg ttcgtcgttc
     4201 atgtcatcaa tccacatacc gtaaatttca tacaccattt gcgcagtttc atgccccatc
     4261 tggctggcta taaatgccgg gttcgctcct gccgtcaaca gccagcaggc aaaagtatgt
     4321 cgcgtatggt acggattacg gcggcgaata ccagcacgtt ttactgctgc attccatctt
     4381 gtacccaaac tgcttaccga gtaataaggt ttctgttttt cgttacacac cctgggcatg
     4441 aaaacaaaat gcagtttttg tttttcagtt ctgccgtact cccgatgata aaaagtgatt
     4501 tcgcttttgc gatgatgccc ggtcagtttg tattgctcct tcagtgcttc aagagccggc
     4561 tgcagtaatg ctaccgtccg gatcccggca tttgtttttg ggggaccgaa catatcaagt
     4621 atcgtcaggt ttcttctgac attcacaatt cccttctcga aatccacatc ctcccacgcc
     4681 agagctgcca gttccccgtg acgaagcccg gagtaaacgg caaatttcca caagttttgg
     4741 ctctgtcctt tttcactttc cattaatgca ttgaattctg ttttagataa cgg
//
LOCUS       DAZPEU010000245         4780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPEU010000245 DAZPEU010000000
VERSION     DAZPEU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.247"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="ACHU2L_003817"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="ACHU2L_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR1358463.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="ACHU2L_003818"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="ACHU2L_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR1358464.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACHU2L_003819"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACHU2L_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR1358465.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHU2L_003820"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHU2L_003820"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR1358466.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHU2L_003821"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHU2L_003821"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR1358467.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..>4780
                     /locus_tag="ACHU2L_003822"
     CDS             4449..>4780
                     /locus_tag="ACHU2L_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR1358468.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRA"
BASE COUNT         1299 a         1228 c         1089 g         1164 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc
//
LOCUS       DAZPEU010000246         4672 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPEU010000246 DAZPEU010000000
VERSION     DAZPEU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.248"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..77
                     /locus_tag="ACHU2L_003823"
     CDS             <1..77
                     /locus_tag="ACHU2L_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358469.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(91..282)
                     /locus_tag="ACHU2L_003824"
                     /pseudo
     CDS             complement(91..282)
                     /locus_tag="ACHU2L_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            280..604
                     /locus_tag="ACHU2L_003825"
                     /pseudo
     CDS             280..604
                     /locus_tag="ACHU2L_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(634..3546)
                     /locus_tag="ACHU2L_003826"
     CDS             complement(634..3546)
                     /locus_tag="ACHU2L_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR1358470.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            4029..>4672
                     /locus_tag="ACHU2L_003827"
     CDS             4029..>4672
                     /locus_tag="ACHU2L_003827"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1358471.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVA"
BASE COUNT         1208 a         1165 c         1134 g         1165 t
ORIGIN      
        1 gggccttccc gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga
       61 gccagtcagt tgcttaactg actggcatta aggccgccgg ggacgttttt ttaccaccgg
      121 gagtttgggc ataacggtgc tttcagtaag tacgacaggt actgtggagg cacttccgta
      181 aagaagggca tcaagctcct gctccgacat acctgcaggc aaaggccatg aaagccctga
      241 tttccggaag cgagcgaaca gtccacaaac tgttgaacgt ggtgtcagcg gcggacaggg
      301 ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg ttctttaccc ggtcaaaaca
      361 gcgttgcggt gcaccacgcc tgacggatga actgcgtgct cagggttacc actttaacgt
      421 aaaaaccgtg gcggcaagcc tgcgccgtca ggggctgagg gcaaaggcct cccggaagtt
      481 cagcccggtc agttaccgcg cacatggcct gcccgtgtca gaaaatctgc tggagcagga
      541 tttttacgcc agtggtccga accagaagtg ggcgggagac atcacgtact tacgtacaga
      601 tgaagttcgg ttacatcctg tcagcacgga acctcacgct ttttgagcgg cacgacggat
      661 cccccccacc cagtatccga cagacttttc actgcgttcg taaaaaccct tcttccctcc
      721 cagcatatcc actaattccg gtgacatatc ctgcagataa gtctgcaaat catgccccag
      781 atcttccggt gactgcaact cataaaccgc gcgccacaca tcacccttcc ttattttaag
      841 ccccgggtta agaatatact gtccgtggtt cagcctcaga aaaagctttt ttccataagg
      901 atttttgctg ttaacttcat gttgagagag ctggctgctg atataactcc ttcgtttgcg
      961 gcgttccggc agcaacgctt caggaagatg aaccacacac tctgccagtc gtgccgcact
     1021 gaaggcttcc gaaaaaaaca gagcacttcc cagatgcgtg tggaacagcg cctgcatcag
     1081 gatcaccaga aacattgcca tctgccggtt gtccagtttg atgaggcgtc cttccacctg
     1141 cagtgacaga ctttcaggtt tcagcagggt atacaactgc gcacttttct gttgcgcata
     1201 acggggcgta ctgaccgccg ctgacagaac ttgatgataa gccgccagcc cgtcattacc
     1261 ggtcagcaag ggatctgcac cacgctccag cagtaactga accagtgcaa tattacctgc
     1321 ccgggctgct gacatcagcg gagtaagatt aaacggcgaa cggtgatcaa caccataacg
     1381 ttcaatatcc cgcaacacgg caaccgggtt attcattgag tatggccgga aatacttcag
     1441 ctccagtaac tgcagagtct tcgttctggg ctgccttgtg ggcttaaaat cttcccggta
     1501 cagtgcatta tacagcggga acagggaata aatcagactg tactccagcg cctgcagttg
     1561 tatggtttta tcggctgtac ctgcgaggat atgctgtttc cactgtcgtg catcctctgc
     1621 ggtgataatc tgccatggca tctcagatgt ctgcaggatc cggctacgga tggtgtccgc
     1681 ctgctcctgc ttcccctgtt tttcaagcag atgtgcggtt ttctgccact catcacggct
     1741 ggaaatctct tcctgcagat tcagaggctc ttcctgatgc gtgagcgcaa gcagactcca
     1801 cagaggatgc tccacctgct gttcaacaag atacacattg tgtttagcac gggtgagcgc
     1861 aacatacagc gcgttggtaa aaaatttata gatttccgct gaacgatcct gcctgtcccg
     1921 cggacgggaa taacgggcct cgccctcaag atcagccggt gtaagaccct cgcaaatatc
     1981 atcaaatgcc tgccgggcag cgcttactat attgtaaagg atcaccgttt cgtattccag
     2041 ccccttaacc tcctgaaccg aaaagaccag cggcgtactg aaccagcatc gcgcctgtgc
     2101 tttctgttcc ggatgcatca caatgaccgc tacacgattc gaaagtgagg ttttggcatt
     2161 cagttcctgc tttgtctctt ccctatcgtc cagcaaccgg atggtccctt ctgcctgacc
     2221 gcagcttctg acaaaatggt gactttcccg atcaatagca ctaaagcgga cctgtttgag
     2281 tctcagcaac cggttagcca gtgcagtgac gtggtgcccg ttacggtaat tcgcctgcag
     2341 gatattaacg gttgtgtcgt ttccctgttg ctgccggaaa aagagacttt tgagcgaact
     2401 ccatgagaaa aagctcggat gaactatctg attggcatca ccacagagta aaaactgacc
     2461 gggatgacgt agcgttttca gtaccagttg cagttgaaca ggcgtcatat cctgaacttc
     2521 atcaacaaaa atgacatcat aacggggagt tgccaacgaa agatactgat gactgagcag
     2581 attactgtca tactgatgcg actgtttcag ccatgcgata taacgttcaa acaggacata
     2641 gaccgtcggg cggtcctcca tgccatagag cgactgccgt atccccagtg acagatacgc
     2701 ctcccgggac agcggcccat tcccggaggc aaccgcaccg atcacgcccc tgaactcctc
     2761 atacagggta tgggcagccc ccagtgcggc gatagccctg ttgcggggca gccagtcgct
     2821 gaacgcactg aaagtcacct ccctgccttc cggaatgcgc agagtttcca gaaattccgt
     2881 cagcgaaaga aagtcaatat tctgtacctc actcccttcc cctgaagcgt catagagagt
     2941 acgcgctttt tccaccagaa atgaggaaag gctgagatac agtatatcac cagcagcctg
     3001 cttcattttt tccagtacca gcgccgtctt accacttccc gccgagccaa caatcaccag
     3061 cggtagcggg tgttcgtaca gtgcctgctg atcatcatca aaggacagca ttttatccag
     3121 gcggtggaaa cgcccatgag acggattgat atacgtcagc gcttcagtcg cgatatcaac
     3181 aggatccggc actggttgtt gttgtaagcg cccttcatca atcaccacgt ttctgcgaag
     3241 aaaacgtgaa gtgttatagg catgattgcg aatatattcc agaacaaggc agatagtttc
     3301 accgtggtag cgatacagcg aaaacagtaa tcggtcccgg atactgagcc gggcgcggta
     3361 aagattggtg tcaattttac gaacatcagc ctgaacaaag ttatcggtgg caatagcctg
     3421 tgcaaatttt ttataacccg ggatcgtgtc aggattcaga gtatgataaa gacagatttt
     3481 catggctgcc ggtttccgtc gtgcaaaaaa catgctgatt ttacgaagga atgccttact
     3541 gaacaacctt gcccggaata ccccccgaaa tcagatggaa caatcagaaa aatgctctgc
     3601 agttatggaa taaagaaggt ggggtggtac gccgggaggg cgatccgctg gaaatggcgc
     3661 agtagctgca ggcctgtcat gatgcaggaa tagaagttcg tgtacaggtt aatgaaagcg
     3721 tcaccccgta aataaactct gcgctgaaat tccttttttt cattggagta cctgtgttgt
     3781 tctgaggtga acatatcacc tctgttcagg tagccaaatt cagtgtgcca cctcaaggtt
     3841 cttagtgagt cgtttttaaa agatatgata atatatcatt gtttatgaat tgttgatgtt
     3901 taatttagta aattatatca aatatgccat gggtttgcta tgttattcat ccagtatctg
     3961 gaaataaaat gcaccagctt tattgggttt tccttttgtc tctgcttgct aggtgccaga
     4021 aactttaaat gttaaacgaa gggggcattt tgttcaaagc caatcatgtt ccggtattaa
     4081 tgtatcatca tgtcagtcat tgccccggac tggttacact ttctcccgtg acattccgga
     4141 agcaaataaa atggttggct gaaaacaact ggaaaacact gtcatcagat gaactggagt
     4201 ttttttatcg gggcggaaaa ctcccccgga aaagcgttat gttgacattt gatgatggat
     4261 accttgacaa ctggtttcag gtatatcctt tattaaaaga gtttaatctc aaagcacata
     4321 tttttttaat taccggtttt attgggaatg gtccggttcg tcactctcct ggaaaggaat
     4381 attctcatcg cgattgcgaa catcaaatag cgacagggaa tgctgataat gttatgcttc
     4441 gctggtctga agtaaacgaa atgttgcaat ctggtctggt cgaatttcat gttcatacgc
     4501 atactcatac acgctgggat aaaaagttca gttcacgaga agaacagtgt aaacatcttc
     4561 ggcaggactt gttgagcggg agggaatatt tgaaagaaat gacaggaaaa tgcagtaaac
     4621 atttgtgttg gccggaaggt tattataaca aggattatat tcaggtcgct ga
//
LOCUS       DAZPEU010000247         4602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPEU010000247 DAZPEU010000000
VERSION     DAZPEU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.249"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHU2L_003828"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHU2L_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR1358472.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHU2L_003829"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHU2L_003829"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR1358473.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHU2L_003830"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHU2L_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR1358474.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHU2L_003831"
     CDS             complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHU2L_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR1358475.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAZPEU010000248         4520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPEU010000248 DAZPEU010000000
VERSION     DAZPEU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.250"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            235..1092
                     /gene="focA"
                     /locus_tag="ACHU2L_003832"
     CDS             235..1092
                     /gene="focA"
                     /locus_tag="ACHU2L_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR1358476.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1147..3429
                     /gene="pflB"
                     /locus_tag="ACHU2L_003833"
     CDS             1147..3429
                     /gene="pflB"
                     /locus_tag="ACHU2L_003833"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1358477.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3621..4361
                     /gene="pflA"
                     /locus_tag="ACHU2L_003834"
     CDS             3621..4361
                     /gene="pflA"
                     /locus_tag="ACHU2L_003834"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR1358478.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4443..>4520)
                     /locus_tag="ACHU2L_003835"
     CDS             complement(4443..>4520)
                     /locus_tag="ACHU2L_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358479.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1151 a         1113 c         1093 g         1163 t
ORIGIN      
        1 gcctgatgct cccttttaat taactgtttt agcggaggat gcggaaaaaa ttcaactcat
       61 ttgttaattt ttaaaattta tttttatttg gataatcaaa tagttactcc gtatttgcat
      121 aaaaaccatg cgagttacgg gcctataagc caggcgagat atgatctata tcaatttctc
      181 atctataatg ctttgttagt atctcgtcgc cgacttaata aagagagagt tagtgtgaaa
      241 gctgacaacc cttttgatct tttacttcct gctgcaatgg ccaaagtggc cgaagaggcg
      301 ggtgtctata aagcaacgaa acatccgctt aagactttct atctggcgat taccgccggt
      361 gttttcatct caatcgcatt cgtcttctat atcacagcaa ccactggcac aggcacaatg
      421 cccttcggca tggcaaaact ggttggcggc atttgcttct ctctggggct gattctttgt
      481 gttgtctgcg gagccgatct ctttacttcc acagtgttga ttgttgttgc taaggcgagt
      541 gggcgcatca cctggtgtca gttggctaaa aactggctaa atgtctattt tggcaacctg
      601 gtcggcgcac tgctgtttgt acttttaatg tggctttccg gcgagtatat gaccgcaaat
      661 ggtcaatggg gactaaacgt cctacaaacc gccgaccaca aagtgcacca tacttttatt
      721 gaggccgtct gtcttggtat cctggcaaac ctgatggtat gtctggcagt atggatgagt
      781 tattctggcc gcagcctgat ggacaaagcg ttcattatgg tgctgccggt cgcgatgttt
      841 gttgccagcg gttttgagca cagtatcgca aacatgttta tgatcccgat gggtattgta
      901 atccgcgact tcgcatctcc ggaattctgg accgctgtcg gttctgcacc ggaaaatttt
      961 tctcacctga ccgtgatgaa cttcatcact gataacctga tcccggttac gatcggtaat
     1021 attatcggcg gtggtttgtt ggttgggttg acatactggg tcatttacct gcgtgaaaac
     1081 gatcaccatt aatggttgtc gaagtacgca gtaaataaaa aatccactta agaaggtagg
     1141 tgttacatgt ccgagcttaa tgaaaagtta gccacagcct gggaaggttt taccaaaggt
     1201 gactggcaga atgaagtaaa cgtccgtgac ttcattcaga aaaactacac tccgtacgag
     1261 ggtgacgagt ccttcctggc tggcgctact gaagcgacca ccaccctgtg ggacaaagta
     1321 atggaaggtg ttaaactgga aaaccgcact cacgcgccag ttgattttga caccgctgtt
     1381 gcttccacca tcacctctca cgacgctggc tacatcaata aagcgttgga aaaagttgtt
     1441 ggtctgcaga ctgaagctcc gctgaaacgt gctcttatcc cgttcggtgg tatcaaaatg
     1501 atcgaaggtt cctgcaaagc gtacaaccgc gaactggacc cgatgatcaa aaaaatcttc
     1561 actgaatacc gtaaaactca caaccagggc gtgttcgacg tttacactcc ggacatcctg
     1621 cgttgccgta aatccggtgt tctgaccggt ctgccagatg cttatggccg tggccgtatc
     1681 atcggtgact accgtcgcgt tgcgctgtac ggtatcgact acctgatgaa agacaaatac
     1741 gctcagttca cctctctaca ggctgatctg gaaaacggcg taaacctgga acagactatc
     1801 cgtctgcgcg aagaaatcgc tgaacagcac cgcgctctgg gtcagatgaa agaaatggct
     1861 gcgaaatacg gctacgacat ctctggtccg gctaccaacg ctcaggaagc tatccagtgg
     1921 acttacttcg gctacctggc tgctgttaag tctcagaacg gtgctgcaat gtccttcggt
     1981 cgtacctcca ccttcctgga tgtgtacatc gaacgtgacc tgaaagctgg caagatcacc
     2041 gaacaagaag cgcaggaaat ggttgaccac ctggtcatga aactgcgtat ggttcgcttc
     2101 ctgcgtactc cggaatacga tgaactgttc tctggcgacc caatctgggc aaccgaatct
     2161 atcggtggta tgggcctcga tggtcgtacc ctggttacca aaaacagctt ccgtttcctg
     2221 aacaccctgt acactatggg gccgtctccg gaaccgaaca tgaccattct gtggtctgaa
     2281 aaactgccgc tgaacttcaa gaaattcgcc gctaaagtgt ccatcgacac ctcttctctg
     2341 cagtatgaga acgatgacct gatgcgtccg gacttcaaca acgatgacta cgctatcgct
     2401 tgctgcgtaa gcccgatgat cgttggtaaa caaatgcagt tcttcggtgc gcgtgcaaac
     2461 ctggcgaaaa ccatgctgta cgcaatcaac ggcggcgttg acgaaaaact gaaaatgcag
     2521 gttggtccga agtctgaacc gatcaaaggc gatctcctga gctacgatga agtgatggag
     2581 cgcatggatc acttcatgga ctggctggct aaacagtaca tcactgcact gaacatcatc
     2641 cactacatgc acgacaagta cagctacgaa gcctctctga tggcgctgca cgaccgtgac
     2701 gttatccgca ccatggcgtg tggtatcgct ggtctgtccg ttgctgctga ctccctgtct
     2761 gcaatcaaat atgcgaaagt taaaccgatt cgtgacgaag acggtctggc tatcgacttc
     2821 gaaatcgaag gcgaataccc gcagtttggt aacaatgatc cgcgtgtaga tgacctggct
     2881 gttgacctgg tagaacgttt catgaagaaa attcagaaac tgcacaccta ccgtgacgct
     2941 atcccgactc agtctgttct gaccatcact tctaacgttg tgtatggtaa gaaaactggt
     3001 aacaccccag acggtcgtcg tgctggcgcg ccgttcggac cgggtgctaa cccgatgcac
     3061 ggtcgtgacc agaaaggtgc tgtagcgtct ctgacttccg ttgctaaact gccgtttgct
     3121 tacgctaaag atggtatctc ctacaccttc tctatcgttc cgaacgcact gggtaaagac
     3181 gacgaagttc gtaagaccaa cctggctggt ctgatggatg gttacttcca ccacgaagca
     3241 tccatcgaag gtggtcagca cctgaacgtt aacgtgatga accgtgaaat gctgctcgac
     3301 gcgatggaaa acccggaaaa atatccgcag ctgaccatcc gtgtatctgg ctacgcagta
     3361 cgtttcaact cgctgactaa agaacagcag caggacgtta ttactcgtac cttcactcaa
     3421 tctatgtaat tagatttgac tgaaatcgta cagtaaaaag cgtacaataa aggctccacg
     3481 aaagtggggc cttttttagc acgagagcct tttttgtcag ctatctatac tttaaggtga
     3541 ctgccaaaac agactcgacg tagccttcga gctgcgcacc aacacggcct cagatgggcc
     3601 acatctggag aaacaccgca atgtcagtta ttggtcgcat tcactccttt gaatcctgtg
     3661 gaaccgtaga cggcccaggt attcgcttta tcaccttttt ccagggctgc ctgatgcgct
     3721 gcctgtattg tcataaccgc gacacctggg acacgcatgg cggtaaagaa gttaccgttg
     3781 aagatttgat gaaggaagtg gtgacctatc gccactttat gaacgcttcc ggcggcggcg
     3841 ttaccgcatc cggcggtgaa gcaatcctgc aagctgagtt tgttcgtgac tggttccgcg
     3901 cctgtaaaaa agaaggcatt catacctgtc tggataccaa cggttttgtt cgtcgttacg
     3961 atccggtgat tgatgaactg ctggaagtaa ccgacctggt aatgctcgat ctcaaacaga
     4021 tgaacgacga gatccaccaa aatctggttg gagtttccaa ccaccgcacg ctggagttcg
     4081 ctaaatatct ggcgaacaaa aatgtgaagg tgtggatccg ctacgttgtt gtcccaggct
     4141 ggtctgacga tgacgattcg gcacatcgcc tcggtgaatt tacccgtgat atgggtaacg
     4201 ttgagaaaat cgaacttctc ccctaccacg aactgggcaa acacaaatgg gtggcgatgg
     4261 gtgaagaata caaactcgat ggtgttaaac caccgaagaa agagaccatg gaacgcgtga
     4321 aaggcattct tgagcagtac ggtcataagg taatgttcta atttcctcgt aaagcggcaa
     4381 ctttattgag ttgccgcttt tttattccgc gcttcattct gctacagcgt ttttaacaca
     4441 tcttatagtg catctaccat atcccgtacc tgagataata acgactgata atagctcgcg
     4501 tgaagttctt ccccatcgaa
//
LOCUS       DAZPEU010000249         4404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPEU010000249 DAZPEU010000000
VERSION     DAZPEU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.251"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..615
                     /gene="ospD1"
                     /locus_tag="ACHU2L_003836"
     CDS             <1..615
                     /gene="ospD1"
                     /locus_tag="ACHU2L_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR1358480.1"
                     /translation="SNTANENKGMKNNIINVTNTAISHAINEEKSGGGYSGVSFRKLA
                     KIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGESLLAAEIPKEEKLEVLAARNN
                     AGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIKLLDVRDNEGLPGLFLAAGKGN
                     IEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIISDKIQELF"
     gene            939..1049
                     /locus_tag="ACHU2L_003837"
                     /pseudo
     CDS             939..1049
                     /locus_tag="ACHU2L_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1127..1441
                     /locus_tag="ACHU2L_003838"
     CDS             1127..1441
                     /locus_tag="ACHU2L_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358481.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            1723..2289
                     /gene="ipgB2"
                     /locus_tag="ACHU2L_003839"
     CDS             1723..2289
                     /gene="ipgB2"
                     /locus_tag="ACHU2L_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR1358482.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2551..2673)
                     /locus_tag="ACHU2L_003840"
                     /pseudo
     CDS             complement(2551..2673)
                     /locus_tag="ACHU2L_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2816..3370
                     /locus_tag="ACHU2L_003841"
                     /pseudo
     CDS             2816..3370
                     /locus_tag="ACHU2L_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            3709..4070
                     /locus_tag="ACHU2L_003842"
                     /pseudo
     CDS             3709..4070
                     /locus_tag="ACHU2L_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4157..>4404
                     /locus_tag="ACHU2L_003843"
     CDS             4157..>4404
                     /locus_tag="ACHU2L_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1358483.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT         1331 a          925 c          982 g         1166 t
ORIGIN      
        1 agcaatactg ctaatgaaaa taaaggaatg aaaaataata tcattaacgt gacaaatacc
       61 gctatatccc acgccatcaa tgaagaaaaa tcaggggggg gatatagtgg tgtttctttc
      121 agaaaattgg ccaaaataca gaacatatcc attccgacaa agaataataa ggagtataac
      181 cgccataatt tgttttcatt gatttggcat ggaaatgccg atgcagcgcg taaatacggt
      241 gaatcgctgt tggcagccga aatacccaaa gaggaaaaac tagaagttct tgcagcacga
      301 aataatgctg gggaatctgc tttgttcata gctcttcaag aaggtcattc cgctgcgatt
      361 caagcttatg gagattttat taaaactttt gatttatcac caaaagaaac gattaaacta
      421 ttggatgtaa gagataatga ggggttacca ggattatttc tggccgcagg gaaagggaat
      481 atcgaggcta tgatggcata tataaatata tgccatcata gtgggataaa acttacagaa
      541 atagcagaca gacttaacaa taatgaacaa gacatgttta atattatttc tgacaaaata
      601 caagagttgt tttaagtgtg ctaaatagct gcaaagaatt gcacttagaa ggatctctcc
      661 ccgaattgtg tgatctgctt aatttctaaa aagaaacaaa cagttaacta gactgagcga
      721 tactacccat agaaccagtt tcccatgacg aacgacactg gacgcaaaaa caatcataaa
      781 aaacacaata aatgtaatga acacatccca ccctattaag taaagtgcac tgtgatagag
      841 gaagggaaat acaggaaggt gtggagcctt gcagatttct ggcatcatag tgcccaatac
      901 ggaatgcata aagccctctc agactggaga ctccacaaat gaaatataca gtgtttaatt
      961 atatcaagtg cgattacaat cgctgacgac ggcacagttg gtgtggaggt ctcagtcctg
     1021 aacaatttga aaaccagaac ctcacttaag cctgtgttca tattacgtgg gtaggatcat
     1081 acttttatag aagctgtcag cggctacgtc ccagccaaat cacttcatgc tccagcgcca
     1141 gagaggcaag gttgggtttg ctcagctgcc ggttgatttt gttgcgattg aaccggacag
     1201 cgtccagggc gtcggcaaaa gagcgaatct caccaatcgt tgtttcatta tccgcattaa
     1261 cgatagcttc aagaaacgcc aaggttttat cgaatttatc agcaacagtg gctccgggca
     1321 taccgtgacc gtttatacaa aacggcgttt tcagcgtgga gttttcatga atagcctcaa
     1381 cacgaatgtc gtgaagccag tgatgtatcg gctacgatcc tcagctgatg ccagaccata
     1441 ggactgacac tacgaacagc aattttaatg acgtaaatct tcataactac aactaacagg
     1501 gcatcattat gaatgatgcc ggttatttta ccgctgttgg tcccttcatt tagggactgc
     1561 cccaagtttt ttcatactct ctttctcaga gagtttagtc tctgggattc cagacggtcc
     1621 atcattgagc caagggagta aagaactcaa agcatttaca gaattgtttg aatcagctaa
     1681 aataatgaca atgttactat tttacatgac aaggtgatta taatgcttgg aacatctttt
     1741 aataattttg gaatcagttt atctcacaaa agatattttt caggaaaggt ggatgagatt
     1801 atccgctgta caatggggaa aaggatagta aaaatatcat caaccaaaat aaatactagt
     1861 attttgagct ctgtttcaga acaaatcggt gaaaatatta cagattggaa aaatgatgaa
     1921 aaaaaagtct acgtatcccg ggtggtaaac caatgtattg ataagttttg tgcagaacac
     1981 agccgaaaaa tcggcgataa tctccgtaaa cagatattta aacaggttga gaaagattat
     2041 cgcatttctc tagacatcaa tgctgcacaa agttccataa atcatcttgt atcaggaagc
     2101 agttatttca aaaagaaaat ggacgaactt tgtgaaggga tgaatagaag cgttaaaaat
     2161 gatacaacaa gcaatgttgc aaatcttatt tccgatcagt tttttgagaa aaatgtgcaa
     2221 tatattgatc taaagaaatt acgtgggaat atgagtgact atattacaaa tttagaatcg
     2281 cctttctgat gacagaacat cccgagtaaa gaaactaaga aaagtaaata cccaggaatt
     2341 gcctgagcgg tgctatattt ctcctcgcaa gtaagtccta atatattgaa ttgttgtata
     2401 gccagttttt gtattttcct agctcatgga tgtgggatcc ctctcccctt tctctgctca
     2461 aaggtaagcg gctcgtcaga atcatattga tatttactga gaagtcagat caactttcca
     2521 aggcaacaga tcgcgtaccc ggtttgccgg tcagtcctgg atatgttcaa taacgtaacg
     2581 cagccatttt tctggctcca cattgttcag acggcatgtg ccgatcagcg agaacaacac
     2641 cgctgcatgt tcgtcaccgc tgtcggaacc tgcgagtcga gtcagccgga gagagtttca
     2701 ctctccggct gcttacggaa ccgtacgtga cactctcgcg tcatacggct cttgatcact
     2761 cattcgatgc acgtagtaaa taccagtgag caaattgatc ttacccagca atacggtcag
     2821 ttaccgcgca catggcctgc ctgtgtcaga aaatctgctg gagcaggatt tttacgccag
     2881 tggtccgaac cagaagtggg cgggagacat cacgtactta cgtacagatg aaggctggct
     2941 gtatctggct gtggtcattg acctgtggtc acgtgccgtt attggctggt ccatgtcacc
     3001 acgcctgacg gcgcaactgg cctgcgatgc cctgcagatg gcgctgtggc ggcgtaagag
     3061 accccggaac gttatcgttc actcggaccg aggaagtcag tactgttcag cagattatca
     3121 ggcgctgctg aagtggcata atctgcgtgg cagtatgagc gcaaagggct gctgctacga
     3181 taatgcctgc gtggaaagct tctttcattc gctgaaagtg gaatgtctcc acggagaaca
     3241 ctttatcagc cgggaaataa tgagggcaac agtgtttaat tatatcgaat gtgattacaa
     3301 tcgctggcga cggcacagtt ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa
     3361 cctcgcttag gcctgtgtcc atattacgtg ggtaggatca cttacgccct gtggttgaaa
     3421 cgtgagcata ttgttttcag atgctgttgt cttgcttcag agtgatctgt cctccggatt
     3481 tacgcaaagt tatccacagg cactgtgcat ttaaaaaact tatcttttct tcttgatttt
     3541 aaaaactcag atcgtcatat cattatgata aacataataa aaaactctaa acatgagaag
     3601 gtttttaacg taaggtgaga ggatttttaa gtgtatccat agattaaaaa tgacaatgcc
     3661 tccaattagt tgaattaggg gttataatac ccgcttttag gttctttcat ggtcacagtt
     3721 aactttcatt gcccccgttg ccagtccgtt caggtttacc gtcacgggca gaacccaaaa
     3781 ggacatgatc gattccgttg ccgtgactgt caccgggtgt ttcagctcac ttactgttac
     3841 gaggcccgta aaccgggagt taaaaaccaa atcactgaaa tggctttcaa cggtgccgga
     3901 gttcgcgata ctggccgtac gctgaaaatc ggcatcaata ccgtcatacg gacattaaaa
     3961 agctcccgcc ccggcggata acatctgcgc cagtcgctca cgctgatgtt gcccttatct
     4021 gtgaacttga tgggcagtgg agcttcgttg gcagcaaggc gcaacaacac tgtcacgaac
     4081 ggtgcaatag tgatccacac ccaacgcctg aaatcagatc cagggggtaa tctgctctcc
     4141 tgattcagga gagcttatgg tcacttttga gacagttatg gaaattaaaa tcctgcacaa
     4201 gcagggaatg agtagccggg cgattgccag agaactgggg ctctcccgca ataccgttaa
     4261 acgttatttg caggcaaaat ctgagccgcc aaaatatacg ccgcgacctg ctgttgcttc
     4321 actcctggat gaataccggg attatattcg tcaacgcatc gccgatgctc atccttacaa
     4381 aatcccggca acggtaatcg ctcg
//
LOCUS       DAZPEU010000250         4403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPEU010000250 DAZPEU010000000
VERSION     DAZPEU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.252"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(34..360)
                     /locus_tag="ACHU2L_003844"
                     /pseudo
     CDS             complement(34..360)
                     /locus_tag="ACHU2L_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(370..918)
                     /locus_tag="ACHU2L_003845"
     CDS             complement(370..918)
                     /locus_tag="ACHU2L_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR1358484.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(918..1625)
                     /locus_tag="ACHU2L_003846"
     CDS             complement(918..1625)
                     /locus_tag="ACHU2L_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1358485.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1640..2317)
                     /gene="ydjY"
                     /locus_tag="ACHU2L_003847"
     CDS             complement(1640..2317)
                     /gene="ydjY"
                     /locus_tag="ACHU2L_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR1358486.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2322..3032)
                     /locus_tag="ACHU2L_003848"
     CDS             complement(2322..3032)
                     /locus_tag="ACHU2L_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1358487.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3199..4005)
                     /gene="xthA"
                     /locus_tag="ACHU2L_003849"
     CDS             complement(3199..4005)
                     /gene="xthA"
                     /locus_tag="ACHU2L_003849"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR1358488.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         1122 a         1122 c         1092 g         1067 t
ORIGIN      
        1 aatgccagtc agttgcttaa ctgactggca ttactctttt aaggtgcgga agttttcgcc
       61 gttcacccag agcagatcca ccgagccgcc cgttttacga ccggcagcag cttcggtctg
      121 aatgcgcttc accgcgtctg cggcatccgc gagacgaaca atcttcaggt ttatagcgta
      181 atgggttttc atctcgccgc taacccagtc gagatagcgg ttaattgcgg tatcgccgcc
      241 ccaggcgtta aaccagacgg tttgcccttt ggcctcattt ttaatttcct gccagtcact
      301 ggcatgtgtt gccaaagaaa acagcgataa caatcccagc aaccacccac aatggcgcat
      361 cttccctcct taagaacgtt tttccggaat acggcacagc ccctgagcgg gaatgtccat
      421 ggcagaatta aaacgagccg acaggttttg caggccgatc aatgccgtca gttcggtgag
      481 cgcctgagcg tcaaaatgcg cagccagtcg ggtacgcagg gcatcatcga ctgttggcgg
      541 tgtgacgctt gcggcttcgg cgtactccag cgccagccgt tcttcatcgc taaagagcgg
      601 gctttgccgc cagtcagcca ctgccagcag tttatcggtg ctgccggtac gctcggcgac
      661 tttcatactg gtgatatcca cgcaaaactc acacaggcac atttgcgcaa tgcgcgcgct
      721 gacaagcgat cgcactaccg gatcgagcgg tgagcgtttg cgctccagcc acccaacaaa
      781 cattgacacc aaataaaaga taaacgggat ccgcccccac cagcgaatcg ggcttagcac
      841 ctcgccgtaa tggcgacgat gcagccacgc ctgcggacgt aaaatgaaag gaattttgct
      901 aagcggcggt aaacccatca gtcattcctc ttctggcgct caagccatat tttcttcgcc
      961 ataaaaatca ccacggtcag ggcaaacaga ataaacagtt cggtgacaaa ccagaaagta
     1021 ccgcctgtta acatgctgcc tgcccaggaa taaacaatag tcgccggtaa ttgaccaagc
     1081 ccggtggcga taaaaaacga gcgaaaacgt attgaagtca gaccggcagc atagctgatt
     1141 ggatcgaaag ggacaaaagg caataaccga cagaccagga tggtgtgttt gccgtagcga
     1201 gtgaaaaagc cgtccatact gtcaagcacg gttttgccgg ttaatttttc caccacttcg
     1261 cggcccatca ctctggcgat aaaaaagcac agcgccgcgc cggccatcga actggtccac
     1321 gacagcaagc cgccccagaa cgcgccaaac agcgacgcat tggcaaaggt gatcaaaaac
     1381 gcaggtagcg gtgcagcaat ggcctgtaaa atcatcaaca agaacgagac aactgccgcc
     1441 agtgcgccgt aagactgaat aaagcgttct ataccctgtt ggtccacggc ggcaaacgcc
     1501 gcaacgctgc gattgataaa ctcatggacg ccgggaagca gcgcccatgc gagcagcata
     1561 gcgaacaaca ggatgatgag ggttatacgg tagtaccaga ttttacgcga ttgtatcttc
     1621 atcatccttt atcttggctt tattcggtga ttttaaaggt aaccgttgcc agcgtgttat
     1681 ccgccgggag aactgaggca ttgcctttga atttcacttc gccgtgtttt tccaccgcac
     1741 cataagtgta tgttgcattg ctgacgatgc cgaccgggca gctatccaga cacaccaggc
     1801 aacccgtttt cttctcttct gctgccgtta aattaccgcc aaagcgcatg tccagtttct
     1861 tgccattact gtcaacaatc acttcatcga aggaatacgc ttttgccgcc ccttgccagt
     1921 tgaccgaaat atccagtttg ctgcctgtga catgagtcgt ttctttatta tccatcgtca
     1981 tgttttcgcc cggcgtacca cctgcctctt tcagggcttc ataaaatgct ttcgggggcg
     2041 cataagccat aaacagcgat ttatgtccgt tggagccatc tttaaacaca ataccgtgac
     2101 gagtgtcgtc ggtgaggaaa cgaccattaa tttgcaccag catggttacc gtatggttat
     2161 tggcatcgac gcgcagcggt tggctgttcg aaagtccgtc gtattccact tttgccgccg
     2221 cattctcttt ttgatcgcag ccgctaagcc cggcaacagc cagtaagcag agtgcagcca
     2281 gtcctttttt ccatgacact gaataatgtt gcgacatcgt tctatccttc attttttggg
     2341 tgagtaagtg ggctgcggcg cgaagcagac agatccacat gtttgtgtcg ggcgtagagt
     2401 tttgcgagct ggatgagaat aaacagcgcc aggcccgcca gacagagttg taaaataaag
     2461 cgcagcgtaa tgccttcatt ggcgagatcg cttgccatca cggtataaat aacaataccg
     2521 ggcagggtcg tgagtgccga aataagggta taaggccaga aggcgatggt ggtgaatccg
     2581 taagcgtaat tttgaatatt gtaaggaaac aacgggatta agcgggtcag aataagaaaa
     2641 tcaataccgt tacgcgcaat gcctttttca atggcctgga aggtatggct atggcccacg
     2701 tatttcagca gtaaatcacg tcccagccag cgcgccagca ggaatgagca cgaggaggcc
     2761 agcgttgcgg caattaatga gagttgtgtc cctaagagcg ggccaaaaac tattcctcca
     2821 gcgatcacca gtatgcttcc tggtaacaga aagagggggg cattgatgaa taacagaata
     2881 tagaggctat aaccgaacaa tccgctctgg cgaatgagtg tctgtaagtg gggtaaatcg
     2941 gtgagcagat cgaataaacc gaaagcgtgg atagcgtaaa tgaccagcgc aaaaataaga
     3001 caggcaaaca gaaagttacg ctcagcgttc atcgtggagt ttcctatatc aaacttgata
     3061 atagagaagt ggtcagacaa ctaaaaacgc agtgacggta taacaaagga cggcaggcaa
     3121 caaatcatta ctgacagttt gttgattttt ctcaaggtta attctcctga cccagtttga
     3181 gccaggagag ctgctaaatt agcggcggaa ggtcgcccag acgggggcgt gatcggacgg
     3241 tttttccatg ctgcgaattt catagtcgat gccggtttct acgcaacatt ctgccagcgg
     3301 ttggctggcg agcagcaggt cgatgcgcag accacggtta tcgtcaaaac cttttgagcg
     3361 gtaatcaaac catgagaaac gatctgctgt ttgcggatta gcatggcgga aggtatcgac
     3421 caccccccag ctcatcagcc tgtccatcca ttcgcgctct tccggcagga aagagcattt
     3481 accggtacgc agccagcgct tacggttttc ttcgccaatg ccgatatcca gatctgtagg
     3541 gctgatattc atatcgccca taatcagtac cggattatca cgtttgagtt cggtttccag
     3601 gtagttttgc agattctgat aaaactgcgc ttttgccggg aattttatcg gatggtcgcg
     3661 gctttcaccc tgcgggaagt aaccgttgat cacggtgaca ttacccagca gtgaggggat
     3721 ttccgccata ataatccgcc gctgcgcctc ttcgtcgtca ccgggaaagc cgcgacgcac
     3781 ggcaatcggc gtctctttgg tcagcagcgc cacgccataa tggcctttct gcccgtgata
     3841 aaacacgttg tagccgagct tcgccacctc ttcgagcgga aacatatcgt catgaacttt
     3901 tgtctcctgc aggccaatca catccggttg gtgcttttcg acgatggctt caagctggtg
     3961 aggtctggcg cgcaggccgt tgatattaaa agagacaaat ttcatagtcg ctgccattta
     4021 ttcagataaa gagtgcgtgg atggtagcag aatttcgcgt tgcttaccgc ctgttgttaa
     4081 gtgatttgat cgatggttgt taatgatgtc aacgatggcg caaaaaatgc ccgcttttgg
     4141 tgcgcgctgc gtcagaatgg cgcagtaatt tccagtaaat ttcgtcaatg atcacaattc
     4201 tagaattata ttttacttct gcataacatt gcgtttttta ttctttttat tcacccacat
     4261 cgcaaacgta ttcactttat atgcacttta aatgcatatg gtttggttat aactccttga
     4321 tttccagtta gcctccgccg tttatgcact tttatcactg gctggcacga accctgcaat
     4381 ctacatttac agcgcaaaca tta
//
LOCUS       DAZPEU010000251         4365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPEU010000251 DAZPEU010000000
VERSION     DAZPEU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.253"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(140..886)
                     /gene="cutC"
                     /locus_tag="ACHU2L_003850"
     CDS             complement(140..886)
                     /gene="cutC"
                     /locus_tag="ACHU2L_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR1358489.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(900..1466)
                     /locus_tag="ACHU2L_003851"
     CDS             complement(900..1466)
                     /locus_tag="ACHU2L_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1358490.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1682..3415
                     /gene="argS"
                     /locus_tag="ACHU2L_003852"
     CDS             1682..3415
                     /gene="argS"
                     /locus_tag="ACHU2L_003852"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR1358491.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3457..3501)
                     /locus_tag="ACHU2L_003853"
     CDS             complement(3457..3501)
                     /locus_tag="ACHU2L_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358492.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3592..4080
                     /locus_tag="ACHU2L_003854"
     CDS             3592..4080
                     /locus_tag="ACHU2L_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR1358493.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4198..4266)
                     /locus_tag="ACHU2L_003855"
                     /pseudo
     CDS             complement(4198..4266)
                     /locus_tag="ACHU2L_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         1141 a         1084 c         1067 g         1073 t
ORIGIN      
        1 cgcactgtca cccgctcaac agatcacggt tatcgttcgt tttttatact gctcagggat
       61 aaaaaaaggc ccctgttgaa attgcagggg cctggtacga gcaagcatca tattgggcga
      121 catgatgcaa cggtaaaaat catttggcct gatggcgttc aatgattcct ttcatttcag
      181 caaccgccgc cccgtctacg atataacgcg aatactcgtc cgcgtgttca tctgatgaca
      241 tggacaatcc ttgattacga taacgcatcg gtgaggcttg ccacgctccc gcggagctat
      301 ggacttccag cactccggca tcgaggaagt ggtgcaggtt ttctgcacgg actcctgctc
      361 cagccataat gattggagca tcacgatggg caataagttc cataattttt gataaacctt
      421 gcagtgcgtc tgatttttgc cctgatgtca gtacccgggc aatgcccaat tccgcaagat
      481 tattgagtgt atataaaggg ttagcgcaca tatcgaaggc gcgatgaaaa gtcactgcca
      541 gcggaccggc agcagccatt attttttcca ttcgtggcat atcgacattc ccgtcaacat
      601 cgagaacgcc cgtcaccagt ccaggaaaac ctaattcgcg gaccgtgcgc acatcctcaa
      661 gaatggcggc aaactcaccg tcgctgtagc aaaaatcacc accgcgtggg cgaattatcg
      721 gatgcacagg gatcgtcacc cgctggcgca cggatttcag tacccccaac gacggcgtta
      781 agcccccctc ttttggggct gcgcataatt caactctgtc tgcgccgttt tgctgcgccg
      841 ttagtgcaca ttccatgctg taacagcaaa tttccagtaa tgccattttt gctccttaat
      901 tacgctgact gctcgctggc aacgatctct tcaatggacc acggatgaaa cttaatagtt
      961 gttttgccat cggtgaccgc cagcgtcgga ttcggtaatc gctcatgctc accttttggt
     1021 gaactggttt taactgagat ccccggtagg ctgagtcctt catcagaaag caatgccagc
     1081 gcacgggcat tcagcgtttc tggatcaccc ggcagaacga tttcaatatg ttcccaacct
     1141 tcgtgtgggt aacgtttttc cccgggccac ggtagctcca cattggaaaa ctgccagtgc
     1201 gcaacctgta ccggttcatg caatttaaac agacaaatcg gtctgccatt gatcattttt
     1261 tctgacaaaa gctcgccaca ctgttcaaaa ccgcgacgcc agcgctcagc ggtgacgttt
     1321 tgatggcaac gcaaagaaat gtgatcggca gtcagcggag tgatatccaa ccccagacgg
     1381 cgggaaagtt catctaatgc gtgggtaaat cgcggtaaat ccgatgcaat atcctgcagc
     1441 tcgtcgatag attgccagtt cgccataatc actcttcgtc tttcagtaaa agcgttaatt
     1501 taccctgttg ccctgtgcca accaaccgct gatttcacgc cgcttctgat gcaatagtga
     1561 aaacggcaat acgccacgcg cacgttgctg acgaaaacag ccatttgcag tatactcccg
     1621 ccctaatttc tttaactggt gcgggcaatt tttgctcgct tcatcaatgt aaggtattcc
     1681 ggtgaatatt caggctcttc tctcagaaaa agtccgtcag gccatgattg cggcaggcgc
     1741 gcctgcggat tgcgaaccgc agattcgtca gtcagcaaaa gttcagttcg gcgactatca
     1801 ggctaacggc atgatggcag ttgctaaaaa actgggtatg gcaccgcgac aattagcaga
     1861 gcaggtgctg actcatctgg atcttaacgg tatcgccagc aaagttgaga tcgccggtcc
     1921 aggctttatc aacattttcc ttgatccggc attcctggct gaacatgttc agcaggcgct
     1981 ggcgtccgat cgtctcggtg ttgctacgcc agaaaaacag accattgtgg ttgactactc
     2041 tgcgccaaac gtggcgaaag agatgcacgt cggtcacctg cgctctacca ttattggtga
     2101 cacagcagtg cgtactctgg agttcctcgg tcacaaagtg attcgcgcaa accacgtcgg
     2161 cgactggggc actcagttcg gtatgctgat tgcatggctg gaaaagcagc agcaggaaaa
     2221 cgccggtgaa atggagctgg ctgaccttga aggtttctac cgcgatgcga aaaagcatta
     2281 cgacgaagat gaagagtttg ccgagcgtgc acgtaactac gtggtaaaac tgcaaagcgg
     2341 tgacgaatat ttccgcgaga tgtggcgcaa actggtcgac atcaccatga cgcagaacca
     2401 gatcacttac gatcgtctca acgtgacgct gacccgtgat gacgtgatgg gcgaaagcct
     2461 ctacaaccca atgctgccag gaattgtggc ggatctcaaa gccaaaggtc tggcagtaga
     2521 aagcgaaggg gcgaccgtcg tattccttga tgtgtttaaa aacaaggaag gcgaaccgat
     2581 gggcgtgatc attcagaaga aagatggcgg ctatctctac accaccactg atatcgcctg
     2641 tgcgaaatat cgttatgaaa cactgcatgc cgatcgcgtg ctgtattaca tcgactcccg
     2701 tcagcatcaa cacctgatgc aggcatgggc gatcgtccgt aaagcaggct atgtaccgga
     2761 atccgtaccg ctggaacacc acatgttcgg catgatgctg ggtaaagacg gcaaaccgtt
     2821 caaaacccgc gcgggtggta ccgtgaaact ggcagacctg ctggatgaag ccctggagcg
     2881 tgcacgtcgt ctggtggcag aaaagaaccc ggatatgcca gctgacgagc tggaaaaact
     2941 ggctaacgcg gttggtattg gtgcggtgaa atatgcggat ctctccaaaa accgcaccac
     3001 ggactacatc ttcgactggg acaacatgct ggcgtttgag ggtaataccg cgccatacat
     3061 gcagtatgct tacacgcgtg tattgtccgt gttccgtaaa gcagaaatta acgaagagca
     3121 actggctgca gctccggtaa tcatccgtga agatcgtgaa gcgcaactgg cagctcgcct
     3181 gctgcagttt gaagaaaccc tcaccgtggt tgcccgtgaa ggcacgccgc atgtaatgtg
     3241 tgcttacctg tacgatctgg ccggtctgtt ctctggcttc tacgagcact gcccgatcct
     3301 cagcgcagaa aacgaagaag tgcgtaacag ccgtctgaaa ctggcacaac tgacagcgaa
     3361 gacgctgaag ctgggtctgg atacgctggg tattgagact gtagagcgta tgtaatcgat
     3421 ttttcgtgag agtgaagcct gatcagggtt agccgatcag gcttttttat tgccatctaa
     3481 atgtattctg aaaacggaca tgccattgtt ttctcactgt tgagtaagag gccagaagcg
     3541 taatatccag gcccaggaaa acgataacgg ttcaatttaa ggaataccga agtgtttaaa
     3601 tttcttgtat taacattagg cattatctct tgccaggctt acgcagaaga tacggttata
     3661 gtaaacgacc atgacacttc agccatcaaa gattgttggc aaaaaaattc agatgatgat
     3721 actgacatta acgtgatcaa atcatgcctg cgacaagaat acaatctcgt cgatgcgcaa
     3781 ttaaataaag cctatggtga agcttatcgt tatatagaac aagtgccacg cacaggtgca
     3841 aaaaaacttg atactgaaca acttaacttg cttaaaaaat cacagcgagc ctggctggat
     3901 tttagggaca aagaatgtga attaatcctt tcaaatgagg atgttcagga tttaagtgac
     3961 ccctattctg aatcagaatg gctatcatgt atgatcatac agaccaatac gcgaactcgc
     4021 cagttgcagc tataccgtaa ctctgaagat ttttatccaa gccctttgac aagaggataa
     4081 ttcacatctt tttggcatgt tttgttgcaa gctattcctg ataaatgatt gcaacaaaac
     4141 atccagcctt ttgactgagt tattaaacat actcgcgagc gcgtaatttt ttgtctttca
     4201 gcgataattc acaatcactt cattccgttg tacctttagc ggtggaatta accgccctga
     4261 accgccccgg gtttcctgga gagtgtttta tctgtgaact caggctgcca gatcatcgtt
     4321 tccgatggaa gcataataag ctttttctgc ttctgccgga ggagt
//
LOCUS       DAZPEU010000252         4354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPEU010000252 DAZPEU010000000
VERSION     DAZPEU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.254"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            468..3776
                     /gene="icsA"
                     /locus_tag="ACHU2L_003856"
                     /gene_synonym="virG"
     CDS             468..3776
                     /gene="icsA"
                     /locus_tag="ACHU2L_003856"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR1358494.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3920..4238
                     /locus_tag="ACHU2L_003857"
                     /pseudo
     CDS             3920..4238
                     /locus_tag="ACHU2L_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1326 a          744 c         1022 g         1262 t
ORIGIN      
        1 aacataataa aatggaatgt tattcttctc tttaagaaaa cgatacattt ccactaaaaa
       61 caattatgca gttattgcat tggagagtct ggtaggaaag ttgtgatatg aatacattca
      121 tgttattggc tttggggatt aacatcaagg ggcagataga accattgggt gtatagaaaa
      181 acaaagtggt taaactttgg gcaagcgtac agaaagaact gaaaagttgc ggtctgaagc
      241 agactatcat agtaaaatta ttaatttatt atcgaacata tagctttccc cctctttttt
      301 tcaaagcaag acacaggtaa atttctcccg ttgcattgat atataacaca gctctcatgt
      361 tttggttgag gctttgttta atatgttttt gcatatatcg tccctttatt ccggataaaa
      421 cggaatcttt tcaggggttt atcaaccact tactgataat atagtgcatg aatcaaattc
      481 acaaattttt ttgtaatatg acccaatgtt cacagggggg ggccggagaa ttacctacgg
      541 taaaggaaaa aacatgcaaa ttgtcttttt ctccttttgt tgttggtgca tccctgttgc
      601 tcggggggcc aatagctttt gctactcctc tttcgggtac tcaagaactt catttttcag
      661 aggacaatta tgaaaaatta ttaacacctg ttgatggact ttctcccttg ggagctggtg
      721 aagatggaat ggatgcgtgg tatataactt cttccaaccc ctctcatgca tctagaacta
      781 agctacggat taactctgat attatgatta gcgcaggtca tggtggtgct ggtgataata
      841 atgatggtaa tagttgtggc ggtaatggtg gtgactctat taccggatct gacttgtcta
      901 taatcaatca aggcatgatt cttggtggta gcggcggtag cggtgctgac cataacggtg
      961 atggtggtga ggctgttaca ggagacaatc tgtttataat aaatggagaa attatttcag
     1021 gtggacatgg tggcgatagt tatagtgata gtgatggggg gaatggaggt gatgccgtca
     1081 caggagtcaa tttacccata atcaacaaag ggactatttc cggtggtaat ggaggtaaca
     1141 attatggtga gggtgatggc ggtaatggag gtgatgccat cacaggaagc agcctctctg
     1201 taatcaataa gggcacgttc gctggaggca acggaggtgc tgcttacggt tatggttatg
     1261 atggctacgg tggtaatgct atcacaggag ataacctgtc tgtaatcaac aatggagcta
     1321 ttttaggcgg taatggtgga cattgggggg atgctataaa tggtagcaat atgaccattg
     1381 ctaatagcgg atatataatt tcaggtaaag aagatgatgg aacacaaaat gtagcaggta
     1441 atgctatcca catcactggt ggaaacaatt cattaatact ccatgaaggt tctgtcatta
     1501 ctggtgatgt acaggttaac aattcatcca ttctgaaaat tatcaacaat gattacactg
     1561 ggaccacacc aactattgaa ggtgatttat gtgctggtga ttgtacaact gtttcactat
     1621 caggtaacaa attcactgtt tcaggtgacg tttcttttgg tgagaacagt tctttaaatt
     1681 tagctggaat cagtagtctg gaagcttctg gaaatatgtc atttggcaac aatgtaaaag
     1741 tggaggctat tataaataac tgggcgcaga aggactataa actgctaagt gcagataaag
     1801 ggataacagg tttcagtgtt tctaatatat ctatcatcaa tccgttactc actactggtg
     1861 ctattgacta tacaaaaagc tatatcagtg accagaataa attgatctac ggtttgagct
     1921 ggaatgatac agatggcgac agtcatggag agttcaatct gaaagaaaac gctgaactta
     1981 ctgttagtac tattctggca gataatctca gccatcataa tataaatagc tgggacggaa
     2041 aatccctaac aaaatcaggg gagggaactc tcattttggc ggaaaaaaat acctactctg
     2101 gtttcaccaa catcaatgca ggcattctaa aaatggggac agttgaagct atgacacgta
     2161 ccgctggtgt tattgttaat aaaggtgcta ccttgaattt ttcaggcatg aaccaaactg
     2221 ttaacacttt attaaatagt gggactgtgc taatcaataa tattaatgcc ccttttttgc
     2281 ctgaccccgt cattgtcaca ggtaacatga ctctggagaa aaacggtcat gttattctca
     2341 ataatagttc gtcaaatgtc ggtcagacct atgttcagaa aggtaattgg catggaaagg
     2401 gcggaatatt atctttgggc gcggttctcg gcaatgacaa cagtaaaact gaccggctgg
     2461 aaattgcagg ccatgcgtct ggtattacct atgttgcagt gacaaatgag ggaggctctg
     2521 gagataaaac tcttgaaggt gttcaaatta tttcgacaga ttcttctgat aagaatgctt
     2581 ttattcagaa aggccgtatt gttgctggta gttatgacta tcgcctgaaa cagggcactg
     2641 tatctggact gaataccaat aagtggtatc taactagtca gatggataat caagaatcaa
     2701 aacagatgag caatcaagag tctactcaaa tgagtagtcg ccgagctagt tcacagcttg
     2761 tatcttcact taatttgggt gaaggtagta ttcacacatg gcgccctgaa gctggcagtt
     2821 atattgctaa cctgatagca atgaacacga tgtttagtcc ttctctctat gaccgacacg
     2881 gtagcactat tgttgatcct actacaggtc agctcagcga aaccaccatg tggattcgta
     2941 ctgttggtgg acataatgag cataatttag ctgatagaca attaaaaacc acagctaaca
     3001 ggatggttta tcagattggt ggagatattt tgaagacaaa cttcactgat catgatggct
     3061 tgcatgtggg tattatggga gcttatggat atcaggatag caaaactcat aataagtata
     3121 ctagttatag ttcacgagga actgtgagcg gttatactgc cggtttgtac agttcttggt
     3181 ttcaggatga aaaagaacga acaggtctat atatggatgc ttggttgcag tacagttggt
     3241 ttaataatac agtcaaagga gatgggttaa ctggtgagaa atattccagc aaaggaataa
     3301 caggagcttt ggaagctggc tatatctacc caaccatacg ctggactgct cataataata
     3361 ttgacaacgc attgtatctc aatccacaag tccagataac taggcatggg gtaaaagcaa
     3421 acgactatat tgaacacaat ggcactatgg tcacatcctc tgggggcaat aatattcaag
     3481 caaaattggg attgcgtaca tccttaatta gtcagagttg tatcgataag gagactcttc
     3541 gtaagttcga accatttttg gaagtgaatt ggaaatggag ctcaaagcaa tatggtgtaa
     3601 ttatgaatgg catgtcaaat caccagatag gcaaccgtaa tgtgattgaa ctcaaaactg
     3661 gtgtgggggg gcgtcttgca gataacctaa gcatctgggg aaacgtatct cagcaattgg
     3721 gtaataacag ttacagagac acccaaggta ttttgggtgt gaaatatacc ttctgataat
     3781 aaaaatgcag ggcgtgttga tgtcctgcat ttctctggga cccaccgtgt aaaagctcgg
     3841 tgggtttttg tgcacatgat ggtatgcctg cgatcaatgc caaacgtgtt taccagatca
     3901 tgcgccagaa tgcgctgttg cttggcgtta cacaggaatt gcattagaaa gcgatgaacg
     3961 aatgctaccg ccaaatattc gtggttatgc gcctcagatt actggtattg cagaaactaa
     4021 tgcacgtgtt gttgtatctc aacaaggtcg agtaatatat gactctactg taccagctgg
     4081 aactttttct attcaggact tgagtagttc tgtacgagga atattggatg ttgaaatttt
     4141 tgagcaaaat ggtaaaagaa aacatttcca agtagaaatg tgcaggtgtg cctttcttat
     4201 ccagacttgg tcagagtagg tataaattag taacaggtct acctaaaaca aataataaag
     4261 caactggtga tgcatttttt tcggtgaaga tcctacgggg tcctgagccc ggtgaagtgg
     4321 cgaggcaaat cacatgggga agcgtacagc ctga
//
LOCUS       DAZPEU010000253         4310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPEU010000253 DAZPEU010000000
VERSION     DAZPEU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.255"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHU2L_003858"
     CDS             <1..118
                     /locus_tag="ACHU2L_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358495.1"
                     /translation="NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(115..381)
                     /locus_tag="ACHU2L_003859"
     CDS             complement(115..381)
                     /locus_tag="ACHU2L_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR1358496.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="ACHU2L_003860"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="ACHU2L_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1358497.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1045 a         1132 c         1123 g         1010 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaacttc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg
//
LOCUS       DAZPEU010000254         4269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPEU010000254 DAZPEU010000000
VERSION     DAZPEU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.256"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(32..867)
                     /gene="aadA1"
                     /locus_tag="ACHU2L_003861"
                     /pseudo
     CDS             complement(32..867)
                     /gene="aadA1"
                     /locus_tag="ACHU2L_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(925..1449)
                     /gene="sat2"
                     /locus_tag="ACHU2L_003862"
     CDS             complement(925..1449)
                     /gene="sat2"
                     /locus_tag="ACHU2L_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1358498.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(1544..2017)
                     /gene="dfrA1"
                     /locus_tag="ACHU2L_003863"
     CDS             complement(1544..2017)
                     /gene="dfrA1"
                     /locus_tag="ACHU2L_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1358499.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2349..3326
                     /gene="intI2"
                     /locus_tag="ACHU2L_003864"
                     /pseudo
     CDS             2349..3326
                     /gene="intI2"
                     /locus_tag="ACHU2L_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(3514..3753)
                     /locus_tag="ACHU2L_003865"
     CDS             complement(3514..3753)
                     /locus_tag="ACHU2L_003865"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1358500.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1139 a          993 c          936 g         1201 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttgtc caagataagc
      181 ctgcctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
      241 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
      301 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
      361 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
      421 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
      481 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
      541 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
      601 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
      661 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
      721 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
      781 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
      841 tacttcggcg atcaccgctt ccctcatgat gtttaactcc tgaattaagc cgcgccgcga
      901 agcggtgtcg gcttgaatga attgttaggc gtcatcctgt gctcccgaga accagtacca
      961 gtacatcgct gtttcgttcg agacttgagg tctagtttta tacgtgaaca ggtcaatgcc
     1021 gccgagagta aagccacatt ttgcgtacaa attgcaggca ggtacattgt tcgtttgtgt
     1081 ctctaatcgt atgccaagga gctgtctgct tagtgcccac tttttcgcaa attcgatgag
     1141 actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca acaatgtgtt cgatagaggc
     1201 tagatcgttc catgttgagt tgagttcaat cttcccgaca agctcttggt cgatgaatgc
     1261 gccatagcaa gcagagtctt catcagagtc atcatccgag atgtaatcct tccggtaggg
     1321 gctcacactt ctggtagata gttcaaagcc ttggtcggat aggtgcacat cgaacacttc
     1381 acgaacaatg aaatggttct cagcatccaa tgtttccgcc acctgctcag ggatcaccga
     1441 aatcttcata tgacgcctaa cgcctggcac agcggatcgc aaacctggcg cggcttttgg
     1501 tacaaaaggc gtgacaggtt tgcgaatccg ttgctgccac ttgttaaccc ttttgccaga
     1561 tttggtaact ataatttatg ttagaggcga agtcttgggt aaaaactggc ctaaaattgc
     1621 tggggatttc aggaaagtaa acatcacctt ccggctcgat gtctattgta gatatatgta
     1681 gtgtatctac ttgatcgatc aggcttttgt atatctcccc accacctgaa acaatgacat
     1741 gatccgttat tttctttagg ttggttaaag catctttaat tgatggaaag atcaatacgt
     1801 tctcattgtc agatgtaaaa cttgaacgtg ttacgaccgc atactttcgg ttgggtaatg
     1861 ctcccattga ttcaaaagtc ttgcgtccaa ccaacagcca ttggttatag gtaatagctt
     1921 taaacaggag ctgttcacct ttggcactcc atggaatatc agggccattc ccgataactc
     1981 cattcttcga tatagctacc attagtgata gtttcacaat tcttcctcag aggttaacat
     2041 tttattagta agcatgctcg ttttgtcacc cgctgatgct taccgttaat taatataatc
     2101 acttgtatta aatagacttt ctttaaaaat acaagacact ctgttattac aaatcgtgca
     2161 tgccgtctat cctgcttgca cgatgcacat tatctcaccc aaagaactca taacatttta
     2221 atttatttag cattaaatta aaactctacc ggtaaacaag catctctagg cgtaaaaaac
     2281 aggcactttt ttaaatccca gctagaatag gctgtatagg cagacagttg caagacaagg
     2341 agcgttttat gtctaacagt ccatttttaa attctatacg cacggatatg cgacaaaaag
     2401 gttatgcgct gaaaactgaa aaaacttacc tgcactggat taagcgtttt attctgtttc
     2461 acaaaaaacg tcatcctcag accatgggca gtgaagaggt caggctgttt ttatccagct
     2521 tagcaaacag cagacatgta gccataaaca cgcagaaaat cgctttaaat gccctagctt
     2581 ttttgtacaa caggttttta caacagccgt tgggcgatat tgattatatc cctgcaagca
     2641 agcctagacg gctaccctct gttatctctg caaatgaagt gcaacgcatt ttgcaggtta
     2701 tggatactcg caaccaagtt atttttacgc tgctgtatgg tgcaggtttg cgcattaatg
     2761 aatgcttgcg tttgcgggtt aaagattttg attttgataa tggctgcatc actgtgcatg
     2821 acggtaaggg tgggaaaagc agaaacagcc tactgcccac gcgcctaatc ccagcaataa
     2881 aataactcat tgagcaagcg cggcttattc agcaagacga caacttacaa ggcgtagggc
     2941 catcgctgcc ttttgcttta gatcacaaat acccttctgc ttatcgacaa gcggcgtgga
     3001 tgtttgtctt tccctccagc acgctctgca accacccgta taacggcaaa ttatgccgcc
     3061 atcatctgca tgactccgtt gcgcgaaagg cattgaaggc agccgtacaa aaagcaggca
     3121 tcgttagcaa gcgtgtcact tgtcatacat ttcgtcactc gtttgctacg catctattac
     3181 aagcggggcg tgatattcgc actgtgcaag aactcttagg gcataacgat gttaagacca
     3241 cgcaaatcta tacgcatgtg ttgggtcagc attttgccgg caccaccagt cctgcggatg
     3301 gactgatgct acttatcaat cagtaaaagt agataaatat ctttatgtag ccctgagtag
     3361 cccctgatac tttttatggt gaaaaagata aacaatcata gaggggataa atatgagcca
     3421 gttctaccat tgttcagata agtttaagca agatactgtc aatcacagtg accattcatg
     3481 gtcattacac ttcctccagt tgccagacaa tgcctaacat aaaaacgcaa aagcccaaaa
     3541 gtcagagctt aggtgttcta ggcgttttct aagcgctgaa gaaatacgtc ttaccgtaga
     3601 ttgcgtgcgt ttctgaagca cattttcctt atacatggct ttatcccagg cagcttcatc
     3661 aagctcttgg agtaacaagc cagtaataat gcgacactct cgggtttgta gcgagccgct
     3721 ggtgagatcg gagttatagc ggaaaggggt catttgtaat tatttgtggg tggaggatag
     3781 ttaacttatt attgtagttg gaagaggctg ctttccaaac attagcacct cgtaatgctt
     3841 gttactgtat attaatttat gctcttagcg catgttaatg ggctattaca atgcataatg
     3901 tccacatcaa gataactgtg gcttaacacc tgtacaagcg aaaaaaatca atcacctgtc
     3961 cggaaatacg ggatcactgt aaagtgttgg tgaggcagtc taggaggggg atttaatttg
     4021 ggatactgat gaaaggatta tgatcagttg gatataggat cgtactaagc tgagcggcag
     4081 gttagctaga ttagttaacc agataagtga aatctagttc caaactattt tgtcattttt
     4141 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     4201 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     4261 cagctgccg
//
LOCUS       DAZPEU010000255         4211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPEU010000255 DAZPEU010000000
VERSION     DAZPEU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.257"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            140..1204
                     /gene="ulaG"
                     /locus_tag="ACHU2L_003866"
     CDS             140..1204
                     /gene="ulaG"
                     /locus_tag="ACHU2L_003866"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR1358501.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1312..2067
                     /gene="ulaR"
                     /locus_tag="ACHU2L_003867"
     CDS             1312..2067
                     /gene="ulaR"
                     /locus_tag="ACHU2L_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR1358502.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2064..2813)
                     /gene="yjfP"
                     /locus_tag="ACHU2L_003868"
     CDS             complement(2064..2813)
                     /gene="yjfP"
                     /locus_tag="ACHU2L_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1358503.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2995..3324
                     /gene="bsmA"
                     /locus_tag="ACHU2L_003869"
     CDS             2995..3324
                     /gene="bsmA"
                     /locus_tag="ACHU2L_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR1358504.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3473..3748
                     /gene="yjfN"
                     /locus_tag="ACHU2L_003870"
     CDS             3473..3748
                     /gene="yjfN"
                     /locus_tag="ACHU2L_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR1358505.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3865..>4211)
                     /locus_tag="ACHU2L_003871"
     CDS             complement(3865..>4211)
                     /locus_tag="ACHU2L_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1358506.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
BASE COUNT         1119 a         1054 c         1061 g          977 t
ORIGIN      
        1 ttccccactc aaaatcaaca acaacttccc tggcaaaaag tcaaaaaatg gtcattacct
       61 tcctgaagga atagtgtcag gattaatcaa aaccaatcac atgttgattc gaattgaact
      121 atcatgaagg taaatggcga tgagtaaagt gaaaagtatc acccgagaat cctggatcct
      181 gagcactttc ccggagtggg gtagctggct gaatgaagaa attgaacaag agcaggtcgc
      241 ccctggcaca tttgcgatgt ggtggctagg ctgcaccggg atctggttga aatcagaagg
      301 tggcaccaac gtttgcgttg atttctggtg cggcactggc aaacaaagtc acggtaaccc
      361 gttaatgaaa cagggtcacc agatgcagcg catggctggc gtgaaaaaac tgcagccaaa
      421 cctacgcacc accccgtttg ttcttgatcc gtttgcgatt cgccagatcg acgcggtact
      481 ggcgactcac gatcacaacg atcatatcga cgttaacgtc gctgctgccg tgatgcagaa
      541 ctgtgctgat gacgtaccat tcattggacc gaaaacctgc gttgatttgt ggattggctg
      601 gggcgtaccg aaagagcgtt gcatcgtggt caaaccgggc gatgttgtaa aagtgaaaga
      661 cattgaaatt catgcgcttg acgctttcga ccgtactgca ctgatcaccc tgcctgccga
      721 tcaaaaagcg gctggcgtac tgccagatgg catggacgat cgcgcagtaa actacctgtt
      781 caaaacccct ggcggttccc tatatcacag tggcgattcc cactactcta actattatgc
      841 gaagcacggt aacgaacatc agatcgatgt ggcgttagga tcgtatggtg agaacccgcg
      901 cggtattacc gacaaaatga ccagcgccga tatgctgcgt atgggtgaag ccctgaatgc
      961 gaaagtggtt atcccgttcc accacgacat ctggtcaaac ttccaggccg atccgcaaga
     1021 gatccgcgtg ctgtgggaga tcaaaaaaga tcgtctgaag tatggcttca agccgtttat
     1081 ctggcaggtt ggcggcaaat ttacctggcc gctggataaa gacaacttcg agtaccacta
     1141 tccgcgcggt ttcgatgatt gcttcactat tgaaccggat ctgccgttca agtcattcct
     1201 gtaatctgtc gtttataccg gatagcgtgg agaagttcgc gctatccggc gtagtatttc
     1261 ctggctattt caaatatcat ctaaaaaaat caaattttat cggaatagct catgactgaa
     1321 gcacaaagac atcaaatcct cctggaaatg ctcgcacaat tgggctttgt gaccgttgag
     1381 aaagtcgttg agcgtctggg aatttcgcct gccactgcgc gacgcgatat caataaactt
     1441 ggcgaaagcg gcaaactgaa aaaggtgcgc aatggcgcag aagctattac ccaacagcgc
     1501 ccgcgctgga cgccgatgaa tctgcatcag gcgcagaatc acgatgaaaa agtacgtatt
     1561 gctaaagcgg cctcgcagct ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc
     1621 accgcgtttc tgcttgggcg ggaaatgtgt ggcaagccag tgcaaatcat cactaattat
     1681 ctaccgctgg caaattacct gatcgatcaa gaacatgaca gcgtgatcat tatgggtgga
     1741 cagtacaaca aaagtcagtc catcacttta agcccgcagg gcagcgaaaa cagtctctat
     1801 gccgggcact ggatgtttac cagcggaaaa gggctgaccg cagaagggtt gtataaaacc
     1861 gatatgctga cagcaatggc agagcagaag atgctgagcg tggtagggaa actggtggta
     1921 ctggttgata gcagtaagat tggcgaacgc gcgggaatgc tttttagccg tgccgatcaa
     1981 atcgatatgc tgatcaccgg caaaaatgct aacccggaaa tcctgcaaca actggaagcg
     2041 caaggggtca gcattctgcg tgtttaaaga tgctggcgga aaaatgtcac ggcagcatct
     2101 aacgcctcag gcgtaatgcg gtgacgcacg cctggctgcc atgaacaggt tagcagttta
     2161 tcccgtcccg tctcgcttaa ggcctgctgc aaacgtagtg attcgtcggc aggcacaacg
     2221 tcatcgtcga ggccatgcca cagcagtaga ggtctgtccc caagttgttc caggtggttt
     2281 gtcgcttccc actctgccag cggcgcgaca attttattga attcattctg ctgtgctgcc
     2341 gtttcaggta tcagcggtgg aaacagtgaa cgggcgagcg atgtaaaata gcccgatccc
     2401 atcatgctgg cggtacattt caccgtgggg tggcgagcgg taattcccag tgccgtcatc
     2461 gcgcccatcg acgcgccacc gactgccaga cggtcatcaa gcagccagtt ttcttcggct
     2521 attgccgcac gtaaagtagt gaattcctgc atactttgta gcaagatttg ccagaattga
     2581 tttaaccgcc gcgcttcgtc gccactaaaa cggctaccgt gatcgggcgc atccggcatg
     2641 atcacccgca aaccagcctg cgccagcgca acggcaaaat agctatacac caggctggat
     2701 gaagtaaagc cgtgataaaa aattacgcac ggtaacgggg tatctttttg cccgacagga
     2761 taagcatgaa gaacgggaat atctgccagc tcgcgtgatt ctatttcaat catctgtctc
     2821 tcctttttgt ctggtatgac atgaagaaat ccttagcaca agtttctgct ggcggtatta
     2881 gttcataatg ttgagatgtg ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac
     2941 caggtaacaa ttcgggaaca ttcccccaaa gttaaattaa taaggcacta cactatggtt
     3001 agcaggaaac gtaatagcgt tatttaccgg tttgccagtt tattattggt gttgatgtta
     3061 agtgcctgta gcgcactgca aggtacgcca cagccagcac cgccagtaac ggaccatccg
     3121 caagagattc gccgcgacca gacgcaaggg ttacagcgaa taggtagcgt aagcacgatg
     3181 gttcggggtt ctccggatga cgcattagca gaaatcagag caaaagctgt cgctgcaaaa
     3241 gctgattatt acgttgtcgt catggtggac gaaaccatcg tgacaggaca gtggtattca
     3301 caagccattt tgtaccgtaa ataaatcaga caacttacat agtcagcttt acattgcttt
     3361 gcgtccctat gcgtttacct gtgcacaata aattaccggc tggaagcttg ggcgtttatt
     3421 accgcgatgg aatgccctgc aacatgtggg gaaacgaaaa atggagctga cgatgaaaca
     3481 attacttgcc tcaccctcgc tgcaattagt gacttatcct gcgagcgcca cggcacagtc
     3541 tgcagaattc gccagtgctg actgcgtgac gggcctgaat gaaataggtc agatctcagt
     3601 tagcaatatt tcaggggatc cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga
     3661 acaaggtgcg tcatggtacc gcattattac catgtacgaa gaccagcagc ctgataactg
     3721 gcgcgtacag gcgatactgt atgcatgagt gtcatctaca gcaaatgaac aatgccggat
     3781 gcggcgcgag cgccttgtcc ggcctacaat tttcacatat ttcatttagt taatcgaaac
     3841 cagcgtcgca tcagtcgtat acgcttacac acacactccc cccgtcgccc gcagcaataa
     3901 atcattctgg atctgctctg acaaccgtac gccgccgcgc gtatctaaca tcacctgaca
     3961 ccacgcctgc gccattggcg gagaagcata tttcaacatt tgcgcaccgc agcccagcag
     4021 gaatagctga tgagtaatct ctcgccccag ttcttcagct ggcttacgca gctgctgctg
     4081 taaacgacga accgcgcgat caaaatagcg atcctgccct ttcacttcca caaatgcttc
     4141 cgacaataag tcgtatacgc ccgcttgctt attgagaacg cgcaacacat ccaggcacat
     4201 aatattgccg g
//
LOCUS       DAZPEU010000256         4209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPEU010000256 DAZPEU010000000
VERSION     DAZPEU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.258"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHU2L_003872"
     CDS             complement(<1..209)
                     /locus_tag="ACHU2L_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1358507.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHU2L_003873"
     tRNA            complement(324..399)
                     /locus_tag="ACHU2L_003873"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHU2L_003874"
     tRNA            complement(406..480)
                     /locus_tag="ACHU2L_003874"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHU2L_003875"
     tRNA            complement(597..681)
                     /locus_tag="ACHU2L_003875"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHU2L_003876"
     tRNA            complement(690..765)
                     /locus_tag="ACHU2L_003876"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHU2L_003877"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHU2L_003877"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR1358508.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHU2L_003878"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHU2L_003878"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR1358509.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHU2L_003879"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHU2L_003879"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR1358510.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZPEU010000257         4199 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPEU010000257 DAZPEU010000000
VERSION     DAZPEU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.259"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            85..753
                     /gene="ykgH"
                     /locus_tag="ACHU2L_003880"
     CDS             85..753
                     /gene="ykgH"
                     /locus_tag="ACHU2L_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR1358511.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(996..1691)
                     /locus_tag="ACHU2L_003881"
     CDS             complement(996..1691)
                     /locus_tag="ACHU2L_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR1358512.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1684..3111)
                     /locus_tag="ACHU2L_003882"
     CDS             complement(1684..3111)
                     /locus_tag="ACHU2L_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR1358513.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3122..3841)
                     /locus_tag="ACHU2L_003883"
     CDS             complement(3122..3841)
                     /locus_tag="ACHU2L_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR1358514.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1126 t
ORIGIN      
        1 tttgctgata tttaacccga acattcctga tacagactat taatacaaca ttatctgcga
       61 cagattttat tttctggata tattatgagt gaacaaatca aacaggatat cgatctgatt
      121 gagattttat ttcatctgaa gaaaaagatt cgtgttatcc tttttattat ggctatctgt
      181 atggcaatgg tgctgttgtt tctgtatatc aataaagaca atacaaaagt gatttacagc
      241 cttaaaataa accagacaac gccaggtata cttgttagct gtgatagcaa taataatttt
      301 gcctgtcaga ccacaatgac tgaagatgtt attcagcgaa ttactacatt ttttcagacc
      361 agcccagata tcaagaacag agaaataagg ctggaatggt caggagataa gagagattta
      421 ccaactgctg aagaggaaat atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca
      481 gaataccata atggcaggca agttctcgat gagatacaaa cgccttcagc aattaacagt
      541 gagctttata caaaaatgat atacctgacc aggaactggt cattgtatcc gaacggtgat
      601 ggctgtgtaa ctataagctc accagaaata aaaaataaat accctgctgc catttgcctg
      661 gctctgggat tttttctaag cattgtaatt tctgtaatgt tttgccttgt caaaaaaatg
      721 gtagatgaat accaacaaaa ctctgggcag taataaacag cattctgata gagagtgcct
      781 gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt
      841 ctttttcact gtaaatatcc tggcattact aatgcctgga tatttacaga agagcagcat
      901 aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc
      961 cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat aatcagatac
     1021 accgctttca ccggaccatg aaccccgacg actttgataa gctcaatatc cgccgttgag
     1081 ctgggaccac tgatgatgtt aatacatgac ggcattcgtt caccggcctg cgctttctga
     1141 tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg caggataaaa
     1201 agagaagatt ccgggagcag gctcaatgaa cgcccgcgct cggcggcgga aaaaagaacc
     1261 acgcctcccg attcggttaa accatattca gcatacacga cacccacttt agcctgctct
     1321 gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt gcattcctgc
     1381 tgcaaacgtt cactaatccc caattcctcc agcctcgtgt caccgctaat cacgaccgac
     1441 tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc
     1501 agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc
     1561 tgttggttaa gttgggtaag ccgctcgtta gcatagttgt taagcggcgc atcttctgct
     1621 tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa cgttattcaa aaattcgctc
     1681 cgattatcca ttctttttct cctgcgcctg atgtttctta aaccaactac ggaaactctc
     1741 tccgtcagct tcaggaagat cgcgtgcttc catccagtcg ctaatcgcgc caaatttgag
     1801 tggtgttttg ccgccattga taaaccagct tgccgcatga gcgccggcca tcatcccgac
     1861 tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc gttgctctgc
     1921 ttttgcggtg atcccttttt cagccatcac ccgacgatga cgcaaaatca gttttgacag
     1981 cggaatacgc accggacaca cgctgtcaca agcagtgcat aaagagcagg cgtagggtaa
     2041 atctttaaaa tctttatagc cgccaagtag cggagaaatc accgcaccaa ttggccctgg
     2101 ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag tattcataca
     2161 agccccgcag cgaatacagc gcagcacatc ccgaaattca gaggccagca cctcagaacg
     2221 cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc ccgcttcgcg
     2281 cggccctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac tgcgcgccag
     2341 catggtgatc aatacatcta cctcggcaaa cgtgggggca atacgctcca ttcccatcac
     2401 tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat tggtcaccag
     2461 gcataccgaa ccggtctctg ccaccgcgaa attacagccg gtaataccta tttcagcact
     2521 gaggaaatct tcacggattt tttgccggat gaataaggtc atcgcttcag gcgtttccgg
     2581 cccctcatag cccagacgtt cgtgtagcac tcgacggatc tgatggcgat ctttatgaat
     2641 tgccgggacc acaacatgag atggcggatc ttgatccagc tggagaatat attcacccag
     2701 atcggtttca atcacctgaa tgccagcatc ctgcaacaca tgattgacac caatctcttc
     2761 ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg caacctgtaa
     2821 aatgtagcgg gtagcgtctt ctttggtttt tgcaaaatag acgtgaccgc cgttttgcgt
     2881 cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat gatcacgtat
     2941 ctgggcggcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca ttttttgccg
     3001 atttgcccca atacgctgct gcgcgcttgc caccgctttg cgcatgatcg gatcttcaat
     3061 ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca tctttatatc
     3121 ctcagcggct catcaacact tcagcaatat gcatcacttt gactttctgc ccttcccgtt
     3181 gtaatcgccc actgatgttc agcaggcaac tcacgtcagc accaattaaa tactcagggc
     3241 ggacttccat caggtgcaca accttttctt tcaccatctc gccggatatt tcggccattt
     3301 tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag gtcaacagct
     3361 ccagtccacg cacatttttc agcagcgtaa gtggctcgtc cttcactccc agcttacggg
     3421 ccaggctaca agatgggtga tacacagctc tcccttgcaa actggcacct acatcgacta
     3481 cccctaattt attaacaata aaagaggtga gatcctgcat acgcgcggca accttttcgg
     3541 cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt atggcatagg
     3601 tgcaagagcc agccggtgaa ataatgggat cgtcgttatc ctccagtgcg gcgatcagat
     3661 ttttcatccc tggaatcgct tctttgatat aaccgctatt gatcgcaggc tgaccgcagc
     3721 atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc agcacggagt
     3781 ctcgtgccat tcttgatttc agggcgtcac caatacaggt gacaaagaaa ttgacattca
     3841 caactaatac tccattactt catgcccatt tatgcccgat gtttcccccc caccggggga
     3901 tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc acccctcatt
     3961 tgtatgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat tttatttctt
     4021 tcggcatcgc tcacatttta tggggaaatc gacgatcatt tttggcataa gaaatccatt
     4081 taaaaatggc tacatccctt atacctattc agagctactt attcaggggt tattgagata
     4141 tacactgatg gtgtacaaaa aaatcaccga caataatcac gtatcaagta atagtcgga
//
LOCUS       DAZPEU010000258         4185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPEU010000258 DAZPEU010000000
VERSION     DAZPEU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.260"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..192
                     /locus_tag="ACHU2L_003884"
     CDS             <1..192
                     /locus_tag="ACHU2L_003884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358515.1"
                     /translation="SIQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGIT
                     NIISIILQQAQDYDVAKIT"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHU2L_003885"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHU2L_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR1358516.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHU2L_003886"
     CDS             complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHU2L_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR1358517.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1474..4149
                     /gene="adhE"
                     /locus_tag="ACHU2L_003887"
                     /gene_synonym="adhC"
     CDS             1474..4149
                     /gene="adhE"
                     /locus_tag="ACHU2L_003887"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR1358518.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1022 c          960 g         1035 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcatcggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa
     4081 gatgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
     4141 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttc
//
LOCUS       DAZPEU010000259         4182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPEU010000259 DAZPEU010000000
VERSION     DAZPEU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.261"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHU2L_003888"
     CDS             complement(<1..186)
                     /locus_tag="ACHU2L_003888"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR1358519.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHU2L_003889"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHU2L_003889"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1358520.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACHU2L_003890"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACHU2L_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1358521.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACHU2L_003891"
     CDS             3863..4144
                     /locus_tag="ACHU2L_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR1358522.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZPEU010000260         4168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPEU010000260 DAZPEU010000000
VERSION     DAZPEU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.262"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHU2L_003892"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHU2L_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR1358523.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHU2L_003893"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHU2L_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHU2L_003894"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHU2L_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4168
                     /locus_tag="ACHU2L_003895"
     CDS             3787..>4168
                     /locus_tag="ACHU2L_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR1358524.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAZPEU010000261         4114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPEU010000261 DAZPEU010000000
VERSION     DAZPEU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.263"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..158)
                     /locus_tag="ACHU2L_003896"
     CDS             complement(<1..158)
                     /locus_tag="ACHU2L_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR1358525.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHU2L_003897"
     CDS             complement(308..469)
                     /locus_tag="ACHU2L_003897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358526.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHU2L_003898"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHU2L_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            914..1249
                     /locus_tag="ACHU2L_003899"
     CDS             914..1249
                     /locus_tag="ACHU2L_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1358527.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="ACHU2L_003900"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="ACHU2L_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="ACHU2L_003901"
     tRNA            complement(2161..2237)
                     /locus_tag="ACHU2L_003901"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="ACHU2L_003902"
     tRNA            complement(2339..2414)
                     /locus_tag="ACHU2L_003902"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="ACHU2L_003903"
     CDS             complement(2559..3380)
                     /locus_tag="ACHU2L_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1358528.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="ACHU2L_003904"
     CDS             complement(3395..3751)
                     /locus_tag="ACHU2L_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1358529.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="ACHU2L_003905"
     CDS             complement(3764..>4114)
                     /locus_tag="ACHU2L_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1358530.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAZPEU010000262         4100 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPEU010000262 DAZPEU010000000
VERSION     DAZPEU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.264"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHU2L_003906"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHU2L_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR1358531.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHU2L_003907"
     CDS             complement(1433..1759)
                     /locus_tag="ACHU2L_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR1358532.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHU2L_003908"
     CDS             1894..2235
                     /locus_tag="ACHU2L_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR1358533.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHU2L_003909"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHU2L_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHU2L_003910"
     CDS             complement(2833..3543)
                     /locus_tag="ACHU2L_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR1358534.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHU2L_003911"
     CDS             3651..3956
                     /locus_tag="ACHU2L_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR1358535.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1115 a         1034 c          912 g         1039 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg
//
LOCUS       DAZPEU010000263         4069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPEU010000263 DAZPEU010000000
VERSION     DAZPEU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.265"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHU2L_003912"
     CDS             complement(139..3654)
                     /locus_tag="ACHU2L_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR1358536.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT         1057 a         1027 c          844 g         1141 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatt
//
LOCUS       DAZPEU010000264         4064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPEU010000264 DAZPEU010000000
VERSION     DAZPEU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.266"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHU2L_003913"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHU2L_003913"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR1358537.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHU2L_003914"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHU2L_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR1358538.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHU2L_003915"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHU2L_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR1358539.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT         1086 a         1062 c          889 g         1027 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttcct
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgac
//
LOCUS       DAZPEU010000265         4062 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPEU010000265 DAZPEU010000000
VERSION     DAZPEU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.267"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(32..289)
                     /locus_tag="ACHU2L_003916"
     CDS             complement(32..289)
                     /locus_tag="ACHU2L_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358540.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            745..1896
                     /locus_tag="ACHU2L_003917"
                     /pseudo
     CDS             745..1896
                     /locus_tag="ACHU2L_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            complement(1926..2354)
                     /locus_tag="ACHU2L_003918"
                     /pseudo
     CDS             complement(1926..2354)
                     /locus_tag="ACHU2L_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2869..3912)
                     /locus_tag="ACHU2L_003919"
     CDS             complement(2869..3912)
                     /locus_tag="ACHU2L_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1358541.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT         1132 a          892 c          944 g         1094 t
ORIGIN      
        1 tcaagtagat acatacgcag tcagagtaca cttacttttt attatttcct gcaatggcga
       61 ttacggctat tgacagtaat gaagttatta gtgaaccaag acctaaagta gttccgggac
      121 caacaccgtt gtcgcttacg gttactgaca ggaaactgat tgtaccagcg cttattgttt
      181 gagtaaaacc tgtaatagat gcggcaaatc ctgtttttga accatttatt tgtaatgcga
      241 taaaattggc tattgggtaa taaaaaccat aggttggata atttttcaat ctttgataat
      301 tgatttccga ttatgaaaaa taaaactgag aaggagaaaa taattgagta actaaaagta
      361 ctcaggccat aagtatgcat gaaaattgat ggtgatattg atatgtagca aaatagagaa
      421 ctgagtatta atgagacaag tatagtgccg cagataaact ctttggtcac aaaggatgca
      481 ctgttttgac atattacttt ctcatttgca tgagttatct ttggtgataa taacattatt
      541 ataatactga acagcatcat aacaagaaat ataacattcc agttataata ttcaataatc
      601 aatgcgcgag gtgctactaa tggtattact gcgcttacag atgatatatc agaaagcgcc
      661 cgttttagtt ttaatggagg gtgatcttac ccaccaatag tggacacagg actaagtgag
      721 taaactctca accagaggtg actcatgaca aaaccagtat caatcagcaa gaagccccgt
      781 aaacaacata cgcctgaatt tcgtaacgaa gccctgaaac tcgctgaacg catcggtgtg
      841 gccgccgcag cccgtgaact cagcctgtat gaatctcagc tttatgcctg gcgcagtaaa
      901 cagcagcaac aaatgagttc gtcagagcgc gaaagcgaac tggccgctga aaatgtccgc
      961 cttaaacgac aactggcgga gcaggctgag gaactgtcca tcctccaaaa ggccgcgaca
     1021 tacttcgcga agcgcctgaa atgaagtatg tctttatcga aaatcatcgg gcagagttca
     1081 gcatcaaagc gatgtgtcgt gtacttcggg ttgcccgcag cggctggtat gtctggctca
     1141 ggcgtcgtca ccagatgagc ctgcgccaac agtttcggct cacctgcgat gccgctgtcc
     1201 ataaggcatt ctttgaggca aagcagcgat acggtgctcc ccgccttgct gacgaactgc
     1261 cggagttcaa tattaaaacc attgccgcca gcctgcgtcg tcaggggctg cgggcgaaag
     1321 ccagccggaa gttcagcccg gtcagctacc gtgcacatgg cctgcccgta ttggagaatc
     1381 tgctggagca ggacttcagc gccagtggcc cgaaaccaga agtgggcggg tgacatcacg
     1441 tacttgcgta ccgatgaggg ctggttgtat ctcgcagtag tcatcgacct gtggtcacgc
     1501 gccgtcattg gctggtcgat gtcaccgcga atgacagcac aactggcctg tgatgcactg
     1561 caaatggcgt tgtggcggag aagacgcccg gaaagcgtca ttgttcatac ggaccgtggt
     1621 ggtcaatact gttcagggga ttatcaggca atgctgaagc gacacaacct gcgtggcagt
     1681 atgagtgcga aaggctactg ttatgacaat gcctgtgtgg aaagcttctt tcattcgctg
     1741 aaggtggaat gtatccacgg ggaacgcttt agcagccggg aaataatgcg ggcaacggtg
     1801 tttaattata tcgagtgtga ttacaatcgc tggcgtcgtc acagtgcctg tggcggtctc
     1861 agcccggaac aatttgaaaa ccataatctc gcttagggcc gtgtccacat tacgtgggta
     1921 ggatcagtat gggtcgtctt gtatttgtca tactgcccaa tctcaccaat cgaaaatgct
     1981 ccttttggca aaggttcaag caggtctctg ggaatggagg tcgctggcgt cggatataaa
     2041 aaaccctcac ggcttagcaa ggctggcgtc actatcggtg taacaatatc aggcgtgcct
     2101 gcggaacggt ccttaccatg ctgcagtaac cggaaataaa cttttgggcg taagatgaca
     2161 ttgacagttt cgacatcgtc tttttctccc ttaaaaaatt tattgacaat cgcttcgtca
     2221 agacgtcccg ccgctatccc atgccagtga gtgaaatttt gggcgtcttt ccgttcaaaa
     2281 cagccctttc ctgactcagc atctgcaagc gagttgcgcc agtatgaggc aaaccttaaa
     2341 gcattttcat ccattaccag tcctgtgact caatgaaatc cattgattta tcgaatataa
     2401 aaccaccgta tggacaacta ttgtccatat taaaaaagtt ggatgcaatg ttttgtttat
     2461 aattcgatat caaatgtact ggttgtacga ggttcagaac acaagcacat tcaacagtac
     2521 gtcaggacta ttgatttcat aacagcgaat acaatttttt actgtttata atggctgcct
     2581 gtgttggacg gggataccag ttcaaccgaa aacgccagag gaggggatta cccgctggca
     2641 gggtaaatct gtggtttttg atgccagcta taacgtgttt gacgataaag tattacgaaa
     2701 gaactttaat gagttcatgg agtaattcgt taaagtaatt tacaacgcta tccttttgca
     2761 aaggaaactt tcagaaaata accccagggt aatcacctgg ggaaggaaca cagtgttatt
     2821 attggataat aaattattac ttgttttaaa gttacactaa tgcaatatct actgatactg
     2881 gatgcttgaa tgtttcatcc ctgctgcgga aaagggcaat atccagggcc agatatattc
     2941 accgttcata tttctgcatt tctgtacaat ttcctcagaa agtgtcttgt caattctttt
     3001 ccacaggtgc tcctgctgtc ttaaaatact ttcgcatatt tcgaaatcat cttcctgaat
     3061 cagcatttcg gcgcatttaa tgatttctga tcgttcatgc agcacggagg aatggttata
     3121 gagccagttt acaaacagca atgcttccgg atattgtccc cataatggtg agtatgttgc
     3181 atagtaatct tctgccgtat ccagagagcc ggagaagtca tgccacacag caagtggaaa
     3241 tggatgcaga tttccgcaca gcatttctgc gatgtaagtg tttgtggcaa agccatggcg
     3301 aaaagctgtg gcggccttaa catgttcccc gttcagaaaa tgcaggagac cgagttcata
     3361 ccagtaaggt gaaaattcat cagcatgctc tttcagtaca ctgaaggctc gctcatgatc
     3421 gccggtgcgt aacagctctg aacccaacaa ccagcgagcc ccctgattat cttcaggatt
     3481 gtatgccagt attttatcag tgagcataac cgcatcctga tggcgctgta agtgcacatt
     3541 tgcgagaatt gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat gcatccagat
     3601 tatttcacca cagaaacttt ccgggatgat acgattacct gccgccagtc ctttaagagc
     3661 ggcgttcagt gccttacgcg gggcattctg ttccagaaat gcatatgcca gatgagcgtg
     3721 tatatcaata aagtccggct cccgttgagc aaggcgtcgg agtttgttga tgtaactcgt
     3781 ctcgctgatt tcatcagcgt cgtatgcatc aagcacttct tcaaactccc ccagcaatgg
     3841 gccaaaccag gtatccggaa atacgaaata acccttgtta tcgtgcactt caaagcataa
     3901 tctatcgttc attttctgag cctgtaaaac atgtggtgat atagtctgag agcataaaag
     3961 ttgagattct gtgtatcaag tcctgtcttg gcaccgaatg aatattgctt acgaagcccc
     4021 ttcatgcttt ccttgagaac aataatttta tctttatgtg aa
//
LOCUS       DAZPEU010000266         4042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPEU010000266 DAZPEU010000000
VERSION     DAZPEU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.268"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(126..284)
                     /locus_tag="ACHU2L_003920"
     CDS             complement(126..284)
                     /locus_tag="ACHU2L_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1358542.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACHU2L_003921"
     CDS             complement(886..1077)
                     /locus_tag="ACHU2L_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1358543.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACHU2L_003922"
     CDS             1142..1465
                     /locus_tag="ACHU2L_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR1358544.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACHU2L_003923"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACHU2L_003923"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACHU2L_003924"
     CDS             2143..2673
                     /locus_tag="ACHU2L_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR1358545.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACHU2L_003925"
     CDS             2680..2913
                     /locus_tag="ACHU2L_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR1358546.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACHU2L_003926"
     CDS             complement(3459..3851)
                     /locus_tag="ACHU2L_003926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358547.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZPEU010000267         3983 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPEU010000267 DAZPEU010000000
VERSION     DAZPEU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.269"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(155..775)
                     /gene="fklB"
                     /locus_tag="ACHU2L_003927"
     CDS             complement(155..775)
                     /gene="fklB"
                     /locus_tag="ACHU2L_003927"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1358548.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            994..1539
                     /locus_tag="ACHU2L_003928"
     CDS             994..1539
                     /locus_tag="ACHU2L_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR1358549.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(1523..2170)
                     /locus_tag="ACHU2L_003929"
     CDS             complement(1523..2170)
                     /locus_tag="ACHU2L_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1358550.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            2382..3173
                     /locus_tag="ACHU2L_003930"
     CDS             2382..3173
                     /locus_tag="ACHU2L_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR1358551.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            3183..>3983
                     /locus_tag="ACHU2L_003931"
     CDS             3183..>3983
                     /locus_tag="ACHU2L_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR1358552.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT          987 a         1016 c          998 g          982 t
ORIGIN      
        1 agtgagtgtc atatcacggt gataacgcga aacagctcac aaaaatacac gcttacgggg
       61 tctttgcaac acaaaatagc gccgtttggc aaactgaagg gtttattgct gaatgcctgc
      121 tcccctctgg ttcgagggga acagaatgcg ctgcttagag gatttccagc agttcgactt
      181 caaacaccag ggtgctgaac ggagggatgg atgcacctgc gccgcgctcg ccatatgcca
      241 gttcctgcgg gatagtcagt tcccatttag aacctaccgg catcagagtc agtgcttcaa
      301 tccagccagg gatcacgcca ttaaccggga attcagcggg ttcaccacga gcaacggagc
      361 tgtcaaacac ggtgccgtcg atcagtttac cggtgtaatg aacacgaacg cggtcggtac
      421 gtgccggaat tgcgccttca ccctggttga tcacgcggaa ttgcaggcca gattcggtgc
      481 tattcacacc ttcttttttg gcgttttctt ccaggtattt cacgccttca gcagccatcg
      541 cctggaaacg ctgacgacga acggcatcgg cgcgttcgtg gatttcacgc agcgcgcgat
      601 gcaccacatc aaccggcaca gccggatgtt tgccttccag cgcatcggca atacctgcaa
      661 ccagtgcttc tggcagcagc ccttccaggc cagattcact cagttgttgc cctacctgca
      721 aaccaatgcc gtagcttgct tgcgcttcga tggtgtcaaa agttggggtg gtcatcgtat
      781 ttcctttctt agggtataaa agtagcgggc agcatatcag ccacacttct ttgggtaaaa
      841 ctttgtcgca ggaatgatga caaatcccgg ggaaaaagaa acaatagcaa tatcccgtat
      901 gaatataata aaggcgggtt taccacggac atcatagcgt tagcttattc ataggctatg
      961 attgaggaac aagacgtgga gcaggaggaa accatgcccg ggcgctttga actaaaacca
     1021 accctggaga aagtctggca cgcgccggac aattttcgct tcatggaccc gctgccgccg
     1081 atgcatcgtc gggggatcat tattgccgcc attgtattgg tggtcggatt tctgcttcca
     1141 tctgatgata cgcccaacgc gtcggtggtg acgcgtgaag cgcagttaga tattcagtcg
     1201 caatcacaac cgccgacaga agaacagccg caaactaccc aaacgcaacc attccagcca
     1261 gatagcggaa ttgataatca atggcgctct taccgtgtcg aacccggtaa aacgatggcg
     1321 cagctgttcc gcgaccacgg actgcccgct accgatgtct atgcaatggc gcaggtagaa
     1381 ggcgcgggta agccgctgag taatttgcaa aatgggcaga tggtgaaaat ccgccagaac
     1441 gccagcggcg tggtgacggg cttaaccatt gatacgggga ataatcaaca ggtcctgttt
     1501 acccgccagc cggatggcag ttttattcgt gcgcggtaga tgaagggtca accaaaagcc
     1561 agctaaggat tgtctctttg atgaccttac gtctggcttc cagtgcctgg ggggaaacca
     1621 tactgatatg ataaactagg ccaagcgtat gctggttgat caaataatat cccccaagcg
     1681 cagcaatatt aatattcacc tgcaatgggt cgatacctgg cttaaaaata ttgtatttct
     1741 tcccttcatc caataaggat tccattaact gcaagtgagc atggttaatt tctaagagac
     1801 gctgtgattt tgcataatgc acacctttac tttgattttc actatgcaca atttttaaaa
     1861 accaaggatt agcaatataa taatcccatg taaaatcaat aattttctca atggcttttt
     1921 gtggcggaag atctgagaaa tcaagggcct tttctttttg tcgaatatcg ttccaggtat
     1981 attccagcac ttcgataaat aaattttctt tattaccaaa gtggtgataa accgtctgtt
     2041 tactacaccc tgctgccttt acaatattat ctactctggc cccttcataa ccatattcgg
     2101 caaattcatt aaccgcacat agcagcaatt tttccttcag gctctgtacg ttgctggtat
     2161 caatatacat aacgcttccc cacgacctct tcgtggtgac tatcataacc tgatttgatt
     2221 tcagcttcag aaaaaagggc aaaaacttgt actttcatca ggttatcaaa tgtcctgtga
     2281 tcgccatcgc atatcaacca actagatggt ttatcttttt atcctccagt cgcaagctaa
     2341 tcatcaccaa ggaaacaaaa ttcaaaaaag agagctattg tatgaaatcc actcgtcctg
     2401 ttgccgtgat taccggagcc actcgcggta ttggaaaggg ctgtgcgcag gagcttgctc
     2461 gtggcgggtt taacctgctg attaacgatc gtcccgatgc cgacagcgta gaaaaactgc
     2521 atatcacaca acaagaatgt ttggcggaag gcgtggaagt gatttgcttc cctgctgatg
     2581 tcggtgatct atctctgcat gaggagatgc tcgacgcggc gcaaaaccag tgggggcggc
     2641 tggactgtct gctcaataac gcggggattt cagtgaaaaa acgcggtgac ctcctcgacc
     2701 tcgaaccaga cagcttcgat caaaacattg ccattaacac ccgcgcgccg ttcttccttg
     2761 ctcaggcatt cagtaagcgc ttactggcac agccgaaacc ggaagcggag ctgccgcacc
     2821 gctcgattat tttcgtcagt tccatcaacg ccatcatgct ggcgatgaat cgcggcgaat
     2881 acaccattgc caaaaccgcc gtttctgccg ctgctcgtct gtttgctgct cgcctttgta
     2941 atgaacaaat tggcgtttat gaagtgcgcc ctggtctcat caaaactgat atgactattc
     3001 cggcaacggc ttattacgac gaactgattg ccaaaggtct ggtgccgtgg ggacgttggg
     3061 gttatccggc tgatattgct tccaccgtcc gcgcgatggc ggaaggtaaa ctgatttaca
     3121 cctgtggtca ggcggtcgca atcgacggcg gcttaagtat gccgcgcttc tgaggctgaa
     3181 tcatgagaga tctgttacaa cgcccggatt tattcagtat caataccgcg acactgggct
     3241 ataaaacgcc gctacccgcc attattgatg cctgcgccgc acgcggtatt ggcgcaattg
     3301 ccccctggcg cagggagttg cagggtgaag atctccagca aatcacccgc cagcttgccg
     3361 ccagtaacat gagcgtttcc ggtttgtgcc gtagcactta ctacaccgcg ccgacactgg
     3421 cggaacgcaa actggcgatt gatgataacc gccgggcgct ggacgacgca gcggtgctta
     3481 acgccgcctg ttatatgcag gtggttggcg gtctgccaac gggcactaaa gatctatatg
     3541 aagcgcgaga acaagtgaaa caagggattc gccagttgct gccacattcg aaagatgtcg
     3601 gcgtgccaat tgcgctggag ccactgcacc cgatgacagc tgccgatcgc tcttgcctgt
     3661 gcacgttacg tcaggcgctg gactggtgtg atgaactcga tcccgatggt gagtttggcc
     3721 tcggcgtggc ggtggatgtt tatcacgtct ggtgggatcc ggatctcgcc agtcagatcc
     3781 tgcgtgccgg aaaacgcatc ctcgcgtttc atgtttccga ctggttaatg ccgaccactg
     3841 atctggtcaa tgaccgtggt atgccgggag atggcgtcat caatattccg tcaattcgtc
     3901 ggctggttga aaacgccgga tttaatggtg ctattgaact ggaaatattc tcgccttact
     3961 ggtggcagaa agatattaac agc
//
LOCUS       DAZPEU010000268         3955 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPEU010000268 DAZPEU010000000
VERSION     DAZPEU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.270"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(302..1951)
                     /gene="pgi"
                     /locus_tag="ACHU2L_003932"
     CDS             complement(302..1951)
                     /gene="pgi"
                     /locus_tag="ACHU2L_003932"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR1358553.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2188..2384
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2476..3825
                     /gene="lysC"
                     /locus_tag="ACHU2L_003933"
     CDS             2476..3825
                     /gene="lysC"
                     /locus_tag="ACHU2L_003933"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR1358554.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT          930 a          986 c         1038 g         1001 t
ORIGIN      
        1 aataacaata aaaatcataa gcttaaaata aaaaacaaaa gtaattaatc tgcgtattct
       61 ccgaaataaa caacgattaa aaatctattt taggattaac ctcacggtat gatttccgtt
      121 aaatcacaga caaggggatt tatctgataa aacaatgccg acttattgtc ggcattgttt
      181 ttaaggttat ttaactcctc atactatgca ttaatgtcga tattggcatc aggctccggt
      241 tgccggatgc ggcgtaaacg ccttatccgg cctacatatc ggcatcaggc acgcatgatg
      301 attaaccgcg ccacgcttta tagcggttaa tcagaccatt ggtcgagcta tcgtggctgc
      361 tgatttcttt atcatctttc agctctggca gaatacggtt tgccagttgt ttacccagct
      421 ccacgcccca ctggtcgaag gtgaagatgt tcaggatcac gccctgagta aagattttgt
      481 gctcatacag cgcaatcaac gcacccaggc tgaacggagt gatttcgcgc agcaggatgg
      541 agttggtcgg gcggttacct tcgaacactt tgaacggcac cacgtagtca agcgttgccg
      601 gatctttacc ctgatcgcga tattcctgct caaccacttc gcgggattta ccaaacgcca
      661 gcgcttcggt ctgggcgaag aagttagaca gcagtttctg gtggtgatca gagagcgggt
      721 tatgggtgat agccggggcg atgaaatcgc acggtaccat tttggttccc tggtggatca
      781 gctggtagaa cgcgtgctga ccgttagtgc ctggttcacc ccagataatc gggccagtct
      841 ggtaatccac aacgttaccg ttacggtcaa catacttacc gttggactcc atattgccct
      901 gctggaagta cgccgcgaaa cggtgcatat actggtcata cggcagaatc gcttcagttt
      961 ccgcaccaaa gaaattgttg taccagatgc caatcagcgc cagcaggaca ggcaggtttt
     1021 tctcggcagg cgtggtggag aaatgcttgt ccattgcgtg tgcgccagaa agcagttcaa
     1081 cgaagttatc aaagccgatg gagagaacaa tcgacaggcc aatcgctgac cacaaagagt
     1141 aacggccgcc gacccagtcc cagaactcga acatgttggc agtatcaata ccaaactcgc
     1201 caacggcttt ggcattggtt gagagcgccg caaaatgttt cgcaacgtgt ttttcatcac
     1261 ctgccgcttt caggaaccag tcacgcgcgc tatgggcgtt ggtcatggtt tcctgagtgg
     1321 tgaaggtttt agatgctacc aggaacagcg tggtttccgg gtttactttt ttcagcactt
     1381 ccgagatgtg agtcccatcg acgttagaaa caaagtgcat gttcaggtgg tttttgtacg
     1441 gacgcagtgc ttcggtcacc atgtacgggc cgaggtcaga accgccgatc ccgatgttca
     1501 ctacgtcagt gattgctttg ccggtataac ccttccactc accggagata atcgcttccg
     1561 agaaggtttt catcttctcc agcaccgcgt tgacttctgg cataacatct ttgccatcaa
     1621 ccagaatcgg ggtattgcta cgattacgca gcgctacgtg cagcacggcg cggttttcag
     1681 tgcggttgat cttctcgcca gagaacatcg acttaatcgc gcccgccaga tcgcactctt
     1741 tcgccagatc ctgtaatttc gccagcgtct cttcagtgat gcggtttttg gagtaatcca
     1801 ccagcatctg atcgtcgaag gttgcggaga acttagaaaa acgatcgccg tctttagcaa
     1861 aaagatcggc gatcgtaacg tctttcattt catcgaagtg tttctgtagt gcctgccagg
     1921 cagcggtctg cgttggattg atgtttttca ttagcaatac tcttctgatt ttgagaattg
     1981 tgactttgga agattgtagc gccagtcaca gaaaaatgtg atggttttag tgccgttagt
     2041 gtaatgttga gtgtaaaccc ttagcgcagt gaagcattta ttagctgaac tactgaccgc
     2101 caggagtgga tgaaaaatcc gcatgacccc gtcgttgaca accgccccgc tcacccttta
     2161 tttataaatg tactacctgc gctagcgcag gccagaagag gcgcgttgcc caagtaacgg
     2221 tgttggagga gccagtcctg tgataacacc tgagggggtg catcgccgag gtgattgaac
     2281 ggctggccac gttcatcatc ggctacaggg gctgaatccc ctgggttgtc accagaagcg
     2341 ttcgcagtcg ggcgtttcgc aagtggtgga gcacttctgg gtgaaaatag tagcgaagta
     2401 tcgctctgcg cccacccgtc ttccgctctt cccttgtgcc aaggctgaaa atggatcccc
     2461 tgacacgagg tagttatgtc tgaaattgtt gtctccaaat ttggcggtac cagcgtagct
     2521 gattttgacg ccatgaaccg cagcgctgat attgtgcttt ctgatgccaa cgtgcgttta
     2581 gtagtcctct cggcttctgc tggtatcact aatctgctgg tcgctttagc ggaagggctg
     2641 gaacctggcg agcgattcga aaaactcgac gctattcgca acatccagtt tgccattctg
     2701 gaacgtctgc gttacccgaa cgttatccgt gaagagattg aacgtctgct ggagaacatt
     2761 actgttctgg cagaagcggc ggcgctggca acgtctccgg cgctgacaga tgagctggtc
     2821 agccacggcg agctgatgtc gaccctgctg tttgtcgaaa tcctgcgcga acgcgatgtt
     2881 caggcacagt ggtttgatgt acgtaaagtg atgcgtacca acgaccgatt tggtcgtgca
     2941 gagccagatg tagccgcgct ggcggaactg gccgcgctgc agctgctccc acgcctcaat
     3001 gaaggcttag tgatcaccca gggatttatc ggtagcgaaa ataaaggtcg tacaacgacg
     3061 cttggccgtg gaggcagcga ttatacggca gccttgctgg cggaggcttt acacgcatct
     3121 cgtgttgata tctggaccga cgtcccgggc atctacacca ccgatccacg cgtagtttcc
     3181 gcagcaaaac gcattgatga aatcgcgttt gccgaagcgg cagagatggc aacttttggt
     3241 gcaaaagtac tgcatccggc aacgttgcta cccgcagtac gcagcgatat cccggtcttt
     3301 gtcggctcca gcaaagaccc acgcgcaggt ggtacgctgg tgtgcaataa aactgaaaat
     3361 ccgccgctgt tccgcgcgct ggcgcttcgt cgcaatcaga ctctgctcac tttgcacagc
     3421 ctgaatatgc tgcattctcg cggcttcctc gcggaagttt tcggcatcct cgcgcggcat
     3481 aatatttcgg tagacttaat caccacgtca gaagtgagcg tggcattaac ccttgatacc
     3541 accggttcaa cctccactgg cgatacgttg ctgacgcaat ctctgctgat ggagctttcc
     3601 gcactgtgcc gggtggaggt ggaagaaggt ctggcgctgg tcgcgttgat tggcaatgac
     3661 ctgtcaaaag cctgcggcgt tggcaaagag gtattcggcg tactggaacc gttcaacatt
     3721 cgcatgattt gttacggcgc atccagccat aacctgtgct tcctggtgcc cggcgaagat
     3781 gccgagcagg tggtgcaaaa actgcatagt aatttgtttg agtaaatact gtatggcctg
     3841 gaagctatat ttcgggccgt attgattttc ttgtcactat gaggtagcct gagtttaacg
     3901 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaa
//
LOCUS       DAZPEU010000269         3948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPEU010000269 DAZPEU010000000
VERSION     DAZPEU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.271"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            184..387
                     /locus_tag="ACHU2L_003934"
                     /pseudo
     CDS             184..387
                     /locus_tag="ACHU2L_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            406..3792
                     /locus_tag="ACHU2L_003935"
     CDS             406..3792
                     /locus_tag="ACHU2L_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1358555.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
BASE COUNT          975 a          918 c         1248 g          807 t
ORIGIN      
        1 ctggtcgttg taaatattgc taattgctta atattattag tgattatgac tggaaagata
       61 aagtgtgctg taaagcggtg atgggtatta cattcatgat taaaaaaaca gaacctgtca
      121 ataaataatt cctttattaa aacggactat ttacattatg acggaatatt atctcaacga
      181 ggcggtttgg aaatacagag ccagtttctc cggggtaacg agcaggtgta ccagtgtcag
      241 gtggcatttg tattgccggg tgagcgggtc atgatggcct tcacctacgc cagaaccaca
      301 ccgctgaccc cggcagatat gacgcgctgg gctgaaatta aaaagaatct gcgcttcagg
      361 atgcggcagg aagtcaggac gaattaatca tacagggaac gggacatgga agaggcattc
      421 tgggcggcaa gacagggtga tgccctgctg catacgtcgt ttatggcaga tgtgctgggg
      481 gcggtgctgg aggtggccgc aaatgtcgtt attgatgccc tcattgtcgg cacaacctcg
      541 ttactggcga cgtcagtggg gatcacgatg gggtgcacgg ccgtcgtgct gaccggtttt
      601 gtggcgggtg tggcgatggt gtacaccggc gtgacagaaa aggtcagcga agcctgttca
      661 gccctcgcaa atatgctttt cccgcctcag attgaaggtt acatcctcac cggctctggt
      721 gacacatgga tcaacagcaa acctgccgcg cgggcagcgg cgactgcggc atcgcggcag
      781 gacatagagg cgcaggaagc gcaggcgaag gcagaacagg aagaagcgca gcgtcaggag
      841 gatgccagaa cgttccggga tgtggccggg gaatatctgg aaatggcggg ggccatagcc
      901 ctggcggtta acccggtgac agggcctgtg ctgatgtccc gggctctgca cgaccagctc
      961 agtacggaag aagggcgttc tgaaatggtg gatggcgtga cacattttgt gtcggaacta
     1021 tggcagccca ctgtggccag tgcggccccg ggatccaccc cgacccctga cgataaaatc
     1081 gactgccata aacatccttc ctcactgacg cagtttctcg cacaaaaaaa agccgccttt
     1141 ctggatgccc ccctgggaac ggcccttaac ctgctgaatc cgggcggtat gctggaaacc
     1201 gcctttcagg gagtgaatgc ggttattggc agcatcagta atctgttcaa aggcgatgac
     1261 gagccgcccg cagcggaata tatcgcggaa ggcgcgcggg atgtgcgtat caacagccag
     1321 ccggcgacac gcagcggggc acgctgtacc tgcgaggcca gggtggtgaa tgagccgggg
     1381 aacggggcgt ttgtgtcacc ggatgtgcgc attggcggtc cgttactggt ggtcagggat
     1441 atcagaagcg gcaggtcgca gataaccctg gtggcaaccg tggcgctgat gtttcttcgc
     1501 ccgggcaaaa tgctttctaa aatcgcctgt tttgctgcca gtgtcgggat gagcatgctg
     1561 acgcagaagg ccaccagtgc ccttccgcat ccggtcaatg ccgccaccgg ggccaaatac
     1621 ctggccgatg acgatgactt cgatttcagt ctgccgggcc actttccgct ggactggcag
     1681 cgcgtgtaca gcagtcgtga cgaacgtacg gaaggaatgt ttggtcaggg ctggagtgta
     1741 atgtatgaag tcagcctcgt gtgcacaccg ggtagcgcag acgaaaactg catgaccttt
     1801 gtgtcgggca tggggcgacg cttggatatg gaagccgtac tgccgggggg cgggttttac
     1861 agccccggtg aagggctggc ggtaaggcgt ggtgagcagg ggcactggct tatcagcagc
     1921 gatgacgggc agtgttttct gtttgaggct gacccgcacc acccgcagcg ccagcggctg
     1981 aaaatgctgg gcgaccgtaa cagcaactgt ctgaatctgt actacgacga ccggggccgc
     2041 attgtggaaa tcagcggcga acagcagcgt ccgtgcatcc gtctgtatta cgaactggcg
     2101 gcgcatcccc ggcgggtgac acagatttat cagcatttcc ctgagactgc acctttactg
     2161 ctgcgtcgtt acagctatga cgaggccggc cacctgaacg gcgtgtacga cagcacgggg
     2221 cacctgctgc gtgagtttgc ttacgacgaa aaccactgca tgacgctgca ccgccagccg
     2281 ggcggcgaag ggtattatta ccagtggggc tggtatgaag gtccggatga cgcggcatgg
     2341 cgggtgacgg ggcaccacac ggacagcggt gaacagtacc ggctggcgtg ggacctggcg
     2401 cagcgcaggc tgtgcgtgac ggacggactg gggcgcacac gttatcacca gtgggatgcg
     2461 cagaatcagg tgacggcgta ccaggacgaa gccggacagg tgacgacatt ccgctggagc
     2521 gacgaagaac gactgttgct ggggatgacc gaccctcagg gcggcaaatg gcgctatgtg
     2581 tatgacctgc aggggcatat cacggagacg cacgacccgc tggggcgggt ggagcaggcg
     2641 cagtggcacc cggtgtggca ccagccggaa acggaggtgg acgcggcggg aaacctgctg
     2701 gggaggcgcg cgggggaaag ggccacggca gaaaacagcg tggtgccgtt caaccggctg
     2761 atgtcttacc gtggtgtgca ctaccgttat gacgaatttg gccgtgcggt ggaaaaggag
     2821 ggccggagcg gaacgcagag ttaccgttat gatgcggaac accggatggt ggaagtgacg
     2881 acagcgcggg ggacataccg gtatgtgtac gatgcactgg ggaggcggac ggaaaaacag
     2941 cacatcagcc ctgacggtaa gccgtacaac cggacaaaat ttttatggga cggaatgcgg
     3001 ctggcgcagg agagcaggcc ggagggaaca ggcagcctgt acatctacag ggaccagggg
     3061 agttatgaac cgctggcgcg ggtggataag gcgggcaaag agggcccgaa ccggatactg
     3121 tattttcaca cggatgtgaa cggcgcgccg gaagagatga cggacagcga cgggaaaatc
     3181 gtgtgggaga cggggtatca ggtctggggt aacacgatac aggagaagga ccacggcggg
     3241 gtggagcaga acctgcgcta ccagggacag tacctcgaca gggaaacggg gctgcactac
     3301 aatctgcaca ggtactatga tccggatgtc gggcggttca tggtaactga cccgataggg
     3361 ttgagaggcg gtttaaatct ctacagttat gcacctaatc ctctaaaata tgctgatcca
     3421 cttggattaa ctccatgtgc tgttagtaac caaaaagcga atcgtttatt agatagtagt
     3481 gaaaccaaag ttactgttag gtctagaagt gacgctgaac aattgtttat ggatagatat
     3541 ttaggtcata attataaaaa tatgacaggt gagtcaggcc ccagcactaa gaacttaatg
     3601 gaatatctta ctgaaaataa aacaaaagca ggttcatatc attgggatga tataaaagat
     3661 ccttctgtaa caaaaccttc atatagagtg tcaggtcatg ggccaggaaa tcctgatggt
     3721 gatttaccac atttacaagt tcatcaacat ggtggaagtg ttagacatat atttttcccg
     3781 tgggagacat aataatgaac aatctcaata cattattaag tggaaaggtt ttgctcaaag
     3841 aaattagtcc attaatgcct ggatatagaa catggattga aatttcattg atcgatgaat
     3901 taaaaccatc ttatcctttt agattagatg aatatgctat gatagggc
//
LOCUS       DAZPEU010000270         3931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPEU010000270 DAZPEU010000000
VERSION     DAZPEU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.272"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            72..302
                     /locus_tag="ACHU2L_003936"
     CDS             72..302
                     /locus_tag="ACHU2L_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358556.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            280..780
                     /locus_tag="ACHU2L_003937"
     CDS             280..780
                     /locus_tag="ACHU2L_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR1358557.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            780..2198
                     /locus_tag="ACHU2L_003938"
     CDS             780..2198
                     /locus_tag="ACHU2L_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR1358558.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            2198..3046
                     /locus_tag="ACHU2L_003939"
     CDS             2198..3046
                     /locus_tag="ACHU2L_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR1358559.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            3046..3501
                     /locus_tag="ACHU2L_003940"
     CDS             3046..3501
                     /locus_tag="ACHU2L_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR1358560.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            3504..>3931
                     /locus_tag="ACHU2L_003941"
     CDS             3504..>3931
                     /locus_tag="ACHU2L_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR1358561.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY"
BASE COUNT         1034 a          957 c         1013 g          927 t
ORIGIN      
        1 gttggtctgc ctaaccagac cgcgtgataa ccagagggag cttcggctcc cgtttttatc
       61 tggagacaag catgacacac atgatctttc gtcatggcga catgaagaag tggaaaggcg
      121 ttggctacga ctttgaaatc gtgaaagccg aagagcttca ggaatatctg gatgctggct
      181 ggttttcaca tcctgatgac cttttgaagg atgttgcaga gccagagcca gaagaaaaac
      241 agcgtaaaaa gcctggtcga aaacctaagg cggcagcaga tgaacctgac aacgaagggt
      301 gatttagttc ttgcggcatt acgtaagctc ggtgttgcat caaatgccac gttaaccgat
      361 gtcgaaccgc agtctatgga agacggcgtc aacgaccttg aaatgatgat ggctgaatgg
      421 cttggcggtg atgcgtcacc tggtatcaac gttggctaca tttttgctga tgcagatgtt
      481 gctccggatc cgggcgatga gcacggttta tcaaataacg ctatcaatgc cgtcattttc
      541 aaccttgcct gccgaattgc tccagattat gcgctggaag cgtctgcaaa acttataacc
      601 actgccagat acgggaaaga gcgactcgtc aaactgtctg caatggacag ggcaaaagca
      661 gctaaatgta agtccggtta tccaaaccgt atgcctgttg gtagtggtaa ccagttggcg
      721 aagtggaatg gttggaatta cttccaccga aaggaacctt gcgataacgg gagcgaataa
      781 tgcctattca gcaacttccg cttatgaaag gtgtcggcaa agacttccga aacgccgact
      841 atatcgacta tctgccagtg aatatgctgg ctacacccaa agaaatcctg aacagcagcg
      901 gatatcttcg ctcattcccg ggcattgcca aacgttctga cgtgaacggt gtatctcgcg
      961 gcgtcgagta caacatggcg cagaatgctg tttatcgcgt gtgtggcggc aagctgtaca
     1021 aaggagaaag tgaagtcggt gatgttgccg gaagtggtcg tgtatcaatg gcgcatggtc
     1081 ggacatcaca ggcggtaggc gttaatggtc aactggtcga gtatcgctat gatggcacgg
     1141 tcaaaaccgt ctcaaactgg cctacagaca gtggattcac tcagtatgag ttaggttcag
     1201 ttcgcgacat tacgcgctta cgtgggcgtt atgcgtggtc aaaagacgga actgattcat
     1261 ggtttatcac tgaccttgaa gacgaatctc atcctgaccg ttacagcgca caatatcgtg
     1321 ccgagtctca gcctgacggc atcatcggta tcgggacatg gcgagacttc atcgtctgct
     1381 ttggttcatc gactattgaa tatttctccc tgactggcgc aaccaccgtt ggtgccgctt
     1441 tgtatgttgc acaaccatcg ctgatggtgc agaaaggcat tgccggaact tactgcaaaa
     1501 cgccattcgc tgattcgtat gcgtttatca gcaatccggc aacgggtgcg ccgtctgtgt
     1561 atatcatcga ctccggtcag gtgtcaccaa ttgccagcgc gagcattgag aaaatcctcc
     1621 gctcctacac tgctgatgaa ctggctgatg gtgtgatgga atcgctgcga tttgatgctc
     1681 atgagttgct gattatccac cttccgcgtc acgtcctcgt gtacgacgca tcttcaatcg
     1741 ccaatggccc acaatggtgt gtgctgaaaa caggtctgta tgacgatgtg taccgcgcta
     1801 tcgacttcgt ttacgaaggc aatcagataa cgtgcggcga taagctggaa tctgttaccg
     1861 ggaaactgca attcgatatc agcagccagt acgacaagca acaggaacac ctgctgttta
     1921 ctccactctt caaagcggat aacgccagag tgttcgacct tgaggttgaa tcgtcaactg
     1981 gcgttgcgca gtacgccgac cgcctttttc tctctgcaac cactgactgc atcaattacg
     2041 ggcgtgagca gatgattgag cagaatgaac cgttcgttta cgacaagcgt gttttgtgga
     2101 agcgagtagg gcgcatcagg aaaaatgtcg gcttcaaatt gcgcgttatc acgaagtcac
     2161 ctgtcactct gtctggcgct cagataagga ttgagtaatg gctgattcga atctcaatgt
     2221 gccggtaatc atccaagcta cgcggctcga tacatcagtc cttccacgca atatcttctc
     2281 gcagtcgtat ctgctttacg ttatcgcaca gggcactgat gttggtaacg tggctaacaa
     2341 agccaacgag gccggacagg gcgcttatga tgcacaggtc aggaacgatg agcaggatgt
     2401 gattctcgct gaccatgagc agcgaatttc tgctgcggaa gcaacgcttg ttaatcatga
     2461 ggagcgaatc agacaggcag aatcaactct tcaggaccat gaaacacgaa tagctcagaa
     2521 tgaaagcgat atttcgtcgc ttgacacaag agttcagtcg ctggagtcgc aggtttcaga
     2581 ccatgaaacg cgcatcgatg ctctggagta tgcaaccaca cgcaagaagt cagaggttgt
     2641 ttactctggt gtatctgtaa ccatcccgac agcgccgacc aaccttgtta gcctgctgaa
     2701 aacgctcaca ccgtcatccg gctcgttggc accattcttc gacaccgtta acaacaagat
     2761 ggttgtgttc aacgagaaca aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc
     2821 cagcggaacc gccaacaggt caatgcagct aaccttttcc ggctctgttc ctgacacact
     2881 ggtcagcagt cgtaatgcgg cgacaacaac cgacaacatc ctgttagcta cgttcttcag
     2941 cgtggataaa gacggctttc ttgccacaaa tggcagtacg ttaaccattc agtcgaatgg
     3001 tgcggcgttt actgccacaa ccatcaagat aatcgcggag cagtgatgat tacattcaaa
     3061 ccaacgcgaa acatcgacct gatagaagca gtcggaaatc accctgacat tatcgccggg
     3121 agcaacaacg gtgatggata cgactacaaa cctgaatgcc gttactttga ggtgaacgtg
     3181 cacggtcagt ttggcggcat tgtttactat caggagattc agccgctgac attagattgc
     3241 cacgccatgt acctgccaga gattcgcggc ttcagcaagg aaattgggct ggcgttctgg
     3301 cgatacattc tgactaacac caccgttcag tgcgtaacat cgttcgctgc acgcaaattc
     3361 cgacacggtc agatgtactg cgcaatgatt ggccttaagc gtgtaggaac catcaagaaa
     3421 tacttcaaag gcgtggatga cgtgactttt tacagcgcca cacgcgaaga actaatcgac
     3481 ttcctgaatc acgggagata gccatgttat atgcatttaa gctgggcaga aaactgcgcg
     3541 gcgaggaacc ttattgccct gaaaaaggcg ggaaaggtgg cagttctgat aaaagcgcaa
     3601 agtatgcagc agaagcccag aagtatgctg cagacctgca aaatcagcag tggcagacga
     3661 tcatgaaaaa ccttgctccg ttcacgcctc ttgcggagca gtatgttaac cagcttcaga
     3721 acctttccag tttagaaggt caggggcagg cacttaatca gtattacaac tctaagcagt
     3781 ataaagacct tgcagggcag gcgcgttacc agagtcttgc tgctgcggag gcgacgggtg
     3841 gacttggttc gacagccaca agcaatcaac tggctacgat cgcgccgaca ctcggtcagt
     3901 cttggttatc aaatcagatg agcaattaca a
//
LOCUS       DAZPEU010000271         3876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPEU010000271 DAZPEU010000000
VERSION     DAZPEU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.273"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1183
                     /gene="ppk1"
                     /locus_tag="ACHU2L_003942"
     CDS             <1..1183
                     /gene="ppk1"
                     /locus_tag="ACHU2L_003942"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR1358562.1"
                     /translation="NFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFDAIRER
                     DVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHNGKKVT
                     VVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVVRYAHI
                     GTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQNSRRL
                     LYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGMCSLIP
                     NLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEVATPLL
                     DPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIKSLEQP
                     E"
     gene            1188..2729
                     /gene="ppx"
                     /locus_tag="ACHU2L_003943"
     CDS             1188..2729
                     /gene="ppx"
                     /locus_tag="ACHU2L_003943"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR1358563.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(2768..>3876)
                     /gene="pdeF"
                     /locus_tag="ACHU2L_003944"
     CDS             complement(2768..>3876)
                     /gene="pdeF"
                     /locus_tag="ACHU2L_003944"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1358564.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT          995 a          966 c          959 g          956 t
ORIGIN      
        1 taattttaaa gactttatta atttccccaa tgtcggcaaa gccaatctgg tgaacaaacc
       61 actgccgcgt ttacgccata tttggtttga taaagcccag ttccgcaatg gttttgatgc
      121 cattcgcgaa cgcgatgtgt tgctctatta tccttatcac acctttgagc atgtgctgga
      181 actgctgcgt caggcttcgt tcgacccgag cgtactggcg attaaaatta acatttaccg
      241 cgtggcgaaa gattcacgca tcatcgactc aatgatccac gccgcacata acggtaagaa
      301 agtcactgtg gtggttgagt tacaggcgcg tttcgacgaa gaagccaaca ttcactgggc
      361 gaagcgcctg accgaagcag gcgtgcacgt tatcttctct gcgccggggc tgaaaattca
      421 cgccaaactg ttcctgattt cacgtaaaga aaacggtgaa gtggtccgtt acgcacacat
      481 cgggaccggg aactttaacg aaaaaaccgc gcgtctttat actgactatt cgttgctgac
      541 cgcagatgcg cgtatcacca acgaagtacg gcgggtattt aactttattg aaaacccata
      601 ccgcccggtg acatttgatt atttaatggt gtcaccgcaa aactctcgcc gtctgttata
      661 tgaaatggta gaccgcgaaa tcgccaacgc gcagcaaggg ctgcccagtg gtatcaccct
      721 gaagctaaat aaccttgtcg ataaaggcct ggttgatcgt ctgtatgcgg cctccagctc
      781 cggcgtaccg gttaatctgc tggttcgcgg aatgtgttcg ctgatcccca atctggaagg
      841 cattagcgac aacattcgtg ccatcagtat tgttgaccgt taccttgaac atgaccgggt
      901 ttatattttt gaaaatggcg gcgataaaaa ggtctacctt tcttccgccg actggatgac
      961 gcgcaatatt gattatcgta ttgaagtggc gacaccgctg ctcgatccgc gcctgaagca
     1021 gcgggtgctg gacatcatcg acatattgtt cagcgatacg gtcaaagcac gttatatcga
     1081 taaagaactc agtaatcgct acgttccccg cggcaatcgc cgcaaagtac gggcgcagtt
     1141 ggcgatttac gactacatca aatcactcga acaacctgaa taaccctatg ccaatacacg
     1201 ataaatcccc tcgtccgcag gagtttgctg cggtcgatct tggttcaaac agttttcaca
     1261 tggtcatagc ccgtgtggta gatggtgcca tgcagattat tggccgcctg aaacagcggg
     1321 tgcatctggc ggacggcctg gggccagata atatgttgag tgaagaggca atgacgcgcg
     1381 gtttgaactg tctgtcgctg tttgccgaac ggctacaagg gttttctcct gccagcgtct
     1441 gtatagtcgg cacccatacg ctgcgtcagg cgctgaacgc cactgacttt ctgaaacgcg
     1501 cggaaaaggt cattccctac ccgattgaaa ttatttccgg taatgaagaa gcccgtctga
     1561 tttttatggg cgtggaacat acccaaccgg aaaaaggtcg caaactggtt attgatattg
     1621 gcggcggatc tacggaactg gtgattggtg aaaatttcga acctattctc gttgaaagcc
     1681 gccggatggg ttgtgtcagc tttgcccagc tttattttcc tggcggggtc atcaataaag
     1741 agaattttca gcgcgctcgc atggcggcag cacaaaaact ggaaacttta acctggcaat
     1801 tccgtattca gggctggaac gttgcaatgg gcgcttccgg taccataaaa gccgcccatg
     1861 aagtgttaat ggaaatgggc gagaaagacg ggataattac cccggaacgt ctggaaaaac
     1921 tggtaaaaga aattttacgt caccgtaatt tcgcatcgct gagtttaccg ggtctttccg
     1981 aagagcggaa aacagtcttc gttcctggac tggcgatttt atgtggtgtg tttgatgctt
     2041 tagccatccg tgaactgcgc ctttctgacg gggcgttacg cgaaggcgta ctgtatgaaa
     2101 tggaaggacg tttccgtcat caggatgtgc gtagtcgcac cgccagcagc ctcgccaacc
     2161 agtatcacat agacagcgaa caggcccgac gagtgctgga taccactatg caaatgtacg
     2221 aacagtggcg ggaacagcaa ccgaagctgg cgcatccgca actggaggcg ctactgcgat
     2281 gggccgccat gctgcatgag gtcgggttga atatcaacca cagcggtttg catcgccact
     2341 ccgcttatat tctgcaaaac agtgacttgc cgggttttaa tcaggaacag cagctgatga
     2401 tggcgacact ggtgcgctat caccgtaaag cgattaagct cgacgatctg ccgcgcttta
     2461 ccttgtttaa gaagaaacag ttcctgccac tgatacagct attgcgcctt ggcgtattac
     2521 taaacaatca acgtcaggca accaccacac cgccaacatt gacactgatt actgatgaca
     2581 gtcactggac actgcgtttc ccgcatgact ggtttagtca gaatgcgctg gtactgcttg
     2641 atctggaaaa ggagcaagaa tactgggaag gcgtggctgg ctggcggttg aaaattgaag
     2701 aagaaagtac cccagaaatc gccgcttaat gctttgcggg ccgacgagaa atgtcggccc
     2761 gcattattca ggcactttcg cgaatgggtt cgatttcatt cagcgtatca attaacggtt
     2821 gcggcttacc aataagataa ccctgcatat aatcgatccc caaagagagc accgcctcgc
     2881 ggatctcttc gttttcaacg tactctgcca ctacccgcat tttcttcatt cgcgccagat
     2941 ggcaaatcga cgccacaatc tgataatcca gactatttga cacaatattg cggataaagc
     3001 tgccgtcaat tttaagcaga tcggcattca catttttaag ccgtgcatag ctggcgtagc
     3061 cggtgccgaa atcatcaatc gcaatctggc agcctaattc ctgaagatgc tgcaaggtga
     3121 tttgcgcctg cttaacattg gtcagagcat tactttcggt gacttcaaaa ataagttgcc
     3181 acgcttcaat ctgatattta gccagcaact gactgacttc aacaggaaaa cgagcctgac
     3241 ataccgaggt tggagacaga ttaatagcaa aacggtgagc gggcatcttc gctctgtttt
     3301 cagccataaa ttgcagcgta tgctcaatga cccacatgtc gatactcgac gataaaccaa
     3361 attcgtgcgc gaccggcaag aagctaccgg ggccgatcag ttcatcattc tcacctttca
     3421 tgcgcagaag aatttcatgg taaacatcac cacgcatacc ggtaatcggc tgggccatca
     3481 ggaaaaaatg gttgtgttcc agcgcctgct gtagtcgatt catcatcgcg actttatctt
     3541 tcaattcgcg ttgcaaatac attgccccgc aacgctgcat attttccggg gcgttggtca
     3601 cgatggaaag ttcggcgacc gtatttagct ctcccagcag caggtagata tgattcactg
     3661 gcgagcgcac atagcagtaa ctgacgccaa tctgcggttg catcggcatc ccatcccaaa
     3721 agaaacgaaa ttgcttgaga tgactatcca gtgcggtaat gcgctcctgg tgcgattctg
     3781 tattcagtcg cagcgcgaga tcgttacccg aaagctgata aacatcttca cccggttcca
     3841 gcaagggtga cagccagtga gaaagttttt gcttgt
//
LOCUS       DAZPEU010000272         3861 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPEU010000272 DAZPEU010000000
VERSION     DAZPEU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.274"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..578)
                     /gene="yibB"
                     /locus_tag="ACHU2L_003945"
     CDS             complement(<1..578)
                     /gene="yibB"
                     /locus_tag="ACHU2L_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFR1358565.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFM"
     gene            882..1814
                     /gene="rfaD"
                     /locus_tag="ACHU2L_003946"
     CDS             882..1814
                     /gene="rfaD"
                     /locus_tag="ACHU2L_003946"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR1358566.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1824..2870
                     /gene="rfaF"
                     /locus_tag="ACHU2L_003947"
     CDS             1824..2870
                     /gene="rfaF"
                     /locus_tag="ACHU2L_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR1358567.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2874..>3861
                     /gene="rfaC"
                     /locus_tag="ACHU2L_003948"
     CDS             2874..>3861
                     /gene="rfaC"
                     /locus_tag="ACHU2L_003948"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR1358568.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEI"
BASE COUNT         1025 a          901 c          952 g          983 t
ORIGIN      
        1 atcatgaaat caaaagcctg ttgttgagat gtaacagtaa gccctttttt tatagtgaaa
       61 agatgcatct tattttcatc aaatgggaaa tcccaaattt tcaatcctcg agttacattt
      121 ggcttacggc aatagccaaa atctatccac gcaacaagag gcgttttcac taaacccatg
      181 ttgatagctt tatttacaaa ataggcctta aggttacata ccaaaacata ctctggtgac
      241 cagtactctg ggttttttaa ttgtcgaggc tcaagtctat ttgtaaatga ctcatctttt
      301 tgaatttttt cgatacgact tctgatatat ctaaatttct tttttatatc gataacgata
      361 accgttgttg gttttccgtt gcgaatcgct tcaatccttg gtttcaaatc gggagaagtg
      421 aaaataatca tctcattttc aagtgcggcc agacgctcaa agtaactaaa ataaacatca
      481 actgaacgag ctagtttttc acggaaccct ttattagcag tccaatcccc tctaccaatg
      541 tcaaaatatg cagtgataat tgtggtggat gatttcatta ctaataagac cattaatata
      601 aaaaaagaat tatatcataa atattaacag aatgtatact gtatattatc cattcaaatg
      661 attccgttat ttatcataca tcggaatatt gatactaaag cactattcac atgcaaaacc
      721 aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt agcatccttg
      781 cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca gacattcgtg
      841 tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc gttaccggcg
      901 gcgcgggctt tatcggcagc aacatcgtta aggccctgaa tgataaaggc atcaccgata
      961 ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg gatctggata
     1021 tcgcggacta tatggataag gaagacttcc tgatccagat tatggctggc gaagagttcg
     1081 gtgatgtcga agcgattttc cacgaaggcg catgctcttc caccaccgag tgggatggca
     1141 agtatatgat ggataacaac tatcaatact ccaaagagct gctgcactac tgtctggagc
     1201 gcgaaatccc gttcctgtat gcctcttccg cagccaccta cggcggacgc acctccgact
     1261 ttattgaatc ccgcgagtac gaaaaaccgt tgaatgtcta cggttactca aaattcctgt
     1321 ttgatgaata tgttcgtcaa atcctgccag aagcgaactc gcagattgtt ggcttccgct
     1381 atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc
     1441 atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt agcgagaact
     1501 tcaaacgtga cttcgtctat gtgggcgacg tggcagatgt aaacctgtgg ttcctggaaa
     1561 atggcgtttc cggtatcttc aacctcggca ccggtcgtgc agaatccttc caggcagtag
     1621 cagatgctac gctggcttat cacaagaaag gccagatcga atacattccg ttcccggata
     1681 agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg cgcgcggcgg
     1741 gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg gcctggctga
     1801 atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct tgggttggcg
     1861 acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc caggcgataa
     1921 tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg gaagttaacg
     1981 aagctatccc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg
     2041 gtcatagcct gcgtgaaaag cgctacgacc gcgcctacgt cttaccaaac tccttcaaat
     2101 ctgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc
     2161 gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg
     2221 aacgctatgt cgcgctggcc tatgacaaag gcattatgcg taccgcacaa gatctgccgc
     2281 agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc
     2341 aattttcgct ttcatcagaa cgtccgatga ttggcttttg cccgggtgcg gagtttggtc
     2401 cggcaaaacg ctggccacac taccactatg cggaactggc aaagcagctg attgatgaag
     2461 gttatcaggt ggttctgttt ggctctgcga aagatcatga agcgggcaat gagattcttg
     2521 ccgctttgaa taccgagcag caggcatggt gtcggaacct ggcgggggaa acacagcttg
     2581 atcaagcggt tatcttgatt gcagcctgta aagccattgt cactaacgat tctggcctga
     2641 tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtcca agtagcccgg
     2701 acttcacacc gccgctatcc cataaagcgc gcgtgatccg tctgattacc ggctatcaca
     2761 aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac attactcccc
     2821 agcgcgtact ggaagaactc aacgcgctat tgttacaaga ggaagcctga cggatgcggg
     2881 ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc gcactcactg
     2941 atgcccagca ggcaatccca gggattaagt ttgactgggt ggtggaagaa gggttcgcac
     3001 agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata cgtcgctggc
     3061 gtaaagcctg gttctcggcc cccataaaag ctgaacgcaa agcgtttcgt gaagcgctac
     3121 aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc gcggcactgg
     3181 tgacacgtct ggcgcatggc gtaaagcatg gattggactg gcaaaccgct cgcgaacctt
     3241 tagccagcct gttttacaat tgtaagcatc atattgcaaa acagcagcac gccgtagaac
     3301 gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc cagggcgatt
     3361 atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat
     3421 ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg cgagaattga
     3481 ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg
     3541 aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg ccgaagatga
     3601 gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg
     3661 ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat ggaccaaccg
     3721 atccgggatt aattggtggg tatgggaaga atcaagtaga gtgtcgttct acaagtatgt
     3781 ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa ataataacca
     3841 ataagttgac atcggagata a
//
LOCUS       DAZPEU010000273         3841 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPEU010000273 DAZPEU010000000
VERSION     DAZPEU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.275"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            81..1415
                     /gene="adeQ"
                     /locus_tag="ACHU2L_003949"
                     /gene_synonym="yicO"
     CDS             81..1415
                     /gene="adeQ"
                     /locus_tag="ACHU2L_003949"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR1358569.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1468..1920
                     /locus_tag="ACHU2L_003950"
     CDS             1468..1920
                     /locus_tag="ACHU2L_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR1358570.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2131..3321
                     /gene="nepI"
                     /locus_tag="ACHU2L_003951"
     CDS             2131..3321
                     /gene="nepI"
                     /locus_tag="ACHU2L_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR1358571.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3362..3655)
                     /gene="yicS"
                     /locus_tag="ACHU2L_003952"
     CDS             complement(3362..3655)
                     /gene="yicS"
                     /locus_tag="ACHU2L_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR1358572.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT          784 a          860 c         1018 g         1179 t
ORIGIN      
        1 atgtgataat tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat
       61 ctcctccaat ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag
      121 ggacgctttc gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga
      181 ttgcggggat gaccactttt ttaaccatgg tgtacattgt ttttgtgaac ccgcaaatcc
      241 tcggcgcggc acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta
      301 tcggcagtat tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg
      361 ggctgaacgc cttctttgcg ttcgtggtcg tgggggcgat ggggatctcc tggcagaccg
      421 ggatgggcgc gatattctgg ggcgcaattg ggctattttt gctcacgctg tttcgtatcc
      481 ggtactggat gatctccaac attccattaa gtttacgtat tggtatcacc agtggaatcg
      541 gattatttat tgccttaatg ggattaaaaa acactggtgt tattgtcgcc aataaagaca
      601 cgctggtgat gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt
      661 ttattataac cgtgttgtca tcacgtcatt ttcatgccgc ggtgctggtt tctattgtgg
      721 tgacgtcttg ctgtggatta tttttcggtg atgttcattt tagcggcgtc tattccattc
      781 cgcctgatat tagcggcgtc attggtgaag tagatttgag cggcgcgtta acacttgaac
      841 tcgccggtat cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa
      901 ttggtgtaac tgataaagcg ggcttaatag atagcaacgg taaattcccc aatatgaata
      961 aggcgctgta tgttgatagc gtcagttcgg tggcaggcgc atttatcggt acctcgtctg
     1021 ttactgccta tattgaaagt acttctggtg tggcagtcgg tggtcgcacg gggctaacgg
     1081 ctgtcgtggt cggcgttatg ttcctgttag ttatgttctt ctcaccgctg gtggcgatgg
     1141 ttcctcctta cgcaaccgct ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc
     1201 tggcgcgcgt taactgggat gattttaccg aatcggtgcc tgcgtttatt accacggtga
     1261 tgatgccctt tactttctcg atcaccgaag ggattgcact cggctttatg tcgtactgca
     1321 tcatgaaagt gtgcaccggg cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag
     1381 ctctgttcgc attgaagatt attctggtgg attagtaact ggcgagggtt tccagatatc
     1441 atgagttctg attacgcagg agaactcatg atctggataa tgctcgccac gctggcggta
     1501 gtgtttgtgg ttggttttcg ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc
     1561 agcgatcggc tgaatatcga tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag
     1621 tcagccggtg acgaattttt acgttatttg catcgcccgg atgagtcgca cctgcaaaac
     1681 gccgcgcagg tgttgcttat ctggcaaatt gtcattgtcg atgatagcga acagaacctg
     1741 ctgcaatggc accggatttt acaaaaagcc cgcctttccg cgccgattac cgatgctcag
     1801 gtcaggctgg cgctgggttt tctgcgtgaa accgaacctg aaatgcagga tattaatgct
     1861 tttcagatgc gctataacgc gttctttcag cctgccgagg gcgttcactg gttgcattga
     1921 aaccgtttcg tcttatcagc ctacagaccg cgcctgacct gtcgggtgga taagacgttc
     1981 acgccgcatc cggtaatctt tgccctgttc cgtttcgatc ttaactccag ctcaagcaac
     2041 accgtaaaag ttgcaaaaac gttaaattcg tcacacattt caatgttaaa gtggtcggct
     2101 tttcccctga aacatgccac gggtaacacc atgagtgaat ttattgccga aaaccgcggc
     2161 gcggatgcca tcacccgacc gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc
     2221 tgtctgatta tcgttgagtt tttgcccgtc agtttgttga cgccaatggc ccaggattta
     2281 ggcatttcgg aaggggttgc cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt
     2341 gccagtttgt ttattaccca gacaattcag gccactgacc gccgctacgt tgttattttg
     2401 tttgccgttt tgctgacgct ctcctgcttg ctggtttcct ttgctaactc attcagtttg
     2461 cttttaatcg gtcgtgcctg tctggggctg gcgctgggcg ggttctgggc gatgtcggcg
     2521 tcgctgacca tgcgtctggt accgccgcgt acggtgccga aggcgctgtc ggtgatcttc
     2581 ggcgcggttt ctattgcgct ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt
     2641 atcggttggc gcaatgtctt taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg
     2701 attatcaaat cattgccttc actgccaggc gaaccctcgc atcagaaaca aaatactttc
     2761 cgcttattac aacgtccggg tgtgatggca gggatgatcg ccatcttcat gtctttcgcc
     2821 gggcagtttg ctttcttcac gtatattcgc ccggtgtata tgaacctggc gggattcggc
     2881 gtggatggct taacgctggt gctgttgagt tttggtatcg ccagctttat tggtacgtcg
     2941 ctttcgtcgt tcattcttaa acgttcggta aaactggcct tagcaggcgc gccgttaata
     3001 ctggctgtga gtgcgttggt actgacgttg tggggaagcg ataaaatcgt tgctaccggc
     3061 gtggcgatta tctgggggct aacttttgca ttggttcccg tcggctggtc aacgtggatc
     3121 acccgctcgc tggccgatca ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag
     3181 cttgctaata cctgtggcgc ggcaatcggc ggttatgcgc tggataatat tggtctgact
     3241 tcgccgttga tgttgtccgg cacattgatg ttgctgactg cattgttggt tactgcaaag
     3301 gtgaaaatga agaaatcctg atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt
     3361 cttacatatc cgggcattct accttaccca acgcttccca ataattgctc tgattattac
     3421 gttccatcgc cacgatggcc tcgcgtatat gttgtttatt gttttcatct gacaacatta
     3481 acttttccca cgcttcatct gataatgacg catgtggcgt gcaaatttta cgtaaatctt
     3541 gtaataccgt ggttttctct tttgcggact gcagtgagct aaaaggcgac tcggcgtata
     3601 cgatgcctgg catagcgaaa aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt
     3661 caacctttta caaatgtgta tcgaatgaag cataagtgta gtttgctttc atcgctctca
     3721 tcatgctgta cgaaacgcgc ttgcgtggtc agtaagaagt gccagacttt atattccact
     3781 tttattcctt tttattctta tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg
     3841 c
//
LOCUS       DAZPEU010000274         3807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPEU010000274 DAZPEU010000000
VERSION     DAZPEU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.276"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            437..2090
                     /gene="ushA"
                     /locus_tag="ACHU2L_003953"
                     /pseudo
     CDS             437..2090
                     /gene="ushA"
                     /locus_tag="ACHU2L_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
     gene            complement(2101..2271)
                     /locus_tag="ACHU2L_003954"
                     /pseudo
     CDS             complement(2101..2271)
                     /locus_tag="ACHU2L_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2404..2523)
                     /locus_tag="ACHU2L_003955"
                     /pseudo
     CDS             complement(2404..2523)
                     /locus_tag="ACHU2L_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2597..3596)
                     /locus_tag="ACHU2L_003956"
                     /pseudo
     CDS             complement(2597..3596)
                     /locus_tag="ACHU2L_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1053 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tcataattgg ttatgatgca atggccgtcg
      781 gtaatcatga atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat
      841 ttcctttcct ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat
      901 ggaaaatttt catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata
      961 ctgcgaaaac aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg
     1021 aggcgaggag tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg
     1081 caacacatat gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg
     1141 aaatggctcg cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag
     1201 ctcctgtctg catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct
     1261 gtgtaccaga taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg
     1321 tcggtcaggc tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac
     1381 acccagttaa tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt
     1441 acacccccga aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata
     1501 aaggcaaagc acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca
     1561 gaagtaaagt tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag
     1621 aacgtattga cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga
     1681 gtggtaacat cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa
     1741 gtattgattt gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac
     1801 cggattccgg agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa
     1861 tcgaaatgct aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa
     1921 catttagctt taatgctact ggtggggatg gctatccacg tattgataat agacccggat
     1981 acataaatac cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac
     2041 cgctggatgc tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt
     2101 gacgatcatc acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg
     2161 tatgtttgcg atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt
     2221 gctgaattta accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt
     2281 cgctgattaa agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat
     2341 tgatatttac taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc
     2401 cggtcagtcc tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt
     2461 cagacggcat gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga
     2521 accccgccgg agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc
     2581 gtcatacggc tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct
     2641 tcggatactg tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt
     2701 atttagactg catccactta agcaaacgtg actgcattgc actccacagt cgatagctga
     2761 agtttctgga ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc
     2821 gcctcgcaaa atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg
     2881 tttctgttat tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt
     2941 cgcctttgaa attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca
     3001 tcatcggcgt acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag
     3061 gcatagtgca ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct
     3121 cgaccccgtg ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca
     3181 atgtacatca ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca
     3241 tgatcgatat catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag
     3301 cagcgtttcc tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac
     3361 gagtctgcat ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg
     3421 gcgatccggt ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga
     3481 atgtccacac gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt
     3541 ttgtagagat tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct
     3601 gccgccccac ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca
     3661 ggaaatggct ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc
     3721 gccgcaccat cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc
     3781 gctactacgg tttactccgt ccctgta
//
LOCUS       DAZPEU010000275         3801 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPEU010000275 DAZPEU010000000
VERSION     DAZPEU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.277"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            140..2419
                     /gene="ydeP"
                     /locus_tag="ACHU2L_003957"
     CDS             140..2419
                     /gene="ydeP"
                     /locus_tag="ACHU2L_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR1358573.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2667..2870
                     /gene="safA"
                     /locus_tag="ACHU2L_003958"
     CDS             2667..2870
                     /gene="safA"
                     /locus_tag="ACHU2L_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR1358574.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            2938..3699
                     /gene="ydeO"
                     /locus_tag="ACHU2L_003959"
     CDS             2938..3699
                     /gene="ydeO"
                     /locus_tag="ACHU2L_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR1358575.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1063 a          831 c          879 g         1028 t
ORIGIN      
        1 tttaacgata atagattaaa aatagaaaga gagtagtccc ttactcttat atatccatgt
       61 tggcgataat ccctttgtat gtactgtgca tcatcgctat tacaaatcct aataattcat
      121 ttccacacag gataagtaga tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg
      181 ttggggtgct gttaaatccg tagcgaatgc agtacgtaag cagatggata tacgccatga
      241 tgttattgcc atgtttgaca tgaataagcc agagggcttt gactgtccgg gttgtgcatg
      301 gccagatcct aagaacagtg cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc
      361 ttgggaagtc acggataagc aggtaaatgc ctctttcttt gctgagaata cggttcaatc
      421 attacttacc tggggagatc acgagcttga ggctgcgggg cgtctcactc agcctttgaa
      481 atatgatgcc gtcagcgact gttacaagcc attaagctgg caacaagctt tcgacgaaat
      541 tggcgcacgc cttcaaagct atagtgatcc caatcaggtt gaattctata cttcgggccg
      601 cacttccaat gaagctgcct ttctttatca gctttttgcc cgtgaatacg ggagcaataa
      661 ctttcccgac tgctccaaca tgtgccatga accgacaagc gtgggtttgg cagcgagtat
      721 cggtgtaggt aaagggaccg tgttgctgga agactttgag aagtgcgatt tagtcatttg
      781 cattgggcat aaccctggta caaaccaccc tcgcatgctg acttcgttgc gcgctttagt
      841 gaaacgggga gcgaaaatga tcgccatcaa tcctctacag gaacgtggcc tggagcgatt
      901 taccgcaccg caaaacccgt ttgaaatgct gacgaactct gagactcagt tggccagtgc
      961 ctactacaac gtgcgcattg gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt
     1021 aattgagcgc gatgatgctg caagtgccgc aggtcggccc tcattgcttg atgacgaatt
     1081 tattcaaacg cataccgtcg gctttgacga gctacgccgt gacgttctca attccgagtg
     1141 gaaagatatc gaacgtattt ctggactaag tcagacacaa atcgccgaac tggctgacgc
     1201 atatgccgct gccgaacgca ccattatctg ttacggaatg gggatcactc agcacgaaca
     1261 tggtacccag aacgtgcagc aactggtcaa tttgctgttg atgaaaggta acattggcaa
     1321 gcctggtgcg ggtatctgcc cactacgtgg tcactctaat gtacagggcg accgaaccgt
     1381 cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc ctgggtgagc gctatggctt
     1441 ccccccacct catgcacctg gacatgctgc aattgccagc atgcaagcaa tatgtacggg
     1501 gcaggctcga gcattgatct gcatgggggg caactttgca ctggcaatgc cagatcggga
     1561 agcgagcgct gtaccgttaa cgcaattaga tttggcggta cacgtagcca ctaagcttaa
     1621 ccgctctcat ctgctgaccg cacggcatag ctatattctg ccggttctgg ggcgtagcga
     1681 gattgatatg caaaaaagcg gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat
     1741 tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt
     1801 ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg gtagcctggg agtatctggt
     1861 ggaagattat gatcgcattc gcaatgacat tgaagctgtg ctgccagagt tcgccgacta
     1921 taaccagcgc atccgtcatc ccggtggttt tcacctgata aatgcagctg ctgaaaggcg
     1981 ctggatgacg ccgtcaggta aggctaattt cattaccagc aaagggctgt tagaagatcc
     2041 ctcttcagcg tttaacagta agctggtcat ggcgacagta cgcagccacg atcagtacaa
     2101 cacgacgatt tatggtatgg atgatcgcta tcgaggggta ttcggtcaac gagatgtggt
     2161 ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct
     2221 tattgcgctt acgccagacg gtaagcgcag ctcacgccgc atggatagat taaaagtggt
     2281 catttaccct atggctgacc gctcactggt gacctatttt ccagaatcga atcacatgct
     2341 aacacttgat aaccacgatc cattaagtgg tattcctggc tataaaagta ttccggttga
     2401 attagaacca tcaaattaat gcctcttctc atttcttctg ctgtcatccg cacagcagaa
     2461 gaattcctca ttgactatta ttgcgcaatt tgctcacatg gattaaatta aactacatac
     2521 tataagatat aaacttctgc ctacagctgt aagaaactcc gctcagtact gaagcaccag
     2581 tcctatttct tcttttctcc agcctgttat attaagcata ctgattaacg acttttaacg
     2641 ttatccgcta aataaacata tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc
     2701 aaagagataa ctataaagaa atcatgtctg taattgtggt tgtcttatta ctgacactta
     2761 cgttgatagc cattttttcg gcaattgatc agctgagtat ttcagaaatg ggtcgcattg
     2821 caagagatct tacacatttc attatcaata gtttgcaaga ctggaaataa acagcaaatt
     2881 ataaatgtga aatgtttaaa aggtatcgat aaaactttat tgttttaagg agataaaatg
     2941 tcgctcgttt gttctgttat atttattcat catgctttca acgctaacat tttagataaa
     3001 gattacgcct tctccgacgg cgagatcctg atggtagata acgctgttcg tacacatttt
     3061 gaaccttatg agcggcattt taaagaaatc ggatttaatg aaaataccat taaaaaatat
     3121 ctacaatgca ctaacatcca gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct
     3181 tcaaatgtac cgactggatt gcttaatgaa atgattgctt atctcaactc ggaagaacgc
     3241 aatcatcata atttttcaga acttttgctt ttttcttgcc tgtctatttt tgccgcatgc
     3301 aaaggtttca ttacactatt aactaacggt gtgctatccg tttccgggaa agtgagaaat
     3361 attgtcaaca tgaagctggc gcacccatgg aagctgaaag atatttgtga ctgcctgtac
     3421 atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc aaacgacatt ctcacagatt
     3481 ctattagatg ccagaatgca gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat
     3541 aaaattgccg aacaatgtgg ttatgccagt acatcttatt ttatttatgc gttccgcaaa
     3601 catttcggca acagtccgaa gagagtttct aaggagtacc gttgtcaaag gcacacgggt
     3661 atgaatacgg gcaacacgat gagtgcttta gctatttgat tatttgctaa cgagtagtca
     3721 accacgcacg ctacgtaaga attatatggg gcagccattc cctgccccgc gttgttttta
     3781 ggcgatatat ttattgaaat a
//
LOCUS       DAZPEU010000276         3737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPEU010000276 DAZPEU010000000
VERSION     DAZPEU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.278"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            145..591
                     /locus_tag="ACHU2L_003960"
     CDS             145..591
                     /locus_tag="ACHU2L_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR1358576.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            602..2053
                     /locus_tag="ACHU2L_003961"
     CDS             602..2053
                     /locus_tag="ACHU2L_003961"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR1358577.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2043..3113
                     /locus_tag="ACHU2L_003962"
     CDS             2043..3113
                     /locus_tag="ACHU2L_003962"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1358578.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            3113..>3737
                     /locus_tag="ACHU2L_003963"
     CDS             3113..>3737
                     /locus_tag="ACHU2L_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR1358579.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT          839 a          975 c         1010 g          913 t
ORIGIN      
        1 ttaaataacc attatgtgca ttactccgca aatctgacct ttcactctgt ctcaaattgt
       61 cattgtttac cgacatatcg cctctcatca cttaatcgct ccaccattac aattgattaa
      121 aatatatttt atggagtccg attaatggca gctcttactg caagctgtat tgacctgaat
      181 attcagggca atggcgctta ttccgttctg aagcagttgg cgacaatagc gttacaaaac
      241 ggttttatca ccgactcaca ccagttcctg caaaccctgc tgctgcgcga aaaaatgcac
      301 tctactggat ttggttccgg tgtcgcagtg ccgcacggta aaagcgcctg cgttaaacaa
      361 ccgttcgtat tattcgcccg caaagcgcag gctattgact ggaaagccag cgatggcgaa
      421 gacgtcaatt gctggatctg cctcggcgtg ccgcaaagcg gcgaagagga tcaggtcaaa
      481 atcatcggca cactgtgtcg caaaattatt caccaggaat ttattcatca actgcaacag
      541 ggcgataccg accaggtgct ggccttgtta aatcaaaccc tcagctcata aggaagtggc
      601 gatggagtca tccttacgta ttgtcgcgat caccaactgc cccgccggga tcgctcacac
      661 ctacatggtg gcggaagccc tggaacagaa agcccgttct ctcggccata ccataaaagt
      721 ggaaactcaa gggtccagtg gcgttgaaaa ccgcttatcc agcgaagaga ttgccgctgc
      781 cgattacgtc attcttgcta ccgggcgtgg cctgagcggt gatgatcgcg cgcggtttgc
      841 cgggaagaaa gtttatgaga ttgccatctc cctggcgttg aaaaatatcg accagatttt
      901 cagcgaatta ccgacaaact cgcagctttt tgccgcagat agcggcgtga agcttggcaa
      961 acaggaagtg caaagcggca gcgtgatgag ccacctgatg gcgggcgtct ctgcggcgct
     1021 gccgtttgtc atcggcggtg gtatcctggt ggcgcttgcc aacatgctgg tgcagttcgg
     1081 tttgccgtat accgatatgt cgaaaggcgc gccgtcgttt acctgggtag ttgaatccat
     1141 cggttacctt ggcttcacct ttatgatccc catcatgggc gcttatattg cctcgtcgat
     1201 tgccgataaa ccggctttcg ctccggcgtt tctggtttgc tatctggcga acgacaaagc
     1261 gctgctcggc acccagtcgg gtgcgggatt cctcggcgcg gtggtgctgg ggctggcgat
     1321 tggctatttc gtcttctggt tccgcaaagt gcgccttggc aaagcattac aaccgctgct
     1381 cggttcaatg ctgatcccgt tcgttactct gctggttttc ggcgtgctca cttactacgt
     1441 tatcgggccg gtgatgtccg acctcatggg cgggctgctc cacttcctga ataccattcc
     1501 tccatcaatg aagtttgcgg cagcgtttct ggtcggtgcg atgctggcgt tcgatatggg
     1561 cggccccatc aataaaaccg cctggttctt ctgcttctca ctgctggaaa aacacattta
     1621 tgactggtac gccatcgtcg gcgttgtcgc gttgatgccg cccgttgcgg cgggtcttgc
     1681 gacgttcatt gcgcccaaac tgtttacccg acaggaaaaa gaggctgcca gtagtgccat
     1741 tgtggtcggt gccacagtcg caaccgaacc ggctatacct tacgctctgg cagctccgtt
     1801 gccgatgatt accgccaata cgctggcggg cggcattacc ggcgtgctgg tgatcgcttt
     1861 cggaatcaaa cgtctggcac ctggtttggg tatctttgac ccgctgatcg gcctgatgtc
     1921 gccggtaggt tcgttttatc tggtgctggc gattggcctc gcgctgaaca tttcattcat
     1981 tatcgtcctg aaaggattgt ggttacgccg caaagcgaaa gccgcgcaac aggagcttgt
     2041 ccatgaacat tgagttactg caacagttgt gcgaagccag cgccgttagc ggcgatgaac
     2101 aggaagttcg cgacatcctg ataaacacgc tggaaccttg cgttaatgaa atcacctttg
     2161 atggtctggg cagctttgtt gcccgtaagg gaaataaagg tccaaaagtt gccgttgtcg
     2221 ggcatatgga tgaagtcggc tttatggtca cccacatcga cgagagcggt tttctgcgtt
     2281 tcaccaccat tggtggctgg tggaatcagt cgatgctcaa ccatcgggta accatacgca
     2341 cacacaaggg agtgaaaatc cctggtgtga ttggtttcgt cgcgcctcat gcgttaacgg
     2401 aaaagcaaag gcaacagccg ctgtcatttg atgagatgtt cattgatatt ggcgcaaaca
     2461 gtcgcgatga ggtggaaaaa cgcggtgtgg cgatgggcga ttttattagc ccggaagcca
     2521 attttgcctg ctggggcgaa gataaagtgg tcggcaaggc gctggataac cgcatcggct
     2581 gcgcaatgat ggctgaacta ttgcagacgg tgaataatcc cgaaattacg ctgtatggcg
     2641 tcggcagcgt ggaagaagaa gttgggctac gcggggcaca aacctcggcg gaacacatta
     2701 aaccggatgt agtgatcgtg ctggataccg ctgtcgcagg cgatgttccg ggcattgata
     2761 acattaaata cccgctgaaa ctgggccagg ggccggggct gatgctgttt gacaagcgct
     2821 acttccccaa ccagaaactg gtagcagcgt taaaaaactg tgccgcacat aacgatttac
     2881 cgctgcaatt ttccaccatg aaaaccggag cgacggatgg cgggcgctac aacgtgatgg
     2941 gcggcgggcg tccggttgtt gcgctgtgcc tgccaacccg ctatctgcac gctaacagcg
     3001 gcatgatttc aaaagccgat tacgatgctc tgctcacgtt gatacggggt tttctgacga
     3061 ccttaaccgc ggagaaagtc aacgcgttta gccagttccg tcaggtggat taatgctaaa
     3121 cgaacgccag ttaaagattg tcgatctgct ggagcaacag ccgcgcacgc ctggtgagct
     3181 ggcgcaacag actggcgttt caggcaggac catcctgcgt gatattgact atctcaactt
     3241 tacccttaac ggcaaagccc gcattttcgc cagtggcagt gcgggctatc agctggaaat
     3301 cttcgagcgc cgcagctttt ttcagttgct gcaaaagcac gataacgacg atcggctgct
     3361 ggcgctgtta ttactgaata ctttcactcc ccgtgcgcaa ctcgcctcgg cgcttaattt
     3421 gccagaaacg tgggtagcag agcgtctgcc ccagttaaaa cagcgttatg aacgcacttg
     3481 ttgcctggcc agccgccctg gtttgggcca tttcattgat gagacagaag agaaactcgt
     3541 tatcttgctg gcgaacttgc tgcgcaaaga tccgttttta attccgctgg cgggcataac
     3601 acgagacaac cttcagcatt tatccacggc ctgcgacaac caacaccgct ggccgctcat
     3661 acagggtgat tatctctcca gcctgattct ggcgatttac gccctgcgta atcaactgac
     3721 cgatgagtgg ccgcaat
//
LOCUS       DAZPEU010000277         3728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPEU010000277 DAZPEU010000000
VERSION     DAZPEU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.279"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..224
                     /locus_tag="ACHU2L_003964"
     CDS             <1..224
                     /locus_tag="ACHU2L_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1358580.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACHU2L_003965"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACHU2L_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1358581.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACHU2L_003966"
     CDS             518..1108
                     /locus_tag="ACHU2L_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1358582.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACHU2L_003967"
     CDS             1348..1608
                     /locus_tag="ACHU2L_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR1358583.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACHU2L_003968"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACHU2L_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR1358584.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="ACHU2L_003969"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="ACHU2L_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR1358585.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="ACHU2L_003970"
     CDS             3457..3648
                     /locus_tag="ACHU2L_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358586.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1020 a          907 c          934 g          867 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaaca
//
LOCUS       DAZPEU010000278         3667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPEU010000278 DAZPEU010000000
VERSION     DAZPEU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.280"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(38..82)
                     /locus_tag="ACHU2L_003971"
     CDS             complement(38..82)
                     /locus_tag="ACHU2L_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358587.1"
                     /translation="MKRNDKILHYEGLN"
     gene            201..1925
                     /gene="ilvI"
                     /locus_tag="ACHU2L_003972"
     CDS             201..1925
                     /gene="ilvI"
                     /locus_tag="ACHU2L_003972"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR1358588.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1928..2419
                     /gene="ilvN"
                     /locus_tag="ACHU2L_003973"
     CDS             1928..2419
                     /gene="ilvN"
                     /locus_tag="ACHU2L_003973"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1358589.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2431..2517
                     /locus_tag="ACHU2L_003974"
     CDS             2431..2517
                     /locus_tag="ACHU2L_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR1358590.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2599..3603
                     /gene="cra"
                     /locus_tag="ACHU2L_003975"
     CDS             2599..3603
                     /gene="cra"
                     /locus_tag="ACHU2L_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR1358591.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          852 a          879 c         1017 g          919 t
ORIGIN      
        1 aaattgctgt aagttgtggg attcagccga tttattatca atttaatcct tcgtaatgga
       61 ggattttatc gtttcttttc acctttcctc ctgtttattc ttattaccct gcgtttatgt
      121 ctctggctgc caattgctta agcaagatcg gacggttaat gtgttttaca cattttttcc
      181 gtcaaacagt gaggcaggcc atggagatgt tgtctggagc cgagatggtc gtccgatcgc
      241 ttatcgatca gggcgttaaa caagtattcg gttatcccgg aggcgcagtc cttgatattt
      301 acgatgcatt gcataccgtg ggtggtattg atcatgtatt agttcgtcat gagcaggcgg
      361 cggtgcatat ggccgatggc ctggcgcgtg cgaccgggga agtcggcgtc gtgctggtaa
      421 cgtcgggtcc aagggcgacc aatgcgatta ccggcatcgc caccgcttat atggattcca
      481 ttccattagt tgtcctttcc gggcaggtag cgacctcgtt gataggttac gatgcctttc
      541 aggagtgcga catggtgggg atttcgcgac cggtggttaa acacagtttt ctggttaagc
      601 aaacggaaga cattccgcag gtgctgaaaa aggctttctg gctggcggca agtggtcgcc
      661 caggaccagt ggtcgttgat ttaccgaaag atattcttaa tccggcgaac aaattaccct
      721 atgtctgccc tgagtcggtc agtatgcgtt cttacaatcc cactactacc ggacataaag
      781 ggcaaattaa gcgtgctctg caaacgctgg tagcggcaaa aaaaccggtt gtctacgtag
      841 gcggtggggc aatcatggcg ggctgccatc agcagttgaa agaaacggtg gaggcgttga
      901 atctgcccgt tgtttcctca ttgatgggaa tgggggcgtt tccggcaacg catcgtcagg
      961 cactgggcat gctgggaatg cacggtacct acgaagccaa tatgacgatg cataacgcgg
     1021 atgtgatttt cgccgtcggg gtacgatttg atgaccgaac gacgaacaat ctggcaaagt
     1081 actgcccaaa tgccactgtt ctgcatatcg atattgatcc tacttccatt tctaaaaccg
     1141 tgactgcgga tatcccgatt gtgggggatg ctcgccaggt cctcgaacaa atgcttgaac
     1201 tcctgtcgca agaatccgcc catcaaccgc tggatgagat ccgcgactgg tggcagcaga
     1261 ttgaacagtg gcgcgctctt cagtgcctga aatatgacac tcacagtgaa aagattaaac
     1321 cgcaggcggt gatcgagact ctttggcggt tgactaatgg ggacgcttac gtgacgtccg
     1381 atgtcgggca gcaccagatg tttgctgcgc tttattatcc attcgacaaa ccgcgtcgct
     1441 ggatcaattc cggcggtctc ggcacgatgg gttttggttt acctgcggca ctgggcgtca
     1501 aaatggcgtt gccagaagaa accgtggttt gcgtcactgg cgacggcagt attcagatga
     1561 acatccagga actgtctacc gcgttgcaat acgagttgcc cgtactggtg gtgaatctca
     1621 ataaccgcta tctggggatg gtgaaacagt ggcaggacat gatctattcc ggccgtcatt
     1681 cacaatctta tatgcaatca ctacccgatt tcgtccgtct ggcggaagcc tatggtcacg
     1741 tcgggatcca gatctctcat ccgcatgagc tggaaagcaa acttagcgag gcgctggaac
     1801 aggtgcgcaa taatcgcctg gtgtttgttg atgttaccgt cgatggcagt gagcacgtct
     1861 acccgatgca gattcgcggg ggcggaatgg atgaaatgtg gttaagcaaa acggagagaa
     1921 cctgattatg cgccggatat tatcagtctt actcgaaaat gaatcaggcg cgttatcccg
     1981 cgtgattggc cttttttccc agcgaggcta caacattgaa agcctgaccg ttgcgccaac
     2041 cgacgatccg acattatcgc gtatgaccat ccagaccgtg ggcgatgaaa aagtacttga
     2101 gcagatcgaa aagcaattac acaagctggt cgatgtcttg cgcgtgagtg agttggggca
     2161 gggcgcgcat gttgagcggg aaatcatgct ggtgaaaatt caggccagcg gttacgggcg
     2221 tgacgaagtg aaacgtaata cggaaatatt ccgtgggcaa attatcgatg tcaccccctc
     2281 gctttatacc gttcaattag caggcaccag cgataagctt gatgcatttt tagcatcgat
     2341 tcgcgatgtg gcgaaaattg tggaagttgc tcgctctggt gtggtcggac tttcgcgcgg
     2401 cgataaaata atgcgttgag aatgatctca gtgcgcaatt tgcagcccaa catgtcacgt
     2461 tgggcttttt ttgcgaaatc agtgggaacc tggaataaaa gcagttgccg cagttaattt
     2521 tctgcgctta gatgttaatg aatttaaccc ataccagtac aatggctatg gtttttacat
     2581 tttacgcaag gggcaattgt gaaactggat gaaatcgctc ggctggcggg agtgtcgcgg
     2641 accactgcaa gctatgttat taacggcaaa gcgaagcaat accgtgtgag cgacaaaacc
     2701 gttgaaaaag tcatggctgt ggtgcgtgag cacaattacc acccgaacgc cgtggcagct
     2761 gggcttcgtg ctggacgcac acgttctatt ggtcttgtga tccccgatct ggagaacacc
     2821 agctataccc gcatcgctaa ctatcttgaa cgccaggcgc ggcaacgggg ttatcaactg
     2881 ctgattgcct gctcagaaga tcagccagac aacgaaatgc gctgcattga gcacctttta
     2941 cagcgtcagg ttgatgccat tattgtttcg acgtcgttgc cacctgagca tcctttttat
     3001 caacgctggg ctaacgaccc gttcccgatt gtcgcgctgg accgcgccct cgatcgtgaa
     3061 catttcacca gcgtggttgg tgccgatcag gatgatgccg aaatgctggc ggaagagtta
     3121 cgtaagtttc ccgccgagac ggtgctttat cttggcgcgc tgccggagct ttctgtcagc
     3181 ttcctgcgtg aacaaggttt ccgtactgct tggaaagatg atccgcgcga agtgcatttc
     3241 ctgtatgcca acagctatga gcgggaggcg gctgcccagt tattcgaaaa atggctggaa
     3301 acgcatccga tgccgcaggc gctgttcaca acgtcgtttg cgttgttgca aggagtgatg
     3361 gatgtcacgc tgcgtcgcga cggcaaactg ccttctgacc tggcaattgc cacctttggc
     3421 gataacgaac tgctcgactt cttacagtgt ccggtgctgg cagtggctca acgtcaccgc
     3481 gatgtcgcag agcgtgtgct ggagattgtc ctggcaagcc tggacgaacc gcgtaagcca
     3541 aaacctggtt taacgcgcat taaacgtaat ctctatcgcc gcggcgtgct cagccgtagc
     3601 taatccgcga acaaaaatac gcgccaggtg aatttccctc tggcgcgtag agtacgggac
     3661 tggacat
//
LOCUS       DAZPEU010000279         3655 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPEU010000279 DAZPEU010000000
VERSION     DAZPEU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.281"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            125..1276
                     /gene="yiaY"
                     /locus_tag="ACHU2L_003976"
     CDS             125..1276
                     /gene="yiaY"
                     /locus_tag="ACHU2L_003976"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR1358592.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1441..2979
                     /gene="aldB"
                     /locus_tag="ACHU2L_003977"
     CDS             1441..2979
                     /gene="aldB"
                     /locus_tag="ACHU2L_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR1358593.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3198..3341)
                     /locus_tag="ACHU2L_003978"
     CDS             complement(3198..3341)
                     /locus_tag="ACHU2L_003978"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358594.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3524..>3655
                     /locus_tag="ACHU2L_003979"
     CDS             3524..>3655
                     /locus_tag="ACHU2L_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1358595.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          917 a          862 c          949 g          927 t
ORIGIN      
        1 ttgtttaatg aatacaatgt gctttttatt agattaattt tgataattaa taataatgat
       61 catgttttcg aatgaaaatc ctcagtaagc tgcccaccct tttttacact ttcaggagtg
      121 tgttatggca tcttcaactt tctttattcc ttctgtgaat gtcatcggcg ctgattcatt
      181 gactgatgca atgaatatga tggcagatta tggatttacc cgtaccttaa ttgtcactga
      241 caatatgtta acgaaattag gtatggcggg tgatgtgcaa aaagcactgg aagaacgcaa
      301 tatttttagc gttatttatg atggcaccca acctaacccc accacggaaa acgtcgccgc
      361 aggtttgaaa ttacttaaag aaaataattg cgatagcgtg atctccttag gcggtggttc
      421 tccacacgac tgcgcaaaag gtattgcgct ggtggcagcc aatggcggcg atattcgcga
      481 ttacgaaggc gttgaccgct ctgcaaaacc gcagctgccg atgatcgcca tcaataccac
      541 ggcgggtacg gcctctgaaa tgacccgttt ctgcatcatc actgacgaag cgcgtcataa
      601 caaaatggcg attgttgata agcacgtgac tccgctgctt tctgtcaatg actcctctct
      661 gatgattggt atgccgaagt cactgaccgc cgcaacgggt atggatgcct taacgcacgc
      721 tatcgaagcg tatgtttcta ttgccgccac gccgatcact gacgcttgtg cactgaaagc
      781 cgtgaccatg attgccgaaa acctgccgtt agccgttgaa gatggcagta atgcgaaagc
      841 gcgtgaagca atggcttatg cccagttcct tgccggtatg gcgttcaata atgcttctct
      901 gggttatgtt catgcgatgg cgcaccagct gggcggtttc tacaacctgc cacacggtgt
      961 atgtaacgcc gttctgctgc cgcatgttca ggtattcaac agcaaagtcg ccgctgcacg
     1021 tctgcgtgac tgtgccgctg caatgggcgt gaacgtgaca ggtaaaaacg acgcggaagg
     1081 tgctgaagcc tgcattaacg ccatccgtga actggcgaag aaagtggata tcccggcagg
     1141 cctacgcgac ctgaacgtga aagaagaaga tttcgcggtt ctggcgacta atgccctgaa
     1201 agatgcctgt ggctttacta acccgatcca ggcaactcac gaagaaattg tggtgattta
     1261 tcgcgccgca atgtaatcat cattccaaca acggctggca aattgtcagc cgctttttca
     1321 actatctctg taatccttgc ccgtaaattc gtgatagctg tcgtaaagct gttaccgact
     1381 ggcgaagatt tcgccagtca cgtctaccct tgttatacct cacaccgcaa ggagacgatc
     1441 atgaccaata atcccccttc agcacagatt aagcccggcg agtatggttt ccccatcaag
     1501 ttaaaagccc gctacgacaa ctttattggc ggtgaatggg tagcccctgc cgatggtgag
     1561 tattaccaga atctgacgcc ggtgaccggg cagctgctgt gcgaagtggc gtcttcgggc
     1621 aaacgagaca tcgatctggc gctggatgct gcgcacaaag tgaaagataa atgggcgcac
     1681 acctcggtgc aggatcgcgc ggcgattctg tttaagattg ccgatcgaat ggaacaaaac
     1741 ctcgagctgt tagcgacagc tgaaacctgg gataacggca aacccattcg cgaaaccagt
     1801 gctgcggatg taccgctggc gattgaccat ttccgctatt tcgcctcgtg tattcgggcg
     1861 caggaaggtg ggatcagtga agttgatagc gaaaccgtgg cctatcattt ccatgaaccg
     1921 ttaggcgtgg tggggcagat tatcccgtgg aacttcccgc tgctgatggc gagctggaaa
     1981 atggctcccg cgctggcggc gggcaactgt gtggtgctga aacccgcacg tcttaccccg
     2041 ctttctgtac tgctgctaat ggaaattgtc ggtgatttac tgccgccggg cgtggtgaac
     2101 gtggtcaatg gcgcaggtgg ggaaattggc gaatatctgg cgacctcgaa acgcatcgcc
     2161 aaagtggcgt ttaccggctc aacggaagtg ggccaacaaa ttatgcaata cgcaacgcaa
     2221 aacattattc cggtgacgct ggagttgggc ggtaagtcgc caaatatctt ctttgctgat
     2281 gtgatggatg aagaagatgc ctttttcgat aaagcgctgg aaggctttgc actgtttgcc
     2341 tttaaccagg gcgaagtttg cacctgtccg agtcgtgcct tagtgcagga gtcaatctac
     2401 gaacgcttta tggaacgcgc catccgccgt gtcgaaagca ttcgcagcgg taacccgctc
     2461 gacagcgtga cgcaaatggg cgcgcaggtt tctcacgggc aactggaaac catcctcaac
     2521 tacattgata tcggtaaaaa agagggcgct gacgtgctca caggcgggcg gcgcaagctg
     2581 ctggaaggtg aactgaaaga cggctactac ctcgaaccga cgattctgtt tggtcagaac
     2641 aatatgcggg tgttccagga ggagattttt ggcccggtgc tggcggtgac caccttcaaa
     2701 acgatggaag aagcgctgga gctggcgaac gatacgcaat atggcctggg cgcgggcgtc
     2761 tggagccgca acggtaatct ggcctataag atggggcgcg gcatacaggc tgggcgcgtg
     2821 tggaccaact gttatcacgc ttacccggca catgctgcgt ttggtggcta caaacaatca
     2881 ggtatcggtc gcgaaaccca caagatgatg ctggagcatt accagcaaac caagtgcctg
     2941 ctggtgagct actcggataa accgttgggg ctgttctgat ataagaagct ggtcgcattg
     3001 ggtattcatt gcctgatgcg acgcttacgc gtcttatcat gcctacggga acctgaccgt
     3061 aggccggata aggcgtacac gccgcatccg gcataaacaa agcgcacgtt gttacaattt
     3121 acgttttaaa cctacgtgcg caataaccca gactacgcca acagaagtgc gtttctgggg
     3181 cgtgttttgt caggagttta atctcgcgtt tcgatggtga gcgggcgaac caggccgtaa
     3241 atcaccattg gtgatccgag gccaatcagc aataaacggt agataattga aggtttaata
     3301 cgcttaatct gtactgtgga tgttttactg gagttattca ttagtttggc caatagttcc
     3361 actatttcaa tatgataaac ctcgtcctgt tgaaaaacca gacagcacat ttcagaaagt
     3421 atataaaaaa caatatgtcg ttatataaat aaggaaattg cactgcatca tggcatttca
     3481 taatatgcgt taattgttct tttttatatt ctttgaggga aatatgttcc tggactattt
     3541 tgcactggga gtgcttattt ttgtatttct ggtgatattc tatgggatta ttattttaca
     3601 tgatattccc tacctgattg ctaaaaaacg taatcatcct catgccgacg ccatt
//
LOCUS       DAZPEU010000280         3633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPEU010000280 DAZPEU010000000
VERSION     DAZPEU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.282"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..481
                     /locus_tag="ACHU2L_003980"
     CDS             <1..481
                     /locus_tag="ACHU2L_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR1358596.1"
                     /translation="DLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMMAEWLGGD
                     VSPGINVGYIFADADVAPEPGDEHGLSNNAINAVIFNLACRIAPDYALEAPAKLITTA
                     RYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEPCDNGGE"
     gene            482..1900
                     /locus_tag="ACHU2L_003981"
     CDS             482..1900
                     /locus_tag="ACHU2L_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR1358597.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGCEQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            1900..2748
                     /locus_tag="ACHU2L_003982"
     CDS             1900..2748
                     /locus_tag="ACHU2L_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR1358598.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            2748..3203
                     /locus_tag="ACHU2L_003983"
     CDS             2748..3203
                     /locus_tag="ACHU2L_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR1358599.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            3206..>3633
                     /locus_tag="ACHU2L_003984"
     CDS             3206..>3633
                     /locus_tag="ACHU2L_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR1358600.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY"
BASE COUNT          941 a          896 c          944 g          852 t
ORIGIN      
        1 tgatttagtt cttgcggcat tacgtaagct cggtgtggca tcaaatgcca cgttaaccga
       61 tgtagaaccg cagtctatgg aagacggcgt caacgacctt gaaatgatga tggctgaatg
      121 gcttggcggt gatgtgtcac ctggtatcaa cgttggctac attttcgctg atgcagatgt
      181 cgctccagaa ccgggcgatg agcacggttt atcaaataac gctatcaatg ccgtcatttt
      241 caaccttgcc tgccgcattg ctccagatta tgcgctggaa gcgccagcaa aacttataac
      301 cactgccaga tacgggaaag agcgactcgt caaactgtct gcaatggaca gagcaaaagc
      361 cgctaaatgt aagtccggtt atccaaaccg tatgcctgtt ggtagcggta accagttggc
      421 gaagtggaat ggttggaatt acttccaccg gaaggaacct tgcgataacg ggggcgaata
      481 aatgccgatt cagcaacttc cgcttatgaa aggtgtcggc aaagatttcc gaaacgccga
      541 ctacatcgac tatctgccag tgaatatgct ggctacaccc aaagaaatac tcaacagcag
      601 cggatatctt cgctcattcc cgggcattgc caaacgttct gatgtgaatg gtgtatcgcg
      661 cggtgtcgag tacaacatgg cgcagaatgc tgtttatcgc gtttgcggtg gcaagttgta
      721 taagggagaa agcgaggtcg gtgatgttgc cggaagtggt cgcgtatcaa tggcgcatgg
      781 tcggacatca caggcggtag gcgttaatgg tcaactggtc gagtatcgct atgatggcac
      841 ggttaaaacc gtctcaaact ggcctacaga cagtggattc actcagtatg agttaggttc
      901 agttcgcgac attacgcgct tacgtgggcg ttatgcgtgg tcaaaagacg gcactgattc
      961 atggtttatc actgaccttg aagatgaatc gcatcctgac cgctacagtg cacaatatcg
     1021 tgccgagtct cagccggacg gcatcatcgg cataggtaca tggcgagact tcatcgtctg
     1081 ctttggctca tcgacgattg aatatttttc cctgacaggt gcaaccaccg ttggtgccgc
     1141 tttgtatgtc gcacagccat cgctgatggt gcagaaaggt attgccggga cttactgcaa
     1201 aacaccattc gctgattctt atgcgttcat cagcaatccg gcaacaggtg cgccgtctgt
     1261 atacatcatc ggctccggtc aggtgtcacc aatcgccagc gcgagcattg agaaaatcct
     1321 ccgctcctac actgctgatg aactggctga tggcgtgatg gaatcgctgc ggtttgatgc
     1381 gcatgagttg ctgattatcc accttccgcg ccatgtactg gtgtacgacg catcttcaag
     1441 cgccaatggt ccgcaatggt gtgtgctgaa aacaggcctg tatgacgatg tgtaccgcgc
     1501 tatcgacttc atttacgaag gcaatcaaat aacgtgcggc gataagctgg agtccgtgac
     1561 cgggaaattg caattcgaca tcagcagcca gtacgacaag caacaggaac acctgctgtt
     1621 tactccactg ttcaaagcgg ataacgccag agtgttcgac cttgaggttg aatcgtcaac
     1681 tggcgttgcg cagtatgctg accgcctttt tctctctgca accactgacg gcatcaatta
     1741 cgggtgtgag cagatgattg agcagaatga accgttcgtt tacgacaaac gcgttttgtg
     1801 gaagcgagta gggcgcatca ggaaaaatgt cggcttcaaa ttgcgcgtta tcacgaagtc
     1861 acctgtcact ctgtctggct gccagataag gattgagtaa tggcggattc gaatctcaat
     1921 gtgccggtaa tcattcaggc tacacggctc gacacatcag tccttccacg caatatcttc
     1981 tcgcagtcgt atctgcttta cgttatcgca cagggcactg atgttggtaa cgtggccaac
     2041 aaggccaacg aggccggaca gggcgcttat gacgcacaag tcaggaacga tgagcaggat
     2101 gtgattctcg ctgaccatga gcagcgaatt tctgctgcgg aagcaacgct tgttaatcat
     2161 gaggagcgaa tcagccaggc agaatcaact cttcaggaac atgaaacgcg aatcgctcag
     2221 aatgaaagcg atattgcgtc gcttgatacc agagttcagt cgctggaatc gcaggtttca
     2281 gaccatgaaa cgcgcatcga tgctctggag tatgccacta ctcgcaagaa gtcagaggtt
     2341 gtttactctg gcgtatctgt aaccatcccg acagcgccga ccaaccttgt tagcctactg
     2401 aaaacgctca cgccgtcatc cggcacgttg gcaccattct tcgacaccgt taacaacaag
     2461 atggttgtgt tcaacgagaa caaaaccttg ttcttcaagc tgtcgatcgt cgggacgtgg
     2521 cccagcggaa ccgccaacag gtcaatgcag ctaacctttt ccggctctgt tcctgatacg
     2581 ctggtcagca gtcgtaatgc ggcgacaaca accgacaaca tcctgttagc tacattcttc
     2641 agcgtggata aagacggatt tcttgccaca aatggcagta cgttaaccat tcagtcgaat
     2701 ggtgcggcgt ttactgccac aaccatcaag ataatcgcgg agcagtgatg attcagttca
     2761 aaccaacgcg aaacatcgac ctgatagaag ccgtgggaaa tcaccccgac attatcgccg
     2821 ggagcaacaa cggtgatgga tacgactaca agcctgaatg ccgttacttc gaggtgaacg
     2881 tgcacgggca gttcggcggc attgtttact atcaggagat tcagcctttg acctttgatt
     2941 gccacgccat gtacctgcca gaggttcgtg gattcagcaa ggaaatcggg ctggcgttct
     3001 ggcgatacat tctgactaac accaccgttc agtgcgtcac atcgttcgct gcgcgcaaat
     3061 tccgccacgg tcagatgtac tgcgcaatga ttggccttaa gcgagtagga accatcaaga
     3121 aatacttcaa aggcgtggat gacgtgacat tttacagtgc tacacgcgaa gaactaatcg
     3181 acttcctgaa tcacgggaga tagccatgtt atatgcattt aagctgggca gaaaactgcg
     3241 cggcgaggaa ccttattgcc ctgaaaaagg cgggaaaggt ggcagctctg ataaaagtgc
     3301 aaagtatgca gcagaagctc agaagtatgc cgcagacctg caaaatcagc agtggcagac
     3361 gatcatgaaa aaccttgctc cgttcacgcc gcttgcggag cagtatgtta accagcttca
     3421 gaacctttcc agtttagaag gacagggtca ggcacttaat cagtattaca actctcagca
     3481 gtataaagac cttgcaggtc aggctcgtta ccagagtctt gctgctgcgg aggctacggg
     3541 aggacttggt tcgacagcca caagcaatca actggctacg attgctccga ctctcggtca
     3601 gtcgtggtta tcaaaccaga tgagcaatta caa
//
LOCUS       DAZPEU010000281         3548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPEU010000281 DAZPEU010000000
VERSION     DAZPEU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.283"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHU2L_003985"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHU2L_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1358601.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACHU2L_003986"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACHU2L_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR1358602.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHU2L_003987"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHU2L_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR1358603.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIKNYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          772 c          819 g          951 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tttataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAZPEU010000282         3548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPEU010000282 DAZPEU010000000
VERSION     DAZPEU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.284"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..283
                     /locus_tag="ACHU2L_003988"
     CDS             <1..283
                     /locus_tag="ACHU2L_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358604.1"
                     /translation="LQKRIITTAGYIFQKSYILQYAPYALRKKCVLDEALDILAEQRK
                     IRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNRL"
     gene            complement(453..1670)
                     /locus_tag="ACHU2L_003989"
     CDS             complement(453..1670)
                     /locus_tag="ACHU2L_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1358605.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(2037..3023)
                     /gene="ycdU"
                     /locus_tag="ACHU2L_003990"
     CDS             complement(2037..3023)
                     /gene="ycdU"
                     /locus_tag="ACHU2L_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFR1358606.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(3020..3548)
                     /locus_tag="ACHU2L_003991"
                     /pseudo
     CDS             complement(3020..3548)
                     /locus_tag="ACHU2L_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         1069 a          867 c          695 g          917 t
ORIGIN      
        1 gcttcagaaa agaattatca caacagcagg ttacatattc cagaaaagtt atattttaca
       61 atatgcccca tatgctctca ggaaaaaatg tgttctggat gaagcacttg acatactggc
      121 ggaacaaagg aaaataagga ttgatgataa cctggttgtt tatataggaa acacgattac
      181 accatccgaa ctggcaaaaa aactaaatat tcctgcattc gatgccggag ttttcatttg
      241 tgatcatcaa aacatactga agtatcaccg aaacagacta tagatactat cggacaaaaa
      301 ataaattatc acaagtgtat tattcgttat aataatgaat aatcgttatc tgagtgcaat
      361 gccaggtttt cattacgaac tggcattgca tttatttttc aaaaaagaaa gcagtaacca
      421 tttggctact ctgagtatgc cagtccatga aactaagcga attccttagt gtttttagca
      481 agttgagaca tcacataatc cgcccatgcc tgcatcatcg gtacacgctg ctccagtaga
      541 tcagtacgat gatatgccgc ctcaacctta tttttcagcg tatgagcgag cgccctttcc
      601 gccagatccc gcgaataccc ctgttcacta caccagtccc tgaatgttga gcgaaaacca
      661 tgtgccgtgg caactcgccc cggaatgtca ctgacggctt tctttttacg cagaaaactt
      721 gtcaacacca tatcggaaag gatctgctgc tttctgggtg aagggaacac cagttcatca
      781 tgcaggccac gtatattttc cagaatgtaa atagcctgcc gggataaagg aacacgatgc
      841 tgtagcctag ctttcattct ttctgcaggt atagtccata cccgcttatg aaaatcaatt
      901 tcagcccagc gcattcccct ggcttcgccc gagcgagttg ctgtaagtat caccattaat
      961 aacagtgcgc gggtaacatt ataaggttca tcactataca cactggtcgc cacaaaaagc
     1021 ggtaactgcc tccagggcat tgcgggttgg tgttcatcac gtcctcttgt ctgctgagga
     1081 agcaaatggt caaccacatc aacaggattt gccacacaaa aaccgtgcgc ccatccccac
     1141 tgcataacaa catgaatgcg ctgtttaacc cggcttgccg tttctgacaa ggttaaccag
     1201 actggacgca gtgtttctgc cacgtccgca gccgtaatcg aatccagcgt ttttgctccc
     1261 agttgaggaa acgcgtaatt ctcaagcgtc gataaccact gccttacatg ctttggattc
     1321 tcccatccag gagacagttc tgcatgtaca cgcctggctg catcggcaaa tgttgggata
     1381 acgactttct cagattcagc ctttttaatc tccagaggat catcacctgc agcaagttgc
     1441 tctcgcatta tccgggcagt acgtgcagct tcagcaatac tgacctctgg gtaagttccc
     1501 aatccagcat tacgtctttt ttgtgtcacc ggacttacat aacgaaaaac ccatttcccc
     1561 cgccccttta ctgaagaagg atgaagggtc agtccggtaa ttcccccatg gggcaatggt
     1621 ttgtcatcag gtttgatatg tcttgctttc gtatccgtca atactgccat acgctaattc
     1681 ccgtcactct ggtatgccat ccagtatgcc attagtccct cgcttcacta agaatacatt
     1741 agataacatc ggacaacaaa aagtgtaata cattgtttat aaagaaatta tataacaccc
     1801 ctggattaag tcagatttat ttcaggcggt ccccctcacc gccatattta aagaagagcg
     1861 cgtacgaaag tacgggcttt tttttcgtat attgtacaca ccgggtgccc ccgcccacgt
     1921 ctgttagggc gaggggaaat tgtgcacatc aactgtctgg ttactcagtc aacaatcaaa
     1981 atgcgtaaga tagtacagaa catatatgcg ttatctggtc acttcctgta acttcctcac
     2041 ttattaaaat gcccccaacg gaaatacatg tcacatgcca tcataaaatc gttatgccag
     2101 aggaatcgac aatggaaaaa atagtaaatt aagcacggta catagcagag taaaaaatag
     2161 gcttcaaacc aaatccgcca ttccacaagc gttgccaata ccgacaccag cgtaatccac
     2221 atcatttcaa taacccgcaa tatgaataac cgaagccaaa gcggcttggc aaaaaaggtc
     2281 gacaacgtat tttcactgga gaaaaacaga tcgatttcct gcaaaaaaaa cctgcccaga
     2341 aaagcgggcc gcttggagaa gcgcagaaaa aacagattaa tcgccaacac tacgccacaa
     2401 agaatgagca atgcaattat cgatttaaac gttaaccact gcccctctgt cacaaaagaa
     2461 tcactgttac gcagcgggct gatagtaagc aggttaacaa ccgttgagat gcacaccgaa
     2521 agcagcacat agcgagtcag acttttatat tttacttttt tcgctactga aatagcgacc
     2581 tgcttgacat aactggtgcg gatcatccga aacagcagaa agatgaccaa cggcccaagt
     2641 aacgaaatca ggaacagcgc atataacgag ggtagaagct ggtatagccc ccaactcatt
     2701 gcactcaaca tgattaacga cagaaatcct gccaccagag gttgtgtaag aaataattct
     2761 attttgtggt tctgcttttt cttaaaccac gaaaaatcga tattcttcgt gacgcattta
     2821 taagcccagt acgccttgag atcaaaaata aaattataca gcaccagggc cagaatccca
     2881 ctaactacac caatatcctc tgatggaaaa gtaaaccctg catttttcca ataaaagcct
     2941 atcagaataa gtccaatagc ataaatgtat atcagatttc gtttatcctg aaagcgaata
     3001 aaagttaaat aatcaggaat catttgaata cctttgcatc actaaatgct ttacgacaat
     3061 gcataactcg ttgctgatcc acgccagcaa aatcatttaa catattcccc aaatagggct
     3121 tattacttat ttcaaggaaa gctaccgggg ttggcatatt cagaatatgt atcgagagat
     3181 tcagtccatt tcaccgtatg agttaagtgt aatgagagat tttctttgat aattgacgga
     3241 gcgacctcgc tttttgctgt tacgttcatc agaacctgat gctctggcga agcgatatcc
     3301 agtccggcaa gataatcgcg cattgcctga acgccgtctt ccatcaaacg cgtgtgccag
     3361 gcaccgctca caccgagttt aaccggttcg tatccagcgg ccatcagcag cgtggtaaat
     3421 tcattcaacg cagcctgcgt tcccccaata acctgctggc gcggcgtgtt atcacagcta
     3481 atatccagcg caatgcctga ttccgtaatc atcgtctgca gttgttcgcg gttgatgcct
     3541 ttaaccgc
//
LOCUS       DAZPEU010000283         3545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPEU010000283 DAZPEU010000000
VERSION     DAZPEU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.285"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            19..204
                     /gene="ymgJ"
                     /locus_tag="ACHU2L_003992"
     CDS             19..204
                     /gene="ymgJ"
                     /locus_tag="ACHU2L_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR1358607.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            165..284
                     /locus_tag="ACHU2L_003993"
     CDS             165..284
                     /locus_tag="ACHU2L_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1358608.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            319..663
                     /locus_tag="ACHU2L_003994"
                     /pseudo
     CDS             319..663
                     /locus_tag="ACHU2L_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1035..1301)
                     /gene="minE"
                     /locus_tag="ACHU2L_003995"
     CDS             complement(1035..1301)
                     /gene="minE"
                     /locus_tag="ACHU2L_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR1358609.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1305..2117)
                     /gene="minD"
                     /locus_tag="ACHU2L_003996"
     CDS             complement(1305..2117)
                     /gene="minD"
                     /locus_tag="ACHU2L_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR1358610.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2141..2836)
                     /gene="minC"
                     /locus_tag="ACHU2L_003997"
     CDS             complement(2141..2836)
                     /gene="minC"
                     /locus_tag="ACHU2L_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR1358611.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3356..>3545
                     /locus_tag="ACHU2L_003998"
     CDS             3356..>3545
                     /locus_tag="ACHU2L_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1358612.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
BASE COUNT          900 a          815 c          835 g          995 t
ORIGIN      
        1 ttattgtgtg atttttatgt gagcagaaga tattcatcag caacgattac attagtcatt
       61 ttatttttcc gacggcctca ttgtctaaag ataagcgtac gacagtatta tctgaaaaga
      121 gtgatttttt atccaactac acttcagcgc actgcgtgta aaaaatgcct ctttcttatg
      181 cgggatatca tcatttcatc atgatgtctt tggtgagcgg tgaacacaat acacctgcgc
      241 tgtctcttca ggatgaatcc cctggtctgg tgcctgcggg ctgatgttgc agcagagctt
      301 aggtcactta gacgctacta tcatttatcc aatggcatgg aatcgaaatc agtcgatacc
      361 cgcagtatat atcgtgaact gggtgcaacg ctgagttaca acatgcgtct ggggaacggt
      421 atggaaattg aaccctgcct gaaggcggct gtgcgcaaag aatttgtcga tgataatcgg
      481 gtgaaagtga atagtgacgg taattttgtc aatgatttgt cgggcagacg tggaatatac
      541 caggcaggta ttaaagcctc attcagcagt acgttaagcg ggcatttcgg ggtggggtat
      601 agccatggtg ccggtgtgga gtccccgtgg aacgcggtgg ctggtgtgaa ctggtcgttc
      661 tgaccatcaa cgattaaact gcgcttcggc gcagttttcg tttacaggat gttgaaagag
      721 aaaattctgg ggcaaaaaaa gcccgccagt tacggcggga aacctcatcc tatgggagaa
      781 caatgaataa tgaaattgcg gggttatcat ctcccagtat atacacacta ataacatggt
      841 tatttactca accaggcata aacattttgt tctgtgcgtg ggaacagcct taaggtgtaa
      901 agggggaggt ggaaatagca atgaggagta tcagcaagaa tactcgccgc tttaccacaa
      961 cgtggatgag agggatgaaa aactcaaggc agaaatgact ctgccttgaa gataaatgcg
     1021 tttttacagc gggcttattt cagctcttct gcttccggta aggtcacgtt cagctcaaga
     1081 atagaaatat cgccatcttt ttgctcaagc tgtacggtta ccatctcagg atcaatttgt
     1141 acgtatttac aaatgacctc aagaatatct ttacgcaact gcggcagata atgcggttct
     1201 gcatcgctgc gacggcgttc agcaacaata atctgcagcc gttcttttgc aatgttggct
     1261 gtgttcttct tccgcgagag aaagaaatcg agtaatgcca taacttatcc tccgaacaag
     1321 cgtttgagga agcctttctt ctcttcttca atgaagcgga aaggacgttc ttctcccaac
     1381 agacgttcta cggtatctgc gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc
     1441 ggttcaccct ggttagaggc gcgcaatact gattgatcct ctgggatcac gccgacgagt
     1501 ttgatgcgca ggatctccag cacatcttcc atgctcagca tgtcacctct gcttacgcgg
     1561 cctgggttat agcgcgttaa cagcaggtgc tctttaatag gctcttcgcc attttctgcg
     1621 cggcgtgatt ttgacgccag aatgcctaaa atacggtcag agtcgcgtac tgaggagact
     1681 tccgggttgg tggtaataat ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt
     1741 tcaatccctg ccggggagtc acaaacgata aattcaaaat ccatcgcttt cagatcatca
     1801 agaactttgg cgaccccttc acgggtgagg gcatctttat cgcgtgtttg cgatgccgga
     1861 agaatataga gattttcagt acgcttatct ttaattaacg cctgatttag cgttgcatcg
     1921 ccctgaatga cgttgacgaa atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg
     1981 agattacgca ggccgatatc aaaatctatc acgacagttt tctttccctt ctgggccaaa
     2041 ccagtggcga tggccgcgct ggaggttgtc ttaccaacac cccctttacc cgaagtaaca
     2101 acaataatgc gtgccataga aattccttgt taaaaaggga tcaatttaac ggttgaacgg
     2161 tcaaagcgtt ttcgactaac tgcagtcgcg ccgctttgcc ataaaattct gctgggattt
     2221 gatcactcag ccagtattca cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa
     2281 atatttgcgt ttcccggtca ccacttgccc ctgccagcgc acgaccgcgc atcatgccat
     2341 agacatgaat gttcccatcg gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa
     2401 tcagatcaca ttgtggagca taaatacgct gaccggaacg caccggggta tctattaaac
     2461 gcgtttttgt gaccggcatt gtattttgcg ctggagcctg cggtgcggga gctggacgtg
     2521 gcgctttttc ttttccttcc gtcaggatag gcagccccat tttttcaatt tcggctttaa
     2581 gttgcgcatc tttgcagcca cttacgccaa taacccgcaa accggttgcc gaaaccgcct
     2641 tatgcatcgc tgaccagttt accgggtctt ccagtgcact gacgttgagt acaacggggg
     2701 catgttttaa aaatgtgggg gcctgagcga ttttgtcttt cagcgcctga tggataacct
     2761 taggttctgc ctcatgcaga tgaaccacag ataaagtgaa gctactgcct ttaagctcga
     2821 ttggagtgtt tgacatcctg gccttactca attagctatt aatcatcgcc agcgcgcgat
     2881 gatgttccga agactataag gcatgttata gtctggatta tattgaggca agtcaccctc
     2941 ccatttattc agagtaaaag tctattctgt gataaatggc gctgattcat agcttaaaaa
     3001 atacccttgt caatcaaccc attgccgtcg tacttttgat tcttcttatt tacgcttcct
     3061 ttttccgcac cctaatcgtg acagcatctc cagacctgtt taaatatcca gtattgaggc
     3121 aagtgtattt tttcaccacg ccaggctttc ttaactttcc attttttgca gcatcattaa
     3181 agaacagata cccgttatct taatatccgc cacagaattg ctctatcata gaaaccgcag
     3241 aagacgtctg atacttctgt caggtcatcg taactcgtcc aggtcagcgg ttttgattac
     3301 ggattgatga cgaaatgagc ataagtgttg tacggttgga ttatcaggat gtgctatgaa
     3361 tatgatgcgt attatttata tcggattgtc aggtgtgggg atgatgttct catcaatggc
     3421 gtctggacat gacgctggcg gacttcaatc tccgacgtgc ggcgttgttt gcgacccgta
     3481 tatttgtgtg aactcagatg gcatttcccc agagttaaca aggaaatatc tcggcgaaaa
     3541 agccg
//
LOCUS       DAZPEU010000284         3506 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPEU010000284 DAZPEU010000000
VERSION     DAZPEU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.286"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..77
                     /locus_tag="ACHU2L_003999"
     CDS             <1..77
                     /locus_tag="ACHU2L_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1358613.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHU2L_004000"
     CDS             435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHU2L_004000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR1358614.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1490..2614
                     /locus_tag="ACHU2L_004001"
     CDS             1490..2614
                     /locus_tag="ACHU2L_004001"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR1358615.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHU2L_004002"
     CDS             2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHU2L_004002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR1358616.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          927 a          762 c          850 g          967 t
ORIGIN      
        1 cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat tatctgaaca
       61 taaaacacta tcagtaagtt ggagtcatta cccgtttttg acttaaatag caatttaccc
      121 gccaattctt gttgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
      181 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
      241 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
      301 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
      361 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
      421 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
      481 atggggatat gttattaact actcccgtca ttagttctct gaaaaaaaat taccctgacg
      541 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
      601 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
      661 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
      721 atcaatggat ggttgctata ctggttcgct tattaaatgc tcgtgtgaaa atttcccagg
      781 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
      841 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
      901 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
      961 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
     1021 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
     1081 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
     1141 cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
     1201 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
     1261 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg cgcaacagat catattttct
     1321 ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
     1381 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
     1441 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
     1501 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
     1561 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
     1621 atgccctgat gtatttgaag tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
     1681 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
     1741 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
     1801 cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta
     1861 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
     1921 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
     1981 tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga
     2041 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
     2101 agacttcacg cgtaaaggtg ccgatcgttc cattgaagca cttgcttcgt taccggattc
     2161 gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc
     2221 actggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt
     2281 ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg aagcggcggg
     2341 aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaactg ccgtttgtgg
     2401 ctatgcgcat tatattgtcg acgctaattg cggcgaggct attgctgagc cattccgcca
     2461 ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcttg
     2521 ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc
     2581 ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat
     2641 ggcgcggcaa agatcctttt gaggaagtta aaaccttgca gggtgaggta tttcgtgaac
     2701 tggaaactcg ccgtactctg cgctttgaaa tggcgggaaa aagctatttt ctcaaatggc
     2761 atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat
     2821 taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta
     2881 tgtatggggt ggcattcggc gaaaaaggca tgaatccgct gaccagaacc tcgtttatta
     2941 ttaccgaaga tctgacacca accatcagtc tggaagatta ctgtgctgac tgggcgacca
     3001 acccaccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg
     3061 atatgcatgc tgcgggcatt aaccatcgtg actgttatat ctgtcatttc ctgctgcact
     3121 tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgaccta caccgggcgc
     3181 agcttcgcac gcgcgttcca ggtcgttggc gcgataaaga tcttataggg ctttattttt
     3241 cttcaatgaa tattggcctg actcagcggg atatctggcg ctttatgaag gtgtattttg
     3301 ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa
     3361 aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta acttattttt
     3421 gccaaaattt tggatacaga ataaatatgt ctcaactcaa tgatagtgac atcatccttt
     3481 ttgagtataa ttttcattat caaaat
//
LOCUS       DAZPEU010000285         3497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPEU010000285 DAZPEU010000000
VERSION     DAZPEU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.287"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..311
                     /locus_tag="ACHU2L_004003"
     CDS             <1..311
                     /locus_tag="ACHU2L_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR1358617.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACHU2L_004004"
     CDS             468..1217
                     /locus_tag="ACHU2L_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR1358618.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACHU2L_004005"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACHU2L_004005"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR1358619.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHU2L_004006"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHU2L_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR1358620.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3497
                     /locus_tag="ACHU2L_004007"
     CDS             3361..>3497
                     /locus_tag="ACHU2L_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR1358621.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     R"
BASE COUNT          961 a          908 c          864 g          764 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgttt
//
LOCUS       DAZPEU010000286         3485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPEU010000286 DAZPEU010000000
VERSION     DAZPEU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.288"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            147..863
                     /gene="nanC"
                     /locus_tag="ACHU2L_004008"
     CDS             147..863
                     /gene="nanC"
                     /locus_tag="ACHU2L_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR1358622.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            883..1989
                     /gene="nanM"
                     /locus_tag="ACHU2L_004009"
     CDS             883..1989
                     /gene="nanM"
                     /locus_tag="ACHU2L_004009"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR1358623.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2054..3034
                     /locus_tag="ACHU2L_004010"
     CDS             2054..3034
                     /locus_tag="ACHU2L_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR1358624.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3144..3349
                     /locus_tag="ACHU2L_004011"
                     /pseudo
     CDS             3144..3349
                     /locus_tag="ACHU2L_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1029 a          740 c          811 g          905 t
ORIGIN      
        1 aggagacctt atacaaaaaa tataaaatac agttacccgt tgccaaagac actataagcc
       61 tggcaaaaaa atattacaca acataaatgc taattgttta tgcgggcttt gtattgcttt
      121 ctgtatccta caaatgagtg aaatttatga aaaaggctaa aatactttct ggcgtattat
      181 tactgtgctt ttcgtcccca ttaatttctc aggctgcgac actggacgta cgtggtggat
      241 atcgtagtgg aagccacgcc tatgagactc gactcaaagt cagtgaggga tggcaaaatg
      301 gatggtgggc aagcatggaa agtaatacct ggaataccat tcatgataat aaaaaggaaa
      361 atgccgcact caatgatgtt caggttgaag ttaattacgc aattaaactt gatgatcaat
      421 ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc tacggaccct
      481 acgtaaaact gtcctgggac gcgacaaaag atcttaattt tggcattcgc tatcgttacg
      541 actggaaagc ttaccgacaa caagacttat ccggtgatat gtctcgtgat aacgttcatc
      601 gttgggatgg atatgtcact taccatatta atagtgattt caccttcgca tggcaaacga
      661 cgctatacag caaacagaat gattatcgct atgcaaacca caagaaatgg gcgacggaaa
      721 atgcctttgt tctacaatac catatgacgc ccgatattac gccatacata gaatatgact
      781 accttgaccg ccagggtgtt tacaacggca gagataattt atcggaaaac agttatcgca
      841 ttggtgtgtc atttaaactg tagtagacag gagacagtca caatgaataa aactataatg
      901 gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt ctgtattacc ggaaactcct
      961 gtgccattta aaagtggtac cggagcaatt gataacgaca ctgtctacat tggtttaggt
     1021 agcgcaggta cagcatggta caagctagat acacaggcca aagataaaaa atggactgcg
     1081 ttagctgcat tccctggtgg accgagagag caagcaacct ctgcatttat tgatggcaat
     1141 ctgtatgtgt ttggcggcat tggcaaaaac agcgagggat tgactcaggt atttaatgac
     1201 gtacacaaat acaaccccaa aaccaatagc tgggttaaat tgatgtcgca cgcgccgatg
     1261 ggcatggcgg gtcatgtgac ttttgtacac aacggcaagg cttatgttac tggcggtgtt
     1321 aaccagaata tcttcaatgg ctattttgaa gatctcaacg aggctggaaa agattcaacc
     1381 gctatagata aaatcaacgc ccactatttt gacaaaaaag cagaagatta tttcttcaat
     1441 aagtttctgt tgtcttttga tccctcaaca cagcaatgga gttacgctgg cgaatcgccc
     1501 tggtacggaa cggctggtgc ggcggttgtg aataaaggtg ataaaacctg gcttattaat
     1561 ggcgaagcca aaccaggatt gcgaacggat gccgtatttg aacttgattt caccggtaat
     1621 aatttaaaat ggaataagct tgatcccgtc tcatcaccag atggcgtcgc tggcggtttt
     1681 gcggggataa gcaatgattc tcttatattt gctggagggg ccggattcaa aggttcacga
     1741 gaaaattacc agaacggtaa gaactatgcg catgaaggcc tgaaaaaatc atatagcact
     1801 gatattcatc tttggcataa cgggaaatgg gataaatcgg gtgaattatc gcaaggtcgg
     1861 gcctacggag tatcattgcc ctggaataat agtttattaa ttattggcgg tgaaactgca
     1921 ggcggcaaag cggtgacgga ttcagtttta atttctgtga aggataataa agtcacagta
     1981 caaaactaac gcttcagggc cccggtaaag gggccctgct atcaatttgt catttgtatt
     2041 acgcgaaaat tatatgaacg caataatatc acccgattat tactatgttc ttaccgttgc
     2101 tggtcagtct aatgccatgg cgtatggcga aggactgcca ttaccggaca gggaagatgc
     2161 gcctcattcc agaattaaac aattagcgag atttgcgcat acgcatcccg gaggcccgcc
     2221 atgtcacttt aacgacatta ttccactgac tcactgccca cacgatgttc aggatatgca
     2281 gggttatcac catcctctgg caacgaatca tcaaacacag tacggcaccg ttggccaggc
     2341 actgcatatt gcacggaaat tactgccctt cattcctgat aatgcaggga ttcttatcgt
     2401 tccgtgttgc cgtggcggat cggcttttac cgcgggcagc gaagggacat attcagaacg
     2461 gcacggagcc agccatgatg cttgtcgttg gggaacggat actccgctgt accaggattt
     2521 agtcagcaga acgcgagccg cactggcaaa aaatccgcag aacaaattcc tcggcgtatg
     2581 ctggatgcaa ggcgaatttg acttaatgac cagtgactac gcgtcacacc ctcaacactt
     2641 taatcatatg gttgaagcct ttcgtaggga tctaaaacaa taccattctc agcttaataa
     2701 tattactgac gcgccgtggt tttgcggcga taccacctgg tactggaaag aaaatttccc
     2761 gcattcgtat gaagctattt atggcaatta tcaaaataat gttttagcca atattatttt
     2821 cgtcgacttc cagcaacaag gtgaaagagg actgacgaac gcgcctgatg aagacccgga
     2881 cgatttaagc acgggatatt acggttcagc gtaccggtca ccggagaact ggacgacggc
     2941 actgcgaagc agtcatttca gcacggcagc ccgtcggggg attatttctg acagatttgt
     3001 agaagcaatt ttgcagtttt ggcgcgaaag gtgagcgggg agtttattca tgggggatgt
     3061 ccttgttcgt tctcagagta gacaacaaaa aacaaatgag atcaaggtaa tatctcttca
     3121 cttgcgggct cgaacagcag gcaattgcca atataataat ctggcacaag ggtgaaaaac
     3181 gcgcaccgca taaaccgttg ttattgctta cagattagcc ggatgcctta atagacatcc
     3241 gcgccttttc gattatggct cggaaatcta cgaacatcta cacagtttac tggaacgttt
     3301 tgggccacag cgttcgcagc accgtcctga tatgccgttc tggcgattat aagtcgacgg
     3361 attctggcaa atagacgatg atgatatgct taatgatgtt caaagaaatt aggccattat
     3421 tatattattg cttgcatttt ctttcattcc taaaagcacc cgccattaat cccaggcgca
     3481 ttatg
//
LOCUS       DAZPEU010000287         3456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPEU010000287 DAZPEU010000000
VERSION     DAZPEU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.289"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(226..429)
                     /locus_tag="ACHU2L_004012"
     CDS             complement(226..429)
                     /locus_tag="ACHU2L_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358625.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACHU2L_004013"
     CDS             complement(431..586)
                     /locus_tag="ACHU2L_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1358626.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACHU2L_004014"
     CDS             complement(789..1196)
                     /locus_tag="ACHU2L_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1358627.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACHU2L_004015"
     CDS             1273..1500
                     /locus_tag="ACHU2L_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1358628.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACHU2L_004016"
     CDS             1484..1906
                     /locus_tag="ACHU2L_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1358629.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACHU2L_004017"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACHU2L_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="ACHU2L_004018"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="ACHU2L_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAZPEU010000288         3433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPEU010000288 DAZPEU010000000
VERSION     DAZPEU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.290"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..278)
                     /gene="ybgE"
                     /locus_tag="ACHU2L_004019"
     CDS             complement(<1..278)
                     /gene="ybgE"
                     /locus_tag="ACHU2L_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR1358630.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGL"
     gene            complement(278..391)
                     /gene="cydX"
                     /locus_tag="ACHU2L_004020"
     CDS             complement(278..391)
                     /gene="cydX"
                     /locus_tag="ACHU2L_004020"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR1358631.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(406..1545)
                     /gene="cydB"
                     /locus_tag="ACHU2L_004021"
     CDS             complement(406..1545)
                     /gene="cydB"
                     /locus_tag="ACHU2L_004021"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1358632.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(1561..3129)
                     /gene="cydA"
                     /locus_tag="ACHU2L_004022"
     CDS             complement(1561..3129)
                     /gene="cydA"
                     /locus_tag="ACHU2L_004022"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR1358633.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT          965 a          938 c          837 g          693 t
ORIGIN      
        1 atcagcccga caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga
       61 accttctgcg gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc
      121 aataaaccat gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa
      181 aacatacatc ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg
      241 tccattaccg catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt
      301 tgcctgattc gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa
      361 gcgttcccag aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg
      421 ggtgttacgt tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc
      481 ggtgtagagc agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt
      541 cagctggctg gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg
      601 catcacaaac gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga
      661 ggagaacaca aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag
      721 cagaaccaca cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag
      781 ccatgcgcca gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc
      841 cattgtcgat ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc
      901 gaaacagacc agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag
      961 ttcgcccacg gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct
     1021 caccacgcct gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta
     1081 cagacgcaga tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac
     1141 accaattacc agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg
     1201 ccagcgggtt tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga
     1261 cgccagcacg aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg
     1321 ccaggcagca aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg
     1381 tggtgcaatg gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt
     1441 gagcatgccc acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc
     1501 aaccagcagc caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc
     1561 ttagcgtgcc ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag
     1621 gctgcttggg ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag
     1681 ggtatacagg ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga
     1741 attcgccaca gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc
     1801 cacgaaccag cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg
     1861 cagaagccat tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat
     1921 gattgccagc agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc
     1981 tacacgcggg atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac
     2041 gtttggcgta tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa
     2101 ctggtcacga accgtctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc
     2161 cttcatcccg ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac
     2221 cggggtatcc acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa
     2281 tttgttcgtc tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg
     2341 ttgcgtttcc cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc
     2401 gtagccggat tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc
     2461 agcgatagca aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc
     2521 gctgataccg aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa
     2581 tttcacctga gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat
     2641 agtttcaaag ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat
     2701 ccacagtgcg gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac
     2761 tttacccaga cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa
     2821 ggccatcaga ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata
     2881 gtaagaccag ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc
     2941 gaagttgata ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc
     3001 ggagaggacg tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg
     3061 cacaaaaagg aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat
     3121 atctaacatc atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa
     3181 agggtcacac gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat
     3241 gcacacaaaa acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc
     3301 cacgtatata ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg
     3361 catttttcga cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag
     3421 aacaatttat ctc
//
LOCUS       DAZPEU010000289         3414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPEU010000289 DAZPEU010000000
VERSION     DAZPEU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.291"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..238
                     /locus_tag="ACHU2L_004023"
     CDS             <1..238
                     /locus_tag="ACHU2L_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR1358634.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="ACHU2L_004024"
     CDS             248..886
                     /locus_tag="ACHU2L_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR1358635.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="ACHU2L_004025"
     CDS             889..2052
                     /locus_tag="ACHU2L_004025"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1358636.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3414
                     /locus_tag="ACHU2L_004026"
     CDS             2136..>3414
                     /locus_tag="ACHU2L_004026"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1358637.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggtt
//
LOCUS       DAZPEU010000290         3396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPEU010000290 DAZPEU010000000
VERSION     DAZPEU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.292"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            108..2105
                     /locus_tag="ACHU2L_004027"
     CDS             108..2105
                     /locus_tag="ACHU2L_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFR1358638.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..>3396
                     /locus_tag="ACHU2L_004028"
     CDS             2168..>3396
                     /locus_tag="ACHU2L_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFR1358639.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKS"
BASE COUNT          801 a          783 c          859 g          953 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatc
//
LOCUS       DAZPEU010000291         3373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPEU010000291 DAZPEU010000000
VERSION     DAZPEU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.293"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1202
                     /locus_tag="ACHU2L_004029"
     CDS             <1..1202
                     /locus_tag="ACHU2L_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR1358640.1"
                     /translation="TKDGQRLRNMIMSFNADIVARSPKKKPTFFPEQIEGYEYMYGGN
                     DDYPYYLQNRTDENGNDLPIGPISYMENPEVPQANAYMLEAATNAVKEVASLGVDAQA
                     ANGQVAFDTVNQLNMRADLETYVFQDNLATAMRRDGEIYASMVNDIYDVPRHVTLTLE
                     DGSEKDVQLYAQVVDYQSGNVVTLNDIRGRYECYTDVGPSFQSMKEQNRAEIQELLTK
                     VPQGTPEFQMLMLQYFTLLDGKGVEMMREYANKQLVMMGLKKPETPEEMEMVQQAQQQ
                     PQQPSAEQIQAQGILLQGQAELLKAENQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMD
                     LDKQAELREYLRLVGQFQQQRSKDARANAELLLKDADQTHSQRMDFANLMRQVQIPSG
                     GVAETPQ"
     gene            1216..2127
                     /locus_tag="ACHU2L_004030"
     CDS             1216..2127
                     /locus_tag="ACHU2L_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR1358641.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            2127..>3373
                     /locus_tag="ACHU2L_004031"
     CDS             2127..>3373
                     /locus_tag="ACHU2L_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR1358642.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACA"
BASE COUNT          927 a          872 c          872 g          702 t
ORIGIN      
        1 tgacgaaaga cggtcaacgc cttcgtaaca tgattatgtc gttcaacgcc gatattgttg
       61 ctcggtcacc gaagaagaaa ccgaccttct tccctgagca aatcgaaggc tacgaataca
      121 tgtacggcgg aaatgatgac tatccgtact atctgcagaa caggactgat gaaaacggta
      181 acgacctgcc gattggtcca atctcctaca tggaaaaccc tgaagtgccg caagccaacg
      241 cttacatgct tgaggctgcc accaacgcag tgaaagaagt ggctagtctt ggcgtggatg
      301 cgcaggcggc aaatggtcag gtcgcttttg ataccgtcaa tcaactgaac atgcgggcag
      361 accttgagac atacgtgttt caggataacc tggctaccgc aatgcgacgt gatggcgaga
      421 tttatgcctc aatggtcaac gatatttatg acgttcctcg tcatgtaacg ctgacacttg
      481 aagatggaag cgagaaagac gttcaactct atgcgcaagt tgtcgattac cagtccggca
      541 atgtggtcac actcaacgac attcgcggtc gatatgagtg ctatacagac gttgggccat
      601 ccttccagag tatgaaggaa cagaaccgcg cagagattca ggagttgctc accaaggttc
      661 cgcaaggtac tccagagttc cagatgctga tgctgcaata cttcacgctg cttgacggta
      721 aaggcgtcga gatgatgcga gagtacgcga acaagcaact ggtgatgatg gggctgaaga
      781 aaccagaaac acctgaagag atggagatgg tgcagcaggc acaacaacag ccgcagcagc
      841 catcagcaga gcaaattcag gcgcagggta tccttctgca aggtcaggct gaattgctca
      901 aggcagagaa ccaacaggcg cagattcagg ttgaagctgc caaggttgaa gcccaaaacc
      961 aactcaacgc cgcgaagatt gcagaaatat tcaacaatat ggacctcgac aagcaggcag
     1021 aactgcgtga gtacctcagg ctcgtaggtc aattccagca acagcgcagc aaagatgctc
     1081 gtgctaacgc tgagctgctt cttaaagatg cagaccagac tcattcacaa cgcatggatt
     1141 tcgcgaatct tatgcgtcaa gttcaaatcc cctccggcgg agtagccgag acacctcaat
     1201 aagagagagt taatcatgga ccaaaccacc gacattcagg cttctgaaga attaaccctg
     1261 cccggcaatc atgcagcggc atctgctgat ggcttagttg tcgataatgc caacgacagc
     1321 gcaggtcagg aagaaggctt tgagattgtc cggaaagacg atgagaaacc aaaacaagac
     1381 ccggcaacta atgctgaatt tgcccgtcgc cgcatcgaac gcaaacgcca gcgtgagctt
     1441 gagcagcaga tggaagcggt taagcgtgga gagttgccgg agcacctgcg ggtgaaccct
     1501 gagttaccaa aacaaccaga ccctaacgat tatctttccg aagatgcact ggctaagtac
     1561 gactatgacc agagccgcgc actggctgcc ttccagcagg caaacagtga atggcagatc
     1621 aaggctatgg acgcacgaag ccaggctgtc gccgagcagg gtcgcaaaac tcaggagttc
     1681 acccagcaat cagcgcaata cgtcgaggcg gcccgtaagc actacgaggc agcggaaaag
     1741 ctcaatatcc ctgactatca ggagaaagag gatgcattca tgcaactggt gccgccagca
     1801 gtcggtgccg acatcatgcg cctcttcccg gagaaatccg ccgctctcat gtatcacctt
     1861 ggtgctaatc ctgagaaaac acgccagttg ctggcgatgg acgggcaatc cgcgctgatt
     1921 gaactcactc gactgtcaga acgtttaact ctcaagcctc gagccaagcc tgtttcagaa
     1981 gccccgttac ctgatgaacc cattcaggga catgctgttg ctgcaaatat ctctgcgatt
     2041 gaaaagcaga tggaggcggc agcaaacaaa ggggatgtag agacgtaccg caagctcaag
     2101 gcgcaactga ataaaggaat tcgataatgg cattaaatga aggtcaactg gtcacgtatg
     2161 ctctggatga aatcatcgga accgtccaga acctgacgcc aatggcgtca aaagtgacaa
     2221 aatacccccc tccggcagaa tccatgcagc gttcaagcaa caccgtgtgg atgcctgttg
     2281 agcaggaagc gccaacccag actggctggg atttaactgg caacgcaacc gggattctgg
     2341 aactctccgt gaagtgcaac atgggcgatc cggataacga tttcttcgag cttcgtgcag
     2401 atgacctgcg tgatgagcgt tcttaccgtc gccgcatcca ggcatccgcc aaaaaactgg
     2461 cgaataacat tgagtcagcg attgccaaac aggcaactga aatgggctcg cttgttgttc
     2521 acgatacccg cgcaattggt ccatcgactg gcctgcctgg ctgggatttt gtgtctgatg
     2581 cagagcgact gatgttctcc cgtgagctaa accgcgacat gggcatcagt tacttcctga
     2641 accctgacga ttaccgcaaa gcaggccgca acctggtaga tggtgacatc ttcgggcgcg
     2701 ttcctgaaga agcatatcga aacggtacta ttcagcgtca gattgctggc tttgatgaaa
     2761 ttcttcgctc accgaaactt ccggcagtta ccaagtcaac cgcgactggt gtaactgttt
     2821 ctggtgcgca gaagtttaag ccgcaggcat acacccttga taccgatggt aacaaagaga
     2881 acgtcgacaa ccgtgttgca acggtgaccg tatcctccac caccggattt aagcgcggcg
     2941 acaaaatcag cttcattggt gtgaagttcc tgtctcagat ggcgaagaat gtgctgactg
     3001 atgacgcgac tttctcaatc acccgtgtga tcgatggtac tcacatcgaa atcacgccga
     3061 agccgattgc gctggatgac gcgtcactga caaaagaaga gaaggcttac gctaacgtaa
     3121 acacctctct tgctgatacc actccggtaa acgttctgaa cgtggcaaca accaccgcta
     3181 acgtgttctg ggctgatgac tcaatccgcc tgctgtctca gccgatcccg gtaacccatg
     3241 aactgtttgc tggcatgaaa acgtcttcct tcagcattcc aggtattggt gttaacggca
     3301 tcttcgcaac gcagggtgat atcaacactc tgtctggtaa gtgccgtatt gctgtgtggt
     3361 attcagcatg tgc
//
LOCUS       DAZPEU010000292         3348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPEU010000292 DAZPEU010000000
VERSION     DAZPEU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.294"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            19..897
                     /locus_tag="ACHU2L_004032"
     CDS             19..897
                     /locus_tag="ACHU2L_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1358643.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACHU2L_004033"
     CDS             894..2285
                     /locus_tag="ACHU2L_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1358644.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACHU2L_004034"
     CDS             2282..2539
                     /locus_tag="ACHU2L_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358645.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACHU2L_004035"
     CDS             2634..2834
                     /locus_tag="ACHU2L_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR1358646.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACHU2L_004036"
     CDS             2827..3207
                     /locus_tag="ACHU2L_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR1358647.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAZPEU010000293         3333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPEU010000293 DAZPEU010000000
VERSION     DAZPEU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.295"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..149
                     /locus_tag="ACHU2L_004037"
     CDS             <1..149
                     /locus_tag="ACHU2L_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate transporter"
                     /protein_id="HFR1358648.1"
                     /translation="AKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            152..1240
                     /gene="capU"
                     /locus_tag="ACHU2L_004038"
     CDS             152..1240
                     /gene="capU"
                     /locus_tag="ACHU2L_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR1358649.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            1245..2195
                     /gene="virK"
                     /locus_tag="ACHU2L_004039"
     CDS             1245..2195
                     /gene="virK"
                     /locus_tag="ACHU2L_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1358650.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            2260..3204
                     /gene="lpxM"
                     /locus_tag="ACHU2L_004040"
                     /gene_synonym="msbB"
     CDS             2260..3204
                     /gene="lpxM"
                     /locus_tag="ACHU2L_004040"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1358651.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          938 a          635 c          777 g          983 t
ORIGIN      
        1 ctgctaaagg aactacacgg ataggaagaa taagcaccaa agaacgggaa agttgcgcat
       61 ggctgaaaag acgacttttt tattatacaa ccccgttttt ttcatcgttg ctggcctttc
      121 ataaggggcc aagattaccg gatgattaat aatgaatata ctatttacgg aatcatcacc
      181 gaatataggt ggtcaggaat tacaggctgt tgctcaaatg aaggccctga agaaaatggg
      241 gcattcagtt ctgcttgtct gcagggaaaa cagcaaaatt gcttttgaag ccagtaaatt
      301 ggggattgat atcacattcg cgttatttcg aaacagtctt cacatcccta ctgcatggag
      361 attactcgga atagttcatg gttttcagcc caatgcaatc gtttgtcaca gtgggcatga
      421 tagcaatatt gttggtttag tacggttatt cactaggaaa catccattca gaattatcag
      481 gcaaaagaca tatttgacac gaaaaacaaa agttttttca ataaatcatt tttgcgatga
      541 ggtgattgtt cccggaacaa gtatgaagac acatctggag caggaaggat gtcgaacccg
      601 ggttactgtt gtgcctccag gctttgattt ccagaaatta tacgttgatt cccgaaacag
      661 tttgcctcca aatgttcttt cttggctggc gtcccgaagg ggatgccccg ttattgctca
      721 ggtaggaatg ttgcgcccgg aaaaagggca cgaatttatg ttgaatttac tgttccattt
      781 aaaaatgaat ggacgacagt tctgttggtt gattgtgggg tctggttcgc ctgaactgcg
      841 ggagcattta cagtatcaga ttgacagtat gggcatgcat gatgatgttt ttattgctga
      901 caatgttttt cctgccgccc ccgtatatcg ggttgccagt ctggtggttc tgccttcaga
      961 aaacgaatcg tttggtatgg tactggcaga agcatcggca ttttctgtgc ctgtactggc
     1021 cagtcagatt ggtggaatcc ctgatgttat tcagaataac cagaccggga cattgttacc
     1081 agcaggtaat aagcacgcat ggatgtgcgc cctgaatgat ttttttaatg accctgggcg
     1141 tttttatcag atggctcgcc aggcaaaaca ggatatagaa gagcggtttg atattaataa
     1201 aactgcgtta aaaatactca cattagcgaa gcacaagtaa caatatgttt tctgtaagta
     1261 acttatcatt tatcggtttc cttaaaagga ttgttttttc ctcagattca ctcccgggga
     1321 agtgggaaca cagaaaattc cggttcatgt acattttgcg atgtgctata aatccggttg
     1381 ccagtattcg atattattac gaactgcgtt ccttgcagtg cattgaggat attctggcaa
     1441 tacaacccac gttgccagca agaattcatc gaccttattt gcataaaggg gggcgagcct
     1501 ggtcccgggg gcaatatatt cttgagcatt atcgctttgt gcaaaatttg ccagaaaaat
     1561 attctgaatt tttgtttcca caaaaatcgg tatcattagt ccaattcatc gggaaagatg
     1621 gagaggattt tgatatccag tgttctccca gtggttttga ccgggaagga gagttgatgt
     1681 tgtcattgtt tttcaataaa atagtgattg cccggttaac attttcagtc attctgactc
     1741 aaaatggaca caccgctttt attggcggat tacagggagc ccccaaaaat accggacctg
     1801 atattattcg gtgcgcaacc cgggcctgtt atgggctttt cccaaaacgg attatttttg
     1861 aagcattttg tgcgttgatg aaagcttgta atgtctctga gtgtctggca gtcagtgagc
     1921 acagtcatgt tttccggcaa ttgagatact ggtatcagaa acgcaaaacc tttgttgctg
     1981 tctacagtga tttctgggag tctgtagcag ggaaaacctg tggtgactgg tacaaattac
     2041 caacacaggt ggttcgaaag ccgctaagca atatagctag taaaaaacgc tctgagtatc
     2101 gaaaacgata cgctttgctg gattatatcc atgaaactgc tatccgctct ttggatgcat
     2161 atcctgtaaa ctcagaacat caggacttaa attaaggttt gatgtattct ctgaataaaa
     2221 tattaatgat gattttggta ggggcattcg cactaaataa tgaaaaaata caaatccgag
     2281 tttattcctg aatttaagaa aaattatctt tcccctgttt actggtttac atggttcgtt
     2341 ttgggaatga ttgcaggtat ttcaatgttt cccccttcat tcagagatcc tgtcttggcc
     2401 aaaatagggc gttgggtggg cagattgagc agaaaagctc gtcgcagggc gacgattaat
     2461 ttatcgcttt gtttcccgga aaagagtgat acagaacggg aaataattgt cgacaatatg
     2521 tttgccacag cattgcaatc catagtgatg atggcagaac tggcaattcg tggtccggaa
     2581 aagttccaga aacgagtgtt ctggaaaggc ctcgaaattc ttgaggaaat tcggcacaat
     2641 aacagaaatg tgatttttct ggttccccat ggctggagcg tggatattcc tgcaatgttg
     2701 ctggcagctc agggggaaaa aatggccgcc atgtttcatc agcaacgaaa tccagtgatt
     2761 gattatgtct ggaattctgt acggcgtaaa ttcggggggc gcttacattc ccgggaggat
     2821 gggataaaac catttattca gtcagtacgc cagggatact gggggtatta ccttccagat
     2881 caggatcatg gtcctgaata cagtgaattt gctgattttt ttgcgaccta taaagcgaca
     2941 ttaccaatta ttggacgtct gatgaacatc agtcaggcta tgattatacc gcttttcccg
     3001 gtttatgatg aaaaaaaaca tttcctgact attgaagttc ggccaccaat ggatgcatgc
     3061 attgccagcg cggacaataa aatgattgcc cgacaaatga acaaaacagt ggaaattttg
     3121 gtggggtcac atccggaaca gtatatctgg gttttaaaat tgttaaaaac gcgcaaatca
     3181 aacgaagcgg acccgtaccc ttgaaattat aaagtacagg tatttccact agttgtttct
     3241 tacaggttac caatcgaaac acatccccct tccgtgtgtt gcccgtttgg cacggaggac
     3301 tgtgtagatg cagttcgtta aatttaattt atg
//
LOCUS       DAZPEU010000294         3326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPEU010000294 DAZPEU010000000
VERSION     DAZPEU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.296"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..555)
                     /gene="yjgL"
                     /locus_tag="ACHU2L_004041"
     CDS             complement(<1..555)
                     /gene="yjgL"
                     /locus_tag="ACHU2L_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFR1358652.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARL"
     gene            complement(678..1130)
                     /gene="tabA"
                     /locus_tag="ACHU2L_004042"
     CDS             complement(678..1130)
                     /gene="tabA"
                     /locus_tag="ACHU2L_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1358653.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1275..1868)
                     /locus_tag="ACHU2L_004043"
     CDS             complement(1275..1868)
                     /locus_tag="ACHU2L_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1358654.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            1939..2652
                     /gene="bdcA"
                     /locus_tag="ACHU2L_004044"
     CDS             1939..2652
                     /gene="bdcA"
                     /locus_tag="ACHU2L_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1358655.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2784..3179
                     /locus_tag="ACHU2L_004045"
     CDS             2784..3179
                     /locus_tag="ACHU2L_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1358656.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
BASE COUNT          875 a          804 c          738 g          909 t
ORIGIN      
        1 tagacgcgct attttcttgt tagcttcgcg ttggtaacac gccagaacct tgttagtaat
       61 caggtgtaat gaactatcat cttttctttc aaaagacttc ccttcataac gattagtcga
      121 accatcagat tgtttaaata taatgactgt atgtccttca cgaatccctt tagtagaagc
      181 tgaggcaagt gtaatttcta tcttcacctt agataaatcc gctcgctccc cttcaggatt
      241 attcatgtaa aacttcattt tttcattgcc atcagtatct ttagaataag atgagaactc
      301 aatatgagcc atcatatttc tacccgcttc gttggagcgt ttgctaaaat ccacataacc
      361 cagggtaaat atctttgcca aaaactccaa aaatgctcta ggagaaagaa tattgcttct
      421 gctattctgc cccccggcaa ttcgggcaaa atcattcatt cccacatacc aggtatctaa
      481 ggcttcattt ttttgtttaa aattgtcggc taatgtaatg tgttgagaat aattcaaatc
      541 tgatattttg cccatgacga actccttgag ttaataagaa cgtctaagca ttacaaattt
      601 tactcacaaa aaaatatcaa aaaacgctct tttcacaaac tcgccgtaaa cctggagtta
      661 tttacggcga aaagtcatca cgccatcagc atcttcacta ctgctttgcg aacctgggct
      721 ggcgcgccca ctgcgcacag cggtttatgc acttcccccg gataaaacac aacaaaatca
      781 ccttcattta ggataactgt tttctcatca acgccttccg gcaaaaatgc gatgtcttta
      841 tcagctaacc agtcggtatc cggcgcgcct gcaggttgcg tgctgaaggt catgccttcc
      901 tgacctttta acacaatctg aatgtcaaga tagcgggcat ggtactccgc acggcgagct
      961 tcgtacggct cggtcatatc ttccgagata agataaaaca gacgattgcc ttcgatatca
     1021 tgcttgccct ttggcgtttc tgccgtaacg tgtgctttga tatgctcaat cgcctggcgt
     1081 aactcctggg gtagccacgg ctgaagatta tgaatatttc cgatgatcat cttttccact
     1141 ccttaattaa aacaatgttt catttctatc gttatacgaa aagatgatca attccgccag
     1201 ccttaacgcg tgaattattg tcttatcaca acttatcggc aggcgcgttc ccgcctttcg
     1261 gatcaaggcc agcatcactc cttgagaaga gtttttatcg cttcccctgc cagggcagct
     1321 gttgcacaga gttgttcgat ggagtgcccc tcccgtgcct tcgcagaaag ccctgacatc
     1381 acggtactca taaaatcagt cacacattgt gcgctttgtg gatgccgcct ggcgatatag
     1441 tcataaatgg tcgtttctgc ggcgtgataa tattgaatgg caatatcacg cgcttgtgga
     1501 tcatgactat gaataccttc aagaaccata cagccagcgc agccgccgtt ttggctatat
     1561 ctgcgcgccg cttcttttaa tacctcaacc aggcactcgc ctaccggacg atcatcacga
     1621 agaatatcgg caagcggaat agcttccgta ccgacatatt cattgagtac acggctaaat
     1681 aacccagcct tactgccaaa agccgcgtag agactcgggg ggttaatacc aagataatca
     1741 gtaacttcag agacacttac tgcatcgaaa cctttttggt gaaaaagcac ttttgccgca
     1801 gagactgcct gctcaggagc gaaacgtctg ggacgacctg gaacgcgaga ttgtttttta
     1861 gtgaccatta caaaacctgt tgacagaaag ttaaaacagt tttgtaatgc atgttacata
     1921 ataaatcaag gagtccttat gggcgctttt accggtaaga cagttctcat cctcggtggc
     1981 agtcgtggaa tcggtgccgc tatcgtacgt cgtttcgtca ccgatggggc caatgtacga
     2041 ttcacctatg cggggtcgaa agatgccgct gaacgtctgg cacaagagac tggagcgaca
     2101 gcagtattca cagatagtgc tgacagagac gctgttattg atgtcgttcg taagagcggc
     2161 gcattggata tcctggtggt aaatgcaggt attggcgtct ttggcgatgc cctggaatta
     2221 aatgccgacg atattgatcg ccttttcaaa atcaatattc atgctcctta tcatgcctct
     2281 gttgaagccg cccggcagat gcccgaaggc gggcgcatct taatcatcgg ctccgtgaat
     2341 ggcgatcgta tgcctgttgc aggcatggct gcttatgccg ccagtaaatc tgccctgcaa
     2401 ggcatggcgc gcgggctggc ccgtgatttt ggaccgcgag ggatcaccat caacgtcgtc
     2461 cagccaggac caattgatac cgacgttaat cccgccaacg ggccaatgcg cgatatgttg
     2521 catagtttta tggccatcaa aagacatggg caaccggaag aggtcgctgg tatggtcgca
     2581 tggttagcag ggccagaagc cagttttgtt accggcgcga tgcataccat tgatggcgcg
     2641 tttggcgcat aaccgactac gctcaattaa gcccagccat tatccatgat gtctgggttt
     2701 tgtttactaa cgtcgtcccc ctaaaagcgg ctcccgataa atataaatct tctgttcatg
     2761 tcgtaaatct tgaggataac caaatggtag aaagaaccgc tgttttccct gctggccgac
     2821 attcactgta tgctgagcat cgttattctg cggctattcg ttccggcgat ttgctgtttg
     2881 tttccgggca agtaggaagt cgagaggacg gaacaccaga acccgatttt cagcaacaag
     2941 tcagactggc ctttgataat ttgcatgcga ccctggcagc tgcgggatgc acttttgacg
     3001 atatcattga tgttacgagc tttcataccg atccggaaaa acaatctgaa gacatcatga
     3061 cggtgaaaaa tgaaatattt agcgccccac cttatccaaa ctggacggcg gtgggtgtta
     3121 cctggctggc aggctttgat tttgaaatta aagtgatagc gcgtatccct gagctataag
     3181 caatagtgtt agccgttcgt tttcaccctc cgccctataa gtcggatgaa tggaataaaa
     3241 tgcatttctg attgcgtgaa aatgaaaaag aaaaaagcag gaaatgtctg caattattga
     3301 taccgaacga cacttcccct ccagaa
//
LOCUS       DAZPEU010000295         3325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPEU010000295 DAZPEU010000000
VERSION     DAZPEU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.297"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHU2L_004046"
     CDS             <1..208
                     /locus_tag="ACHU2L_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358657.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            269..457
                     /locus_tag="ACHU2L_004047"
     CDS             269..457
                     /locus_tag="ACHU2L_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358658.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            454..1281
                     /locus_tag="ACHU2L_004048"
     CDS             454..1281
                     /locus_tag="ACHU2L_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1358659.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="ACHU2L_004049"
     CDS             1325..2074
                     /locus_tag="ACHU2L_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1358660.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="ACHU2L_004050"
     CDS             2071..2640
                     /locus_tag="ACHU2L_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358661.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2637..2759
                     /locus_tag="ACHU2L_004051"
     CDS             2637..2759
                     /locus_tag="ACHU2L_004051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358662.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2764..3006
                     /locus_tag="ACHU2L_004052"
     CDS             2764..3006
                     /locus_tag="ACHU2L_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR1358663.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="ACHU2L_004053"
     CDS             3010..3156
                     /locus_tag="ACHU2L_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358664.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHU2L_004054"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHU2L_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR1358665.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          797 c          854 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAZPEU010000296         3280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPEU010000296 DAZPEU010000000
VERSION     DAZPEU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.298"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..263
                     /gene="yobA"
                     /locus_tag="ACHU2L_004055"
     CDS             <1..263
                     /gene="yobA"
                     /locus_tag="ACHU2L_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR1358666.1"
                     /translation="AQVTAAPQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNE
                     QDQKQLIVPLADSLKPGTYTVDWHVVSVDGHKTKGHYTFSVK"
     gene            267..1139
                     /gene="copD"
                     /locus_tag="ACHU2L_004056"
                     /pseudo
     CDS             267..1139
                     /gene="copD"
                     /locus_tag="ACHU2L_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            1152..1493
                     /locus_tag="ACHU2L_004057"
     CDS             1152..1493
                     /locus_tag="ACHU2L_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR1358667.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1886..2963)
                     /locus_tag="ACHU2L_004058"
                     /pseudo
     CDS             complement(1886..2963)
                     /locus_tag="ACHU2L_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2929..3210)
                     /locus_tag="ACHU2L_004059"
     CDS             complement(2929..3210)
                     /locus_tag="ACHU2L_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1358668.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT          783 a          750 c          751 g          996 t
ORIGIN      
        1 acgcgcaagt gacagctgcc ccgcaggcaa tcactttaaa cttctcggaa ggcgttgaaa
       61 caggattcag tggtgcaaaa atcacggggc caaaaaacga aaatattaaa acattaccag
      121 cgaagcggaa tgagcaggac caaaagcaac tgattgttcc actggccgat tcgctgaaac
      181 ccggaaccta caccgttgac tggcatgttg tttcggtgga cgggcataaa acgaaaggac
      241 actacacctt tagcgtgaaa taaatcatgc tggcgtttac ctggatcgcg ctgcgattta
      301 tccatttcac ctcgttgatg ctggtctttg gctttgcaat gtatggtgcc tggctggccc
      361 ccttaatgat tcgtcgcttg ctgacgaagc gttctctgcg tttacaacag cacgctgccg
      421 tctggagttt gatcagtgcc actgcaatgc tcgccgttca gggcgggttg atgggaacgg
      481 gatggacaga tgtattttcg ccaaacattt ggcaggctgt tttgcaaacg cagtttggtg
      541 gcgtctggtt atggcaaatt gttctcgccc tcgtaacgtt gatcgtcgcc cttatgcaac
      601 cgcggaatat gccacgtttg ctgtttatgc tcactaccgc gcaatttatt ctgctggcgg
      661 gagtggggta tgcgacgctg aatgaagggg taacagcgaa aatccatcag actaatcacg
      721 cgattcacct gatttgtgcg gcggcgtggt ttgggggatt actgccggtg ctctggtgta
      781 tgcaactcat caaaggccgt tggcgacact aggctattca ggcgctgatg cgtttttcct
      841 ggtgcgggca ttttgcggtg atcggcgtac tggcaagcgg cgtgcttaat gcattgttga
      901 ttaccggatt tccccccacg cttacgactt actggggcca actcttattg ctcaaagcca
      961 tcctggtgat gattatggtg gtcattgcgc tggctaatcg ttatgttctt gtaccgcgca
     1021 tgaggcagga tgaagatcgt gcggcaccct ggtttgtgtg gatgactaaa ctcgaatggg
     1081 cgataggtgc ggtagtgctg gtgatcatca gcctgcttgc gactctcgaa cctttttgat
     1141 ggattacgaa aatgatgaaa aaaagtattc tggcgtttct gttactcacc agttctgcag
     1201 cggcgctggc ggctccacag gtgataaccg tgagtcgttt tgaagtgggt aaagacaaat
     1261 gggcgtttaa tcgcgaagag gtaatgctga cttgccgacc gggtaatgct ttgtatgtca
     1321 tcaacccaag taccctcgtg cagtatcctt taaacgatat cgcacaaaag gaagttgcca
     1381 gtgggaagac caatgcccaa cccatttcgg tgattcagat tgatgatcct aacaatcccg
     1441 gcgaaaaaat gagtctggca ccgtttatag aacgagctga aaaactctgt taattaccta
     1501 aaatagcctt ttgatttcca ataaaaaaac cgcctcagtt ctttcaccag aacgggcggt
     1561 ttttaacatt taagctgatg agcaccgcgc tttttattga ccattttgca cgcaaactgg
     1621 aaaacctggc gtcgtcatct attcttaaag ggcaaggcaa ctaagcctgc attaatgcca
     1681 acttttagcg cacggctctc tcccaagagc catttccctg gaccgaatac aggaatcgta
     1741 ttcggtcttt ttttaattgt atttaaaatc aattagttgc aaacgtctcc ccgaaattcc
     1801 ccgaaattta ctcgaatttc tgtattccgg tcttttttgg ttatatcaca accaaaatac
     1861 atttaacaat ccatttacgt taaaatcaga gcagtaagta cgttttttct ctctcatcaa
     1921 gatacatttt tgttgtcttc tccgatgtgt ggccaagtag acgctgagca aattcttctc
     1981 cacatatttc tttgtacaat cgtccagcca ggcttctgat ttcgtgaaaa gttggtgggt
     2041 tttcactgaa cagtgatacc tgttaatttt ctggctgcga caaatttctt tgtcaggccg
     2101 tccgggtgaa tgctgccgtc agggctgttt tttctaatcc cggcactgat tagataatcc
     2161 cccctactta ccatgcggca ctgttcaact actgcaccaa gtcgtaaacc agcgacagga
     2221 aggctgagtg acagggggat agcaatcatc attcctgtct taatttgcct gatgtggaga
     2281 cgatcatcat aaatatcact gaaccgcata ttcgttatgt cttcgcgacg ttgtcctgtt
     2341 acaagggcta agtccatagc taatgggaac catgccggaa gttgatctgc tgcctccctg
     2401 atgcagttgt atgtctttag tttcagccgt tctcttgtaa ctactatttt cggtgctctt
     2461 gttggcgtta ctggattttg agatatacgt ccttcaacaa tggcttcgcg gaacatatca
     2521 gataacacag aacgcattga tcctgccatc gtgtttttcc ctccttcaat ctacaaatca
     2581 agaaactcgg cgatatggcg agtggttatt tctgtcagta aaacccctcc taatttttct
     2641 tttattgtct ccagttgatt tacccgagtt ttataagtat ttctggacgc ttttctcctt
     2701 ataagaatcg ttttgtaacg ttcaatccag cctgccatag taaatgagtc gaccccttta
     2761 agcttttcaa ttagggcagc aggagagtag tttttgtata tataatgatt tgcttcaatt
     2821 gcctgcgcta ctgcatctct tcttgaaatt ttacctagtg taaattcttc tttcgtcaga
     2881 gggttgcgcc agtaatatgc tttgtctctc cttcgatatg ttaagtttct aggcaaattg
     2941 ggatcgtatt ttttccgctg catgttttaa cttctccatt aacggactgt ctctcccttg
     3001 tcgcccatta ggctgatggt gtgttatatc ggtatcaacc ttatttgggt tgatatagaa
     3061 agcctccgga accaccctgt aactcctccc gtgtagttca ggtgcaggat aaatgtttcc
     3121 attccttgcc catcgtctca gcgttgatat tgatggtggg ttatccggat atctgagttt
     3181 tccccacgtt ttgagtgtca caagattcat tgccatacct ctcacgatat gaccgccagt
     3241 aaatatacag aatactggcg ggtgtggttg atttttaata
//
LOCUS       DAZPEU010000297         3219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPEU010000297 DAZPEU010000000
VERSION     DAZPEU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.299"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            15..1055
                     /gene="gatD"
                     /locus_tag="ACHU2L_004060"
     CDS             15..1055
                     /gene="gatD"
                     /locus_tag="ACHU2L_004060"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1358669.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
     gene            1161..1934
                     /locus_tag="ACHU2L_004061"
     CDS             1161..1934
                     /locus_tag="ACHU2L_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1358670.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2016..2915)
                     /gene="yegS"
                     /locus_tag="ACHU2L_004062"
     CDS             complement(2016..2915)
                     /gene="yegS"
                     /locus_tag="ACHU2L_004062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR1358671.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNSLIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT          854 a          716 c          809 g          840 t
ORIGIN      
        1 aatcaggagt ttttatgaaa tcagtagtga atgatactga tggtatcgtg cgcgttgcaa
       61 aaagcgtcat tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg
      121 gcttatgtgg ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa
      181 cgttaggcca tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac
      241 accctggcga tgcggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtt
      301 tgaaagggtt ttattcccag tgcgcaaaat atgattttat tggctcgcgg cgtgatggtg
      361 gatttgctga atatattgtc gttaagcgaa aaaatgtctt tgctctaccc acggatatgc
      421 ctattgagga tggggctttt attgagccga ttaccgttgg cctgcatgct tttcatttag
      481 cgcaaggttg tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg
      541 ctattcagtg cgctgtcgcg ctgggagcaa agagtgtgac ggcgatcgac attagctcag
      601 aaaaactggc actggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga
      661 gcgcgccgca aatgcagagc gttttacgcg aactgcgctt taatcagctt atcctcgaga
      721 cggctggcgt accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac
      781 tggcgctggt gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa
      841 tattgcgtaa agagctgacg gttatcggca gttggatgaa ctattccagc ccttggccgg
      901 ggcaggagtg ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat
      961 taatcgctca ccgtggaagc tttgaaagct tcacccaggc ggtgcgtgac atcgctcgta
     1021 atgccatgca gggcaaagtg ttgctcattc catgaaaccg cgggccagcg tgatgctggc
     1081 ccggtattgt gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa
     1141 ttgaatatgg gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag
     1201 tgaatgaaca gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag
     1261 cgacaatccg tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg
     1321 gcggtgcggc gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta
     1381 aagagcgatt tcagctcgcc agcgcgccaa aaaacagaat agcgcaggcg gcagtcaaaa
     1441 tgatccatga agggatgacc gttattctcg acagcggaag taccacaatg cttatcgccg
     1501 aaggattaat gaccgctaaa aatatcacgg tgattaccaa cagtctcccg gcagcgtttg
     1561 ccctttccga aaataaagac attactctgg tcgtctgtgg tggcacggtc cgccataaaa
     1621 cgcgctcgat gcatggttcc attgccgagc gttcattgca ggatattaat gccgatttaa
     1681 tgtttgtcgg tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt
     1741 attcgattag cggggcgatg gtcaccgccg caaataaagt gattgccgtt ctcgattcat
     1801 cgaaatttaa tcgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattattg
     1861 ttaccgatga tgctgtgtca gaggtggata aattggcatt gcagaagaca cgggtaaaat
     1921 taattacggt gtagtattgt tctgtatgcc ggataaggtg tttacaccgc atccggcatt
     1981 acggattctc cctggttcaa gagagaatag tttgattaac gcaataatgg acaatctggt
     2041 ggtaatcgac aacgcaacgc cgccggaagt atttcaatat ggaagttttg cccactcaac
     2101 ggttcgccat caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa
     2161 gcgccttcga taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga
     2221 agaatttcat cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgagttc
     2281 gggcacaatt gttgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg
     2341 tcaccttgcc agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc
     2401 atgcgcatta agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc
     2461 ggcgtttctg tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt
     2521 tgtttgttga cctgcgccat atctatcgca atggcattac cggcaattgc cagtttcagc
     2581 gccttatcca gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat
     2641 ggcaaaattc ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact
     2701 tcattaatgg tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc
     2761 tcctctacaa atcgtgcggc atcacctttc tcccaggtga cccgcacatg gatcgtcatt
     2821 ccttcctcac gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttacca
     2881 ttaagaatca gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt
     2941 ctgtagatag tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg
     3001 ataaaaagtc agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg
     3061 acagcgaata ctaacggctg ccaccgataa atttcaaaaa agagcatata cctaatattc
     3121 aactaaacag tggcatcttc aatataatat attaaagccc ccatggagtt accctgaagg
     3181 gcctcaatgt ccgtaattcc tacttatgta ggaaatgtt
//
LOCUS       DAZPEU010000298         3204 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPEU010000298 DAZPEU010000000
VERSION     DAZPEU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.300"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..1409
                     /locus_tag="ACHU2L_004063"
                     /pseudo
     CDS             1..1409
                     /locus_tag="ACHU2L_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(2139..3128)
                     /locus_tag="ACHU2L_004064"
     CDS             complement(2139..3128)
                     /locus_tag="ACHU2L_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358672.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
BASE COUNT          804 a          833 c          841 g          726 t
ORIGIN      
        1 caaaaatgac cgaatggtgg gcatcagccg cagaacactc gttaaatcaa ccgcgacagg
       61 ttctctggcg ctggctgcag gcggtttttc tttaccgttc accctgcgca gtgcagcggc
      121 agcggtacaa caggccagcg aaaaagtgat ctggggttcc tgttccgtca actgtggtag
      181 ccgctgtgca cttcgtctac atgttaaaga taatgaagtg acctgggtgg aaactgacaa
      241 taccggcagc gatgagtacg gcaaccatca gggtacgcgc ctgtttgcgc ggtcgctcca
      301 tccgccggcg tattaatcat cccgatcgct tgaatgaccc aatgaaacgc gtgggtaaac
      361 gcggtgaagg caaattcgaa cggattagct gggatgaagc cctggatact atcgccagta
      421 gcctgaaaaa aaccgtcgaa caatatggca atgaagctgt atatattcag tactcttcgg
      481 ggattgttgg cggcaatatg acccgctctt cgccatcagc ctcggcggtc aaacgcctga
      541 tgaactgcta cggcggttca ctcaatcagt atggctccta cagcaccgcg caaatttcct
      601 gtgccatgcc ctacacctac ggcagtaatg atggcaacag caccacggat attgaaaaca
      661 gcaagctggt cgtgatgttt ggtaacaacc cggcagaaac ccgcatgagc ggtggtggca
      721 tcacttatct tcttgaaaaa gcgcgcgaga aatcgaacgc caaaatgatt gttatcgatc
      781 cgcgatatac cgatacggct gccggtcgtg aagaggaatg gctccctatt cgcccgggca
      841 ccgatgccgc gctggttgcg ggtattgcct gggtattgat taacgaaaat ctcgttgatc
      901 aaccttttct cgataaatac tgcgtcggct atgacgaaaa aaccttaccc gcagatgcac
      961 ccaaaaatgg tcactataaa gcctatattc ttggtgaagg tgacgataaa acagcaaaaa
     1021 cgccgcagtg ggcttcgcaa attaccggta tcccggtaga ccgtatcatc aaactggcgc
     1081 gtgaaattgg cacaacaaaa cccgcttata tctgctaggg ctgggggccg caacgccagg
     1141 caaacggcga gctgactgca cgcgctattg ctatgttacc tattttgacg ggcaatgtcg
     1201 gcatcagcgg cggaaatagt ggcgcgcgtg aatcgaccta taccattacg atagaacgcc
     1261 taccggtgct ggataatccg gtcaaaacgt caatctcctg cttcagctgg acggatgcta
     1321 tcgatcatgg cccgcaaatg acggcactcc gcgacggtgt ccggggcaaa gataaactgg
     1381 atgtgcccat taagttcatc tggaactaca aggacttgaa gcggaccctt agtaggcctg
     1441 aggtcctgca ttgagtcaag accttgtgtc attctttgtg ccagacttgt gtcattagac
     1501 gggatgtcta gacggctggt caggaccctc cggtggcctc ggtctggact tgaggcgggc
     1561 tatgggggaa gtctgctggc ttaatgtatt agatggtcat tcacattttt ctgtcagaaa
     1621 ccatcggtcc cactgtgagt acaaacctaa gcccccagaa gcccacccac agccttagac
     1681 agagtgtcga atacattcac accgagtcga ccggagagga ccatatagag gcccacagag
     1741 aggaacacga gtttatagac cagagtcagg agggacctta ggtcttcgga agtcatggac
     1801 ataaagcccc ccctatcttc tattgcacta ccgcactaga caactagtac aaaacaaagg
     1861 cccagaccat tggtccagac cgtagttagt taatcacagt gacaagccac cacctgaagt
     1921 cctgctgata ttcagtccag acttacctct gctcatggtc ttacctccct cggtctcctg
     1981 accttgggta tcgactacag cttgctgagc gtcatctgga ggagctacag gaggagctac
     2041 aggaggcggg accgaagccc caccttgagt cccaccacca cctgtcaggc ccttggcgat
     2101 ggacctgaag cctttactaa tgctcttacc cattggtctt acgcttcggt cttggtgaac
     2161 ttcaggcgtt tgactacagg caggacggca gtagctgagc cagtgcccat cttgatggtc
     2221 gtagcagctt cagcgacaac gaacacttta ccgaggattt ccaagtcgtg ggactgagag
     2281 tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt cgtgacggaa gcagccgaag
     2341 ctcagggccg tgaagcgttt agccagcatg aatacaccct tgtcttcacc gttgtaatag
     2401 gcgatgtctg tccccagcat atcgacgata gcgtcttcac cggagaagcc ttgcttagca
     2461 gccagacggg acagtgcttt cagttggctc tcatgagcga taataccgta gtcagacacc
     2521 tcagaaccga aagaagactc tgtgttctca tacagagcgt ccatgatagc gtctgcctta
     2581 gcggtcaggc taccagtcag gacaggaagc tctacggtct tcagcagctt agaggattcc
     2641 agaacgtcga ccatgcgagc gttgatggta cggaccacat tctcacagat gttcatagcg
     2701 tcctgctgaa gtgtgccctg agtgaatccg gtggatgact tggcgtccca gagcttggtc
     2761 tgctcagaga tgagaatagc ctgctcgatt ggttcgacca cgatttcttg tccagacgga
     2821 acggtcacga cttcaggtcg cttgccacca cggacaggaa cgaccacaga gcggtcagcc
     2881 agagtgtgag caatgattgg ggtcggtaag ctgttacggg tcggcaggac ttctacaggg
     2941 aacgggatgt tctcttgagc gtctttaagt acaccgttca gcatagcctt aaccgtctta
     3001 gctaactgga gggcatagac ctgcgggtca gcgttagtcg gcagaccttc attgacctga
     3061 atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa actggaaagc gtctttaatc
     3121 gtagtcataa taatcaatct cctaaattag tttaaatact gtgagtcctc aaaactgagg
     3181 agtccctaac atcgggtcag ggac
//
LOCUS       DAZPEU010000299         3179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPEU010000299 DAZPEU010000000
VERSION     DAZPEU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.301"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHU2L_004065"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHU2L_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR1358673.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACHU2L_004066"
     CDS             complement(1772..1906)
                     /locus_tag="ACHU2L_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1358674.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACHU2L_004067"
     CDS             complement(2552..2722)
                     /locus_tag="ACHU2L_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1358675.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAZPEU010000300         3140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPEU010000300 DAZPEU010000000
VERSION     DAZPEU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.302"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(204..1385)
                     /locus_tag="ACHU2L_004068"
     CDS             complement(204..1385)
                     /locus_tag="ACHU2L_004068"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR1358676.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            1640..2869
                     /locus_tag="ACHU2L_004069"
                     /pseudo
     CDS             1640..2869
                     /locus_tag="ACHU2L_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT          831 a          767 c          660 g          882 t
ORIGIN      
        1 gcccatgggc tagggttttc aattaaaaat aaaacaatgt tttattttta atttaaggat
       61 taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg agggagccca
      121 gaaaatgtat gtcgacttgc cagccatcat tattcaacag attaatgacg gcagatattt
      181 atcggtaagg aggaaatgca gagctactcg tcacgttcaa tagtcaatgc cacgccctgg
      241 cccccgccga tacaaagcgt tgccagccct ttgcgggcat tacgtttcac catttcatga
      301 accagagaaa ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg
      361 ccaccattga cattgacccg acgctcatcc cactcaagca tcttgccaac cgaaagcgcc
      421 tgtgcagcaa acgcttcatt agcctcgata agatcgacat cagccaactg ccagcctaca
      481 cgctccaggc aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc cggatctaca
      541 ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta aattcaacgc tcgtgctttg
      601 gcttcgctca tcatcattac cgcagctgcg ccatcgttta tggatgatgc attccccgcg
      661 gtcacagaac cgagactatc aaatgaagga tttaaacggg ctaaaccttc tgcactggcg
      721 tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct gaccgttact ttggatcatt
      781 accgggacga tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc
      841 gagctaagtg cgtaagcatc ctgcaactga cggctgatgc catattcgcg agccagattt
      901 tcggcggtga caccaatatg ataatcattg aaggcatccc acaacccatc atgcaccaga
      961 ctgtcaacca actggctatt gccaagctgt tcaccggtgc ggctatcagt cagaacatgt
     1021 ggtgcgcggc tcatgttttc ctggccacct gcgatgacaa tatcagcctc gccacactgt
     1081 atcgcctgag tagccagatg cagtgcttta agaccggaac cgcaaacgtc attaatcgtg
     1141 attgcagaaa cgctgttagg aagaccacct ttaatagccg attgccttgc cggattctgc
     1201 cctgcccctg cagtcaacac ctgaccaaga attacttcat ccaccgcata tgcaggaacg
     1261 ccggtacgtt ctattaacgc tttcacgacc agactaccaa gttccacggc ggaatgaccc
     1321 gctaacgcac cacgaaagca gccgataggt gtccgtaacg ccccgacaat cacaacgtct
     1381 ttcatcacaa cctcgcacca attaacagtg caatagtaaa tgattgttaa ttacagttat
     1441 cttaattgtt taaaaaaagt gatttttatc acaaaggaaa tatgcctgag cagtagtcag
     1501 agtcataact ggcacgtaag gtttgcaacc actaacccac caatagatgg gtagataggg
     1561 cgttaatctc ccatacttaa cttggtttat ggtaaattgc cctccatttt gtttaatttg
     1621 tagatgatac gttcagataa tgtctaatat ttggtctaaa gaagaaactc tgtgaagttt
     1681 cgcgctctat ggcacagccg ttgatgcagg cacgctattc cttcctattc agttaggttc
     1741 ggcaggggct gtggtcctgt ttattactgc tctggtcgcc tggcctttaa catattggcc
     1801 acataaagcc ttatgccagt tcatcctctc atcaaaaaca tcagcaggtg aagggataac
     1861 gggcgcggta acacactact atggcaagaa gattggtaat ctgattacca cgctgtactt
     1921 catcgccttt tttgtcgtcg tgttgatata tgcagtggca attaccaact cacttacgga
     1981 acagctggca aagcatatgg ttattgatct tcgcatccgt atgttggtga gtctgggtgt
     2041 tgtattaatt ctgaatctca tttttctgat gggacgtcat gccactattc gggtaatggg
     2101 atttttggta ttcccattga ttgcctattt cttatttctt tccatttacc ttgtcggtag
     2161 ttggcaacct gatctattaa caacccaggt agagttcaat cagaataccc ttcaccagat
     2221 atggatatcg attcccgtga tggttttcgc ctttagccat acgcccatta tttctacgtt
     2281 tgccatagac agacgtgaaa aatatggcga acacgctatg gataaatgca aaaaaattat
     2341 gaaagtcgct tatctcatca tctgcataag tgtactgttc tttgtcttta gctgcctgct
     2401 ttctattcca tcttcgtata ttgaagcggc taaagaagaa ggggtcacca ttttatcggc
     2461 gctttctatg ctgccgaacg ccccagcatg gttgtcaatt tccgggatta ttgtcgcagt
     2521 agttgcgatg tcgaaatcat tcctgggtac gtactttggc gttattgaag gtgccacaga
     2581 ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa agtcgtgcat ttaaccacgc
     2641 actatcaatt atgttggtat cgctgattac cttcattgtt tgttgcatta acccgaacgc
     2701 gatttcgatg atttacgcga tcagcggccc gctcattgcc atgatacttt tcatcatgcc
     2761 tacgctgtca acgtatctca tcccggcgct taaaccctgg cgttccatcg gaaatctgat
     2821 tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc tttagctaat atccgttgtg
     2881 gatggttcaa aattgctaca attttgaacc attccatttt tcataatatt catttgttat
     2941 tattccgccc tatcacttct ctcgtcatat cctagaatta tcagttagtt aaacacacaa
     3001 agagatctga acatcttaaa taagactatt taagatgcat aacttagatt cgcaagatat
     3061 cttctggtca ttcaaaaaca atttgcgata tagatattct cataccatga gatagttatc
     3121 tggaccacgt tcgaccatgc
//
LOCUS       DAZPEU010000301         3085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPEU010000301 DAZPEU010000000
VERSION     DAZPEU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.303"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..2116
                     /locus_tag="ACHU2L_004070"
     CDS             <1..2116
                     /locus_tag="ACHU2L_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358677.1"
                     /translation="SASSYAKSESPPASTITFIAQIDHVELNKTALSHDMKLVLADRF
                     YFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFFMTINQCDGNGTPM
                     LTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPLPYDEEKKQWKVTG
                     WYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIKNIANYNPKGERDG
                     KYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNNDKIKDGECPHFYED
                     GKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSYYQSGKISSIHNRN
                     SRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEKHFDEQGQLNGLVK
                     QWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDGIAKSWNKYGKLTY
                     SIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRTGKLLTSTQYVNNK
                     RHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSAQFALGKYEFICAN
                     IDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQAATLGSSNAQAEI
                     GYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDGVERNMEKARFHLS
                     NAAEGGIEPALKALNELEHQTK"
     gene            2169..>3085
                     /locus_tag="ACHU2L_004071"
     CDS             2169..>3085
                     /locus_tag="ACHU2L_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1358678.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFA"
BASE COUNT         1121 a          556 c          573 g          835 t
ORIGIN      
        1 tagcgccagt tcatatgcaa aaagcgaatc ccctcccgca agcactataa ctttcattgc
       61 acaaatcgat catgttgagc taaataaaac agcattatct catgatatga aactcgttct
      121 tgccgatcgt ttttatttca aaaccagtaa tccttgcgaa acaaattcaa tttctgctcg
      181 accagaatca atggatatta ctgacaaagc ctatgccgac aaattaaaaa aattagaaac
      241 tccttttctt gatgatcgct atttctttat gaccatcaat cagtgtgacg gtaatggcac
      301 tcccatgctg acagacataa aagtatgtac aacagaatta tgtggtgctg aatatacaaa
      361 gaaaatgtct actgtatggc tcgatgaaac tttaaaacca actgctaaac gtctggcaat
      421 ttattttatt caatctcctt taccctatga cgaggagaaa aaacaatgga aagtcaccgg
      481 atggtatatc tcatcaactg ataatcagtc ttcaactaaa cagattgcat ttgaaggata
      541 tacagacaca gataacctct ccgattctaa atttatttct tcatttaaat cctggtttga
      601 aagtggaaag ataaaaaaca ttgctaacta taatccaaaa ggagaaagag atggaaaata
      661 cgaaagttat tacaatagca atggtaacgt ctatgaatct ctaagttttt ctaatggtct
      721 tatcaacggt gagtttatta catattatga aaatggggcc attgaaagta aacgtcattt
      781 taataacgac aaaattaaag atggtgaatg ccctcatttt tatgaagatg ggaaaatcaa
      841 acagcaacat agctatctga atagtaaact cgatggtcct gcatatgaat attatcctga
      901 tggcaaactc atgcaggaaa atttctatca gcaaagtgtg ttgattggaa acgataccag
      961 ttactaccaa tccggaaaaa taagctctat acacaataga aattcccgtg gtcagtatga
     1021 tggagctaac gaacgttata gtgaagaggg taagttactg tctaaatccg tctataaaga
     1081 cggaaaacaa atttctgttc aaacctggta tgaaaatggg cagaaagaag aggagaaaca
     1141 ttttgatgag caaggccaat taaatggtct ggttaagcaa tggtataaaa atggtaatct
     1201 tgcaaaatca caaaactata aacatgacat actcgatggt aacagtgaag agtggtacga
     1261 aaatggcatc cctgaaagtt tgtaccctta taagaatgga aaaaccgacg gcattgccaa
     1321 atcatggaat aagtatggta aattaaccta ttcaatcgaa tacaaaaatg gcgtagagaa
     1381 tggggcatat cgtaactgga gcaaaaacac aggtaaacta accaaagaaa ccctgtacgt
     1441 caatggtatc agacagggag tggaaaaaga gtttaacgat cgcacaggta aactattaac
     1501 ctccacgcaa tatgttaata ataaaagaca tggtacggag gaaacttatg atcaaaacgg
     1561 aataaaatat attacatgct atcaaaatga tcagaaatta tcgtctttag acaatccaac
     1621 acaaataaaa gataatgcaa caacaggcga ttccagtgct caatttgcat tagggaaata
     1681 cgaattcatc tgtgcaaata tagatgaagg gattaaatgg ttaaccaaat ctgcagagca
     1741 aaaaaacact gatgcgatat actttttagc tacagcctac aaaggcaatg gtatacctgc
     1801 aaacaatgaa aaatacctag catatctgca acaagctgca acactgggca gtagtaatgc
     1861 ccaggcggaa attggctatt tatatctcat cggaaaagaa ctaccacaga atcttcctga
     1921 tgctggtgtc tggttcaaaa aagcagctgc acaaggtaat tttgccgcac atttctatct
     1981 gggaaggatg tatcagaatg gtgacggcgt tgagagaaat atggaaaaag ccaggtttca
     2041 tctatctaat gcggcagaag gtggaataga acctgcgctt aaagcattaa atgaacttga
     2101 acatcaaaca aaataaagcg ctcagggacc acgtaaggtt catcaaacag aaaaggatat
     2161 agtttgcgat gaaagcgaaa tccactttgt tattaatgat tttatctgtt ttttattctc
     2221 atcaggtttt atccagcgaa cccactcagc cggatgttta ttctgtcgtc gagaaaaaac
     2281 taaactctgc tttacctctg gaagaaaata ccaccttcag atcgcaggct gaatggttta
     2341 atttgcaata tgaattatta gtatctggct accctgaacg tgcttacaat ttactgagca
     2401 aacttgagac agaatcgaaa aacgctaaat attatctgga tttaactgcc tccagagtcg
     2461 ctgaagaaga agcaccagag acagccttaa cttttttatc aaaaattggg ttaaataaac
     2521 cggctactct tacatccttt gagagctata tcaatagctg gataaaaagc aaacaacccg
     2581 aaaaggctat gcagcttctc agcctcgatg agaatgccct gaattattat ttaccgactg
     2641 tgttagtcgc atatcagatc gttcctgata aggcaataag tatttataat tctgtatatg
     2701 gagataatgt tgttataccc tatcagcaag tcaccatgtt acttacagtt gcggagggtt
     2761 atcaaacaaa aggcgatata aaaaacgcca ttcattatgc cgataaggcg ctgaatatgt
     2821 ttgataaagc tattgctgta gatagcagtc gtgaatccca ttatagtgat gagtatttga
     2881 aactcatgaa tatctacgcc gccaatggca acaaagaaaa agcggtggtt ttatctcaac
     2941 gtctacacaa agccattacc gaatctcaat cgaattactt aagtactctg gaaggaattt
     3001 tattgtttta tagaaataat gatatgcagc aagactatca aaacagcctt tcaaattaca
     3061 tagtatttat cgataaaatt tttgc
//
LOCUS       DAZPEU010000302         3069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPEU010000302 DAZPEU010000000
VERSION     DAZPEU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.304"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..498
                     /locus_tag="ACHU2L_004072"
     CDS             <1..498
                     /locus_tag="ACHU2L_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1358679.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHU2L_004073"
     CDS             578..1273
                     /locus_tag="ACHU2L_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1358680.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHU2L_004074"
     CDS             1302..>3069
                     /locus_tag="ACHU2L_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1358681.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZPEU010000303         3058 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPEU010000303 DAZPEU010000000
VERSION     DAZPEU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.305"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            158..1367
                     /locus_tag="ACHU2L_004075"
                     /pseudo
     CDS             158..1367
                     /locus_tag="ACHU2L_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            complement(1371..1460)
                     /gene="yldA"
                     /locus_tag="ACHU2L_004076"
     CDS             complement(1371..1460)
                     /gene="yldA"
                     /locus_tag="ACHU2L_004076"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR1358682.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(1598..2008)
                     /gene="rnk"
                     /locus_tag="ACHU2L_004077"
     CDS             complement(1598..2008)
                     /gene="rnk"
                     /locus_tag="ACHU2L_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR1358683.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(2237..3043)
                     /gene="rna"
                     /locus_tag="ACHU2L_004078"
     CDS             complement(2237..3043)
                     /gene="rna"
                     /locus_tag="ACHU2L_004078"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR1358684.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
BASE COUNT          753 a          704 c          815 g          786 t
ORIGIN      
        1 atttgtctgg ttctttttcc ttacgaacgg tttctgtgat taatatattc tcactgaaca
       61 ctaggaattc tcccaaaacc tgcggtaccg cccgttttcc cgctgtgata gctaccctta
      121 aagactgact cttttttgaa ctgtctctgg aggttgcatg aaagcattga cttatcacgg
      181 cccacatcac gttcaggtag aaaatgttcc cgatccgggc attgaacagg cagatgatat
      241 tattctgcgt attacggtaa cggcgatctg tggctctgac ctccatcttt atcgaggcaa
      301 aataccccag gttaaacatg gcgatatttt tggtcatgaa tttatggggg aagtcgttga
      361 aaccgggaag gacgtaaaaa atttgcaaaa aggcgaccgg gtggtaattc cgttcgtcat
      421 tgcttgtggc gactgttttt tctgtcgatt acagcaatat gccgcctgcg aaaataccaa
      481 tgcgggtaaa ggcgctgcgc tcaataaaaa acagatacct gctcccgcgg cattgtttgg
      541 ttatagtcac ctgtatggtg gcgttcctgg cgggcaggcg gaatatgtcc gcgtcccgaa
      601 agggaatgtg gggccgttta aagtaccgcc tttgctttca gatgataaag cccttttcct
      661 ttctgatatt ctgccaacgg catggcaggc agcaaaaaat gtgcagatcc aacaaggttc
      721 aagcgttgca gtctatggtg ctggtcctgt gggattgttg acaatcgcct gtgcacggtt
      781 gctcggtgcg gaacagattt ttgttgttga tcatcatccc taccgcttgc gtttcgccgc
      841 tgaccgctac ggcgcgatcc cgattaattt cgatgaagat agcgatccgg cacagtcaat
      901 tattgaacaa acggcaggtc accggggcgt ggatgcagta atagacgccg tcggttttga
      961 agcgaaaggc agcaccacgg aaacggtgct gactaacctg aaactggagg gcagcagcgg
     1021 taaagcgttg cggcattgtt agcgtaccgg gcgtctacgc tggatttatt cacggtttcc
     1081 tgtttggcga cgcctttgat aaagggttga cgtttaaaat gggacagacc cacgttcacg
     1141 catggctggg agaattatta ccgttaattg agaaaggatt actgaaacca gaagaaattg
     1201 ttacccacta tatgccgttt gaagaggccg cccggggata tgagattttc gaaaaacgtg
     1261 aagaggagtg ccgtaaggtg attctggtac ccggtgcaca aagcgcagag gcggcgcaga
     1321 aggcggtttc aggtctggtg aatgcgatgc cggggggaac aatatgatcg tcaggagtgg
     1381 ttttcgaggt aaaggacagc catgacgata atcgccgcca taatcagaaa tcctatcagg
     1441 atgtaaaatg cttctgccat ggttattccc acaaacgaaa cggaataatt ttgcagcaaa
     1501 agtgaacagt gagaaccagg aaaaattgct gattttgcgt aaagaggatg cgagtgcatc
     1561 ctctgggcaa agcgagttat cgcttgtgca gatgggatta aagcaggtag tcgccagcag
     1621 cttctggctg gtattcgagt tccagcactt caaggtgggt tgcaacgccg cccggaagtt
     1681 cccagtgaat ggaatcgcca acgcgcagcc ccagcagtgc ggcacctacc ggagccataa
     1741 cggaaagctg agtattgcta tcggtcattt ttgccggata caccagcgtg cgcacacgca
     1801 cttcgccatc gctaagattg cggaatttaa cccggctgtt cattgtcacc acgtcgtgtg
     1861 gcatctcttc tggcgaacac atttgggcgc gatccaactc tgcgtttaac gcgtcggcga
     1921 ttggcaaacc agcataggcg ggttgctcca gcagaatatc gatgcgttcg gcatccaggt
     1981 cgttaatgat gatagttggt ctggacattt ttactccatg tcgtcggtac tgcgagtgtc
     2041 gcagataaac atacccaaaa gaaaaccctc accgtcaggc ggcgagggtt taactcacat
     2101 gatgatactg actgttgctc actctttgaa gtgatttgcg tcacattcag ggaattcctc
     2161 aatgcagcgc attatgtata attcttaatc ggcatggtta tggaagacga attagcgtgt
     2221 tttgtaaatc agaggattaa taacccgctt tatcaatcac aaaggttttg ccacagttac
     2281 ctgggtgagg ttgtggcaag aatgagtttg cagaaagcgg agcgttgatg gcgtcagctt
     2341 tgatcgagat ctgaatttca gtcaaatacg cagggttacc ctggcacgtt agcttaactg
     2401 ctttcacgtt ctcttttccc cagcttttgg caaaggcggc gtcaaagtca cggcggctca
     2461 ctgttttacc gtaattatcc gcaaggaatt ttccggcttc gctctcttta atttcttgat
     2521 tcaggcgtac catcgtaccg aagtatgcgt ccggatcaaa accaaagcag gcaccgtgtt
     2581 tggcatattc gtagcgttcc aggcaggaac gtccgccagc tcctggcatg acttcactta
     2641 gtttagcggc cgtttccagt gataacccgg tttccggcga tgaacacatt cggctggcgc
     2701 gtgcttctgg tagattcggg attgggcgag tagcgcaacc gaagcgcatc cagcggcgtt
     2761 catcaacacc acgggcagca accgatttag gcaatcctgg ccacagacca tgtacggtca
     2821 gaaaatcagt tttgttggtc gtttcggttt gcaggcgaca ttcatctcgt tcgttacgat
     2881 ttcgatcgtg ttgactctgg caaaatccgg tttgccagga gagggccagg acatagcgat
     2941 caaaatcgcc atactgtttt gcctgcaacg ctacggcgtt ggcagaagat aagggaagca
     3001 gagaaaccgc gagcaacgcg gcgttacggc agaatgcttt cataatgggt gtggaact
//
LOCUS       DAZPEU010000304         3033 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPEU010000304 DAZPEU010000000
VERSION     DAZPEU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.306"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..2853
                     /locus_tag="ACHU2L_004079"
     rRNA            <1..2853
                     /locus_tag="ACHU2L_004079"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2949..>3033
                     /gene="rrf"
                     /locus_tag="ACHU2L_004080"
     rRNA            2949..>3033
                     /gene="rrf"
                     /locus_tag="ACHU2L_004080"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          801 a          667 c          947 g          618 t
ORIGIN      
        1 aaggacgtgc taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga
       61 tttccgaatg gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt
      121 aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga
      181 gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg
      241 ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc
      301 acatactgtg agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg
      361 accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa
      421 aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc
      481 agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat
      541 attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg
      601 ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg
      661 ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg
      721 ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg
      781 tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg
      841 acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc
      901 gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag
      961 tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag
     1021 cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg
     1081 taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg
     1141 ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta tcagaagtgc
     1201 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
     1261 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
     1321 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
     1381 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
     1441 gcaaatccgg aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc tgaagcaaca
     1501 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
     1561 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
     1621 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagcgacttg
     1681 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
     1741 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
     1801 taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
     1861 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
     1921 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
     1981 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
     2041 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
     2101 gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg
     2161 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
     2221 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
     2281 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
     2341 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     2401 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     2461 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     2521 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
     2581 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
     2641 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
     2701 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
     2761 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
     2821 aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagatgtt ttggcggatt
     2881 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     2941 agaattagcc tggcggccgt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     3001 tgaaacgccg tagcgccgat ggtagtgtgg ggt
//
LOCUS       DAZPEU010000305         2997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPEU010000305 DAZPEU010000000
VERSION     DAZPEU010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.307"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACHU2L_004081"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACHU2L_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1358685.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHU2L_004082"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHU2L_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR1358686.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHU2L_004083"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHU2L_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR1358687.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..>2997
                     /locus_tag="ACHU2L_004084"
     CDS             1993..>2997
                     /locus_tag="ACHU2L_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358688.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     K"
BASE COUNT          822 a          638 c          714 g          823 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
     2281 tattcagggg tttcaggagg tattacatat actatacaat atgttcaaga tgttgatatt
     2341 gttagagtgt ccttgccggg cagaggttca gagtctatca cagattttaa aggttattat
     2401 tggtatggct ttatggaata tattgaaaac attaatgcgt gtgatgatgt tttttctgag
     2461 tattgtttag ataatgaaaa tatgagtatc cagccagaac agataaatat gccgggaata
     2521 tctgatttag atactggtat tgatttgtct ggcatatctt ttattcagag tgaaattaat
     2581 aaaacatatg gattaaaata cgctccagta gatggcgatg gatattgtct gttaagagcc
     2641 atactggttt taaaagaaca tgagtattca tgggcgctgg gcagccataa gacgcaaaag
     2701 caagtatacg aagagtttat taaaattgtt gataaacaaa cgatcgaggc tcttgttgat
     2761 acggcattct ataatctcag ggaagatgta aagacattat ttggcgttga tctgcaatct
     2821 gacaacaaaa ttcagggtgt gggtagtttt atgtcatgga gctttctgtt ttttaaaaaa
     2881 caattcattg atagttgctt gaatgacaaa aaatgcatac tgcatttacc cgagtttata
     2941 tttaatgata acaagaattt gcttgcttta gataccgaca cgtcggatag gattaaa
//
LOCUS       DAZPEU010000306         2994 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPEU010000306 DAZPEU010000000
VERSION     DAZPEU010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.308"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHU2L_004085"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHU2L_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1358689.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHU2L_004086"
     CDS             348..641
                     /locus_tag="ACHU2L_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR1358690.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHU2L_004087"
     CDS             713..1372
                     /locus_tag="ACHU2L_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR1358691.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHU2L_004088"
     CDS             1464..1910
                     /locus_tag="ACHU2L_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR1358692.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..2994)
                     /gene="hlyE"
                     /locus_tag="ACHU2L_004089"
                     /pseudo
     CDS             complement(2117..2994)
                     /gene="hlyE"
                     /locus_tag="ACHU2L_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT          781 a          646 c          718 g          849 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgtt
//
LOCUS       DAZPEU010000307         2951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPEU010000307 DAZPEU010000000
VERSION     DAZPEU010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.311"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHU2L_004090"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHU2L_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR1358693.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHU2L_004091"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHU2L_004091"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR1358694.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACHU2L_004092"
     CDS             2603..>2951
                     /locus_tag="ACHU2L_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1358695.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZPEU010000308         2936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPEU010000308 DAZPEU010000000
VERSION     DAZPEU010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.312"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..533)
                     /gene="syd"
                     /locus_tag="ACHU2L_004093"
     CDS             complement(<1..533)
                     /gene="syd"
                     /locus_tag="ACHU2L_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR1358696.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQL"
     gene            601..1449
                     /gene="queF"
                     /locus_tag="ACHU2L_004094"
     CDS             601..1449
                     /gene="queF"
                     /locus_tag="ACHU2L_004094"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR1358697.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            1561..2925
                     /gene="ppnN"
                     /locus_tag="ACHU2L_004095"
     CDS             1561..2925
                     /gene="ppnN"
                     /locus_tag="ACHU2L_004095"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR1358698.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT          718 a          731 c          720 g          767 t
ORIGIN      
        1 ttaagttgat taaggaattc cgcaagattt gaggctaaat tcgtccgttt acgtgttccc
       61 agcgtctctt tgcacacttc accggagaga ttacacaccg agatgacctc taactcttct
      121 tccagcgtcg cgataaatag cgtgggcggc agttttagcc ttttctgagt caccagatga
      181 ccaatcagat tttcctgcac ccgacggaag tcgtcttcac tccaggtttg cagcaatgtc
      241 agtttgatat cgccaaactg cgcgtgcata tccccggcaa actgagtcgt ataaaaggta
      301 tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa ccgcgtttac attttgttcc
      361 ccggtaaacg gctggggttg ccagtatacg gcatcttcgg tagttgaaat aatgcacggt
      421 gaaggaacac cgtacaattc ctcacttaac ggccagcttt tatgctcttc atgccacgca
      481 tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg tcaaatcgtc cactaatttc
      541 tctcttcact taaaccagat acacttgtcg tttagtttat ctggtttatg acggtgaaac
      601 atgtcttctt atgcaaacca tcaggcactt gcgggcctga ctcttggaaa atcaaccgat
      661 taccgggata cctatgacgc cagcctactg caaggcgttc cacgcagcct gaatcgcgac
      721 ccgctgggtc tgaaagcgga taacctgcct tttcacggta cggatatctg gacgctgtat
      781 gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg ctgtcggtca tgttgaactt
      841 gattacacca gcgtaaatct gattgagtcg aagagtttta agctctatct caacagtttt
      901 aaccagacgc gttttaataa ctgggatgag gtgcgccaga cgctggagcg cgacttaagc
      961 acttgcgctc agggtaagat tagcgtggcg ttatatcgtc ttgatgaact ggaaggccag
     1021 ccgataggtc atttcaatgg cacttgcatt gatgaccagg atatcactat cgataactat
     1081 gaattcacta ctgactatct ggagaatgcc accagcggtg aaaaagtagt ggaagagacg
     1141 ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc atcaaccaga ttggggttcg
     1201 atccaaattc agtatcgtgg acgccaaatt gacagagaaa aactgctgcg ttacctggtc
     1261 tcattccgtc atcacaacga gttccacgaa cagtgcgtgg aacgcatctt taatgacctg
     1321 ttacgcttct gccagccaga gaaattgagc gtttacgcac gttatacccg tcgtggcggt
     1381 ctggacatta acccgtggcg cagtaatagc gattttgtcc catcgaccac aagactggtt
     1441 cggcaataaa ttttttctca attttgcgtg ctggattcac gcagaaggtt gtgaaaggtc
     1501 atcaggcagg gctattgtaa tcaaagggaa tgacgatatt cgtcccataa ggagtttttc
     1561 ttgattacac atattagccc gcttggctcc atggatatgt tgtcgcagct ggaagtggat
     1621 atgcttaaac gcaccgccag cagcgacctc tatcaactgt ttcgcaactg ttcacttgcc
     1681 gtactgaact ccggtagttt gaccgataac agcaaagaat tgctgtctcg ttttgaaaat
     1741 ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc tggaactgat taatcccccg
     1801 gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc aggccaactt gttcgcggtt
     1861 ctgcgagaca ttctcttcgt ttacgggcaa atccatcata ccgttcgttt tcccaacctg
     1921 aatctcgaca actccgtcca catcactaac ctggtctttt ccatcttgcg taacgctcgc
     1981 gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct ggggcggtca ctcaattaac
     2041 gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc tgggcctgcg tgagctgaat
     2101 atctgcaccg gctgtggtcc aggagcgatg gaagcgccga tgaaaggtgc tgcggtcgga
     2161 cacgcgcagc agcgttacaa agacagtcgt tttattggta tgacagagcc gtcgattatc
     2221 gccgctgaac cgcctaaccc actggtcaac gaattgatca tcatgccgga tatcgaaaaa
     2281 cgtctggaag cgtttgtccg tatcgctcac ggcatcatta tcttccctgg cggtgtgggt
     2341 acggcagaag agttgctgta tttgctggga attttaatga acccggccaa caaagatcag
     2401 gttttaccat tgatcctcac cggcccgaaa gagagcgccg actacttccg cgtactggac
     2461 gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc attaccgcat catcattgat
     2521 gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc cgctggtgaa agaaaatcgc
     2581 cgtgatacag gcgatgccta cagctttaac tggtcaatgc gcattgcgcc agatttgcaa
     2641 atgccgtttg agccgtctca cgagaatatg gctaatctga agctttaccc ggatcaacct
     2701 gttgaagtgc tggctgccga cctgcgccgt gcgttctccg gtattgtggc gggtaacgta
     2761 aaagaagtcg gtattcgcgc cattgaagag tttggtcctt acaaaatcaa cggcgataaa
     2821 gagattatgc gtcgtatgga cgacctgcta cagggttttg ttgcccagca tcgtatgaag
     2881 ttgccaggct cagcctacat cccttgctac gaaatctgca cgtaatctcc cctccc
//
LOCUS       DAZPEU010000309         2932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPEU010000309 DAZPEU010000000
VERSION     DAZPEU010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.313"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHU2L_004096"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHU2L_004096"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR1358699.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHU2L_004097"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHU2L_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR1358700.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHU2L_004098"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHU2L_004098"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR1358701.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACHU2L_004099"
     CDS             2756..>2932
                     /locus_tag="ACHU2L_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358702.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZPEU010000310         2921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPEU010000310 DAZPEU010000000
VERSION     DAZPEU010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.314"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..534
                     /locus_tag="ACHU2L_004100"
     CDS             <1..534
                     /locus_tag="ACHU2L_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFR1358703.1"
                     /translation="SARILFVEKGYQAVSIDEISGKALVTKGAFYHHFKNKKQLLSAC
                     YKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNKNLIPIQEVMPIIGWNEL
                     EKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKK
                     GQFIAIYRGFLLSLKDK"
     gene            complement(647..1852)
                     /gene="tet(B)"
                     /locus_tag="ACHU2L_004101"
     CDS             complement(647..1852)
                     /gene="tet(B)"
                     /locus_tag="ACHU2L_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR1358704.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            1934..2557
                     /gene="tetR(B)"
                     /locus_tag="ACHU2L_004102"
     CDS             1934..2557
                     /gene="tetR(B)"
                     /locus_tag="ACHU2L_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR1358705.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2535..>2921)
                     /locus_tag="ACHU2L_004103"
     CDS             complement(2535..>2921)
                     /locus_tag="ACHU2L_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1358706.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
BASE COUNT          991 a          605 c          558 g          767 t
ORIGIN      
        1 tcagcacgaa tattgtttgt tgaaaaaggc tatcaagctg tttcaataga tgagatctcg
       61 ggaaaagcgt tggtgaccaa aggtgccttt tatcatcact ttaaaaataa aaaacaatta
      121 ctcagtgcct gttataagca gcaattaatt atgattgatg cctacatcac aacaaaaact
      181 gatttaacaa atggttggtc tgccttagaa agtatatttg aacattatct tgattatatt
      241 attgataata ataaaaacct tatccctatc caagaagtga tgcctatcat tggttggaat
      301 gaacttgaaa aaattagcct tgaatacatt actggtaagg taaacgccat tgtcagcaaa
      361 ttgatccaag agaaccaact taaagcttat gatgatgatg tgcttaaaaa cttactcaat
      421 ggctggttta tgcatatcgc aatacatgcg aaaaacctaa aagagcttgc cgataaaaaa
      481 ggccaattta ttgctattta ccgcggcttt ttattgagct tgaaagataa ataaaataga
      541 taggttttat ttgaagctaa atcttcttta tcgtaaaaaa tgccctcttg ggttatcaag
      601 agggtcatta tatttcgcgg aataacatca tttggtgacg aaataactaa gcacttgtct
      661 cctgtttact cccctgagct tgaggggtta acatgaaggt catcgatagc aggataataa
      721 tacagtaaaa cgctaaacca ataatccaaa tccagccatc ccaaattggt agtgaatgat
      781 tataaataac agcaaacagt aatgggccaa taacaccggt tgcattggta aggctcacca
      841 ataatccctg taaagcacct tgctgatgac tctttgtttg gatagacatc actccctgta
      901 atgcaggtaa agcgatccca ccaccagcca ataaaattaa aacagggaaa actaaccaac
      961 cttcagatat aaacgctaaa aaggcaaatg cactactatc tgcaataaat ccgagcagta
     1021 ctgccgtttt ttcgccccat ttagtggcta ttcttcctgc cacaaaggct tggaatactg
     1081 agtgtaaaag accaagaccc gctaatgaaa agccaaccat catgctattc catccaaaac
     1141 gattttcggt aaatagcacc cacaccgttg cgggaatttg gcctatcaat tgcgctgaaa
     1201 aataaataat caacaaaatg ggcatcgttt taaataaagt gatgtatacc gaattcgatt
     1261 gcgtctcaac ccctacttcg gtatctgtat tatcacgtgt atttttggtt tcacggaacc
     1321 aaaacataac cacaaggaaa gcgacaatat ttagcaacgc agcgataaaa aagggactat
     1381 gcggtgaaat ctctcctgca aaaccaccaa taataggccc cgctattaaa ccaagcccaa
     1441 aacttgcccc taaccaaccg aaccacttca cgcgttgaga agctgaggtg gtatcggcaa
     1501 tgaccgatgc cgcgacagcc ccagtagctc ctgtgatccc tgaaagcaaa cggcctaaat
     1561 acagcatcca aagcgcactt gaaaaagcca gcaataagta atccagcgat gcgcctatta
     1621 atgacaacaa cagcactggg cgccgaccaa atcggtcaga catttttcca agccaaggag
     1681 caaagataac ctgcattaac gcataaagtg caagcaatac gccaaagtgg ttagcgatat
     1741 cttccgaagc aataaattca cgtaataacg ttggcaagac tggcatgata aggccaatcc
     1801 ccatggcatc gagtaacgta attaccaatg cgatctttgt cgaactattc atttcacttt
     1861 tctctatcac tgatagggag tggtaaaata actctatcaa tgatagagtg tcaacaaaaa
     1921 ttaggaatta atgatgtcta gattagataa aagtaaagtg attaacagcg cattagagct
     1981 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt
     2041 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc
     2101 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca
     2161 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg
     2221 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca
     2281 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc
     2341 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga
     2401 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt
     2461 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg
     2521 attagaaaaa caacttaaat gtgaaagtgg gtcttaaaag cagcataacc tttttccgtg
     2581 atggtaactt cacggtaacc aagatgtcga gttaaccacc ctttagattc ataaagcgaa
     2641 aataatgcgg ctccaacgta cccacctaaa tggaaacggc gttcactcca atctaaacac
     2701 gcacaacaga ttttacgtga atgtttggaa ggaacgtcaa ttcccatttc atgaaaatat
     2761 tgaataccac ttaatgtgat cattgaacca ttttcagtga tccattgctg ttgacaaagg
     2821 gaatcataga tcttaacggc aacttcgcca gctaaatgat catagcaagt acgtgctttt
     2881 cgtaaatgca ctggcgtgga aactttggca tgtacgccat g
//
LOCUS       DAZPEU010000311         2898 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPEU010000311 DAZPEU010000000
VERSION     DAZPEU010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.315"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHU2L_004104"
     CDS             complement(<1..312)
                     /locus_tag="ACHU2L_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1358707.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DI"
     gene            complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACHU2L_004105"
                     /gene_synonym="yehT"
     CDS             complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACHU2L_004105"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR1358708.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACHU2L_004106"
     CDS             complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACHU2L_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR1358709.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          750 a          774 c          658 g          716 t
ORIGIN      
        1 aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt
       61 aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat
      121 caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc
      181 ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattat ccagcgccag
      241 aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc
      301 gttacttatc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
      361 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
      421 cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg
      481 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccaaggtac
      541 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
      601 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
      661 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
      721 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
      781 gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga
      841 gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga
      901 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
      961 tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa
     1021 atatacgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
     1081 ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
     1141 gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
     1201 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
     1261 caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga
     1321 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
     1381 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
     1441 ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga
     1501 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
     1561 ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg
     1621 attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc
     1681 atattgccct gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
     1741 gtttattgaa ctgaataacc ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg
     1801 ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
     1861 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
     1921 ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
     1981 ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg
     2041 atacagtacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc
     2101 ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
     2161 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
     2221 catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
     2281 cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat
     2341 ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc
     2401 gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga
     2461 atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa
     2521 gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc
     2581 aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg
     2641 cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc
     2701 aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat
     2761 acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct
     2821 ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa
     2881 aataaaatca ccctatag
//
LOCUS       DAZPEU010000312         2889 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPEU010000312 DAZPEU010000000
VERSION     DAZPEU010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.316"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACHU2L_004107"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACHU2L_004107"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR1358710.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACHU2L_004108"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACHU2L_004108"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR1358711.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACHU2L_004109"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACHU2L_004109"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR1358712.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="ACHU2L_004110"
     CDS             complement(1895..2809)
                     /locus_tag="ACHU2L_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR1358713.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          771 g          691 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattacct
//
LOCUS       DAZPEU010000313         2862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPEU010000313 DAZPEU010000000
VERSION     DAZPEU010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.317"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHU2L_004111"
     CDS             complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHU2L_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR1358714.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHU2L_004112"
     CDS             complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHU2L_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR1358715.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHU2L_004113"
     CDS             complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHU2L_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR1358716.1"
                     /translation="SANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAAS
                     RITSLFLGLKIISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFG
                     FHGNIPSLIICYGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGG
                     NIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGR
                     FKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFT
                     VWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          833 a          745 c          727 g          557 t
ORIGIN      
        1 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
       61 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      121 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      181 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      241 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      301 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      361 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      421 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      481 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      541 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      601 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      661 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      721 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
      781 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
      841 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
      901 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
      961 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1021 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1081 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1141 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1201 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1261 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1321 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1381 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1441 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1501 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1561 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1621 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1681 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1741 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     1801 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     1861 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     1921 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     1981 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2041 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2101 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2161 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2221 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2281 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2341 tgtttggtgc cgaggaacga tttcaccagc gaatcaatgt tgccgcctga ggagattatc
     2401 gccttaaagc tttcgcgcgg aatattcccc atcgtgcaat agagccagaa gagataaatc
     2461 accagcgcca gcagcgaacc aaataccacg cttttgatta acttatcttt gcgtttgcca
     2521 tagcaaataa tcaggctggg aatattgccg tggaaaccaa atgacgccag acacaccggc
     2581 aaagccataa agatatacgg gaagtaagac gttcccgcag tggtgctggt ggcatcgcgc
     2641 aggatggagt aatcgacctg gaagaagaaa gaaccaaaca cgatcacaaa ggagataatc
     2701 ttcagcccga ggaacaatga ggtaatgcga ctggcggcta acgaacttat ccacaatacg
     2761 ctggcaacga aaatggctgt gcagatcccg acaatacgtg gattagcgtg atagcccaaa
     2821 ttcattgata tcgtttcact aatgatcgca ccattagcag ag
//
LOCUS       DAZPEU010000314         2855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPEU010000314 DAZPEU010000000
VERSION     DAZPEU010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.318"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..839)
                     /locus_tag="ACHU2L_004114"
     CDS             complement(<1..839)
                     /locus_tag="ACHU2L_004114"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HFR1358717.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKL"
     gene            complement(1011..1649)
                     /gene="purN"
                     /locus_tag="ACHU2L_004115"
     CDS             complement(1011..1649)
                     /gene="purN"
                     /locus_tag="ACHU2L_004115"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR1358718.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(1649..2686)
                     /gene="purM"
                     /locus_tag="ACHU2L_004116"
     CDS             complement(1649..2686)
                     /gene="purM"
                     /locus_tag="ACHU2L_004116"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR1358719.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
BASE COUNT          699 a          757 c          706 g          693 t
ORIGIN      
        1 agtttttcgc gtaacacttc aaccagcgca ttgggcatat cgcgctgata aacaaaacgc
       61 accggctcag cagttaaacg ctgcttgaga ctggaagaca tcaactccat caggctggct
      121 tccatctcat gcactaaatc gtattcggca tcgcgggtca tcttcattga ataggcattc
      181 agcgcgtcat aatcaaagaa gcctttgaaa atatcatcaa ggcagtaacg cagaatgtta
      241 tccagaagaa tcatcggctt gcgtcgacgc ggcgcttctg gcggtaaatt cacaaagcgc
      301 ggcactttat ctgatgggat ctccagcagc gcgtaacgga tgctatcgct acggataatt
      361 tccaccgcca gataggtgta atcatctttc aggaactgca ctaagtcagt gtcaggattg
      421 attaaaatcg gcgtaatgtg ctgacgcaga tactgcttaa aataatgacg cagccagttt
      481 tgttgattga cggagagctg gcgttcatta atcaggaaga tctggttgcg cgccatctcc
      541 agcaatagct cgttgtagag gccgtcgaat tcctgatcgg ctttcagcac ccgggactga
      601 attttgccca gtaaatggcg ggaatgagcg ttggagcctt gttcttcgct aataatgatg
      661 cgtcgcttca gttcagcgaa gcggacttta tagaactcat caaggttatt ggaatagatc
      721 cccaggaaac gcatcctttc aatcagcggg ttagatttgt ccgccgcttc ctgaagcacg
      781 cgttcattga acgataacca actgagctct ttttcgatgt atagcttttc ctgacccatt
      841 accactttta ctccgtttta ttcacgggac actgcctgga tattatggcg agcaatattg
      901 gcagatgtcc aactatgcca gaaaaggggg aaaaaaactg ctggagcttg cgatttacag
      961 gtgaattgaa aaacgccagc ggcagagctg gcgctttaac tacgggggca ttactcgtcg
     1021 gcagcgtagc cctgcggcgg cagacgttga ccatccagcc acgcggcgtt ttcgtgcatt
     1081 ttcagacgac catcggcaaa ccagctaatc accagtggat aaatagcgtg ttcctgggtt
     1141 tgcacgcggg cggtgatgtc atcttccgta tcaccagcaa ataccgggac tttcgcctgt
     1201 aaaataaccg ggccaccgtc cagttcatcg gtgacgaaat gcaccgatgt accgtgctct
     1261 tcatcgccat tttccagcgc ctgacgatgg gtgtgtaatc cgggatattt cggcagcaga
     1321 gaagggtgaa tgttcagcaa acgcccggca tagtgggaga caaacgccgg gctgagaatg
     1381 cgcataaaac cagccagcac gaccacatcg ggtgcgtaca tgtcgatttc atgaatcaac
     1441 tcccggtcat aggcttcacg actgtcaaac gcgctggcga tgagcgtatg cgttgcaata
     1501 cccgcctggc gggcgcgttc aaggccgaac gcgtcggcct tattgctgaa aactgcccgt
     1561 acggtgcctt taattttgtt ggttttacag gcgtcaataa ttgcctgtaa attacttccg
     1621 ttgccggaaa taagcaccac aatattcatt attcgataac cacgcgttgt tcggaatcag
     1681 aggctttgat gataccgatt ttccacgcgt tttcaccgtt ggcatttagc agggcgaggg
     1741 ctttgtccac ttccggagca ggcagggcga taatcatccc gacgccgcag ttgaaggtgc
     1801 gatacatttc atggcgctca acgttacctg ccgtttgcag ccagttgaac acttccggcc
     1861 actgccagga agattcatca atcactgcct gggtattatc tggcaatacg cgcggaatgt
     1921 tttcccagaa gccgccgccg gtcaggtgcg caatggcatg cacatcgacc ttttcaatca
     1981 actccagcac tgacttcacg taaatgcggg tcggtgccag cagatgatcg gctaatggct
     2041 taccatcaag ttcggtggtt tgcggatcac aaccgctgac ttcaagaatt ttgcgcacca
     2101 gcgaatagcc gttcgagtgc ggaccgctgg aaccgagtgc aatcagcaca tcgccgtcgc
     2161 tgactttaga gccgtcgatg atttctgatt tttctaccac gcccacgcag aaacccgcga
     2221 catcgtaatc ttcaccgtga tacatccccg gcatttctgc cgtttcgcca cccaccagag
     2281 aacagcccga ttgcagacaa ccttccgcga tgccgctgat caccgctgaa gcggtatcaa
     2341 catccagttt tccggttgcg taatagtcga ggaaaaacag cggttccgca ccttgcacca
     2401 ccaggtcatt aacgcacatg gcgaccagat caataccaat ggtgtcgtga cgttttaagt
     2461 ccattgccag acgcagcttg gtacctacgc cgtcagtgcc ggaaaccagc acgggttcac
     2521 gatatttttg cggcaatgca cacagcgcac cgaagccgcc cagaccgccc atcacttccg
     2581 gacgacgcgt tttcttcact acgcctttga ttcttccaac cagagcatta cccgcgtcaa
     2641 tatcaacacc ggcatctttg tagctaagag aggttttatc ggtcactgct tgggtcccca
     2701 cgcgttactt gcggtagaaa aataaaattc ggcgcaattc taacagggaa agcaaacgtt
     2761 tgcgagactg ttttacacaa cctttttgca cgtcttttcc ccgggcgcgc ggcgaaagaa
     2821 gacttgtgcc agggcaaagg ttagttttcg gatgg
//
LOCUS       DAZPEU010000315         2854 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPEU010000315 DAZPEU010000000
VERSION     DAZPEU010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.319"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..176
                     /locus_tag="ACHU2L_004117"
     CDS             <1..176
                     /locus_tag="ACHU2L_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFR1358720.1"
                     /translation="NIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLG
                     AKMQALRIFLSRK"
     gene            451..1647
                     /gene="kbl"
                     /locus_tag="ACHU2L_004118"
     CDS             451..1647
                     /gene="kbl"
                     /locus_tag="ACHU2L_004118"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR1358721.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            1657..2682
                     /gene="tdh"
                     /locus_tag="ACHU2L_004119"
     CDS             1657..2682
                     /gene="tdh"
                     /locus_tag="ACHU2L_004119"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR1358722.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          661 a          655 c          782 g          756 t
ORIGIN      
        1 ataacattgt ggacgatgac cagggcattt ttgtcatgtg ctattacaaa cgttccgatc
       61 tcttcaacct aaactatctg gggagaggaa aatggttcga tttgtttcgc tgcttcagga
      121 gtaacacttt aggggcaaaa atgcaggcgc tgagaatttt cctttcaaga aaataggttt
      181 ccctccatta gtgggtagcg ttttgatgcc aactgagata ggaaaaatga tgctttggca
      241 tcattttttc tttttatatc aatgcagtaa aatcactcat cattatgctc agccaaaact
      301 gtacagtttt tctactatct gtaggtgttg agagacatca agatacacgt tatttatcct
      361 gaattttgca gaagtgttaa cgcatcatct cgtcgcgacc tataagtttg ggtaatatgt
      421 gctggaattt gccctgtctg gagaatcgca atgcgtggag aattttatca gcagttaacc
      481 aacgatctgg aaaccgcacg ggcggaaggg ttgtttaaag aagagcgcat tattacgtct
      541 gcgcagcaag cagatatcac cgtggctgat ggaagccacg tcattaactt ttgtgccaac
      601 aactatctcg ggctggcgaa tcatccggat ctgattgcgg cagcaaaggc gggaatggat
      661 tctcacggtt tcggcatggc ttcggtgcgt tttatttgcg gcactcagga cagccataaa
      721 gagcttgaac aaaaactggc ggcattcctg gggatggaag atgcgattct ctactcttcc
      781 tgctttgatg ctaacggtgg cctgtttgaa acgctgctgg gcgcggaaga cgccattatc
      841 tccgacgcac tgaaccacgc gtctattatt gatggtgtgc gtctgtgtaa agctaaacgc
      901 tatcgctatg ccaacaacga tatgcaggag cttgaagctc gtctgaaaga agcgcgtgaa
      961 gccggtgcgc gtcatgtgct gattgccacc gatggtgtgt tctcgatgga cggcgtgatt
     1021 gccaatctga agggcgtttg cgatctggca gataaatacg atgccctggt gatggtagac
     1081 gactcccacg cggtcggttt tgtcggtgaa aatggtcgtg gttcccatga atactgcgat
     1141 gtgatggggc gtgtcgacat catcaccggc acgctcggta aagcgctggg cggggcttct
     1201 ggtggttata ccgcggcgcg caaagaagtg gttgagtggc tgcgccagcg ttctcgtccg
     1261 tacctgttct ccaactcgct ggcaccagca attgtcgccg cttccatcaa agtgctggag
     1321 atggttgaag cgggtagcga gttgcgtgac cgtctgtggg cgaacgcgcg tcagttccgt
     1381 gagcaaatgt cggcggcggg ctttaccctg gcgggagccg atcacgccat tattccggtc
     1441 atgcttggtg atgcggtagt ggcgcagaaa tttgcccgag agctgcaaaa agagggcatt
     1501 tacgtcaccg gtttcttcta tccggtcgtt ccgaaaggtc aggcgcgtat tcgtacccag
     1561 atgtctgcgg cgcatacccc tgagcaaatt acgcgtgcag tagaagcatt cacgcgtatt
     1621 ggtaaacaac tgggcgttat cgcctgagga tgtgagatga aagcgttatc caaactgaaa
     1681 gcggaagagg gcatctggat gaccgacgtc cctgtaccgg aactcgggca taacgatctg
     1741 ctgattaaaa tccgtaaaac agccatctgc gggactgacg ttcacatcta taactgggat
     1801 gagtggtcgc aaaaaaccat cccggtgccg atggtcgtgg gccatgaata tgtcggtgaa
     1861 gtggtaggta ttggtcagga agtgaaaggc ttcaaaatcg gcgatcgcgt ttctggcgaa
     1921 ggccatatca cctgcggtca ctgtcgcaac tgtcgtggcg gtcgtaccca tctgtgccgc
     1981 aacaccattg gtgtcggtgt taaccgtccg ggctgcttcg ccgaatatct ggtgattccg
     2041 gcgttcaacg ctttcaaaat ccccgacaat atctccgacg acctggcttc catttttgat
     2101 cccttcggta acgccgtgca taccgcgctg tcgttcgatc tggtgggcga agatgtgctg
     2161 gtttctggtg caggtccgat aggtattatg gctgcggcgg tggcgaaaca cgttggtgca
     2221 cgcaatgtgg tgatcaccga tgttaacgaa taccgccttg agctggcgcg caaaatgggt
     2281 atcacccgtg cggttaacgt cgcgaaagaa aaccttaatg atgtgatggc tgaactgggc
     2341 atgaccgaag gctttgatgt cggtctggaa atgtccggcg cgccgccagc gtttcgtacc
     2401 atgctcgata ccatgaacca cggcggtcgt attgcgatgc tgggtattcc accgtctgat
     2461 atgtctatcg actggaccaa agtgatcttt aaaggcttgt tcattaaagg catttacggt
     2521 cgtgagatgt ttgaaacctg gtacaagatg gcggcgctga ttcagtctgg cctcgatctc
     2581 tcgccgatca ttacccatcg tttctctatc gatgatttcc agaagggctt tgacgctatg
     2641 cgttcgggcc agtccgggaa agttattctg agctgggatt aacacgaaca agggctggta
     2701 ttccagccct tttatctgag gataatctgt taattatgta aaatcctgtc agtgtaataa
     2761 agagttcgta attgcgctga tcttttatat agctgttctc attatctctc taccctgaag
     2821 tgactctctc acctgtaaaa ataatatctc acag
//
LOCUS       DAZPEU010000316         2807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPEU010000316 DAZPEU010000000
VERSION     DAZPEU010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.320"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHU2L_004120"
     tRNA            6..81
                     /locus_tag="ACHU2L_004120"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHU2L_004121"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHU2L_004121"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHU2L_004122"
     CDS             complement(299..1057)
                     /locus_tag="ACHU2L_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR1358723.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHU2L_004123"
     CDS             complement(1065..2168)
                     /locus_tag="ACHU2L_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR1358724.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACHU2L_004124"
     CDS             complement(2178..>2807)
                     /locus_tag="ACHU2L_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR1358725.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAZPEU010000317         2807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPEU010000317 DAZPEU010000000
VERSION     DAZPEU010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.321"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            47..679
                     /gene="comR"
                     /locus_tag="ACHU2L_004125"
     CDS             47..679
                     /gene="comR"
                     /locus_tag="ACHU2L_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR1358726.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(741..1280)
                     /locus_tag="ACHU2L_004126"
     CDS             complement(741..1280)
                     /locus_tag="ACHU2L_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1358727.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1507..>2807)
                     /gene="ndh"
                     /locus_tag="ACHU2L_004127"
     CDS             complement(1507..>2807)
                     /gene="ndh"
                     /locus_tag="ACHU2L_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR1358728.1"
                     /translation="TPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHL
                     WKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKG
                     ELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYS
                     ANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGERI
                     LPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKA
                     PDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQ
                     MATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIAR
                     FVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          677 a          769 c          685 g          676 t
ORIGIN      
        1 tttttataat aacctttcat tcacagtcac caggtacaac ggcatcatgg caactgactc
       61 aacacaatgt gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct
      121 tgataaggcc atgaaattgt tctggcaaca cggttatgaa gcgacttctc ttgcggacct
      181 cgtcgaagca accggagcca aagcgcccac gctatacgcg gaatttacca acaaagaggg
      241 gttatttcgt gccgttctcg accgctatat cgatcgtttt gccgctaagc atgaagcaca
      301 gctgttttgt gaagagaaaa gcgtggagtc tgcgctggct gactattttg ctgccatcgc
      361 caactgcttt accagcaaag acaccccggc aggctgcttc atgatcaaca actgcaccac
      421 cctctcccca gattcaggag atatagccaa tacgttgaaa tcacgccatg cgatgcaaga
      481 gcgcactttg cagcagtttt tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg
      541 tgacgtgaca catctggcag aattccttaa ttgtattatt caggggatgt cgatcagcgc
      601 acgcgaaggt gcatcgctgg aaaaactgat gcagattgcc ggaacgactt tgcgtttatg
      661 gcccgaactg gtgaaataag tcgcaaaaaa ggcccctcag cctgagcgaa tgaggggcca
      721 aattgcagag tacagccttg ttatactttg ttattcaaaa tcagttgccc gttactatct
      781 agcgggatct gcgtacccgg atcgcggtcc atgcggattt tgccctgctg atcgccaatc
      841 ttataggtca catcataacc gagcattttt tctgacttgt catacaccgt tttacaacgc
      901 tgttgcgtag tcgtgtaagt atcgctttcc tggagagagc cctggatctg gttaccggca
      961 tatccaccac ccagcgcccc cacaacagtg gcgacatctt taccgcgacc accaccaaac
     1021 tgatgcccta tcacgccacc agcaacagcg ccgagcaccg acccggtaat gcgattttca
     1081 tcctgcaccg gtcgacgatg ggtcactgtg acgttgcgac actcctgacg cggtgtttta
     1141 accgtttcct tgattggggt tgcagaaaca acctgagcgt attgcgggcc ccgttcaaac
     1201 acgttcagac tggccactgc cgctacgccc aacgcagctg cgacaccaat cccgataccc
     1261 gccaacattg atttattcac gggaacacct ccttctttgc agttatgcca atcatgcaag
     1321 cgaaggagaa aagcagctat cagacagaag atgaaaagcg cgggaatgac tggaaatact
     1381 taaggattcg gaggagtctg aaggaatgcc tgatgcgacg cttaacgcgt cttatcaggc
     1441 ctaccgctcg taccatttgt ccagggcgga taaggcgttt ataccacatc cgccagtgta
     1501 cgtcgattaa tgcaacttca aacgcggacg gataacgcgg ttaatactcc ccaccagcat
     1561 cattaatccg gttttaaagt aaccatgcag cgcaatctga tgcattcggt atagcgagat
     1621 atatacaaag cgcgcaattc gtccttcaat catcattgag ccgcgcgtca ggttacccat
     1681 caggctacca acggtggaga agttcgacag cgataccagc gaaccatgat ctttatactg
     1741 ataatttttc agcggcttac cgttcatctg cgccagaatg ttgttcattg cgcaagtcgc
     1801 catctggtgt gcagcctgag cacgcggcgg aacaaagccc ccttccggac gcgggcatga
     1861 cgcgcagtcg ccaatagcgt aaatgtctgg atcgcgagtg gtttgcagcg tcggttccac
     1921 caccagctgg ttgatacggt tagtttcaag accaccgata tctttcagga agtctggcgc
     1981 tttgatcccg gctgcccata ccatcagatc agcctcaata tattcgccat ctttagtgtg
     2041 caggccgcct tcatcagcac tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt
     2101 cgttagctcg ttgtgggccg cagcagagat acgtggcggt aatgcaggca aaatacgttc
     2161 tcccgcttct accagcgtta cgttcagggc ttcgttggtc aggcctttgt aaccgtagct
     2221 gtgcagttgc ttgatcgcgt tgtgcaattc agcggagagt tctacacccg tcgcgccgcc
     2281 gccgacaatc gcaatgttca ctttgccatt tgcgcccagg ttggcagagt atttcaggaa
     2341 cagattcagc atctcctggt ggaagcgacg cgcctggtgc gggttatcga ggaaaatgca
     2401 gttctctttg acgcctggcg tattgaaatc gttagaggtg ctacccagcg ccatgaccag
     2461 ggtgtcatag gcgattttac gttccggaac caacagttca cctttctcgt cgcgtagttc
     2521 tgcaatagtg attgttttcg cttcacgatc aatatcaatg acggaaccca actggaactg
     2581 gaaaccatga ttgcgcgcat gggccagata gctcaacgca tcgacgcctt catcaagcga
     2641 gccagtcgcc acttcgtgca gcagtggttt ccacaggtgg ctgtggttac gatcgaccag
     2701 cgtaattttg gcttttttct tacgtcccag cttatgcccc agctgtgttg ccatttccag
     2761 cccaccagca ccgccgccga caatcacaat ctttttcaat ggcgtag
//
LOCUS       DAZPEU010000318         2798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPEU010000318 DAZPEU010000000
VERSION     DAZPEU010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.322"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            274..1440
                     /gene="nhaA"
                     /locus_tag="ACHU2L_004128"
     CDS             274..1440
                     /gene="nhaA"
                     /locus_tag="ACHU2L_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR1358729.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1506..2405
                     /gene="nhaR"
                     /locus_tag="ACHU2L_004129"
     CDS             1506..2405
                     /gene="nhaR"
                     /locus_tag="ACHU2L_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR1358730.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2443..>2798)
                     /locus_tag="ACHU2L_004130"
     CDS             complement(2443..>2798)
                     /locus_tag="ACHU2L_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1358731.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          655 a          622 c          706 g          815 t
ORIGIN      
        1 ttggcctaac aagcatcgcc gactgacaac aaattaatta ttacttttcc taattaatcc
       61 ctcaggaatc ctcaccttaa gctatgatta tctgggctta gggtcactcg tgagcgctta
      121 cagccgtcaa aaacgcatct caccgctgat ggcgcaaatt cttcaatggc tcgtaaaaaa
      181 cgaattattc ctacactata atctgatttt aacgatgatt cgtgcggggt aaaatagtaa
      241 aaacgatcta ttcacctgaa agagaaataa aaagtgaaac atctgcatcg attctttagc
      301 agtgatgcct cgggaggcat tattcttatc attgccgcta tcctggcgat gattatggcc
      361 aacagcggcg caaccagtgg atggtatcac gactttctgg agacgccggt tcagctccgg
      421 gttggttcac tcgaaatcaa caaaaacatg ctgttatgga taaatgatgc gctgatggcg
      481 gtatttttcc tgttagtcgg tctggaagtt aaacgtgaac tgatgcaagg atcgctagcc
      541 agcttacgcc aggccgcatt tccagttatc gccgctattg gtgggatgat tgtgccggca
      601 ttactctatc tggcttttaa ctatgccgat ccgattaccc gcgaagggtg ggcgatcccg
      661 gcggctactg acattgcttt tgcacttggt gtactggcgc tgttgggaag tcgtgttccg
      721 ttagtgctga agatcttttt gatggctctg gctattatcg acgatcttgg ggccatcatt
      781 atcatcgcat tgttctacac taatgactta tcgatggcct cccttggcgt cgcggctgta
      841 gcaattgcgg tactcgcggt attgaatctg tgtggtgtac gccgcacggg cgtctatatt
      901 cttgttggcg tggtgttgtg gactgcggtg ttgaaatcgg gggttcacgc aactctggcg
      961 ggggtaattg tcggcttctt tattcctttg aaagagaagc atgggcgttc tccagcgaag
     1021 cgactggagc atgtgttgca cccgtgggtg gcgtatctga ttttgccgct gtttgcattt
     1081 gctaatgctg gcgtttcact gcaaggcgtc acgctggatg gcttgacctc cattctgcca
     1141 ttggggatca tcgctggctt gctgattggc aaaccgctgg ggattagtct gttctgctgg
     1201 ttggcgctgc gtttgaaact ggcgcatctg cctgagggaa cgacttatca gcaaattatg
     1261 gtggtgggga tcctgtgcgg tatcggtttt actatgtcta tctttattgc cagcctggcc
     1321 tttggtagcg tagatccaga actgattaac tgggcgaaac tcggtatcct ggtcggttct
     1381 atctcttcgg cggtaattgg atacagctgg ttacgcgttc gtttgcgtcc atcagtttga
     1441 caggacggtt taccggggag ccataaacgg ctcccttttc attgttatca gggagagaaa
     1501 tgagcatgtc tcatatcaat tacaaccact tgtattactt ctggcatgtc tataaagaag
     1561 gttccgtggt tggcgcagcg gaggcgcttt atttaactcc acaaaccatt accggacaga
     1621 ttcgagcgct ggaagagcgc ctgcaaggca aattatttaa acgcaaggga cgtggtctcg
     1681 aacccagcga gctgggagaa ctggtctatc gctatgccga taaaatgttc accttaagcc
     1741 aggaaatgct ggatattgtg aactatcgca aagaatccaa tttattgttt gacgttggcg
     1801 tggctgatgc actttccaaa cgcctggtca gtagcgtact taacgccgca gtggtagaag
     1861 gcgagcccat tcatcttcgc tgcttcgaat ccacccacga aatgctgctg gagcaattaa
     1921 gtcagcataa actggatatg atcatttctg actgtccgat agactctacg cagcaggaag
     1981 gcctgttctc cgtgagaatt ggcgaatgtg gcgtgagttt ctggtgtaca aatccaccac
     2041 cagaaaaacc gttcccggct tgtctggaag aacggcgact tttgattcct gggcgacgtt
     2101 caatgttagg gcgcaaattg cttaactggt ttaactccca gggattaaac gtagaaatcc
     2161 tcggcgagtt tgatgatgcc gctttgatga aagcttttgg tgcgatgcac aatgcaatct
     2221 tcgttgcccc aacgctttat gcatatgact tttatgccga taaaactgtc gtagaaattg
     2281 gtcgcgtcga gaatgtgatg gaagagtacc atgctatttt tgctgagcgg atgattcagc
     2341 acccggcggt acagcgaatc tgcaatacgg attattctgc gctttttagt ccagcggtgc
     2401 gttaatcggc agctccccca aagttaaggt gggggagata gattagttgt acattaccac
     2461 gattttgact cggctcatta tttgcccgct tgagacattg tttccatatg tacgcgggcg
     2521 aataaataga ggaatctgat tacttccttc atggggatgc tgaaaagagt agtaattgct
     2581 tttaccatac tggatttcat tcgtaccatc attggagagt tgaaagccta tttgacccgc
     2641 accgtcctca ctgttttcta actcattttt taatatgctg gttcctgact cctgtatcgt
     2701 cactggttca aagtaatatt caacgtcact gggttcaaaa actaattcac cttgctcgcc
     2761 ctggatgcag tcaaatctta tatgatgaaa ctgataat
//
LOCUS       DAZPEU010000319         2781 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPEU010000319 DAZPEU010000000
VERSION     DAZPEU010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.323"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..240)
                     /gene="ospB"
                     /locus_tag="ACHU2L_004131"
     CDS             complement(<1..240)
                     /gene="ospB"
                     /locus_tag="ACHU2L_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR1358732.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVF"
     gene            complement(490..609)
                     /locus_tag="ACHU2L_004132"
                     /pseudo
     CDS             complement(490..609)
                     /locus_tag="ACHU2L_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            853..1225
                     /locus_tag="ACHU2L_004133"
                     /pseudo
     CDS             853..1225
                     /locus_tag="ACHU2L_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1787..2734)
                     /gene="icsP"
                     /locus_tag="ACHU2L_004134"
     CDS             complement(1787..2734)
                     /gene="icsP"
                     /locus_tag="ACHU2L_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR1358733.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          808 a          592 c          542 g          839 t
ORIGIN      
        1 aaaaacataa tcatataaca tcttgttctt aaatactctt ggtatttgtt gtcccataat
       61 agatagaaca tcattgctat cacaaaaacc tttctctcct aaaaaaacca atgctgcttt
      121 tgggtgagta catgatgaat tttttaccct ttttattata tgtgcaaatg caatatctga
      181 tatgctttca ttctttttat tgactattct acagtatggt ctaacaccat ctaaattcat
      241 acctacatga ctccatatat tgttttataa acatcatagt aaagggtgtt atattttcat
      301 atagtatgca agcttctttg tatactatga tttaaaaaaa cggaacaaaa atgcaattat
      361 attctggcat tgtttaatgc ggtttgtctt ttttattcat tatttcaatg aaagaatttc
      421 aactacaaag ttaattgatc ctacctacat aatatagaca cagtacctaa agtaccgtgg
      481 agatcatccg gggaagcccc gggtctgata ccagtgcagg tagaccgcta tcttgttccg
      541 gtaatgttct gccgttcttt acggcatcac ctctagcaga aacatcgcgt aactgcgcca
      601 agtatgatga acttctctta ccgctgcgat ctggttcaac aatatgcaga tggtacacaa
      661 cctctttaac cagcccatcc ggattattca gagccgcttc cgcaatccgg aataaaagtg
      721 ctgttttacc gtgaaccctc tgaaaatcag cgagcagtgt tttaaccagc cgacgttcag
      781 agcgtactga cagacggtgg acaatttgaa taaacagttc aatcagaccg tagatgctct
      841 cccgttggcg acatgctggg agcggtggaa caccgcttcg gcaacgagct tccggcgtct
      901 ccagtgaagt gactgacgga taatggttca tgctaccgag ctaatgaaac acgccagttc
      961 acccggatgt tgggactttg ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
     1021 cagaatagca aagagcttcg tgaaaacaat aaagtgtgac tacatcagta tcatgcccaa
     1081 acctgacggg ttaacggcag caaagaacct tgcagaggca ttcgagcatt ataacgaatg
     1141 gcatccgcat agtgcgctgg attatcgctc gccacgggaa tatctgcggc agcgggccaa
     1201 tgataacagg tgtctggaaa tataggggca aagccaccca cgaacgatcc agcatataat
     1261 caagaaatta gatttcattt aacataagat agattatgta caccaagagg cgtttcctta
     1321 atttctgaat ttacaggctc ttttgctctc tttgctatgc tgtggatttg gaaagataga
     1381 gtgctggctg attatttagg tattttcatc agcatgtccc tgatagcact gttccatcag
     1441 cgctttcagg cgataatagt agaccttggg tagcctctgc ccatgtgtaa cttgataccc
     1501 cgagaacagc cacgatgatc taagcggttc ttatttctcc ggtctcaggg tcggtaatgc
     1561 caacagtaag tccacagtaa tcaacgaaga gttttttgaa ccgccccgga aatcctggag
     1621 actaaacgcc ctgagaaaga ggtaaacaga atgactaaaa atacacgttt ttcccccgaa
     1681 gtccgtcaac gggcagttcg tatggttctg gaaagtcagg gcgaatatga ctcacaatgg
     1741 gcggcaattt ttagccatga aaacgtaatc aactcggtga accaattcaa aaaatatact
     1801 ttatacctgc ggaagtgaga aaaccaatat attcaatact tgcactattt ttaatggtgc
     1861 cagatatatt ggtactttgc tcataaagag atgtatcacc ttttttatta gaaatgtaat
     1921 ttctggagcc ttctatataa aattttgcac tgggggtgac atagtatcca atattcgcag
     1981 caacagagca gtaattttgg ttttttattt catctttaaa aattgtctca gtctgatagt
     2041 gtttatctgt atcagatgaa agaacccaac cactatattt cagttctgct ccaaactcaa
     2101 aattctcatg gcggtaatta gcagttaatc caatataagg tattttgaag agctgtttgt
     2161 aacctattgt tctttcacca ctaggatgtg cccctttttt attcctgctc cccccgtttt
     2221 cactataaat ataactccct cccattgcat taaaactgta acgactctct tggtaacctg
     2281 ctattagtcc aagtcgataa tccaaattat ttaataacca acctttaaga ttcagctcga
     2341 attcgttagc atcacggaga tgcgtattgg gatgccagct ctggtcggtc catacctgag
     2401 gggtattgga attgttccag tctttatcaa ccatgcttgc tttctggtta cctaaagtag
     2461 tccaaccgga aactccgaaa gacacttttg gaattagctt ccaatcgatg ccacctctaa
     2521 caatagtcgc attactgtat ttccagtcca gttgactaat ttttctcccc ccttccttgg
     2581 gatgataaac gcgttctttt gttttgccac tcagagatcc tagactaata tccgtactga
     2641 tgttgtccgg tatgaaaagt ggatagttag tggtagcatg tgtagtaaag atcgcaggta
     2701 cacaaagtgc aagtacaaag aattttaatt tcatcgagaa ctctactttt ttggttgaaa
     2761 tgtccatgat cttcttactt t
//
LOCUS       DAZPEU010000320         2755 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPEU010000320 DAZPEU010000000
VERSION     DAZPEU010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.324"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..98
                     /locus_tag="ACHU2L_004135"
     CDS             <1..98
                     /locus_tag="ACHU2L_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1358734.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="ACHU2L_004136"
     CDS             156..512
                     /locus_tag="ACHU2L_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358735.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="ACHU2L_004137"
     CDS             605..823
                     /locus_tag="ACHU2L_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1358736.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="ACHU2L_004138"
     CDS             825..1190
                     /locus_tag="ACHU2L_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1358737.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="ACHU2L_004139"
     CDS             1187..1852
                     /locus_tag="ACHU2L_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358738.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="ACHU2L_004140"
     CDS             1852..2217
                     /locus_tag="ACHU2L_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1358739.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2672..>2755
                     /gene="hokD"
                     /locus_tag="ACHU2L_004141"
     CDS             2672..>2755
                     /gene="hokD"
                     /locus_tag="ACHU2L_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1358740.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKD"
BASE COUNT          781 a          562 c          734 g          678 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctgacgcaga taaattagcc tggatagaag gtgctggcaa
     2461 taaaaaatag cgttttctta tcggtatcgg taagattgct gcgggtgctt gaggctgtct
     2521 gcctcgggca tgccactgta aggcagacag agaaaagccc cagttaacat tacgcgtcct
     2581 gcaagacgct taacattaat ctgaggccca atctatgtct cacaaatgta ggttagcctc
     2641 ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc
     2701 cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagac
//
LOCUS       DAZPEU010000321         2751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPEU010000321 DAZPEU010000000
VERSION     DAZPEU010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.325"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="ACHU2L_004142"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="ACHU2L_004142"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR1358741.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1649..>2751
                     /locus_tag="ACHU2L_004143"
     CDS             1649..>2751
                     /locus_tag="ACHU2L_004143"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1358742.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          708 a          623 c          722 g          698 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt
     1501 ttaatattat catgttaaat gtttatatta taaaaagtcg tttttctgct taggattttg
     1561 ttatttaaat taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt
     1621 gccttcaaat aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc
     1681 agaactactc gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg
     1741 atttccgctg cacgaaatgc gcgatgatgt cgcattccag attatcaatg atgaattata
     1801 tcttgatggc aacgctcgtc agaacctagc cactttctgc cagacctggg acgacgaaaa
     1861 cgtccataaa ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc
     1921 gcaatccgca gccatcgacc tgcgttgcgt aaatatggtt gccgatctgt ggcatgcgcc
     1981 tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcctgtat
     2041 gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggcaaacc
     2101 aacggataaa ccaaacctag tgtgcggtcc ggtacaaatc tgctggcata aattcgcccg
     2161 ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt ttatggaccc
     2221 gaaacgcatg attgaagcct gtgacgaaaa caccatcggc gtggtgccga ctttcggcgt
     2281 gacctacacc ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca
     2341 ggccgacacc ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc
     2401 accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag
     2461 tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga
     2521 cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg
     2581 tacttttgcc atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt
     2641 cctgcgcctc ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc
     2701 ttatctggcg gatgaaatcg ccaaacaggg gccgtatgag ttcatctgta c
//
LOCUS       DAZPEU010000322         2736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPEU010000322 DAZPEU010000000
VERSION     DAZPEU010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.326"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            105..1079
                     /gene="cysB"
                     /locus_tag="ACHU2L_004144"
     CDS             105..1079
                     /gene="cysB"
                     /locus_tag="ACHU2L_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR1358743.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1398..1538
                     /locus_tag="ACHU2L_004145"
     CDS             1398..1538
                     /locus_tag="ACHU2L_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR1358744.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1541..1708
                     /gene="yciX"
                     /locus_tag="ACHU2L_004146"
     CDS             1541..1708
                     /gene="yciX"
                     /locus_tag="ACHU2L_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR1358745.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1822..1917
                     /gene="ymiC"
                     /locus_tag="ACHU2L_004147"
     CDS             1822..1917
                     /gene="ymiC"
                     /locus_tag="ACHU2L_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR1358746.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2081..>2736
                     /locus_tag="ACHU2L_004148"
     CDS             2081..>2736
                     /locus_tag="ACHU2L_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFR1358747.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
BASE COUNT          710 a          633 c          642 g          751 t
ORIGIN      
        1 tagtggttat agttagcacc ttttttatta ttaaatcgta ttagtcaccc gccaggtgtg
       61 acgaaaaaac gatgttctga tggcgtctaa gtggatggtt taacatgaaa ttacaacaac
      121 ttcgctatat tgttgaggtg gtcaatcata acctgaatgt ctcatcaaca gcggaaggac
      181 tttacacatc acaacccggg atcagtaaac aagtcagaat gctggaagac gagctaggca
      241 ttcaaatttt ttcccgaagc ggcaagcacc tgacgcaggt aacgccagca ggacaagaaa
      301 taattcgtat cgctcgcgaa gtcctgtcga aagtcgatgc cataaaatcg gttgccggtg
      361 agcacacctg gccggataaa ggctcgctgt atatcgccac cacgcatacc caggcacgct
      421 acgcgttacc aaacgttatc aaaggcttta ttgagcgtta tccccgcgtt tctttgcata
      481 tgcaccaggg cccgccgaca caaattgctg atgccgtctc taaaggcaat gccgatttcg
      541 ctatcgccac agaagcgctg catctgtatg aagatttagt gatgttaccg tgctaccact
      601 ggaatcgggc tattgtagtc actccggatc acccgctggc aggcaaaaaa gccattacca
      661 ttgaagaact ggcgcaatat ccgttggtga catatacctt cggctttacc ggacgttcag
      721 aactggatac tgcctttaac cgcgcagggt taacgccgcg tatcgttttc acggcaacgg
      781 atgctgacgt cattaaaact tacgtccggt tagggctggg ggtaggggtc attgccagca
      841 tggcggtgga tccggtcgcc gatcccgacc ttgtgcgcgt tgatgctcac gatatcttca
      901 gccacagtac aaccaaaatt ggttttcgcc gtagtacttt tttgcgcagt tatatgtatg
      961 atttcattca gcgttttgca ccgcatttaa cgcgtgatgt cgttgatgcg gctgtcgcat
     1021 tgcgctccaa tgaagaaatt gaggtcatgt ttaaagatat aaaactgccg gaaaaataat
     1081 ttcttgcgtt attttcggca ccttttatgt agcgaaggtg ccggaatata ttctcttttg
     1141 ttacttattt ctataccgtg cacaatattt ttatccctca tatttatagg gtaaattacc
     1201 tgtaaagtag tgtctaatta cccgcctcgc aaattccagt tttcaaatat ctatttcagg
     1261 tcaaaagatt gaattattca ctggagacga ttcgtaaatt cgctggattt ttgactaaag
     1321 tttctttagg atttgtctca tcgtatgatt aattacacca atcattcaac gtcttgctga
     1381 taagtgatgg cgattatatg aggttagcaa tgccttctgg aaatcaggaa ccccgccgtg
     1441 atcctgaatt aaaacgtaaa gcctggctgg cggtttttct tggttctgca cttttctggg
     1501 tggttgtcgc actgctgatt tggaaagtgt ggggataact atggtcggtc aggagcaact
     1561 ggagtcgtca ccattatgcc agcatagtga caatgaacca gaaacgaaac gggaatgttc
     1621 cgtcgttatt ccagacgact ggcaactaac atcgcagcag caagccttta tagaactgtt
     1681 tgctgaagat gatcagccga aacaataatt atcatcattc ttattaccca tttttaatga
     1741 attaaagggc ttttaataca ccgcagcaat aacagcttga gttatctcaa cacaaaataa
     1801 taaccgttaa gggtgtagcc tatgatcaac acaaatatga aatattggtc ctggatgggc
     1861 gcgttttctc tgtcgatgct cttctgggcc gaactcctct ggatcattac tcactgatcc
     1921 ttgaccccgc tgcggcgggg ttgtcatttg ctttgccaca aggtttctcc tcttttatca
     1981 atttgggttg ttatcaaatc gttacgcgat gtttgtgtta tctttaatat tcaccctgaa
     2041 gagaatcagg gcttcgcaac cctgtcatta aggaggagct atgtcgtcaa ccctacgaga
     2101 agccagtaag gacacgttgc aggccaaaga taaaacttac cactactaca gcctgccgct
     2161 tgctgctaaa tcactgggcg atatcacccg tctacccaag tcactcaaag ttttgctcga
     2221 aaacctgctg cgctggcagg atggtaactc agttaccgaa gaggatatcc acgcactggc
     2281 gggatggctg aaaaatgccc atgctgaccg tgaaattgcc taccgcccgg caagggtgct
     2341 gatgcaggac tttaccggcg tacctgccgt tgttgatctg gcggcaatgc gcgaagcggt
     2401 taaacgcctc ggtggcgata ctgcaaaggt taacccgctc tcaccggtcg acctggtcat
     2461 tgaccactcg gtgaccgtcg atcgttttgg tgatgatgag gcatttgaag aaaacgtacg
     2521 cctggaaatg gagcgcaacc acgaacgtta tgtgttcctg aaatggggaa agcaagcgtt
     2581 cagtcggttt agcgtcgtgc cgccaggcac aggcatttgc catcaggtta acctcgaata
     2641 tctcggcaaa gcagtgtgga gcgaattgca ggacggtgaa tggattgctt atccggatac
     2701 actcgttggt actgactcgc acaccactat gatcaa
//
LOCUS       DAZPEU010000323         2734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPEU010000323 DAZPEU010000000
VERSION     DAZPEU010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.327"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            88..153
                     /locus_tag="ACHU2L_004149"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACHU2L_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACHU2L_004150"
     CDS             381..629
                     /locus_tag="ACHU2L_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1358748.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="ACHU2L_004151"
     CDS             complement(745..1014)
                     /locus_tag="ACHU2L_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1358749.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..1739
                     /locus_tag="ACHU2L_004152"
     CDS             1071..1739
                     /locus_tag="ACHU2L_004152"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1358750.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2055..>2734
                     /locus_tag="ACHU2L_004153"
     CDS             2055..>2734
                     /locus_tag="ACHU2L_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1358751.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          835 a          589 c          538 g          772 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
     1381 gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca
     1441 tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca
     1501 atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg
     1561 gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct
     1621 tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga
     1681 atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc
     1741 atccaaccgc agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt
     1801 gcgactttac ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata
     1861 tttaactaac cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag
     1921 tatcgttttt ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa
     1981 tcctgaaaca ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct
     2041 tctgcgaggt tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt
     2101 tttacagaac tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat
     2161 ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat
     2221 gtctggagaa cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac
     2281 gattgcctga caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg
     2341 agctaccaag ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg
     2401 aattaccagt gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg
     2461 aattacctgc gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga
     2521 ggttaccaat gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag
     2581 attttccaga aagagatgat catattataa gaatattctg gcttaatcag aaccggatca
     2641 cggcaattcc ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa
     2701 atcaactatc tcccagaata atgcaaactt tgtt
//
LOCUS       DAZPEU010000324         2729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPEU010000324 DAZPEU010000000
VERSION     DAZPEU010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.328"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..767)
                     /gene="mxiG"
                     /locus_tag="ACHU2L_004154"
     CDS             complement(<1..767)
                     /gene="mxiG"
                     /locus_tag="ACHU2L_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR1358752.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNA"
     gene            complement(776..1234)
                     /gene="ipgF"
                     /locus_tag="ACHU2L_004155"
     CDS             complement(776..1234)
                     /gene="ipgF"
                     /locus_tag="ACHU2L_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR1358753.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(1234..1596)
                     /gene="ipgE"
                     /locus_tag="ACHU2L_004156"
     CDS             complement(1234..1596)
                     /gene="ipgE"
                     /locus_tag="ACHU2L_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR1358754.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(1606..>2729)
                     /locus_tag="ACHU2L_004157"
     CDS             complement(1606..>2729)
                     /locus_tag="ACHU2L_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFR1358755.1"
                     /translation="GFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLS
                     KVVDDEGKEIFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQAL
                     SGKTVDLKIVSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLK
                     EVSVNLKVVTFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEA
                     IEKNPPCKNDVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKS
                     GKDRTGMQDAEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTG
                     VPGNKVMKKLPLSSLELSYSERIGDPKIWNMVKGYSSFV"
BASE COUNT          809 a          607 c          345 g          968 t
ORIGIN      
        1 gcatttttca atttatcaag ttcggtatct tttgaactat ttctttcttt acttaaaaac
       61 accagtaatt ccgctttatc actcactaaa ttcaacacat acaaattgat aaatgggaat
      121 cgttgattga tatattgttg aattctattt atttctttgt ttgacaccag aatatatctg
      181 catttggtaa atccggtttt atttaaggct acagacgccc ataccgcatc attttgtgtt
      241 tcggcaagaa tataattgca ctgtccagtg ttgactattc catatctctt atcatctata
      301 atttcagcaa tttcttttac ttcatctttc tttaaagaga gggaaaaaat tataattaac
      361 acaacaataa cagcaaagaa aaaaaacacc gagtggttct tatacattcc gtttagtata
      421 tggcctctcg atttatcctc cctcatattt ttaaggtgaa aagaaatccc gtcatattca
      481 aatgaattaa attgtgaatt gatatcaatt tgctcacctt tttcatcact aaattgacag
      541 atattatctt tgcttatggt caaataaatt ataccatccg atttactatc tgttacagga
      601 ataatatttt ctgggaaatt gtcgtttcca aactcaagtg tttctatcgt ccgtccgaga
      661 acaattaagt tattcccata atataacgga aactcatccc cgacaccgtt tgtgagtttc
      721 actaataacc tgaatggtgc aagatttgag ttctttgcct cagacataaa tcctcttata
      781 tccttcgatt attctgcttg ctctctgctg ctatccgcaa atagcgtcta tagatatcct
      841 ctgcgtactt taatctttct tttttctttt tcggagacgt tcctgcatta taggctccaa
      901 cagcctccca ccctcttcca tacatcatca taaactcatt taataacttt gctgcataat
      961 gtacagaaag acatggatga ctaattagca tttcctcaga atatcccatt tctctaagtc
     1021 ttttagaatg aaagtcattt atttgcatta taccataatc tttacttcca ttgttattaa
     1081 cattgactgc agatttatta aatccggatt ctttttccgc aatcgctttt aacaaacttg
     1141 atggaatatt gtacctttca ccagccttat cccaacaatc agctctccct aaatatggga
     1201 taaaacacag caaaataaat acgaaacggg acattaatac cccttcattc ttcgcgcaaa
     1261 ttcatccttc agccaacgca cacgcgaaat ataatattct gtattaacat atacatcctc
     1321 gaattcattt atcccggtaa gatctaatcg cgcaattaga tttccgcctt catcatcagt
     1381 agctaagcat atcttttctg agtaatttag gctcaacgca tatataagat catttatatt
     1441 ttcaggcaat gcacaaaaag ggcataataa atttattgaa tctccttctt tttcaatata
     1501 tattagcaca tcatcatcaa gcatgattgc ttgatcatcg atatcaatta aagggatacc
     1561 caaggctcgg caaataacat ctgctaaatc ttccatatat tcctcttata caaatgacga
     1621 ataccctttc accatattcc atatttttgg gtcccctatt ctttcagaat aagatagctc
     1681 taacgaagat agcggcaatt ttttcatgac tttatttccg ggcacaccag tattcatctc
     1741 ttggatttcc atattaccac tattcattag aatagtagaa aataatcttt tctcttctga
     1801 ggataattta ctatttaatt gagaaaactg acctgtttca tgttttctta ttatttctct
     1861 tttaatttca gcatcctgca tacctgttct gtccttccca ctcttacaat tccagcaagg
     1921 tacagcacct atagtataag ccaatagagt cattctttgt gacaatttat acggctctcc
     1981 attatcattt ttttgtaact ttttggttac aatctctttt atctggttag ccagatatat
     2041 cacatcattc ttacatggtg gatttttttc tattgcttct gcagcccaac ctccaatcac
     2101 accatttttg aggaaattat cccccaacaa agaacatatt gattcatcat taagcttgtc
     2161 aacattcctc cagcctaagc ccatttttag cgctaattca tttacaccaa agttaaatgt
     2221 caccactttt agattaacgc ttacttcttt cagaagacca tcactattcc gaattaataa
     2281 tttcgttggc ttcccccttt tagagtttaa cccttttaat gcgttcacct gatcttttaa
     2341 catactctcc tctcccccgg ttaaactggt cggcgtcaga agagaagtcg aaacaatctt
     2401 taaatctact gttttaccag acaaagcctg tgataataac tctggtctgc tatataatgc
     2461 tgcgcttact aactcctcag ctttattacg agcagcaaca gctctttcag atgagttttt
     2521 tttcaatcca taggctgata taactccatg tctgataccc gaaaaaattt ccttcccctc
     2581 atcatccacc acttttgaaa gccacatatt cgcgatatga tcactctctc tcgtagaagc
     2641 acaacaaatt ccttttccat tatactcttt gacaaatata tttttattcc ctatcttcat
     2701 atgagaggca ggagttaatt gaaaaccat
//
LOCUS       DAZPEU010000325         2725 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPEU010000325 DAZPEU010000000
VERSION     DAZPEU010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.329"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(151..1575)
                     /gene="patD"
                     /locus_tag="ACHU2L_004158"
     CDS             complement(151..1575)
                     /gene="patD"
                     /locus_tag="ACHU2L_004158"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR1358756.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1597..2391)
                     /locus_tag="ACHU2L_004159"
     CDS             complement(1597..2391)
                     /locus_tag="ACHU2L_004159"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1358757.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2381..>2725)
                     /locus_tag="ACHU2L_004160"
     CDS             complement(2381..>2725)
                     /locus_tag="ACHU2L_004160"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1358758.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT          698 a          805 c          639 g          583 t
ORIGIN      
        1 aatcaactgt gacaggatga aaaatcgcca tcaaacattt agcctaattt ttacgccagt
       61 tattaatgta atcattctaa tacttaaccg ccattaaact cagaacctaa aacgatagtg
      121 gccgtcctat aacagccact aacatctaac ttaatgttta accatgacgt ggcggacgac
      181 ggtgtaatcc tccagcccat aaagtgacat atccttgccg taaccagaaa gtttctgccc
      241 accgtgcggc atttcactta ccagcatgaa atgggtattg acccaggtac aaccatattg
      301 cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc gtccataccg aagatgcaag
      361 tccgtactgg ctgtcattcg cccagttcac cacctgttct tcgttgtcga agggcgtaac
      421 actcactact ggaccaaata cctctttttg cacgatggca tcgtcctgta atgcgccagc
      481 cagcagcgtc ggcgcatagt aatagccatt acccttgcgc ttttcaccgc cagtgatcac
      541 tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg ctgacgcgtt cgagatgcgc
      601 cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc ggcgcaccag attttaacgt
      661 tgccaccgca gcacccagtt tttccaccag cgtatcgtaa atgccttttt gcgcgtagat
      721 ccgacaagcc gcagtacaat cctgtccagc attgtaatag ccaaatgtac gtacaccttc
      781 gaccactgct tcaatatccg catcatcaaa aacaatcact ggcgctttgc caccaagttc
      841 catatgagta cgcttaatgg acgacgcggt atggctgatg atgtgctcgc cggtggcgat
      901 agagcccgtc agcgacacca tccgcacttt gggatgaccg gtcagcggat cacccaccgt
      961 tttgcctctg ccaaacagta tgttaatcac gcctgccggg aagatatctt tcgccagctc
     1021 tgccaacttc aacgcggtca gcggggtaat ttctgatggt ttaagcacta cgcagttccc
     1081 tgccgccagc gccggagcaa gtttccacgc ggccatcatc agcggataat tccacggtgc
     1141 gatagaagcc acgaccccca acggatcgcg acggatcatc gaagtatgac cttcaagata
     1201 ttcacctgcc gccagaccat tcagacagcg cgccgcaccc gcgaaaaagc gaaaaacatc
     1261 gacaatcgcc gggatttcat cattgaacgc actatgcagc ggtttgccac aattacggga
     1321 ttccagttcg gcaaaaacct gaccattttc ttcgataaca tccgccagtt tcagcaggca
     1381 ttccgcacgc gctttcggtg tggtttgccc ccattcagca aatgctgcat ctgccgcacg
     1441 cacagcagca tcgacctgct ctgcggatac ctcggcaatc tccagtaaaa cgtcccccgt
     1501 tgccggatta tagacaggct gtttttcccc ttcgccgcta acccgttctc cgttaatcag
     1561 taacttatgt tgcatagctt attcctgtat cagtggttat tgaccattgt cgccttcgcg
     1621 ggttagccac caggccccca ggatcggcaa ggttgttacc aacataacca gcagtgccac
     1681 cacgttggtt accggtacat cacgcggtcg cccaagctga ttgagcaacc acaacggtaa
     1741 cgttcgttca tgacctgccg taaaggtcgt aacgatgatt tcatcgaacg acaaggcaaa
     1801 cgccagcatt cctcctgcca gtaacgccga actgagattc ggcaacacta cgtagcggaa
     1861 ggtttgccag ccattggccc caagatccat tgacgcctca accagactcc acgaggtgcg
     1921 gcgaaaacgg gcgatgacat tgttaaacac caccactacg caaaaagtcg catgaccgac
     1981 cacgatggtg aaaaatcccg gctccagatt gatggtttta aaggcggtta atagcgccag
     2041 accagtgaca atgcccggca gcgcaatggg cagcagcaat aacagcgaaa tggcgttttt
     2101 gccgaaaaag tctcgtctcc acagcgcggc agctgccagc gtccctaaca ccagcgcaat
     2161 taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg gcatcaagaa tatcactacg
     2221 ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc ggtggaaaac taaacgccgc
     2281 atcttcagtg ttaaacgcat aggcggcgat tatcaggatg ggaaaatgta ggaaaacaac
     2341 gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt tcagagtgca tcgaacgctc
     2401 ccagacgttt cacgaacgcc aggtacagtg cgatgagaat aatcggcacc agggtgaatg
     2461 ccgccgccat cggcatattg ccaatcgccc cctgctggga ataaaccata ttgccgataa
     2521 aatatcctgg aggaccaacc agctgcggga cgataaaatc gcccagtgtg agtgagaagg
     2581 taaagataga gccagcggca atacccggga ttgccagcgg cagcaccaca tagcgaaagg
     2641 tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa caatgacggc ggcaaacgct
     2701 caagcgccgc ctgaacgggc aggat
//
LOCUS       DAZPEU010000326         2715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPEU010000326 DAZPEU010000000
VERSION     DAZPEU010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.330"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..338)
                     /locus_tag="ACHU2L_004161"
     CDS             complement(<1..338)
                     /locus_tag="ACHU2L_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR1358759.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="ACHU2L_004162"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="ACHU2L_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(992..1338)
                     /locus_tag="ACHU2L_004163"
                     /pseudo
     CDS             complement(992..1338)
                     /locus_tag="ACHU2L_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1445..2008)
                     /locus_tag="ACHU2L_004164"
     CDS             complement(1445..2008)
                     /locus_tag="ACHU2L_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR1358760.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT          835 a          600 c          541 g          739 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acaga
//
LOCUS       DAZPEU010000327         2712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPEU010000327 DAZPEU010000000
VERSION     DAZPEU010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.331"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACHU2L_004165"
     CDS             50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACHU2L_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR1358761.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2712
                     /locus_tag="ACHU2L_004166"
     CDS             2583..>2712
                     /locus_tag="ACHU2L_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1358762.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          726 a          585 c          678 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc gg
//
LOCUS       DAZPEU010000328         2693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPEU010000328 DAZPEU010000000
VERSION     DAZPEU010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.333"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..593)
                     /locus_tag="ACHU2L_004167"
     CDS             complement(<1..593)
                     /locus_tag="ACHU2L_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1358763.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHU2L_004168"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHU2L_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR1358764.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACHU2L_004169"
     CDS             complement(1422..1961)
                     /locus_tag="ACHU2L_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1358765.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHU2L_004170"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHU2L_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR1358766.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          696 a          690 c          591 g          716 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa cat
//
LOCUS       DAZPEU010000329         2692 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPEU010000329 DAZPEU010000000
VERSION     DAZPEU010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.334"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..893
                     /locus_tag="ACHU2L_004171"
     CDS             <1..893
                     /locus_tag="ACHU2L_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR1358767.1"
                     /translation="ELARVGVNPHDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQ
                     DKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRL
                     DRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSS
                     PSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLD
                     QAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQ
                     HPELSIDFINRYGYLPSLGQ"
     gene            894..2624
                     /locus_tag="ACHU2L_004172"
     CDS             894..2624
                     /locus_tag="ACHU2L_004172"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1358768.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT          757 a          661 c          707 g          567 t
ORIGIN      
        1 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
       61 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
      121 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
      181 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
      241 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
      301 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
      361 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
      421 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
      481 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
      541 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
      601 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
      661 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
      721 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
      781 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
      841 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
      901 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
      961 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     1021 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     1081 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     1141 agtttggtaa ctggcgtgac tctcttcagg cttatcacga cggtcccggc aacgttatga
     1201 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     1261 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     1321 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     1381 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     1441 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     1501 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     1561 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     1621 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     1681 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     1741 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     1801 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     1861 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     1921 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     1981 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     2041 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     2101 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     2161 atctcgcttc atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg
     2221 ctgaaaggct taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca
     2281 aggcagttga gcaagcaatc aagtcccgtg cgggttctgc tctacaggtg caggaaaatg
     2341 aagcaatcag gcagctagca cagggcgcgg gtgagataat tgatcgcgtt tccggtgcaa
     2401 aagatgctct tggtatgagc gacaagttta ttgatacggt caatggaaga atgtctgcgc
     2461 tgatgaaacg aagcgaccag ctttatcgca atgttgaaaa ggcgatgcct gcaggtgcaa
     2521 aaattgatgc gccatcaaca aggtcaatgc tcaaacaggt ggcagaagat cttggcggga
     2581 tgaaaaacct tgaccctatt gaaaagagag ttgaggtagc ctgagtttaa cggacactcc
     2641 ttcctgaaat agaatggcat cagaaggagc taataatgag cagaaaaacc ca
//
LOCUS       DAZPEU010000330         2625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPEU010000330 DAZPEU010000000
VERSION     DAZPEU010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.335"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..608
                     /gene="mxiC"
                     /locus_tag="ACHU2L_004173"
     CDS             <1..608
                     /gene="mxiC"
                     /locus_tag="ACHU2L_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR1358769.1"
                     /translation="KSVNAGINSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQ
                     YIEWLGNFGFNHRHTIVNFVEQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDV
                     IFMKKLESSSLLKDGSLSAEQLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQ
                     TYLSSVNESPHDIFKSESEREIAINILRELVTSAYKKELSR"
     gene            621..>2625
                     /gene="mxiA"
                     /locus_tag="ACHU2L_004174"
                     /gene_synonym="virH"
     CDS             621..>2625
                     /gene="mxiA"
                     /locus_tag="ACHU2L_004174"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR1358770.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILV"
BASE COUNT          814 a          355 c          539 g          917 t
ORIGIN      
        1 aaaaaagtgt taatgctgga ataaattcag ctatacaggc taaattattt ggcaacaaaa
       61 tgaaacttga accacagctt ttgcgtgcat gttatcgtgg ttttatcatg gggaacatat
      121 caacaacaga tcagtatata gaatggcttg gtaattttgg ttttaatcac agacatacaa
      181 ttgtgaattt tgtagagcag tcactgattg tagacatgga ttctgagaaa ccgagctgta
      241 atgcttatga gtttggtttt gtgttatcta aattaattgc aattaagatg attagaactt
      301 cagacgtaat ttttatgaag aaactggaat cctcaagctt gctaaaagat ggcagtttaa
      361 gtgcagagca gctattgcta actttattat atatttttca atatccaagt gaaagtgagc
      421 aaattcttac ttctgttata gaagtatcac gagccagtca tgaggattct gtagtgtatc
      481 aaacatatct atcttctgtt aatgaaagtc ctcatgatat atttaaaagt gaaagtgaaa
      541 gagaaattgc gatcaatatt ctacgagagc ttgtcacaag tgcatacaag aaagagcttt
      601 ctagataaca ggagataaaa gtgatccagt cttttcttaa gcaagtaagt actaagcctg
      661 aattaattat attagtgctt atggttatga tcattgctat gttgatcata ccattaccta
      721 cataccttgt tgatttttta atagggctta atattgtact tgctattctc gtttttatgg
      781 gatcatttta tattgaaaga atattgagtt tttctacatt cccttcggtt ttgttgataa
      841 cgacattatt tcgtctcgct ttatcaataa gtactagtcg actaatttta gttgatgcgg
      901 atgcgggtaa aattattact acatttggcc agtttgtcat tggtgatagt ctggctgtag
      961 gttttgttat tttttcaata gtaactgttg tgcagttcat tgttataaca aaaggatctg
     1021 aaagggttgc ggaggtagct gctcgctttt ctcttgacgg tatgccgggg aaacaaatga
     1081 gcattgatgc ggatttaaag gctggaatta ttgatgccgc aggagctaaa gaaagacgca
     1141 gtattctgga acgtgagagt caattgtatg gttcatttga tggagcaatg aagttcatta
     1201 aaggtgatgc aattgctggc atcattatta tttttgtcaa tttaatagga ggtatttctg
     1261 ttggtatgag tcaacatgga atgtccctat ctggtgcatt atctacttat accatactaa
     1321 ctattggtga cggattggtc tctcagattc ctgctctgtt aatttctata agtgcaggat
     1381 ttatcgttac aagagttaat ggtgatagcg ataatatggg acgtaacata atgtcccaga
     1441 tatttgggaa tccttttgtt ctcattgtta catcagctct tgccttggcg atagggatgt
     1501 tgccaggttt tccgtttttt gtttttttcc tgatagcagt tactttgacg gctttattct
     1561 attataaaaa ggtcgtagaa aaagaaaaaa gtctgtctga gtctgattct agtggctata
     1621 ctggtacatt tgatattgat aatagccatg actcatcttt ggcaatgata gaaaatctgg
     1681 atgctattag ttcagaaacc gttcctttaa ttttattatt tgccgaaaat aagataaatg
     1741 caaatgatat ggaaggtctt attgaaagga taagaagtca gttctttatt gattatggtg
     1801 tcaggcttcc aactatttta tatagaacaa gcaatgagct taaggtcgat gatattgttt
     1861 tgttaataaa tgaggtgcgt gccgatagtt ttaatatata ttttgacaaa gtgtgtatta
     1921 cagatgaaaa tggagatata gatgctctag gtattcctgt tgtttcaact tcatataatg
     1981 aacgtgttat ttcttgggta gacgtttcat atacagaaaa tctaactaat attgatgcta
     2041 aaattaaaag tgctcaagat gaattttatc accagttgtc acaagcttta ttaaacaaca
     2101 taaatgagat ttttggtata caagaaacaa aaaatatgtt agatcagttt gaaaatcggt
     2161 atcctgatct attaaaggaa gtcttccgac atgtgactat acagagaatt tctgaggtat
     2221 tacaaagatt gcttggagaa aatatttctg ttcgcaattt aaaacttatt atggagtctt
     2281 tggcgctttg ggctcccaga gaaaaagatg tcataacatt agttgaacat gtccgtgcat
     2341 cactttctag gtatatttgt agtaaaatag ctgtttctgg tgagattaaa gttgtgatgc
     2401 tttccggata tattgaggat gcaataagaa aagggataag gcaaacctct ggtggctctt
     2461 tcttgaatat ggatatagag gtttcggatg aggtaatgga aactttagca catgctttga
     2521 gagaattgag aaatgcaaaa aaaaatttcg ttcttttggt atcagtagat atacgtaggt
     2581 ttgttaaaag acttatagat aacagattta agagtatact cgtta
//
LOCUS       DAZPEU010000331         2601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPEU010000331 DAZPEU010000000
VERSION     DAZPEU010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.336"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..198
                     /locus_tag="ACHU2L_004175"
     CDS             <1..198
                     /locus_tag="ACHU2L_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1358771.1"
                     /translation="KLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIK
                     QRGVNVFIQDVPGDQKEQIPD"
     gene            237..1040
                     /gene="agaC"
                     /locus_tag="ACHU2L_004176"
     CDS             237..1040
                     /gene="agaC"
                     /locus_tag="ACHU2L_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR1358772.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1030..1821
                     /gene="agaD"
                     /locus_tag="ACHU2L_004177"
     CDS             1030..1821
                     /gene="agaD"
                     /locus_tag="ACHU2L_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR1358773.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1822..2577
                     /locus_tag="ACHU2L_004178"
     CDS             1822..2577
                     /locus_tag="ACHU2L_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR1358774.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          629 a          613 c          663 g          696 t
ORIGIN      
        1 aaattggtag aaggtggcat tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg
       61 gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct cgcggactta
      121 cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa
      181 gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc
      241 atgaaataac cctacttcag ggattatccc tagcggcgtt agttttcgtt ctggggattg
      301 atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc ctaactggcg
      361 ctattctcgg tgatattcag actggcttaa ttaccggtgg tctgacagag ttggctttcg
      421 ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc gattatggcg ggtctgatga
      481 ccaccgtcat tgcatggtct acgggcgttg atgccaaaac agcaattggt cttggcctgc
      541 cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta
      601 tgaccaaagc cgataaatgc gcgaaagagg cggatacggc agcgttttcc cggcttaact
      661 ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc
      721 tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg acccacggct
      781 ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt
      841 tcaaagcgca atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccggg
      901 tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca
      961 atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg
     1021 actacagcaa tgggatctga aatcagtaaa aaagatatca cccgtctggg ctttcgttcg
     1081 tcgctgctgc aagcgagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg
     1141 atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcttaag cgcggcgatg
     1201 aaagataacc tcgaatttat taatacccac ccgaatctgg tcggattcct gatggggtta
     1261 ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggca
     1321 ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacttt gttgccgatt
     1381 atggcgggaa tttgctcatc atttgccagc cagggaaacc tgctggggcc gattctattt
     1441 ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca
     1501 gtcggcgtga aggcgatcga taaagtgcga gagaactcgc agatgattgc tcgctcggca
     1561 accatcctcg ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg
     1621 gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcaacagga tttcttcgat
     1681 aaagtcttcc cgaacatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg
     1741 gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt
     1801 tccgcattcg gcattttgta aatggaacga ggcactgctt ctggcggtgc ctctttactt
     1861 aaggaatttc atcctgtgca aacccttcag caagttgaaa actatacggc gttaagtgaa
     1921 cgagccagcg aatatttatt ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg
     1981 gcgaccggag ccacgccatt actgacgtat cattatctgg tagaaaaaat ccaccagcag
     2041 caggttgata tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
     2101 acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagcc gctggggcta
     2161 cgtgaagacc agctcatcag ctttcgctcc gaagagataa atgagacaga gtgcgaacgg
     2221 gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctcgg attggggaaa
     2281 aacggtcatc ttgggctgaa cgaaccggga gaaagcctac aaccggcctg ccatatcagt
     2341 caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact
     2401 cgtgggatca ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt gttattggtg
     2461 actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct
     2521 atcccggctt catttttatg gctgcacagt aactttattt gtttaattaa tacctaacca
     2581 gatgaaaaat ttgctcaaaa t
//
LOCUS       DAZPEU010000332         2565 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPEU010000332 DAZPEU010000000
VERSION     DAZPEU010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.337"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            124..375
                     /locus_tag="ACHU2L_004179"
     CDS             124..375
                     /locus_tag="ACHU2L_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1358775.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            377..2473
                     /locus_tag="ACHU2L_004180"
     CDS             377..2473
                     /locus_tag="ACHU2L_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR1358776.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT          717 a          598 c          707 g          543 t
ORIGIN      
        1 ttaaaaaata aaccataaaa acaccaaaga accctttgca gggaatcttt ggtatcttaa
       61 tctttatcca tacggatatc cgaccggata tccgcgcggg taattttgta ggttaaagga
      121 aagatgagac aaagagaaga tagtgaccgc gattggcctt cgctgaagat tcagctggcg
      181 ctcaaagata acgggtggtc ttccttccgg gagctttcac tccatgctgg ttatgctcag
      241 gattcactga aagcggtagt tcgcacacca aacaaaaaat acgaacgcgt aatttctgac
      301 gctataggtg taccgccaga aatcatctgg cccagccggt atcgtaacga tggatttatc
      361 aggaaggcgt cgtgatatgc agtcgtacgt taccgtaaat gacctgctcg gcgtaccagg
      421 aatgccagcc accacaaaag gcatccgaca ggcgttacag cgtttttctc gtgatcttgg
      481 tgacgttagt cgccgtcggg aaggcacaaa agccattgag taccacattg attgtctgcc
      541 tgaaataaca cgaaaggcac tgcgtgaacg ctatgtggaa cagctggtgg ctacagagaa
      601 caatgtttct gaagtgaaag ctgttaccag aaaaacacgc aatcctgacg ctgtccaggc
      661 aatcgaagca tatcgcggtt ctccacaact gatggaagaa cgcctgaatg cgctgaccga
      721 aaaccagcgc tgggtatctg aagcaagagc tgcgctggtg gtggaagtgc tgaagctgga
      781 aagcgccggt aaccccgggc gactgaaagc cattaacttt cttgttgaaa aagcccgtaa
      841 aggtgagctg ccggagcgcc tgcaacaggc cgcagttaac gccaatgcaa aacgtggcgc
      901 taatcgtact atcagccgcg atccacttta ccagtgggtt ctgaaataca accagtcgca
      961 gaacgccgcc gaacggctgc tgttactggc accgggaaaa cgcgacgaaa taaaaccgga
     1021 agaaatcagc tggctgcctg aatttctggc gcagtatcgc caggtgaacg gcagacccat
     1081 gtcagaagcc tacgaggatt ttgtcgcaga atggcagcgt cgccatgcgg acgaaccgta
     1141 catgctggag gtgatgcctt cctatgatgt tgtccggtat gccatgaaaa aactgccgga
     1201 agtggtgaaa caaaaagggc gcgttaccgg cagtgaatac cgtcagcttg aaggattcac
     1261 ccgccgcgac tggacagcta tgccggtgaa ttatgtctgg attggtgacg gtcacggtat
     1321 gaagctgaaa tgtgcgcatc cgatccacgg gcgtccattc tcaccggaag tcacgtttgt
     1381 gatcgatggt ggaacacggt ttgttgttgg ctggagtctt gaccttgccg aaaacgtgtt
     1441 cgccgttgcc ggtgcgatac agcacggcat tcgcaaccac ggaaaaccgt tcctgtatta
     1501 ctcggataac ggttccggtg aaacggctga catgctggac aaagaggtcg tgggtattct
     1561 gccacgcctg gggattaaac accctaccgg tattgccggt aatccacagg gacgcggcat
     1621 tattgaacgc ctgaaccgga cactgccgat gcgtatagcg aggaggtatc gcacctattt
     1681 tggcaagggt gctgaccggg aatcgttgcg tgtacttaac cgtgatctgc gttcagcgtt
     1741 taacgccctg caacaggaca agcctctgaa cgaccgccag aaagcggcaa tgcgcgagct
     1801 gccatcgtgg gcggagctta ttgaggctat tcgtgaaggc gtggaatggt acaacaaccg
     1861 cccgcattct gagctgccga tgaaaccgaa cggacggcat tacagcccga cagagttcag
     1921 aaaaaaacgc caggcagaag aagacaccga aattgagtgg ctgagtgatc ttgagctgcg
     1981 cgacatgttc cgcccaatgg tggaacgacc ggtaagacgc tgcgaaattc agtggctgaa
     2041 caatatttac tacgcgccgg agttgcgtga cgaacacggt cgcaaagtgc ttatcagtta
     2101 tgacattcat gacgccgagc gaattacggt acgtcgcaag gacggcagct ttatctgtga
     2161 ggcgatatgg aacggcaata aacgcgcagc ctttgctgtc agtgctgaat atcacaaaca
     2221 acagcagcgt attaaaggaa tgcgcaaacg cgctgaggaa aaaattcgtg atgctgaaga
     2281 tgaaggtatt cagattctgg aacacaaaca ggctgaaccc tggctgagta atgtttatcg
     2341 cccggttggt aatgtggtgg cagttcagca accggaatat gaagaagagc atgacgaaga
     2401 atttgaacgc gatttccggc tggggatgca aaaacttttt gccatgcagg aagaagatga
     2461 tccgctggcc tgaataaaaa aacagtccgc gggaacggac tgttaaataa aggaaacaaa
     2521 gctgatttga aataaccgag gaaatactat gactgatatt gatga
//
LOCUS       DAZPEU010000333         2534 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPEU010000333 DAZPEU010000000
VERSION     DAZPEU010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.338"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..565
                     /locus_tag="ACHU2L_004181"
     CDS             <1..565
                     /locus_tag="ACHU2L_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1358777.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="ACHU2L_004182"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="ACHU2L_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR1358778.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACHU2L_004183"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACHU2L_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR1358779.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="ACHU2L_004184"
     CDS             2167..2403
                     /locus_tag="ACHU2L_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR1358780.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2442..2528)
                     /locus_tag="ACHU2L_004185"
     tRNA            complement(2442..2528)
                     /locus_tag="ACHU2L_004185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2492..2494),aa:Leu,seq:cag)
BASE COUNT          650 a          565 c          652 g          667 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagataa ttggtgcgag gggggggact
     2461 tgaaccccca cgtccgtaag gacactaaca cctgaagcta gcgcgtctac caattccgcc
     2521 accttcgcac agtc
//
LOCUS       DAZPEU010000334         2530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPEU010000334 DAZPEU010000000
VERSION     DAZPEU010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.339"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            113..202
                     /locus_tag="ACHU2L_004186"
                     /pseudo
     CDS             113..202
                     /locus_tag="ACHU2L_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            504..1499
                     /gene="pgl"
                     /locus_tag="ACHU2L_004187"
     CDS             504..1499
                     /gene="pgl"
                     /locus_tag="ACHU2L_004187"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1358781.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1540..2493)
                     /locus_tag="ACHU2L_004188"
                     /pseudo
     CDS             complement(1540..2493)
                     /locus_tag="ACHU2L_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          635 a          575 c          627 g          693 t
ORIGIN      
        1 taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag gagatcaccc
       61 aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga ccatgcttat
      121 tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta atgcgtttat
      181 ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt tggccagaag ttgttatggc
      241 atagtgttta cggtcgttga tacaggtaaa caaaagcggg gagagtcata ctccctgaat
      301 agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg tgactaatat tttagtaata
      361 tgccattcga aatggagtgc taacttattg atttaattga tgtgcgatat tgtattttat
      421 cgtgttttgt tgtctgtttt ttatccagta agtgttgtta tatgtatgaa agataaggga
      481 tttatcattt ttgaggtgtg ttcatgaagc aaacagttta tatcgccagc cctgagagcc
      541 agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca caggttgtcg
      601 atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat ctctatgttg
      661 gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc cccggacgat ggcgcactga
      721 cctttgccgc agagtctgtg ctgccgggta gtccgacgca tatttccacc gatcaccagg
      781 ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg cgtctggaag
      841 atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct ggacggttgc cattccgcca
      901 atatctcacc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat cgcatttgcc
      961 tgtttacggt cagcgatgat ggtcatctgg tggcgcagga ccctgcggaa gtgactaccg
     1021 ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat gcgtattgcg
     1081 tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac ggcaatatcg
     1141 aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt tgggcggctg
     1201 atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc gccagtctga
     1261 ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc ttccagccaa
     1321 cggaaaccca gccgcgcggc ttcaatgttg attacagcgg caagtatctg attgccgctg
     1381 ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcagggg ctactgcatg
     1441 aaaaaggccg ctatgcggtc gggcagggac caatgtgggt ggtggttaac gcacactaac
     1501 cgctgattta accggcgcag tctctcctgc gccggtgtat taacttatct cctgtaacgc
     1561 atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag tccacaaatt
     1621 aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc acggcatctt tgcggcgaat
     1681 aatccccaat gtccgacgta taacgggttc caccagtggg ataccaataa tcgaagaata
     1741 gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcctg cctccactag
     1801 tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc agggcttgtc
     1861 ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc caatcatttt
     1921 gtaacccact aactcctgcc attctacaag ctactttttg gccaatggat ggtcacgccg
     1981 acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg aatttgtgac
     2041 gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca tgcaattatt
     2101 cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa atttaccgat
     2161 tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaatgtca ccataccacg
     2221 atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct ttattaattc
     2281 ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc gagttgtacg
     2341 ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg ttaatgcagg
     2401 ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa ctggactctg ccagtatgac
     2461 aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg gaagcgacga
     2521 ttttgggggg
//
LOCUS       DAZPEU010000335         2487 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPEU010000335 DAZPEU010000000
VERSION     DAZPEU010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.341"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>2487
                     /locus_tag="ACHU2L_004189"
     CDS             <1..>2487
                     /locus_tag="ACHU2L_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1358782.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQE"
BASE COUNT          728 a          577 c          602 g          580 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggag
//
LOCUS       DAZPEU010000336         2469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPEU010000336 DAZPEU010000000
VERSION     DAZPEU010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.342"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..392
                     /gene="capU"
                     /locus_tag="ACHU2L_004190"
     CDS             <1..392
                     /gene="capU"
                     /locus_tag="ACHU2L_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR1358783.1"
                     /translation="QYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGMV
                     LAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQMA
                     RLAKQDIEERFDINKTALKILTLAKQK"
     gene            397..1347
                     /gene="virK"
                     /locus_tag="ACHU2L_004191"
     CDS             397..1347
                     /gene="virK"
                     /locus_tag="ACHU2L_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1358784.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSGYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1412..2356
                     /gene="lpxM"
                     /locus_tag="ACHU2L_004192"
                     /gene_synonym="msbB"
     CDS             1412..2356
                     /gene="lpxM"
                     /locus_tag="ACHU2L_004192"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1358785.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          716 a          475 c          558 g          720 t
ORIGIN      
        1 tacagtatca gattgacagt atgggcatgc atgatgatgt ttttattgct gacaatgttt
       61 ttcctgccgc ccccgtatat cgggttgcca gtctggtggt tctgccttca gaaaacgaat
      121 cttttggtat ggtgctggca gaagcatcgg cattttctgt gcctgtagtg gccactcaga
      181 ttggtggaat ccctgaggtt attcagaaca accagaccgg gacattgtta ccagcaagta
      241 ataagcacgc atggatgtgc gccctgaatg atttttttaa tgaccctggg cgtttttatc
      301 agatggctcg cctggcaaaa caggatatag aagagcggtt tgatattaat aaaactgcgt
      361 taaaaatact cacattagcg aagcaaaagt aacaatatgt tttctataag taacttatca
      421 tttatcggtt tccttaaaag gattattttt tcctcagatt cactaccggg gaagtgggaa
      481 cacagaaaat ttcggttcat gtacattttg cgatgttcta taaatccggt tgtcagtatt
      541 cgatattatt atgaactgcg ttccttgccg tgcattgagg atattctggc aatacacccc
      601 acgttgccag caagaattca tcgcccttat ttgcataaag gggggcgcgc ctggacccgt
      661 ggacaatata ttcttgagca ttatcgcttt gtgcaaaatt tgccagaaaa atattctaaa
      721 tttttgtttc cacaaaaatc ggtatcatta gtccaattca tcgggaaaga tggagagaat
      781 tttgatatcc agtgttctcc cagtggtttt gaccgggaag gagagttgat gttgtcattg
      841 ttttacaata aaacagttat tgcccggtta acattttcag tcattctgac tcaaaatgga
      901 cacattgctt ttattggcgg attacaggga gcccctaaaa ataccggacc tgatgttatt
      961 cggtgcgcaa cccgggcctg ttatgggatt ttcccaaaac ggattatttt tgaagcattt
     1021 tgtgcgttga tgaaagcttg caatatctct gagtgtctgg cagtcagtga gcacagtcat
     1081 gttttccggc aattgagata ctggtatcag aaacgcaaaa cctttgttgc ggtctatagt
     1141 gatttctggg agtctgtagc aggaaaaacc tgtggtgact ggtacagatt accaacccag
     1201 gtgattcgaa agccgctaag cgatatagcc agtaaaaaac gctctgggta ccgaaaacga
     1261 tacgctttgc tggattatat ccatgaaact actatccgct ctttggatgc atatcctgta
     1321 cactcagaac atcagaattt aaattaaggt ttgatgtatt ctctgaataa aacagtaatg
     1381 ataattttgg tagaggcatt cgcactaaat aatgaaaaaa tataaatctg agtttattcc
     1441 tgaatttaag aaaaattacc tttcccctgt ttactggtct acatggttcc ttttgggaat
     1501 gattgcaggt atttcaatgt ttcccccttc attcagagat cctgtcttgg caaaaatagg
     1561 gcgttgggcg ggcagattga gcaaaaaagc tcgtcgcagg gcgacgatta atttatcgct
     1621 ttgtttccca gaaaagagtg atacagaacg ggaaataatt gtcgacaaaa tgtttgtcac
     1681 agcattgcaa tccatagtga tgatggcaga actggcaatt cgtggtccgg aaaagttcca
     1741 gaaacgagtg ttctggaaag gcctcgaaat tcttgaggaa attcgacaca ataatagaaa
     1801 tgtgattttt ctggttcccc atggctggag cgtggatatt cctgcaatgt tgctggcagc
     1861 tcagggaaaa aaaatggccg ccatgtttca tcagcaacga aatccagtga ttgattatgt
     1921 ctggaattca gtacggcgta aattcggggg gcgcttacat gcccgggagg atgggataaa
     1981 accatttatt cagtcagtac gccagggata ctggggatat tacctcccag atcaggatca
     2041 tggtcctgaa tacagtgaat ttgctgattt ttttgcgacc tataaagcga cattaccaat
     2101 cattggacgt ctgatgaaca tcagtcaggc tatgattata ccacttttcc cggtttatga
     2161 tgaaaaaaaa catctactga ctattgaaat tcggccacca atggatgctt gcattgccag
     2221 cgcggacaat aaaacgattg cccgacaaat gaacaaaaca gtggaaattt tggtggggcc
     2281 acatccggaa cagtatgtct gggttttaaa attgttaaaa acacgcaaat caaacgaagc
     2341 ggacccgtac ccttgaaatt ataaagtaca agtatttcca ctagttgttt gtaagcgtac
     2401 agcctgaacc gtctggtcag aatctgacga attagacaaa gtggtgtcca ccaaataagt
     2461 agtgggaac
//
LOCUS       DAZPEU010000337         2463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPEU010000337 DAZPEU010000000
VERSION     DAZPEU010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.343"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            112..447
                     /gene="eutS"
                     /locus_tag="ACHU2L_004193"
     CDS             112..447
                     /gene="eutS"
                     /locus_tag="ACHU2L_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR1358786.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            460..939
                     /gene="eutP"
                     /locus_tag="ACHU2L_004194"
     CDS             460..939
                     /gene="eutP"
                     /locus_tag="ACHU2L_004194"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR1358787.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            914..2287
                     /gene="eutA"
                     /locus_tag="ACHU2L_004195"
     CDS             914..2287
                     /gene="eutA"
                     /locus_tag="ACHU2L_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR1358788.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
BASE COUNT          559 a          628 c          704 g          572 t
ORIGIN      
        1 tctccattct tattgatcta cctcaccttt taaacgcgct tgccgaattt tgttatttac
       61 tctgacgaaa aattgtcacg atacacgaaa gtttttcaca ggcggcgact catggataaa
      121 gaacgcatca ttcaggaatt tgtgcccggc aaacaggtca cactggcgca tctcatcgcg
      181 catcccggtg aggaactggc gaagaaaatc ggcgttccgg atgcgggcgc aattggcatt
      241 atgacgctaa ctcccggcga aacggcgatg atcgccggtg atttagcgct gaaagccgct
      301 gatgtgcata tcggttttct cgaccgcttc agcggtgcat tggtgattta tggctcggtg
      361 ggcgcagtag aagaagcgtt atcacaaacc gtcagcggtt taggtcgctt attaaattat
      421 acgctttgtg aaatgaccaa aagttaatac gaggcattca tgaaacgaat tgcgtttgtg
      481 ggtagcgtcg gggcaggaaa aacaacgtta tttaatgcgt tgcaggggaa ttataccctc
      541 gccagaaaaa cactggccgt ggaatttaat gataagggcg atattgatac gccgggtgaa
      601 tattttagcc atccccgctg gtatcacgcc ttaattacca cgctacagga tgtagacatg
      661 ctgatctatg tccatggcgc aaatgacccg gaaagtcggt tgcctgccgg gttgctggat
      721 attggcgtca gcaaacggca aatcgccgtc atcagtaaaa cggacatgcc agatgctgac
      781 gtcgccgcaa cgcgaaagtt attgcttgag acgggtttcg aagagccgat tttcgagcta
      841 aacagccacg acccgcaaag cgtgcagcaa ctggtggatt atctggcatc tctcaccaaa
      901 caggaggaag caggtgaaaa aactcatcac agcgaatgat attcgtgaag cacacgcgcg
      961 cggcgaacag gcaatgtccg tggttctgcg cgccagcatt attaccccgg aagcccgtga
     1021 agtggcggat ctactgggtt ttaccatcac cgaatgtgat gaatcgatcc cggtaacggc
     1081 gtccgtgccc gccagcgtgc cagccgacaa aaccgaaagc cagcgcatcc gcgaaaccat
     1141 cattgcccag ctgccggaag gccagtttac cgaaagcctg gtcgcacagc tgatggaaaa
     1201 agtgatgaag gaaaaacagt cgctgggcga ttttgtcgtc gccagcgccg ggccgcacct
     1261 cgaatccgtg atcgccggtc acggagccgg ggcgcaaacc ctttctgaac aacggctgtg
     1321 tcgggtactg aatatcgata tcggcggtgg caccgcgaac tacgccctgt tcgatgccgg
     1381 aaaaatcagc ggcactgcct gtctcaacgt cggtggtcgc ctgctggaaa ccgacagcca
     1441 ggggcgcgtg gtttacgctc ataaaccggg gcagatgatt gtggatgagt gcttcggtgc
     1501 aggcactgac gcccgttcgc tgaccggcgc gcagctggtg caggttaccc ggcggatggc
     1561 ggcgctgatt gtcgaagtga ttgacggaac gctttcgccg ctcgcgcagg cattgatgca
     1621 aaccggtttg ctgcccgcag gtgttacgcc cgaaatcatt acgctttctg gaggcgtggg
     1681 cgaatgttat cgccaccagc ccgccaaccc gttctgtttt gccgatattg gcccacttct
     1741 ggcaacggcg ctgcatgacc atccgcgcct gcgtgagatg aatgtgcagt ttccggcgca
     1801 aaccgtacgc tccacggtga ttggcgcggg tgcacatacc ctttcgctct ctggcagcac
     1861 aatctggctg gagggcgtac aactgccgct gcgcaatttg ccggtggcga tcccgattga
     1921 tgaaacggat ctggtgagtg cctggcaaca ggcgctgctt cagctggatc ttgatcccaa
     1981 aactgacgtg tacgtgctgg cgcttcccgc ctcgctgcct gtgcgttacg ccgcggttct
     2041 gacggtcatc aacgcgctgg tcgatttcgt cgcgcgtttt ccgaatccgc atcccctgct
     2101 ggtggtggcc gggcaggact ttggtaaagc tctgggcatg ttgttgcgcc cacagctaca
     2161 acaactcccg ttggcagtca ttgacgaagt gattgtccgc gcgggggact atatcgacat
     2221 tggtacgcct ctttttggcg gatcggttgt gccggtgacg gtgaaatcac tcgcatttcc
     2281 ttcctgaata cggtagttat caggtatggt gcttggtgtc tgggtgttag tgggattata
     2341 tccggcagtg tatccggtta aggtgattgg tctggcaaga tgattggatg cttttgtggt
     2401 gaaaaatgtt ttggtgtttt agcataaccc tgaagaaaga ataaaaataa aaaggaaaaa
     2461 acc
//
LOCUS       DAZPEU010000338         2412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPEU010000338 DAZPEU010000000
VERSION     DAZPEU010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.344"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACHU2L_004196"
     CDS             complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACHU2L_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HFR1358789.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="ACHU2L_004197"
     CDS             complement(1207..2196)
                     /locus_tag="ACHU2L_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358790.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2412)
                     /locus_tag="ACHU2L_004198"
     CDS             complement(2280..>2412)
                     /locus_tag="ACHU2L_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358791.1"
                     /translation="REQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          645 a          553 c          577 g          637 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc gg
//
LOCUS       DAZPEU010000339         2401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPEU010000339 DAZPEU010000000
VERSION     DAZPEU010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.345"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            268..411
                     /locus_tag="ACHU2L_004199"
     CDS             268..411
                     /locus_tag="ACHU2L_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR1358792.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            424..633
                     /gene="cspE"
                     /locus_tag="ACHU2L_004200"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             424..633
                     /gene="cspE"
                     /locus_tag="ACHU2L_004200"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1358793.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            799..1608
                     /gene="rlmA"
                     /locus_tag="ACHU2L_004201"
     CDS             799..1608
                     /gene="rlmA"
                     /locus_tag="ACHU2L_004201"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR1358794.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="ACHU2L_004202"
     CDS             complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="ACHU2L_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR1358795.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2168..2290)
                     /locus_tag="ACHU2L_004203"
     CDS             complement(2168..2290)
                     /locus_tag="ACHU2L_004203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358796.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     regulatory      complement(2303..2393)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT          627 a          585 c          594 g          595 t
ORIGIN      
        1 gcactatgta ataacttctc cctctggcct ggaacaactg aacttattga actatgttag
       61 aaactacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg
      121 cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc
      181 tgatcgagat atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taaaagattt
      241 cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt
      301 aaacacgttg acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt
      361 aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt
      421 caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc
      481 attactccgg ctgatggcag caaagatgtg ttcgtacact tctccgctat ccagggtaat
      541 ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa
      601 ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat
      661 acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt
      721 cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag
      781 tatcctgccg gagttgtcat gtcgttttcc tgtccacttt gccatcagcc tctttcgcgt
      841 gaaaaaaaca gctatatctg tccccaacga catcagtttg atatggcgaa agaagggtat
      901 gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg
      961 atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt
     1021 gtcgcccaac tgcgggaacg gcttgatgaa aaggccacgg cggtgctgga tattggctgt
     1081 ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggc
     1141 ctggatgtct cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactctt
     1201 tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt
     1261 atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc
     1321 attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa
     1381 gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg
     1441 gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg
     1501 ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc
     1561 gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc
     1621 gtccagagga tctggacgcc gatgccgatc agcaccagcc cgccgagaat ttccgctttt
     1681 ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgtcga cataatcaag
     1741 gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgaaggaa agcaagacca
     1801 acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa
     1861 ccgtgccgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc
     1921 attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtgattcca ttcaaggaca
     1981 aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca
     2041 ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag gggggcacct
     2101 ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca
     2161 gtgatattca tgacaacgtc ctgaccgggg tatccataaa acacatcatc gcccccagca
     2221 aacatgcata catgaacaac aagccgcgat gggttttcgc gttcatgcag gcatagcgat
     2281 tgatgtgcat atggtctcgc ctgactgaaa gtcttcagtc cgtacgtgcc acgttttgca
     2341 acgagtatgt tgacacgtac atttccggaa tctggaaatc ggctactccc caaatgaggg
     2401 c
//
LOCUS       DAZPEU010000340         2390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPEU010000340 DAZPEU010000000
VERSION     DAZPEU010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.346"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..837
                     /gene="spa33"
                     /locus_tag="ACHU2L_004204"
                     /gene_synonym="spaO"
     CDS             <1..837
                     /gene="spa33"
                     /locus_tag="ACHU2L_004204"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR1358797.1"
                     /translation="LYAKQLCERFSIQTFKNKFTGSESLVTLTSVCGDWVIRIDTLSF
                     LKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITFRITNEIQYATTGSHL
                     CCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLATLKRIRTGDIIIVQK
                     LYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLALFNYDDINVKVDFILL
                     EKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELVSIEDGYGIEISSWMV
                     KE"
     gene            842..1492
                     /gene="spa24"
                     /locus_tag="ACHU2L_004205"
                     /gene_synonym="spaP"
     CDS             842..1492
                     /gene="spa24"
                     /locus_tag="ACHU2L_004205"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR1358798.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            1507..1767
                     /gene="spaQ"
                     /locus_tag="ACHU2L_004206"
     CDS             1507..1767
                     /gene="spaQ"
                     /locus_tag="ACHU2L_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR1358799.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            1767..>2390
                     /gene="spa29"
                     /locus_tag="ACHU2L_004207"
                     /gene_synonym="spaR"
     CDS             1767..>2390
                     /gene="spa29"
                     /locus_tag="ACHU2L_004207"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR1358800.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAP"
BASE COUNT          650 a          355 c          450 g          935 t
ORIGIN      
        1 ttatatgcaa agcaactctg cgagcgtttt tcgattcaga cattcaaaaa taaatttaca
       61 ggcagtgaaa gtttagtcac tcttacttcc gtgtgtgggg attgggtaat tcgtattgat
      121 acattatctt ttttgaaaaa aaaatacgag gtattttcag gattttctac acaagaatct
      181 ttactgcatt tatcaaaatg tgtctttata gagtcgtcat ctgtattttc gattccagaa
      241 ctgtctgata agattacttt ccggatcacg aatgaaatcc agtatgcaac tactgggagt
      301 catttatgct gtttttcctc ttctttaggt attatttatt ttgacaagat gccggtatta
      361 cgtaatcaag tttctcttga ctcattgcat catcttttag agttttgctt aggttcatct
      421 aatgtaaggc tggctacttt aaaaagaatt cgcactggtg atataatcat agttcagaaa
      481 ctttataatt tattattgtg taatcaagtt attattgggg attatattgt gaatgataat
      541 aatgaggcaa aaattaatct gtcagaaagt aatggtgagt cagaacacac agaagtttct
      601 ttggcattat tcaattatga tgatatcaat gtaaaagtgg actttattct tttagaaaaa
      661 aatatgacaa tcaatgaact aaaaatgtat gtagaaaacg aattattcaa gtttcccgat
      721 gacatagtta aacatgtaaa tattaaagta aatggttctt tggttgggca tggggaactt
      781 gtttctattg aggatggtta tggtatcgag attagttctt ggatggtaaa ggagtaatct
      841 catgctgagt gacatgtccc tcatcgctac actttctttt tttacgctat taccattttt
      901 agttgctgct ggtacctgtt atataaagtt ttctattgtt tttgtaatgg tacgaaatgc
      961 acttggtttg cagcaggttc catcaaatat gactctcaat ggtattgcat tgattatggc
     1021 tttgttcgtg atgaaaccca ttattgaagc tggttatgaa aattatctaa atggccctca
     1081 aaaatttgat acaatatctg atattgtccg tttttctgat tctggcttga tggaatataa
     1141 gcaatacttg aaaaagcata cagatcttga gttagcacgt tttttccaaa ggtctgaaga
     1201 agaaaatgct gatttaaaga gtgcagagaa taatgattat tcattgtttt ccctcttgcc
     1261 tgcatatgcg ctaagtgaaa tcaaagatgc gttcaaaata ggtttttatt tatatttgcc
     1321 atttgttgtt gttgatcttg ttatttcaag cattttactt gcacttggta tgatgatgat
     1381 gagtccaatc acaatatcag taccaataaa acttgtatta tttgttgcgc ttgatgggtg
     1441 gggtatcctg tctaaggcct tgattgaaca atatatcaat attcctgctt agatgatgga
     1501 ggtaatgtgt ctgatatagt ttatatgggt aataaggctc tttatttaat ccttatcttt
     1561 tccttatggc cagtaggtat agctacggtt attggattat ctattggttt gttacagaca
     1621 gtgactcaac tccaagagca gacacttcct tttggtataa agcttatagg tgtctcaata
     1681 tctttgctac ttctttctgg atggtatggt gaggttttat tgtctttttg tcatgaaata
     1741 atgtttttaa ttaagagtgg ggtttgatgg acatttcaag ctggttcgaa agtattcatg
     1801 tgtttttaat actcctgaac ggcgtttttt ttagattggc tccattattt ttctttcttc
     1861 catttttaaa taacggtata atttctccat ctattagaat acctgtgatt tttcttgttg
     1921 catcaggatt aattacttct ggtaaggtag acataggttc ttctgttttt gaacatgttt
     1981 atttccttat gttcaaggaa ataattgttg gcctccttct ctctttttgc ttgtctcttc
     2041 ccttttggat atttcatgct gttggtagca ttattgacaa ccagcgtggg gcaacgctta
     2101 gtagttcaat tgatcctgcc aatggtgttg atacgtctga gttggcaaaa tttttcaatc
     2161 ttttttctgc agttgtattt ctatacagtg gtggtatggt ctttatttta gaatccatac
     2221 aattgtctta taatatatgc ccgttatttt ctcaatgttc tttccgtgtc tcaaatatct
     2281 taacatttct gactttattg gcaagtcagg ctgttatttt agccagtcct gttatgatag
     2341 tattgttact atcagaagta ttacttggtg tattatcgag atttgctccg
//
LOCUS       DAZPEU010000341         2342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPEU010000341 DAZPEU010000000
VERSION     DAZPEU010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.347"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHU2L_004208"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHU2L_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR1358801.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHU2L_004209"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHU2L_004209"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR1358802.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACHU2L_004210"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACHU2L_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR1358803.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          527 a          603 c          550 g          662 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 at
//
LOCUS       DAZPEU010000342         2328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPEU010000342 DAZPEU010000000
VERSION     DAZPEU010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.348"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1013
                     /locus_tag="ACHU2L_004211"
     CDS             <1..1013
                     /locus_tag="ACHU2L_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1358804.1"
                     /translation="GSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGFYLTCVVAVMF
                     VFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQAKGIEPLSIE
                     KLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDIISKRRESLEN
                     QWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVFPSDYISDCAN
                     NRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKAHELFESEEDY
                     QKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIRNVPWGASSVK
                     YS"
     gene            complement(1130..>2328)
                     /gene="gdhA"
                     /locus_tag="ACHU2L_004212"
     CDS             complement(1130..>2328)
                     /gene="gdhA"
                     /locus_tag="ACHU2L_004212"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR1358805.1"
                     /translation="LLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKG
                     GMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLM
                     TELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPE
                     ATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGT
                     VVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQN
                     ELDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLE
                     MAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAM
                     LAQGVI"
BASE COUNT          598 a          570 c          564 g          596 t
ORIGIN      
        1 ctgggagcga gaagtattct tttaatggct ggggaatatt taatagtaat tttgaacggg
       61 tgattaaaga aaataaggcc atgctgcttt gtaagtgggg gttttattta acatgtgttg
      121 tcgctgtaat gtttgtattc gcagcgataa catccaacgg tttgaatgaa agaggcctga
      181 ttaccgcggg atgctctttt ctttatctat taattatgat ggggcttatt gttcgggccg
      241 gttttaaagc aaaaaaagaa caactgcatt attatcaggc taaaggtatt gagccgctca
      301 gtatcgagaa gttacaggcg ctacaattga tcgcacctta tcgattctat cataagcaat
      361 ggtctgaaac gctagagttc tggccgcgaa agcctgaacc tggcaaagat accttccaat
      421 atcatgtgct tccttttgac tcgatcgata tcatcagtaa aagacgcgag tctttagaga
      481 atcaatgggg tatcgaagat agcgaaagtt attgtgcctt aatggagcat tttctttctg
      541 gcgaccatgg agccaatacc tttaaagcaa acatggagga agccccagag caggttatcg
      601 ccttgttgaa taaatttgct gtttttccct cagactatat ctctgattgc gctaataatc
      661 gctccggtaa atcctcggcg aagctaatat gggcggcgga attatcatgg atgatctcga
      721 tatcaagcac agcttttcaa aacgggacaa ttgaagaaga actggcctgg cattatataa
      781 tgcttgcttc tcgaaaggcg cacgagttgt tcgaaagcga agaagattat caaaaaaata
      841 gtctaatggg atttctttac tggcatatct gctgctatcg cagaaagtta acggatgctg
      901 aacttgaagc gtgttatcgt tacgacaagc agttttggga gcactacagt aagaaatgcc
      961 gttggcccat aagaaatgtt ccctggggag catcatccgt taaatactca taaacgcctg
     1021 aaattttgcc gggggcgctg cgcttgaccg gcctacatta gagcgtaact gcaatgaatt
     1081 gtgcccatct gtaggcctga taagcgtagc gccatcaggc atttacaact taaatcacac
     1141 cctgcgccag catcgcatcg gcaaccttca caaaaccggc aatgttcgcg ccctgcacgt
     1201 agttggtttg ctcaccttca ccaccatgct caacacaggc atggtggata tccagcatga
     1261 tgtgatgcaa acgtgcgtca actttctcgg ctttccagcc caggcgcgca gcgttttgtg
     1321 ccatttccag gcccgacgtt gctacaccgc cagcattagc cgctttaccc ggtgcaaaca
     1381 gtacgcctgc ctgctggaac agttcagtcg cttcgatggt ggtcggcata tatgcccctt
     1441 cagcgacggc tttaacgcca ttggcgataa gctgatgcgc ggcgtcaaca tccagttcgt
     1501 tctgggtggc gcaaggcagg gcgatatcaa ccggtacaga ccacggctgt tggccttcga
     1561 gatagaccag accaaattct ttggcgtaat ctgccgctcg accatcgcgg ctggctttga
     1621 tttcgataag acgtgccagt ttctctttcg tgaatccgct ttcatcaact acagtgccgc
     1681 tggagtctga cgcagtgatc acacgagcac caaattccat cgctttttcg atagcgtact
     1741 gggcgacgtt gccggagcca gaaacggaaa cgctcatccc ttcaaaaccc ataccgtggc
     1801 gttttaacat tgcttctgtg aaataaacca gaccgtagcc ggtagcttcc gggcgaataa
     1861 gactaccgcc aaatgaaagg cccttaccgg tgaagacgca ggcggtattg ttggagagct
     1921 ttttcatcat ccccgccata aagccgactt cacgaccacc aacgccgata tcacctgccg
     1981 gaacgtcggt atccgcgccc aggtggcgat acagttcagt catcagcgtc tggcaaaaac
     2041 gcatcacttt accttcgctt tttcctttcg gatcgaaatc gctgccgcct ttaccaccgc
     2101 ccatcggcag ggtagtcagg gcatttttga aggtttgttc aaagccgagg aatttgagaa
     2161 tggaaaggtt aactgacgga tggaagcgca taccgccttt gtacgggccg atggcagagc
     2221 tgaactgcac acgccatgca cggttgacct gtatctggtt gcgatcatca acccatacca
     2281 cgcgaaactg gatcacgcgc tccggttcaa ccagacgctc cagtaatg
//
LOCUS       DAZPEU010000343         2325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPEU010000343 DAZPEU010000000
VERSION     DAZPEU010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.349"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACHU2L_004213"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACHU2L_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1358806.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHU2L_004214"
     CDS             282..452
                     /locus_tag="ACHU2L_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR1358807.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHU2L_004215"
     CDS             527..802
                     /locus_tag="ACHU2L_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358808.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACHU2L_004216"
     CDS             902..>2325
                     /locus_tag="ACHU2L_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR1358809.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZPEU010000344         2296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPEU010000344 DAZPEU010000000
VERSION     DAZPEU010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.351"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(155..250)
                     /locus_tag="ACHU2L_004217"
                     /pseudo
     CDS             complement(155..250)
                     /locus_tag="ACHU2L_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(286..591)
                     /gene="ccdB"
                     /locus_tag="ACHU2L_004218"
     CDS             complement(286..591)
                     /gene="ccdB"
                     /locus_tag="ACHU2L_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR1358810.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(593..811)
                     /gene="ccdA"
                     /locus_tag="ACHU2L_004219"
     CDS             complement(593..811)
                     /gene="ccdA"
                     /locus_tag="ACHU2L_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR1358811.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(1347..2296)
                     /locus_tag="ACHU2L_004220"
                     /pseudo
     CDS             complement(1347..2296)
                     /locus_tag="ACHU2L_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          538 a          622 c          558 g          578 t
ORIGIN      
        1 taataaggat aaacaaaatt ggctgtaaaa aaacgatact gtttcagtta accgtgaatg
       61 gcctgtttgc accgtattgt tcttgttttt cggttagtta aacatcgtac ttcatatttg
      121 aacggtaagc ggctcgtcag aaccgtattg atatttactg agaggtcaga tcaactttcc
      181 aaggcaacag atcgcgtacc cggtttgccg gccagtcctg gatatgctca atgacgtaac
      241 gcagccattt tgtcaacgcc acgatttttg accctagcct gacatttata ttccccagaa
      301 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
      361 cccgataacg aagatcggca cactggccat atcagtggtc atcatgcgcc agctttcatc
      421 cccgacatgc accaccgggt aaagctcacg ggagacttta tctgacagca ggcgggcact
      481 ggccaggggg atcaccatcc gtcgtccggg cgtgtcaata atgtcactct gtacatccac
      541 aaacagacga taacgactct ctcttttata ggcgtaaacc ttaaactgca tttcaccagt
      601 ccctgttctc atcggcaaaa gagccgttca tttcaataaa acgggcgatc tcagccatcc
      661 cctgctgatt ttcagcctgc cagcgttccg cacgcagacg gcgggcctct ttttgcatgg
      721 ccgtatttac caggcctgag atattcacgt ttgcggattt aagtaactga tagctgtcac
      781 tgtctatagt gacagtaata cgctgcttca tgataagccc tcaaaaacat acttatgata
      841 tgtatatgag tatatattaa tggtacaaac catcaatatc cataaaaagt acaccattaa
      901 ttcgctataa gtttttaata caattttttc tgctgcgatt tttttatact ataattagca
      961 tacttataac accgtaagta aattacatga aagcaggcac agcgtgatcc gatttttctg
     1021 cgtataacga ttttccggaa gtgtaatacg gttgcgcccc gggccgggca cccttttctg
     1081 ttgttgtgta cgggcccaca gagaatggta ccggaggcgg gctcagaagt actgagtcgg
     1141 gtgatcgcgt caggccgcga agtactgaca cgggtattgg tgactggtga tgaagtactg
     1201 ccccgggtat gatttcaggc tgattcaggt tctgattctc tgtcagtccg cgtggttgct
     1261 ggcctggctg atttcctgtt gtcaggcgtt caacaaccga ctgcaggtta tagcccggtt
     1321 cacgaacacc tgacggcagg tatcactcac ccggtgaagg taaatccctc aagaggcagc
     1381 aactctgcca gtcgctcctc cggccattcc ggcagacgcg tcaggacgtc cgtcagccag
     1441 gcatgtggct ccagaccatt acgttttgct gtttccagaa ggctcattat ctgtgccgcc
     1501 cggtttccgg ccatcagtga accggcgaac agccacgctt tccgtcccat aacaaccggg
     1561 cgaatggcac gctcgcagat attgttgtcc aggggcacag ctccgtcacc caggaagcag
     1621 ctcagttcat cgcgccggtt cagaatatag ttaatcgcct tatttaatgg cccgtcgggc
     1681 ggacagcacg gctcctgctc ttcaagccag gcaaacaggt cattcactat cggccgggag
     1741 taccgttatc gccactgccg tattttttcc acagggcgct cacggatgag tttctcaatg
     1801 cggtaaagac ccgcgatttt tctcagctcc acgcctgcac ggggctccct gctggcctta
     1861 tacagatttg cgaagccgcg ccctgcgtga ctccagcaac aggctgatgt gatgccgggg
     1921 tgattatccg ccagagattt gtacacgctg tatccgtcac tcaccagcgt actgccacac
     1981 cagcactgca gccaggtctc agggtatctg agggcccgtc cggtctgcga gtcaaaacag
     2041 acgaccggcg gaccgcttct ttcaccgctg acgtaagcac acagccagcc ggagcaggat
     2101 ttaccgcctt tccgggtatc aaggagcctc agggacgttt catctgcatg aataacatcc
     2161 cgtgtcagaa gctcatggcg tagcagtttc gccagcgggg ccagtgcagc tccggcaaca
     2221 ccgaccatat ccgccattgt actgacgggg agctccacac ccattcggga gaaaatgtgc
     2281 tgctggcgat acaggg
//
LOCUS       DAZPEU010000345         2295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPEU010000345 DAZPEU010000000
VERSION     DAZPEU010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.352"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..331)
                     /locus_tag="ACHU2L_004221"
     CDS             complement(<1..331)
                     /locus_tag="ACHU2L_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358812.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            493..549
                     /gene="ysgD"
                     /locus_tag="ACHU2L_004222"
     CDS             493..549
                     /gene="ysgD"
                     /locus_tag="ACHU2L_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR1358813.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="ACHU2L_004223"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="ACHU2L_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR1358814.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="ACHU2L_004224"
     CDS             complement(1692..2072)
                     /locus_tag="ACHU2L_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR1358815.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..>2295)
                     /gene="yigG"
                     /locus_tag="ACHU2L_004225"
     CDS             complement(2086..>2295)
                     /gene="yigG"
                     /locus_tag="ACHU2L_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFR1358816.1"
                     /translation="NMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNG
                     SMIFLICYFSTFSMLMLAPTDSSKQ"
BASE COUNT          605 a          537 c          529 g          624 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgtt
//
LOCUS       DAZPEU010000346         2287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPEU010000346 DAZPEU010000000
VERSION     DAZPEU010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.353"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(105..254)
                     /locus_tag="ACHU2L_004226"
                     /pseudo
     CDS             complement(105..254)
                     /locus_tag="ACHU2L_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            330..2027
                     /gene="ospD3"
                     /locus_tag="ACHU2L_004227"
     CDS             330..2027
                     /gene="ospD3"
                     /locus_tag="ACHU2L_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR1358817.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            2144..2263
                     /locus_tag="ACHU2L_004228"
                     /pseudo
     CDS             2144..2263
                     /locus_tag="ACHU2L_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          764 a          423 c          449 g          651 t
ORIGIN      
        1 atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg atatattgtt
       61 tattgtcagt atggctcaat gtgataatag ttggaaagtt tgatgggttt cgccccgttg
      121 tagcggtagt cgaactggaa ggtcttcagg cactgcttac agcgatagag cagcccccct
      181 gaactggaat ggccgttccg gatacccccc tgagtttcag agtaacggtg acaaaccaca
      241 tcaatctttg ccatcaatca tccaaagggc aaagagtaca acaacactaa gtctgcgtca
      301 caacccatca atgaaaggaa tatatacata tgccatcagt aaatttaatc ccatcaagga
      361 aaatatgttt gcaaaatatg ataaataaag acaacgtctc tgttgagaca atccagtctc
      421 tattgcactc aaaacaattg ccatattttt ctgacaagag gagtttttta ttaaatctaa
      481 attgccaagt taccgatcac tctggaagac ttattgtctg tcgacattta gcttcctact
      541 ggatagcaca gtttaacaaa agtagtggtc acgtggatta tcatcacttt gcttttccgg
      601 atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa ggctattaat gtgcctgcta
      661 ttatttattt tgttgaaaac ggttcatggg gagatattat tttttatatt ttcaatgaaa
      721 tgatttttca ttccgaaaaa agcagagcac tagaaataag tacatcaaat cacaatatgg
      781 cattaggctt gaagattaaa gaaactaaaa atggggggga ttttgtcatt cagctttatg
      841 atcccaacca tacagcaact catttacgag cagagtttaa caaatttaac ttagctaaaa
      901 taaaaaaact gactgtagat aattttcttg atgaaaaaca tcagaaatgt tatggtctta
      961 tatccgacgg tatgtctata tttgtggaca gacatactcc aacaagcatg tcctccataa
     1021 tcagatggcc tgataattta cttcacccca aagttattta tcacgcgatg cgtatgggat
     1081 tgactgagct aatccaaaaa gtaacaagag tcgtacaact atctgacctt tcagacaata
     1141 cgttagaatt acttttggca gccaaaaatg acgatggttt gtcaggattg cttttagctt
     1201 tacaaaatgg gcattcagat acaatcttag catacggaga actcttggaa acttctggac
     1261 ttaaccttga taaaacggta gaactactaa ctgcggaagg aatgggagga cgaatatcgg
     1321 gtttatccca agcacttcaa aatgggcatg cagaaactat caaaacatac ggaaggcttc
     1381 tcaagaagag agcaataaat atcgaataca ataagctgaa aaatttgctg accgcttatt
     1441 attatgatga agtacacaga cagatacccg gactaatgtt tgctcttcaa aatggacatg
     1501 cagatgctat acgcgcatac ggtgagctca ttcttagccc ccctctcctc aactcagagg
     1561 atattgtaaa tttgctggcc tcaaggagat atgacaatgt tcccggactt ctgttagcat
     1621 tgaataatgg acaggctgat gcaatcttag cttatggtga tatcttgaat gaggcaaaac
     1681 ttaacttgga taaaaaagca gagctgttag aagcgaaaga ttctaatggt ttatctggat
     1741 tgtttgtagc cttgcataat ggatgtgtag aaacaattat tgcttatggg aaaatacttc
     1801 acactgcaga ccttactcca catcaggcat caaaattact ggcagcagaa ggcccaaatg
     1861 gggtatctgg attaattata gcttttcaaa ataggaattt tgaggcaata aaaacttata
     1921 tggaaataat aaaaaatgaa aatattacac ctgaagaaat agcagaacac ttggacaaaa
     1981 aaaatggaag tgattttcta gaaattatga agaatataaa aagctgaata ttatctcgtg
     2041 gtgatacact gaatttgagg taaccggctc atttaaaccg tctggtctgt tacctccggt
     2101 tccacaaaaa taatgtccat cattttgaag tggacactat cgtatggaat accggacctg
     2161 gatcactgaa gctttacgcc ttcatttcga agaacattta ccacgggttg tggccggacg
     2221 tcgcctgggc gtaccaaaat caactgtctg tggtatgttc gtgtgaaccg cccccggtat
     2281 cccgctc
//
LOCUS       DAZPEU010000347         2279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPEU010000347 DAZPEU010000000
VERSION     DAZPEU010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.354"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(562..2166)
                     /locus_tag="ACHU2L_004229"
     CDS             complement(562..2166)
                     /locus_tag="ACHU2L_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1358818.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          590 a          553 c          504 g          632 t
ORIGIN      
        1 ttaactttaa gttaagcccc tgtagggact agagaccata attaagatga ttgtgtctta
       61 ttgctattca cttatcgact gacccagagc acacaggaag cattcacttt aagtagaacg
      121 cttggtgtct gtaattggtc atcccgcccc tgaaaggatg cgataagtta cggttagttg
      181 tcctagaaga gaaaactttt tgtgtccctt ctccctatag tgagtcgtat taattatcct
      241 tgacaagtag agaaaaccat gagtcaaaaa catgaccaca caggtctgta gtaagtgtca
      301 tcctagaaga gaaaactttt tgtgtccctt ctccctatag tgagtcgtat taattacccc
      361 ttgacaagca gagaaaacta tgagtcaaaa acatgaccgc acaggtctgt agtaagtgtc
      421 atcctagaag agaaaacttt ttgtgtccct tctccctata gtgagtcgta ttaattatcc
      481 cttgacaagt agagaaaact atgagtcaaa aacatgacca cacaggtctg tagtaagtgt
      541 catcctagac tatggtctcc tctaggtctc cttataaaca tcagccatca cattgaccag
      601 cctatcaacc tcgacctctt tagcataacg gtcgtacgta atagaccctg aactatgccc
      661 catgatcgcc tgtgcatacg ccaactcgac cccatgttgt tttaactggg ccgcaactgt
      721 atgtctaaac gagtgaaacg ctaagccgct acggtctacg aggactttag gcaacaacgt
      781 ccgattaaac catttactca cctgctcacc gtaaccgttc ttcatgagac gcagaccatc
      841 gaacaccata gcgtcatcac cgtcagcgcc tctacgggtc tctacaagcg ccataaagtc
      901 agccaatata aaaccatagg ccccatcgac cagaggcaca cagcggtcac tatgagcgtt
      961 cttgatgctc ttaccgggta gactactatc gtcttcattg atatgaatat aaacggttcc
     1021 cgcctcagtg gtcctgacgt ctttgacctg tagttgggct atttcattga gtctcgcccc
     1081 agtgatagca gccaaggcgg taacataata atgatacggc ttaccagagg tcttctgaga
     1141 gtatgcctta gctgcgacca gtagttgccc cacctgctcc gtagagaagg cgttacgggc
     1201 ccctgaggct ttacgctgag ggactttaag ctccagaccc tcagtcatgt tctttttaat
     1261 caagtcattg cgcacagccc acctaaagac agcagccatc ttaataagat acttgttgtt
     1321 gatggtaacg acatccagac aatctgtctt atcttcacgg ctcaacaggt cagcaagtgg
     1381 gacatccttg aagcgctgct tacggttcct aggtaactgc tggagcacat cacggaccct
     1441 aagcatgtca gccctgttag cgtctgcatt gagacccaga tggtcgaata tttcgattaa
     1501 cgcagcgtgt gacgccttgt tttcccttaa ggttgcaggc ttccagttct gatagttctc
     1561 cgcttcgtat tgttcaaaga ggctcgctat ggtaatgcta ggcgtaactt ctggttttac
     1621 ttctggagca ttaacagata gggacacaga gtctgcttgg ttatcttgcc tatcaatatc
     1681 attaaaaaga tcgacgtaat ctatcagagg ctgactgtct cccgcttcga tacgcctcat
     1741 acaggctttt agcaccttca gggcctcatt gatgtaacga tgctggtcaa tgcttagcgg
     1801 ctcactagcc agagcatcgg taaggctctc accaagttct ccgtattggt cccggtagat
     1861 atccctcatg tcaggctcaa agagatctga acggcccata gacagttcac actcagcgat
     1921 gtccttaagg tgttccctca tttcctcata ggtagcgttg ggtctgtctg cgtgtattgc
     1981 cttgagtgct gcctttatgt gccttgagta agccatagcg gtgcgtctat ccttggtccg
     2041 taatgatatt gaggtcattc tatcattgtt tgattgtctg accctaagac ggaagtagta
     2101 gacgccgtta cgctggtaga gatagtagac tgctggaagt ttgtgactac tggatagctg
     2161 aatcatatgt ctgcatgccc tgagagggtt acacagtggg ttacagtaag taactctgca
     2221 aggaggctta aagccgtatc ccttaggatt cagtaggttg gagtacttta agaaaggtc
//
LOCUS       DAZPEU010000348         2273 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPEU010000348 DAZPEU010000000
VERSION     DAZPEU010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.355"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            308..1498
                     /locus_tag="ACHU2L_004230"
     CDS             308..1498
                     /locus_tag="ACHU2L_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFR1358819.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(1888..2273)
                     /gene="gtrII"
                     /locus_tag="ACHU2L_004231"
                     /pseudo
     CDS             complement(1888..2273)
                     /gene="gtrII"
                     /locus_tag="ACHU2L_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
BASE COUNT          636 a          461 c          501 g          675 t
ORIGIN      
        1 gtaccaccga tagtatcgat atcaaatcag cctgcggccg cgataacttt cgtaacattc
       61 tgccagcggc tttgttgaat aaatcaggtt caagataatc gtctctgcat caggttagag
      121 tttcaggcaa tacatcctct ttctggcata cctgccactc tcgcaccatg gtcatagcct
      181 ttacgatctg catactcacc agaccagagc ccgctccctt ttaggctgag gaatccccag
      241 aaaagaagac aggcatagag ttttgtgatc tactgattgc gctaacaaat tcaatgagat
      301 cacctctatg cctgcccgta aagtatgcca gacttttttc cgtaatgctt tagcttccac
      361 gcatcagtat cgacaaaatg ccattattga ttctgctgct gctttagtcg gtggcgcatc
      421 tctttctctt accagcattg gccggcattt acccggtcct gctcgcgtga aagataaaat
      481 aaaacgcgtt gactgtctgt tgggtaataa acgacttcat aatgatatcc ctctgatatt
      541 caaaaatatt acatccatga tgacaaataa actgtcgtgg tgtgttatcg ctgttgactg
      601 gagcggttac ccttttcagg aatatcatgt ccttcgtgcc agcctcctct gcgacggtcg
      661 ctctatcccc ctgatgagtc aggtcttccc gtcaaaaaaa aataacgagg cggtggaaat
      721 cgcttttctg gatgcgcttt ccggggccat ttctccccgg actcgtgttg ttattgttac
      781 tgatgctggt tttcaaagtg catggttccg tcatatcaaa tcacagggat gggattttat
      841 tggtcgtatc aggggggtag tgaagttcag gctggatagt gacaaagata agtggctgga
      901 cataaaaatg tgcaggggga gttcagaggc aaaatacctg gggacgggaa ccctggcccg
      961 gaaaaaacgc tcgcagtgtg aaggacattt ttatctttat aaacattcac ctaaaggcag
     1021 aaaaagcagg cgtgccagag ggaggccggg cttaccaaca acggaaaaag aacaaaaagc
     1081 cgcaggcaga gaaccatggt taatattcag caatacagct gagtttaatg ccaaaaaaat
     1141 catgaaactg tacagtcgaa gaatgcagat agagcaaaac tttcgggatg aaaaaagtga
     1201 acgctttggt tttggccttc gggcgagcag aagtcggagg ggagagcgtt ttctggtatt
     1261 aagtctactg gttacactgg ccagtatcgt actctggcta cttggttatc attttgaaaa
     1321 taaaggattt catttaaagt atcaggctaa tagtctcaaa aaacgaagag tattgtcttt
     1381 tctgacgctg gctgaaaacg tcctgagatt taaccctgaa cttctacgac gagcacaacc
     1441 tgaaaagata ttgtgccggt tggccagcac ttaccgaagt atggtgctgg cttactgatg
     1501 aaaattttct ggggatccct cagcctttcg gggaggaatg agggctctga tattcttttg
     1561 ccgtagttca tcgtgaaacc gtcggatgtc atttggcctg agtgacccat gcctcccaaa
     1621 agcagagtcg ctagtgttat gtaattaggc ggttaaactt caattctcag tgttaagttt
     1681 gggtaaacgc ttgtaaatgg tattgctaat tttttaattg actaatatta gaaacggtta
     1741 gtgtgattgt atatagatat ttgagtgtaa ggtatttaaa ggggcattca gcccctccgt
     1801 ctatttgtga ttgcatccat tgatgtgtgt attattttga gcttgaaaaa gggaggcgct
     1861 ctttaataat aaataaatct atcaaagcta aatattaaat ggaagccacc cttttttctc
     1921 tgatagtttt attatcacca aaccatttat ttcagctatc gaatctggat tcggccagga
     1981 aggtaaactc ttcacaattg gataaatctt ctctatttgt tctttatttg ctggtttgca
     2041 attacagatt ccatagtaat tcataaatgc aaccatccta aaatagttgc caccatccca
     2101 ccaaaaaaaa gattttccaa aagtgtctgc gctcatggag agcatatttc ctgagtcgtt
     2161 tgaaccatat atatatattt tccgttaagg gaaatgcctt ttgtttgaga tgtgtatgat
     2221 atttctttag ctaaagagat atctttctgt cttgcgagag tatcattcaa aaa
//
LOCUS       DAZPEU010000349         2260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPEU010000349 DAZPEU010000000
VERSION     DAZPEU010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.356"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHU2L_004232"
     CDS             complement(<1..242)
                     /locus_tag="ACHU2L_004232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358820.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHARPELRA"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="ACHU2L_004233"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="ACHU2L_004233"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1358821.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          559 c          668 g          459 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc
//
LOCUS       DAZPEU010000350         2257 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPEU010000350 DAZPEU010000000
VERSION     DAZPEU010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.357"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..82
                     /locus_tag="ACHU2L_004234"
     CDS             <1..82
                     /locus_tag="ACHU2L_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1358822.1"
                     /translation="IRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(171..683)
                     /locus_tag="ACHU2L_004235"
     CDS             complement(171..683)
                     /locus_tag="ACHU2L_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR1358823.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            878..1642
                     /locus_tag="ACHU2L_004236"
     CDS             878..1642
                     /locus_tag="ACHU2L_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358824.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1593..>2257
                     /locus_tag="ACHU2L_004237"
     CDS             1593..>2257
                     /locus_tag="ACHU2L_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR1358825.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          646 a          492 c          570 g          549 t
ORIGIN      
        1 tatccgggac ggaatcatca gcgaccagac agcattgaga atgcttcagg agtacatcag
       61 gatccaatgc cttggggggt agcggtaatt ttactcatta tccttcaaat cagattctgt
      121 tgtcagagga atgggggaag ctggattcgc aagagctttt tatgggcagt ttagctaaaa
      181 aaaggcaaca cgaagctaaa actaccaaca taaagtgctg accagctagc agctgaaatc
      241 agactcactg tatacacttt cactggatga agttgcaaca ttcctgccac caaaggagca
      301 atgtaacgca gcacggcgat aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc
      361 aactgaaggc gaacccgttc tattgtattg gaacggctcg atattataca tgcaagttga
      421 gggatatgcc caattattat accaagatgg taattaacga tagtaccgca ccatgcgcca
      481 ctcatgaccg tgattccagc ttcccatggt gaaagagtcg tcttgctgac tgtaattaca
      541 gtggccatca taattgatgc tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg
      601 aacgctatta taaaaagaag cccccatagg cgatatggat gaagtacaaa atagttcatc
      661 aaagcattta tccactccat caatgtcttt ccttctgtga atttttcttt gctatttaac
      721 cgtgatacga cttaatttag gcttaaacac cttacattta caaggtactc ctgtgggggg
      781 ccctgccacg gagcgtcgca ggagtgagtg atgaggaaga attaaagcaa cctattgtga
      841 caggcagcga taacagtaaa tcacaggaag cataatcatg gcaaaaccgg actgggaggc
      901 catcgaatcg gcataccggg ccggagtcct tagcctccgt gatataggcg agaaatacgg
      961 cgttacagaa ggggctatca ggaagagggc caaaaagctt gattgggcac gcagtggcgg
     1021 tacgcaggtt tgcaaaaatg gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc
     1081 cattactggc cttacacaaa aaagtacgca actaaaaaca gaatctacac cggatacgaa
     1141 accgatacgc ggaatgcgta ccgatccccc gactaaccca ttccaacccg gtaaccagca
     1201 ggcattaaaa cacggtggtt atgcccgtcg ccttctgctc aaagatgagg tgatagagga
     1261 cgctaaagcg ttgacgctcg aggacgaatt atttcgcctt cgtgctaaca accttgtcgc
     1321 tgcagagaat attggtcggt ggttggtgtc gctggaagat gctaatgggg accaggaaag
     1381 gaagatgctg attgaaaata tcagcgccgc cgagaaagca atgatgcgca atacagttcg
     1441 tattgagtcc atcgttggca cgcttgcgac ggtaggaaaa atatttgctg atacagccta
     1501 ccgcaaggcc gccactgata aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg
     1561 tattgatgat ggcaatggag agcgtgacct caatgacttc tactctgaca tccaaaccga
     1621 cgctgaatcc ggctttacgt agtttctgga ctatgcgggc acgtaacaaa gtgctttatg
     1681 gtggtcggtc atcgtcaaaa tcatgggatg ccgctggcat tgccatattt ctgtcgaata
     1741 aatacaccct gcgtttttgt tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt
     1801 ataccctgct caaaattcag atagacaggt ttggtctgcg gcaccgtttc cgtattctga
     1861 acaacaaaat cattaaccgg gttactggct cggaatttgt tttttatgga ttatggcgca
     1921 acatcgaaga aattaagtca ctggagggga tcgatatgtt gtggttggaa gaagcccacg
     1981 cactgacgga ataccagtgg aaaattctgg agccaacgat ccgtaaagag ggttcggaat
     2041 gctggttcat attcaacccc ggacttgtta ctgatttcgt ctggcgcaac ttcgttgttg
     2101 atccgcccga aggcactctc atccgcaaaa ttaactatga cgaaaatccg tttctgtctg
     2161 acaccatgct taaggttatc gacgcggcgc gacgccgtga tccggatggt tttaaacatg
     2221 tgtatgaggg cgttccggag tctgatgatg atgcggc
//
LOCUS       DAZPEU010000351         2255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPEU010000351 DAZPEU010000000
VERSION     DAZPEU010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.358"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..655)
                     /gene="ipaJ"
                     /locus_tag="ACHU2L_004238"
     CDS             complement(<1..655)
                     /gene="ipaJ"
                     /locus_tag="ACHU2L_004238"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR1358826.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     "
     gene            complement(852..1661)
                     /locus_tag="ACHU2L_004239"
                     /pseudo
     CDS             complement(852..1661)
                     /locus_tag="ACHU2L_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2008..>2255
                     /locus_tag="ACHU2L_004240"
     CDS             2008..>2255
                     /locus_tag="ACHU2L_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1358827.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          643 a          579 c          392 g          641 t
ORIGIN      
        1 gatccatata gcttccatca ggtctacaca acacccagtg gaggccgaca ggaactccaa
       61 taaaggatac agcaacagct tccaaaactc tttggttaga agataacaca tctcgttgat
      121 gtactatatt acatcccatg ccaattaata agtcccttgc atcgggataa ataaaactaa
      181 ttacttgcga aaaaatgtta ctttcttcaa cgacataagc attcaatcca agaagcctcg
      241 tagccatgac tatcttatca ggcatagaat aaccggcatc cgcgatatta tctttgtgta
      301 ttctcggatt ataattacca ctagttatta agtataaatc ccgctcacaa cgattatcca
      361 aatcaagaga actcttcctt gtcatttcgg acatagaccc tttgtattgt ggtattttat
      421 caacccctaa ttcctttgca gcacagagta aactggcagc tccacaagaa ttgtcataca
      481 tctgcttcct cgccaaaaaa caaccttgtt cgtttactct attctcattt accctcacat
      541 cagtctgatg agatatcata tattctcttg gtatagcccc ctgcaataaa accccatata
      601 acataacacc tgtatgaata catccccgtt tgcaaggctt ccgttgttcc gacatcgacg
      661 agtaattgca gcacagtatt gcatccagca atgcgaacta ctatgagaat tagctgaccg
      721 ggtccttatt ccccgaacga actttaaatc caaaacctca tactcttttc cgtcgtcatc
      781 acgtccgtca ccataacaac ctttcagcat ccaccctttt attaagagtt aatgtccatc
      841 agcatcagca attaataccg cctgatactg acatattttt gtcgtattta cgtccataat
      901 cggttattgt gatacaggtc acaccagctt atttgctatt gttacaataa ccaccttggt
      961 tgagagctgt tttttcaact ttgttatcaa ctcccccaac tccttggtca gtaaggctgc
     1021 cgaccgagca cagtgaatag atagtatccg caggtaccac catgcttact taatactcag
     1081 aaggcgaagt gctctccaat taactctgcc gcactatgct tataagctgc ttttcaatgc
     1141 cgtctatcaa catgtccaat tcattcaatc tgttgtactg gtaatccaac gcagtaatga
     1201 tatatgcagg tattctttca tccactccag taacgtttca tgcaaagcat taatttacgc
     1261 gctacggaac ttcaccagtt gactgagcaa acgatgtggg accagtactg cctgcagttc
     1321 ttctattgtt acagcgattt ctttaccaaa cagctgaacc accatcaaga taaagatagc
     1381 ctgaacattc atcgcaccat tttgttacca atggcaaaag atttaacgaa ccgtgcctgc
     1441 atcaggcgga ctttttatcc ctgctttgtt agctccctaa ccttgtacag agagcctcca
     1501 taagctccca tatcaagcag acatggttcg cagttactga catacaccag aaaactctga
     1561 cagcagactc acttatcctc cacttcactc atatccttat tggtgagatg aacctgaatg
     1621 acatgttttg cgacataaac gccaaccagt gtatatttca cctgtagaga ttctacaatc
     1681 tgtagcgtta tgcatctaat gttaggctct atgatgtcag aaatctgcga gactctacac
     1741 cttctgcatt tacctttatt tatcaccatg ctcctcagtt aacggagaac gtgttcattg
     1801 cattttgtca cttacatttc gcctttggta tcggggggat ataggttcca acgttccgca
     1861 tgagaacctg aaccggcttc acgccacctt gataccagat gccgcacagg tagtcaacaa
     1921 gtgtcacgaa cggtgcaata gtgatccaca cccaacgcct gaaatcagat ccagggggta
     1981 atctgctctc ctgattcagg agagcttatg gtcacttttg agacagttat ggaaattaaa
     2041 atcctgcaca agcagggaat gagtagccgg acgattgcca gagaactggg gctctcccgc
     2101 aataccgtta aacgttattt gcaggcaaaa tctgagccgc caaaatatac gccgcgacct
     2161 gctgttgctt cactcctgga tgaataccgg gattatattc gtcaacgcat cgccgatgct
     2221 catccttaca aaatcccggc aacggtaatc gctcg
//
LOCUS       DAZPEU010000352         2228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPEU010000352 DAZPEU010000000
VERSION     DAZPEU010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.359"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..373
                     /gene="tnpA"
                     /locus_tag="ACHU2L_004241"
     CDS             <1..373
                     /gene="tnpA"
                     /locus_tag="ACHU2L_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR1358828.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..720
                     /gene="tnpB"
                     /locus_tag="ACHU2L_004242"
     CDS             373..720
                     /gene="tnpB"
                     /locus_tag="ACHU2L_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1358829.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            740..1969
                     /locus_tag="ACHU2L_004243"
                     /pseudo
     CDS             740..1969
                     /locus_tag="ACHU2L_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1973..2132
                     /locus_tag="ACHU2L_004244"
                     /pseudo
     CDS             1973..2132
                     /locus_tag="ACHU2L_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          540 a          594 c          620 g          474 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctggcat ccgcatataa
      721 cccgttgtaa agtgaggata tggacacctc acttgctcat gagaatgccc gcctgcgggc
      781 actgttgcag acgcaacagg acaccatccg ccagatggcg aaatacaacc gcctgctctc
      841 acagcgggtg gcggcttatg cttccgaaat caaccggctg aaggcgctgg ttgcgaaact
      901 gcagcgcatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcagat
      961 actggaagca caggagcgaa tcagcgcact tcaggaagaa atggcggaaa cgctgggtga
     1021 gcaatatgac ccggtactgc catctcccct gcgccagtct tcagcccgta aaccgttacc
     1081 agcctcactt ccccgtgaaa cccgggtcat ccgtccggaa gaggaatgct gcccggcctg
     1141 tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag cagctggagc ttatcagcag
     1201 cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc tgttgccggt gtgaccatat
     1261 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
     1321 ggcccatgtt gttgctggaa aatatgcaga ctatctgccg ttataccgcc agtcagaaat
     1381 ataccgtcgt cagggcgtgg agctgagccg tgccacactg gggcgctgga caggtgctgt
     1441 tgctgaactg ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa
     1501 agtccatgcc gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac
     1561 tgcccggctg tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa tgcctccggc
     1621 ggtctggttc gcgtactcac cggaccggaa aggtatccat ccacagaatc atctggccgg
     1681 ttacagcgga gtgcttcagg ccgatgctta cggtggttac cgggcgttgt acgaatccgg
     1741 cagaataacg gaagccgcat gtatggctca tgtccggaga aaaatccacg atgtgcatgc
     1801 aagagtgcca accgacatca ccacggaagc cctgcagcgt atcggtgaac tgtatgccat
     1861 cgaggcagaa gtccggggat gttcagcaga acagcgtctg gcggcaagaa aagccagagc
     1921 cgcgccactg atgcagtcac tgtatgactg gatacagcaa cagatgaaaa ttcattcgct
     1981 gaaaatggaa tgtctccacg gagaacacta ttatccatcg ggaaatagtg cgggcaacag
     2041 tgtttagtta tatcgagtgc gattacaatc gctggcgacg gcaccgttgg tgtggtggtc
     2101 tcagtccgga acaatttgaa aaccagagcc tcacttacga ctgtgtccat attatgtggg
     2161 taggatcaat aaagacgttt tatatgtgtc tgcttgcagc cagaaaattt ataccctgca
     2221 ggtatcat
//
LOCUS       DAZPEU010000353         2226 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPEU010000353 DAZPEU010000000
VERSION     DAZPEU010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.360"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHU2L_004245"
     CDS             <1..1224
                     /locus_tag="ACHU2L_004245"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR1358830.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="ACHU2L_004246"
     CDS             1224..1958
                     /locus_tag="ACHU2L_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1358831.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
BASE COUNT          561 a          455 c          565 g          645 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta
     2221 ttgtag
//
LOCUS       DAZPEU010000354         2218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPEU010000354 DAZPEU010000000
VERSION     DAZPEU010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.361"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHU2L_004247"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHU2L_004247"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR1358832.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHU2L_004248"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHU2L_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR1358833.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHU2L_004249"
     CDS             complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHU2L_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR1358834.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAZPEU010000355         2210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPEU010000355 DAZPEU010000000
VERSION     DAZPEU010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.362"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..926)
                     /locus_tag="ACHU2L_004250"
     CDS             complement(<1..926)
                     /locus_tag="ACHU2L_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR1358835.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNG"
     gene            complement(1358..2077)
                     /locus_tag="ACHU2L_004251"
     CDS             complement(1358..2077)
                     /locus_tag="ACHU2L_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR1358836.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          686 a          421 c          383 g          720 t
ORIGIN      
        1 ccgttttgaa gtgctatata aaatgcagga gtaccatcag agcttctcgt ggccagaagg
       61 ttcaccagtt tagattgaga aatttcacca tttttgtatt tttccatcag tataaataat
      121 acttctgtca gattctttgt tatcgcatga tatataactc tggaattgat caattcatct
      181 ggtaaaagtt tcactgtgtt tgttagaggg gaattatgcc tatcttcaaa tattacctgc
      241 ccgggttgct ccagaccata aattttccaa taaaaatcat caaaaaaatc cttagcggat
      301 aattgactca gttcgaaatg gctatcacaa gtaaatacag tacgtttatg agtaacagtt
      361 cgattaggat cgaaaaactg aacaacccat ttttcacttt cttgtacgcg ctttattttc
      421 aaccctaacg ccatttggtg gtttaatgtg catgctgata atgttctgac aaagtctcca
      481 ttttttttca tgttataaaa aaaattagta ataactgttc cccacttttt attttccaca
      541 aaataaatat aagctggtga ttttgaaaaa tcttccgttt tttccaaaca tacatttttt
      601 gcaatagcat caggggtgct aagaagctga tagtttacct tcccatttgt ttccataaaa
      661 ttcatttccc aataggaagc taggtgacgg caaactattt gtcttccata tatatctttt
      721 gccactccat ttatatttac ctccacattt ctcttttctg aaaaataagg tgctgtttta
      781 ttcttaaact gaattagttc actggagttt gagaccctca taatagaact tgttattgtt
      841 ctattatctc tgttaacaga catatctgtt gataacgaat tttttgttga aaatatacta
      901 gatgaaaatg ttttatttaa cggcatagaa gacaaccata gagatgttat aatactatta
      961 gattgcagtt actaatagta aaagcctcct tacaaggagg tagcttattc agaatgctcc
     1021 tataagatag cgagctcgtc aacgatacag atgatctgat tatattgtgc aaaaggagaa
     1081 caggaaaata ggcactggaa aagatgaagg cctgatggga gcattaacta atgcaaaatc
     1141 cgacaggtta atatcccgaa aagcaatcaa acgactcaaa caggagatgc acgctgaaat
     1201 ggtggacaac tgttcggatt tcagatgagt agctcagcag tttatgagcc gaatgctctg
     1261 tcacgggcag gaatcaggct gcatacgcag tgccaaatct aatcgtagtt attctaacca
     1321 gttttcaagc tacaaggtgg tgtagctggc atcttctcta ctctatcatc aaacgataaa
     1381 atggttcctc tcgtaacatt tgctcttgcc tttcacttcc atctcgatcg cttcgtagtt
     1441 cattacgata gctgacatat ttccagtctt caggtctaac atctgacgcc ggatactccc
     1501 cgggagcgat tttgcttctt aatagattag actcgagaca cattataaat tgtcgtattt
     1561 tatgaagtaa taaagcacta tattgcgagc attcctgatc tgattttaca tatagcgtaa
     1621 actgagctcc tatccccaca cgagattgct gagaaactcg attcatatca gttatcttcc
     1681 atttatctat aggactgtcc tctgaaaata ataaaccaga caaaatttgg aatgccagag
     1741 gaacttgctc cctagctata ctaatatgaa acttgtcccc aacaaaatcc cccttactct
     1801 gaggattttc acgacatgca tgaataaaca catctccact atggttcaca atctggaatc
     1861 cttggtacat gacagaaaaa gattgacata tatcagagta agttggcacg ttcgatgcta
     1921 ctttgaaata gtacgctggg tattgccgta tttggttgta caatatattg aagttatttt
     1981 tcaatctttc attcgcagaa agcatctgcg gtatttcaga cctcacaaca ttcaaagaat
     2041 ctaaatttag ttttagacag ggctttttta tgggcataga aaacgtcctc tataaaatag
     2101 atatattatc ttatattgat tgtttatcat cttttataaa acgatacatt atatgtaatg
     2161 tatgtaagcg taagggtcct ctccgaacat ggtgggaagg cttagaacgc
//
LOCUS       DAZPEU010000356         2195 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPEU010000356 DAZPEU010000000
VERSION     DAZPEU010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.363"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            112..852
                     /gene="phoN2"
                     /locus_tag="ACHU2L_004252"
     CDS             112..852
                     /gene="phoN2"
                     /locus_tag="ACHU2L_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR1358837.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            925..1047
                     /locus_tag="ACHU2L_004253"
     CDS             925..1047
                     /locus_tag="ACHU2L_004253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358838.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(1205..2195)
                     /gene="ospC4"
                     /locus_tag="ACHU2L_004254"
                     /pseudo
     CDS             complement(1205..2195)
                     /gene="ospC4"
                     /locus_tag="ACHU2L_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
BASE COUNT          667 a          432 c          385 g          711 t
ORIGIN      
        1 tttttccatc actctgttca aatttttccg catgacttgt gttttttgta atacagctcg
       61 ttttttacat ctgaccaaaa tcatcaatta attatgctaa ggaaataaat tatgaaaacc
      121 aaaaactttc ttcttttttg tattgctaca aatatgattt ttatcccctc agcaaatgct
      181 ctgaaggcag aaggttttct cactcaacaa acttcaccag acagtttgtc aatacttccg
      241 ccgcctccgg cagagaattc agtagtattt caggctgaca aagctcatta tgaattcggc
      301 cgctcgctcc gggatgctaa tcgtgtacgt ctcgctagcg aagatgcata ctacgagaat
      361 tttggtcttg cattttcaga tgcttatggc atggatattt caagggaaaa taccccaatc
      421 ttatatcagt tgttaacaca agtactacag gatagccatg attacgccgt gcgtaacgcc
      481 aaagaatatt ataaaagagt tcgtccattc gttatttata aagacgcaac ctgtacacct
      541 gataaagatg agaaaatggc tatcactggc tcttatccct ctggtcatgc atcctttggt
      601 tgggcagtag cactgatact tgcggagatt aatcctcaac gtaaagcgga aatacttcga
      661 cgtggatatg agtttggaga aagtcgggtc atctgcggtg cgcattggca aagcgatgta
      721 gaggctgggc gtttaatggg agcatcggtt gttgcagtac ttcataatac acctgaattt
      781 accaaaagcc ttagcgaagc caaaaaagag tttgaagaat taaatactcc taccaatgaa
      841 ctgaccccat aaagctggac agcctgtatc aggctatgga gggcccatag acaaatctac
      901 cctatatgag cataggagga gtctatgggc acaccacgtt ttacccctga atttaaggga
      961 ttactggaaa ggctgggaca tatccctccg gcagaagcag aaaaagctta ttatgctgcc
     1021 atcggaaacg atgatctggc aacctgagtt cacagataaa acattctcta ggaaactcgg
     1081 ggcggttccg ttcaccacat gcaatgtggt gttgcagggg aacggtctgc accatcccct
     1141 atgtcgatca atataacaga aatgacaact tcagattcag ggcacaacct aaatatattt
     1201 taggtcacct ctcaaatcgt ttgcctgata ccgctccatt ttttaataaa aaaatcaatc
     1261 atttttgaat ctttgctttt cattgcattg tcaagcatag tgtccccact atttgctttt
     1321 tgaaattttt tgtttacatc aaccaatcca ttattgataa aatattctag cactttatag
     1381 ttggcaccat ctttactaag taagtattca acatcataca gattacaata tctattgttt
     1441 ttcattttct ccatctctgc gacatcttgt tttgtaaaat taaatttaga tagtaaataa
     1501 agtgctatat cagctttttt attgcctaaa gaataagcaa gggcctgatg agccattttt
     1561 ttatcagtca ctttattgtt aatctcttca taattagatg ctttaatcgc atcctccaaa
     1621 gatatgtctc tgagttttat ttttttgaag ttatctgtac ttagcattct tggtatatga
     1681 tactcaagct gaaacacaaa gtttataatt ctatccagac taacaggatc aatatttttg
     1741 ttataacaaa actctctgaa tccttgatct ttactctttc ttataaaatc aataagatgc
     1801 aacccaattc caagcctcat atctttagtg ttaaatattg gaacatcagt tttcccctga
     1861 ttaccatgca catacctgct aacttcatcc acaacctctt taaagttatc taagaaaaat
     1921 gattggtgct catggtaaaa aacaggtgga atagcattat caaagtgatc ggttaatgtc
     1981 atatatccat atggagagtc atgatcaatg atatacgcat tggcgccaaa atctacagta
     2041 tgatgtgttt tatttagtgg gagtttttct tgatgattgg aaatttcgac cccaaaaaaa
     2101 acaaaatcgt ggttagagat ttctttaata tcctcagagt atgtattctt atgtgtttgt
     2161 atattttcag actgaatttt atcaaaggat ttaat
//
LOCUS       DAZPEU010000357         2187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPEU010000357 DAZPEU010000000
VERSION     DAZPEU010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.364"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(220..384)
                     /locus_tag="ACHU2L_004255"
     CDS             complement(220..384)
                     /locus_tag="ACHU2L_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358839.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="ACHU2L_004256"
     CDS             1286..1570
                     /locus_tag="ACHU2L_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1358840.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="ACHU2L_004257"
     CDS             1558..2043
                     /locus_tag="ACHU2L_004257"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1358841.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAZPEU010000358         2183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPEU010000358 DAZPEU010000000
VERSION     DAZPEU010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.365"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHU2L_004258"
     CDS             <1..203
                     /locus_tag="ACHU2L_004258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358842.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACHU2L_004259"
     CDS             293..694
                     /locus_tag="ACHU2L_004259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358843.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACHU2L_004260"
     CDS             696..1301
                     /locus_tag="ACHU2L_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR1358844.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACHU2L_004261"
     CDS             1288..>2183
                     /locus_tag="ACHU2L_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1358845.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZPEU010000359         2176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPEU010000359 DAZPEU010000000
VERSION     DAZPEU010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.366"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            82..390
                     /locus_tag="ACHU2L_004262"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACHU2L_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..>2176
                     /locus_tag="ACHU2L_004263"
     CDS             362..>2176
                     /locus_tag="ACHU2L_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1358846.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIESSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQR"
BASE COUNT          563 a          476 c          604 g          533 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaagt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgc
//
LOCUS       DAZPEU010000360         2165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPEU010000360 DAZPEU010000000
VERSION     DAZPEU010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.367"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            46..676
                     /locus_tag="ACHU2L_004264"
                     /pseudo
     CDS             46..676
                     /locus_tag="ACHU2L_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
     gene            783..>2165
                     /gene="gadC"
                     /locus_tag="ACHU2L_004265"
     CDS             783..>2165
                     /gene="gadC"
                     /locus_tag="ACHU2L_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR1358847.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIY"
BASE COUNT          570 a          381 c          457 g          757 t
ORIGIN      
        1 ttgtgtttgt aatgtttaat ctgattgtat tgagtggcgt ttattatgaa aaataaatca
       61 ggtaattctt gttatagaga agcagtcgat attcatagct cacttgagct ttcatttata
      121 ttgcataaat atcatttgcg tgtagatgag aagaaatctc gaaatgtatt cagcgccata
      181 cgtgatgagc tcatccttga tggggattca cgacaaaact tagctacttt ctgtcaaaca
      241 tggcttgatg atgaaatccg tgagttgctt aatttagcta ttgataaaaa catgatttat
      301 aaagatgaat atccacaaat ggcggaacct gaaggtcgct gtgtgcgtat gctttccgat
      361 tcgtggagtt tccccgattc gcgacacacc ttaggttgct caaccattgg ttcatctgaa
      421 gctgctatgc tcggccgctt ggcgttaaaa tggcagtggt gtaaaaaacg cgaagtacaa
      481 ggtaaatcaa ccgaacctga tttgcgatcc tgtgcaaata tgctggcata aattctgccg
      541 ttactgtgat gtgaaattgt gtgaaattcc acttgatgat catcacctga taatgaatgc
      601 taaagaggtt ttgaagcatg tcgatgaaaa cactattggt gtcgttacga ctttaggtat
      661 tacttatttt tgtaaagcag attgcgttag tgtttttaag ataagtgtta aagttaatat
      721 tagtttttat atttacatcg taattatatg tttctatttt aaaaatgttc gagagtataa
      781 aaatgtctaa tgctaatatc gcacccaaaa gtaagaaaat gacagtgggg acactcgcta
      841 tcatgaatat aactgctgta gtaagtctac gatttatacc ttctgaagca gaatacggct
      901 taggtgccat tttttactat acctttgctg caattatgtt tttagtacct gttgcctttg
      961 ttgcggctga gctagctaca acatatccac aaaaaggggg ggcatttcga tggataggag
     1021 aagcatttgg ggctcgctgg ggttttgtag gaatgcttat gacctggcta caagtcatcc
     1081 cctactttcc aacagtactg acatttggtg cagtctcggt tgcatttatt gatgttgata
     1141 tgggggtggc agaatcaatt gccagtaata aatggtatat tttatttttc gtactatttg
     1201 tttactgggg agctatttgt gttgcattac gtggcgttgg tattttctcg caggtatcta
     1261 agtggtgcgg tattataggg acaattattc cagctattat ccttgttatt cttggtttct
     1321 cctatctgtg gttcagcggt aaaccagcgg tgatcccatt aggttggggt gatcttttcc
     1381 ctaatatagc taacttccag aatattgttc ttgccgccag catatttctt gcttatgcag
     1441 ggatggaaat gaatgcggtc catgttaacg atttagataa tccaactaaa aaatacccaa
     1501 ttgcaattac catcgcttct ataggtacta ttgcaatatt cctgctcagt actttagggg
     1561 tcgcctttat tattcctaca gataaaatta gtctaactca aagtttgttg gttgcctacg
     1621 atatgctgtt cgaatgggct ggagtgccat ggcttggctc tgtgatggca tttatgctag
     1681 caattggcgt actgggtggg gtggttactt ggattgcagg gcctaataca ggtgtattag
     1741 cgatcgcgaa agctggttat ttacctaagt ttttccaaaa aaccaatcgt catggaatgg
     1801 ggcaccatct aatgtttgta caaggtataa ttgtctcagt tctttctgtt acttttgtga
     1861 taatgccttc agttcaagca gcttttcaga ttttatcaca gttaacagtt atgctctatt
     1921 tagttatgta tatgctcatg ttcgctagtg ctatctatct acgacattct caacctgata
     1981 caaaacgccc atatcgagcc cctgctttat ttttctgggc aacacttgga ttcttagggt
     2041 cttttctggc ttttgtatta agttttattc cgcccgagca aatcccaact ggtagtccaa
     2101 ccagctatgt tctttatttg ttagtattag ttgccctatt tttattaatt ccaatattaa
     2161 tttac
//
LOCUS       DAZPEU010000361         2164 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPEU010000361 DAZPEU010000000
VERSION     DAZPEU010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.368"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACHU2L_004266"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACHU2L_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR1358848.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACHU2L_004267"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACHU2L_004267"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR1358849.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..>2164
                     /locus_tag="ACHU2L_004268"
     CDS             1849..>2164
                     /locus_tag="ACHU2L_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1358850.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYI"
BASE COUNT          587 a          482 c          511 g          584 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atct
//
LOCUS       DAZPEU010000362         2154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPEU010000362 DAZPEU010000000
VERSION     DAZPEU010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.369"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            20..421
                     /gene="spa15"
                     /locus_tag="ACHU2L_004269"
                     /gene_synonym="spaK"
     CDS             20..421
                     /gene="spa15"
                     /locus_tag="ACHU2L_004269"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR1358851.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            425..1717
                     /gene="spaL"
                     /locus_tag="ACHU2L_004270"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             425..1717
                     /gene="spaL"
                     /locus_tag="ACHU2L_004270"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR1358852.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            1798..2148
                     /gene="spa13"
                     /locus_tag="ACHU2L_004271"
                     /gene_synonym="spaM"
     CDS             1798..2148
                     /gene="spa13"
                     /locus_tag="ACHU2L_004271"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR1358853.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
BASE COUNT          687 a          317 c          466 g          684 t
ORIGIN      
        1 tatttagtga ggtttaaata tgagtaacat taatttagtt caattagtta gagatagtct
       61 tttcacgatt ggttgtcctc catcaattat tacagattta gacagccatt cagcgattac
      121 catttctttg gatagtatgc ctgctattaa tatagctctt gtcaatgagc aggttatgtt
      181 atgggctaat tttgatgcac caagtgatgt aaaacttcag tcatctgctt ataacatatt
      241 aaatttaatg ttaatgaact ttagttatag cattaatgag ctggtagagc ttcatcgttc
      301 tgatgagtac ttacagttaa gagttgttat taaagatgac tacgttcacg atgggattgt
      361 ttttgcagag attctgcatg agttttatca gaggatggaa atcttaaatg gggtcttata
      421 atcaatgagc tatacaaaat tgctcactca attatctttt cctaatagaa tctcggggcc
      481 aatcttggaa acaagtctta gcgatgtttc gattggtgag atttgtaaca ttcaggctgg
      541 aattgaaagt aatgaaattg ttgcaagagc tcaggttgta ggatttcatg atgaaaaaac
      601 aatattaagc ttgattggaa attctcgtgg actttcacgg caaacgttga ttaagcccac
      661 tgcccagttt cttcatacgc aagtcggccg tggattattg ggagcagtag tcaatccttt
      721 aggggaggtt actgataagt ttgctgttac agataacagt gaaattcttt atcgacctgt
      781 agataatgct cctccgctat atagtgaaag ggctgcaatt gagaagcctt ttttaacagg
      841 tattaaggtt attgattctt tactcacgtg tggtgaagga cagcgaatgg ggatttttgc
      901 gtcagctggt tgtggcaaaa cttttctcat gaatatgctc attgaacata gtggtgctga
      961 tatatatgtt attgggttaa ttggtgagcg aggtcgagag gttactgaaa cggttgatta
     1021 tttgaaaaac tctgagaaaa aaagcaggtg tgttttagta tatgcaactt cggattactc
     1081 ttcggttgat cgttgtaatg ctgcatatat agccactgct atagccgaat tttttaggac
     1141 tgaaggacat aaagtagcgc tttttattga ttcattaaca aggtatgcca gagcattacg
     1201 tgatgtggcc ttagccgctg gagaatcacc tgccagaaga ggctatccgg tttcggtttt
     1261 tgatagctta cccagacttc ttgaaaggcc aggaaagtta aaggcaggtg gctctattac
     1321 tgcattttac actgttcttt tggaggatga tgattttgct gatccattag ctgaagaggt
     1381 aagatccatt ttagatggac atatatattt gagcagaaat ctagcccaaa aaggacaatt
     1441 tcctgcaatt gattccttaa aaagtataag cagggtattt acacaggttg ttgatgaaaa
     1501 acatcgtatt atggccgctg catttcggga gctactttct gaaatagaag agctaaggac
     1561 aattattgac tttggtgaat acaaaccggg ggagaatgcc tctcaggata aaatctacaa
     1621 caaaatatct gttgttgaga gttttctgaa gcaagattat cgactcggtt ttacttatga
     1681 gcagacaatg gagcttattg gtgaaacaat tagataaggt tttaaaaatc aaagataaat
     1741 atcaacggtc tgttaaatta attgaagcac acatattaac actaaaaaaa aaactctttg
     1801 tatcgagatg tggaggcatt agataaaagg attatatact tcctgcaact ggagaatgac
     1861 ttagaacctg taggcgctca gagtgttagt cagcttttca atacaagacg taagatcgct
     1921 atagtaaaaa agcatattat ccaatatcag tctgaacgaa ttcttctaaa gggaagaatt
     1981 gaagaaattc aaaaagatat tgatgaggca aatgcttcta aaagaaaact gcttcataaa
     2041 gagagtaaaa tatgtaaacg gattggcttg attaaaagaa ataactttgc caaacagttg
     2101 atacttgatg agttatcaca agaggatatg aagtatggca ttagataata taaa
//
LOCUS       DAZPEU010000363         2132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPEU010000363 DAZPEU010000000
VERSION     DAZPEU010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.370"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..132
                     /locus_tag="ACHU2L_004272"
     CDS             <1..132
                     /locus_tag="ACHU2L_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1358854.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="ACHU2L_004273"
                     /pseudo
     CDS             319..556
                     /locus_tag="ACHU2L_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            670..928
                     /locus_tag="ACHU2L_004274"
                     /pseudo
     CDS             670..928
                     /locus_tag="ACHU2L_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            977..1555
                     /locus_tag="ACHU2L_004275"
     CDS             977..1555
                     /locus_tag="ACHU2L_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358855.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1664..1922)
                     /locus_tag="ACHU2L_004276"
                     /pseudo
     CDS             complement(1664..1922)
                     /locus_tag="ACHU2L_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1906..2132
                     /locus_tag="ACHU2L_004277"
                     /pseudo
     CDS             1906..2132
                     /locus_tag="ACHU2L_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          670 a          378 c          522 g          562 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat
      961 ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt ttcatgatat
     1021 atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag cagttttatc
     1081 aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg ccgaaaaaga
     1141 caatgagata acaaagaaag acaaagccaa gataatagat acattaactg aaaactattc
     1201 cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat ctttatatat
     1261 tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa tatgtcatac
     1321 tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg gtagcagcat
     1381 tgacaaatat attacggata tgtgcggcct tatttgtatg aggcatattg agtctactgg
     1441 cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat ttatgaatga
     1501 aaaaggatgg cgggctgcgt tttgttcagc aaagaactta tataaatgtg tttaatttta
     1561 gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt cagcagggcc
     1621 tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg tggtggttga
     1681 ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag aagcatatgg
     1741 ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc tctatccgct
     1801 gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg tataccggct
     1861 caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag agcagcgcgc
     1921 atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt ggcgaaacaa
     1981 ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc tgttgaaagt
     2041 cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg gatgaccgaa
     2101 tccaggcagg aaaagctgat atggctgcgt gc
//
LOCUS       DAZPEU010000364         2129 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPEU010000364 DAZPEU010000000
VERSION     DAZPEU010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.371"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(192..524)
                     /gene="pemK-mt"
                     /locus_tag="ACHU2L_004278"
     CDS             complement(192..524)
                     /gene="pemK-mt"
                     /locus_tag="ACHU2L_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFR1358856.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(526..783)
                     /gene="pemI"
                     /locus_tag="ACHU2L_004279"
     CDS             complement(526..783)
                     /gene="pemI"
                     /locus_tag="ACHU2L_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFR1358857.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(876..1529)
                     /locus_tag="ACHU2L_004280"
     CDS             complement(876..1529)
                     /locus_tag="ACHU2L_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR1358858.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
BASE COUNT          558 a          583 c          510 g          478 t
ORIGIN      
        1 aacttcaaaa ctgcgcaaaa cacgcaaata cgcacaagaa agtagggctg cgcttggaat
       61 tcggaaaata aaccacatca aaactacttt ttagccaaat aacgtttggg aatcaccaga
      121 atggtgggac aacagcggtt ttagtgccct aaatcgtacg ttttcatcca gttgcccctc
      181 aaaccccatg ttcaagtcag aatagtggac aggcggccaa gaacttcgtt catgatagtc
      241 tccggaaccc gttcgagtcg ttttccgccc cgtgctttca tatcaattgt ccggggttga
      301 tcgcaacgta caacacctgt ggtacgtatg ccaacaccat ccaacgacac cgcaaagccg
      361 gcagtgcggg caaaattgcc tccgctggtt acgggcacaa caacaggcag gcgggtcacg
      421 cgattaaagg ccgccggtgt gacaatcagc accggccgcg ttccctgctg ctcatgacct
      481 gcggtaggat caagcgagac aagccagatt tcccctcttt ccatgtcaga tttcctcctg
      541 accagtcgcc ggtgcatcca gccattctcg ttcttcagct gatatttcag cattcggatc
      601 acactgtgcc agtagctcag ccagtgaata ttgcgggcgt ctgtacggct caacaatcag
      661 ccggccatta tcaatgacca tgccaacttc attatctgtg cccagagaca gcgcattcag
      721 cagtgccggt gggacggtca gcataactga gccgccaacc ctcttcagtc gggtggtatg
      781 cattcttcac ctccataaaa gttatattta aatataacat ccactaaaaa aacacaccag
      841 gctttaacgc acaatgttta ataaaaatat aactttcaac caaacagtaa acccagcgtg
      901 gttgcttcca tatgcagcaa taccggcagc agcaggccac cacttctgat cctggccact
      961 gatgtaatca accccaccag gaacagttct gccagtgtca gcaggttctg atactggctg
     1021 tgcgcggcga cgaacaacaa cgacgttatc agcgccccca gccacatcgt ccagcagtac
     1081 cgtgaacgga agacgttcag cataatcccc cggaacagcg tttcctcatt caacggggca
     1141 aggataaaga tggtcagcaa cgtcaggatc acgtcaggta tggacttatc ggcaaaaagt
     1201 ttcgtcataa atggctcagc aggcagagcc agcgccttac cgagcagaaa tacaccgaca
     1261 tacaccacgg ccatcgcacc gaccagccac ggtacgccaa cgttgcgcag ctgaccaacg
     1321 accggtagcg gagctatcca acggcggtat accaggaaaa cacacagcag gtacatcaga
     1381 acagtaccat gactgaagaa caaatagttt tttcctgatc cataaagcag aacggcctgc
     1441 tccatgacaa atctggctcc ccaactaatg ccccatgcag ccagcataac cagcataaac
     1501 tgcagatatt gattacgtgt ttgaatcatt gcatcgcctg taaattttta acttgtccta
     1561 tttttgtcat taccacgtat atacacatgt ataacaattc agatatcgtt accaggatat
     1621 gccgcatcag cggcatggaa ggcggcactc tgttgtttca tatgatacag gagtaaaacc
     1681 gccgaagccc ggcgtaagcc ggtactgatt gatagatttc accttaccca tccccagccc
     1741 tgccagacca tacccgcttt cagccatgag agagcttctg tgcgcggtcg gagtggtccc
     1801 gacgagggtt tacccgaagt cggggcgtat ctccgcgtta gcgggccgtg agggccgctt
     1861 acgagcgtgt actgagaact tccagcgaga agactgacag cgatgaagat gtagttacaa
     1921 cattcataat taaaagcgac tctgttccgg ccctttgggc cggggcgggg ccgcttttca
     1981 gttatgaggg aggggctttg tggtttcggt tctgcgctgg accggggttt ttctggaggt
     2041 tgtttttgtg tgttgtaact aaagtggctc cggtcggggc ccgccgcttg cggtgggagg
     2101 tgcatatctg tctgtccaca ggacaggca
//
LOCUS       DAZPEU010000365         2126 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPEU010000365 DAZPEU010000000
VERSION     DAZPEU010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.372"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(99..987)
                     /locus_tag="ACHU2L_004281"
                     /pseudo
     CDS             complement(99..987)
                     /locus_tag="ACHU2L_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          571 a          498 c          490 g          567 t
ORIGIN      
        1 gatcttttcc tgtgtggtac gccgtctgcg tttctccggc cctaagacat caataatctg
       61 ttctccaatg acaagtctaa aaactggtat taagactatc acttaaataa gtgatactgg
      121 ttgtctggag attcaggggg ccagtctagc agctgctcta cggtcatatt tttaattgcg
      181 ctccggttta caagagccca cccctgtttt tccagataaa actggaaagt atccagggta
      241 cagaccagtg caccgtcagg aacagtttcg gtgaatctga tattgccgtg ttcgtcgaag
      301 tgcacaacca gagtgcgacc gttacccgga atcatcttgt cagcggacgg ggtattattc
      361 tggtgcagtt ctgcctccat gcggtcgaac atcagcgatg taggcctctt tgaatgcagc
      421 ggcttttttc ccagtgaagc ccatcaccag gaaaacgaag ccgtttttgg tgatttggta
      481 cattgggcgt ttttcgcctt tggtgtcggt gtaggtgacg tcctcaaaat tgagggcgtt
      541 aaattcaggt gagcattcag tatttgcgat ggcacgtaac acattgtcgt gtcgtttgtg
      601 gaagaactct gcaaccgcaa cagacgtagt gacagcgcga ccgttttcga tggttacgtc
      661 agggtgagaa agggcaggga tagtagccat gatggcatcc tcgagtgata agttaattaa
      721 ctcaccaccg aggttttcca cgaccataag ggtggtgaga cgtacagggg tggaaatacc
      781 ggtcactcga gaacccggcc agtcttgcga ctgccctgca catcccacca taatttgaat
      841 ggagctgtgc attacgcata aaaaaaccgc ttcagcgcgg ctatgcgctc gagtaacgtt
      901 cgggtttcca cgcccggccc ccgttttatg aggtgcagat gcactataat tccacccgtt
      961 ctggttttca atagctacat tcaacatttt ctcttacctt tcatcaccga agtgaacttt
     1021 gttgatgcgg tgcctggtgc ctccaggtga cgttaaccag ttaacaatta acgccggata
     1081 cagggaaacc cataaccacc cgatacgtgg gaactaatcg ctttttaact gttccgcgtg
     1141 cgctcagccg cattcaccgc atcacaaaat tcactttaaa aagggcggac atcagccagc
     1201 aatgaaactg atgccgccaa aggtaccaat caacatggag tgttgtggcg tggttgtcac
     1261 ttaagcgtat ggtcaacctg acaacccggt gtcctcaacg gggaaggaat aactccgcca
     1321 tacttaccgc cgcgtcattt cgcgttgtgt gcctgctttt aactacgtca ggcgaggtgg
     1381 ttcctgttat tccccaacaa caagaaattt gtataatctg aataccccaa caataagaag
     1441 agtgagcaag tgatgtatta gcgatgtcag ctctcaaaga gactgcatca atgttaaaat
     1501 tattcaatga agcaaaaact caggctgagg ttgatcgcgc tgtgtctgaa ataaactcta
     1561 agttgtcaag cattcagctt gaatatttgt catctcattg atgagctgac aatctctaaa
     1621 agcaatgagg ctgcgctcaa agcaaaactc acggagatag aaaagtttaa gttccaaagt
     1681 gaagactgcg aacctcgcta attgattact ggaacgattg tgtatgccag acagtgcaat
     1741 actagctctg gcaagattac catgtatttt tgtccagaat gttttgcgaa gagagcaata
     1801 tctatattgc aacctttccc tatcgaggaa ggcgataaat atacgaaaac tcaatgcccc
     1861 tcctgtaaaa atgagtatct catggactac aatttcaata atgatttttg agtcacggta
     1921 ttcagatttt taaagaacat gccggatact cacccgtgtc cggcgcacgc actccacctc
     1981 acccgtggag aactccttaa ttaccaacct tggcttcgtt ggttagccat taacgctggt
     2041 atgtaatcat tctggcaatg cttaatttga agtggcacac tgaatttggc cacctgaaca
     2101 gaggtgatat gctcacctca gaacaa
//
LOCUS       DAZPEU010000366         2105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPEU010000366 DAZPEU010000000
VERSION     DAZPEU010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.373"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /locus_tag="ACHU2L_004282"
     CDS             complement(<1..118)
                     /locus_tag="ACHU2L_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR1358859.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDE"
     gene            complement(69..380)
                     /locus_tag="ACHU2L_004283"
                     /pseudo
     CDS             complement(69..380)
                     /locus_tag="ACHU2L_004283"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            complement(377..910)
                     /locus_tag="ACHU2L_004284"
     CDS             complement(377..910)
                     /locus_tag="ACHU2L_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1358860.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(976..1605)
                     /locus_tag="ACHU2L_004285"
     CDS             complement(976..1605)
                     /locus_tag="ACHU2L_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358861.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1609..1824)
                     /locus_tag="ACHU2L_004286"
     CDS             complement(1609..1824)
                     /locus_tag="ACHU2L_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1358862.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          537 a          559 c          463 g          546 t
ORIGIN      
        1 tttcgtccag caactggaga gagttcgacg gtggcatcat tgcccatgcc ggaggcgctg
       61 gaggtttcgg ctgaggatgg cacagggcat tttcctttga cgagcacccg accaccatta
      121 tcaagcttgc gccgaagagc atcattttca gctttcgcat cagctaactc cttcgtgtat
      181 ttagcatcga gtgcatcagc atcacgctgg cgctgctgca tgtcagtaat ggttgcgttc
      241 gccagcttca gttctccggc gtttttgtcg cgctgctctt tgtaggtaat ggcgttatca
      301 cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag agcggagata
      361 atcgcggtga ctctgctcat tgatctatcc cccaacaggc taatgcgctt tcctggtcac
      421 gacgaataac ctgtccatag cagttatttg aacgtatgcg gcaatcgcgc ccaacatctt
      481 ttatccacca gcgaatcgcc tcgcatgcgc ccttacgatc accggcattc agccgcttat
      541 aaaacgtcga cgggaaacac ttaccggggc caatgttata ggggcaaaat gacgctatac
      601 ccgctttctg gggttcggtc agaggtactt taatattgcg ctccacccat gccagcgcct
      661 tatcacgttc aatagcgtta acctggtcac atttttcctt cgacagcttc attcccggta
      721 tgacaggctt accatccacc attgtggcac cacgacagat ggtccatatg ccagaaccat
      781 cacggtatgc cgttgtgtga ttaccctctt tttcgttcag aaactggtca agtatctggg
      841 gagcagacgc gcctgcggca atcagcgcca gaacagcagc cgacaggccg tatttgattt
      901 ttgcgctcat ggatatttat caggatttat cggcaacaaa taacgagcca acttatatac
      961 gccttttaag ataagtcagc cccggctgga tccagtcatc aggctctttc ttaaaggggg
     1021 agtattgaaa tcacgaagaa gagcctcccg caccgcagca tccatgtctg caccactggc
     1081 aagtgcttca atctctgccg ccacctgcaa ataccccatg caacggccaa tgcgtttcat
     1141 aactctggtg ttttcgtctt tcaggcatct gatagcgaga ttaataattt tcaggcagtg
     1201 atgatgcgat gcccggacag gttttccgtt ctcataaacg acgaactcac cctcttcaca
     1261 ggaaaccctg aaaggccgat ggggttcctg gtctttatcc ggtgcagacg caataaaatc
     1321 acttttatgc tcttcctctc cggcactgtt aaccgtaatg acgtactcag gccgcaggta
     1381 agccgcctct ttttctgaca atgcggccgg taaaaacttt tcctgcaaaa acttcataaa
     1441 agtctcgtga gcgactttgt ggcagtaatc gtaaatcgcc gcataatgct catcgcggcg
     1501 tttgttttca tcttcagaac tcaccaacgc cgacaatttt ttattaagct cagtgatttc
     1561 attttccagg tgattgaatc gctgattcat ttcttcatgc ttcattattt actctccccg
     1621 tgcagcctta cgcttgtctt ctcttatttt gaaatacaga ttcgtcagat aagtcagaag
     1681 ccccagaagc agacttccca gcacaccaat cgcagcccac tgtgatggcc tgacctgatc
     1741 caaccactgc aaaaaccagt agccggcact gccggcggag gtgccgtagg caatgcccgt
     1801 tgaaattttg tccatggatt tcatagcctc acctccgcaa ataacggatg gcgtagtttt
     1861 acactgagaa atgaaaggga tttgaaaaga aaaaaacgca aaagcgggcg aaacgatata
     1921 tacagtaagg aaagcactct atccaacaaa ccacccacag ttaatcggaa taaaagcaga
     1981 gtgcttatga atgatcgcac gcccgaaggt tagtatttct gcacagcaat tttgcaaaaa
     2041 aaggcgatca ttcataactt aaacgtcttt cagtcactcc gggatttccc atcatcgcag
     2101 actga
//
LOCUS       DAZPEU010000367         2092 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPEU010000367 DAZPEU010000000
VERSION     DAZPEU010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.374"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACHU2L_004287"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACHU2L_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR1358863.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHU2L_004288"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHU2L_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1358864.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAZPEU010000368         2085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPEU010000368 DAZPEU010000000
VERSION     DAZPEU010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.375"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..797
                     /locus_tag="ACHU2L_004289"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACHU2L_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="ACHU2L_004290"
                     /pseudo
     CDS             912..1253
                     /locus_tag="ACHU2L_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="ACHU2L_004291"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="ACHU2L_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          435 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagc
//
LOCUS       DAZPEU010000369         2080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPEU010000369 DAZPEU010000000
VERSION     DAZPEU010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.376"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>2080
                     /locus_tag="ACHU2L_004292"
     CDS             <1..>2080
                     /locus_tag="ACHU2L_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1358865.1"
                     /translation="YPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKA
                     AQSSITAPDRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVA
                     YAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEII
                     TPKKPGEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYW
                     FHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYP
                     SADIRAKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDN
                     ISKDEWKNLHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSK
                     HEQIERSITPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYER
                     DDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNL
                     FNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLN
                     KGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVII
                     NKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPR
                     RSALLLGKTGE"
BASE COUNT          730 a          408 c          389 g          553 t
ORIGIN      
        1 ttatcctcag gatttaaaag acagagaaat tatatcaact tttcacgcag aaaaagtcat
       61 taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa cttaccgaga
      121 gctgaataaa gctgcacaga gtagcattac agcgccagac aggataggag aagggaaaac
      181 atgggtttat caacgagata caatggttga aagaggtgat aacagtggtg ttcatcagta
      241 tggtccagct gaacatttca ctcacattat atctgacaaa ccgtccccaa aagataagta
      301 tgttgcatat gctattaaca ttcctgacta tgagctagca gccgatgtat ataatattaa
      361 cgtgacgtca ccttccggac agcaagaaac atttaaaata ctaatcaatc cagaacatct
      421 acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat gtgaaatcat
      481 cacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct accagcaaat
      541 cagggaaaat atgtctgagt ttgcacgttc ccattatggg tatatacaaa tccctccagt
      601 gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt actggtttca
      661 cgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggggatt tgcaggattt
      721 tatcgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg attatacctt
      781 taactttatg ggaatcgggg gaagtggtaa aaatgacctg gattatccct ctgctgatat
      841 acgagcgaag atcagtaaag atatagaaag tggtatccct ggctattggc aaggtcaggt
      901 tttgattcca ttcattaaag atccagtaac aaaagaacgt aaacaaatcc atccagaaga
      961 tatacatctc actgcaaaag acttcgaagc aagtaaagat aacatctcta aggatgaatg
     1021 gaaaaacctc catgcattaa aagaaaagag tttaaatgga atgcctaaaa caacccccaa
     1081 aagtgaccag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt
     1141 acgaggttta cgttatgatg cagcaaaaca ctcaaaacat gaacaaatag aaagatcaat
     1201 aaccccaccg cttaaaaatt ataatgagcg attacacaat actaacttat ttaacccaaa
     1261 atatcatgaa aaaaccgtta tgaattacat ggaatatctg gtaacttgtc agttgaatga
     1321 agaacaaatg tcatcgctgc tttatgaaag agatgattta agcgccattg atttttcatt
     1381 gctcatgaag acgataaaag ccttttcatt cgggggagat ctcctaaccc ttgcatcaaa
     1441 accgggttcc acaatctcaa gcatcccatc agaaagacgg atattgatta acattaacca
     1501 cgattttcct aacaatggca atcttttcaa tgactttcta tttaaccatc aacaagatga
     1561 acaattagca atggcatata tggccgctct cccgttcagc aggcctttag tttactggga
     1621 tggccaggta ttaaaatcaa cgactgaaat taaaaattat gatgggccca cgcgtgtcgg
     1681 tggtgaggcg tggcttaata aaggttgctc tacctatcag cagctctaca atgaattcca
     1741 cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat ttgcaactaa
     1801 aaacgttctg gcctttagtc gtggggattc tgtgaacatt aatcactctc ctcatgatgg
     1861 actagttata ataaataaag gaaacgaaga agttgaaggt acctggccta acaaattgca
     1921 acctggaata tataaaaaca tggggagtaa tagcgttaac attattatta ataatacccg
     1981 aaaaattatc cccccttgta aagtatttac gcttagaggc ggatctctaa atatcaatat
     2041 tcctaggcgt agcgctcttc ttttagggaa aacaggagaa
//
LOCUS       DAZPEU010000370         2071 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPEU010000370 DAZPEU010000000
VERSION     DAZPEU010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.377"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..316)
                     /locus_tag="ACHU2L_004293"
     CDS             complement(<1..316)
                     /locus_tag="ACHU2L_004293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358866.1"
                     /translation="MACDYRYKNRQYHCLSGSYMARSAKPRKRKPAPQRSKLLRYVVK
                     LHEDDFFDEEEAEVLRFDNFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI
                     ARC"
     gene            complement(1012..1410)
                     /locus_tag="ACHU2L_004294"
                     /pseudo
     CDS             complement(1012..1410)
                     /locus_tag="ACHU2L_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1413..1779)
                     /locus_tag="ACHU2L_004295"
                     /pseudo
     CDS             complement(1413..1779)
                     /locus_tag="ACHU2L_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1781..2071)
                     /locus_tag="ACHU2L_004296"
                     /pseudo
     CDS             complement(1781..2071)
                     /locus_tag="ACHU2L_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          472 a          581 c          488 g          530 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc
     1741 gctgcgggcc acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg
     1801 cggcaacggc tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa
     1861 gccccataac cgccatgaga cgtgccacag tgcatctggc cactctgata ccttcccgta
     1921 acaactgatg ccagacttta cgcacagcgt atacctgatg attctcatcg tatacgcgca
     1981 gtatctcttt cttcagccag tcatcgcgct gcgcacgggc actgcgttta tcatgatgat
     2041 gtcgctgttg ctgacagtgg taatacgttg a
//
LOCUS       DAZPEU010000371         2049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPEU010000371 DAZPEU010000000
VERSION     DAZPEU010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.378"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACHU2L_004297"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACHU2L_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACHU2L_004298"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACHU2L_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACHU2L_004299"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACHU2L_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR1358867.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..1974
                     /locus_tag="ACHU2L_004300"
                     /pseudo
     CDS             1708..1974
                     /locus_tag="ACHU2L_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          580 a          451 c          482 g          536 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgcctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggcc taatgccgat
     1981 cagttaagga tcagttgacc gatccagtgg ctgtgtaaga atccggaaac gctcacttgt
     2041 ttccggatt
//
LOCUS       DAZPEU010000372         2049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPEU010000372 DAZPEU010000000
VERSION     DAZPEU010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.379"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            65..583
                     /gene="mddA"
                     /locus_tag="ACHU2L_004301"
     CDS             65..583
                     /gene="mddA"
                     /locus_tag="ACHU2L_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR1358868.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            580..1029
                     /locus_tag="ACHU2L_004302"
     CDS             580..1029
                     /locus_tag="ACHU2L_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1358869.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1030..1263)
                     /gene="ydcY"
                     /locus_tag="ACHU2L_004303"
     CDS             complement(1030..1263)
                     /gene="ydcY"
                     /locus_tag="ACHU2L_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR1358870.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="ACHU2L_004304"
     CDS             complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="ACHU2L_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR1358871.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1717..1812
                     /gene="yncL"
                     /locus_tag="ACHU2L_004305"
     CDS             1717..1812
                     /gene="yncL"
                     /locus_tag="ACHU2L_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR1358872.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          492 a          465 c          504 g          588 t
ORIGIN      
        1 gttgcttcgg gctgttttcg atagtattga taacatcatc atcattattt tattgaggcc
       61 agacatgtcc atccgttttg cccgcaaagc cgactgtgct gccattgcgg aaatttataa
      121 ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa acggtggatg cagataaccg
      181 cattgcctgg tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga
      241 tggcgtagtt acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca
      301 taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa
      361 attgttaagt cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg
      421 gatcgaatcg caaaatcagg cttcgctgca tctgcaccag tcgctgggat ttgtcgtcac
      481 cgcacaaatg ccgcaggtag gcactaaatt tggtcattgg ctggatctga catttatgca
      541 gttgcaactc gacgagcgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt
      601 gcttttttga ttgccgccgg aattggtctg gtagtccaga acacgctgat ggcgcgcatc
      661 acccagacct cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg
      721 ctctttgttt ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc
      781 agcgtgcgct ggtggacact tatacctggc ttgctgggtt cgttttttgt cttcgccagt
      841 atcagcggat atcagaatgt gggagcggcg acaactattg cggtactggt cgccagccag
      901 ctgattggcg gtctggtgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg
      961 ttcggcccaa tatgtggcgc aattttactt gtggttggcg cctggttggt ggccagacgc
     1021 tcattttaat cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc
     1081 ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta
     1141 gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg
     1201 ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga
     1261 catactttct ccttagccac ttgtgaaatt tatcgattaa tccaaatgat ataagtctgc
     1321 gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga
     1381 aactcatcgg ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat
     1441 cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa
     1501 gcatatgttg atagagaaac atgtacgggc gtccagccat atgaataaaa cgcgcgcaaa
     1561 tatgcgccat tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa
     1621 cagcaacttg cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta
     1681 ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta
     1741 gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc
     1801 tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa tcaactctct
     1861 gacagtacgc aagatcttct catactctcc aagtttgcaa cattatcctt aatatttact
     1921 tctttttgct ggcatctaca ttgaaatgag tagaaaacat ccccctactg tcgtctatac
     1981 aaacagctta ctgattcaaa cggaatcttg ttttcacatt tttctatcac gaatacttta
     2041 tacgcgcgg
//
LOCUS       DAZPEU010000373         2046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPEU010000373 DAZPEU010000000
VERSION     DAZPEU010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.380"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(47..460)
                     /gene="hns"
                     /locus_tag="ACHU2L_004306"
     CDS             complement(47..460)
                     /gene="hns"
                     /locus_tag="ACHU2L_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR1358873.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            1065..1682
                     /gene="tdk"
                     /locus_tag="ACHU2L_004307"
     CDS             1065..1682
                     /gene="tdk"
                     /locus_tag="ACHU2L_004307"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR1358874.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(1968..>2046)
                     /locus_tag="ACHU2L_004308"
     CDS             complement(1968..>2046)
                     /locus_tag="ACHU2L_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358875.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT          543 a          422 c          465 g          616 t
ORIGIN      
        1 aatcccgccg ctggcgggat tttaagcaag tgcaatctac aaaagattat tgcttgatca
       61 ggaaatcgtc gagggattta ccttgctcat ccattgcttt tttgattaca gctggagtac
      121 ggccttggcc agtccaggtt ttagtttcgc cgttttcgtc aacgtagcta tattttgccg
      181 gacgctgagc acgtttagct ttggtgccag atttaacggc agcaaggcta ttcagcagtt
      241 cgttcgggtc aataccgtca gcgatcagca tttcgcgata ttgctgcagc ttacgagtgc
      301 gctcttcaac ttcagcagca gccgcgcttt cttcttcgcg acgttcgtta acaacaactt
      361 ctaatttttc cagcatttct tccagcgttt caagtgtaca ttctcttgcc tgcgcacgaa
      421 gagtacggat gttgttcaga attttaagtg cttcgctcat tgtagtaatc tcaaacttat
      481 attggggtgg tttgttgagg taataataga gccttaaatt cagttgtgca atagccagga
      541 atgtaaggaa ttcaaaattg ttctttattt tgtgccgcca ataaatatct tttcataaaa
      601 ttagccagaa aagacgcggc atatagccct atttacaccg atgatttcgc agcacgtgag
      661 gttaaaactt cctgattcat gtcacatttt atggggagat tatcgtaggc tgacgacctt
      721 tcagtcttct gtattagttg tgtttacgag aattccctat taagcgaatg atgaaaagta
      781 gaacagtcgc aataagagca tggacttagt attgcactat ctcctggagg tcaacagagg
      841 gctattactt gcgcaacagg ttaaagattg tgaatagtta ccagcagtca tttacccgct
      901 tataacaagc gaggcagttg taatgatagc tcagaaggat tatgcaaggc ttcgtaaggg
      961 agaacgcata tacccacttc tgtgcatact gttgagctga aaaactgacg aattatgata
     1021 aactccagcc aactttattt catatcattg agggcctgtg gctgatggca cagctatatt
     1081 tctactattc cgcaatgaat gcgggtaagt ctacagcatt gttgcaatct tcatacaatt
     1141 accaggaacg cggcatgcgc actgtcgtat atacggcaga aattgatgat cgctttggtg
     1201 ccgggaaagt cagttcgcgt ataggtttgt catcgcctgc aaaattattt aaccaaaatt
     1261 catcattatt tgatgagatt cgtgcggaac atgaacagca ggcaattcat tgcgtactgg
     1321 ttgatgaatg ccagttttta accagacaac aagtatatga attatcggag gttgtcgatc
     1381 aactcgatat acccgtactt tgttatggtt tacgtaccga ttttcgaggt gaattattta
     1441 ttggcagcca atacttactg gcatggtccg acaaactggt tgaattaaaa accatctgtt
     1501 tttgtggccg taaagcaagc atggtgctgc gtcttgatca agcaggcaga ccttataacg
     1561 aaggtgagca ggtggtaatt ggtggtaatg aacgatacgt ttctgtatgc cgtaaacact
     1621 ataaagaggc gttacaagtc ggctcattaa cggctattca ggaaaggcat cgccacgatt
     1681 aataagaatt tctttactga cagggtgagc agggcacttt tatcctgtca gttcgtttta
     1741 cgcacttctt ccgggctata tacccttctc agcagttttt taacgccgct atacgcctca
     1801 cagggctctt aagcaccgac gttgacttgt gacctgtaaa gtacaatatc cctgtgttta
     1861 ggcgttatac atcgtcgcaa atatgatgaa ggctaatgct gtcggtttat ggaaaagttg
     1921 ctttgggtaa acaaaaaaac ggccccagaa ggggtaatgc cagtcagtta agcaactgac
     1981 tggctctttt tcggagctgt ggggtatttc cagggcctct cctttaccac tctcgggaag
     2041 gccctt
//
LOCUS       DAZPEU010000374         2043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPEU010000374 DAZPEU010000000
VERSION     DAZPEU010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.381"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHU2L_004309"
     CDS             complement(<1..513)
                     /locus_tag="ACHU2L_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358876.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKL"
     gene            614..901
                     /locus_tag="ACHU2L_004310"
     CDS             614..901
                     /locus_tag="ACHU2L_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1358877.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            876..>2043
                     /locus_tag="ACHU2L_004311"
     CDS             876..>2043
                     /locus_tag="ACHU2L_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFR1358878.1"
                     /translation="MTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLTV
                     MAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAYI
                     DDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQVL
                     IVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERASR
                     PGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPHT
                     ATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHKE
                     NMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLI"
BASE COUNT          647 a          460 c          423 g          513 t
ORIGIN      
        1 cagctttcca ttttcatgaa aatcttcctt gttgaatccc agttcagcca gatattcccg
       61 aagataacgt ttaacagcag cttcatcgct ttctgtatca gcgtgatcaa gacaaggaca
      121 atacgatttc tgtaaaaaat tataaatatg ataataaaaa gcagagtcat tctctggtct
      181 gtatgtatta tacatatcta caacaagaaa aataacccca aaagtgcgca gacaatagct
      241 gcgaaggaat tcaggtttta gtgtggaatg cactggataa gaattcatca tgtaataaaa
      301 atgttcatat cgggcatata tgatccgccg ttgctcctga ctgaacaccc cttcagcatc
      361 aataatacga ctgatctcat tataacgttg atcccagaat ttttgatgca tttcaaaatt
      421 ggtactcttc cgcttgtgtg ccggattctc gccttcctta aacaacccca aaacagtaca
      481 aatatcatac aacatatttg taaatgtgcg catatgctgt gctctccggt tggtttgcat
      541 tttatcgcag aggacattct gagcttaacg tatcaaactg acacaacgac atgcaatgca
      601 tatggagaac aatatgtcaa atcaaaccgg acagctttac acgccttcac gcatcctgcg
      661 tatggctgaa cttacccgac tgctgggtat atcaagatct actatttacg tgaaaataaa
      721 tccggcctcg aaataccacg accctacctt tcccaaacca ataaggcttg gtagtgcatc
      781 cgtaggctgg cgggcatctg ccatcgatga gtggctgatg ttacatacgg caccagcctg
      841 gtctgaacct gaaaacaaac taaaggaact taatgatgac gacaaaacta tcactccctg
      901 aacttgacac aactaaactg cctgacaggt gccaggcatt acttgatgaa atgcatgaag
      961 aaacaggaat acgccgtgaa atacttctgt ctgttatgct gacggtcatg gctgcctcgg
     1021 tccaggatac gcatgaagtt gaactttccg gagggcaacg taccagcctt cagatattta
     1081 tgtgtctttc atcagcatca ggcagtggaa aaacctctgc ctgcgcaaaa ttaatcgctc
     1141 ctgttcatga aacagaagaa gaactacatc aggcatatat cgacgataaa aaaaactatg
     1201 accgaatgat ggaaatgtgg acaacagata aaaaaatcct ggagcggaga tataaaaagg
     1261 aaattgaaag atccccggaa aatgctgctg ctgcacgagc agcacttgaa aagtgtatag
     1321 caaataaacc cgaacctccc gtacaacagg ttctcatcgt gaatgacgcg acaccagaag
     1381 ggatagcttt gaaactcagc cagtcaccct ctcttctgtt gctgtctgat gaaggaggta
     1441 ctattctgga taagcgtttc gaacgcaaat cggcactgta caacacatta tggagtgggc
     1501 aaccagtcaa cgttgaacgg gcatccagac ccgggttccg gctgaatgat gtccgtctca
     1561 ctatacttat actgactcaa ccggtaatat ttaataagtt ttttactctc actggcgatc
     1621 agatccgggg caatggcttt ctggcccggt tgctattttg cgaacccgga gcaattaaaa
     1681 taatgacgac agaaccgcat actgccactc ctgttgtgac gcaacaatgt gcaagctgtt
     1741 ttcgtaagaa aagttttggc agtcagatca gagactctct gagagcatcc agagaaagac
     1801 gtgcaaaaag taaagagcgc atctgcatga cattatcaaa tgcagcatcc cgcgcactgg
     1861 agcgctttca caaagaaaat atggatactg tcagacagaa tcctcatatg ggtacatttg
     1921 aagacattat tgtcagaaaa agagagcagg ttgtcaggat agcagcactt ctggaactgg
     1981 agaaagatcc agactcgaca gtaataacgc tggaaagtac taatagcgcc atttatctca
     2041 ttg
//
LOCUS       DAZPEU010000375         2029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPEU010000375 DAZPEU010000000
VERSION     DAZPEU010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.382"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1824)
                     /locus_tag="ACHU2L_004312"
     CDS             complement(<1..1824)
                     /locus_tag="ACHU2L_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR1358879.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACHU2L_004313"
     CDS             complement(1932..>2029)
                     /locus_tag="ACHU2L_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1358880.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAZPEU010000376         2026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPEU010000376 DAZPEU010000000
VERSION     DAZPEU010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.383"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..158
                     /locus_tag="ACHU2L_004314"
     CDS             <1..158
                     /locus_tag="ACHU2L_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1358881.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
     gene            complement(175..534)
                     /locus_tag="ACHU2L_004315"
                     /pseudo
     CDS             complement(175..534)
                     /locus_tag="ACHU2L_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(534..1454)
                     /locus_tag="ACHU2L_004316"
     CDS             complement(534..1454)
                     /locus_tag="ACHU2L_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1358882.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1592..>2026
                     /gene="pgrR"
                     /locus_tag="ACHU2L_004317"
     CDS             1592..>2026
                     /gene="pgrR"
                     /locus_tag="ACHU2L_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1358883.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          504 a          507 c          473 g          542 t
ORIGIN      
        1 ccgcgctcag ggatccgctc tccagggctt actacacccg caaaatgagt cagggaaaac
       61 gacacaatca ggcgcttatc gccctggcga gacgacgctg cgacgttctg ttcgccatga
      121 tgcgcgacgg gactttttat accccgcagg ggtcataaca tgcttgacaa cttaataggg
      181 gcacccccca cacacaccca gcacgccaat gcgttcagca tcaacatatg gcagagttac
      241 tgcataatcg ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccaacg
      301 aggctaccca ccagattctc cctggaaact ggcgtcataa gcgagaacga catagccttt
      361 ttcagccaat gccttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg
      421 atggacgctg ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat
      481 attggcagca atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga
      541 gtttcctcaa agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca
      601 taaagatcca tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc
      661 cgatcgtaca ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc
      721 atcggctgag tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta
      781 aggctgcgca aagtagcgta acccggtgct gttggatact gcgcacgagg tgtgtggtaa
      841 tactcccagg cctgacgcag ttcttcgttc ggcgcatcgg cctccttcat tggagccagt
      901 ggcataatgg cgtattcttc gctgctaata tcgctggttc gggcgtttga gccagcttca
      961 atgtgtggca gagcatcaat agatttcaca ttgttttccc aaccattgcg gaacatcgaa
     1021 ccaatattga ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca
     1081 ttggctgtat atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca
     1141 taagaaagcg ttgtcaggta atcaatcacg gcactgacgt cttcagtacg aatgtatggg
     1201 ttttctaact gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata
     1261 gtgacaaatc ccttttccgc cagcttttcg gcataggttc cggccgtttg ctctttaacg
     1321 cccccacctg ggtgagatac cacaattgcc ggatactgac gggtttcatc aaattttgga
     1381 gggaaataga tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact
     1441 ttattattca tcattcagtt cctcatggag ttgtcggttc gttttgacgg ttggtgatat
     1501 cactatagat attgatcatt aagttgatta gaccctaaat catgattaga cctatcattt
     1561 aaatgattaa tagattgtat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat
     1621 ggcgtttgtc gtcgttgcag aggagcgtag cttcactcgc gcagcagccc gcctgagcat
     1681 ggtgcagtca gctttaagcc agatagtgcg ccgtatagaa gaacgattgg gattgcggct
     1741 tctgacgcga accacgcgca gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt
     1801 tcttggccct atattgcatg acatagattc tgccctgaca tccctgagcg atctgcagaa
     1861 ccgcccatcc gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt
     1921 accagccatg cgcacattcc tgaaatcgca tcctgaaatt gatattcagc ttaccattga
     1981 ttatggtttg accgatgtcg tttctgaacg ttttgatgca ggcgtc
//
LOCUS       DAZPEU010000377         2009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPEU010000377 DAZPEU010000000
VERSION     DAZPEU010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.384"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHU2L_004318"
     CDS             complement(<1..494)
                     /locus_tag="ACHU2L_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1358884.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHU2L_004319"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHU2L_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR1358885.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="ACHU2L_004320"
     CDS             complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="ACHU2L_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR1358886.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          585 a          424 c          427 g          573 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa aataaggagt
     1261 ataaagaaat gattgctttg gcttcttaca acacatcatt cccttttcat ttaataaagc
     1321 ggcatgatac aaagtgaacg tggtttcgat ttacattgcc accataccgc ttcagacaac
     1381 cggtacatta ctttgttata agacgtgata tataattcaa aacaacgtgg ttttgattat
     1441 tcgtatctca taataaaagt cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg
     1501 gtgcctttat aacgtgcaga aaaatgaaga acgttggtcc cggggatcaa gttctggttg
     1561 gttgagaagc tattcccatc aggtttgaga attgcggatg tattatcaag tatttcaata
     1621 ccgacacccg ttgctgttgt tgtatttgta ctggatacaa tggccaataa attatcattg
     1681 attgcatcag cctgaccaga aaatgcaacg gcagcattag cggcaacaac aggatcgcag
     1741 tcgttcaact gaatggtgaa tggaattaat gcagaggtat taccaacagc attgaaaata
     1801 tcggtacgat attgaccgag tgtgacaacc tggtctgccg agtcagtatt cactgaacag
     1861 gcagcattca ccagttcgcc ttcaaagtga attgtaccgc catctacaac ggcagcataa
     1921 ctacccgtag cggcgaatag tgcggcagcc agcgcagacg aaataaatct taatttcata
     1981 tatattcctt caatctcatt tatcgactc
//
LOCUS       DAZPEU010000378         1946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPEU010000378 DAZPEU010000000
VERSION     DAZPEU010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.385"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHU2L_004321"
     CDS             <1..203
                     /locus_tag="ACHU2L_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358887.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACHU2L_004322"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACHU2L_004322"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR1358888.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          486 a          483 c          449 g          528 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaaca
//
LOCUS       DAZPEU010000379         1932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPEU010000379 DAZPEU010000000
VERSION     DAZPEU010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.386"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..362)
                     /locus_tag="ACHU2L_004323"
     CDS             complement(<1..362)
                     /locus_tag="ACHU2L_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1358889.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHU2L_004324"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHU2L_004324"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR1358890.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          594 a          483 c          387 g          468 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aataccctca aaaaccacct gaataattaa
     1801 ccattattgt taaaacaaca cgttgaataa tcacataaca aactccatta atcattgaaa
     1861 atgagcacag aaatagaaat taattatatt caattaatta aaatgtgtat tgcttgatgt
     1921 atttaaaaat at
//
LOCUS       DAZPEU010000380         1929 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPEU010000380 DAZPEU010000000
VERSION     DAZPEU010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.387"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            274..549
                     /locus_tag="ACHU2L_004325"
     CDS             274..549
                     /locus_tag="ACHU2L_004325"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR1358891.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFSIQE"
     gene            747..>1929
                     /locus_tag="ACHU2L_004326"
     CDS             747..>1929
                     /locus_tag="ACHU2L_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1358892.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          508 a          380 c          439 g          602 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcat
//
LOCUS       DAZPEU010000381         1912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPEU010000381 DAZPEU010000000
VERSION     DAZPEU010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.388"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            299..993
                     /locus_tag="ACHU2L_004327"
     CDS             join(299..545,545..993)
                     /locus_tag="ACHU2L_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1358893.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(1112..1510)
                     /locus_tag="ACHU2L_004328"
     CDS             complement(1112..1510)
                     /locus_tag="ACHU2L_004328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358894.1"
                     /translation="MNSTINVFGEPSKNLAMLIEEGNLNAPAILNDIKKHVNHLISLH
                     PVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAISIKNHLGQLNLLNSSNIGTLQ
                     INTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(1507..>1912)
                     /locus_tag="ACHU2L_004329"
     CDS             complement(1507..>1912)
                     /locus_tag="ACHU2L_004329"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1358895.1"
                     /translation="AESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPYGNR
                     SADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSIIEPA
                     VEDVIRQQYKNVGIIATEFTIKTGCHKELIKN"
BASE COUNT          556 a          381 c          392 g          583 t
ORIGIN      
        1 aataaaaacc aaaaaagaaa gaatacttaa atcttcgcat gttcttcggg tggtacgcaa
       61 gtttgttagt ctgaccgcac aataaatttt cgcttagaaa ataataagtt acataatgtt
      121 ctgctttttt agataaatat aacccgaaaa aatcattgtt aatcttatgc atttattatt
      181 tgattgtaag ttgtgattat tataatttag ttattgttag tatgttttat aatgctggat
      241 gggtgatgcc tctaattagt tgaatctgat gtataatacg ggcttttgag gttatctcat
      301 gaccagcgtt aatattcatt gtccccgttg tcagtcagct caggtttacc gccatggtca
      361 gaaccctaaa ggccgtgaca gacttcgctg ccgtgactgc caccgtgtgt ttcagttcac
      421 ttatacttat caagcacgta agccgggtat gaaagagctg ataactgaaa tggcctttaa
      481 tggtgccggg gttcgcgata ccgccaggac actgaaaatt ggtagtaaca ccgtcatccg
      541 gactttaaaa aactcgcgcc aaagcgaata acttcttcgc ctgttgccca tgctgatgtg
      601 gcgtttatct gcgagcttga tgagcaatgg agctacgttg gcagtaaagc ccggcaacat
      661 tggctcgggt acgcgtacaa caccaaaaca ggcggtgtac tggcctacac ttttggtccc
      721 cgaaccgatc aaacgtgccg ggagctactg gcactgctta cacccttcaa catcggcatg
      781 ctgaccagcg atgactgggg cagctatggc cgggaggtgc cgaagaataa gcatctgacc
      841 ggcaaaatat tcacccaacg cattgagcgt aataatctga cgctacgcac ccgcattaag
      901 cggttgggtc gtaaaacaat ctgcttctca cgctcagttg agatccacga aaaagtgatc
      961 ggagcgttta tcgaaaaaca catgttctac taattggatg cactacccag tatgtaactg
     1021 ggtttgtcca taaaatgcac cttactcagt tggtgtccaa cttttggggt gcagttcaga
     1081 aggacgggat tttacggtgt accggataaa tttaaaactg cctgatactg aatgtatgag
     1141 gtttcgtaat gctcaattcc cctagtaccg ttttgtagat ctccatactt ccactagtat
     1201 ttatttgcaa agttcctata tttgaggagt ttaataaatt tagctgtccc aagtgatttt
     1261 taatagagat agcctgatca tgtgcaggat taataaattt tatatccggt gcaactgctt
     1321 caaaaaaatt ctgaacaatt ggatagtgag tacagccgag aataatttca tttactggat
     1381 ggagggaaat taaatggtta acatgttttt tgatgtcatt caagattgca ggagcattaa
     1441 gattaccttc ctcaattaac attgcaagat ttttgcttgg ttctccaaat acatttattg
     1501 ttgaattcaa ttttttatta gctctttgtg acaccccgtt ttaatagtaa actcggtagc
     1561 gataatacca acatttttat attgctggcg aatcacatct tctactgcag gttcaatgat
     1621 ggataagata ggaaacggaa agctcttagc atatccgctg tattcttcgg attccaaagt
     1681 tgaggatata gtattacatg cgacagcaac tagtttgaca ttccttgact gtaaaaaagt
     1741 cagcattttt ttagtcaaac tgatgatatc atcggcagaa cggttgccat aaggtgcatt
     1801 catattatct ccacaataga taatatcttc accaggaagc aaattaatta tttcctttac
     1861 cgatgtcaat ccaccgacac ctgagtctat tacaccaatt ggagattcag ca
//
LOCUS       DAZPEU010000382         1901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPEU010000382 DAZPEU010000000
VERSION     DAZPEU010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.389"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(475..1413)
                     /gene="lrhA"
                     /locus_tag="ACHU2L_004330"
     CDS             complement(475..1413)
                     /gene="lrhA"
                     /locus_tag="ACHU2L_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR1358896.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT          533 a          441 c          402 g          525 t
ORIGIN      
        1 ttgtcaactt taacaaaagc ttcttcacat tagtttacat aatatcaaca ccattagcat
       61 ttaatgccct ttcaccccag atccttgacg actccaggat aattagatgt tgttgaatcg
      121 tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt tgtgtcgatt
      181 cctcccccca aaagagagta ttttcttgat ctgtgacacg cttttgttat tccataacaa
      241 aaacgcagca acaaatttac gtatttttta acatcattgt agcaggtgat ttttttcagg
      301 cgattatttg tgcattcggg acgtgaatct ctggtgattg aaaaataaac agttttgtac
      361 gttctgcact atgcgaggaa ggatttttac taaaaaaaag ccgctggggt ttaaaacacc
      421 cccagcggct cgttttttac actattgtct caggaattat ctatcgtccg tcgattactc
      481 gatatccctt tcaatcaaca atgaatcatc cccttccgga gcagacattg ggctgtattg
      541 ccacggatta tggtagctct ccatcgcctg atagatcacc tgggccagct cattattact
      601 ggacgggtca tagcacagca aatattcggt atcaggtagc ggcggtaagc catctactcc
      661 actcagcaca cgcaggtccg ggctcatcat ctcaaccggc cttgccgtca cgccaagacc
      721 ggctttcact gccgcacgaa cggccggaag cgtcgaggcg acataagcca gtcgccatgg
      781 aatatctact ttattaagcg tcgccagcac catatcgcga aacggacttg gatcatccag
      841 caatacaaga gggatcggct cgcctttttg caatacgtat tccgctgcgc agtaccagtg
      901 tgttggcgag gtacgcaggt tcaatgcctt aaacgctgaa ggacgatggg tggttaccat
      961 caaatccact tcctgggatt ccagcatttc tgccatatag gcattacgtt taacgcggac
     1021 atccagcgct aatttcggat aaaccgaact cacgcgattt aacaggaaag gtaagatcgt
     1081 atcggcagat tcatctgaag caccgatagt taacacgccc tgaagattac tgaacattaa
     1141 tgatgagcag gcctcatcat taaaacgcag gattttcctg gcgtaaccaa gaagttgaat
     1201 gccatgttca gttaacagtt tgttgcgacc gtgacgagcg aacagttctt tcccaacgag
     1261 ttgttccaga cgctgcattt gctgacttac ggcggactga gtacgacaca cagcggcagc
     1321 tgcggcagca aaagtgttca gatcggcaac agcaacaaat gttctcagca gatcgaggtc
     1381 gaggttaatt atcggacgat ttgcacttat catatattat cacttactgg cggctcatac
     1441 tgagctggtt aatgctgtgc acacacaaac aagcaattcc atttgtaatg tgcctccctg
     1501 gcagtttcat ccggaacccg gacgaaagta aaaatgcata tgagttgcac taaaaaagcg
     1561 actcacattg ttccgttata atgcctgaag tagatcacag aatatatctt cagggatcgc
     1621 atatctatta agttactcac tcttttctat ttatgacatg cgcgtgtttg tataaatgta
     1681 aatgtgagtc cttgttccac tctcgtgcag catcgctggt catacgcgaa cacgtacaaa
     1741 cagcaatggt gaagcggcat caagagcagg gatccgtcat ttatgcgagc attttacccc
     1801 aaaaaccttt tatttataag ggtcattgcg aattatctga tgcaaagtta tgttatgtta
     1861 ggcaaagtaa tcttctatta ttaataagca catcaaaacc t
//
LOCUS       DAZPEU010000383         1895 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPEU010000383 DAZPEU010000000
VERSION     DAZPEU010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.390"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(79..1491)
                     /gene="pykF"
                     /locus_tag="ACHU2L_004331"
     CDS             complement(79..1491)
                     /gene="pykF"
                     /locus_tag="ACHU2L_004331"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR1358897.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1499..1693)
                     /locus_tag="ACHU2L_004332"
     CDS             complement(1499..1693)
                     /locus_tag="ACHU2L_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358898.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          473 a          442 c          467 g          513 t
ORIGIN      
        1 ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg atatacaaat
       61 taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag tgccgctcgg
      121 taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac cgctctgcag
      181 agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt taaccagctg
      241 cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg tggtcagtgc
      301 caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac cgccctgggt
      361 agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac cacggcatac
      421 cgcttcggtg atgcgcagtt tacggttgtc attgttgaac tcgagacggc tgttcatcac
      481 gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg ggtatttacc
      541 ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg cgtttgcaac
      601 gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca gcatctgggt
      661 cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct tctgggcgaa
      721 gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca tgatgccgtc
      781 agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga ttttggagat
      841 gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga taacgtcaga
      901 acgcttacga ataaaggaag cagcaacaaa gtctacgcct ttttcgcaac caaagatcag
      961 gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag gcaggttcac
     1021 acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt tgttaccttc
     1081 aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt tgccaacaga
     1141 caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc cgataacaga
     1201 tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacatcgt taccgccttc
     1261 cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg cggttttacc
     1321 agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg catagtcacc
     1381 atgagagaag ttcagacgca taacgttcat gccagcgtcc agcattttag ctaacatctc
     1441 ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca tgacagtctt
     1501 agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc cggaaagcga
     1561 atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc gaaaaagcgt
     1621 gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg tctgtttttg agtcgttgag
     1681 ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt atacgctgaa
     1741 attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg ttagacaata
     1801 aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt cgctgtttgc
     1861 gcaacgtctg gtgccgaagt tgtcagtgtg aggat
//
LOCUS       DAZPEU010000384         1894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPEU010000384 DAZPEU010000000
VERSION     DAZPEU010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.391"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..1894
                     /gene="sat"
                     /locus_tag="ACHU2L_004333"
                     /pseudo
     CDS             1..1894
                     /gene="sat"
                     /locus_tag="ACHU2L_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          693 a          335 c          373 g          493 t
ORIGIN      
        1 gcgttaatgg tgaccaactg acatggataa caaaatacaa tgataaactg gttagtgagt
       61 taaaagatac ctatagtcat aaaataaatc tgaatggcaa taatgtaacc attaaaaaca
      121 cagatataac attacaccaa aacaatgcag ataccactgg tactcaagaa aaaataacta
      181 aagacaaaga tattgtgttc acaaatgggg gaagtgttct gtttaaggat aatttggatt
      241 ttggttgcgg tggtattatc tttgacgaag gccatgaata taacataaac ggtcagagat
      301 ttacatttaa aggagcagga attgatatcg gaaaagaaag cattgtaaac tggaatgcat
      361 tgtattccag tgatgatgtt ttacacaaaa taggccccgg tactctgaat gttcaaaaaa
      421 aacagggggc aaatataaag ataggtgaag gaaatgttat tcttaatgaa gaaggaacat
      481 ttaacaatat ataccttgca agcggaaatg gtaaggtaat actaaataaa gataattccc
      541 ttggcaatga tcaatatgcg gggatatttt ttactaaacg tggtggcacg ctagatttaa
      601 atggacacaa tcagactttt actagaattg ccgccactga cgatggaaca acaataacta
      661 actcagatac aaagaaagaa gccgttctgg caatcaataa cgaagactcc tacatatatc
      721 atgggaacat aaatggcaat ataaaactaa cgcacaatat taattctcag gataagaaaa
      781 ctaatgcaaa attaattctg gatggtagtg tcaacacaaa aaatgatgtt gaagtcagta
      841 atgccagtct taccatgtaa ggccatgcaa cagagcatgc aatattcaga agcacagcga
      901 gtcattgctc cctggtattt ctttgtggaa cggactgggt caccgttttg aaagaaacag
      961 agagttcata taataaaaag ttcaattctg attacaaaag taataatcag cagacctcat
     1021 ttgatcagcc tgactggaaa accggggtgt ttaaatttga tacattacac ctgaacaatg
     1081 ctgacttttc aatatcacgc aatgccaatg ttgaaggaaa tatatcagca aataaatcag
     1141 ctatcacaat cggcgataaa aatgcttaca ttgataatct tgcagggaaa aatattacta
     1201 ataatggttt tgacttcaaa caaactatca gtactaatct atccatagga gaaactaaat
     1261 ttacaggtgg catcactgca cataacagcc aaatagccat aggtgatcaa gctgtagtta
     1321 cacttaatgg tgcaaccttt ctgaataata ctcctataag tatagataaa ggagcaaaag
     1381 ttatagcaca aaattccatg ttcacaacaa aaggtattga tatctccggt gaactgacta
     1441 tgatgggaat ccctgaacag aatagtaaaa ctgtaacgcc gggtctccac tacgctgctg
     1501 atggattcag gctgagtggt ggaaatgcaa atttcattgc cagaaatatg gcatctgtca
     1561 ccggaaatat ttatgctgat gatgcagcaa ccattactct gggacagcct gaaactgaaa
     1621 caccgactat atcgtctgct tatcaggcat gggcagagac tcttttgtat ggctttgata
     1681 ccgcctatcg aggcgcaata acagccccca aagctacagt tagcatgaat aatgcgatct
     1741 ggcatctaaa tagccagtca tcaattaatc gtctagaaac aaaagacagt atggtgcgtt
     1801 ttactggtga caatgggaag tttacaaccc ttacagtgga caaccttact atagatgaca
     1861 gtgcatttgt gctgcgtgca aatctggccc aagc
//
LOCUS       DAZPEU010000385         1890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPEU010000385 DAZPEU010000000
VERSION     DAZPEU010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.392"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            43..1191
                     /locus_tag="ACHU2L_004334"
     CDS             43..1191
                     /locus_tag="ACHU2L_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1358899.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
     gene            1330..1842
                     /locus_tag="ACHU2L_004335"
     CDS             1330..1842
                     /locus_tag="ACHU2L_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358900.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
BASE COUNT          571 a          355 c          472 g          492 t
ORIGIN      
        1 ataagtgata ctggttgtct ggagattcag ggggccagtc tattgtctat aaccacgaac
       61 cgttatgcat caggttatgc cacactgact gaagcagtaa gtgcacagga tgaacggaac
      121 agaaaaagag ataagaatag tcacgatggc tctacgatat ccttatccca gccccttggt
      181 aatatcggga atttgaactt caataccacg aggtataatt cttcccgggg cacaggaaac
      241 acgcggtcta ccagcctgag ttatagtacg gtatggagag gcataacatt ctccattaac
      301 tgggcaaaaa acgacttgct gacatcgcat aaatggaaag tagatcggaa attaagcgtg
      361 ggtatttcag tgccattatc gctgggtgac gaaaatcaaa tttatgcgtc ttcacagatg
      421 agtcgttcgg gagagcaggg taataactat caggtttcat tatcaggtca gaatagtggt
      481 ggtgtatggt gggatgtagc gacaaatata actaacgctc atcagtcaca accgaagagc
      541 acgatgaaca ttgtgcaagt tggcaaaaat ggatcatatg gtcagtttag tagccattac
      601 agctccagcg aaaacatgaa acagctaggt gcaaatttaa gcggtgggat acttattaca
      661 agggatggac ttacatttgg tcaaaatgtg gatgggacat tagcccttat agaggcaccc
      721 ggagcaacgg gggttaacgt taatggctgg ccgggactga gtactgattt tcgtgggtat
      781 gcaatactgc cagtgcagcc ataccgaaga gacgatgtta ttctggatga aaaaacgata
      841 ggtaagaatt acgacttacc acaaactagt caattggttg taccgacagc tggagccgtt
      901 gtgcctgcga cattggcagt gaagagcggg gataaaggat tagtaacctt aaaacagaaa
      961 gagggtaaac ccatcccctt cggcgctgtg attagttata gcaaagacac tgaaaatatg
     1021 gccggcattg taggtgaaga tggtattgct tatgtttctg gcttgagtgc ggaaggcgag
     1081 tttaatgtga aatggggtta ttctaaagat caatcctgta tcgcaaaata ccaacttcct
     1141 gcgaagaaaa gtgcatcagg gttataccag atagcggcga catgtttata gtttctaaat
     1201 taagaaactc ataatttatt tttgtgaaga tgtttccatt atttggtgga agcattttcg
     1261 cgatagcacg taaatgcaaa gaaagaaact gcattttgca gtgtaaatga aaatcatgga
     1321 gaaaatataa tgaagaaaac attgttagca atcatgctgg cggggacagc ttttgcttct
     1381 caggcgggaa ctttagtaag ccagggtact gaagcgtcag ctaatcttac tttgaccaag
     1441 cctatcgttg ttaataatac cattcaacca gtgaaagggg tatatagcgg tacgttaact
     1501 gcatggacgc ctttggcaac aggtattgtt ggcgcatctg atgggcagag ccacgactat
     1561 gctgtaactt tcccggatga tatttatgcg gagagcagta cttcagcgga tgcagtaatt
     1621 tctggtgaca ataacccgga ccataaactg aaagtttccc tgacaacgct tgagcaggat
     1681 cctccttcag ctgcctccga agagattggc ggtaagcgtt atatgatgct gaaaaatact
     1741 ggaacaggtg gtgcctatcg cgtcgtttcc catatgaaag aacaggttgt tgagccagac
     1801 tcttacacca tccgtaccca ggcttatatc tacgcagaat aatgcagatg attagttaat
     1861 ttcatggcgg gatttgatat cccgcctttt
//
LOCUS       DAZPEU010000386         1854 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPEU010000386 DAZPEU010000000
VERSION     DAZPEU010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.393"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..529)
                     /gene="mxiE"
                     /locus_tag="ACHU2L_004336"
     CDS             complement(<1..529)
                     /gene="mxiE"
                     /locus_tag="ACHU2L_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR1358901.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYA"
     gene            complement(659..1087)
                     /gene="mxiM"
                     /locus_tag="ACHU2L_004337"
     CDS             complement(659..1087)
                     /gene="mxiM"
                     /locus_tag="ACHU2L_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFR1358902.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(1071..1478)
                     /gene="mxiM"
                     /locus_tag="ACHU2L_004338"
     CDS             complement(1071..1478)
                     /gene="mxiM"
                     /locus_tag="ACHU2L_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFR1358903.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(1471..>1854)
                     /gene="mxiN"
                     /locus_tag="ACHU2L_004339"
     CDS             complement(1471..>1854)
                     /gene="mxiN"
                     /locus_tag="ACHU2L_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR1358904.1"
                     /translation="LLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKK
                     LELDLHKYRSDVKIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKK
                     YFKELAHKKLRQIAEDLLKENPVND"
BASE COUNT          607 a          358 c          230 g          659 t
ORIGIN      
        1 atgcataccc attattcatg gcagctgaag tgattgtttg attgtgcaaa aatacatcta
       61 ataaaccatt tactaaccgc caagtgttca gttgttcttt gacttttgcc cctaaagctt
      121 ttctacataa agatctaaaa taagcctccg acacaccata atgctctgtc agactcttta
      181 ttcttacctc cttttctttc tcaatagttc tcagaagata aagaaccaaa tgatatgact
      241 cagtcattcg tatcatagcc agtgcattac ttacattctg gtcagatgaa aagatgaatc
      301 ttaacattaa cttatcaagc atcgattcgg tattacaact atcccgaaga tcattctcat
      361 gatagaaatg aatacatttt tcagcatggc tatgctctac tagagcacca ctgaaatagt
      421 cccaaaaagc aagaaatttt gcacaactat taatgttaaa gcgataatga tagttcacat
      481 tatcactgaa ttttatattt tgatttcgga caaaaaaaaa cccttccatg ctggagccag
      541 ctcaattatg ttacgttctt tatcttttac aagctcaacg ttaactggtt catgtccact
      601 agagtaaatg taaaacatat tacttgtatt tagaccttta tatttactca caatacaatt
      661 atatccctcg aattaatttt aatttaactt ccccatcctt ctctataagg atggtatcat
      721 ttatcttatt accttttaaa acaaccccgt ctttcagcat agaaaatagt ttatctatag
      781 ctatcttttg tgcatccatg aagcacccat ctacttcctt aacattaaag ttgcccatga
      841 atttacctgc attaaaacta taaaccgcct taccactaat acatttagag taaacattta
      901 tacttttatt ggttgtatta aacgaccata ataaatcatt tggaatagaa aaataatcct
      961 tacttacagg aacaatatgc cattcttttt cagaattaga tgatgacttt aaagcacacc
     1021 cactcagtgt taatagcaat atacttaaaa taaatatttt caacttatta ctaccatgtc
     1081 gaatcatctg cctctctccc tgcttcaaag acactaatta atgaatcgaa ggttgtagtc
     1141 ttaatataga aatcaatatt gttatatatg gacaatgatg cataaaatcc ctcatgcaaa
     1201 tcgacatatt gatttgcatc tatgagttta ttaagaagtg aacttttttg tgcagctgta
     1261 agataaggaa aacgtgaatt gaataattgt tcaatcttat tgtgcagttc atcattatct
     1321 atggaattta attcactctg tgagaaatat tgcattattt catagattat atcctcatca
     1381 aatgattgcg aagatgataa tctctcattt aaattaacaa cttcttcact atttaatctt
     1441 tgctgcagag cattgcttgc atttatttga ttaatcatta acaggattct cttttaacaa
     1501 gtcttctgca atttgtctca gtttcttatg tgctaactct ttgaaatatt ttttatcatt
     1561 ttttgttaat ttttccgcaa gttctatttt aacacctgat attacatcct gaggactaaa
     1621 ctccactact tgattaccac tacagaatat ataattattc ccttcagaat attttaaaat
     1681 aatcttgaca tctgagcgat atttatggag atcaagttct aactttttcc tcagtgctaa
     1741 attacacttg ggcaaaacca cttttaattc tgttactgta ttacgcaacg aactaagcca
     1801 ctgctccaat aaagtgctaa tgatcctctc atcatgtaaa ttttctgaaa gcaa
//
LOCUS       DAZPEU010000387         1846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPEU010000387 DAZPEU010000000
VERSION     DAZPEU010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.394"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACHU2L_004340"
     CDS             complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACHU2L_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFR1358905.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="ACHU2L_004341"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="ACHU2L_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR1358906.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="ACHU2L_004342"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="ACHU2L_004342"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR1358907.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            877..1032
                     /gene="hokD"
                     /locus_tag="ACHU2L_004343"
     CDS             877..1032
                     /gene="hokD"
                     /locus_tag="ACHU2L_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1358908.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1250..1501
                     /gene="rem"
                     /locus_tag="ACHU2L_004344"
     CDS             1250..1501
                     /gene="rem"
                     /locus_tag="ACHU2L_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR1358909.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1568..1846
                     /locus_tag="ACHU2L_004345"
                     /pseudo
     CDS             1568..1846
                     /locus_tag="ACHU2L_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          506 a          409 c          466 g          465 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgtta
      901 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
      961 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
     1021 cctgaggagt aagagacccg gcgagtgaga aatccctcgc cacctctgat gtggcaggca
     1081 tcctcaacgc acccgcactt aacccgcttc ggcggttttt tgtttttatt ttcaacgcat
     1141 ttgaagttct ggatggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa
     1201 aagggatgga ccccgaacag gggagtgcta cttatctgga aggattctga tgatgaacat
     1261 cgaagaactg cgtaaaattt tttgtgaaga tggcctctat gctgtgtgcg ttgaaaatgg
     1321 aaatattgtt agtcattacc gcattgtgtg tttgcaaaaa aatggggctg cgttaattaa
     1381 ttttgtggat gcccgagtga cggacggatt tatcttgcgc gacggtgagt ttgtcacttc
     1441 attacaggca ttgaaagaga tcggaataaa agctggcttt tctgcttttt cagaagaata
     1501 aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt gccaccggag
     1561 aaaaccgatg gcacgcaacg caaaatatta caatcatgat aattcgaccg ttcttgccca
     1621 cacgcacgag cggtattctc acgcatttaa gtcagactgg taccagcatc cctcatgcac
     1681 tgaagaacag gccgaatggc ttattcagtg ttaccgcagg cgcggatgcg aggttaaaaa
     1741 agcccttagc ctcgactacc gtcactagat aatctccgtc aggctccctt actccgaaca
     1801 gccaccgcgt ccgtcccgca cattccagca acggatctgg aggtaa
//
LOCUS       DAZPEU010000388         1838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPEU010000388 DAZPEU010000000
VERSION     DAZPEU010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.395"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(171..932)
                     /gene="kduD"
                     /locus_tag="ACHU2L_004346"
     CDS             complement(171..932)
                     /gene="kduD"
                     /locus_tag="ACHU2L_004346"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR1358910.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(962..1798)
                     /gene="kduI"
                     /locus_tag="ACHU2L_004347"
     CDS             complement(962..1798)
                     /gene="kduI"
                     /locus_tag="ACHU2L_004347"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR1358911.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
BASE COUNT          493 a          480 c          404 g          461 t
ORIGIN      
        1 gaattattga gattattatt aacctcctaa ttttaaagca gataaaattc ataaagttca
       61 ttaattgata attgatatgg attatttcat aaccatgata tggattatga tgatctagag
      121 atataaaaaa ccctgccatg cggcagggtc ataaaagtaa gaggaatgaa ttaacgcgcc
      181 agccaaccgc catccacagc aatggtataa ccatttacat aatctgaagc gctggaggca
      241 agaaacacta ccggccccat caggtcactc ggcagtcccc aacggccagc tggaatgcga
      301 tcgagaattt ccgcgctacg ttgttcatct gcccgcagtt gttgggtatt gttggtcgcc
      361 atgtaacccg gagctatcgc attaacatta atgttgtgtt tagcccattc gtttgccatc
      421 aatcgcgtca cacccatcac gccgcttttt gatgcggtat aagaaggcac acggatcccg
      481 ccctggaagg agagcattga cgcgatattg ataatcttgc cgccattgcc ttgcgcgata
      541 aagtgtttcg ccgctgcctg agacatgaag aatacgctct tgatattcag gttcatgaca
      601 tcgtcccagt ctttttcgct gaactcgaga gcgtcttcgc ggcgaatcaa tccggcgtta
      661 ttcaccagga tatcaatatg accaaattca gctaccgcgc gatccagcag tccaggaatg
      721 ccatcaatct ttcgcagatc ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc
      781 tgctcgatgg tttcagtcgg ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc
      841 gccagcccca acgccatccc ctggcccagc ccagtatcac aaccagtgac gaccgcaact
      901 ttaccttgga gagaaaatgc acttaaaatc ataacaacac cttattcgtt tatgcccaca
      961 actagcgcaa atctttaacg cccacatggt ccatatcatt aaagacctgg ttttcaccga
     1021 ccatgcccca gataaaggta taagctttgg ttccgacgcc ggaatggatc gaccagctcg
     1081 gggagatcac cgcctgctcg ttatgcatca caatgtgacg cgtttcttgc ggctgcccca
     1141 tcatgtggaa aacgcaggcg tcatcatcca tattgaaata gaaataaact tccatccggc
     1201 gctcgtgggt gtgacacggc atggtgttcc acaagttacc tggagccagc tccgtcagcc
     1261 ccatactcaa ttgacaggtt tccagtacat ccgggacaaa atatttgtta atcgtgcgac
     1321 ggttactggt gaggttatcg cctaacgtga ctggagatac ttcgtccggt gtgacttttt
     1381 tggtgggata cgtcgtatgc gcgggtgcgc aattgtaata aaacttcgcc ggagtggcgg
     1441 tatcaatact ggcaaagaca acttcttttg cacctttacc aacatacagg gcgtcgcggt
     1501 gaccgatttc atagcattga ccatcgacag taatcgtacc ggcaccgcca atattgataa
     1561 cacctaactc gcgacgttca aggaaatagc ttacgccgag ttgtttacca acttccccgc
     1621 caacggaaac cgttttagtt atcggcataa tgccgccaac aataattcgg tcaatgtggc
     1681 tgtaaaccat ggtgtactca tcggcgacaa ataccttttc aaccaaaaat tcattgcgca
     1741 gcccttgggt atccagcgtt tttgcgtgcg caccgtggat gctctgtctt acgtccacat
     1801 caacctccga taatcagtga agcgtgaaaa tagaaaac
//
LOCUS       DAZPEU010000389         1832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPEU010000389 DAZPEU010000000
VERSION     DAZPEU010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.396"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..180)
                     /locus_tag="ACHU2L_004348"
     CDS             complement(<1..180)
                     /locus_tag="ACHU2L_004348"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358912.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="ACHU2L_004349"
     CDS             complement(321..875)
                     /locus_tag="ACHU2L_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1358913.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="ACHU2L_004350"
     CDS             complement(872..1162)
                     /locus_tag="ACHU2L_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1358914.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="ACHU2L_004351"
     CDS             complement(1162..1761)
                     /locus_tag="ACHU2L_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1358915.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          421 g          470 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa ta
//
LOCUS       DAZPEU010000390         1830 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPEU010000390 DAZPEU010000000
VERSION     DAZPEU010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.397"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..793
                     /gene="wzx"
                     /locus_tag="ACHU2L_004352"
     CDS             <1..793
                     /gene="wzx"
                     /locus_tag="ACHU2L_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR1358916.1"
                     /translation="SISSSCIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVR
                     NDIKISGVHFCYKTFKRLFFFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAP
                     AEVILKLGIIPAAIGRAVFPRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSG
                     LVLKIWFGENYQINSFNILNVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVP
                     YLALLYFMVEKYGLLGAAISWSIRVILDALLLQWLYTRMCSVYEN"
     gene            823..1712
                     /locus_tag="ACHU2L_004353"
                     /pseudo
     CDS             823..1712
                     /locus_tag="ACHU2L_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
     gene            1705..>1830
                     /locus_tag="ACHU2L_004354"
     CDS             1705..>1830
                     /locus_tag="ACHU2L_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1358917.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFIS"
BASE COUNT          589 a          254 c          355 g          632 t
ORIGIN      
        1 atctatatca agctcttgca tcgcgggaat tccggccata tttgtttttt atagtgctac
       61 attgtcggca gcggttgctg gtttaatatt tgcaagagtt atttcgattt tagtctctgc
      121 ctattatgtc aggaatgata ttaaaatttc gggggttcat ttttgttata aaacctttaa
      181 acgactcttt ttctttggcg gttggatgac agtaagtaat atcataagtc cggtcatggt
      241 gtattttgat cgatttatag tgtcaaatat tatgggggca gataaagttg cattttattc
      301 tgcaccagcg gaggttattc taaaattagg aataatacct gcagcaatcg ggagggcagt
      361 gtttccaagg ttaagtaaca tcaaagactt taaagaattt aaacgtaatg taaataaatc
      421 attgctttta atgtttctaa tctgtttgcc ggtgataatc ataggcttgt tatattcagg
      481 gcttgtattg aaaatatggt ttggtgagaa ttatcaaatt aattccttta atatattaaa
      541 tgtgttattg atcggttttt tcttcaatgc gctggcaatg ataccattct ctgcaatcca
      601 ggcattagga aaatctaaaa ttactgcttt gattcattgt gctgaattgg ttccttattt
      661 agcccttttg tattttatgg tcgaaaaata tgggttactg ggtgccgcaa tatcctggag
      721 catacgtgta attttagatg ctctgttatt acaatggctt tatactagaa tgtgttctgt
      781 atatgaaaac taaaattaac caattactga gtcacttgga taatgaatag taatatttac
      841 gctgtcattg tgacatataa tcccgaactt aaaaatctga atgcactgat cactgaacta
      901 aaggagcaaa attgttacgt tgtagtagtc gataatagga ctaatttcac cttgaaagat
      961 aaattagcag acattgaaaa agttcattta atttgtcttg gtaggaatga aggtatagca
     1021 aaagcacaaa atattgggat tcgatattct cttgaaaaag gggcagagaa gataattttt
     1081 ttgatcaaga tagccgtatt cgcaatgagt ttatcaaaaa actttcctgc tatatggata
     1141 atgaaaatgc aaaaatagct ggcccagttt ttattgatag agataagtca cattattatc
     1201 ctatttgtaa tatcaaaaaa aatggtcttc gagagaaaat tcatgtcact gaaggacaga
     1261 caccgtttaa aagttcagta acaatctcat ccggaaccat ggtttcaaaa gaagtttttg
     1321 agattgttgg aatgatggat gaggaacttt ttattgatta tgtcgataca gaatggtgtc
     1381 ttagatgctt aaactatggc atattagttc atatcattcc tgatatagaa atggttcatg
     1441 ctattgggga taagtcagta aaaatctgtg ggattaacat accaattcac tcgccagtac
     1501 gtcgttatta tcgagtaaga aatgcatttc ttttgcttag aaaaaatcat gtgcctcttt
     1561 tactctctat tagggaagtt gttttttctt taattcatac gactttaatt atcgcaactc
     1621 aaaaaaataa aattgaatat atgaaaaaac atattttggc tacgctggat ggaataagag
     1681 gaattactgg gggcggtcgt tataatgcat agttctgatc aaaaaagagt agctgtactt
     1741 atggctacgt ataatggcga atgctggatt gaggaacaat taaaaagtat tattgaacaa
     1801 aaagatgtag acatctcaat ttttatatca
//
LOCUS       DAZPEU010000391         1823 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPEU010000391 DAZPEU010000000
VERSION     DAZPEU010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.398"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1166
                     /gene="rfaL"
                     /locus_tag="ACHU2L_004355"
     CDS             <1..1166
                     /gene="rfaL"
                     /locus_tag="ACHU2L_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR1358918.1"
                     /translation="AYWNRALVFLFITTYFLGGITRYKHLIVILMTITTIVYLCKRPK
                     HYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAIIENTLLCTIAIPVILR
                     DEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFADYRHRSISDSMVFLFP
                     ALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVIGLIWILMFKQWKLLL
                     VGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGSALDLILENPVIGYGY
                     GNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCAILKECI
                     KNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(1202..>1823)
                     /locus_tag="ACHU2L_004356"
     CDS             complement(1202..>1823)
                     /locus_tag="ACHU2L_004356"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1358919.1"
                     /translation="YQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          545 a          345 c          324 g          609 t
ORIGIN      
        1 tagcgtactg gaacagagct ctcgtattct tattcattac cacctatttt ttgggtggga
       61 ttacaaggta taaacatctt attgttattc ttatgacaat aacgacaatc gtctatctct
      121 gcaaacggcc aaaacactat ctctcactat ttaaaacatt tctttttggt agtgttgcca
      181 tattaactat tgctgcattg ctgtcacttc ttcaatcccc tgatgcaggt gctagcatga
      241 aggaagtttt caaagctatt attgagaata ctttactatg cacaatagca ataccggtca
      301 tattgagaga cgagaaaaga gaagatgtcg aaaaaatcgt tttcttctca tttattagtg
      361 cgttgggctt acgctgtttt tctgaattga ttacctatta taaggactat caacaaggga
      421 taatgccatt cgcagattat agacaccgta gcatttctga ctcgatggtc tttttattcc
      481 ctgcattgtt aaatctctgg cttatcaaat cagcaaaata ccgcatttct tttgtggttc
      541 taagcgttat ttttattttt ctgatattag gaactttatc cagaggggcc tggctttccg
      601 tgttagtcat tggattaata tggattctga tgtttaaaca atggaagtta ctattagtag
      661 gagtaatggt ttccatcatt gcattgtcgg ttattttcac acataaggag atgaccgcaa
      721 agctaacgta taaacttcaa caaactaata gttcttatcg ctatgcaaat ggtactcaag
      781 gcagcgcact cgatctaata ttagaaaatc ctgttattgg ttatggttac ggtaacgttg
      841 catataaaga tgtctataat aaacgtgtca ttgattatcc agaatggacc tttagacaat
      901 caatagggcc acataatttt gcgctattca tctggtttgg cactggttta ttagggctgg
      961 taagtcttat gatgctatac tgtgcaatat tgaaagagtg tataaaaaat ggcgtcaaga
     1021 ataaatatcg ctcaccatat aatgcatatt atataatctt actatctttt ataggttatt
     1081 ttgttatccg tggaaacgta gaacaaattg aaccaaattt attaggcgtt tacgccggct
     1141 tattattagc gatgaaaaac aagtaaaaaa taaaaaaggc tgcataatgc ggccttttta
     1201 tttattcaaa ccaattatga ataacctctt cgaatctttg agttacgcca tcccaactgt
     1261 agtgatcaaa cacaaagtcc tgaccttgct ttgcaactgc tgttaattca ggattcgcta
     1321 acgtttttaa aatatcgctg cttatggagt ctgcagtcat aggttctttt aaatggaatc
     1381 ccgtagtatt ttctttgaca aattccgtca tccccccacg cgtactcact aaaacaggct
     1441 taccagcacc catagcttca attgccacca tgcaaaatgg ttcctggaac tgggaaggaa
     1501 tgacgactaa atctgccagt gggtaataac aatacatctt ttcaggcggt atgcttccta
     1561 acataatgca acgatccttc aaacgttttg caatttccct gacattgcgt tgatatgcac
     1621 ctttgtcact tttactcatt tctgtataat caccaatcac aactaatttt aaatttttgt
     1681 gagcagcggc caatttttcg aatgattgca tcaacagcaa tatgcctttg tcagggacga
     1741 tacgcccagc ataaaaaata attttctctt caggagagat attaagttcg cttctagtga
     1801 tcggctgaaa attagattga tag
//
LOCUS       DAZPEU010000392         1821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPEU010000392 DAZPEU010000000
VERSION     DAZPEU010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.399"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..448
                     /locus_tag="ACHU2L_004357"
     CDS             <1..448
                     /locus_tag="ACHU2L_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1358920.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
     gene            798..1160
                     /locus_tag="ACHU2L_004358"
     CDS             798..1160
                     /locus_tag="ACHU2L_004358"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HFR1358921.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            1157..>1821
                     /locus_tag="ACHU2L_004359"
     CDS             1157..>1821
                     /locus_tag="ACHU2L_004359"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1358922.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFS"
BASE COUNT          534 a          368 c          421 g          498 t
ORIGIN      
        1 agagagtaaa tcaaaccgcg caattggcgt tgcgctgaat gatactgcat gtcgcgtatt
       61 gaaaaaacaa atcggaaatc atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa
      121 accagacgga acgaaagcgc caacagtaag gaagatgcgg tatgacgcaa acacagcctg
      181 gaaagcggcg ctgagacggg ctggtattga tgatttcaga tttcacgact tgagacacac
      241 ctgggcaagt tggctggttc aagccggagt cccgttatca gtgttacagg aaatgggagg
      301 ctgggagtct atcgaaatgg ttcgtcgata tgctcacctc gcacctaatc accttaccga
      361 acacgcacgg caaatagact tgattctgaa cccatcggtc ccaaatttgt cccagtcaag
      421 aaataaggaa ggtactaatg atgtgtaact tattgattta aatggtgccg ataataggag
      481 tcgaacctac gaccttcgca ttacgaatct gtagcaccaa tcataactat ctgttttagc
      541 aagcattaac cgcattcact aagatatagt tgatggcaca aacagaaagt tgatgaatga
      601 tgttgccata tatatgtcac aaatacggca caacgatctt caaacatata gccacacatt
      661 cacagaagag cacaaagcct tgcaatccag tgcaaagatt tgtgtgccag agtttttctc
      721 atcaactacc gcaagtatcg atcgattgag acttggatga tagacttcat gcctttgatt
      781 attagctgat agaagaaatg ttaaagctat ttgcaaagta cacatctata ggcgtgctga
      841 acacacttat acactgggtg gtttttggtg tatgtatcta tgccgcgcat accaatcagg
      901 ctatggcaaa cttcgcaggt ttcgttgtgg ctgtgagttt tagcttcttt gcgaatgcaa
      961 aattcacatt caaagcatca actacaacga tgcgctacat gttatatgtc gggttcatgg
     1021 gaacactgag tgctactgtt ggatgggttg ctgatagatg ttcacttcct ccaattgtca
     1081 ctcttgttac cttctccgcc atcagcttgg tgtgcggttt cgtctattca aagttcattg
     1141 tctttaggga tgcgaaatga agatatctct tgtcgttcct gtcttcaatg aagaagaagc
     1201 gataccaatt ttttataaaa cggtacgtga attcgaagag ttgaagccat atgaagtaga
     1261 aattgttttc ataaatgacg gaagcaaaga cgctacggag tcaatcatta acgctctggc
     1321 tgtttctgat ccgctagttg ttccgctgtc atttacacgc aactttggta aagaaccagc
     1381 attgtttgca gggttagacc atacaaccgg cgatgccgtg attccaattg atgttgacct
     1441 gcaagacccg attgaggtta tccctcatct tattgaaaag tggcaggcag gtgctgacat
     1501 ggtgcttgct aaacgttcag accgctcaac tgatggacgg ctgaaacgta agacagctga
     1561 gtggttctat aaattacaca acaaaataag caccccaaaa atagaggaaa atgtcggaga
     1621 ttttcgactc atgtctcgtg aggttgtgga gaacattaaa ctattgcctg agcgcaatct
     1681 tttcatgaaa ggcatactga gctgggttgg tggtcagaca gatgtcgttg aatatgtacg
     1741 cacagagcgt gttgctggca tctcaaaatt taatggctgg aaattatgga atctggcact
     1801 ggaaggtatc acaagttttt c
//
LOCUS       DAZPEU010000393         1817 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPEU010000393 DAZPEU010000000
VERSION     DAZPEU010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.400"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(53..1702)
                     /locus_tag="ACHU2L_004360"
     CDS             complement(53..1702)
                     /locus_tag="ACHU2L_004360"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1358923.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          419 a          489 c          478 g          431 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtca
//
LOCUS       DAZPEU010000394         1814 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPEU010000394 DAZPEU010000000
VERSION     DAZPEU010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.401"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..298
                     /locus_tag="ACHU2L_004361"
     CDS             <1..298
                     /locus_tag="ACHU2L_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358924.1"
                     /translation="SVSCFSDIPFVREHRIKLIMAGFLVYFWFFSFLVGWYKPQLKKE
                     YQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRFLIFNTEQSGFYEINDVYVRK"
     gene            532..660
                     /locus_tag="ACHU2L_004362"
                     /pseudo
     CDS             532..660
                     /locus_tag="ACHU2L_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            850..1254
                     /locus_tag="ACHU2L_004363"
     CDS             850..1254
                     /locus_tag="ACHU2L_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358925.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1317..1814
                     /locus_tag="ACHU2L_004364"
                     /pseudo
     CDS             1317..1814
                     /locus_tag="ACHU2L_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          509 a          404 c          450 g          451 t
ORIGIN      
        1 ttctgtatca tgtttttcag atatcccttt tgttagagaa caccgcatta agttaattat
       61 ggctggtttt ttggtttatt tttggttttt ttcctttctt gttggctggt ataaacctca
      121 attaaaaaaa gaatatcaga tgctttgtta taataatagt tggtattatg ttcttgctcg
      181 ttatgattca cggctcgttt tatctagttc atttaaagat gacagtaaca gattcttaat
      241 atttaataca gaacagtcgg gattttatga aatcaatgat gtttatgtca gaaagtaata
      301 atcctctccc acaaagtgaa ttaaatgaat atataatgcg ttcgaagtat atgtattgag
      361 ctgtcatact gagaaacaaa ccttagggca gcacaattaa agccagaaac aactatgcaa
      421 gaaagttact aaataagtgc tcggaacagt aaaacactca ggggggactc agtccggatt
      481 atgtaatcag ataaattgcc aaacatcaca aatcaccagc cggactgagt gatgccgagc
      541 attgcaccaa taccccgtga tgaacgatgc ctgatgcaga aagctcccta taaaacaaag
      601 aacaaaaact acgaccggcg attcaccgtc atactgatgc tgcatcgtgg tgacactgtc
      661 tgtcactttc atgttgactg aacaactctg ctgcgaagaa ggtgtaccgc cggcgggatt
      721 tgattgtccg gcgtctagct tccacttccc tgatggaact gaaagttgtt atggccggtc
      781 agcttgccct gtccgcagga catgaggtgc tgtgacggac agcaccagta acggaaagtg
      841 acaatgaaaa tgaatactgc gctgatagtt gcactaatgt gtatgtggta tgcggtaccc
      901 gctgctgcaa aagagacttt actggctatg ccacgtaatt caacagaaca ttgctatgcg
      961 gagattaatg tgcacggacc atatggggtg tatttccgag tggtcccgca tccccccggg
     1021 ggaaagagct gggtcgaatg taacagtgac tactattact ctgataaacc accgggagta
     1081 cagatactcg gaacccgtgc agggtgtcgg gtatacggaa tctgcgggac cacctcgaca
     1141 ctgcatgtgg ctggaagagg ggttgtgtgt attaaaaata tatgcagtcc acgtgggatg
     1201 ataattcaca ggatacggaa acgtcctgta gtggctgtaa gcgatgagat gtgatcttac
     1261 ccagcaatag tagacacgcg gctaagtgag taaactctca gtcagaggtg actcacatga
     1321 caaaaacagt atcaaccagc aaaaaaacac gtaaacagca ttcgcctgaa ttttgcagtg
     1381 aagccctgaa gcttgctgaa cgcatcggtg ttgctgccgc agcccgtgaa ctcagcctgt
     1441 atgaatctca gctctatgcg tggcgcagca aactgcagca acagatgacc tcgtctgagc
     1501 gtgaaagtga actgcccgcc tgaagcggca actggcggag caggcagagg aactggctat
     1561 cctccaaaag gccgcgacat actttgcgca gcggctggta tacgtggtgt cagcgtcgga
     1621 caggaataag ccctcgtcag cagttccgtc agcactgcga cagcgttgtc ctcgcggcgg
     1681 cctttacccg gtcaaaacag cgttacggtg caccacgcct gacggatgaa ctgcgtgctc
     1741 agggttacca ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag gggctgaggg
     1801 caaaggcctc ccgg
//
LOCUS       DAZPEU010000395         1809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPEU010000395 DAZPEU010000000
VERSION     DAZPEU010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.402"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            173..1198
                     /locus_tag="ACHU2L_004365"
     CDS             173..1198
                     /locus_tag="ACHU2L_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1358926.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHU2L_004366"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHU2L_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZPEU010000396         1803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPEU010000396 DAZPEU010000000
VERSION     DAZPEU010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.403"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            7..657
                     /locus_tag="ACHU2L_004367"
     CDS             7..657
                     /locus_tag="ACHU2L_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358927.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACHU2L_004368"
     CDS             629..1063
                     /locus_tag="ACHU2L_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1358928.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          509 a          356 c          412 g          526 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atc
//
LOCUS       DAZPEU010000397         1791 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPEU010000397 DAZPEU010000000
VERSION     DAZPEU010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.404"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            140..643
                     /locus_tag="ACHU2L_004369"
     CDS             140..643
                     /locus_tag="ACHU2L_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR1358929.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHU2L_004370"
     CDS             complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHU2L_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR1358930.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHU2L_004371"
     CDS             complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHU2L_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR1358931.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1438..1761
                     /locus_tag="ACHU2L_004372"
     CDS             1438..1761
                     /locus_tag="ACHU2L_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR1358932.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          464 a          429 c          397 g          501 t
ORIGIN      
        1 attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga ccggcagaaa
       61 ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct ctccacttct
      121 ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca actctcaaat
      181 gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt gttctgaaca
      241 gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg tgacccaaat
      301 gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta aagccattga
      361 tgttccaggt gaaaaactga actctctgga agaactgttc caaaaaacga tggaagaata
      421 cgagcaacgt tcaagcactc tggcgcagtt agccgatgaa gcgaaagaac tgaatgatga
      481 ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg gcctgttgct
      541 gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac
      601 cgaccaacat gttctgaatg tcgtgtcaca ccagctgcat tgatcataca tcggcgctaa
      661 tacattgcgc cgatgaagtt tttgagaaac cgctgcctca tctgtttgaa gcagcggttt
      721 ttttaatggg attcaccctg tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg
      781 attgcccgca tggtgaccgg gtctgcggca gcgggatgat gagtaaagtc gatattcttc
      841 agctgactgg acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga
      901 atttgtttga taaccaactg gcaagctacc acatcagaaa ccagttcctg atcggcattc
      961 agcggctggg acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc
     1021 acatcttcag cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag
     1081 gttttttgtt atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta
     1141 ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata
     1201 caggtgttat tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt
     1261 gatacaaatc acataaatac ccctttaatg ttataaaaat gataatcaaa aaacagcccc
     1321 cctatttctg acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca
     1381 ttgtcgcagc gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg
     1441 agacttctga ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccag
     1501 gcccatgtga gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct
     1561 tttttgcagc atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc
     1621 cagcaggaag gctataccca cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt
     1681 tttgcgggtt ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc
     1741 gccttccctg tctttctcta aatattttaa tcagcgaggg gatcttcgct g
//
LOCUS       DAZPEU010000398         1779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPEU010000398 DAZPEU010000000
VERSION     DAZPEU010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.405"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..360
                     /locus_tag="ACHU2L_004373"
     CDS             <1..360
                     /locus_tag="ACHU2L_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1358933.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACHU2L_004374"
     CDS             361..726
                     /locus_tag="ACHU2L_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1358934.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACHU2L_004375"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACHU2L_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACHU2L_004376"
     tRNA            1605..1680
                     /locus_tag="ACHU2L_004376"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACHU2L_004377"
     tRNA            1682..1758
                     /locus_tag="ACHU2L_004377"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
BASE COUNT          490 a          376 c          492 g          421 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtagg
//
LOCUS       DAZPEU010000399         1760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPEU010000399 DAZPEU010000000
VERSION     DAZPEU010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.406"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..264)
                     /locus_tag="ACHU2L_004378"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="ACHU2L_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHU2L_004379"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHU2L_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR1358935.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1164..1349)
                     /locus_tag="ACHU2L_004380"
     CDS             complement(1164..1349)
                     /locus_tag="ACHU2L_004380"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1358936.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1374..1646)
                     /locus_tag="ACHU2L_004381"
     CDS             complement(1374..1646)
                     /locus_tag="ACHU2L_004381"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR1358937.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          434 c          319 g          505 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg
     1441 gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgccg
     1501 ggttttctgg cttcataaga gtaagtgagc tggaaaacac gtttacatga gcgacaacgg
     1561 aaacgctcat gccgggaaca actacggcca tgtcgataaa cttcatcgga atgacagcgc
     1621 ggacaatgaa cagtaacggt agccatggaa aaccttaaat accagtagat taccagaaac
     1681 ttctgtcgat tgaagacatc acccgaagtt tgaattcatt cgaatacttt ttacgaggtt
     1741 cttttcgcca gtcctgtaat
//
LOCUS       DAZPEU010000400         1759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPEU010000400 DAZPEU010000000
VERSION     DAZPEU010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.407"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            301..495
                     /locus_tag="ACHU2L_004382"
     CDS             301..495
                     /locus_tag="ACHU2L_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358938.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            856..984
                     /locus_tag="ACHU2L_004383"
     CDS             856..984
                     /locus_tag="ACHU2L_004383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358939.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1294..1630)
                     /locus_tag="ACHU2L_004384"
                     /pseudo
     CDS             complement(1294..1630)
                     /locus_tag="ACHU2L_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          533 a          392 c          372 g          462 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat tgctttacga
       61 gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact gcaacaccgt
      121 atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga cccattgcga
      181 tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa aacacgtcca
      241 ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga ggaaaaccgg
      301 atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca acgggagttt
      361 gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt tccacttgaa
      421 gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt cttagaatgc
      481 aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg gcatcgttat
      541 cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct ttgtttgccg
      601 gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg acattgtaat
      661 aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat cgttgacaat
      721 atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct gaagcatccg
      781 acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg gaactttgac
      841 aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg gaaaagtggt
      901 acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag cttgtcagaa
      961 aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag tgcccggcca
     1021 gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc aggctatagg
     1081 caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac ggtctgaata
     1141 acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc gagcgctgat
     1201 tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa atataaaaag
     1261 atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc gtttaatatt
     1321 cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt acgaccacag
     1381 tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata agcagttgct
     1441 tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg atttccacat
     1501 tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc tgaagttctt
     1561 tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa cccactgtac
     1621 tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact ctatacagga
     1681 taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat ttctgacgaa
     1741 atcaacatga aaataacca
//
LOCUS       DAZPEU010000401         1750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPEU010000401 DAZPEU010000000
VERSION     DAZPEU010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.408"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..714
                     /gene="icsB"
                     /locus_tag="ACHU2L_004385"
     CDS             <1..714
                     /gene="icsB"
                     /locus_tag="ACHU2L_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR1358940.1"
                     /translation="GDNKTKDGKISHNLFGLDETNMSKFICKKKADAFRQLANYKLIS
                     KSENCAGMALNVLKAGNSEIYFPLPDVKLVATPNDVYAYANKVRQRIESLNQSYNEIM
                     KYIESDFDLSRLTQLRRSYLKSFNKINLIHTPKTFKPLSISLYKHPTENVSSEDFDAV
                     INACHSYLVKSAPSNMTRVLNELKTEATDKKEEIIEKSIKIIDYYNSLKSPDLGTKLY
                     IHDLLQINKLLLNNSHSNI"
     gene            727..1116
                     /gene="ipgA"
                     /locus_tag="ACHU2L_004386"
     CDS             727..1116
                     /gene="ipgA"
                     /locus_tag="ACHU2L_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR1358941.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            1132..>1750
                     /gene="ipgB1"
                     /locus_tag="ACHU2L_004387"
     CDS             1132..>1750
                     /gene="ipgB1"
                     /locus_tag="ACHU2L_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR1358942.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSN"
BASE COUNT          664 a          315 c          257 g          514 t
ORIGIN      
        1 ggtgataata aaacaaagga tggtaaaatc agccataacc tttttggcct tgatgaaaca
       61 aacatgtcaa aatttatatg caaaaagaaa gcagatgctt tcagacaatt ggcaaattat
      121 aaattaatca gtaaatctga aaactgtgct ggtatggcac ttaatgttct taaagcagga
      181 aattctgaaa tatactttcc actccctgac gttaaattgg ttgctacacc taacgatgta
      241 tacgcgtatg caaataaagt taggcaacgc attgaaagtc tcaatcagtc ttataacgaa
      301 ataatgaagt atattgaatc tgattttgat ctttccagat tgactcaatt acgacgcagc
      361 tatcttaaaa gtttcaataa aattaacctt atccacacac caaaaacatt caagccttta
      421 tcaatttcac tttacaaaca tcctactgaa aatgtatctt cagaagactt tgatgcggtc
      481 atcaacgctt gccactcata cttagtaaaa tcagcaccca gcaatatgac tcgtgtatta
      541 aacgaattaa aaaccgaagc aacagataaa aaagaagaaa tcatcgaaaa atcaataaaa
      601 attattgatt attacaattc tcttaaatcg ccagatcttg gaaccaaact ttacattcac
      661 gacttattac agatcaataa gcttttattg aataactctc attctaatat atagaaggcc
      721 atagaaatgt gtcgcaaact atatgataaa ctttatgaaa taacaggtgc aaagcttgat
      781 tttaacgata aaaatcaagc ctttatattg ctcgaagaac aaatacctgt ttgtataaca
      841 gataatgatg aatacatctt tttgaccgga cttcttaatg aacatgaact ttttacagaa
      901 aatattatta atccagaaca catactcatc cttaactact ctttgagtag agattatggt
      961 tcttctatct gtcttctccc tgatactcat caatgtgttt tgaccaagaa acactacaaa
     1021 aaatatcttt cacctgatga actcattgaa tctttgtatg aatttttatt ttgtattaaa
     1081 ctaaccattg caaacatcac ttcagaagtg aactaacata tagggggtat catgcaaatt
     1141 ctaaacaaaa tacttccaca ggttgaattc gcgattcctc gcccatcatt tgattcgtta
     1201 agtcgcaata agcttgtaaa gaaaatatta tctgttttta acttaaaaca acgctttcct
     1261 cagaagaatt ttggatgccc ggtgaatata aataaaatca gggacagcgt catagacaaa
     1321 ataaaagata gtaattcggg gaatcaacta ttttgttgga tgagccaaga gcgaaccact
     1381 tatgtctctt caatgataaa cagaagtata gatgaaatgg ccatacacaa tggagttgtt
     1441 ttgacctcag ataacaaaaa aaacatattt gcagctattg aaaaaaaatt cccggatata
     1501 aaacttgatg aaaaatcagc acaaacatct atcagtcata cagcactaaa cgagattgcc
     1561 tcatctggcc tcagagcaaa gattttaaaa cgctatagca gtgacatgga tttgtttaac
     1621 acacaaatga aggatcttac aaatctagta tccagttccg tctacgataa aatatttaat
     1681 gaatcaacaa aagttttaca aatagaaata agcgcagagg ttctaaaggc tgtataccgt
     1741 caaagcaata
//
LOCUS       DAZPEU010000402         1746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPEU010000402 DAZPEU010000000
VERSION     DAZPEU010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.409"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            453..1301
                     /locus_tag="ACHU2L_004388"
     CDS             453..1301
                     /locus_tag="ACHU2L_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1358943.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1318..1599
                     /locus_tag="ACHU2L_004389"
                     /pseudo
     CDS             1318..1599
                     /locus_tag="ACHU2L_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1635..>1746
                     /locus_tag="ACHU2L_004390"
     CDS             1635..>1746
                     /locus_tag="ACHU2L_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358944.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGK"
BASE COUNT          498 a          398 c          411 g          439 t
ORIGIN      
        1 gcattatata gttctactct ggcgagatac gttggcatat tcatttcctt actggttgtg
       61 tgagaacttc agtaaagata ccaccaaagc ccggaagtgg tgaaataaaa ccgggcacaa
      121 cacgaaggcg catttccggt atccataaag agtcggtctt gtctgtgaaa tttaaatggt
      181 gggagtgcgc ttccggttgt aaataacgac attgctgtgt gaagtaccag ttggcggcat
      241 cggtttaatt gctggctgat gtccgccctt tttaaagtga attttgtgat gcggtgaatg
      301 cggctatgcg cacgcggcac agttaaaagt catgttagtc cttattggtt tgggtgggaa
      361 agccaactgt aattgttaac tggttaacgt cacctggagg caccaggcac cgcatcaaca
      421 aagttcactt cggtgatgaa aggaaagaga aaatgttgaa tgtagctatt gaaaaccaga
      481 acgggtggaa ttatagtgca cctgcacctc ataaaacggg tgccgggatt gctaccccga
      541 cgatgactac tgcgcataac cgcgctcagg cggttttttt atgcgtcaag cacagccaca
      601 ttcagattat ggtggggcgt gcagggcagc cgcaaggctg gccggtttcg gtagtcaccg
      661 gttgtagcaa ccctgtgcgt ctcaccaccc atgagattgc tacctctggt ggtgagtcat
      721 ttaaactgac tatcgaggct gccatcatgg ctactatcct taccctttct caccctgacg
      781 caaccatcga aaatggtcgc gctgtcacta cgtctgttgc ggttgcggag ttcttccgga
      841 aaatgcacaa aaacgttatt cagaagatcg aaactctcga atgctcgccg gagtttaacc
      901 ggcttaattt taagcccgtc acctataccg acgccaaagg cgaaaaacgc ccaatgtacc
      961 aaatcaccaa aaacggcttc gttttcctgg tgatgggctt cactggcaaa aaagccgccg
     1021 cattcaagga agcctacatt gctgagttcg accgcatgga ggccgaactg cgccagaata
     1081 acaccccacc tgcagacaag atgattccgg gtgatggtcg cactctggtt gttcacttcg
     1141 acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg gtctgtaccc
     1201 tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac cggggcgcaa
     1261 ttaaaaatat gactgtggaa caattacttt ctttaaaata aaaatggagc tacggatatg
     1321 aaccctatgc ttgattattg ttttacctct gcacctcagc agaccataac acacaccgga
     1381 atcaaaaatt tgttatggat aatcaaaaaa aacctccagt agaaaggtga tcccgattta
     1441 attccctgtg cttatggcct gtgtgacaga caagtaagcc ctgataaggc ttacactgct
     1501 gaacttattt acatgcacgg aaccatgatg aggaaaaaga aacgcaaata ttgtagtgaa
     1561 atctgcgccg aaaaagacca gatggcacac gaactttaat taactgacta ttcgaaactg
     1621 aatttatgcc agcaatggca gggattcgct caaccttaat taaggagaaa aacatgaaca
     1681 ccaattatga agccactgtt gcaactactg ataacattgt tcacgaggtt tacctggaag
     1741 gaaagc
//
LOCUS       DAZPEU010000403         1744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPEU010000403 DAZPEU010000000
VERSION     DAZPEU010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.410"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHU2L_004391"
     CDS             complement(<1..353)
                     /locus_tag="ACHU2L_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1358945.1"
                     /translation="MNRHTQIRQSVLACLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="ACHU2L_004392"
     CDS             complement(350..940)
                     /locus_tag="ACHU2L_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1358946.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="ACHU2L_004393"
     CDS             complement(927..1298)
                     /locus_tag="ACHU2L_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1358947.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHU2L_004394"
     CDS             complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHU2L_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1358948.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          393 a          527 c          407 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcatgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DAZPEU010000404         1733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPEU010000404 DAZPEU010000000
VERSION     DAZPEU010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.411"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..143
                     /locus_tag="ACHU2L_004395"
     CDS             <1..143
                     /locus_tag="ACHU2L_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1358949.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACHU2L_004396"
     CDS             127..486
                     /locus_tag="ACHU2L_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358950.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACHU2L_004397"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACHU2L_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR1358951.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..1141
                     /locus_tag="ACHU2L_004398"
     CDS             707..1141
                     /locus_tag="ACHU2L_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1358952.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            1063..>1733
                     /locus_tag="ACHU2L_004399"
     CDS             1063..>1733
                     /locus_tag="ACHU2L_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1358953.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQINMQRRV
                     AWINP"
BASE COUNT          569 a          379 c          428 g          357 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatcaac
     1681 cttgaatggc aacaaataaa tatgcagcgc cgggtggcat ggataaaccc gga
//
LOCUS       DAZPEU010000405         1700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPEU010000405 DAZPEU010000000
VERSION     DAZPEU010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.412"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..1700
                     /locus_tag="ACHU2L_004400"
                     /pseudo
     CDS             1..1700
                     /locus_tag="ACHU2L_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT          569 a          323 c          321 g          487 t
ORIGIN      
        1 tgttttataa agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa
       61 aaagcttctc agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg
      121 tcattaaatt ttccacgtcc gtcttacaag ctgataagaa agaaattacg atgattaata
      181 aaaacgaaaa cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg
      241 aaattttacc tcaacgctca gacactcttg ataaacaaga attaaaccaa aaatcagata
      301 gaaaagaaaa agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc
      361 ccaaaaccgt atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa
      421 tgttgattac aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt
      481 caacaaaaaa tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg
      541 cgttgctgcc tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc
      601 aaatggcatc agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg
      661 gctaagaata aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc
      721 taatgtaact aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta
      781 ttctctgatg gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt
      841 tattaaccaa tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa
      901 aggaactgtg gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc
      961 aattggtcag aatcaccaac ttacgcaact gatgattcag ctccagaaaa tgccagaact
     1021 acatagaaca gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc
     1081 tataaattat ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg
     1141 atatgaaggg ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa
     1201 tggtttttct ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat
     1261 tctgaacgtc ttacctaaac tggccgcaac acatcattta gataatgaac aggtgtataa
     1321 attcctgagt gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg
     1381 tgatgccgac atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca
     1441 tttgactaaa gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag
     1501 actatacctg gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt
     1561 gccctgtctg gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc
     1621 taaaagtctt gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt
     1681 tattaatact atttttaacg
//
LOCUS       DAZPEU010000406         1673 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPEU010000406 DAZPEU010000000
VERSION     DAZPEU010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.413"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(6..509)
                     /locus_tag="ACHU2L_004401"
     CDS             complement(6..509)
                     /locus_tag="ACHU2L_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1358954.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(554..1517)
                     /locus_tag="ACHU2L_004402"
                     /pseudo
     CDS             complement(554..1517)
                     /locus_tag="ACHU2L_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          475 a          361 c          340 g          497 t
ORIGIN      
        1 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
       61 cacaaggcat aaaacatcat taccactcca cttaatggca gaatgatata tacgtatccc
      121 atcggtattt gcatagcggc agatacctgt gatgttgcca gtgttttatc aattaatttc
      181 aggccaccag tcacaatgac cgaaccggca aaacctaaaa tgagaacgtt aatgactata
      241 ctaagaagta attgtttgcg cttattcagg gcgagagcaa ataaatcaat agagagatgt
      301 cgctgcgcgc cgacggtata tgcagcacct aataatccta cccagatcat caaaaagcgt
      361 gccagctcat cggttaatgt acttggctgg ttaagtacgt agcggctaaa tacttgccag
      421 actacgcata caaccagagc aagcattacg gcgacagaaa acgtggcaat gacgcggtct
      481 accgcaagtt tcaggcggtt aaatatcatt gtaagcatcc tggaaaatca gggccagagg
      541 ctggcccgga atattattga gcagcgttgt cgaatttctc taacaaagca gcttgttttg
      601 gatctttttt gaagtcgtca aacagaggtt gcacggcttt acggaatggc gctttatcca
      661 cttttacaat ttcacccccc atagctttcg cctgagcacg tgtgtcagcg tcaatttttt
      721 cccacaattt ttgttgataa gcttcggatt tcttcgccgc tgtttcgagg atttgttgct
      781 gttctggggt taatttattc catgttttgg tggagataac caaaaagtca ggaattgagg
      841 cgtgttcatc ttcagaaaaa actttggcaa tttcaatatg tcgagtctgc atccaggaag
      901 gcacgttatt ttctgcgcca tctactacac cttgctgcat cgcagtataa acttcgccaa
      961 atgaaattgg agtgggtgaa ccacccatca gttcaatcat tttgatggtg gtcggacttg
     1021 gctgaacacg aattttcagt cctttaagat cttctggctt tgtaatcggt tttttcgcat
     1081 agaaactgcg agtacccgct acataagctg ataaagcaaa aaagcctttg tcttttgttg
     1141 agtccataat ttctttacca acttcaccaa aaacaactta ataagaacgg caaattatag
     1201 atagcataaa tattatcaaa agactccaga tcactggcgg aacctttggt catatccagt
     1261 gcgccatttt gcaacaattc catagtttct cgcgcattac ccatctggct gctgggataa
     1321 atacggatca ccatattacc ttttgacagc tgttttacct ctttcgccag ctcttcaaaa
     1381 ctctgatgta caacatgact tctttccagg ttgtgtgcta actttaacgc cactttttct
     1441 gcggcgttta cgccagtgct aaccagagcc agcaaggatg cgcagactaa tgagcgggta
     1501 aatgacctca ttttcataaa aaaactccct gttgaaggat ggaaattggt tgatatctaa
     1561 ttgtgcttat cagtgatttg tgcgacaaat tggttaacct gtctgacaac ttgtctatgt
     1621 ttatcacaac ttgaaataaa gtcagaaatt aaatattaaa tttgtgcgcc agg
//
LOCUS       DAZPEU010000407         1665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPEU010000407 DAZPEU010000000
VERSION     DAZPEU010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.414"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(8..211)
                     /locus_tag="ACHU2L_004403"
     CDS             complement(8..211)
                     /locus_tag="ACHU2L_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358955.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            289..1383
                     /gene="ddlA"
                     /locus_tag="ACHU2L_004404"
     CDS             289..1383
                     /gene="ddlA"
                     /locus_tag="ACHU2L_004404"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1358956.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1407..1619)
                     /locus_tag="ACHU2L_004405"
     CDS             complement(1407..1619)
                     /locus_tag="ACHU2L_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR1358957.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
BASE COUNT          445 a          399 c          400 g          421 t
ORIGIN      
        1 ttaattatca acctttatta taaataaaag ctatgaaata tattctcttc gttaacttat
       61 aattaagaga aaaaaactca ccgcattgcc atttaagcca ggaataaatt agcttcccgt
      121 tcatcatttt tgtgatcttg cgcacatctt tgagataacg cactttcttc accagtacgt
      181 tgcacgcttt gtcagcagaa gattgcaaca atctttcaga aggccgcgaa tcccccgttt
      241 tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat ggaaaaactg
      301 cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct gcaatcggca
      361 aaaaacattg tcgatgccat tgataaaagt cgcttcgacg ttgtgctgct gggcattgat
      421 aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc agacgatcct
      481 gcccatattg cgttgcgccc ttcggcgacc agccttgcgc aggtgccagg taaacatgag
      541 catcagctta tcgacgcgca aaacggtcag ccgttgccga cggtggatgt cattttcccg
      601 attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg ggtcgccaat
      661 ttaccgtttg taggttctga tgttctggct tcagcagcct gtatggataa agatgtcacc
      721 aaacgtctgc tacgcgatgc cgggctgaac attgcgccat ttattaccct gacgcgcgct
      781 aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc actgtttgta
      841 aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga agaacagtac
      901 gcaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga gcaagggatc
      961 aaaggtcgtg agatcgaatg cgcagttctg ggcaacgaca atccgcaagc cagcacctgt
     1021 ggcgagatcg tactcaccag cgatttctat gcctacgaca ccaagtacat tgacgaagat
     1081 ggcgcgaaag tggtagttcc ggcagccatt gcaccagaga tcaacgataa gatccgggcg
     1141 attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt agacgtgttt
     1201 ttaaccccag agaacgaagt ggtgatcaac gagatcaaca cactgcctgg cttcaccaac
     1261 atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga tctgatcaca
     1321 cgtttgattg agctggcgct ggagcgtcac gctgcggata acgcattgaa aaccacaatg
     1381 taatatttta acgcccgata atattcttat tgggcgtttt cagtttcttc atcgcgacga
     1441 cgaataatca accccgccag ccagaaacta atcacccacg tcaccagacc gacggcatag
     1501 gtctgccaac cttttgcttc aaatcccagt acccccacca cgccattaag aatgaatata
     1561 aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg cagattcatg
     1621 gcagctccgt taacgaaaaa caacatcctt gcatattctg taaca
//
LOCUS       DAZPEU010000408         1650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPEU010000408 DAZPEU010000000
VERSION     DAZPEU010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.415"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..325)
                     /locus_tag="ACHU2L_004406"
     CDS             complement(<1..325)
                     /locus_tag="ACHU2L_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358958.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELL"
     gene            complement(909..1310)
                     /locus_tag="ACHU2L_004407"
                     /pseudo
     CDS             complement(909..1310)
                     /locus_tag="ACHU2L_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1343..1650)
                     /locus_tag="ACHU2L_004408"
                     /pseudo
     CDS             complement(1343..1650)
                     /locus_tag="ACHU2L_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          481 a          384 c          318 g          467 t
ORIGIN      
        1 ttaaaagttc gaatattaca gggaaaaaca gtagggtaat agcaaaaaat agtctaaaaa
       61 aactcaagtc ttgtatttta cttcttttct ttataagaag aaaaaataaa ataccaatcc
      121 cccaaccggt aaaaagtata cccattacta tgatagcata gagtaaactt ctggcaacat
      181 catccaaacc tgcaccaaca acccaccatg gaaatccata gtaaaaagag gttccccagc
      241 cataagaata agcactgacc caccctacac atcccatata ggcgatgaat aatgaagaat
      301 ttctaagcaa tgaattgtca ttcataagaa actaactgat aatgtaaaaa atattcgaga
      361 taaaagttga tcttttaatt ttcatgctag gaaattaaca ataatatcat tgttaatgca
      421 tatttatcat atcaaaatac tctgcagaat gctactatca aaaccagaca agtgaacaaa
      481 acttggtcaa catttagtaa cgctagttgg taatgtcttc aatcgataga agtttctggt
      541 aatctactgg tattcaaaca gttagggctc atacagcttg ctggaccttc cccataaccg
      601 cccgaacgtg aaggtctgat gtgttgcaag agagccggat gcgggaaatc tgcaagtccg
      661 gttcgatgag agggacgttg tttttacttg ttgttgatga agactttcat tattgaacag
      721 aatgataata ttgtctaaac aaaacattaa atctcataat tctttgtaga aaacgctttt
      781 cttgaaatta aaaataagta gaatcagcag tctcattata ataacagaca atgtcatgct
      841 ctcgcccgtt ccggtaataa acggagcggg ctatcttttt ggaatattta tggcccacaa
      901 tccagttttc ataaatacga ttcattccat tcttcgtttt ttctgagcat tgcgttcata
      961 atggtcagca acttacgcat acaggcaacc agtgccactt ttttggcgtt tccggctgca
     1021 agtaggcgca tgtaaaacgc ctttatcacc agattaaacc gggggggggc ctcaagcgcc
     1081 gccatgtaca gagctgttct tccccagcac aatcaccgaa aatggttcgt cgaccgcgca
     1141 gagttcccga gtcccggttt acctgagcaa caccgacaag tgcgctgatt tcacgtctcg
     1201 agaggctgct cgattcaggc atttccgcca gcaatgctgc ggcagtcatg gctctaacac
     1261 ctcagacgtt cagtaagctc tttaaagtaa ctctgaatgt gcctgttcat atctggggct
     1321 tccccgatca tggtgggaag gcttagaacg ctatattcag tttcccatag tggaacatca
     1381 ctcccagctt aaagcgctcc cggttgcggt agcccttggc ttttattctc agcagcctga
     1441 tcttgctgtt cagtgcttcc gcatttccgt ttgatacgcc atggcgcatg gcgttcagga
     1501 taccgtacaa ccttttccct atcgttttag ccacattctt catcatgggg acgtcactgt
     1561 tggccgcagc catctctgcc agtcgttgcg tctttcctcg ctccatggtc tgctccagat
     1621 atcctttgcc agttctttca gcgcccagca
//
LOCUS       DAZPEU010000409         1645 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPEU010000409 DAZPEU010000000
VERSION     DAZPEU010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.416"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(72..148)
                     /locus_tag="ACHU2L_004409"
     tRNA            complement(72..148)
                     /locus_tag="ACHU2L_004409"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Arg,seq:tct)
     gene            complement(150..225)
                     /locus_tag="ACHU2L_004410"
     tRNA            complement(150..225)
                     /locus_tag="ACHU2L_004410"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Ile,seq:cat)
     gene            complement(402..1091)
                     /locus_tag="ACHU2L_004411"
     CDS             complement(402..1091)
                     /locus_tag="ACHU2L_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR1358959.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1088..1447)
                     /locus_tag="ACHU2L_004412"
     CDS             complement(1088..1447)
                     /locus_tag="ACHU2L_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1358960.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1447..1635)
                     /locus_tag="ACHU2L_004413"
     CDS             complement(1447..1635)
                     /locus_tag="ACHU2L_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358961.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          394 a          462 c          352 g          437 t
ORIGIN      
        1 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc
       61 taagtcggcg ctggcccacc accgaggact cgaacctcgg accgtcaact tagaaggttg
      121 atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac
      181 ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata
      241 accatatgtc atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc
      301 atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac
      361 aaaatcggca aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata
      421 ctttgctttg aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc
      481 tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg
      541 gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc
      601 ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt
      661 tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc
      721 cagtgggtta tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac
      781 caggctttat agtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac
      841 gcaacctgac tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt
      901 ggtggattat cccgcgttat catttttccg gtcacctcat cgcggtaccg gattttttta
      961 cgcctgtaac gccctgtatc gaacatggca ttctcctgcc aggcttcaag ctgacctttt
     1021 gtcgccccac tcaaatcagc ggtggcgata atgagctgct cacgaacaaa ctgtaaatac
     1081 tggttattca tgcacactcc agctctgtga tttttatccc caaccgacca ccaggaacag
     1141 gcagtccgcg cacaatattg gtttcatcaa actgctcgtc gtcgataagc aaccccgcat
     1201 gtgtcagtgc atccagtggt gctttcagga tattgtccag gtcacgacgc cgcttatccg
     1261 gtggctctgc aataatcttt atcgccagtc ttccggacag gcttaatttc agtcgctgct
     1321 gacggacaat aagcgccaca tcccggcgat aacgctcacc ggcttttgat acaaaatatg
     1381 tgctgccacg acgtcgccag taagtattca ccgtcggcgg ataaggtaaa ataaactcat
     1441 ggcgcatcag cgcagcacct cctgcaccag cttttcaaac tttccgactc tggtttccag
     1501 ctctgccaca cagtccacca gctcatctac tgctttctgt gcgcgatgct tcgcctgcat
     1561 cagttcccga agcgcgggta ccatattgag gtagcctgag tttaacggac actccttcct
     1621 gaaatagaat ggcatcagaa ggagc
//
LOCUS       DAZPEU010000410         1631 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPEU010000410 DAZPEU010000000
VERSION     DAZPEU010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.417"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(38..481)
                     /locus_tag="ACHU2L_004414"
     CDS             complement(38..481)
                     /locus_tag="ACHU2L_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1358962.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            638..1567
                     /gene="metA"
                     /locus_tag="ACHU2L_004415"
     CDS             638..1567
                     /gene="metA"
                     /locus_tag="ACHU2L_004415"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR1358963.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          392 a          462 c          363 g          414 t
ORIGIN      
        1 cgtcttctcc agcttgggca ggcacggcag aggcggctta ttcccccaca tacgccagat
       61 tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta accttaaaac
      121 ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg gttgtcagtt
      181 ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag ccgcacacat
      241 caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac ccaaccggct
      301 gctcccgttc attaaccgcg acccacaacg gtgcttccgg cagaaaggaa cgcaccagct
      361 cttccagctc ggcccgatac tctgctgata aaaaatcgtg ggtggcatcg acagatcgac
      421 accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata ctaataacca
      481 ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga aactttctca
      541 ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct atctggatgt
      601 ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg tgccggacga
      661 gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa cttctcgtgc
      721 gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc cgaagaagat
      781 tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg tcgatattca
      841 gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc tgaacaactt
      901 ctactgtaac tttgaagata ttcaggaaca gaactttgac ggtctgattg taaccggtgc
      961 gccgctgggc ctggtggagt ttaatgatgt cgcttactgg ccgcagatca aacaggtgct
     1021 ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg tacaggccgc
     1081 gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct ctggcgttta
     1141 cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg attcattcct
     1201 ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg attcgtgatt acaccgatct
     1261 ggaaattctg gcagagacgg aagaagggga tgcatatctg tttgccagca aagataagcg
     1321 cattgccttt gtgacgggcc atcccgaata tgatgcgcaa acgctggcgc aggaatattt
     1381 acgcgatgtg gaagccggac tagacccgga tgtaccgtat aactatttcc cgcacaacga
     1441 tccgcaaaac aaaccgcgag cgagctggcg tagtcacggt aatttgctgt ttaccaactg
     1501 gctcaactat tacgtctatc agatcacgcc atacgatcta cggcacatga atccaacgct
     1561 ggattaatct tctgtgatag tcgatcgtta agcgattcag caccttacct caggcacctt
     1621 cgggtgcctt t
//
LOCUS       DAZPEU010000411         1628 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPEU010000411 DAZPEU010000000
VERSION     DAZPEU010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.418"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(64..1160)
                     /locus_tag="ACHU2L_004416"
                     /pseudo
     CDS             complement(64..1160)
                     /locus_tag="ACHU2L_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            1375..>1628
                     /gene="yfhL"
                     /locus_tag="ACHU2L_004417"
     CDS             1375..>1628
                     /gene="yfhL"
                     /locus_tag="ACHU2L_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR1358964.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHAD"
BASE COUNT          420 a          392 c          398 g          418 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcattttaca gccccgtccg ggctgtatgt tttcaccagc ttatccagtg atcgttttga
      121 aaatggctgc attggatcac gtggctgcaa aaatacataa tccctgttgc actcaggaac
      181 tgaatcacgc caggctttct gctcgtcgat aatccgccgg atctcaggcg ttatcggcag
      241 gcggaaagcc ttttgtgttt tcatagcccc tcgcatgccg ataacccctt ccggataaac
      301 aatttcacca gcctcctcgt aaacgtagtc ccagcgcagg ttatggacat taatcggacg
      361 aacaccagtg atgatcatga agcgaacagc attcttctgg tgtacagagg tgcaggcagc
      421 aacattgagc cagagtcggg cgattgattc aatatcggta aaaagccgtg tgggggtagg
      481 tttctgtacg cgggaggaaa cataatcatc tggcagactg gcggcaacat tgcggccgtt
      541 gcaaagagta ggtgcgcaga acttccagaa ccgacggagc tcggcaaaca actccagggc
      601 gttattgttc gagcgagtgg cgatccactc gtccagcact tccaccagcc gattgtatgt
      661 tacgtcgctg aacacctcac gctcgccgaa cgttacttta atccggtcga tacgcacccc
      721 gtaggttgtg aaactgtccg ggctcagctt ctgccgggct actttggctt tgggtcatcc
      781 cggtacattt ccagcgctgc atgtacggac tctgcccgca gtccaccgtc agccatacct
      841 agtgcttttt cgcgcgccag ctggataacg agctccggcc actcgccgag ctttttacct
      901 ttgaggccca tcttttttgg aaactcggcg taaaatgtaa ccttaccggc tttgctgaaa
      961 tcgatacgga gatagttctc tttttcgtat ttggaacagc gagccacgcc ggaagcagcg
     1021 aggatgattt tggcggcagc aacacagatt ttcatgtgtg cgctggtata ggggggttta
     1081 caggcgtccc atttttcaga tgcggctaaa acatcgtcat tattggggct atccggatta
     1141 tgtgttacag tgcgcggcat tctcaatcct tatctgcgta ggcgcagaaa acaagctcac
     1201 acatacaagt cttttctacg ggacaaaatg caatgtgttg cggttttgtg ttgctggact
     1261 gagtttatca aggttaaata cactggatca acatacagta agttaatgac agtaaagcat
     1321 acaaactcga tacaacttac tgattttaaa atgatttaac ggtaatccat tgaaatgtct
     1381 ttactaatta ctaaacgctg tattaattgt gatatgtgtg aacccgaatg cccgaatgag
     1441 gcgatttcaa tgggagatca tatctacgaa attaacagcg ataagtgtac cgaatgcgta
     1501 gggcactacg agacaccaac ctgccagaag gtgtgcccga tccccaatac tattgtgaaa
     1561 gatccggcgc atgtcgagac agaagaacag ttgtgggata aatttgtgct gatgcaccac
     1621 gcggataa
//
LOCUS       DAZPEU010000412         1606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPEU010000412 DAZPEU010000000
VERSION     DAZPEU010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.419"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1076
                     /locus_tag="ACHU2L_004418"
     CDS             <1..1076
                     /locus_tag="ACHU2L_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1358965.1"
                     /translation="VTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGD
                     KTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGSL
                     DYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDFFGLIEGLNF
                     AAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERA
                     AANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNKAQSFEVVAQ
                     YQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYVDYKINLIDE
                     SDFTRAVDIRTDNIVATGITYQF"
     gene            1240..>1606
                     /locus_tag="ACHU2L_004419"
     CDS             1240..>1606
                     /locus_tag="ACHU2L_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1358966.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITF"
BASE COUNT          475 a          340 c          334 g          457 t
ORIGIN      
        1 ttgtaactgg tttgttagct gcgaacgtag cacacgctgc cgaagtctat aacaaggatg
       61 gtaataaact cgacctttat ggcaaggtta ccgctctacg ttattttact gatgataagc
      121 gtgacgatgg tgataaaact tatgcccgtc tcggctttaa aggagaaacg caaatcaatg
      181 atcaaatgat tggttttggt cactgggaat atgattttaa aggctataac gatgaagcca
      241 acggctcgcg cggcaacaag acccgtctgg cctatgctgg tttaaaaatt agtgaatttg
      301 gctctctgga ctatggtcgt aactacggtg tcggctacga cattggttca tggaccgata
      361 tgttgccaga atttggtggc gatacctgga gtcagaaaga tgtcttcatg acataccgta
      421 ccaccggtct agcaacctat cgcaactacg atttctttgg cttaattgaa gggctgaact
      481 ttgccgcgca atatcaaggc aaaaataaac gtactgacaa cagtcatctt tatggtgctg
      541 acgacacgcg tgccaacggt gacggtttcg gtatctcctc aacttatgtt tatgatggct
      601 ttggtatcgg tgcggtatat accaaatccg atcggacaaa tgcgcaggaa agagccgcag
      661 ctaatcctct caatgcctcc ggtaagaatg cagaactatg ggccacaggt ataaagtatg
      721 atgccaacaa catctacttt gcagctaatt acgatgaaac attaaacatg accacctatg
      781 gggatggtta tatctctaac aaagcacaaa gttttgaagt ggtggcgcaa tatcaattcg
      841 acttcggctt gcgcccatca ctcgcttacc tgaaatcgaa aggcagagat ctgggccgct
      901 acgccgacca ggacatgatt gagtatatcg acgttggtgc gacgtatttc ttcaacaaaa
      961 atatgtcgac ctatgttgat tataaaatca acctgattga tgaaagcgac tttacccgtg
     1021 ccgtagatat tcgcaccgat aacatcgtcg ctacgggcat tacctatcag ttctaacgtc
     1081 ctctcctgac cagcccttct ctgggctggt ttttgattta agtgctgctg tatgactcct
     1141 taagtaaaaa tgagcaattc ttgatatttc tggtcattca cctcaattaa tattattcca
     1201 tatcctccgg ggacatctta ctataatatt tattgatgag tgattatgac caatattaat
     1261 acagcttgtg taaaaaataa tgccagttat caacttaata acgcattacc taacaaagaa
     1321 accatctcca gcaatttttg tgaacgacta gaacaatggg gtaataaatc ggttaataat
     1381 ggtgaagaaa gagcaattgc cgtggagcga attaaagaag cttacaattc gaacatggca
     1441 tctcttgatt tgtcatatct tgatttgagt gaattaccgc ctatcccctc gacagttaat
     1501 acgttaaatc tggaaaacaa ttgtctaact tgtcttgact tagctgacaa tgccagtctc
     1561 gttaatatca acctcagcgt taataaaatt aacacgataa cctttc
//
LOCUS       DAZPEU010000413         1603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPEU010000413 DAZPEU010000000
VERSION     DAZPEU010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.420"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(335..481)
                     /locus_tag="ACHU2L_004420"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="ACHU2L_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="ACHU2L_004421"
     CDS             535..717
                     /locus_tag="ACHU2L_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR1358967.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="ACHU2L_004422"
     CDS             complement(818..1147)
                     /locus_tag="ACHU2L_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR1358968.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1603)
                     /locus_tag="ACHU2L_004423"
     CDS             complement(1319..>1603)
                     /locus_tag="ACHU2L_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358969.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          377 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa atg
//
LOCUS       DAZPEU010000414         1581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPEU010000414 DAZPEU010000000
VERSION     DAZPEU010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.421"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHU2L_004424"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHU2L_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR1358970.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACHU2L_004425"
     CDS             complement(1269..>1581)
                     /locus_tag="ACHU2L_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR1358971.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZPEU010000415         1561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPEU010000415 DAZPEU010000000
VERSION     DAZPEU010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.422"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            400..1149
                     /gene="phoC"
                     /locus_tag="ACHU2L_004426"
     CDS             400..1149
                     /gene="phoC"
                     /locus_tag="ACHU2L_004426"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR1358972.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1204..1308)
                     /locus_tag="ACHU2L_004427"
                     /pseudo
     CDS             complement(1204..1308)
                     /locus_tag="ACHU2L_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1319..>1561)
                     /locus_tag="ACHU2L_004428"
     CDS             complement(1319..>1561)
                     /locus_tag="ACHU2L_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1358973.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          426 a          332 c          316 g          487 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta tttttcagct
     1561 c
//
LOCUS       DAZPEU010000416         1488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPEU010000416 DAZPEU010000000
VERSION     DAZPEU010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.423"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..89)
                     /locus_tag="ACHU2L_004429"
     CDS             complement(<1..89)
                     /locus_tag="ACHU2L_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1358974.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEG"
     gene            complement(157..639)
                     /locus_tag="ACHU2L_004430"
     CDS             complement(157..639)
                     /locus_tag="ACHU2L_004430"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1358975.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(630..932)
                     /locus_tag="ACHU2L_004431"
     CDS             complement(630..932)
                     /locus_tag="ACHU2L_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1358976.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1204..1476
                     /locus_tag="ACHU2L_004432"
     CDS             1204..1476
                     /locus_tag="ACHU2L_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358977.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          425 a          370 c          317 g          376 t
ORIGIN      
        1 ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc
       61 gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca
      121 ggaaggagtg tccgttaaac tcaggctacc tcagactcag actttataaa acaaggtatt
      181 aggtgccaca actgactgaa caaaaccaaa acgctcataa aatgctctgg cgtcatcatc
      241 aattgcatgt accataacag ccttaatgcc aacctgatca gccagattga caacccggtg
      301 aatggcatca cttagtagcc atttaccgaa accatgcccc tgggtacaaa catcaacagc
      361 cagacgccct aacagaacaa cagggacagg attgggcatg ttatgccgca aactgcgacc
      421 aagctcggca tgagtgacgg aaccagtagc taatgagtag tatcccacaa tacgtaaagt
      481 atcctccaag caaataacga acgtgcgaga agcattgagc atctggtttt tcatagcccg
      541 accacgtaac cagttactta gcacctcatt tccacagtca aatggctgaa gtatatgctc
      601 atccgtcaac aatgctggcg cggtgacatt tatttccatt caggctttac cgccatcaga
      661 cgttcacgcc cttctgcatt ctgcacagga gcttccagtt gcgtaataaa cgaatcataa
      721 cgttcgttat ccagcacaaa taaacgttga tccaggataa tgtttttagc ttcctggtac
      781 gccagttcca tgatgaaatc ggtacggttc ttattgacca agctcgcagc ataatcaata
      841 acggctcgtt cctcatcagt tgcccggatg ttaatctgat gttctcgcgg tgttttacgg
      901 acgcttgcag ctgtagacat aaaaacctcc attggtaaca atttgctatg caattaatgt
      961 tatcgcaaat ggtaacagaa cgctatacag aatcacttac gggaaaagcg atcccctgat
     1021 aaaaacaatg gcagagacct gaacattgtg acgatgatct gtgtcggcgc acaaatcatg
     1081 tctgattacg gccgtttaat gtgattttga taatgaaatg cctgaaccac ggcagcctgg
     1141 cggctgcaaa tcggggtgtg gcagaatatg agctttcaga gtaaccatcc agaatgagtt
     1201 cccatgtcac acaacctgga gcaccagaaa gtccacacca gaatggtgaa agaggttctg
     1261 aaagccgtcg ccagagccaa taatcaccct tataagtccg tatttgcaga ctttatcacc
     1321 ggtcatccct cctgcacggt ctgtttctgg gaaaccttcc acaaaatgta cccagattca
     1381 ccgtacgaat acgtcacgtt ctgccatacc tgccgtcgtt ttgatttata cgaaacagaa
     1441 gcggagatga aggcggatga cccgaagtgg tggtgatttt cagccccg
//
LOCUS       DAZPEU010000417         1467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPEU010000417 DAZPEU010000000
VERSION     DAZPEU010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.424"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..464)
                     /locus_tag="ACHU2L_004433"
     CDS             complement(<1..464)
                     /locus_tag="ACHU2L_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR1358978.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPV"
     gene            778..>1467
                     /gene="icsB"
                     /locus_tag="ACHU2L_004434"
     CDS             778..>1467
                     /gene="icsB"
                     /locus_tag="ACHU2L_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR1358979.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKI"
BASE COUNT          494 a          290 c          217 g          466 t
ORIGIN      
        1 tcgaccggtc cccaattctt attatttaaa taatcagtaa atatttgttt tattttcgtt
       61 tgcaattcct ttttattttt aatgcctagc tgtgctctta tctcttcact tattacttta
      121 ttaagctcat taatatgtct tttattaact tcaccaagtt tataggtatc taatcgttcc
      181 atcatggctt taatttttga cgtatcacca cctgccaact ttattgcctc gcaaaaaact
      241 tcagctctgt cacgcgcaaa caaaaggctt tttcctgtta agcttgtttg gttctgtaaa
      301 tataatcgat taagcgcttc gatcccctta tttctttctc catttttaac tctttgatat
      361 gaaatcacgc ttacaccttt gtgctttccg gtttcttctg cgcctttata ggaatctccg
      421 ctttgaaatg aaacctgatg caatcccaaa ttagttatgt gcatttaatt atcctcagtc
      481 ataaatgggg cattgattgc agttttgtac ctcgttttac aacaaaaaag atacttattc
      541 aacagggttc tctctttttc tataacactc cgagcctgat acatcaggca ctttatcttg
      601 tgggatttca tgatgaaacg agcactacat atgctcacga ggtacatatt atgtacagca
      661 aaagaaaatt tgattatgtt aacaggctca agtttctaaa tttattaatt gaatatatta
      721 tataccaaat acattttaca tccccacaat caccaagtaa tggagagtta ataaagtatg
      781 atcctcaaaa ttagcaattt cattgacgca agcaatacaa aagggcctat acgcgttgaa
      841 gatacagagc atggacctat attgatagca cagaaattca acctaaagga tctctttttc
      901 agaacattaa gcactattaa tgcaaagata aattcacaga tattaaatga acaactaaaa
      961 aattatcgac tagagaatca aaaatcctta ttgcttttcc tcaatacatt agctagcgaa
     1021 aaatctgcag aaagtgcgtt tgccgcctat gaagcagcta aaaactcaat tcaacactct
     1081 ttcacaggta gagacatcaa attaatgttg aataccgcag agcgttttca tggcatcggt
     1141 acagccaaaa atcttgaaag acatttagtt tttcgttgct ggggaaatag aggcataacc
     1201 catttagggc atactagtat cagtataaaa aacaatttac tacaggaacc aactcatacc
     1261 tatctcagct ggtatcccgg cgggaatgtt acaaaagata cagaaataaa ctaccttttt
     1321 gaaaaacgct caggttacag tgtcgatacc tataagcaag acaaactaaa tatgatttca
     1381 gaccaaacag ccgaaagact tgatgcaggc caagaggtta gaaacttatt aaattcaaaa
     1441 caagatcaaa ataacaacaa aaaaata
//
LOCUS       DAZPEU010000418         1457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPEU010000418 DAZPEU010000000
VERSION     DAZPEU010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.425"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..85
                     /locus_tag="ACHU2L_004435"
     CDS             <1..85
                     /locus_tag="ACHU2L_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358980.1"
                     /translation="LIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            112..459
                     /locus_tag="ACHU2L_004436"
                     /pseudo
     CDS             112..459
                     /locus_tag="ACHU2L_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            456..551
                     /locus_tag="ACHU2L_004437"
                     /pseudo
     CDS             456..551
                     /locus_tag="ACHU2L_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            814..1062
                     /locus_tag="ACHU2L_004438"
     CDS             814..1062
                     /locus_tag="ACHU2L_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1358981.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          427 a          358 c          319 g          353 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca ctcccccgct
      121 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
      181 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
      241 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
      301 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
      361 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
      421 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
      481 ggtcacatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
      541 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
      601 ctttagaata atcaaagcgt tggattgcca tgtcatatga aaatcggcaa agttcatatg
      661 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
      721 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
      781 tactgtataa aaacacagtg cagagggatt tttatgcgtt ttgaaatctg catagccaaa
      841 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
      901 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
      961 ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg
     1021 aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt
     1081 aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg
     1141 ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg
     1201 cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca
     1261 gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg
     1321 ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat accagtggca
     1381 ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgcccccc
     1441 cccaaaaagt atcgttt
//
LOCUS       DAZPEU010000419         1434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPEU010000419 DAZPEU010000000
VERSION     DAZPEU010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.426"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            431..600
                     /locus_tag="ACHU2L_004439"
                     /pseudo
     CDS             431..600
                     /locus_tag="ACHU2L_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1434
                     /locus_tag="ACHU2L_004440"
     CDS             746..>1434
                     /locus_tag="ACHU2L_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1358982.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          331 c          445 g          311 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggt
//
LOCUS       DAZPEU010000420         1417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPEU010000420 DAZPEU010000000
VERSION     DAZPEU010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.427"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            38..442
                     /gene="stpA"
                     /locus_tag="ACHU2L_004441"
     CDS             38..442
                     /gene="stpA"
                     /locus_tag="ACHU2L_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR1358983.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(489..1013)
                     /gene="ygaP"
                     /locus_tag="ACHU2L_004442"
     CDS             complement(489..1013)
                     /gene="ygaP"
                     /locus_tag="ACHU2L_004442"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR1358984.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1023..1322)
                     /locus_tag="ACHU2L_004443"
     CDS             complement(1023..1322)
                     /locus_tag="ACHU2L_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1358985.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          382 a          332 c          335 g          368 t
ORIGIN      
        1 tacttttttg ttttggcgtt aaaaggtttt ctttattatg tccgtaatgt tacaaagttt
       61 aaataacatt cgcaccctcc gtgcgatggc tcgcgaattc tccattgacg ttcttgaaga
      121 aatgctcgaa aaattcaggg ttgtcactaa agaaagacgt gaagaagaag aacagcagca
      181 gcgtgaactg gctgagcgtc aggaaaaaat tagcacctgg ctggagctga tgaaagctga
      241 cggaattaac ccggaagagt tattgggtaa tagctctgct gctgcaccac gtgctggtaa
      301 aaaacgccag ccgcgtccgg cgaaatataa attcactgat gttaacggtg aaactaaaac
      361 ctggaccggt cagggccgta caccgaagcc aattgctcag gcgctggcag aaggtaaatc
      421 tctcgacgat ttcctgatct aattactgag gccggatgtc gctgctaggg cgcgtccggc
      481 gtcgcttctc aagctcgttg gttccacggc atcttatcta acaaccttgc cattccacaa
      541 aaaccgctga ttcctgcaaa cagtaacccg gcaccaacaa agccacttaa taagaagaaa
      601 ccgctattta cggtataacc cagtacaacg ccgtttaata ttaaaccacc cgcagcgatc
      661 tgcacctggc gcattaacgg caagggttga gatttattta ccgctactgg caatccagct
      721 cttttccagc catcaatccc atcttcgagt aaaaatattt ctgcgggggc ggcaatcgct
      781 gctaatttat cggcgttatt actggtgcgt ttacctgcct ggcagtggaa tataatttgc
      841 tcgcgacgta atttaggtgg aagacctgac tgttccagca cggataatgg agccagatct
      901 gcttcaggaa tatgttcacg aagatattca tcagcgtcac gaatatcgat taactttgcg
      961 ccgcgtgcga ttaattcttg tgcatcatgc ggcgaaatgg ttgtcaaagc catagtgact
     1021 ccttacggac aatagacatt tttcagggtg gcgataatgg catttaccgc ctcattttta
     1081 atggaatata gaatgcgttg ggcatcccgt tggctgttga taagcccttc gtcccgcata
     1141 cgagcgagat gctgtgatgt cgcagaggcg ctcagtccgg taatgcgcgt cagctctccc
     1201 gcgctggtgc cgggggaacc gctaagcatg cacagaatca gcaaccgttt agggtggctc
     1261 attgctttca ataaggccgc tgcctgttcg gtactggcct gtaattgcgc gagttcagtc
     1321 atatttattt aagtattttc taaattaagt aaactctaaa ctaaaaatgc aacatatacc
     1381 agcctcagca gcgtaaatga gagtaaaagc gtaagct
//
LOCUS       DAZPEU010000421         1412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPEU010000421 DAZPEU010000000
VERSION     DAZPEU010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.428"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..292
                     /locus_tag="ACHU2L_004444"
     CDS             <1..292
                     /locus_tag="ACHU2L_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1358986.1"
                     /translation="AYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFES
                     LCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            298..1395
                     /locus_tag="ACHU2L_004445"
     CDS             298..1395
                     /locus_tag="ACHU2L_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1358987.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          437 a          320 c          346 g          309 t
ORIGIN      
        1 cgcttatccg atttttgatc gtattgaaga aatggcatgg gctcgtcact accagcagat
       61 cgttcgtgaa gaaaaagaaa cggaactggc ggacgacctg gaaaaaggtc tgcctcagca
      121 cctgtttgaa tcgctctgca tcgaccattt gcaacgccac ggtgccaata aacaggcaat
      181 cagccacgca tttgatgatg atgttgagtt tcaggagcgc atggcagaac acatccggta
      241 catggctgaa accattgccc accaccaggt tgatattgat ttggaggact aaaatctatg
      301 gcgctttttc aacgagcaat aaacacacag gcctatctaa aagcaggaat tatggggttt
      361 gctggcgacg gcaaaaccta cacagcaagc gaactggcta ttggtctggt aatgcttatg
      421 cggcaacggg gaattgaagc tggtaaccgc ccggtaatgt ttctggacac agaaacaggc
      481 tctgactggg ttaaaccccg ctttgatgcg gaaaatatcg aattatttac ggcaaaaacc
      541 cgctcgtttg ttgatctgct tgaagccatt aatgaagcgg aaagcagcgg ttccgtaatg
      601 atcatcgact ccatcagtca cttctggacc ggattatgcg acgagtatgc cagacgccga
      661 aaccgtaaac gaggactgga attttcagac tgggcgtggc tcaaacagga gtggcgacgc
      721 tttaccgatc gatttgttaa cagtcaggcg cacattatta tgtgcgggcg tgcaggatat
      781 gagtatgact ttttcgaagg tgatgatggc aaacgccagt tggcaaaaac cggtataaaa
      841 atgaaagcag aaacagaaac cggttatgaa ccctccatcc ggatccagat ggagaagcaa
      901 atgaacatag aaaccggtca ggtgtggcgc accgccagaa tactaaaaga tcgctccacc
      961 cgtattgatg gacaggtatt ttcaaaccca acatttaaaa actttcttcc tcatatcgag
     1021 tctcttaacc tgggaggtga acaccccggc atcgatacct cacgggataa cagtgaatta
     1081 tttgccaacg acggtacacc gacatggcta aaagaaaagc gtgcaaaaga aatcgcactt
     1141 gatgaaatta tcgagttgct caacaagcac catgggggca ccagtaacga cgccaaacgc
     1201 gcaaaagctg atttgctgga aaaaacattc acctcccgct catgggaaag aatcaaaggt
     1261 atggactggc cgacaatcaa agctggtcgt aatgcactat ggattgaact ggaaggcgtt
     1321 gaatatgcat tcccggatcc acaaacacaa aacgaaacgc aacaagcagg atacgatgag
     1381 aatattccgg tgtaaaacca agtgcatttt gg
//
LOCUS       DAZPEU010000422         1364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPEU010000422 DAZPEU010000000
VERSION     DAZPEU010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.429"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            319..>1364
                     /locus_tag="ACHU2L_004446"
     CDS             319..>1364
                     /locus_tag="ACHU2L_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1358988.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          446 a          300 c          217 g          401 t
ORIGIN      
        1 actatgtata taagcttatt ttatacatga ttattagcaa caaatattac caagtcaagt
       61 agtcaggctg gctaaaatag ttggagatgt tgtagtatct tactcaagac taatgtcttc
      121 tggtataaat cctctgcatt gtgaatatgt aaaacatcaa aaaaagatga accaaatata
      181 cgaaaaaagc aaatatactt accagggtag aaagcatgat ataacactca ttcccttatg
      241 gcaaatattc tgaaacaccc tcctcattag gagagataca ttaaatacaa tttattcaca
      301 atctaattcc atatccatat gcgagaaatt aatatgctca gaaacatttc atcctgttta
      361 tttccacata tcagcacaat tacatccccc aaccattatt tgtccgaatg ggatgattgg
      421 gagaaacagg ggttaccgga agaacagcgt actgaggcgg taagaagact tcgtgcatgt
      481 cttacctcta aggggcataa actggacctg cgagccttgg cgctttcctc gctacctgta
      541 ctccctgctt gcattaaaaa gcttgatgtg agctgtaata aattaaccat ccttactgat
      601 ttacctgaaa atattaaaga acttattgca agagataatt tcttaacaca tatatctgca
      661 ttaccacatt atctaataac tttggatgtg tccgaaaatc aattagagaa tctgccgtta
      721 ttaccagaca ccatcaaatc actaagcgca gagtataata ggttatccac actgccttca
      781 ttacccttga atttaaaaaa acttgaggtt aggaacaacg aactgcaaac tcttccatct
      841 ctgccttcta atcttaagat acttaaggtt gcgcacaacc atcttactga actgccccct
      901 ttacctagga gactgcaact tctttttgca tatagcaata gattaagcaa cttaccaaac
      961 atccaagaaa atattatcat gagaagattt ttttattttg aaaacaacca aataactaca
     1021 atcccgacaa atctttttcg tttagatcct catataacta ttgagattgc aaataacccc
     1081 ttatcagatc aaactctgct attcttaata cagcaaactt cggttccaaa ttttaacggg
     1141 cctcagtttc gtatttccct gtcagaccaa aacagactgt ttttacgcca gatgttgccg
     1201 caaaatttac attcgcgcca tatcagagtc atcactgaag gggggcagaa ctttcagatc
     1261 ccccctcttc ccgaaactgt ggcagcctgg tttcctgaag cagatcgtcg ggaggtttct
     1321 acacaatgga cttctttttc caccgaggag aattcccggg catt
//
LOCUS       DAZPEU010000423         1346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPEU010000423 DAZPEU010000000
VERSION     DAZPEU010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.430"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHU2L_004447"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHU2L_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHU2L_004448"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHU2L_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR1358989.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          370 a          310 c          284 g          382 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatat
//
LOCUS       DAZPEU010000424         1342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPEU010000424 DAZPEU010000000
VERSION     DAZPEU010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.431"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..890
                     /locus_tag="ACHU2L_004449"
     CDS             <1..890
                     /locus_tag="ACHU2L_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358990.1"
                     /translation="HAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQKVFLGSEKP
                     LFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKTVAAPIMATGVGLALMYAT
                     SSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSPAFDKWNELVDKLNHERNT
                     VPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRLADS
                     GEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNKFNVGLQGNINTDVLQDSF
                     FKNVDFIHEYNSYTVGMGV"
BASE COUNT          401 a          252 c          316 g          373 t
ORIGIN      
        1 tccatgcagc aaggaaggtt cttagcggga taataggaaa tgtgccaaaa aataataaac
       61 caaaaacaat tatttttgat ttgcgttttc ttagtgcaat taagcagaaa gtttttttgg
      121 gcagtgaaaa accgttgttt gtaaagcata ccgcaatgat gagggaggca tgtaaggaat
      181 taccgcagtc agtggaatat gttcctttag cctgtgaagc gcatagccat cgcagcgtcg
      241 cccttgccaa aaccgtggct gcgcccatca tggcgacagg tgtgggcttg gcattaatgt
      301 atgcaacatc atcaggaccg tattatgcgc aaagtctttc tggttcgccg gatatcgaga
      361 tgataaaaaa tcgcatggct gaattgttta accagtcgga tatttctgtg cgcaacaaat
      421 atagcccggc ttttgataaa tggaatgaac tggtagataa attaaatcac gaaagaaata
      481 cagttccgtt tgcgtgtttg accaccattt tagcgacggc gataaatgaa actcttgagg
      541 gggatacgaa tgttgccgtt gtgatggggt gtaaatctgc gaaagatcgc acgatatcca
      601 ttgtccttgg caactcgatg ttgcaaacac tccttgaaaa acgcctcgcc gatagtggag
      661 aaatagaaaa tctttttgat caacagggat attttagttg cgattcatta acacccgaag
      721 aattaatgat gttaaaagac ctttttgata ttcgagttct tcatgtctct aacaaattca
      781 atgttggatt gcaaggtaat atcaacactg atgttttaca agattcgttt tttaaaaatg
      841 tcgatttcat tcatgaatat aactcgtata ccgtagggat gggagtctga cgagtaaaac
      901 tcattaggat tatcagaatt cggacaatgc attgcaaaaa tgacatacaa agagagatgt
      961 gtgcaggtac ttagccgcga ggggcatggc gaagccatac agaaagtacc agtattcaga
     1021 actccagtga aagcggcata gtggccgcct gctcaggagt tttgctatga cccgtaatcg
     1081 tgctgaacgt cgccacccta tgatgcgatt aaaaaaactc atcgtcgcgc tgatgattga
     1141 cgtcaatgct atcggatgat gccgggggtg ttccggaagg aattccttgt ttcccgaact
     1201 gttaccaaca aaattataac gatgagcaag taaagaccgg ttggtggcag tactgaccgg
     1261 tctgtgatga aagattaatt tcaccttaaa tcctggtgct gttaggatgc tgacgttttt
     1321 cggctacacc attcagatta tg
//
LOCUS       DAZPEU010000425         1330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPEU010000425 DAZPEU010000000
VERSION     DAZPEU010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.432"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(41..100)
                     /locus_tag="ACHU2L_004450"
     CDS             complement(41..100)
                     /locus_tag="ACHU2L_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1358991.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACHU2L_004451"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACHU2L_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAZPEU010000426         1326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPEU010000426 DAZPEU010000000
VERSION     DAZPEU010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.433"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHU2L_004452"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHU2L_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1358992.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="ACHU2L_004453"
     CDS             complement(590..1255)
                     /locus_tag="ACHU2L_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR1358993.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          330 a          339 c          311 g          346 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgt
//
LOCUS       DAZPEU010000427         1293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPEU010000427 DAZPEU010000000
VERSION     DAZPEU010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.434"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            111..1293
                     /locus_tag="ACHU2L_004454"
                     /pseudo
     CDS             111..1293
                     /locus_tag="ACHU2L_004454"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          364 a          233 c          331 g          365 t
ORIGIN      
        1 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
       61 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
      121 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
      181 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
      241 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
      301 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
      361 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
      421 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
      481 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
      541 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
      601 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
      661 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
      721 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
      781 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
      841 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
      901 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
      961 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     1021 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     1081 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     1141 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     1201 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     1261 taaatatcgt attcgttata gtaaatccat tag
//
LOCUS       DAZPEU010000428         1284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPEU010000428 DAZPEU010000000
VERSION     DAZPEU010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.435"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            522..806
                     /locus_tag="ACHU2L_004455"
     CDS             522..806
                     /locus_tag="ACHU2L_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR1358994.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            806..1081
                     /locus_tag="ACHU2L_004456"
     CDS             806..1081
                     /locus_tag="ACHU2L_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1358995.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          339 a          321 c          324 g          300 t
ORIGIN      
        1 acagaataag tttacgcgta aatcgccctg aatccagaag agcattaagt atcacataaa
       61 caggttccat accagtcctg accgcatcag aacgccagta aataaaatct cgtagtccac
      121 cggcatcaga gacaagagta atcgtcgtta ccggtatttc gactggtttg tactggtgtg
      181 agcggacaaa actactgtac gtctctcata cacgctcgta agcggccctc acggcccgct
      241 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac
      301 agaggcattt tcatcgctga caggccgcat ggcctcgcag agccggaggg ggactggtga
      361 tattcggacg accgtaccgg cttgcgccgg gcttcggcgg ttttacacct gtgctacatg
      421 taaccaccgt atgccgcctt atatgcgctg actcgcatta tttgccttgt tccgtctgaa
      481 agtaatcact atgattaaat atgattaaca actaatcggg tatgcaaatg aaaaacaata
      541 ccgcacaagc aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg
      601 accagatggc cgccagactg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
      661 catggcttgc ccaagaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
      721 catccggaca ggttatcgac catcaggctg tacagtcctg ggcggacagc ctcagtactg
      781 accatctgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cactttctga
      841 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaacggt
      901 gcagtccctg acacaggcgc cggtcattct gttaactcat ccacgtatgg gagaacagtt
      961 gtttcagttt gaacccaggg aggtcagacg gatttttact ggcgagtacg aaatccgtta
     1021 cgaacttact ggccagacta tttatgtatt gcgcctgtgg cacacacgag agaacaggta
     1081 gattgggtgt ggaggcaact ttaacatagc ctgcaggtat taattctgga gcattgttat
     1141 caggccagat attttgcatt ccattaaata atcttgtagg agataaaata aattacgatg
     1201 aaataacgaa aattaccgct cgtgactggc ggcaatatcg cgctccgggc tggcaaataa
     1261 cccatcagaa acgttattgc caga
//
LOCUS       DAZPEU010000429         1277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPEU010000429 DAZPEU010000000
VERSION     DAZPEU010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.436"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACHU2L_004457"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACHU2L_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR1358996.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          359 a          229 c          285 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actatctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAZPEU010000430         1263 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPEU010000430 DAZPEU010000000
VERSION     DAZPEU010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.437"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..1095
                     /locus_tag="ACHU2L_004458"
     CDS             <1..1095
                     /locus_tag="ACHU2L_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1358997.1"
                     /translation="QSLAYYLRVLVREGLWIHGLYGYAAQYLYDFTSFDTTPYAKKLL
                     EMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKNGCDDAYLKKIISYFG
                     QLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGLFGELPSGYVRGALSL
                     LRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPVYKEGEIQYDANNFTL
                     MRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILIDKEYVYDGKTLAEVI
                     MENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKSPVDEIKNVMMLYGDK
                     AENRNRRRMIYDALTIICEDDIRNNPPELS"
BASE COUNT          406 a          171 c          281 g          405 t
ORIGIN      
        1 caaagccttg catattatct cagagtgttg gtgagagaag gactctggat tcatggtttg
       61 tatggttacg ctgcgcaata tctttatgac tttaccagct ttgatacaac gccttatgca
      121 aaaaaattac tggaaatgtt ttataagttt caaaacagtg cagaaggaac attgtctcgt
      181 tatagtgttt cacttgatga taaatctcag gaatatattt cccgtctgaa agatcttgta
      241 tttaatatta atgataagaa tggttgtgat gacgcatatc tgaaaaagat tataagctat
      301 tttgggcagt tgcagaatga ggctgttcat gtgaccagct gctacgaaac acttgcggtg
      361 tacatttgtc tgataaggaa aaataaaatt aatgatgtgc tgcagcatta tgatgacatg
      421 gaaagaaaag gattgtttgg tgaattacct tcaggatatg tcaggggggc attatcgttg
      481 cttcggacag cactggaggt taaagtaaat agaaaaaata taaagtatgg ctcgttgttt
      541 tattggcttg accatgtaaa agcctatcag gatgcattta ttgaaaaaat accacttatt
      601 gatcctgtat ataaggaggg tgaaatacag tatgatgcga acaattttac cctgatgcgt
      661 gttattaaga tgtataattg tatgcttgaa aagatcagta cgaaaccata tattgcgccc
      721 ccatatatta caggtttgct tgatgacgta gagaaggtgc tggataagat aaatattctg
      781 atagataaag agtatgttta tgatggtaag acgctggcag aagtcattat ggaaaacaaa
      841 gtattatcca gtcgcgaacg taaagaaact atgatagggc ttttcacagg cagtaagaaa
      901 tatactttac tgcaatgtgt tgagaagctt ggtgtattag ttcactatgt gaaatcaccg
      961 gtcgatgaaa ttaaaaatgt gatgatgttg tatggcgata aggccgaaaa caggaacaga
     1021 cgcagaatga tatatgatgc gttaaccatt atttgtgaag atgacatcag gaataaccct
     1081 cctgagctgt cgtgaattca tctgagtgtg ttttgggtca ataatcttta tataccctgc
     1141 taattttgct aaccattagc aaaattagca ggggtattat ctatgtctgg caagagaaaa
     1201 ttctataata gtatgattta tttgtttgtt ttgtttttca attagaatgg tgtgtttttg
     1261 tga
//
LOCUS       DAZPEU010000431         1261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPEU010000431 DAZPEU010000000
VERSION     DAZPEU010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.438"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(51..995)
                     /gene="leuO"
                     /locus_tag="ACHU2L_004459"
     CDS             complement(51..995)
                     /gene="leuO"
                     /locus_tag="ACHU2L_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR1358998.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          384 a          285 c          253 g          339 t
ORIGIN      
        1 aaaaatgcag aataaaccag acattcatgt ctggcctatt ctgcattcag ttagcgtttg
       61 caaattgaga ctaattgttc ttccatccac tgatggcctt tatcgcgccc ggcagcttca
      121 tgccaagaga gataacaggt tctgctgttt tgttttaacg gcagcggtaa tacctgtaat
      181 tctaaggatt cagcgaactc ttcagccagc caacgcggcg caatagcgac caaatgcgtt
      241 tgcgacacca cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc
      301 ttatctaccg tgtcatacca aggttgacta aatgacgcga aacgatcgag cgaaaccgcc
      361 gcatgttgtt cgttataaac atcatgtttc agtaacgggc ctttaattgt tggatgattt
      421 ttgctggcta ccagcaccat ttcatcttta aataatggca cgctggtaaa ttcaggacga
      481 tggaagtctt cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg
      541 ttctgattta atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga
      601 ttataaatct gcgaggtcag aatgctgtct aacgggctgc aaacacaaag atgaaataca
      661 cgttcactgc tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat
      721 gcctggcgaa ctgaaccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca
      781 taacgaacaa aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc
      841 gcaggttgcg acatacccag aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg
      901 gcatcgaaaa cggttaataa gttgagatcg accatgcgta gctgtggctt gcttaactcc
      961 gccgtctctg aatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac
     1021 tccctttccc ttattggaat gcagagaaat aatcctgaag aatatgattt atcatgcata
     1081 taaaataaga aaaagcaaaa tgagtaaaat tcggaaaaca taaagatacg ggttctgata
     1141 aaaacagaat ccattgccgt ggaatgagtc atttaccaca gcataataat ccataatgtt
     1201 ttgttgcgaa aacaatctaa tcataactac cgcgaatact caatcatcta caaaatggat
     1261 t
//
LOCUS       DAZPEU010000432         1255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPEU010000432 DAZPEU010000000
VERSION     DAZPEU010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.439"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            121..>1255
                     /gene="pdeF"
                     /locus_tag="ACHU2L_004460"
     CDS             121..>1255
                     /gene="pdeF"
                     /locus_tag="ACHU2L_004460"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1358999.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          282 a          280 c          281 g          412 t
ORIGIN      
        1 tttcatatga cacttagaat caatgctctc tatagtgatg aataatcatc attcgaagtc
       61 aggtgggatg cctgtctgaa tacacctcct tcaggatgtg ggggattctg ctgagcatct
      121 atgaaactga atgcaactta tataaaaata cgtgataaat ggtgggggct tccgctgttc
      181 ctgccttctt taatcttgcc cattttcgcc cacattaata ctttcgcgca tatttcttcc
      241 ggtgaggttt ttctctttta tctgcctttg gcactgatga tcagcatgat gatgtttttc
      301 agctgggcgg cattgccagg gatcacctta gggatttttg tccgcaaata tgcagagctg
      361 ggtttttacg aaacgctctc attaacggct aattttatta tcattatcat tctctgttgg
      421 ggcggttaca gggtctttac tccccggcgt aacaacgttt cacatggtga tacccgttta
      481 atttcccagc gtatattctg gcagatagtg tttcctgcaa cgctgtttct gatacttttc
      541 cagtttgctg catttgtagg attactggcg agcagagaaa atctggtcgg tgtcatgcct
      601 tttaacctcg ggactttaat caattatcag gccttactgg tgggtaatct gatcggtgtc
      661 ccgctgtgct acttcatcat tcgggtagtg cgaaatccat tttatttacg tagctattat
      721 tcgcaattaa aacagcaggt tgatgccaaa gtcaccaaaa aagagttcgc gctctggcta
      781 ctggcattag gtgctttatt gttgctgtta tgcatgccgt taaatgaaaa aagcacaatt
      841 tttagcacca attatacctt gtcattattg ctgcccctga tgatgtgggg agcaatgcgc
      901 tatggttata agctgatttc gctgctctgg gcggtcgtgt tgatgatcag catccacagc
      961 tatcaaaatt acattcccat ttatcctggc tataccacgc agctgaccat aacctcctcc
     1021 agttatctgg tattctcttt tattgtcaat tatatggctg tactggcaac tcgtcagcga
     1081 gcggtagtca gacgcattca gcggcttgcg tatgtggacc cggtggttca tctgccaaat
     1141 gttcgcgccc tgaatcgcgc gttgcgtgat gccccctggt ctgcgctttg ttatttacgc
     1201 atccctggca tggaaatgct ggttaagaac tatggcatca tgctgcggat tcaat
//
LOCUS       DAZPEU010000433         1244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPEU010000433 DAZPEU010000000
VERSION     DAZPEU010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.440"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..846
                     /gene="ldtC"
                     /locus_tag="ACHU2L_004461"
     CDS             <1..846
                     /gene="ldtC"
                     /locus_tag="ACHU2L_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR1359000.1"
                     /translation="LVGENKFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPR
                     AGSVLTIPLQTLLPDAPREGIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPT
                     MVTTVSDKRANPTWTPTANIRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYL
                     LHGTNADFGIGMRVSSGCIRLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLV
                     EVHQPLSEKIDDDPQLLPITLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQV
                     SPQTL"
     gene            complement(929..1141)
                     /locus_tag="ACHU2L_004462"
     CDS             complement(929..1141)
                     /locus_tag="ACHU2L_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR1359001.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT          322 a          310 c          313 g          299 t
ORIGIN      
        1 ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct ggaagccatc
       61 gccaaaaaat ataacgtcgg ctttctcgct ctgttacaag ctaaccccgg cgttgatcct
      121 tacgtaccgc gcgcgggcag cgtgttaacg atcccgttgc aaaccctact tccagacgcg
      181 ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc tctattacta cccgccgggt
      241 aaaaattcgg taaccgtgta tccaataggt attggtcagt taggtggtga cacgctgaca
      301 ccgacaatgg tgaccaccgt ttcagacaaa cgtgcaaacc caacctggac gccaacggca
      361 aacatccgcg cccgttataa agcacaggga attgagttgc ctgcggtagt gccggctgga
      421 ccggataacc caatgggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg
      481 cttcacggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt
      541 ctgcgggatg acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat
      601 atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta
      661 catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat
      721 agcgcaatgc aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg
      781 atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact
      841 ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga aaaaatcagc
      901 agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg
      961 tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt
     1021 gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg atagtaccga
     1081 ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagttg gcaaatgaca
     1141 tggagcttaa aatcgccgca gcgatgaggt ttttacgttt ttcataatag tggccttatg
     1201 cagatgaatg acgtcgcaag aaattactta acgatcgata acta
//
LOCUS       DAZPEU010000434         1238 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPEU010000434 DAZPEU010000000
VERSION     DAZPEU010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.441"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..128
                     /locus_tag="ACHU2L_004463"
     CDS             <1..128
                     /locus_tag="ACHU2L_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359002.1"
                     /translation="DLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(167..970)
                     /gene="dkgB"
                     /locus_tag="ACHU2L_004464"
     CDS             complement(167..970)
                     /gene="dkgB"
                     /locus_tag="ACHU2L_004464"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR1359003.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(1133..1209)
                     /locus_tag="ACHU2L_004465"
     tRNA            complement(1133..1209)
                     /locus_tag="ACHU2L_004465"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Asp,seq:gtc)
BASE COUNT          328 a          291 c          290 g          329 t
ORIGIN      
        1 gcgatcttgc aagtatggga caacttgtga aattaccgac gagaagggga agggccttcc
       61 cgagagtggt aaaggagagg ccctggaaat accccacagc cccgaaaaag agccagtcag
      121 ttgcttaact gactggcatt actcccggag gagctgtcag agaggcttaa tcccattcag
      181 gagccagacc ttccgggcta accaggcggt cgttgcaatc cagtgcggcg atcgcttttt
      241 tatcttcggc atcaagctgt aaattttgtg ccttaagatt actttccagg tttttacgtt
      301 tagtagaaga aggaattact gagtaacctt cccccatagc ccacgccaga atcacttgtg
      361 ccggaatcgc attgtgttta gctgcaatac gagcaataac ctcatctttc agtgccttac
      421 cataagccag cgtcatatag gaagtgatat gaataccgtg ctgtttagcc caggcaacca
      481 ctttacggtt ttgcagataa ggagagagtt caatctggtt ggtagcgatg ttttcagcgc
      541 caacagcagc aatcactttt tccatcaatg ggatcgtgaa gttggaaata ccgatctcac
      601 gcgtcagccc ttgttttttg gcttccagca gcgcctgcat aaactcttca acagagactt
      661 catcgtttgg tgacggccag tggattagcg tcagatcaac ataatcggta cgcaattttt
      721 gcaggctctc tttcagactc gggatcaatt tgtctttgct gagattttca atccagattt
      781 tagtggtgat gtagagttca tgacgtggca cgccactttc tgcaatcgcc tgacctactg
      841 cggcttcgtt atcatagatt tgtgcggtat caattgcgcg ataaccaagt tcaagcgccg
      901 ttttcacaga tgaaataaca acgtcgtctt tcagacggaa agtacctaaa ccaaatgcag
      961 ggatagccat aatatgcctc ttttaatgct attttcgtta actgaggtga ttatgggctt
     1021 cagactgttg cgggaaaaga gcgtaaaacg cagaggattt ttgcgattct agcaataata
     1081 gatatccaga cgcaaaaaaa catcgagcgt taactcgagg cttcttaata agtggcggaa
     1141 cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac
     1201 taccgctcca ccgaattctt ttacaaccac cggtttta
//
LOCUS       DAZPEU010000435         1237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPEU010000435 DAZPEU010000000
VERSION     DAZPEU010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.442"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(271..>1237)
                     /locus_tag="ACHU2L_004466"
     CDS             complement(271..>1237)
                     /locus_tag="ACHU2L_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1359004.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          369 a          273 c          265 g          330 t
ORIGIN      
        1 tatttaaaaa tgtgttgtat tagcgactga aatctgcctt ttaaaaagga ggagggtact
       61 gagacaaaga gtcattgctt ctccgataaa atgtcagaga agcatgatta cgtggttatt
      121 gtctgttgat gacttgttga ccgagccgtc atcattctca atgcattagt taaatcattt
      181 gtgattttta aggattatcc tgtctatgat tttctactta gaaggatatg gcactaacta
      241 tgcatcttat agtttactgc tggcatctat ttaaaatagc aattgagtca aatagcccat
      301 caagatagcc atacctaata taaccgtcag gaatgcagct atcatcggca ttctgaacat
      361 tgatttaagt aatatcactt ccgtcaggct tgcgccggca ctgccgatga ttaatgccat
      421 taatgctccc agacccattc cttttgtcat cagaacagat gccagaggga taaccgcctc
      481 agcccggata tacagcggaa taccgacaac ggcgcttaat gggatggcga agggattatc
      541 tgctcctgca tgagcggcaa tccacgctga gggaataaag ccataaataa aagatcctat
      601 taatactcct aacaaaagat atggcagtac atctttaaac tgttgcaacg catctttggt
      661 ggctagtttt atgggattaa tacttactac agctttggta ctgcaacagg taactacggg
      721 gttctcaatg gctttagccg ctggactaca acaactcact tttatgggcg tatatgtcgt
      781 tcccggcgaa gtttttgctg gagcacaaca atttgctgat gagcttttac tggcaatgat
      841 atgacgttca aaccccagag aatccaggat aatactggca agaacggaga cgccggctgc
      901 gataatcgcg tacaacaagg tgactttcca accaaaggtc atccacatta gtccgacgat
      961 aatgggatta agtaatggtg aaacaaataa aaaagtgaga gtcggaccaa acccggcttt
     1021 cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg gggtaacggc
     1081 tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca tctgctggat
     1141 tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca ctataaacag
     1201 caacgaaagc tcgacggcga gaaacacaaa catctct
//
LOCUS       DAZPEU010000436         1220 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPEU010000436 DAZPEU010000000
VERSION     DAZPEU010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.443"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            181..1179
                     /locus_tag="ACHU2L_004467"
                     /pseudo
     CDS             181..1179
                     /locus_tag="ACHU2L_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT          280 a          272 c          274 g          394 t
ORIGIN      
        1 ccgctaaaaa atggcactcg gcacagtaaa ccccgctata gttatgctat gcatttgtct
       61 ttaatgatat ctcgtcgtta tattaacagg ctgaaattat cattaatgca catttcaata
      121 ttagcaggga tacctgagga gtttaaagtg aacatatata tcgggtagct tttcaaatta
      181 atccctttga ttataggctt aatttgcatc gcgttaggtg gctttgtgct ggaaagttca
      241 gggcaacgtc acgggtcatg tgctgatttc tctggcggcc atatgcctgg cattattcac
      301 taccgcattt attatcattt cgcaactcac gcgcggcgtt aatacgtttt acaatacatt
      361 gttccccatt attggctatg cggggtcaat tatcaccatg atatggggtt gggcactgtt
      421 agcaggtaat gatgtgatgg cagacgagtt tgttgccggc catgttattt ttggcgttgg
      481 tatgattgcc gcatgtgtat cgacggtggc agcgtcgtcc ggtcactttc tgctcattcc
      541 caaaaatgca gcggggagca agagcgacgg aacaccggta caggcttatt cttcattaat
      601 cggtaattgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca tttggtctat
      661 tacgctgtta catagtgccg acataactcc gcattatgtc gccttgggtt aaccgcaatc
      721 tgtgcctgtc taattggcct agtggccaca atcgtccatc aaacacgtaa tacgttctca
      781 tccaaagagc actggctgtg gtgttattgg gttatttttc tcggctcagt cacggtgctg
      841 caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc cggcattatt
      901 ctcatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct actggcactg
      961 gtctggagac gtacctgttc gttagccaac agaataccga tgataccggt atttacctgt
     1021 ctgttttgct tgtttttgtc atcattcctc gcggaaatcg cacaaactga gctggactac
     1081 tttattccat ctcgggttct ggttggatta ggcgcagtat gttttacatt gttctctatt
     1141 gtttcaatat tagaggccgg ttctgcgaaa aaaaattaaa atttacataa tcccagcgtt
     1201 attcatatct taacgctggt
//
LOCUS       DAZPEU010000437         1188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPEU010000437 DAZPEU010000000
VERSION     DAZPEU010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.444"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>1188
                     /locus_tag="ACHU2L_004468"
     CDS             <1..>1188
                     /locus_tag="ACHU2L_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359005.1"
                     /translation="SIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQP
                     INSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETISS
                     TPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSVQ
                     TVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNMT
                     GQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQINGL
                     FSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFTT
                     PSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPSG
                     KRI"
BASE COUNT          461 a          226 c          176 g          325 t
ORIGIN      
        1 tgtcaattga ctttacccca ggtataataa atacatatca cggcgatatt tataactgca
       61 caacaaatac cgataatgtt aaaacgccag acacaccgaa gtggccttgt gataattggg
      121 aagaacaaca acccattaat tccacattct ctggagaagg atacaaccct gaacaatttg
      181 atttagcgca acaacaactc caacaaatta atgcatgcca cacgaacacc acatatacaa
      241 atgcagacta ctcaaaagtt gtggttcaac ttgtaagtct tattaataac attgaaacga
      301 taagttcgac accacttacg caacagacac aatcaatact taatcagatt cataacatta
      361 gatacgaaaa ggataaggat tctgtatgtc aaatcattgt tgtcgttaat ccagaaccca
      421 acaatccaat cataactaaa attctcgtag aagaaggaat acccgaaaga ttcagtgtcc
      481 aaaccgtttc ttccgataat aaaaattttg ctgggcaacg agctgactta ccaactgata
      541 taagagacat tcaaagcttg tacttaaaaa tggcgaagct gtatattgag cacagtaaaa
      601 atgaaaatag gatggaagcc cttgctggat gcgactttat tgatttcaac atgacagggc
      661 aagatatgtc caagctagtc ctaaccttat caaaatttta ttttgaagat ctacttaata
      721 taaatttcac tgacgcaaat ttatttaaca ctattttttc acacaaggaa aatcccatcc
      781 ccaaactaca taaatatgaa caacatcttg acaaacaaat caatggtttg ttttctacat
      841 tgttaactat taatgacaac tctttacgcg cgaaagcgga aattgcttcg agaataattg
      901 atttcttaga ggcaaaggta gtgaatcttt catttgatga catattaaaa tataagcaag
      961 agttcaaaaa aatatgttat aaacaacttc aagagtttac aacaccctct ctatacaata
     1021 aaattcaaaa atgggcaaca atgtctaaaa atgaattcat agaatttcat tacgagacac
     1081 ttcaacccga aaagatttca tacccctttt atttaaagag agatctgcct aatgaaaaag
     1141 atataaatta tggggtggaa atagaaatac catctggcaa aagaattc
//
LOCUS       DAZPEU010000438         1185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPEU010000438 DAZPEU010000000
VERSION     DAZPEU010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.445"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..1103)
                     /locus_tag="ACHU2L_004469"
     CDS             complement(<1..1103)
                     /locus_tag="ACHU2L_004469"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1359006.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT          287 a          309 c          307 g          282 t
ORIGIN      
        1 gtacagatga actcatacgg ccccagtttg gcgatctcat ccgccagata ggcagcgacc
       61 tggtaggaag cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag
      121 tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatctga
      181 ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag
      361 ccaccgctgg cggcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagtgcat cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt attttcgtcg caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgcac cggaccgcac accaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatgtt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt gttaatggat aaatccatca acttgtggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggccaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctgaaatgc gacatcgtcg cgcatttcgt gcagcggaaa acgttttgat
     1021 tctgcgatag tggaaataga cttcgcacca aaacgtgaat cgagtagttc cgaccttaaa
     1081 tccgttactt gcttcttatc cattttaaac tccttaaaat gattggatcg cattaaaaaa
     1141 gtaggattta tcgataaagt aagcaagtgc aaaggactcg tgttt
//
LOCUS       DAZPEU010000439         1180 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPEU010000439 DAZPEU010000000
VERSION     DAZPEU010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.446"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHU2L_004470"
     CDS             complement(<1..330)
                     /locus_tag="ACHU2L_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFR1359007.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFG"
     gene            complement(461..934)
                     /gene="dfrA17"
                     /locus_tag="ACHU2L_004471"
     CDS             complement(461..934)
                     /gene="dfrA17"
                     /locus_tag="ACHU2L_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR1359008.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
BASE COUNT          318 a          289 c          263 g          310 t
ORIGIN      
        1 accgaactga agctcacgcc gcgccggata gcgccaaggc actacttcgc ttcgagcgac
       61 aacagttagc tccagcggtc gccatgttcc gccatcgcct ggcggtgatg agactttcag
      121 caaatcgagc attagcgcct gccggagcga atcgttaggt gcggcgctga cggtcacgag
      181 caagtctatg tcgctgtccg gcttcagccc tccatcgatc gcagatccga acaggtggat
      241 tgtgtccagt gtcgcagcca gatggcgctc gatcaccgcg cgagcgtggg acagctgctt
      301 gaaaacttgt gcagggaaaa attcacccat gatgcctaac gttaagttca gcggcagctt
      361 ttaagttgcg gctttgtgga atacttttgc gcagcaaaac cacaaagacg cgacttaaaa
      421 gctgtccaag gagcgaagcg actggtgctg caacgcattg ttagcctttt ttccaaatct
      481 ggtatgtata atttatatta gacataaaaa actgttcaaa aaccaaattg aaattctcag
      541 gcattatagg gaatttgata tcaccttcga cttcaacgtg aacagtagac aaatgaatta
      601 tatctgcttt ttcaataagg ctattataga tttgaccccc gccagagaca tatacatgat
      661 ctgtaacttt tgatagctct ttcaaagcat tttctattga aggaaaaact aggacgtttt
      721 catttgagct tgaaattccg ttctttgaca ctactgcata tttgcgattt ggaagaacac
      781 ccatagagtc aaatgttttt cttccgacaa ggagccattg attatatgtg agcgctttaa
      841 agagtagttg ctcacctttt actgaccacg ggatatcagg accactaccg attacgccat
      901 tttctgacac tgcagaaatc aatgatattt tcaatttaac tcccttaatg gctaactttg
      961 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     1021 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt
     1081 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     1141 tggaccagtt gcgtgagcgc atacgctact tgcattacag
//
LOCUS       DAZPEU010000440         1160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPEU010000440 DAZPEU010000000
VERSION     DAZPEU010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.447"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            115..>1160
                     /gene="cadB"
                     /locus_tag="ACHU2L_004472"
     CDS             115..>1160
                     /gene="cadB"
                     /locus_tag="ACHU2L_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR1359009.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          247 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga
//
LOCUS       DAZPEU010000441         1150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPEU010000441 DAZPEU010000000
VERSION     DAZPEU010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.448"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(385..522)
                     /locus_tag="ACHU2L_004473"
                     /pseudo
     CDS             complement(385..522)
                     /locus_tag="ACHU2L_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(604..902)
                     /locus_tag="ACHU2L_004474"
                     /pseudo
     CDS             complement(604..902)
                     /locus_tag="ACHU2L_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          340 a          238 c          198 g          374 t
ORIGIN      
        1 tatttattga catttttgtt catccttttt gctaaaaaaa aacaataacc tatttttgta
       61 atgtaataga tattagtcta ttgctaatct aaaaaaacca ttgccacatt gaatggctat
      121 attcaccaat aaattctttc gatttcgtat attgacatat cgtagatata tcttatccct
      181 aagattcaat aatcgagtcc gtttaccatt ctgtgcaagc gccttcactc agaagtgacc
      241 gtcaaggcga tcaccaaact tgataataaa gcagctcatt gccatacgcc agtccttaag
      301 gggcgttgtc catttctgtg atgctgccta gatttaactg ccagctccgg cttatctaca
      361 tctaaatatg tgagcctaat accgctacac tttgtccaga ctatgttttt ctccgggaag
      421 tgggctaacc gtctccataa agtgacgaac ttttttcaac agtggccata ttgaacggca
      481 ctgatgatta cgggttattg tctcatgaag agccggccac atcactgaca gcagtgactt
      541 caggccttca gcaccagaca gcctgaacca gttaatccat cgcccgacaa atttagtaat
      601 ctagggctgg tttttcttca gctgttgcgg ttcgaaagtg ccattacgat cacgcagagt
      661 acggatttcc agaggttcat ctccagtagt gataatcttc gttaagcaac cattgcggga
      721 attgctgcca gatctaagct gatttttctc gcacccaaga tgatgagtca tattagcatt
      781 gagtacggtt tcattttcag catccaatca aactgactga gatcttcagg atttttgaga
      841 cttttggcta gttcattagc taaagcccgc aacttattag atgtcaactg tttttcattc
      901 ataaattcac cttcatttga tatcaaatta aatagttcaa catcagagaa ttatacaaac
      961 ttatgtacag gctcctatga tcttcgaatc ctgaagatta tagtgaaaat aagttttcac
     1021 gttgaaatcc cacatagtta gtatatgata aataatcatg gatgacctga ttaacctctt
     1081 ggggtagtgc ttttatagaa gaataaatat tctactaatt taatacatca cctaaaatag
     1141 tattgttttt
//
LOCUS       DAZPEU010000442         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPEU010000442 DAZPEU010000000
VERSION     DAZPEU010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.449"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..730
                     /locus_tag="ACHU2L_004475"
     CDS             <1..730
                     /locus_tag="ACHU2L_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR1359010.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACHU2L_004476"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACHU2L_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAZPEU010000443         1137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPEU010000443 DAZPEU010000000
VERSION     DAZPEU010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.450"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..182
                     /locus_tag="ACHU2L_004477"
     CDS             <1..182
                     /locus_tag="ACHU2L_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFR1359011.1"
                     /translation="KLSEETKFIEDLSADSLSLIEMLDMISFEFNLRIDESTLEHIIT
                     IGDLISVVKNSTKSI"
     gene            399..>1137
                     /gene="virB"
                     /locus_tag="ACHU2L_004478"
     CDS             399..>1137
                     /gene="virB"
                     /locus_tag="ACHU2L_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR1359012.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKN"
BASE COUNT          431 a          198 c          178 g          330 t
ORIGIN      
        1 gcaaactatc tgaagaaact aaatttattg aagatctttc tgccgattct ctttctctga
       61 ttgaaatgct ggatatgatt agttttgagt tcaatctacg tatagatgaa tctacattag
      121 aacacattat tactattgga gatcttattt ctgtagtcaa aaatagtaca aaatcaatat
      181 agtaattgat aagcattttt tcatctatgg agctctcaca tcagagctcc acaagaatat
      241 tattctttta tccaataaag ataaattgca tcaatccagc tattaaaata gtaattttta
      301 agactaccgt tgactatcat caacagttac atacaatctt gctcacactg catttaactt
      361 ttgtcaatat aaatcaatat gaataaacag ggtgtgatat ggtggatttg tgcaacgact
      421 tgttaagtat aaaggaaggc caaaagaaag agtttacact ccattctggt aataaagttt
      481 cctttatcaa agccaagatt cctcataaaa ggatccaaga tttaaccttc gtcaaccaaa
      541 aaacgaatgt acgcgatcaa gaatccctaa cagaagaatc attagccgat atcataaaaa
      601 ctataaagct acaacaattc ttccctgtaa taggaaggga gattgatggt agaattgaaa
      661 ttctggatgg cactcgtaga agagcatctg caatatatgc aggagcagat cttgaagttc
      721 tatattcaaa agaatatata tctactcttg atgccagaaa actagcaaac gatatacaaa
      781 cagcaaaaga gcatagcatc cgagaacttg gtattggtct taattttctg aaagtatcag
      841 ggatgtccta taaagacata gccaaaaaag agaatctgtc tcgcgcgaaa gtcactcgtg
      901 cctttcaggc agcaagcgtt ccacaggaaa taatatctct atttccaatc gcgtcagaac
      961 ttaactttaa tgactacaag atattattca attattataa aggacttgaa aaggctaatg
     1021 aatctcttag ttctacacta ccaatattaa aggaagaaat aaaagacctt gatacaaatt
     1081 tgcccccgga catatataaa aaagaaattt taaacatcat aaagaaaagc aaaaaca
//
LOCUS       DAZPEU010000444         1133 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPEU010000444 DAZPEU010000000
VERSION     DAZPEU010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.451"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..348)
                     /gene="mxiC"
                     /locus_tag="ACHU2L_004479"
     CDS             complement(<1..348)
                     /gene="mxiC"
                     /locus_tag="ACHU2L_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR1359013.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFK"
     gene            complement(367..>1133)
                     /gene="mxiD"
                     /locus_tag="ACHU2L_004480"
     CDS             complement(367..>1133)
                     /gene="mxiD"
                     /locus_tag="ACHU2L_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR1359014.1"
                     /translation="SELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFIASV
                     MALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINVIP
                     RFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSLLIG
                     GYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISNIVRVFLIQPREIKESSYYNTAE
                     YKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY"
BASE COUNT          345 a          228 c          166 g          394 t
ORIGIN      
        1 tttaaaatat ctctttagtc tgtctataaa atcccgatct agaggaaggt tatcttttaa
       61 cgttctcttt aaatcaaaaa tcttgtgatt aatttcatct tcttccccat ctaaaatacg
      121 ttcctgacta tcactatttg ttcctttcag tttctcaagg tctcttctgt ttataaatga
      181 cgaaagagcg gaagccatct catcagatga gtcaatatac ttgctattag ccaagccaga
      241 atcaagctct gcatcagcag tctcatctcc atcatctgaa tttgtcattg cattcaactt
      301 atcaatgaat gcagagctaa aaactcctgt atttttaaca tcaagcatac atcacctact
      361 ttatagttag taatttaagt atgaaaccaa tgatttttcg tcttccagca aagtagtttc
      421 agatgggatt atttgtgtag tcttttgtat ttctctttca gatatgaggc ttttatattc
      481 tgcagtatta taataacttg actcctttat ctcacggggt tgaatcagaa aaacccttac
      541 aatattactg atattacttg ttttatactt gaacacatta ccgattacag gaatagagga
      601 gagaaaagga atagatatta tttcgttaga gttcgtttca tgcgtataac cacctatcaa
      661 caaacttttg ccttgtggaa ctctcgctat tgtactgatt ttggtccttc caacttctgg
      721 aaggacagat gtattttcat tattataatt gtaatttgac tgactatttc ctgttccatc
      781 ctctatcgtt aaagacatct caatttgtcc ccgagatgaa aagcgaggta taacatttat
      841 caatgttcca tacgtaacat gctccaacga agaattgcgc tcaccaacta atgacacata
      901 aaaagtcctg ttattatcaa atatagccgg aatattctct tgtgtcaaaa tcaccggcct
      961 tgaaactaca tttgctttct ttttctgatt taatgccata actgaagcaa taaacttatt
     1021 gccgtccaaa gtgcttatcg atgctgatga gctcatatta aatgacgcac caaacgaatc
     1081 accaaatgaa gccgttcctt gccaattaac accaaggtta tttaattcag att
//
LOCUS       DAZPEU010000445         1131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPEU010000445 DAZPEU010000000
VERSION     DAZPEU010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.452"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(403..1098)
                     /gene="aqpZ"
                     /locus_tag="ACHU2L_004481"
     CDS             complement(403..1098)
                     /gene="aqpZ"
                     /locus_tag="ACHU2L_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR1359015.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          318 a          331 c          268 g          214 t
ORIGIN      
        1 ctatcactta aataagtgat actggttgtc tggagattca gggggccagt ctagggattt
       61 ttaatcctat ctgagtgttt agcattgttg cgctcctttc atgtgcaatg ccatataaag
      121 ccactggcgg agggagcgcg cagggggcag ccagtcgccg ccgccccctg cacccccggg
      181 ctctggcgaa caaaatcgcc gctgcgcggt accttcagct tatccccttc ggcaaccggg
      241 tcgggcgcga ggtaacgtcc tgtaagacgc gccctcagcc cacatccatg tgggctgccc
      301 cggccttcgg ggaacgcttc agcgattttg acgccaccaa tacccgtgcg gtttgtaatg
      361 tcgaagataa aacaaccagg gcagataaaa tgccaatgct aattaatcac gcttttccag
      421 cagagtccgg taaatcagac caccgataat gccgccgaca attggcacca cccagaagaa
      481 ccacagttgt tctaatgccc agccgccctg gaagatagca accgcggtgc tgcgcgccgg
      541 gttaacagaa gtgttagtca ccggaatact gattaagtga atcagggtta aggccagacc
      601 aatagcgatc ggcgcaaaac ctgccggagc gaatttgtcg gttgcaccgt ggatcactaa
      661 caggaagccc gcactcaata ccagttcaac taccagcgcg gaaagcatgg aataaccgcc
      721 tggtgaatgc tcgccataac cgttagaagc aaaaccgctg gctgccgcgt caaaacccgt
      781 tttaccactg gcaattaaat acagcagcgc cgctgcaaca ataccgccga caacctgggc
      841 aattacgtag ccaacgactt cttttgccgg aaaacgtccg ccagcccata aaccaatagt
      901 gaccgccggg ttaaaatgac caccagaaat atgaccaaca gcgaaggcca tcgtcagaac
      961 ggtcaaacca aatgccagcg ccacgccggc aaaaccaatg cctaattccg ggaagcctgc
     1021 ggccagtaca gcactaccac agccaccaaa aacaagccag aaagtaccaa aacattcagc
     1081 tgctaatttt ctgaacatat ccacctcaat taaaaattga ccctgtgaaa a
//
LOCUS       DAZPEU010000446         1120 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPEU010000446 DAZPEU010000000
VERSION     DAZPEU010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.453"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..832)
                     /gene="rhtA"
                     /locus_tag="ACHU2L_004482"
     CDS             complement(<1..832)
                     /gene="rhtA"
                     /locus_tag="ACHU2L_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR1359016.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     M"
BASE COUNT          337 a          291 c          276 g          216 t
ORIGIN      
        1 ccattgaagc ggcgatgata gcgccgagcg ccagcagctg tatgggtgtc agtgtttctc
       61 cgaggaaaat catcccggaa acggcagcca gcgccggttc catgctcatc agcgtaccaa
      121 atgtccgtgt tggcaaacgg gtgagggcaa tcatttccag cgaataaggc agagcggtcg
      181 agagaataac gacagccaga cccaatggaa taaccgacca gtgccagagt gcttcaccag
      241 cctgaagcgc tccaattggc acgaaaatta acgctgcaat caacgaacca attgctaccg
      301 tcgcagggcc atgttccgct cctgcgcgtt gcccacttaa aatgtaaata gcccaacaag
      361 ccccggcccc cagtgccagc gcacagccgg ttaaatcgac atgggaaacg tcttgcccca
      421 gcggtagcag gaaccacaga ccaagaaccg ccagcacaac ccagacgaaa tctaccgggc
      481 gacgagaaga gaacagcgcc accgccagtg gtccggtgaa ctccagcgcc accgcaatac
      541 ccagcggtac tgtctgaata gaaagataaa aaagataatt catcccaccc agcgaaacgc
      601 cgtaaaacaa cagcggtaac cgttgctctt tggcaaagcg cagtcgccat ggcttaaaga
      661 acgcgatgag gatcagcgtt cctaatgcca gacgcagcgc agtgacaccc ggtgcgctca
      721 ccagaggaaa aagtgactta gctaacgagg ctccaccctg aatagacgcc atggcaacga
      781 gcaatatgac tattggtaac cagaccggca ttttacgtaa tgaaccaggc atcctttctc
      841 ccacaaatat ctagacttaa gtaaagcgtg gagtgtactg gatataccca atgctggttg
      901 agcatttgtt gaaaaaattt tcccccgttt tgactaaaat gcgccaggat tgatggaatc
      961 attagtctgg tgattaggaa taatctggat gaatgacagg gaaaacatgc gtaatactta
     1021 cgcagttctc tgaaaaagtg atttaaattt agatggatag cggtgtatgg aaacgttctg
     1081 ttacatgaaa tggcccgtta gacatcacaa atcgcgaaga
//
LOCUS       DAZPEU010000447         1109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPEU010000447 DAZPEU010000000
VERSION     DAZPEU010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.454"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..1019
                     /locus_tag="ACHU2L_004483"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACHU2L_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1109
                     /locus_tag="ACHU2L_004484"
     CDS             992..>1109
                     /locus_tag="ACHU2L_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359017.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAZPEU010000448         1109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPEU010000448 DAZPEU010000000
VERSION     DAZPEU010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.456"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            54..233
                     /locus_tag="ACHU2L_004485"
                     /pseudo
     CDS             54..233
                     /locus_tag="ACHU2L_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(250..940)
                     /locus_tag="ACHU2L_004486"
                     /pseudo
     CDS             complement(250..940)
                     /locus_tag="ACHU2L_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1019..1109
                     /locus_tag="ACHU2L_004487"
                     /pseudo
     CDS             1019..1109
                     /locus_tag="ACHU2L_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          299 a          250 c          284 g          276 t
ORIGIN      
        1 tcctattata gaatgacaaa ttaaaattac tgtaaaaaaa cgatacacat ttaatacgag
       61 ggaggccggc agaaaaacgg cttgcggtca ggcaggagaa atgtgtgccg cgaatgaagt
      121 ccttgtatga ctggataaag ctccagagga aaactttgtc aaaccatgct gaaatggtaa
      181 aggcgttcaa ctatatcctg aatcactgga atgcactgaa tgagttttgc cgggtagtac
      241 ttccaatcat tagaatatgt gcttttcaat gaaggaaccg atgacttttt cgtggatttc
      301 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg ggcggagggt
      361 caggttgtta cgttcatacg ttgcgtgaag attttgccgg tcaggtgttt ctcctccggc
      421 acttctctgg cgtagctgcc ccagtcatcg ctggtcacca tgccgataca gaacggagtc
      481 aacagggcta acagttcccg acaggtctca tcattccgtg gcccgcaagt gtaagccaac
      541 acaccgccag ttttagtgtt gtacgcatac cagggccagt gttgttgcgc cttgctgcca
      601 acgaagcgcc actgctcatc aagttcacag ataagggcaa catcagcgtg agcgactggc
      661 gcagatgtta tccgccgggg cgagagcttt ttaatgtccg tatgacggta ttgatgccga
      721 ttttcagtgt cctggcggta tcacggcccc ctgcgccgtt atgtgccatt tcaacgatct
      781 gctccttaac gccgagtttt ctggcttcat aagagtaagt gagctggaaa acacgtttac
      841 atgagcgaca acggaaacgc tcatgccggg aacaactacg gccatgtcga taaacttcat
      901 cggaattaca gcgcggacaa tgaacagtaa cggtagtcat ggaaaacctc aaataccagt
      961 agattaccag aaacttctat cgattgaaga cattaccttt tgccgcaata ggtggggtaa
     1021 cattggtgaa aacgcattac gccgggtggc agttggaaaa aaattatctt ttttcggctc
     1081 tgatcgtggt ggagaaaatg cggcaataa
//
LOCUS       DAZPEU010000449         1101 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPEU010000449 DAZPEU010000000
VERSION     DAZPEU010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.457"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(27..266)
                     /locus_tag="ACHU2L_004488"
     CDS             complement(27..266)
                     /locus_tag="ACHU2L_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR1359018.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(401..1006)
                     /locus_tag="ACHU2L_004489"
     CDS             complement(401..1006)
                     /locus_tag="ACHU2L_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1359019.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          316 a          245 c          188 g          352 t
ORIGIN      
        1 tagagagttt ttttattttt tataagttac ggattactcg ttccggaaat cgttacctgt
       61 tgaattgctg cagcgatagc gtcgaatgca tctttaattg cgcccaggaa gcctgagttc
      121 tgatcgccaa agatgaaagc aagcagagtt gccatagcaa caccaatcaa tgcatattcg
      181 attgcagtaa caccacgttc atccttcgcg aaacgcgcaa agcactgttt agttttacca
      241 tagtacttag taataaaagt attcatgttt tcctcgtata ttattattta aattcaaacc
      301 accagcttca tctgcgtgtc accggtatcc catatttttg ggatgtggct ataattatct
      361 attcccgcta tttagtcttc aaaaaaaatt acatttaata tcatttatct tgattaagaa
      421 atagtaagac taaaaaaaca agcgtcgtca gccagcacac tgctaacacg gtaagaacgc
      481 taataaaaac actccactgc ttcgttctat accattcaat atttttgtca gtatgtttta
      541 ttgggagcag ctcttcagta gagaattcct ggctgttatc attctcagga atgtttgcat
      601 cagtgtttac ttccctggcc tcgctatcgt tggtatagct actactgtct tcatcactgt
      661 cacgctcatt ctcacagaca tgtttctcga cctttcccag atatctgacg ccttttcgcg
      721 gaatggtttt aattagagaa ctcgacaaac ctgcgacgtc taatgccttt ctaagcatgt
      781 aaatctgccc ataataactg ctgtcgctta ccgaaccttt ctgctcccgc caaacctcat
      841 tgataatatg ttgcttatcg gattctccct gtagtaaaag gagtaaaaat cgatagccgt
      901 tttcagacag attgattaca ttgcctgtac tggtggtgat acgtcgagac gctgggtaaa
      961 acttaactcc ctgccctaaa gaaaaataaa aacggtcatc gctcataagt accgacccct
     1021 gttttcttct tcacatgact actcattaca actttgtatg aagtaagatg caaaatcata
     1081 ttgtttacat taagttaagc a
//
LOCUS       DAZPEU010000450         1087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPEU010000450 DAZPEU010000000
VERSION     DAZPEU010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.458"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            228..830
                     /gene="fimB"
                     /locus_tag="ACHU2L_004490"
                     /pseudo
     CDS             228..830
                     /gene="fimB"
                     /locus_tag="ACHU2L_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          339 a          190 c          210 g          348 t
ORIGIN      
        1 gattgataaa tatataaagg tacatagcat gcaagagcat ggcgtttgta tggcaaagtt
       61 attataagta actatcgcta ctccatttaa gttcgctcag aagaactggt ccacttacgt
      121 aagttattaa gcaaacgttc gcttttataa acataatcag gataaaaatg ttggattatt
      181 gctaacccag cacagctagt gcgcgtctgt aattataagg gaaaacgatg aggaataagg
      241 ctgataacaa aaaaaggaac ttccttaccc atagtgaaat cgagtcactc cttaaagcag
      301 caaataccgg gcctcatgca acacgtaatt attgtctgat tttgctttgt tttattcatg
      361 gtttccgggc gagtgaaatt tgtcgattga ggatttcgga tattgatctt aaagcaaagt
      421 gtatatatat ccatcgatta aaaaaaggct tttcaacaac gcacccgcta ttgaacaaag
      481 aagttcaggc tttaaaaaac tggttgagta tccgtacttc ttatccgcat gctgagagcg
      541 agtgggtatt tttatcacgt aaggggaatc cgctttctcg gcaacagttt taccatatta
      601 tctcgacttc tggtggtaat gccgggttgt cactggagat tcatccgcac atgttacgct
      661 attcgtgtgg ttttgctttg gcgaatatgg gaatagatac gcgacttatc taggattatc
      721 ttgggcatcg caatattcgt catactgtct ggtataccgc cagcaatgca gggcgttttt
      781 acggcatctg ggatagagcc agaggacgac agcgtcatgc tgttttatag agatagttga
      841 gataccaggg atggtgttaa aaactacatt attcttacga tatatcaaaa atgattaacc
      901 atttattaat tgatgttatt gtaagtgtga ttcttaatta tcataataaa cattaagtta
      961 accataccca tacaaaatct aatgatttat gttcttcaaa ataaataaac aaaatcattc
     1021 ataagtttac acatcactta aattctcctg tttccacact tttttcttta ttttttaagt
     1081 aactgga
//
LOCUS       DAZPEU010000451         1075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPEU010000451 DAZPEU010000000
VERSION     DAZPEU010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.459"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHU2L_004491"
     CDS             <1..318
                     /locus_tag="ACHU2L_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359020.1"
                     /translation="FSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGH
                     EMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNV
                     ASI"
     gene            321..866
                     /locus_tag="ACHU2L_004492"
     CDS             321..866
                     /locus_tag="ACHU2L_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1359021.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            890..>1075
                     /locus_tag="ACHU2L_004493"
     CDS             890..>1075
                     /locus_tag="ACHU2L_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1359022.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYL"
BASE COUNT          310 a          229 c          282 g          254 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattaccg ctaaccgacg gattaccgcg ccgggttact
      601 ggttttaccg caatgatgaa tttgtctttg actacagact caaagcggaa gatgagcgtg
      661 atgcccttct ggctcaggtc agtgcccgga caggggaatg ggaagaagac ctgctgctgg
      721 ggttaatcag cgacgaagat aaagaaaagc tgaaagcctg tcgtatttac gcgaaatcgc
      781 tgcaggcgat ggatttcagc accatcactg ataaatcctc atacaacgcc attgaatggc
      841 ccgcctctcc ggaaggttct tcctgattta atttatcgcg agaaaaacaa tgtctgtagt
      901 gatatcaggt gcgctgactg atggcgcagg tatccccatg tccggatacc atattattct
      961 gaaatcccgg gtaaacaccc cggaagtggt gatgaacact gttgctgatg tgatgacagg
     1021 aaacgatggt gaatactgtt tccatgcgcg gactggaaaa tatggtgtgt atctg
//
LOCUS       DAZPEU010000452         1069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPEU010000452 DAZPEU010000000
VERSION     DAZPEU010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.460"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(227..652)
                     /locus_tag="ACHU2L_004494"
     CDS             complement(227..652)
                     /locus_tag="ACHU2L_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR1359023.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(649..1032)
                     /locus_tag="ACHU2L_004495"
     CDS             complement(649..1032)
                     /locus_tag="ACHU2L_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR1359024.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          243 t
ORIGIN      
        1 ctcgcgtatg gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga
       61 ttccgcacca cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat
      121 atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca
      181 ggttggcaat acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac
      241 ctccgcagtc ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc
      301 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac
      361 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      421 caatgtaagg gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac
      481 tcagggcatg taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc
      541 ggccatggca ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac
      601 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc
      661 cccctcctca ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc
      721 gtggtgacat tgcctgcagt gccagctggc tttacgtact acgggtaagg tatccgggac
      781 gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca
      841 ctcatgaccg tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc
      901 gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg
      961 tgcagtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
     1021 ttttgcgtac atagtatatg ttcctcagcc gccagacgac acgcaggca
//
LOCUS       DAZPEU010000453         1068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPEU010000453 DAZPEU010000000
VERSION     DAZPEU010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.461"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHU2L_004496"
     CDS             <1..560
                     /locus_tag="ACHU2L_004496"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR1359025.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     GLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMV
                     NEVETALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="ACHU2L_004497"
     CDS             787..>1068
                     /locus_tag="ACHU2L_004497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR1359026.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAZPEU010000454         1067 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPEU010000454 DAZPEU010000000
VERSION     DAZPEU010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.462"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            100..293
                     /locus_tag="ACHU2L_004498"
                     /pseudo
     CDS             100..293
                     /locus_tag="ACHU2L_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            436..657
                     /locus_tag="ACHU2L_004499"
                     /pseudo
     CDS             436..657
                     /locus_tag="ACHU2L_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(678..740)
                     /locus_tag="ACHU2L_004500"
                     /pseudo
     CDS             complement(678..740)
                     /locus_tag="ACHU2L_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          344 a          236 c          226 g          261 t
ORIGIN      
        1 tttagctgaa cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg
       61 gtaattgcag accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct
      121 ttgtgtccga tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag
      181 gtgaagaaag ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt
      241 gccatcgaca gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg
      301 agcggtgtac cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac
      361 tgtccttacc cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg
      421 ttgtaaagtg aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg
      481 ttgcagacgc aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag
      541 cggatggcgg cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag
      601 cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca
      661 ttttcacggg cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg
      721 attttgaact cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc
      781 caaactaacc tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa
      841 tcatcgagga tttcattgca gaatacacat tccactaact ggaagcactc cataagagga
      901 atcagagaaa gtatcgtttt tttatagtaa aattcattgc tgttcaataa aattgatata
      961 gttattaaat tatgaaaata atttcacaaa aaagattaac ttgagttctt aagcatcgga
     1021 ttttagatat tttgaaatat ctggcatcac ataacatcaa aagtata
//
LOCUS       DAZPEU010000455         1055 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPEU010000455 DAZPEU010000000
VERSION     DAZPEU010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.463"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            10..930
                     /locus_tag="ACHU2L_004501"
                     /pseudo
     CDS             10..930
                     /locus_tag="ACHU2L_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          319 a          149 c          221 g          366 t
ORIGIN      
        1 tcttttaata tcgctgtaat tattccttgt tataatgagc agaaggcaat agcaaaagtc
       61 ataaatgatt tcaaaacgaa tattccaact gcttctatat atgtatttga taataattcg
      121 actgacagta cagcacaagt ggcagaggat gctggagcaa cagttcactc tgttcctctg
      181 aaaggcaagg ggaatgtcgt aagacgtatg ttctcagatg tagatgcgga tatttatcta
      241 atggttgatg gagatgacac ttatgatgca agttctgctc cagaaatgat taaccatctc
      301 ataaaaaatc aattggatat ggttgtagga tgtcgtcaag agaatggaga tcaaaataca
      361 tacagaaaag ggcatcgctg aggaaataaa ttgttgactg gtactgttca gactatcttt
      421 aaagggaatt ttactgatat gttgtcgggg tatcgtgtat tttccagacg ctatgttaaa
      481 tcttttccat gtctatcaag gggatttgaa atcgaaactg agcttacaat tcatgcttta
      541 gaattacgta tgaagtatgg tgaggttaat acaaaatatg gcgagcgatc tgaagggagt
      601 gtgagcaaac tatcaacttg gagtgatggg ttcaagattc tgaaaacaat aattaaactt
      661 tactcgcttg aacggccact atactttttt agtattatcg gcgttttact tgctgctttg
      721 tctataattt taggattgcc catcattgtt gattatattg atacaggttt ggtgagaaga
      781 tttccgactg cctttttaac tgcctccatc atgttatcgt caataatggc atttgtatgt
      841 ggcattattt tgcattcaaa tacaacaact agacgtgaaa tgaaggcatt attttatctt
      901 tctgaaaaaa actataaact gataatgtaa atagagtatt ttactatgat tgaattgccg
      961 ttgtatttgt atgttgtgta ttgttttgct tttggaatga cattttttgt cattggtaat
     1021 attgtattgt catctttttt taaggagggg gtggc
//
LOCUS       DAZPEU010000456         1051 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPEU010000456 DAZPEU010000000
VERSION     DAZPEU010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.464"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(108..479)
                     /locus_tag="ACHU2L_004502"
     CDS             complement(108..479)
                     /locus_tag="ACHU2L_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359027.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(540..>1051)
                     /locus_tag="ACHU2L_004503"
     CDS             complement(540..>1051)
                     /locus_tag="ACHU2L_004503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359028.1"
                     /translation="LGKTMRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSP
                     SNYDISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTF
                     AGYYVIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDN
                     GLKIMYLSN"
BASE COUNT          249 a          271 c          250 g          281 t
ORIGIN      
        1 cataccttgg cctaggtaaa gaagatcaga ctcgtagcca catcggtggc aactgagctt
       61 gtacgcaatg cccatctggt tgtctcacct gaatcgtaac gtcagagcta ttcatccatc
      121 cattcggcat caatgtatgc cactaagccg accagctgac cgtttggact gaagtatgtt
      181 gcctgcaccc cacttgacat ctcatcaata ccaccgcact gcgctaggcg ccggtcccac
      241 ttgcccacag aagcgtcagg atcggctgag ttccccaaga cccttgtgag ccatgcctcc
      301 tttaactcaa cctctcgaat gattgcgtcg gccagctcct ccgcgttatc tagttcgaca
      361 tagtctggaa ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca
      421 tacatgacat caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact
      481 agtggttccc ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc
      541 tagttgctta gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca
      601 tttatgacga ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc
      661 ccttttgggg tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg
      721 ttggcaatga cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta
      781 cgatttccgt tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag
      841 ttgtcgtaat ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga
      901 gaaatgtcgt agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca
      961 attggcaggt cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc
     1021 ttcaacagat cgcgcatagt cttccccagc t
//
LOCUS       DAZPEU010000457         1049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPEU010000457 DAZPEU010000000
VERSION     DAZPEU010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.465"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHU2L_004504"
     CDS             <1..318
                     /locus_tag="ACHU2L_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR1359029.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
     gene            complement(365..706)
                     /gene="symE"
                     /locus_tag="ACHU2L_004505"
     CDS             complement(365..706)
                     /gene="symE"
                     /locus_tag="ACHU2L_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR1359030.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(927..>1049)
                     /locus_tag="ACHU2L_004506"
     CDS             complement(927..>1049)
                     /locus_tag="ACHU2L_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359031.1"
                     /translation="AIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
BASE COUNT          280 a          210 c          264 g          295 t
ORIGIN      
        1 ttattcgatc atcttcgcga tgaagttatg cgcctggacg cagggatcac gcaggaagta
       61 ttgaagctat atattgcgtt taaagcggaa acgaattttg ttgatgttgt gccgcaaaaa
      121 agccgactgc gattgtcgct taatatgcag tttcatgaac tggtcgatcc gaaaggtatt
      181 gccaaagatg tgacaaatgt tgggcgctgg ggtaatggcg atgtagaaat tggtttcagc
      241 gacctcgcac aacttcctta cattatggga ttaattcgtc aggcatttga aaaacagatg
      301 gagagcgcgt tggtataacg cggcgaataa tacccggtag cactggcgct accgggtcaa
      361 tgtatcaggc gactttctgt ttacctgtaa ttaccccaat aaattcctgt acctgccttt
      421 gtttacgcgc cgacagctta cacaccttgc gtagtgactg catcagttcg ctctcttcgg
      481 cggcggcggg ttgggcggtg aggacaatac agccttccat cactttgaca tctaccgccg
      541 tgccggtggc aaaaccggcg gcttccagcc actgaccttt cagggtgatg gcgggaatac
      601 ggctgtaatc cgggtaacga ctcgcataac tcacggttaa ttgacgatta tttgccgggg
      661 agacttctgc ttctaacggt tgtgcaatag aatgcgtgtc agtcatgatt actattctct
      721 ataaatagtg attgtgatta gcggtgcgag tgtgttggcg cacatccgca ccgtgctaaa
      781 tacctggata catcatcagt aaatacaggg aaagtccagc taaaaattaa tgaaaataag
      841 atgaaaatta gcgtgaaata taaattttgg tgatgtgaat aaagcggcta tttagccgct
      901 ttattggttt tgtgagggcg agggaattag tttatcgctg cgtcagtgag tgcgtctgca
      961 agatgcagtt gggttttatg ataaatccca atataagaaa taagaccatt gcatactgct
     1021 tcaatagaat ttatcttatc aaatattgc
//
LOCUS       DAZPEU010000458         1035 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPEU010000458 DAZPEU010000000
VERSION     DAZPEU010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.466"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACHU2L_004507"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACHU2L_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="ACHU2L_004508"
                     /pseudo
     CDS             761..913
                     /locus_tag="ACHU2L_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          310 a          205 c          174 g          346 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt atacc
//
LOCUS       DAZPEU010000459         1005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPEU010000459 DAZPEU010000000
VERSION     DAZPEU010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.467"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..104)
                     /locus_tag="ACHU2L_004509"
     CDS             complement(<1..104)
                     /locus_tag="ACHU2L_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFR1359032.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACHU2L_004510"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACHU2L_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR1359033.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
BASE COUNT          276 a          240 c          179 g          310 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacac
//
LOCUS       DAZPEU010000460          990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPEU010000460 DAZPEU010000000
VERSION     DAZPEU010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.469"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            82..>990
                     /locus_tag="ACHU2L_004511"
     CDS             82..>990
                     /locus_tag="ACHU2L_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR1359034.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAZPEU010000461          973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPEU010000461 DAZPEU010000000
VERSION     DAZPEU010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.471"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..700)
                     /locus_tag="ACHU2L_004512"
     CDS             complement(<1..700)
                     /locus_tag="ACHU2L_004512"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1359035.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIG"
     gene            complement(697..>973)
                     /locus_tag="ACHU2L_004513"
     CDS             complement(697..>973)
                     /locus_tag="ACHU2L_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR1359036.1"
                     /translation="AHTSQALANFTGFVVAVSFSFFANARFTFKALTTAMRYMLYVGF
                     MGILSVIVGWAADKCSLPPIVTLITFSAISLVCGFVYSKFIVFRDAK"
BASE COUNT          282 a          231 c          198 g          262 t
ORIGIN      
        1 agcctatata agtccatacg cgaagaggaa aggttgaaaa acttgtgata ccttccagtg
       61 ccagattcca taatttccag ccattaaatt ttgagatgcc agcaacacgc tctgcgcgta
      121 catattcaac gacatccgtc ggaccaccca cccagctcag tatgcctttc atgaaaagat
      181 tgcgctcagg caacagttta atgttctcca caacctcacg agacatgagt cgaaaatctc
      241 cgacattttc ctcgatcttt ggggtgctta ttttgttgtg taatttatag aaccactcag
      301 ctgtcttacg tttcagtcgt ccatcagttg agcggtctga acgtttagca agcaccatgt
      361 cagcacctgc ctgccacttt tcaataagat gagggataac ctcaattggg tcttgcaggt
      421 cgacatcaat aggaattaca gcatcgccgc ttgcatggtc taaccctgca aataaggctg
      481 gttctttacc aaagttgcgt gtaaatgaca gcggaacaac tagcgggtct gaaacagcca
      541 gcgcgttaat aattgactct gtggcatctt tacttccgtc atttatgaat acaatttcta
      601 cttcatatgg cttcaactct tggaattcac gtaccgtttt atagaaaaca ggtatcgctt
      661 cttcttcatt gaagacagga acgacaagag atattttcat ttcgcatccc taaagacaat
      721 gaactttgaa tagacgaaac cgcacaccag actgatggcg gagaaggtga taagagtgac
      781 tattggagga agtgagcatt tatcagctgc ccaaccaaca atcacactca atattcccat
      841 aaatcccacg tataacatgt agcgcatcgc tgtagtcaat gctttgaatg tgaatcttgc
      901 attcgcgaag aagctaaagc tcacagccac tacgaaacct gtgaagtttg ccagagcctg
      961 actggtatgt gcg
//
LOCUS       DAZPEU010000462          966 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPEU010000462 DAZPEU010000000
VERSION     DAZPEU010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.472"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..312
                     /locus_tag="ACHU2L_004514"
     CDS             <1..312
                     /locus_tag="ACHU2L_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1359037.1"
                     /translation="NKKHKQHMDDHLLHLIRSNAIAPTPVYENYFNNTTAFRPKPEDI
                     FKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQP
                     Q"
     gene            574..>966
                     /locus_tag="ACHU2L_004515"
     CDS             574..>966
                     /locus_tag="ACHU2L_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359038.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSI"
BASE COUNT          339 a          156 c          180 g          291 t
ORIGIN      
        1 aataaaaaac ataaacaaca catggatgat catcttctcc atctaattag aagcaatgct
       61 atagcaccca cacctgtata tgaaaattat tttaataaca ctactgcttt cagaccaaaa
      121 cctgaagata tcttcaaatg tggacagacg ctcaaaaaaa ctaaacagaa atttggccat
      181 tatggattag gggtagacca gcatcaaaaa attgataatt ataccatggg gctaaattct
      241 tggaagatag cacctaacga acgtggtgat aaaaaaggtg tcccagggct tagttgtttt
      301 caacctcaat aaaacttcat gagtgctgtg gtatctcttt ttaattttgc cagtatttca
      361 taggttttct taatcgattg tagaggtatg tactgatgac gcctctacaa tatttaggga
      421 ctttctctgc tatgagataa gaaatttcat attatgcttt aaggctgaat gatttgtgca
      481 aaatataatt atttccccat cgaatatgag gtgtatgtca taagataaaa tgttaactat
      541 gtattcctcg cggtgaataa tatgaggtaa aagatgaaaa ttggaactgt ggcaggcact
      601 aatggcagta caacgacaat cgcaacaaat gatatggtgc aagagcatgt taccaacttc
      661 actaaagaac tgtttgggta tattgcaaat ggtataggtg atgatatatc aagtattgcc
      721 aggacaatgc tcggtgaagt tgtggaaaaa attgatgatt ggcaaataga acgtttccaa
      781 caatctattc aagatgacaa aatttcattt accatacaaa cgaatcattc agaaaaatat
      841 agtatgctat cgggcatgcg cgctcatatt ctacgtagga ataactgtta tcagtttatt
      901 gtgacaatta atagtaaaaa ttacggttgc cctcttgata ataccgatat taattggtgc
      961 agcatt
//
LOCUS       DAZPEU010000463          960 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPEU010000463 DAZPEU010000000
VERSION     DAZPEU010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.473"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..167
                     /locus_tag="ACHU2L_004516"
     CDS             <1..167
                     /locus_tag="ACHU2L_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR1359039.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHU2L_004517"
     CDS             152..790
                     /locus_tag="ACHU2L_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR1359040.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
BASE COUNT          256 a          187 c          286 g          231 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
//
LOCUS       DAZPEU010000464          950 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPEU010000464 DAZPEU010000000
VERSION     DAZPEU010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.474"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            5..550
                     /gene="rfbC"
                     /locus_tag="ACHU2L_004518"
     CDS             5..550
                     /gene="rfbC"
                     /locus_tag="ACHU2L_004518"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR1359041.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            566..>950
                     /gene="wzx"
                     /locus_tag="ACHU2L_004519"
     CDS             566..>950
                     /gene="wzx"
                     /locus_tag="ACHU2L_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR1359042.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAIC"
BASE COUNT          284 a          135 c          209 g          322 t
ORIGIN      
        1 taaaatgaac gtaattaaaa ctgaaattcc tgatgtgcta atttttgaac caaaagtatt
       61 tggtgatgag cggggctttt ttatggaaag cttcaatcag aaagttttcg aagaggctgt
      121 aggacgaaag gttgagtttg ttcaggataa ccattctaaa tcaactaagg gtgtgttacg
      181 cggactgcac tatcagctgg aaccttatgc tcaaggaaaa ctggtacgct gtgttgtcgg
      241 tgaagttttt gacgtagctg ttgatattcg gaaatcatca ccaacatttg gcaagtgggt
      301 tggtgtaaat ttgtcggcgg agaataagcg tcaattatgg ataccggaag gattcgctca
      361 tgggttttgc gttcttagtg atgaagcaga atttgtctac aaaacaaata atttctattc
      421 aaaaatgcaa gagcgtggga ttttatggag cgacaaaagt ataaatatag aatggccggt
      481 tcaaaatcca ttgctttctg ataaagatat taatggtcaa aaatttgtag atgctgatta
      541 ttttatatga taaagaaatg taataatgag cataataaaa aatagtgtct ggaacctttt
      601 tggctatgca ataccaactt taattgctat cccctcgcta ggatttctcg ctcgaggttt
      661 ggggcctgaa ggtttcggtg tttatacaat tgcaattgca cttgttgggt atgctggaat
      721 ttttgatgta ggcctgactc gctctgttat tagagaaatt gcaattcatc gtgataatca
      781 tcatgaaaga accaaggtaa tttcaacaag tacatctttt ttggtgctat tttcatgctt
      841 tggtgctttt ttattattga ttttctctga tggaattgtt aattatctaa aaatttctgg
      901 tgttgaacat agtgatatac aactagcatt taaactgttg gctatttgca
//
LOCUS       DAZPEU010000465          949 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPEU010000465 DAZPEU010000000
VERSION     DAZPEU010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.475"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>949
                     /locus_tag="ACHU2L_004520"
     CDS             <1..>949
                     /locus_tag="ACHU2L_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1359043.1"
                     /translation="LVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWF
                     DEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDA
                     EHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIG
                     GMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHD
                     GNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNT
                     CEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFA"
BASE COUNT          245 a          230 c          263 g          211 t
ORIGIN      
        1 accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa gatggcattt
       61 caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt gacacaatcc
      121 acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt cttacccgta
      181 ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg atgtctgacg
      241 ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac atgaccatct
      301 atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca tatcccgagc
      361 atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga atcttccaga
      421 taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc tacgtaattg
      481 gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg tgggataagg
      541 aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca gccgttcagg
      601 cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct catgatggaa
      661 accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac gctggattta
      721 tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag ccaggcatca
      781 ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc tgtgagccat
      841 tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc aagcttaacg
      901 acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgc
//
LOCUS       DAZPEU010000466          932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPEU010000466 DAZPEU010000000
VERSION     DAZPEU010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.476"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(55..243)
                     /locus_tag="ACHU2L_004521"
     CDS             complement(55..243)
                     /locus_tag="ACHU2L_004521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359044.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(540..778)
                     /locus_tag="ACHU2L_004522"
                     /pseudo
     CDS             complement(540..778)
                     /locus_tag="ACHU2L_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          245 a          181 c          216 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aa
//
LOCUS       DAZPEU010000467          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPEU010000467 DAZPEU010000000
VERSION     DAZPEU010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.477"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..200
                     /locus_tag="ACHU2L_004523"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACHU2L_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="ACHU2L_004524"
     CDS             300..>923
                     /locus_tag="ACHU2L_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR1359045.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAZPEU010000468          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPEU010000468 DAZPEU010000000
VERSION     DAZPEU010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.478"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            101..>923
                     /locus_tag="ACHU2L_004525"
     CDS             101..>923
                     /locus_tag="ACHU2L_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1359046.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
BASE COUNT          309 a          228 c          135 g          251 t
ORIGIN      
        1 ccagaagttc ctgtctactg ctgaagggaa ccaataacat tgcaaagcag ataaaaaaga
       61 atccaacacc tctcaacata aagaatacac cctgatattt atgctcccga caaataacaa
      121 tcacagatta atttcaaatt cgttctccac ttattcaatc gacactagcc gcgcatatga
      181 aaattatcta acccattgga ctgaatggaa aaataaccgc atacaagaag aacaacgaga
      241 catcgctttt cagcgactag tatcatgtct acaaaaccaa gagacgaacc tggacttgtc
      301 tgaattaggc ctgacaacat tacctgaaat ccccccggga attaaatcaa ttaatataag
      361 taaaaataat ttaagcttaa tctccccatt gcctgcgtcc cttacacaac ttaatgtcag
      421 ctataacaga cttattgaac tgcctgcttt gcctcaagga cttaaattat tgaatgcgtc
      481 ccacaatcaa ctaatcacac tacccacact ccccatatct ttgaaggagc ttcatgtctc
      541 aaataatcaa ttatgttctc ttcctgtttt accagaacta ctggaaacat tagatgtatc
      601 atgtaatggg ctggcagttt taccaccttt accattttct ttacaagaga ttagcgcaat
      661 agggaatctt cttagtgaac tcccccctct acctcacaac attcactcca tatgggcaat
      721 cgacaatatg ttaaccgata ttccatacct gccggaaaat ttaaggaacg gttattttga
      781 cataaatcag ataagtcata tcccggaaag cattcttaat ctgaggaatg aatgttcaat
      841 agatattagt gataacccat tgtcatccca tgctctgcaa tccctgcaaa gattaacatc
      901 ttcgccggac taccacggcc cgc
//
LOCUS       DAZPEU010000469          921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPEU010000469 DAZPEU010000000
VERSION     DAZPEU010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.479"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            342..>921
                     /locus_tag="ACHU2L_004526"
     CDS             342..>921
                     /locus_tag="ACHU2L_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1359047.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKL"
BASE COUNT          324 a          183 c          151 g          263 t
ORIGIN      
        1 gggtttgtta ctaataactt ctgaaaacag aaaaaaatag agcagtaatt ctatttcaga
       61 gaggagtaac tccatctatc ctcgatacgt actgaacgtt atcgtgatat attcatcatg
      121 ctttcttcac cattattcga gtatagggag agacgcaatg accttaaata cctttttatg
      181 gctgaattac cattctcagg aataacagca gactccttcc catagacggc ttagggggaa
      241 agcaataccc ggagaaaggg tacaaagcaa actgttttaa aattaataag ccatattcta
      301 tttaactaga attcttatcg ttgataagaa ttctagttat aatgattaaa tcaaccaata
      361 tacaggcaat cggttctggc attatgcatc aaataaacaa tgtatactcg ttaactccat
      421 tatctttacc tatggaactg actccatctt gtaatgaatt ttatttaaaa acctggagcg
      481 aatgggaaaa gaacggtacc ccaggcgagc aacgcaatat cgccttcaat aggctgaaaa
      541 tatgtttaca aaatcaagag gcagaattaa atttatctga gttagattta aaaacattac
      601 cagatttacc gcctcagata acaacactgg aaataagaaa aaacctatta acacatctcc
      661 ctgatttacc accaatgctt aaggtaatac atgctcaatt taatcaactg gaaagcttac
      721 ctgccttacc cgagacgtta gaagagctta atgcgggtga taacaagata aaagaattac
      781 catttcttcc tgaaaatcta actcatttac gggttcataa taaccgattg catattctgc
      841 cactattgcc accggaacta aaattactgg tagtttctgg aaacagatta gacagcattc
      901 ccccctttcc agataagctt g
//
LOCUS       DAZPEU010000470          898 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPEU010000470 DAZPEU010000000
VERSION     DAZPEU010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.480"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(153..>898)
                     /locus_tag="ACHU2L_004527"
     CDS             complement(153..>898)
                     /locus_tag="ACHU2L_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1359048.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          245 a          200 c          190 g          263 t
ORIGIN      
        1 gtttaaatta acatattttc caatataaaa aataagactc tggcttcaat tgtgcgcgga
       61 ttttcttaca ggtgtaggat aatagccttc acaaaataat tatccgcgca attttcgtga
      121 atggagcgca tagactaagg tcaaaatcac aattattcat agataaaagt gacaccaatg
      181 actgactgga cggttccggc tgaaaccttg tttcctgttt gtccataggt tgcgcttaaa
      241 cccagaggta ctttgctttt atttactgtc cccagggaga cattttctcc cgtagccagt
      301 atttttccat tacgcattag agtgacaccc actcccgatg cttttgttgc atcaggtgct
      361 gtgttagcaa acacttgtcg gctactgtca gtggtggcac ccgaaagata aaatgagagc
      421 ttttgttcgc ttgagcaata tacgccaagg ggtatttctg cgcttccagg aaagtccggg
      481 agatccacgg tgacattacg actatcaacg gtgcagccgc ctgttggcat aacaacactg
      541 ttatttgaaa taatattcca cgtaaaatta cgcggatttc cgcttcctaa tgttgcaatt
      601 ttatacatat gaatacgggc aatgacttcc cccgctttta tgacaactcc accggccgcc
      661 ccaactgggg taatatagag ttttaacggt aatggcatcg gtgtcttatc accaatatcc
      721 aatacgttgg tatttgtcgt caacgggaag gggtaggtca cattattcca gtacagcgat
      781 cctttatatg actgtaggga gcctgcgaaa gctgagcctt gtaccaggtt tatatgatca
      841 gtgtcgtacc agccgccgta atcattccag caacttatat gctgagacaa gtctacaa
//
LOCUS       DAZPEU010000471          896 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPEU010000471 DAZPEU010000000
VERSION     DAZPEU010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.481"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(243..725)
                     /gene="sseA"
                     /locus_tag="ACHU2L_004528"
                     /pseudo
     CDS             complement(243..725)
                     /gene="sseA"
                     /locus_tag="ACHU2L_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT          213 a          245 c          213 g          225 t
ORIGIN      
        1 gactaataag ccaggttatt ttattaccga acacggcgga aagggccggt gtcgataata
       61 cgctaaataa acaataatta ctctcttttg cttgacaaaa agagagttac tggtgagtat
      121 tgttttgctg tgaagcacct gcgttgctca tgtaacacaa gtcaacctgc tggaaaggcc
      181 gcgaaccaga ccagcaataa aaaaacgcca aattcggcgg ttttttattg cctgtaatat
      241 acttatttca ctggctcaac cggtaaatct gcccgcgcgc cccattcact ccatgcgccg
      301 tcgtacagtt tcacgtttgg cacatccagc gtcgcgagtg ctaacaaaac cacggctgcc
      361 gttacaccag agccgcagct gacgataatc ggtttgtcat agctgacgcc gcgaccaaaa
      421 aatatcgcat ccagttcatc ggtcgttttt agttcgcctt cgcgcaccag ttccgtccac
      481 ggaacgttca atgcaccggg aatatgtccg cgacgtaaac ctgggcgagg ttcatcaact
      541 tctgcgttaa aacgtgcagc cgggcgggca tcaataattt gcgccgtatt ttcatggctt
      601 gccaataaca catcggttac tttcaccact gcttcaggat taaacgcggc gttaaactct
      661 ccttccggca gctctactgc accttcttct aatagcaggt catcgcgctg ccagcctgca
      721 agtccccgtt actttattcg taccccttat aatggggtgt tagccagcca gacccggcat
      781 gattactgcc cccagtcgtc catgatccgg ggggggatgt caccgggtct ggtggggcgc
      841 tggtaaccgc taataggggt caggtcaggc acttttgccg ggaccgtctg taacgt
//
LOCUS       DAZPEU010000472          895 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPEU010000472 DAZPEU010000000
VERSION     DAZPEU010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.482"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(153..803)
                     /gene="zinT"
                     /locus_tag="ACHU2L_004529"
     CDS             complement(153..803)
                     /gene="zinT"
                     /locus_tag="ACHU2L_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR1359049.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          270 a          167 c          164 g          294 t
ORIGIN      
        1 cctttttctc agtttttttc tacagacata ataaaaaaga cacgactcaa gtatgagcgt
       61 gtctgagttt gtcttactta tttgtcttgg taaattattg ttgttactac caggcactgt
      121 tctgttaaat aactgaagtc agcgcaaaaa aatcaatgag acatcatctc ctcgaccact
      181 tcttcgctac tcaactgata gggataatac gttggccagt tttccatttc attcaataaa
      241 gattgctggg aatcattacc cataaaaatg tgaaaatgag atgattttcg tggtgcaatt
      301 atatgatcgc taaattgtat atatttaggg gctttgcttt caggatcttt acattcgaat
      361 aagtaacgaa cgcctttctt gcctgattta taggtgagta ttttgtatcc atcgtaatca
      421 tatttacagg atgttgtttc attatttcta tggaattcga caatgccgtc ctcaatgcca
      481 atcatttcga tatctgtcgg ataacctttg cgataataat ctttaatttc agcaaatgtt
      541 ttggttttat ctgcatccgc tttcttctga aagacgggat caagtttgcc actttgcagt
      601 aaagggtaaa cggattgcca gactccatcc cagtcactga gcgttcggtt ttgtacattg
      661 gtatcatcaa aaacaccatt agcagctttt tgttcgacct ctgttaaggg tttgccatga
      721 gaatgatgac catgcgaaaa ggcaggagcg ctaacaataa agacacctaa agcaacagcc
      781 agtttatgaa gacgaatcgc caaaacaatt tcctccagag ttaatgtaca ttatgtgtaa
      841 tgttatattg taacatatga aaaaatgcaa caactcatta ggaaatgaga gcatg
//
LOCUS       DAZPEU010000473          893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPEU010000473 DAZPEU010000000
VERSION     DAZPEU010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.483"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(167..568)
                     /locus_tag="ACHU2L_004530"
                     /pseudo
     CDS             complement(167..568)
                     /locus_tag="ACHU2L_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          233 a          202 c          188 g          270 t
ORIGIN      
        1 tatattttag taaagaattt attttttaca ttatagtcgg ctaaatgaag tttactgtaa
       61 aaaaacgata cttttttcgt aagcgatgag atgtggagcc agaaaaatgg ctgcgttacg
      121 tcattgaact gtccttgacc cacgatattc agacagtcgt tgtatcttaa gttgacatta
      181 cccgctgacg tcgatattcc cttggtgaga gataacccaa cgcactatgc ggatggtttt
      241 cattgtaatg ctcgatcgcc actgcaaggt tatgcaatgc cgttcttggt ttcggcatga
      301 acgcgatgca gtcttccttc atcgttttca tgaaccgttc tgctattcca ttactctgag
      361 ggctactgac tgccgtcgtg cagggctcca gattcaactc tctggcgaac tgccgtgttt
      421 catgagccgt ataagccgaa ccgttatcgg ccactggatt ggccgttccg gtcctttatc
      481 gccatatcgc tcctgacatg acatcctgca ccgttgcttt gtcataatct cccgtgcctg
      541 ctgcccagtc tatggcttca cgatcgcaat atggaacgta acgctgtcat cgggagaatc
      601 gtcaatgcgg cattcggtta acgcttacca atatcaacac caaccggatt tgtggagcct
      661 ccagtctgaa ggggctttat gcgttccgta taggatatta tgatgctgga gttttgcgaa
      721 gctccactcc tttccgaaca tttacacaga tattgcctaa gaaaagtgaa accataatat
      781 ggttatagtc cctttcagtg caaatactta gcttgttgca cagagaaata gaagctgcat
      841 aagctctttc ttcaaaaaat gtaaataaag ttaaatatag gaaaaattac tta
//
LOCUS       DAZPEU010000474          870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPEU010000474 DAZPEU010000000
VERSION     DAZPEU010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.484"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>870
                     /locus_tag="ACHU2L_004531"
     CDS             <1..>870
                     /locus_tag="ACHU2L_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1359050.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZPEU010000475          857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPEU010000475 DAZPEU010000000
VERSION     DAZPEU010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.485"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(26..709)
                     /locus_tag="ACHU2L_004532"
     CDS             complement(26..709)
                     /locus_tag="ACHU2L_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1359051.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT          227 a          154 c          164 g          312 t
ORIGIN      
        1 cttgttataa gcgtactaac tgcatttatt ttttgcttct tttctgccga ttttcagcag
       61 agggaagatt gtcgaactga atattttcca tgttgatggc taacttcaga tatcccaggt
      121 tattgggcga tacacccttc agggtcaaat taatgtcttg ccattgacct ggtaataatt
      181 tcaatgtgga tttcattgat gtgttgaaaa gtaattgttg ccaccgggca aactctttgg
      241 catcgacagg tttttcacca ccccatgtcg cattaaaacc aatgtttgag ataggtgtac
      301 ttaataagtt gaccagtgag atatgaataa cactaccatt ctctgtggcg tcaatggata
      361 acactcgacc cgctaaagcc accgaaccgt tgttcaggat gaaatacttc gtttttgctg
      421 ggttaaactc ggtaaaaata aaagtatttt gttggtcttt aacggattta attacgtgag
      481 ttaaagtctt tgtactgttt attatgccag tttgttgttt ttgaatagtt tcgagttgac
      541 tctttagctg tgcttgttgt ttttcaagta gcacaatttt attattatgt tcttcttgtt
      601 gtttcagtaa actactttct ggcttgcctt cttcaatgta gtcacatccc gttaatgcgc
      661 agagaactga aacagtgaca gcactgaaaa aaatcttctt caagttcatg taaaaatcct
      721 tatttcctcc gaccagcgct caatgccggt agagttgtcc attgtattat tggtgagcta
      781 tgttgcttta cattaatgac agtgatgtgt gtcgattaaa tattgtttta agcattcatc
      841 ataaaaatgt atcattc
//
LOCUS       DAZPEU010000476          852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPEU010000476 DAZPEU010000000
VERSION     DAZPEU010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.486"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..763)
                     /locus_tag="ACHU2L_004533"
     CDS             complement(<1..763)
                     /locus_tag="ACHU2L_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1359052.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT          288 a          176 c          126 g          262 t
ORIGIN      
        1 agtcatataa tagcacctct gaacaaattg cataagtttc gtcattagag gattttatac
       61 atcttgataa ataccgctgc ccttccccaa gcaaataatg catactattt acatcatata
      121 taaaatttgc acctggcaca cagggtaaat gctttttcga aatttcagcg gcctctattt
      181 gagatttagc gatgccaatc tcgttaagat tctctgaaag atataaatca caggtgtcaa
      241 tcttacctga tttaaccaat ggataatccg ttatactgta ggtatcttta aaagccacgg
      301 caaacatttc aataataaca attactacca caagcgaaat caaaatgcga aaaagaggaa
      361 tactcctttt gcgctctact ttttcaacaa catcttcttg taaatgagaa tcatcataag
      421 attgtttact tattgttatg ttttcactga tgtaaaagcc tactttgggt atcgtaagaa
      481 tcacattttc ttcaacacca atatttttaa aacttttgcg taacagggat atatattggt
      541 tcaatgtgtt gtttgacaga tcaaatgaaa atctctccaa aacgctatca atgattgagt
      601 tcctgtcaac cgctccacca ttactttcca gtaacactgc caaaatacac cccgccgtag
      661 ctggaaaatt tatctcatcc tgggtattta cattcgagag cgagcatttg ttcggatcaa
      721 aaataatcgc gtcattgata atgaaaaaac atctccgctc catgcaggca acaacacaaa
      781 ataagatttt gtcattttag cctattcctg gtggaactga cacaagaaac taaaatacaa
      841 atataaccct ga
//
LOCUS       DAZPEU010000477          851 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPEU010000477 DAZPEU010000000
VERSION     DAZPEU010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.487"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            59..556
                     /locus_tag="ACHU2L_004534"
                     /pseudo
     CDS             59..556
                     /locus_tag="ACHU2L_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            597..788
                     /locus_tag="ACHU2L_004535"
                     /pseudo
     CDS             597..788
                     /locus_tag="ACHU2L_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          219 a          210 c          250 g          172 t
ORIGIN      
        1 gcgccagccc cgtcccgccc gatagagcgc ggcatagcgg gccccggatt acttgcgtgc
       61 gacggcagaa gaagccctga agcgaatcag tgaactgtac gccatcgagg atgaaatacg
      121 gggattacca gagtcagagt gtcttgcagt caggcagcag cgaagcaaag cgttactgac
      181 gtcgctgcat gaatggatgg tggagaagaa tggcacgctg tcgaaaaaat ccagactggg
      241 cgaagcattc agctatgtac tgaatcagtg ggacgccctc tgttattaca gtgatgacgg
      301 tctggcggag gcggacaata acacagcgga aagagcgctt cgtgcagtct gtctcggaaa
      361 gaaaaattac gtgttcttcg gtagcgatca cggcggcgag cgtggtgcac tgctgtacgg
      421 gctgatcggc acctgccgtc tgaacggtat cgatccggaa gcgtatctgc gccatattct
      481 gagcgtactg ccggaatggc cttccaaccg agttgacgac ctcctgccat ggaaggtggt
      541 gctaccatca ggataatcgt caatacggtg ttcggtgaac gtttacttgg agcatcatgc
      601 ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag
      661 ccttcccggc ggtgaaaatg ttcgaagagt atgacttcac gttcgccacc ggagcaccgc
      721 agaagcagct ccagtcgtta cgctcactca gcttcataga gcgcaatgaa aacatagtct
      781 tactggggac atctgacatc acaaatcccc gggtggggat ttgtgtataa gagacagccc
      841 atggaggaag g
//
LOCUS       DAZPEU010000478          848 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPEU010000478 DAZPEU010000000
VERSION     DAZPEU010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.488"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..744
                     /locus_tag="ACHU2L_004536"
     CDS             <1..744
                     /locus_tag="ACHU2L_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359053.1"
                     /translation="MCNLSQETILTLLAAVGANNVSGLCMSFMNGHVDTIKAYGEIVF
                     KTPLTSDKRLYLLAAKDSHDLPGLFFALQNGHADSIRMFGSLLNKKMLSSEQIKELLK
                     VKHGLFMALQNGHTKAIMAYGDILKILPPHQEYIDELLWIKNPNGTSGLFMAFYNGHT
                     ETIRAFCNILKNYSFTTRRLVEMLSATNKDGIPGVFVSVVNRDKETILEYCRIIKENN
                     LEPDTIAEQFSKKMKKTFIEIINRFNHFL"
BASE COUNT          301 a          132 c          162 g          253 t
ORIGIN      
        1 atgtgcaatc ttagtcaaga aacaatactt acattgcttg ctgcggtagg ggcaaacaat
       61 gtgtcgggtt tatgcatgtc ttttatgaac ggacatgtag acacaataaa agcctatggg
      121 gaaatagtct ttaaaacccc gttaacttcc gataagagat tatatctatt agctgcgaaa
      181 gacagccatg atttacctgg gttatttttt gcattgcaga atggacatgc agatagtatt
      241 cgtatgttcg gaagtctgct taataagaaa atgcttagta gtgaacaaat taaagaatta
      301 ttgaaagtaa agcatgggtt gtttatggca cttcaaaatg ggcacacaaa ggccattatg
      361 gcgtatggag atatactaaa aatactcccc cctcaccagg aatatataga tgaattatta
      421 tggataaaaa atcccaacgg tacatcagga ctttttatgg cattttataa tggccatacc
      481 gaaacaatac gtgccttttg taatattcta aaaaactata gttttaccac aagacgattg
      541 gtcgaaatgc tgtccgcaac taataaagat ggtattcctg gtgtatttgt atctgtagta
      601 aatagagaca aagaaacaat attagagtat tgtagaataa taaaagagaa caatctggaa
      661 ccggatacta tagcagaaca attttcaaag aagatgaaaa aaacgtttat tgaaataata
      721 aatagattta atcatttttt atgagactgt aatttaaatt gtgtaattac ctgtttttga
      781 tatgttcact ccaacaaggt gtagtgaccg ggtaactgcc aactttcagg caaccacctc
      841 aattggca
//
LOCUS       DAZPEU010000479          846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPEU010000479 DAZPEU010000000
VERSION     DAZPEU010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.489"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..481
                     /gene="virA"
                     /locus_tag="ACHU2L_004537"
     CDS             <1..481
                     /gene="virA"
                     /locus_tag="ACHU2L_004537"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR1359054.1"
                     /translation="IINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTSAFWQSLHIT
                     SSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAISASGGRAYE
                     VSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVSFAIISPDV"
     gene            660..>846
                     /locus_tag="ACHU2L_004538"
     CDS             660..>846
                     /locus_tag="ACHU2L_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1359055.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT          265 a          194 c          161 g          226 t
ORIGIN      
        1 aataatcaat tcaaacttcc cccaacaaga cctatgtttt caaacagaaa aacttttgtt
       61 tacttcactc tttcaagacc ctgcatttat atctgcacta acatctgcat tctggcaatc
      121 tcttcacatc acgtcttcct ctgtggaaca catatatgcc caaattatgt ccgaaaacat
      181 agaaaacagg cttaatttca tgcctgaaca acgagttatt aacaattgtg gacatattat
      241 aaaaatcaac gcagttgtcc ctaaaaacga cactgcaatc tctgcctctg gggggagggc
      301 ttacgaagtt agctcatcaa tattaccatc gcacataaca tgtaatggtg ttggtattaa
      361 taaaatagaa acaagctatc tcgttcatgc tggaacactt ccaagcagtg aagggcttcg
      421 caatgctatc cctccagaaa gtcgtcaagt atcatttgcc atcatatctc ctgatgttta
      481 agccaatatt atattggctg ccagactgta aaaaaacgtt cttcagaaca gctctataaa
      541 ctattaaacc aaagctaaaa ggatggggac caatagaatc tcagcccatc ctttttatat
      601 cattatgaac cgccccggaa atcctggaga ctaaactccc tgagaaagag gtaaacagga
      661 tgactaaaaa tacacgtttt tcccctgaag tccgtcaacg ggcagttcgt atggttctgg
      721 aaagtcaggg cgaatatgac tcacaatggg cagcgatttg ttccattgcc ccaaagattg
      781 gctgtatact ggaaactctg cgtgtctggg tacgccagca tgagcgggat accgggggcg
      841 gtgagg
//
LOCUS       DAZPEU010000480          840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPEU010000480 DAZPEU010000000
VERSION     DAZPEU010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.490"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            46..800
                     /locus_tag="ACHU2L_004539"
                     /pseudo
     CDS             46..800
                     /locus_tag="ACHU2L_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          248 a          177 c          205 g          210 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cacgtatagg aaattgaaaa actaagcgat acccctgtcc
//
LOCUS       DAZPEU010000481          839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPEU010000481 DAZPEU010000000
VERSION     DAZPEU010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.491"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            147..>839
                     /locus_tag="ACHU2L_004540"
     CDS             147..>839
                     /locus_tag="ACHU2L_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1359056.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLH"
BASE COUNT          313 a          167 c          117 g          242 t
ORIGIN      
        1 taatctggaa gcgcaaaata atagcctaaa aaaatgatat ttctgatagt tataaactga
       61 aatttcttaa tcttgattat aacaagctag catcgctgga tgtctcccgg caagaatctc
      121 tgattgagtt aagtgcccat cacaatatga tcactgacct tatattacac aatcatccca
      181 ggatgaaaac aatcacttta aacgacaacc atattgcaca tttaaacgcc aaaaacacta
      241 caaaactgga atatttaaac ttaagcaata acaatttact gccaaccaat gacattgatc
      301 aactaatatc atcaaaacat ctttggcatg tattagttaa cggcatcaac aatgatccac
      361 ttgcacaaat gcagtactgg actgcagtaa gaaatataat tgatgacact aatgaagtga
      421 ccattgattt atcaggactt aatttaacca ctcaaccacc agggctgcaa aacttcacct
      481 ctatcaatct tgataataac caactcacac attttgatgc aaccaactac gatagactcg
      541 taaagctaag tctgaatagt aatgctcttg agtcaataaa ttttcctcaa ggcagaaatg
      601 taagtattac acatatatct atgaataata atgctctcag aaatattgat atagataggc
      661 tttcatcagt tacttatttt agtgcggcac ataatcaact agagtttgtg caattagaat
      721 cttgcgaatg gctgcaatac ctgaatctca gccataatca attaactgat attgttgcag
      781 gtaataaaaa tgaactctta ctgctggatc tatcccataa taaactaaca agtttacac
//
LOCUS       DAZPEU010000482          832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPEU010000482 DAZPEU010000000
VERSION     DAZPEU010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.492"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..327
                     /locus_tag="ACHU2L_004541"
     CDS             <1..327
                     /locus_tag="ACHU2L_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1359057.1"
                     /translation="VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNG
                     LLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVI
                     LKITYN"
     gene            complement(370..570)
                     /gene="glgS"
                     /locus_tag="ACHU2L_004542"
     CDS             complement(370..570)
                     /gene="glgS"
                     /locus_tag="ACHU2L_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR1359058.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          155 g          241 t
ORIGIN      
        1 gttagtgaga cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg tactgagcaa
       61 ttcaatattt tagggagctt tttcactacg gatattttga gtgattatag ccatctggat
      121 atgggtaatg gactgctatt gaagatattt cataaagatg gaacagcaac ggaatttaac
      181 cgcttctcac aatttgcttc tttttcatcg tctagtgcac cttcggtcac cgcaccattc
      241 agggcagaac ttagtgcgaa cccggcagag actgttgttg agggaccgtt tagtaaagac
      301 gtaatcctca aaatcaccta taactagcat ctaatacaaa cactaaaacg ggccatcagg
      361 cccgttatat cagtgctcta actccagctc tcttgcctgc atcatctgtt ggcaatacca
      421 ggcataacgt gcgcaaaacc aagttctatg ttcttcatcc atgttaccag taacagccag
      481 aatatcgacc gggcgacctt ctgcgtgcat tctggcaaaa ctacgcgcca ggaaatcgaa
      541 attattcaaa gaattaagac tatgctccat aagcacatcc tccggttatc gcataccttt
      601 taaggccata tgctttttct gatttcagta ttccccaatc agtgtagtgc ctttaaacat
      661 aacgtgcgta aatatattgt ccccgatcac actttttagt gaaaaataaa aaaccatatt
      721 aaaatacttt ctcttccaga acttctctaa catgtgttat tattaacaaa gacaaatgta
      781 agcagggaaa ctattccaag gtaaatattg cgtcactaaa tggacattgg ag
//
LOCUS       DAZPEU010000483          827 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPEU010000483 DAZPEU010000000
VERSION     DAZPEU010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.493"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..534
                     /locus_tag="ACHU2L_004543"
                     /pseudo
     CDS             1..534
                     /locus_tag="ACHU2L_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          225 a          200 c          185 g          217 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatacaaa aacaaataat
      721 accgttgcaa taattccttt tagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 aaaaattgta caacatatta aaatatgttc atttcgttat aatacac
//
LOCUS       DAZPEU010000484          818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPEU010000484 DAZPEU010000000
VERSION     DAZPEU010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.494"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>818
                     /locus_tag="ACHU2L_004544"
     CDS             <1..>818
                     /locus_tag="ACHU2L_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1359059.1"
                     /translation="LERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYTIGSKIANV
                     IFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIALMSTFYDS
                     DNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTRFSAFQDTV
                     FNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLV
                     NLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENGSLGD"
BASE COUNT          276 a          117 c          155 g          270 t
ORIGIN      
        1 acttgaaaga caacagtttc ttttcccact ggactatgag aataaattac aagaacaatt
       61 agataagtat tttacgattg aatcgcaccc attattatac aggtatacca ttggtagtaa
      121 gattgcgaat gtgatttttg aaaagttata cagtcgtatt gactttaaca aagaacaata
      181 tatatccttt ataaaggacg catttattca tttttatgat tatagcaggc gatatgctat
      241 tagtgagaat atcgataaag acgctgtaac aaacaatatc gcattgatga gcacttttta
      301 tgacagtgat aatacgtcag gtgaggtttt aaataatgat tttacagaag aagagtcatt
      361 tgaaactgcg ttagatgtag agcatgcaat agtgcttgga tttgctgatg ataattttga
      421 aacaaaacct gttcattatc aggatttatt aacacgattt agtgcctttc aagataccgt
      481 gtttaatctg tttcctgaaa tgcactcttc gcattaccac gatatttgta gcgtaagtgt
      541 ggatatgaca aagggaacgc aatgcatgat tcacttgatg gtgaatgaag aagttttcat
      601 gtctctgcct gttccagttg ccacgatggt acgtgaagat gcaagtaatt tagtcaatct
      661 taaaacacta ctcaatgatg gttgtttcat taaatatagt cattttaatg acgtggcatt
      721 aataaagcaa aacataagta atctctattt atcacacact gttgtgaatg aaagcatatt
      781 gaaaaaatgt tgttttgaaa acggcagttt aggtgatg
//
LOCUS       DAZPEU010000485          817 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPEU010000485 DAZPEU010000000
VERSION     DAZPEU010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.495"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            77..817
                     /locus_tag="ACHU2L_004545"
                     /pseudo
     CDS             77..817
                     /locus_tag="ACHU2L_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT          291 a          149 c          150 g          227 t
ORIGIN      
        1 aagtgatgca agaatactcc taagtaaatg aaagcgcacc acatgaaatt atcaacacat
       61 gtaatttcat cattaggtgc tatcgaaaat aactcaggag ctacttaaga tgaataatgt
      121 cttactcatg agacccgatg actcagaatt tatgatacct gatgactggt cagggctaaa
      181 cgcaaaaggc attacatacg aacaagataa tagagtgaca gagtcttttc tgccttttaa
      241 atctttttat tgttcaccgg tgtgcacgac agttgaactc atctcataaa ataattgttt
      301 tgctatgggt atccgctacc ttctggaaaa gaaaagagac ttaaggctaa atcactttaa
      361 agccttctct cataaatgcg agacaaaacc tgacgtgtta attatgaccg ggtgcaccgt
      421 cgctgatact tatagtatat tctcacgcat tgttgaagaa aatataatga aaacgatgac
      481 acctatcgtc atcattactg ggcatcactc agaagatctt tgttctctgg catcgaaact
      541 gggaggagtt aaaataatat caccgacaga aaagattgca aaaatagaaa acgctatcaa
      601 tgatgctttt aacagcaagc ctaaaaaaat aagattttta acagatatac tctctcctca
      661 acaaattgag accatgaaaa tggttgcgca gggtagatcc gttaatgaga tttcagccgc
      721 aatgaatatt agcatcaaga ccgtgtatgc acataaagtt cgtgctatca ataagctggg
      781 aattaagaaa aaagtttatg aagcgctttt ttataaa
//
LOCUS       DAZPEU010000486          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPEU010000486 DAZPEU010000000
VERSION     DAZPEU010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.496"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            103..>789
                     /locus_tag="ACHU2L_004546"
     CDS             103..>789
                     /locus_tag="ACHU2L_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1359060.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT          261 a          133 c          172 g          223 t
ORIGIN      
        1 ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca catgttaacg
       61 atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg taaatgggcc
      121 aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta tgcaaaattc
      181 ggtgtagaaa tctatgctgc tgccaaacaa ggtgaacccg atccagaatt aaacacatct
      241 ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt tattgataaa
      301 gcaattgata aagccaaagg cggcggagat gaaacgttcg tacagggacg ttatgaaggc
      361 tttggtccta atggctcaat gattattgct gagacattga cttcaaatgt taaccgtacg
      421 attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc ggcaggttct
      481 gtcagctata tgtttgacaa tacgggtgtg attgtattta aaggaacaga ccctgaccat
      541 atttttgaaa ttttgcttga agctgaagtt gatgttcgtg atgtaactga agaagaaggt
      601 aatattgtta tttatactga acctgctgac cttcataaag gaatcgcggc gctaaaagca
      661 gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc tgaagttgag
      721 ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga agatgacgac
      781 gatgtacaa
//
LOCUS       DAZPEU010000487          784 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPEU010000487 DAZPEU010000000
VERSION     DAZPEU010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.497"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..285)
                     /gene="mxiI"
                     /locus_tag="ACHU2L_004547"
     CDS             complement(<1..285)
                     /gene="mxiI"
                     /locus_tag="ACHU2L_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR1359061.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLL"
     gene            complement(298..549)
                     /gene="mxiH"
                     /locus_tag="ACHU2L_004548"
     CDS             complement(298..549)
                     /gene="mxiH"
                     /locus_tag="ACHU2L_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR1359062.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(557..>784)
                     /locus_tag="ACHU2L_004549"
     CDS             complement(557..>784)
                     /locus_tag="ACHU2L_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFR1359063.1"
                     /translation="ENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIEFSVLLI
                     DDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
BASE COUNT          235 a          146 c          109 g          294 t
ORIGIN      
        1 taataaagtt tcaacagccg aaactgtttt tcttgctaac gtgccagcta atgatactcc
       61 tattgaataa tttgagagcg tcgtctgtaa cttggccaaa gattctgggt ttaatgattc
      121 tggattgctt accatctcca ttatttgtga tacttgaata tctgtatcca tcttaatatc
      181 actatattta gccgacacga cgtcttccag acttgatatc tcttgagatt gaaaatcact
      241 ggctttgata atatcaacct gattgactgg ataaatgtaa ttcatgaatg ctccctatta
      301 tctgaagttt tgaataattg cagcatcaac atccttaatc actttcactg tattggattg
      361 cgcgttccta tataatgtat attcagataa tttactttgg tattcagcca gcaactgtgg
      421 attcgaagga tttttagcta atttatctag tgccaatgtt agttcacctt gtaatgtttg
      481 agttccatca tcaaaagttt cagataatga actcaatgtc caatcatcat tcggtactgt
      541 aacactcatt ttatcctcac ttatttttat ctaaaaaaaa ccagtgttta tcattcaaca
      601 ttacataact gtctttgctg ttaagatatg atttaccttt aaaatcatca tctatcaaaa
      661 gtacagaaaa ctcaatatac tgatctccat atatattttt atgttcagaa atcagtctat
      721 taatgagctc aagcttttca tctgtcagtt cttctctgac agaatatgta actttattat
      781 tttc
//
LOCUS       DAZPEU010000488          782 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPEU010000488 DAZPEU010000000
VERSION     DAZPEU010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.498"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..666)
                     /locus_tag="ACHU2L_004550"
     CDS             complement(<1..666)
                     /locus_tag="ACHU2L_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFR1359064.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..>782)
                     /locus_tag="ACHU2L_004551"
     CDS             complement(656..>782)
                     /locus_tag="ACHU2L_004551"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR1359065.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          177 g          151 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 tt
//
LOCUS       DAZPEU010000489          779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPEU010000489 DAZPEU010000000
VERSION     DAZPEU010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.499"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHU2L_004552"
     CDS             complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHU2L_004552"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR1359066.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          189 a          207 c          174 g          209 t
ORIGIN      
        1 ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt gcatcgacaa
       61 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
      121 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      181 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      241 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      301 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      361 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      421 aatcaccaca tgccgcgcgg tatatccctg acgaccaatg acagccagca agtcttcact
      481 gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga aaagggagac
      541 ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac agccaaccgg
      601 gcatccctgt aaacgaataa aaatggcggg aacgccggta aagtaaccct caccttgcag
      661 ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt ggataattgt
      721 taattattgc agatcctgcc gcaacaatca tgtcttatta acattctgtt acatgcaga
//
LOCUS       DAZPEU010000490          779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPEU010000490 DAZPEU010000000
VERSION     DAZPEU010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.500"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..456)
                     /gene="yhaB"
                     /locus_tag="ACHU2L_004553"
                     /pseudo
     CDS             complement(1..456)
                     /gene="yhaB"
                     /locus_tag="ACHU2L_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            complement(712..>779)
                     /locus_tag="ACHU2L_004554"
     CDS             complement(712..>779)
                     /locus_tag="ACHU2L_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR1359067.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          229 a          153 c          152 g          245 t
ORIGIN      
        1 ctagggggtg ttaagatggc ttctttttgg gtgaactttg cgcaatactt agaaatcgtc
       61 tccttaatac attccagctc ttttttattg acggctgagc aagtacattg ttcaccaaaa
      121 ggtgcaaaag tgcctacatc taatgtttgt atgatataat catcatgttc tcctgtagaa
      181 atatctatga tgaatggact catattttcc ggttggattc ggaatcatga cagaaggaac
      241 atcgccttga tagaaattca atgcagtaat tgcagataat actctccgga gttcattact
      301 tatactgtaa attttattaa cgtcattggt ggagacaatt tctgcaaact ttctttttac
      361 cgtccaataa tcaatatttc tattgtgatt gttaaccact gcgtgcattg gcgggattga
      421 agggtgaaaa atatgtgcaa ttgggaagcc tttcattgat gcctgccttg tcgtcatgac
      481 aaacaataat agatgcttat tttcctcgca cgacacttac gcactatcaa aaaacgctca
      541 tttacccggc gttacagtaa acgaagcaac attctgtgtc ctacgctgat gtaaagcaca
      601 gtgaaaatcc cccttgttga cttcgtctga ccttatcgcg gcgaccagca attattcgtt
      661 ttgtgatcct caacacaaaa tgctattcgt cgcctatgag gggagaagaa tttatggaaa
      721 acgattgcat tcacaaaatg acgtcctgtc agaagagaaa aagttgacgt taatcaatt
//
LOCUS       DAZPEU010000491          778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPEU010000491 DAZPEU010000000
VERSION     DAZPEU010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.501"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(32..736)
                     /locus_tag="ACHU2L_004555"
     CDS             complement(32..736)
                     /locus_tag="ACHU2L_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR1359068.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          178 a          208 c          200 g          192 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
      661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
      721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcacca
//
LOCUS       DAZPEU010000492          773 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPEU010000492 DAZPEU010000000
VERSION     DAZPEU010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.502"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..641
                     /locus_tag="ACHU2L_004556"
     CDS             <1..641
                     /locus_tag="ACHU2L_004556"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFR1359069.1"
                     /translation="TIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSI
                     ASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVW
                     IGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRI
                     KWWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
BASE COUNT          245 a          114 c          162 g          252 t
ORIGIN      
        1 taacaattga agataatgtt attgctgaag aatattcaac aatgcctgtg aaaaatttct
       61 ccagtgtcgg cgctttttca tttccgacct gccatttttc agggaatata cgtataggtc
      121 gattttgtag tattgcatca aatgtaaaaa taatgggtgg aaatcatcct ttgaatcgtt
      181 ttactactca catgatgact tataacggtg aatttgataa gtttgctatg agtgaatttg
      241 aacgtagctg gacattgaaa ccattcatta ccaaacctga aaatccgatt ataggaaatg
      301 atgtctggat aggaaatgat gttgtattga aaggtggcat cgcaataggt gatggtgctg
      361 ttattgctgc caattccgtt gttaccaaag atgtgccccc atatgccata gttgcaggag
      421 ttcctgcaaa aataatcaga tttaggtttg attcaaatgt tattgatgaa ctgttgagaa
      481 taaaatggtg gaattataac tactcagatc ttccggataa taataagtgt gatgatataa
      541 attattttgt tgaagagatg aatcgattga tatcaaatgg aaatattcaa gagagggact
      601 ataaaaaatt caatctttct gaggtattca gaggcctata attatctgtt ttgcatgata
      661 aatgggcaag ataaccaggg cggggtttgc aatttcaatg gggcagcgtc atcaaggagg
      721 ttgcaaacat cctgccatgc atatacttta cgccatcttt ttatatttac gtt
//
LOCUS       DAZPEU010000493          772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPEU010000493 DAZPEU010000000
VERSION     DAZPEU010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.503"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>772
                     /gene="spa32"
                     /locus_tag="ACHU2L_004557"
     CDS             <1..>772
                     /gene="spa32"
                     /locus_tag="ACHU2L_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR1359070.1"
                     /translation="KLSSIDNIDSLVLKKKRKVEIPEYSLIASNYFTIDKHFEHKHDK
                     GEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQDIKAREKINIGDMRGIFSYN
                     KSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHSLSIDKNIADIISLLNGSVAK
                     SFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSEMTYHFKQWGAGHSVSISVES
                     GSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKGN"
BASE COUNT          292 a          105 c          143 g          232 t
ORIGIN      
        1 aaactatctt caatagataa tattgatagt ctcgttttga agaagaagag gaaggttgaa
       61 attcctgagt actctttaat tgcatctaac tattttacta ttgataagca ctttgaacat
      121 aagcatgata aaggagaaat ttatagtggc attaaaaatg cgttcgaact tagaaacgaa
      181 cgagcgacat attctgatat tccagaatca atggccatta aagaaaatat tttgatacca
      241 gatcaagata tcaaagcaag ggaaaaaata aatatcggcg atatgagggg gatcttttca
      301 tataataaga gtggaaatgc agacaagaac ttcgaaagaa gtcatacttc ttctgtaaac
      361 cctgataatc tgctagaatc tgataataga aatggtcaaa ttggtttaaa aaatcatagc
      421 ttgtctattg ataagaatat tgctgacatc atttctttac taaatggaag tgttgctaaa
      481 tcatttgagc tgcctgtaat gaataaaaat actgcagaca taaccccatc catgtcattg
      541 caagaaaaat caatagttga aaatgataaa aatgtttttc aaaaaaatag tgaaatgact
      601 taccacttta aacagtgggg ggctggacat tctgttagta tttcagtgga gtctggttct
      661 tttgttctaa aaccgtcaga tcaatttgta ggaaataaac ttgacttaat tttgaaacaa
      721 gatgctgagg gtaattacag atttgatagc agtcaacata ataaggggaa ta
//
LOCUS       DAZPEU010000494          772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPEU010000494 DAZPEU010000000
VERSION     DAZPEU010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.504"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..243
                     /locus_tag="ACHU2L_004558"
     CDS             <1..243
                     /locus_tag="ACHU2L_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1359071.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            358..>772
                     /locus_tag="ACHU2L_004559"
     CDS             358..>772
                     /locus_tag="ACHU2L_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR1359072.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          206 a          198 c          201 g          167 t
ORIGIN      
        1 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
       61 tcggatggac gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg
      121 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
      181 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      241 taagggacac agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc
      301 ggattgtgcg atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg
      361 ccgatcatag caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa
      421 acacacgata aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac
      481 cgtgtcagcg acgttgccag aacgctctgc tgcgcccgtt cctctgttgg acgctggatt
      541 aactggttca cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt
      601 cgctggccgt ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc
      661 gactttggct accagcgttc acgctggagt acagaactgc tggcaataaa aatcaatgag
      721 ataaccggct gccagttaaa tgccggaacc gttcgccgct ggttgccgtc tg
//
LOCUS       DAZPEU010000495          768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPEU010000495 DAZPEU010000000
VERSION     DAZPEU010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.505"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          233 a          155 c          157 g          223 t
ORIGIN      
        1 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
       61 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
      121 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
      181 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
      241 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
      301 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
      361 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
      421 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
      481 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
      541 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
      601 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
      661 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
      721 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DAZPEU010000496          761 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPEU010000496 DAZPEU010000000
VERSION     DAZPEU010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.506"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>761)
                     /gene="ipaH7.8"
                     /locus_tag="ACHU2L_004560"
     CDS             complement(<1..>761)
                     /gene="ipaH7.8"
                     /locus_tag="ACHU2L_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFR1359073.1"
                     /translation="SVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEERGI
                     AFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKELNA
                     DNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLKFL
                     HVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPESF
                     SQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
BASE COUNT          238 a          112 c          169 g          242 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag a
//
LOCUS       DAZPEU010000497          759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPEU010000497 DAZPEU010000000
VERSION     DAZPEU010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.507"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>759
                     /locus_tag="ACHU2L_004561"
     CDS             <1..>759
                     /locus_tag="ACHU2L_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1359074.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAZPEU010000498          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPEU010000498 DAZPEU010000000
VERSION     DAZPEU010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.508"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>743)
                     /locus_tag="ACHU2L_004562"
     CDS             complement(<1..>743)
                     /locus_tag="ACHU2L_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1359075.1"
                     /translation="ENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYK
                     QCGVINPQNANTAYFGDTDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSA
                     YIHKNNKLLYNLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYIL
                     TLLNSMRNMEGNKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLD
                     VCNYNSETKVLTSHHGIIRNEENQCYCLGA"
BASE COUNT          242 a          141 c          110 g          250 t
ORIGIN      
        1 gcaccgagac aataacattg attttcttca tttcttatta ttccgtgatg actggttaaa
       61 actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt atacgtatca
      121 ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa attgatttca
      181 tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt accctccata
      241 tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta
      301 tcgcctgtat gatccccaag gaagatacaa ggggttatcg catcgtcctt taaaaaaaca
      361 tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta gagcaattta
      421 ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa ttcacataat
      481 aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga ataaagtaca
      541 gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc
      601 acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata gctatcaaac
      661 ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt aaaattaccg
      721 ctaagaagag taaagaaatt ttc
//
LOCUS       DAZPEU010000499          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPEU010000499 DAZPEU010000000
VERSION     DAZPEU010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.509"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            3..>743
                     /locus_tag="ACHU2L_004563"
     CDS             3..>743
                     /locus_tag="ACHU2L_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1359076.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLF"
BASE COUNT          227 a          139 c          175 g          202 t
ORIGIN      
        1 taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg cttgtcgttt ttatcaagcg
       61 catttcccac ctatgctgcg gatgatgtca aagtgactca aaacacttca gtttttaaac
      121 tgaagcttag tgcaacgcga ttaatctatg aacaagggtc ggatggcgca gcgataactg
      181 tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa agtctttgaa gaagataaag
      241 taactgcagc gccctttatt gtcactccgc cactgttcag gcttaatcca ggccagcaaa
      301 ataaaatgtg ggttgtgcag actgcgccag ctttacaaga cagggaaaaa ttacactggc
      361 tatgcgccac cggcattacg cctaaaaaga gtgatgtttg ggccgagaaa gataaagaaa
      421 aaattgctga aaatgtgaac ctgaacatcc aggttaacgt acatacctgc ataaaactta
      481 ttaccaggcc atctggtatt gaaggcaaga tggttaatgc gagtgagaag ttatcgtgga
      541 aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc gttctacatc aacctttctt
      601 ctttacaggt tggcggtagc aagattgaga aactagatta tattgcgccg ttttcgcaac
      661 atgagtttgt tatgccaaaa actagcggcc aggatattaa atggtctgtg attactgatg
      721 aaggtggtga gagtcgtttg ttt
//
LOCUS       DAZPEU010000500          740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPEU010000500 DAZPEU010000000
VERSION     DAZPEU010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.510"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>740
                     /gene="gtrII"
                     /locus_tag="ACHU2L_004564"
     CDS             <1..>740
                     /gene="gtrII"
                     /locus_tag="ACHU2L_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR1359077.1"
                     /translation="IYCYWGWLYDGTLNIDGEFTNNFYQTITLGRWFHTFLRHYFLPE
                     PFSLYITPLIALSFIIISAFIICRSLKLESYELLIGMLVFITFPQISYQLEFLNQADT
                     VGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMAIYQTFVTYIIAFVIGLQINSII
                     RNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKHYFSLESNEYISNYIQNASDIKW
                     LVKSAIDNIYNFYNNPPAGLNLYKWLLI"
BASE COUNT          223 a          135 c          108 g          274 t
ORIGIN      
        1 caatttattg ttattggggg tggttgtacg atggaacact taatattgat ggcgagttta
       61 caaataattt ttatcaaaca atcacgcttg ggcggtggtt tcacactttt ttgcgacatt
      121 actttctccc tgagcctttt tcactttata taacaccatt aatagcctta tcttttatca
      181 tcatttcagc atttataatc tgcagatcgc taaagctaga atcttatgaa ttattgatag
      241 gtatgttagt atttattacc ttccctcaga tctcctatca attagagttt cttaaccaag
      301 ctgatactgt gggaattgct tttctactgg cagcgatatc agcaattatt tttcactcgc
      361 aaaaaaatag gattgtgata ttttctggta tagtactgtc cattctttca atggcaatct
      421 accaaacatt cgtaacatat attattgcat tcgtcattgg gttgcagata aattcgataa
      481 tacgaaatga gaaaaatatt cgtgaatctt tttatagttc atgtttatct ctatccctca
      541 tagctttatc taccttaatt tacctgctat taaccaaagc tatcaagcat tatttttcgc
      601 ttgaatcgaa cgagtacatc tcaaattata tacaaaatgc aagcgatatt aaatggcttg
      661 ttaaatcagc catagataat atatataact tctataacaa tcctcccgct ggtttaaacc
      721 tatacaagtg gttactgatt
//
LOCUS       DAZPEU010000501          740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPEU010000501 DAZPEU010000000
VERSION     DAZPEU010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.511"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..573)
                     /gene="fimE"
                     /locus_tag="ACHU2L_004565"
     CDS             complement(<1..573)
                     /gene="fimE"
                     /locus_tag="ACHU2L_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR1359078.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINE"
BASE COUNT          228 a          183 c          147 g          182 t
ORIGIN      
        1 ttcgtttatg agattatttc tttcccataa tccggcaaaa cgagcagcat tactggcggt
       61 ataacgcaca gtatggcgaa tatttcgatg cccgagataa tcctgaatta aacgggtatc
      121 tgcaccacgc tccgccagtt cataaccgca agcatgcctt aacatatgag gatgagtctg
      181 cgttacggtt ccagcttcaa taccggcatc gcgaataatg cgataggcct gctggcgaga
      241 aagccgactc ccgcggcgag aaataaatat agcgtcggtc cggtcagcgc ctttccagtt
      301 agcacgttcc tgggtccagc gttccacggc ttcacgctca tcaaaacgta acgggtgaac
      361 ggtagaaaat ccgttcttca gtcggcgaat atttattcga ccttcattaa ggtcaaggtc
      421 ctgataatgc agatcaagca gttcactaat acgcatccca tgccgatatg ccaacagaat
      481 aagacaataa tctctggctc ccgttgcccc gtaacaaacc gcctgcatca tggcctgaac
      541 ttctttaccg gtaagataac gacgtttact cacaatagta gtactcctaa ctgagatata
      601 tttgaatgaa tacctatagg aaacctcaat cggtcaaaat tagcccaata gataaaaaga
      661 taaccacaca cgaagttatg tggctatcag tcaattacat taaacatcat acaaaaataa
      721 aatatcactg atgtgacaac
//
LOCUS       DAZPEU010000502          704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPEU010000502 DAZPEU010000000
VERSION     DAZPEU010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.512"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..443)
                     /locus_tag="ACHU2L_004566"
     CDS             complement(<1..443)
                     /locus_tag="ACHU2L_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR1359079.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAZPEU010000503          697 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPEU010000503 DAZPEU010000000
VERSION     DAZPEU010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.513"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..697
                     /gene="virF"
                     /locus_tag="ACHU2L_004567"
     CDS             <1..697
                     /gene="virF"
                     /locus_tag="ACHU2L_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFR1359080.1"
                     /translation="SSGNETLTIDEGQIAFIERNIQINVSIKKSDSINPFEIISLDRN
                     LLLSIIRIMEPIYSFQHSYSEEKRGLNKKIFLLSEEEVSIDLFKSIKEMPFGKRKIYS
                     LACLLSAVSDEEALYTSISIASSLSFSDQIRKIVEKNIEKRWRLSDISNNLNLSEIAV
                     RKRLESEKLTFQQILLDIRMHHAAKLLLNSQSYINDVSRLIGISSPSYFIRKFNEYYG
                     ITPKKFYLYHKKF"
BASE COUNT          254 a          101 c          112 g          230 t
ORIGIN      
        1 tagctcaggc aatgaaactt tgactatcga tgaagggcaa attgctttta tagagcgaaa
       61 tatacaaata aacgtctcca taaaaaaatc tgatagcatt aatccatttg agattataag
      121 ccttgacaga aatttattat taagcattat tagaataatg gaaccaattt attcatttca
      181 acactcctat tctgaggaga aaagggggtt aaacaaaaaa atattcctcc tctctgagga
      241 ggaggtttct atcgatttgt tcaaatctat aaaagagatg cctttcggca aaagaaagat
      301 ctatagttta gcttgccttt tatcagctgt ttctgatgag gaagctttat atacttcgat
      361 atcgatagct tcttctctta gtttttctga tcagataagg aagattgttg aaaaaaacat
      421 cgagaagaga tggcgtcttt ctgatatttc aaataacttg aatttatcag aaatagctgt
      481 tagaaaacga ttggagagtg aaaaattaac atttcaacaa atccttcttg atattcgcat
      541 gcatcatgca gcaaagcttt tattgaatag tcaaagctat attaatgatg tatcaagact
      601 tatcggaata tcaagcccat cttattttat aaggaaattt aatgaatatt atggtataac
      661 tccaaagaaa ttttacttat atcataaaaa attttaa
//
LOCUS       DAZPEU010000504          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPEU010000504 DAZPEU010000000
VERSION     DAZPEU010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.514"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            32..493
                     /locus_tag="ACHU2L_004568"
     CDS             32..493
                     /locus_tag="ACHU2L_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR1359081.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>696)
                     /locus_tag="ACHU2L_004569"
     CDS             complement(534..>696)
                     /locus_tag="ACHU2L_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1359082.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          204 a          152 c          160 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatg
//
LOCUS       DAZPEU010000505          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPEU010000505 DAZPEU010000000
VERSION     DAZPEU010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.515"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..673
                     /locus_tag="ACHU2L_004570"
     rRNA            <1..673
                     /locus_tag="ACHU2L_004570"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAZPEU010000506          689 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPEU010000506 DAZPEU010000000
VERSION     DAZPEU010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.516"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(193..372)
                     /locus_tag="ACHU2L_004571"
                     /pseudo
     CDS             complement(193..372)
                     /locus_tag="ACHU2L_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            466..>689
                     /locus_tag="ACHU2L_004572"
     CDS             466..>689
                     /locus_tag="ACHU2L_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1359083.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          181 a          140 c          184 g          184 t
ORIGIN      
        1 gttgggttga agagtagctg gtagatgtta ttgtgccagc aggggcaggg gaatgtgccg
       61 tatcgatatg ctttggcaat tgcgtgtgcc gcagatggag atgaaatgcc aataaaaaag
      121 cctgagttcg atgagggacg aactcaggct ggtgttatgt tttacagtaa cacgcctcgg
      181 cgcgttggct gattagaact ggtagacgat accaactgcc agttggtcgt cggtgttcag
      241 tccatgagcg agaacataat cactcttatc aagcaggttg atctgataag cggtatatat
      301 gttcatattc ttattgaagt aataccaggt accgatttca atatagttca gacgatcaga
      361 atctttgtaa cctgtagact ggccccctga atctccagac aaccaatatc acttatttaa
      421 gtgatagtct taatactagt ttttagacta gtcattggag agcagatgat tgatgtctta
      481 ggaccggaga aacgcagacg gcgtactaca caggaaaaga tcgctattgt tcagcagagt
      541 tttgaaccgg gaatgacggt ctcccttgtt gcccggcaac acggtgtggc agccagccag
      601 ttatttctct ggcgcaagca ataccaggag ggaagtctta ctgctgtggc cgcaggagaa
      661 caggtcgttc ctgcctctga acttgctgc
//
LOCUS       DAZPEU010000507          685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPEU010000507 DAZPEU010000000
VERSION     DAZPEU010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.517"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            52..685
                     /locus_tag="ACHU2L_004573"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACHU2L_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZPEU010000508          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZPEU010000508 DAZPEU010000000
VERSION     DAZPEU010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.518"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>684
                     /gene="ipaH3"
                     /locus_tag="ACHU2L_004574"
     CDS             <1..>684
                     /gene="ipaH3"
                     /locus_tag="ACHU2L_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR1359084.1"
                     /translation="GTYADYFSAWDKWEKQALPGENRNEAVSLLKECLINQFSELQLN
                     RLNLSSLPDNLPPQITVLEITQNALISLPELPASLEYLDACDNRLSTLPELPASLKHL
                     DVDNNQLTMLPELPALLEYINADNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLE
                     ALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENRITHIPENILSLDPTCTIILEDNP
                     LSSRIRESLS"
BASE COUNT          202 a          181 c          122 g          179 t
ORIGIN      
        1 cggtacatat gctgattact tttcagcatg ggataaatgg gaaaaacaag cgctccccgg
       61 tgaaaatcgg aatgaagcgg tctccctact taaagaatgt ctcatcaatc agttcagtga
      121 gcttcaactg aatcgtttaa atctgtcctc gctacctgac aacttaccac ctcaaatcac
      181 tgttctggaa attactcaga atgccctaat atcattacca gaattgccag catcgctgga
      241 ataccttgac gcctgtgaca atcgcctgtc aacacttcct gaattacccg catctctgaa
      301 acatcttgat gtagataaca accaactaac catgcttcct gaattgcctg cattgctgga
      361 atatattaat gcagataaca atcagctaac catgcttcct gaattaccta catcgctgga
      421 agtgctctca gtaagaaata accagctgac atttctccct gagttacctg aatcactgga
      481 agcgctcgat gtaagtacta atcttctgga aagcctacca gccgtacctg taagaaatca
      541 tcactcagag gaaaccgaga tatttttccg gtgccgcgag aatcgcatca cacacattcc
      601 ggaaaatata cttagccttg atccgacctg cactatcatc ctcgaagaca atcctctgtc
      661 ctcacggatc agggagtctc tgtc
//
LOCUS       DAZPEU010000509          669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZPEU010000509 DAZPEU010000000
VERSION     DAZPEU010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.519"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..417)
                     /locus_tag="ACHU2L_004575"
     CDS             complement(<1..417)
                     /locus_tag="ACHU2L_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR1359085.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQ"
     gene            complement(427..>669)
                     /locus_tag="ACHU2L_004576"
     CDS             complement(427..>669)
                     /locus_tag="ACHU2L_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR1359086.1"
                     /translation="GALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSLQTAISG
                     GTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          161 a          194 c          157 g          157 t
ORIGIN      
        1 ttgcattgct tctggagacg atgacgcaag gcgtgcgcca gccgctaagg tgccgataga
       61 attacgctgc tcttcatcaa tgaatcccat gcctttacga acggattcaa tctggtctgg
      121 atattgagta gccaactgac gcaaagcacc gcgatcacca gacgcataag cattagcgta
      181 cgcctgctga aattctttct gccgctgagc ctgcttttcc tgctgaaaaa cacccgcaat
      241 acctgaaaga ccttgcaaag cagtcagccc aacattgtta gcgcctgaac gctcaatatc
      301 attgttctgc ctgataagct gaagcgtatt gccgatgtca tttacgctcg gagcgtttga
      361 gttgacgccg ccgataccag ccaacaatcc gccgtttgtt ccttgccaag tagccatgat
      421 tcccccttaa aacaacgagc caagcaatcc gataccagca ccaataccag cgccccaagg
      481 cgttgatgtt cccaaaaggc tggcaagacc tgcaccggca atcgcaccag acgtgccacc
      541 gctaattgca gtctgaagac ttgatggttt attggcatta gcagcggcaa gagctgcgct
      601 ttgctgtgca atgctgctca tgttgttggc gtacgtctgc ccggcgtttg cctgaccttg
      661 cagcgcacc
//
LOCUS       DAZPEU010000510          668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZPEU010000510 DAZPEU010000000
VERSION     DAZPEU010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.520"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>668
                     /locus_tag="ACHU2L_004577"
     CDS             <1..>668
                     /locus_tag="ACHU2L_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR1359087.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAZPEU010000511          659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZPEU010000511 DAZPEU010000000
VERSION     DAZPEU010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.521"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            103..>659
                     /locus_tag="ACHU2L_004578"
     CDS             103..>659
                     /locus_tag="ACHU2L_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1359088.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          110 g          192 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcat
//
LOCUS       DAZPEU010000512          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZPEU010000512 DAZPEU010000000
VERSION     DAZPEU010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.522"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>656
                     /locus_tag="ACHU2L_004579"
     CDS             <1..>656
                     /locus_tag="ACHU2L_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359089.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAZPEU010000513          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZPEU010000513 DAZPEU010000000
VERSION     DAZPEU010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.523"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(151..>656)
                     /locus_tag="ACHU2L_004580"
     CDS             complement(151..>656)
                     /locus_tag="ACHU2L_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1359090.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAZPEU010000514          644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPEU010000514 DAZPEU010000000
VERSION     DAZPEU010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.524"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..204
                     /locus_tag="ACHU2L_004581"
                     /pseudo
     CDS             1..204
                     /locus_tag="ACHU2L_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(270..>644)
                     /gene="istB"
                     /locus_tag="ACHU2L_004582"
     CDS             complement(270..>644)
                     /gene="istB"
                     /locus_tag="ACHU2L_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR1359091.1"
                     /translation="DAYADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFS
                     QEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHIVQI
                     KGESYRLRQKRKAGVIAEANPE"
BASE COUNT          162 a          150 c          160 g          172 t
ORIGIN      
        1 atgacaggcg aaaatcgtgg ccggggaagg ccgcgaaaga cagaaagtac ctatgcagat
       61 actcgtaatg atctgatccg cagtggcctt gaactgctga cgcagaatgg ttttcttgcg
      121 acgggagtcg atgccattgt taaaaatgcc aatgtcccta aaggctcatt ttattactac
      181 cttaagagta aagaggatta tgctgtcaac gacggatgaa aagtgatcca cttatatctc
      241 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg
      301 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgat atgtgagtgg
      361 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
      421 cactgcccga acggcagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
      481 atgacctgga agaacagctt tgcttcttcc tgactgaacg gcagatagcc tatttcatca
      541 atgatgagca ggcggggggc cattactcca cgctgaagcg tcgttttata acggccctga
      601 cgttgtgccg tagataactg aagtaacaga tctgcgtaag cgtc
//
LOCUS       DAZPEU010000515          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPEU010000515 DAZPEU010000000
VERSION     DAZPEU010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.525"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHU2L_004583"
     CDS             complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHU2L_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HFR1359092.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHU2L_004584"
     CDS             complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHU2L_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR1359093.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          166 a          146 c          155 g          174 t
ORIGIN      
        1 tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt aacttcagga
       61 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc
      121 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
      181 ttctgtaata caaatgatgt attaatactg atgagcggtc gcgttgttac caaagccaac
      241 ctgagtcacg ttgacggagg agttcgatgc agtctggtca actgcagcac cgttgccacc
      301 accgaactgt ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct
      361 gttaccgaag ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc
      421 tgcaccatta ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg
      481 cagagcaagt gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc
      541 gctattatta ctgaggtgta ctggcaatag cggacactac catttgttct tttttaagca
      601 gccatctgat gatatttttc cctgaaggct gccggggaga t
//
LOCUS       DAZPEU010000516          637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZPEU010000516 DAZPEU010000000
VERSION     DAZPEU010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.526"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            20..493
                     /locus_tag="ACHU2L_004585"
     CDS             20..493
                     /locus_tag="ACHU2L_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1359094.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          129 g          185 t
ORIGIN      
        1 gggagatgaa ccctatgcaa tgtcctggtt aatatctcag tgtgctcatc agtgcactga
       61 taataaaaag acggagacag atgccattta cgataaagtt cggagttctt atttgctttc
      121 atgtattctg aaaaaaaaca aaaatgttgg gcttattctt catgcaccat catttgtttc
      181 tgtgtctgaa aaaatcgcca gaattgtgat ggcaaattac tcccgtaact ggagtaattc
      241 cgagttggcc agtgcggttt taatgagtga atcttcttta aaaagaagaa tgtacaaaga
      301 ggtcggttct ataagtacat ttgtacataa aataaagctt acagaagcta tcaggaaatt
      361 acgaaggaca aatactccca tttctgtcat atcatctgag ttaggttaca gttcaccatc
      421 gtatttttct aaagtcttct ttaaatattt aaagacatac cctcaaaaca taaggaagaa
      481 aaatggtcgg taatgtcacc aattaggagg acttgcagag ccgctttttt acgatactga
      541 gatcaaacag acataatcta tgcggagatt tacagcaggg catggttcac aaatctgaca
      601 gcgatgaatt atcggcactg cgggcagaaa atgctcg
//
LOCUS       DAZPEU010000517          632 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPEU010000517 DAZPEU010000000
VERSION     DAZPEU010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.527"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(36..>632)
                     /gene="spa40"
                     /locus_tag="ACHU2L_004586"
                     /gene_synonym="spaS"
     CDS             complement(36..>632)
                     /gene="spa40"
                     /locus_tag="ACHU2L_004586"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFR1359095.1"
                     /translation="ALTTYFFWINDRKIIFSQVFSSVDGLYLIWGRLFKDIILFFLAF
                     SVLVIILDFVIEFILYMKDMMMDKQEIKREYIEQEGHFETKSRRRELHIEILSEQTKS
                     DIRNSKLVVMNPTHIAIGIYFNPEIAPAPFISLIETNQCALAVRKYANEVGIPTVRDV
                     KLARKLYKTHTKYSFVDFEHLDEVLRLIVWLEQVENTH"
BASE COUNT          222 a          128 c           82 g          200 t
ORIGIN      
        1 aaattaactc ccatataaca catctccttt acttattaat gagtgttttc aacctgctca
       61 agccaaacaa taagacgtag gacttcatcc aagtgttcaa aatcaacaaa actatatttt
      121 gtatgtgttt tatatagttt tctagctaat ttcacatcac gcacagtcgg tataccaact
      181 tcatttgcat attttctgac agccaaggca cactggttag tttcaatgag agaaataaaa
      241 ggtgcaggcg ctatttctgg attaaaataa ataccaattg caatatgagt cgggttcatt
      301 accactaatt ttgaattacg tatatcagat ttagtctgct ctgaaagaat ctcgatatgc
      361 aactcacgcc ttctcgactt tgtctcaaag tgtccctctt gctctatata ttctcttttt
      421 atctcctgtt tatccatcat catatctttc atgtataaaa tgaactcaat cacaaagtca
      481 agaataataa caagaacaga aaatgccaag aaaaataata ttatatcctt aaacagcctc
      541 ccccaaataa gatataagcc atcaacacta gaaaacacct gagaaaaaat tatttttcgg
      601 tcattaatcc aaaagaaata ggttgttaat gc
//
LOCUS       DAZPEU010000518          630 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZPEU010000518 DAZPEU010000000
VERSION     DAZPEU010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.528"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>630)
                     /gene="ipaH9.8"
                     /locus_tag="ACHU2L_004587"
     CDS             complement(<1..>630)
                     /gene="ipaH9.8"
                     /locus_tag="ACHU2L_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR1359096.1"
                     /translation="GTYADYFSAWDKWEKQALPGEERDEAVSRLKECLINNSDELRLD
                     RLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKLYSASNKLSELPVLPPALESL
                     QVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLRATRNFLTELPAFSEGNN
                     PVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSSHALQALQRLTSS"
BASE COUNT          175 a          109 c          154 g          192 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gcatgggatg ataatgggtt atcactaata
       61 tgtattgaac attcattcct cagattaaga atgctttccg ggatatgact tatctgattt
      121 ctatcaaaaa aatactctct gacaacggga ttatttccct cagaaaatgc tggtagctca
      181 gtgaggaaat tacgggtcgc tctgagattt ttaagagcct gtgggagcga tggtaaggag
      241 actatttcgt tatagctgat attcatagtc aataacgaat cgggtaaagc tggcaggttt
      301 tccagctcat tgtgttgtac ctgaagtgac tccagcgcag gaggtagcac gggcaattct
      361 gataatttat tgctggcgga atataatttt tttagcgtaa caggcagttc aggtaggtta
      421 gttaattgat tatatgatac attgagcagc gttatctgag ctggtaagtt gtcaggtagc
      481 gaggacagat ttaaacggtc cagtcgaagt tcatcggaat tattgataag acattcttta
      541 agtcgggaga cagcctcatc acgctcttca ccggggagcg cttgtttttc ccatttatcc
      601 catgctgaaa agtaatcagc atatgtaccg
//
LOCUS       DAZPEU010000519          623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZPEU010000519 DAZPEU010000000
VERSION     DAZPEU010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.529"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..>623
                     /gene="ospC1"
                     /locus_tag="ACHU2L_004588"
     CDS             1..>623
                     /gene="ospC1"
                     /locus_tag="ACHU2L_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR1359097.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGA"
BASE COUNT          239 a          110 c           90 g          184 t
ORIGIN      
        1 atgaatatat cagaaacact gaactcagca aatacccaat gcaatataga ttctatggat
       61 aacagattac atacattgtt tccaaaagtg acatcagtgc gaaacgctgc acaacaaact
      121 atgccagatg aaaaaaattt aaaagatagt gcaaatatta ttaaaagttt ctttaggaaa
      181 actatagcag cacagagtta tagtagaatg ttctctcaag gctctaactt taaatcttta
      241 aatatagcaa ttgatgcacc atcagacgct aaagcctcat ttaaggctat tgagcacctt
      301 gacagattat cgaagcatta tatatctgaa ataagggaaa aacttcatcc tctttctgca
      361 gaggaactca atttgctttc gctaattatt aattctgatt taatcttcag acatcaaagt
      421 aattctgatt tgtctgataa aattttaaac attaagtcat tcaataaaat tcagtctgaa
      481 ggaatatgca caaaacgaaa cacatacgct gatgatataa aaaaaatagc taatcatgac
      541 tttgtgtttt ttggcgttga aatctctaac catcagaaaa aacaccccct gaatacaaaa
      601 catcacactg ttgattttgg tgc
//
LOCUS       DAZPEU010000520          623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZPEU010000520 DAZPEU010000000
VERSION     DAZPEU010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.530"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /locus_tag="ACHU2L_004589"
     CDS             complement(<1..251)
                     /locus_tag="ACHU2L_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR1359098.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>623)
                     /locus_tag="ACHU2L_004590"
     CDS             complement(272..>623)
                     /locus_tag="ACHU2L_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1359099.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          141 a          145 c          141 g          196 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gta
//
LOCUS       DAZPEU010000521          616 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZPEU010000521 DAZPEU010000000
VERSION     DAZPEU010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.531"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(15..230)
                     /locus_tag="ACHU2L_004591"
     CDS             complement(15..230)
                     /locus_tag="ACHU2L_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1359100.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
     gene            complement(523..597)
                     /locus_tag="ACHU2L_004592"
     tRNA            complement(523..597)
                     /locus_tag="ACHU2L_004592"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(562..564),aa:Gly,seq:tcc)
BASE COUNT          190 a          152 c          136 g          138 t
ORIGIN      
        1 taatgatggc gacattattc acctctcgca gccttacgct tgtcttctct gattttgaag
       61 tacagatttg tcagataagt caggaagccc agaaccagac tccccagtac accaatcgca
      121 gcccactgtg acggactgac ctgatcaagc cactgtaaaa accagtagcc agcactgcct
      181 gcggaggtgc cgtaggcaat gcccgttgaa attttgtcca tggatttcat agcctcacct
      241 ccgcaaataa cggatggcgt agttttacac tgagaaatga aagggatttg aaaagaaaaa
      301 accgcaaaag cgggcgaaac gatatataca ataaggaaag cactctatcc aacaaaccac
      361 tcacagttaa tcggaataaa agcagagtgc ttatgaatga tcgcctgccc gaaggttagt
      421 atttctgcac agcaattttg caaaaaaaag cgatcattca taacttaaac gtctttcagt
      481 cactccggga tttcccatca tcgcagactg aaagactctg actggagcgg gcagcgggaa
      541 tcgaacccgc atcatcagct tggaaggctg aggtaatagc cattatacga tgcccgcata
      601 tggtgccgac taccgg
//
LOCUS       DAZPEU010000522          614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZPEU010000522 DAZPEU010000000
VERSION     DAZPEU010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.532"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..614
                     /locus_tag="ACHU2L_004593"
                     /pseudo
     CDS             1..614
                     /locus_tag="ACHU2L_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          203 a           85 c          111 g          215 t
ORIGIN      
        1 taatgatact ataagtgtaa aattctacag aaatgaaaaa atagaatgcg cttgtaattt
       61 tctgatggat aaagatgcgc aggggtatat cgacctgtct gattttgatt taacaaattg
      121 tcattttaaa ggtgacgtta tttctaaggt gtctttttta tcatcaaatc tacaacatgt
      181 aactttcgaa tgtaaagaaa ttgagaattg caattttact aaggcaacag ttgataatgt
      241 catatttaaa tgtcgacgtt tacacaatgt gatttttctc aaaacgagtg gcgaatgtgt
      301 tgattttagc caaaatattc ttgatacagt tgacttctcg cagagtcaac ttggtcatag
      361 taattttcgc gaatgtcaga ttagaaattc aaacttcgat aattgttatc tttacgcttc
      421 gcacttcacc agagcagagt ttctgtctgc caaagaaata tcatttatta aatcgaattt
      481 gacagctgtt atgtttgatc atgtgcgaat gtcgacaggg aatttttaag attgcattac
      541 agaacaattg gaattaacta ttgattattc agatatattt gggaatgaag atctcgatgg
      601 ttatatcaat aaca
//
LOCUS       DAZPEU010000523          611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZPEU010000523 DAZPEU010000000
VERSION     DAZPEU010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.533"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            164..>611
                     /locus_tag="ACHU2L_004594"
     CDS             164..>611
                     /locus_tag="ACHU2L_004594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359101.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIM"
BASE COUNT          198 a           78 c          141 g          194 t
ORIGIN      
        1 aacaaatatt gatggcaata tcattataca taaggatggt aggaggacgt atttattaaa
       61 tcaaaatact ggttcgatta cggctgtgat aagatggaag tatgagtagt ataaggataa
      121 taccgttaca gatgactgga ctatatggga aaaagctatt tttatgcgag cgatatattt
      181 atctgttcag caggcatgga atgggaaaat aacttattct gtttccggtg aatctgagtt
      241 cgctaaaaaa tttcagggta aagctttgcc ttttgatgta agaataattt ctgcatctca
      301 gaatgaagac tggttggtta ttgcaacaaa ggttttgcct ggtgctgatt taagaacata
      361 tgttgatttt aaaaacagta cggttcatgt tgattcagcg ggcctggaaa aagtagcgaa
      421 atgtattaac tgtaataata cgttgcaagt aaatatacct catgaagccg gacatgtact
      481 tggttactta gatgatgatt atgattcttc gtctccgtat gttggagata ttagtgggtt
      541 gatgaatgtg ggaatggagc tttgggaacg ataccttaaa aacgcaacta taacgttgaa
      601 tatcataatg c
//
LOCUS       DAZPEU010000524          606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZPEU010000524 DAZPEU010000000
VERSION     DAZPEU010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.534"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            19..>606
                     /gene="virA"
                     /locus_tag="ACHU2L_004595"
     CDS             19..>606
                     /gene="virA"
                     /locus_tag="ACHU2L_004595"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR1359102.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSN"
BASE COUNT          230 a          119 c           93 g          164 t
ORIGIN      
        1 tacatcagga gaaatcaaat gcagacatca aacataacta accatgaaag aaatgactca
       61 tcatggatgt caactgtcaa atcaactaca gaagtaagtt ggaataaatt gagtttttgt
      121 gacattttac tgaaaataat aactttcggc atatattctc cacacgaaac tctagccgaa
      181 aaacatagcg aaaaaaaact aatggatagt ttctcaccat cactatcaca agataaaatg
      241 gacggagagt ttgcccacgc aaacatagat ggcatttcaa tacggctatg ccttaacaaa
      301 ggcatatgtt cggtttttta tcttgatgga gataaaatac aatccactca actttcatca
      361 aaagaatata ataacttgtt aagttcactc ccccccaaac aattcaactt aggaaaagta
      421 catactatta cagctccggt atcagggaat tttaaaactc acaagcctgc accagaagtt
      481 attgaaacag ccattaattg ctgtacatcg ataataccaa acgatgatta cttccacgtt
      541 aaagacactg attttaatag tgtttggcat gatatttaca gagatattcg agcatctgac
      601 agcaat
//
LOCUS       DAZPEU010000525          590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZPEU010000525 DAZPEU010000000
VERSION     DAZPEU010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.535"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..558
                     /locus_tag="ACHU2L_004596"
     CDS             <1..558
                     /locus_tag="ACHU2L_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR1359103.1"
                     /translation="KIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAII
                     EVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQE
                     DQLGALGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYS
                     FTLAELVTKGHLRPLKEASEAENVA"
BASE COUNT          159 a          146 c          154 g          131 t
ORIGIN      
        1 aaaatagtcc ttcagtggga cgaaatgatc cggaccgctg gctccctgaa gctgggcaaa
       61 gtacaggctt cagtgctggt ccgttcattg ctgaaaagtg aacgtccttc cggactgact
      121 caggcaatca ttgaagtggg gcgcatcaac aaaacgctgt atctgcttaa ttatattgat
      181 gatgaagatt accgccggcg cattctgacc cagcttaatc ggggagaaag tcgccatgcc
      241 gttgccagag ccatctgtca cggtcaaaaa ggtgagataa gaaaacgata taccgacggt
      301 caggaagatc aactgggcgc actggggctg gtcactaacg ccgtcgtgtt atggaacact
      361 atttatatgc aggcagccct ggatcatctc cgggcgcagg gtgaaacact gaatgatgaa
      421 gatatcgcac gcctctcccc gctttgccac ggacatatca atatgctcgg ccattattcc
      481 ttcacgctgg cagaactggt gaccaaagga catctgagac cattaaaaga ggcgtcagag
      541 gcagaaaacg ttgcttaacg tgagttttcg ttccactgag cgtcagaccc
//
LOCUS       DAZPEU010000526          584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZPEU010000526 DAZPEU010000000
VERSION     DAZPEU010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.536"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>584)
                     /locus_tag="ACHU2L_004597"
     CDS             complement(<1..>584)
                     /locus_tag="ACHU2L_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1359104.1"
                     /translation="GSIKELLDYQFSTNEIAAVVAERDIEWWQNRASVLTTPQLASGY
                     FNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKY
                     NTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKE
                     DLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKY"
BASE COUNT          184 a          113 c           87 g          200 t
ORIGIN      
        1 atactttaaa taattaaaaa tgcctgccat ataatgcttt tgtttaaatt tatgttttgc
       61 acaataccga tactgattgc tatttacagg tttaagtaaa tcttctttac tccacggaga
      121 agctgctttg gcgatcaaaa aactacgtga aaccggataa tcagcccact catgccaggg
      181 ttttgtaggt cccacatagt gtataaacac ggtgtcatca ttgactggat tatcaacttt
      241 gtcttttaat tcatagttaa tactatatcg ggtattatat ttctccgaaa taaacttcac
      301 tttaccattc aataatacat tcagtacatc ttgatcaagg tgggtgattt tacttaccca
      361 atctgggtca cgcaacattt caatagcttt tgacgaaatg ttatttagat tccactcatc
      421 aatatttatc agtaaaaaac cagcattgaa atatccagaa gctaactgtg gtgtagttaa
      481 taccgaggct cgattttgcc accattcaat atctctttca gctacaactg cggcaatttc
      541 attagtagaa aattgataat ctaagagttc tttaatacta ccct
//
LOCUS       DAZPEU010000527          584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZPEU010000527 DAZPEU010000000
VERSION     DAZPEU010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.537"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(110..577)
                     /locus_tag="ACHU2L_004598"
     CDS             complement(110..577)
                     /locus_tag="ACHU2L_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR1359105.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
BASE COUNT          226 a          122 c          112 g          124 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgct
//
LOCUS       DAZPEU010000528          582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZPEU010000528 DAZPEU010000000
VERSION     DAZPEU010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.538"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          188 a           92 c          122 g          180 t
ORIGIN      
        1 tgatgcatta aattagtaga atatttattc ttttataaaa gcaccaatga attgtccaag
       61 taaacatgag ataaaatgtg tagaggataa ctgagtttgg tcactttaac tgtattagtc
      121 gcagtctgac tctcgacttt aaaaggatgg gaattacctg tactgatagt gagaaagctt
      181 tccattgaaa taagtcaaat aaaagttgat ggcttcagtg ctaaagtgag gtgcccgcta
      241 gccgtattca ctcgaatcgg aattcaatgt attgcttctg ccatttccat aatatttcac
      301 gggaaaaaca aaatctgaaa aactatactt aagtgttgta ttgagtcagt tgagatacaa
      361 atacatattt atcagcacac gttacgtcaa atacaggagg ttgtcatgtt ggtactgaaa
      421 ggcacgttgg ctattgcagc cgtgatgaca atttattgtc ttatcgttgt tcttatggaa
      481 cgcttttctg attgatttca tattggcgag agaacaagaa ggtgagtaga attgtcgcgg
      541 aatcctgaaa ctatcagcct caggtatgaa caccaaaagg ta
//
LOCUS       DAZPEU010000529          581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZPEU010000529 DAZPEU010000000
VERSION     DAZPEU010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.539"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(141..>581)
                     /locus_tag="ACHU2L_004599"
     CDS             complement(141..>581)
                     /locus_tag="ACHU2L_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1359106.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          147 a          163 c           99 g          172 t
ORIGIN      
        1 caccagcacg tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg
       61 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa
      121 gaattgaagc agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata
      181 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
      241 caggttctta aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg
      301 gtcatccccc gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc
      361 agcacctcac ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc
      421 cagctctttt gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc
      481 agacctgctt ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta
      541 gaggtcaaca tcaggttaaa catattcccc gtcttaccgc c
//
LOCUS       DAZPEU010000530          576 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZPEU010000530 DAZPEU010000000
VERSION     DAZPEU010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.540"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(29..397)
                     /locus_tag="ACHU2L_004600"
                     /pseudo
     CDS             complement(29..397)
                     /locus_tag="ACHU2L_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          164 a          106 c          132 g          174 t
ORIGIN      
        1 ttgtctggag attcaggggg ccagtctaat cagttgtcat gcactcggaa taaatcatac
       61 cgtgaatagt gctgtttttg attgtcagac tattggcgtt ggtattggta gaaataccgt
      121 cgtccagata gtaggtagaa atcacgccgt taacggtagc gttattaatg accgggtaga
      181 tatcgccgtt gtaatagcta tcagcggcat agttgttcca gcctacataa ccatcatagt
      241 aaacgtcatc agcataggtg gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag
      301 aaatatcgtt ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta
      361 attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt
      421 aataagttga tccatcaatt atcaatgcat agaaaacata ttatgtttcg cgctaacgat
      481 tatgctgcgt tacagacgaa tggttcaatt attctttccc ctaagtccga atgtggacat
      541 atataattta tggagaaaaa cacgcatcaa attgat
//
LOCUS       DAZPEU010000531          574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZPEU010000531 DAZPEU010000000
VERSION     DAZPEU010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.541"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            218..293
                     /locus_tag="ACHU2L_004601"
     tRNA            218..293
                     /locus_tag="ACHU2L_004601"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:251..253,aa:Ile,seq:cat)
BASE COUNT          156 a          106 c          143 g          169 t
ORIGIN      
        1 aagtatcatt gaatcgatga taactataga tagtgctaat tattaacatt tgcttgttga
       61 ctgcgagtat cgactttgcg aatggaggcg gtgtcgggtt ttatgtcgcg tgtaatcacg
      121 acccaaggtg aatgggaacg cataaaaatc tgttatttac atcataagat catcatgttt
      181 attgcgtggt ttataagtat tgttattgca tacttaaggc cctttagctc agtggttaga
      241 gcaggcgact cataatcgct tggtcgctgg ttcaagtcca gcaagggcca ccaaatcatc
      301 attcatgtat caggaagagt taagggtaca aggggtactc gatggcggtc tatatttcat
      361 gactaacgcc atttcaagga agaagtgcgg tgggtggttt cgaatgaggg ctggtgtttt
      421 ggtacgtggc acgccactgc aatgtattta ttcatcagta ctcattcaaa agtacctgga
      481 aaaaatgccc gctatgccaa gcgggcaaat tgatcctacc cacgtaatat ggacacaggc
      541 ctaagcgagg ttctggtttt caaattgttc cgga
//
LOCUS       DAZPEU010000532          568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZPEU010000532 DAZPEU010000000
VERSION     DAZPEU010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.542"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..455
                     /gene="ospC2"
                     /locus_tag="ACHU2L_004602"
     CDS             <1..455
                     /gene="ospC2"
                     /locus_tag="ACHU2L_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR1359107.1"
                     /translation="TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSL
                     GNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFIS
                     NGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          208 a           77 c          114 g          169 t
ORIGIN      
        1 gcacagataa cttcaaaaag ataagactca gagatatatc tttggaggat gccattaaag
       61 catccaatta tgaagaaatt aacaataagg tgactgataa aaaaatggct catcaagctc
      121 ttgcttattc tctaggcaat gcaaaatctg atatggcact ttatttacta tctaaattta
      181 attttacaaa acaagatatc gcagagatgg agaaaatgaa caacaatatg tattgtgagc
      241 tgtatgatgt tgagtattta cttagtgaag atagtgccaa ctataaagtg ctagaatatt
      301 ttattagtaa tggattggtt gatgtaaaca aaagatttca aaaagcaaat agtggggaca
      361 ctatgcttga caatgcaatg aaaagcaaag attcaaaaac aattgatttt ttattaaaaa
      421 atggagcggt atcaggcaaa cgatttggga ggtgacctaa aatatattta ggttgtgccc
      481 tgaatctgac gttgtcattt ttgttatatt gatcgatata ggggatggtg cagaccgttc
      541 ccctgcaaca acacattgca tgtggtga
//
LOCUS       DAZPEU010000533          567 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZPEU010000533 DAZPEU010000000
VERSION     DAZPEU010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.543"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>567
                     /gene="ospC1"
                     /locus_tag="ACHU2L_004603"
     CDS             <1..>567
                     /gene="ospC1"
                     /locus_tag="ACHU2L_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR1359108.1"
                     /translation="DKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIR
                     KSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAV
                     TASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQDVISIKHELYDI
                     EYLLSAHNSSCKVLEYFINKGLVDVNTKF"
BASE COUNT          208 a           79 c          106 g          174 t
ORIGIN      
        1 gataaatttt cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt
       61 atagatgtac caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt
      121 gactttatta gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt
      181 gcccctgtgg atctggatag aatcataaac tttgtttttc agccagagta ccatatacct
      241 aggatggtaa gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag
      301 gctgttacag catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct
      361 ctccaggctc tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta
      421 tctaattttg agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt
      481 gagtatctac ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag
      541 ggattggttg atgtaaacac aaagttc
//
LOCUS       DAZPEU010000534          566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZPEU010000534 DAZPEU010000000
VERSION     DAZPEU010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.544"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACHU2L_004604"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACHU2L_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1359109.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="ACHU2L_004605"
     CDS             complement(405..>566)
                     /locus_tag="ACHU2L_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1359110.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAZPEU010000535          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZPEU010000535 DAZPEU010000000
VERSION     DAZPEU010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.545"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHU2L_004606"
     CDS             <1..>557
                     /locus_tag="ACHU2L_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR1359111.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVS"
BASE COUNT          164 a          137 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcagcc
//
LOCUS       DAZPEU010000536          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZPEU010000536 DAZPEU010000000
VERSION     DAZPEU010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.546"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(29..553)
                     /locus_tag="ACHU2L_004607"
                     /pseudo
     CDS             complement(29..553)
                     /locus_tag="ACHU2L_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          155 a          130 c          114 g          154 t
ORIGIN      
        1 aggtgtctgt taaacccgtg gcgattcagg tctgcatagc attatcaaca ttcggatgtg
       61 caactacagc taatgcaaca tcatcccagc ttgcaatagt gctatcagca acaccatctc
      121 cgtcagtatc tacggtgaca aagttagatt taccactata atcgctggca ttacctgtat
      181 taccgaacca accagtagta ccttcatcag tccatgaacc agaggttacg gtagaatctt
      241 taacagtgat ggtgttatta aacacttcgc tgctgtttac gccagtgctg acagtgttgt
      301 cgtcaatatc ttcccactca taaccctgag tcaacgtaat acccgcaacg tgagagttat
      361 tagtgatgga cagatcaact tcctgatcca gagtaatagc cgtaccaatg ttgtaaacgt
      421 ctacaacatg agtatcagcg gcattattgt aagtgccgtt atatgtgtag tgttcgtagt
      481 tgtcgtcgat cgttgaatta tcaacagtca gcgccagacg gtcatggtag taatcatccg
      541 cacgatcagc aca
//
LOCUS       DAZPEU010000537          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZPEU010000537 DAZPEU010000000
VERSION     DAZPEU010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.547"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>553)
                     /locus_tag="ACHU2L_004608"
     CDS             complement(<1..>553)
                     /locus_tag="ACHU2L_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR1359112.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          134 a          119 c          135 g          165 t
ORIGIN      
        1 ccatttaata tcaatctgtt gaaggtgtat tattacatca gtagcatatt ccagactgcc
       61 ttcctggacg atctgatcca taacatcctc acgcacttta tatcttgtgc atttcatttc
      121 aagtgccgtg agtacagcca ttactggtga tggtagctgt gttgttttca ggccgtcgat
      181 ctgactgatc gcttgggcga cggtgttttg ccattctaaa gaacaggtgc tgaagtcaaa
      241 tgtggctgtt ttattatccc gttgcgacac gctacaggct aagcgacgta attgggtcaa
      301 tttgtctgct tgctgaacag gaactccggg aaagcggcga tgacttaacg tggtgtgtac
      361 aacttcgggc ggaaaaacga cagcaatatc tttcgccagc caaggtaaaa cagcatctgt
      421 atttttgtca gccgcacggg tttctgctac gaaagagggt tgttcctgaa taatggtgga
      481 taagtccgct accttgagag agtccgttac cgtttgggaa ggttgcaaag aattatcttt
      541 cgcttgctcc aca
//
LOCUS       DAZPEU010000538          552 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZPEU010000538 DAZPEU010000000
VERSION     DAZPEU010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.548"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..256
                     /locus_tag="ACHU2L_004609"
     CDS             <1..256
                     /locus_tag="ACHU2L_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1359113.1"
                     /translation="KRAGISNFRFHDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQ
                     RYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
BASE COUNT          165 a          122 c          121 g          144 t
ORIGIN      
        1 aaagcgggca ggcattagca acttccgctt ccatgacctg aggcatacct gggcaagctg
       61 gctggttcag tccggtgtct ctcttcttgc gcttaaagag atgggaggat gggaaactct
      121 cgaaatggtt caaagatacg cccacctttc agccgggcat cttaccgagc acgcgagcaa
      181 aatcgatgcg attataagtc gcaatggcac aaatacggca caagaggaga acgtagttta
      241 cttaaatgcg aggtaactta ttgatttaaa tggtgccgat aataggagtc gaacctacga
      301 ccttcgcatt acgaattata agaatccgct tgtaattcaa aggattatcc catcaacact
      361 gcgctcacac gtcccaccac atcaaaacat gtaaagcctt gcaagccatt gtgaggcctt
      421 atgtgtctca gttttgtctc atcagacata gcaagtatcg atcaattgag acttggatga
      481 tagacttcat gcctttcaga gctcattgat taaataaatg ttaaagttat ttgtaaagta
      541 cacatctata gg
//
LOCUS       DAZPEU010000539          551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZPEU010000539 DAZPEU010000000
VERSION     DAZPEU010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.549"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..385
                     /locus_tag="ACHU2L_004610"
     CDS             <1..385
                     /locus_tag="ACHU2L_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFR1359114.1"
                     /translation="NKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHS
                     EMVISYYGGKISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVE
                     RNLSRLNEGMQYAKRHFTGIQTSCL"
     gene            327..>551
                     /gene="mxiN"
                     /locus_tag="ACHU2L_004611"
     CDS             327..>551
                     /gene="mxiN"
                     /locus_tag="ACHU2L_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR1359115.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQT"
BASE COUNT          187 a           69 c          126 g          169 t
ORIGIN      
        1 taataaatat gatctaaatt gcgatattga acctttaagc ggggtaaccg ctatgtttgt
       61 tgccaactgg aatttacttc cagctgttgc ttattttatc ggtagtcaag agtctcggtt
      121 aattaatcat agtgagatgg ttattagtta ttatgggggc aaaatcagta agcaagggga
      181 ggccgctatt cgaagtggtt tttggcatct tattgcgtgg aaggaaaata tatctgttgg
      241 tatatatgaa agaataaact tattatttaa tccaattgct ttggagggaa attatacgcc
      301 tgtagaaaga aatttgtccc gattaaatga aggtatgcaa tatgcaaaaa ggcactttac
      361 cggtatccag acatcatgcc tatgatggtg ttgttataaa gagaattgaa aaggaattat
      421 gtaaaaccat aaaagataga gacactgaga gtaagaagaa agcaatttgt gttataaagg
      481 aggctacgaa aaaagctgaa tcactgcgta tagatgctgt atgtgatggt tatcaaattg
      541 gtatacagac t
//
LOCUS       DAZPEU010000540          550 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZPEU010000540 DAZPEU010000000
VERSION     DAZPEU010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.550"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>550)
                     /gene="intI1"
                     /locus_tag="ACHU2L_004612"
     CDS             complement(<1..>550)
                     /gene="intI1"
                     /locus_tag="ACHU2L_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1359116.1"
                     /translation="VHWVRAFIRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQ
                     ALAALLFFYGKVLCTDLPWLQEIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQL
                     LYGTGMRISEGLQLRVKDLDFDHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARA
                     WWLKDQAEGRSGVALPDALERKY"
BASE COUNT          120 a          188 c          155 g           87 t
ORIGIN      
        1 gatacttccg ctcaagggcg tcgggaagcg caacgccgct gcggccctcg gcctggtcct
       61 tcagccacca tgcccgtgca cgcgacagct gctcgcgcag gctgggtgcc aagctctcgg
      121 gtaacatcaa ggcccgatcc ttggagccct tgccctcccg cacgatgatc gtgccgtgat
      181 cgaaatccag atccttgacc cgcagttgca aaccctcact gatccgcatg cccgttccat
      241 acagaagctg ggcgaacaaa cgatgctcgc cttccagaaa accgaggatg cgaaccactt
      301 catccggggt cagcaccacc ggcaagcgcc gcgacggccg aggtcttccg atctcctgaa
      361 gccagggcag atccgtgcac agcaccttgc cgtagaagaa cagcaaggcc gccaatgcct
      421 gacgatgcgt ggagaccgaa accttgcgct cgttcgccag ccaggacaga aatgcctcga
      481 cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
      541 cccagtggac
//
LOCUS       DAZPEU010000541          544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZPEU010000541 DAZPEU010000000
VERSION     DAZPEU010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.551"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>544
                     /gene="rfaY"
                     /locus_tag="ACHU2L_004613"
     CDS             <1..>544
                     /gene="rfaY"
                     /locus_tag="ACHU2L_004613"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR1359117.1"
                     /translation="EQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVLKVYAPK
                     HKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIE
                     YIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGK
                     KTTAVLKAKDRIDLERHYNIK"
BASE COUNT          193 a           79 c           97 g          175 t
ORIGIN      
        1 gagcaagttc taaatgactt tttaacttgc agggttaaaa cattaaaagt ttttcgttct
       61 attgatgata ctaaagtcat tcttattgac actgctcgtg gacctcttgt acttaaagtt
      121 tatgcgccaa agcataaaat gaccgaacgt ttcttaaaat cttgcataaa aaaagattat
      181 tatgaaaatt taatttatca aaccgatcgg gtacgtggtg aaggcatcca atcaattaat
      241 gactattttt tgctagctga acgtaaaaca ttaaatttcg ctcattatta tattatgctc
      301 attgagtata ttgaaggtgt cgggttaaat gagtatttgg aaatttctga agatctgaag
      361 gatcaattat cagaatcgat aaaagagttg catcagcatg gaatggtgtc tggcgatcct
      421 cataaaggaa attttatagt atccgaaaaa ggtttaagac taattgacct ttcaggtaaa
      481 aaaacaaccg ctgttttaaa agcaaaagat cgcattgatc ttgaacggca ctataatata
      541 aaaa
//
LOCUS       DAZPEU010000542          542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZPEU010000542 DAZPEU010000000
VERSION     DAZPEU010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.552"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>542
                     /gene="ospB"
                     /locus_tag="ACHU2L_004614"
     CDS             <1..>542
                     /gene="ospB"
                     /locus_tag="ACHU2L_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR1359118.1"
                     /translation="NDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSH
                     QLGLGSELIDVQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILN
                     SLPFFKEKESLLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPA
                     DPEHTVKRSSQKKTFIINKE"
BASE COUNT          180 a           94 c           97 g          171 t
ORIGIN      
        1 taatgatgat gacgcattga atgctgctgc ttatttttct gtaaaaaaag cgaaaataaa
       61 aacagtaaac gatactgatt ttaaagagta taataaggtt tatattcttg ggcacggtag
      121 tcctggttct catcaattag gccttggttc ggaacttatt gatgtacaaa caatcatttc
      181 aagaatgaaa gactgtggta ttctaaatgt gaaagatatc cgttttactt catgcggctc
      241 cgctgataaa gtggctccta aaaattttaa caatgcccct gctgaaagtc tttcttgtat
      301 ccttaactct ctgccttttt ttaaggaaaa agaatctttg ctagagcaga taaaaaaaca
      361 ccttgaaaac gatgagtcat tgagtgatgg tctaaaaata tccggctatc atggatatgg
      421 agttcactat ggtcaagagc tttttcccta ctcacattat cgttcaactt caattcctgc
      481 tgatccggag catacagtaa aaagaagctc tcagaaaaag acttttatta ttaataaaga
      541 ac
//
LOCUS       DAZPEU010000543          537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZPEU010000543 DAZPEU010000000
VERSION     DAZPEU010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.553"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..487
                     /locus_tag="ACHU2L_004615"
                     /pseudo
     CDS             1..487
                     /locus_tag="ACHU2L_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          124 a          118 c          136 g          159 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgtga ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatc
//
LOCUS       DAZPEU010000544          536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZPEU010000544 DAZPEU010000000
VERSION     DAZPEU010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.554"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          166 a          105 c           98 g          167 t
ORIGIN      
        1 cttctgggga acgctgaaaa atgaaagcct gagccactat cgttttaaga gccgggatat
       61 ttcgtcagca tacgggaaaa ctgattgagg tcggcctcag ttttgaggcc tttagccagt
      121 tcagccgcaa gggctttaag cttcttttcg tccataattt acctgtctcc gttgttggag
      181 taaacatatc aaaaacaggc aattacacaa tttaaattac agtctcaaaa caaacccaac
      241 ggtcaatata gtttaccagc aagcttacta gcaaagtgct aatcatttgg tatctttcgt
      301 gctattctcc ttttacgtaa gtacttaagg ggaacgtatt tactactttt aaagaaagct
      361 ccgttactat ctgaaatgca atgagataat aaacgattac tctcggttag tatctcatga
      421 tcatttacag actttgcata atcgcgaata ctatataaca gagttttaac attacaggat
      481 gaatagtctc tcttatcaac tcctaataaa agaaggatta tggtcagtcg tttttg
//
LOCUS       DAZPEU010000545          532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZPEU010000545 DAZPEU010000000
VERSION     DAZPEU010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.555"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            381..>532
                     /locus_tag="ACHU2L_004616"
     CDS             381..>532
                     /locus_tag="ACHU2L_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1359119.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          131 a          140 c          115 g          146 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatagg tcttctctat ggtctctcta taggtctctc
       61 tagggtctcc tctaggcatt acccccagaa gctaactcta tggccctcta tggtcctcta
      121 tggccctgta cgtcattctt taggatgccc tgtagtttcg tatagcctga tgcctagaga
      181 gaccctagag agaccctaga gtggctccta gagagaccct cccagtagct ccgcacagct
      241 acatcgtcat catcatagta ttcaatctca aagtcattct gtttgcatcc tgttgtcctg
      301 ctggtctccc ttcgcttggg gactttgagt aggtcagtgg gggtgcgact tcagggtgca
      361 aacaaaaagg aaatcaagcg atgtctcaac aaccacaaca accacaacaa gaaatcagca
      421 tcattaacct cgaccagctt gtgtccatga cctcagttga aattgctgag ctgacaggca
      481 aggagcaccg aaatgtcctt cgtgatattc gtaagatgct cgaagaactt aa
//
LOCUS       DAZPEU010000546          523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZPEU010000546 DAZPEU010000000
VERSION     DAZPEU010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.556"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          169 a           78 c          106 g          170 t
ORIGIN      
        1 atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa atagccctgc
       61 tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg tatctgtcag
      121 cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag caatgtatct
      181 tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc ttacatcacg
      241 actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc acaccgtaat
      301 gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc tcttattttc
      361 cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga tctcatttcg
      421 agtattgaga aatttgtgat ttttaaattt ttcaataagc cctgtaaggg attggggttt
      481 tatgagaaat aaagagacgt attgtctttg tttaaacggt acg
//
LOCUS       DAZPEU010000547          521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZPEU010000547 DAZPEU010000000
VERSION     DAZPEU010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.557"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..95)
                     /locus_tag="ACHU2L_004617"
                     /pseudo
     CDS             complement(1..95)
                     /locus_tag="ACHU2L_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            93..176
                     /gene="tnpB"
                     /locus_tag="ACHU2L_004618"
                     /pseudo
     CDS             93..176
                     /gene="tnpB"
                     /locus_tag="ACHU2L_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          147 a          105 c          134 g          135 t
ORIGIN      
        1 gacttttcgt ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg
       61 atgcgggtgc ggagggtcag gttgttacgt tcaagatata gcatgtgctg gatgaagatc
      121 ccttttctgg tcacctgttt atctttcgtg gccgacgggg tgacactgtt aaaatgtaat
      181 gaacgcatcc cacacaatta aacacggagt agtatgatgg ttaaaagggt gaaactggca
      241 ggtgtggagc ctcgcagctt tccggcatca tactgcccaa tacgggatgc ataaagcgtc
      301 ctcagactgg agactccaca aatgaaatat acaccggttg gcgttgatat ggcaaaacat
      361 gtcattcaga ttcacttcat caatgagcac atgtcagcgc cagtgatata agacggtaat
      421 ggggatgcgg acgatttcct ggactctcag aagatgaaac atgtttactg aagaacaaaa
      481 aattcgcgct attgagcttt atttcaaata tggtaagaag c
//
LOCUS       DAZPEU010000548          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZPEU010000548 DAZPEU010000000
VERSION     DAZPEU010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.558"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..511
                     /locus_tag="ACHU2L_004619"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACHU2L_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZPEU010000549          505 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZPEU010000549 DAZPEU010000000
VERSION     DAZPEU010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.559"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..89)
                     /locus_tag="ACHU2L_004620"
     CDS             complement(<1..89)
                     /locus_tag="ACHU2L_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1359120.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEG"
     gene            complement(427..>505)
                     /locus_tag="ACHU2L_004621"
     CDS             complement(427..>505)
                     /locus_tag="ACHU2L_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFR1359121.1"
                     /translation="MKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          131 a          116 c          105 g          153 t
ORIGIN      
        1 ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc
       61 gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca
      121 ggaaggagtg tccgttaaac tcaggctacc tcactcccgt aaagacagga gtcaagttcc
      181 cgctccgaca taactgcgtg caatggattt tgaaggaacc gtcagcccat tccccggagg
      241 caaacatggg ctggcaaaag tgtagcggta ttaggcacag ctatttagga aagagggacc
      301 atctccatct cattaaatgg accacgccag acaattcact gtcatgctga tgctgcactg
      361 tggggacaga atcactcatg atgacttcga taatcgacga cattattatt tggccgagct
      421 tttaggtcat atctcaaatc gtttgcctaa tattgctcca ttttttaata aaaaatcaat
      481 catttttgaa tctttgcttt tcatt
//
LOCUS       DAZPEU010000550          504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZPEU010000550 DAZPEU010000000
VERSION     DAZPEU010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.560"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..228
                     /locus_tag="ACHU2L_004622"
     CDS             <1..228
                     /locus_tag="ACHU2L_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR1359122.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          170 a          104 c           81 g          149 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaa
//
LOCUS       DAZPEU010000551          503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZPEU010000551 DAZPEU010000000
VERSION     DAZPEU010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.561"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            18..161
                     /locus_tag="ACHU2L_004623"
                     /pseudo
     CDS             18..161
                     /locus_tag="ACHU2L_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          157 a          111 c           99 g          136 t
ORIGIN      
        1 tctagctcag aagagttaat accctcgaga aagacgaaaa ttctccggac aatggctttg
       61 aggtcgggga atataacaca tatgaagcat ataactcaga gaatcaatat tttaccagag
      121 aggactatac gtataattac gaccttttaa atgcaatata gacttaataa cttcttacaa
      181 agtctattat taataccgta gatattcatc ttaccctgtg caccaaaagc aaaaacttat
      241 cgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct gatgcgctac gcttatcagg
      301 cctacgccat ctctgcaata tattgaattt gcgtgctttt gtaggcagga taaggcgttc
      361 acgcagcatc cggcatgaat aaagcgcact caacaatctg aaacccgccg gaacggttta
      421 ttacgtacat caggtgaaac tgaccgataa gccgctttct tttgggtata gtgtcgtgga
      481 cagtcattca tctttctgcc cct
//
LOCUS       DAZPEU010000552          498 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZPEU010000552 DAZPEU010000000
VERSION     DAZPEU010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.562"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(37..432)
                     /locus_tag="ACHU2L_004624"
     CDS             complement(37..432)
                     /locus_tag="ACHU2L_004624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359123.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLLNEVA"
BASE COUNT          148 a          118 c          106 g          126 t
ORIGIN      
        1 atgccattct atttcaggaa ggagtgtccg ttaaactcag gctacctcat ttaacagact
       61 tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat tgaggttcca
      121 gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat taaaacgcag
      181 ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc gaccgatgac
      241 gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac gcaccatccc
      301 gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc tggctttctt
      361 atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt gggtgtttga
      421 gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa ctatccgtca
      481 tgcctgagat tgcgtcgg
//
LOCUS       DAZPEU010000553          493 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZPEU010000553 DAZPEU010000000
VERSION     DAZPEU010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.563"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>493
                     /gene="mxiD"
                     /locus_tag="ACHU2L_004625"
     CDS             <1..>493
                     /gene="mxiD"
                     /locus_tag="ACHU2L_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR1359124.1"
                     /translation="NLYDHRYPIRGNISDKTFYISGPPALVELVANTATLLDKQVSSI
                     GTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLLNNGKALSNRQAQNDPM
                     PPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSILVKGNDQQIQIIRDIIT
                     QLDI"
BASE COUNT          169 a           75 c           93 g          156 t
ORIGIN      
        1 aacctttatg atcacagata tcctataaga ggaaacatta gtgataaaac attctatata
       61 tctggtcctc cggcattggt tgaactggta gctaatacag caactttgct tgataagcag
      121 gtttcttcta ttgggacaga taaagttaat tttggggtta taaaattaaa aaatacattt
      181 gttagtgaca gaacttataa tatgagaggg gaggatattg tcatcccagg ggtggctact
      241 gttgtagaac gtttattaaa taatggtaag gctctgagta acagacaagc ccaaaatgat
      301 ccgatgccac cctttaatat tacacagaaa gtatctgagg atagtaatga cttttcattt
      361 agttcagtta ccaatagttc tatattagag gatgtatcac ttattgcata tccagaaacc
      421 aattctatat tagtcaaagg taatgatcag caaatacaga ttattcgcga tattatcact
      481 cagttagata tag
//
LOCUS       DAZPEU010000554          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZPEU010000554 DAZPEU010000000
VERSION     DAZPEU010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.564"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            2..>489
                     /gene="mxiJ"
                     /locus_tag="ACHU2L_004626"
     CDS             2..>489
                     /gene="mxiJ"
                     /locus_tag="ACHU2L_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR1359125.1"
                     /translation="MLIGCEQREELISNLSQRQANEIISVLERHNITARKVDGGKQGI
                     SVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAEKARLYSAIEQRLE
                     QSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESELLVSNIKRFLKNT
                     FS"
BASE COUNT          160 a           76 c          104 g          149 t
ORIGIN      
        1 gatgttgatt ggatgtgagc aacgtgaaga gttaatttct aatttatctc aaagacaggc
       61 aaatgaaata atatctgtgc tagaacgcca taatattact gctagaaaag ttgatggagg
      121 taaacagggg atctcggtac aagtcgaaaa ggggacattt gcatcggcag ttgatttgat
      181 gcgcatgtac gatttgccaa atccggagag agttgatatc tcacaaatgt ttcctacaga
      241 ttcattagtg tcttctccaa gagctgaaaa ggcccgttta tatagtgcta ttgagcaacg
      301 gctggaacag tctttagttt ctattggtgg tgttatttcg gcaaaaatac atgttagcta
      361 tgatcttgaa gaaaaaaata tatcttcaaa accgatgcat atatcagtaa tcgctatata
      421 tgactcaccg aaagagtctg aactattagt tagtaatatt aagcgatttt tgaaaaacac
      481 cttttctga
//
LOCUS       DAZPEU010000555          484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZPEU010000555 DAZPEU010000000
VERSION     DAZPEU010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.565"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          141 a           84 c          114 g          145 t
ORIGIN      
        1 tcagtgagag tggtgaaaat aagggtttac tcgccatcag gctgagcata attctcatca
       61 tgaaatatgt ttcttggttt gtggcttgta actggtcact cctgatgtcg atctggagag
      121 gcttgttgat gttggtgttt tcaggatgat gtttcactta gtttgtttgc cgtatcgccc
      181 ggcgaatagc tgtgattgag gaaggcttaa gtcgtagtga ccaaagctat atttaccaac
      241 ggatgtagat gaaaaagcgt cgcctgcgtt cccccatatc tccaggataa aaaggaatgt
      301 aacaatctca tggcctaagc tgacgaacca ggcaggaata atcgctaggg atctaagaat
      361 tagcatgata atagccacta agaaattact gcgttccatg aaatagccat tttgtggcaa
      421 atgcagttaa ctaataatgt catatttgag acggctagtt taacaaatat taaattttgc
      481 tgaa
//
LOCUS       DAZPEU010000556          484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZPEU010000556 DAZPEU010000000
VERSION     DAZPEU010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.566"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..484
                     /locus_tag="ACHU2L_004627"
                     /pseudo
     CDS             1..484
                     /locus_tag="ACHU2L_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          178 a           81 c           95 g          130 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaa
//
LOCUS       DAZPEU010000557          482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZPEU010000557 DAZPEU010000000
VERSION     DAZPEU010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.567"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(199..>482)
                     /locus_tag="ACHU2L_004628"
     CDS             complement(199..>482)
                     /locus_tag="ACHU2L_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359126.1"
                     /translation="GVVNISSDNLQNILSKPREHTLNCLGWRFFVQRSVCVSWKSDFA
                     WTGIMLLFRGEELAFSSAFSPVTIRKIMKCPPPGRRTVEKRTRQQKHWG"
BASE COUNT          144 a          115 c           95 g          128 t
ORIGIN      
        1 ccccgacaga cctgtgctat cggaaagaaa aaaggccatc agcaaaactc tgatgacctg
       61 aatcaccttt accaatattg tatgaaaaaa cacgcacgac taattgacaa taattttcat
      121 ttccattaaa aaagatcgcg tagcattctt aattcatgag gaactttacc cgccagcaat
      181 ctgagtagcg aaagctgttc atccccaatg tttttgctgg cgggtccttt tttctacggt
      241 cctcctcccc ggaggggggc atttcattat ttttctaatc gttactggtg agaaggcgga
      301 agaaaaagcc agttcttcgc ccctaaataa cagcatgatt ccagtccatg caaaatctga
      361 tttccagctc acacataccg agcgctgtac aaaaaaccgc cagccaaggc agttaagagt
      421 gtgttcccgg ggtttgctta gaatattttg taagttgtcc gaagagatat ttacaacacc
      481 ag
//
LOCUS       DAZPEU010000558          479 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZPEU010000558 DAZPEU010000000
VERSION     DAZPEU010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.568"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..139
                     /locus_tag="ACHU2L_004629"
     CDS             <1..139
                     /locus_tag="ACHU2L_004629"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1359127.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
     gene            172..479
                     /locus_tag="ACHU2L_004630"
                     /pseudo
     CDS             172..479
                     /locus_tag="ACHU2L_004630"
                     /inference="COORDINATES: protein motif:HMM:NF014380.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          139 a          107 c          118 g          115 t
ORIGIN      
        1 ggcaggctta aagcctgaca ctatgcgtaa cgtatttcgc tgccatatac cacgatacga
       61 agcaatcatt gctagatatt tggatatgga accagcccag atttggcctt ctcggtatat
      121 ggagaaaaca tcatgttaac ccattgggta acagcgcaag aatgcgtagg aatgtcatgc
      181 tttcctaaac atgaggtgag tattcgccgc aatcttgaaa agcttgcagc caatcatcca
      241 gaactacgtc gcaagcgtac gggttcaaag gctttggaat ttcatatatc agttctacca
      301 gcggcagcac gtgctgagtt gttattaaaa tctgagcaaa tagaaacaag ctaggggtat
      361 tttgaaatcg cccgccccac gctggaagcg cacgattacg atcgtgaggc actgtggagc
      421 aaatgggata aagccagcga ttcccagcgc agacttgctg aaaaatggtt gcctgcggt
//
LOCUS       DAZPEU010000559          473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZPEU010000559 DAZPEU010000000
VERSION     DAZPEU010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.569"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..473
                     /locus_tag="ACHU2L_004631"
                     /pseudo
     CDS             1..473
                     /locus_tag="ACHU2L_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAZPEU010000560          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZPEU010000560 DAZPEU010000000
VERSION     DAZPEU010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.570"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            3..>472
                     /locus_tag="ACHU2L_004632"
     CDS             3..>472
                     /locus_tag="ACHU2L_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR1359128.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZPEU010000561          465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZPEU010000561 DAZPEU010000000
VERSION     DAZPEU010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.571"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>465
                     /locus_tag="ACHU2L_004633"
     CDS             <1..>465
                     /locus_tag="ACHU2L_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1359129.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASA"
BASE COUNT          147 a           96 c          102 g          120 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agcta
//
LOCUS       DAZPEU010000562          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZPEU010000562 DAZPEU010000000
VERSION     DAZPEU010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.572"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>459
                     /locus_tag="ACHU2L_004634"
     CDS             <1..>459
                     /locus_tag="ACHU2L_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1359130.1"
                     /translation="SAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWVSE
                     MADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSSL
                     PAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          116 a          109 c          141 g           93 t
ORIGIN      
        1 ccagtgcagg gttacttgca cggatcctgg tcagcaaata tatggaacat atccctttat
       61 atcgccagtc agaaatatac gcgcgacagg gcgtggagct gagccgtaat accatggtgc
      121 gctgggtatc agaaatggca gacaaactcc gtcctctgta tatagcgctg aatgactatg
      181 ttctggaggc aggaaaggtg cacgcagatg acactccggt gaaagtactg gccccgggga
      241 acggaaagac gaaaacgggt cgtctgtggg tatacgtcag ggatgatcgt aatgcgggtt
      301 catccctgcc ggcagccgtc tggttcgcgt attcggcaga tcgcaaagga gaacatccgc
      361 agctccacct ggcaaagtat cagggcgtac tgcaggctga tgcctatgca ggttataacg
      421 tactgtacga aacgggccgg gtgaaggaag ccgggtgcc
//
LOCUS       DAZPEU010000563          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZPEU010000563 DAZPEU010000000
VERSION     DAZPEU010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.573"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>459)
                     /locus_tag="ACHU2L_004635"
     CDS             complement(<1..>459)
                     /locus_tag="ACHU2L_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR1359131.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZPEU010000564          456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZPEU010000564 DAZPEU010000000
VERSION     DAZPEU010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.574"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..456
                     /locus_tag="ACHU2L_004636"
                     /pseudo
     CDS             1..456
                     /locus_tag="ACHU2L_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          160 a           84 c           75 g          137 t
ORIGIN      
        1 atttagtccg tcgaaaaatg aatgaccttc ctgaaagccc cataggaaat aacctcgaag
       61 cactagcacc aggtatagaa aaactaaaac agacctctat tcaaatggtc actttactta
      121 acgcgttaca accaagtgga aaatgcatta tcactggtga ttttcaaaaa gaattagcgt
      181 acttacaaaa tgtaattctt tataatgact cgtctcttcg tctggatttt ttaggttata
      241 acacccaaat tattcaacga tcggacaata cctgcgaact taccattaat gaaccgttaa
      301 aaaactagga aatatctaca ggcaatatca atgttaattg cccattaaaa gatatttaca
      361 atgaaatcag gaggttgaac gtaatcttta gttgtgggac tggagatata gttgatctat
      421 cctctctgga cttacgtaat gtcgatttag attatt
//
LOCUS       DAZPEU010000565          455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZPEU010000565 DAZPEU010000000
VERSION     DAZPEU010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.575"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            37..>455
                     /gene="ospC2"
                     /locus_tag="ACHU2L_004637"
     CDS             37..>455
                     /gene="ospC2"
                     /locus_tag="ACHU2L_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR1359132.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDF"
BASE COUNT          162 a           84 c           73 g          136 t
ORIGIN      
        1 actgcagata aaaacgcaca taatggggga tgttttatga aaatacctga agcagtaaat
       61 catattaatg ttcagaacaa tattgatctt gttgatggaa aaataaatcc gaacaaagat
      121 acaaaagcat tacaaaaaaa catatcatgc gtaacaaact catcctcttc tggcataagt
      181 gaaaaacatt tagatcactg tgctgatact gtgaaaagct ttttaaggaa gtctatagca
      241 gcgcaaagtt atagtaaaat gttctctcaa gggaccagtt tcaaatcatt aaatctttct
      301 attgaagcgc catctggagc acggtcatca tttcggtcac ttgagcacct cgataaggtg
      361 tccagacact atctttctga aataatacaa aaaactcatc ctctttcttc ggatgaacgt
      421 cacttgctct ctattattat taattctgat tttaa
//
LOCUS       DAZPEU010000566          455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZPEU010000566 DAZPEU010000000
VERSION     DAZPEU010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.576"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..228
                     /locus_tag="ACHU2L_004638"
     CDS             <1..228
                     /locus_tag="ACHU2L_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFR1359133.1"
                     /translation="VFIHYTGITKPWHDWANYASADYFRNIYNISPWRNIPYKKAVKK
                     HEHKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
     gene            237..>455
                     /locus_tag="ACHU2L_004639"
     CDS             237..>455
                     /locus_tag="ACHU2L_004639"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(glucosyl)LPS
                     alpha-1,2-glucosyltransferase"
                     /protein_id="HFR1359134.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLF"
BASE COUNT          152 a           72 c           78 g          153 t
ORIGIN      
        1 gtcttcatac attatactgg tataactaag ccatggcatg attgggcgaa ctacgcctct
       61 gcagattatt ttcgtaatat ttataatata tcaccatgga gaaatatacc ttataaaaaa
      121 gctgttaaaa aacatgagca caaagaaaaa tataaacact tgctttacca gaaaaaattt
      181 ctcgatggtg tttttacagc aattaaatat aatgttatga aaggttaata tacgagatgg
      241 ttgataaaat aatatttacg gttactccta ttttttcaat tcctcctcgc ggagcggctg
      301 ctgttgaaac gtggatgtat caggttgctc agcgtaccaa ttttccaaat cgtattgttt
      361 gtatcaaaaa tccaggatac tcaaattaca cctttgttaa tgataattgt agtattcata
      421 gggttggatt cagtcgcata tataagcggt tattt
//
LOCUS       DAZPEU010000567          448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZPEU010000567 DAZPEU010000000
VERSION     DAZPEU010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.577"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>448)
                     /locus_tag="ACHU2L_004640"
     CDS             complement(<1..>448)
                     /locus_tag="ACHU2L_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359135.1"
                     /translation="KRQQFRWLAINNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDD
                     TMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLLMALGLQLGDVRLAQFLANICFFV
                     IACGLVGCSWEITKTYYPGIIATILLFTINPLKDHLGYAYIDLTLSL"
BASE COUNT          179 a           85 c           74 g          110 t
ORIGIN      
        1 caaagatagc gttaaatcaa tatatgcata accaagatga tctttaagtg gattgatagt
       61 aaatagtagg attgtagcaa taatgcctgg ataataagtt ttcgtaattt cccatgaaca
      121 gccaacaagt ccacaggcta ttacaaaaaa acagatattt gctaaaaact gagctaacct
      181 tacatctcct aactgtaaac ctaaagccat taaaagatct gcattttgcg ggaacagagg
      241 aaaacgcaaa tattgttcaa caacaattgc atggtgctca actaatgaac gagccaaagg
      301 taagtgatac atagtatcat cccaatcacc gggagctcgc agggacgata acaatgtaac
      361 cacaaaaatc aaaacaagaa ataaaataac aaacagcgta ctgaactcaa tattatttat
      421 tgccagccat ctaaattgct gacgttta
//
LOCUS       DAZPEU010000568          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZPEU010000568 DAZPEU010000000
VERSION     DAZPEU010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.578"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            213..>443
                     /locus_tag="ACHU2L_004641"
     CDS             213..>443
                     /locus_tag="ACHU2L_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR1359136.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZPEU010000569          441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZPEU010000569 DAZPEU010000000
VERSION     DAZPEU010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.579"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..291)
                     /locus_tag="ACHU2L_004642"
     CDS             complement(<1..291)
                     /locus_tag="ACHU2L_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359137.1"
                     /translation="MNAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNS"
BASE COUNT          142 a           73 c           65 g          161 t
ORIGIN      
        1 actattatat tcttgaatat ctatagatgg ctcactattg taaaaccata ttttctccat
       61 ctgaagacta tgttcattta ccctccagtg accatgttgc aggaaataga tgatatgcat
      121 gccaaaatcg ttatctattt gcgaatcatt atccagctta tcaatttcaa ctatctttga
      181 acgatgtgtt ttatcaacct tgctatggtg ttgatgatta ttaactttat ctgaatgctt
      241 atctttatgg tttgaaggga tgtacttaaa tatattgtca attgcgttca taaataaccc
      301 gcgatatttt tgacctgtta gtaaaaaata aatgatacat gtctgaaaga catatttacg
      361 acgatataaa acaataatac ttatatgttc gctgatttta agcaccgaga ctatccggtc
      421 ttttactaaa taatggaaaa c
//
LOCUS       DAZPEU010000570          439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZPEU010000570 DAZPEU010000000
VERSION     DAZPEU010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.580"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          151 a           59 c           77 g          152 t
ORIGIN      
        1 tttatatttt tcactagaac aatgagtaga tcaaaattcc caaaactccc aaaaacataa
       61 gattattcgc aaagctgatt ttgcagaata atgagaactt ttaagatttg tcgaggatca
      121 attttaagca atttatctcc acttgttaca taaaagatgc cgttaggtta ataagttaat
      181 cttgctataa cagtgttcgt tactgcctat taataaagat atttgggata gggtaacgtg
      241 gatgaggatt cgaaatcatc cgtcttgcgg aaagtgaaat gttggcagat gttaatatta
      301 tgtatgtatt ttaactccaa caacacacca atcattttgt tagaaataag aaaatttatg
      361 gttaaataat ttggtgatta tgaagcatta aatcgtaatg ccaaatattg ttcgctttgg
      421 agagtattat tgcctgttt
//
LOCUS       DAZPEU010000571          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZPEU010000571 DAZPEU010000000
VERSION     DAZPEU010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.581"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..269
                     /locus_tag="ACHU2L_004643"
     CDS             <1..269
                     /locus_tag="ACHU2L_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1359138.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>434
                     /gene="tnpB"
                     /locus_tag="ACHU2L_004644"
     CDS             266..>434
                     /gene="tnpB"
                     /locus_tag="ACHU2L_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1359139.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKI"
BASE COUNT          109 a          114 c          122 g           89 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atac
//
LOCUS       DAZPEU010000572          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZPEU010000572 DAZPEU010000000
VERSION     DAZPEU010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.582"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            35..434
                     /locus_tag="ACHU2L_004645"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHU2L_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZPEU010000573          431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZPEU010000573 DAZPEU010000000
VERSION     DAZPEU010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.583"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..149
                     /locus_tag="ACHU2L_004646"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACHU2L_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(169..>431)
                     /locus_tag="ACHU2L_004647"
     CDS             complement(169..>431)
                     /locus_tag="ACHU2L_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HFR1359140.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          110 a          104 c          101 g          116 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc aggctgaggg atccccacaa aattactgct aataaaccag
      181 caccatattt cggtaggttc tggacagatg gtttagaact gtgctcagta ctgttcgtct
      241 taaaataagt ggcgagtgtc gaagaacatt cttcgctaac gtcagatatg agataacccg
      301 ccgggattta atgctgttag cctgatattt caggtgtaac cctttatttt cagcgtgata
      361 accgagtaac cacattacga tagtgctcaa cgttgccagc agactcagaa ccagaattct
      421 tcctgttgaa c
//
LOCUS       DAZPEU010000574          429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZPEU010000574 DAZPEU010000000
VERSION     DAZPEU010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.584"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            12..398
                     /locus_tag="ACHU2L_004648"
                     /pseudo
     CDS             12..398
                     /locus_tag="ACHU2L_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          139 a           66 c           87 g          137 t
ORIGIN      
        1 taggttattt tatgtatatt gaacagcatt ctcgctatca aaataaagct aataacatcc
       61 aattaagata tgatgataag cagtttcata caacggttat caaagatgtt ctattatgga
      121 ttgaacataa tttagatcag tctttactgc ttgatgatgt ggcgaataaa gcgggttata
      181 ccaagtggta ttttcagcgg ctgttcaaaa aagtaacagg ggtcacactg gctagctata
      241 ttcgtgctcg tcgtttgacg aaagcggctg ttgagttgag gttgacgaaa aaaactatcc
      301 ttgagatcgc attaaaatat caatttgatt cccaacaatc ttttacacgt cgatttaagt
      361 acatttttaa ggttacacca agttattatc ggcgtaataa attatgggaa ttggaggcaa
      421 tgcactgag
//
LOCUS       DAZPEU010000575          427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZPEU010000575 DAZPEU010000000
VERSION     DAZPEU010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.585"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..427)
                     /locus_tag="ACHU2L_004649"
                     /pseudo
     CDS             complement(1..427)
                     /locus_tag="ACHU2L_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          128 a           59 c           82 g          158 t
ORIGIN      
        1 aggtcatgtt tttaactttt atattcctct ggtttacaac ttcctctgtc ttgtaagtgt
       61 gtatattata tggggtatat gcttttttaa tagtatagtg aaacaattcg ttatccgcat
      121 ctattccgtc agagtgaact tcaaatgcac caggcgaaag aattgccagg ccagtattaa
      181 gcttaagtgc tggggaaaac aaattaaata acatgagctg catagtaggt caattgttct
      241 tcccagaaac atttaaaaaa tttaagtttt tgctctgttt tattatgggg aataaagttc
      301 caatgtcgga tgtattatta gtatcaatgt gggttatttt attattgtcc agtcttaaac
      361 ttttgataga tggaagataa gtcaggaaat caagatttat atatttcaac tggttgttag
      421 cagcgtt
//
LOCUS       DAZPEU010000576          425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZPEU010000576 DAZPEU010000000
VERSION     DAZPEU010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.586"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          132 a           74 c           81 g          138 t
ORIGIN      
        1 tggtggattt tttgtttatc accatgtaaa aaaaattgag tagtaaaagc gttgactgct
       61 aatcctcgct gtttttattg aaacgattaa aaatacgctg aatattttcg gccagtcact
      121 catattcaca taaaattttg accatcatgc cgcatccctt agttgcacaa ttcattttca
      181 atgatatgaa tattactaat ttcaccagat aattttctcg tggtcagcag ggtatgagtt
      241 ttcaccgtaa ttttaaggaa acacagtgcg tggggattga ataacagata gccgacggaa
      301 ttaccccacc ggctgaaagt caagttcaac gaagattttt attatctgaa ttgggcagtt
      361 tacatcagtg ttcgatgttt atgaagacga tagatggcga gaaattatat cccgaatagc
      421 ttcat
//
LOCUS       DAZPEU010000577          412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZPEU010000577 DAZPEU010000000
VERSION     DAZPEU010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.587"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..412
                     /locus_tag="ACHU2L_004650"
                     /pseudo
     CDS             1..412
                     /locus_tag="ACHU2L_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          100 a          114 c          121 g           77 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tc
//
LOCUS       DAZPEU010000578          410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZPEU010000578 DAZPEU010000000
VERSION     DAZPEU010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.588"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>410
                     /locus_tag="ACHU2L_004651"
     CDS             <1..>410
                     /locus_tag="ACHU2L_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1359141.1"
                     /translation="KLYHIDSEPLKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLD
                     ADIVCKGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMYINL
                     REWLKQRLTEKFFDLLSDESIIKKLKYPDQDILN"
BASE COUNT          132 a           51 c           80 g          147 t
ORIGIN      
        1 ttaaattata tcatattgat tctgagccat tgaaggcgct acctcaatca aatatctggc
       61 cagtatctat ttattatcgt ttgctttcat ttgattattt ttctgcgcga ttggattcat
      121 tattatatct tgatgctgat atcgtctgta agggttcatt gaacgagtta atagcattag
      181 agtttaaaga tgaatatggg gcagtggtaa ttgatgtaga tgctatgcaa agtaaaagcg
      241 ctgagcgttt gtgtaatgag gattttaacg gtagctattt taactctggt gtaatgtata
      301 ttaatttacg ggaatggtta aaacaaagac taacggaaaa attctttgat ctattatcag
      361 atgagtcaat tataaaaaaa ttaaagtacc cggatcaaga tattttaaac
//
LOCUS       DAZPEU010000579          409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZPEU010000579 DAZPEU010000000
VERSION     DAZPEU010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.589"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            155..340
                     /locus_tag="ACHU2L_004652"
                     /pseudo
     CDS             155..340
                     /locus_tag="ACHU2L_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          118 a           97 c          108 g           86 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaagg
//
LOCUS       DAZPEU010000580          391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZPEU010000580 DAZPEU010000000
VERSION     DAZPEU010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.590"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT           98 a           85 c           78 g          130 t
ORIGIN      
        1 tttaatatgt tgtatggttt tcactctgaa atcccctgaa tttaatcacg tttcctttac
       61 tgaaggcaat tttaaccgta gtatcaatta ttaacccttg ggcttgatat ccctctcctc
      121 ttctgggcac gggggatgtc agattactta ttacagaaac caaaagaatt gtaagcgtca
      181 tctcagtgtc gtctatttca atgtctgatg attggcgatg atagagtcct cttataacgt
      241 taatggactc tatcaatgtc agattctctt cgcccccgca ggattcgtgc ctcttactct
      301 atgactctat ggagtttaag cttgatctta ttgaaaaatc ttaccagcta ggggcctgcg
      361 tggcccaact ggccagagag tacgggatta a
//
LOCUS       DAZPEU010000581          390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZPEU010000581 DAZPEU010000000
VERSION     DAZPEU010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.591"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(42..>390)
                     /locus_tag="ACHU2L_004653"
     CDS             complement(42..>390)
                     /locus_tag="ACHU2L_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1359142.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          120 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt
//
LOCUS       DAZPEU010000582          385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZPEU010000582 DAZPEU010000000
VERSION     DAZPEU010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.592"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            308..>385
                     /locus_tag="ACHU2L_004654"
     CDS             308..>385
                     /locus_tag="ACHU2L_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1359143.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQR"
BASE COUNT          121 a           70 c           78 g          116 t
ORIGIN      
        1 taatatgttg tacgattttc actctgaaat cccctgagtt taatcacact tcctttactg
       61 aaaggaatta ttgcaacagt attatttgtt tttggatata tatcttgcgc cttgttatgt
      121 agttaatgct cagaatatga tagataaaat agagacgcaa aacagggcat gctcataaca
      181 tgatgataac ataaaaaatt agtaacaata aatgaaagaa tcatgggcgc tcaacaaaaa
      241 gagggtaatg ctgccaactt actgatttag tgtatgatgg tgattttaag gtgcttgcgt
      301 agcttccatt tccatcagat gtccttcctg ctccgctact gaaggcgtgg tgcgtaacgg
      361 caaaagcact gccggacatc agcgc
//
LOCUS       DAZPEU010000583          384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZPEU010000583 DAZPEU010000000
VERSION     DAZPEU010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.593"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..252)
                     /locus_tag="ACHU2L_004655"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="ACHU2L_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           88 a          123 c           86 g           87 t
ORIGIN      
        1 ctcactgtct ccgttatggc gtacaccaca tcttccagta atcgccgctt caccggactc
       61 aggaccccgt atcaccatcc ggaacccctt atccgccaca tgccaggaga acctttccat
      121 aaactcgtcg ccactcatca caaggttttc ttctcgtttt gtccggtggc tgttgtaacg
      181 caggccaatt tcatcctgac cggcataatg ttccaggcgg ctcatctgca ccggctgctt
      241 ctccagccag ttaagccatc ggtttccctg ctgaaaaata tcggcaaaac ggggaagcat
      301 cagaaaggcg gaataacgcc atccggccag tgaaccgtac cgcattcagg gcaatacatg
      361 ccgtcggcgt tgataccggc agga
//
LOCUS       DAZPEU010000584          377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZPEU010000584 DAZPEU010000000
VERSION     DAZPEU010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.594"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>377)
                     /locus_tag="ACHU2L_004656"
     CDS             complement(<1..>377)
                     /locus_tag="ACHU2L_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1359144.1"
                     /translation="SRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDV
                     IRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRA
                     KSLTFYGINGPIYHTRYCYWPIS"
BASE COUNT           86 a          100 c          105 g           86 t
ORIGIN      
        1 ggatatgggc cagtagcagt agcgggtgtg ataaatgggg ccgttaatgc cgtaaaaagt
       61 aagggatttg gctctgtacc gcggttctgt tgtctcaggg cgtgcatcag tccaccggat
      121 gctgagcacc ccttcaaacc gtgtgtcggg tatgatgatg ccgtaggggc cagcaacgga
      181 atattcacct ggcagcgcat tccttaccca ggccaggaaa tcactcttag tgtcaaaacg
      241 gataacatct tcaggcagaa aagcacaccc aaagccgaat gcgccgggta tcgccagacg
      301 gccttttgtc cggtcgtaaa tgtcgctctg tgcttccatc gtggccgcac ttttcagccc
      361 cagattatcc cgggact
//
LOCUS       DAZPEU010000585          375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZPEU010000585 DAZPEU010000000
VERSION     DAZPEU010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.595"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>375
                     /locus_tag="ACHU2L_004657"
     CDS             <1..>375
                     /locus_tag="ACHU2L_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1359145.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZPEU010000586          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZPEU010000586 DAZPEU010000000
VERSION     DAZPEU010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.596"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHU2L_004658"
     CDS             <1..>374
                     /locus_tag="ACHU2L_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1359146.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAZPEU010000587          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZPEU010000587 DAZPEU010000000
VERSION     DAZPEU010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.597"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          141 a           74 c           62 g           95 t
ORIGIN      
        1 aactaactaa cctaaccaga cagatgatca ctgtataaaa acttcagtta atactcattt
       61 gttttgtcga ctcagtatat aatgagtgac cagtaatcag gataatcaca cttacataac
      121 ctgtaaaaaa gaacaagaag aataagccac taacataatg caaaatggca tctgaatcga
      181 tcgcagtttg gcattcgaat cgatcatgat ttggcccaat ccgtgatcga atagagcgga
      241 atacgcaaca taattcacca gacaagagct tacaccgaat tacagattat gtattaatgt
      301 caaaactggc atattttaca gatacaatga acatgcaacc catcaatcta ataggggcgg
      361 tatgtacatc tg
//
LOCUS       DAZPEU010000588          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZPEU010000588 DAZPEU010000000
VERSION     DAZPEU010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.598"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHU2L_004659"
     CDS             complement(<1..311)
                     /locus_tag="ACHU2L_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1359147.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZPEU010000589          367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZPEU010000589 DAZPEU010000000
VERSION     DAZPEU010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.599"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            106..>367
                     /locus_tag="ACHU2L_004660"
     CDS             106..>367
                     /locus_tag="ACHU2L_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1359148.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFA"
BASE COUNT          117 a           55 c           55 g          140 t
ORIGIN      
        1 taataaatta atattgaact cgttgattaa taatcataaa ctttcaggca ttcatatttg
       61 caatgagtgt ttaaaaatca cacaaaactg gagagcctac tttaaatgta taataacaaa
      121 atcgacagca acagaaaaat aagacatgat tgggttgatg ctttaaaatt tctcggtatt
      181 tttgctatat atttaggtca ccttgggttg ggggctggca aattgtatcc ttttgttttt
      241 agttatcacg tcccactatt ctttttcgct gctggttttt ttactatcaa aaaaaatgac
      301 ttatctgttt tcgattatat caagtcaaag ttttacagat taatgattcc atatttcact
      361 tttgcat
//
LOCUS       DAZPEU010000590          363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZPEU010000590 DAZPEU010000000
VERSION     DAZPEU010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.600"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          133 a           55 c           72 g          103 t
ORIGIN      
        1 gtgttggcag ttgaggaaca gaatctgcca gttgaggttt tgccatagaa aatgcgacaa
       61 taagtattgg aagggtggca aacactttca tttgccaatt tcctttaata taaatgaaaa
      121 cacataatgt aattactaaa gttgatagtg tgtacgataa aaggtgattg tcccatccag
      181 cattagcgat attccaaggg acaaatgaaa aaagaaatac attagatgat attatagatg
      241 acataacatc aaaactgaat cctaatggag catgtgatct tgttccatta aatgtaagaa
      301 ttattgatgt tgcctgtatc agcccacaca agatcattgc caaatatggc agtcgagtat
      361 aaa
//
LOCUS       DAZPEU010000591          351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZPEU010000591 DAZPEU010000000
VERSION     DAZPEU010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.601"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>351)
                     /locus_tag="ACHU2L_004661"
     CDS             complement(<1..>351)
                     /locus_tag="ACHU2L_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359149.1"
                     /translation="TTSLECTSTLTRNAKTDHSLYYSANLSVILKKDGSGSFTIVGLT
                     DEDTPRKFSHSYFFTYKIDSNGRISGNAKAKVSGLENQIKDENFRLNFLDASLTGKGN
                     ARLSKFNNVYIFSIP"
BASE COUNT          107 a           66 c           62 g          116 t
ORIGIN      
        1 gggatactaa aaatatagac attattaaac ttgcttagcc tcgcattgcc tttacctgtt
       61 aaagatgcat cgagaaaatt gagtctgaag ttctcatcct ttatctgatt ttccagtccc
      121 gaaactttgg ccttagcatt acctgagatc cgtccgttac tatctatttt gtaggtaaaa
      181 aaatacgaat gggaaaattt cctcggtgta tcttcatcag ttaagccaac aatcgtaaaa
      241 ctaccgctac catctttttt taaaatgact gaaaggttag cagagtagta taaagaatga
      301 tctgttttag catttcttgt taacgtcgac gtacattcaa gagacgttgt g
//
LOCUS       DAZPEU010000592          344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZPEU010000592 DAZPEU010000000
VERSION     DAZPEU010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.602"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>344
                     /locus_tag="ACHU2L_004662"
     CDS             <1..>344
                     /locus_tag="ACHU2L_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1359150.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZPEU010000593          340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZPEU010000593 DAZPEU010000000
VERSION     DAZPEU010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.603"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..171
                     /gene="xisR"
                     /locus_tag="ACHU2L_004663"
     CDS             <1..171
                     /gene="xisR"
                     /locus_tag="ACHU2L_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR1359151.1"
                     /translation="LMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWY
                     NYPKINQFIKDS"
     gene            173..>340
                     /locus_tag="ACHU2L_004664"
     CDS             173..>340
                     /locus_tag="ACHU2L_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR1359152.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           64 c           82 g           86 t
ORIGIN      
        1 ctgatgcttc gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg
       61 gtggaaggct tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg
      121 attatctggt acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa
      181 attaccaaca ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg
      241 aaaacgatgt cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc
      301 cgggaattta agagcgttga tagttcatca aatcagttcc
//
LOCUS       DAZPEU010000594          335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZPEU010000594 DAZPEU010000000
VERSION     DAZPEU010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.604"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>335
                     /locus_tag="ACHU2L_004665"
     CDS             <1..>335
                     /locus_tag="ACHU2L_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1359153.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLA"
BASE COUNT           92 a           79 c           78 g           86 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcgg
//
LOCUS       DAZPEU010000595          334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZPEU010000595 DAZPEU010000000
VERSION     DAZPEU010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.605"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(43..330)
                     /locus_tag="ACHU2L_004666"
     CDS             complement(43..330)
                     /locus_tag="ACHU2L_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359154.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           86 c           67 g           90 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgtc
//
LOCUS       DAZPEU010000596          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZPEU010000596 DAZPEU010000000
VERSION     DAZPEU010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.606"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            51..>332
                     /locus_tag="ACHU2L_004667"
     CDS             51..>332
                     /locus_tag="ACHU2L_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1359155.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAZPEU010000597          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZPEU010000597 DAZPEU010000000
VERSION     DAZPEU010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.607"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>332
                     /locus_tag="ACHU2L_004668"
     CDS             <1..>332
                     /locus_tag="ACHU2L_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1359156.1"
                     /translation="QYLLSLVPYCPWQHIVFTLPCQYWSLVFHNRWLLAEMSRIAADV
                     ILEICHQADVEPGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMS
                     MWRYRITR"
BASE COUNT           71 a           88 c           95 g           78 t
ORIGIN      
        1 tacagtatct gctgagcctg gtcccctact gcccgtggca gcatattgtg ttcacacttc
       61 cctgccagta ctggtccctg gtgttccaca accggtggtt actggcagag atgagccgca
      121 ttgcagcgga tgtgatactg gaaatctgcc atcaggcaga tgtggagccg gggatattca
      181 cggtgatcca cacatggggg cgtgaccagc agtggcatcc gcatatccat ttatcgacaa
      241 ctgccggtgg tgtgacgtcg ggccacacct ggaaaaatct tcatttttac gcccgtaagg
      301 tgatgagcat gtggcgctac cggataacac gg
//
LOCUS       DAZPEU010000598          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZPEU010000598 DAZPEU010000000
VERSION     DAZPEU010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.608"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..329)
                     /locus_tag="ACHU2L_004669"
     CDS             complement(<1..329)
                     /locus_tag="ACHU2L_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR1359157.1"
                     /translation="MIANIIYSSASASTDISTVASPLFEGTEGCFLLYDASTNAEIAQ
                     FNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKTWM
                     QFSVVWVS"
BASE COUNT           92 a           60 c           65 g          113 t
ORIGIN      
        1 gaaacccaaa caacagaaaa ttgcatccac gtctttggtg tatgattgct gttccagatc
       61 tccattcctt tgggggtttt atcccatttg aatatggttt tctgatctat tatttccgca
      121 tcaaatgcca taagtgataa tgcgatcttg aaagttgaat ctggtgccat ttgcgttgca
      181 cactttgctt tattgaattg agcaatttca gcgtttgtgg atgcatcgta aagtaaaaaa
      241 caaccttcag ttccttcaaa taatggagat gcaacagtag agatatctgt tgatgcactg
      301 gcgctgctgt agataatatt tgcaattatt
//
LOCUS       DAZPEU010000599          329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZPEU010000599 DAZPEU010000000
VERSION     DAZPEU010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.609"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(41..99)
                     /locus_tag="ACHU2L_004670"
                     /pseudo
     CDS             complement(41..99)
                     /locus_tag="ACHU2L_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           74 a           96 c           76 g           83 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa ccaaccagta tgatgacgaa cttcatcata accctttcct tctataaggc
      121 cccttcctcg ggaggggctt tcccgtttca gccccttgct gactccccga ttgttgatga
      181 gcgccggacc ccgcgcaacg ctttccctga agcagttttg ccttccccga gcggtcacgc
      241 aaaacgctgc aacgtcctga gcatttgcac cttttagcgg aacgcagata taaaaaatcc
      301 ccggcttttt gggccaggga tatgtgtta
//
LOCUS       DAZPEU010000600          327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZPEU010000600 DAZPEU010000000
VERSION     DAZPEU010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.610"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          119 a           76 c           36 g           96 t
ORIGIN      
        1 tcatagggct tacagtttaa attgcgggta taaaatacat ctattttcaa ataaaataaa
       61 gcatactcca attacaacga gtgactaaaa taaaaatatc atccaagtca atcactgttt
      121 gctctgcaaa atatgtcagc cttctcaaac ttaccagcac ttaaatattt aagcacacaa
      181 tcattccttg agggagttca cttatttcaa gagaaacatt tcgtcttccc acacaactaa
      241 tccgcccaat atagctaaat ttgtactcag taatatcatt tgttatgccc cccaccccaa
      301 aatattcaag ctccttcaca gataaaa
//
LOCUS       DAZPEU010000601          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZPEU010000601 DAZPEU010000000
VERSION     DAZPEU010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.611"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            103..>325
                     /locus_tag="ACHU2L_004671"
     CDS             103..>325
                     /locus_tag="ACHU2L_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1359158.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          110 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttgaaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtg
//
LOCUS       DAZPEU010000602          323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZPEU010000602 DAZPEU010000000
VERSION     DAZPEU010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.612"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT           98 a           59 c           50 g          116 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga ggg
//
LOCUS       DAZPEU010000603          320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZPEU010000603 DAZPEU010000000
VERSION     DAZPEU010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.613"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>320)
                     /gene="rfbG"
                     /locus_tag="ACHU2L_004672"
     CDS             complement(<1..>320)
                     /gene="rfbG"
                     /locus_tag="ACHU2L_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFR1359159.1"
                     /translation="YSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFKAKYKRLGL
                     VRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKADKIYILLML
                     ILNI"
BASE COUNT           97 a           68 c           36 g          119 t
ORIGIN      
        1 tatgtttaaa atcaacatta ataaaatata aattttatca gcctttttcc gccttaactt
       61 ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt gattattcac
      121 aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat accacttgtt
      181 tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc ccacttggtt
      241 gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg aataatttct
      301 cgtccgcgca aatgaatata
//
LOCUS       DAZPEU010000604          320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZPEU010000604 DAZPEU010000000
VERSION     DAZPEU010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.614"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>320)
                     /locus_tag="ACHU2L_004673"
     CDS             complement(<1..>320)
                     /locus_tag="ACHU2L_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR1359160.1"
                     /translation="NGLTEAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIEN
                     MYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLT
                     SSIK"
BASE COUNT           78 a           57 c           56 g          129 t
ORIGIN      
        1 ttttatgctt gatgttaaat tcgaccccaa gtttcctgta agtgcggaca caaaaacata
       61 tttatgtcct gatttgctta taataaaccc ttcaaaccat ccgttttgta aggttctatt
      121 tgctgtgaat cctgcaccag ttttcccata cagttttgta ctattatcca gatcttgtag
      181 atacatgttc tctatggtgt tttctatggc tgagttttta actgggagat tgtgattaat
      241 aattttacgc aggaattgaa tttgttcttc tggtgaaatt tttaagctac tttcgagcca
      301 tgcttctgtt aatccgttgt
//
LOCUS       DAZPEU010000605          318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZPEU010000605 DAZPEU010000000
VERSION     DAZPEU010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.615"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>318)
                     /locus_tag="ACHU2L_004674"
     CDS             complement(<1..>318)
                     /locus_tag="ACHU2L_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFR1359161.1"
                     /translation="KSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDTVGSFFE
                     RFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNALYIYDSG
                     ELI"
BASE COUNT          112 a           57 c           40 g          109 t
ORIGIN      
        1 cttattaatt ctccagagtc atatatgtat aatgcatttc catctttata ccatatgagt
       61 cccacaagta atgtcagttt ttcaagcatt tcttctggat tagataaatc aaactcccca
      121 gatattctct tttttgcagc ttgtttgcta acaacaatag gataatttaa taacgcagaa
      181 aaacgttcaa aaaaagatcc taccgtatca ctttgagcaa catatttcgc tatgtcattt
      241 tgttctaata aatgagagtc aatattatta gcattaacta tcaatggtaa caatacaatc
      301 aagagagtca aagattta
//
LOCUS       DAZPEU010000606          313 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZPEU010000606 DAZPEU010000000
VERSION     DAZPEU010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.616"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..255)
                     /locus_tag="ACHU2L_004675"
     CDS             complement(<1..255)
                     /locus_tag="ACHU2L_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359162.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTY"
BASE COUNT          103 a           58 c           50 g          102 t
ORIGIN      
        1 atacgtatca aaactaagtg ctggcgctcc tagctttgag gcatcatagt taatgttaga
       61 catataggca atcatacgat tgaagttcga ctctgctatt tcatcgcagt cacattgacc
      121 cgttgatata aagaaagtga gtgccgccat ggtagactca agcctttctt taaaagagtc
      181 gaaagccagg cttttaatat ttaaatcacc ataattactc tgtattaagt ttgtagaaaa
      241 catctcccgc ctcatattgt taacaaaatt attatcttat ttaaatctaa gtcatttaca
      301 atataagttt aag
//
LOCUS       DAZPEU010000607          308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZPEU010000607 DAZPEU010000000
VERSION     DAZPEU010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.617"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>308
                     /locus_tag="ACHU2L_004676"
     CDS             <1..>308
                     /locus_tag="ACHU2L_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359163.1"
                     /translation="QGLFILSGENKIDEECFINIVQTAVAINNIGRESNGYILVGVSD
                     TKATADRVKTLYGVTPIECNGYYINGIDHEAVIQSKNIDNYFLFIKQKIESFNFNEAL
                     "
BASE COUNT          111 a           44 c           55 g           98 t
ORIGIN      
        1 cagggattat ttattctctc aggcgagaat aaaatagacg aagaatgttt cattaatata
       61 gtccaaacgg ctgtcgccat aaataacatc ggtagagaat ctaatggtta tattttagtt
      121 ggtgtttccg ataccaaagc tacggcagac agagttaaga ctttatatgg tgttacccct
      181 attgagtgca atggctatta tatcaatgga attgatcatg aagctgtaat tcaatcaaaa
      241 aacatagata attactttct gttcataaaa caaaaaatag aatcatttaa ttttaatgag
      301 gctttgaa
//
LOCUS       DAZPEU010000608          305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZPEU010000608 DAZPEU010000000
VERSION     DAZPEU010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.618"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT           97 a           78 c           49 g           81 t
ORIGIN      
        1 agtgcatgaa aaaagctttt ccctaccaat ccgggatata aataatcatc agtaccacaa
       61 ttattccaca gcaataaatc tcttcccgcc gctccggacc tctgataatg gtctaaaatc
      121 attgaggcca cttgcgacga cattgctcaa tgaggaaagt ttggcgtgaa taatattcaa
      181 caccagtgca gcatcatcat tacatactta tgcccgccaa ctaccgcttt cgatatattg
      241 actcaatttt tcagcctggt taaaaatagc gaccagctca gtcgcaaata tagtgactac
      301 cctaa
//
LOCUS       DAZPEU010000609          305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZPEU010000609 DAZPEU010000000
VERSION     DAZPEU010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.619"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT          103 a           64 c           49 g           89 t
ORIGIN      
        1 gttaatgaca ttaataacgt cgttcttaaa atcctcaaag tcttgttgcg gtaacttctg
       61 gttacgctgc ttactatcag caagcggtga atccggacga caacaacata ggttttgtat
      121 aacccccagc attcttttca cctccgttaa cagtatattc acgacactga aacgagagaa
      181 tgctgataat tttctcggcg agatatcaaa aatcgctcat tataaaacaa gtaacttcaa
      241 cgtcgcgtaa ccgtggacga taaaaaaaca ataatgtcat tcatatttaa ataatttagc
      301 cttaa
//
LOCUS       DAZPEU010000610          305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZPEU010000610 DAZPEU010000000
VERSION     DAZPEU010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.620"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="ACHU2L_004677"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="ACHU2L_004677"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR1359164.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT           96 a           54 c           82 g           73 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
      301 ttcta
//
LOCUS       DAZPEU010000611          304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZPEU010000611 DAZPEU010000000
VERSION     DAZPEU010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.621"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>304
                     /locus_tag="ACHU2L_004678"
     CDS             <1..>304
                     /locus_tag="ACHU2L_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1359165.1"
                     /translation="SAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAID
                     GVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSEN"
BASE COUNT           66 a          101 c           88 g           49 t
ORIGIN      
        1 agtgccgtta ttcaggcccg ccagcgcctg ggaagtgagg ctgtccgccg cgtgttcacg
       61 aaaacagcgc agctctggca taacgccacg ccgcatccgc actggtgcgg cctgaccctg
      121 ctggccatcg atggtgtgtt ctggcgcaca ccggatacac cagagaacga tgcagccttc
      181 ccccgccaga cacatgccgg gaacccggcg ctctacccgc aggtcaaaat ggtctgccag
      241 atggaactga ccagccatct gctgacggct gcagccttcg gcacgatgaa gaacagcgaa
      301 aatg
//
LOCUS       DAZPEU010000612          304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZPEU010000612 DAZPEU010000000
VERSION     DAZPEU010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.622"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>304)
                     /locus_tag="ACHU2L_004679"
     CDS             complement(<1..>304)
                     /locus_tag="ACHU2L_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1359166.1"
                     /translation="LFVIIAIDAIFREIVKNNIVILIISLLSFMLSQTVLNHNPLLDP
                     QWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSITAIMSAYQLLNQ"
BASE COUNT          107 a           52 c           53 g           92 t
ORIGIN      
        1 ttctggttaa gtagttgata cgcactcatt atagcggtaa ttgagaagac aatgaaacca
       61 attttagatt ttccaaaaaa cctgtcacgt gttaattcta aaaacataca tctaccgaga
      121 ggcaataacc accagtatgc catagcggag tctatgttcc aaaaccattg aggatctagt
      181 aatgggttat gattgagcac tgtttgtgac aacatgaacg ataacagtga tatgattaaa
      241 atcactatat tgttcttgac tatttctcta aagattgcat caattgctat tataacaaaa
      301 aggc
//
LOCUS       DAZPEU010000613          303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZPEU010000613 DAZPEU010000000
VERSION     DAZPEU010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.623"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..285
                     /locus_tag="ACHU2L_004680"
     CDS             <1..285
                     /locus_tag="ACHU2L_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1359167.1"
                     /translation="TIITTVGLIIFNIFIAKIICGNDFIVRAGRNTLNICGMEYITKL
                     FIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKIGHWLSRTVGGPFLIK"
BASE COUNT          100 a           47 c           55 g          101 t
ORIGIN      
        1 actattatca ccacggtagg cttgataatc tttaatattt tcatagcaaa aataatttgt
       61 ggaaatgact ttattgtaag agctggaaga aatactctta acatatgtgg aatggagtat
      121 atcaccaaat tatttatccc tatggcttta gcaattatag gcttcagcgt gactattcca
      181 aatcctatct gcgcaatcat atatacctgt atttgtgtat atgtatcaga taaaattggc
      241 cattggctat cgaggacagt cggtgggcca tttttgataa aatgataaaa aggcgcaatt
      301 aat
//
LOCUS       DAZPEU010000614          302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZPEU010000614 DAZPEU010000000
VERSION     DAZPEU010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.624"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            145..237
                     /locus_tag="ACHU2L_004681"
                     /pseudo
     CDS             145..237
                     /locus_tag="ACHU2L_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
BASE COUNT           99 a           43 c           52 g          108 t
ORIGIN      
        1 aaaatatttg catatacata taatcttaaa gtttagtcta tttaatgttc aatgaaatat
       61 ttctacctgt ataatcttta aagatgttga acatatattc acattaaata tgattatgta
      121 cttgttacaa ggataaggtt ataaatgaat aaagttacaa aaacagctat tgtgggctta
      181 cttgcgcttt tcgcaggtaa tgccgctgca accgatggtg aaatagtttt tgatggcggt
      241 aatgactcca acttactgat agtgttttat gttcagataa tgcccgatga ccttgtcatg
      301 ca
//
LOCUS       DAZPEU010000615          293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZPEU010000615 DAZPEU010000000
VERSION     DAZPEU010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.625"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            132..>293
                     /locus_tag="ACHU2L_004682"
     CDS             132..>293
                     /locus_tag="ACHU2L_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359168.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGIT"
BASE COUNT           90 a           57 c           56 g           90 t
ORIGIN      
        1 actgatatta ttcatcaaat aataacaaaa tagccattgc atcgggttgg acaatttaca
       61 aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata taaaatgatg
      121 gtcagattaa tatgcggatt atctttctac gcaaggagta tttatctcta ctcccgtcaa
      181 tgattgcatc tcttttctct gctaacggtg tcgcggcggc cattgattta tgccagggat
      241 atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt acg
//
LOCUS       DAZPEU010000616          291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZPEU010000616 DAZPEU010000000
VERSION     DAZPEU010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.626"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..180
                     /locus_tag="ACHU2L_004683"
     CDS             <1..180
                     /locus_tag="ACHU2L_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFR1359169.1"
                     /translation="AEQAIRLLASQGQSIRVRMGVCFVPKANVANSALFSDGAVETGT
                     ATVQSAKANTLPLRG"
BASE COUNT           81 a           64 c           77 g           69 t
ORIGIN      
        1 gcagagcaag caattcggtt gttggcaagt caagggcaaa gcattcgcgt gagaatgggc
       61 gtatgctttg tgccaaaagc aaacgttgct aattctgcac tgttcagcga tggcgcagta
      121 gagacaggca ctgccactgt ccagtcggca aaagcgaaca ctttgccact gcggggataa
      181 actgattgaa gctctcgcgc aaaatcactt attcttttgc gttaataaaa tgtaggagat
      241 gggtcattga tcctacccac gtaatatgga cacaggccta agcgaggttc t
//
LOCUS       DAZPEU010000617          290 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZPEU010000617 DAZPEU010000000
VERSION     DAZPEU010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.627"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            53..>290
                     /locus_tag="ACHU2L_004684"
     CDS             53..>290
                     /locus_tag="ACHU2L_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1359170.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQ"
BASE COUNT           92 a           50 c           57 g           91 t
ORIGIN      
        1 ataaagcgcg aggagttcgg tggattatcg tttttcttgg gtgattttta atatgataaa
       61 caatatatct gatcaagcat catcatttcc aggcacacaa ctaaatcaaa gtgataattt
      121 cctggattca ctgagggaat ttttcgcgat attaaatccc agtcgtaagg gtgagttgtc
      181 tacatgggat accatctact tgcatctgat attagcaata aatgctgaca gtgatctcat
      241 aaaaaatgat gtgttgctcg cggaaaatat tccatcagcg aattaccaat
//
LOCUS       DAZPEU010000618          289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZPEU010000618 DAZPEU010000000
VERSION     DAZPEU010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.628"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>289
                     /locus_tag="ACHU2L_004685"
     CDS             <1..>289
                     /locus_tag="ACHU2L_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1359171.1"
                     /translation="TLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKG
                     DHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           84 a           68 c           89 g           48 t
ORIGIN      
        1 gacgttaatg gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg
       61 agaacaccgc cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa
      121 actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg
      181 gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt
      241 ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggagga
//
LOCUS       DAZPEU010000619          286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZPEU010000619 DAZPEU010000000
VERSION     DAZPEU010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.629"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT           88 a           60 c           53 g           85 t
ORIGIN      
        1 agagtcataa aatagagtgt ctgttgatct cattttcata ttcctttcag actgaagtgc
       61 attttcatca cccgatgtga aagaactgaa gtgatgtttg ccatagatta cgagatgccc
      121 agtctcattg actatcgtgg tgcatcttta tacctgttat accagatcaa aaatcacgca
      181 atccatacaa caaaaccaga tttgcaattc gcgtcacaaa atatgtcgat ctttttctaa
      241 gaagaagatg ccatgtgaag ccagacgaac acttgcggtg gtcttc
//
LOCUS       DAZPEU010000620          285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZPEU010000620 DAZPEU010000000
VERSION     DAZPEU010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.630"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>285
                     /locus_tag="ACHU2L_004686"
     CDS             <1..>285
                     /locus_tag="ACHU2L_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359172.1"
                     /translation="HCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGARS
                     SFRSLEHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSN"
BASE COUNT           89 a           60 c           43 g           93 t
ORIGIN      
        1 cactgtgcta atactgtgaa aaacttttta aggaagtcta tagcagcgca aagttatagc
       61 aaaatgtttt ctcaagggac cagtttcaaa tcactaaatc tttctcttga agcgccatct
      121 ggagcacggt catcatttcg gtcacttgag catctcgata aggtgtccag gcactatatc
      181 tctgagataa tacaaaaagt tcatcctctt tcttcagacg aacgtcactt actttctatt
      241 attattaatt ctaattttaa cttcagacat caaagtaact ctaat
//
LOCUS       DAZPEU010000621          284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZPEU010000621 DAZPEU010000000
VERSION     DAZPEU010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.631"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            17..>284
                     /locus_tag="ACHU2L_004687"
     CDS             17..>284
                     /locus_tag="ACHU2L_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359173.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNQINNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLL"
BASE COUNT           72 a           64 c           59 g           89 t
ORIGIN      
        1 acgcagagga aacactatgc ataaatcaga agctgaaaaa atagattcta ttctggatga
       61 acatcaacag atatatcgca gacataacca aataaataac atcctggcgt attgtgcgtg
      121 tattgcatgg attccctgta ttattgctgc gttcggcgtt gatggtatat atctgaaaat
      181 cctgtttgcg gtccttgcct gctgtggtgc ggtatgcttt tttattttct tcttcaccct
      241 cctgccggaa tcactcatgg tcctcagccg tcagagcctg cttc
//
LOCUS       DAZPEU010000622          278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZPEU010000622 DAZPEU010000000
VERSION     DAZPEU010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.632"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            24..100
                     /locus_tag="ACHU2L_004688"
     tRNA            24..100
                     /locus_tag="ACHU2L_004688"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHU2L_004689"
     tRNA            143..218
                     /locus_tag="ACHU2L_004689"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZPEU010000623          275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZPEU010000623 DAZPEU010000000
VERSION     DAZPEU010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.633"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..227)
                     /locus_tag="ACHU2L_004690"
     CDS             complement(<1..227)
                     /locus_tag="ACHU2L_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR1359174.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATT"
BASE COUNT           88 a           51 c           47 g           89 t
ORIGIN      
        1 gtcgtcgcac caacaatgag tgttgatact gtttgaaaat aaccatttct tggggttatg
       61 agtgaactga aaaaaccttg ttcgtatgca tctttatacc aaaaaactgc atcctcagcc
      121 caaaactggg catttgtcaa aatgtcaatg cgtctaagcc aaatcaaagt aaatgcaagt
      181 ataaaaattg atatcttgtg ccaattgcgc attatgaata tcttcataag atcatcctgt
      241 tattttctgc ttttaattaa gtaccgtggt cttga
//
LOCUS       DAZPEU010000624          274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZPEU010000624 DAZPEU010000000
VERSION     DAZPEU010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.634"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            1..274
                     /gene="ydjO"
                     /locus_tag="ACHU2L_004691"
                     /pseudo
     CDS             1..274
                     /gene="ydjO"
                     /locus_tag="ACHU2L_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
BASE COUNT           78 a           47 c           55 g           94 t
ORIGIN      
        1 ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa gtttccttcg
       61 ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc gggaatggga
      121 agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa acgtctcggg
      181 tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg tattgataag
      241 ttggacattt ttttcgctga cgatctgttt aaat
//
LOCUS       DAZPEU010000625          270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZPEU010000625 DAZPEU010000000
VERSION     DAZPEU010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.635"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>270
                     /locus_tag="ACHU2L_004692"
     CDS             <1..>270
                     /locus_tag="ACHU2L_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1359175.1"
                     /translation="VWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLN"
BASE COUNT           68 a           69 c           75 g           58 t
ORIGIN      
        1 gtctggagac tgatgaaacg tgtcggaata aaggctcagg tcggataccg aagcccgcgg
       61 gcacgtaaag gcgaggccag tatcgtgtca cccaacaggc tccagcgaca gttcaatccg
      121 gatgctcctg atgagcgttg ggtaacggac ataacctaca tcaggaccca cgaaggctgg
      181 ctgtatcttg ccgttgttgt tgatctgttc tcacgcaaaa ttatcggctg gtccatgcaa
      241 tcccggatga caaaggacat tgtcctgaac
//
LOCUS       DAZPEU010000626          265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZPEU010000626 DAZPEU010000000
VERSION     DAZPEU010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.636"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..249
                     /gene="ospE1"
                     /locus_tag="ACHU2L_004693"
     CDS             <1..249
                     /gene="ospE1"
                     /locus_tag="ACHU2L_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HFR1359176.1"
                     /translation="FPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAAIYELQI
                     PAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
BASE COUNT           98 a           51 c           41 g           75 t
ORIGIN      
        1 ttcccttgtc ttccacaaaa acaagaaaat atcatccttg aagtatcaaa tcctgtatta
       61 ttatcttcta ctgttactac agatggatat actgttttca ataaaaaagc agctatatac
      121 gaattacaaa tacctgctgc cagccggacg aaaacgttga aattcactgc tacagaaatg
      181 caatggttga caaagataaa tgaagccgga attgatgaaa aacaaagcca acggtattct
      241 gatttctgaa gttaatttca gactc
//
LOCUS       DAZPEU010000627          261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZPEU010000627 DAZPEU010000000
VERSION     DAZPEU010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.637"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT           64 a           63 c           60 g           74 t
ORIGIN      
        1 gcgcggggtg tgcataatac gctttcccgc tacagagtca agcatttatt tttgctttct
       61 ctgccggaat tctcaggaga accccgctga cccggcggcg tgtttgccgt tgttccgtgt
      121 cagtggtggc gcattatagg gagttattcc ggcctgacaa gcgaaaaata taaaaacttt
      181 atcgttcgct cacttttcag gcaaaacatc ttaaatatag tcttttccgt ctaacttata
      241 gacaaaaacg agccccgaag g
//
LOCUS       DAZPEU010000628          258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZPEU010000628 DAZPEU010000000
VERSION     DAZPEU010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.638"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>258
                     /locus_tag="ACHU2L_004694"
     CDS             <1..>258
                     /locus_tag="ACHU2L_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR1359177.1"
                     /translation="GKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHD
                     VANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGEN"
BASE COUNT           67 a           71 c           71 g           49 t
ORIGIN      
        1 ggtaaggtgg aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact
       61 cgcctgcgcc cgatggggag cactgtcgat gttgaaacag ccaaccgcca cggtctgcgc
      121 tggctgcacg atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgtgat
      181 cgctggctcg aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac
      241 gtgcatcctg gtgaaaat
//
LOCUS       DAZPEU010000629          255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZPEU010000629 DAZPEU010000000
VERSION     DAZPEU010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.639"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>255
                     /locus_tag="ACHU2L_004695"
     CDS             <1..>255
                     /locus_tag="ACHU2L_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1359178.1"
                     /translation="SSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
                     EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRA"
BASE COUNT           70 a           62 c           74 g           49 t
ORIGIN      
        1 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
       61 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
      121 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
      181 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      241 ctggaaaaac cgtgc
//
LOCUS       DAZPEU010000630          253 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZPEU010000630 DAZPEU010000000
VERSION     DAZPEU010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.640"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>253
                     /locus_tag="ACHU2L_004696"
     CDS             <1..>253
                     /locus_tag="ACHU2L_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1359179.1"
                     /translation="GKDTHHAVAINRSGKRLFDKALPNDENKLRSLISDLKQHGQILL
                     VVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG"
BASE COUNT           63 a           73 c           59 g           58 t
ORIGIN      
        1 cggtaaagat acgcatcacg ctgttgccat taatcgttca ggtaaacgcc tgttcgataa
       61 agcattaccc aacgacgaaa acaaactcag gtcgctaata tctgacctga aacaacatgg
      121 tcagatactg ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc
      181 ccgctcagaa ggagtccttg tcggatacct ccctggactg gccatgcgcc gcatagccga
      241 cttacacgcc ggt
//
LOCUS       DAZPEU010000631          251 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZPEU010000631 DAZPEU010000000
VERSION     DAZPEU010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.641"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>251
                     /locus_tag="ACHU2L_004697"
     CDS             <1..>251
                     /locus_tag="ACHU2L_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1359180.1"
                     /translation="RQYACQVAGYEQEIERLKAQLDRLRRMLFGQSSEKKRHKLENQI
                     RQAEKRLSELENRLNTARNLLEDASSVTDSPDTSPPSEN"
BASE COUNT           78 a           62 c           65 g           46 t
ORIGIN      
        1 tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa attgaacggc tgaaagcgca
       61 actcgacagg ttgcgtcgta tgttgttcgg ccagagttca gagaaaaagc gtcataagct
      121 tgaaaatcag atccgacagg cagaaaaacg actgtcggaa ctggaaaacc ggctgaacac
      181 agccagaaat cttctggaag atgcatcgtc agtcacagat tcacctgaca ccagtccccc
      241 gtcagaaaac c
//
LOCUS       DAZPEU010000632          247 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZPEU010000632 DAZPEU010000000
VERSION     DAZPEU010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.642"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>247)
                     /locus_tag="ACHU2L_004698"
     CDS             complement(<1..>247)
                     /locus_tag="ACHU2L_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HFR1359181.1"
                     /translation="ILLNFGLLNFNLIYEHLSLTSEFFFRPDGAFFSKSFYFFGVGAI
                     ISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLLA"
BASE COUNT          106 a           40 c           36 g           65 t
ORIGIN      
        1 ctggctaata acagtgataa tgttgtaaaa agtaatacac ctcttgattc tgtcaataat
       61 atcgcaagca ctattatgag acattttaaa tattttttgt cgacaaaact gataatcgca
      121 ccgacaccaa aaaagtagaa ggatttggaa aaaaaagccc catcgggacg aaaaaagaac
      181 tcgctagtca atgataagtg ttcataaatt aaattaaaat ttaacaaacc gaaattcaac
      241 aaaatta
//
LOCUS       DAZPEU010000633          246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZPEU010000633 DAZPEU010000000
VERSION     DAZPEU010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.643"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT           83 a           46 c           55 g           62 t
ORIGIN      
        1 cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta gcacgtcgta
       61 tgcaagaacg tgccacggct ggctgatgga tgttcgatag cgcgagtttg aatgaaaatc
      121 agccggagat gattttacat aattgctacg gaattattca atacaggaat tgcttgcgtc
      181 tgcatggatt gacctgaaat attcccgaaa atttctctaa aaaactcgaa aaaaatggta
      241 actggt
//
LOCUS       DAZPEU010000634          244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZPEU010000634 DAZPEU010000000
VERSION     DAZPEU010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.644"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT           78 a           39 c           53 g           74 t
ORIGIN      
        1 tcgcatttaa tcgtttttat ctggatagcg ctcttttgat cggcaatgtt gaaataatga
       61 aagcggcata gattgagggg caataatttg aactcagtcg caaaattata attaaaacgg
      121 tcatgctacc taatatatgc ccgtttattg catcgcgaaa tttgagaaga cataactcat
      181 taaatgaaag ggatgcaaat gaatacgttt atcgctgctg aagctgcgga atgtataggc
      241 tgtc
//
LOCUS       DAZPEU010000635          244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZPEU010000635 DAZPEU010000000
VERSION     DAZPEU010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.645"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT           69 a           40 c           54 g           81 t
ORIGIN      
        1 tttattatgc tttaatgtgg ttgtccgatt aaggactaat ccttgaaaac gccgtatagg
       61 gcgagagttc actctattgc gataacgtaa ccgtacaata ttgtgcgttt cccaaattga
      121 ccactactga actattctgg gatttaaatg caataaaatg gttggttgaa ggtcgtggtg
      181 atgatgttag tccttttgag aatattccag tgccagatct tacttataat aaagaggctt
      241 ggca
//
LOCUS       DAZPEU010000636          243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZPEU010000636 DAZPEU010000000
VERSION     DAZPEU010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.646"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>243
                     /locus_tag="ACHU2L_004699"
     CDS             <1..>243
                     /locus_tag="ACHU2L_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR1359182.1"
                     /translation="RGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTMRNG
                     RSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNA"
BASE COUNT           50 a           65 c           66 g           62 t
ORIGIN      
        1 cgtggcggaa tgaccattct cagggcattc attcgttctc tctcggttcc tcaggagcag
       61 gagcctgccg ttcggttcga aactgaaccc ggacgacaga tgcaggttga ctggggcact
      121 atgcgtaatg gtcgctcacc gcttcacgtg ttcgttgctg ttcccggata cagccgaatg
      181 ttgtacatcg aattcactga caatatgcgt tatgacacgc tggagacctg ccatcgtaat
      241 gcg
//
LOCUS       DAZPEU010000637          243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZPEU010000637 DAZPEU010000000
VERSION     DAZPEU010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.647"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            101..>243
                     /locus_tag="ACHU2L_004700"
     CDS             101..>243
                     /locus_tag="ACHU2L_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFR1359183.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT           76 a           47 c           42 g           78 t
ORIGIN      
        1 taaatctgct atctctttat gtgactaact tcacttacat ccacttattt ctcttcgtaa
       61 aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg cattaaagaa
      121 aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg catttgctgc
      181 tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt tctcagactt
      241 tac
//
LOCUS       DAZPEU010000638          241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZPEU010000638 DAZPEU010000000
VERSION     DAZPEU010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.648"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
BASE COUNT           69 a           47 c           49 g           76 t
ORIGIN      
        1 tacgtcccgc ataaggcatt tttctcttta tctttgtacg gtacttcatg gaacagaatt
       61 tttgaccttg tgaatcgtga tatctgttgg ggaggggcaa tttgctcact gaagcgtgag
      121 actcgattaa gcgcacgaaa cacagaaatc aaaaaacccg gccacttttt tacaaagtgg
      181 ccgggtaaaa ataattttta ttttttaact gtcctgagtc tcatagagat gtctcaaaac
      241 t
//
LOCUS       DAZPEU010000639          241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZPEU010000639 DAZPEU010000000
VERSION     DAZPEU010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.649"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..233
                     /locus_tag="ACHU2L_004701"
     CDS             <1..233
                     /locus_tag="ACHU2L_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFR1359184.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT           63 a           43 c           65 g           70 t
ORIGIN      
        1 tgactaaata tttatttttt gaatcgcagg gcattccgga taaggttgat atcgtgtcac
       61 ggctgaagac ctatggatac agtatttctg gtgtggagac ggatgacgga tataaggcgc
      121 ttgtccgcgc ttttcagctt catttccggc agaaaaatta tgacggcata atggatgctg
      181 aaaccgctgc catcctgtat gcattgctgg agaagtattt cccgggtaaa taaggagtga
      241 g
//
LOCUS       DAZPEU010000640          238 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZPEU010000640 DAZPEU010000000
VERSION     DAZPEU010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.650"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..139)
                     /locus_tag="ACHU2L_004702"
     CDS             complement(<1..139)
                     /locus_tag="ACHU2L_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1359185.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NS"
BASE COUNT           69 a           42 c           44 g           83 t
ORIGIN      
        1 acgagtttgc gcagtactgc aaacgcggtg actcttctgg cattatttgg caatgcattg
       61 agcaactatt gcatgtaaga cttttataac gactacaaag ccccatgtta agaacctccc
      121 tatccgtata taattgcata atttcattgg tgattgatga tgtcaataca ccttgatttt
      181 gtaattttgc tgggaaaaat tgcgattaat ctgtatttgc tataattctg attaagat
//
LOCUS       DAZPEU010000641          237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZPEU010000641 DAZPEU010000000
VERSION     DAZPEU010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.651"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>237)
                     /locus_tag="ACHU2L_004703"
     CDS             complement(<1..>237)
                     /locus_tag="ACHU2L_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1359186.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSR"
BASE COUNT           44 a           80 c           50 g           63 t
ORIGIN      
        1 tcacgactca ggaactgttt caccatttgt gcatagcaca ctttcgccac tggttccggt
       61 ttatccatcc ccagagcacg gtacacctgc ggcagcttct ctccacacac ccggttggca
      121 aggaacccga agtacctcac catcttaaaa aacttctccg ggatgtgctg tttcagcctc
      181 gcgaccagct cacgctgtgt cagcgtttcc gtcgccgttt ctcccgtttt gtggtcc
//
LOCUS       DAZPEU010000642          233 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZPEU010000642 DAZPEU010000000
VERSION     DAZPEU010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.652"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>233
                     /locus_tag="ACHU2L_004704"
     CDS             <1..>233
                     /locus_tag="ACHU2L_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1359187.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASP"
BASE COUNT           71 a           39 c           71 g           52 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccg
//
LOCUS       DAZPEU010000643          233 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZPEU010000643 DAZPEU010000000
VERSION     DAZPEU010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.653"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..230
                     /locus_tag="ACHU2L_004705"
     CDS             <1..230
                     /locus_tag="ACHU2L_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1359188.1"
                     /translation="GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
                     EDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT           64 a           51 c           68 g           50 t
ORIGIN      
        1 tcggtgaaaa cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct
       61 cagacaaggg aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga
      121 acgaagtgga gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt
      181 cgaaccaagt gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tcc
//
LOCUS       DAZPEU010000644          232 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZPEU010000644 DAZPEU010000000
VERSION     DAZPEU010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.654"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>232
                     /locus_tag="ACHU2L_004706"
     CDS             <1..>232
                     /locus_tag="ACHU2L_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359189.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKAS"
BASE COUNT           79 a           42 c           47 g           64 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tc
//
LOCUS       DAZPEU010000645          231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZPEU010000645 DAZPEU010000000
VERSION     DAZPEU010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.655"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            22..>231
                     /locus_tag="ACHU2L_004707"
     CDS             22..>231
                     /locus_tag="ACHU2L_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR1359190.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKS"
BASE COUNT           54 a           61 c           64 g           52 t
ORIGIN      
        1 cggagtcgcc ccgcccttct gatgctcccc cgttttgccg atatttttca gcagggaaac
       61 cgctggctta actggctgga gaaacaaccg gaaggggcag tccgtccggt ggtaattgaa
      121 tcagtgacaa aaatcatggc ctgcgggacc acactgatgg gctacacgca gtggtgttgt
      181 tcatcaccgg actgttgcca tacaaaaaag gtctgcttcc ggtgtaaaag c
//
LOCUS       DAZPEU010000646          230 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZPEU010000646 DAZPEU010000000
VERSION     DAZPEU010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.656"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..>230)
                     /locus_tag="ACHU2L_004708"
     CDS             complement(<1..>230)
                     /locus_tag="ACHU2L_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1359191.1"
                     /translation="MSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYI
                     EMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT           56 a           59 c           42 g           73 t
ORIGIN      
        1 gacacttccg agccgttgat aatactggtt ttcatattct gtcggtgaca tctgttcgct
       61 agaaccatgc cgacgcttac tgttataaaa catttcgatg taatcaaaaa tatcactgcg
      121 ggcttcttcc cgcgttccgt agatcttttt ctttatccgt tcacgtttca acaactggaa
      181 aaaactttct gcaaccgcat tatcatggca gttaccgcga cggctcatgc
//
LOCUS       DAZPEU010000647          229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZPEU010000647 DAZPEU010000000
VERSION     DAZPEU010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.657"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..>229
                     /locus_tag="ACHU2L_004709"
     CDS             <1..>229
                     /locus_tag="ACHU2L_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1359192.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHWNAL"
BASE COUNT           69 a           48 c           71 g           41 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctg
//
LOCUS       DAZPEU010000648          226 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZPEU010000648 DAZPEU010000000
VERSION     DAZPEU010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.658"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..212)
                     /locus_tag="ACHU2L_004710"
     CDS             complement(<1..212)
                     /locus_tag="ACHU2L_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359193.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT           60 a           52 c           37 g           77 t
ORIGIN      
        1 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
       61 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acattattga ttttggggtt
      121 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
      181 ttgctactaa atagtttctt tattttttcc acgatgcccc caggtt
//
LOCUS       DAZPEU010000649          224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZPEU010000649 DAZPEU010000000
VERSION     DAZPEU010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.denovo.659"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            26..181
                     /locus_tag="ACHU2L_004711"
                     /pseudo
     CDS             26..181
                     /locus_tag="ACHU2L_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT           74 a           50 c           52 g           48 t
ORIGIN      
        1 ctccaacaac ggagacaggt aaattatgga cgaaaagaag cttaaagccc ttgcggctga
       61 actggctaaa ggcctcaaaa ccgaggccga cctcaatcag ttttcccgta tgcagacgaa
      121 attaactgtc gaaacggtgc ttaatgcgga actaactgac catctcgggc atgaaaaaag
      181 ctacatccgg tagtaaacat tatcagtggg ttatgcgata tgat
//
LOCUS       DAZPEU010000650        15041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.guided.088, whole genome shotgun sequence.
ACCESSION   DAZPEU010000650 DAZPEU010000000
VERSION     DAZPEU010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.guided.088"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            110..1015
                     /locus_tag="ACHU2L_004712"
     CDS             110..1015
                     /locus_tag="ACHU2L_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR1359194.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACHU2L_004713"
                     /gene_synonym="mrx"
     CDS             1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACHU2L_004713"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR1359195.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACHU2L_004714"
     CDS             2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACHU2L_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR1359196.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3327..4091)
                     /locus_tag="ACHU2L_004715"
     CDS             complement(3327..4091)
                     /locus_tag="ACHU2L_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR1359197.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4318..4623)
                     /locus_tag="ACHU2L_004716"
     CDS             complement(4318..4623)
                     /locus_tag="ACHU2L_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR1359198.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="ACHU2L_004717"
     CDS             complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="ACHU2L_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR1359199.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            complement(5995..6198)
                     /locus_tag="ACHU2L_004718"
     CDS             complement(5995..6198)
                     /locus_tag="ACHU2L_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359200.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="ACHU2L_004719"
     CDS             complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="ACHU2L_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR1359201.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(7159..7494)
                     /locus_tag="ACHU2L_004720"
     CDS             complement(7159..7494)
                     /locus_tag="ACHU2L_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HFR1359202.1"
                     /translation="MRYPRNAVLFSLFADNVRRTEGFTKLAPSAVVIIGYGIAFYFLS
                     LVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSPSW
                     KSLRRPTPW"
     gene            7387..7752
                     /gene="hypA"
                     /locus_tag="ACHU2L_004721"
     CDS             7387..7752
                     /gene="hypA"
                     /locus_tag="ACHU2L_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1359203.1"
                     /translation="MTDYDNGGRGKLSKALGAADIIREQAEQHGIARVTDVWLEVGAL
                     ADVEESALHFCFDIACRDTVAQGCTLHIDVIPAQAWCWDCSREAEIMQHAGCCPHCGS
                     ERLRISEGDDLRVKSLEGE"
     gene            complement(7756..8631)
                     /gene="ampR"
                     /locus_tag="ACHU2L_004722"
     CDS             complement(7756..8631)
                     /gene="ampR"
                     /locus_tag="ACHU2L_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator AmpR"
                     /protein_id="HFR1359204.1"
                     /translation="MVRRYLPLNPLRAFEAAARHLSFTRAAIELNVTHAAVSQQVRAL
                     EEQLGCVLFTRVSRGLVLTHEGEGLLPVLNEAFDRIADTLECFSHGQFRERVKVGAVG
                     TFAAGWLLPRLAGFYDSHPHIDLHISTHNNHVDPAAEGHDYTIRFGNGAWHESDAELI
                     FSAPHAPLCSPAIAEQLQQPDDVHRFTLLRSFRRDEWSRWLDCAGGTPPSPSQPVMVF
                     DTSLAMAEAAQLGAGVAIAPVCMFSRLLQSGALVQPFAAEITLGGYWLTRLQSRTETP
                     AMQQFARWLLNTAAA"
     gene            8742..9881
                     /locus_tag="ACHU2L_004723"
     CDS             8742..9881
                     /locus_tag="ACHU2L_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032097955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class C beta-lactamase DHA-1"
                     /protein_id="HFR1359205.1"
                     /translation="MKKSLSATLISALLAFSAPGFSAADNVAAVVDSTIKPLMAQQDI
                     PGMAVAVSVKGKPYYFNYGFADIQAKQPVTENTLFELGSVSKTFTGVLGAVSVAKKEM
                     ALNDPAAKYQPELALPQWKGITLLDLATYTAGGLPLQVPDAVKSRADLLNFYQQWQPS
                     RKPGDMRLYANSSIGLFGALTANAAGMPYEQLLTARILAPLGLSHTFITVPESAQSQY
                     AYGYKNKKPVRVSPGQLDAESYGVKSASKDMLRWAEMNMEPSRAGNADLEMAMYLAQT
                     RYYKTAAINQGLGWEMYDWPQQKDMIINGVTNEVALQPHPVTDNQVQPYNRASWVHKT
                     GATTGFGAYVAFIPEKQVAIVILANKNYPNTERVKAAQAILSALE"
     gene            10076..10897
                     /locus_tag="ACHU2L_004724"
     CDS             10076..10897
                     /locus_tag="ACHU2L_004724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1359206.1"
                     /translation="MDVLRFILRLPFILLRLAARSLVYLFTLLGFLLRPFTGRIRWAV
                     PGWVTFAGNQLARLERGGNRYPKTISALLLLTAAVAAGSYYTWHWYQNKPKPVDVAPL
                     VVQDISASVQRPSAVNYNRDDNSAQIVVVTFSRSAAPVTLIGKPVTAGITLTPAMEGE
                     WQWRNDRKLVFTAKKTFPMGKTYTVDMDAKTLLAPQVALTEKQKTFTTPEFYYRGGRA
                     EFYQDPQDPMKKHAIIGLTFNAPADVKNLESRLSMTRDGKPVPYTVTVMNCCHLC"
     gene            10855..11043
                     /locus_tag="ACHU2L_004725"
                     /pseudo
     CDS             10855..11043
                     /locus_tag="ACHU2L_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
     gene            complement(11232..11462)
                     /gene="pspD"
                     /locus_tag="ACHU2L_004726"
     CDS             complement(11232..11462)
                     /gene="pspD"
                     /locus_tag="ACHU2L_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1359207.1"
                     /translation="MNNRWQRAGQRVKPGIKIAGKLVLLTALRYGPAGVAGWAVKSVA
                     RRPLKMLLALVLEPLLSRAATKLSKRYSGNQP"
     gene            complement(11471..11830)
                     /gene="pspC"
                     /locus_tag="ACHU2L_004727"
     CDS             complement(11471..11830)
                     /gene="pspC"
                     /locus_tag="ACHU2L_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1359208.1"
                     /translation="MGGVNLNKKLWRIPQQGMVKGVCAGIAHYLDVPVKLVRILVVLS
                     IFFGLAFFTFVAYIVLTFVLDPMPDNVVSGEQQPSSGELLDAVDRELAASEKRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(11830..12054)
                     /gene="pspB"
                     /locus_tag="ACHU2L_004728"
     CDS             complement(11830..12054)
                     /gene="pspB"
                     /locus_tag="ACHU2L_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1359209.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSSRGELAQSEQQRLV
                     ELNQDAQRMRERIQALEDILDAEHPNWRDR"
     gene            complement(12109..12777)
                     /gene="pspA"
                     /locus_tag="ACHU2L_004729"
     CDS             complement(12109..12777)
                     /gene="pspA"
                     /locus_tag="ACHU2L_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1359210.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     SNSARALAEKKQLSRRIEQASAQQAEWQEKAELALRKEKEDLARSALIEKQKLTDLIA
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAASSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHNFGKQKSLDQQFADLKADDEISEQLAQLKAKMQ
                     QDKQ"
     gene            12933..13922
                     /gene="pspF"
                     /locus_tag="ACHU2L_004730"
     CDS             12933..13922
                     /gene="pspF"
                     /locus_tag="ACHU2L_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1359211.1"
                     /translation="MVNFIMAGYKDNLLGEANSFIEVLEQVSRLAPLDKPVLVIGERG
                     TGKELIANRLHYLSTRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFE
                     RADGGTLFLDELATAPMLVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPRM
                     VSEGTFRADLLDRLAFDVVQLPPLRERQSDIMLMAEHFAIQMCREIGLPLFPGFTDEA
                     KETLLHYRWPGNIRELKNVVERSVYRHGTSDYPLNEIVIDPFRRHAAPPLAQETRHTS
                     VTLPLDLREFQQQQEKEFLQTSLQQAKFNQKKAAELLGLTYHQLRALLKKHQI"
     gene            14005..14649
                     /locus_tag="ACHU2L_004731"
     CDS             14005..14649
                     /locus_tag="ACHU2L_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB4"
                     /protein_id="HFR1359212.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQ
                     FYDRESQKGCNFSRANLKDAIFKSCDLSMADFRNINALGIEIRHCRAQGSDFRGASFM
                     NMITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGE
                     FSSFDWRAANVTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLLERLGIAVMG"
     gene            complement(14745..>15041)
                     /locus_tag="ACHU2L_004732"
     CDS             complement(14745..>15041)
                     /locus_tag="ACHU2L_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1359213.1"
                     /translation="RQKAASSLTLQQCPKSLAKQLAPKGIRVNAVAPGPYWTVLQCCG
                     GQPQEKIEKFGANAPLGRPGQPVEIAPLYVTLAAEENSYTSGQVWCSDGGTGTL"
BASE COUNT         3294 a         4267 c         4200 g         3280 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     3421 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     3481 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     3541 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     3601 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     3661 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     3721 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     3781 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     3841 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     3901 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     3961 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4021 tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg gcgccacttg
     4081 aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct tcacgatttt
     4141 tgcaacagag cccacacgag tattgagcat agtcgagatt ggtgcagatc acttctgata
     4201 ttgaactgtc aggagctggc tgcacaacag ccattacgcc caatcaactg gtgcagtcgt
     4261 cttctgaaaa tgacaatcca gttagggtat agctcaacct gacatagaag caaaaactca
     4321 accaccttct accaactctc cgaacagctc cttgaccttt gttttcgcat cagcaagtgc
     4381 agttctgcct tgttcagtga tgtcataaac acgccgttca cgtcgcccgg tgcgttcgtg
     4441 gcgtgaggtc agatagcctt ttttttccag gccgtgcagc atcgggtaca cggtgccagc
     4501 gctcatctcg tagccgtgtc ggcgtagctc ttcgatgatc cccagcccaa agacaggttc
     4561 ctcggctgca tggtgaagga tgtgcaggcg gatcaaaccg ccgtagaggt ctttgtcagt
     4621 cattttttgt gcctcacaga gcgacgctca acagccaccc agctgcaccg ctaccgagga
     4681 caaccagcca cggcgggagc ttccagaaca taagtgcgac aagggcaact aatgccaagc
     4741 cgaagtcttg cggctgaaag atggcgctag tccatacagg ctgatacagc gcggccagca
     4801 gcaagccgac tacagcggca ttgatcccgg ccagcgcagc ttggatgcct gtattgcggc
     4861 gcaaacgctc ccaaaatggc attgatccga cgaccagcaa gaacgagggc gcgaagatag
     4921 ccagcagaca cacaatgccg ccgatccagc ccgacggggc ggtgttcatc gaggcaccaa
     4981 gaaacgcggc gaacgtgaac aaagggccgg gcaccgcttg agctgccccg taccccgcga
     5041 gaaaggattc attgttgacc cagccggagg gcaccacttc ggcttgcagt aatggcagca
     5101 caacgtgacc accgccgaac accagtgatc cgacacgata gaaggaatcc accattgcca
     5161 tggtttgact tggcatcagt tcggccaaca ccggcaggcc aatcagcaag acaaagaaca
     5221 gcgagagcca aagcacgccg gcccggtgac tgaccgtgat aggtaggggg tcatgctcaa
     5281 caactttcgc tggcttgaac aataaccggc ctgcgatgcc tgcgatagca atcacgccaa
     5341 cctgtcccca cgcggacggc acaagtaaaa cgacgcaggt agcaattgcc atgatggtga
     5401 ctcgcagccc atccgtgcat aggttacgcg ccatgcccca tactgcttga gcgaccacgg
     5461 ccacagccac cacttttaag ccatgcaacg cgccctgcga gacgtaatcg ccatagctgg
     5521 agatgccgag cgcaaaaagg atcaaggcta tggcagacgg cagcgtgaag ccagcccaag
     5581 cagccagcgc cccgctgtat ccagcccgag acagtcctac cgctatgccg acctggctgc
     5641 ttgcaggccc tggcaagaac tgacaaagcg cgaccaagtc agcatagctc cgttcggaga
     5701 gccagcgccg ccgtgtgaca aattcggcgc ggaagtagcc caagtgcgca atggggccgc
     5761 caaaagatgt caatccaagc cgcagaaaaa taagaaagac cgaccatggt ctgctgtcat
     5821 cggtagggtt attcgtcata ctttcgcctt catgatctgc aacgagttga tcaataataa
     5881 gcgaaattcg ataacgaaat tcgatataaa tctagaaaaa aatacctcta tgtgtactac
     5941 gcagttttag ctgtggcttt cacaggagca cgcttactta cggcttagcg tgctttattt
     6001 tccgttttct gaggcgatcc ctaggagctc ggatctcagg acgaaggtct ccgcgaatgt
     6061 ccggtcgatc cgcgcgacgt cccaggcggg cgttcccttg gcggacatcc acgccgcagc
     6121 gtcgtgcatc agccgcacaa cctcgtcgat atcacccgag caggcgaccc gaacgttcgg
     6181 aggctcctcg ctgtccattc gctcccctgg cgcggtatga accgccgcct catagtgcag
     6241 tttgatcctg acgagcccag catgtctgcg cccaccttcg cggaacctga ccagggtccg
     6301 ctagcgggcg gccggaaggt gaatgctagg catgatctaa ccctcggtct ctggcgtcgc
     6361 gactgcgaaa tttcgcgagg gtttccgaga aggtgattgc gcttcgcaga tctccaggcg
     6421 cgtgggtgcg gacgtagtca gcgccattgc cgatcgcgtg aagttccgcc gcaaggctcg
     6481 ctggacccag atcctttaca ggaaggccaa cggtggcgcc caagaaggat ttccgcgaca
     6541 ccgagaccaa tagcggaagc cccaacgccg acttcagctt ttgaaggttc gacagcacgt
     6601 gcagcgatgt ttccggtgcg gggctcaaga aaaatcccat ccccggatcg aggatgagcc
     6661 ggtcggcagc gaccccgctc cgtcgcaagg cggaaacccg cgcctcgaag aaccgcacaa
     6721 tctcgtcgag cgcgtcttcg ggtcgaaggt gaccggtgcg ggtggcgatg ccatcccgct
     6781 gcgctgagtg cataaccacc agcctgcagt ccgcctcagc aatatcggga tagagcgcag
     6841 ggtcaggaaa tccttggata tcgttcaggt agcccacgcc gcgcttgagc gcatagcgct
     6901 gggtttccgg ttggaagctg tcgattgaaa cacggtgcat ctgatcggac agggcgtcta
     6961 agagcggcgc aatacgtctg atctcatcgg ccggcgatac aggcctcgcg tccggatggc
     7021 tggcggccgg tccgacatcc acgacgtctg atccgactcg cagcatttcg atcgccgcgg
     7081 tgacagcgcc ggcggggtct agccgccggc tctcatcgaa gaaggagtcc tcggtgagat
     7141 tcagaatgcc gaacaccgtc accatggcgt cggcctccgc agcgacttcc acgatgggga
     7201 tcgggcgagc aaaaaggcag caattatgag ccccatacct acaaagcccc acgcatcaag
     7261 cttttgccca tgaagcaacc aggcaatggc tgtaattatg acgacgccga gtcccgacca
     7321 gactgcataa gcaacaccga cagggatgga tttcagaacc agagaaagaa aataaaatgc
     7381 gatgccataa ccgattatga caacggcgga aggggcaagc ttagtaaagc cctcggtgcg
     7441 gcggacatta tccgcgaaca ggctgaacag cacggcattg cgcgggtaac ggatgtctgg
     7501 ctggaagtcg gcgcactggc ggatgttgag gagagtgcac tgcatttctg ttttgatatc
     7561 gcctgccgtg ataccgtggc gcagggctgc acactgcata ttgatgttat cccggcacag
     7621 gcatggtgct gggattgcag ccgtgaggcc gaaatcatgc agcacgccgg atgctgtccg
     7681 cactgcggca gtgaacggct gcgcatcagt gaaggtgatg atttgcgggt aaaaagcctg
     7741 gaaggtgagt gagttttacg ccgccgccgt attcagcagc cagcgggcga attgctgcat
     7801 ggccggggtt tccgtacggg actgtaaccg cgtcagccag tagccgccga gggtgatttc
     7861 tgcggcaaac ggctgtacca gtgcgcctga ctgtaacagg cggctgaaca tacataccgg
     7921 tgcgatcgct accccggcac ccagttgtgc cgcctcggcc atggccagtg aggtatcgaa
     7981 caccattacc ggctgtgacg gggaaggcgg tgtgccgccc gcacaatcca gccagcggct
     8041 ccattcatcc cggcggaatg agcgcagcag ggtaaagcgg tgaacatcat ccggctgctg
     8101 taactgttct gcaatggccg gtgagcacag cggagcgtgt ggtgcactga aaatcagttc
     8161 cgcatctgac tcatgccacg cgccgttacc gaaacggatc gtataatcat gcccttccgc
     8221 cgccgggtcc acatgattgt tatgggtgga gatatgcaga tcaatatgcg gatggctgtc
     8281 atagaatccg gccagacgcg gcagcagcca gcctgcggca aatgttccca ccgcaccgac
     8341 tttcacccgc tcacggaact gcccgtgaga aaaacactcc agagtatccg caatccggtc
     8401 aaacgcctca ttgagcaccg gcagtaatcc ctcaccttca tgggtcagca ccagcccgcg
     8461 cgagacgcgg gtaaacagca cacagccgag ttgttcttcc agcgccctga cctgctggct
     8521 gacggcggca tgggtgacat tcagctcaat cgccgcgcgg gtaaaactga gatgacgggc
     8581 ggcggcctca aaggcgcgca gcgggttaag ggggagataa cgtctgacca taatccacct
     8641 gtaagttttt ctttaggctc ttgttataaa taaccgtttg ttctgtccgg tgaatctgac
     8701 gataattgcc gccgttactc acacacggaa ggttaattct gatgaaaaaa tcgttatctg
     8761 caacactgat ttccgctctg ctggcgtttt ccgccccggg gttttctgcc gctgataatg
     8821 tcgcggcggt ggtggacagc accattaaac cgctgatggc acagcaggat attcccggga
     8881 tggcggttgc cgtctccgta aagggtaagc cctattattt caattatggt tttgccgata
     8941 ttcaggcaaa acagccggtc actgaaaata cactatttga gctcggatct gtaagtaaaa
     9001 ctttcacagg tgtgctgggt gcggtttctg tggcgaaaaa agagatggcg ctgaatgatc
     9061 cggcggcaaa ataccagccg gagctggctc tgccgcagtg gaaggggatc acattgctgg
     9121 atctggctac ctataccgca ggcggactgc cgttacaggt gccggatgcg gtaaaaagcc
     9181 gtgcggatct gctgaatttc tatcagcagt ggcagccgtc ccggaaaccg ggcgatatgc
     9241 gtctgtatgc aaacagcagt atcggcctgt ttggtgctct gaccgcaaac gcggcgggga
     9301 tgccgtatga gcagttgctg actgcacgga tcctggcacc gctggggtta tctcacacct
     9361 ttattactgt gccggaaagt gcgcaaagcc agtatgcgta cggttataaa aacaaaaaac
     9421 cggtccgcgt gtcgccggga cagcttgatg cggaatctta cggcgtgaaa tccgcctcaa
     9481 aagatatgct gcgctgggcg gaaatgaata tggagccgtc acgggccggt aatgcggatc
     9541 tggaaatggc aatgtatctc gcccagaccc gctactataa aaccgccgcg attaaccagg
     9601 ggctgggctg ggaaatgtat gactggccgc agcagaaaga tatgatcatt aacggtgtga
     9661 ccaacgaggt cgcattgcag ccgcatccgg taacagacaa ccaggttcag ccgtataacc
     9721 gtgcttcctg ggtgcataaa acgggcgcaa caactggttt cggcgcctat gtcgccttta
     9781 ttccggaaaa acaggtggcg attgtgattc tggcgaataa aaactacccg aataccgaaa
     9841 gagtcaaagc tgcacaggct attttgagtg cactggaata atttttttcc tgaacaggcc
     9901 cctgcggata cgccggggcc gtaatttaag attattcctt atgcaataat ttgctttatg
     9961 ggctgtttta tggaggatga gagtatcggg cactgacagc ttccggctga accggatttc
    10021 actgtgtgct gcggcaaact gatgtcagtc tcaacacaca gaggaaaatg ttgtcatgga
    10081 cgttctgcgt tttatcctcc gcttaccttt tattttgctg cgtctggcgg ctcgcagtct
    10141 tgtttatctc tttactctgc tgggttttct gctgcgcccg ttcaccggac ggatccgctg
    10201 ggcggtgccg ggctgggtga cctttgccgg taatcagctt gcccggctgg agcggggggg
    10261 caaccgctat ccgaaaacca tatctgcctt attattactg actgctgcgg tggcagcggg
    10321 cagttattac acctggcact ggtatcagaa caaaccgaag ccggtggatg ttgcgccgct
    10381 ggtggtgcag gatatttccg cgtctgtgca gcgcccttcg gcggttaatt ataaccgtga
    10441 tgataattca gcgcagattg tggtggtgac attctcccgc tctgccgcac cggtaacgct
    10501 gatcggtaaa ccggtcaccg ccggtattac cctgaccccc gcaatggaag gtgagtggca
    10561 gtggcgtaat gaccgtaagc tggtattcac ggcgaaaaaa accttcccga tggggaaaac
    10621 ctacaccgtc gatatggatg ccaaaacact gctggctccg caggtggcgt taacagagaa
    10681 acaaaaaacc tttaccaccc ctgagtttta ttatcgcggc ggccgggcgg agttctatca
    10741 ggatccgcaa gatccgatga aaaaacacgc cattatcggc ctgaccttca atgcccccgc
    10801 tgatgtcaaa aatctggaat cgcgtctgtc tatgacccgt gacggcaagc cggtgccgta
    10861 taccgtcact gtaatgaact gttgtcattt atgctgacgc ccgtgccggg aggcattgat
    10921 gtcccaaccc agcagctcgg cgaacaaagc tgtaatttat tatttagcct gattgccggt
    10981 gagaaaggtt tacaaaatat caccgtgggt acacaaatat cgttagccga ctcactggta
    11041 taaaaactgg ggcataacaa tgccccagtg cattttagaa tgcgtaattc acaccgatac
    11101 ctgcataatg gagtctgtcg ctttcacctt catttctatt ggtagcgatc ccatgacaca
    11161 agagactact ctgccgcaaa ttaactgggg gaatgttgac taaatgttgc ttgcttttgt
    11221 tgtttcggat atcagggttg attacctgaa tagcgtttcg acaattttgt cgctgcccgg
    11281 ctcaacaaag gttccaatac taacgccagc aacattttta gcggacgtcg ggccacggac
    11341 ttcaccgccc accctgctac cccagccggg ccatagcgca gcgcagtcag tagcaccagc
    11401 ttacctgcta ttttaatgcc cggcttaacc ctttgcccgg cacgttgcca gcgattattc
    11461 atttctaacc tcacaattgg cggaagcggc tgcgtaatgt aaaagtatca gacgttacat
    11521 aacgctccat ctcacgcaaa cgcttttcac ttgcagccag ttcacgatcg acagcatcca
    11581 atagctcacc gcttgatggc tgctgttctc cggaaaccac gttgtctggc atcggatcca
    11641 acacaaacgt cagtacgatg taggcgacaa aggtgaaaaa ggccagacca aaaaagatgg
    11701 acagcaccac caggatccgc accagtttca caggaacatc gagataatgc gcaatacccg
    11761 cgcaaacccc tttcaccatg ccctgctgcg gtatacgcca cagtttttta ttcagattaa
    11821 cgccccccat tagcggtccc tccagtttgg atgttcagca tcaaggatgt cttccagcgc
    11881 ttgaatgcgc tcacgcatcc gctgtgcgtc ctggttaagt tctaccagac gctgttgttc
    11941 actttgcgcc agctcgccgc gactggaacg attgctgtaa tgcagccaga gccaaatcgg
    12001 taagacgaac agcacaaaga tggttaacgg gatggccaga aatagcgcgc tcatgagtac
    12061 tccttacagg tgatgagcgg cggcgcgttc gggcaccgcc agatattatt attgcttgtc
    12121 ttgctgcatt ttggctttca attgcgccaa ctgctcgctg atttcatcat ctgctttcag
    12181 gtcagcaaat tgctgatcca aagacttctg cttaccgaag ttatggcttt cagcttcagc
    12241 ttccatttga tcgatacgac gctcaaaaga ttcaaagcga gccatcgctt catccagttt
    12301 gccgctgtcc agttgacgac gaacatcacg ggaagaactc gccgcctgat ggcgcaacat
    12361 caatgcctgc tgacgagcac gggtttcact gagtttgttt tccagttcac cgatctcttt
    12421 tttcatacgc gccagcgtat cgtcaaccag cgtcacctca tgctccaggg aggcaatcag
    12481 gtcagtcagt ttctgctttt cgattaacgc agaacgcgcc aaatcttctt tttctttacg
    12541 cagcgccagc tctgcttttt cctgccactc tgcctgttgg gcgctggcct gttcaatacg
    12601 acgcgataac tgtttctttt ctgccagcgc gcgtgcggag ttggagcgca cctcaaccag
    12661 cgtgtcttcc atttcctgga tcatcaaccg caccagcttc tgcggatctt cagccttttc
    12721 cagcaacgca ttaatgttgg cgttcacgat gtcggcaaaa cgagaaaaaa tacccataat
    12781 gtaatcctca cttactgttc tgatatcggg caatgccctg ctgctgttct aatgcacttc
    12841 gcatgccaac tttttaattc attgaaatat ttgaaaataa ctaattttac ctgtcgggat
    12901 aaatagggta aattggtgaa taacactaac aaatggtgaa tttcatcatg gcaggataca
    12961 aagataatct gctgggcgaa gcgaacagct ttattgaagt gctggagcag gtttctcgtc
    13021 tcgcgccgct ggataaacct gtgctggtca ttggggaacg aggaacaggg aaagagctta
    13081 tcgccaaccg cctgcactac ctctcaaccc gttggcaagg cccgtttatt tcacttaact
    13141 gcgccgcgct caacgaaaat ttacttgatt ccgaactctt tgggcacgaa gcgggcgcct
    13201 ttaccggcgc gcagaaacgt cacccaggtc ggttcgagcg cgccgatggc ggcacactgt
    13261 ttcttgatga actggcaacc gccccgatgc tggtacagga aaaactgctg cgcgttattg
    13321 agtacggaga gctggaacgc gtaggcggta gccaacctct gcaggtcaat gtaagactgg
    13381 tttgcgcaac caacgccgac ctgccacgca tggtgagtga aggcacgttc cgcgccgacc
    13441 tgctggatcg tctggccttt gacgtggtgc aacttccccc gttacgcgaa cgtcaaagcg
    13501 atatcatgtt aatggctgaa cattttgcca ttcagatgtg ccgtgagatc gggttaccgt
    13561 tgtttcctgg gttcaccgat gaggccaaag aaacgctcct ccactaccgt tggccgggca
    13621 atattcgtga actgaaaaac gtcgtggagc gttcggtata ccgacacggg accagcgatt
    13681 acccactgaa tgagattgtg attgacccgt tcaggcgtca cgccgcaccg ccgctggcgc
    13741 aagaaaccag acatacatca gtaaccctac cgctggatct gcgtgaattc caacagcagc
    13801 aggagaagga atttttgcaa accagcttac agcaggcgaa atttaatcag aaaaaagccg
    13861 ctgaattatt aggtctgacc taccatcaac tccgcgcatt actgaagaaa caccaaattt
    13921 agcgtgcagg ggcagacgtt ggacgcgaga gttaaaacag tttaccatgt tgcaatcact
    13981 gtataaaaaa acaggttaat catgatgact ctggcgttag ttggcgaaaa aattgacaga
    14041 aacaggttca ccggtgaaaa agttgaaaat agcacatttt tcaactgtga tttttcgggt
    14101 gccgacctta gcggcactga atttattggc tgccagtttt atgatcgaga aagtcagaaa
    14161 ggatgtaatt ttagtcgcgc taacctgaaa gatgccattt tcaaaagttg tgatctctcc
    14221 atggctgatt tcaggaatat caatgcgctg ggaatcgaaa ttcgccactg ccgggcacaa
    14281 gggtcagatt ttcgcggcgc aagttttatg aatatgatca ccacccgcac ctggttttgt
    14341 agcgcctata tcaccaatac caacttaagc tacgccaact tttcaaaagt cgtactggaa
    14401 aagtgcgagc tgtgggaaaa ccgctggatg ggtactcagg tgctgggcgc aacgttcagt
    14461 ggatcagacc tctctggcgg cgagttttca tccttcgact ggcgagcagc aaacgttacg
    14521 cactgtgatt tgaccaattc ggaactgggc gatttagata tccgcggggt tgatttgcaa
    14581 ggcgtcaaac tggacagcta ccaggcatcg ttgctcctgg aacgtcttgg tatcgctgtc
    14641 atgggttaat gttcagggag cggtatatgc cgccccctgc ccttatacaa tattgtccgg
    14701 gctggctttc cccctcagac gtttcgactt cgggggcata aacgtcagag ggttccggtc
    14761 cccccgtcag aacaccatac ctgaccggat gtatagctgt tctcttcagc cgctaaagtg
    14821 acatacagcg gagcaatttc taccggttgg ccagggcgac caagcggcgc atttgcacca
    14881 aatttctcaa ttttttcctg tggttggccg ccgcagcact gcaataccgt ccagtacggc
    14941 ccaggcgcga cagcatttac acggatccct ttaggggcca gttgcttcgc cagcgattta
    15001 gggcactgtt gcaaagttag cgatgaggca gccttttgtc t
//
LOCUS       DAZPEU010000651         6146 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.guided.204, whole genome shotgun sequence.
ACCESSION   DAZPEU010000651 DAZPEU010000000
VERSION     DAZPEU010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.guided.204"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            263..922
                     /locus_tag="ACHU2L_004733"
     CDS             263..922
                     /locus_tag="ACHU2L_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR1359214.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACHU2L_004734"
     CDS             complement(1123..1500)
                     /locus_tag="ACHU2L_004734"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1359215.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="ACHU2L_004735"
     CDS             complement(1567..4533)
                     /locus_tag="ACHU2L_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR1359216.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="ACHU2L_004736"
     CDS             complement(4536..5096)
                     /locus_tag="ACHU2L_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1359217.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="ACHU2L_004737"
                     /pseudo
     CDS             complement(5222..5572)
                     /locus_tag="ACHU2L_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(5775..>6146)
                     /gene="intI1"
                     /locus_tag="ACHU2L_004738"
     CDS             complement(5775..>6146)
                     /gene="intI1"
                     /locus_tag="ACHU2L_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1359218.1"
                     /translation="ALERKYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQ
                     RAFKRAVEQAGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVL
                     KVGGAGVRSPLDALPPLTSER"
BASE COUNT         1242 a         1705 c         1847 g         1352 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggc
//
LOCUS       DAZPEU010000652         2973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.guided.309, whole genome shotgun sequence.
ACCESSION   DAZPEU010000652 DAZPEU010000000
VERSION     DAZPEU010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.guided.309"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..640)
                     /gene="intI2"
                     /locus_tag="ACHU2L_004739"
                     /pseudo
     CDS             complement(1..640)
                     /gene="intI2"
                     /locus_tag="ACHU2L_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            972..1445
                     /gene="dfrA1"
                     /locus_tag="ACHU2L_004740"
     CDS             972..1445
                     /gene="dfrA1"
                     /locus_tag="ACHU2L_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1359219.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1540..2064
                     /gene="sat2"
                     /locus_tag="ACHU2L_004741"
     CDS             1540..2064
                     /gene="sat2"
                     /locus_tag="ACHU2L_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1359220.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2122..2960
                     /gene="aadA1"
                     /locus_tag="ACHU2L_004742"
                     /pseudo
     CDS             2122..2960
                     /gene="aadA1"
                     /locus_tag="ACHU2L_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          829 a          658 c          713 g          773 t
ORIGIN      
        1 gtcgataagc agaagggtat ttgtgatcta aagcaaaagg cagcgatggc cctacgcctt
       61 gtaagttgtc gtcttgctga ataagccgcg cttgctcaat gagttatttt attgctggga
      121 ttaggcgcgt gggcagtagg ctgtttctgc ttttcccacc cttaccgtca tgcacagtga
      181 tgcagccatt atcaaaatca aaatctttaa cccgcaaacg caagcattca ttaatgcgca
      241 aacctgcacc atacagcagc gtaaaaataa cttggttgcg agtatccata acctgcaaaa
      301 tgcgttgcac ttcatttgca gagataacag agggtagccg tctaggcttg cttgcaggga
      361 tataatcaat atcgcccaac ggctgttgta aaaacctgtt gtacaaaaaa gctagggcat
      421 ttaaagcgat tttctgcgtg tttatggcta catgtctgct gtttgctaag ctggataaaa
      481 acagcctgac ctcttcactg cccatggtct gaggatgacg ttttttgtga aacagaataa
      541 aacgcttaat ccagtgcagg taagtttttt cagttttcag cgcataacct ttttgtcgca
      601 tatccgtgcg tatagaattt aaaaatggac tgttagacat aaaacgctcc ttgtcttgca
      661 actgtctgcc tatacagcct attctagctg ggatttaaaa aagtgcctgt tttttacgcc
      721 tagagatgct tgtttaccgg tagagtttta atttaatgct aaataaatta aaatgttatg
      781 agttctttgg gtgagataat gtgcatcgtg caagcaggat agacggcatg cacgatttgt
      841 aataacagag tgtcttgtat ttttaaagaa agtctattta atacaagtga ttatattaat
      901 taacggtaag catcagcggg tgacaaaacg agcatgctta ctaataaaat gttaacctct
      961 gaggaagaat tgtgaaacta tcactaatgg tagctatatc gaagaatgga gttatcggga
     1021 atggccctga tattccatgg agtgccaaag gtgaacagct cctgtttaaa gctattacct
     1081 ataaccaatg gctgttggtt ggacgcaaga cttttgaatc aatgggagca ttacccaacc
     1141 gaaagtatgc ggtcgtaaca cgttcaagtt ttacatctga caatgagaac gtattgatct
     1201 ttccatcaat taaagatgct ttaaccaacc taaagaaaat aacggatcat gtcattgttt
     1261 caggtggtgg ggagatatac aaaagcctga tcgatcaagt agatacacta catatatcta
     1321 caatagacat cgagccggaa ggtgatgttt actttcctga aatccccagc aattttaggc
     1381 cagtttttac ccaagacttc gcctctaaca taaattatag ttaccaaatc tggcaaaagg
     1441 gttaacaagt ggcagcaacg gattcgcaaa cctgtcacgc cttttgtacc aaaagccgcg
     1501 ccaggtttgc gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct
     1561 gagcaggtgg cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat
     1621 gtgcacctat ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag
     1681 gattacatct cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac
     1741 caagagcttg tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa
     1801 cacattgttg tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt
     1861 gcgaaaaagt gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac
     1921 aatgtacctg cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg
     1981 ttcacgtata aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc
     2041 tcgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc
     2101 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     2161 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     2221 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     2281 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2341 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2401 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2461 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2521 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2581 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2641 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2701 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2761 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2821 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
     2881 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     2941 cgcttcgcgg cgcggcttaa ctcaagcgtt aga
//
LOCUS       DAZPEU010000653         2970 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.guided.310, whole genome shotgun sequence.
ACCESSION   DAZPEU010000653 DAZPEU010000000
VERSION     DAZPEU010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.guided.310"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(1..640)
                     /gene="intI2"
                     /locus_tag="ACHU2L_004743"
                     /pseudo
     CDS             complement(1..640)
                     /gene="intI2"
                     /locus_tag="ACHU2L_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            972..1445
                     /gene="dfrA1"
                     /locus_tag="ACHU2L_004744"
     CDS             972..1445
                     /gene="dfrA1"
                     /locus_tag="ACHU2L_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1359221.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1540..2064
                     /gene="sat2"
                     /locus_tag="ACHU2L_004745"
     CDS             1540..2064
                     /gene="sat2"
                     /locus_tag="ACHU2L_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1359222.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2122..2957
                     /gene="aadA1"
                     /locus_tag="ACHU2L_004746"
                     /pseudo
     CDS             2122..2957
                     /gene="aadA1"
                     /locus_tag="ACHU2L_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          826 a          658 c          713 g          773 t
ORIGIN      
        1 gtcgataagc agaagggtat ttgtgatcta aagcaaaagg cagcgatggc cctacgcctt
       61 gtaagttgtc gtcttgctga ataagccgcg cttgctcaat gagttatttt attgctggga
      121 ttaggcgcgt gggcagtagg ctgtttctgc ttttcccacc cttaccgtca tgcacagtga
      181 tgcagccatt atcaaaatca aaatctttaa cccgcaaacg caagcattca ttaatgcgca
      241 aacctgcacc atacagcagc gtaaaaataa cttggttgcg agtatccata acctgcaaaa
      301 tgcgttgcac ttcatttgca gagataacag agggtagccg tctaggcttg cttgcaggga
      361 tataatcaat atcgcccaac ggctgttgta aaaacctgtt gtacaaaaaa gctagggcat
      421 ttaaagcgat tttctgcgtg tttatggcta catgtctgct gtttgctaag ctggataaaa
      481 acagcctgac ctcttcactg cccatggtct gaggatgacg ttttttgtga aacagaataa
      541 aacgcttaat ccagtgcagg taagtttttt cagttttcag cgcataacct ttttgtcgca
      601 tatccgtgcg tatagaattt aaaaatggac tgttagacat aaaacgctcc ttgtcttgca
      661 actgtctgcc tatacagcct attctagctg ggatttaaaa aagtgcctgt tttttacgcc
      721 tagagatgct tgtttaccgg tagagtttta atttaatgct aaataaatta aaatgttatg
      781 agttctttgg gtgagataat gtgcatcgtg caagcaggat agacggcatg cacgatttgt
      841 aataacagag tgtcttgtat ttttaaagaa agtctattta atacaagtga ttatattaat
      901 taacggtaag catcagcggg tgacaaaacg agcatgctta ctaataaaat gttaacctct
      961 gaggaagaat tgtgaaacta tcactaatgg tagctatatc gaagaatgga gttatcggga
     1021 atggccctga tattccatgg agtgccaaag gtgaacagct cctgtttaaa gctattacct
     1081 ataaccaatg gctgttggtt ggacgcaaga cttttgaatc aatgggagca ttacccaacc
     1141 gaaagtatgc ggtcgtaaca cgttcaagtt ttacatctga caatgagaac gtattgatct
     1201 ttccatcaat taaagatgct ttaaccaacc taaagaaaat aacggatcat gtcattgttt
     1261 caggtggtgg ggagatatac aaaagcctga tcgatcaagt agatacacta catatatcta
     1321 caatagacat cgagccggaa ggtgatgttt actttcctga aatccccagc aattttaggc
     1381 cagtttttac ccaagacttc gcctctaaca taaattatag ttaccaaatc tggcaaaagg
     1441 gttaacaagt ggcagcaacg gattcgcaaa cctgtcacgc cttttgtacc aaaagccgcg
     1501 ccaggtttgc gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct
     1561 gagcaggtgg cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat
     1621 gtgcacctat ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag
     1681 gattacatct cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac
     1741 caagagcttg tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa
     1801 cacattgttg tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt
     1861 gcgaaaaagt gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac
     1921 aatgtacctg cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg
     1981 ttcacgtata aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc
     2041 tcgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc
     2101 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     2161 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     2221 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     2281 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2341 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2401 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2461 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2521 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2581 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2641 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2701 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2761 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga
     2821 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
     2881 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
     2941 ttcgcggcgc ggcttaactc aagcgttaga
//
LOCUS       DAZPEU010000654         2711 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.guided.332, whole genome shotgun sequence.
ACCESSION   DAZPEU010000654 DAZPEU010000000
VERSION     DAZPEU010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.guided.332"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            <1..231
                     /locus_tag="ACHU2L_004747"
     CDS             <1..231
                     /locus_tag="ACHU2L_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HFR1359223.1"
                     /translation="AWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIV
                     KDEKMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            242..601
                     /gene="frdD"
                     /locus_tag="ACHU2L_004748"
     CDS             242..601
                     /gene="frdD"
                     /locus_tag="ACHU2L_004748"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1359224.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            664..1796
                     /locus_tag="ACHU2L_004749"
                     /pseudo
     CDS             664..1796
                     /locus_tag="ACHU2L_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            1885..2418
                     /gene="blc"
                     /locus_tag="ACHU2L_004750"
     CDS             1885..2418
                     /gene="blc"
                     /locus_tag="ACHU2L_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1359225.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2415..>2711)
                     /gene="sugE"
                     /locus_tag="ACHU2L_004751"
     CDS             complement(2415..>2711)
                     /gene="sugE"
                     /locus_tag="ACHU2L_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1359226.1"
                     /translation="IAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMALLAWAMK
                     SLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKLSTH"
BASE COUNT          657 a          711 c          694 g          649 t
ORIGIN      
        1 gcctgggcgg gattcgtcga ctttttacaa aacccggtta tcgtgatcat taacctgatc
       61 actctgacgg cagctctgct gcacaccaaa acctggtttg aactggcacc gaaagcggcc
      121 aatatcattg taaaagacga aaaaatggga ccagagccaa ttatcaaaag tctctgggcg
      181 gtaactgtgg ttgccaccat cgtaatcctg tttgttgccc tgtactggta aggagcctga
      241 gatgattaat ccaaatccaa agcgttctga cgaaccggta ttctggggcc tcttcggggc
      301 cggtggtatg tggagcgcaa ttattgcgcc ggtgatgatc ctgctggtgg gtattctgct
      361 gccactgggg ctgtttccgg gtgatgcgct gagctacgag cgcgttctgg cgttcgcgca
      421 gagcttcatt ggtcgcgtat tcctgttcct gatgatcgtt ctgccgctgt ggtgtggttt
      481 acaccgtatg caccacgcga tgcacgatct gaaaatccac gtgcctgcgg gcaaatgggt
      541 tttctacggt ctggctgcta tcctgacagt tgtcacgctg attggtgtcg ttacaatcta
      601 acgcatcgcc aatgtaaatc cggcccgcct atggcgggcc gttttgtatg gaaaccagac
      661 cttatgttca aaacgacgct ctgcgcctta ttaattaccg cctcttactc cacatttgct
      721 gcccctcaac aaatcaacga tattgtgcat cgcacaatta ccccgcttat agagcaacaa
      781 aagatccccg gtatggcggt ggcggtaatt tatcagggta aaccttatta ctttacctgg
      841 ggctatgcgg acatcgccaa aaagcagccc gtcacacagc aaacgttgtt tgagttaggt
      901 tcggtcagca aaacatttac gggcgtgctt ggtggcgacg ctattgctcg aggggaaatc
      961 aagttaagcg atcccgcaac aaaatactgg cctgaactta ccgctaaaca gtggaatggg
     1021 atcacactat tacatcttgc gacctacacc gctggcggcc tgccattgca ggtgccggat
     1081 gaagtgaaat cctcaagcga cttgctgcgc ttctatcaaa actggcagcc tgcatgggct
     1141 ccaggaacac aacgtctgta tgccaactcc agtatcggtt tgttcggcgc actggctgtg
     1201 aagccgtctg gtttgagttt tgagcaggcg atgcaaactc gtgtcttcca gccactcaaa
     1261 ctcaaccata cgtggattaa tgtacctccc ccagaagaaa agaattacgc ctggggatat
     1321 cgcgaaggta aggcagtgca tgtttcgcca ggggcgttag atgctgaagc ttatggtgtg
     1381 aagtcgacca ttgaagatat ggcccgctgg gtgcaaagca atttaccccc cttgatatca
     1441 atgagaaaac gcttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg
     1501 atatgtatca gggcctgggc tgggaaatgc tggactggcc ggtcaatcct gacagcatca
     1561 ttaacggcag tgacaataaa attgcactgg cagcacgccc ggtaaaagcg attaagcccc
     1621 caactcctgc agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta
     1681 gctatgtcgc gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact
     1741 atcccaatcc agcgagagtc gccgccgcct gtcagattct caacgctcta cagtaaaatt
     1801 tcatcgggtc cgaattttcg gaccttttct gcgcttttcc ttgctgtcat ctacacttag
     1861 aaaaaaacca gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct
     1921 gcatttctgg tcgttgcctg tagttttcct acgccgccgc gtggcgtgac cgtagtgaat
     1981 aatttcgacg ccaaacgtta tctcggtacc tggtatgaga ttgcccgttt tgatcaccgt
     2041 tttgaacgtg gactggaaaa agtcaccgca acatacagcc tgcgtgatga cggcggcctg
     2101 aatgtcatta ataaaggcta taaccctgac agaggaatgt ggcagcagag tgaagggaaa
     2161 gcgtacttta ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt tggtcctttc
     2221 tatggcggtt ataacgttat tgcactcgat cgggaatacc gccatgcgct ggtttgcggc
     2281 ccggaccgcg actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa
     2341 caggagatgc tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta
     2401 cagcagcccg gtagttagtg agtgctgagt ttcagaccaa taatccccaa tacgattaac
     2461 gccagactcg ccagacgcat cgggttagcg gactcaccga gcagcacaat gccggtgatg
     2521 gccgcgccga ctgcgccaat acccgtccac acggcataag ccgtccctac cggtaacgat
     2581 ttcatcgccc aggcaagtag cgccatactg actatcatcg ccgtcacggt aataacactc
     2641 ggcgtcaaac ggctaaagcc gtgggtatat ttcaggccaa cggcccatac cacttccagc
     2701 agaccagcaa t
//
LOCUS       DAZPEU010000655         2498 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.guided.340, whole genome shotgun sequence.
ACCESSION   DAZPEU010000655 DAZPEU010000000
VERSION     DAZPEU010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.guided.340"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..858)
                     /gene="pepD"
                     /locus_tag="ACHU2L_004752"
     CDS             complement(<1..858)
                     /gene="pepD"
                     /locus_tag="ACHU2L_004752"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HFR1359227.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEI"
     gene            1120..1578
                     /gene="gpt"
                     /locus_tag="ACHU2L_004753"
     CDS             1120..1578
                     /gene="gpt"
                     /locus_tag="ACHU2L_004753"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1359228.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1670..>2498
                     /gene="frsA"
                     /locus_tag="ACHU2L_004754"
     CDS             1670..>2498
                     /gene="frsA"
                     /locus_tag="ACHU2L_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1359229.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     "
BASE COUNT          587 a          650 c          648 g          613 t
ORIGIN      
        1 gatctcctga taggtattca ccagagattt cagtgcgtcg actttatcag ctgcgacagc
       61 aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt tgaaatcgat
      121 aaggcgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccagca gtttgttggc
      181 attacccagg ccaacgtgga tttcaccgcc ggagtgaccg cctttcagac cttttaaggt
      241 taacttgaag gtttcaaaac cagctggaac cgcttcacga tctaaatgca ggttggaggt
      301 gaagtcgata ccccccgcac aacccatgta gatttcacct tcttcttcgg agtcggtgtt
      361 aatcagaata tcagcctgca accagttgct ctgtaagccg aacgcaccgt ccataccggc
      421 ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag
      481 aaccgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg tgccgcgcgc
      541 tttaacccat tcgccatcaa tataaggctg gataggatct ttcgtgaagt catgcacggt
      601 gtcgttattt ttctgcggca ccatatcgag gtgggcctgt aagacgaccg gtttacgatt
      661 ttccatacct gcggtagcag gttgacgaat caggatatta cctacctgat cgcgttcgac
      721 atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata
      781 ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa
      841 ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg
      901 tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtattgaga
      961 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
     1021 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
     1081 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
     1141 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
     1201 caatggaaag gcattattgc cgtaagccgt ggcggtctgg taccgggggc gttactggcg
     1261 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     1321 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     1381 gatgacctgg tggataccgg tggtactgct gttgcgattc gtgaaatgta tccaaaagcg
     1441 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     1501 gatatcccgc aagatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg
     1561 ccaatctccg gtcgctaatc ttttcaacgc ctggcattgc cgggcgttgt tctttttaac
     1621 ttcaggcagg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     1681 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     1741 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gctcttgatg gtaaaaccat
     1801 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggta ttgacccacg
     1861 tgaaatcctc gacgtccagg cacgtattgt gatgagcgat gctgaacgta ccgacgatga
     1921 tttatacgat accgtgattg gctaccgtgg tggcaactgg atttatgagt gggccaccca
     1981 ggcgatggtg tggcaacaaa aagcctgtac ggaagaagat ccgcaactca gtggtcgaca
     2041 ctggctgcat gcggctacgt tgtacaacat tgccgcctat cctcatctga aaggagatga
     2101 cctggccgag caagcgcagg ctttatcaaa ccgcgcctat gaagaggccg cccagcgtct
     2161 gccgggcacg atgcggcaga tggagtttac cgtacccggc ggtgcgccca tcaccggctt
     2221 tttgcatatg ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg gtggtctgga
     2281 tgcgatgcag acggactatt acagcctgta tgaacgttat tttgcgccgc gcggcattgc
     2341 gatgctgact attgatatgc cgtcggtggg cttttcttca aaatggaagc tcacccagga
     2401 ctccagcctg ttgcatcagc acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca
     2461 cactcgcgtc gcggcctttg gtttccgttt cggcgcta
//
LOCUS       DAZPEU010000656         2316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.guided.350, whole genome shotgun sequence.
ACCESSION   DAZPEU010000656 DAZPEU010000000
VERSION     DAZPEU010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.guided.350"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            complement(<1..352)
                     /gene="tetR(B)"
                     /locus_tag="ACHU2L_004755"
     CDS             complement(<1..352)
                     /gene="tetR(B)"
                     /locus_tag="ACHU2L_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR1359230.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQL"
     gene            434..1639
                     /gene="tet(B)"
                     /locus_tag="ACHU2L_004756"
     CDS             434..1639
                     /gene="tet(B)"
                     /locus_tag="ACHU2L_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR1359231.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1752..>2316)
                     /gene="tetC"
                     /locus_tag="ACHU2L_004757"
     CDS             complement(1752..>2316)
                     /gene="tetC"
                     /locus_tag="ACHU2L_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR1359232.1"
                     /translation="FKSTYQSLVNSARILFVEKGYQAVSIDEISGKALVTKGAFYHHF
                     KNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNKNLIPIQE
                     VMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAIHA
                     KNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          602 a          435 c          479 g          800 t
ORIGIN      
        1 ctaattgatt ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct aaatgtactt
       61 ttgctccatc gcgatgactt agtaaagcac atctaaaact tttagcgtta ttacgtaaaa
      121 aatcttgcca gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct
      181 caatggctaa ggcgtcgagc aaagcccgct tattttttac atgccaatac aatgtaggct
      241 gctctacacc tagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat
      301 taagcagctc taatgcgctg ttaatcactt tacttttatc taatctagac atcattaatt
      361 cctaattttt gttgacactc tatcattgat agagttattt taccactccc tatcagtgat
      421 agagaaaagt gaaatgaata gttcgacaaa gatcgcattg gtaattacgt tactcgatgc
      481 catggggatt ggccttatca tgccagtctt gccaacgtta ttacgtgaat ttattgcttc
      541 ggaagatatc gctaaccact ttggcgtatt gcttgcactt tatgcgttaa tgcaggttat
      601 ctttgctcct tggcttggaa aaatgtctga ccgatttggt cggcgcccag tgctgttgtt
      661 gtcattaata ggcgcatcgc tggattactt attgctggct ttttcaagtg cgctttggat
      721 gctgtattta ggccgtttgc tttcagggat cacaggagct actggggctg tcgcggcatc
      781 ggtcattgcc gataccacct cagcttctca acgcgtgaag tggttcggtt ggttaggggc
      841 aagttttggg cttggtttaa tagcggggcc tattattggt ggttttgcag gagagatttc
      901 accgcatagt ccctttttta tcgctgcgtt gctaaatatt gtcgctttcc ttgtggttat
      961 gttttggttc cgtgaaacca aaaatacacg tgataataca gataccgaag taggggttga
     1021 gacgcaatcg aattcggtat acatcacttt atttaaaacg atgcccattt tgttgattat
     1081 ttatttttca gcgcaattga taggccaaat tcccgcaacg gtgtgggtgc tatttaccga
     1141 aaatcgtttt ggatggaata gcatgatggt tggcttttca ttagcgggtc ttggtctttt
     1201 acactcagta ttccaagcct ttgtggcagg aagaatagcc actaaatggg gcgaaaaaac
     1261 ggcagtactg ctcggattta ttgcagatag tagtgcattt gcctttttag cgtttatatc
     1321 tgaaggttgg ttagttttcc ctgttttaat tttattggct ggtggtggga tcgctttacc
     1381 tgcattacag ggagtgatgt ctatccaaac aaagagtcat cagcaaggtg ctttacaggg
     1441 attattggtg agccttacca atgcaaccgg tgttattggc ccattactgt ttgctgttat
     1501 ttataatcat tcactaccaa tttgggatgg ctggatttgg attattggtt tagcgtttta
     1561 ctgtattatt atcctgctat cgatgacctt catgttaacc cctcaagctc aggggagtaa
     1621 acaggagaca agtgcttagt tatttcgtca ccaaatgatg ttattccgcg aaatataatg
     1681 accctcttga taacccaaga gggcattttt tacgataaag aagatttagc ttcaaataaa
     1741 acctatctat tttatttatc tttcaagctc aataaaaagc cgcggtaaat agcaataaat
     1801 tggccttttt tatcggcaag ctcttttagg tttttcgcat gtattgcgat atgcataaac
     1861 cagccattga gtaagttttt aagcacatca tcatcataag ctttaagttg gttctcttgg
     1921 atcaatttgc tgacaatggc gtttacctta ccagtaatgt attcaaggct aattttttca
     1981 agttcattcc aaccaatgat aggcatcact tcttggatag ggataaggtt tttattatta
     2041 tcaataatat aatcaagata atgttcaaat atactttcta aggcagacca accatttgtt
     2101 aaatcagttt ttgttgtgat gtaggcatca atcataatta attgctgctt ataacaggca
     2161 ctgagtaatt gttttttatt tttaaagtga tgataaaagg cacctttggt caccaacgct
     2221 tttcccgaga tctcatctat tgaaacagct tgatagcctt tttcaacaaa caatattcgt
     2281 gctgagttaa ccagtgattg ataggtactc ttaaaa
//
LOCUS       DAZPEU010000657         1109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.guided.455, whole genome shotgun sequence.
ACCESSION   DAZPEU010000657 DAZPEU010000000
VERSION     DAZPEU010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.guided.455"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            136..609
                     /gene="dfrA17"
                     /locus_tag="ACHU2L_004758"
     CDS             136..609
                     /gene="dfrA17"
                     /locus_tag="ACHU2L_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR1359233.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            740..1066
                     /locus_tag="ACHU2L_004759"
                     /pseudo
     CDS             740..1066
                     /locus_tag="ACHU2L_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
BASE COUNT          299 a          244 c          268 g          298 t
ORIGIN      
        1 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
       61 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttagcc
      121 attaagggag ttaaattgaa aatatcattg atttctgcag tgtcagaaaa tggcgtaatc
      181 ggtagtggtc ctgatatccc gtggtcagta aaaggtgagc aactactctt taaagcgctc
      241 acatataatc aatggctcct tgtcggaaga aaaacatttg actctatggg tgttcttcca
      301 aatcgcaaat atgcagtagt gtcaaagaac ggaatttcaa gctcaaatga aaacgtccta
      361 gtttttcctt caatagaaaa tgctttgaaa gagctatcaa aagttacaga tcatgtatat
      421 gtctctggcg ggggtcaaat ctataatagc cttattgaaa aagcagatat aattcatttg
      481 tctactgttc acgttgaagt cgaaggtgat atcaaattcc ctataatgcc tgagaatttc
      541 aatttggttt ttgaacagtt ttttatgtct aatataaatt atacatacca gatttggaaa
      601 aaaggctaac aatgcgttgc agcaccagtc gcttcgctcc ttggacagct tttaagtcgc
      661 gtctttgtgg ttttgctgcg caaaagtatt ccacaaagcc gcaacttaaa agctgccgct
      721 gaacttaacg ttaggcatca tgggtgaatt tttccctgca caagttttca agcagctgtc
      781 ccacgctcgc gcggtgatcg agcgccatct ggctgcgaca ctggacacaa tccacctgtt
      841 cggatctgcg atcgatggag ggctgaagcc ggacagcgac atagacttgc tcgtgaccgt
      901 cagcgccgca cctaacgatt cgctccggca ggcgctaatg ctcgatttgc tgaaagtctc
      961 atcaccgcca ggcgatggcg gaacatggcg accgctggag ctaactgttg tcgctcgaag
     1021 cgaagtagtg ccttggcgct atccggcgcg gcgtgagctt cagttcggtg ggcactgttg
     1081 caaatagtcg gtggtgataa acttatcat
//
LOCUS       DAZPEU010000658          990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.guided.468, whole genome shotgun sequence.
ACCESSION   DAZPEU010000658 DAZPEU010000000
VERSION     DAZPEU010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.guided.468"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            139..977
                     /gene="aadA1"
                     /locus_tag="ACHU2L_004760"
                     /pseudo
     CDS             139..977
                     /gene="aadA1"
                     /locus_tag="ACHU2L_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          251 a          253 c          261 g          225 t
ORIGIN      
        1 ccattctaaa cacactaaat ttataaaaaa tctaatggca aaatcgccca acccttcaat
       61 caagtcggga cggccaaaag caagcttttg gctcccctcg ctggcgctcg gcgcccctta
      121 tttcaaacgt taaacatcat gagggaagtg gtgatcgccg aagtatcgac tcaactatca
      181 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
      241 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
      301 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
      361 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
      421 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
      481 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
      541 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
      601 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
      661 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
      721 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
      781 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
      841 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
      901 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
      961 ttcgcggcgc ggcttaactc aagcgttaga
//
LOCUS       DAZPEU010000659          987 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M1906223
            SAMN43277371-rid23489813.guided.470, whole genome shotgun sequence.
ACCESSION   DAZPEU010000659 DAZPEU010000000
VERSION     DAZPEU010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277371
            Sequence Read Archive: SRR30464587
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396938.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396938.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:52:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,189
            CDSs (with protein)               :: 4,189
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 490
            CDSs (without protein)            :: 490
            Pseudo Genes (ambiguous residues) :: 0 of 490
            Pseudo Genes (frameshifted)       :: 232 of 490
            Pseudo Genes (incomplete)         :: 161 of 490
            Pseudo Genes (internal stop)      :: 168 of 490
            Pseudo Genes (multiple problems)  :: 66 of 490
            Pseudo Genes (short protein)      :: 1 of 490
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277371-rid23489813.guided.470"
                     /strain="M1906223"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-06-01"
                     /collected_by="FSS"
     gene            139..974
                     /gene="aadA1"
                     /locus_tag="ACHU2L_004761"
                     /pseudo
     CDS             139..974
                     /gene="aadA1"
                     /locus_tag="ACHU2L_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          248 a          253 c          261 g          225 t
ORIGIN      
        1 ccattctaaa cacactaaat ttataaaaaa tctaatggca aaatcgccca acccttcaat
       61 caagtcggga cggccaaaag caagcttttg gctcccctcg ctggcgctcg gcgcccctta
      121 tttcaaacgt taaacatcat gagggaagtg gtgatcgccg aagtatcgac tcaactatca
      181 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
      241 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
      301 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
      361 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
      421 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
      481 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
      541 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
      601 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
      661 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
      721 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
      781 cgcctgccgg cccagtatca gcccgtcata cttgaagcta ggcaggctta tcttggacaa
      841 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc
      901 gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc
      961 gcggcgcggc ttaactcaag cgttaga
//
